LOCUS       DACDCJ010000001        99945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDCJ010000001 DACDCJ010000000
VERSION     DACDCJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 99945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 99945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.001"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(554..2167)
                     /locus_tag="IV038_000001"
     CDS             complement(554..2167)
                     /locus_tag="IV038_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP8340259.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="IV038_000002"
     CDS             complement(2308..3216)
                     /locus_tag="IV038_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8340260.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3399..4523
                     /locus_tag="IV038_000003"
     CDS             3399..4523
                     /locus_tag="IV038_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8340261.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4750..5667
                     /locus_tag="IV038_000004"
     CDS             4750..5667
                     /locus_tag="IV038_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8340262.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5660..6493
                     /locus_tag="IV038_000005"
     CDS             5660..6493
                     /locus_tag="IV038_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8340263.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6576..7694
                     /locus_tag="IV038_000006"
     CDS             6576..7694
                     /locus_tag="IV038_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8340264.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7688..9586
                     /locus_tag="IV038_000007"
     CDS             7688..9586
                     /locus_tag="IV038_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP8340265.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIEKCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9580..10803
                     /locus_tag="IV038_000008"
     CDS             9580..10803
                     /locus_tag="IV038_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP8340266.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWVRGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10889..12823)
                     /locus_tag="IV038_000009"
     CDS             complement(10889..12823)
                     /locus_tag="IV038_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8340267.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13049..13705
                     /locus_tag="IV038_000010"
     CDS             13049..13705
                     /locus_tag="IV038_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8340268.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13779..14132)
                     /locus_tag="IV038_000011"
     CDS             complement(13779..14132)
                     /locus_tag="IV038_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8340269.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14324..15253
                     /locus_tag="IV038_000012"
     CDS             14324..15253
                     /locus_tag="IV038_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP8340270.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15264..16100
                     /locus_tag="IV038_000013"
     CDS             15264..16100
                     /locus_tag="IV038_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP8340271.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16348..17124
                     /locus_tag="IV038_000014"
     CDS             16348..17124
                     /locus_tag="IV038_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8340272.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17300..18043
                     /locus_tag="IV038_000015"
     CDS             17300..18043
                     /locus_tag="IV038_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340273.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18036..19616
                     /locus_tag="IV038_000016"
     CDS             18036..19616
                     /locus_tag="IV038_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP8340274.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19867..20346)
                     /locus_tag="IV038_000017"
     CDS             complement(19867..20346)
                     /locus_tag="IV038_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP8340275.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20362..21114)
                     /locus_tag="IV038_000018"
     CDS             complement(20362..21114)
                     /locus_tag="IV038_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP8340276.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21231..21301
                     /locus_tag="IV038_000019"
     tRNA            21231..21301
                     /locus_tag="IV038_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21263..21265,aa:Gly,seq:ccc)
     gene            21662..22387
                     /locus_tag="IV038_000020"
     CDS             21662..22387
                     /locus_tag="IV038_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP8340277.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22642..22908
                     /locus_tag="IV038_000021"
     CDS             22642..22908
                     /locus_tag="IV038_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP8340278.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22905..23336
                     /gene="ruvX"
                     /locus_tag="IV038_000022"
     CDS             22905..23336
                     /gene="ruvX"
                     /locus_tag="IV038_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP8340279.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23361..23666
                     /locus_tag="IV038_000023"
     CDS             23361..23666
                     /locus_tag="IV038_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP8340280.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23747..24892)
                     /locus_tag="IV038_000024"
     CDS             complement(23747..24892)
                     /locus_tag="IV038_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8340281.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24953..25600)
                     /locus_tag="IV038_000025"
     CDS             complement(24953..25600)
                     /locus_tag="IV038_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8340282.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25794..26087
                     /locus_tag="IV038_000026"
     CDS             25794..26087
                     /locus_tag="IV038_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340283.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26131..26442
                     /locus_tag="IV038_000027"
     CDS             26131..26442
                     /locus_tag="IV038_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340284.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26439..27063
                     /locus_tag="IV038_000028"
                     /pseudo
     CDS             26439..27063
                     /locus_tag="IV038_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27363..27725
                     /locus_tag="IV038_000029"
     CDS             27363..27725
                     /locus_tag="IV038_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340285.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27762..28610
                     /locus_tag="IV038_000030"
     CDS             27762..28610
                     /locus_tag="IV038_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP8340286.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28600..30075
                     /locus_tag="IV038_000031"
     CDS             28600..30075
                     /locus_tag="IV038_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP8340287.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30341..30748
                     /locus_tag="IV038_000032"
     CDS             30341..30748
                     /locus_tag="IV038_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP8340288.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30751..30966
                     /locus_tag="IV038_000033"
     CDS             30751..30966
                     /locus_tag="IV038_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340289.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30970..31350
                     /locus_tag="IV038_000034"
     CDS             30970..31350
                     /locus_tag="IV038_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8340290.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31484..31639
                     /locus_tag="IV038_000035"
     CDS             31484..31639
                     /locus_tag="IV038_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP8340291.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31708..32181
                     /locus_tag="IV038_000036"
     CDS             31708..32181
                     /locus_tag="IV038_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP8340292.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32178..33872
                     /locus_tag="IV038_000037"
     CDS             32178..33872
                     /locus_tag="IV038_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8340293.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33838..34023
                     /locus_tag="IV038_000038"
     CDS             33838..34023
                     /locus_tag="IV038_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340294.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34027..35202
                     /locus_tag="IV038_000039"
     CDS             34027..35202
                     /locus_tag="IV038_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8340295.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35195..36718
                     /locus_tag="IV038_000040"
     CDS             35195..36718
                     /locus_tag="IV038_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8340296.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36774..37058
                     /locus_tag="IV038_000041"
     CDS             36774..37058
                     /locus_tag="IV038_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP8340297.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37045..37380
                     /locus_tag="IV038_000042"
     CDS             37045..37380
                     /locus_tag="IV038_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8340298.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37675..38301
                     /locus_tag="IV038_000043"
     CDS             37675..38301
                     /locus_tag="IV038_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340299.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39043..39879
                     /locus_tag="IV038_000044"
     CDS             39043..39879
                     /locus_tag="IV038_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8340300.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39944..40378
                     /locus_tag="IV038_000045"
     CDS             39944..40378
                     /locus_tag="IV038_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340301.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40446..41768)
                     /locus_tag="IV038_000046"
     CDS             complement(40446..41768)
                     /locus_tag="IV038_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP8340302.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41922..43562)
                     /locus_tag="IV038_000047"
     CDS             complement(41922..43562)
                     /locus_tag="IV038_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP8340303.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43892..44362
                     /locus_tag="IV038_000048"
     CDS             43892..44362
                     /locus_tag="IV038_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP8340304.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44991..46094
                     /locus_tag="IV038_000049"
     CDS             44991..46094
                     /locus_tag="IV038_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8340305.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46378..47058
                     /locus_tag="IV038_000050"
     CDS             46378..47058
                     /locus_tag="IV038_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8340306.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47148..48182
                     /locus_tag="IV038_000051"
     CDS             47148..48182
                     /locus_tag="IV038_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP8340307.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48196..48531
                     /locus_tag="IV038_000052"
     CDS             48196..48531
                     /locus_tag="IV038_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340308.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48515..50995
                     /locus_tag="IV038_000053"
     CDS             48515..50995
                     /locus_tag="IV038_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340309.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51051..52610
                     /locus_tag="IV038_000054"
     CDS             51051..52610
                     /locus_tag="IV038_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8340310.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52607..53971
                     /locus_tag="IV038_000055"
     CDS             52607..53971
                     /locus_tag="IV038_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8340311.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54142..55212
                     /locus_tag="IV038_000056"
     CDS             54142..55212
                     /locus_tag="IV038_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340312.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55227..55895
                     /locus_tag="IV038_000057"
     CDS             55227..55895
                     /locus_tag="IV038_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340313.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55915..56493
                     /locus_tag="IV038_000058"
     CDS             55915..56493
                     /locus_tag="IV038_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8340314.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56896..57807
                     /locus_tag="IV038_000059"
     CDS             56896..57807
                     /locus_tag="IV038_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8340315.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58063..58980)
                     /locus_tag="IV038_000060"
     CDS             complement(58063..58980)
                     /locus_tag="IV038_000060"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP8340316.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59182..59619
                     /gene="zapA"
                     /locus_tag="IV038_000061"
     CDS             59182..59619
                     /gene="zapA"
                     /locus_tag="IV038_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP8340317.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59677..60225
                     /locus_tag="IV038_000062"
     CDS             59677..60225
                     /locus_tag="IV038_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP8340318.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            60340..62700
                     /locus_tag="IV038_000063"
     CDS             60340..62700
                     /locus_tag="IV038_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP8340319.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62787..63101
                     /gene="trxA"
                     /locus_tag="IV038_000064"
     CDS             62787..63101
                     /gene="trxA"
                     /locus_tag="IV038_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP8340320.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63283..65061
                     /gene="uvrC"
                     /locus_tag="IV038_000065"
     CDS             63283..65061
                     /gene="uvrC"
                     /locus_tag="IV038_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP8340321.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65206..66105
                     /locus_tag="IV038_000066"
     CDS             65206..66105
                     /locus_tag="IV038_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340322.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66098..67360
                     /locus_tag="IV038_000067"
     CDS             66098..67360
                     /locus_tag="IV038_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340323.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67523..69421
                     /locus_tag="IV038_000068"
     CDS             67523..69421
                     /locus_tag="IV038_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP8340324.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69720..70439)
                     /locus_tag="IV038_000069"
     CDS             complement(69720..70439)
                     /locus_tag="IV038_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP8340325.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70776..71108
                     /locus_tag="IV038_000070"
     CDS             70776..71108
                     /locus_tag="IV038_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340326.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71095..73098
                     /locus_tag="IV038_000071"
     CDS             71095..73098
                     /locus_tag="IV038_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP8340327.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73117..73587
                     /locus_tag="IV038_000072"
     CDS             73117..73587
                     /locus_tag="IV038_000072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP8340328.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73608..74192
                     /locus_tag="IV038_000073"
     CDS             73608..74192
                     /locus_tag="IV038_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP8340329.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74207..75193
                     /locus_tag="IV038_000074"
     CDS             74207..75193
                     /locus_tag="IV038_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP8340330.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75183..75494
                     /locus_tag="IV038_000075"
     CDS             75183..75494
                     /locus_tag="IV038_000075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP8340331.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75516..77297
                     /locus_tag="IV038_000076"
     CDS             75516..77297
                     /locus_tag="IV038_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP8340332.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77290..78666
                     /locus_tag="IV038_000077"
     CDS             77290..78666
                     /locus_tag="IV038_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP8340333.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGQPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78673..79308
                     /locus_tag="IV038_000078"
     CDS             78673..79308
                     /locus_tag="IV038_000078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP8340334.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79338..80693
                     /locus_tag="IV038_000079"
     CDS             79338..80693
                     /locus_tag="IV038_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP8340335.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80885..82279)
                     /locus_tag="IV038_000080"
     CDS             complement(80885..82279)
                     /locus_tag="IV038_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8340336.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82371..83342
                     /locus_tag="IV038_000081"
     CDS             82371..83342
                     /locus_tag="IV038_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8340337.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83339..84121
                     /locus_tag="IV038_000082"
     CDS             83339..84121
                     /locus_tag="IV038_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP8340338.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84290..84661)
                     /locus_tag="IV038_000083"
     CDS             complement(84290..84661)
                     /locus_tag="IV038_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8340339.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84795..85238
                     /locus_tag="IV038_000084"
     CDS             84795..85238
                     /locus_tag="IV038_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8340340.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85281..86090)
                     /gene="yidA"
                     /locus_tag="IV038_000085"
     CDS             complement(85281..86090)
                     /gene="yidA"
                     /locus_tag="IV038_000085"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP8340341.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86109..87476)
                     /locus_tag="IV038_000086"
     CDS             complement(86109..87476)
                     /locus_tag="IV038_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8340342.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87460..88290)
                     /locus_tag="IV038_000087"
     CDS             complement(87460..88290)
                     /locus_tag="IV038_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP8340343.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88453..88890
                     /locus_tag="IV038_000088"
     CDS             88453..88890
                     /locus_tag="IV038_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP8340344.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88966..89586
                     /locus_tag="IV038_000089"
     CDS             88966..89586
                     /locus_tag="IV038_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP8340345.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89775..90281
                     /locus_tag="IV038_000090"
     CDS             89775..90281
                     /locus_tag="IV038_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP8340346.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            90298..91197
                     /locus_tag="IV038_000091"
     CDS             90298..91197
                     /locus_tag="IV038_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP8340347.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            91284..92168
                     /locus_tag="IV038_000092"
     CDS             91284..92168
                     /locus_tag="IV038_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8340348.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(92360..92431)
                     /locus_tag="IV038_000093"
     tRNA            complement(92360..92431)
                     /locus_tag="IV038_000093"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92396..92398),aa:Arg,seq:ccg)
     gene            92491..93825
                     /gene="rpoN"
                     /locus_tag="IV038_000094"
     CDS             92491..93825
                     /gene="rpoN"
                     /locus_tag="IV038_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP8340349.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            94028..95065
                     /locus_tag="IV038_000095"
     CDS             94028..95065
                     /locus_tag="IV038_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP8340350.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            95106..96107
                     /gene="gap"
                     /locus_tag="IV038_000096"
     CDS             95106..96107
                     /gene="gap"
                     /locus_tag="IV038_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8340351.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            96217..97407
                     /locus_tag="IV038_000097"
     CDS             96217..97407
                     /locus_tag="IV038_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP8340352.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            97511..98266
                     /locus_tag="IV038_000098"
     CDS             97511..98266
                     /locus_tag="IV038_000098"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP8340353.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            98382..99680
                     /gene="eno"
                     /locus_tag="IV038_000099"
     CDS             98382..99680
                     /gene="eno"
                     /locus_tag="IV038_000099"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP8340354.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        33163 a        17067 c        20602 g        29113 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     3421 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     3481 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     3541 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     3601 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     3661 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     3721 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     3781 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     3841 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     3901 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     3961 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     4021 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     4081 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     4141 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     4201 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     4261 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     4321 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcca
     4381 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     4441 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     4501 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     4561 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     4621 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     4681 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     4741 gacttaacga tgatgcaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     4801 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     4861 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     4921 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     4981 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     5041 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     5101 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     5161 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     5221 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     5281 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     5341 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     5401 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     5461 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     5521 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     5581 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     5641 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     5701 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     5761 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     5821 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     5881 gattgttatt gctgtaatca gtacagtttt agctacagcc agttctacaa tggcgggtta
     5941 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     6001 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     6061 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     6121 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     6181 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     6241 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     6301 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     6361 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     6421 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     6481 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     6541 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     6601 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     6661 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     6721 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     6781 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     6841 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     6901 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     6961 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     7021 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     7081 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     7141 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     7201 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     7261 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     7321 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     7381 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     7441 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     7501 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     7561 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     7621 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     7681 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     7741 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     7801 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     7861 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     7921 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     7981 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     8041 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     8101 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     8161 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     8221 atcagcactg aaagaagcat ttgataatat agaaaaatgt tatcttcaaa atggctggta
     8281 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     8341 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     8401 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     8461 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     8521 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     8581 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     8641 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     8701 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     8761 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     8821 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     8881 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     8941 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     9001 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     9061 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     9121 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     9181 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     9241 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     9301 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     9361 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     9421 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     9481 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     9541 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     9601 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     9661 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     9721 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     9781 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     9841 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     9901 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     9961 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
    10021 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
    10081 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
    10141 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
    10201 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
    10261 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
    10321 caaggttata gtgataattc ctgttgggtt agaggccaag catgggggat ttacggtttt
    10381 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
    10441 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
    10501 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
    10561 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
    10621 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
    10681 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
    10741 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
    10801 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
    10861 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
    10921 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
    10981 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
    11041 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
    11101 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
    11161 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
    11221 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
    11281 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
    11341 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
    11401 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
    11461 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
    11521 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
    11581 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
    11641 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
    11701 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
    11761 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
    11821 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
    11881 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
    11941 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
    12001 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
    12061 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
    12121 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
    12181 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    12241 aatggttcgt tggttcatct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    12301 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    12361 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    12421 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    12481 tgtctggcgt accttcgcta atgaaaatct ctagctcgcg catccgctct tccatttgtc
    12541 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    12601 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    12661 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    12721 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    12781 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    12841 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    12901 ttatactgcg agatttgcag aaatgataaa taaatgaata aagaataaaa tttcacaata
    12961 tctgagcaat ggaattgatt ttttcctata aatactgcta tgatggagaa atcaaagatg
    13021 taattagaca atacgtggag gtcatttcgt gaaagatcat attattccaa aggcgacagc
    13081 taaaagactg cctttgtatt atcgctattt aaggatttta aataatgctg gaaagaaaaa
    13141 agtgtcttca actgaattaa gtgaagccgt tcaagtagat agtgcaacga tccgtcgaga
    13201 tttttcttat tttggcgaat taggaaaacg tgggtatggt tatgatgtag aagatcttat
    13261 gcactttttt gcaaagacat taaacgacga tcaattgaca aatgttgcgc tgatcggtgt
    13321 agggaacttg ggaagtgctt tactaaaata caaattccat caaagtaata gcatacgtat
    13381 cagttgtgct tttgatgtga atgaggaaat cgttgggcgc atcgtagacg gtatccccgt
    13441 ttatccaatg gaagatatgt cagaacaaat caagatccaa caaatcgaag tagcaatttt
    13501 aacgataccc gctgaaagag cacaagaagt agtcaatcag ttaacggaag caggaatcaa
    13561 aggaatcttg aactttaccg ctgctcgatt gaatgctccg tcagatgtat tgatccaaaa
    13621 tgttgatttg acgaatgaat tgcaaacctt gatctatttc ttgcattctg acgcaaatgc
    13681 gcattccgaa aatacagcag aataaaaata aaaagcgatt gaacaaatga aggcaaaact
    13741 ttcacttgtt caatcgcttt tttctgtttg gacaacgatt attttgttcg gaagatttct
    13801 tcataagaaa gggaatcatc ttctgctgtc agttcatcta gtagataacg gattttttta
    13861 aagtcgatga accgtgcttg ccgaaagact tcttttcctt gatgaaaaat caggacaact
    13921 ggcgcagtca gcacttcaaa tttactagct acttctggtg tttcaaatgc atccaaatga
    13981 agtgccggaa tatcataatg tgtgagaagt tcctctagtc gtggacgaac agcgtgacaa
    14041 acactgcaat taggcatcga aagataaatg acggcaacgg atgactgttg cagtatgtct
    14101 tgaacttcag tcaagttagt ggtttgtttc atttttattc tcccttttat gatagaagtg
    14161 atatgtatga gaccttaatt taatgggaat aacagaaaaa gtcaattgaa gtgctttggt
    14221 aaaaaataag agttgtgtaa aaatttacag agcaacttgt tttttttgaa taaaagcgta
    14281 tatttgcaaa gcgaatgaac tagaaataaa aaggaggaaa ctcatgtttt cttttattcc
    14341 aaattcggtt ttgcagatga gtacgatttt cttatcgatc gttattgaag cattaccttt
    14401 cgtattatta ggctgtctta tctcaggcgc cttacaagta tttttgaccc cagaacgtgt
    14461 gaagagatgg cttcctgaaa atcgtatctt ctctattttt actggttcga tcttaggatt
    14521 tttctttcct tcatgtgaat gcggcatcgt accgatcgtt catcaatttg ttaaaaaagg
    14581 cgtaccagtt cacacagcgt ttgcttttat gctgactgct ccgatcatca atccaatcgt
    14641 gattttttcc acttttatcg catttggcaa ttcttggcag atggcaagct ggcgggtgat
    14701 aggcagcttt tttgttgccc ttgtgattgg tgtctggctt gcctattttc aaaaagacgc
    14761 tgtattgaaa gaaaaaatca cgtctaaagt agcagaaaat caccatcatc atgtccatga
    14821 agaagaacaa agtgagaaga atgaaccaaa aaatcagctg atccatcaaa ctgggcatgt
    14881 gttgacacat tcaatcgacg aattttttga tacaggtcgt tatttgatca ttggcggatt
    14941 aatcgcagct gcgatgcaga cttatttacc aactagaatt atgctaacgc tcggctcaac
    15001 aaagatttta gccattatca tcatgctgtt attggccttt acgatgtctc tttgctcaga
    15061 agcagatgcg tttataggat cttctttgtt aagtctgttc ggcacagcac ctgtagctgc
    15121 ctttttggtc tttggcccaa tggtcgatat caaaaatcta ctgatgatga aacgatattt
    15181 tacaggacgc tttattttat cgctgatcgg actgattgcc cttagcgtta ttggattcac
    15241 attatttatt taggagggaa aatatgctgc ggtttttgat tttatctggg tatttcgaat
    15301 tgatgatgta cttgcaagtt tctggcaaac tcgatcaata tatcaatgtc cattatcgct
    15361 acttagctat tctttcaatg attttatctg ctcttttggc tttagttcag ctgtatttgt
    15421 gggtcaaaaa cggaaaagca gaagaaaagg cacacgagca cgatcatgac catgggttgt
    15481 caaaacctta tcaacgtgga atcgcttatg tactgctagc tttgccagta atcgtgggga
    15541 cgttgtttcc aacagttagt ctcgacacta cgatcgtaga agccaaagga tttaatttcc
    15601 cagtaagtaa agaatcagta ggggatccgg agatgcagac acaatactta aagccggata
    15661 ccagtattta tttcaacaaa tcagactact tagaccaaat gaatgagttg aaagaaaagt
    15721 atggggataa gcaaactatc aagatcacag atgaaaacta tttagaagtt atggagctaa
    15781 tttataatta ccctagcgaa ttcattggca aaaaaatcag ctatgaagga tttgtttacc
    15841 aaacaccgaa cgatagcaat acagatatct tcttgttccg attcggcatt atccattgtg
    15901 tggcagattc aggagtcttt ggtttgttga cgcatatgcc ggaaggagta acggttaaaa
    15961 atgatgaatg gtataaaata gaaggaacca tccaatcaga ctattatcaa ccattcaagc
    16021 gtgaaatccc atctgtagtt gttacaaacg ctgagaaagt ctctgctccg aaaaatcagt
    16081 atgtgtatcg ttctttttaa aagaacaaaa taaataaaca tagctcagac agctgacacg
    16141 caaggacgtc ggttgtctgg ctttttttgc atgatgtatt catttgtttg atatttattt
    16201 tagaagcagc atcctgaaag tatttgatat cggtttcaaa ggtgttggaa aagattgtga
    16261 agagaattac aatttattcc taacatgtat gttgaaaagt tgtatgaaat gattttgata
    16321 gtaaaatatc gagtggaggt actttttatg gtaacgcgta tatttatttt ggatgatagt
    16381 ttggtttttg aaaaaatgct agtagaaatt ttggaagaat ccagaatttt attaagtcct
    16441 tggaaatttg agatcgttca gggattgaac gttatacaat ttgttgaatt cattaaaaat
    16501 aatgaaatct attcctcaga tatttttttc ctagacatcg atttgaacgc ctattatagc
    16561 ggtatcgaac taggcgaaat gcttagagaa aagaatgaaa agtgtaaaat tgtttatcta
    16621 acgtctcatc cgaataaagc aataactgtt attaatcgaa agattacgcc tagtggttat
    16681 ttgataaaag aattcactta tgaaaaaaat aaaaaaagca tccaaagtta cttattgaaa
    16741 gaaaaagaaa actatcctgt tcaacatgct acgaaaaaaa ggtggacaac tgtccgttat
    16801 ggtaatagcg atcactatat ccattacgat gaaatccttt atctaatgag catgccaggt
    16861 tataaaaata aaataggcct agtattgaaa gatgaagaga ttctcgtaaa cggcacaatt
    16921 aaaaaatata aaaaatcact cgaatatgag tatttatgta aagatttaaa agcttatatc
    16981 attaatactg aaaacattac atctattagc agagtaaacg aaatagtaac atttgcaaat
    17041 ggagaacaac tagagatggg aaagagaagt attgataaac taaagaattt tatgcaagaa
    17101 aagatagaaa atgaatcatt ttaaagaaaa caaagactca tatcttacct gttagaagag
    17161 tgttttcctg tagaagagaa caacccaagt agcagcctag tcgtttctat ccttttttgt
    17221 tatgcactct ttttgacatt tactccacca ataacgaagg gtacaataaa aagtaataac
    17281 aaacaaaagg agtacagaaa tgattgaatt catcaatgta agtaaaagtt acgggaaaaa
    17341 acaagcatta aaagacttgt ctctctcgat aagacaaggg gaaatttttg gatttttagg
    17401 acataatggt gccggaaaat caactacgat taaaagttta gttagtatta ttgaaccatc
    17461 atcgggaacg attttagtag atggaatgaa attgacggaa aatcgattga gtatcaaaca
    17521 aaaaatcggt tatgtgcctg attccccaga tattttttta caattgacgg ctggagaata
    17581 ttgggacttg atttcagctg cctatgaatt agacacacaa aaaaaagaaa agcgtttagc
    17641 agaattgaca gcgctttttg atatgtatag tcatcaaaat gaaacaatcg caagtttttc
    17701 acatggaatg cgtcaaaaaa cgattttgat cggcacgttg ctgccagatc cagatatttg
    17761 ggtattggat gaacctttgc aaggacttga tcctcaagca gcatttgatt tgaaagaaat
    17821 gatgaaggct catgccgcaa aaggaaaaac agtcattttt tctacacatg tattggatac
    17881 cgcacaacag ttatgcgata aactggctat tttgaagaaa ggtgaattga tttatcaggg
    17941 ggcagttagt gaattattga atggctcacc tgatgaaacg ctagaaaaga tttatttgaa
    18001 aatggctgga aggcaagcga caggagatgg accgtatgta taagcgacaa ttattagaat
    18061 taattcgagt taatctttta tatgttaatc cacagctcac gaataaagca agagagaaag
    18121 gaaaaaaggg aaagcgatta actatctatc ttttgtctca aaatcttttt tcagggctga
    18181 tttttttact gatttacgga tttgctatgt ttgctatgaa ctttgttaaa ttgccaggtt
    18241 tctttactta ttacgtggca ctttttggta tgttaggttt aactcaaagt atatcagtca
    18301 tttataatat cttttttgaa ggaaatgacc tgcaagtatt tttgcctctg ccttttggac
    18361 aagaacaggt tttttcagca aagattttgg tagttgcttt gaccatcatt ccttttgttt
    18421 ttccgcttct agtcatgttt attttaaccg gatggcgagc tgatctaggg attttgctta
    18481 ctatcattat ctcaatcctt ttgttttctc tatttttgat tattcttttt tgtctggcta
    18541 gtttgattgt ttttggtttg gctcgaacag cagtgtttaa aaaacataga aaaacagttt
    18601 ctagtatctt gcttggcagc tcgatgctga tcgcatttgc tggaatcatg tggatgaatc
    18661 atcaaacggg gactattgaa aatgagttaa cagatcggca tccgatttcg atattattgc
    18721 cattctatga tgctgccagt cagccacttt ctcaacaagg tttattgggt tggggaatac
    18781 tagctatcac ggctctggga tttttccttg ctgtaaaaca gctgatagta ccaaagctta
    18841 cagagcaact gactgattca aacagtgagt tcaaaataaa aagaacccat aaaaagaacc
    18901 aaaatgtgca tcagctttta tttagttaca atgctcaatt agtaaaagaa cctaatttga
    18961 ttatgcaagt tctttcttct tccttgttaa ctccaatcat ttttattctt gcttttgccc
    19021 taggaggcga aataaaatta actgatttag ataataagtt aatcggtgtt gttttcctgg
    19081 tagggatcgc attggctttc ttaacagtta atcagacttc ttttatcagc aatcttattt
    19141 ctttggatca agaaaatttt ctgtttatcc gttctttgcc aatctctatg aaccaatatt
    19201 taaaagaaaa gttcagattc ggatggatat tgcagagttt tttaactggt gggatcgcat
    19261 tattaagcgg actgtctttt cagctgcctt tgttttttat ccctagcttg ctatttggtt
    19321 ctttattcgg ctgctactta ttgtcactac gttatttcgc tagagattat cggttgctgc
    19381 tgctgaactg gaccaatatt aaccaattat ttaacagagg ttcaggaagt ttgggtttag
    19441 tcgcaactat gatgggttca ttgatcatca gtattattct tatcgtctta tacggaatgt
    19501 tggctttgtt ttatccattt tggttagtga atattcctgt gatcggttta gttctaatac
    19561 tttccgctct atggatcgtt tattatcaac gaacgttttg gaaaaaattt gaataaagaa
    19621 gaggggccgt gacaaaaatc atgtcgcagc ccctttttta cagatagagg gtgttcaaaa
    19681 gtcagtttct cagtattaag tcaattttat gtaaaaatgt gagaagctat ttttacaaaa
    19741 ctttttttaa tactcataat tacttcttat tgctcctgcg gttacttgtc gcaatgtttg
    19801 tgagatattt tgtcatcgct caaggctgaa cgtctctttc acaacctttt ctttgttaca
    19861 cttcgtttat ttaaaaagcc gtttttttcg tttcatcatg attgcgtcac gctttacttt
    19921 gaaattacgg atgttatggt taaagacttc tgataataca agacctaaag cgattgctcc
    19981 agcaatcata attgcttgga ccgcataatg agccgcttct cgataggatt cagtaacgag
    20041 atttcgaacc gcttgataag ctaaaccacc agggactaga ggaaccatcc cgggaatatt
    20101 gaaaatcgtt acgggcattt tcaaacgttt agagaaaaga tcgctgatga atgctactcc
    20161 taaagaacca agcaatgaac caaaagcagc agatccgcct atttgaacac agatccagta
    20221 gatcatccag ccaaaagcac cagaaagacc acaacaaatc aatgaacgcc taggtacatt
    20281 tgtaataacg gcaaatgccg cagtcgctaa aaaactaaaa gaaaactgaa taaatatcgt
    20341 ccccatactt gtgttccttt cctaataaaa aagctggaaa ataaaagcaa tcgcaaaacc
    20401 gatcatcgta gcagtcatca tcgcttctgt tccgcgagaa actcctgaga cataatgtcc
    20461 agctagcaaa tcacggatag cgttcgtaat ttgaactcca ggaactaaag gcatcacaca
    20521 tccgataatg attagatcct gattgcttcc taagccaaaa cgtacgctgt agatagcggc
    20581 tatgccaatc aataaagaag ataaaaattc cgataaaaat ttgattctaa aaaatttcaa
    20641 actatataga taagctgcgt atcccaatcc tccaatgact aaagtcacag gtaaatcggt
    20701 catctttccg ccaaataaca ccataattgt tccactgatc actgccgcgc taacgatttg
    20761 caaccagaca ggaaagaaag aacggtctcg gtcgattgct tgcagttcgc agtaaagttc
    20821 ttctaatgtt agctctgaag caacatattt acgtgaaaga tcatttactt ttactacttt
    20881 ttctaaatta atcgaacggt tggtaatttg ttccattcgg atggtcgagg tacgatcaag
    20941 accaataaag agaccggttt gagtgacata gctgaccaaa cggaaatttc cagaagctaa
    21001 agcaattcga ctcatcgtat cttctacccg atacatctcc gcattgcttt ccatcatgat
    21061 tttcccagcg agtaaacaag tgtccagcaa cagatctaat tcttttcccg tcatagcatc
    21121 ttctccaata attgattgat tagttttatt ttactacaaa acataccaga agcgtaaaga
    21181 ataaaaaaac ttgaggaagg aataaaagag gcgtatactg tttttgtatt gcgggtgtag
    21241 tttagtggta aaatcccagc ttcccaagct gatgtcgcgg gttcgattcc cgtcacccgc
    21301 ttaaaatagg ttgtgaaaaa gctgctctgc aacaagtaat aagaacagcc aagcaaaaac
    21361 aacttcttat gtttttaact tggatggact tattaccgca gatgcaagct tttgcgcacc
    21421 gtttaccaag cggttgtgaa atcagtgtat tgtcttgagt atgaagaaaa tttttgaata
    21481 ataataaata ccttacagat agagtggtga ctgattagag acgaggaatc cgtctctttt
    21541 ttgtattttt aagccatctt aaaacaggtt ttattttttg ctgtttctat ttctttctag
    21601 tatttattta taggggaaca tatgttaaga tttagggagc tgttagtaaa ggagtaaaga
    21661 aatgagcaaa aaaacaacca aggcgatcgt ggggatcgtc gctgctgtga ttgcaggggc
    21721 attcggtgtt tccaccacac aaaaagattc agatatcttt cagcagatta caactatttt
    21781 tgattccaca ccacaagctt ctcaagtagc ggatgtgcca gaatatgatg gaaaacatca
    21841 agaaatagaa atcaatggga atcagccgga atttacaaaa gaggatctta gcctttccaa
    21901 aggaacatgg gaaagctatt caaatattga taaattgaac agagtaggac aagccaatgc
    21961 tatgcttgga aaagatttgt tccctactga aaagagagag cctttgtata ttgacccgac
    22021 aggttggaaa caaaagaaat taagtgacgg gcaatggtta tataatcgca gccatttaat
    22081 tggtttccag ctgactggtc aaaataacaa cattaaaaac ttgatgacag ggacacgttc
    22141 gttgaatgct ccatacatgc ttgctcacga aaatgatatt gcggcatata tcaaagaaac
    22201 aaatcatcat gtacgctacc gtgtcactcc tcattttgag gggaatgaac tggtagccag
    22261 gggagtgcaa ttggaagcag aaagcattga agacaaaaaa attgaattca atgtctttat
    22321 ctacaatgtt caagatggct acacgatcaa ttatgagaca ggacaagcaa caaaaacaaa
    22381 aaattagtag cagctattcg aattttttat gaatattatg aaaaaattgt gtaaaaacat
    22441 acattcaaag aaatatagcg tatactttat gaaaagagta cttttgataa agctacagaa
    22501 aaaaggaaaa aaacatacaa atagtgtttc ttaatttatt cggaagtttg gaatatttgt
    22561 ttgtagtttt ccctttcttc gagtaaaata aggttgtaga aggtaagctg gaattacctt
    22621 gcgggaaaga ggtgtagaat catgggtttt acagatgaaa ccgtacgttt tggttttgac
    22681 gatagtcgca aaaaagaagt gagcgaaaca ttagcgattg tttatcgggc tttggaagaa
    22741 aaaggctaca atccaatcaa tcagattgtc ggctacttgc tttctggaga cccggcctac
    22801 attcctcgtt atcgtgatgc tcgaaatttg attcgccgtc acgaacgcga tgagatcatg
    22861 gaagtaattg tcaaggacta cctctcaaat catggagtga atttatgaga gtaatgggct
    22921 tggatgtcgg ttctaagaca gtaggtgttg ctatcagtga cccctttggc tggacagctc
    22981 aaggagtaga gatcattcgg atcaatgaag cagaagaaga gtttggcttt gatcggttga
    23041 aggaattagt agcacaatat gaagtagacc ggtttgtagt tggattacct aaaaatatga
    23101 acaattccat cggaccaagg gcagaagctt ccatgtcata cggagaaaag atcaaagaac
    23161 tattctcctt gccagttgat tatcaagatg aacgattaac cactgttcag gcagaacgta
    23221 tgctagtaga gcaggcaaat acttcaagag ccaaacgaaa aaaagtcatc gataaattag
    23281 cagctgtgat gatcttacaa aattatttag acggctcagg taagcaatta ttttaataat
    23341 aaaagaaaag aggtaatctt atgacagaac ataaccattc ccacgaccat gaaggacacg
    23401 aacacatcac attggtggat gaccaaggaa atgaaacatt atatgaaatc ctactaacag
    23461 ttgacggaca agaagaattt ggacgtaact atgttctttt atatcctgct ggtgtttcag
    23521 aagatgaaga tgtagaatta caagcttatg catacatcga aaacgaagat ggaacagaag
    23581 gcgagttaga acaaatcgaa acagacgcag aatgggacat gattgaagaa gtattcaata
    23641 cattcatggc ggaagaagaa gaatagacta taaagctagg agaaaccttg atatgatagg
    23701 gtttctcctt tttttatttt ttactgtatg ccatattgta tgccatctag tcaatattta
    23761 agtgttgtac taatttttca acagtctttt ctttcgtttc ttttgccaag tgaccgtaac
    23821 aatcgatcgt ctgactaatt tttgtatgcc gtaatctagt ttgaacttct accatagtag
    23881 ctccagcgtc caagagcatt gaagcatgag tgtgtcttgc tacatgcaca ccgagaaatg
    23941 gaaccatagc tttttcacaa atagtaatca atcttgcata aatagcgtgg tgtgtgatta
    24001 gttctttata gataccacaa aaaatcatag tcggatcagt tacaccaagt gtcagcatat
    24061 atttccgctg attaatctgc cattttttta atatagaaat tgttctttca tctagtggaa
    24121 gaaccccagc actacaatca gttttaggat cactgatttt atagccattt ttggattggc
    24181 ttagtgtttt attgatggtg accgtatttt cttgaaaatc aatgtctgac caattcaatg
    24241 caagggcttc actgatccta accccgctat aaaagagaaa gcgtagtaac gttttatcaa
    24301 agtcgtttcg ataagtttca ggtttgctgt ttaggtaatc aaacaaggtg atcagttgtt
    24361 ctttactgta aactttgacc ttttctttat gcttagtggt ggttctttta ggcattaata
    24421 cgttgtctag tggattagtc tcaataatcc caagattgat agcgtagcta aaaatacgct
    24481 tagaagttga atgaagtacc ttgtattggt cagtagtttc agcccagcta tttacaattt
    24541 gttgggcttt ttttaccgtg acttcattca cacgaagatt tccatatttt ggaagaatcc
    24601 actttctaaa cttgatttca gttttcatga aggtggcttc tcggacagtg gttttatatg
    24661 tttctagcca caagtcataa acttctttaa acgtttcgtt gttcgctttt ttaataccgt
    24721 ttttatcgta gtccacttgc aagcgtgaca aggcaagctg ggcttctttt tttgttttgt
    24781 aacctcgtct acgaacctta tcaggtttgc cagtcatttt gttcattcca agataggctt
    24841 catagagcca aaatttacct tttttagttt catattgctt gaatgttgcc ataatagttt
    24901 tccctccata gtgtagcaag ccgtgggggc ggttggagga ggcagtttat tattattttg
    24961 aatagtgttt atttagctca gtaacaagag ctgtgaactc ttttagaaga tcattttcat
    25021 aaaattcttg atactctttt ttctcatctg tatttttttc tgaatcatta attaatgagt
    25081 catatcgtaa aagcgtattt agaagagctg cgagataagc aattggggta ctcccgtcag
    25141 gaaattcaat tcgagatcct gttttaagat attttattgc gtttgttaaa taaatcttct
    25201 gtgagacagt caggttatct tcttcgatgt tattgatagc attagaaaga ttagaatcaa
    25261 ttatagagtt atttttttca atagtttttt tcaattgctt ttcaagcaca tttcgcttaa
    25321 tttcgggcat catatgcata tctcttaatg tataagatcc gtttaataga taggtagtgg
    25381 atacatctaa tacctttgaa agttgtttaa gcctggtgtt attgggaaga ttatagccat
    25441 tttcccaatt actaacggca cctttactgg ccggtgggga taatttacct ccaagttgtt
    25501 ccattgagag tcccttttca attctagcca gtctaattct ttgtcctgtc tccactgctt
    25561 cggggtattt ttttctagcc acattttcac caccattcat atattttctc tactatatca
    25621 cgatttaaat atgtgtgaaa cataaaagtt caaaaaagta tgttaaaatt cttgcaaatg
    25681 aaaaggtatg atggtatatt gagtttataa gaggtataga aatgtattca agagcctctt
    25741 gcattttttt aaaatctttg tatagaaatg tatgctaacg aggggggata aatatggaat
    25801 taaaaattga caaactcacg cttgatgatc cagcttttaa aattattgac gatcgagttg
    25861 ttcaacaagt agagcaaact caaggaacgt tactttcatt attagaaaaa aagtaccagt
    25921 ttccaaaata tatgaacaag gcacaagctg ctaagtatat gaacgtttca tacaatacta
    25981 tgatgcagaa atatgttcct aacggtttaa agttgattat agttgatgga gtcgtaagaa
    26041 tttcacaaga tgaatgtgat cgctttatgg aagaatacca aaaataaacg caagccgtgg
    26101 gggcaaaggg attaatagga ggaaataata atggaatcga cacaaaataa agcaatcgaa
    26161 aaagtattat caacaattct tacagaagac acagcggcag aattagcaaa cttggaaggc
    26221 aaagcgctag aagaaacgtt tgaatgtttg tacgaacaaa tggactatca aaaattgcta
    26281 ccacaagaac caacggtaag cggagtattg cgtggcttga acgatttagt acaagcagag
    26341 tttaaagaac gactttctat tgaagagtat caagaaattt tgtatcagca agttgagttg
    26401 ttagcgagct tgctagggct tgaactggag gacgttgaat gaaaactgaa atttggaacg
    26461 gacacattat aagatttgtc gatattaatg atgaatggtg ggcagtggcg aaagatgttg
    26521 cggaagcgtt aggtcttaaa caggttacta gagccattca ctctttgcct aaagatgggg
    26581 ttactactag taaggtcatc gattcattgg gcagaacaca agatgtaaac atcatcaacg
    26641 aaaaaaaata tttaccgcat ggcattcaaa agccgtaaaa aagaagccga ggcatttcaa
    26701 gactggattt tcgacatcat caaagaacta cgccagtcca caggacttga aggctttcaa
    26761 gtattccgca tgctagacaa agaacaccaa aaagaagcaa tgactaaatt aagccacgct
    26821 attactgaac caaaaccagt agactacatt aaagcgaatg tgatagccaa taaagctgta
    26881 tcaactattt atggtcattc caaaatggta aaaaagaaag atatgactcc tgaaatgttg
    26941 gttgatcgtg aacctatttt agatgagaca gtagaattaa tgactgttaa agaaaaatat
    27001 gggttgcagt tttcagtgag tgagaaaatt tataatcgtt ctgctgaatt gcagacaact
    27061 taggagcatt aacatgatcg agaacaaaga aaaagcctta tccataacag tcttggcgga
    27121 caggataaag gcaacttaat tgggataaaa cccgatattc tttgttccga ttataccata
    27181 ttaaaaaatc ggagggaacc attatgaaac gaaacttaat tataggaagc attttaatcg
    27241 gcggaatttt cggcttatta ccattaattg caaaaatagc tatctttgca ggattagcgt
    27301 tatatctctt cactgcttat gacgagtacg actatcaatt aagaactgga gggcataagt
    27361 agatgtacaa aacaaaacta ttaaaccaac tcgatagcct tgaattagag gaaattaatc
    27421 aagggatcgc tgaactagaa aataatatcg ggaaaaccta ctttggaaat tcattcaatg
    27481 aaaaactaac agttttgtat gtactcaaaa aacatgcaga acacaaaata atttgtcgag
    27541 aaatcaacga gctaaaaaat cagattttaa ccgcatggtt aaacattact gatatgcaag
    27601 aagcaagggt gaaaacattt aatacatggg taaagtatca gaatcaatta aaaggtgctg
    27661 aatttgttcg ggatgggttg aaatacgaac ttgagcagtt gaagctcatg gaggtgtctg
    27721 aatgatttat accagtattt tgaaaaagga ggagaggttt attggcaaat aattatcaag
    27781 cagcaacgga attacaaaag tcgggatggt caatttatcc cttatctcct ggaactaaaa
    27841 ttccaataaa aggctctagt ggttggaatg atgcaactaa cgattctaat caaattaatg
    27901 aatggtggac ggaagatgca actagaaata ttgggttaat gcttgaagat catcaaatgg
    27961 tggtagttga cctagatcaa cattctgaaa cacgaaacgg cgttgagaat tataaaaaac
    28021 ttgcttcaat gtatgatcca tttccttcta cttatacgga aatcacacca ggtaaaggaa
    28081 tccatttctt ctttaaaatt cctgatggtg taattatcca acaagaaaca ggtgcatttg
    28141 ctgatatctt cgggcgtgat gataaaggta aaagtttaag cgggattgat attatcacta
    28201 aaggagttcc aatagcgcca actatgatta tttcaagaag tgttgggaga gcatatgaac
    28261 ctattgaaaa cgtatctcta aataatattg caccagctcc cgattggcta gtgaacctac
    28321 tgctaaaaaa accgcaagaa ataaagactc aataccgacc cgtagcaaaa acagggacag
    28381 caaaaaaatt agatatgatt gttcaaggcg ctggcgaagg tagtagaaac gatcatttaa
    28441 ctaagttatg cggttggtta ctatggcacg gtgtagataa tcaaacttta gcagaattga
    28501 tttatacagc aaatgcgtac aatgaaccac cgctaaaaga taaagaagtg aatcaaatta
    28561 ttcgatcgat gattaaaaaa gatttgaggg ggaaagctca tggctcataa gcataaatta
    28621 gatagtattt tagactttcc cgaagccagc gagcgtgaag acaatatcat tgaattgaaa
    28681 acctggatgt cacgattaag atgcaataaa gacgatcaga ttaaaagtaa cagtgttgtg
    28741 aatgcagaat tgattttaac caatgatagc aatttagctg gaacaattgc ttataacgaa
    28801 tttagcggat acattcacct attaaaagat tctccttgga tcaatcgtac cgctggagaa
    28861 tgggaagaca gttttgagga tgccttaacg gcatatattg aagaaaacta caatgtagtt
    28921 tttgatgaca acaaaataca caaagcagta gtaaatgtgg ctagaaagaa tgttttcaac
    28981 ccagtaaaag aacgtatcga aaaagttaaa tgggatcaac agcctagact ggaaacactg
    29041 tttattgacc ttttaggggt agacgacaac ctttacacac gagaagtaac aaaacgttgg
    29101 atagtcggat cagtcgcacg tatctataaa ccaggaataa agtttgagat cgttccagtt
    29161 ttggatggac cgcaagggat tggtaagagt accgttccag cattacttta cacggatgat
    29221 tttttcaccg attcattaga ttcgctaggt gaaaagaaag atgattatat gcagcttcaa
    29281 ggcaatgtaa ttattgaact tggagaatta tcttcaatga acaaaactaa aattgaacag
    29341 ataaaaaact tcatcagtgc caagatcgat aaaattagac ctccttatgg tcggaatgtc
    29401 atagcttggg caaggcaatg tgtatttatt ggaacgtcga atgatgggca gtatttgaaa
    29461 gatgatacag gaaaccgccg tttttatcct ttgccttgta agaataagcc aaagatgaat
    29521 ccgtttaaaa caaaggatga atattttctt caagtgttag ctgaagcaaa ggtgcttttt
    29581 gataaaggac aacgaatcta ttttagtccc gatgaagata aagaagtctt agaaattgca
    29641 aaagactatc aagaggatgc caagatggag aatccaatta aagaagcaat cactaaatat
    29701 ttggaaatgg aaatcccgta taaatgggaa gaagctccag cgtgggcgag acgttcttat
    29761 tatcagaact atcctgacag taaatgtaat gagaaaaatt tacagcattt ctataatgga
    29821 gctactatgc aacaagagtt ttatttagtt gatagcgtac tcaccgctga tattttggaa
    29881 gctgtttttg ataaacaagc aaaggattta ttgaatggtc gatcagatgc agaaactaaa
    29941 aagattgcct tgattattag cagtattcct ggttgggaac ggaagcaact gccaaaaaga
    30001 aataaacgac gaggatttta caataagtca aatgcctatg aaaataaaaa tcgagctgga
    30061 aagtacaaaa agtaacgggt agtaacgggt cagtaacacc tagcccgtta ctagtttacc
    30121 cttactgccc caagggatac agcaatttgg taacggggta acaccttatt tccttaaaac
    30181 ttttgaaata gttagctaat atgctgggga ataatgttac tggattttac ccgttacctg
    30241 ttaccaaaac gctagaaccc ttgtccccgt tgggattgag tggtaacggg tacctgttac
    30301 gtaggtgtta ccacgtgtta cgaatcaagg aggaaagtaa atgctcaata ttatttcaac
    30361 aaacaaagca ccgaactttc aatatacaga tgaaatggat cggtttctaa tgaacacgct
    30421 ggcatttagt gtgggattgg taacagaaga ttattccaca tttgatccag aagtattaaa
    30481 gataatggaa gaagaacctg attggctgca ggagtcagta gcgtggtgtc aatcgctagt
    30541 agtgggatca ttggtagata gtggcaacta tgatgataca ggtgagctaa tggatgaatt
    30601 taattgtctg cttaacctat atgatcgagc gagacaacga gagcttacat cgaatgagga
    30661 taatttgttt ttaaacatcc atgataagtt cttggctttg ctactaacag atgatgaatt
    30721 gataactaat ttattggagg tagaataatt atgcatatgg aaagtattag atacttcgat
    30781 ggtgagaaac acattgagta ttcaaacact caagaagatg tagactttat tagttttgag
    30841 gaagataaaa tcactaaagt taattttaaa gacggaacgt gcttgaagat agtttcgcct
    30901 tatattgaat acaaatcaaa atgggaacaa gatgattctg acgaacgaga tcgctttggt
    30961 tggtaatcta tggcagttaa aaaacaatgt aatcatgctg gatgtaaaac attgattgat
    31021 tataggcaga agtattgtgg gaagcataaa gcaaagcaaa cggcaattaa gcgtgaagaa
    31081 agaaagcaaa gcgaaggtaa atattttcaa ttctatcaaa gtagaacatg gcgaaaggct
    31141 tcatacttgt atcggttaaa tcatccagta tgtgaggatt gcctagaaga aggcttgata
    31201 aggaaagctg atgttgtaga tcacaaaatt gaactaaaag atgattggaa tagacggtta
    31261 gatgaaacta attttcgttc tttatgccat gcacaccata attccaagac agccaatgaa
    31321 agacaaagac gagaaaagag taccctttga gttaaggggg catagtgtga aacttactga
    31381 caatcgatgc ctactcattt tggtacaaat aaccgttgtg aaaaggcata agggtaatta
    31441 caaataacga ttacacttgt aatcacaagt gaaaaagtat ataatgaaag taggagattt
    31501 attgaaaaat acaattgtta ttactgataa aacagggaaa cagcgcacca ttgagttacc
    31561 tcattttggt caagtgacca ttcaaatgca gaacggaaaa atcatttata tagataaatt
    31621 agataaagaa aaattctgat cgaaaaacga aggaatgtga gcttaattgc ttacgttcct
    31681 ttttcttttg tctgaaagga ggacaacatg ggacacccta aaatattaca cgatacaaaa
    31741 ggcaatatat caagtgaaga aaaagcaatc cgtgtggacg ctagagaaga attgttcaag
    31801 cagcaaccat taataaatat cacgccccct gactggatgg cagcgagcgc tagaagtgaa
    31861 tggaatcgta tcgtaccaac attaaaaaaa gattatccat tgagtgaagc agattatggt
    31921 tcattggtag cctattgttt agcctttgct cgaatgaaaa cagccgaagc cgagataaga
    31981 aaatcaggta cgtttatcac atgcgaaaac ggagtaaaga aagccaatcc agcggttcgg
    32041 gttcaatcac aagctatgag tgatttgaaa aaacaagcca cctcactagg tatgacctta
    32101 gaatcacgat caaaactagc tttgaacaag gttaaaaatg atgaacccga agacccgttc
    32161 aaagagttga tgaattcatg aatgattaca ttgagaaagt cctatcaggt aagctgattg
    32221 cacccaagaa aattatccaa gcgtgtgagc gtcatataag cgatttggag cgttctaagt
    32281 cagatagctt cccttatgtg ttcgatgaag aacaagccac caaagcgatt aaattcattg
    32341 agttgctacc atctacggac ggtaaagcaa tcaagatgtt aggatttcaa aaattcattc
    32401 taggcagtct ttatggctgg cgtactaaag aagggaatta caggcgattc aatcgagcgt
    32461 ttaccagtat gagccgtaag aatgggaaaa cgtatatcgc aagtggcatg gctgccaatg
    32521 cgttgattat ggaacaagaa ccagcagaag caaggcaagt attgtttgta agtaacgctt
    32581 tgaaacaagc taaattgggc tatgatatgc tgtctaattc acttagaaac gtggtcaagt
    32641 ctagtaagtt tttaagacca caactgaaaa ttatgaactc taagattcag cacttgccct
    32701 ctaattcgtt cgctatggca ctggctagtg aaaccagcac gctagatggg tttgcaccaa
    32761 caaccgcaat tcttgacgag tggcacgaag caaaaactcg taaaacgtac aacgtcatta
    32821 agtcaggaat gacccaacaa aagaacggct tattgtgtgt tattagtacc gctgggcttg
    32881 atttaaacgt tcctatgtac gaggaatact tattgttaga tcgtgtgcta aaaggcgaag
    32941 aacaagccga caggtacttt atagcgatat gggaattgga cgatcccgaa gaaattcacg
    33001 atcaagagaa atggatcaaa gccaatccga tttttgaaag tgaagaaatc aaaaaagtaa
    33061 tgattccaac cattcaagat gatgtgaacc ttgctttgaa acaaaataac cttaattctg
    33121 tattggtgaa aaacttcaat ttatggagac aagcgagtga ggacagctat atgattgctg
    33181 aagactggca ggcaaccgaa gtagaaccac aagatattac aggcaagccc gtttatatcg
    33241 gagtggattt atctaaaaca gatgatttaa ctagcgtttc atggatcgta ccgctagata
    33301 acggcgaact ttattgtgat tcacatagct ttgtagccac caaatatggg cttcaagaca
    33361 aagaaaagcg cgacggtttg ccttatcgag aactagaaaa agctggtgag tgttccatta
    33421 ctcaattaga aagtggaatc gtggactatg accaagtatt tcagtttatt caagatttga
    33481 ttcaagaaaa tgatttggaa tgtatgggga tttgttatga cccgtataac gctaattcgc
    33541 ttatcagtaa agctgaaaaa gccaactacc caatgttaga agtaagacaa ggaacgatta
    33601 ctctaaacgt tccgacccga acttttagag aacaagttta tgaaggcaac gttattcaca
    33661 ataaaaatac gattctcacc catgcagtga acaacgctat tttaaaaacg gataacaacg
    33721 gcattcagat taataaatca aagaacagta acaaaattga tccaatagct gcattaatca
    33781 atgcctatgt gtttgcaatg gattacttca ccacaacgga aggagcgaaa gcagacaatg
    33841 aattttatac aagtgaagaa ttttctttct aattacattc ataccgttct tttacttctt
    33901 ggattggtgt gtgtgttggt tgctatcacc ttactaacaa atgtctacta tggcttgtta
    33961 gcgctgggca tagtgcttat tggggtagcg gtcatgctaa atacagaaca gaagggaggt
    34021 taaaagatgg cattttttaa agcgagacaa aatacaacgg gagatccttt cctacaacat
    34081 gtggtatcaa tccaatcgga tgattacacc accagcttta caagcgttcg tgcattaaga
    34141 aatagtgatg tgtttgcagc cgttcgaatt attgctagtg atattgcttc aagtccgatt
    34201 caattggtca aaaacaatat gccacaagct gataatgaac tggtgaagtt actaaacgag
    34261 aaacctaatt cagaaatgga tggttggcat ttaaagtttg ctttggcggt caatatgttg
    34321 ctaaacggta atagctttgc agagattaaa cgcaacggtg aaaaggtaga agaaattcac
    34381 ttattaccta actcaagtgt aacggttact caattagata atggtacgtt gtcttatcag
    34441 attggtgata aaaaaagacg tgtgaagtct agcgatattt tgcacttcaa atatttcacc
    34501 caagatggtt taacaggatt accaccactt tatgcgttac gtgatgaact gaaaattcaa
    34561 caagctggca accgcacatt gcacaatttc tttactcgtg gtgtcagtgg gtcaggtatt
    34621 ttgaaggttc ataagtcaga tttagacgga tcagcaaaga acgcaattcg tgaaaagttt
    34681 gaagaagcca acggttcaag tagtggagat aatgctctta gaacgatcat tcttgatgaa
    34741 acaatggact acaaaacatt agaaataaac accgatgttt tgaagcttat caattccaat
    34801 gattggaaca caaaacaaat tgccaaggcg tttggtgtac cgattgagcg tttaggcgtt
    34861 gaaaatgaac attctagcac cgttcaaagt aatctccagt atatccaaag tacgctgatt
    34921 cattacttta atgtgtttgt gagtgaatta gatacgaaac ttgaaaccaa tatccgcttt
    34981 aattccgatc agttactaga gactgatcct gaaacaaaag taaaaaatgt attggatcag
    35041 gtcaaagggt cacttctcac gattaatgag gggcggtcga aaatggggct accccccatg
    35101 gatggtggcg atcgtttact agcaagtttg aactttacgt atttagatac gttggagaaa
    35161 tatcaattaa aagaacagga aggagttaca ccagttgaat aatgaagaag aaaaggaaaa
    35221 acggctgaca gaagaagctg agctaacagc cgattctccc aaagtagaga aagaaaatga
    35281 aaaacaacca acagacggca aaactatttc aggctatgcg ttgaaattcg ggcaaccgtc
    35341 aaaagattta ggcggctttg tagaagtgat tacacccgaa gcattaaaag aggtggattt
    35401 atcaaatgtg ttcttattat atggacacga ttattcaaag ccactagcta gtgtcaaagc
    35461 aggtacgttg aaattgaatg tcgatgatac aggattacat tttgaagcag agctaaccga
    35521 cacgacctat tcaaatgatg tgtatgagaa tatctcaaaa ggggttgttg attctatgtc
    35581 ctttgggttc gtgcttggat tagattcatt cgacaaaaaa gaagatggca caattgaacg
    35641 atcaatagat aaaatcaaag cacttaatga aattagtgtc gtgaccgttc ccgcttatga
    35701 ttcatcaaat gtccaagtaa ataagcgttc ttacgaatcg tttatgagta acaaccaagc
    35761 aaaacaaaca aacaatagct tagaatccac ttctaaagca caaaaggaga gtaataacat
    35821 ggaaaaaact ttaatcgata acgaaaaaac agaattgcgt gggtatgaag aatatatccg
    35881 ttcacaaggc gaagtgcgtg atggagtcac tactgtaaat gcagcggcag ttgttcccga
    35941 agaagtaatc ggtgaagtct ttgatttgaa acgttcaaat tataacttag ctcaatatgc
    36001 aacagtaaaa acagtatcaa atggacaagg taaatatcca gtagcaacta accaacaagc
    36061 agtgttagca acaaaagctg aacttgctga aattggtgat attgacgctg aaatgtttac
    36121 ttcagttgat tataaagtag aaactcgtgc tggtaagatt gccttatcaa atgaggttgt
    36181 ggaagattca gcagtgaata ttgtacaaga ggtcaaagat cagttagcga aattggtaga
    36241 aaacactgac aataagcata tcatggattt attaaaaaca ttcactaaga aaacggctgc
    36301 tacgttggac gatttgaaac aactatacaa tgtggcatta gacccagcat taaataaaat
    36361 ggtaattcta aaccaaagcg gatattacca cctagataca ttgaaagatt cagatggacg
    36421 ttacatttta caacccgatg tgacagcacc tagtggtaaa tcattattcg gtatgccagt
    36481 agtattgatt gcagatacat tgtttgccaa tcctaaagct ggaacattcc ctatgattat
    36541 gggggatatt gcacaatcta tctttgttgc tcgtagaaat caagtaacga ctcaatggga
    36601 aaaattcgat tactactcac aaggacttgc agtgatcgtt cgcaacgatt ataagaaaat
    36661 tgatcaaaat gcttcagtgt atattgagtt tacgccagtt gtaacaccaa aagtatagaa
    36721 aaatattggg cggcggttta tcccactgcc tttttttcat taggagagat aaaatggtaa
    36781 ttttggatag cataaaaaaa agtatgcgga tcgatcacac tattgatgat gactttattc
    36841 aacaattgat tgatacagca ggcgaatata taaaaagtgc tattgatagt agcgcaacgg
    36901 ataaagatat ggataattat cagcaatttg atttggcggt gtcattactt actcaacatt
    36961 ggtatttgaa ccgtcaagaa gccagcagcg aacggatacc agtaacggta caagcattag
    37021 tacaacaaat gagaggtgct tattatgcca atcattaaga atgtaaatga attgactgag
    37081 agagtcaagt ttaaaaagac aaaacgggta aaagatgaag atgggcagat ggtggatagt
    37141 gaagaaactg tatttgaatg ttgggctaat gtgcgttcac aaatgctaaa agacgtgctt
    37201 gctagtgtag gtaccattct tgaaggaacg ttgacgttta ttattcgata cgatcaagat
    37261 tatgaactaa caaacgatat gaaagtagct tggaaaaaca agagttataa aattatttca
    37321 atcaatgaag gaacggcgtt taaagattat acaacgatca tagctaaaca gatttcttag
    37381 aatgattaca cttgtaaacg ttgtgggtat ttagtataat taaggtagta aatgaagggt
    37441 tagctgcctg aattttgcaa gacttagcta gtcgaaatct attgttggac tgaaaattgt
    37501 aggtgtggtt gcaaacatgc tggactaaga aagatgatag atgatgaacg ttcaatttta
    37561 atacaggcat gctaggactg attggatggt tttggcaagc tgattgtgta gaaatcaaag
    37621 aacacgtctg ttgtggacgt gttttttttg ttataattca aaagaggtga tttaatggac
    37681 aaagcagagt ttacaaattt ttattggaag tattatctac atttagaaaa tgagtttatt
    37741 aataccacag attttgtgat gctcgatgag aataatttca aaacattctc tattgaatat
    37801 caaaaactac tactagcgat tggttcggag tgtgagatta ttttcaaaga actttgcgga
    37861 tttgatagca gtagcaataa aagcataact gattttaaag ctgttattaa atctagcgaa
    37921 ttaatgtgtt tggataatgg tgttagggtt atgaattctt taacattgac ttcactaaaa
    37981 ccttttggag atgggtggcc tgaagataca ccagattggt ggaaaatata taacagagta
    38041 aaacatggtc gttcttcaaa ttataaggaa gcaaatttgg aaaatgtgtt gtatgcttta
    38101 gcaagtctat atttattaga ggaatatttg cataagaaag taatactaga aggtgaagta
    38161 gactttataa gtccagagtc aaatctattt acgttatctt gggaaagaaa acattcgtcg
    38221 atgcaaaaag tgacgtttga aaaagtggat tcaaattatg tagctccact cattgatttt
    38281 aaagaacaag aagaaattta aaaaccccca cggcttattg aagtttttgt atgccatatt
    38341 gtatgccatt tggtttaaat cgcttgaaaa atagtgatag ataactctaa ttcatttagt
    38401 cataaacgct gttttgataa cctttatagt tctttgatat gggtttctca ttcatggcgg
    38461 aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg attgtgacaa
    38521 acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc ccctcggcat
    38581 tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc taataatcag
    38641 gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca actcctcggt
    38701 cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact
    38761 cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
    38821 cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat
    38881 tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa
    38941 aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt
    39001 aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat
    39061 tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg
    39121 ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac
    39181 tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg
    39241 aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta
    39301 gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag
    39361 ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat
    39421 catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttggtca attgttacaa tgcctttgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaagagta tactatgata
    39901 aaaaaattca ttttaaaaaa tcagaaattg gctggtgatt tggatgttac gggaaatgcg
    39961 tttagaagat gcagaagcac tacgtgaaat caatgcggat caattagggt atgatatacc
    40021 attaaccgtt acggtgagac aaatgaagaa actgctgaag gagccagaga aaaatttttt
    40081 cttggtttat gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta
    40141 tgaatcgatt tttagcgatc caatgttcaa tattatggca cttgctgtgt ctaaatatgc
    40201 tgaaaaaaga ggcatcggaa aggctttgat gactggcgta gaagaagaag cgaaaaaacg
    40261 aggaataacg acaataaggc ttaattctgc agaatacaga gtagaagcac accaatttta
    40321 tgaacacatc ggttatcatt ccgataaaat gcaaaaaaga tttttgaaaa tcctgtaaat
    40381 ctttatcaaa aaaatacccg atcaccaagc cgatacttga tgatcaggta tttttgtttt
    40441 tttgactaag aaagtaattg catcaagaaa ccagagatga tcaaaacgat ggcaccacca
    40501 agtcgattgc ccatttgagc aaaagcaata agttccatcc gattagctgc agataagacg
    40561 gctacatttc ctgttccgcc catcgagttg ttgcataagc cagcagtaat agttgattca
    40621 attggatata aaccaaacaa acgaccgaca aatcctgaaa caactgaaat cgcgataaca
    40681 cttgtcagac ataagacaac aaattgccat gtcaaagcag aagctaatac attcagattc
    40741 agcaacgcaa taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagtta
    40801 ttgaacatga tagctgcatc ttcataatac tctggtaata ggttggcgat tttacagatg
    40861 acaacgataa taatcataaa tgcgtaagca tggactttag gaacaaaata gttaccaatt
    40921 tgtcctaaga tgaagaaagt caatgatacc atcaaaccaa cacctaactg tggaatggaa
    40981 ggtttcacag ttcgaggttt tccatcgcca agatcgcctt tttccattaa tttaccattg
    41041 ccgttgaatt ttggcattga ttgtccagca cgagcaacta atgctgcacc gatgatagct
    41101 aagacatttg tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcatccgta
    41161 cctaaaacat ttgcataaat cgttgataaa ggaacggctc cggctccaac accaccagcc
    41221 atcgcaggta atgagatgta taaaacggaa tttgcaaaac catttccgat aagcatgccc
    41281 acgagtccta cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga
    41341 acagaagctt tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca
    41401 gcaatataaa agtcccaaaa gcccatattg ctgacaaagt tttctgtgga tttcactaca
    41461 tattcaggta agatcccaaa tgtagcaatc gcagcagaag cgaagatcgc aaaaacggaa
    41521 ccaccaccca aataactttt gacgataggg atttttgtcc cgataaaatg aaataaatta
    41581 ccgaaaataa ccaaaactgc gattggtcca atcatattcg tcggtaattt ccctaaagcc
    41641 attgcaatga tcaggacaag gaccatgatc aaataaagtg gcaaacttac gccggtgatt
    41701 tttgtggacc aaaaaccttc ttttttcttt tcctcaactg cagttgcagt tgttgttttt
    41761 tgtgccattt ttacagatcc tttcttattc tgtatcatat gaaaaaagcc aaagaaaagc
    41821 gacaaaagag caggagttgg ctggccgctg ttttgccgca gtcgcttttt tctttttaga
    41881 cgtcatacaa agtttctgta tgttctttta ctataacgat cttaatcttc taaagattta
    41941 tattcaggaa cccatttagc agattcaact gcttctttga tatccgtgat ttcttcacga
    42001 ttcaagtttt gtgcgacaac tgattttgcg acagtttcag caatcgtttg agaaaattca
    42061 gtgatttttg ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg
    42121 cctaatgctc gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca
    42181 atcaaaccaa gtcctaaacc tgggtacatc aaagcattat ttgcttgacc gatttggtaa
    42241 gttacaccat tatattcaac atcagcagca gggatacctg ttccaactaa tgcttttccg
    42301 tccgtccatt ctattaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa
    42361 ggcatgatga tcggacgtgg tgtatgagca gccatttctt tcacgatttc ttcagtgaat
    42421 gctccaggtt gggtagaagt accaatcatg attgtcggat ggatcgcttt tacaactgct
    42481 tctaagtttg tcaattcatc agcattagtg aaatctgagc gtttgcgagc gaatgggatt
    42541 tgaccaggag tcaatccttc tgtatcttca aacaataaac cttgtttatc tacttgatag
    42601 aaatgttggc gtgcttcttc ttcaggtaca ccttgacgga tcatctcatc taaaaggatg
    42661 tttgcgatac ccacaccagc agtaccagca ccaaatgtca aaattatttg atctttcaat
    42721 tgttcaccgg atatattcaa tccgcctaat acacctgcta ggacaacaat tcctgtacct
    42781 tggatatcgt cgttgaaagt agtgatttga tcttcgtatt tttctaagat cgctgcagca
    42841 tttcctcgtc caaaatcttc ccaatgcaat aacaattctg ggaataattc ggtagctgat
    42901 tgaacgaatt gatcgatgaa ttcatagtaa gtttcgcctt ctacacgagc atgacgattg
    42961 cctaagtata agggattatt caataattct tcattattag taccagcgtc gatcgataca
    43021 ggcaaaactt gtgctggatt aataccagca gcagcagtat agaccatcaa tttgccgata
    43081 gcgatgtcaa caccgttcac accccagtcg cccataccta agattccttc tgcatcagtc
    43141 acaacaatca aacgaatgtc tcgtccatct gccgcgttct tcaaacttgc tttgatgttt
    43201 tctggttcgt cgattgaaag aaatgcagca tcttgcggtt tcaagaaaat ttcgttgtat
    43261 tgttcgattg aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt
    43321 tcgcccatca atttataaaa caatgtccga ttcgtattga atagattcat taagaagata
    43381 cgtttttcca aatctgtttg tttgcttaag tattgaccat aagcttgaac agattgttgt
    43441 tccagtgttt ggacagtact tggaagcata ccagtgattc catattttgc ccgttcttct
    43501 tttgtaaaag ctgttccttt atttagaaaa ggattattta ataagtttaa ccctgtaagc
    43561 atgaatgatt cctcctaagt tttaatacaa acgaagtata gaagtcagat gatgatatag
    43621 tcaaaaagag caggtatgat aaattttatt tatatgtaat atagccaaaa gaaatcaaaa
    43681 tagcagtatt tgttcttttt atatcgacaa tccttgcttg aatactatgc gtatctctta
    43741 tatgctagag aaagaaaagt agaagaataa gggatagatg gaatggataa ataggatttc
    43801 tgaggaaaaa agcacatgat agcgttgata ggagtcacct atgtcaggtg ataaaaatac
    43861 ataagtagaa aattaagagg aggaagagaa aatgaaagaa gttcaattga ttagaaaaag
    43921 cgaactatca gaaggcggtt gcaatgcatg tggcgtagta gaagcgacaa gctatacatt
    43981 aaaattagat tccaatcaag caataatctc tgaactgaca gtaggcggtc ttgtggacag
    44041 tctggcgctt gctgaaggtt ttactggaga agatatttat gagatgtttt cagaagtccg
    44101 tcagttaaaa aaaggagaaa aatgcataga ggttcatcat gagagcccga atgtgaggtt
    44161 caaacgtggt gataatgaga tgatcttcaa gaatcatgtg tctgatcaca ctgaactata
    44221 tgaaatcgtg aatcaaatac tgtctgggat ttttgaatta ggaccttatg agttcaagga
    44281 agaaaacggt aatccaaaat taaatgaaga atggcaagaa acgattgaaa cacaaagaaa
    44341 caatccacac ttatttcaat agtcttccac agtatttcgt aaaaatgttg atgaaaaaga
    44401 aacaccgtga atcagaggtc gtgaaaaacc tgccctgcat caagtaataa gaacagccaa
    44461 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagtttt
    44521 tgaacaccgt ttaaggaaga gactgtgaag tcaacgttct gacctgagta ttagagagac
    44581 aaatggcaaa atagttcctc atattttgcc attttttgag ctaataccga gggtcagctg
    44641 attgaacacc gttttatcga gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
    44701 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac cgagaaaatg
    44761 gcttttgaac accgtttatt caaaaaaagg gactttgaca ctacttttgt caaagtccca
    44821 tcggtttttt atctattttt attacaatca tttcttccag agtagtgaca tctggaatgg
    44881 ctgaaatgta aacagattat ttgcctgtat caatgtcgaa gagtaatata aaaagtgtaa
    44941 acgctatcat attgttcatc attcaaattt tatagagaga aggcattgag atggaaaaaa
    45001 catatcaagc aggtacaaat gaaggaatag tagattttat caatatggaa gatttagaaa
    45061 tcgcggcttc acaagtgatt ccagctggtg gttatggtta catcagcagt ggtgctggcg
    45121 acttatttac ttatcaagaa aatgaacggg cattcaatca tcgtttgatt attcctcatg
    45181 tattaagaga tgtagaatta cccgatacaa ctacacattt tgacgaagaa atgctgactg
    45241 caccaatcat catggcacca gttgctgctc atggattagc tcatgtgaag gcggagaaag
    45301 cttctgcaaa aggtgtagca gattttggga cgatctatac agcaagttct tacgcttctt
    45361 gtactttaga agagataaga gaagcaggcg gggagaaagc accgcaatgg tttcaatttt
    45421 atatgagcaa agataatgga atcaatttgg atattcttga agtggccaag cgtaatggag
    45481 caaaagcaat cgtattgaca gcggatgcaa cagttggcgg aaaccgtgaa acagaccgca
    45541 gaaacgggtt taccttcccg ctgccaatgc cgatcgttca ggcttatcaa tcaggagtag
    45601 gccaaacgat ggatgctgta tataaatctt ccaaacaaaa acttagtcca aaagatgtag
    45661 aatttatcgc tgctcattct gatttgccag tctacgtgaa aggtgttcaa tcagaagaag
    45721 atgtctatcg atcattagaa tctggtgctg gtggaatttg ggtatctaac catggtggcc
    45781 gtcaattaga tggggggcct gctgcgtttg attcgttgca atatgtggca gaagcagtag
    45841 ataaacgtgt accaatcgtg tttgacagcg gtgtacgacg tggacaacat gttttcaaag
    45901 caatcgcatc aggtgccgat cttgttgcta ttggcagacc cgttatttat ggattgtctt
    45961 taggaggaag tacaggagtc catcaagtgt ttgatttctt taaaacagaa ttggaaatgg
    46021 tcatgcaatt agctggaaca caaacagtag aagatatcaa aaaaataaaa ttgcgtgaga
    46081 atcgttttat ttaagaaata aatgacgaaa aaaataggag tgcggcagat gatgccgtac
    46141 tcctattttt atagttcaag gatgtcacta atagaataat ttgaaacgaa aaaataaaat
    46201 tgaaatattt tttcatactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    46261 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    46321 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    46381 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    46441 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    46501 aataccgttg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    46561 gaaaatccag caactggcca gacaggttct ttaacattgg atgtagttcc tgaattgcca
    46621 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    46681 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    46741 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    46801 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    46861 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    46921 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    46981 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    47041 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    47101 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    47161 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    47221 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    47281 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    47341 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    47401 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    47461 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    47521 cacatgcaga aacaactata cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    47581 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    47641 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    47701 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    47761 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    47821 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgaatttt
    47881 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    47941 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    48001 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    48061 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    48121 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    48181 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    48241 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    48301 tatgatctaa gtccagttcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    48361 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    48421 gaaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    48481 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    48541 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    48601 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    48661 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    48721 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    48781 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    48841 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    48901 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    48961 tgccggtgcc cccatcaagg ttactagcgg tagtttatta tcagcaggaa gccatacctt
    49021 gcgttttggg atggggtcgt gggatacaaa tactctattg gcagatatta ttgctattat
    49081 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    49141 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    49201 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    49261 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    49321 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    49381 agagataaat acgtggaata gttacatctc aacaagtgat tcaacaggaa aatataatac
    49441 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    49501 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    49561 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    49621 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    49681 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    49741 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    49801 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    49861 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    49921 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    49981 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    50041 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    50101 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    50161 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    50221 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    50281 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    50341 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    50401 cattcatctt acttacaact atcaattaaa tactgtagat acttctgtac aatccgtcag
    50461 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    50521 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    50581 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    50641 aagttattat gaagcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    50701 cacgtctcct tggcaactga cagggacatt gacttcttta tttaagaatg atcaaggtca
    50761 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaaacaat tgattcagca
    50821 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    50881 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    50941 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    51001 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    51061 aacttctgct gttaaaatca gaaatgcttg gaggagtgga cttggaactt ttcagtttgc
    51121 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    51181 agatttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    51241 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    51301 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    51361 gctacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    51421 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    51481 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    51541 ccggtccaga aagtcaaatt tgttattttt atttccaatt attttggttt ggacgtcctt
    51601 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    51661 aaaaagagtt tagttttact attacagaag aaggacgatt gaaatttgaa atatggcttt
    51721 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    51781 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    51841 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    51901 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    51961 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    52021 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    52081 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    52141 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    52201 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    52261 ttgaattatt tgctcgctat gcatctattt tgcggacgct gatcaagcag ttagaattac
    52321 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    52381 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    52441 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    52501 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    52561 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    52621 cattggtgct tcccatgcag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    52681 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    52741 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    52801 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    52861 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    52921 taaattaatc gtagcaacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    52981 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    53041 agcaaaagaa gtagtgatct tcggtgctgg ttatattgga ttggaattag cagatgcttt
    53101 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt taacaagata
    53161 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaatcaattt
    53221 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    53281 acttctatct caagcaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    53341 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    53401 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    53461 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    53521 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    53581 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    53641 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    53701 tgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    53761 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    53821 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    53881 acacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    53941 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtgggg
    54001 atataataaa tattaatcaa tcgagaataa aaaattcata taatgaaata gcggaagaat
    54061 tttttgagca taatattcgc caaaaattta tcaagtgata aaattgttgt ataaaatgat
    54121 tatttcagga ggaggagtta aatgttttta gcattaaatg aaattatgca ttcaaagtta
    54181 cgttatgcgt tagttgcagg ggtgatgttt ttgatcgcct acctggtatt ttttttgact
    54241 gggttagctt atggcttagc tcaagataat cgaacagcag tcgacaagtg ggaggcagat
    54301 tcgattgtgc tttcgaaaga tgcaaattca aatctaggga tgtccatgat tactaagaag
    54361 atagcagaag aagtcgaagg tgggaaagtg gcgtatcttg cccagacacc tggagtcgtg
    54421 actagtaaag acagtacaga agaaggaaaa atcaatgtta gcttcttcgg tatcgataaa
    54481 aatcaattca tcatgcccaa tcttgttgaa ggaaaagctt ttgataacga tgatgaggca
    54541 gtaggcgata ttagtttgaa agaagaatat ggtctagccg taggagatac agttaagtta
    54601 tctggtagtg acaagacgtt taaactaact ggctttacgg atcatgccaa attcaatgtc
    54661 tcaccagttc tctatacaac catcaatgct taccaagaga tccgcttcga aaaagaagat
    54721 acaagtgaaa atgcacggat taacgcaata gttgtcagag gtaaaatcag taatttacct
    54781 gaagatttag aacagatcaa aatttctaaa ttcatcaatg aattacctgg atataatgcg
    54841 caagtattga cttttggatt tatgatcgga tttttgatcg ttattgcggc tattgtgatc
    54901 gggattttca tttatgtact gacgatgcaa aagattaata tctttggtgt aatgaaagca
    54961 caagggatca caggaggatt tattgcgcgt tctgtagtgg cacaaacatt cattttatca
    55021 tttgttggta ttcttcttgg tctattagga acagtgggaa catcccttgt tctacctgat
    55081 gcggttccat tccaaagcaa ttggctgttt tttggcgtca ttagcttgtt gatgctggta
    55141 gtagctgtgt taggcgcact attttctgtt cggacaatcg taaaaatcga tccgttgaag
    55201 gcaattggat aagaaggagg aagagcatga aagcaataga atttaaagaa gtagaaaaaa
    55261 gtttccaaga tggcgaccaa gtagttaaag cattgaaaga aacaaccatg agtatcgata
    55321 aaggggaatt tgttgcaatc atcggtcctt ctggttcagg taaaagtact tttttgactt
    55381 tggcaggcgg attgcagacg cctagcagtg gctctgtatt tatcaatgat caagaattca
    55441 gtaatcaaaa agaaagtgaa cgcgttaaac tacgttttaa agaaattggc tttattttgc
    55501 aagcttccaa tctggttcct tttctgacag taaaaaagca gttattatta gtagataaga
    55561 tcaaacatga acatcgtcaa aaagaagcaa aagaattatt tgagcagctt ggcgtggata
    55621 agttgatcaa caagtatcca gaagaactat ctggtggaga acggcaaagg gtcgcaatcg
    55681 ctcgtgcatt gtacaatgat ccgtccatta ttctagcaga tgaaccaact gctagtttgg
    55741 attcagaaaa ggcacgagaa gtagtcgata tcttggcaaa agaatcaaaa gacaaaaata
    55801 aagcaacgat catggttact cacgatacac ggctgattga caaatgcgat agaatattta
    55861 tgatggaaga tggtgtattg aaagaaaaag aataaccaaa aagcaggtgg aaaaatggtc
    55921 catcgataca acaaagatac acagcaaaaa attttgaaag aagcgactga cttgtttatg
    55981 tccaagggat atctcggcac ttcaacaaga gagatcgcac aaaaagttgg gatcacccaa
    56041 cccaatctct atcattattt cggtgataaa gaaaaactat atacggcagt tttagaaaac
    56101 cacttaaaag atgttggaaa agctttgaga gaaatcgttc aaacaagcga aacgggattt
    56161 caagtaacct tgacaaaaat ggcgcagttt ttgattgaga cacatctggt cgatttgttt
    56221 ctgatgcttc acgatcttga aagcaatctt tcaaaggaaa caagagatca tcttttcttt
    56281 ctatggaaga aaaattatcg tgaacctttt gaagaaatct tttcagcaaa ccaatctgtg
    56341 ctaagaaatg ggatttcaaa agaaatcgca gcaaggcatt ttttcttagt gttagcacct
    56401 tacatcactc ggtcgaaaag acctgtggaa gaaccgctaa atgtggcgca attgatcgat
    56461 ttatatttga atggtgtatt aggagaagcg taaagctgtt atatagggag aaaaatagtt
    56521 aggtgaagaa gcaagatatg acgaagatcg ttatatcttg ttttttttat ataggtagtt
    56581 tttcataaat acaaatttgt tattctctca tgattatcag ggtatatttt tatttattat
    56641 gtcggtgttc caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga
    56701 aggaaactat ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata
    56761 tgccaaaaat gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta
    56821 cagccccttt tttgcgtact taagctgatt ttaagttttg acccttagtc tggatctacg
    56881 aggtgagagt caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt
    56941 attatctgtt ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc
    57001 agtacgaaca gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca
    57061 ttaatcaaaa tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca
    57121 cttgcaacta taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat
    57181 cagatcaaga caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc
    57241 acaaagaata tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct
    57301 gggcatggcg gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat
    57361 ataacattaa gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt
    57421 tatttgaccc atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa
    57481 gaaaaaaagg cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa
    57541 gcaactggaa tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt
    57601 gctgactcct tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa
    57661 gttcttcgag aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat
    57721 agtgatttaa aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca
    57781 caagcattga ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc
    57841 atatttttcg tttggccggg cttattatcg cagatgcaag cttttgaaca ccgtttaagg
    57901 aagaggttgt gagcctgccg tttaactccg cgtcacaagg agcaatttca aaaaacagct
    57961 tctcatgttt gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta
    58021 ttcggaacaa gggactgtga catgactttt gtcacagttc cattattttt ttagcagctt
    58081 caaagctttt tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc
    58141 gcctttctcc aatagttttg ccgctacttg gtcagatttc gttccagctc ctgatggaag
    58201 ggtgattcca gcttcagctg atgctttgtg taactcttcc aaaaagctgg cacgactgat
    58261 gatagaagca gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt
    58321 ttcttgtaat tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa
    58381 ctgatcgatc aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat
    58441 cgcttgattg tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt
    58501 gtatttttct ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc
    58561 aagctggatg atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa
    58621 ttctgcaagc tgatcacgtt tcacataagc agcacatact gttagcggac caaaatagct
    58681 gccatttccg acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga
    58741 agaggctgtt tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc
    58801 tattgctgca ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt
    58861 cccgtctttt tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg
    58921 atagtatgct ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat
    58981 aaaattctcc tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta
    59041 agagattgcg agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa
    59101 aatatttgtg gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc
    59161 aaataggagg aaatgattcg tatggcgcac gaaaaaacac gctacaaagc agtgattgcc
    59221 aaccaaacat atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag
    59281 ctgatcaatg aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa
    59341 gcagcaatct tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaatc
    59401 ttggagctgg aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt
    59461 gaaaatcgtg tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa
    59521 aatggtcaag cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag
    59581 aaacgaaaag gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga
    59641 aaaatttaaa aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt
    59701 ttattgctaa ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc
    59761 gcgattggat atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt
    59821 catttggaat tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt
    59881 gatcaggtat ttgcttttca cctagatgaa gctttttatg caggaactgc ttttctgatt
    59941 attttgctaa ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg
    60001 tatgtgccga tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg
    60061 gcttacgtta ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc
    60121 caaaatcaat tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt
    60181 accaacaaga tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg
    60241 aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg
    60301 gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa
    60361 acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga
    60421 aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt
    60481 caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa
    60541 cttgaaaata tccgtcccca tatgaaacgg atcgagatcg gagcagactt gaatggagtg
    60601 gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat
    60661 gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc
    60721 acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact
    60781 gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct
    60841 gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg
    60901 atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta
    60961 tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat tgaaccaaaa
    61021 caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt
    61081 caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac
    61141 aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa
    61201 gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga
    61261 catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat
    61321 caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta
    61381 ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa
    61441 atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt
    61501 ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa
    61561 aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct
    61621 ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg
    61681 cattatcctg aattgaaagt ttatggatac aatcgggcaa atacgattaa tgccagcatg
    61741 gagttcgatg tggatacact aagtccaacg tatcgtttgc tgattggcgt tcctggacga
    61801 agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg
    61861 aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat
    61921 cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa
    61981 gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa
    62041 atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga aaaagcagaa
    62101 aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag
    62161 catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag
    62221 aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg
    62281 ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa
    62341 gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct
    62401 caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg
    62461 ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa
    62521 tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga
    62581 acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc
    62641 tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa
    62701 agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta tactaatcat
    62761 agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg
    62821 aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc
    62881 gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa
    62941 ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca
    63001 ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt
    63061 caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa
    63121 gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta
    63181 ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga
    63241 catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa
    63301 aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga
    63361 acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact
    63421 ggcagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc
    63481 gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat
    63541 cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat
    63601 gaaaaatatc cccgtttggt gattactcgt aaagtactaa aagacaaagc actgtatttt
    63661 ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt
    63721 cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga
    63781 caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat
    63841 gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa
    63901 atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc
    63961 cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt actggatcga
    64021 gatgtatttg gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt tttcgtaaga
    64081 caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa
    64141 gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa
    64201 gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg
    64261 ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa gaatgcctca
    64321 gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca
    64381 gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat
    64441 aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa
    64501 ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat
    64561 gcatcaatga gagaagtcat ctatcgcaga tattcacgag tgattcgcga agggttgcct
    64621 ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc
    64681 ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa cgataaacat
    64741 aaaacaaatg agttgctatt cggtatagat ttggctgtag taccgcttga gcgaaattca
    64801 ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc
    64861 catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta
    64921 ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca
    64981 gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt
    65041 gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca
    65101 ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc
    65161 tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag
    65221 gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc
    65281 gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt
    65341 ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt
    65401 ggggagaaag attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa
    65461 gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag
    65521 attgaaccaa aaatcgattt ttggatggaa aaatttcagg taaaagggaa aaagacggat
    65581 aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc
    65641 catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct
    65701 gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct
    65761 agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga
    65821 aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta
    65881 ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg
    65941 atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa
    66001 atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact
    66061 ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac
    66121 caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc
    66181 ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt
    66241 caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa
    66301 aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc
    66361 ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat
    66421 gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac
    66481 tggcttgcaa tcgatgcttc gagcaagtca attaggcatt tcaccagaag aagtagctag
    66541 cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat
    66601 gacagttgga gaagataact cgacagtcca atatgtcgta agttatgtag caacgattgt
    66661 cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa
    66721 aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg
    66781 aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt
    66841 tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat
    66901 ctcattggat aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg
    66961 cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga
    67021 tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggatata tgcttggatt
    67081 gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt
    67141 cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta tgggtggagc
    67201 attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa
    67261 catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt cattgaaaca
    67321 atcgatttca attatgagaa atgaacgaaa aaaagcataa aatatagaaa aatatagaag
    67381 gaagctggac atttattgtt cccagcttct tttttgtata gacctatttc tattttttta
    67441 ttatattgga tatatatatt cattgaaaat agagcataac aactttataa tgttgagtat
    67501 accaattttt ggaggcgttt taatggaccg ctatttagat ttactttcag agaagttccc
    67561 tacaacggaa gatgtcatca cggaaatcat caatctcgaa gcaattttgc agcttccaaa
    67621 aggaacagag ttatttttga gcgatataca tggcgaattt cctgcttttg atcatattct
    67681 gcgtatcggt tcaggaaatc ttaaagaaaa gatcaaagac cttttctctg aacaaatgac
    67741 ggaaacggag atcagccaat tcactatttt tacggcttat ccagaatatg tgttgacgac
    67801 tgattggtac aaaaaacaag ataaatccca gctgatccat cgtttgatcg atcttttacg
    67861 gtttacgaca gtcaaataca ctcgatcaaa agttcgaaaa ggattgccga aagaatatag
    67921 ctatatcatt gaggaactct tgtatatcga tgatcgtatc aacgggaaaa aagactatgt
    67981 aaaaaaaatc attgaacaac ttgtcttgat gaaggaagaa gaaaaatttt taacaaaact
    68041 tgcgcagacg atccaaaaat ctgtcatcga tcatttgcat atcgtgggtg atatttttga
    68101 tcgaggaact caagcagcca aagtaatgga tcgaatcatg gatttttcct ctgtagatat
    68161 ccagtggggg aatcatgata ttttgtggat tggtgcgtat tgcggctctg aggcatgttt
    68221 attgacgctt ttacggattg ccgcacgcta caactattta tttgaattag agaaagaata
    68281 tggattaaat ttacgtccat tattttcttt tgcccatcaa acctatcaga agaatccagt
    68341 atttacccct aaaaacagga aagatctatc tgaaagagag caagaagaat tagaaaagat
    68401 ccatcaagca ctttctatcc tccaattcaa atcagaacat cagttgttga atcgacgacc
    68461 tgaatttaaa atggacgatc gtaaattgtt ggaaaaaatc aactatgagg aatcaacgat
    68521 cgttttagaa ggtcagctct atgagttgaa agggacttgt tttcaaacga tccagccaga
    68581 tgatcctaaa aaattcttgg aagaggaaca aaaagtgatc gatagtttga tgtattcatt
    68641 ccagcactcg ttaagaatgc aaaaacacat gacgtttttg atcgaaaaag ggggaatgta
    68701 tctcacgaac aatcagcatg tactgttcca tggctgtata cctgtagatg aaaaggggca
    68761 gctcttagca tttgaattag accaatctta tcgcggaaaa gaactgctgg gattttttga
    68821 aaaacagata actgaaagcg caaaagattt gactgcaaat gaagatttgg cgaccgattt
    68881 gatttggtat gcgtggagcg ggccattttc gcctttattc ggcaagagta aaatggcaac
    68941 ttttgagcgc tattttctca cagagcctca tacgcacgtc gaaaaaagca atccctatta
    69001 tcacttgcga gatgaagagt ggttcagtga aaaaattttg gcagaattcg atgcaaaaga
    69061 agaaggttca gcaatcatca atggacacac gccagtaaaa gtaaaaaaag gagagtcacc
    69121 gatcaaagca aacggaaaag catttgttat tgacggaggt ctatccaaag cttatcaaaa
    69181 aacaaccggg attgctggct actcattact atgcaattct tacggtttcc aaatcgtgac
    69241 acattatccg ttcttgccta ttgaacaaca atttgcaaaa aaatctgatc aaacgaatgt
    69301 aaaaaaagtg atcgagcagc agctcccacg caaacttaat cggaatacga caaaaggaat
    69361 ggaattacag caacagatta ctcaactcca aaggctgttg gattatcgaa aggacgattg
    69421 agaaaaatgc tagccaacat tgctcaatag actaaaaagc gactgcaaca aaaggcgtgt
    69481 cgcagtctcc ttcggaatta gctcaaaaaa cggtaaaata ttagaagcta tttttctgtt
    69541 tatttctcta atactcaggt cagggcgctg atttcacgac ctcttcctta aacggtgttc
    69601 tcctgccaac tcctcggtca caagtcacaa tatccaacaa acatgagaag ctgttttttg
    69661 aaattgctcc ttgtgactca gagttgaacg gcaggctcac gacctaatca gaaaaacgat
    69721 taaaattcat tcacaaagaa atcgaataaa cgctgttcag atgaatctac acgatgagaa
    69781 agttccggat gccattggac accaaggatt ttgtgcatat tatctttact ttggactgct
    69841 tcaatgagtc catcgttgga aaaggcgatg gcttttaaca aaggagaaag atctttgact
    69901 gcctgatgat gataggaatt cacaaagtat gaatcaggga gaaggtcact taataaactg
    69961 tccgaaacga ttttgacttc gtgagttgca aactgcgggg cagtgggctg ttgttcgtgt
    70021 tttacggacc attttggata taatgaaaga tcttgataaa gtgttccctc caacacgaca
    70081 ttcaatagtt gaagtcctcg gcagactgca aaaatcggtt ttctttgttt caatgcttcc
    70141 aagatcaatg cctgttcgaa ttggtctcgt tgaatatttg tttccgtcag ttttggatga
    70201 gggtcttcca tgtaaagttg cggactaaca tcctgaccgc ctgcaagaat gagtttgtcg
    70261 attttctcaa ttagttgagg cgctgaatct ggagacatga ctggcagaat aagaggtaat
    70321 ccttgagcat ctaaaactgc atccacaaat ccctgcggtg tatagcttac ttgatttcct
    70381 tggaaagtat ctgtcgctcg aatcagttga ttcccggcaa tgccgatgat tggtttcatt
    70441 tttattcccc ctaaagtaga ctaattgtaa caaggattta aaaaaattta aactatgtaa
    70501 gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag aattttcgtg
    70561 tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa agtaagcaga
    70621 aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga gaaggacatt
    70681 ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt caatgaaaaa
    70741 agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta gaagctttgg
    70801 aacaaatccg aaaagcagaa gaagaaaatg agaagcgtgc tcgaacccta cggcagcagt
    70861 tgaagaccta tgcagaagaa aaagagcaag aattggagat aatccaaacg aacagtcggc
    70921 aacagctgaa acagttggta gaagaaaaag aacaattcga aaagcaagaa ttagaaaaag
    70981 aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa gaccaatatc
    71041 aaaagcatca gagtcaagca atcggtgcaa tcattgaaag ggtgaaagaa acttatggcc
    71101 gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga gaggatttta
    71161 caagcagtac aaggaatcca aaatgttgaa atccgggatc tgtatcaaaa tagaacaaac
    71221 aaccagtggg tagaaaaata ttttccagat accacgatta ttgataaaga agcaaaatta
    71281 agcgctttag gcaatcggct gaaagacatc caagaagcta tccaattcat acaacaccac
    71341 ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt acaagacttg
    71401 gaagcaaatt attccgaaga aaatttcatg aataaattag aagaagtcct tgatttaaaa
    71461 gaaaagtggg aacagctaac tgaaaaacgt gatcgtttag aagaagagga agaatggctt
    71521 cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac gaccattttt
    71581 gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact aagtaagctt
    71641 gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct ttcgtatgtt
    71701 gctttaaaaa aatcagttgc tgctatagaa gaaattgctg ggaaatacgg gttcaataaa
    71761 gaaacgtatc cttatgaaca aaatcctaaa gaaaaattgc aagaaaccaa acagcaatta
    71821 tccattgttc acgagcagca aaaaaagata acgacagcgt taggtcagtg cagcggctat
    71881 atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg ggaaaaagta
    71941 aaagaacggt ttatccatgc cacttatctg atcgttttgc aaggttgggt agatgcagaa
    72001 gaaaaacaga atttgatgca tgtgttaaac gaaactgtat cagaagaaga tatatacgtt
    72061 agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa attgaaaaac
    72121 catccgctta tcgaaccttt tgaattgctg actgaaatgt acagtctgcc aaaatatgaa
    72181 gaagtagatc caacgccttg gatgacacca ttttatcttg tcttcttcgg gatgatggtc
    72241 gcagatgttg gttacggatt gctgatgctc attgggacaa tactagctca aaaactattt
    72301 gttttacccc gcgggatgaa acgtttcgta aaattctttg agattctctc ggtaccatcg
    72361 atcatctggg gattgatcta cagttcattt ttcggacagg cattaccaaa agaactattt
    72421 ggtatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac gattttgatt
    72481 ttatccgtga ttttcggtct gatacagatc ttggttggat tattcgtggc agcaaaggaa
    72541 aatatcagga ggaaagatta cttgagtgct gtcagtgatg gttttgcctg gcaagggatc
    72601 ctgattggga tcgttattgc attgattggt gtcatgttga taaaaaatac agcatttgtt
    72661 tatcttggcg cggggctatc gatcattgct gctttatgta tcttaatcgt gccgattatc
    72721 caatcgccgt ctaaagtcaa gggtgctgcc aaaggagcgt ataatcttta cggattgact
    72781 ggttatatcg gtgatttagt cagttataca cgtttgatgg cactaggtat ttcaggcgga
    72841 agtattggcg cggcattcaa tatgctagtt gctttcatgc cgccagccgc tcgattcagt
    72901 gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac actgttaagt
    72961 gcctatgtcc atggtgcacg attgcagtat gtcgaatttt ttggaaaatt ttatacagga
    73021 ggcggacgtg cctttgaccc gctgaaaacg gcggaaaaat acgtcaacat caatcataag
    73081 aaaaaaaaca ataaataatt ggaggatttt tatacaatga tggattactt aattagtcaa
    73141 aatggtggaa tggtatttgc agtattagcg atggcaacag cgacgatttt ttcagggatt
    73201 ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcgttaac aacaagtcaa
    73261 cccgaaaaat tcggacaggc tttgattctg caattgcttc caggtacgca agggttgtat
    73321 ggattcgtta tcgccttttt gatttttatc aacttaggaa atgatatgtc agttgtacaa
    73381 ggattgaact ttctaggcgc ttctctgccg attgctttta caggtttgtt ttcaggtatc
    73441 gcccaaggga aagttgcagc agcaggtatc caaattttag cgaaaaaacc tgaacatgct
    73501 acaaaaggaa tcatttttgc tgcgatggtt gaaacatatg cgattttagg tttcgttatt
    73561 tccttcctat tagtattgaa tgcctaagca taaaggagga aaagcagatg aacgccattg
    73621 aaaaaatcat ctctcagatg aatgaagcag cagagcaaga aagagcggcg ttagagcaag
    73681 aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaaatc gaaacagaac
    73741 atcaaaaaca aaaagaaaaa caaatcgaat tacttgaaaa aaaataccgt cagcttagga
    73801 atcgccaaca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa tttttacgac
    73861 gattgtttgc agatgcaata gctgaaatgg agaattggga tgaatctgaa caaatccagt
    73921 ttatcaaaaa ttcattgtat tcattacctt tgacaggcaa agtaacattc attgctggag
    73981 aaaaatcagc agcttatctt tctcagacat tgctggatga atggaacaat gaacttccct
    74041 ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg ataaacgatc
    74101 aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa ggaacgatga
    74161 gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg aataccacga
    74221 actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga catttgaacg
    74281 gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca cgatttacag
    74341 tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa aggagcagag
    74401 ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt ggatctatac
    74461 gatgcgctat actttccata atctgaaggt tttgacaaaa gccgagatga caaatcaaaa
    74521 tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga aggatgccat
    74581 tcatacgcaa gtttcaaatg aactccctga aagcattatg gacagtatac gagaagtacg
    74641 tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg atcgacagtt
    74701 tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc tgacagaagt
    74761 cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat tgcagaaaag
    74821 aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa aagaagtatt
    74881 tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag agacagatta
    74941 tgctgatttt ctgcgccctg tcatccgtga gggaaatctt gatctcgtaa agcttgaaca
    75001 gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg ctttcggacc
    75061 actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc ttcgtttact
    75121 ggttatcggc aaaagaagcc atttcgaacc agaaacaatc agaaagcggg tgagacaagt
    75181 ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca ttcaaattgt
    75241 ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca attgaagaaa
    75301 tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca ttgagcccag
    75361 aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt cccaatcatc
    75421 aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa gcagtaggtc
    75481 aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa atcataaaag
    75541 tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa gacatgtgtc
    75601 tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat gtggcgtcta
    75661 ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc tctactggtg
    75721 aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac ggaatccaac
    75781 ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc ggagttcaat
    75841 tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa gccggagaaa
    75901 cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata aaacataaaa
    75961 tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt tcattcacta
    76021 ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg acgatgcttc
    76081 aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg gatgcaccca
    76141 tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga ggagcagcag
    76201 ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt gcaaaatggg
    76261 ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag atgacggatg
    76321 ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg atggaacgta
    76381 cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct tctatctata
    76441 cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct atcatggcag
    76501 actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg gaagaaatgc
    76561 ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac tatgaacgat
    76621 ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc gcaatcagtg
    76681 ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca ttgcgggtcg
    76741 taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt ccttcgatcg
    76801 attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg gatcatatcc
    76861 ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag gaagaacagc
    76921 agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat gaccgtctga
    76981 cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct tttgatgatg
    77041 tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc ttgacctttg
    77101 gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc atggacggta
    77161 ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca gaattagaaa
    77221 aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt tcagaaggag
    77281 gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc attgatggcg
    77341 gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat gcaaaatggc
    77401 gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt ccaaatattc
    77461 gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg ccatccttta
    77521 gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg acaaccgaaa
    77581 gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga agtaatcaat
    77641 ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc aatcgatcat
    77701 ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg tcttcctcat
    77761 aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc agatgatttc
    77821 gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt catggaagat
    77881 tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc aaacgaccct
    77941 gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata tcttgcttat
    78001 gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc agaggcattg
    78061 cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc tgggtatctt
    78121 tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact aaaaggatcg
    78181 gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc aatccctgac
    78241 ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta caagagtggg
    78301 attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa gggaactgga
    78361 cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc agcctatgca
    78421 caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt atctgatgta
    78481 gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa tcaaggattc
    78541 tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct atcgatgttg
    78601 ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct gccagaaggg
    78661 aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt gactcgattg
    78721 aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa gcaagatgaa
    78781 ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca agaaatggaa
    78841 gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat cacggaacct
    78901 ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat cgtagacaaa
    78961 aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca gctgacagac
    79021 acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc aatcgatcgg
    79081 tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac ttgtcaactg
    79141 atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata tatgacgatc
    79201 cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa cgagcgcgca
    79261 gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt ggtatctttt
    79321 tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt ttgccggctg
    79381 ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc ctacttaccc
    79441 taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt ttcactgcaa
    79501 cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat tggaacagtg
    79561 ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt atgatggttc
    79621 cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt attatattaa
    79681 aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt tacatcctaa
    79741 aattggcctt tgttatccaa ttgattggta cggttgcttt atcttttgta tttgtgcctg
    79801 aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct agtttttgta
    79861 atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat gtttatttga
    79921 tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta tggcgtgatt
    79981 tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca ttaaccgtta
    80041 caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc aatggggcaa
    80101 cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg agtgtgacac
    80161 ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca ggtctgattt
    80221 taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc ggattgaaaa
    80281 caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga cgtacgagag
    80341 cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt actttatttt
    80401 ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact gaaacgatac
    80461 ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt ggtacagtag
    80521 gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc attatagcgc
    80581 ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg acaaaagcac
    80641 aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga taatttcatc
    80701 ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt tcttgcttct
    80761 tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt tgttgaacct
    80821 cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt tatttatcaa
    80881 taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca gaagctaaaa
    80941 tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc gcacgtgtag
    81001 taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc tcaattatct
    81061 tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga ttcgttggtt
    81121 tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga cttaatttag
    81181 tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact tccacatcta
    81241 atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt tggacatgta
    81301 ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc attccagcta
    81361 agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta tgttgatagt
    81421 aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct ttttcaatca
    81481 cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga ttattacaag
    81541 aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc aatatatcag
    81601 gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat aactgagcca
    81661 attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct ggagagaaaa
    81721 gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat agagattcta
    81781 aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat tgaaaatttg
    81841 tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta ctagttgatt
    81901 gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga ccaaaatcaa
    81961 tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca ttgggagaaa
    82021 taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca tatttataag
    82081 ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt tgggagggta
    82141 actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt tgaatcaacg
    82201 atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac gggtaattgt
    82261 cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac aattttacca
    82321 ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct atgaaaatta
    82381 ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa gtaatgaaaa
    82441 aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg gtggaaattt
    82501 tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg ggggagaaca
    82561 gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat catttacctt
    82621 ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa gcattgataa
    82681 acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca acagattact
    82741 tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa gcattgtcgc
    82801 atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac gatattttag
    82861 aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa ataacgttta
    82921 taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt acgttgacct
    82981 ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat atcaatgctg
    83041 tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa acttctcaaa
    83101 tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt atagccatgg
    83161 cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta gtttctgaga
    83221 aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt ggtctagcta
    83281 ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg gctttaaaat
    83341 gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag gaaatccagc
    83401 agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa ttgccattga
    83461 aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg agcttagatg
    83521 gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa cagcatttgt
    83581 tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc aaagcggtaa
    83641 tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta ttatgccgaa
    83701 aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta aagaagccta
    83761 cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta aattgcagcc
    83821 agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga cagcagaaag
    83881 tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca atgaagatcc
    83941 agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa tgaataaaac
    84001 aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg aattaaaaga
    84061 agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct cgtttatttg
    84121 atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat agctacaatg
    84181 gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc tcattttaat
    84241 tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac tatcctcgga
    84301 tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc gcagtgccag
    84361 gtaatccttt taagtcagta atctcaagac cggcagcttt atgttgctcg tgtaattttt
    84421 ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca tacgcaccat
    84481 gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc aaataaacaa
    84541 gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca aatgcctttt
    84601 gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga gccattttca
    84661 tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa ggaaatagtt
    84721 gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata aaactgttaa
    84781 ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt gctgattatc
    84841 aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca gtaatcgagg
    84901 ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag acgaaagaag
    84961 aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt tcttcttatc
    85021 tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat tataatccag
    85081 gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa aagaccaata
    85141 aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt agccaccgat
    85201 gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt agatcagctt
    85261 tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc tacaccgtgc
    85321 tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac ggcatttccc
    85381 attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt atctccgata
    85441 gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac tgccgcacct
    85501 ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac cgttgtgtat
    85561 ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc tggttcgtcg
    85621 atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt tcgatatttt
    85681 agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc tcgattaggc
    85741 gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg tgccatcact
    85801 tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt acctgatttc
    85861 gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg aaaaaggttc
    85921 aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa aacgatcgaa
    85981 acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac ttctcttttc
    86041 tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat aatttcctcc
    86101 aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc aaattcttga
    86161 taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat ttcttttgga
    86221 aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag ctgactaacc
    86281 tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt gcgatgtctg
    86341 ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac agcagtgtaa
    86401 taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac taattttgaa
    86461 tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa acgtttagct
    86521 aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt gcccaagacc
    86581 ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa tggcagaagt
    86641 aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg tgcgcgatga
    86701 agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta cacttgcaca
    86761 tacatttgat atcggctaac gatataatct tccactgcat gcatgccact catggaaaaa
    86821 gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc aaatgttcca
    86881 tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc tgcatcgatc
    86941 tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat cacactagct
    87001 accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc tgtttctgga
    87061 gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatatg ttcaaatgta
    87121 tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt caccagccgt
    87181 tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg agaaaagata
    87241 tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg gctatgttct
    87301 gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag acgttggact
    87361 tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata gttatggact
    87421 ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt tgtcataatg
    87481 tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc attgctccag
    87541 ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag aatacagctt
    87601 ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt ggtcaagcag
    87661 ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg taccaaagtc
    87721 ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccgcgttttt cctgattacc
    87781 gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct gagcgatacc
    87841 ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag aatgggtgat
    87901 ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga gcatttgctc
    87961 gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca aagaaatatt
    88021 caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca ctggagcgta
    88081 acctgcatct ttcagtgttt ggatcccgtc acttagaaaa ggagttcgaa catccttcat
    88141 acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct ggtgttcttt
    88201 agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt tttgataaat
    88261 gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata aattcatatt
    88321 ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt ttctgtaaaa
    88381 atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat gagaagtaaa
    88441 ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt gcgtacaaaa
    88501 gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca aatggtgaca
    88561 attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg cgatgagatt
    88621 cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc aggagaaatg
    88681 cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg aacaccatac
    88741 ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa agaccgtccc
    88801 acaggaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa aatcggcgaa
    88861 tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat tttttgtgtc
    88921 tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga aattagtgta
    88981 tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc gatcttagaa
    89041 caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa ctatcttggg
    89101 aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt gaatattgac
    89161 ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta tgctatcgat
    89221 tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac tccacgtcgc
    89281 cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat cgattacaat
    89341 gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct gtttgatgac
    89401 gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga aatcggagca
    89461 gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga tttaagtatc
    89521 atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga ctctgaaaaa
    89581 gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta tcagaataag
    89641 gaggtgtgac acgatttttg tcacaccttc ttttttgtat ataaaaatcc caagtaaata
    89701 cataaaaaat aaaaaaattg aaacttttag cgcaccttgt acgtcatatt aagtgaggca
    89761 gaaaggagtg gagaatgatt tttactggat ataagaaaaa acggcagatc gaaaaatacc
    89821 gtcacctgct gaatggactc atcgaaactg attatcaaaa aatgttccgt atcacgatga
    89881 attacgtaca tgataaagaa gaggccttag atgtgatgca agatagtttt cataaagccc
    89941 tgaactattt tgaaaaagaa aaagagatcg agcattttag tgcatggttt tatcggatat
    90001 tgatccgcac ggcattagat agttggcgca gacaaaaaag gacgcctgtg gacttatttg
    90061 aaattcaaga tatcccccaa cagttacctt tagaaacttc cgttgcagag ctccatatga
    90121 ttttagataa aatagacagt ccggaaaaag agattctgat cctctacttt tttgaaggat
    90181 ttcgtttgaa agaaattgcg actatcttgg atatgaatga aaacacagtt aaaacgaaaa
    90241 tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg aaggagagaa gagtgccatg
    90301 tattcccaaa aagaaaattt aaaaaaacta aagaaaactt ataaagaaca agcaattccc
    90361 aaagggctta aagaggagat ccgccagcgt tttattatcg aagagcaatc atttataaaa
    90421 gcaaagcgga gaaagagacg tctccaaggg ttcggagtat ctttggctat cgtaatgatt
    90481 acaacaagta gccttctttt taatagtcaa gttcgaagct ttgcagaaga tttacccatc
    90541 ttaggatcag tcattgaatt gatcttaggc gaacgcttta ctgatcgatc tgaaaaaatc
    90601 gatattcagg taccgaagat caatacacaa aatgagaaag aaaataaaac aattcatgga
    90661 ttaaatcaaa aatactttcg cgaaggacaa gcagattttg aaaaagctga aaaagaatat
    90721 ggaaattttg aaacggatca ttaccaagtt ttgggagatt atcagaaaat cgtagacgat
    90781 aaccggtttc tcgtgattga aaggcagatc acccaaacag cagcagatag ccatgttgaa
    90841 aaaagatacg acacaatcga caagaaaaac agtgtccagc tctcacttcc acttttattc
    90901 aaagatgaca cctatctatc tgttttgaca aaagaagtca aacggcagat ggccgaacaa
    90961 gtcaaggaag atccatctaa gtactattgg actgagcaag atattcaaga agggacgata
    91021 gaaaaaccaa cacttgttac gcctacacga agtttttatt tgaacaaaga gcatcaatta
    91081 gttttgactt tttctcaata cgaaattgct cctggttata tgggtacacc agaatttgtc
    91141 attcctaagt ccgtgacaaa aacgattttg gcatcggaag attatttgga tcattagacg
    91201 agaagtagtt gcgactagca gtttcaattt tcaagtaagt acaagtaaat aataagaaga
    91261 gaaaaagaga aagaagagat atagtgaaaa gaaattattt agttatcagt attattgctt
    91321 tatccgtgct tttgtttttc gaaattactt cggtggcctc cttgatcacc gaagtaaaag
    91381 gacaaacaca ctcttcggag cattcgtcac aatctttcaa acaatcagaa caatcaacca
    91441 ttaaaagcca atcaactgaa acagctaacg tcaaagaagc cattcaagaa tcagcggaaa
    91501 aggtacaaag tcagtcatca caatcaacac aagacagtac aaaaacgagc cgagatccgg
    91561 ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg agcgaccaca acaccgcagc
    91621 ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta ggagaaaata
    91681 cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc agggcacgag attggcaatc
    91741 acacatacga tcatcagaat ctggccactc tttctgccca gtcgatgacg gaggaagtga
    91801 cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac ccccactttt gttcgtccgc
    91861 cctatggttc tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc atcgaatggt
    91921 ctgttgattc agaagattgg aagacaagaa atcctgattt gattttgcag aagatccaag
    91981 caacagtata cgatggggca attattttat tccatgatat ctatcctgaa acgattcgtg
    92041 ccgttcctca agtgatcgat tatctgaaag agcaagggta tcgcatcacg acagttgggg
    92101 atttattagg gcatccaacg gctgtcgaga actattatgg cagaaacgat catcggccag
    92161 ttcagtaagt gagacaagta tctttaataa ataacacatt ttaataagta gttgaacaca
    92221 taactacctt acttgcaaag gcttaacaat atctatacag gtacaacaaa aaactgtcca
    92281 ccaattttct ttggcggaca gttttcactt aaaaataaag aagcaactat tcgtaaatta
    92341 tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca agaaccggaa
    92401 tcttacgtga tatccactac actaaggacg caagcactgg gataattgta actaaacaga
    92461 taaaaaatta caaggggatt ggacaaaaaa atgaaatttg agcaaaattt ttcacaaaaa
    92521 cagcagcaga cacagaaact agccatgacg caacagttgc aacagtccat ccaagtttta
    92581 caatataaca gtgaggagct gttggcattc gttgagaatc aagcaatgga aaatccattg
    92641 gtggaagttg ttgagccgga atggcagcca gattacataa aagcttcttc atcttcttat
    92701 gaaggagaag agacaaatta cctaaatcag attcctgata caaaagggtc tttatttgat
    92761 tcattgatcg aacaagtcca tttgaattac cgagatactt ttttgaggaa aatcgttctt
    92821 tatcttgtag aatacatcga tttgaacgga tttttgacga ttagtttaga agaagccgga
    92881 aaagagattg gagctacgcc tatccaaatg ctcgatgcgt tgactctgat tcagcaatta
    92941 gaaccagctg gagtaggtgc acgtagtttg caagaatgtc tcatgcttca aacagaacga
    93001 gatgattatg cgccggaatt agcttatatc gtgttggaag aatgttttga ggaactggtg
    93061 gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg atctacatgc tgttcagcag
    93121 atattcgact atttacaaac gttgagtcca agccctggcc ggattttcga ccgtagcagc
    93181 gaattgtaca ttattcctga tgtccgggta ttagtggacg acgataaaaa tgtacaagtt
    93241 atctcaaacc gaaaaaatca gccaaatata cgctttcaag agagttactt caaacaaatg
    93301 tcacaacaag cagataaaga aactgaaaat tatttaaaag aacggaaaca ggaattcgaa
    93361 tggttgaaga aaacgatcct acaacgtggg gatacaattt tgcgggttgc tcaagtcatt
    93421 gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc tcttacgtta
    93481 aaggaagttg caactgagat agatgtgcat gaatccacgg tcagtcgtgc agtcaacggg
    93541 aaatatttgg aaacagactt cggtgtattt gaattaaaaa aatttttcac tacacgaatt
    93601 cctacgaata gtacagaaca gacagaagac ctttcagcag atacagcgaa gaaaaagctg
    93661 caggaattag tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa attggttgaa
    93721 ttgctgaaaa aagatgagat agcgatctca agacgaacag tagcaaaata tcgtgattta
    93781 ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata actaatcagt caaaagacta
    93841 gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa acgagagcaa aactgttaga
    93901 cttacagtcg tgaggttgag atttcccttt attgatgtga aattcgaata gttaatttct
    93961 ttttttatcg tatatgggtc gttttaagtc tacattggac gtaaagtgtc caactaagga
    94021 gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt gcaccagata ttatcgaagt
    94081 gttgcaagaa cgctataaaa ttttacgaaa tatctattgg atgcagccga ttgggcgtcg
    94141 aagtttatcg gaaagtatgg ggatcacaga acgagtctta cgaacagaaa cagacctttt
    94201 gaaaaatctg aatttgatcg acacctcaaa aagcggtatg acgctgacaa ctaagggcga
    94261 agaagtttat caaagccttg aaaacttcat ggatcagctt ttaggtacgc atcaaaccga
    94321 acaacagttg gcagaatatt ttggtaacca acgctgtatc gttgtttcag gaaacagcga
    94381 agagcagacc aaagtggctg atgcattcgg cgaagcgctg agcgaagcct tagaccgact
    94441 acttccagaa ggcgagaata tcatcgctgt gatgggagga actactatgg ctgtggtagc
    94501 cgaacaattg tctaatctgg aaaataagaa acggcataac ctgtttgtcc ctgcaagagg
    94561 cgggattggt gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa tggctgctaa
    94621 agcaaacggt aaccaccgcg cactttatgt tccggaacaa ttaagtctgg caacttacaa
    94681 ttctttactc aatgagccat ctatccaaga agtcttaaac ttgattagtg aagcgaattg
    94741 tgttattcat agtattgggc gtgcattaca catggcagca cgtcgtaaga tgactgaaaa
    94801 agaattagtc atgctgaaac aagcaaatgc tgttgcggaa tcttttggct actttttcaa
    94861 tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc atagaattgc aagatttgga
    94921 aaaagtcccg atcatcatgg caatcgcggg cggaaaatca aaagctaaag caattcgagc
    94981 ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact gatgaagctg ctgcaaatga
    95041 gattttaaaa ggggtaaccc tttaaaataa atattttttt gattttcata aggaggaaat
    95101 cttaaatgac agttaaagta ggtattaatg gttttggacg tattggacgt ctagcattcc
    95161 gtcgtatcca agatgtagat ggaatcgaag tagtagcaat caacgacttg acagatgcta
    95221 aaatgttggc acacttgtta aaatacgaca caactcaagg acgtttcaac ggaacagttg
    95281 aagttcatga aggttcattc aacgtaaacg gaaaagaagt taaagtatta gctaaccgca
    95341 acccagaaga attaccatgg ggcgaactag gcgtagatat cgttctagaa tgtactggtt
    95401 tcttcacttc taaatcagca gctgaaaaac atttaacagc tggtgctaaa cgcgttgtta
    95461 tctctgctcc tggcggaaac gatgttccaa caatcgttta taacacaaac cacgaaacat
    95521 taactggtaa agaaacagtt atctcaggtg cttcatgtac tactaactgt ttagctccta
    95581 tggctaaaac attgaacgac aaatttggtg ttgttgaagg attaatgaca actatccacg
    95641 cttacacagg tgaccaaatg actctagacg gacctcatcc taaaggtgac ttccgccgtg
    95701 cacgcgctgc tgctgcaaac atcgttccta actcaacagg tgctgctaaa gctatcggtt
    95761 tagtaattcc cgaattgaac ggtaaattag acggagctgc tcaacgtgtt cctgtaccaa
    95821 caggttcatt aacagaatta gtaacagttc ttgaaaaaga agtaactgtt gacgaaatca
    95881 atgcagcaat gaaagaagct tcaaacgaat cttacggata caacacagac gaaatcgttt
    95941 cttcagatat cgttggtatg acttacggtt cattatttga tgctacacaa actaaagtaa
    96001 tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac gaaatgtcat
    96061 acactgcaca attagtacgt actttagaat acttcgctaa cttataagat agacaaacgt
    96121 acaagtgtaa attatagaca tgtacattta aaagcgggga agcaatcgcg cttcttccgc
    96181 tttttttgat aataagttta ttcaggagga aataaaatgg ctaagaaaac agtgaaagat
    96241 atcgatttaa aagacaaaaa agttcttgtc cgtgttgact tcaacgttcc tttgaaagac
    96301 ggcgtgatca ctgacgacac tcgtatcaaa gcagcattgc caacaatcaa ctacgttcta
    96361 gaacaaggcg gaaaagcaat ccttttctct caccttggac gtgtaaaaac agaagaagat
    96421 aaagaaggaa aatctttagc tccagttgca aaacgtctag gcgaattatt aggtaaagaa
    96481 gtaacattcg tacctgaaac tcgcggagaa caattagaag aagcaatccg caacatgaat
    96541 gacggcgacg ttgtcgtatt cgaaaataca cgatttgaag atattgacgg taaaaaagaa
    96601 agcaaaaatg atacagaact aggtaaatac tgggcttcat taggcgatgt atttgttaac
    96661 gatgctttcg gtacagctca ccgtgcacat gcttctaacg taggtatcgc ttcaactggt
    96721 atcccaacag ttgctggatt cttgatggaa aaagaaatca agttcatcgg tgaagcagta
    96781 gaagaaccaa aacgaccaat gattgcaatc cttggtggcg caaaagtttc tgataaaatc
    96841 ggcgtaatcg aaaacttgat tcctaaagca gataaaatct tgatcggtgg cggaatgact
    96901 tacacattct acgaagcaaa aggaatcaag atcggtaact ctctagtaga agctgataaa
    96961 gtggaactag caaaagaatt aatcgaaaaa gcaggcgaca aacttgtctt gccaatcgac
    97021 aatgtctgtg caccagaatt ttcaaatgat gttgaaacac aagtaatcga aggagacatt
    97081 cctgatggtt tgatggcttt agacatcggc ccagcatcag taaaattatt tgctgataca
    97141 ttacaaggtg caaaaacagt tgtttggaac ggacctatgg gtgtatttga aatgagtaac
    97201 tttgctaaag gtacaatcgg tgtttgtgaa gcaatcgcta acttagaagg cgcaacaaca
    97261 atcatcggtg gcggagactc agccgcagca gcagaacaat taggatttgc tgacaaattc
    97321 acacacatct caacaggtgg cggcgcaagc ttggaattgc ttgaaggtaa agaattacca
    97381 ggactagcag caatcaacga taaataaacg accaagtttg ctcaattctg acgagtaatc
    97441 gaacaaaagg tgaagggagt tttcatgact ccctagccat ttttcaataa attaaggagt
    97501 gtgttcctca atgcgtaaac ctattatcgc aggtaactgg aaaatgaaca aaactgcatc
    97561 agaagcaaaa gaatttgctg aagcagtaaa aaacaaaatc ccttcaaatg acaaagttga
    97621 ttcagtcatc ggatctcctg cattattctt acaagaatta atgacatcag ctgaaggtac
    97681 agaactaaaa atctcagcac aaaactgcta ctgggaaaac tcaggtgcgt tcactggtga
    97741 aacttcacca gcagctcttg ctgatctagg tgttcattat gtgatcatcg gacattcaga
    97801 acgtcgtgaa tatttccatg aaacagacga agacatcaat aaaaaagcga aagctatctt
    97861 tgcaaatggt atgacaccaa tcctatgctg cggcgaatct ttagaaacct atgaagcagg
    97921 caaaactgct gaatggatcg aaggacaaat tacagctggt ttagctgatc tttcagctga
    97981 acaggtgtca aacatggtta ttgcttatga accaatctgg gcaatcggta ctggtaaatc
    98041 agctgacgca caaatcgctg acgaaatttg tggagttgta cgtcaaacag ttgaaaaact
    98101 ttatggaaaa gaagtatcag aagcagttcg tatccaatac ggcggttctg taaaaccaga
    98161 aaatattgct gaatacatgg caaaagaaaa tgttgacggt gctttagtcg gtggcgcaag
    98221 cttgcaagca gattcattct tgtcattgtt agacgctgta aaataattgt tgaaaatttg
    98281 cgaaaaatgc aagaaatgtt tgcactaatg agcaaaatta actaaaatcg tatatgaggg
    98341 aaatccctta ggaaaaaaca aactcaaagg agagacaaaa catgtcaatt attactgatg
    98401 tttacgctcg cgagatctta gactcacgtg gtaacccaac aatcgaagta gaagtataca
    98461 cagaaagcgg agcttttggc cgcggaatgg ttccttcagg tgcttcaact ggtgaatacg
    98521 aagcagttga attacgtgac ggagacaaag ctcgttatgg cggtaaaggt gtaactaaag
    98581 ctgtcgacaa cgtaaacaac atcattgctg aagcaatcat tggctacgat gttcgtgacc
    98641 aaatggctat cgacaaagct atgatcgctt tagacggaac tcctaacaaa ggtaaattag
    98701 gtgcgaacgc tattttaggt gtttctatcg ctgttgcacg tgcagctgct gactacctag
    98761 aagtaccttt ataccactac ctaggcggat tcaacacaaa agtattgcca acaccaatga
    98821 tgaacatcat caacggcgga tcacatgctg ataactcaat cgacttccaa gaattcatga
    98881 tcatgcctgt tggcgctcca acattcaaag aagctctacg tatgggtgct gaagtattcc
    98941 acgcattagc ttcaatcttg aaagctcgcg gattagcaac ttctgttggt gacgaaggtg
    99001 gatttgctcc aaaccttggt tcaaacgaag aaggttttga agtaatcatc gaagcaatcg
    99061 aaaaagctgg ctatgtacct ggtaaagacg ttgttcttgc tatggatgct gcttcttcag
    99121 aattctacga caaagaaaaa ggtgtttacg tactagctga ctcaggcgaa ggcgaaaaaa
    99181 caactgacga aatgatcaaa ttctacgaag aattagtttc taaatatcca atcatctcaa
    99241 tcgaagatgg attagacgaa aacgactggg acggattcaa gaaattaact gacgtattag
    99301 gcgacaaagt tcaattagtt ggtgacgact tgttcgtaac aaacactcaa aaattatctg
    99361 aaggtatcga aaaaggaatt gctaactcaa tccttatcaa agtgaaccaa atcggtacat
    99421 taacagaaac tttcgaagct atcgaaatgg ctaaagaagc tggctacaca gcagttgtat
    99481 ctcaccgttc tggtgaaaca gaagattcaa caatctctga tatcgctgtt gcaacaaacg
    99541 ctggccaaat caaaactggt tctctatcac gtactgaccg tattgctaaa tacaaccaat
    99601 tactacgtat cgaagaccaa cttggtgaag ttgcagaata caaaggtttg aaatctttct
    99661 acaacttaaa aaacaaataa ttcttttcta atgaagatac atgatcgact attgaaagat
    99721 agtcgagtat gatcacttag ttaagtaatc gaactctcta cttgctgaaa tatcagcagg
    99781 tagagagttt ttttgcaact agcttctgta tatgatgttt acaaagaatg gtacaagaaa
    99841 agatcatcct tgaaaaagga aagctaacaa taactagcat catataagag cttttaagga
    99901 tagaacagta gaataggaat ctatgggcaa gtcctctcgt ataaa
//
LOCUS       DACDCJ010000002        97858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDCJ010000002 DACDCJ010000000
VERSION     DACDCJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 97858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.002"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..2122)
                     /gene="pknB"
                     /locus_tag="IV038_000100"
     CDS             complement(53..2122)
                     /gene="pknB"
                     /locus_tag="IV038_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP8340355.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(2119..2859)
                     /locus_tag="IV038_000101"
     CDS             complement(2119..2859)
                     /locus_tag="IV038_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP8340356.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(2872..4230)
                     /gene="rsmB"
                     /locus_tag="IV038_000102"
     CDS             complement(2872..4230)
                     /gene="rsmB"
                     /locus_tag="IV038_000102"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP8340357.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(4230..5168)
                     /locus_tag="IV038_000103"
     CDS             complement(4230..5168)
                     /locus_tag="IV038_000103"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP8340358.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(5161..5652)
                     /gene="def"
                     /locus_tag="IV038_000104"
     CDS             complement(5161..5652)
                     /gene="def"
                     /locus_tag="IV038_000104"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8340359.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(5667..8075)
                     /gene="priA"
                     /locus_tag="IV038_000105"
     CDS             complement(5667..8075)
                     /gene="priA"
                     /locus_tag="IV038_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP8340360.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(8328..8633)
                     /locus_tag="IV038_000106"
     CDS             complement(8328..8633)
                     /locus_tag="IV038_000106"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8340361.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(8638..9252)
                     /gene="gmk"
                     /locus_tag="IV038_000107"
     CDS             complement(8638..9252)
                     /gene="gmk"
                     /locus_tag="IV038_000107"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP8340362.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(9739..10023)
                     /locus_tag="IV038_000108"
     CDS             complement(9739..10023)
                     /locus_tag="IV038_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340363.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(10041..10931)
                     /locus_tag="IV038_000109"
     CDS             complement(10041..10931)
                     /locus_tag="IV038_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP8340364.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(10951..11169)
                     /locus_tag="IV038_000110"
     CDS             complement(10951..11169)
                     /locus_tag="IV038_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340365.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(11139..12839)
                     /locus_tag="IV038_000111"
     CDS             complement(11139..12839)
                     /locus_tag="IV038_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP8340366.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            13105..13878
                     /locus_tag="IV038_000112"
     CDS             13105..13878
                     /locus_tag="IV038_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340367.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(13959..14528)
                     /gene="thiT"
                     /locus_tag="IV038_000113"
     CDS             complement(13959..14528)
                     /gene="thiT"
                     /locus_tag="IV038_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP8340368.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(14594..14696)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(14702..15673)
                     /locus_tag="IV038_000114"
     CDS             complement(14702..15673)
                     /locus_tag="IV038_000114"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8340369.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(15767..17140)
                     /gene="glmU"
                     /locus_tag="IV038_000115"
     CDS             complement(15767..17140)
                     /gene="glmU"
                     /locus_tag="IV038_000115"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP8340370.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(17643..18461)
                     /gene="purR"
                     /locus_tag="IV038_000116"
     CDS             complement(17643..18461)
                     /gene="purR"
                     /locus_tag="IV038_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP8340371.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(18628..19452)
                     /locus_tag="IV038_000117"
     CDS             complement(18628..19452)
                     /locus_tag="IV038_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8340372.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(19425..20105)
                     /locus_tag="IV038_000118"
     CDS             complement(19425..20105)
                     /locus_tag="IV038_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8340373.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(20128..21066)
                     /locus_tag="IV038_000119"
     CDS             complement(20128..21066)
                     /locus_tag="IV038_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP8340374.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(21311..22162)
                     /locus_tag="IV038_000120"
     CDS             complement(21311..22162)
                     /locus_tag="IV038_000120"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP8340375.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(22316..24889)
                     /gene="mprF"
                     /locus_tag="IV038_000121"
     CDS             complement(22316..24889)
                     /gene="mprF"
                     /locus_tag="IV038_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8340376.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            25030..25836
                     /gene="proC"
                     /locus_tag="IV038_000122"
     CDS             25030..25836
                     /gene="proC"
                     /locus_tag="IV038_000122"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP8340377.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(25915..27471)
                     /gene="rny"
                     /locus_tag="IV038_000123"
     CDS             complement(25915..27471)
                     /gene="rny"
                     /locus_tag="IV038_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP8340378.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(27882..28931)
                     /gene="recA"
                     /locus_tag="IV038_000124"
     CDS             complement(27882..28931)
                     /gene="recA"
                     /locus_tag="IV038_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP8340379.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(29040..30281)
                     /locus_tag="IV038_000125"
     CDS             complement(29040..30281)
                     /locus_tag="IV038_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP8340380.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(30521..31099)
                     /gene="pgsA"
                     /locus_tag="IV038_000126"
     CDS             complement(30521..31099)
                     /gene="pgsA"
                     /locus_tag="IV038_000126"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP8340381.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(31308..32192)
                     /locus_tag="IV038_000127"
     CDS             complement(31308..32192)
                     /locus_tag="IV038_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8340382.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(32970..34256)
                     /locus_tag="IV038_000128"
     CDS             complement(32970..34256)
                     /locus_tag="IV038_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8340383.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(34249..35517)
                     /locus_tag="IV038_000129"
     CDS             complement(34249..35517)
                     /locus_tag="IV038_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP8340384.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            35769..36230
                     /gene="mscL"
                     /locus_tag="IV038_000130"
     CDS             35769..36230
                     /gene="mscL"
                     /locus_tag="IV038_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP8340385.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(36289..36735)
                     /locus_tag="IV038_000131"
     CDS             complement(36289..36735)
                     /locus_tag="IV038_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340386.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(36828..37073)
                     /locus_tag="IV038_000132"
     CDS             complement(36828..37073)
                     /locus_tag="IV038_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340387.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(37188..37766)
                     /locus_tag="IV038_000133"
     CDS             complement(37188..37766)
                     /locus_tag="IV038_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8340388.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(37843..38373)
                     /locus_tag="IV038_000134"
     CDS             complement(37843..38373)
                     /locus_tag="IV038_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP8340389.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(38377..39237)
                     /gene="rlmB"
                     /locus_tag="IV038_000135"
     CDS             complement(38377..39237)
                     /gene="rlmB"
                     /locus_tag="IV038_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP8340390.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(39230..39625)
                     /locus_tag="IV038_000136"
     CDS             complement(39230..39625)
                     /locus_tag="IV038_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8340391.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(39622..41034)
                     /locus_tag="IV038_000137"
     CDS             complement(39622..41034)
                     /locus_tag="IV038_000137"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP8340392.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(41031..41567)
                     /gene="cysE"
                     /locus_tag="IV038_000138"
     CDS             complement(41031..41567)
                     /gene="cysE"
                     /locus_tag="IV038_000138"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP8340393.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(41889..43349)
                     /locus_tag="IV038_000139"
     CDS             complement(41889..43349)
                     /locus_tag="IV038_000139"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP8340394.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(43519..44676)
                     /locus_tag="IV038_000140"
     CDS             complement(43519..44676)
                     /locus_tag="IV038_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP8340395.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(44710..46080)
                     /gene="radA"
                     /locus_tag="IV038_000141"
     CDS             complement(44710..46080)
                     /gene="radA"
                     /locus_tag="IV038_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP8340396.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(46143..46670)
                     /locus_tag="IV038_000142"
     CDS             complement(46143..46670)
                     /locus_tag="IV038_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP8340397.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            46900..47706
                     /gene="proB"
                     /locus_tag="IV038_000143"
     CDS             46900..47706
                     /gene="proB"
                     /locus_tag="IV038_000143"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP8340398.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            47703..48950
                     /locus_tag="IV038_000144"
     CDS             47703..48950
                     /locus_tag="IV038_000144"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP8340399.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            49026..49370
                     /locus_tag="IV038_000145"
     CDS             49026..49370
                     /locus_tag="IV038_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340400.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(49487..49813)
                     /locus_tag="IV038_000146"
     CDS             complement(49487..49813)
                     /locus_tag="IV038_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340401.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(50186..51387)
                     /locus_tag="IV038_000147"
                     /pseudo
     CDS             complement(50186..51387)
                     /locus_tag="IV038_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            51504..52019
                     /locus_tag="IV038_000148"
     CDS             51504..52019
                     /locus_tag="IV038_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8340402.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(52218..52664)
                     /locus_tag="IV038_000149"
     CDS             complement(52218..52664)
                     /locus_tag="IV038_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8340403.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(52711..54189)
                     /gene="msr"
                     /locus_tag="IV038_000150"
     CDS             complement(52711..54189)
                     /gene="msr"
                     /locus_tag="IV038_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8340404.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(54543..55172)
                     /locus_tag="IV038_000151"
     CDS             complement(54543..55172)
                     /locus_tag="IV038_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8340405.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(55156..55620)
                     /locus_tag="IV038_000152"
     CDS             complement(55156..55620)
                     /locus_tag="IV038_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8340406.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(56107..57375)
                     /locus_tag="IV038_000153"
     CDS             complement(56107..57375)
                     /locus_tag="IV038_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP8340407.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(57606..58343)
                     /locus_tag="IV038_000154"
     CDS             complement(57606..58343)
                     /locus_tag="IV038_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8340408.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(58410..58979)
                     /locus_tag="IV038_000155"
     CDS             complement(58410..58979)
                     /locus_tag="IV038_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP8340409.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            59107..59979
                     /locus_tag="IV038_000156"
     CDS             59107..59979
                     /locus_tag="IV038_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8340410.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            60321..61193
                     /locus_tag="IV038_000157"
     CDS             60321..61193
                     /locus_tag="IV038_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8340411.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            61190..63148
                     /locus_tag="IV038_000158"
     CDS             61190..63148
                     /locus_tag="IV038_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8340412.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(63399..64250)
                     /locus_tag="IV038_000159"
     CDS             complement(63399..64250)
                     /locus_tag="IV038_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8340413.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            64373..64840
                     /locus_tag="IV038_000160"
     CDS             64373..64840
                     /locus_tag="IV038_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP8340414.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(65103..65585)
                     /locus_tag="IV038_000161"
     CDS             complement(65103..65585)
                     /locus_tag="IV038_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8340415.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(65685..66548)
                     /locus_tag="IV038_000162"
     CDS             complement(65685..66548)
                     /locus_tag="IV038_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8340416.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            67146..67994
                     /locus_tag="IV038_000163"
     CDS             67146..67994
                     /locus_tag="IV038_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340417.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(68165..68506)
                     /locus_tag="IV038_000164"
     CDS             complement(68165..68506)
                     /locus_tag="IV038_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP8340418.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            68592..69299
                     /locus_tag="IV038_000165"
     CDS             68592..69299
                     /locus_tag="IV038_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP8340419.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            69286..69609
                     /locus_tag="IV038_000166"
     CDS             69286..69609
                     /locus_tag="IV038_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8340420.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(69698..70735)
                     /locus_tag="IV038_000167"
     CDS             complement(69698..70735)
                     /locus_tag="IV038_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8340421.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(71182..72165)
                     /locus_tag="IV038_000168"
     CDS             complement(71182..72165)
                     /locus_tag="IV038_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP8340422.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(72581..73351)
                     /locus_tag="IV038_000169"
     CDS             complement(72581..73351)
                     /locus_tag="IV038_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340423.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(73344..74279)
                     /locus_tag="IV038_000170"
     CDS             complement(73344..74279)
                     /locus_tag="IV038_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340424.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(74873..76522)
                     /locus_tag="IV038_000171"
     CDS             complement(74873..76522)
                     /locus_tag="IV038_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8340425.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TILAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(76717..77613)
                     /locus_tag="IV038_000172"
     CDS             complement(76717..77613)
                     /locus_tag="IV038_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8340426.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(77606..78286)
                     /locus_tag="IV038_000173"
     CDS             complement(77606..78286)
                     /locus_tag="IV038_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8340427.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(78287..79069)
                     /locus_tag="IV038_000174"
     CDS             complement(78287..79069)
                     /locus_tag="IV038_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8340428.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(79062..79979)
                     /locus_tag="IV038_000175"
     CDS             complement(79062..79979)
                     /locus_tag="IV038_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8340429.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(79982..80266)
                     /locus_tag="IV038_000176"
     CDS             complement(79982..80266)
                     /locus_tag="IV038_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8340430.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            81054..84281
                     /gene="fss3"
                     /locus_tag="IV038_000177"
     CDS             81054..84281
                     /gene="fss3"
                     /locus_tag="IV038_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP8340431.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            84393..84707
                     /locus_tag="IV038_000178"
     CDS             84393..84707
                     /locus_tag="IV038_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8340432.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            84720..85094
                     /locus_tag="IV038_000179"
     CDS             84720..85094
                     /locus_tag="IV038_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP8340433.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            85104..85448
                     /locus_tag="IV038_000180"
     CDS             85104..85448
                     /locus_tag="IV038_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP8340434.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            85517..86533
                     /locus_tag="IV038_000181"
     CDS             85517..86533
                     /locus_tag="IV038_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP8340435.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLSALFIGWMGVFYFPELRQFGL
                     SKVSFSTIEIAWSMKDLVCLLGSLALSGSMMLLYIHFFPDHWRSLWNRQKLARMILEN
                     HWYEVKQTQSDGFFKDLNRSRTKETISYFPKVYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISKCVEDFYERMMARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            87127..89001
                     /gene="ltrA"
                     /locus_tag="IV038_000182"
     CDS             87127..89001
                     /gene="ltrA"
                     /locus_tag="IV038_000182"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8340436.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            89127..89483
                     /locus_tag="IV038_000183"
     CDS             89127..89483
                     /locus_tag="IV038_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8340437.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            89528..90670
                     /gene="dcm"
                     /locus_tag="IV038_000184"
     CDS             89528..90670
                     /gene="dcm"
                     /locus_tag="IV038_000184"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP8340438.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            90660..90949
                     /locus_tag="IV038_000185"
                     /pseudo
     CDS             90660..90949
                     /locus_tag="IV038_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            91205..92389
                     /locus_tag="IV038_000186"
     CDS             91205..92389
                     /locus_tag="IV038_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8340439.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            92404..92499
                     /locus_tag="IV038_000187"
     CDS             92404..92499
                     /locus_tag="IV038_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP8340440.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            92528..92749
                     /locus_tag="IV038_000188"
     CDS             92528..92749
                     /locus_tag="IV038_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340441.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            92762..93265
                     /locus_tag="IV038_000189"
     CDS             92762..93265
                     /locus_tag="IV038_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP8340442.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            93392..94429
                     /locus_tag="IV038_000190"
     CDS             93392..94429
                     /locus_tag="IV038_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340443.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            94444..96141
                     /locus_tag="IV038_000191"
     CDS             94444..96141
                     /locus_tag="IV038_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP8340444.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            96134..96595
                     /locus_tag="IV038_000192"
     CDS             96134..96595
                     /locus_tag="IV038_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340445.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            96608..97468
                     /locus_tag="IV038_000193"
     CDS             96608..97468
                     /locus_tag="IV038_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340446.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            97506..>97858
                     /locus_tag="IV038_000194"
     CDS             97506..>97858
                     /locus_tag="IV038_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8340447.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEP"
BASE COUNT        28662 a        20056 c        18053 g        31087 t
ORIGIN      
        1 agaatgcaac aaatcaatgt cgcattccct tctgatatat tttattcagc acttattttg
       61 aactttctga ggtgctagct gatgaacttg tggagctgga agattcttct tttgaagagt
      121 tgcttgtgct actggagcta cttgagctgc tagaactgtc tgaagagtct ttttcagaac
      181 tagaagacgc tggatctggt cctttagagt aaattacgtt cacaaccatt cctttggtaa
      241 ttgtcgttcc tgcccctggt actgtacgaa tcactttatc tttatccacc gtgtttgaat
      301 attcggaagt ctcatgcccg atatattctg caccgacact agctaaataa ctttgtgctt
      361 cgctttttgt atagccggag atatcattca atttaggctc tgttccttgg cttacgacaa
      421 aattgatgta agtttcttct gctacaactt cacccgaggc tggatcctga ctgatgatcg
      481 aacctgatgg tacagtgtcg ctatagtcat aagtagcact aatattattg atattgaatc
      541 cttgtgtaac aagccaatta cgaacagttt cgtagttttc tcctacatga ctactcaaag
      601 tcactgtttc tggtcctttg ctgactgtga aagtaatcgt gtctgtttta ggatcgactt
      661 ctgtatcttc tgatggagat tgcgaaatga tcattccgct ttctacctct gaactgaatt
      721 cctcttcttt ttgaatcaag tcagcagaaa accctaattc tttcaaactt gcttcggcat
      781 cttcataact tttaccactg taatcatcta atttgatttt tttcgtgccg ctactaatat
      841 acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct gtcttcacta
      901 cattaccttc ttcgattttg tcatctgcaa tttcttctgt ttttgttgct acttctaatt
      961 tcgcttttgc gagtgtttct ctagcagaag cttcagaaag atcagaaaca tcaggaacaa
     1021 caacttctcc tcgcccgcca gaagcaaaga atacagctgt tccgattcct aaaacagcaa
     1081 gaatagctaa taggatcagc caccattttt tctgtccttt ctttttagga tcaggcagaa
     1141 cttcttcctc ctctggtaag gtttcgtttt ctggcttgtc ttttggcaga ggcatagatt
     1201 tgaatgaatc aggcattggt gcatcttccg taaggggtgt gataactttt gtttcattat
     1261 ccatcacatg aggctgccat ttttcttcgt ttgcacgagc tggtgataat actgtagaaa
     1321 gatcatcaga catttcctcg gctgacttat aacggtctgc aggttcttta gccgttgccc
     1381 gtaaaatcac gttttccagt gcttgtggca catttggatc aagtgctttt atagatggaa
     1441 gatcatcttg aaaatgtttt aaagcaattg ttacagcaga ttcaccatca aacggtacgc
     1501 tacccgtcaa catctcataa aggatgatcc ctaaagcata aatatctgac tgcttcgttg
     1561 ccatacttcc tctggcctgc tcaggggaca gataatgaac agatcctagc atcgtattcg
     1621 tctgcgtgat cgatgtttca gataaagcga tagcaatccc gaaatccgtg atttttacaa
     1681 caccttcatt atcgatcagt acgttttggg gtttcaaatc tcggtggatg atttgatggt
     1741 tatgagctaa agaaatagca gaaaggatct gctgcataat atttactgct gtttcataag
     1801 gaacaggata atgtgtttgg atatatcgtt tcaagtccat tcctttgacg tattccatca
     1861 ccagatactg catgttgtct tcttcaccaa catcgtaaac actaacaatg tttggatgaa
     1921 cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc tggtcatttt
     1981 gaaagtcaaa tcgtaagact ttaactgcta catcacgatc caagattaaa tcgtgcgcca
     2041 aaaatacatt tgccatacca ccgctaccga tatttccagt gatttgataa cggccattta
     2101 attttttgcc caactcgatc atacactgtc ctccttatat tcgataagaa gaacggtgat
     2161 attatccgct ccgcctgctt cattggcacg atcaatcagc tcagttactt tatgagacaa
     2221 tgggccttct tgattgatga tcgttcctat ttcttcttca gacagcatgt tcgtcaagcc
     2281 atctgaacat aacagcagtc gatcttttag ttgccaaaga tgagtagaaa catctacttc
     2341 gactgttcca ggcatgccga cagaacgtgt taaaatattt ttccttggat ggttcactgc
     2401 catttcttcg ctgatttctc ctgatttgac tagttcattg actaaagaat gatcctccgt
     2461 caattgcatg attttatctt cacgaatcaa ataagctctg ctatccccaa cattggcaat
     2521 cgtgaaccag tcatctgaca gagcggccgc tacgattgtt gttcccatac cgttatattc
     2581 cggctgttct tgtccacgct gataaatcag catattttct tcttgaatcg attgaatgaa
     2641 ccattgggcg attttttcat cgtcaacagc ttcttgtttt tcccaagctt cgcctagatt
     2701 cgttaccgcc atctgactgg caatgtctcc ggcacgatga ccacccatcc cgtctgctaa
     2761 tacagcaagc ttgagacctg aacggctggt aaatacactg gcatagtctt gatttgtgtt
     2821 acgtcgtctt cctacatctg attgatattc aatctgcatt ttttcacctc attacttttt
     2881 gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga tgcggataca aagtcaacat
     2941 tttctcttcg attgatgatt gtagcatagg attggtcaaa acatcaatcc tctcaaaatc
     3001 agggtgtctt tccaagaaag cagaaacaac gtcttggttt tcttctttta agatcgtaca
     3061 agtgctataa gtcagaatac cggaagactt taaagttggt gcgcagcttt ctaaaatttc
     3121 caattggatt tttggtaaat tcgctaattc atccggctgt tttttataac gaatatctgg
     3181 ttttcgccga agcagcccaa gacctgaaca aggcgcgtct actaaaatcc gatcaaactg
     3241 ctctgcttcg aactgtttac tgacttctct agcatccatc ttttgagcgt agacagcaga
     3301 agagacacct agacgtgctg cattatcttc aatgagtttg actttgtgtt cgtgtacatc
     3361 taaagcgacc acttctccac cgcatgacgg atctaaaaaa gaagcaatat gcgtcgtctt
     3421 acctccgggt gctgcacagg catctaagac atgatgttca ggctcgattt gcatagacgg
     3481 cgctacaagc atcgaacttt catcttggat cgtcatgacg ccatcgtgga acaatgagct
     3541 agaagctaaa tgcccttttt tcgctactat accgtatgga ctgacttggc ttttttcagc
     3601 tgtgatctct tcttcttgca aaacagtgat ggcctgatct ctggaaagtc ggcgtgtatc
     3661 tactcgggca ctagcatggc taggctgata tagcgataaa ccgagctttt cagtttcttc
     3721 gtatccgatt tgatccacca gtcgtttagt catccataga ggcaggctga ttttgacaga
     3781 aagtctttcg ataggatcag caatgccttg cagatctggt gctccttgac gctgataatt
     3841 cctcaagaca ccatttacaa atttccctgt cccaggattt ccttttactt ttgcgatttc
     3901 gaccgcctcg tttaaaatag catgtgcagg gactttatct aaaaattcta attggtatac
     3961 agaaagataa agaagcgtct tgacccagtc atccactttt tttgctttag cgatcaatgg
     4021 tgcaacgtaa aattccaata gtcgctgatg gctaatcgta ccatagatca attctgtaaa
     4081 taaacccaca tctttttgat ttagctcgct ttgtttgatc atctcattca acaaaagatt
     4141 cgagtaggcg ccacccttgt tcactcgctc taatgtcagt aatgccatat atcgtactga
     4201 ttttttcatt tttttaggga tttttttcat tatttcacct gctgaccaat ctgaacttgc
     4261 tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc ctttaccagc tggctgtaat
     4321 tcattgatgg ctaagacggt ctgtttaccg caagcaatcc atagattttt tttgtctttc
     4381 cggatgatcg taccaggtgc tttgtctgta gtttctgcca atacttcaac cgataataat
     4441 ttccagcgta tgccctcata ggtcgtgaat gcaattggcc aaggacgcat tccgcgtact
     4501 tggttgtcaa tttcttctgc agttttatgc caatcgattg cttcttgttc ccgtgtgatg
     4561 tttggcgaaa aagttgcttc tgactcgtct tgaggtactg gtttttgtcc atccaaaata
     4621 ttgggcaatg tttctagcaa tagctttctt cctaaaaggc taagcttttc aaacatcgtc
     4681 cctacatcat cttgtttagt gattggtatg ctttcttgcg catagattcc gcctgcatcc
     4741 atttttttga tcatttccat gatcgttaca ccagtttctt tctccccatt catgatggca
     4801 taatgaacag gcgcacctcc ccgatattta ggaagtaaag aagcatggac attgattgcc
     4861 cctaattttg gtacttgcaa taacttttca ggcaagaatt ggccaaatgc cgcagtgacg
     4921 atcacatcag gcaccaattc aatgatttct tccatttctg gcgaaccgga gattttctct
     4981 ggctgcagca cacggatacc gtgcttcaga gccgcttctt ttaccggtgt cggcgtaatt
     5041 actcgtttcc gcccaactgg acggtcgggc tgtgttacca ctgcctggat ctcgtaacct
     5101 gattctatca gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat aaatactagc
     5161 ttagtcatca agatgttcct ccatatattc ttcaagttct tcttccggga tctgctgtat
     5221 cattttctca atgaacaaga taccgtctaa gtgatcgatt tcatgctgga aggcacgggc
     5281 taaatagcca aaagccgtca cttctatctc ttctccgtct cgatcataat aacgcacggt
     5341 cacttcatct gctcgcttga ccgttccata gacatgcgga atactgagac aaccttctac
     5401 gtcaaggctt tcaccttttg cttcgatgat ttctgggtta atcagttcaa atttctcgcc
     5461 ttcatctact tcgatcacag caattctctt gttttgtcct acttgaggag cagcgatccc
     5521 tattccgtca ttcgcaatca ttgtctcata taaattgtct aataaagcga ttgtctcgtc
     5581 cgtgattaca tcgattggct gagcagttct ttttaattta tcattagggt ggatcaatat
     5641 aggatagcgc ataatttggc cctttcttaa ataaagttta atggttcgtt gtcaattgcc
     5701 acaaaaagac cttgtcgctg ttctttctgg cttccattca aaatttcatc caaaacatga
     5761 ggaagattcg gttcatgttt atatttgatg attagttgat aataatatcg gtttttcact
     5821 ctcaaaatca tgctgggcgt tggccctaat agcaagcttt gagggcttaa cacttctttt
     5881 agctgattgg caatctgaaa gattttcttt gccgctactt gttcttcagg atggctggcg
     5941 gttattttca cagtaaagta ataaggagga tagcctccac gatgccgtac atgcatttcc
     6001 tgctgataaa actgctcata attttgtttt ttagccaatt ctatggcgta atgttgagga
     6061 ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc gccctgctcg tcctgaaact
     6121 tgggtaagca attggaatgt atgctcacta gaacgaaaat ccggtaaatt cagggaggta
     6181 tcagcattca acaccccaac taatgtcact tctggaaagt ccaaaccttt agcaatcatt
     6241 tgtgtcccta ataatatatc tgcttctttt gcaccgaatt tttgtaaaat ctgttcgtgt
     6301 gccccttttc ttcgagtggt atctacatcc atacgcaaaa tcctagcttg cggataaagt
     6361 tctcggagtt cctcttctac tttttgagta cctgttccat agtagcggat tttattgccg
     6421 ccacaattag gacagcgatt gggaataggt tcttcatgtc cgcaataatg gcagcgcatt
     6481 gaacgtgtat ccatatgcag tgtaagagag atatcgcaat taggacatgg aagaacaaat
     6541 ccacagtccc tacacatcac aaaagacgaa tatcctcgac gattgagcag taggacagtt
     6601 tgttcttttt tgtctaatcg attttgtatt ttttcctgca aattagcaga aaaagtagag
     6661 gtccgatgat tctgaaactc ttcacgcata tctacgatct caatagctgg aagctgtgca
     6721 gctgctttgg ctcggtggtt taactgcaat aactgataaa cgcctttttg agctcgtgct
     6781 cttgattcta aagagggcgt cgcgcttcct agaactattg ggcaatgatg atatttactg
     6841 cgccaaattg ctagatctct cgcatgataa cgcggtgtct catcttgttt gtaacttgat
     6901 tcatgttctt catcgataat gatcagcccg atatttttca gcggggcaaa aatagcagat
     6961 cgtgcgccta ctactacttg cgcttcttcc cgctcaatct tgcgccattc atcatatttt
     7021 tcaccttggg aaagtccgct atgtaagacc gcaactgatt ctccgaagcg tcctttgaat
     7081 cgctcgacca tttgcggagt caatgcgatt tcaggcacta acataatagc cgttttattt
     7141 tctgacaaaa catcagcgat tgcctgaaga tatacttctg ttttcccgct gccagtgatt
     7201 ccctccagca aaaagacctg atctttttgc tgctgacctg ctgtgatgat ttgttcgaca
     7261 gcattttttt gttctgcatt cagttccaat gccgtcgttt ggtcaaatac tcggtcttta
     7321 taagggtcgc gataacgttc tgattctata aactccagcc agcccttttc ttttccttga
     7381 ttcaagacag cagtactgat tcctttattt ttgaaataag caattggttc ttcttggctc
     7441 agttcagata aacacaaaag aagttctttt tgtttcttgg cattttgccg taattttgcc
     7501 cattcttctt cgatttgctt ttcagttaac aaagaacgga ccaaacgaat cgtttttact
     7561 ttatttcgtg tatggacttc atatttgata tctactaatt gctcttggcg ccagcgaatc
     7621 aattgagaaa ggctgccttc ttcttctgct tccttccagg aaatcacttc tcgctgatca
     7681 aacaatacct gaacttctgg tgtgtctgcc aaaggataga tcaatttatc atattctgct
     7741 ttcatcacac tcggaagcat cgtttgtaga caagtgattt tgaatgcgta agtaattttt
     7801 ttcatataat cagctaatga aagtaattct tcattgacca ccggagcaag gtctagcaaa
     7861 cgaataactg ccttcaaaga tggattagct gattcttctg atttttggat agccataaca
     7921 aatccttgga catgacgatt gccattacca aaagggactt ctactcgcat cccgacttgc
     7981 aaagctgttt ccatttcaga gggaaccaaa taagtgaatg gctgatcggt ctgcatagta
     8041 ggtacgtcca caatgacttc agctgttaat gtcatggttc gtttcctcct ttttttgtct
     8101 tttcttatca agcgacaaaa agttccttgc ctattttact agagatcgat ggatttgtct
     8161 tatcttctgc caaatcctaa caaaaaatta acagcagata agaaaaaaaa gaactgtgtc
     8221 aaaaagttca gacacagcct tttctaccag acaataagtg gcgaatgttt gaaaaggaaa
     8281 cagctgggtt tgcttttctt tttagatgtt cgctctattt ttctttatta cattttttgc
     8341 tcttcacgga tacgtgattc caattcgtgt tgttcacgct ctttttgtgc ttgacgttcc
     8401 tcttcttcca tcttcaaacg ttcacgtttt aattcaggat gtggatcatt gactacattt
     8461 cctgcatcga tttcttctaa agcttgaccg acgcttttta ctgaatcaaa agaatctaga
     8521 gtaggctgtg cacttgcatc caattcatgc gcgcgtttac ttgctaaaat cactagagaa
     8581 tattttgaat ttacacgatc taatagtgag tcaattgaag gttttaacat cataagacta
     8641 catctccttt aacattttaa tatatttacc gattacgcga tctactcgaa aatgttcact
     8701 ggcaataatg tttttgattc gttcaactgc tagcggcact tcatcattta caacagcata
     8761 gtcgtaaaga gccatcattt cgatttcttc ttttgcaact ttcattcgtt cttcgattac
     8821 ttcatctgca tctgttcctc ggcctacgat ccgtgatttt aattccgcta aatctggcgg
     8881 agtcaaaaag atgaatacac catcgggcac tttttctttg acttgttttg ctccttggac
     8941 ttcaatctcc aagaaaacat cttttccttc atccaatgtc ttgttgacat atgacaaagg
     9001 agttccataa taattcccga catattctgc gtattccagc atctctccag cttcgatcat
     9061 tgattcaaac tcttctttgc ttcggaagaa ataatcaacg ccttcgacct ccccttcacg
     9121 cattttacgg gtagtcattg agatcgaata ctgaaaatca ttatcctcac tctcaaaaat
     9181 tgcttttcgt actgtgcctt ttcctacacc ggatggacca gacaacacga ttaataatcc
     9241 acgctctgac atgacgacgt cctttcaact agtctttttg tacttatcgt ttattttgca
     9301 ctttttttct gcaactttca aggttatctt taattatagc tagaaattgg taaaaaagct
     9361 accctgtacc aagaggtcgt aaaaaagctg tcctgcatca agtaataaga acagccaaac
     9421 ataaacagct tctcatgttt ttcgtttggt tggggttatt atcgcagatg caagcttttg
     9481 aacgccgtgt ataagaggtc gtgaagaagc cgattagttt cgagtatgaa gaaaaatttt
     9541 gtgaaaatag tttctcacat ttttgcataa atttggctta ataccgagaa aatggttttt
     9601 gaacatcact tattcaaaat aaaaaaagat tgtgacacga attttgtcac aacctttaaa
     9661 tgtattccta gagcaaagaa gctaagcgaa gaaatccaat tctgattatt tattgtattt
     9721 ctttgttcag catccttgct aaaagcccat attggacaaa gtattcacta cacccatgat
     9781 ctcatctttt cgagaacgtg ctttttctgt cggatcttct acctcttctg tgtcaattcg
     9841 aacaataaat ttccgattca tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc
     9901 gttctcatta tagtctgcac aggcatcttt ccatacgtca aagccgtttt tttcagaagg
     9961 gatatcccgt ttaaccacag tttttccact taattcgtgg aacgttacgt cataatattt
    10021 cattatctct acctctttcg tcattcaata ttttgtactt gttctctgat tttttctaag
    10081 atcgtcttag actggacgac ttgattttta atttcgattg catttgattt ggaaccgatc
    10141 gtgttgattt cccgattcat ttcttggatc aggaaatcta attcccgacc tacaggacat
    10201 tgtgcttcga taagttcatc tagtttttgt acatggatcg ccagacgatc cagttcctcg
    10261 tgaatatctc cgcgttccaa caagatggct agctccgtca acaaccgttg ctcatcgact
    10321 ttcgctccaa gaaattcctc cagctttttt tgatacctta tttgaaattc cttttcatag
    10381 atttctacaa aactatcgag tttttttagt acttctttga attcttcagt attctttttc
    10441 aacactaaag ctaaagcttg accttctttt tctcgactcg cctcaataga atcaagtgcg
    10501 cttttcaatg tatctagagc taactgtgcc aattcttcca tgctctcttc cggtttttct
    10561 tctaccgtta cgaagttttc gtccagcgcg aagcgttccg ccatccgact aattgacact
    10621 tgattttgac taccgtatcg ttgttgaatt ccgtctgtca attgagtgat aagtgaatca
    10681 atcaattccc agttgatgac gatctgtttt tcgtttttgc caatataagc tagattcaca
    10741 aatacttcga ttcgaccgcg gcttaactgc tgtttaataa gctgacgaaa atcgttttca
    10801 acaaagttca ggattttagg acttcgtatt tgaacatcta gaaaacgctg gttgacactt
    10861 tttatttcaa tatctaactg atacgctttt gtttcgcgga tggctttgcc aaaaccagtc
    10921 atacttttca ttcgacaggg tccttcctca ttacatattt tctattgctt tttgcagttg
    10981 ttcaaactct tcgttcgtca atgtcaggcc tttccccatt ttttcatggt ctggcgccca
    11041 atctcgaaga tcgaattttg gcgggcgacc attccagctg actaggttca gttctttacg
    11101 ccaccccttg ttgttttctg acaatatagc gatttcttct acgatttcat atgaaaattc
    11161 ttgtgccatg gttgttcctc cttgagataa tataaatata ggatcagtaa tgtgtcaact
    11221 ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt ttccttgtag cagataatca
    11281 aaataaacag ttttcgtgcg ctcattttgt attttttgct ggtattttcg ataccttcgc
    11341 cattcttcag tacttaattg accaatttgt gccaataaat catccggcat actttttata
    11401 tcttgttggc agatcatttg aatcatcatg tctacttgat agatttcatt gggcagtaaa
    11461 aaatgttttt ttgctaaaag ttctagcatc gccaatccgc atgtctctgg tacgaccctg
    11521 ttcttatata aattacgcag tttttgccaa aattttatgc ttttgaaaaa agataattct
    11581 aactgaattt cttttgttaa gtaagacaaa aatcctcgcc agtatgttct tgcaggttta
    11641 acagaatgaa atgtatacca gttcccatag taatagccta atcgtttttt catttccaga
    11701 tagcctaatt gtaaatttaa ttggttcctt tggctatcaa aaagcagaaa gctgcctagt
    11761 gtccacaatg tttgacattt ccaatggata aaaatgtctg gcatacgaat ttttttcgct
    11821 gggcctggcc cttgcacatc tacgacaaat gcctctgttg ctcctttgtt aatcgcaata
    11881 tcaataggta ttttgttgcg atacccaccg tctgcatatt tattttttcc gatctttcga
    11941 taagccatag cgggataaaa tgaagcagag gcgagtatcc aatctggtat ttcttctgtt
    12001 tttgtctgtt ggatatcagt cactacttcc gctagttctg gcagtttagt agacaccgta
    12061 tataaacgta ttttgggatt atgtttgagt aaggacaaat ccaaattgtc tttgaccatc
    12121 ttttccaatg gagcagaaga aaccccgcct tcaattagtg ctgttttcgt catttggcgt
    12181 ctttctcgat agaagcgttc acgaaggtct tcaactgcag ccatttctgg taacgcaagc
    12241 acttgtcttg tcgacagctt tttccataaa ttcacagctt ttttcatatc tccttgtaaa
    12301 atcaatgcgc cgttcaacgc acctaccgag gtacctgtga taatctcaaa gttgatatca
    12361 tgttccttca gcgcctgcca cacaccaatc tgataagcac cgtgtgctcc tcctccacct
    12421 aaaacaagtg ctgtatgata gaccagcttt ttatttaatt gtgtggttcc ttgatagccg
    12481 tttttatgaa aaatacgcaa ccattcttct gatagaggag attgaaataa aaaacaacag
    12541 ttttctttaa taaaaaaacg agcgcaagct tccatatacc gaaaaaattt tttccattca
    12601 aaagtttcac cttcaatcaa cagattatta aaaataacat ctcctctact tttagcaata
    12661 gtaaaataga cggtatattt tttcgatact gctttaaaag cacattgctt ttttgcagaa
    12721 cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa atggaagggg ttgttttgct
    12781 tgctggatag gcgaatacag cgacattttc tgtgaaaagt aaactttttt tatctccatc
    12841 gcgctctcct ttttcttcaa gtataccagt acgtcataaa aaggtcatga gtctcgtatt
    12901 cgactttgta gattccataa gaaaacaatt cataaaacat cctttaaata aaaaatacaa
    12961 aaatcacaaa aattaattga aaatgagagt gttttcaatg aaaacgtgat tttgtaatgg
    13021 aattttaaaa taaaagaaac aagctttttt aatgcaaatg ttgtaaaata aaagagatta
    13081 agaggaaata agaggaggaa catgatgact gagtcagttt atgtacatat tgataccaca
    13141 agtaatgctg ttttaacaaa agggctgact gctactgact ttgcaaacag tatcgtgcat
    13201 tttcctcaaa acttgctctt gcttgatcca tctgcttctg ctggagaata tgaatcccat
    13261 acagggctga aagtaatccg tggtacagaa aatattcagc gtttcttttc ctcttctcgt
    13321 aacaggcgtg gattcgatga attgaagtgg atcgacttta ctgatttaac gatgctgaaa
    13381 gaattgacac ctttagaaat ttcagaatta ttatattttg gtcatatgaa aacacatctt
    13441 cattcgccgt ttttttataa actacagaat aattttgtat acttcgatct taatgatcag
    13501 ctgaatcgga tttattatcg ctatttagat gaattttata ggatttttgc tagtaaatta
    13561 agttcgatct tgtcagataa gatcaatgta aaaaaatctt ttttcaaaaa agaaacacct
    13621 gttgaaaaaa tcagtgtgga attattgaaa gaaatgcgta atttgatgca ggaagggatc
    13681 attttttctt ttgatcaagt tggtttgatc gatggtaaat atcgtatccc aatctatgtg
    13741 attgaagatt atatttggaa attaaaaggc acccggtatc gagaagagga aatcatcgct
    13801 actttaatct actctgtcac cgatcgacac tggcaattgg tagaagatgc cgatctttct
    13861 tatctgccat tgaagtagat ctatttctta tcaaaaaata aaaaggaaac aagaaactag
    13921 gacatgattc tgtctcaagt ttcttgcttc ttttttattt acagctcctg tcgtttatct
    13981 ggcacaaaaa tcgaaggagt cgtttgttgg atccctaata aaacgatgat cgttacaata
    14041 gctgtactca acccgctggc accattcatt acgaaagaaa agagccaaga attcatcccc
    14101 catagtgcat acgctcccca gaaaaccact cctgcaataa aatgccaaaa atatctggaa
    14161 agtgcgccaa tcaatgcacc aaacacgatg ctttttctaa tttgtttcgt attatcgtct
    14221 acatagcctt tttggacatt tgaagccatg agccctgctg cgcctgcaaa agtataggca
    14281 atcgggtact caatcaacac ttgaacgaca cttaaataat aaacttgtcc caacggaaaa
    14341 tgtaggatgc cccaaatgaa acctgccagc atccctgcct ttgtccctct tcttaacgca
    14401 aaaagagtca atggaatctg ccctaatgaa atagaaaaac tgctgccgat ctggatcgga
    14461 atcaacgaca acaccatcgc taacgctgca acaatcgttc cttctaccca aactctattt
    14521 tttcccataa aaaaacttcc tcctttgaat aaaaaagtca caaaggagga agcagctttc
    14581 gaaaatagtc aaacagctgt ttattcacaa ttcctacgct cgcataaccg atacaggtac
    14641 gaagggttta gaaatagatt caatctcagc ccaattggac tcccctttgt gagtttattt
    14701 attatttatt ttttgtttca aacaatggac taactggttt gttttcatgg atacgtttga
    14761 ttgcatctcc cattagtccg ccaacactta cttcatcgat tttgtcgatt ttacgatctt
    14821 ctggaagata gatcgagtct gtaacaacta gacgctcgat agctgaatct tctattcttt
    14881 gtaaagcagg gccggataat actggatgtg tacatgaagc atacacacta gttgcaccag
    14941 cttctttcaa agcattggca gccaatgtga tcgtacctgc tgtatcgatc atatcatcga
    15001 tcagtacaca agttttgcct ttgacttgtc caatgatatt cattacttct gcaacatttg
    15061 cttttggacg gcgtttgtct atgatcgcaa tcggagattt caaaaattcc gctaatttac
    15121 gtgcccgtgt gacaccacca tggtctggtg aaacaacgac aacatcatcg cctttgattc
    15181 ctttttctaa gaaataattg gcaataagtg gcgcacccat caaatgatcg actggaatat
    15241 caaagaaacc ttggatttgg actgcatgaa ggtccaaagt cagcattctt gttgcaccag
    15301 ctttttgaat catgtttgct actaatttcg ctgtgattgg ttcccgtgca cgtgctttac
    15361 ggtcttgacg cgcatagcca taataaggca tgactacgtt gattgttttt gcacttgctc
    15421 gtttcagtgc gtcaatcatg atcagcaact ccatcaagtt atcattgact gggctgctcg
    15481 ttgattgaat aacatagaca tgcgatccac gaatactttc ttcaatgttt acttgaattt
    15541 ctccatcact aaattgagta acagatgatt ttcctaactc gacgccgact gctgctgcaa
    15601 ttttctctgc taatgggcga ttggaattta acgcaaaaat ttttaatctt gggtcaaagt
    15661 aatgttttga catggggacc tccactttct ttttcacaac tctagaattc tacctaataa
    15721 atagtagtca tattttttaa ataaatcaag tgattttgca aaagtcttag tttagataag
    15781 ggagtttttt tgcatatcct tctttattga cttgtcttgc tctcgcaatt gccagcgcat
    15841 attctggaat attgtctgta attgttgacc cggcagcaat cgaactgttt ttagctacct
    15901 cgacaggtcc gatgatattc gtgctagaac caataaagct gtggtcgccg acagtcgtat
    15961 gatgtttgtt ctttccatca tagttgacga agacaacacc acatccgaca ttgatttctt
    16021 ctcccagcgt agcatcacca acataagtca agtgaccgac tttagttcct ttgccgattt
    16081 gagcattttt cacttccaca aagtttccga tatggacgtt ttcgccaatt tcagcttttg
    16141 gtcgtaaatg tgcataaggt cccacatctg catgtttctt gacatggctg ctttcaatga
    16201 ctgagttttc aatgacgacg tgatcctcaa tcctactgtc tacgattttt gaatgtgacc
    16261 cgatgacaca atcagaacca atgactgtat ttccttggat ttgtacacct gcttcgatca
    16321 atgtgtctgg accgatttct acgcctgcat cgatgtatgt cgttgctgaa tcgacaaagc
    16381 ttacgccatt gaccatatgc atctggttaa ttcgctttct catgatctca tttgctttag
    16441 ctaacgcgat ccgatcattg actcccattg attcttcaaa atcatccgtt tgataagcag
    16501 caactgtatg gccttcttct tttaagattt cgatgatatc tgttagatag tattcacctt
    16561 gagcattgtt tgttccaact ttgcttaacg catcgaacaa agcttcgttg tcaaaacagt
    16621 aagttcctgt attgatttct tgtacaagag cttcttctgg tgttgcatct ttttgttcca
    16681 cgattttttc tacgatccca atatggtcac ggatgattcg tccatatcct gttgggtttt
    16741 ctgcttgtgc cgtcaagatc gttgcacttg cattttttcc ttggtgatat tcaaaaagat
    16801 tgttcagcgt ttcagtagtc aataatggtg tatcgccact gataactaac gttgttcctt
    16861 tttttccttc caagaaagaa gcagcttgca aaacagcatg tcctgttccc aattgttctg
    16921 cttgtaacgc atatttactg cgttctccta gttgtgcttt aacttgttct gctccgtgtc
    16981 caacgatcgt aatcacttca tctggtttcg tttcactgac ccgattaatg atatgttcta
    17041 ccataggttt accagaaacg ggatgtaaaa ctttatataa ttttgacttc atgcgggtac
    17101 ctttccctgc cgccaaaata attgcataac gtgcgtccaa ctgcgtcact cctaaatata
    17161 gtttgctgat ttcagtttat cccagtatac ccattttttc aacaaaacaa gtgattttct
    17221 tttatcccag cgttttctgg ataaaaaaat agatagacca taacgggcct atctatttgg
    17281 taaccggtgc tctcctgcca actcctcggt cacaagtcac actactcata aaacatgaaa
    17341 agctgttttt tgaaatcgtt tcttgtgact tagagttaaa cggtaggctt acaacctctt
    17401 cattaaacgt ttttcaatca gctgaccttc ggtattagct caataaacgg caaaatatga
    17461 ggagcaattt ttccgtttat tgagctaata ctcaggacag gacgctgatt tcacaacctc
    17521 tagataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gaaaaaatgt
    17581 ggaaagccat tttcacaaaa tttttcttac tactcgaaac taagcggctt tttcacaacc
    17641 tcttattctg aaaaataatt tcctggcaca accgtaatat ttttcgtttg agtatctaca
    17701 tctttgacat aaagcagtga cgtataatca tcgatggcgc gatgaccgtt gaattttgat
    17761 tcggcaaata cagtgatgcc aacaagttct gattcgaatt cttcaatcaa gctcttcatt
    17821 ccgttgactg ttccgccgcc tttcatgaaa tcatccacta caaggacacg tgaaccacgt
    17881 ttcaaacttc tcttagacag ttccatcttt tcaattcttt cagaagatcc agacacatag
    17941 ttgacactta ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat aacgaatggc
    18001 gcattcagat aataggacac agcttgggca ataggtacgc cttttgtggc tacagtcatt
    18061 acagcatcga ttttttcacc aaggtattta gaagcaatga ttcttccaac ttgacgcagc
    18121 agttcaggtt ctcctaacaa atcagagaga taaacatatc ctcctggaag cagacggtct
    18181 tgttctgata gacgttctgc tagagattca atatattttt tcgcctcttc atatgaaatt
    18241 tcagggatga acaatacacc acctgcagca cccgggattg tttccaaggt tccataccct
    18301 cgttctttaa atgttttctt gatgattccc aaatcttcac tgatagaaga tttagcagat
    18361 tcataacgtt cggcaaagct tgttaaggaa atcaattcat gcgggtgatt caataaatac
    18421 tgtgtcatgt caactagacg ttcactgcga cgtactttca cctattccaa ctccttttaa
    18481 gaaattttat cgttttacat tatataagtt tttcttcaaa aattcgagca ttttatgaaa
    18541 aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa aatttttcgt
    18601 ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg caaccaatat
    18661 tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc
    18721 tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac
    18781 acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc
    18841 gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat
    18901 agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac
    18961 gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa
    19021 tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac
    19081 gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat
    19141 caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata
    19201 ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat
    19261 ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac
    19321 atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat
    19381 cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga
    19441 aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt
    19501 ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta
    19561 tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca
    19621 tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt
    19681 tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct
    19741 aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt
    19801 ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat
    19861 gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca
    19921 atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac
    19981 ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag
    20041 acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa
    20101 cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt
    20161 tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc
    20221 taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt
    20281 cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt
    20341 tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac
    20401 ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt
    20461 gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt
    20521 gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa
    20581 ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg
    20641 atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg
    20701 cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac
    20761 tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc
    20821 tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc
    20881 agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata
    20941 catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt
    21001 atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt
    21061 tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac
    21121 taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat
    21181 gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact
    21241 acaataaaaa actgaagaat accattttag gattcctcag tttttcatca cctattttac
    21301 ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc
    21361 cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc
    21421 ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga
    21481 tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct
    21541 gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg
    21601 cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca
    21661 acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga
    21721 caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt
    21781 ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag
    21841 ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa
    21901 agatcttgac accggttttg atattgtatt tattttttag caataaagca gcttgataaa
    21961 cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt
    22021 ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca
    22081 actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg
    22141 gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc
    22201 tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt
    22261 ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata
    22321 attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac
    22381 tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc
    22441 cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa
    22501 aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt
    22561 ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt
    22621 atacaataaa cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga
    22681 cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa
    22741 cgactttttg atctttacgg acaataccga caccgctaga taatacatat gatttatcaa
    22801 atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat
    22861 ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt
    22921 tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat
    22981 caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta
    23041 cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca
    23101 taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa
    23161 agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt
    23221 aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg
    23281 tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt
    23341 cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc
    23401 ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga
    23461 ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca
    23521 agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt
    23581 ttctggataa ccacacagct aacagaatca agacatatac caatatcaac tgccaagata
    23641 acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg
    23701 cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata
    23761 gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac
    23821 tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga
    23881 cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta
    23941 aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag
    24001 gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa
    24061 cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga
    24121 ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg
    24181 aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt
    24241 gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg
    24301 tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa
    24361 gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg
    24421 taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg
    24481 ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta
    24541 ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa
    24601 actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat
    24661 catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca
    24721 tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat
    24781 ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca
    24841 gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt
    24901 cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca
    24961 ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga
    25021 gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat
    25081 ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt
    25141 gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa
    25201 gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta
    25261 ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa
    25321 tcattaacag atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg
    25381 ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc
    25441 acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct
    25501 ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt
    25561 tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca
    25621 gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat
    25681 aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt
    25741 gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg
    25801 atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct
    25861 tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt
    25921 agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc
    25981 cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga
    26041 gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga
    26101 ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt
    26161 ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac
    26221 aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat
    26281 tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc
    26341 ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa
    26401 ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga
    26461 atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat
    26521 tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg
    26581 aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg
    26641 gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg
    26701 atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt
    26761 cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc
    26821 atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg
    26881 ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg
    26941 ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat
    27001 gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg
    27061 atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact
    27121 aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg
    27181 attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact
    27241 ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa
    27301 caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc
    27361 tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa
    27421 accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac
    27481 ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa
    27541 ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat
    27601 gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt
    27661 aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga
    27721 aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact
    27781 cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag
    27841 tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc
    27901 aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct
    27961 gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta
    28021 gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc accgctttta
    28081 tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa
    28141 ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact
    28201 ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt
    28261 acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga
    28321 ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt
    28381 gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac
    28441 ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact
    28501 gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga
    28561 ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt
    28621 tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt
    28681 ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca
    28741 tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca
    28801 agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata
    28861 gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga
    28921 tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc
    28981 ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat
    29041 cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg
    29101 atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa
    29161 atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg
    29221 gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc
    29281 acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa
    29341 ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca
    29401 gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca
    29461 gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg
    29521 tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct
    29581 tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt
    29641 ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg
    29701 accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca
    29761 gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca
    29821 ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa
    29881 gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga
    29941 ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct
    30001 tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca
    30061 tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga
    30121 gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg
    30181 atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct
    30241 agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata
    30301 tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa
    30361 aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat
    30421 aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag
    30481 agttagacgg caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca
    30541 caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat
    30601 aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca
    30661 gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca
    30721 tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga
    30781 caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag
    30841 tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc
    30901 ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa
    30961 ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat
    31021 ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta
    31081 atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc
    31141 gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat
    31201 tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt
    31261 cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat
    31321 tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg
    31381 gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt
    31441 gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa
    31501 acataggcat tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg
    31561 gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct
    31621 gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac
    31681 tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta
    31741 agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag
    31801 cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc
    31861 caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt
    31921 tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg
    31981 actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg
    32041 taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt
    32101 tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc
    32161 cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt
    32221 ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca
    32281 tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt
    32341 cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt
    32401 aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta
    32461 gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt
    32521 caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
    32581 ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact
    32641 tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc
    32701 ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    32761 tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa
    32821 tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca
    32881 actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa
    32941 tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag
    33001 gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg
    33061 atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg
    33121 gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc
    33181 aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg
    33241 actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat
    33301 tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc
    33361 gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt
    33421 ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt
    33481 acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg
    33541 ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt
    33601 tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata
    33661 ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg
    33721 atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt
    33781 agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg
    33841 atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg
    33901 atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc
    33961 gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca
    34021 ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc
    34081 accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg
    34141 aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt
    34201 ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc
    34261 atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat
    34321 ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc
    34381 cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag
    34441 cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc
    34501 atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc
    34561 atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata
    34621 ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc
    34681 aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt
    34741 ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac
    34801 gatattcact tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa
    34861 tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat
    34921 gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat
    34981 cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa
    35041 ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa
    35101 atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt
    35161 aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg
    35221 atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc
    35281 tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa
    35341 ctttgtctga tccggataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg
    35401 tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt
    35461 gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt
    35521 tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt
    35581 tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg
    35641 atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag
    35701 cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag
    35761 ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg
    35821 ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct
    35881 tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag
    35941 gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg
    36001 ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag
    36061 cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc
    36121 ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag
    36181 acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct
    36241 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    36301 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    36361 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    36421 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    36481 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    36541 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    36601 agcttgtcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    36661 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    36721 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    36781 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    36841 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    36901 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    36961 aaaacaccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    37021 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    37081 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca ttataaaaaa
    37141 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    37201 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    37261 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    37321 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    37381 aataaaagat gctgaatcgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    37441 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    37501 gcgttctgct tcctcaacag agaatagatt tgattgctta taatgctttt ataataactt
    37561 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    37621 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    37681 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    37741 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    37801 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    37861 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    37921 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    37981 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    38041 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    38101 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    38161 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    38221 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    38281 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    38341 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    38401 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    38461 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    38521 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    38581 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    38641 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    38701 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    38761 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    38821 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    38881 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    38941 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    39001 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    39061 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    39121 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    39181 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    39241 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    39301 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    39361 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    39421 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    39481 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    39541 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    39601 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    39661 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    39721 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    39781 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    39841 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    39901 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    39961 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    40021 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    40081 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    40141 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    40201 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    40261 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    40321 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    40381 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    40441 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    40501 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    40561 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    40621 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    40681 catatgatcc atgactcttg gatgcagtgt agcaggctcg acattcaacg ctttcgtatc
    40741 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    40801 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    40861 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    40921 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    40981 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    41041 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    41101 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    41161 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    41221 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    41281 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    41341 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    41401 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    41461 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    41521 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    41581 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    41641 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    41701 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    41761 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    41821 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    41881 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    41941 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    42001 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    42061 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    42121 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    42181 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    42241 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    42301 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    42361 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    42421 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    42481 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    42541 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    42601 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    42661 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    42721 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    42781 cgtttttgta tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    42841 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    42901 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    42961 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    43021 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    43081 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    43141 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    43201 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    43261 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    43321 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    43381 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    43441 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    43501 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    43561 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    43621 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    43681 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    43741 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    43801 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    43861 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    43921 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    43981 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    44041 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    44101 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    44161 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    44221 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    44281 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    44341 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    44401 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    44461 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    44521 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    44581 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    44641 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    44701 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    44761 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    44821 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    44881 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    44941 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    45001 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    45061 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    45121 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    45181 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    45241 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    45301 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    45361 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    45421 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cgattttgag
    45481 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    45541 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    45601 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    45661 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    45721 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    45781 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    45841 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    45901 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    45961 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    46021 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    46081 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    46141 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    46201 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    46261 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    46321 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    46381 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    46441 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    46501 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    46561 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    46621 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    46681 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    46741 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    46801 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    46861 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    46921 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    46981 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    47041 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    47101 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    47161 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    47221 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    47281 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    47341 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    47401 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    47461 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    47521 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    47581 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    47641 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    47701 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    47761 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    47821 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    47881 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    47941 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    48001 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca
    48061 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    48121 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    48181 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    48241 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    48301 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    48361 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    48421 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    48481 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    48541 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    48601 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    48661 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    48721 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    48781 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    48841 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    48901 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    48961 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    49021 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    49081 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    49141 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    49201 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    49261 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    49321 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    49381 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    49441 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    49501 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    49561 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    49621 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    49681 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    49741 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    49801 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    49861 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    49921 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    49981 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    50041 cagtctcaca acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca
    50101 accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga
    50161 cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt
    50221 gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc
    50281 tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa
    50341 agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag
    50401 caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga
    50461 tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata
    50521 agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa
    50581 cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta
    50641 attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat
    50701 tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac
    50761 cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg
    50821 ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac
    50881 tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat
    50941 acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag
    51001 tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga
    51061 tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca
    51121 atgttttttc aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt
    51181 gactaacatg taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc
    51241 agcaatcgtt tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt
    51301 agctgctaca actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat
    51361 atctatttga ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta
    51421 ttttacatgt aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa
    51481 ataagacgag aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt
    51541 tatccaaacc acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc
    51601 atcacagtca gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt
    51661 tggacaaaga cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc
    51721 ttgtagagga atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag
    51781 caaaaatcta tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta
    51841 gagaacaaca gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc
    51901 tagacaagaa acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc
    51961 agcaggaact ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag
    52021 ggataaacag aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag
    52081 caatacagtt ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc
    52141 actatttttc tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt
    52201 caaataacag tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc
    52261 gggccaacga tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc
    52321 atataagcag agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac
    52381 atacatgtca ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg
    52441 ctttctggat ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag
    52501 acatccaaaa tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa
    52561 tgaagatctt gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac
    52621 atcattgttg taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt
    52681 aaatttgtca cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat
    52741 tttctgccct tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg
    52801 agaagtaaac aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt
    52861 tgccatatca ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa
    52921 aagcaaagca atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt
    52981 caaagtttcc tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa
    53041 ctctgtttgt ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt
    53101 ttgctgatat acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg
    53161 gatatgttct aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt
    53221 acctaaagaa aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg
    53281 gacattttgt cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa
    53341 acggatttgt ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc
    53401 taatgttttc gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga
    53461 caaccgatcc ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc
    53521 cgcttgtttt ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga
    53581 ctctgcttct cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata
    53641 attccccgaa aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc
    53701 atctagaaaa tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc
    53761 ttcaatcagt cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat
    53821 cccttgttca taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct
    53881 ccatttccgc tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt
    53941 taggtccttt gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt
    54001 ttggattttc ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt
    54061 accgacacca ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc
    54121 aatagatagt tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa
    54181 attttccata tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa
    54241 gagcagaaaa atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat
    54301 aactatgact aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa
    54361 aatggacata ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt
    54421 gttattcaaa agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt
    54481 tattaggtta ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc
    54541 ttttattcga acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct
    54601 accgcttttc ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat
    54661 gcctctgcca actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca
    54721 cgcgcaacta acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc
    54781 atttcttctc ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg
    54841 acttcttttg gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg
    54901 taacattgca tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta
    54961 ttgaaacgga tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag
    55021 cgccatggtt gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc
    55081 atttcttcgt gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc
    55141 ataattgttg aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt
    55201 tatattgcaa agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac
    55261 atcgctgaaa gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc
    55321 gattttcttc tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt
    55381 tctctgacgc gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc
    55441 ttgatcaatc tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag
    55501 tctcatcatg atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac
    55561 gattttttga tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat
    55621 aatttcctcc tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa
    55681 aaagaaaaaa gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat
    55741 agagaaaaga aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa
    55801 aaccaagatt attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt
    55861 tacaccgcca aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt
    55921 ggatcagtat gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt
    55981 tgagtaaacg gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca
    56041 tgaaaatctg tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac
    56101 ctctttttaa taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca
    56161 atctctttcc ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa
    56221 tagtcgataa attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc
    56281 gatttttgtt aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta
    56341 aggattgtat aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc
    56401 atattcccag cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg
    56461 ttctcctgta tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc
    56521 atactgtgtc agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc
    56581 agtggcataa cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt
    56641 ccatgcaccg gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac
    56701 gttatcttgc aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat
    56761 ccattgccca ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc
    56821 tttaatccca aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact
    56881 ttcaacgatg gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact
    56941 ttgtgcagtt gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc
    57001 ataaaaagca tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt
    57061 caatgtttgc ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg
    57121 agcttctgct gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc
    57181 ttcggttgaa attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt
    57241 ttcaattcct gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc
    57301 agctagcact gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa
    57361 attctttgct ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt
    57421 aactaaaaaa gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat
    57481 aaatttgctt tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct
    57541 taaattgatg gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt
    57601 atggattata cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct
    57661 ggcgtttcta tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct
    57721 ttttgcgctt catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca
    57781 gtttgaacag ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga
    57841 cgtaatcctt ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca
    57901 ggataaacaa catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt
    57961 tccatgattg gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc
    58021 atattttgcc attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca
    58081 ttattgtaca atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg
    58141 acttgtgcaa aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat
    58201 tcttttattg cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt
    58261 tctgctaata gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc
    58321 actttttctg ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat
    58381 actcactatc tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt
    58441 ttggcaggga ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc
    58501 gctgcaacaa tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg
    58561 atccaagcat ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga
    58621 tcggttagat ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct
    58681 aaacagatgc cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct
    58741 atactaatat gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct
    58801 actttgtcac taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca
    58861 gtattcagcg ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag
    58921 agcggatccg ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt
    58981 ttgctccctc cttttatcct tactttataa cggacggctt atcatgtcta atacttataa
    59041 aacatactta actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg
    59101 gaaaaaatgg atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc
    59161 agccgagcag caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat
    59221 ttagaagaag aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg
    59281 gattctggtc gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg
    59341 gcgaacaatt taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa
    59401 actaaagcaa tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat
    59461 atccagtttc atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga
    59521 ttacttgatt ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga
    59581 ctgccagccc aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa
    59641 accgtcatcc atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca
    59701 gtggacagtc aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga
    59761 acatataact tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta
    59821 gctctagatg ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg
    59881 ccattacaag cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct
    59941 cgtaaatttt tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct
    60001 ttcagctttt taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt
    60061 tcttaattta tttcttcctt ttatttctga taattccagt aaattactat aattataaaa
    60121 aaagaattga caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt
    60181 catgtgacta gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg
    60241 tttttcgtgt ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa
    60301 tcttcaaaaa gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg
    60361 tagatgacgg tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa
    60421 gaaatgattt gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc
    60481 aaatcaagtt gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa
    60541 cgatcattga tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg
    60601 ctttgaccga ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca
    60661 acaaactttt aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg
    60721 cagtagagta tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata
    60781 tcgcaatcca tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg
    60841 aagtgacgat tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc
    60901 attcagaacc attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac
    60961 aacgaaccca tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac
    61021 gaaaataccc agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat
    61081 accagctcgc gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa
    61141 gaagcgcaat cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata
    61201 tctacaacga atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt
    61261 actagtaggg atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt
    61321 acaagctggc ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc
    61381 actagggatg tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga
    61441 agttccagta aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga
    61501 atcagttgat ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt
    61561 gattgcggca gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt
    61621 ctttagcggc aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc
    61681 agtgctactg ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat
    61741 tttgagttta ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc
    61801 tacaggattg catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga
    61861 tattggtaaa tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg
    61921 atttttccca atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc
    61981 acgtccagaa aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt
    62041 ctttactggt gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata
    62101 tgtcttgcat gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac
    62161 agcaggattt gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat
    62221 tgcgaatcag ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt
    62281 tggctttaac tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga
    62341 tctttcagat gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag
    62401 caaaatctac gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac
    62461 acgccttcgt ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac
    62521 tggcgtacct ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga
    62581 agtacaattc gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc
    62641 acaaacaaac accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat
    62701 ttacgctatc gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc
    62761 ggaaaaaatg atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc
    62821 aatcgctggt acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga
    62881 tgctggtatc gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc
    62941 attcacttta tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt
    63001 tgatcttgcc gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa
    63061 tggcgacaaa gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa
    63121 aatcggctct gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata
    63181 aacaaacagg agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg
    63241 ttctcctgcc aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt
    63301 ttatttgact cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata
    63361 ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct
    63421 tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag
    63481 gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct
    63541 tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata
    63601 atttctaact ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt
    63661 agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca
    63721 gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg
    63781 aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta
    63841 ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga
    63901 tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa
    63961 gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct
    64021 ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct
    64081 aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt
    64141 tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc
    64201 agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact
    64261 ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca
    64321 taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta
    64381 ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac
    64441 ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg
    64501 taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca
    64561 gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga
    64621 aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc
    64681 gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga
    64741 aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa
    64801 aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca
    64861 tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt
    64921 catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata
    64981 aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc
    65041 tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat
    65101 tatcatggtt tattgactac cggtatccag atttctccca tatatttccc gttggtaact
    65161 cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa
    65221 acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa
    65281 gcgcttacaa caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca
    65341 tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact
    65401 gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata
    65461 actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc
    65521 ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt
    65581 gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat
    65641 gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc
    65701 actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag
    65761 atctcgggag cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc
    65821 ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg
    65881 tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag
    65941 ccaatcatgt gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa
    66001 acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc
    66061 tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga
    66121 acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc
    66181 gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg
    66241 tatctactga tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct
    66301 tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga
    66361 tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca
    66421 ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt
    66481 ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct
    66541 ttaagcatat ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat
    66601 tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga
    66661 aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac
    66721 ctttagaaaa ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc
    66781 ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac
    66841 tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt
    66901 ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa
    66961 aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag
    67021 aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag
    67081 ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga
    67141 actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct
    67201 atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag
    67261 tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt
    67321 gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg
    67381 cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg
    67441 tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc
    67501 cgcactttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata
    67561 aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa
    67621 cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgcttattc
    67681 agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa
    67741 cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt
    67801 tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt
    67861 tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactct ataaaaatcc
    67921 tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac
    67981 taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca
    68041 tcctaaatcc acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat
    68101 gaagtaaaga agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt
    68161 tgttttataa gtcatgacaa atttccttcg ttgctggata gattttccgg cattgttgat
    68221 agattttatg atacttttct acattttgta agagaggttc cgctgcatct gtatattgga
    68281 taaaattctg acaacaactt tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca
    68341 ttgttgcgcc taatgcaggt ccttcctcca cacttaaaca atagaccgtt gcatcgaaaa
    68401 tatcagcttg gatttgtagc catgcaagat ttttcgcgcc tcctcccacc gaaacaatac
    68461 gtgtaaatct gcgacccgct agttctttac gtttcaatcc caccattgac aaatgctcat
    68521 taaaaattta gaatattcac aagtttaacg acaattatga aaatttatgt cgtgctaaag
    68581 gagttttgtt attggatgaa aagttagata taaaaactgc gatcaaagac acgttaccta
    68641 ccgttttcgg ttatatcggt attggacttg catttggtat cgttgggaaa gctgccggat
    68701 ttcatccact agtcgtcacg ttgatgtccc tgctggtcta tgctggttct gcccaattta
    68761 tcacagtcag catgcttgct agtcacagcc cattgctttc catcgttttc tcgacctttc
    68821 tagtcaattc ccgaatgatt ctgatgagta tgacgattgc tccttatttc aagaaaaatc
    68881 ggctgcttca aaatctgctg attggcacat tgctgacaga tgaaagtttc gcactgggaa
    68941 tgaataaatt gaactatact ggacaaaagt taaactttcg ttggatgaat acagctaatt
    69001 ggatttctta tttgacgtgg gtgggttctt ctcttgttgg cgcactttta gggaatttta
    69061 ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt atcgggttac
    69121 tttatcttca agtcatttct gatagaaaca cctctaaacg attacagttg atcctgattg
    69181 gtctcacttt gattctggta tatgtcggac tgatttttat cccaagcaac ttagtgattg
    69241 tagttgtaac actgattggg tgcggattgg gggtgtggat caaacatgcc ttcttttgag
    69301 tttacattgc tgactatttt aggttgtact ttagcaactt ggctttctag ggtcctacca
    69361 tttgtcttac tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt aagttttgtc
    69421 ccgatcgtca ttatgtctgc tttatggttc agcagtctat ttacccaaaa tatcggacat
    69481 ctgccgcaga tcaatctcga aaatgctttg gcttcagttc ctacactttt agctgctatt
    69541 ctttcaaaaa gcttattggt gatcgtcttg gcaggaattc tttccctttc gttgattcgc
    69601 ttattataaa taaaaaaaca agagcttatg gcaaattact gtcatgcgct cttgttttta
    69661 tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta tccgttgaac ggaccactaa
    69721 caacgacttt tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct
    69781 tcgcattcat cccatctgaa tagaccttgc tcaatgtact gcggataatc ccttcatttg
    69841 ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc gatttcatag ttatagtctg
    69901 ttttagcgaa catatactcc cagtcgaagc tgatagattg gtttttccaa acacctatat
    69961 ctaacggctc tttgatccct acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct
    70021 tgatttgatc aaagtagcgg gtaatatcga ataatacagc gacatactct acttgatcga
    70081 ttcctaattc acgaaaactt ttacttaagt cgtgatgata atcgattgga tagtctacac
    70141 ctgttttttt cagccattca aaattcttcg gacttgcagt cgcgtagact tctaggccag
    70201 cccaatgcgc tagttgattc aaaatcgaac cgacgccccc ggatccgttg atcactaaga
    70261 tttttttccc gcgattggca ttttcttctg gagttagacc aaatttttca aataacaatt
    70321 cataagcggt caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa agattttttg
    70381 gtgcaagagc gacaatacgt tcgtcaacta gttggtattc ttgattgctt cctgctcttg
    70441 ttgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta
    70501 cagcaacaac tttcccgact ccatcaaaac ctaagatacg taaagcattt cttatcccgt
    70561 tatcttggcg aagtttcgta tcgacaggat taacagatac tgcgttgact tttaccagaa
    70621 gatcccgtga actgggtaca ggtatctgtt ctttttcatc aataaagctt tttggatcat
    70681 cgatcggcaa cccttcataa aaaccgacta ccttcattaa ttccttttct gacatacgaa
    70741 ttcctcctta gcttcatcaa gttggagcat ccatacagtg ttcatttcaa tatatatagt
    70801 atgctatttg ttccaccttt tttattcatc ataattattc tcttacttaa agtaaactat
    70861 ttgcactttt ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttataaa
    70921 cggtgttctc ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac atgagaagct
    70981 gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctgatat
    71041 aaacggtgtt caatcaactg accctcggta ttagctcaaa aaacgacaaa atatgaagag
    71101 ctatttttgt ttggctgttc ttattacttg atacaggaca gctttttcac gacctctaat
    71161 tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa
    71221 ggtctctaag atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa
    71281 gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat
    71341 ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc
    71401 gatcagttct ttttctgtat gttccctcgg gtctttttca aatgcataag gattcttgaa
    71461 aatccctcgg ccaatcatga tgccatcaac gccatatttc tcggccaatt cttctcctgt
    71521 ctggcgatcg gggatatctc cattgatcgt gattagtgtt tgtggagcga tttggtcacg
    71581 taaagcaatg attttaggaa tgacttccca atgtgcatct actttactca tctctttacg
    71641 cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc
    71701 ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc
    71761 agctttagct gcttcaatca attgtgccgc tacttctgga cgaaggatca agccactgcc
    71821 ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa
    71881 gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat
    71941 atgggcaacc atcggctgtt catcttctgt aaatgttaat cttcctcgta cactgtcttt
    72001 tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc
    72061 tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa
    72121 aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc
    72181 cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa
    72241 caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaaagact
    72301 gtgacaaaaa tcttatcaca gtccattttc cggaataaat ggtgttcaaa agcttgcatt
    72361 tgcggtaata agtccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt
    72421 attacttgat gcagaacagc tttttcacaa cctctccact aaacggtgtt caatcagctg
    72481 accctcggta ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc gtttgtttct
    72541 ctaatactca ggtcaaaacg ctgatttcac aacctctttt ctaacttctc aatcctcttc
    72601 ctttattgat caaataccaa gtaacggcgt acaaaacaac gatgaataac acaagtacga
    72661 tcatcgatgt tgctactggc acatcgatcg ttccaaggaa tccgtaacgg aaaccggaga
    72721 tcatataaac gatcggattg acttttgaaa cagcttgcca aaagggcggt aacatagaga
    72781 tggcatagaa aacgccacct agataagtta acggctgcaa gacaaatgta ggcacgatcg
    72841 atacatcatc ataagactga gcaaaaatcc cgttgagcag tcctgctaat gaaaacaaga
    72901 ttgccgtcat caataatgta atgatgacca tgaaccatga atacacatga agtggtacaa
    72961 agaacaagga aatgatcgtt acgagagagc cgacgagaat actgcgaccc acaccaccaa
    73021 tcacaaagcc ccaaataatc acatgcgtag gaacaggtgc taccaagatt tcttcgatat
    73081 ttttctgaaa tttttgagag aagaaggacg aagaaacatt ggcatacgaa ctagtgatag
    73141 cagacatcat aatcaaacca ggaacgatga attccatata tgaaaaacca cccatgtcac
    73201 cgatccgccc accgatcatt tttccgaaaa taacaaaata caaggatgtc gtgatcactg
    73261 gcggaaccag tgtttggacc catatacgca aatagcggtt cgtttccttg atagctaaac
    73321 tttttaatgc agtaaaataa agattaaaca tttttctcct ccgtatgatt attttcttct
    73381 gtgattttca agaacagttc ttctaaacga ttcgacttat tcctcataga aagaactttg
    73441 attccttgtc ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg
    73501 acagcgattg tctgctcatc ttcaaatgta caagcatatc cttcaatcac aggtttggta
    73561 tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca gttttgacag aagctctttc
    73621 atacttgtat tctcgatcag ttcacccgat tggatgattc cgatgttgcg acaaagcatt
    73681 tcagcttctt ctagataatg cgtcgtcaaa atgatcgttg taccattttc gttcaattct
    73741 cgtagaaact cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt tggttcatcc
    73801 aagatcagca aacgtggttc atgcatcaat gccctagcaa tcatcagtct tcgcttcatt
    73861 cccccggaaa gcatacgtgc acggacattt ctcttttccc aaagattgga ctgcttcaaa
    73921 tatttttcgc tgcgtttcat cgcttctttg cgagagacac cgtagtagcc tgcttgattc
    73981 acgacgattt gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg aaccaatcca
    74041 atctgttgct ttgctcgaac tagatccttg tccagatcat aaccgaagac attcactttt
    74101 ccagatgttt tattgactaa agaagtaata atcccgatcg ttgtagactt cccagctcca
    74161 tttggcccta atagcgcata gaaatctcct tctttcaccg aaagatcgat tccccgcaat
    74221 gcttcgaccc cacttccata gatctttttc aaattattta tttcaagcgc atttgccatt
    74281 tatttcctat ctccttatag acatgattct taattgtatc atggaataca gcagatgaaa
    74341 aaacaacttg gcttccacac aaaaacacac ctgctaacaa agaggagatg tgttttctag
    74401 ttcatgatca tcttcgatag aataactatc taaacaactc tggaactttt aaaatcataa
    74461 aagtaccgac agcaactact acgatgacat atagaagatc cttataactg aggttcttgt
    74521 cttttttatt catgttaagc tttcctccgt ccttttatcg aaagcttttt tcttttattt
    74581 taaccgaaat aaataaaatg gaaatagtta ttttcaattt ctgttttcgc tgtttgtttt
    74641 acggagaact ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt ttcgataaaa
    74701 gctgctagaa ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa tcttccaaga
    74761 tcatctggac agcagactcg ttcccattga attctgccac ttttttggca tcaatgtact
    74821 tcttttgata atcgtctttt tggacccacg catcatgctt cgtttgataa agttaatcaa
    74881 acggatacca ctgacgctgt tcatcgaaca acaaactttc tttttgggcc tgttcaacta
    74941 tggatttagg ataattcaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc
    75001 cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt
    75061 ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa
    75121 atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt
    75181 cgatcgtatt tgttcctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag
    75241 ccacttggtc cagcaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt
    75301 caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact
    75361 gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga
    75421 catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga
    75481 ctggattaac taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc
    75541 cctcagtaaa ctctggtcgg caagtgatcg gcatataggt gcgataattc aaaacggctg
    75601 ctgcaacttc caattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt
    75661 ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat
    75721 attcaaaaat caattctgct tcactcccgc tcttcattcg aaatgaaaaa cctaatttag
    75781 ggatgtctct taatggttta gtttgctgtt tcagctcttt ttgaagtggt gtcgtcattt
    75841 ttgctaactg attggcggct aaaatcgttg aaaccaaata actcatgcta ttcagctctg
    75901 tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaag ccaccgattg
    75961 ctagatagat tcctagtact tgccctaaaa gaagcaaacc tacacccacc agcggaagca
    76021 agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat
    76081 attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat tggattcttt
    76141 ctctcagttg aggattttcc tgataaaaag caattagttt ttctaactga taatcttctc
    76201 caaaatcaaa gctacgtaaa cgctgataca acgcttctga acctacacta gaataggtac
    76261 tgttgatccc ttcaaatact tcgaacatat ccaagtcata ccaagtaaga tcgtcgattt
    76321 cactatccca tgaacggaat tttttctccg tgagccacgc atccttcaaa ctttcttctt
    76381 tatcaaatct cggttgataa ggaatttttc cccaattact acgaactgtt tcctttactt
    76441 tcaaacgtga aaaaatgtct aaaacaacta ataaaataac aactgcaata ataattaaaa
    76501 cgataaattc tctttggccc atatccctct ccgttccttg atttgttagt tttagtatag
    76561 cactgttgcg ctatttgaaa agggggtaca aaaataactg tcttcgttac ctaattcgta
    76621 tcaatcacac caaaataaaa acaccttcca aaaaagaaga tgtctttaaa acgagataca
    76681 attttttgtc attcacttca ggtaaattag agccaattaa accctccgca acttgacgga
    76741 gatggagaag atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa
    76801 tttttttgac aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc
    76861 ggtataaaaa cgctggaaga ctttttcaac agaggtttgc gctcctggtt tgataggatt
    76921 ggccactgtt aatcgaatga atgaatcctc ttttgaaaat gtcacctttg cttgtccatc
    76981 tgcataacgc aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc
    77041 tgctaataca gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga
    77101 aaattgttca taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg
    77161 gagatccatt tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat
    77221 actgagtaat cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt
    77281 atcctctacc tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag
    77341 atcatgagaa aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg
    77401 tcctttttgt tcattttcat ccacccatgt attcagtgca tctactaggt ccattagatt
    77461 cttttctcga aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc
    77521 gattttctta gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa
    77581 gataacaacg agaagcaaca gccacttaat caaatctaaa ccccactccc cacaccgtat
    77641 gaatatacac agcatgtgcc tttgctagtt tccctcgaag attggaaata tgcatattca
    77701 ctgttttctc actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg
    77761 aaaatacttt tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca
    77821 aatggacagc cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct
    77881 tgtaatagat gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga
    77941 tgccgattcg taacaacagt tcttcgatat caaatggttt cgtcacatag tcatccgctc
    78001 ctgctgccaa caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg
    78061 gaacattcga tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca
    78121 tgagatctag aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac
    78181 ctgaataagc atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaagaatgaa
    78241 cagcttcatc gtcttcgata atcaaaatag tttccagcag gaacacctac ttaatgtcag
    78301 atttttggaa aataaacatt cctagagctg atgataccac gattgttact agactgacca
    78361 gaatcatttt cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc
    78421 acgtttccgt aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg
    78481 gaacgaaggc cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc
    78541 tagtgatgaa tgtcaaagcc actggaacag ataatgtcgc taatatcagc agaagcaata
    78601 caggaaatac ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc
    78661 cgaaagcaag tgtgtaagca atcatggcta gccccgtaat caaaaccacg ccaagtgttg
    78721 tgatcaaaag cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag
    78781 ataacgtatt gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg
    78841 ctgcgaaagc cagaagaatc catgcagcca ttttttccac cattaaaatc cctgttgcac
    78901 cgttgatgac tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc
    78961 attcgttcca agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt
    79021 tacggctctt ccatactttt attttttcag cagttaatag attaaacatg tggattgcct
    79081 ccagttaatt gtaagaaata atcttccagt ttttgcccaa ctaggtggat accatccaca
    79141 ggaacttttg cctctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa
    79201 agctggataa cacggctaga ttggacaaaa taatccctat aaccaagaga gtcgagaacc
    79261 gttactgctt gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc
    79321 aattcttctt tcgataattc tttgatcaag tgtccttcat gcaaaatacc gtagtgagtg
    79381 gcgatctgtg acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct
    79441 tttaccaaac gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc
    79501 gtcggttcat ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa
    79561 cgctggcgca tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc
    79621 atcattttca gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata
    79681 cgtaggttat ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc
    79741 atacgtcgtc ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca
    79801 atcgagatca aacccataat agttctcata aaggtagact tacccgctcc attctcccca
    79861 atcaaaccat agatcattcc acgtttgatt tcgatcgata ctttatctaa tgctttggca
    79921 gcaccgtatt ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata
    79981 cttatccttc tttcttattg ataagtgaag tatagttgac gggtataaag aaagcagcca
    80041 gaaatgtata aagaaagtgt aaattttcca attgaatttc aagaaaaatc agactgtctc
    80101 gcagacgtgg ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct aatccaagtg
    80161 gactggccac catggtaatc gttgttatcg ttgacatcag acgtccttgt atatgcgttg
    80221 gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac
    80281 ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt
    80341 tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc
    80401 ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt
    80461 atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga
    80521 tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc
    80581 tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag
    80641 aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac
    80701 tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt
    80761 tcttaagacc tggttttttc ttcgtatcat tgaataagtt tgaaaagcct tgatttataa
    80821 aggtttttcc gtaaatatat tttgttatat ttgattaaat ttgaaatgtt tgtgttctaa
    80881 aatgtgttct aatttaaagg cagtatattt cgtattttat attaaatttt taggctcatt
    80941 cattctcaaa tccattctat ttagtcaata aatattttaa aaagacttta taaacaaaga
    81001 tgttcaagcg agcatcttat ttttttgtgc ctaatttgaa aggagaacat ttcatgtata
    81061 aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt caatcaattg
    81121 taggagcaat tgctcctacg attgcttttg ctgatgaaat tacccatcca caaacggtaa
    81181 ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt gatggtcgtc
    81241 caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggcgttaaa caagatattt
    81301 tctgtgttga accaggagtc ccgatttaca atgagttcac tcctggatat gagaagaacc
    81361 cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa aaagtaggta
    81421 cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt aatgggtata
    81481 cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc attgaggcaa
    81541 aaatcaatca agccattgcc gattatcaga agaaaccaag cttccataat agtacggcta
    81601 aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat ttgtcagagt
    81661 tcgatgaggt tgtggaaaat accgcaaaca ttgattatcg tgtgaatggc aatcagttga
    81721 taatcagccc aaatgccaat tcaaaagaaa gtggtgtgtt gacgcttaag aaatctgctg
    81781 gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg gccggggcga
    81841 tcgataagcc gaacacttat acggtcaaaa ttgatgtgga aatggagggt tctttgaaga
    81901 ttaaaaaagt tgataaagaa tcgggtgata ttataccagg aacggttttc catttagact
    81961 ttggaaaaac tttacccgca aaagacgtga ccaccgataa agaaggcatt gcgaccttag
    82021 atgagattcc tcatggtaca aaggtaacca ttactgaaaa atcggtgccg gcgccttata
    82081 cgattgatac cacacccatg actgccacaa ttaaagcagg tgaaaccatt tcagtgactt
    82141 caaaaaatac gcgagaaaaa ggtcaaatta ttctggataa aaccggaatt gaaacaggga
    82201 gtgatctttg gaacgataat tactctttag cgggcaatac gtttgccatt cgcaaggata
    82261 gcccaactgg tgaaatcgtc caagaaatga ccacggatga aaaaggtcat gcagaaacac
    82321 caaaagagat tgccaatgcg ttggaattgg gaacctacta tgtgactgaa accaaggcta
    82381 gtcatggctt tgtgaatact ttcaaaccag taaaagtcga attgaattat gtcaatcaaa
    82441 cagtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaattact ggtgaaacca
    82501 ctttgacaaa agaagacaaa gataccggtc atgaaactca agggaaagct gagtttaaaa
    82561 gagctgaata caccttgttt actgttaagg atggaaaagc ggtcaaatgg accgagaact
    82621 ttaaaccaga attgttgaag ggaacgaaag cttctgatga aaccgtaact ttgactttag
    82681 atgaaaagaa tcaagttgcc gttaaacact tagcgattaa tgagtattac tggcaagaaa
    82741 ccaaagcgcc tgaaggatat accttggatg aaacaaagta tcctgtatcc atcaaaaagg
    82801 tggatgatag cgaaaaaaat gctgtgatta ctcgagatgt tgcggcaaaa gaacaagtga
    82861 ttcgctttgg ctttgatttc tttaaatttg ctggatcaac tactggcact gccgaaactg
    82921 gcttcaatga tctaaccttt aaagtgtcgc cattggaagg aaccaatgaa atcaccggtg
    82981 ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt tatggcaagt
    83041 ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca gaaggttttc
    83101 aaaagattac gccattagaa atccgttcta cttttaaaga aaataaagag gattatacca
    83161 agagtgatta tatctttact attaccgaaa aagggcaaaa gcaaccgatt aaaacggtaa
    83221 cggtccctta tgggaatcta accaatgaga atttctcagt cagtttgaac cgtttgatgc
    83281 tttatgattt gcctgaggaa gaagatagct taacttctct tgcgacttgg aaagaaggag
    83341 ataaagaatt gactactctt gattctaccg agctagtcga taaattgagc tataatctac
    83401 atgaaatcaa agaagactgg tatatcgtag ctcaagccat cgatgtagat gctacaaaag
    83461 ctgcccaaga aaaagataaa aaagccaaac cggtggcgat tgcggaaaca accgcaactt
    83521 tagcgaataa agagaaaaca ggaacttggg agattctgca taaattaact gtcgaacaag
    83581 ttttagacaa gaccattgtc ttattcaact atgtgtatga aagcaaagaa gcctatgaag
    83641 ctggtgatga gccggtagcg aaggatgcta gcttgaacaa tcaagcgcaa actgttaatt
    83701 gtatggtgga acgtcatgtt tccatccaaa ccaaagcgca cttagaagaa ggttctcaga
    83761 cctttactca tggtgacgtg gtagatatgt ttgatgatgt atccattacc catgatgtac
    83821 tagatggctc aaaagaagct tttgaaacaa ttctgtacgc tttatttcca gatggtacaa
    83881 gtaaagaaat ttggaaatct ggcaagattg attacgaagt gaatgacaaa gaatttacca
    83941 aaaccgtact tgcggaaaaa gtggatacaa gtaaatatcc agaaggtaca agttttacct
    84001 tcaaagaaat taactatgac aaagatggga atatcaatgg aaaacacaat gaagatttga
    84061 aagaaaaatc tcaaacttta acgcccaaaa aagttccgac aacaccaagt acaccagaac
    84121 aatctgaaat gccaactgtt ccaagtggct ctcaagaatc tagtcctaca gtgaaggcat
    84181 tccctcaaac cggggagaaa acttccaatg tgctactgtt cattggcttt agcttgatct
    84241 ttgcgacggc tggctattat ttttggaatc gtcggaacta aggtgataag atgaaacatg
    84301 taaatagtag aaaacaatac gcgcgaccgc ctcctaaaaa agtggtcgcg ctatttttgt
    84361 attcaaaact aacaatgaaa agaggaaaaa atatggaatt gaaatttgtt gtaccagata
    84421 tggtcgagac gttcggaaaa ctaacatacg gtggtgaagg tgaagttcta tcggaagggt
    84481 atggtcgaaa tactacaatt gttggtcgaa gttaccatct gttttcaaat aaacaacggg
    84541 ccgatgatat tgaagtggtg gtagctgcgg aagccggcga aaaggatttt gaccaagatc
    84601 aaccactaaa agctgtaaat ccccacttaa ttgccaaagg ctatgaaatt gagaatcgtg
    84661 ggtttaccga ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga
    84721 tgagattaac agaaggtatt gtcgtagatt caggattaac ctttggcaag ttgcgttttt
    84781 ccgcattacg tcgagaagta cgcaagcaaa atgaggacgg aacggttagt aacgaggtaa
    84841 aagaacggac ctataatttg aaatcctccg cgcaaggtcg aatgatccaa gtcagtattc
    84901 cagcaaatgt acccttacgc gagtttgctt atgatgcaga ggtagagctg gtcaatccaa
    84961 tcgtggatac ggtcgccaat tatgtttttc gggaaggaac caccgttaat tggtttatca
    85021 aggcagatga tttagtcttg aaacgacaac tgaatcaagg aaaacctacg aaccaaaatg
    85081 aaggaaagaa ataggtgaag aatatggaaa ttaatgttga acatatttta gattgtctcg
    85141 atcaatttgg caaaggtgag ctaacggagg aacaactcac taacgcatta acctatgatg
    85201 aaaaaatgtt tctgattatg catcaagggt tactggaagt aggtaacgat gccaaagaag
    85261 actttgatgt actggattgg ttaggggaag aagagtcttt ctttatggtt gtcgaggtaa
    85321 atgaaagttt atgtcggcag gcagaatctg ttttggaaga aatcgatgtg gagatgccgg
    85381 atgccatcga aagattttta aaacaattag tggaaacgaa acaacttccc gtagtagtaa
    85441 atgactagca taactttttg aaattgccca atgaaatcct attgggcttt ttcgactgga
    85501 ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc gcaggagatc aacacttgat
    85561 ttatcacttt gttcttggct ggctaagtgc cttattcatc ggttggatgg gcgtctttta
    85621 ttttccagag cttagacaat ttggtctttc taaggtatca ttttccacta ttgaaatcgc
    85681 ttggtccatg aaagatttag tatgtctgct tggaagtctc gctctttcag gaagtatgat
    85741 gttgctttat attcattttt ttcctgatca ttggcgaagt ttgtggaatc gacaaaagct
    85801 agccagaatg attctagaaa accattggta cgaagtgaag caaactcaaa gtgatggctt
    85861 tttcaaagat ttgaatagga gtcgaaccaa agagactatc agttacttcc ccaaagttta
    85921 ttatcgaatg aaagatggct tactgtctat tcgcgttcaa atttcactgg gaaaatatca
    85981 agatcagcta ttgaagttgg aaaagaagtt agaaagcgga ctgtattgtg agttagtaga
    86041 aaaggaactc aaagactcct atgtggaata caccttgtta tacgatatga ttgccaatcg
    86101 aattgggata gaagaagtag tggcagaaaa tgggacgttg cgattgatga aaaatcaagt
    86161 atgggcgtat gattccttgc ctcatatgtt aatcgctggt ggcacaggtg gtgggaagac
    86221 ctatttctta ctcaccatta ttcaagcact attgaagtca gatgcagaat tgtttatcct
    86281 cgatcccaaa aatgcggacc tcgccgattt aggtacggta atgcctcatg tctattctca
    86341 aaaagaagaa atttctaaat gtgtggaaga tttttacgaa cgcatgatgg ctcgtagtag
    86401 gtcaatgaaa gaaatgccca actacaaaac aggagagaat tatgcgtatc tcggacttcc
    86461 gcccaacttt ttaatctttg atgaatacgt ggcttatatg ggtgcgaatc gttttcctac
    86521 aagcatcgaa tagatgtgaa atggaagcct attgagatta tcaatagtga caactaatgt
    86581 tttgagagat ggaatgaaag ccttcaagtt gagctgaaac atccccgcta atactcctac
    86641 atgctctaat caatgaagta gattggtttt ggtatgaagc taggtgaagt cgtctgaaat
    86701 ctgcctaagt gcttgagcat aaggctgact atagggcggg cggctgaaaa agataactat
    86761 ggtaagaatg tctgaaaatg actgacgaat ctgcgaatgt acgggtctaa aaggctgatc
    86821 ttattgaaag ataggaacat acggacgtat ggtgagatac cgacaagtaa ggttccatga
    86881 tatgaaaatc gggatagcta acgaagaagc tataatttca caggctcata gtagatacct
    86941 aaggttatac atgatagcta aaaacattgg aacgatgtaa gcagaaaact atcaattaaa
    87001 tgactacttc tagataggtg catataagta ataacgaaat tgctttgttt ctgtgaaagt
    87061 aagggcacag taccgatgaa gcgtctaaca aatgtggagg gatagcccta agtctattta
    87121 ataacaatga atgttgaaac agatgaagtt caaggatctg gtaagaacgt gggaaaatcc
    87181 gttatggagg tgccacgctt gaacacaaaa atacgttact gggaatatta tgatctacaa
    87241 gaaacattcg atcagctatt tactcaaagt agaaacggga aaaagtttta tcaattatat
    87301 gagttaatca tttctgaaaa taatattctt ttagcatacc gtactatcaa ggcaaacaag
    87361 gggtcttcga caccaggaac ggattccttc acaattgata actataagga aatgaaccaa
    87421 gcagagttta tccatcttat tctcagtcat ttagaaaatt acaaaccaaa atccataaag
    87481 cgagtaatga tacctaaacc caatggtgag aaacgcccat taggaattcc atgcatgatt
    87541 gatcgaatga tccaacagat gttcaagcag atactggagc caatatgtga agctaagttt
    87601 tatgaacata gctacggttt cagaccattg agaagtgcaa agcatgctct aggtcgcatt
    87661 atgtatctca taaacatcag taaaatgcac tatgcagtag acattgatat caaaggtttc
    87721 tttgataatg taaaccaccg tttactgata aaacaattat ggaatatagg aatttgtgac
    87781 aaacaagtct tagctattct ttcaaaatct ctgaaatcac ccattcaagg agaaggtatc
    87841 ccgagcaaag gaacgatcca aggaggtatc atttctccac tattatctaa tgttgttcta
    87901 aacgacttag accattgggt ctcaaagcag tggcatacct ttgaaacaaa gtatccctat
    87961 actaaaggtt acaataaatt tcgtgctcta agagatacaa atctaaagca aggctatatt
    88021 gtcaggtacg cagatgactt taaaatcatg actaatgatt atccgacagc attaaaatgg
    88081 ttccacgctg tcaagcttta cctaaaagac agacttaaat tagatatttc caatgagaag
    88141 tctaaaatcg tcaatttgcg taaatgtaag tcggaatttt taggattcgc tatttgcgtg
    88201 aaacaaaaag ggaaaaaatg ggtttgtaat tcacgtattt ctaacaaaaa aaaggatcag
    88261 attaaagaag agattaaaca aagaatcaaa gatattcaaa agagtcctac agcacaaaat
    88321 gctctcctgt ttaatgctct cgtgctcggt gttcataatt atttcaggta tgccactcat
    88381 attaatttag atttgaatag gatgaactac gttctttcat tcactactta caaccgatta
    88441 tcaaattgta gtacaagatg ttaccccaac aaagcttccc caacttacaa aaagttttac
    88501 tcaacaacga tgaaaacgta taaagtagct ggagtctatt tgtaccctct ttgcgacgta
    88561 tctacaaaaa caatttacgg atttaatacg gaggatacac cgtttactcc gtttggtcgt
    88621 caaagattgg agcacaaaag cctacagtct cttgtgttcc aagaattaag aaaattgatg
    88681 gaatcgaaaa taccgaatag aacggttgag tatttagata gtagaatatc tcgttactca
    88741 atgaaatcag gcaagtgtga gattacaaaa caatttttgc cagcaaaagc cgttcactgt
    88801 catcactatt tgccaaaaag tcttggtgga gacgacaaat tcgataactt gagaattatt
    88861 cataaggata tccatcttct gatccataca acaaataaaa tgataatcga tcactacgtg
    88921 catgaactga aactggtgcc agagcaaata gcaaaaatca acctttatcg aaagatgtgt
    88981 aatctgcaaa acattcaata gaaaataaat agatggaacg ccgtatgagg tgaaagtctc
    89041 acgtacggtg tggagcgggg gaaaagatgg agattatatc aaattcttac ctatcgctat
    89101 agatgttaac gacaaaagaa agtgcggtga ttttgaataa gcttaaacaa atcgtgatgt
    89161 taggccgtca atctggtttc tttctgattc tagcttgtca aaggccggat gcgaaatatc
    89221 taggagatgg gattcgcgat cagtttaact tccgtgtggc tttgggtcgt atgagtgagc
    89281 ttgggtattc catgatgttt ggagaagtcg ataaaaattt ttttatgaaa cgcatcaaag
    89341 gacgaggtta tgtagataca gggggcagtg tgattagtga gttttatacg ccacttgtac
    89401 caaaagggta tgatttctta agggaaatta gcaacattgt tggattaacc gttcatacgg
    89461 aaagagaaaa taatagtgtg tagcctagga atatcaataa tcctgggctt ttttattgag
    89521 gtgatttatg acatttttag atttattcgc cgggattggt ggctttcgct taggtatgga
    89581 acaagccggt caccagtgta tcggtttttg tgaaattgat gaatttgctc ggaggagtta
    89641 taaagcaatc catgatacaa ggaaggaggt ggaaatgcat gacatcacaa gtgtatcaga
    89701 cgaatttgtt cgatccctcg gaccagtgga catcctttgc ggcggctttc cgtgccaagc
    89761 tttttcaatt gcgggaaagc ggcaaggatt ttccgatact cgaggtactt tattctttga
    89821 aatcgctcgg ttcgccactc ttctccaacc taagtttcta ttccttgaga acgtccgggg
    89881 attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgt tggatggatt
    89941 ggggtatgat gtggaatggc aagtgcttaa ctcaaaggcc tacgttcccc aaagccgtga
    90001 gcggatcttc cttatcggac attctcgaga tgcatgtacc gaacaagtat ttcctatcat
    90061 tggatcgtct tcaacatctg atcaaaacat cagaaacttg gtaaatatca atccttccaa
    90121 tcgaggaatg ggtggccaag tctatggttc agatagcgta gcaccaacct tgaccggtga
    90181 tgaaggaatt aaaattgcct taccggtaaa ggatgggatt tctgtagcgg gtatgttgtc
    90241 gggaaatttt gaacaaggca ataggattta tgaggtaacc ggaacagccc ccactttgtc
    90301 aacgaaacaa gggggaacga agattatgat tcggaaaaat gaaacagaat atcaagaggt
    90361 gaagccaggg gacagtgtga acctggcttt tcctaattcc acaagtcgcc gaggtcgtct
    90421 gggaaaacaa agtgtgcata ccttactaac aggagaccaa caagcggtag tcacggatca
    90481 gtaccagatt cgaaagttaa cacctaggga gtgctggcgg cttcagggct ttccagattg
    90541 ggcttttgat cgagcttctc aagtcaattc agatagtcag ttgtataaac aggccggaaa
    90601 ctcagtaaca gttccggtca tttttgatat tgctagaaga ttaaaggggg taaaagcata
    90661 tgatttatga cgagttgatt ggagaaatct actgggtgat tggcaagatt caggcagatc
    90721 cgaaattgga agaagaactg taccggctca attttgatat tcgcaaaaac ggggtgaaag
    90781 tccaggtgat tcgtatgtag tggatgagga aacggatgct cgaatggagc tcaatcaagt
    90841 catcggtgaa ttggaacgaa ttgcggacct tgccaaagaa cctgatatcc gccaatactt
    90901 gtttgagata aaagcagagt tggaaattga gggtatcacc gcggagtaat agggaaataa
    90961 cccactgata ggcactgacc gcgggggatt tgcgaagcag aaatcctccg tggtgctgtg
    91021 cctgttcatg gacggcggca gccggccatg atgtctcacc ccccgtatct aacagggggg
    91081 tacaaaaaca caaaaaacaa gcaagtaacc ataaaaaatg cttgttgtat caaggatatt
    91141 aagtaatttg tataggtgtt aactcatttg gaaaagttaa caccttgaat ttggaggtga
    91201 aaacttggcg caacgaaatt tagattatcg gttattaaaa gaacgacgca acgaatatgg
    91261 cgtttcacaa aataaattag ctactgcttg cggacttagc cgcccatacc taaatcagat
    91321 agaaaacggt ggcgtaatcg cctctacaaa gaccatgaga aaaatttttg atcaactgga
    91381 aagtttcaat cccgatttac ctttaacgtt actatttgac tatgtaagga ttcgctttcc
    91441 cacaacggat gtgcggaaaa ttattcaaga aattctccat ttgaaattcg agtatatgct
    91501 tcatgaagat tacgcctttt actcctatca agaacaatat gtcatgggcg atattgtagt
    91561 gatgttatct catgaagaag ataaaggtgt ccttttggaa ttaaaaggtc gtggttgccg
    91621 gcagtttgaa acttttttac tcgcccaaaa gcgtagctgg tacgactttt ttgaagattg
    91681 tctaaaagcc ggtggcgtga tgaaacgctt ggatttagca atcaatgatc gagtggggct
    91741 actggatatt cctgatttaa cgaaaaaatg tcagaaggaa gaatgtatct ccttgtttcg
    91801 taccttcaaa agttaccgtt ccggggaact gttgaaagct gatgaaaagg atggtatggg
    91861 caatacctta tatattggca gcctcaaaag cgaggtttat ttttgtttgt acgaaaaaga
    91921 ttatgaacaa tatatcaagt tagggattcc attggaccag accgaaacga aaaatcgttt
    91981 tgagattcgg ttgaaaaatg accgtgctta ttatgcgatt caagatttgt taaaaggtcg
    92041 tagcattgaa agtaccacgt tttccattat caatcggtat ttgcgatttg cggataaagt
    92101 agatggaaaa cggcgaacca attggccctt gaatgaacaa tggggtcgtt ttattggacg
    92161 gaatcggaaa gagattcaat taacttctga accgaaaccg tataccattg aacgaacatt
    92221 aaattggcta ggtagacaag tcgcccctac gtggaaaatg gctaaggagt tggatcgatt
    92281 gaatcaaaca acgtatatcc aggatatggt acagaatgcg cgactctcgg accggcataa
    92341 aaagattttg gagcaacaaa gtatggcaat cgaaaacttg attgtatgaa aagaggaatt
    92401 ttgatggaaa ttgtaaaaga tatgttattg gtacttggtg gcagtatgct tggtgttacc
    92461 gtgatgtgct tgatgaatgc aagtgcagca gcggattgag caatggaaaa gaaaggaaag
    92521 tgacaaaatg aatttcggac aaaatttata tcaatggttt ttaaccaatg ctcaatcttt
    92581 agtcttacta gcgattgtgg tcattggttt gtttttagga tttaaacggg agttttccaa
    92641 actgattggc ttcttggtga ttgctttgat tgcggtaggt ctggtcttta atgcttctgg
    92701 tgtgaaggat gtcctactca atttgtttaa ccgaattgtc ggtgcctgag ggggaatcac
    92761 aatggaacag atgcgtgttt atattgcgaa ccttggaaaa tacaatgaag gtgagttggt
    92821 gggggcatgg tttaccccac ctgtcgattt tgatgaagtg aaagaacaga ttggattaaa
    92881 tgacgaatat gaagagtacg cgattcatga ttatgaactg ccttttgaga ttgatgagta
    92941 tacgccaatt gaagagatta atcggttgtg taacttagcg gaggaactag aaggaacgcc
    93001 aattggtgaa gctgcttctg agattcaaca cgctttcttc aattctttcg aggaaatggt
    93061 agagcatgtg gatgatattg tctattatcc agattgcgat gatatgagtg atgtggcgca
    93121 ttacttgatt gtagaagctg gtgattttgg cgaagttcca gaacaattga agccgtatat
    93181 tgattttgat gcttgcggtc gagatttgga gattggagga aattatttgg ttactagtcg
    93241 tggagttttt gagtatccag aatgaatcaa acactatata tagtgtttga ttagtatatg
    93301 taagcaaaaa aacacaatat atagagtgca aactgaaggg taatgtgata taatatttgc
    93361 cgaagaaata tttttgaaat gaggtaatat tatggcaaat attcaaacaa gttttattga
    93421 tttccataat tcaattagat tggacgtaga agacaatacg ctacttaagg actataaaga
    93481 ccaagtaatt gatggattaa aagattactt acctgatgat gtgaagtttg aaactttttt
    93541 acagggaagc tattctgttt ataccggaat aaaaagctgt gatgaaaaaa ttgattttga
    93601 catcgatatt gcagttgctt tcgaaattga tcatactgtt tatgaagatc caagggagcc
    93661 taaactgtgg gtaaaagagg cgctagttga aatttttcct aatgctcaag ttaatttaaa
    93721 agtaccttgt gtaactgcta catttactgg aaaaaagaca aaaaaaaatg tgcacgttga
    93781 tgttgcggtt tatgctaaag aagatgaaaa ttatttctta gcaaaagcaa aagaattcag
    93841 tgctcccgaa aaccgatgct gggaggaagc ggatcccaaa gtattaaaag agaaaattaa
    93901 tagtcatgta gcagattcag atgatcgaaa acaatttcga agatgtattc gctatttaaa
    93961 aagatggaaa gataacaatt ttaaccaaga atataaacca acaggaattg gacttacaat
    94021 taatgtgatg gatacgttcc tagttaataa gtccactgat tttttaacta gaaaagttca
    94081 gtataatgat atggaatgta tgaagcagat tgtttcttcg ctgaaagatt catttgtgta
    94141 tgagtacagt gaaacagatg gttggcatta tcgtctacat gcatatcttc cagtgaagcc
    94201 taacagtgac acctattcaa aaatgacagt gaatcagatg agtgatttta aaaacaaact
    94261 ttctaaatta tatgatgatt tgattttcgc tatcgatact gaagatgagt atgaagctac
    94321 aaaacgatta aataatcaat ttggggagga ttttttaatt atttcagaag aagaagttac
    94381 agaaaaaaac ttaagaaatg catttgttac agactatccg agtgcttagg ttggtgttaa
    94441 agattggatt atactgatat tttaaaagaa aagctaacag atgttgtgcg tttattcgat
    94501 ggtaattact atggggattt ttcatttgct acaattggaa agttgcgttt aaaaattgaa
    94561 ataccaaaac attttccatt ggtacttcct aggatttaca ttcataattt tgatcattta
    94621 ggactttacc taccacatgt agaaaaagat gggttggtat gtttttcttc agatataaat
    94681 tatttagtag atgaagaaaa ttcagaattg gttttattgc agtgtgttaa taaaactata
    94741 aaaacaattg aggaaggttt gaaagggtta aataaaaatg attttcgaaa agagtttgtt
    94801 gctttttggg acaaacaaga gaatcaaatt tcgttattct acctttgtga accagcgttg
    94861 tttatcaagt cagttgatac aataatgtac ggtaagtctt tggttgcttt ggataaagag
    94921 aacgaattac aattaaatgc agcgagtaga ttgtttaatg gagagaaaga agatactcat
    94981 gaattagatg catactatgt tccaattaga aacaagaata atattatgcc cccgaagaat
    95041 aataaagcat ggtcaaagaa ggatatttta caaatagtaa aaaaaaatgt aacaggtgca
    95101 aataaaagat tttttttaaa acttgaaaaa agaaagaata gacataatgt tttacttatt
    95161 ctagatatcc caattgaaga aaagtcaaat aatgtcataa ttggatttct ttttacaaaa
    95221 caatctggat ctataaaaga ttataagggt acgcctagga ctgtattagt tcagcgtcat
    95281 gatgaattat atttggtgaa tagaacgagc ggagaaaact atggattggg tgctaatata
    95341 gctattgttg gggtagggtc agttggcagt aaaattgctg aaaatcttgg tccattaggt
    95401 gtgagaaaga tgacactcat agacaatgat tgttttcaag ctgaaaattt gtatcgaaat
    95461 tcacttggtg cagatagttt aaaaaatgcc cgatctcaga ataatttttt taaagttgat
    95521 ttaatgcagg aggagctcga aaaaaaattc ccataccttt gtgttgaagt ggagtcaatg
    95581 aacgttttag acttaattag tatgcaacct ggttttttca aggaatatga ctatgttttt
    95641 gtttgcatag gagatacaat ggcaagcctg gctttaaata attatttttc caaaagtagt
    95701 caaaaagttt tctattcttg gctggagcca tatggcattg ggggacacac tctatttata
    95761 aattattctc taaatcaaaa aggttgtttc aattgtcttt atacagattc aaactctaaa
    95821 aataggtgtt caaatagagc ttctctggta aaagaaggac aaatttttga aaggacttta
    95881 gcgagttgcc gctcaaaatt tataccatat aatgctattg cctcgacgga atgtgcaatt
    95941 aatgcaataa aacttttcca taaagtcaat caaaataata tgaccaatag tactcttttt
    96001 tcatggatag gagatgggag tacttttgag aatttaggat atcaatatag cgacagaaat
    96061 acttttctta atggagtcaa taagtttgaa gaaagattct tttctaaata ttgcaaagta
    96121 tgtggggagt tagatgatta aattttcgtg tgaccgtatt gtcattgaag taagggatga
    96181 agttcttaag atatttgaac aatatcggca gggaaaggat gacttgcctg aagctggagg
    96241 agttcttcat ggaaaaagac ttaagaatgg gagcataata attaccgata taacaacacc
    96301 tcaaatagga gatatacaaa aaagattact ctttaaaaaa aaccgagaaa ttcatcaaca
    96361 gctttctaat gagaaatgga tagaatcaaa taagttttct attgcattgg gagaatggca
    96421 tactcatcct gaagctgtac caactccatc aaatgttgat aagaaaagct ggagactgaa
    96481 cgtgagcaag caacatgatg atagagttta tgtttttatt atcgttggat tttctgattt
    96541 gagaatgtgg ttactgtcaa aaaaatcaga tattaggcag gctataaaac tataaaggag
    96601 tgattagttg aaatcacaaa agaatatttg gtggtggggg tttttagtag gaaaggtaat
    96661 gataccagcc cttctcttaa tttctctacc gatgattact tttttcttta ccaataaaaa
    96721 aatgagtaaa gaagccgtga tttttttctt tggagaccaa aaagctacct ttattgaaac
    96781 gttagtaaca tctcttcagc ttaatttgaa ttacattttt tcaattatta ttgttatatc
    96841 gttattgaat ttttttaaaa aaagaaattc aggaaaggtg tttaactcta atggaaatgt
    96901 gtactacaac tatttttatt ttgtattttg ggtagcagca actttacttg gttatgataa
    96961 aattcagatt gcaggaattc ctatacacat gcaatataag ttggttttga gtggaatttt
    97021 ttctgaggtg ttgccagata tatacgatga tcattacgac agtgatggaa catgtaaagt
    97081 aagcattgaa aaagaaaatt tcgatgacat cgatggatat gactctgtga acttactgat
    97141 tattgatact tacgatataa aaatgagtga attatctatg gaaaatcaaa catatcctac
    97201 gataattgtt agagggaatt caatagatgg agttcgtaaa gttaatagat ctttaatctt
    97261 ggaaataaaa aaaacaatgg atgaaattca aaaaagtgac tttaaaaaag tatttgttgc
    97321 ttcaacatct aatccaaaaa atagtatcaa tattattaat tcatcgttta gatttttcgg
    97381 tcgatcacgt aggtttaaac tttatgttct tcaaaaggat tatgcaagca atggaaagta
    97441 tagtaagaaa tatagaattt ttatttaaga aggaattttt ccttcttttt attttggagg
    97501 aaactatgaa aaaaataaaa agctacacca gtatttgggc agttgaaaaa gtcatttatg
    97561 cgattaatga ctttcagctg ccttttcctg tgacctttaa ccagatggct tggtttgttt
    97621 tatccttgat gtttgtcatt gtatttgccc atgtgcctcc gctatccatg atagaggggg
    97681 cttttttaaa atacttgggc attccgatag cggtcacttg gttcatgtcg caaaagacct
    97741 ttgacggtaa gaaaccattg ggatttttac gatcctttat cagctatcac ttacggttta
    97801 aggtcacttt tgcaggaaag aaagttaaag aacagaagaa acgatgggac gaaccaat
//
LOCUS       DACDCJ010000003        65393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDCJ010000003 DACDCJ010000000
VERSION     DACDCJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.003"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            53..685
                     /locus_tag="IV038_000195"
     CDS             53..685
                     /locus_tag="IV038_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8340448.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            808..1446
                     /locus_tag="IV038_000196"
     CDS             808..1446
                     /locus_tag="IV038_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8340449.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(1565..1637)
                     /locus_tag="IV038_000197"
     tRNA            complement(1565..1637)
                     /locus_tag="IV038_000197"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1602..1604),aa:Thr,seq:cgt)
     gene            1758..2000
                     /locus_tag="IV038_000198"
     CDS             1758..2000
                     /locus_tag="IV038_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340450.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2139..2768)
                     /gene="upp"
                     /locus_tag="IV038_000199"
     CDS             complement(2139..2768)
                     /gene="upp"
                     /locus_tag="IV038_000199"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP8340451.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(2902..3849)
                     /locus_tag="IV038_000200"
     CDS             complement(2902..3849)
                     /locus_tag="IV038_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8340452.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4002..5246)
                     /locus_tag="IV038_000201"
     CDS             complement(4002..5246)
                     /locus_tag="IV038_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP8340453.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(5421..6446)
                     /locus_tag="IV038_000202"
     CDS             complement(5421..6446)
                     /locus_tag="IV038_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP8340454.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(6468..6647)
                     /locus_tag="IV038_000203"
     CDS             complement(6468..6647)
                     /locus_tag="IV038_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340455.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(6640..7479)
                     /gene="prmC"
                     /locus_tag="IV038_000204"
     CDS             complement(6640..7479)
                     /gene="prmC"
                     /locus_tag="IV038_000204"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP8340456.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(7472..8545)
                     /gene="prfA"
                     /locus_tag="IV038_000205"
     CDS             complement(7472..8545)
                     /gene="prfA"
                     /locus_tag="IV038_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP8340457.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(8548..9132)
                     /locus_tag="IV038_000206"
     CDS             complement(8548..9132)
                     /locus_tag="IV038_000206"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP8340458.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9238..9707)
                     /locus_tag="IV038_000207"
                     /pseudo
     CDS             complement(9238..9707)
                     /locus_tag="IV038_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9813..10415
                     /locus_tag="IV038_000208"
     CDS             9813..10415
                     /locus_tag="IV038_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8340459.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(10519..11088)
                     /gene="lepB"
                     /locus_tag="IV038_000209"
     CDS             complement(10519..11088)
                     /gene="lepB"
                     /locus_tag="IV038_000209"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8340460.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(11318..12085)
                     /locus_tag="IV038_000210"
     CDS             complement(11318..12085)
                     /locus_tag="IV038_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340461.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12082..12561)
                     /locus_tag="IV038_000211"
     CDS             complement(12082..12561)
                     /locus_tag="IV038_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP8340462.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(12686..13423)
                     /locus_tag="IV038_000212"
     CDS             complement(12686..13423)
                     /locus_tag="IV038_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP8340463.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            13584..14984
                     /locus_tag="IV038_000213"
     CDS             13584..14984
                     /locus_tag="IV038_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP8340464.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15207..16559
                     /locus_tag="IV038_000214"
     CDS             15207..16559
                     /locus_tag="IV038_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP8340465.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            16552..17250
                     /locus_tag="IV038_000215"
     CDS             16552..17250
                     /locus_tag="IV038_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP8340466.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(17312..18463)
                     /locus_tag="IV038_000216"
     CDS             complement(17312..18463)
                     /locus_tag="IV038_000216"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP8340467.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(18476..18913)
                     /locus_tag="IV038_000217"
     CDS             complement(18476..18913)
                     /locus_tag="IV038_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8340468.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(18928..20694)
                     /locus_tag="IV038_000218"
     CDS             complement(18928..20694)
                     /locus_tag="IV038_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP8340469.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            20958..21635
                     /locus_tag="IV038_000219"
     CDS             20958..21635
                     /locus_tag="IV038_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340470.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            21638..22765
                     /locus_tag="IV038_000220"
     CDS             21638..22765
                     /locus_tag="IV038_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340471.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(22965..23405)
                     /locus_tag="IV038_000221"
     CDS             complement(22965..23405)
                     /locus_tag="IV038_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8340472.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(23416..25449)
                     /locus_tag="IV038_000222"
     CDS             complement(23416..25449)
                     /locus_tag="IV038_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8340473.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(25451..27154)
                     /locus_tag="IV038_000223"
     CDS             complement(25451..27154)
                     /locus_tag="IV038_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8340474.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27248..28198)
                     /gene="manA"
                     /locus_tag="IV038_000224"
     CDS             complement(27248..28198)
                     /gene="manA"
                     /locus_tag="IV038_000224"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP8340475.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28237..29262)
                     /locus_tag="IV038_000225"
     CDS             complement(28237..29262)
                     /locus_tag="IV038_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8340476.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(29471..30340)
                     /locus_tag="IV038_000226"
     CDS             complement(29471..30340)
                     /locus_tag="IV038_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP8340477.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31044..32936)
                     /locus_tag="IV038_000227"
     CDS             complement(31044..32936)
                     /locus_tag="IV038_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340478.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(32933..34660)
                     /locus_tag="IV038_000228"
     CDS             complement(32933..34660)
                     /locus_tag="IV038_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340479.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(34676..35326)
                     /locus_tag="IV038_000229"
     CDS             complement(34676..35326)
                     /locus_tag="IV038_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8340480.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            35530..35826
                     /locus_tag="IV038_000230"
     CDS             35530..35826
                     /locus_tag="IV038_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8340481.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36018..36638
                     /locus_tag="IV038_000231"
     CDS             36018..36638
                     /locus_tag="IV038_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP8340482.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(36730..37896)
                     /locus_tag="IV038_000232"
     CDS             complement(36730..37896)
                     /locus_tag="IV038_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8340483.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(37908..38261)
                     /locus_tag="IV038_000233"
     CDS             complement(37908..38261)
                     /locus_tag="IV038_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8340484.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            38389..39411
                     /locus_tag="IV038_000234"
     CDS             38389..39411
                     /locus_tag="IV038_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP8340485.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(39850..40284)
                     /locus_tag="IV038_000235"
     CDS             complement(39850..40284)
                     /locus_tag="IV038_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340486.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(40394..42253)
                     /locus_tag="IV038_000236"
     CDS             complement(40394..42253)
                     /locus_tag="IV038_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8340487.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(42371..43531)
                     /locus_tag="IV038_000237"
     CDS             complement(42371..43531)
                     /locus_tag="IV038_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP8340488.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(43838..45262)
                     /gene="araA"
                     /locus_tag="IV038_000238"
     CDS             complement(43838..45262)
                     /gene="araA"
                     /locus_tag="IV038_000238"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP8340489.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45278..45976)
                     /locus_tag="IV038_000239"
     CDS             complement(45278..45976)
                     /locus_tag="IV038_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8340490.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(45933..47594)
                     /locus_tag="IV038_000240"
     CDS             complement(45933..47594)
                     /locus_tag="IV038_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP8340491.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47812..49212
                     /locus_tag="IV038_000241"
     CDS             47812..49212
                     /locus_tag="IV038_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP8340492.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(49464..50543)
                     /locus_tag="IV038_000242"
     CDS             complement(49464..50543)
                     /locus_tag="IV038_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8340493.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(50629..51588)
                     /locus_tag="IV038_000243"
     CDS             complement(50629..51588)
                     /locus_tag="IV038_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP8340494.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(51600..53123)
                     /locus_tag="IV038_000244"
     CDS             complement(51600..53123)
                     /locus_tag="IV038_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8340495.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53133..53696)
                     /locus_tag="IV038_000245"
     CDS             complement(53133..53696)
                     /locus_tag="IV038_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8340496.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(53702..54565)
                     /locus_tag="IV038_000246"
     CDS             complement(53702..54565)
                     /locus_tag="IV038_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8340497.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(54579..55475)
                     /locus_tag="IV038_000247"
     CDS             complement(54579..55475)
                     /locus_tag="IV038_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8340498.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(55650..56921)
                     /locus_tag="IV038_000248"
     CDS             complement(55650..56921)
                     /locus_tag="IV038_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8340499.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(56911..58428)
                     /locus_tag="IV038_000249"
     CDS             complement(56911..58428)
                     /locus_tag="IV038_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP8340500.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            58626..59516
                     /locus_tag="IV038_000250"
     CDS             58626..59516
                     /locus_tag="IV038_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP8340501.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(59589..60413)
                     /gene="nadE"
                     /locus_tag="IV038_000251"
     CDS             complement(59589..60413)
                     /gene="nadE"
                     /locus_tag="IV038_000251"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP8340502.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(60425..61897)
                     /locus_tag="IV038_000252"
     CDS             complement(60425..61897)
                     /locus_tag="IV038_000252"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP8340503.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(62534..62896)
                     /locus_tag="IV038_000253"
     CDS             complement(62534..62896)
                     /locus_tag="IV038_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8340504.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63119..63871
                     /locus_tag="IV038_000254"
     CDS             63119..63871
                     /locus_tag="IV038_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8340505.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64011..64934)
                     /locus_tag="IV038_000255"
     CDS             complement(64011..64934)
                     /locus_tag="IV038_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8340506.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(64949..65221)
                     /locus_tag="IV038_000256"
     CDS             complement(64949..65221)
                     /locus_tag="IV038_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340507.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18900 a        13408 c        11390 g        21695 t
ORIGIN      
        1 agttggttta gcttttgtta atatgacttt atgaaaatgg aggtaattaa atatgatttt
       61 tcctaaagtt aatgaagcaa gcttgaataa aggatccttt tttcatatct atgaaaacga
      121 agattttgaa ctgtataata tcctttcaca aaaagaggtc atcgaaaacc aaataatctg
      181 ccatccaatt atttacaaat ctattttcaa gtatgcattt tttgcttcca atgattttca
      241 aagaatggag attaccgact gtatattcga aaactgtgat ttttcaggct gtatattgac
      301 aaaaagcatg ttgcatcgcg tgaaatttgt caattgtaaa ttaacaggta ctaaattgat
      361 ggaaagttat attgggaaca cgttattcga gaattgtaaa atggactatg taaatttatc
      421 tggttcaaac ataaaagaaa gtacctttga gcactctgtc ttatcaagcg cagattttgt
      481 agactgttcc ctcactaaaa taatgttttc tactaatgat ttagaaaatg ctagcttttt
      541 tgatactgat ttaaaaaata tcgatttaag tagcaactcc tttgaaatga ttgaagtacg
      601 ctcggaacat ctaaaaaatt gtacgcttaa tcagttgcaa gcacttggtt ttgctagaaa
      661 atttttacaa ataaaagtag tataatcaaa atgaatatat aagataaatt tttagcaata
      721 caaatatcca taaaaaaaca ttgcctattt taactcttca tgtatactga ctctatcttc
      781 aaaaactgaa tagtaggagt tattatcatg aataacattt taggaaaaaa aatagaactt
      841 aaaaaaggga tattgggcac tattgtcgtt gtactcggtt tgttagggtt gctatttgcg
      901 ccatccatta ttagcatttt ttctttaact atcactggat ttatagcatt tacacttgtt
      961 tttggtaaaa aagatacgaa aaaaatgttc tcgaaacctg tcgctcctgt caaaaatata
     1021 gccaaatatt tttttatcaa tgtagtaatc agtgccgcag tctcggtatt tttgcaacaa
     1081 attttgaaat ggggtttaac aggcaatcca ataaatgagg ctttcagccc gttattattt
     1141 attattttgc ctatcatgat acttggagaa gaattgttct cagtgtattt tctcgctatt
     1201 ttttctagta agttcagcat tcctgtcgct agtttcttgt cagcaatcat ctttggattt
     1261 atccattaca gtacttatga taatggaaat attttacaca ctgtagcaca catccttttg
     1321 atacaagggg tggcaagatt gttattcaac caagctgcca ttaaaagcaa ctcgatcatt
     1381 acaagttggg cagttcatgt gatatttgat ttcacagcta tattgctagt cgttttattc
     1441 tcctaaattt ttatagaaat aatacatgct ctattgctaa gaaagcatac tttatttcct
     1501 ttaaacaaaa agacagatgt atcaatggat taaaaatcct tgatatatct gtcttctctt
     1561 ttaatgccag ctatagggat cgaacctacg acctacgcgt tacgagtgcg ttgctctacc
     1621 aactgagcta agctggcctt atccaaaaga aattatatac tctatttggc tgattgtaaa
     1681 gagtccgatg gaaaattagt ttaatttcat gatatttttc ataaaacgtg gtataattga
     1741 tgtgaaaggc aggttttatg aaaatggcaa cttttggaaa atttgataca actatcgtac
     1801 catcggaaat tggtgaaaaa ttcaagatca atactcacgt aatgttcaca attcacggca
     1861 agtcgcaaac tggaacgatt tcaaagcaat tgaaaaattc cgctgttgtt gagattgatg
     1921 aaactgcaga aaatgcgaac ttgatgacgc aaagtaaggg tgtagtaatc gttaactaca
     1981 aagaactaaa aaagatttga atttgaccat ctctgcattt ttgggatggt ttttttatat
     2041 gcaaacgaaa gctgtgaaaa agctgttctg catcaaacgt taaaaatgac caaaaaagga
     2101 tgtgacaaaa gttgtgtcac accctttttc gtatttactt attttgtacc aaacaaacgg
     2161 tcacccgcat cccctagtcc aggaacgata taaccatgtt cattcaattt ttcatctaga
     2221 gaagctgtat agatgtcaat atcaggatgc gcttcttgca aagcttttac tccctcaggt
     2281 gctgctacca aacaaacaaa tttaatgttc gacgcgccac gttctttcaa tgcatcgatt
     2341 gccatgattg ctgatccgcc tgttgccaac attggatcta ctacaaacaa ctgacgtgca
     2401 tcaatgtctt ctggaagttt gacgaaatat tcatgtggtt ccaatgtttc ttcatctcga
     2461 taaagaccga tatgtcccac tttagcagct ggaatcaatt ccaagatacc gtcaaccatt
     2521 ccaatacctg cgcgcaagat tggaataatc gctacttttt taccagacaa cgttttttgt
     2581 gttgtttcag tgatcggtgt ttcgatcacg atgtcttcca atggcatatc ccgtgatact
     2641 tcatatgcca tcaacatagc gatttcatcg actacttcac gaaaaacctt tgttccgcag
     2701 tttttgtccc gaataatagt tagtttgtgt tgaatcaatg gatgatcgat cacttgaaat
     2761 ttgcccattt tttaagctct cctttgttct gtcgtttctt tttaattgta aaacaaaatc
     2821 aagtgattga ctagtcaaac tcgccatttt tcctaagaaa atccgttttt gaaaagaagt
     2881 tttcctgcgg acaagcaaag cttattcttt tttcttgatc caagaagtag ataatgccat
     2941 tgctgcacgc gttgcctttg tctgatccat agcatttacc atgacaaaat catggatgat
     3001 tccttggaag cgtacttgtg taacaggaac ccctgcatca cgaagtttgc gcgcataagc
     3061 ctcaccttca tctcttaata catccgcttc tcccgttaaa atcattgctg aaggaagatc
     3121 atttagttca tttatatttg ctctcaaagg cgaagctgtg atttcagcac gctcttcttc
     3181 attcgtcgta tattggtccc aaaaccacat cattccttct cttgtcaaaa aatagttttc
     3241 tccgaattca tgataggaat ctgtatcaaa atcagcgttt gtgactggat aatacagcaa
     3301 ttgcttggat attgggaggc cctttcgttc ttttgttaag atcgtcatga ctgtagccat
     3361 gttaccacct actgaatctc ctgcaacgat tagattctcg agatccagat tttttgactt
     3421 ggctaattta ttcagctgtt gcaacacagc ataattttgt tcaattgctg ttggatattt
     3481 tgcttctggt gatcgactat attctggaaa gacaacgacg gaattcgtac gaaccgcaag
     3541 ttctctgatc aatttatcat gggtttgagc actgccaaaa acccagcctg ctccatgaat
     3601 ataaaaaata actggtaatt tatccatatt cccttctggt cgtacgagac gaatattgag
     3661 ttttccccat tcgccagtat ccaccatcaa gtcttcaata tcgacggaat atttgttcac
     3721 aggtgattct tgtacttctt ctagtaaaac tcttccttct tccggtgcta gttcatagat
     3781 acgcggatga ggcgcatttg catcactgaa tgctactgct tccttttcta acgagatacg
     3841 atcagtcatg acttttcctc cctgagattt agtgtgaaga ctaaaattac acctcatata
     3901 ctagagagca tcaccgatat tgtcaaagaa tccgttttcc caaaaaataa acagaaaagc
     3961 ccaacattga acttttctgt ttactaatgg aatttaaatt tttatttttt atgcaacgga
     4021 aatttttcag ttaattcgcg tacacctgtt ttgacttcgt ctagttgagc gttatcatct
     4081 tttgcttgta atgctttgac aactagttca gccactttta cagcatcttc ttctttgaac
     4141 ccgcgagtag tgattgctgg cgtgccgatt cgaataccgc ttgttttgaa tggacttagt
     4201 gtttcaaaag gaatcgagtt tttatttaca gtgatattca cactgtccaa aatgctctct
     4261 gcttcttttc catttatacc taactcgcga acatcgatca acatcagatg attatctgtc
     4321 gcccctgaaa tgactctggt tccaattgct tgattgaaaa ctttgaccat tgcttttgca
     4381 ttggcgatga tttgttcact atattcttta aatgcaggat ctaatgcttc tttgaatgct
     4441 actgctttac ccgcaatcac atgctctaga gggccgcctt gaataccagg aaataccgca
     4501 ctatttattt tttttgctaa tgcttcatcg tttgtcaaaa tcatgccgcc tcgagggcca
     4561 cgcaatgttt tatgagtggt agtcgttgtg atatctgcat aaggaactgg attaggatga
     4621 agtcctgctg ctactaatcc tgcgatatgt gccatatcta ccatcagttt tgcgcctact
     4681 tcatctgcga tttctctgaa tttcgcaaag tcgatcgttc gtccgtaagc actggcacct
     4741 gctacgatca attttggttg atgttttcta gctaaaatac gtaccacatt ataatcaata
     4801 acttctgttg tcggatcaac accgtaagca acaaagtgat aggttttgcc actgaagttg
     4861 actggtgatc catgtgttag atgtccgcct gcggaaagat ccattcctaa gatcgtgtcc
     4921 cctggttcta ccaaagcgag ataagcagcc gtgttggctt gagagccgga gtgaggctgg
     4981 acgttcgcaa atttcgctcc gaataattct tttgcacggt caatcgctaa attttcaacg
     5041 atatcgacaa attcacaccc accataatag cgatgccctg gataaccttc tgcgtattta
     5101 tttgtcaaga tacttccttg tgcagccatc actgcttcgg agacaaagtt ttcagaggca
     5161 atcagctcca aattatgttc ttgtctttct tcttctttgg cgatcgcagc ccaaagatca
     5221 gggtcaaacg ttttgtaatc taccacaaac aacagctcct ttttattttc ctacaatttt
     5281 atttttccca tcaaaaacaa attgccaaat attcgtatct cttctattac gattagttac
     5341 gcttagtttt tcgttcactg atagaagatt tacttgatag cattgtatca tactaaaaga
     5401 atgcgacaat aaccaattga tcatttttca aaataatttt gtcctgctgc ttttttcaag
     5461 cgattcatgt aagcattccc caatccagtt tctggataca cttgtacaaa aatcaaatct
     5521 aatccaagtg ttggttcatc taatcctctt agacctgcaa acaatccttt agccgccgct
     5581 tctactgagt tgtcgttata catataaaca gctgcagtat ctgtccgcac ttgatccgca
     5641 atttcggggc ttgctatgac acctgcacgg atcttcttat tttttgccca ttgaactgca
     5701 gttgaccaat cgccatctct gaccatcaga acacgtgtat cgggtgagta gtgtttgtat
     5761 ttcatcccag gggcttttgg tgtttcattt tcttttacaa gatgctgatc aatagcaaca
     5821 ggagattcga tgactgcttc gatttgttct ttcgttactg cacctggtcg taaaatcata
     5881 ggcatggctg ttggatcact taaatcaaga acagtggatt ctacaccaat tcgcgtagct
     5941 ccatcatcaa taatcccggt aatttttcct tgtagatcat gatatacatg atctgcagtc
     6001 gttggacttg gttttcccga cgtatttgca cttggaccga caagcggaac accggaaagt
     6061 tcgattactt ctagtgtttt tttgttatcc ggtatacgaa atgcagctgt ggataatcct
     6121 cccgtcacaa ctgagggcaa agtgtctttt ttgatcttaa atatcaaagt taatggtcca
     6181 ggccagaagt tcttcacaat cttctctgct gccggatgaa agttgtccac atactctttt
     6241 acttgttcaa aagaggcgac atggacgatt aatggattat cttgcggtct cccttttaca
     6301 gaaaacactt tcttgacagc gtttggtaaa agagcgttgg cgcccaatcc gtatactgtt
     6361 tcggtaggaa aagcaacaag ttctccttct ttcagtgctt cggctgcctc ttgcaattct
     6421 gtttcatcat atcgtttcgt ttccacagtt tatccacaac ccctttttca tacattctta
     6481 ttttgtctga gtttatcctc aagttttcca ctgtttgtgg ataatctatt tgtcaatagt
     6541 ggataaactt gttgataaac caaaaagtcg tttacatcct tgtcaaataa gtgttttttt
     6601 ataataaaaa aacaaataaa aaatttttca tccacaactt caaccataaa cttggatcat
     6661 tcgttcattg ccaaaaagat ccttttttat cgccactttt ttatctggaa atgcttgctg
     6721 aaatattttt tttactgctt caccttggcg aaatccgatt tctaaaaaaa tctttccatt
     6781 tgattttagt acgcttggtg cttcaactgc tattttttca taaatagcta atccatcgtt
     6841 ctcagcaaaa agggccattt taggttcgaa tgaccggaca ctttcatcca tcagttccca
     6901 ttcattccga ctgatgtacg gcggattaga aataatcaca tcaaattgct gatccattac
     6961 tggtcctaat gtatctccat gataaaaact gattttcgtt ccttctttct gagcattttc
     7021 tttcgctact tctaatgctt cttctgaaag atccacagca gatatatgcc aattttttct
     7081 tgctgctttc aaactaatag caattgctcc agtccctgtc ccaatatcta tcacttctaa
     7141 cggtacacca ggcgtctcgt ctaaacacca ttcgactaac tcctctgttt ctggtcgagg
     7201 gatcagtgtt gcttcagtta cctttaaccg atgatcataa aaatctgcat atcctagcaa
     7261 atactgaggt ggatgatctg caagcagaag tcgcagatcg gtttcgatca accgctgttc
     7321 ttcttctgaa atctcttcgt tcatatgaag gagccaatca agttttttcc attgttttcg
     7381 ttccaaaaat aaaaattgga tactatatgc ttctttccca tgatcctcta aaaaagaaga
     7441 agcccgtgac aggacttctc gataggtcat tttacccatt ttgcatagct tccagttttg
     7501 atgtttgatc atacatgatc aatgcatcaa cgatctcatc taatttccct gctaaaatct
     7561 gatctaattt ttggatagtt aatccgatac gatgatcagt cacacgattt tgcgggaaat
     7621 tatacgtccg gatccgttct gaacgatccc cagttccgac tgctgatttt cgattggcat
     7681 catactcgct ttgtgcttct tgctggatct tatcgtacac acgagcacgc aagattttca
     7741 ttgctttttc acggttcttg atttgagaac gttcatcttg catagcaaca acgatccctg
     7801 ttggtaagtg agtcaaacgt acagcagatg ctgttttatt gacgtgctgt ccgccagcac
     7861 ctgaagcatg atagatatct gtacggatat ctttgtcttc aatatcgatc tctacttctt
     7921 cagcttctgg catcacgaca actgttgcgg tggaagtatg gatacgccct tgcgattcag
     7981 ttgaaggaac acgctggacg cggtgcgcac cactttcata ttttaatttc gaatatacgt
     8041 tgtctccggt gatcatcatg atcacttctt tatatccacc gattccggta acgttagctt
     8101 ccatcacttc tgttttccag ccttgtgctt cagcatactt ttggtacata ttgaaaagat
     8161 cgccagcgaa taatgctgct tcgtctccgc ctgctgctcc acggatttcc atgatgatat
     8221 ttttatcgtc atttggatct tttggtaaaa gcaagatctt gattttttct tctaagactt
     8281 ctttttcttt ttttaattca gataattctt cttttgctag ttcagccatt tcatcatcca
     8341 gtttttcacc aagcagttct tctgtatcgg tgattccttg tactacttct ttataacgac
     8401 gataaacttc gactgtttcg cgcgtgtttg cttcttcttt tgaaagctgc ataaaacgct
     8461 ttgtatcaga aatcacttct gggtcactta aaagttctcc aagctcttca taacggtctt
     8521 caattgcttg taattgatcg tacatgctta ttctccttct gtaatcattg atgggtgaaa
     8581 gtaatgtttt cgacaaacgg ggtaataagc ttcatttccg ccaatttgca cttgatcgcc
     8641 ttcataaaca gggtttccat caatataatg taaattcatg attgcttttt tgtggcagaa
     8701 ccaacagatt gttttcattt cttcgatttt gtctgcataa agcaataaat attttgatcc
     8761 ttcaaataac tcgtttcgaa aatcattttt caaaccaaat gccataaccg gaatatccaa
     8821 ttcgtccaca attctcgtaa aagcaatgac atgtttcttt tttaagaatt gcgcttcatc
     8881 gatcagcaca caatagggtt tgctttccat tttctgaatt acgtcaaata cgtctgtttc
     8941 ttcaaatatc ggcatcgcat tacgtttcag tccgattcgg cttgaaacaa cgcctaaacc
     9001 atctcttgta tcaataccgc ttgtcattaa gacaactggc ttattttgtt cttcataatt
     9061 atgtgcgact tttaggatct cgatcgtctt gccgctattc attgcgccat acttaaaaaa
     9121 tagttgtgcc atgctctttt tcaccttcca gtttgttcct atggtattat acttgatttt
     9181 cagcattttt tacagtgaga attttcatca agcacagatt agtattaaat ctattgctca
     9241 cttttgttcc aattttttga caagaactaa acatgggttc cattcatccc ataaatttgg
     9301 aaaaacttcc aaccttgaaa atcctaatga ttcgtagaaa caaatagttt gatcatatat
     9361 agagtagtgc ccctcgtcta cagtcttcac ttgcaaatat ttgtaatgtt ttgctgcgta
     9421 accttctgct tctttaaaaa gttttgttcc taatccttgg tgatgatagt ctttttttat
     9481 tcccatacaa tagaatagat ttcggcagta tcttcacttg tctgtgaaag agtgataaag
     9541 cctaaatatt cttcttttct tttttcacac cataaaggta gtttacttga ttcctcgata
     9601 tatttttccg ttgattcagg aagcccaaac cactcaggca agtctagtaa tatttctcgg
     9661 acacactttc ctttttcctc tctatctttt acaaacgata tcggcatatt ctttctagat
     9721 tctccctttt tgaattgagt atagcatgct ttctttcttt tcacgtgcta taataagtta
     9781 aaatttcaaa gttttacgta aggagctatt tgatgacaaa aaatattttt gattccatcg
     9841 ctaaccatta tgattcccct gaccgtcaag cattagccaa gattattcgc gaagaactaa
     9901 ctacttatct accgcgcgac agccaccaaa aagtcctgct ggattacgga ggcggaactg
     9961 ggcttgttag tttaccgctt gcggaaagat tcaaagaact gatcattgcc gatgcttcag
    10021 aaactatgct gaaaatggca gaagaaaaga tccaagcagc agatctgaaa aatgtccgaa
    10081 cgatccacgc tgatgcttct gtcgaatttc ctgctgtcca agctaatctc atccttctat
    10141 cacttgtact tttacacatt cctgatacgg agaatattct tacaaaactt tatgaaattt
    10201 tagctcctgg cggtcaattg atcatcgtag actttgacaa gaatgaacaa attagtcatc
    10261 cgaaagtcca taatggcttt agccaagaag aattgaataa tcgattggaa aaaacgggct
    10321 ttgtctctgc tactagtcat accttccatc gtggggaaaa gctatttatg aacaaaaacg
    10381 cttcgctttt cttatccatc agccaaaaag cttaaaatga gattgcaaaa aaagtgagca
    10441 actccaagaa ataagaagga attttcggaa aaagaagctg tgacaaaaaa catgtcacag
    10501 cttcttttta ttttttcatc acatgatttt aatttctgaa agaggccaat aacggaaaac
    10561 aacttttcct tggatcatat ctttgtcgac taacccaaac attcggctgt cttttgaaat
    10621 caagcggtta tcgcctagaa caagatactt tccttttggc acagtatatt caaagtcact
    10681 cgtaaaggtg gatgaacttt ctgcttgtgc ttggaatcct gagctataag catattctcc
    10741 ttgtaactta tcttctgaaa attctttttt gaactcatcc agataaggtt catcatactt
    10801 tttgccatta atggtcaact ggtcattttc catcttgact gtatctccag gcataccgat
    10861 gatccgttta acaatcatcc gttcttcatc ccccggttcc tttgtagtaa taatatccat
    10921 tcgttcatag tttgagatct tagatgaaat caatcgttgt ccatcactta atgtagggtc
    10981 catcgaatgc ccgtttacag taactggtgt aaacacgtac actctagcta ataaaatagc
    11041 cgctaataca aggatcaaaa tcccccaatt tttcaaaaat tctttcacag cttacctcct
    11101 acttgtattt tcctcgttat ttattacgtt ttttcacaat acttcgttgt actttcttta
    11161 ttctacaatg tttataaatg aaaggaaaga ataagtgatg tgttcgtctt atctttctcg
    11221 ttatataaaa aaggaacgtg acaaaagacg tgtcacatcc cccttattcc gaataaacgt
    11281 ttttatcgca accactgata cacggcgttt catcacatta tgaatgtctt cttctttggc
    11341 tacgactata aaccactggt tcttcctgcg actgtgcctt ttttgcaatc aatttcagac
    11401 gataaggctc atgatagcgt aacccgtctt tgacaaaatc accgccgacg atgaacaatc
    11461 cgacaggttc caatgtgtct tctgttgagt caagtcgaat ggtccctact ttttccatgt
    11521 ccgagtagct tcgtccaata tttagaacat actctttctc tcctgttttc ttgacatagt
    11581 aataaggata caatgtattt ctatcgacca gaaatccatc catgcgaccg agttcatttg
    11641 ttttgatctc accatttcca atcaaatacc gcatattctc ggctaatttc aattgataat
    11701 cttcgtcata agaaaccatg acctcttgtg tattcacttt atcgatcgaa tttccattat
    11761 ctatcgattc ttctgtctgg atgttgaacg cttcatcagg taaatacttc acaaaaagct
    11821 gagtgacatc ggctttcgtg tttttatcta aaaagtaata aaatacattt aaaacagtaa
    11881 aataaacaag cactagaaat gcaagtccat ataagaaaaa ttgaagatag ttttggtttt
    11941 gccaaagtcc ataaacaatt ctcaataaat aaagcgtggg aatcacactt aaaatcgtat
    12001 atccacggtt aagatattta ggactgatgt ctaaatagcc aaggaattta tgcaccccgt
    12061 tgataaggtc tagtaaaaat gtcattgaga atcacctgct tgctggttat acgtttgggt
    12121 atttcctgaa tcttggtttt gctgtgctgt acgtgctgca tcatcatttt gcgtgttatc
    12181 atctacagtc gtactgcttg gtatcgtttc ggagctagca cttgtatccg gacttgtact
    12241 tggattggtt gaatccgatg gagtgccatc tggtaccgta ttacttgttt cgcttgagct
    12301 gtcagttgta tatgtctcat catacgacga actagttgtt gtatcgtccc atgtacttgt
    12361 cgtagtggtt tcattcaaac tttccgttgt tgttggttca gatgtcgaga ctgaagtagt
    12421 tggtgttgtt tcttctacag tactagaact tgaacttggt gcacggtagc tggaactatt
    12481 cacaatacct gtagtagtcg cgacaatcgt cgagattgtt aatacagcga ttggcttcaa
    12541 aataggtgct acttttcgca tcttatctct tccttcattg aaatttattc ttgtttcctt
    12601 actttactac ggacaaatta tacagtctac cttatttcta ccataaattc tttatttttt
    12661 tcttacgttt ttccgaaaga tttctttaat tttcctcaaa aactacggtt gatgttttta
    12721 agtaagcttt tgcttcccga ttgatttctt tcagatcgca tccttgctgc ttggcagcga
    12781 acacgcaacc acaataacat tggcgataaa tatcatactc tttgcacatt tgcaaagagc
    12841 gttcatagcc tttgtttttc ttgaaatcac ttggcaagta ttgtgtcccg taaatctttt
    12901 ggatatccat tccgatctga ttgatcaaag ctgcattttt ctttggcgag atggtcaggg
    12961 cacttccaaa gtaatcgtat cctagttctt gcgctttttg cgcaacaaga tctaatcgca
    13021 tattgaagca tgcactgcag cgagcaccac cttctttttc gttttcaaga tggtttgtta
    13081 aaaccatttt ggtgaattca tttggacggt attcatcaac gattagcgca acatgatggt
    13141 cagttttctc attaaattgg tctacaaatc gtttttgttc atgtaatctt cgcaaatact
    13201 cacttttagg atgaatattc gaatttgaaa aaaataacgt gatctctgca tattgagaca
    13261 tgaattccaa tgtgtgcgta ctgcaaggag cacagcatgt atgtattaat atcttaggct
    13321 ttacttcttc tttctcccat tgcatgatca tttttttcag aactgtatca taatttattt
    13381 tttgattttt cattttttct acgatttgtt gagcattaat caatttgtta ttcctcctcc
    13441 ttcttttttc taggattttc cttattataa gccatttcta ttttaatcga aagctgtatt
    13501 gttatctgtt tgataatttc tttgagtatt cctaattccc cttgtatact ggtagaaacg
    13561 actattgtca ggaggatagt actgtgcatg ttgatttatt ctttcgaagg ctgcagcggc
    13621 gaggacagaa gtttgctgcc aatcatttct catcgatcca aatcatcgtt ttttattata
    13681 tattgatgac ggtcttatca ctcgttctat tttatatgcc gatttttcgc gaaccagata
    13741 gccatgtttc ttttgtggac atgctgttca tggcaatcag tacagtgagt gtgactggac
    13801 tatcgacttt tgatatcaat tctgtgttca atgataacgg tattatactg ctggaaatat
    13861 tatttcaagt tggtggtttg gggatcatga tgatttctac tgcttttgtc attttttcta
    13921 aacgacggat cacactgaaa cagcgacagc tgatcatgac ggatatgaat caaccgcgtt
    13981 tgtctggtat cgtccgactg attcggatca cttttgctat tttaatctgg tttcaattac
    14041 tttttgggac attcttttct atttattttt attacagagg ctattttgat cgttggcgtg
    14101 atgccgtttt ttacggtttt tatcaggcga tatcggctgt cacgaactct gggtttgacg
    14161 tcactgggga ttccatcaag ccctttgcac atgactattt tttcttgatc ctgatcatgt
    14221 ttttgatttt tgtcggagga attggttttc ctgtattgat ggagtgccgg gaatggctat
    14281 tatacaaacg ttccaatgcg aagcttccgt ttcgtttttc cttgtttacc aaattagctg
    14341 tgttagcttt tgttatcttg tttgtttcgg gaactgtctt gatttatcta ttggaaaagg
    14401 accatttatt ccaagattcc aatatgagtg tcaaatggat caattcgatg ttttattcga
    14461 ttacgactcg taatgctggt ctgcagattc acgatttggg tgattttcaa atcacaacac
    14521 tgatcatttt ttctttactg atgtttatcg gatgcagtcc tagttctgta ggcggcggga
    14581 ttcgaacaac tactgtagca atcatcggtt tgtatctata ttccttcctc aaaagtgaag
    14641 ataacatcaa tatttttgga cggagaatcg atcaagatga tgtgcgaaaa tcagtcgttg
    14701 tctttatgct ttctcttggt atgtgtttct tttgtatcgt ttttttatct gctacagaag
    14761 aacaaacgct gatctcgatc atcatcgaag taacttctgc gtttggaaca actggtttat
    14821 ctttagggat taccggagac ttgtctgttg tgggcaaaat cacgattgct acactgatgt
    14881 ttattgggcg gatcggaatg ctatatacat tgatgttatt cgtgccaaaa gaaacacgag
    14941 atctaggcta tgaatatcca agcgagaaaa ttattatcgg ttaaaatggg aatataggta
    15001 agagggaata ataaaatcac agcagcttga aacaatcatt ttgtttcaag ctgttttttt
    15061 gttaaatcat gcgattttgt ccgatttcat gcactttcag ccgtaacctt ttcttaagaa
    15121 taagaaaagc aatttaacct ttttttcaac atatggtatg attagttgaa tttattcgtc
    15181 agcaaatcgt ttttggaggt tacataatgg gaatacgcag tcattttgcg attgcagttg
    15241 gaaaaacgtc gcaatgggca ttaaaaactt tttttaaagg cggatcaagt ttaccaggta
    15301 aactagcgct gaaagtcgat ccacatatac tagatagttt ggcaaaagac taccaagtcg
    15361 tcgttgtcac cggaacaaac ggtaaaacat tgactaccgc tttaacagtc aatattttgc
    15421 gccagcaatt cgatgaagtt ctaaccaacc ctactggtgc gaatatggta cagggaattg
    15481 tatcgacttt cttgcaggca aaaccaaaaa aaggacagaa aaagtttgct gtcctagaaa
    15541 tagatgaagc aagtttgagc aaagtcacag aatatatgaa accagaactg ttcttattta
    15601 caaatatttt ccgtgatcaa atggatcggt atggagaaat ctatacgacc tataaaatga
    15661 ttgtagacgg tgccgcaaag tcaccaaatg caacgattat cagtaacggt gattcaccaa
    15721 tcttcaattc tatcgaaaca gtcaatccta gaaaatatta tggctttgac catgaagaag
    15781 atcgtgaaca aatggctcat tacaatacag acggtgtctt atgtccaaat tgtcatcaca
    15841 tcttgcatta taaaatgatc acttatgcaa acttagggaa atattactgt ccaaattgtg
    15901 acttcaaacg tcctgaattg gattatcgat taactgatat ggtacggatg gacaatgtgt
    15961 cagcagattt cgtgatcgat ggtcatgaat acggaatcga agttggcggt atgtataatg
    16021 tctacaatgc tttagcagct actgcagtgg cagagtactt caatgtcgat cctgataaaa
    16081 tcaaacaagg tttaggatat gacgagaaag tattcggccg acaagaagtc atcaatatca
    16141 atgggaaaaa atgtacatta gtacttgtca aaaatcctgt aggtatcaat caagtaatcg
    16201 atatgattgg tctagctcct tatccatttt ctttagtggc tttattaaat gccaattacg
    16261 cagacggaat cgatgtcagc tggatttggg atggtaattt tgaaaacttt gccgatatgg
    16321 atatcccagc tgttatttct ggaggagacc gtcatacaga tatgacttta cgtctaaaag
    16381 ttgccgggat tccagaaaat aaattgacag aaacaaaaga attaccagaa gtaatcgaaa
    16441 aaatcaaaga attgccaact gagcatgtct atattttagc tacttacact gctgtattgc
    16501 agcttcgtaa agaactagct gctcaaggat ttatcaaagg aggaatgaac cgtggctaat
    16561 tatgaactca atatcgccca cctttatgga aatttgatga acacatatgg tgataacgga
    16621 aaccttttga tgttgaaata tatagcagaa aaaatggggg tttcttgcca tacggagatc
    16681 gttagtatac atgaaccttt tgatgctgat aaatatgact tggttttctt tggcggtggt
    16741 caagactttg agcaattaat catttcaaaa gacatccaga aaaagaaaga ttctttgatc
    16801 aactatatcg aaaatgatgg cgtcatgtta gccatttgtg gtggctacca attgctaggt
    16861 cactactaca tgggagctaa tggagaaaag atccaaggaa tcagtgcact ggatcactac
    16921 acgttaagtc aggaaaataa ccggtttatc ggtgatatcg ttatccataa cgaagaattc
    16981 aatgaaactt attatggatt tgaaaaccac aatggtcgga ctttcttagg tgaaggtgaa
    17041 cgacctttgg ggaaagtggt acaaggtcaa ggaaataatg gtgaagacca atctgaaggt
    17101 gtcatttacc gaaatgtctt cggttcttat ttccatggac ctatccttgc aagaaatgag
    17161 aacctggcag ctcgtttgat ccgcctagct ttagaacaac gttacggtaa agaattggat
    17221 ctgccctatc ttgaaggaca aacagcctag ttattcgaaa aaggggaatg tgacaaaaat
    17281 ttcgtcacat tccccttttt cgtatcctat tttaagcgac ttgcttggca taacggttca
    17341 cgttttgctt gatattcccg attgttttcg gatcattgat tcctgtgact tcttgaatca
    17401 attgttccaa gttttcatgg atcttcattt catgcaattg acggctttgt tcatcttcag
    17461 gatcatagta attaaaaata atccccattg ttgccgtcaa atgtgggcaa gtcaagttat
    17521 gttcttgtag ttctctcact ggacgtgtaa atctttcttg ataacctaac ttacgtaatg
    17581 gtgtccgagc gacacgtgtg atcgcatcag agatattttt gttttggaag cgatgaatga
    17641 ttttttcaat atacgcatga tgctgctctg catcaaaccc ccatttggca atcagcaaac
    17701 ttcctgtttc ctgcaaaact gattttaatt gtgccacaac taacgcatct tgcattgctt
    17761 catcaatggt ctggtaacct aacaaggcac ctgtataagc gacagtcgca tgaccagtat
    17821 tgacactaaa tagtttgcgt tcgatatatg gttctagatc atctacatat aaaacccctt
    17881 ttaattggat ttttttattt tttctttggc tatcgtcaat gacccattct ttgaaaggtt
    17941 ccacttggac gaataaagga tcttcatggt gttgtaatgg gacgattcga tcgactgctg
    18001 catttggaaa gccgatatat ttgtcagtaa atgatgtttc cggaagatat ttatacactt
    18061 ctttttctag aaatgctgaa ccgccaatca tattttcaca ggcaatgata tctaaagatg
    18121 ttgtgcttcc ttccttttct ctttcttgga taccttttgc aatcaactca gcaataaaag
    18181 gtaagatatt cgggccaatg gcggtagtaa ctaaatctgc ttcagcgatt tcttggatta
    18241 ctttttctgg atttgtttga ttgttgattc ctttgacatt ttttacagtg attttttctt
    18301 ttgattcatc tgctaattcg atttcgtatt cttttctttc atttaaagca tcaatgatcg
    18361 tgtgattcac atctacgaaa gtaatctcaa atccattagc tgccaaaacc tctccaataa
    18421 atcctcgacc gatatttcct gcaccaaaat gtactgctct catttttctc tctccttaag
    18481 caattgctag attttccgtt acttcttctt tgctgagcgc gtctgctagc tggacaacat
    18541 tgtccatatc actgcaataa agtgcgattt gctgtacaag ttgcaaatgc tcatctccga
    18601 ctcctgcaat accgaacaaa acagtcgcaa tcttttcttc ttcttcggta ccaaaatcta
    18661 caccatctgg tacttgaacg acacagatgc cggatttttt cacatatttt tttgcttcat
    18721 ctgttccatg aggaatcgcg ataaaattcc ccatataaac agataacatt ttgtctctat
    18781 ctaccatggc atcgatatat tcctcatcaa cgcaccctgc ttctacaagc atctgaccgc
    18841 aatagcggat cgcttcttct ttggttttaa aagatcgatt gagctcgatc atgtcgaatg
    18901 gcaaactttg catgctgttt tcctccctta agctgtctgt ttcaaatatt gtgcaatcga
    18961 ttcgtatttt tgcggattga ccatgctgtt gactgataaa tgaatggatt gaggtgcagc
    19021 catctgtact ttttccgcta aactttcttt ggctacgatc agtgaatcag gttcatcttt
    19081 taaatcttgg atattgactt ttcttaacgg gatagttaaa tggttcttgt ctaatatatc
    19141 tttgagcacg gtcatcccca ttgttgctga tccggaacga tcatcgtgga caagtagaat
    19201 ctctttgatt tgtttgatgt ttgctgaaag aaactcttct gctggctcat tctcaatttc
    19261 agaaactgat gttttctgct tgctgcttaa ttcttgaatg atttcatcat atcgaggtga
    19321 attcaaaaag ttttctactg agacaaaacg cgcgctcggt gctttttgtt ttgctctttc
    19381 ttgtagttcg acctgagtaa cgatcaatgt attcgagtca tctgtcagtt ggctgattgc
    19441 ttgattggtc accgtttgag gcaagtctgc ttcctgtact ttttttctca agatcgaagc
    19501 gcccatcgca cttgatccca ttcctgcatc acaagcaaaa atgatttttt gtacgccaga
    19561 gaatgtagct ccttgaggaa taccttgggc acctttggaa gcttgcttag ctgcctttgt
    19621 ctcttgaaca gatttttcga agtcttcatt tgtatctttc gcatccgctt tcaaaattac
    19681 tgcagaaata ccaaacgaaa cagctgcggc aacaatcacg cccaaaataa caggaaggta
    19741 agaattgaac ggtgtcatcg ctagaatcgc aatgatcgac cctggagaag ctggcgctct
    19801 tagtccagca tccatcaatt ggaaaatgaa acttcctgac attcctccgc ctataactga
    19861 taagaataat aacggtttca tcataacgta aggaaaatag atttcatgga tcccgcctaa
    19921 aaagtgaatg atcatggctc ctggtgctga agattttgct gctcctttgc caaacaaagt
    19981 aaaagcaagt aatacgccaa gtcctggacc aggatttgct tcaagcaaga atagaataga
    20041 ttttccagcc tctgctactt gttcagttcc caatggtgtc aaaatcccat gattgattgc
    20101 attattcaaa aataagattt ttgctggttc aataaaaata cttgtcaatg gaatcaaatg
    20161 ggcagataaa atgatctcta caccttttgc cattgcttga gtcaatgcat cgacaactgg
    20221 accaatcgca gaaaaaccta ataacgctaa tccaaaaccg ataagacctg ctgaaaaatt
    20281 attgactagc atttcaaaac cggatttgat ccgtttttgg aatagctgat caaatttctt
    20341 gatggcatat ccaccaagag gtcccatgat catcgcgcct agcatcattg gaatatctgt
    20401 tccgacaatc acacccatcg ttgcaattgc cccaacaact gatccgcggt caccagcaat
    20461 tacttttccg ccagtataac cgatcaataa aggaatcaga taagttagca ttgggcttac
    20521 catggtagcg aaagcctcat ttggtaaata accatctggg ataaatagag ccgttaatac
    20581 accccaggca atcagtgctc caatatttgg catgaccatg cttgataaag tactgcctaa
    20641 tttttgtacc ctcgctttta gcgaaatgtt gtttttggga atcgtccgtt ccattttgtc
    20701 tctccctctc tccttttgat actctaattg tatccgctta ctaaaaagag aaatagtcgt
    20761 actttggcac gaaactttgt gccaaaggtt tgagtatgtc aacaaaataa aaagatttcg
    20821 gtatcagaaa acttcaattt taaataaaac tgttatttta atttttttat aatatgcaaa
    20881 caataacttt tgttagcatt taatttagat gttttccatt ctattatgtt attaaatttt
    20941 atattggagg cctatctatg acattactga tcaaaaacat gaacatgaaa cgtggtaaaa
    21001 aacgtgtgat tcgacaattg aatcttttag tagaatcagg agaagtagct gctttgattg
    21061 ccccgaacgg atttggcaaa acaactttgc tgaatggcat tgcccaactg ctgccaacaa
    21121 cttatgatca attgtccctt cacaacatct ctcctactgc ttatacagat tatcgccggt
    21181 ctttcttttt tctagaatca acgaatcagt tatccgacta tctatcggct gttgattatt
    21241 tgactttgat caaatactat tggcagagca aagtagaatg ccaaactgtt ttccaacgat
    21301 tgaagatgac tgagtacctt actttgccga ttggaaaaat gtctctagga atgaaacaac
    21361 atgtcttgct tgctgcttac ttgatcagtg attcatctgt catgttattc gatgaaccgt
    21421 tgaacggatt agacccagga agtattgata tcttgaatag cctcatcaga caatggcaga
    21481 aagaggggaa gacgattctt ctttcttccc acgctatcgc gaatatccaa tcgatttgtt
    21541 ctaaagcatg ttttctaaaa aatggagaaa tcgtcacaga atcctcaaat atggaagaga
    21601 ttttagctat ttatcgtaaa ttatatgagg tgtaactatg tctttactta catttgaacg
    21661 caatctctta ctaaaaaaca aagtaaactt tttatttcct ctattgttgg tcgtgctctt
    21721 tgcattccca cttttttttg atcataaact tgcttataca gaattcgatg aactgaatca
    21781 taactatgaa gagatgcaac gtttgattga aacgttgaaa gaagatgaga atgagaaaga
    21841 atttgtggaa agtttagaga aaagcaacaa actgatcgaa gcaatcttac atgcaaaaaa
    21901 cacagggaat gtccagcaaa tggtcgaagc aacttatcat tatgaaaaag atatactgga
    21961 tcgactgatt tctggtcaaa gacaaggaat tccaatcatt gaacaacaaa aacgtgtcga
    22021 gttgctccgt tatatgaaag aacatcagat ccaaagatat tctatttttg acttgccagc
    22081 gcatctctct ttagcaaatt actacgaaaa tattttttca ggaatgattt ctagtttttt
    22141 gattttatgt ataaccgcct tgtttctttc ttccatcatc tcttatgaaa aaagaaagca
    22201 ggtaatctct ctagtcaatc tattaccgga tagtatggta aagaaacata gcatccgttt
    22261 taccatttat tatggtgcag ctatgctaag tctcgtgatg ccatttttga tcgtctctat
    22321 actagtaata atcaaaaatg gtttgggaga cttccgttat cctgtaggaa cgatcattgg
    22381 tcaggagata cgaatcctgc cgatgtatga gtatcttttc cagagctttc tgttcctgtt
    22441 gttgtgggtg ctctttttaa gtacaatcag ttttcttcta tctgctttat ttgaacattc
    22501 tctagtcaat cttttaggta cacttctttg tcttttctta gcagagtatc gcttgttttc
    22561 ttcgattgga tggatagaat caatcagtca ttacttgcca acttcttatg ttgattttca
    22621 aaatgtgatc ataggaggag atattttctc gccactagct tcagaacagg ttactttcat
    22681 gaatggtatc ttgacacttg gtatatggag tatcgtttta ttattcatag gtatgggcac
    22741 tatctatata aaaaagagct attaaaacac aaaaataata ggggcggtga caaaaacctt
    22801 gtcaccgtcc cctatttcaa ataaacggtt ttctagaata aatagttttc aaaagcttgc
    22861 atccgcagta ataagaacct cccaacaaaa atagctcctc ttatttttat cttggttgaa
    22921 gttattactt gatgcaaggc agcttttttt actgtgaaca ttggttatat atgtccatgt
    22981 tcaaaaatca tttggacttc ttcaatggtt tccgcatcac tgagcgccat tacttggtcg
    23041 atgtctgaac agaaaaaggc taaatcttgg agcaccgcta actgatgttc tttttttaaa
    23101 gccactacaa ataagagtgt cgcgattttt ggttcctctt ttgttccaaa gtccacacca
    23161 tcaggcactt ggactaaata gattccttct tctactactt cttcatcttt gtcagtccca
    23221 tgtggcaagg ctacgaaatt cccgatatag acactcgctt tttcttgtct ttcgatcatt
    23281 gattggatat atgccggttt gattccccct tgttgggtca tccactggcc gatctcggtc
    23341 aacgcttctt tccaattttg ataggattga tcgagcaata ttttttctgc tggtagctgt
    23401 atcatctttt caccttcatt tctttatttc ttcaatcagg attttcgcta acaactgata
    23461 aacgatttct ttatttcctg agcggaagat ctcggtgttc aagtcattca tgatgattgc
    23521 tccactgatc tttcctaaaa gaatcccgtc tttttcttgg attggcgaag gcgccagcat
    23581 aactaacata cgggacaaat cgactggctg tctatccatc ccttcaatgg tcaacggatg
    23641 tttcaaattg aaaatacaaa agatcggttt agaaatcaat gagctggatg tatggaataa
    23701 ccccatattt gtatgaggaa taccgatagg tgcttgttca tatcgcttaa gcagccggcg
    23761 atgaaccacc tcttcatccg ttactagtcc ttcaggaaaa taaggaaata agctgcgaat
    23821 cgtttcggaa atcgtctcag gattatccag ttcttggata aaaaaacgac cgagtaattg
    23881 attgatttga tccatgaaat gcattgtttc ctcataagta tcttctatca tccactcgtt
    23941 attgctgtta tacgtaattt tttgcctttt tttcttctca atatgcgaaa aagcttcttt
    24001 tagctgcttg acttcatctt ctagcagtag cggggagact aaaatatatt ttccaccgta
    24061 tcctggtaaa atagatgtag aaataacaac atcatattct tcttgtaaat cgattttttt
    24121 taaatcagca atccgataga aatgaatttg ttggatgaag ggaaaatacc ttttcaatcg
    24181 tagttccaac atgctagtag aagctaatcc acttggcgaa aatcctgcaa tactgatatt
    24241 gctttctttc ggacttcgtt ccaatgaatt cgcaaaatga aggaccatat aagcgatctc
    24301 ttcttcggat aatttttttt cttgaaaaat ccgctctact gctttacgga ttgcattcgc
    24361 aacttcctcg tattgaacca tgattttttc taaaatcgga ttggtcagtg tctcttcttg
    24421 taaaacggct cttgaaaaca caccagataa atgagtcaac agcattttgt acaaagtttc
    24481 atcttcataa aaatggacat tcgtctcttc acttacttct tgaatcagtt gttttactaa
    24541 ataagcaagc tgtgtatcga acgtctcttc aaagaaattc tgatcaaatg agttcgtgaa
    24601 gtcatttaat aatcctgcaa aaaagacgat ctcatttact ggataaagtt gttttgtctc
    24661 agaagcgatc cgtgaaaaca atcgtttagc tgtctgtagt aattctttgt ttgccagact
    24721 attatatgtc tcatttgtca gtaaatgatt tttcgtcaca cgctccatcg tcaatgtcag
    24781 catcaaaacc aaatgttcaa gctttctatc acttagattg gtcttttttt ggatcaattc
    24841 ttccccgatt actttgcgtg caagagatag atgttgcgga tcaacgaatc gaaaaaaata
    24901 attcagtgat gcatcgaaag aaatactatg aaaaagttca tattcattga tttctaattc
    24961 taatacattc gctgtcagaa gccggcggta tttttccggt ccttcgatct cgtatccctg
    25021 gttccgaatg atttccaaag gcagttggcg aatactttct tccaactgtt tgatatctgc
    25081 ataaaaagtg gtattactga tgcgatatga ttccatgaaa tgagcaagac tcaacggctc
    25141 tgtcgtcagt aacaactcgg ctaaaatcgc gtgttgtcgt ttagcagtgg ataagtcatt
    25201 acttttccca tctgtatttt cttttaattg ctctaatact tcttctgatg cctcaatgac
    25261 aaatgagcct ctagctgctt ttttcaaaga agcatttacc gatttcaatg agacttcaag
    25321 attatctaat tcgcggtaaa tagtccgttt actgaccttc aataaattca tcattttttg
    25381 gatagataca ggtgacgggc tactgagaaa ttctgctaat aattgttttt ctctgggcga
    25441 taaatacatc ttatatttct cccttcacga gtttttcgta ttcattcgta gctacccaat
    25501 gttcaactag aactagccga gggatctttt cttctagctg ttcagttaca gctagttctc
    25561 tttgtgcgat ataaatcgtt tcttcttgga ttttgatttt ttctattgat tcttttgaga
    25621 taacaaactg ttttttcgct ttttttgcta attgacttaa aatcgtcatg cccatagttt
    25681 gtgagccgcg tctgttgttt ttatacaaaa atacaatatt agcaggcgac gttttttgtt
    25741 ctgtgatatc tgctatctct tttttttctt cccgctttgg aaattggatc gcttctttta
    25801 ttcgtgacag caagatatct gtttccaaaa agtgatccag tacatagatt tgactttgag
    25861 gtactttttc tcttgcaaga cccgctagtt catgttgcac gataaccagc tgatttactt
    25921 ggttattcaa ttcatagata gagctgtatc cgacagaaag atcgattttt tctgctgcta
    25981 atttcttttt cagcagggcg gctcccatag cacttgatcc catacctgag tcgcaagcaa
    26041 aaatgacgga atgaatgata gatggttcca agatttctcc cctttctttg accgttttgt
    26101 tctctttttc tttatttggt aatttcaata gtatcgctgc acaaatgaag gtcacaaatg
    26161 tactgattgc tatccctata cttaccccag ctagttgtgg ataaggcgca ttggcaaaaa
    26221 tcacgagtag tgacccaggc gatacaggtg cagctaatcc cgtatctaac cattgaaata
    26281 tcaacgtgcc gcttgcccca ccagccatta cagctaaaaa tagatacggt cgcagcaaga
    26341 caaatggaaa atagatttcg tgaatcccgc caatcgcatg gatcatcaat gctccactcg
    26401 catttacttt ttcctgtttt tctgcaaaac aaaggataga aagtaagatc cctaaaccag
    26461 gtccaggatt tgcctccatc aaaaataagg aagagtaccc gactgtttgt gagaattcga
    26521 ttcctaaagg tgtcaataca ccatgattga ttgcattatt gaaaaatagt acttttagag
    26581 gttctaagat cagactgaca agtggtaaca gattcattgt gactagccaa gacacaacgg
    26641 aggcgagcaa caagtgaaca ctatttaaaa ctggttcaat aaaaacgatt ccaattatac
    26701 aaataacgct accagtcagt cctactagta ggttgctgac aagcatttca tatccagcct
    26761 tgaatttcac atggaatcta gaaacaaatt gttcatatac gatgccggca agcggcccga
    26821 taatcatcgc tcctacgatt tgtggagcat tggaacgagc gatcaaccct aaagaagcaa
    26881 ttgcaccggc gactgcccct ttttgtcccc ctaccgattt cccgcctgaa taactgatca
    26941 agatgggtaa aagcagctgg atcatccaat ggcatacctc ttctaatgtg gtgtgcaaat
    27001 cgccagacac accttttgtt gctaatgaca agattcccca agcgataaac agacttagat
    27061 ttggcatgat catttcattt aagtgatgtc cagcttttct cagttgtttc atagacacgc
    27121 gactgctgtt gtttgctttc catttgtatt ccatcttttt caccagactt ctatttattg
    27181 atggacggat tttatgtaga aaaagcagca aacgaaaaac cgtctgctgc tttcagctat
    27241 ctgggaacta tttgcctggt tcagacgcaa tgattgctag ttcaccttgg accgcccact
    27301 cagaaacgcc atttggaata atacagcttg ttcctttttc taatggatat gtttttccgt
    27361 caacgacaag gtctcctgct ccttcgatta ccgtcatcaa tgtataaggg gcttgttttt
    27421 tgaaagatgt gattccttta atatcccact catatacatt gaaaaactca gtttctaaat
    27481 aagtgacgat cgatgaattc ccttttcgga cttctttgat ttgtaattct ggtgttttcg
    27541 cagggacagt tgttacatcg atcgattgtt ggatatgcag ctctcttgtt tgtccttgcg
    27601 catcttttct gtcgtagtca tacacgcggt aagtcgtgtc agaactttgc tgtgtttcta
    27661 aaatcatgat tcctttacca atagcatgga tcgttccgct tggtacataa aagaaatcac
    27721 cttttttaac tggtactttc ttcaaaaggt catcccagcg cccatctttg atcatctcag
    27781 ctagttcttc tcttgtcttt gcatgatgac catagatgat ttctgcacca ggttcagcat
    27841 cgatgatata ccagcattca gtttttccta attcaccttc atgtttcatc ccataggcat
    27901 catctggatg tacttgtaca gatagatcat cttctgcgtc taagatcttg atcaataatg
    27961 gaaacactgg ttctgaagga tggccaaaca attccttgtg ttctgaccat agttcatcta
    28021 atcttttccc tttgaattcg ccattttcta ctacactgac accatgagga tgcgcactga
    28081 tcgcccaatc ttctccgatt ttatcgttag ggatatcaaa accaaagact gaacgtaaac
    28141 gacttcctcc ccaaattttt tcttgaaaaa ctggttttaa aaacataggt tcctgcataa
    28201 ttcaaactcc tctcattttt catagctatt atagcattat ttcggtaata ccgctttctc
    28261 tttcaaaaag ttttcaacaa tttcatctcg acatatgaga tactccgcac gatgatcgac
    28321 aggatgtacg cgatttgaca aaaatatcaa cgcttcttgt gtttttatat caatcaagaa
    28381 aaacgtacct gtataaccag tatgaaaaag aatcgcatga ccgtcttcta catccttttt
    28441 taagtcccat cctaaagaac gatagccatt ttttttcggt gtttgatctt gcagtaaaga
    28501 atcaatcgtc tcttcttcta aaaaacgacc attacggatt tttcccttgt tcaaatacat
    28561 ttggacaaaa gtaatgctgt ctttcaaatc aataaagaga cccgcgttac ctgcatgttc
    28621 agccaaaact cttgctttcg gatcatgagt ttgtccacgc aaaacctcac ccgaaggcag
    28681 ttgttctgtg ggaacgattt ttttggaagg aaaaggaaga aataagctgt tcatcattcc
    28741 taatggttcc aatacttgtt cttgaaagat ttccactgca gtttcttggt aaatttcctc
    28801 tagcatcaac ccaagaagaa tcgtaccggc atctgtatat tttacttctt ttcctagttg
    28861 ctcacctggt tctagcttca aataagcggc gatcaattct tcctgagata gcttatctct
    28921 gtttggtatc catgttttga tatcagaagt atgtgtcagt aaatgacgca aagtgattgt
    28981 ctgattttca aattcaggaa gatatttatg aagcggttga tcgagttcta tctttccatc
    29041 ttctaataga cgtaaaacta atgaagtggt tgcaacgact ttcgtcagtg aggctgcatc
    29101 aaacaacaga tcttctgtca tcagctgctt ttcaggaacg acttgggcaa agccggctgt
    29161 aaatgtttct acttgatttt cttttatgaa ggcgactgct acacctggat aaaatccttc
    29221 ttccatcttt ttcaaaataa gctgtttcgt gtgtggatac atctttttct cctgcttttc
    29281 tatcagattc ttacagtcca agcttaaagc ttaatctatg aggaatcaac cttttttaca
    29341 caaaaaaact gcgcgaaaaa ccgcgcagtt tcccccggta tactattttc tctttctatc
    29401 tctagaataa gcgatagtat gaccgttcat aaaatgacga gacatgcgac aatcgttaag
    29461 aaagaaaaag ttaagcgtcg catcgaatgt cagttttatt cttttctttt ggctcactga
    29521 cgaaccacca ttcgattcct atagcatcat agattgtgag tactgtgtgc tttttgtcta
    29581 agaagaaatc aactgatagt tgctgcaagt gagcaatcag tccttccatg ctttcagtat
    29641 cgatcatgaa tttcagaaaa tcaagtccaa tcacttcatc tgtaggcaaa tcaattgcgc
    29701 ctccgctagc ttctgtcaaa ccaacatgga aatctccttc attcaataca tggatgccct
    29761 cttcttcatt atgtaccttc aaaccaagta catgattaag aaattggcgt tcttttgtta
    29821 agttagtaac atttaaatgg attttttcaa aataagaacc tgttgatagt ttactgaatt
    29881 tttcttcaga ttttcttaat aatgcttctt gatccattgt ttctggaatt tctctttggt
    29941 cgttcttatt ctcttcatgg taaatttcca attcattctc ttctgggtct acgagcaaaa
    30001 tccccatacg tccatgatct tctaaagctt ctttgattgg ataattcatt ttacggatac
    30061 gacaaaggat atctgccatt tcttctgtgc tagggataat caatgaaaaa cggttcagct
    30121 ttttgatcac ccccatgtga tcattcgctc taggactttc ttccagcagc aacatttcac
    30181 ttccagcttc ttctgttcct aaaatggcta attcgttttc ttctcttttc aaatcaaagc
    30241 caacaacgtt cttgtaaaaa tcaatcattt tatctcggtc tttcacccga atggctacac
    30301 tttttaaata ggtggcagcc cccaattgaa aatctttcat atttcttgct cccctctacg
    30361 tcaagcttta ttttaacgat ggattcttct tctttcaagc aataactttg attttgttaa
    30421 aaaaaatgtg agaaaattcg cattttgctt gttttttgta aattaatgag taaaaactct
    30481 tataaaaaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag ccaaacaaaa
    30541 atagctcctc ctatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    30601 ccgtttaagg aagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt
    30661 gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
    30721 acaccgttta ccaagaggtt gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt
    30781 caaaaaacag cttctcaggt ttgttgacta ttgtgacttg tgaccgagga gttggcagga
    30841 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    30901 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    30961 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    31021 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    31081 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    31141 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    31201 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    31261 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    31321 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    31381 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    31441 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    31501 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    31561 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    31621 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    31681 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    31741 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    31801 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    31861 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    31921 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    31981 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    32041 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    32101 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    32161 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    32221 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    32281 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    32341 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    32401 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    32461 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    32521 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    32581 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    32641 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    32701 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    32761 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    32821 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    32881 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    32941 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    33001 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    33061 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    33121 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    33181 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    33241 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    33301 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    33361 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    33421 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    33481 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    33541 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    33601 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    33661 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    33721 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    33781 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    33841 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    33901 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    33961 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    34021 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    34081 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    34141 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    34201 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    34261 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    34321 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    34381 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    34441 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    34501 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    34561 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    34621 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    34681 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    34741 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    34801 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    34861 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    34921 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    34981 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    35041 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    35101 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    35161 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    35221 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    35281 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg attctccttt
    35341 ctttcaagat gacacattgt catatgacgc tatgtcatta taacacctcc tacctgtttt
    35401 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    35461 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    35521 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    35581 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    35641 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    35701 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    35761 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    35821 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    35881 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    35941 ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt tataacaatc atagcaataa
    36001 aaggagaagg taagcatatg gcatctgttt tagtagtcaa aggtcatccc ctaactgcag
    36061 aagaatcgcg cacagttaag gcattgacgt cttttttaac aagttataaa gaaaatcatc
    36121 cagatgatga agtgactgtt ttggaacttt atcgcgatga tattccggaa atcgatgaag
    36181 aattattatc aggttgggaa gcattacgtg ctggtgcaga attcacttcc ttatcagaaa
    36241 gtcaacaaca aaaaattgct cgtttcaatg aattgactga tcagttcctt gctgctgata
    36301 aaatcgttat cgcaaacgcc ttatggaatc taaatatccc aacaaaatta aaagcttggt
    36361 tcgataccgt caatgtagct ggaaaaacgt tccgctatac agaaaacggg ccagaaggat
    36421 tagtgacagg taaaaaagca ctgcacatcc aatctaatgg cggtgtatac aacggacaag
    36481 actttgcctc tcaatatgta aaaggtattt taaactttgt aggcatcgat caagtagatc
    36541 agctattcat cgaaggaatc gattatgacc ctgaccgtgc agatgagttg atgcaaaatg
    36601 cattagataa agctgctgct ttaggtaaat cattttaaaa attgagataa atgcatacaa
    36661 aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc ttggatattt tcccatctgt
    36721 ctctttttgt tattctttct gctttttcaa cggattttct tcataaagag aagagattgt
    36781 tgtgtaccat tcaaaaacat gagtaatcac tactttagct gctgcataaa caggaatccc
    36841 caagatcaca ccgatgattc cgaacaattt cccagaagtc agcagaacta ataaaatcgt
    36901 tactggatgg attgctaact gattccctag tactaatgga gagatcaagc gtccttctat
    36961 tgtctgctct actgcaaaaa cgatcagtac tttgatgatc atgaatggac ctgctacaat
    37021 ggctaaaaag atagccggaa tcatcgctaa aaatgatcct aagtaaggga tcaggtttaa
    37081 aaaacccgca ataatcccca acgtgacagc atactctaaa ccaatgaccg caaaaccaat
    37141 cataaacatg attgccacag caaaagccac agttagttgt cctcttatat aagaagaaac
    37201 ttgactattc atttcactca gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa
    37261 tttgacaaaa taaggtgcta attgtttacc gtctttcaat agataaaata agataaacgg
    37321 catcgtcagc agagccacta aaatagtcgc taccgcgccg acaaaacttc caagactctg
    37381 aactgtactc ttagatatat cttggatcat atcgcctgcc gaattcatga acttatcacc
    37441 tgctgtttcc aactgttcgc gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa
    37501 tttattatca atcgtatcta catattgagg gaagttactg atgaagctca ctgtttgttc
    37561 ctgaatctta gggatgatca ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa
    37621 cagcccaatg atactgtaaa gccttggtac ttttcgcttt tcaaagtaat caactaccgg
    37681 attcatcaag taatacaaga ttcctgctaa aatgataggc agacctacga ttgctaaaaa
    37741 ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat aaaaagacga tcagcaatat
    37801 taacaataca acaagtaagg ctgtgactac ttgattattt aaaaaccagc gccaaaacca
    37861 cgataccggt ctgttcttct tattttttcc ttccataatt cctcctgtta gttataaaaa
    37921 atttggcttc ccaaaccaat agcaggcagt ctatgcggat gaagatagat tccatttgca
    37981 atttgtcctt cttctggaca agcatcaaaa atcaatgata cgtgagctac tgtctttaaa
    38041 ttcccattgg ccatcggacc gacataatcg atcgtatata cctgatcatc aaaagcaatt
    38101 gttccgcctg ctttcaaaga aaaaggttcg tcttttgata cttcttgaat caccgaatat
    38161 tttcgtaagg cagctgttgc tgttttatcg aacaaaatca gcatgggttc ttccttgttt
    38221 attgcttctg tcccaatttc tgtaatcttt gcctgaatca ttgcactctc tcctttaatt
    38281 gctttcaaaa acagtataac acattctcat tctttagccg tgtggaataa ttatgtgata
    38341 gaataaaaac gaaaactatt tcattacaat taggaggaat cagctcatat gatcgaaaag
    38401 ataattttag gaacctatac gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat
    38461 acagaaaaag aagaattagc caacctttca ttagctgttg aagaaaatag ccctacctat
    38521 gtagcaaaaa gtaaagctgg caatctttac acagttacta gtgtggatgg attaggtggt
    38581 gccggtgcgt acgataagga ttttcatttt ttgaatgcgg taacagagtc tggcgctcct
    38641 ttgtgttatg tagcggttga tgaagcacgt caattactat acggggcgaa ctatcataaa
    38701 ggtgaagtaa atgtctacca tatattagga gatggcgaac tgaaagcagc agattcaata
    38761 ttccatcaag aagcgacagg tcctcataaa aaccaagatc atgcgcatgt ccactatacg
    38821 gatctgactc ctgaccaacg attagtcgta tgtgatcttg gtacagatcg tgtgtatact
    38881 tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta ccttcactgc agagccagga
    38941 acaggccctc gccatcttgt ctttcatcca aatggacaat tcgcttattt atttggtgaa
    39001 ttagacagca cagtccgcgt gctttcttat aataaagaaa caggtgcttt tgaagaatta
    39061 cagaaaatat cgaccttgcc agaagaattc gacggtgaaa atggaggggc agctatccgc
    39121 atttcaaatg atggcaagtt cctttatgct tctaaccgag gacataactc gattgctgta
    39181 ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc aaatcatctc aacagaaggg
    39241 gattttcccc gcgatttcgc actagatcca agcaataatt ttgtcgtgtg tgccaatcag
    39301 aacaccgata atctgacatt atatcgccgt catcctgaaa caggattact ggaattactg
    39361 aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag
    39421 gagaatgaaa tgaatcagca agacgtatta gacagactac gggaagaact aaatattcct
    39481 ttctttgatg ggaaaatcga agataaagag tatagcgaag aggaatatca gaaactcaaa
    39541 gccgatttac agaactactt tgaacaatat gtacagaatg tagaaaacta atcagctttc
    39601 catactatct gaaaagaaca tcaggacaag acttttccgt ctatcctgat gttcttttct
    39661 acaaataaaa aaccgccgag atggttagac tcgacggaaa aggagtattt actcgatttt
    39721 gagtaagtac aaggttgttg ttggttatag gtatgtcatt atcataaagg attttgatca
    39781 ctctatcata aaggtaatat tacatttata ttagataact ctagttttta ttgttatcaa
    39841 agatttcttt tattttttca acaacaaatt agtcattttt tggatggcag ctgtcaattg
    39901 gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat agatcttcat ctacccctga
    39961 ttcaagatcg atactttgga tcatttcttc acagcctgct acatatgaaa gataagcttt
    40021 ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga cgcaaagtac tgactttcat
    40081 aagcattgcc cgatattttt ttgttccttc ttcaaataac aggatgatct cttcaagagt
    40141 cgatttgtcc agttcacttg tttttccttc atcaattgcg tttctgatca cttcatattt
    40201 cggatgcatt ccttctccga ttgcttctgt ttccttgacg atttgatcga tggactgcac
    40261 ataatagcca atattcggta acatgttcgt tttcctactt tctccattgt tgttattatt
    40321 tattctactt gtttttatcc tcaaatgaca agtacttgaa aaaatttaat ggtttctata
    40381 cagctcgcca ttcttattcg tgctcaaaga aaccatctgc taaactatat tgtttccctg
    40441 cgacaatgtc atattggatc aattgatatt cgtcgtaaat ctcttgttgc gagcgattga
    40501 attgcgcttc tttataccac tgtccgtttt gatagtacag ttctcgttca aacgccggaa
    40561 gttcctgttc taatgataaa agcagctgat aaaagccatt tgttgtctga ttcgtctgtt
    40621 ccattagatt cgctgcaaaa taaaacggac tggtgattgc atcgtgagtc gtttgctttt
    40681 ctaattctcc tttactgtcg aacataagaa attgtgtttc atgcaatgtt tgtttgtcat
    40741 tttttgcttg gatcgtatct gaatagatcc ctggaagatg atccccccaa aaaacaacta
    40801 aagtccttct agaaagattc ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat
    40861 aggaaatatc ttgcagataa ttctctaagt caagtgtccc acttccatct gaagtttttg
    40921 caggatagtc tagattatca tactttccat cgtagggcat atgtgtctgc atcgtgacta
    40981 aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt cattacttct ttgtaagctg
    41041 actcatcaga aatatatgga ttattctcta tcgtatccgt ataggtcatc gtacgctcgc
    41101 tgatgaactg gtcaaatcct aatgtctgat aaacatcctc tcttttatac atcgaagtat
    41161 tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt taacgctgaa acgatagaag
    41221 gaagctgatc catttttggt acaagcatcg tgtaaggggt ggtgagttgc gcattaaata
    41281 acgccataga aaaaccagtc aatgcttcaa attcgatatt tgctgttcca ccgccatagt
    41341 tttgagaaag catattgcca ctatatgttt gatcagctac ttcataataa tctgccaatg
    41401 gctcaccaga tacttctacg ccgtttaatc gactaggatc tgagaaactt tcactcatca
    41461 cgaacacgat gttcggcgac tcttcctcta cagttttatt tttttcatcg gctagcttct
    41521 ggtatttttc agtgatttct ttgatctttt ctttcgaata tccttcaggt tcatccattg
    41581 gttctacctt cagattgtac aaaaacccac cgataaaacc agtgttatag tagttcattt
    41641 tctggctgta aggtatccat agtgccgttt tattatatgc ttttcgcaac aaattatcgg
    41701 gattgttgaa attactgata taccctagca gtccaattgc cacaagcagt gtcgttaatc
    41761 ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa catgtaccag caaaacaatc
    41821 ctatcacggt tcctgcggca gctaagatca ctgtaaacag catagtgccg gtcatatctt
    41881 tcagcaatcc gatttcagtg atcatcttca aatcgtctgg ataaatcggt tcttgccgat
    41941 aactcatttt catataattt gcaaaaccta atacaccgat ggctacgaca tacgtaaggg
    42001 aacctaaagg gacagatcct gctagtgcaa tcagaaaaat aagaataatc agcaagacga
    42061 gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta
    42121 cggatagatc attttgacac cactgcaaaa ataaattact aaaaacaaca agaaaaaaga
    42181 tactgatgat tgctgctgtt ccttttaaaa tctttttctg tttggtcgaa agtctgctta
    42241 tatatctttt caaatgcttg ctccttcatt tctatcaata aaagaatgtt gcttctctta
    42301 ttttagttgt acgaaattcg aaagtaaaga acggaaagaa gaattaatga aatccaaata
    42361 ttctctttct ttatggcctt tctggtttct ttaataagcg gacctttcgt actccagaaa
    42421 ttttttctag ctgttgtttc acttgattga gctgttcaaa atcagtactg tctatgtcaa
    42481 tcaatatgta gacatgctca ttcttatggt tacgcgatat atctgcgatc gctagctcgt
    42541 tctcattcag cagagaaaat atctctgcta atatattggt tttcttttga taaaatatag
    42601 tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg ataattgacc gcttctctaa
    42661 ccgtaccgaa caaaagataa tcttttaagg cttcaactgc caaacgtccg ctgtctgcca
    42721 aagcttcctg agtcgatccc cctagatgag gcaacataag tatacggtca ttttctaaga
    42781 actcttcttc tggaaaatca gtcaagtatt tagccagatg ttgtttttcc aatgcttgca
    42841 taacagcata tttatcaaca atttcacttc ttcctacatt gataagtaca gcatcttttt
    42901 tcattttttc tatattcgcc tgatcgatca atccttttgt atcttctgtc aacggcagca
    42961 tgactacgat gtaatccgag gtagaaagaa ctgtttctaa atctgcttgc cgaacataat
    43021 ccaactgggc atcgcggata gaataaccca agacatccat ccctaagcta tagcaactta
    43081 gcgctacttt tgtgcctatc gctccaagac caagaagacc gatggttttg ccttgcaatt
    43141 ctcgtcccac ataagcactt cttttgtttt ctgcctgttc taaaatatta ggacctgtta
    43201 gtgtttgtac catacgactt gcttcgatta tcggacggga agataagagc aaacagcaca
    43261 aaacaagctc ttttactgca ttagcattta cacctggtgt attcatgacg atcgtcccgt
    43321 tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac accagctctt gaaattgcta
    43381 gcaaatctgg tgtaagccat tcatctttca ctttcgtact acgaataact attccttcgg
    43441 ctggcaaatc atctattatt ttgaaatatt ccccttgtaa cttttctatc tcttgttctg
    43501 gaatagcatc aatcaaatga acgtgataca taaaaccccc tccttactaa caagtatctc
    43561 gaaatttctg caattagaca actgtttacc atttgacttc ctagaagcaa aaagagcagt
    43621 tagagcagag taggctgagt gataaaaata aggctatgac aagttactgc cataagcctt
    43681 attttcgaga gcaaaatatt gagggtgaga tatcttgata aatggtgttc cgttagctga
    43741 cattgataat aagactaaat ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt
    43801 atttcttaaa actaagtggc tttttcacaa cctcttttta tctaataacc actgtttcca
    43861 actcaactaa ttttgcccac gtttcaattt gatctgttgt tagatttagc gagacaacgg
    43921 tatgatgacc gccacctgcc tgaatccatg aatggacacc ttcatggaaa ttcggtttag
    43981 ttttccataa cacacgtgca acaggtaaat taggtgcttc agtcgttggt tcaaaggctt
    44041 ctacttcatt gatcagcagt ttataatgcg taccgaaatc tgccatcgat acgactaccc
    44101 cttctcctgc tttcccatca aatacaagac gcgccggatc ttctctatct cccattgata
    44161 aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt cggatctact tccatcatat
    44221 gggattccaa gattgcttct tggccactag ctagctcgta agtataatct tccatgaatc
    44281 ctgtatcttt ccctcgagcc atgattttca gcaaccgatc aatcgctgca gttttccagt
    44341 cgccttcccc agcaaaaccg tatccttctg ccatcaaacg ttgtacagct agtccaggta
    44401 gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa agcgttatag cctccttctt
    44461 ctaagaaacg gcgaataccg atttcctgct gagcttgtac ttttacatgt tcctcccatg
    44521 tcttttcttc ataatcacca tagtcgaagg tatacaattc cttgtactct tcaaataatt
    44581 gatcgacttc ttcgtctgat acgcgatcga ttacttgtac aagatctccg ataccaaaat
    44641 aatcaacagt ccaaccaaat tgaatctgtg cttccacttt gtctccttct gtgacaccca
    44701 cgtttctcat gttgtcacca aaacgagcca cttttattcc aaaactttca ttataggcta
    44761 ctgcaacatc catccaatca gcaatctctt tttggatctc aggtcgtttc cagtagccaa
    44821 cgacgatttt attttgttta tttaatcgag cattgatgaa cccatattcg cggtcaccat
    44881 gagcggattg atttaaattc atgaaatcca tatcgatcgt tttccaagga atgctttcgt
    44941 tatattgcgt agctaaatga agcaatggtt tttgtagaag ctttgtcccg cggatccaca
    45001 tttttgctgg tgagaaagta tgcatccaag tgatcacacc tgctacttct tctcgataat
    45061 tgacctcttt catgattttt gtgatcgtat cagcagtaac tgctaattct tgaaggcgga
    45121 ttggataagg caattggcca ttttcattca gttcatcgac catttcttgt gcatgtttct
    45181 ttacttctcg cagtgcttct tccccatata aatgctgtga tcctacaaca aaccaaaatt
    45241 ctttttctcc aatattcaac ataattagcc actcctttta tttgttttgt ccgtaatacg
    45301 cattttttcc gtgttttcgt aaataatgtt tatctaaaat ccgttgaggc agctcttctg
    45361 aaaatgaatt gagctgtctg gtaaataaat tcatcttgca tacttcatct agtacgactg
    45421 cgttcattac cgcactttgc gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa
    45481 gtacccctgg aattgctaag ggatcaattt tgcgttcttt gaatgtttct atgataactt
    45541 ttcctgtttc atattcgtac ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag
    45601 gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg
    45661 cttgtgccca gatcgtcgcc catgtcgagt gagtatggac gattcctccg atttgaggaa
    45721 atgaacgata taaaacagca tgagtaggcg tatccgaaga gggattgagt ttaccttcaa
    45781 tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc tgtcatctct tcataactga
    45841 ctccgctagg tttgataacg aaatatcgtg tttcaggatc aaaagcgctc acatttcccc
    45901 atgtatattt gacgagtccg tgtttaggca agtcaagatt tgcttggtat acttcttctt
    45961 tcagttgttc cagcattttc tatcactcct ttttcttgca attgatgaat cgctgttttt
    46021 tcaatgtcga tcccctcacg gtaacgtctc ataaatatct gatagcctgc tatttcttct
    46081 gctgaaggtg tgatggtgat tccttggtct tgtccaaaca cgtcttctgc caagaagttt
    46141 tctaaagact tcccatgtcg atccatcata taggctgcta ataacgcgat tccccacgct
    46201 ccaccttctc cggctgtgtc catcactgta actggtgcct ccatagcaga tgctagaatc
    46261 cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc ccactaaacg atcaatttgg
    46321 acattctcag atttcaatat atccattccg attctcattg caccaaaagc actggctaaa
    46381 tggatccgca tgaaattcgc taaatcaaaa cggctacctg gtgtacgtac aaataatggc
    46441 cttccttcat ttactcctgt aatgttttca ccggaaaaat aaccataaga aagtaatccc
    46501 ccgcaatccg tatcgccttc tagcgctttg ttgaataata ccgtgaataa tttatctctg
    46561 cttatctcca tacccaaaga attggtaaat tcttcaaaaa tactgaccca agcattgata
    46621 tccgaagagc agttattcgt atgaaccatg cctactagtt ctcctgaagg tgtagtgacc
    46681 atgtcgattt ccggatggac attttctagt tctttttcta atacgatcat agcgaaggcg
    46741 gaagtaccag ccgaaatgtt ccctgtccct ttggctacac tgttcgtagc gaccataccg
    46801 gttcctgcat ccccttctgg cggacaaacc gggattcctg gtctcaaatt tcccgtaggg
    46861 tcgatcagat aagcccctga ttcgcttaac ttgccagctt cttctcctgc tactagaact
    46921 ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa
    46981 atatttttca tttcttgatg gtaggttttc gttgaaggat cgataggaaa catgcctgat
    47041 gcatctccta cgcctagtac tttctcacca gtaagctgcc aatggacata acctgcaagc
    47101 gttgtcaaaa aattaatttc tttcacatgt tcttctttat tcaaaataga ttgatacaaa
    47161 tgagcgatac tccatctctg cggaatattg tattgaaatt tttccgttaa tattcgttct
    47221 gcttcttcag tgattgtatt tcgccaagta cgaaatggta ctagtaactg ttccttgcta
    47281 tcaaatgcta gataaccgtg catcattgca ctgaacccaa tcgacccgat tgtcgtaaga
    47341 ttctcgccat agatttcgaa tacttctgct gccatttttc ggtaactgac gcgcagtcct
    47401 ttccaaatat cttctaatga ataggtccag ataccatttt ctagtttgtt ttcccactca
    47461 taactaccag cggcaatagg agtaaaagat gaatcaatca atacagcttt gatcctagtt
    47521 gatccaaact caatccctaa agatgtacga ccatctatga catctaacat actttgcgca
    47581 tttacttcct tcataatgat ttaactccct ccttctcgtc aaacctgatc aaaccgtctt
    47641 tttagtagcg cttacaatga ttattgtata cttttgtacg tacatgtcaa gcagttattt
    47701 acaagaatac aataaaaaac acttgttttg tacgtacata agttataaaa tgaaattgct
    47761 tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg agagaatact tatgaaaaaa
    47821 gaaaaaaaag tttcctcatc gtttatttat ttttttggcg catttggcgg cattctattt
    47881 ggatatgata ttggtgttat gaccggcgcc ttaccttttt tacagcatga ttggggtttg
    47941 gcaggtaaag cttctttgat aggatggatc acgtcttcgg tcatgttagg tgcgatcctt
    48001 ggcggctctt tatctggtct tctttctgat aagcttggac gacgaaaaat gattcttctt
    48061 tctgcactta tttttatggc tggttctgtc ctttctgcat ctgcccctca caatggttct
    48121 tattttctga ttgcagcaag gattcttcta ggtttagctg ttggcgctgc atctgcccta
    48181 gttcctgctt atatgtctga aatggctcct gctagactaa ggggaagatt gtcagggatc
    48241 aatcaagtga tgattgcttc tggcatgctc ctttcctatg ttgcagatta tttgctaaaa
    48301 gggttgcctg aaacgatggc ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg
    48361 attttatttt tcggtgtttt agctctacca gagtcaccgc gttttcttat gcaatctggc
    48421 cgcttggaag aggcaaaaag agtattgaac tatattcgga caccaaaaga agctgaacag
    48481 gaattcgaac aaatccagct gaatgtcaaa caagaaaaga caaccggaac ttcttggcat
    48541 actttgtttt tagaaaaata tcgttcctta gtcattgccg gtattggtgt agcagtattc
    48601 cagcaatttc aaggagctaa tgctattttt tactatattc cgctgatcgt agaaaaagcg
    48661 actgggcatg cagcgagcga tgcattgatg tggccgatca tccaaggaat cattttagtt
    48721 gctggttctt tgctcttttt ggttattgca gacaagttca atcggcgaac tttattgaaa
    48781 ataggcggta gcgtgatggg actttctttc atcctaccag ctgttttagg aactgtactg
    48841 gatgctcata caaattcgtt gttgattctc ttgtttttat gtatttatgt cgcattttat
    48901 tcttgcacat gggctccact gacatgggta atcgtaggcg aaatattccc actagctgtc
    48961 cgcgggcgag catctggcct agcctcttct tttaattgga ttggttcttt cctagttggc
    49021 ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa tcgttttcgg catttttggg
    49081 atcatctgct ttttaggggt tttatttatt caagagatcg taccagaaac tcgtgggaaa
    49141 tctttagagg aaatcgaaca aagtgcatct aaaaaaactt atcctaaacg tatttctatc
    49201 catcattctt aaagaggttg tgacagaagt ggtcagcttc aagaaataag aaggaaccgc
    49261 ttagtttcca gtcacaagga acaatttcaa aaaacagctt ctcatgtttg atgaatattg
    49321 tgacttgtga ccagttggta ggagaacacc gtttagtgga gaggtggtga aagaaacgtt
    49381 cagccttgag cgatgacaaa agctctcaca aacattgcga cgagtaaccg taggagcaat
    49441 aagaggcggt aaaaacccgt ctcttatttt tcgttttctt tcactgtttc ccgttcaact
    49501 agtacaggtt catagagaat atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc
    49561 caccgtgcgg catcttgtcc caacttttct tttggatgcg tcaatgttgt caatttttta
    49621 ttccctaaat ggcttaaaga agaatcgtct gttcctacga tcgataggtc atctggaata
    49681 tgatagccat tttctgtcaa cttatctagc attaaactag ctatttgatc gttatagcaa
    49741 atgatgccat ctaaagcttg ttcttctttc aaccgttgta ctgttttttc agcgattgtt
    49801 tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca aaaaacgcac attatactgt
    49861 tcacacgcgc tgataaaacc tttcatcctg tattttcctt gaagatcgtc gatttttgtg
    49921 atgaacaaca gacgctgatg tccttttttt atcaaatatt ctgctgccat aaaaccgact
    49981 ttcacatcat ctaaacaaat ataagaggca tttaactctt cataagcagc attgatcatc
    50041 accatcggaa tgccttgttc tctcaaagca acataaagtg ctaagtttgg gttatactga
    50101 ttacttttcg taggctcaac gatcaaacca tctaccccat aagcaatcat tttcttcaaa
    50161 cactctttct ccaattcgtg atcgttattc gtactggcta aaagcaatga atacccagat
    50221 tctcgtaatt cattttcgat tcctcggata atcgacggaa aaatataatc agagagatat
    50281 gtggtgatga caccgatcgt ttttgttctg ttcaatggtg ttttttcttt cttatagctg
    50341 tcatctacat atgtgcccga ccctttttct ttacgcagat agccctcatt taccaacaca
    50401 gcgattgctt gccgaatcgt atgtctgctg acgtcatata tttcttgcag cttgaattct
    50461 gagggaatgg ttgtatttac ttcgtattcc tcacgaagga tcttttgttt tatctcttct
    50521 gcaatatctt gatattttgc catttgcctt gccccttttt atttgtacgt acatatatca
    50581 taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata
    50641 cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt
    50701 cgcgtgccga ttaggattgt ctaacggatc ccctttttgg ttcttctctg gtcgagcatg
    50761 atagatcaaa atatcaaagc acccgtcttc tgaaaccgta aacgagttgt gaccaggacc
    50821 aaattgctta tttttttcag aagaaacgaa tactggctcc gataatttat gccatgattc
    50881 ttttaataaa agattcttgt gctcatcagc ccacagcagg cccatggcat agttttcatc
    50941 tgttgcacta ccagaatacg taatgaatat tttcccattg cgagtaagga ctgctggacc
    51001 ttcatttact gaaaaacccc gtttttccca ttcatactca ggtatagaaa ggaggatctg
    51061 cttccccttt aacgtccaag gattatccat ttcggataga taaagattag aattcccagt
    51121 aatttcagga tcttgttggg cccacacata atatagcttt ccttgagaag agaaagttgt
    51181 tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt cccttttcta tccaatctgt
    51241 attcattgga tctatatgct gattttcaag tacgaacatg cggtgatgat gagattcatc
    51301 acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac cattttccat tgatataatg
    51361 gacttcaggt gcccagatca attcactcat cggtccttct tcatgagcct gccatacaat
    51421 caggcttgca gcatgttcta attcattcag tgactttgcc cttcttaatt caatcacttg
    51481 gtagcctgga accgatcctg taaaataata ataaccatct gtatgtttat atacccaagg
    51541 atctgcccgt tgccgaacga taggattatt ataaagtgtc ttcatcataa caaatttcct
    51601 cacattttct ttattctaat tacattccaa gagtattttt ctatccctac atttaattcg
    51661 tcatgttttt ctattgaaaa atagtttatt tcaggtttta ttttttctgg ttcttcacgt
    51721 gtatttttgt ccaacagatc gctactggtt aatgtgtagt gctctccttg atctagcaaa
    51781 ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg tgcgattgat caagaaaata
    51841 gtcagctgat tgccgttatc tacgatcaca ctgtccacat aaggaacatt ttttatcttt
    51901 tcacaatcat aaacagggca aatgatttgt tgtttcaata cttgaccttt agcatatttc
    51961 gataagtgtt gcatcacata ataagttgga ttcctccatg cttgtccttt agcatctgtc
    52021 agtattaaag gtattgtgtt tactaacaat gattgacaac taatctttac gcgatctgcg
    52081 tgtcgtaaaa tagccagccc taatgaacca aataataatg tatcttccat agtaaagtaa
    52141 caccaatcga tgggactacc gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt
    52201 acattccatt catcaaacgc tagatacatt gttttctcac ttctcaaata tccttttaca
    52261 taatcacaag tagcaacgat ttcttcaatt tgacgatcga atcgataagc ttttgcaagg
    52321 tatgtcgctg tatcatcacg ctcattttta ggaggtttag tcaagtcttc ttcatcatac
    52381 ttatcaatat agttatgcaa agctaaataa tctacattct cataagcttc ttctaatgcg
    52441 atacgatccc attcagggta cgatggtaaa cgtggcgtag agcttccaac taagattgtt
    52501 tcaattgaat catccactaa cttcatgact tttgaggctt catttgctaa cctaccatat
    52561 tcataagcac tcttagcccc cacttgccat ggaccgtcta gctcattccc taaacaccat
    52621 aatttaacgc caaaaggctt tttttgccca ttttttattc ttaaatcact ccagaaggtt
    52681 ccttttggaa aattacaata ttctactaaa ttttttgctt catcaattcc ttttgttcct
    52741 aaattcaccg tgaatatcgg tttcgcttgt acatgactga tccatttaaa aaattcgtgg
    52801 agtccaaaag aatttggttc tatacctcgc catgctaaat caattttttt tggtcgttgt
    52861 tctttcctac ctacagtatc cacccaatca taaccagatg taaagtttcc tccaggatag
    52921 cgaatgacac ctaattctaa ttcctttaca taagagagga catcttgtct aaacccgtca
    52981 ccatcagctg tttcatggcc cggttcataa atccctgtat agacgacact tcccatatgc
    53041 tcaacaaatg agccaaacag ccgagaactg atctctccta ccgtaaaatc ctctctgatt
    53101 tttactttca cttcttcgtc caacagcttt ccctaccttt catttcctat agtcaagata
    53161 atagatacaa gtttccatac gccaccagca aagataaaaa taaaaggagg gaaaagaaat
    53221 atgagcagta caaatcctaa taacaatacg caacaaagaa tatttctcaa aaaatcaaaa
    53281 aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa
    53341 atcacatata tccaggataa aagaaacatg gaataaataa gtaaaactac atatccacta
    53401 attgaaagta cgattgtctg ttcttctaat aattgattaa caagaaaata aataatgatc
    53461 gttaacagac taaggaacaa gttcttacca aaatgttgct ttattccttg aaagaaaaaa
    53521 taaatgatat ttcctgatcc ctcacctttc cattgcgcca ttacataaaa aacagcacat
    53581 gtagcaggca cgatagtaat aacaggcaaa cacacgatta cccagagaat acctaataag
    53641 atgtaatcag aaaccacaat caactgtcca taaagtttag tctcaactat tttcatcttc
    53701 ctcactcttt cattccagac atagctgttc cttgaacaaa atatttctgt gcaaaaatat
    53761 aaaccactag taatggcagc attgaaataa ccgttccagc cattacaggt ccataaaaag
    53821 aaatgttctg ccctgtaaat aaagctaacc ctgctggtaa agttctcatt tttgaactac
    53881 ttgtcaatat caatggataa agcaagtcat tccaactatt catcagcgtg aaaatcccta
    53941 atgataaaag agtaggtttg ctcaatggca acatgatttt caaaaaaata ttaaactcac
    54001 ttaatccatc aatgcgtgct gcctcttcca aagaatctgg taaagtaata aaaaaggaac
    54061 gcatcatgaa gataccaaat gcagttgtca tacgaggaat gatcaaacct gcataagtat
    54121 caagcatgcc aagcaaattg acttcaataa atagaggaat catgaagact tggaatggga
    54181 tcatcattgt cagcaaaacg aaatagaaca aaactgattt tcctttgaaa cgcatacgag
    54241 caaatgcata ccccgcaaga gaatcaaaga atacagaagt gatcactgtc cctccagcaa
    54301 aaatcactgt atttttgatg taatctaata aagggatcgt tgtccaaacc ttaatatagt
    54361 tatctagggt atagctatct gcaaatattc taggcggaaa tgagataatg tctttttcgt
    54421 gtttaaaaga agaaataatc agccacaaaa aaggaaacgc aataaccaac aatataacta
    54481 gcatactaat cgttcgaatg atttttttac cactaactgc ttttcgacta ttcacagtaa
    54541 ctgtaatcga gctttctggt gccatacagg tttccctcct atttcatttg tttttctttc
    54601 cgtgacatgt acacattagt ggagatagct atagttgcaa caatgataaa caaaaccgaa
    54661 gacagtgctg atgcatagcc caattcataa ggtgctgtaa atcctcgact gtaaatatat
    54721 tgaacagctg tttctgtctt aaattgtggg ccaccagcgg ttgcaacata tacttggtca
    54781 aacacttgca tcgaaccaat cagattcgtt aaaatacaaa aacctaagga gggaacgatc
    54841 tgaggtagcg taatgttaaa aaactgctgt cgatttgttg caccatccat ttgagcagat
    54901 tcatacaaag agggagagat tccctgaata gcagtcaaga gaatcgtcat agtaacacca
    54961 aaattcttcc aaacagtcat gatggctacc gtcggcatag caagggttgg atcgcgaaaa
    55021 aattgaggga tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc tacattcgga
    55081 tcaagcaaca tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa
    55141 aatgtcgaac gacaaaaacg attgaatctg gtatttttag ataatgctac tgctgctaat
    55201 aaaccaacga taatttgtgt tggcgtttcg agtacggtga aatataaaga atgaatcaac
    55261 gaattaactg ctcgttcatc actaaatata cgtgtgaaat tttcaaaact tacaaaattt
    55321 cttgatgtaa agaaaatatt caaatctgta aaactaatag caaatgttcc taaaagcggt
    55381 atgacagtaa ataataataa aactaaaaaa gatggagtta aaaacaaata cgcagcacga
    55441 tcaggacgat tccaatattt ggtgaatccg ttcatatagt ccacctcatc tttcattttg
    55501 ataaaatata caatcttggt ttaaacgaat ctgaataaga tatccgaaca atcgctgtga
    55561 tttttgcaat aacacctatc tgcaaatact aggtcgatca ttcagatatc tgtatcttgc
    55621 caacagtcta tcctgtataa agttctgtac tatttttgca gtaattgatt gatggcatca
    55681 tctgcatcgt tcatgagtgt ctcaggatct tctcctgcta ataatttttc aatcagtgga
    55741 ttgatgatat cgttattgat cgcagagatt tgggtaaaac ctggtaaata tggctctgca
    55801 tagtccattt gtttcgataa ttcagataca ataggatcat tttttacttc ttcatctttt
    55861 gctactgaat ccaagtaggc aggaaaacca ttttcgatac tccatttttt accaatttca
    55921 gttgtattcc aatatttcaa aaactcatag acagcttctt ttttattatt atctgtactt
    55981 gccggaatgg catatcctac gccatctaaa atagctgttt ttttgtctga atcactccct
    56041 tgtggtaagg tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt atttaaccat
    56101 ggcccattga tttcaatggc tagttgacca gcattcatta aattgtccat ttcggcgcca
    56161 gaaatcgatt ctggaccagt cttgtctaca tggatcattt tttggatcgc ttttaacgtt
    56221 ttaaggttct caggagacgc aaattcagac ttagtataat catcgttcac taatttaccg
    56281 ccatttgcta acatccagtt atataacggt gcagttccat ccttattgaa gacaaaacct
    56341 gatacatttt tactactatc agttaatttt ggtgccattt ctgtcaattc ttcccaagta
    56401 gtaggtggtt gttcagtatc taatccagct tgtttaaaca aatcattatt ccaatacata
    56461 tacatgcctt gtacctgcat ggggacaaaa tactgtttac cttctacttc acctaattca
    56521 atagccgttt ctttaaaatc agatttatct actccatcat attcccagaa atcgtctaat
    56581 ggttgtaccg ctccgttttc tacatactgt gcaaaatctg gataatttaa tgcaataaag
    56641 tcaggtgctt ttttagaaga aatcgctgta ggaagttttt cattcaaatt ggcccaagtc
    56701 atgatatcca tttcaatttt gatgttttta tcatttgttt tattaaattc attaacgatt
    56761 tcttgcaata tttcaccatc agatgcagta aacccattcc aaaaagtcaa agtcacttct
    56821 ttatcagaat cagattctgc tgaacttccg ccaccgcaag ctcctaaaat acctgttgca
    56881 gctaccaaca aagagccaat taaccatttt ttacctttca ttttttgttc ctccttcatc
    56941 tgataaacga catctgacca cattccaaga tagcggtttc aataaacaat tgattttccc
    57001 atcttcagct tttacatttg ttgaatgact tggttttact tgttcacttt taggaagatt
    57061 agttgctttt gtatcaaatc cactcatttc agaaaaatca atcactccct ctaatgataa
    57121 ttcagacaag tgaacagtta gattcatttc ttcttctcca cggttaactg caaatatgac
    57181 aagttctttg tcatttatta cggctatact ttctaaatac ggcacttctt caaaatcttc
    57241 cgtcgcatag tttgctgaac gaatttgtgg gcttaacacc tctccttgtc catacacagc
    57301 agcttgcata aatggataat agatcgtctg tctccaagct tctccgtttg tttctgtcct
    57361 gattggtgca ataacattta ccaattgagc aagacaagca atctttactc tatctgcatg
    57421 tttcaataaa gtgattaata gacagcctaa tagtaatgca tcttcaaaat tatagttatc
    57481 ttctagtaaa ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg
    57541 ataccaaata ttccattcat caaaggacag gttgatctgc ttcttactat gtttcttcgc
    57601 cttgactgta tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg
    57661 tgcaagataa tgagaaagat catttttttg gttgccataa tattgatgca gagacaaata
    57721 atctatttga tcgtacgctt gatcaagaac tgttaactcc caatcaccaa atgtaggcat
    57781 ttgccgattc gaacttccgc ataaaaccac ctcaattgaa tcatctacga gtttcatcgc
    57841 cttagccgtt tcctctgcta accgtccata ttcttccgct gttttatgtc caatctgcca
    57901 aggaccgtcc atttcattcc ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc
    57961 attcgaacgc cttaaatcac tccaataagt tcctttagga aaattacaat attcgactaa
    58021 attacgagct gcatctactc cacgagtacc cagattgaca gccatattga tttcggcccc
    58081 cacatgctga caccagttat aaaattcatg aatcccaact tcattcgttt caattgtccg
    58141 ccaagctaaa tccaaccttc taggtctatt tgctatgggc ccaatactgt cttcccaacg
    58201 aaatcctgac acaaaatttc caccaggata tctaatcatg ggaatgttca attcttttac
    58261 caagtgaatg acgtcttggc gaaatccact tttattagct gatggatgat ctggctgata
    58321 aatcccttca tatactgctc gtcccatatg ttcaataaat gaactgtaca accgcggatc
    58381 aactttgcct acacgcttga cactactaat caacattgtg gcttccataa ctgacctcct
    58441 ttaatttata catgaataat acacctcgtt gaaagcgttg tcaataatat aaatgttaaa
    58501 cttacttatt ttttatcgat tttaattaac caaaatatta ataatgatta aaacaaataa
    58561 tttaatataa cttttattaa caaatatgtt aatatataag aaaaaccttt taagaggtga
    58621 tatatatgca actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta
    58681 aaacaaggat caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg
    58741 caaaagaact tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag
    58801 caaacatcat caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata
    58861 aaatcgatat cagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga
    58921 tacctattgg tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc
    58981 atgattttat cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg
    59041 cccgcattct ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata
    59101 cagaggatac attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt
    59161 caaatgacaa ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat
    59221 ggacgagccc tggagatttt gctgatatcc gcggtaaata tacgcctgat tggtatccag
    59281 ataatcttaa tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata
    59341 tgaatggaga acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt
    59401 ggcatttgcg aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg
    59461 gtaaaggatt cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt
    59521 tttcttttct attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg
    59581 ctttattgtt atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt
    59641 gtcttgttcc accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct
    59701 agataatcat caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt
    59761 ttcccatctt caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat
    59821 gcttgaagca attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct
    59881 ccaccatcac caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct
    59941 aatacggctc ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct
    60001 ttgatatttc ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact
    60061 tgtgcatcaa cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca
    60121 agagcagctt ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata
    60181 aattgatagt tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa
    60241 cggcctgcta aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac
    60301 ggatattttt tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga
    60361 tcaattgttg gtttgacacc taattcattg ataatttctg tctgcaggct catttattct
    60421 cctcctatgc ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca
    60481 ttttatggtt ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc
    60541 gtttatactc ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta
    60601 gtgcaggtaa ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag
    60661 ctgtaaagtc tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag
    60721 cttcttcttc tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat
    60781 caaaatttcg agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat
    60841 tgcttgataa cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct
    60901 tgaatactgc accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc
    60961 atacatcaat tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat
    61021 tcgaagcata gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct
    61081 tagatatata ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac
    61141 ctaattcttt cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt
    61201 cgactaagaa cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat
    61261 ttccataaga ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct
    61321 tcccagcacg tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc
    61381 agactgctgc atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc
    61441 cgatgacaga tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat
    61501 tcaacagtgc tgtttctact aattggcagt gcgctaaagg cccttccact tggattaatg
    61561 gttcatttgc aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa
    61621 aattttgtaa atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa
    61681 tatcggaatc attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa
    61741 aaaccgcgta tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta
    61801 aatcagctcg acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat
    61861 gcagcgtcaa actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag
    61921 ttttcatttc ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt
    61981 tctgaaaaag cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa
    62041 ctatcctttc ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta
    62101 tccgagacac gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt
    62161 tcctactaca atcgtagccg ttaagatacc gatgattgaa caaatataac caaatgtctc
    62221 tacaacataa tcaatggcaa aagtttttga agcaataata cgtctttcct tttttgcttc
    62281 ttatccccaa agaaacggat ataacttatt atgtattgaa tttttttgga aaggcgaatt
    62341 atgacaagat ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa
    62401 agatacaaag taagaaagca gctgtcaata gaatggaaac aaaaatggcg agacatcaat
    62461 cagtctcgcc ttgtttttta ttgtatacat tagatatgga gcaggttacc ataaagatga
    62521 ttattagtcg ttattaaata gaatccttcg tatttttttg ttctatcact acaatatctt
    62581 tatttggacg tttgaattgt attccggcac gttctagctc ctgtttcact cctacatgga
    62641 ctttgacatc ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc
    62701 ttgtgatttt tttcttcgta ttgatcatca cgacttgctt tttatcatat gtgtctacca
    62761 atagcagttg tgttcgatct aacgaaaata atttgtaata attaatagcc gtattcccaa
    62821 aaaagaattc cactacttta ggataaaatg tcttaatatt taaattacta ttcacaggtg
    62881 tttcgattag cttcatctca ttcctctttt ctatatagtc gatttagatc caatcttaag
    62941 ttatgttacc atctttccta gcattaagga atcggttaca attgaacctt taaagacaaa
    63001 aggatttacc ttttatcact taatagtata acataatttc gtgagaaaaa aatacaattt
    63061 tttcttgacc atctcgttac gtaaaggatt atgcttacat ttgtcaggag gaaaaataat
    63121 ggagtacaca ataaagaaaa tgtcagagat atcaggagtc agtccgagaa ccttgcgctt
    63181 ttatgatgag attggactgc taaaaccagc taggatcaat tcttcaggtt atcggattta
    63241 tggcaaaaaa gaagtcgatc gtctgcaaca catcttattt tatcgaacaa tggcattcaa
    63301 gttggatgat atccaagagg tcttagacaa tccatctttt gaccaccaga aagctttaat
    63361 caagcatcag caaatgttgc tggaaaaacg ggcacaaatc gataccctct tgacaactgt
    63421 ccaacagaca cttgatatgt acgaaggagg aagaaaaatg agtgacaaag aaaaatttga
    63481 agggtttaaa caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag agattcgaca
    63541 aaaatacgga aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata tggataaaga
    63601 aacgtatgaa gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca ctctttactt
    63661 aaaagaacca gaagatgaaa gcttagcaga ccgtatcttc caagctcata agaagtggtt
    63721 ctcttacagc tggcctgaat atcagccaaa agcgcataaa ggactaggaa tgatgtacga
    63781 agccgatgaa cggttcactg catattatga cgagcgaagt ggtcatggtg cagcaaaagc
    63841 tttaaatgaa attatccaaa aaaaggcgta atcttttaca tgatccaaca aaaaagtaca
    63901 cgtaaaatga cctaataaca aaacagcaga cacccaagtt agatttttat gctaactttt
    63961 ggtgtctgct ccattctttc acaagttttt tcatcctact ttaaatatat tcatcgttga
    64021 ataaacgtat aaggtatttt cactttatcc tttttttctg taagaaacaa ttgaaatgcc
    64081 atttctccgc aagcttccag atggtggtcg atggttggga aaccaagaag ctcgctgatc
    64141 agcaaattat cgcgtccaat caccactggt gtcttatctt gcagatagct ttgcaagatg
    64201 cctgctgcaa ccatatccga atttgtaaag acaccatcaa ctccaagctc tttaaaaaag
    64261 aagcctgctt gttttccttt ctctgcatct atacaatccc aaaaaacaag tgattcatca
    64321 aaagatggaa aaagttgatg acatagctgc aaagtgattt ttgagttata acttagcctg
    64381 ccacttcgcc ccaacgtgat gcccaattgc tttatacctt gctgcttcaa ataagtaaga
    64441 ccttctgtaa ttgattgttc tcgatcaata aagacacagc ttgcctgttt tcccttgatt
    64501 tcttcacaaa aaactagagg tccatatttt tgatatgcca ataaatcatc tagtgtattg
    64561 gcacgagacg taatgatcaa tccatcgaaa gctttagctg caaattcttc aagatacctt
    64621 cgctcttttt ctttatgata gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac
    64681 gctgcttcaa tgatcccact gatcaactga tcaaaatacg gatgattggt ataaggcaga
    64741 atcactccaa tgcttttcgt ttttccataa cttaactgtc tggcatttgc attagggata
    64801 taatcgactt ctttcatgac cgcaagtatt tctttccttt tttcctcatc tacatatgga
    64861 tgtccattga ttacacgtga aatagtagaa actgaataac ctgtgagctt tgcgatatct
    64921 cgaatgtttg ccactgctgc ttcgccacct atacaaaaag attttcttgt aaatagtcta
    64981 ccacagctgg accgcattca aaaaactgat acatttttcc ttcatgcgaa aaactgatcc
    65041 gctgcccgcc ccaatatata tcttttgtca aattttcgat ttcattcatt ggtatttctt
    65101 ttactatatt cctattcaac ccttgaaaac tgtacaacgt taatacggat cccttgaact
    65161 cgacaaacgc ataaaaataa ttgttcgttg tgccatctgt tatctctaca taagtattca
    65221 ttgcaagccc tccttttgat actttgagta tagtgtatga aacgcgtctc atagcaagga
    65281 gtttgttttg ccaaagaaaa gttttattcc tctttcatta ttaccaaagt ctcttatttt
    65341 aaattgaata cattataatg atactgtaaa tatatcttta ttattagtga atg
//
LOCUS       DACDCJ010000004        64069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDCJ010000004 DACDCJ010000000
VERSION     DACDCJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.004"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(419..922)
                     /locus_tag="IV038_000257"
     CDS             complement(419..922)
                     /locus_tag="IV038_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8340508.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1281..2306
                     /locus_tag="IV038_000258"
     CDS             1281..2306
                     /locus_tag="IV038_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8340509.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2629..3507
                     /locus_tag="IV038_000259"
     CDS             2629..3507
                     /locus_tag="IV038_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340510.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3983..4099
                     /locus_tag="IV038_000260"
     CDS             3983..4099
                     /locus_tag="IV038_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8340511.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5027..5365
                     /locus_tag="IV038_000261"
     CDS             5027..5365
                     /locus_tag="IV038_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340512.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5459..5689
                     /locus_tag="IV038_000262"
     CDS             5459..5689
                     /locus_tag="IV038_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340513.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6089..6460
                     /locus_tag="IV038_000263"
     CDS             6089..6460
                     /locus_tag="IV038_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP8340514.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6734..7426)
                     /locus_tag="IV038_000264"
     CDS             complement(6734..7426)
                     /locus_tag="IV038_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8340515.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7429..8214)
                     /locus_tag="IV038_000265"
     CDS             complement(7429..8214)
                     /locus_tag="IV038_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8340516.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8451..9851
                     /gene="lacG"
                     /locus_tag="IV038_000266"
     CDS             8451..9851
                     /gene="lacG"
                     /locus_tag="IV038_000266"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8340517.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9876..10193
                     /locus_tag="IV038_000267"
     CDS             9876..10193
                     /locus_tag="IV038_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8340518.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10206..11879
                     /locus_tag="IV038_000268"
     CDS             10206..11879
                     /locus_tag="IV038_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8340519.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11893..13524
                     /locus_tag="IV038_000269"
     CDS             11893..13524
                     /locus_tag="IV038_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP8340520.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14015..15580)
                     /gene="guaA"
                     /locus_tag="IV038_000270"
     CDS             complement(14015..15580)
                     /gene="guaA"
                     /locus_tag="IV038_000270"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP8340521.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15639..16562)
                     /locus_tag="IV038_000271"
     CDS             complement(15639..16562)
                     /locus_tag="IV038_000271"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP8340522.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16758..17363
                     /locus_tag="IV038_000272"
     CDS             16758..17363
                     /locus_tag="IV038_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8340523.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17671..18687
                     /locus_tag="IV038_000273"
     CDS             17671..18687
                     /locus_tag="IV038_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8340524.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18828..20129
                     /locus_tag="IV038_000274"
     CDS             18828..20129
                     /locus_tag="IV038_000274"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP8340525.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20175..20837
                     /gene="deoC"
                     /locus_tag="IV038_000275"
     CDS             20175..20837
                     /gene="deoC"
                     /locus_tag="IV038_000275"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP8340526.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20858..21256
                     /locus_tag="IV038_000276"
     CDS             20858..21256
                     /locus_tag="IV038_000276"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP8340527.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21322..22404
                     /locus_tag="IV038_000277"
     CDS             21322..22404
                     /locus_tag="IV038_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8340528.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22616..24178
                     /locus_tag="IV038_000278"
     CDS             22616..24178
                     /locus_tag="IV038_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340529.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24171..25304
                     /locus_tag="IV038_000279"
     CDS             24171..25304
                     /locus_tag="IV038_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340530.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25304..26260
                     /locus_tag="IV038_000280"
     CDS             25304..26260
                     /locus_tag="IV038_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340531.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26359..27525
                     /gene="deoB"
                     /locus_tag="IV038_000281"
     CDS             26359..27525
                     /gene="deoB"
                     /locus_tag="IV038_000281"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP8340532.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27549..28367
                     /locus_tag="IV038_000282"
     CDS             27549..28367
                     /locus_tag="IV038_000282"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8340533.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28384..29091
                     /gene="deoD"
                     /locus_tag="IV038_000283"
     CDS             28384..29091
                     /gene="deoD"
                     /locus_tag="IV038_000283"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP8340534.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29389..30075)
                     /gene="gpmA"
                     /locus_tag="IV038_000284"
     CDS             complement(29389..30075)
                     /gene="gpmA"
                     /locus_tag="IV038_000284"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8340535.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30247..30828)
                     /locus_tag="IV038_000285"
     CDS             complement(30247..30828)
                     /locus_tag="IV038_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8340536.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30961..31644)
                     /gene="rpiA"
                     /locus_tag="IV038_000286"
     CDS             complement(30961..31644)
                     /gene="rpiA"
                     /locus_tag="IV038_000286"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP8340537.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31963..32376
                     /gene="rpsL"
                     /locus_tag="IV038_000287"
     CDS             31963..32376
                     /gene="rpsL"
                     /locus_tag="IV038_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP8340538.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32460..32930
                     /gene="rpsG"
                     /locus_tag="IV038_000288"
     CDS             32460..32930
                     /gene="rpsG"
                     /locus_tag="IV038_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP8340539.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33024..35111
                     /gene="fusA"
                     /locus_tag="IV038_000289"
     CDS             33024..35111
                     /gene="fusA"
                     /locus_tag="IV038_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP8340540.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35283..36470
                     /gene="tuf"
                     /locus_tag="IV038_000290"
     CDS             35283..36470
                     /gene="tuf"
                     /locus_tag="IV038_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8340541.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36876..37184
                     /gene="rpsJ"
                     /locus_tag="IV038_000291"
     CDS             36876..37184
                     /gene="rpsJ"
                     /locus_tag="IV038_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP8340542.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37215..37844
                     /gene="rplC"
                     /locus_tag="IV038_000292"
     CDS             37215..37844
                     /gene="rplC"
                     /locus_tag="IV038_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP8340543.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37872..38495
                     /gene="rplD"
                     /locus_tag="IV038_000293"
     CDS             37872..38495
                     /gene="rplD"
                     /locus_tag="IV038_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP8340544.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38495..38785
                     /gene="rplW"
                     /locus_tag="IV038_000294"
     CDS             38495..38785
                     /gene="rplW"
                     /locus_tag="IV038_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP8340545.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38824..39657
                     /gene="rplB"
                     /locus_tag="IV038_000295"
     CDS             38824..39657
                     /gene="rplB"
                     /locus_tag="IV038_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP8340546.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39700..39978
                     /gene="rpsS"
                     /locus_tag="IV038_000296"
     CDS             39700..39978
                     /gene="rpsS"
                     /locus_tag="IV038_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP8340547.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40001..40348
                     /gene="rplV"
                     /locus_tag="IV038_000297"
     CDS             40001..40348
                     /gene="rplV"
                     /locus_tag="IV038_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP8340548.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40362..41018
                     /gene="rpsC"
                     /locus_tag="IV038_000298"
     CDS             40362..41018
                     /gene="rpsC"
                     /locus_tag="IV038_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP8340549.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41021..41455
                     /gene="rplP"
                     /locus_tag="IV038_000299"
     CDS             41021..41455
                     /gene="rplP"
                     /locus_tag="IV038_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP8340550.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41445..41633
                     /gene="rpmC"
                     /locus_tag="IV038_000300"
     CDS             41445..41633
                     /gene="rpmC"
                     /locus_tag="IV038_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP8340551.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41658..41924
                     /gene="rpsQ"
                     /locus_tag="IV038_000301"
     CDS             41658..41924
                     /gene="rpsQ"
                     /locus_tag="IV038_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP8340552.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            41982..42350
                     /gene="rplN"
                     /locus_tag="IV038_000302"
     CDS             41982..42350
                     /gene="rplN"
                     /locus_tag="IV038_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP8340553.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42387..42695
                     /gene="rplX"
                     /locus_tag="IV038_000303"
     CDS             42387..42695
                     /gene="rplX"
                     /locus_tag="IV038_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP8340554.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42723..43262
                     /gene="rplE"
                     /locus_tag="IV038_000304"
     CDS             42723..43262
                     /gene="rplE"
                     /locus_tag="IV038_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP8340555.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43281..43466
                     /locus_tag="IV038_000305"
     CDS             43281..43466
                     /locus_tag="IV038_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP8340556.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43502..43900
                     /gene="rpsH"
                     /locus_tag="IV038_000306"
     CDS             43502..43900
                     /gene="rpsH"
                     /locus_tag="IV038_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP8340557.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43932..44468
                     /gene="rplF"
                     /locus_tag="IV038_000307"
     CDS             43932..44468
                     /gene="rplF"
                     /locus_tag="IV038_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP8340558.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44629..44985
                     /gene="rplR"
                     /locus_tag="IV038_000308"
     CDS             44629..44985
                     /gene="rplR"
                     /locus_tag="IV038_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP8340559.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45006..45506
                     /gene="rpsE"
                     /locus_tag="IV038_000309"
     CDS             45006..45506
                     /gene="rpsE"
                     /locus_tag="IV038_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP8340560.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45521..45700
                     /gene="rpmD"
                     /locus_tag="IV038_000310"
     CDS             45521..45700
                     /gene="rpmD"
                     /locus_tag="IV038_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP8340561.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45744..46184
                     /gene="rplO"
                     /locus_tag="IV038_000311"
     CDS             45744..46184
                     /gene="rplO"
                     /locus_tag="IV038_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP8340562.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46184..47479
                     /gene="secY"
                     /locus_tag="IV038_000312"
     CDS             46184..47479
                     /gene="secY"
                     /locus_tag="IV038_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP8340563.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47541..48188
                     /locus_tag="IV038_000313"
     CDS             47541..48188
                     /locus_tag="IV038_000313"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP8340564.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48379..48597
                     /gene="infA"
                     /locus_tag="IV038_000314"
     CDS             48379..48597
                     /gene="infA"
                     /locus_tag="IV038_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP8340565.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48630..48746
                     /gene="rpmJ"
                     /locus_tag="IV038_000315"
     CDS             48630..48746
                     /gene="rpmJ"
                     /locus_tag="IV038_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP8340566.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48764..49129
                     /gene="rpsM"
                     /locus_tag="IV038_000316"
     CDS             48764..49129
                     /gene="rpsM"
                     /locus_tag="IV038_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP8340567.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49157..49546
                     /gene="rpsK"
                     /locus_tag="IV038_000317"
     CDS             49157..49546
                     /gene="rpsK"
                     /locus_tag="IV038_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP8340568.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49626..50564
                     /locus_tag="IV038_000318"
     CDS             49626..50564
                     /locus_tag="IV038_000318"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP8340569.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50592..50972
                     /gene="rplQ"
                     /locus_tag="IV038_000319"
     CDS             50592..50972
                     /gene="rplQ"
                     /locus_tag="IV038_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP8340570.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51244..52083
                     /locus_tag="IV038_000320"
     CDS             51244..52083
                     /locus_tag="IV038_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8340571.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52059..52928
                     /locus_tag="IV038_000321"
     CDS             52059..52928
                     /locus_tag="IV038_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8340572.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52918..53715
                     /locus_tag="IV038_000322"
     CDS             52918..53715
                     /locus_tag="IV038_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8340573.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53918..54664
                     /gene="truA"
                     /locus_tag="IV038_000323"
     CDS             53918..54664
                     /gene="truA"
                     /locus_tag="IV038_000323"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8340574.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(54997..57666)
                     /locus_tag="IV038_000324"
     CDS             complement(54997..57666)
                     /locus_tag="IV038_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8340575.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(57904..58092)
                     /locus_tag="IV038_000325"
     CDS             complement(57904..58092)
                     /locus_tag="IV038_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP8340576.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            58362..58724
                     /locus_tag="IV038_000326"
     CDS             58362..58724
                     /locus_tag="IV038_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8340577.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            58776..60458
                     /locus_tag="IV038_000327"
     CDS             58776..60458
                     /locus_tag="IV038_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP8340578.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            60575..62611
                     /gene="recG"
                     /locus_tag="IV038_000328"
     CDS             60575..62611
                     /gene="recG"
                     /locus_tag="IV038_000328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP8340579.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            62660..63661
                     /gene="plsX"
                     /locus_tag="IV038_000329"
     CDS             62660..63661
                     /gene="plsX"
                     /locus_tag="IV038_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP8340580.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            63783..64025
                     /gene="acpP"
                     /locus_tag="IV038_000330"
     CDS             63783..64025
                     /gene="acpP"
                     /locus_tag="IV038_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8340581.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        20561 a        11485 c        13800 g        18223 t
ORIGIN      
        1 tttcctttga aatgccttca aaaagtagga tcaggcagaa tatactggca gaatcaagga
       61 gaagggagcc taaataagga gaaaaaccat agaaaatata aagtaattac ttataaaaca
      121 agctttttat agaaaaataa tatacctgag tagggtataa attaagacgg aattcctttc
      181 tagattgaga tattgactag gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa
      241 ccctttttgt taaataaggg gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct
      301 gcaataatat agtagattag agatgaaaat agattttttt aaaccaatga ctgaataaaa
      361 agaccgtcat agatacacat tggaatgtgt ctattacggt ctttttttac gaacgatttt
      421 attgaagacg gacagttcgg gtcaaaaata aaaaagctac gatcagaaat aagaataaaa
      481 tgtatcccaa aataatggct agacggtcta acttatttgt cgatagatgt ttgtttagaa
      541 gttgataggc ccataaacct atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt
      601 ccgctacgcc aataccgaat aggaattgaa tcaattctgc caagaagctt acaaggaaaa
      661 taatccctaa ttttttagaa aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa
      721 aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag
      781 aaaaagtaga aaaagggacg agattgacat tgctgtaaga ataagtgaga aattgtggaa
      841 gattaaatga tagcttgaat aaaatcaacc ataatagaaa gaataaataa atccctaata
      901 aaaattttga tacataccgc atagacatct cctccattta aaaagtatac catcgaaaac
      961 cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat
     1021 tagaagcttt tttttgttgg gaatccggtt tctttaacaa agctaagata gcgagttgct
     1081 tggaaaagtt gctatctttt ttctgtcaaa acgactcgga atgttcgttt tttgttctaa
     1141 tttctccaag ttgtttactt tttgatacta agtttatgaa aaatcaagaa atagttgtta
     1201 tactaggtaa tgctgacaaa cgggattggc atttcatctt ggttcagctt tttttccaat
     1261 tactaggaag gagggataga ttgttcaaga cttattttcg tttcaataaa atacaagata
     1321 atccttttcg tatcatgaat ctgatgctac ttatctttat tgtattatgg ttaggttaca
     1381 taaatcatga ttttttgatt tcaagttttt catcattagg gatctttact tttttctatt
     1441 atcaaaacat accgatgaaa tatttgctga aacgtatgtt tctgatcggt tgcggactgt
     1501 tggttgcttt cgttttgggg atcttatcga cttatgtttt atggcttgaa ccatttgccg
     1561 tagctttggt tgctttttcc agcagattcg tgttgcgatt attccatatt tctaaaccag
     1621 gtggcttgtt ttttgcgatg ctttctgcca tgggaacgag catgcagctg ccaatcgctc
     1681 aattaccgat cgtctcactt tattttttta tgggtgtcgt ttttgccctg attgcggcag
     1741 tgattacaaa attactggat tcacggcctg agcagacaat tgaaaaagca actttaaaag
     1801 aaagatttca cgaagagcct ttagtgatca ttgattctgt tttctacagc gctgccttat
     1861 ttctttccgt ttatgtcagt cacggcttga atctgcataa tccttattgg ttgaccttgt
     1921 catgtgcatc gattttgcta gcagaaaact tggatgcgat gaagcatcga caagtccaat
     1981 atctgattgg atcgatgggt gggttatgtg tgtcggcatt cttgtcattt gtgccgttta
     2041 cgcagttgca gaccattttt ctgatcactt ttctatatgg aatcgcacaa tttctagttg
     2101 ctagaaatta tgcagttgct aatatttttc ttaatccgat ggcgttgatg ctatctacac
     2161 ttattcgtgg agcatacttg atttctttga tcgaatatcg ttttttaggg atcgtcatag
     2221 gaagtttcat cggtttaggt gttgcgtggg taatgacggt tggtttgcaa cattatttaa
     2281 cggttgttcg tcaaaaatta gaatagaagg acaaatatat gtgccaaaag aggttatgac
     2341 aagatttttg tcacaacctc taattcaagg tgttcaaaag ccagattctc agcattaagt
     2401 caaattcaca caaaaatgtg gaaaggaact ttcaaaaaaa ttttatattt attctatcaa
     2461 aaaattttgt ttatgatggt aactgaataa actttcctga gatggtcaat gctttccttg
     2521 aaagtcttac agatattctt tcttcataga aagtttaagt tttatagtta gtgtacaata
     2581 aaagtatgtt tataccatat ggtgtccgga taatcagtca tttaaacaat gaggttcaaa
     2641 gatatgaaaa ggggaaattt gatgaagaaa ataagcaatg aaatccaaat tctggcagaa
     2701 actttaactc ttcccaaagt cgttatagag caaatgattg tacaagcaaa aaacatcctt
     2761 gatgaggtgt ttgagcaata tagtcatgaa ctaatggatc ctaataaagc aaaggcgact
     2821 gcaaagaaaa tagacaaagt ctatgaagag gaaccttttg cagcattagc tatttatcta
     2881 tatgcagctt ctcaaagttg gaaaaaaatt tatcaaccat tgaagattcc aagaactatc
     2941 tatttggcaa caatgaatgc ttttactaga tttattcatg aacatttcca agtaacagga
     3001 agctatcgct ttgaccgtgg tttttggatt tggcattaca ctagtggctt gatctttcgg
     3061 attggtgaat tggagtacga aatgacatat tttccgaaag ggtacacgga tctaagatta
     3121 gaaggaaagc agtgcctttc tcttcatatt ccttccgatg cagatttatc tcagaataag
     3181 ataagagata gttacgaaag tgccattcgt ttttttgaac tttattttcc tgattaccat
     3241 taccaagtga tgtacacaga tacttggtta ctatcaccca atcttactaa atggttgaaa
     3301 aaagagtcaa aaatctgtct ttttgcagcc gattatcgat tattatcagt tgatgaacag
     3361 gacgatagtg gagtcccttg gatctttgga cgagtagatg cccaaataca tgattatcca
     3421 gaaagtacga gtttacagcg acaagcaaaa gagcaattgc ttgcaggcga gcacattggc
     3481 tctggcttag gtattcttac ttcataagtt tagtacttcg aaatagttta aaagagcgtg
     3541 agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag
     3601 aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa
     3661 tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctttttta gtgttctatc
     3721 gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa agttaacgga
     3781 caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc cagagatagg
     3841 agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt aacttttgat
     3901 accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg actacaatca
     3961 aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat accgccgctt
     4021 gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg
     4081 gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt
     4141 ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg ctgttgattt
     4201 tccattttta acatatgata aattggtaga atctttcttt tcaaattgat accattccct
     4261 aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata aaagtattat
     4321 ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta ttgatcactt
     4381 taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg aaacgtaact
     4441 acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag cgagcaatac
     4501 ggttgcaaat aaccattatc gtttaataag cagttacgaa agctcccaat atctctaacg
     4561 ttcagctcac tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg
     4621 tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag
     4681 ttactattgc tagaataaca tcaaggaata ataagttttg gttttcagat aatagtcgat
     4741 aagttgtgta ttggaatttt agatacagta ataaagtatt tttcatgaaa ttttgtgaga
     4801 atcactatta caagtatctt taataaaatt aatggttaag gaggaactta aagtagaatg
     4861 taaatgaata gttgtagtca gtcgattgtg atactagact caaagttgca ggaataagtt
     4921 tttcaatttg tgattggaag ataaaaaatg aaaaagaagg gctttttttt atactttgtt
     4981 gattgtagtg tttattttcc ctctgtttag ccaagtaaca ttttctatga ctcaaccagt
     5041 ttcaagtgag gaacatgata acctttttcg tgctacagaa gagtttaaga aatatcagga
     5101 aaatattgtt ccggagactc cattagctag tgagccaatt actgatattg atggaaacca
     5161 aataggatgg tatatacagt acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa
     5221 tacagtaggc ttagagctaa caccgattga aaaggtgagt tcattgttaa cgtatattta
     5281 tactgatgaa agtgacgaga ctgagatcat tttaacggat tactcaaaaa ttgctactga
     5341 taaaaagtat atattgtgga tgtagacact agtgaaatac tttatgatat agaaattgat
     5401 gaaaatttac tagatgtagc acaagaaatc gatcatcaaa agcaggaagc tattcgatat
     5461 gctggtagaa aagggaaaag aaaatattta ttacgtgaat gtagcaaaag ttagagaaga
     5521 tgaaaatgag tggaaagaat tcaagagtcg ctactcaatt aactcgactc cgacatttac
     5581 agtttacaga gaaggatcga tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc
     5641 tttagctgaa gtagaagagt ttttggatta tgttagtatg caacagtaag ggagttctta
     5701 ttatgaaaaa ttttgacaca tattatgtta ttgttttttc gatatctttg ctgttaatat
     5761 tactaacttt aatgcagtat aaagagtatc cagagtattt tattttaact tttgtatatt
     5821 tagtaagtag ctacacactt ctatataaat tcagaagttc ttctagagtt tctaaagttt
     5881 gtttcttaat tagcttattg attctataat atttttgatt tatataggaa attaaaaata
     5941 ttatagaatc aacactcaga gaacgggttg gtttttaata gatttaataa gtactgataa
     6001 tgaaatttct cctggttggg aaaacgaagt tggttaccct gtagaagaat tttttcaaga
     6061 ttactttgcg gagtatgccg ataggataat gagagattac gtaacagata aaactcaaga
     6121 agtcatcaat caatcccagt cattaaatga attatctgat aaggcgaaag aagaagcagt
     6181 tgccattatt tcttcaggga ttagtgacta tattgatatg agtggtgaaa tcaattatgg
     6241 cgataaagac aaaattgtac gcatgataaa agcttttatc aaaacttttg atgtgaaggc
     6301 agatcttaag tttaatgata cttacctagt aggaaaatta attaatatcc ttgggacaga
     6361 gaaagatacg gaaacgttta tttcctcttt gtctatgttg tttacagata agcatttttc
     6421 aactcagctt ggtggagagg aactagtgga aatcttttaa ccattacgcc cagcattaat
     6481 aaaagtatat caaaaaagta ctcctgatga tttttaagat tggtttgaga aataatgtga
     6541 aaacactatt tttaatgtta attatagata gagatgaacg cgtcatatgg ttaaaagaat
     6601 gaaaaagtca aagatacact gctatatttc actaaggata tagcagtgta tctttcatgt
     6661 cgtttcactg gcagtttttt ataaaaggag gtgtgcattt cagttgcttt tctttggtag
     6721 ttagttgaat cgtttaagaa ttaacggatg tggcagacag gtgctctgtc caaaaaccag
     6781 taaaatcttt gatggttcca tctttattta gctgtgcctg ttcaaatact gttttgtttt
     6841 taccgaatgg atcaattgca ataattgttc cgatccccgc acgtttggca gcaagtaatc
     6901 ctgagtaagc atcttcgata acaagacaat cagccggagc taagctcagt ttgtctgccg
     6961 ctttaagaaa aatatccggt tgcggtttcc cagggaaaga gccgtcgtca tagacaatct
     7021 tgtcccagtc aaaccagcgg gctaaatcga aaatatcaaa ataaaaagca acattctctt
     7081 tgacagtagc cgtggcaatc gttaatggaa tctgttggtc agtcagctgg tcaagagtcg
     7141 cttcaagccc ttttgtcaac tgaagctcgt tttcattttg caagcaaagc atacgataat
     7201 agctttcttt ttcttctgac aatacggcga tctcagaggc ggtcaaatgc tctgaaatga
     7261 aatgtttcaa aatctccgta ttcgtccgac catgaatatt ttttaaaata tcctcgtctg
     7321 ataaaccatc ttttgtatga tcatgtatca tatgcagcca agcagcttca tgcagatgag
     7381 agtccaaaaa cattgttccg ttgaaatcaa atataatccc tttcatagtt acgctcctat
     7441 agtggtattt tcggttgaat cagcaatttg cagatcgaca tattgtgcat agtgctcttg
     7501 aacctcccgc gagatctggc gatccgtaat gagcagatcg aaatcatgca aattataaaa
     7561 cacataaaaa tcatctcgat tgaatttttt attatcaacg agtagatatt tcttacggga
     7621 attatttaga gcgatctgct gtgcttctcc ttcttcggta ctgtaagtag agatcgcttc
     7681 gttgtgaacg ccatttgaac tgataaatgc ttttgtaaac tttagtcttc ttaaattgtc
     7741 gttggtgatc ggtccgacaa atgcgccggt atttttccgg aaatcaccgc caatcagtag
     7801 aatttctggt ccatctgctt gagcaagaat atcaaaaact ggataattat ttgtaatgat
     7861 ccgaattttc ttgttcagta aatgcgtcgc caatagttcg attgttgtac ctggtcctaa
     7921 gaaaatcgtt tctccctcgt gaatcaagga ggctgctgtg gcagcaattt cttttttttc
     7981 ttgaatctgc agagtggctt tttcagtgtg agaaagttct tgatctaaag tgtaggaaag
     8041 actttgagcc cctccatgga cacgcagcag cttgcccgat ttttctaatt catctaaatc
     8101 tctgcgaacg gtcatatcag aaacatctaa ttgattgatg atttcattaa cggtaaggat
     8161 tcctttttta ttgaccattt ctacgatagt aagcaatctt tcttttttca acattgtctt
     8221 cacctctaat ttaaggataa tctatcatat aaacggacaa cagtcaatct aattcgaaca
     8281 aaagtaaaca tttagggtgg tttttatgtt tgttacaata gattttattg ctgttttctg
     8341 gtttttatga ttatttgtgt ttgtttttgt tgacaaatgt tttggatgcg tttacaatgc
     8401 aatcagttag aaagagcgaa aactgattat agatttgagg agagatgtac atgcttaaat
     8461 taccagaaga ttttattttt ggcggagcca cagcagccta tcaggttgaa ggagccacta
     8521 aggaaggtgg caaaggcgct gttgcttggg atgacttttt agaggaacaa gggcgattca
     8581 gtcccgatcc ggcaagtgat ttttatcatc aatatgcaaa ggacattgaa ttgtgtgaac
     8641 gttttggtgt aaatggtcta agattatcca ttgcttggag ccgcattttt cctgatggag
     8701 ctgggaaacc aaatccagaa ggaatcgctt tttatcatcg tgtgttcgaa gaatgtaaga
     8761 aaagaaacgt aacgccgttt gtgacgttgc atcattttga tacccctaaa cgtttgtttg
     8821 accatggaga ttttttgaat cgagaaacaa tagaagcatt tgtttcctat gccatttttt
     8881 gttttcatga attcaaagaa gtcaaggtgt ggagtacctt taatgaaatc tatccagttg
     8941 caaccaatca atatttgtta ggtgttttcc caccaggaat caaatatgat tttacaaaga
     9001 tcgtcgcctg tcttcataat atgatggttg ctcatgcccg tgtagtgaac tatttcaaag
     9061 aaaacgaact tccgggtgaa attggtgtag ttcactcatt agagacgaaa tatgcggcaa
     9121 cagacgcgcc ggaagataag catgctgcct ttttagacga tgcattgtcg atccgtttct
     9181 tattagatgc tacctattta ggctattatt caactgaaac cttgactgct cttgatgaga
     9241 tatgtgaagc aaatcaggcc agctatcatt ttcctgaaga agatttcgtt gaattgaaaa
     9301 aagcatccac gcgtaatgac tacttaggga tcaatcacta ccaatgccat tttgtcaaag
     9361 cttacgatgg agaaaatgca atccatcata acggtacggg tgaaaaaggg acctctgttt
     9421 ataaagtgaa gggaatcgga gaacgtattt ataaagaagg aattcctcga actgattggg
     9481 actggctgat ttatccagaa ggtctatatg acttgttgct gcgaattaaa agcgattacc
     9541 cacattacaa caaaatctat atcactgaga acgggatggg gtataaagat cagtttgaag
     9601 atggcattat tatggatcag ccgagaatcg actatttgag agtctatttg gaatccttaa
     9661 gcaaggcgat aacagccggg gtaaatgtga aggggtattt tttgtggtcg cttatggatc
     9721 ttttctcatg gacaaatggg tataacaaaa ggtatggctt gttttatgtt gattttgaaa
     9781 cccagaagcg ttacccaaaa gaatcagctt actggtacaa gttggtcagt gaaaccaaaa
     9841 cgattattta ataagtgata atggaggaag aaaagatgaa taaagaggaa ttacaaatgc
     9901 ttggttttga gatcgttgca tattcaggcg acgctcgcag tacattattg aaattattga
     9961 aagaagtaag acaaggaaac tttgaacatg ttgattcggc gttgaaagaa gccgacgaaa
    10021 atttaaccct tgcacataat tcgcaaacaa aaattttagc ggaagaagca tctgggaaag
    10081 agatggagat gggattcatt tttatccatg ggcaggatca tttaatgacc actttattgc
    10141 tgagagatct gattcaagac tttattgtac tgtacagaca gaatcaagga taggagaaga
    10201 acgctatgga tggaatcatt aaacaaatcg aaaaagggaa accatttttt gaaaaaatct
    10261 cacgtaatag ttatttagga gccgttcgtg acggattttt aacagcgatg ccagctattt
    10321 tattttcaag cattttcatt ttgattgctg cggttcctga gatttttggt taccaatggc
    10381 ctgaatctgt ctccacatgg ctatggaaag tctataatta ttcgatgggt gttgtagggt
    10441 tactcgtttc agcaaccact gctcgctgct tggctgattc gatgaatcgt cgcttaccga
    10501 aaaacaaagt aatcaacacc acctctgtga tgctggcctc tgtggtaagt ttcctgttgt
    10561 taagcgtctc tccgattgaa ggtggatttt caacggaata tatggggaca aagggactac
    10621 tggcttcctt tgtcgctgca tttttaacag ttaatttata taagttttgt gtgctgaaag
    10681 atattaccat taaaatgcct aaagaagttc ctggaacgat ttcgcaaacc ttccgagata
    10741 tttttccttt ctctttttca gtatttgcgg ccgttttaat tgatttaggc attcgacaaa
    10801 tcttccaaat ctcatttgca gaagcaatca ttacggtgct gcagccgcta tttacagcag
    10861 ctgacgggta cttaggaatc gctattatct ggggagcgat ggcgctgttt tggtttgtcg
    10921 gtgttcatgg accgtcgatc gtggagccgg caattgcggc aattatctat gccaatgtgg
    10981 aaaccaatct gcaattgttc aattccggtg aacaagcttc taaagtactg acggtgggtt
    11041 tagggaattt tgtaggaact atgggaggaa ctggggcaac attggttgtt ccttttatct
    11101 tcctactctt tgctaagtct aagcagttga aggcagttgg gaaagcttcc tttattccag
    11161 taagtttcgc tgtcaacgaa ccattgctat ttgcggcacc catgattttg aacccttatt
    11221 tttttattcc atttttgtta acacctgttg taaacgtttg cctgttcaag ttttttgtcg
    11281 atgtattggg catgaacagc tttatgtacg ttttgccttg ggcaactcca gcaccgatcg
    11341 gtttggttct aggaacgggc atgggtatct tagcttttgt tctagcagcg gttctaatcg
    11401 tcgttgactt cctgatctat ctaccatttt gcaaagcata cgatgctgtc ttagtagaga
    11461 aagaagcaca aaaagcagag gtgatcgcaa ccgaggaagg tgcttctgcc gatgtacgtg
    11521 aggaagtacc tgtggcagcg agtcaagtta tcagcgatgc aaataaagaa atcaatgtgt
    11581 tggtactttg tgctggcgca gggacaagtg cgatgttagc taatgcttta aaaaaaggtg
    11641 catctgaatc gaaagcaccg atcagtgcaa tggcgggtgc ttatggttct cattacgaaa
    11701 tgctgcagga ttttgatatg gtggtcttag caccgcaagt caattcttac tatgatgata
    11761 tcaaagaaga caccgatcgc ttaggaatca agctggttgc gacaaaagga gcagaatata
    11821 tcggattaac gagagatcct gaaaaagctg ttcagtttat tctgaaccaa tttcaataaa
    11881 ggaggagcca taatgaaaat gggaatttgg ttgcgtaaag ctgttttatt tgcagctgtg
    11941 ttaggggctt tgggggctgt ttcaaaaact gttgaagcat ctgccagcga atttattcgc
    12001 ggagcagata tttcgatcct gtcagatatg gaaaaatctg gtgcaaaata ttatgaagac
    12061 gatatttcaa aagatgcctt agagatttta aaaaataatg gtgtcaacta tgttcgactg
    12121 cgtttgtggc aggatcctta taatagtaat ggagaaagtt atggagcagg gacaaatgat
    12181 ctggaaacga cgattgacct tgcaaaacga gcaaaaaatc tgggacttaa ggtgctgctg
    12241 gattttcact acagtgattt ttgggttgat cctgggaaac agaatcttcc taaagcatgg
    12301 caaggtctga cctttgaaga aatgaatacg gcattgtacg actatacaaa gaacgttctg
    12361 tcggaaatga agcagctgga cgtttatcct gatatggttc agatcggcaa tgaactgaat
    12421 agcggaatgc tctggcctta tggcaagtct tggggagaag gcgggggaga atttgatcga
    12481 ttggctgctt ttttgaagtc tggtatccaa gctgttcgag acactcaacc taaaacaact
    12541 ccagtaatgc tccacttagc tgatggtggc gatacaggag catttacttg gtggtttgat
    12601 gaaatcacga gtcgaggtgt gtcatttgat ttaattggag tgtcgtatta tccgtattgg
    12661 cacggttcaa tgaatgaact tcaaaccaat atgaatgcga tcagccaacg gtatggtaaa
    12721 gaagtgatcg tagttgaaac agcttatggg catacaacag cgaatgccga tactatgcca
    12781 aatgcttttg gtgaagccga agcagctgcg gggggatatg aaccaacacc gacaggacaa
    12841 gcagagtatt tgctggatct tgccgatcgg attcaggcag taccgaataa tcgcggggcg
    12901 ggcttcttct attgggaacc tctttggtac aacggaaatg tgagttgggc aacacaagca
    12961 ggtatgaatt atctcagtgt tcaatccact atgggaaacg aatgggacaa ccaagcgatg
    13021 tttgattttg ctggaaacgc cttgccaagc ttgcgtgcgt ttaaacaagc aggggcgcaa
    13081 ataaatctag ttaaaaatgc aagtttcgaa agcgatggac caacgaccag tccttccaat
    13141 tggcaaacct ggtttcaaca aggaaccgct aagggtacga tcaaaacgga agcgggcact
    13201 ttttttgggg actacaaact aactttttgg caggatcaag catacgaggc atcagcttat
    13261 caaaatgtta gtggcataag caaaggaact tacacattga gtgcctatgt aatgtctggg
    13321 ggagagtttg atactaatca gttatatatc aaaaattatg gtggagcgga actaaatact
    13381 gcggttgtta aaacaagtga atggacaaaa attacaatca aagacattcc aattacaaca
    13441 ggtgtttgtg aaattggggt ttatagcaag gcttcaggaa atgcttggac gaacgttgat
    13501 tttgtttcat tggtgaaaca atagactgcg gatcgtttcg attcaagatc ggaggttccc
    13561 ttcagccaca tcgttagcgt agaaggcagg aatcgaacct gcacctcgcc tttgccaaaa
    13621 ataaccagaa acgatttcca gtcattttta ccaaaacgag taaagcacca gacttcatct
    13681 acactttgta ttatgctaaa gagagttcaa cgatttcgta agaagcaatg aaaaaattgc
    13741 aaaaagttgt gcagataaca taaagtttgt ttaagaaagg gtaaccttag aattccgtcg
    13801 tgaatatgat ttgagcactt agatggatag aaaaaagttc tcggaaaaca gttcgttttt
    13861 cgagaacttc tttttatgat aagaatgaaa caaattaact tatattatag tctcttttgg
    13921 tagggaacca tcttattttt aatgaaaatc aagcatttta gctattgaag aatgttgatt
    13981 tatagggatt ccccctccat attatgttgg ctctttattc ccactccact gttgctggtg
    14041 gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta
    14101 cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga
    14161 ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac
    14221 cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag
    14281 cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt
    14341 cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa
    14401 cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg
    14461 tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt
    14521 gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc
    14581 cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa
    14641 catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca
    14701 acttgtctaa gaagcgatct cttgcattga ctttgatgat gttcaagccg aattttccgc
    14761 ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga
    14821 tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa
    14881 cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta
    14941 cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg
    15001 caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga
    15061 taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc
    15121 cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg
    15181 tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg
    15241 ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca
    15301 attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga
    15361 tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg
    15421 gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc
    15481 caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga
    15541 taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt
    15601 taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc
    15661 gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc
    15721 aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta
    15781 aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa
    15841 gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg
    15901 gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt
    15961 aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac
    16021 ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga
    16081 ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat
    16141 cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca
    16201 gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt
    16261 ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt
    16321 acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc
    16381 atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa
    16441 atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca
    16501 ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc
    16561 atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac
    16621 cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg
    16681 agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa
    16741 tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg
    16801 attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg
    16861 gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt
    16921 gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg
    16981 ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag
    17041 cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc
    17101 aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc
    17161 cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga
    17221 agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa
    17281 agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata
    17341 tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac
    17401 tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa
    17461 agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa
    17521 ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt
    17581 atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat
    17641 agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa
    17701 atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt
    17761 agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat
    17821 tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat
    17881 gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg
    17941 ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta
    18001 caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct
    18061 gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc
    18121 ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa
    18181 gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact
    18241 ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc
    18301 ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact
    18361 gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc
    18421 atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc
    18481 tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca
    18541 gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc
    18601 gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag
    18661 agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca
    18721 aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt
    18781 cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg
    18841 atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg
    18901 tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa
    18961 ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt
    19021 ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag
    19081 gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga
    19141 cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag
    19201 aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg
    19261 aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt
    19321 atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta
    19381 tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg
    19441 gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg
    19501 ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg
    19561 aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc
    19621 cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca
    19681 aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag
    19741 cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac
    19801 atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag
    19861 ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc
    19921 gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg
    19981 gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca
    20041 cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa
    20101 cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga
    20161 aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg
    20221 catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg
    20281 tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag
    20341 ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg
    20401 aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg
    20461 ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag
    20521 caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa
    20581 tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg
    20641 gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac
    20701 cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga
    20761 tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa
    20821 ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg
    20881 gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt
    20941 aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc
    21001 atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg
    21061 agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg
    21121 tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt
    21181 aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac
    21241 tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt
    21301 attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca
    21361 ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt
    21421 ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac
    21481 gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga
    21541 ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc
    21601 ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa
    21661 ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc
    21721 gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct
    21781 tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc
    21841 ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat
    21901 gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc
    21961 tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc
    22021 atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac
    22081 gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc
    22141 gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt
    22201 ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa
    22261 cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac
    22321 gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt
    22381 ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac
    22441 aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt
    22501 tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc
    22561 gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt
    22621 tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc
    22681 aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa
    22741 cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg
    22801 gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt
    22861 agggattggg atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa
    22921 catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa
    22981 agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg
    23041 tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc
    23101 agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt
    23161 aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa
    23221 gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat
    23281 cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag
    23341 aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat
    23401 tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga
    23461 ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt
    23521 gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga
    23581 agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga
    23641 agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca
    23701 aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga
    23761 acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc
    23821 aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga
    23881 tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt
    23941 tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt
    24001 cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat
    24061 tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg
    24121 ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc
    24181 gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct
    24241 taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc
    24301 tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt
    24361 tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc
    24421 tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag
    24481 atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga
    24541 tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa
    24601 caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc
    24661 ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc
    24721 ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag
    24781 caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa
    24841 tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta
    24901 cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag
    24961 gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg
    25021 gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct
    25081 tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg
    25141 agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc
    25201 gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag
    25261 aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt
    25321 aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact
    25381 aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt
    25441 catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat
    25501 gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca
    25561 cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct
    25621 gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga
    25681 taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat
    25741 tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt
    25801 cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga
    25861 aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt
    25921 actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg
    25981 taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt
    26041 tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag
    26101 cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca
    26161 aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg
    26221 tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg
    26281 gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa
    26341 aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga
    26401 attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac
    26461 attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc
    26521 cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa
    26581 gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc
    26641 aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa
    26701 gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac
    26761 gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct
    26821 gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc
    26881 caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt
    26941 ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg
    27001 aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct
    27061 gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa
    27121 agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc
    27181 agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc
    27241 tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa
    27301 gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc
    27361 gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg
    27421 gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt
    27481 tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga
    27541 gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata
    27601 aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag
    27661 atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga
    27721 cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag
    27781 ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag
    27841 tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg
    27901 tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg
    27961 gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga
    28021 gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt
    28081 tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg
    28141 agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag
    28201 tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag
    28261 cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca
    28321 aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat
    28381 aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg
    28441 ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac
    28501 aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt
    28561 caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg
    28621 tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat
    28681 gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat
    28741 ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag
    28801 atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc
    28861 tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa
    28921 atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg
    28981 atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct
    29041 acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat
    29101 gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt
    29161 ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt
    29221 tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg
    29281 tttgatgaat attgtgtctt gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa
    29341 aaagggactg tgacaaatcg tgtcacagtc ccttttttat agtgtttttt ataggtagta
    29401 ttttttagca actgttaagt catcatttaa ttcataaaca agtggttgac ctgttggaat
    29461 ttcaagatcc atgatgtctt cgtcagagat accttcgata tgtttagcta gggcgcgtaa
    29521 tgagttacca tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg gagcaatttc
    29581 atcttgccag aatggcaaag cgcgttctaa tgtcactttc aagttttctc caccaggaat
    29641 atcgcgtttg tctaacattg cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc
    29701 ttccattaat ggaggaagag tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc
    29761 gtatttttca gctgtttctt ttttgttcaa accttgtaat ttaccatagt gacgttcgtt
    29821 taagcgccat gatttgattt gtggtaccca aagttgatct gaatattcta agatcaagtt
    29881 acatgttttg attgcacgag ttaaaacaga agtgtaagca acatcaaatt cgattccagc
    29941 ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg ctagatttac
    30001 atcagcccaa ccagtaaatt ggttcaatgc attccattca ctaagaccgt gacgagaaaa
    30061 aactaatttt ggcatgttta gctctccttt taagataggt ttatatttct gaaattttca
    30121 gttctgtttt attgtacaat tttttggaag agatttccat cgaaaaatta gataagattc
    30181 tatgagaaaa caagtagaga atataaaaaa acgaacagtg cccacgcaac tgttcgtttt
    30241 taggtattag atgcataatt gctcgatttc tttttcaggt aaattgatat atttataaca
    30301 tgttccaact gaaacaccgc attttgaaga aataaattgg atcgtttctt ttttttcata
    30361 atagagacgt cggatttttt tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt
    30421 tcctttttta cgagcttcat ctaatccaac taatgtacgt tctttgatca atgcgcactc
    30481 catgtttgct aggttttcca ttaattggaa atagattttt cccatttcgc gcgtatcgat
    30541 tccttcatca agactaatca agtgcagccc gcgatctttg aataattgcg tgagttctgt
    30601 taattgccgc gtagatttgc ctaatcgatg aagttggcaa atgactagtg catcgccttg
    30661 ttgcatgtca gcgatcactg actccaaatg aacagcagta gaatcatttt gttccacttc
    30721 atatgtctca tggaaaattt cttgacaacc aaagtcagat aattgatcaa cctgagttgt
    30781 taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata attttcatgg tgattccttt
    30841 ctagcagtga ttttctaact ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa
    30901 gatagttttt tcgcaaattt tccgctgtct ttttttattg gatgatcaag cagtttttct
    30961 ttatttacga cggattgcgt caagaatctc aacgcctttt tctgagccga caactacttt
    31021 agaaaccatg tttaggaaaa gcccatgttc tactacccca acaagttcat ctaaataggt
    31081 agctaagctt tctggctgtt cgatgacttc tagatgcaga tcaatgatgt aatgaccacc
    31141 atctgtggtt aagacttctc cgttttcgtc tgtacgcaag acgggttgga atcttttttc
    31201 ttcaaaaata tgaagtagct gttgggaacc ataagggatc acttcaactg ggagtgggaa
    31261 cttcccaagt ttatcgacta atttagaaga atcaacgatc caaatcgttt cttttgagta
    31321 cgtagcgact attttctcga aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc
    31381 agcactgatt tcatcagcac cgtctattgt caaatcgact acggggactt catcaatact
    31441 ctttaacggt attcctaatt tttccgcttg ttcttttgtc gcatttgaag tagtaactcc
    31501 tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc tcttctacca tataataggc
    31561 ggttgaaccg gtcccgagac ctacgatcat cccgtccttt acgaatttcg cggcttcaat
    31621 gccagctaat tgttttaaat tcatctttgc gtccaccttt ttttagtttg agcatcgtgc
    31681 tttcctcact attttagcgt gatttacggt aaaaagatac cattcttttt tattccaata
    31741 aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg tgatattcta
    31801 acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat
    31861 atttgataag gatgcagaca ttgcatttat tttttatcac aaaaaaagaa ccacctggat
    31921 gtgtggaact agacgcaaat cgtgggaagg aggaaacaag gaatgcctac aattaaccaa
    31981 ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa
    32041 ggatataaca gctttaagaa aactcaaact aacgtaaact cacctcaaaa acgtggggta
    32101 gcaactcgtg tgggtacaat gacacctaaa aaaccgaact cagctttacg taaatatgca
    32161 cgtgttcgtt tgtcaaactt aattgaagtt actgcttata tcccagggat tggtcataac
    32221 ttacaagaac acagtgttgt attattacgc ggtggtcgtg taaaagactt accaggggta
    32281 cgttaccata tcgttcgcgg tgctttagat actgctggtg ttaacgaccg taaacaaagc
    32341 cgttctaaat acggaactaa aaaacctaaa gcttaatttt aattattaaa caaatgaaac
    32401 ttgcttctac tatatagaag taattactat gaacaatatt tcggaaggag gagttacgga
    32461 tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc agatcctatt tataactcaa
    32521 aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta
    32581 acattatcta taatgccttt gacttgatca aagaatctac aggtaacgat ccattagaag
    32641 tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga agttaaagca cgtcgtgttg
    32701 ggggttcaaa ctaccaagta ccagttgaag ttcgccctga acgccgcgtt actctaggtc
    32761 ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag
    32821 caaaagaaat catggatgct gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca
    32881 ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa gattctctct
    32941 gtttggtcgg cttttataag tcgacctcag atagtgtcct tatttttagc aactagatag
    33001 taaagagagg agaaacaagg agaatgacca gagaattttc tttacaaaac actcgtaata
    33061 tcggtatcat ggcccacatc gatgctggta aaacgactgc tactgaacgt atcttgtact
    33121 atactggtaa aatccataaa attggtgaaa ctcatgaagg agcttcacaa atggactgga
    33181 tggaacaaga acaagaacgt ggtattacga tcacttctgc tgctactaca gcacaatgga
    33241 agggtcaccg aatcaacatc atcgacacac caggacacgt agacttcaca gttgaagttg
    33301 aacgttcatt gcgtgtactt gatggtgctg taactgttct tgatgcacaa tcaggtgttg
    33361 aacctcaaac tgaaacagtt tggcgtcaag caacaactta tggtgtacct cgtatcgtat
    33421 ttgcaaacaa aatggacaag atcggtgctg acttcttata ttcagtatca actttacatg
    33481 accgtttgca agcaaacgct cacccaatcc aattaccaat tggtgcggaa gacgacttca
    33541 caggaattat cgaccttgta acaatgaagg ctgaaatgta tacaaacgat cttggaacag
    33601 agatcgaaga aacagaaatt cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa
    33661 aattggttga agctgtagct gaaactgatg aagaacttac tttgaaatac ttagaaggtg
    33721 aagaaatcac tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat
    33781 tctacccagt actatgtggt tcagcattta agaacaaagg ggttcaatta ctattggacg
    33841 ctgttcttga ttacttgcca tcaccgttag atatcccagc tatcaaaggg attgacccta
    33901 aaactgatga agaagtagaa cgtcctgcag acgacagcgc tcctttctca gctttagcat
    33961 ttaaagttat gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg
    34021 ttttacaatc aggttcatac gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc
    34081 gtatcctaca gatgcatgcc aactctcgtt cagaaatctc tgaagtttac gctggagata
    34141 tcgctgctgc tgttggtttg aaagacacaa ctactgggga cacactatgt gatgaaaaga
    34201 gtctagttat tcttgaatcc atggaattcc cagaaccagt tatccaagtc gctattgaac
    34261 ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt acaaaaacta tctgaagaag
    34321 atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc gctggtatgg
    34381 gtgaacttca cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta
    34441 acgtaggtgc gcctcaagta tcttaccgtg aaactttccg tgctggcact caagccgaag
    34501 gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg attgaattta
    34561 caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc
    34621 ctcgtgaata cattccagct gttgaaactg gtttgaaaga tgctatggaa aatggtgtct
    34681 tagctggcta tccattagta gatatcaaag ctaaacttta cgatggttca taccatgatg
    34741 tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt gcagcagcta
    34801 agaaagctaa tccagtaatc cttgaaccaa tcatggctgt agaagttgtt atccctgaag
    34861 attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg
    34921 aagctcgcgc tggcggccaa caagttgttc gtgcgatggt tccacttgct gaaatgtttg
    34981 gttatgctac aactcttcgt tctgctactc aaggtcgtgg tacatttaca atgacatttg
    35041 accactacga agatgtacca aaatcagtac aagaagaaat cattaagaaa aacggcggaa
    35101 aagctgaata atttcttaaa accggatagt cctgcaaaga gattatccaa agagactata
    35161 tttttaatat agtttcgtgt aaaataatta tcgatgatat gggcatgtgg tgtaccttgt
    35221 gtacatcata tacctatcag acaaataaaa aatatattgt atttttagga ggacatttta
    35281 aaatggcaaa agaaaaattt gaccgttcta aaccacacgt taacattggt actatcggac
    35341 acgttgacca tggtaaaact acattaacag ctgcaatcac aactgtacta tctaagaaaa
    35401 acggcggcca agctatggct tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg
    35461 gaatcacaat ctcaacagct cacgttgagt atgaaacaga tacacgccac tacgctcacg
    35521 ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct gctcaaatgg
    35581 acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact cgtgaacaca
    35641 tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac aaagtagaca
    35701 tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac ctattaacag
    35761 aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg aaagctctag
    35821 aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt gacgaataca
    35881 tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa gacgtgttct
    35941 caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa gttcgcgttg
    36001 gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca gttactggtg
    36061 ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt ggtgctttac
    36121 tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa ccaggtacaa
    36181 tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac
    36241 gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca actgacgtaa
    36301 caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac aacgtaacaa
    36361 tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg
    36421 aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa ttcacttaag
    36481 ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt ctcaggatct
    36541 ttgttccgag cttttttatt tattgcagga tcaggaaaaa agacaggagt attataatag
    36601 aaggaacgcg aaataaaaga gtcagtattt tctaaatgaa actttatgaa aacaaaatgt
    36661 tttttacaag aaaaagcctt gctttgtgag tttatatata ttataatgac aaaggtgcgt
    36721 ttactttagt gaattgcaca gacgtaatca gaatgatttc gtcggctaag aaacgagagg
    36781 ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac ggaaattttc gtggagctat
    36841 gtctactttt caaaataggc gaaggaggga acaagatggc aaaacaaaaa attcgtatcc
    36901 gtttaaaagc gtatgaacac cgtattttag atcaatcagc ggataaaatc gtggaaactg
    36961 caaaaagaac tggagctagc gtgtctggtc caattccatt accaactgaa cgcagtcttt
    37021 acacaatcat ccgtgcgact cataaataca aagattcacg cgaacaattc gaaatgcgta
    37081 cacacaaacg tcttatcgac attgtgaacc caacaccaaa aactgttgat gctttgatga
    37141 agctagactt accatctggt gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata
    37201 atggaggtgt actcatgacc aaaggaatct tagggaaaaa agtgggaatg acacaaatct
    37261 tcactgaatc tggtgaatta attccagtaa ctgttgtaga agctactcca aacgtagtac
    37321 tacaagtgaa aacaatggaa acggatggct acgaagctat tcaagttggt taccaagata
    37381 tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca aacacggctc
    37441 ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag
    37501 aaattacagt agatgttttc caagcaggag acattattga tgtaacaggt actacgaaag
    37561 gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag ccgcggacca atggctcacg
    37621 gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac cgtgtattta
    37681 aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa aacttggaag
    37741 ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt cctggagcta
    37801 aaaaatcttt aatcactatc aaatcagctg tgaaagctaa ataattgtag gaagaaagga
    37861 ggaactaagg aatgccgaat gtagcattat tcaaacaaga tggaagccaa aacggtgaaa
    37921 tcacacttaa cgaagaaatc ttcggaattg aacctaatga aagtgttgtt tacgatgcaa
    37981 tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg
    38041 ttcgtggcgg tggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat
    38101 ctatccgctc accacaatgg cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt
    38161 acagctacaa acttcctaaa aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa
    38221 aagttgctga aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa
    38281 aagaattcaa acaagttctt gctaacttat ctatcgactc taaagtattg gttgttctag
    38341 aaaaaggtaa cgactttgct gctttatcag cacgtaactt accaaacgtt tctgtagtaa
    38401 cttctgataa tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa
    38461 ctgctcttac tcaaattgag gaggtgcttg cataatgaac ttactagacg taatcaaacg
    38521 cccagtgatc actgaaaaat caatgcttgc catggatgac aagaaataca cttttgaagt
    38581 ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt ttgacgttaa
    38641 agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc
    38701 aggatataca aaaaaacgtc gcaaagcgat cgttacattg actgaagatt caaaagaaat
    38761 ccaattattc gaagctgctg aataagcact aatagataac tcattaaatt aggagggaaa
    38821 aacgtggcga ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat gacaggttct
    38881 gattttgctg aaatcacaac atcaacacct gaaaaatcat tgttacaacc attgaaaaac
    38941 catgctggac gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg cggacacaaa
    39001 cgccaatatc gcgtgatcga cttcaaacgt aacaaagata acgttgttgc aactgttcaa
    39061 acgatcgaat atgatccaaa ccgttcagct aacatcgcgt tagtacacta tgaagatgga
    39121 gtgaaagctt acatcttagc acctaaagga ctagaagtgg gtatgaaact tgtttcaggt
    39181 ccagaagcag atatcaaagt cggaaatgct cttccattag aaaacattcc agttggtact
    39241 gttatccaca acattgaaat gaaacctggt aaaggtggac aattgatccg ttctgctggt
    39301 acaagtgctc aagtacttgg taaagaaggc aaatacgtat tgatccgctt gaactctggt
    39361 gaagttcgta tgatcttagc tgcatgccgt gctacaatcg gttctgtagg taacgaacaa
    39421 cacgaattaa tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa acgcccaact
    39481 gtacgtggta gcgtaatgaa ccctaatgat cacccacacg gtggtggtga aggtaaagct
    39541 cctatcggac gtaaagcacc agtttctcct tggggtcaac cagctcttgg ttacaaaact
    39601 cgtaacaaaa aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa taaataatta
    39661 ctttgaccat gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag tttaaagaaa
    39721 ggaccttttg tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag
    39781 aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt tgttggatat
    39841 accatcgcag tttatgatgg acgtaaacat gtaccagttt acatccaaga agacatggta
    39901 ggacacaaat taggtgaatt tgcacctaca agaacttatc gtggacatgc tgctgacgat
    39961 aagaaaacga aacgctaatt tgaggggagg ataaatccaa atggcagaac aaatcacatc
    40021 agctaaagca actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt tagtaattga
    40081 ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca caccaaacaa
    40141 atctgctgga atcattgaaa aagtattgat gtcagcagtt gcgaatgcag aaaataactt
    40201 tgacttagat gttgaaaact tggtagtaag cgaagctttt gttaacgaag gacctacaat
    40261 gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca atcaacaaac gtacaagtca
    40321 tatcacggta gtcgtatcag aaaaataagg agggataatc agtgggtcaa aaagtacatc
    40381 caattggaat gcgtgtaggc atcatccgcg actgggatgc taaatggtat gctgaaaaag
    40441 agtatgctga gttcttacac gaagatttaa gaatccgtaa atttatttca actaaacttg
    40501 ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa
    40561 ttcacacagc taaaccaggt atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa
    40621 gaaaagaatt aaacaaatta actggtaaaa gaatccatat caatatcgtg gaaatcaaaa
    40681 aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg
    40741 ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag
    40801 ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat
    40861 actcagaagg aactgttcct cttcatactt tacgtgcgga cattgattac gcatgggaag
    40921 aagcagatac aacatacgga aaactaggag ttaaagtgtg gatctatcgt ggagaaattc
    40981 ttccaactaa aaaaaacact gagaaaggag ggaaataatc atgttagtac ctaaacgtgt
    41041 aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt
    41101 agcattcggt gaatacggtt tgcaagctgt tgattcacat tggatcacaa accgccaaat
    41161 cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag tatggattaa
    41221 aattttccct cacaaatctt atactgccaa agcaattggg gtacgtatgg gttctggtaa
    41281 aggggcacct gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc
    41341 aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca aattaccaat
    41401 gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa
    41461 tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt
    41521 ttaatcttag attccaatta gcaacaggtc aattagaaaa cactgcacgt attaaagaag
    41581 tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga acaagctaag taattatgga
    41641 aggaggccat taagcgtatg actgaaggaa gaaatcaacg caaagtttat caaggtcgcg
    41701 tggtttcaga caaaatggat aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc
    41761 ctatctatgg caaacgcatg aactattcta agaaatacaa agtgcatgat gaaaacaaca
    41821 cagcaaaagt tggcgacatc gtgagaatta tggaaactcg tccattatca gctacaaaac
    41881 gtttccgttt gttggaagta gtcgaagaag cagttattat ctaataacac gagattttcc
    41941 tatatagatt gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc
    42001 gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg
    42061 gatctggtcg taaaactgct aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa
    42121 cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat
    42181 caggagctcg tcgtacagac ggttcatata ttaaatttga tgaaaatgct gcagtgatta
    42241 ttcgtgatga taaaagccct cgtggaacac gtatctttgg gccagttgca cgtgaactac
    42301 gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga agttttataa tcttgagaca
    42361 cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa gttaaagtta
    42421 tcactggaaa agacaaaaac aaagaaggcg ttgtactagc agcgttcccg aaaaaagata
    42481 aagttattgt cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct caagctgctc
    42541 ctcaaggtgg aattgttgag atggaagcgc caattcatgt ttctaacgtg atggtggttg
    42601 actctactgg tgtagctggc cgagttggct acaaagaagt agacggcaaa aaagtccgtg
    42661 tttctaaaaa aaccggtgaa gttttagata aataaaagtt taaggaagga gggtcttact
    42721 gaatgaaccg cctaaaagaa aaatatctta aagaagtaac tccatcattg atggaaaaat
    42781 ttaactatag ctctgttatg caaacaccta aagttgaaaa aatcgtcatc aacatgggtg
    42841 ttggtgatgc ggtatcaaac gctaaaaact tagataaagc agttgaagaa ttaactttga
    42901 tcactggtca aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga ttccgtttac
    42961 gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg agaaagaatg tacgaattct
    43021 tagacaaatt ggtttctgtt tcactaccac gtgtacgtga cttccatggt gtaagtaaaa
    43081 aagcctttga cggtcgtgga aactatactt taggtattaa agaacaattg atcttcccag
    43141 aagtagatta tgatttagta gataaagtac gtggtatgga catcgttatt gtaacaacag
    43201 caaactctga tgaagaatct cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat
    43261 aattaaagga ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg
    43321 caaaattttc aacacaagct tatactcgtt gcgaacgttg cggacgtcca cattcagttt
    43381 atcgtaagtt ccatctttgc cgtatttgct tccgcgaact tgcctataaa ggtcaaattc
    43441 ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca taagaaggag gtaaaagatc
    43501 aatggtcatg acagatccaa ttgcagattt tctaactcgt atccgtaacg caaacatggt
    43561 taaacatgag tctttagaag tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt
    43621 gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa gatgacaaac aaggcgtgat
    43681 ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat
    43741 ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg
    43801 tttaggtatc gcaatcattt cgacttctga aggtgttgtc actgataaag aagctcgtga
    43861 aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa tctacaagga
    43921 ggtgtcttta agtgagccgt atcggaaata aagtcgttgt tatccctgaa ggagtaacag
    43981 ttactcaaga cggaaacaac gttacagtta aaggaccaaa aggtgaattg actcgtactt
    44041 tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt aactttcact cgtccaaacg
    44101 acagtaaaga aatgaaaaca attcatggaa caactcgtgc gaacttcaac aacatggttg
    44161 taggtgtcag cgaaggtttc caaaaagcgt tagaactaat cggggttggg taccgtgcac
    44221 aattacaagg atcaaaactt gtattgaacg ttggttactc acatccagta gaaatcacac
    44281 caccagctgg agtaactgtt gaagtaccag ctaacacaca agtaattgtt aaaggtgcga
    44341 acaaagaaga agttggcgaa ttagctgcaa acattcgtgg aactcgtcct ccagaacctt
    44401 ataaaggtaa aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg
    44461 gtaaataatc gtttagaaaa attgcataga agccagcagc aggaaaaagg catttggctg
    44521 aagtttacct tcaaaaaaat acatttttac ttgtgccgca gcttctattt ttcgaaacgt
    44581 atttactgct tgaaatagta gtacaataaa aataaagagg tgacaattgt gattacaaaa
    44641 ccagataaga acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt
    44701 actgctgagc gcccacgctt gaacgttttt cgttctaaca aaaacatcta cgctcaaatt
    44761 attgatgacg tagcgggtgt aacgctagca agtgcctcta ccttggataa agaaatttca
    44821 ggtggaacaa aaacagaagc agcacaagct gtcggtaaac tagttgctga acgtgcagct
    44881 gaaaaaggca ttaaagtagt agtctttgac cgtggtggat acctttacca tggccgtgtg
    44941 caagctttag ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa ggaggaatac
    45001 cattcatggt ttatatcgat ccaaaacatt tggaattaga agaccgcgtt gttgcaatta
    45061 accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct ttagttgttg
    45121 tcggtgacaa aaacggacac gtagggtttg gtacaggtaa agctcaagaa gttcctgaag
    45181 caatccgtaa agcaattgaa gatgcgaaga aaaacttagt tgaagtacct atggttggtt
    45241 caacaatccc acacgaagta atcggtgctt ttagtggtgg tagaatccta atgaaacctg
    45301 ctgtagaagg ttctggggtt gctgctggtg gaccagttcg tgccgtatta gaattagctg
    45361 gggtagcaga tatcacttct aaatctttag gctcaaatac accaatcaac gttgttcgcg
    45421 caacagttga aggtttgaaa caattgaaac gtgcagaaga agttgcagaa cttcgcggca
    45481 aatcagttga agaattaatc ggataaggag gacaacaata atggctgaat taaaagttac
    45541 tttaaaacgc agtattatcg gacgtcctca aaaccaacgc gatacagtta aagcgttagg
    45601 tcttacaaag attaacagtt cagtggttaa gccttctaat gaagcaatta aaggcatgat
    45661 caatactgtt tctcacctag tggacgttga agaaatttaa tttagaaaaa tcataactag
    45721 aacattaagg aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct gaaggttcac
    45781 gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa actgccggac
    45841 gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga tttgaagggg
    45901 gacaaacacc attgttccgt cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag
    45961 actatgcagt cattaatctt gatgtattga accgctttga agacggagct gaagtaactc
    46021 ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa agctggaatc aaagtattag
    46081 ctaatgggga actaaacaag aaattaactg tgaaagcagc aaaattctct aaagcagctc
    46141 aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta gactattaaa
    46201 agatgctttt aaagtaaaag acattagatc gaaaatccta tttactgtat ttgtgttgtt
    46261 tgtatttcgc ctaggtgcac atattactgt accaggcgta aatgctgctt cattgcaaaa
    46321 cctttcttct ttgccatttt tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa
    46381 cttttccatt ttctcaatgg gggtttcacc atacattact gcttctatcg tcattcagct
    46441 tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg
    46501 taaaaaactg aatcaagcga ctcgatatct gacattagta ttggcattta tccaatctgt
    46561 cggtatcact gctggattca atcaatacgc tcaattaggt ttcgtgaata atcctaacgt
    46621 agcaacttac gtaacgatcg gtttgattct aacagccggt acgatgtttg ttacttggct
    46681 aggagaacag atcacagaaa aaggtatcgg aaatggcgta tcaatgatca tttttgcagg
    46741 tatcatttct agattgcctg aaagtatcag agaaatttat gaagattact ttataaatat
    46801 tgatccttca gacatatgga aatctgctat ttttgtggcc attttactag tagcggtctt
    46861 gttgatcgtg acattcgtaa catttttcca acaagctgaa cgcaagatcc cgatccaata
    46921 tacgaaacgc gtagctggcg cgcctacaag cagctatctg ccgttgaaag taaatgcagc
    46981 tggagttatt ccagttatct ttgctagttc gtttattgct acgccaaatg ctgttttaca
    47041 agcattgagc ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa
    47101 ctataataca gcacctggtg ctacgattta tacgattctg atcgttgcct tcactttctt
    47161 ctacgccttt gttcaagtaa accctgagaa attagcggaa aacttgcaaa agcaaggaag
    47221 ttatatacca agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa gattactgat
    47281 gcgtttaagt acagttggtg cagtcttcct tggtttagtg gcattgcttc caatcattgc
    47341 tcaaatggtt tgggatcttc cacaatcaat cggattaggc ggtacaagct tactgatcgt
    47401 tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata
    47461 tgttggattt attgagtaag aaagcgtgtt gaggtttccc ctcaacacac gttctcacac
    47521 atttattagg agggaaaaca atgaacctca ttttaatggg gctgccaggc gcaggtaaag
    47581 gaacacaagc tgaaaaaatc attgatgctt atggtattcc gcacatctca acaggagata
    47641 tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag tcttatatgg
    47701 ataaaggtgc attagttcct gatgaagtaa caaatggaat cgtgaaagag cgactagctg
    47761 aaccagatac agagaaaggc ttcttattag atggctttcc acgtacgcta gatcaagcaa
    47821 aagctctgga cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc atcgatatcc
    47881 atgttggtga agaaatcttg gtcgaacgtc tagccggccg atttatctgc agcaattgtg
    47941 gagcaactta ccataaaatt ttcaacccta caaaagttga agacacatgt gatcgttgcg
    48001 gtgggcatga attctatcaa agagaagatg ataaacctga gacggttaaa aatcgtctgg
    48061 ctatcaacat caaaaatagt gaaccaatct tggcttatta taaagaacaa ggtttgctaa
    48121 acactattga tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg
    48181 aaaaatagca cgattgcccg tagccgactt gcattatgca gttactcttg tcaagttcaa
    48241 taaattatga tagaatatgt cagtccgact tctagagaat cctctagatg atccattttg
    48301 agaattttga ggtgggaact gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag
    48361 tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc
    48421 gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa acggacacca agttcttgct
    48481 actgtttcag gtaaaattcg tatgcattac atccgtatct tacctggtga caaagtaacg
    48541 gttgaattgt ctccatacga tcttaaccgt ggccgtatta cttatcgctt taaataattg
    48601 tactccgtaa aactacagga ggtattatca tgaaagtaag accatcagta aaaccaatgt
    48661 gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc
    48721 caaaacataa acaacgtcaa ggataattgg aggtgctata aatatggctc gtattgcagg
    48781 agtagatatt cctcgtgata aacgtgtagt agtttcactt acttatatct atggtattgg
    48841 taacactact gctaaaaaga tcttagctga tgctggtgta tctgaagatg ttcgcgttcg
    48901 tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa gtagataaat taaaagttga
    48961 aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa tcggttctta
    49021 ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga aaaacaatgc
    49081 acgtactcgt aaaggcccag ctcgcgctat cgctggcaag aaaaaataat tcttaagtga
    49141 aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa
    49201 tatagaagca ggtattgcac atatccattc tacattcaat aacactatca tcatgatcac
    49261 tgatactcac ggaaatgcat tagcgtggtc atcagctggt tcattaggtt tcaaaggaag
    49321 caaaaaatca actccttttg ccgctcaaat ggcagcagaa actgctacaa aagcagcaat
    49381 ggaacacgga ttaaagactg ttgaagtaac agttaaagga cctggttctg gacgtgaagc
    49441 agcaattcgt tcattacaag ctacaggttt agaagtgact gcaattcgtg acgtgactcc
    49501 agttcctcat aatggtgccc gccctccaaa acgccgtcgt gtttaatgag tgccgctcat
    49561 tttgagtttt atcagtaacg tcattgaaca agacactttt cgttttgaaa ggggtaaaga
    49621 taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg
    49681 gcaagttcat cgttgaacct cttgaaagag ggtatgggac tactttaggg aattccctac
    49741 gtcgtatttt attatcttct ttgccaggtg ctgccatcac taatattcaa atcgatggtg
    49801 tactgcatga attctctacc atcaaaggtg ttagagaaga cgtcactcaa atcatcttga
    49861 acattaaagg cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata
    49921 ttaccggacc tgctacagta actgctggcg atattatcgt tgatagtgat gtagaaattc
    49981 ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa
    50041 cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa
    50101 ttggcgttct tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg
    50161 agaacacacg tgttggacac cgtgaagatt ttgataaatt gacgatggag atttggacag
    50221 atggttcaat tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg actgaacatt
    50281 tggatatttt tgtgaactta actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag
    50341 aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga agagttagac ttgtctgttc
    50401 gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt acaaataaat
    50461 ctgagcctga aatgatcaaa gtccgtaact taggacgtaa atcattagaa gaagttaaag
    50521 caaaattgca cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga
    50581 gggaaaccaa cgtgagttat cgtaaactag gacgcacatc tagccaacgt aaagcgatgt
    50641 tgcgtgattt aacaactgat ttattaatca acgaacgtat tgtgactact gaagctcgtg
    50701 cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc
    50761 atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa
    50821 atgaggacat cgtcattgaa tcagctttac aaaaactgtt caacgatatt gcacctcgtt
    50881 acgcagaacg tcaaggcgga tacacacgca ttttgaagac agaaccaaga cgcggtgatg
    50941 gtgcaccaat ggttgtctta gaacttgtct aaaatcacac taataacatc actttataga
    51001 tgattctttt agagtgttat gatgttggaa gatatttttc cgagtctagc tcagcgctgc
    51061 tcctcaagcg aatatcgctt ggggagtaag cactcatcat aaagtgtttt tttatacctt
    51121 caattacctg tcatgacaga tatgtttgaa ggcataaaaa aacattttaa gcgagaaata
    51181 attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac tttaggatga agaaggagta
    51241 atcatggagc caattattga attagaaaag ataaattata aataccagcc agatgatctt
    51301 cgccccgcat tgaaagatgt ctcctttaca attgataaag gtgagtggat cgctattatt
    51361 ggacacaatg gttcaggaaa atcgacactt gccaaaacga tcaatggatt gcttctgcca
    51421 gaatcaggga tcgtgaaagt gggaaatcaa atattggatg aagaaaatat ttggacgatc
    51481 cgacagatgg ttggaatggt ttttcaaaat ccagacaatc agtttgttgg ttcgacagta
    51541 gaagatgacg ttgcttttgg tcttgaaaat caagggattc ccagagaaga aatgctagtg
    51601 cgtgtgaaag atgcacttga gaaagttaga atggctgagt ttgcttcacg cgagcctgct
    51661 cgtttatcag gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc cttacgacct
    51721 gatatcatta ttttagatga agcaaccagt atgctagatc cagaaggtcg tgaagaagtg
    51781 atctctacga tcaagaagat caaagaagaa agtcaattga ccgttatatc tatcactcat
    51841 gatattgatg aagcagctaa tgccaatcgg atactagtga tgagacaagg cgaacttgtc
    51901 cgtgaaggta caccaaaaga aattttttct gctggtcctg aactgatcga cttaggtcta
    51961 gatctaccat ttcctgaaaa actgaagagc gcactgaaag aacgtggggt tgatgtgccc
    52021 agtgaatata tgacagaaga aaggatggtg gattggttat ggacatccgt tttgaacaag
    52081 tagacttcac ttatcagccg aatacaccat ttgaacaaag agccttattt gacatcaata
    52141 tgacgatcaa agaaaacagt tatactgcat tagtagggca taccggaagc gggaaatcaa
    52201 ccttacttca gcatttgaat gcgcttgtta agccaacaag cggtacagtc catattggag
    52261 aacgagatat tcagccggat acggataata agaatttaaa gcccatccga aaaaaagttg
    52321 gcatcgtctt tcagtttcca gaagcacagc tattcgaaga aacggtagca aaagacattg
    52381 ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa
    52441 cattagaaca agttgggctg gatgaaagct atttggaacg ttcgccattt gaactttcag
    52501 gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg
    52561 tattggatga accaacggca ggacttgatc cccagggcag aaaagaaatg atggagatgt
    52621 tttggcgtct tcataaggaa cagcagatca ctattgtatt agttacgcat ttaatggatg
    52681 atgtagccaa ttttgcagat tatgtctatg tgttggaaaa gggacgaatc gttaatagcg
    52741 gggagccgca agaggtcttt caaaacattg aatggctaaa agaaaaacaa ctaggcgtac
    52801 ctactgccac cgagtttgca gaagaattaa tggctaaagg gatgaacttt gctaccttgc
    52861 ctttgacagc tgaagaatta gcagacgcta tcgtccatta tgctggagga aatgcccatg
    52921 atgaataaat tgatatttgg tcgttatatg cctggagatt ccttgatcca tcgattggat
    52981 ccccgagcta aattattagc cagtttttat tttatcggaa tcattttttt agcaaataat
    53041 tgggaaacgt accttttgtt ggcatttttc acattacttt ctgtcttctt gtcaaaagta
    53101 gatttgaaat tttttatccg aggcattcgc ccattgatct ggctaatcct cttcactgta
    53161 gctttgcagg tcttgtttac aagtggcgga gaaacttatt ggagttgggg tgttttccat
    53221 atcactgaat ttggtgtgat gaacggcctc tttattttct gtcgattcgt gttgatcatt
    53281 tttatgtcga ctctactcac gcttactaca ccgcctttgg aattatcgga tgcgattgag
    53341 tacttgcttc gtccattgaa agccgtacgg ttcccagtcc acgaaatttc attgatgctg
    53401 tcgattgctc ttcgttttgt gcctacattg atggacgaaa cagaaaagat catgaacgct
    53461 cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc
    53521 attcctttgc tgattccttt atttgtcagc agcttcaacc gtgcggaaga tcttgcgact
    53581 gctatggaag caagaggcta caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat
    53641 tggcataata gagacacgat ggttatcatt gcttttgtat tattaactgt gctactgatc
    53701 tttttaagag gataaacgtt cagtgggccg ctatctattc ttttcttgct gatctgtttg
    53761 caagaaagga tgcaagcagt gtatgacaag aaatattagg gtctgtggca gactaaggtc
    53821 acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa attttataga aaaatcatta
    53881 ttccagataa ccttttagga acaaaggaga gaaaaacatg gtacgttaca aagcaatcat
    53941 tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag ccaaatgggc gtactgttca
    54001 agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg
    54061 ttcgggaagg acagatgccg gagtacatgc gatcgggcaa gtaatccatt ttgattatcc
    54121 agaggagcgt ccgttagaac gaatgcgttt cgcgctagat acccagtcac cagaggatat
    54181 tgctgtaaga caagtagaaa ttgtttcaga agacttccac gcaagatatt tggtcaaaga
    54241 aaagacgtat caatttcgag tggatatagg gaaaccacgc agtccttttc gacgccatta
    54301 tgcaagttat tttccttacc cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt
    54361 attagggact cacgatttca cctctttttg tgcatcagga agctcgatag aggataaaat
    54421 acgtacgatc tacgaggcta agatggaggt aaatgaaaca aaagacgaat tgttgtttac
    54481 atttagggga aatggcttct tgtataagat gatacggatc ttagtaggaa cgcttttgaa
    54541 aattggcaat ggccgattgc ctgaagatag tatcccagat attattgcga aaaaagatcg
    54601 taatgcagca ggacctacag cacaccctga aggactttat ttgtatgaag tgaaatatga
    54661 ataagaggtt atgatagaag tggtcagctt caagaaataa gaaggtattt acgaaaatta
    54721 cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga agctacttct ggaacaccgt
    54781 ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaaagagca atttcaaaaa
    54841 acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat aagtgaatca
    54901 tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc
    54961 ttggtgcgga cagtctgaac aggttccatc gtttctttat tttctacgtc ttcttctgtg
    55021 tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg ccataaaagg ggcgattgcg
    55081 gtcaaggtac cagacataaa gaataaacaa tgcaatacat aggacaaaaa ggattgcacc
    55141 tatcgtaaat ccttcaggca agtaactgaa agtgacagta tgtttacctg ctggaacttt
    55201 tatcatcaag aatgcgtctt tgaaattgct gaccttgact ggtttcccat caattttagc
    55261 tttccatcct ttgtcataag gaatagttgt caccaacatg gaatcttcag cggtatttac
    55321 agtcccagaa gcttttctgc cagtggcttc catttcgaca cctttttctt tgatctgatt
    55381 cattgcggct tgataggcta caacatctag accaacgact ttaggctcca tgaaacttat
    55441 ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt gtctcttgag gataatagcc
    55501 aagatcatag tattgaccag aaatgttgat ttgcgttttt cgcgatgtac cattgacagt
    55561 gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt gggaacaaac ttaagtaagc
    55621 ttgtgtattt gcaggcacat ggacttccca agtgagctct tttggctggt tatctgctgt
    55681 ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc gtattcaact gattcttcaa
    55741 agtaggtgta tagaatgtaa aatactgttc atttagacca gacaatgtat tgatcaagtt
    55801 tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca tcattaacgg acaacggtgc
    55861 agtaaatgct agtggcaatg cattgacatt ctgatacagt gtatagtcgc cgctttgacc
    55921 agcttggcgg aagccatatt tactgaacgt gctttcgttt ttagaaatgt tatatttgat
    55981 tcctgtaaac ccatccatca gcaaagtatt attgttataa cggatattca aatttgtccc
    56041 tcttgaacgg aaacctaatt tatccaggta agaggaagaa ttacgatttc ttatagaaga
    56101 aaacaaactg ataccactgt aaccatagtt gatcgaatcg tttgcagata ctggatccag
    56161 attttccaag cggtaaaacg agtcggaatc tttttttgtt tcatttacaa gagctttcaa
    56221 gtcaggataa ggatcagtat ataaactgcg ggaagcataa ttccaatcat caagaatgcc
    56281 atggatcata ctgttcgtat tgaccagtgc ttcagcagac atgatcaata ataacaatat
    56341 actcagatga ttgaatgaaa gttttttatg ctgataaaat cccacagcaa aagcataaat
    56401 ccctaagaaa accactgtca atacaaagga agtcatcgtc acataggtgt agcttccttc
    56461 aggtttgatg ccccatgcca ccgcaaagag agcagctacg accaaaatga ttcctaacaa
    56521 cagcccacga tcgtcttgtt ctatcttttc ccatcctttt cctgcaagca ggagcactaa
    56581 gaaagagaac agataggcat aacggaataa aaacatattc ggtgcatgca taccatgcca
    56641 gaacaaattc aatggaacaa gataaaaact tgcgataagt atggcgaaaa gactgccgta
    56701 caaaaattta tttttcaacg gtactttccg actggcaaaa taaaacagac agaggactaa
    56761 tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat tttgtcgtgt cataaacacc
    56821 aatcatattt ttcatgatta gatccagata agaagtcgct tctgttttca acgtagtgat
    56881 ctgactcagt gcctcgccat ttgaccgaag atccagcact gctggcaata caatgatcat
    56941 tgaagcgccg cctgccagta aagaagtgat accataagga acaatcgttt ttttatattc
    57001 tgaccagttt gtcaatagac gggcgataaa atataagaaa gaaaatacgc caatcataaa
    57061 acccatataa aaactggata aaaacaagag taaataactg ataaataaaa gtttcggccg
    57121 tttaagatcc ataacacgat gaatccccaa aatcactaat ggaagataaa caaatgcatc
    57181 cagccacatg atgatctccg aatgtgcagt gataaatgag atcaatgcgt aactgacgct
    57241 aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt gcaagaaacc aaaaagccaa
    57301 tccggcacag ccgaccttaa gcaaagtcag aacatacagt gcatctggca tcatctggtt
    57361 tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga tagtaagaaa ttaaagataa
    57421 ataattcaat cctagagaag cattccatgt ataaaaaata ctttgctttc caagaagaac
    57481 atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag gcatcacttg ccaaaatact
    57541 tctgctgctt ccaggataga ttccgatact tagataaaca agtgctaaaa tcaaaaacgg
    57601 aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta aagaatgact ttaaattttt
    57661 tttcatattt attttccacc aaatttctat gaagttgtat tcagtttatc atatccttgt
    57721 ttttctctgc ttaaaaagag tctagttttc acaaaaaaat aaaatttaga ataaataact
    57781 tcataatttg ttcgtataac aaaaataaac gcaaaaaaag ggtaggaatc aaaagtccca
    57841 cccttaaaaa tttttattaa ataaaaaacc gttcactatc tgaacggtcc aagtatcatt
    57901 caattaaacg cgttcaactt ttcctgattt caaagcacga gttgacaccc aaactttttt
    57961 aggttttccg tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt
    58021 tgagttcata gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg taaagtaaca
    58081 aacttttgcc atggtaattt cctcctttgc cttgatttgg agcaatagaa taattcagct
    58141 cataccagac taacatatca tatagaatgg gaatttgcaa gtacattttc atagatacaa
    58201 ttgacaattg agaagaataa ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt
    58261 ttttgagtac tttgggtaac tgccttttat ttcaagttgc gctatgataa aatatgatag
    58321 aagaaaaatg ggagtttttt acccgttaag gaggctcatt catggctgta aaaattcaaa
    58381 cgtcagctgg aaccattgaa attaccaatg atgtaattgc aacagttgtt ggtggcgccg
    58441 cgacagatgt gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta
    58501 ctgagatttt gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa gaagataatg
    58561 gtattgctgt agatgtttat acgattatca gctatggtac gaaaatctct gaagtttccc
    58621 gcaacgtcca agaaaaagtg aaatacaatc tagaggcatt attaggcgta actgccaatt
    58681 cagttaatgt ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga gactggctgg
    58741 taactcagca gtcgatctga aggaggatta tctaggtgag tgtaacagaa ataagtgcaa
    58801 gccagttcca ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg
    58861 tcaattcatt aaatgtattc cctgtgccag atggcgatac gggaacaaat atgaatctat
    58921 ctatgactag tggagctaag gcagtagtag attcaacctc agaaaaagta ggagaattag
    58981 cagcaatgct ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt
    59041 ctcaattgtt tcgtggattt tctaagcaaa ttccagatgt tgtctcatta aatgcttctg
    59101 atcttgctgc agccttcact catggagttg aaactgcgta taaagcagta atgaaaccag
    59161 tagaaggaac gattttgaca gtagctagag aagcagcgaa agctggagaa aagaaagcaa
    59221 aaagtactga tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca aagcgtgctt
    59281 tagctaaaac tccagacttg cttccagtcc taaaagaagt cggtgtcgta gacagtggag
    59341 ggcaaggact gctgtttgtt tatgagggct ttttaagtgc attgaatgga gaattccaag
    59401 cggatgaaac atatgaacct tctccagcag aaatggatga tatggtcaat gcagaacatc
    59461 atcgcagtgt ccaaggtcaa atggcgacag aagatatcca ttttggttac tgtacagaaa
    59521 tcatggtaaa aatcggagaa ggacctacag tcgatagtac attcgattac gaagaattcc
    59581 gtaactattt agatggtata ggagattctt tacttgtggt caatgatgac gagatcatca
    59641 aagtccacgt gcatacagaa aacccaggag aagtcttgaa ttatggacaa aaattcggtt
    59701 cattagtcaa aatcaaagtc gacaacatgc gcttgcagca tgagacgatt ttggaacacg
    59761 atgaagaagt gaacgagttt gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag
    59821 cgatcgctgc tggtaaaggc gtccaagagc tgtttgaaag tttaggtgct aattatgtta
    59881 tcagtggtgg acaaacaatg aatccaagta cggaagacat tgttaaagcg attcaagaag
    59941 taaacgcaga taaagtgatt gtcttgccaa acaataagaa catcttcatg gcagctgatc
    60001 aagcagcaga agtagcagat atcccagtag cagttgttcc ttcaagaacg atttcccaag
    60061 gattgacagc tatgctttca ttcaatgatc aagcttcttt agaagaaaac aaagaaacga
    60121 tgacagaaat gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc agagatacct
    60181 caattgacgg tgtagagatc aaagaaggag actttttggg catgatcgat ggaaaaatcc
    60241 tgatctctaa cccagatatc acagcaacag cgttaagtac actagaacaa atgatcaatg
    60301 aggacacaga gatcgtaacg atcttggcag gtgatgaagg aacagttgaa caagcaaatg
    60361 aatttgctga gaaactaaca gaaaaacatc ctgatttgga aatcgagatc caccaaggag
    60421 atcagcctgt ctatccttat ctattttctg ctgaataatt tctgtgtcag aatatctttc
    60481 taacacagta ttcattcata aaaggccttc ggttagtgat caggtatgat cgctgaccaa
    60541 gtcctttttt tgttaaagga gggaggaaaa atttatgcgt tctttagatg atccaatcac
    60601 tatgctggca ggagtcggag aaaaaagagc agctagtctt gccagcttgg gcatccaaac
    60661 gattgaagac ttactgactt attatccttt tcgctacgaa gatattcagg aaagaaatct
    60721 aaacgagatc caggatcaag aaaaggttac tttgaaagga atcgtagtct cgcctcctgt
    60781 gatgaatcga tttggttata aaaaaagccg attgcaattt cgtatgatgc aagaacatga
    60841 tgtgttcaac gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga
    60901 ggaaatcgcc gtatacggta aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat
    60961 tttaggttct caaacaatgg atgacttttc gccgatatat cacgtcaaca aatcaattcg
    61021 tcaaacgaca ttagttgatc taatcaaaaa agggtttcaa gaatacggca cattgattga
    61081 agaaaatctg ccaaacaacc ttgtggagaa atatcgactg cttgaccgag cttctgctgt
    61141 aagaagtatg cattttccaa aagaccacga agaaaaccat caagccaaac gacgtgtcgt
    61201 ttttgaagag ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc
    61261 agaaacgaat ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa
    61321 gctacctttt gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct
    61381 tcgcagtccg cagcatatgc agcgactttt gcagggagat gtaggtagcg gaaaaacagt
    61441 ggttgccgcg attgcgttat acgctaccgt tacagcaggt ttccaaggtg cgttgatggt
    61501 gcctacagaa atcttggcac aacaacatat ggaaagcttg caacagctgt ttgatcccaa
    61561 tgaagtaacc atcgcattga ttacggggtc aacgaaagca aaagaacgac gtgagctcct
    61621 tgaaaaacta gagcaagggg aaattaacgt cgtgatcggc acacatgcat tgatccaaga
    61681 tggagtcgaa tttcagaatt taggtctagt tattacggat gagcagcacc gttttggcgt
    61741 gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc
    61801 gactccgatt cctagaacat tagctattac agcatatggt gagatggatg tatctatcat
    61861 tgatgaactt ccagctggac gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt
    61921 ggacagtgta ctggattgga cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat
    61981 ctgtccgctg attgaagaat cagaagcatt agatgtaaaa aatgcagtgg aaatctatga
    62041 aaaacttcgt gaactatttg caccaaaata tgaagttggc ttgttacatg gtaaaatgaa
    62101 gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac aaccagatgc agatattagt
    62161 ttcgactact gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat
    62221 ggatgcggat cgatttggat tggcacagct gcaccaactt cgtggacgtg ttggacgcgg
    62281 aagtgatgct tcctactgta tcttagttgc taatcctaaa aatgaactag gtgtggaacg
    62341 gatgaaaatc atgacagaga ccaccaatgg ttttgtacta agcgaaaaag atctggaatt
    62401 acgtggtcct ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc
    62461 tgacttggtg acggatggaa atgttttgga ggtagccaga gaagaggctg cagccctttg
    62521 gcaaataaag gattggcaga tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat
    62581 agaaaatcag gataatcgtt atttcgatta gcagactgtg ctatacttta tactgatgac
    62641 ggaaaaagga gtggaaacaa tgaaaattgc cgtagatgca atgggcggag atcacgcacc
    62701 tcaagcaatt gtagaaggcg tggctttagc gcagcaagat tttccagaag tagaattctt
    62761 actttacgga aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa acatcacgat
    62821 tatccataca gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa
    62881 aaagactgca tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt
    62941 attttcagca gggaatacag gcgcgctact agcagcaggg ttgttcatcg taggacgtat
    63001 caaaaatatc gaacgccctg gcttgatgtc taccttgcca gtagtaggga aagaaggtgc
    63061 cggtttcgat atgctggatc ttggcgctaa cgcagaaaac aaaccagaac atttgttgca
    63121 atacggtatt ttaggttctt tttatgcacg taaagtccga ggtatcggac gtcctcgtgt
    63181 cgcactctta aacaacggaa cagaagcaac aaaaggtagt gaagtgacga aaaaagccta
    63241 tgaactcctt cagaatgaaa caagcttgaa ttttattgga aacgtagaag ctcgtgagct
    63301 actcaatggt gtggctgatg tagtggtcac agatggattt acaggcaacg ctgttttgaa
    63361 atctattgaa ggaacagcaa tgaatatgat gaatctatta aaatctgcta ttctaaatga
    63421 agggatcaaa ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc
    63481 agaaatggat tattccaaac atggcggtgc cgttttgttt ggcttgaaag caccagtcat
    63541 taaaactcat ggagcaacag gccccgatgc tgttcgctat acgatccgcc aaatccacac
    63601 aatgcttgag acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata
    63661 gtatttggat atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat
    63721 gccagtaaaa ttagccatgg tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa
    63781 agttgactcg cgaagaagta tttaacaaag tagcgaaaat tatttcaaat cattttgaga
    63841 tagataccga taaagtgacg gatgaattga acatcaaaga tgatttaaaa gctgattcaa
    63901 ttagtattat ggaattcgtt ttagagcttg aagatgaatt cggtacggaa atttcagatg
    63961 aagacgctga acaaattgaa acagtcggtg gcgcagtaga ttatatcagc agccatctta
    64021 aataatttca aataaaagca aagaaggata aaaagctgca tgcagcttt
//
LOCUS       DACDCJ010000005        61041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDCJ010000005 DACDCJ010000000
VERSION     DACDCJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.005"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(32..1423)
                     /gene="sufB"
                     /locus_tag="IV038_000331"
     CDS             complement(32..1423)
                     /gene="sufB"
                     /locus_tag="IV038_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP8340582.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1428..1898)
                     /locus_tag="IV038_000332"
     CDS             complement(1428..1898)
                     /locus_tag="IV038_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP8340583.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1885..3120)
                     /locus_tag="IV038_000333"
     CDS             complement(1885..3120)
                     /locus_tag="IV038_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8340584.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="IV038_000334"
     CDS             complement(3117..4403)
                     /gene="sufD"
                     /locus_tag="IV038_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP8340585.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="IV038_000335"
     CDS             complement(4416..5189)
                     /gene="sufC"
                     /locus_tag="IV038_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP8340586.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5386..6234)
                     /locus_tag="IV038_000336"
     CDS             complement(5386..6234)
                     /locus_tag="IV038_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8340587.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6258..6944)
                     /locus_tag="IV038_000337"
     CDS             complement(6258..6944)
                     /locus_tag="IV038_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340588.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6937..7971)
                     /locus_tag="IV038_000338"
     CDS             complement(6937..7971)
                     /locus_tag="IV038_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8340589.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(8366..8704)
                     /locus_tag="IV038_000339"
     CDS             complement(8366..8704)
                     /locus_tag="IV038_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP8340590.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8706..9062)
                     /locus_tag="IV038_000340"
     CDS             complement(8706..9062)
                     /locus_tag="IV038_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP8340591.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9068..10255)
                     /locus_tag="IV038_000341"
     CDS             complement(9068..10255)
                     /locus_tag="IV038_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8340592.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10446..11291)
                     /locus_tag="IV038_000342"
     CDS             complement(10446..11291)
                     /locus_tag="IV038_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8340593.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11525..12490
                     /locus_tag="IV038_000343"
     CDS             11525..12490
                     /locus_tag="IV038_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP8340594.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12867..13910
                     /locus_tag="IV038_000344"
     CDS             12867..13910
                     /locus_tag="IV038_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP8340595.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13972..14436)
                     /locus_tag="IV038_000345"
     CDS             complement(13972..14436)
                     /locus_tag="IV038_000345"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP8340596.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14682..15629
                     /locus_tag="IV038_000346"
     CDS             14682..15629
                     /locus_tag="IV038_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8340597.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15840..17912)
                     /locus_tag="IV038_000347"
     CDS             complement(15840..17912)
                     /locus_tag="IV038_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8340598.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17845..18222)
                     /locus_tag="IV038_000348"
     CDS             complement(17845..18222)
                     /locus_tag="IV038_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP8340599.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18351..18911)
                     /locus_tag="IV038_000349"
     CDS             complement(18351..18911)
                     /locus_tag="IV038_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8340600.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19061..20353
                     /locus_tag="IV038_000350"
     CDS             19061..20353
                     /locus_tag="IV038_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8340601.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20413..21531)
                     /locus_tag="IV038_000351"
     CDS             complement(20413..21531)
                     /locus_tag="IV038_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP8340602.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="IV038_000352"
     CDS             complement(21545..22966)
                     /gene="arcD"
                     /locus_tag="IV038_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP8340603.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="IV038_000353"
     CDS             complement(23212..24333)
                     /gene="mnmA"
                     /locus_tag="IV038_000353"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP8340604.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24737..25336
                     /locus_tag="IV038_000354"
     CDS             24737..25336
                     /locus_tag="IV038_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP8340605.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="IV038_000355"
     CDS             complement(25474..26760)
                     /gene="efmA"
                     /locus_tag="IV038_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP8340606.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27031..27375)
                     /locus_tag="IV038_000356"
     CDS             complement(27031..27375)
                     /locus_tag="IV038_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP8340607.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27512..28648)
                     /locus_tag="IV038_000357"
     CDS             complement(27512..28648)
                     /locus_tag="IV038_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8340608.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28837..29811)
                     /locus_tag="IV038_000358"
     CDS             complement(28837..29811)
                     /locus_tag="IV038_000358"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP8340609.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29891..30766)
                     /locus_tag="IV038_000359"
     CDS             complement(29891..30766)
                     /locus_tag="IV038_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP8340610.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGCM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30896..32803
                     /locus_tag="IV038_000360"
     CDS             30896..32803
                     /locus_tag="IV038_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP8340611.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33344..36622)
                     /locus_tag="IV038_000361"
     CDS             complement(33344..36622)
                     /locus_tag="IV038_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP8340612.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36893..37438)
                     /locus_tag="IV038_000362"
     CDS             complement(36893..37438)
                     /locus_tag="IV038_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8340613.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37450..38142)
                     /locus_tag="IV038_000363"
     CDS             complement(37450..38142)
                     /locus_tag="IV038_000363"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP8340614.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38161..38445)
                     /locus_tag="IV038_000364"
     CDS             complement(38161..38445)
                     /locus_tag="IV038_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340615.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38458..39021)
                     /locus_tag="IV038_000365"
     CDS             complement(38458..39021)
                     /locus_tag="IV038_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8340616.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39249..39911
                     /locus_tag="IV038_000366"
     CDS             39249..39911
                     /locus_tag="IV038_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP8340617.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40035..41231
                     /locus_tag="IV038_000367"
     CDS             40035..41231
                     /locus_tag="IV038_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP8340618.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41332..42708)
                     /locus_tag="IV038_000368"
     CDS             complement(41332..42708)
                     /locus_tag="IV038_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8340619.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42745..43611)
                     /locus_tag="IV038_000369"
     CDS             complement(42745..43611)
                     /locus_tag="IV038_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP8340620.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43700..44725)
                     /locus_tag="IV038_000370"
     CDS             complement(43700..44725)
                     /locus_tag="IV038_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8340621.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44743..45417)
                     /locus_tag="IV038_000371"
     CDS             complement(44743..45417)
                     /locus_tag="IV038_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8340622.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45601..46494)
                     /locus_tag="IV038_000372"
     CDS             complement(45601..46494)
                     /locus_tag="IV038_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8340623.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46568..48265)
                     /locus_tag="IV038_000373"
     CDS             complement(46568..48265)
                     /locus_tag="IV038_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8340624.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48258..49571)
                     /locus_tag="IV038_000374"
     CDS             complement(48258..49571)
                     /locus_tag="IV038_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8340625.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49571..53089)
                     /locus_tag="IV038_000375"
     CDS             complement(49571..53089)
                     /locus_tag="IV038_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8340626.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53382..53840)
                     /locus_tag="IV038_000376"
     CDS             complement(53382..53840)
                     /locus_tag="IV038_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8340627.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54137..54655)
                     /locus_tag="IV038_000377"
     CDS             complement(54137..54655)
                     /locus_tag="IV038_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8340628.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54657..55274)
                     /locus_tag="IV038_000378"
     CDS             complement(54657..55274)
                     /locus_tag="IV038_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP8340629.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(55271..56125)
                     /locus_tag="IV038_000379"
     CDS             complement(55271..56125)
                     /locus_tag="IV038_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8340630.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(56122..57033)
                     /locus_tag="IV038_000380"
     CDS             complement(56122..57033)
                     /locus_tag="IV038_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8340631.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57074..58408)
                     /locus_tag="IV038_000381"
     CDS             complement(57074..58408)
                     /locus_tag="IV038_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8340632.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58616..59446
                     /locus_tag="IV038_000382"
     CDS             58616..59446
                     /locus_tag="IV038_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP8340633.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59690..59939)
                     /locus_tag="IV038_000383"
                     /pseudo
     CDS             complement(59690..59939)
                     /locus_tag="IV038_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(60130..60324)
                     /locus_tag="IV038_000384"
     CDS             complement(60130..60324)
                     /locus_tag="IV038_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340634.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(60829..61014)
                     /locus_tag="IV038_000385"
     CDS             complement(60829..61014)
                     /locus_tag="IV038_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340635.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT        17274 a        13130 c        10964 g        19673 t
ORIGIN      
        1 tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc atctcataac
       61 tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa
      121 caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat
      181 agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc
      241 tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg
      301 tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc
      361 gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat
      421 ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg
      481 cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca
      541 tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt
      601 ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac
      661 ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg
      721 aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga
      781 tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg
      841 gaacatcgac tttgacggct tttggcacgt aaataaaggt cccacctgac catacggctg
      901 aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga
      961 ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt
     1021 cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg
     1081 ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt
     1141 ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg
     1201 catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg
     1261 tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca
     1321 tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat
     1381 cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc
     1441 ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc
     1501 caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca
     1561 gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct
     1621 acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag
     1681 gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg
     1741 tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc
     1801 ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc
     1861 acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc
     1921 catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc
     1981 gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc
     2041 tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc
     2101 caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa
     2161 tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg
     2221 gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc
     2281 ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta
     2341 taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca
     2401 ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa
     2461 agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc
     2521 accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg
     2581 attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt
     2641 aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt
     2701 caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc
     2761 catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc
     2821 tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc
     2881 atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc
     2941 taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc
     3001 ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc
     3061 atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat
     3121 ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt
     3181 gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc
     3241 aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca
     3301 tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct
     3361 ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc
     3421 aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc
     3481 aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt
     3541 tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa
     3601 tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca
     3661 gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat
     3721 tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata
     3781 ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat
     3841 tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca
     3901 tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg
     3961 aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt
     4021 actgcttttt tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac
     4081 aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc
     4141 catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg
     4201 cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc
     4261 acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag
     4321 gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa
     4381 tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg
     4441 attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca
     4501 gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata
     4561 tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt
     4621 tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg
     4681 tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt
     4741 tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt
     4801 aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca
     4861 tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca
     4921 cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct
     4981 aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc
     5041 atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt
     5101 tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca
     5161 tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc
     5221 actcatttta gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc
     5281 ttttatttat tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga
     5341 aataagccat tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc
     5401 ctttactggt ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg
     5461 gtgaagcact tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc
     5521 aataatattc acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt
     5581 caaccctgcg ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg
     5641 gtacgtagaa gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt
     5701 ttcttcaata tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa
     5761 gccagcatct tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat
     5821 gattgttgca ccatctttta attcggaaac atctttgact tttttagagt acaagcccat
     5881 tggttcgatg tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt
     5941 gttcaaataa ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg
     6001 gttcggtaat acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa
     6061 taatggtttc acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa
     6121 tgtcgtgtcg tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc
     6181 taatcctaca gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta
     6241 catcccccta tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct
     6301 tgaacgataa atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta
     6361 ccacgctggt agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg
     6421 gccatcgcag taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg
     6481 ggaatacttt caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca
     6541 tctgctgctt ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca
     6601 taaaatggtg ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg
     6661 cctacgattg ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac
     6721 cggaaaatat tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt
     6781 ttgcgtccaa tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc
     6841 attgagatta atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg
     6901 atttgtttaa aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat
     6961 tcctactcct tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt
     7021 catcaagaca gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac
     7081 aacgttgaca tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt
     7141 tctttcgcta aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc
     7201 taatagttct gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac
     7261 ttcttttttc ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa
     7321 ctccatcaca gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa
     7381 gacgatggtc aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt
     7441 tgtttgcggg tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc
     7501 caatgctcgt gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc
     7561 ttgtccgcgt ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt
     7621 gctctttggt acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga
     7681 ccataacagg ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc
     7741 tttgtttttt aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag
     7801 ctcaagtcca ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat
     7861 tccataaata tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc
     7921 gtttttccca ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac
     7981 atctttctct tatgattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg
     8041 acgaaagctt gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct
     8101 cttccagtac ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg
     8161 tagtaagttt gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt
     8221 tacccatttt cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca
     8281 aagctttctc gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca
     8341 acttttttca agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt
     8401 tgaaacttaa gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac
     8461 cgattttatc attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg
     8521 ctttttccgc ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg
     8581 gcagattggc aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac
     8641 agtattcttt tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg
     8701 ccattttata tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg
     8761 tcctctcgta agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc
     8821 gcttttgaag ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc
     8881 ccgcttgtgt tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc
     8941 gttgaggcct tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg
     9001 tctaaccacg ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac
     9061 aattttctta cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata
     9121 tcaacccgat accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca
     9181 atgggatccc tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga
     9241 acagcagcat catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga
     9301 aacacacccg aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac
     9361 cgataaagcc gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca
     9421 catagatatt gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa
     9481 gtccttgggc cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat
     9541 caatacgtgc aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt
     9601 cagtcgtaac caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg
     9661 cgatgatgcg ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga
     9721 caagcatcgt tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga
     9781 tcaagagctt cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat
     9841 tatgtttagt tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg
     9901 gctggaaagt aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat
     9961 tctgatcata gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag
    10021 gagtcagttt ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc
    10081 caactaaata ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat
    10141 catggctaag tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta
    10201 aaatcacgcc ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc
    10261 atcccttttc ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa
    10321 gtctttccat agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat
    10381 gcagtacaaa agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca
    10441 attatctacc agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga
    10501 aggatctctt cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa
    10561 taaaaatatc gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta
    10621 gacaaaatcg agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc
    10681 acgatcatct tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa
    10741 acaccagagt ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact
    10801 agctggtctt ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata
    10861 aaaccaaaat cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt
    10921 tcgatctcaa aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa
    10981 agatagacag caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca
    11041 ttgagcaatt tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat
    11101 aataagtcgg cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg
    11161 atattcaatt cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac
    11221 agtacttctg ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat
    11281 aacttaaaca tggcaccact tccataactt tttatgataa ttactataaa aactatttga
    11341 tttaaatata caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa
    11401 ctagtaactt agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc
    11461 attttacaat gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt
    11521 atatatgaag aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt
    11581 agcttccctt ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg
    11641 cggtatctct atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca
    11701 cacgaaacaa acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc
    11761 actcatggtt atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt
    11821 catttggctg tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca
    11881 tgttatcaaa gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt
    11941 tggatttggg gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt
    12001 cttttggatt tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt
    12061 tttcgtaaga aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg
    12121 ttctctttgc ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt
    12181 agcattgttc gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt
    12241 gttcaagctt cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc
    12301 aacagctata tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt
    12361 ggatttctta aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc
    12421 tcattacgta cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa
    12481 aatcgcttaa taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga
    12541 acggccaaac aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg
    12601 caagcttttg aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata
    12661 agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag
    12721 aagctacttc tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga
    12781 cctcttgttc ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt
    12841 atttattgac gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa
    12901 aggattcagc ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct
    12961 tcagcaggct tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat
    13021 tgggtatttt attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat
    13081 ttgctgaaag caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc
    13141 aaacgattgc tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca
    13201 ctatcatcgg tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga
    13261 tgatggctgg aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca
    13321 tccatgcgaa agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg
    13381 tgatgatgtt gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa
    13441 aaagtgcact tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct
    13501 tacttgacaa ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc
    13561 tcgttgttgt tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg
    13621 aaacgccaac tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt
    13681 ttgtgatctt ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg
    13741 cacattttat cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag
    13801 actttgcaaa attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc
    13861 ttctccaaac agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg
    13921 aaaaaacagt tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg
    13981 cagatatcct tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc
    14041 catatatcct gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg
    14101 caatggattg gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc
    14161 aacggctact tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca
    14221 gacattttgc tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct
    14281 ttttcccgca aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc
    14341 tgattcgctt tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc
    14401 taaccataaa aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac
    14461 tggaacttcc tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta
    14521 aaagtttgat aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac
    14581 ctgttctttt acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa
    14641 tgtaaaccat atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg
    14701 tcccatgttt taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca
    14761 aaggtgccca tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag
    14821 cagcaatcaa gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa
    14881 attttgggaa agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga
    14941 ccgtgatgga tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga
    15001 tcactacttc tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg
    15061 cgatacgcag tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag
    15121 aaatggtcga tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc
    15181 tagaattaac agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa
    15241 cagaatatat ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct
    15301 ggaaattatt caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga
    15361 ttgcttcatt tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta
    15421 ttttgcgggc attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct
    15481 tcctgttcat tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga
    15541 aaatcttctt agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga
    15601 agccaaatga attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa
    15661 atgaaaaagg actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc
    15721 cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg
    15781 taaattcctt cttatttctt gaagctgacc acttctatca caacctcttt catatcttat
    15841 tacttcttca ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct
    15901 tgtgcatatt ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct
    15961 tttttatcaa gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt
    16021 ttaccttcga tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt
    16081 ggatcttttc ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca
    16141 cgtgtgtttt tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag
    16201 aaatcttgat attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt
    16261 tcgattccat tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct
    16321 ttttcccagc tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat
    16381 tgcttacctt taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg
    16441 attagtttct cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt
    16501 tgaccaagca agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg
    16561 gcgaaatcgg gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca
    16621 gtttcgcctt tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta
    16681 ccaaattcaa ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa
    16741 aaacgagaga caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt
    16801 gctctgtttt cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact
    16861 tttgtctgtt ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg
    16921 ctgactgctt gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg
    16981 tctgtacgcg gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat
    17041 gttttctttg cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca
    17101 taaggcaatg gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg
    17161 cttgtactga tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga
    17221 tttttttgaa gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt
    17281 tgtggacgga actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc
    17341 tgcacacggc ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc
    17401 aatcctacca gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat
    17461 tctttcgcag gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag
    17521 atccataacc gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca
    17581 agttcccctt ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga
    17641 gccagctgct tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt
    17701 tttttaggga tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct
    17761 tccggcattt tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct
    17821 tcataataat ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg
    17881 cttggttttt ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat
    17941 gggctgatga tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc
    18001 ttcacaccag cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt
    18061 gatatattca taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc
    18121 ttcgaaagac tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca
    18181 ccaaggctca ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc
    18241 agcttttcta gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa
    18301 aagggtcatt taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa
    18361 gtagcgataa agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct
    18421 tttgtaaagt atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc
    18481 ctgcttcgat gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg
    18541 atctgattcg tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg
    18601 atcagtaact catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag
    18661 atctgcagtg cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt
    18721 tcctgcagaa gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta
    18781 tacagcgagg gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga
    18841 acatccatct cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt
    18901 ctttttttca tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca
    18961 cgaaaaagaa agagaaaaaa gagactttca ttgataaact acatattgta gtttataata
    19021 aagagctgta gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga
    19081 aaaattattt aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac
    19141 atttatggct ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac
    19201 gttaggtaat tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac
    19261 tttcttggtg actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag
    19321 aaaattgatg ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg
    19381 cgtgactagt gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta
    19441 tatcagtaat gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt
    19501 tcttggaacc ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg
    19561 gatctctgct tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt
    19621 tttcgtcttc ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga
    19681 aaacatggtt tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg
    19741 tatgttcgtc acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag
    19801 tctgtacatt cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat
    19861 tgcttcgata cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga
    19921 caagattggg agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat
    19981 cccaatggca ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat
    20041 ctctgatgct tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca
    20101 agctgctgga cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg
    20161 gcctatgatc ggctccagtg tttccagtgc atttggttat cgtggcgtct ttctttcgac
    20221 ttctcttctt gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca
    20281 gcaagaaact gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc
    20341 tcatagacaa taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg
    20401 cagtgttttt gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc
    20461 caaggtttct tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact
    20521 tcctggtact aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc
    20581 ttcttcgatg gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac
    20641 tttcggttca ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt
    20701 gtttcgcgcc aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag
    20761 gactccgccg caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg
    20821 tccagtagct gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt
    20881 ggttgctata cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa
    20941 aggcgcatat acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat
    21001 tttttctaaa gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca
    21061 gatgattttt gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc
    21121 ttcttcttcc ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata
    21181 taattgctga taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta
    21241 taaagctaaa agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac
    21301 ggtttgataa agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata
    21361 gtccaatggt tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc
    21421 aacgagttcg tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt
    21481 cttttcccat ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc
    21541 ccccttatat cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac
    21601 aaatcagccc aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag
    21661 cgatccagta gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa
    21721 atcctgctaa aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt
    21781 gttttttgat tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac
    21841 aaatcaaaat tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta
    21901 agaaagtcaa taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg
    21961 catttttttt attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca
    22021 tcgttgtttc tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca
    22081 agccgatatt gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca
    22141 tagcaggttg tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg
    22201 aagcaaggat atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt
    22261 ctgaacgttt cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc
    22321 acatcataac catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc
    22381 cattcactag attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa
    22441 atgcgataaa tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga
    22501 ttgtattgat gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa
    22561 taaaaatact tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa
    22621 attctcccat ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca
    22681 accagtaacc ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac
    22741 cgaaaatacc accatctaga tctggacgct tttctcctaa atgattcagc gataatacca
    22801 aagctaaaat accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag
    22861 aactagccag atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca
    22921 gagcaaccag tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca
    22981 cttataaaat tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc
    23041 ttaacaaaaa gcaactttat tcatattatt ggatattatg aactttttac acaagtattt
    23101 caagtataaa atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag
    23161 aaaaggtgtt tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta
    23221 ttgtaaaact tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat
    23281 tccatcataa aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac
    23341 gacttctgca cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata
    23401 acggaatttc gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat
    23461 ttcacttgca tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata
    23521 taatgtgttt gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc
    23581 gccaccgatc cctagtcctt gacgttgacc aatcgtataa tatatcaagc cagcatgttg
    23641 tcctttgact tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct
    23701 caaaaattgt ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt
    23761 agcagtcgca aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc
    23821 tagagggaac atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg
    23881 atctttatta ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac
    23941 ttgcgcataa tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa
    24001 tgctttgaat ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg
    24061 gtattcggct aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga
    24121 gtaataagga atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc
    24181 cgtgcataca ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc
    24241 gtatccttgt tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc
    24301 tacgacaacg cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc
    24361 tgaaacatct acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt
    24421 ctgaaaattt catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca
    24481 gtacacaaac atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa
    24541 attggcatac tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt
    24601 gaaaaactat ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc
    24661 ttttcttttt agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa
    24721 gggaggcaaa agatacatga attactttat cagtgatatg cactttttcc atgaacgctt
    24781 attaggaaaa aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca
    24841 gttgatcact agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga
    24901 ccttgctatg catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca
    24961 actgcaagga acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta
    25021 tttagaaaaa aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt
    25081 aggtgccatc gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct
    25141 cgggcctaac gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat
    25201 cgagaacgat gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact
    25261 accatttggc actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa
    25321 gtacgatagg tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac
    25381 tcatattttt catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt
    25441 gtaacaagac ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc
    25501 ttttaccagt tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa
    25561 aacgattccg cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa
    25621 acgatcagct aatggtcctg caaataataa tccaattgga cctgccaggt tcatcaaaga
    25681 gttgaagttc cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc
    25741 catcagcagt gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa
    25801 gtacaagaac ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat
    25861 aatgatgtag gctataataa taggtttcat tcggtctttc catgttccta taaaactgat
    25921 cagtgcaccg cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc
    25981 aacggaacca ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa
    26041 caacagcatc acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa
    26101 ttgctgcagc ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg
    26161 agaaactttt gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac
    26221 atctaataaa ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg
    26281 cgcaatgatg aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg
    26341 agaagatgga acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc
    26401 gaccgaacgc ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt
    26461 tccgactact gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag
    26521 agctttttta tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg
    26581 aatcattcct agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa
    26641 ataccagatg atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga
    26701 taaaaagaac aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat
    26761 aaacatccct cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga
    26821 ttcttttttc aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt
    26881 acttagaaac aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat
    26941 aaaaaagaca actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt
    27001 caaaactagt tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct
    27061 aatcatccca tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt
    27121 gaaaatattg actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat
    27181 atcttttgat acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa
    27241 tggtcggatc aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt
    27301 atatcttttt gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt
    27361 tgtttcaaat gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt
    27421 atcctgattt ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag
    27481 acatctgttc atataagaat gacttcacta gttatttttt gacagcacga actaattgat
    27541 caaggaaata ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt
    27601 cttttagtat cggtgtatct tctccataca ttgcttctaa tacgtgtgaa ggctcgatat
    27661 ttcctgctgt acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca
    27721 gtagcaaatc atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg
    27781 ttaaatcacc atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt
    27841 gctggaatcc tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa
    27901 ctgcttttgc cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt
    27961 cctgctcccc gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac
    28021 cgacgccttt tggcccattg attttatgag cagagatact taataaatcg attcctaatt
    28081 cttctggatg gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg
    28141 gatgatccgc taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt
    28201 tcccgtacat cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga
    28261 tcgacagatt ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta
    28321 aatatttcat ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc
    28381 cttctttttg acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc
    28441 cactgttaaa gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg
    28501 ctgtttccaa tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa
    28561 acgtgtcttg catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg
    28621 ccgcatgatc cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca
    28681 cattctagca taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt
    28741 tcatgggcat agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct
    28801 cacttctgtc acaacttctt tccctctttt ttttgtttat tcgaattctt tttcttccag
    28861 acggaataac ggactcatcg gtgtattttc atggatgcgt ttgaccgctt cacccattaa
    28921 ttctgaacat gtaacgattg ttaagttttc tggatgacga ttttcttctg tgaaaacaga
    28981 atcagtgata caaatatctt tgattggtgc attgtctaaa atacttttcg catgatcgga
    29041 caataatcca tgtgacgcac aggcatatac ttcttttgcg ccgttttctt ttaatacatt
    29101 tgctgctcgt gcaagcgttg ctcctgtgtt caagatatca tctaccatga tacatgtctt
    29161 tccttgaaca tcaccgatca cataaccacc ctcagttgaa gcatcatctg catgatctac
    29221 gatggctaat gtgctgttca aatattctga aagactacgt gcacgctgaa caccgctgtt
    29281 ttttggcgaa acaactacga tgtcatctcc cgtcagacct aattgacggt aataatgagc
    29341 aaacaacggc atcgtaaata aattatctac aggaatatca aagaatcctt gtacttgaac
    29401 cgtgtgcaga tctaaagtca gtactcgagt tgcccctgct tcttcgatca agttagcgat
    29461 caatttcgca gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag catacccata
    29521 gtaaggcaat acgacattga tcgttttcgc acttgctcgc ttcattgcat cgatcatgat
    29581 cagtagttcc atatagtaat cattgacagg tgcattcgtt gattgaacga tatacacatg
    29641 atcccctcga atactttctt cgatattgat ggaaatctcg ccatcactaa attgcttcac
    29701 gacgctttta cctaattctg ttccgaacac tttcgcgatt ttttcagcta atggacggtt
    29761 gccattcaaa ctaaaaatct tcaacgtgtc atcctgatac ttattactca tgtagtcctc
    29821 cccaaaactt ctctattctt cgatagttta acatattttt ccaagatttt ctgttaattt
    29881 cagcaaattc ttaaggaagt gaaactgttc cgttactcca actggcagga atgactttat
    29941 gataatcgcc ccagttgtcg tgtactttgt aataacgttc tcctgtttca gtttcaaaat
    30001 aagcgtacgc tgtcacccaa tgattgccat atgtactgcc aagtactttc aaaataccaa
    30061 tcatcacagg tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag
    30121 ttgctcgtgc acgctcatga ttgccattct tctttaaaaa actagaaatc cctgtactaa
    30181 cctgaaaagg aatcgtagga agtcctagtg gttggatctg gctacgcaat gccttcgtca
    30241 aaaccaacga atcagccgta tttttcttgc gaatctcatt tggaagcatg caccccttac
    30301 gatagtcttg ataataggca aggagaactg ctgccgcgta agtaccacaa agatagcctg
    30361 agggggtagc ccatttctta tacctcaaaa aacgttcttc tggcaatccc acccagcaat
    30421 ttgcgagttc ctttcctgac cttttttctt cgatcaaaac gggaatatct tcttttctgt
    30481 tgatccatct agataactcg attggctctt cattgatcag cttccatttt ttacaagaaa
    30541 gaccctcttg ataagtatat tttgctggag aatattctac cggatgtcct gattgatcga
    30601 taatcacata actttgatct tcaaaaacca gagctaaaaa aagcaattta ctttttcttg
    30661 actgcagctg ctgagtgctg gctaattttt taattttttt atctactttg aaatataaag
    30721 gtttatccac tacttgattc agttgggatc gcctcctttt tttcattata agtgaaggat
    30781 tgaaaaaaac aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa
    30841 ttccttcatt ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa
    30901 caaagaacga ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc
    30961 aaaaatccct gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga
    31021 ccaacttttg atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga
    31081 aaaaatgaaa catttccttg catattatca actggcaaac gattatgacc gaagaaatga
    31141 gttgcatgct caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga
    31201 cgaattgaat aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct
    31261 ggatgtagat gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc
    31321 tttattcctt ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat
    31381 aggcgttttc tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca
    31441 ggcagaagca atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa
    31501 aagtgcagaa gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt
    31561 tattaaatac acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac
    31621 tccagataaa gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac
    31681 agaagctcat tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag
    31741 tggttatcta tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg
    31801 aacagacgaa gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga
    31861 tcaagtagtt ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt
    31921 agaacaaatg gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg
    31981 gcttggggat gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt
    32041 aggctacccc gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca
    32101 gggtggtact ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt
    32161 cagcaaatgg aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa
    32221 tgcttattat catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc
    32281 attttacagt ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc
    32341 tcatgagatt tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt
    32401 gaacaactgg tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat
    32461 cgaagaattc gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc
    32521 ggaaaatatt gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc
    32581 agatctttct atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa
    32641 aaaagaatac caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc
    32701 aaacgtgcaa ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga
    32761 cgctatgtat ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt
    32821 ggtcagcttc aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat
    32881 ttcggcttat ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa
    32941 gctgcttagt ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    33001 aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa
    33061 tcagcgtcct gacctgagta ttagagaaaa aaacggaaaa atagttcctc atattttgcc
    33121 gtttgttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gagattgtga
    33181 aaaagctgtc ctgcatcaag taataagaac agccaatcaa aagcagcaag gagaacctca
    33241 tctataaaaa gatagaagga agtgagacat ctattggtct catttccttc tatttttact
    33301 ttcctcagtc aattgtttta catcataaaa ggagaaattc cacttatttt tttcgctgtt
    33361 tcatgacata aacaactaca ccgattaaca aaattcctcc agctaatgct agcaacgggt
    33421 tattaaattc tcccgttttt gggagtcctt gagtgccatt tttttcactt tcttttgctg
    33481 tcctatccgg atgagaaggt tttccatttg gttttccatt tggtgattca tttcctggat
    33541 tttctctttc tggtttgtca tttcccgggg gcacatcggc agtttctcgc acagtgatcg
    33601 ctttttgctg ggtaacagat tctgtatgag cagaccattt ttccccatca aatatttccg
    33661 aacgataagt gacttctagc gtatattctc cttttgaagg tgctgaaaaa atagcggtat
    33721 gattagaaaa ttgagcactt gactgtgttt caacgatttg gatcttttct gggaaataac
    33781 gcatatcttc aaagacaggc gttccttgat ttggtgtgaa agttgacaca aaaactgtaa
    33841 aaggcttcag ttcaatgata tttccttctg cattctccgc cacaccattt gcttcttgta
    33901 taataatcgg attattttgt tcatttggtt ttgtctgatt tggttcttgt acgattgcat
    33961 actctggata ttccgaagac ggtgaggttt ctccgtcaaa aatagcacct acagataatg
    34021 gaagaccatc cactctttcc ttttctgttt gacgattccc cggtttaatc tgcatcagtc
    34081 catgaacatg gatacttcca ttttcatagc gcgtaacagg ctgctgacct tctttcgaac
    34141 atgtatttag agactcgatc caatcttttg atatggtatg tccaagagta ttttctgatt
    34201 gtttctctcg ttcattccaa tagtagttca acgagtggta gatttgatct tcgttttgat
    34261 tcaatgggcg gatctgctct ctcatctcaa gattctctgt tcggaaagat aagataccag
    34321 aagcatggta gtctgtgttt cctgctgaag aggtataaag agccacatta tatgaaccgt
    34381 tattaaaact tgtactgcta ttgacgataa tatctgttcc gctattggaa tcgattcctt
    34441 tcgcaagatt ttcgaaagac aaactattga tcaattcatg agggcccgtt aaagaagaac
    34501 cgcccatttt gaatccatta ccgttacctt ctccctcaat tcctggtacc cagccgtttc
    34561 gataagcaac tgagtttttg atgattactt ttccgatcga acctgtttca tttttagcaa
    34621 aaaggtccca accgtcatct gcattatgat acgcgataca accgtcaaat acatttcctt
    34681 ctcctattgt cagtttggcc gcaaatccat cggcatcttc aaatcctgga tcggcatttt
    34741 caaaagatgt acagttcttg actaagttat gcgcgggcca tgcttcgaaa ggatcatttg
    34801 agctaccgct gatttggatt cctgtatttc cattccgata agcatgaatc tcttcgatca
    34861 aattatgatt tccagaaatc tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa
    34921 accctcgtaa accccagtag tgactgaaca gtgtcatccc atttccagtt cctttaaaat
    34981 caagtacagg acgcttgttt tctggatttt tctctgcaat caactgaatc gggttttctc
    35041 ttgtcccgtt aacgcctttt ggaatcgtca atcctctttc gaaggaataa gttccaggcg
    35101 ccatgacgat tgtctgccca gcatatgcgt atctcaatgc ttgtgtcaga gataatggat
    35161 tttgtctgct tccgtttcct gcatctgacc cttctggtgt tacataaact gtcgaatatt
    35221 taggtttacg gtagtccact gtatgaagaa tctcttttgt ttcataacta gtcatctcta
    35281 cgtattcttc tggcggatag tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc
    35341 cttccatcag ttttgtctga taatcaaatt cccgattcgc agtgatagcc aagttttttg
    35401 cgttcttaag cacctctcct gttgctttat ttttaattgt caataatccg tcagcatttg
    35461 ctctaaagga catagtatga tcttggtttc ctgaatgatt actagaggtc acagtgtaaa
    35521 tcggatcaat caagcgagta ggtctttctt gcccaggagg atctgtcgca ggatctgagt
    35581 aagcaacttc gattccttct accttgatcg tcatattcct tgctgcagca aatcctgcat
    35641 aaataaccga ttcatctgaa gcgtataatt tttcccagtc atacatgatg tcagaagcca
    35701 tttcttctcc tgtttcagga tcgtaataat atgcctctaa accagtattc gtttttttca
    35761 tctctacgtc cagtttagta atcgagttcg tggccggaaa cagattccca tgaccgtttc
    35821 cgaatatatt ataagaacct gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg
    35881 ttgtttccac tgagacactc ccaggagctg gcgcagcttg cggatcttca gatgatattc
    35941 ctgtgattgc ccgtgtcccc aatccaagtc gcatcgtata tttgctgccg ctcgtgttga
    36001 ccacttcttg tctatccgag tcccagacat attcgatccg actgcctagt atggcaaatg
    36061 aatttgaata aaatgcctgc ccaaacaaat gattttttgg tactgcgtct cttaccatta
    36121 aagcaaatcc ttcttgggca ttcgtatagg tccaagattc tatttcgatc gtcgcccgca
    36181 tcctaaagtt gatgtctttg gggattgctt gataataata ggtcattcca tctgatccgc
    36241 ttgactggaa ttttccgccg ttattcgtgg aagagagccg cacgccgtct tctaatggtt
    36301 caatggcata cctactagca cttgtgcttt ctccgaaaac aacccctttc cactcttcat
    36361 ttgcttcttc agattgcttt tttgtctttt cattttctga ttctttcatc ataggatcat
    36421 ttccttcttc cgtatccata tcttcactag ttcttggtat ttcctccatt gttgacatcc
    36481 cttcaatgtt cacgtgtgca ttttcttcgt tcacagactg ttgttcgtta gcagcaactt
    36541 caacgacaga aattaaaacc ggggtactga gcataaggag attcatcatt agcgcactgt
    36601 attttatacg tttttttaac aagtgtattc ctcccgtaac aaattagaaa gcgtttactt
    36661 aaagtattac ctaatttatt tttactgtca ataaaaaaca aaattataat aagtgtattt
    36721 tataaaaaga aaaaatataa cttaagttgt tgataataaa gagatttatt aacgtaaaaa
    36781 gaataagttt ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat
    36841 gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa
    36901 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
    36961 aacttgccac ggcatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
    37021 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
    37081 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
    37141 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
    37201 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
    37261 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
    37321 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
    37381 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
    37441 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
    37501 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
    37561 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
    37621 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
    37681 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
    37741 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
    37801 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
    37861 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
    37921 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
    37981 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
    38041 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
    38101 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
    38161 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
    38221 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
    38281 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
    38341 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
    38401 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
    38461 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
    38521 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
    38581 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
    38641 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
    38701 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
    38761 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
    38821 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
    38881 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
    38941 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
    39001 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
    39061 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
    39121 ttctctaaat atagaaaatc cttctttagt ccgatactct ttatagtagt ccgtatgcta
    39181 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
    39241 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
    39301 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
    39361 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
    39421 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
    39481 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
    39541 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
    39601 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
    39661 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
    39721 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
    39781 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
    39841 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
    39901 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
    39961 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
    40021 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
    40081 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
    40141 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
    40201 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
    40261 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
    40321 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
    40381 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
    40441 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
    40501 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
    40561 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
    40621 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
    40681 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
    40741 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
    40801 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
    40861 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
    40921 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
    40981 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
    41041 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
    41101 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
    41161 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
    41221 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
    41281 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
    41341 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
    41401 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
    41461 gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
    41521 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
    41581 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
    41641 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
    41701 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
    41761 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
    41821 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
    41881 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
    41941 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
    42001 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
    42061 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
    42121 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
    42181 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
    42241 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
    42301 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
    42361 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
    42421 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
    42481 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
    42541 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
    42601 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
    42661 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
    42721 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
    42781 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
    42841 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
    42901 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
    42961 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
    43021 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
    43081 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
    43141 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
    43201 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
    43261 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
    43321 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
    43381 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
    43441 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
    43501 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
    43561 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
    43621 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
    43681 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
    43741 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
    43801 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
    43861 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
    43921 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
    43981 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
    44041 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
    44101 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
    44161 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
    44221 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
    44281 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
    44341 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
    44401 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
    44461 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
    44521 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
    44581 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
    44641 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
    44701 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
    44761 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
    44821 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
    44881 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
    44941 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
    45001 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
    45061 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
    45121 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
    45181 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
    45241 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
    45301 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
    45361 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
    45421 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
    45481 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
    45541 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
    45601 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
    45661 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
    45721 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
    45781 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
    45841 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
    45901 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
    45961 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
    46021 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
    46081 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
    46141 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
    46201 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
    46261 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
    46321 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
    46381 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
    46441 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
    46501 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
    46561 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
    46621 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
    46681 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
    46741 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
    46801 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
    46861 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    46921 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    46981 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    47041 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    47101 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    47161 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    47221 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    47281 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    47341 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    47401 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    47461 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    47521 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    47581 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    47641 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    47701 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    47761 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    47821 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    47881 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    47941 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    48001 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    48061 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    48121 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    48181 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    48241 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    48301 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    48361 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    48421 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    48481 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    48541 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    48601 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    48661 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    48721 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    48781 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    48841 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    48901 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    48961 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    49021 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    49081 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    49141 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    49201 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    49261 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    49321 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    49381 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    49441 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    49501 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    49561 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    49621 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    49681 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    49741 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    49801 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    49861 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    49921 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    49981 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    50041 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    50101 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    50161 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    50221 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    50281 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    50341 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    50401 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    50461 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    50521 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    50581 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    50641 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    50701 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    50761 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    50821 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    50881 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    50941 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    51001 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    51061 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    51121 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    51181 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    51241 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    51301 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    51361 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    51421 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    51481 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    51541 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    51601 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    51661 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    51721 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    51781 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    51841 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    51901 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    51961 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    52021 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    52081 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    52141 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    52201 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    52261 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    52321 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    52381 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    52441 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    52501 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    52561 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    52621 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    52681 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    52741 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    52801 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    52861 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    52921 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    52981 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    53041 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    53101 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    53161 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    53221 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    53281 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    53341 acttcatcta ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg
    53401 gggatctttt ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt
    53461 ccgacatacc attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca
    53521 taggaggata gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca
    53581 tcgattgcta aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc
    53641 cattcaaggg tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct
    53701 aagaatacct catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata
    53761 tgatagactg actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc
    53821 ttcaatttta accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca
    53881 acaatcaaca aaacaatgcc catctaatta taatttaatt taattttaat tttaaatcaa
    53941 atgatatcct ttttagttaa aaacaccaat tatacaacat ttattattaa aattaaatta
    54001 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
    54061 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
    54121 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
    54181 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
    54241 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
    54301 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
    54361 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
    54421 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
    54481 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
    54541 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
    54601 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
    54661 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
    54721 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
    54781 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
    54841 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
    54901 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
    54961 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
    55021 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
    55081 gaaccgagct ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga
    55141 cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg
    55201 ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc
    55261 gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt
    55321 gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat
    55381 aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt
    55441 tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg
    55501 aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa
    55561 tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata
    55621 agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca
    55681 ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg
    55741 ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag
    55801 gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca
    55861 ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa
    55921 tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa
    55981 cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa
    56041 tgattcctat tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac
    56101 tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg
    56161 tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat
    56221 agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat gcagattata
    56281 aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg
    56341 aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat
    56401 taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga
    56461 ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc
    56521 agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca
    56581 agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga
    56641 aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa
    56701 agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac
    56761 actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga
    56821 tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt
    56881 catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc
    56941 tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc
    57001 ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac
    57061 agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg
    57121 ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta
    57181 atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt
    57241 cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat
    57301 tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta
    57361 cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt
    57421 atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt
    57481 ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt
    57541 aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac
    57601 cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt
    57661 cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt
    57721 tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc
    57781 ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg
    57841 ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt
    57901 taaataactt tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag
    57961 gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt
    58021 tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat
    58081 ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag
    58141 tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat
    58201 tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc
    58261 agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac
    58321 tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca
    58381 gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc
    58441 cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct
    58501 aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt
    58561 ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt
    58621 tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc
    58681 gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta
    58741 cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat
    58801 tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga
    58861 gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat
    58921 tatctctgga atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga
    58981 cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga
    59041 ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga
    59101 aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta
    59161 tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt
    59221 agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa
    59281 cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg
    59341 cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga
    59401 accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag
    59461 aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat
    59521 aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc
    59581 ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc
    59641 acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta
    59701 cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg
    59761 tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc
    59821 caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat
    59881 tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc
    59941 tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg
    60001 tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg
    60061 cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg
    60121 caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa
    60181 taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat
    60241 cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata
    60301 gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa
    60361 attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt
    60421 tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa
    60481 tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta
    60541 tacataacct tgatacttcg accacttata tatcgaacac acaaatacct atccattgca
    60601 ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt
    60661 actcttagta aagtatctga actattaatt tttaaactat catttttatc ctcaactaca
    60721 tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga
    60781 attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat
    60841 tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt
    60901 aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat
    60961 ataccctata aaagctaata agatggttag gaatccttta gaatttttac tcattcatag
    61021 acacttcctt aaataacttt t
//
LOCUS       DACDCJ010000006        59559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDCJ010000006 DACDCJ010000000
VERSION     DACDCJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.006"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IV038_000386"
     CDS             161..391
                     /locus_tag="IV038_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP8340636.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IV038_000387"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IV038_000387"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8340637.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IV038_000388"
     CDS             1802..1978
                     /locus_tag="IV038_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8340638.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IV038_000389"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IV038_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP8340639.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2594..3061
                     /locus_tag="IV038_000390"
     CDS             2594..3061
                     /locus_tag="IV038_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340640.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="IV038_000391"
     CDS             3146..4030
                     /locus_tag="IV038_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8340641.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4147..4380
                     /locus_tag="IV038_000392"
     CDS             4147..4380
                     /locus_tag="IV038_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP8340642.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="IV038_000393"
     tRNA            4468..4548
                     /locus_tag="IV038_000393"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="IV038_000394"
     tRNA            4555..4626
                     /locus_tag="IV038_000394"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
     gene            5141..5848
                     /locus_tag="IV038_000395"
     CDS             5141..5848
                     /locus_tag="IV038_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8340643.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IV038_000396"
     CDS             complement(5907..6551)
                     /locus_tag="IV038_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP8340644.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="IV038_000397"
     CDS             6677..6964
                     /locus_tag="IV038_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340645.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="IV038_000398"
     CDS             7072..7392
                     /locus_tag="IV038_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8340646.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="IV038_000399"
     CDS             7473..8786
                     /locus_tag="IV038_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8340647.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8783..9502
                     /locus_tag="IV038_000400"
     CDS             8783..9502
                     /locus_tag="IV038_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP8340648.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="IV038_000401"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="IV038_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP8340649.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10403..12937
                     /gene="secA"
                     /locus_tag="IV038_000402"
     CDS             10403..12937
                     /gene="secA"
                     /locus_tag="IV038_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP8340650.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13063..14164
                     /gene="prfB"
                     /locus_tag="IV038_000403"
     CDS             join(13063..13125,13127..14164)
                     /gene="prfB"
                     /locus_tag="IV038_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP8340651.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14269..14955
                     /gene="ftsE"
                     /locus_tag="IV038_000404"
     CDS             14269..14955
                     /gene="ftsE"
                     /locus_tag="IV038_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP8340652.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14939..15832
                     /locus_tag="IV038_000405"
     CDS             14939..15832
                     /locus_tag="IV038_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340653.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15984..17432
                     /gene="cls"
                     /locus_tag="IV038_000406"
     CDS             15984..17432
                     /gene="cls"
                     /locus_tag="IV038_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8340654.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17769..18626
                     /locus_tag="IV038_000407"
     CDS             17769..18626
                     /locus_tag="IV038_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP8340655.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18741..19661
                     /gene="pstC"
                     /locus_tag="IV038_000408"
     CDS             18741..19661
                     /gene="pstC"
                     /locus_tag="IV038_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP8340656.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19661..20545
                     /gene="pstA"
                     /locus_tag="IV038_000409"
     CDS             19661..20545
                     /gene="pstA"
                     /locus_tag="IV038_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP8340657.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20560..21366
                     /gene="pstB"
                     /locus_tag="IV038_000410"
     CDS             20560..21366
                     /gene="pstB"
                     /locus_tag="IV038_000410"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8340658.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21382..22140
                     /locus_tag="IV038_000411"
     CDS             21382..22140
                     /locus_tag="IV038_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP8340659.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22153..22830
                     /gene="phoU"
                     /locus_tag="IV038_000412"
     CDS             22153..22830
                     /gene="phoU"
                     /locus_tag="IV038_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP8340660.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23067..24098
                     /locus_tag="IV038_000413"
     CDS             23067..24098
                     /locus_tag="IV038_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8340661.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24095..24862
                     /locus_tag="IV038_000414"
     CDS             24095..24862
                     /locus_tag="IV038_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP8340662.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24962..26530
                     /gene="liaX"
                     /locus_tag="IV038_000415"
     CDS             24962..26530
                     /gene="liaX"
                     /locus_tag="IV038_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP8340663.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26830..27147
                     /locus_tag="IV038_000416"
     CDS             26830..27147
                     /locus_tag="IV038_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP8340664.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27147..27497
                     /locus_tag="IV038_000417"
     CDS             27147..27497
                     /locus_tag="IV038_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP8340665.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27664..28602
                     /locus_tag="IV038_000418"
     CDS             27664..28602
                     /locus_tag="IV038_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP8340666.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28617..29447
                     /locus_tag="IV038_000419"
     CDS             28617..29447
                     /locus_tag="IV038_000419"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP8340667.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29491..30516
                     /locus_tag="IV038_000420"
     CDS             29491..30516
                     /locus_tag="IV038_000420"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8340668.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30531..31469
                     /gene="galU"
                     /locus_tag="IV038_000421"
     CDS             30531..31469
                     /gene="galU"
                     /locus_tag="IV038_000421"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8340669.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31594..32034
                     /locus_tag="IV038_000422"
     CDS             31594..32034
                     /locus_tag="IV038_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP8340670.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32037..32639
                     /locus_tag="IV038_000423"
     CDS             32037..32639
                     /locus_tag="IV038_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8340671.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32698..33804)
                     /locus_tag="IV038_000424"
     CDS             complement(32698..33804)
                     /locus_tag="IV038_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8340672.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34014..35015
                     /gene="ccpA"
                     /locus_tag="IV038_000425"
     CDS             34014..35015
                     /gene="ccpA"
                     /locus_tag="IV038_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP8340673.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35352..37697)
                     /locus_tag="IV038_000426"
     CDS             complement(35352..37697)
                     /locus_tag="IV038_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8340674.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37976..38878
                     /locus_tag="IV038_000427"
     CDS             37976..38878
                     /locus_tag="IV038_000427"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP8340675.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39091..40866
                     /locus_tag="IV038_000428"
     CDS             39091..40866
                     /locus_tag="IV038_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP8340676.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40932..42518)
                     /locus_tag="IV038_000429"
     CDS             complement(40932..42518)
                     /locus_tag="IV038_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP8340677.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42769..42993
                     /locus_tag="IV038_000430"
     CDS             42769..42993
                     /locus_tag="IV038_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP8340678.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43135..44886
                     /locus_tag="IV038_000431"
     CDS             43135..44886
                     /locus_tag="IV038_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8340679.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44886..46670
                     /locus_tag="IV038_000432"
     CDS             44886..46670
                     /locus_tag="IV038_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340680.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46996..47847
                     /locus_tag="IV038_000433"
     CDS             46996..47847
                     /locus_tag="IV038_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8340681.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47901..49652
                     /locus_tag="IV038_000434"
     CDS             47901..49652
                     /locus_tag="IV038_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8340682.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49656..49826
                     /locus_tag="IV038_000435"
     CDS             49656..49826
                     /locus_tag="IV038_000435"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340683.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49866..50768)
                     /locus_tag="IV038_000436"
     CDS             complement(49866..50768)
                     /locus_tag="IV038_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8340684.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50999..52402
                     /locus_tag="IV038_000437"
     CDS             50999..52402
                     /locus_tag="IV038_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8340685.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52402..53835
                     /gene="ascB"
                     /locus_tag="IV038_000438"
     CDS             52402..53835
                     /gene="ascB"
                     /locus_tag="IV038_000438"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8340686.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53980..54906
                     /gene="trxB"
                     /locus_tag="IV038_000439"
     CDS             53980..54906
                     /gene="trxB"
                     /locus_tag="IV038_000439"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP8340687.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55120..56847
                     /locus_tag="IV038_000440"
     CDS             55120..56847
                     /locus_tag="IV038_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP8340688.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56919..57212
                     /locus_tag="IV038_000441"
     CDS             56919..57212
                     /locus_tag="IV038_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8340689.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57304..58164)
                     /locus_tag="IV038_000442"
     CDS             complement(57304..58164)
                     /locus_tag="IV038_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP8340690.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58256..58684
                     /locus_tag="IV038_000443"
     CDS             58256..58684
                     /locus_tag="IV038_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8340691.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58846..59022
                     /locus_tag="IV038_000444"
     CDS             58846..59022
                     /locus_tag="IV038_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP8340692.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59049..59495
                     /locus_tag="IV038_000445"
     CDS             59049..59495
                     /locus_tag="IV038_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP8340693.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        19395 a        10331 c        12766 g        17067 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgacgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttaatgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtatttcagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgataaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggcgc gctaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattccg attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gggtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaaggtt gtgaaaaata cggctagcac
     2521 tgagcgataa agaaaaagag gaaatttatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag taaccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag tgattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaaatttc aattcaagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caacttccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gcgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttacggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggggttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatatt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atggaccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4681 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4741 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4801 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4861 ccgggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4921 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4981 ctaggttgga catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     5041 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     5101 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5161 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5221 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5281 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5341 tacatatgtt gcacgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5401 aattatggaa tcacaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5461 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5521 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5581 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5641 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5701 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5761 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5821 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc gtattagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agaccgcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtatgcccgc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttcg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcgatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tcttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa ttgctgtagt atgcgataat aaagacaaag
     6661 gcaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tctgatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagacgtg gatgtgttgt tattaggccc acaagttcgc
     7261 tttatgaaag ctgattttga gaaacgtctg gaaccaaaag gcattccttt agatgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg agcaactgga ttctttgtca tcgctgtgga atgagaacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc tcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggagatcaa gcagacgttt gacatccaca aacttgcttt acgctttcat cggcgtttgc
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatatt
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagata ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaattct atccccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcagatc gcacagccga aaatcatccg
     9481 aactatttct ttggcgagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcaaatgctg
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttgcaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaagtt tttttgagtt tcctcctctt
    10261 ttagatgaaa tatgaataaa ttatgaagtt cttactttaa tacgcttaag atatttcaat
    10321 tatctataac taatgataaa atgaaaagta aagtctacat agtctggata atacacagaa
    10381 ggaaaaggag attacacaac gcatggccaa tttgattcgt tcacttatcg aaaacgataa
    10441 gaaagaacta aaacgtttag atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat
    10501 ggcagcatta actgatgaac aattacaggc aaaaactgct gaattcaagg gcagatacca
    10561 aaaaggagaa acattagatc aattacttcc ggaagctttt gcagtcgtcc gagaagcagc
    10621 aaaacgtgta ttaggtttat atccatacca cgtacaattg atgggtggaa tcgtgcttca
    10681 cgatggaaat atccctgaaa tgcgtactgg tgaaggtaaa accttgaccg caacgatgcc
    10741 agtatatcta aatgctctga ctggcgaagg ggtacacgta gttaccgtca atgaatattt
    10801 agcaacccgt gactcgacgg aaatgggcga actttataat ttcttaggat taacagtggg
    10861 gttgaatatc aactcaaaat cttcggatga aaaacgcgag gcttataatt gtgatatcac
    10921 ttatagtacg aataatgaat taggatttga ctacttgaga gacaatatgg tcgtttaccg
    10981 ccatcaaatg gtacaacgtc cgctgaatta tgccattgtc gatgaagttg actcaatctt
    11041 gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa gcagaaaaat ccacagcttt
    11101 atatacaaga gcagacaact tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga
    11161 tgtacaatca aaaacaatca gtttaaccga agcagggatc gaaaaagcag aagaaacatt
    11221 cggtttggat aacttatacg acattgaaaa tactgcattg acccatcact tggatcaagc
    11281 attgagagcc aactatatca tgcttttaga tatcgattat gttgtccaag acggaaaagt
    11341 actgattgtt gaccagttca ctggtcggat catggatgga cgccgttatt cagatggact
    11401 tcaccaagca atcgaagcta aagaaggcgt agagatcgaa gacgaaacga aaaccatggc
    11461 gacgatcact ttccagaact atttccgtat gtacaagaaa cttgcgggaa tgactggtac
    11521 agctaaaaca gaagaagaag aattcagaga aatttataac atccaagtat accagattcc
    11581 gacaaatcgt ccggttatcc gtgatgatcg tccagatttg ctttacccaa cattacaaag
    11641 caagttccac gctgtcgtgc aagatatcaa agatcgctac cgcaaaggtc aacctgtgtt
    11701 agtcggaact gttgcggttg aaacatctga gttattatca gatatgctga atcaagaacg
    11761 tattcctcat gaagtgctga atgcgaaaaa tcactttaaa gaagcagaga tcatcatgaa
    11821 tgctggtcaa aaaggtgctg tgacgattgc taccaacatg gctggtcggg gaacagatat
    11881 caagttagga cttggggtaa gagaacttgg tggtctagca gttatcggga cagaacgaca
    11941 tgaatctcgt cgtatcgata accagcttcg aggacgtgca ggacgtcaag gagaccctgg
    12001 tatgtcacag ttctacttgt cattggaaga tgatttgatg aaacgattcg gttcagaacg
    12061 aatcaaagct ttcttagatc ggatgaagat cggtgaggaa gatgctgtta tccaaagcaa
    12121 gatgctgacg aaacaagtag aatctgcaca aaaacgtgta gaaggaaata actatgacac
    12181 acgtaaaaac gtcttacaat acgatgatgt gatgcgtgaa caacgtgaag taatctataa
    12241 acagcgacaa gaagtaatca tggaagaaaa aagcttatcc aagccactaa tgaacatggt
    12301 caaacggaca atcagtcgtg tagtagatgc gcatacacaa ttagagaaag aaaattggaa
    12361 tttggaaagt atcatcgatt ttgccggcag tactcttgtc catgaagata gtattactct
    12421 tgcagatatc gaaggaaaaa cagctcagga gatcaaagag tatctaaacg aacgagcaca
    12481 agaagtattt aataccaaag cggctcaact aaatggtcca gagcaactgt tggaatttga
    12541 aaaagtcgtg atcttgcgtg ttgtggactc taaatggaca gaccatattg acgcaatgga
    12601 tcaattacgt cagtcaatcg gcttacgtgc ttatggacag aacaatccat tagtcgaata
    12661 tcaaacagaa ggctataaga tgtttgaaga tatggtaggc gccatcgagt atgaagtcac
    12721 tcgaatcttt atgaaagctg agatccgtca aaacgtacag cgtgaacagg tttctcaagg
    12781 aacagcttct cactcagaag acggagatgc caaagaaaat agtaatacta gcgcgaaaaa
    12841 acagccaatc catgtgaaca aggtcggaag aaatgatcct tgtccttgtg gaagcgggaa
    12901 aaaatataag aactgtcatg gaaaaaatgc aaactaattt ctgacagcag gattggtgag
    12961 acaaattctt tttttaagaa ttcgtctcac cttctttctg ttatttgctg attctgttag
    13021 actagatata atgaaaaaag tatagtgaaa gaaggaaaaa acatggaaat atccgaaatt
    13081 cgaaatctcc tgacagaaat gcaggaaaag gtcacaagtt tcaggaggtc tctttgactt
    13141 agatcagctg gaagaagaca tcgcggaagc agaagcaaaa atggctgaac ctggtttttg
    13201 ggatgacagc gaagccgcac aaaaagtgat cagtgaaaat aacgctaata aagaaaccta
    13261 tgaccaattc cattctcttg ccgaagaact agaagaaatc gaattgatgg tggagatgct
    13321 gcaagaagaa cctgatgcag caatgcaaga agaagcagaa acacgtatca atgcattaga
    13381 tgaaaaaatg aagacgtatg aattatctat gctgttagac ggaccttatg atcgaaacaa
    13441 tgcgatcgtc gaattgcatc caggggctgg cggaacggag tcccaagact ggggcagcat
    13501 gcttttacgt atgtatacac gttgggcaga gcagcatggc tatcaagtag aaacactaga
    13561 ttatcaagca ggtgatgaag cagggatcaa gagtgtgacc ctgctgatta aaggacataa
    13621 cgcttttggt tatctaaaat cagaaaaagg tgttcatcgc cttgtacgga tttcgccttt
    13681 tgactcagcg aaacgacgtc atacttcttt ttgttcggta gatgtgatgc cagagcttga
    13741 tgaaacgatc gatatagaaa tcaatccaga tgatttgaaa atcgatacgt accgagcaag
    13801 tggtgcaggt ggtcagcaca tcaataaaac agaatctgct gtacggatca ctcacttgcc
    13861 tacaggaaca gtagtagcta gtcaggcaca acgctcacaa ttgaaaaacc gtgagcaagc
    13921 gatggggatg ttaaaagcta agctttatca attagaagtg gaaaagaaag aacaagaagc
    13981 agctgcactt cgaggagaac aaaaagaaat cggctggggt tcacagatcc gttcttatgt
    14041 tttccatccc tattctatgg taaaagacca tcggacaaac gtagagacag gaaatgtcca
    14101 agcagttatg gacggtgacc ttgatttatt tattgacggc tatttaaaaa tgcatctaca
    14161 ataagaaccc attgttaaat tgaaataaaa ttgtaagatt ttgcaagcat gttgaaacat
    14221 tgccatgcta taatagctaa agactgaaaa agatatggag agaacgccat gattgaaatg
    14281 aaggatgtaa tgaagaagta ctcaaatggt acgaccgcta tccgcaatct ctcgattgtc
    14341 atcgaacaag gagagtttgt ctatgtggtt ggtccgtctg gagccggtaa atctacattc
    14401 atcaaattga tgtatcgaga agaaaaagct tcaaaaggaa ctttgaacgt cgcaggtcat
    14461 gatctaatag caatcaaaaa ccgcgaagtt ccttatctac gcagagaaat cggcgtggtg
    14521 ttccaagact ataaattatt accaagaaaa acagtatatg aaaatgttgc gtacgctatg
    14581 caagttatcg gcagaaaacc tcgagatatc aaaaaacgag taatggaagt gctggacctt
    14641 gttggtttga aacacaaagt tcgagtcttt cctagcgaat tatccggtgg ggaacagcaa
    14701 cgtgtctcta ttgctcgggc aatcgtgaat acgcctaaag tacttatcgc ggatgaaccg
    14761 acaggaaatc tggatccaga aaactcatgg gaaatcatga aattattaga tcggatcaat
    14821 gcccaaggaa ctactgtcgt gatggctaca cataacagta cgatcgtaaa tacgattcgg
    14881 caccgagtaa ttgctatcga aaacggccgc attattcgag atcaagcgga aggggaatat
    14941 ggatacgatg attagaacct ttttccgcca tttattagaa agtatcaaga gtttgaaacg
    15001 taacggctgg atgacagttg cttcagtcag tgcggtaacg atcacgttga ctttagtcgg
    15061 tgtattcatg gcagttatca tgaatgcaac taaattagct caggatatag aaggaaacgt
    15121 tgatgtatct gtattcgtag atatcggtac aaaagacgaa gagatgaaaa agctagaaaa
    15181 agaattgaat gacttagatc atgttaaaaa agttgaattt tcaagtaaag aacaggaatt
    15241 gaaaaaaatc caaaacgaaa tgggtgattc ctggaaattg tttgaaggtg acaataaccc
    15301 gttatatgat gtgtatgtag taagtgctac tgatccttct tatacaaaac aagtagcaga
    15361 agaagcagca gatttgaaga atgtttctcg ttctgattac ggtggagctt cctcagatcg
    15421 gattttccaa atcgctggtg cagttcggac atggggatta gttgcagcgg cattgctgct
    15481 gttcgtagcg atgtttctca tttcgaacac gattcggatc acgattttgt ctagacagcg
    15541 tgaaattcaa atcatgcgtt tagtcggtgc aaaaaatgga tatattcgct ggccgttttt
    15601 ccttgaaggt ggctggatcg gtctgatcgg tgcgattctt ccgattttga tcatcacatt
    15661 tggttatgca tggtttttcc aattgatcaa tccatcatta ttacgttccc attattcatt
    15721 gattcatcca caaaatatcg tttggaaaat caatctttta atggtcggca ttggtgtgat
    15781 cattggttca cttggctcga tcatttctat gagacgtttc ttgaaattct aataccacaa
    15841 tataaataaa atgagaaagt tggacagttt cgttccagct ttcttttttt attggctagg
    15901 agaatagaag gaagcggctt ttttcccttt actttaaaaa acaacttggg tattcttaag
    15961 ttggtagagg aagaggtgaa gccgtgaaaa ttatcgttga caattttttt acgatccttt
    16021 tgattatgaa tattttgctt tcctttatca tcgtttttcg agaaagaaag gaaactgctc
    16081 aaacatgggc atggctgctc gttttgatgt tcattccagt tgtaggattt attttatata
    16141 tttttttagg aagagggatt tccaaagaga aaatctttga tttgaagatc caagggaaaa
    16201 tcggtaagaa cctggaaatt gaagaagaca aacaggcatt gatgcgaggg ctttatcctc
    16261 atccgcctac tggacaagta gatgtgaaac aattgatcta tatgctcacg gtatttgaaa
    16321 gtacgctcta tacgactaaa aatgaaatca ctttatttac agatggtcgt gaaaaattcg
    16381 atgcgctgat tgaagatatc aagcaagcag aagaccatat ccacttccag tattatattt
    16441 atcgcagtga tgcattaggt gaagaagttc gagatgcatt gacagaggcg gcaagacgag
    16501 gggtaaaagt cagagtcctt cttgatgcat ggggttccac acaagtttct tccagctttt
    16561 ttcagaattt gaaaaaagca ggcggagaaa tcgcattttt ctttcctcta tttgttccgt
    16621 atattaatcc tcggatcaat tatcgcaatc atcgtaaaat cgtagttatt gatgggaaaa
    16681 tcggatatac aggtgggttc aatgttggaa atgaatatct aggattggta aagaaatttg
    16741 gttattggcg agacaatcat ttgcgtattt acggagaagc ggtgtatatc ttgcaaaacc
    16801 gttttttgat ggactggaat tctcagcata caaaagaagt aggctattct cccaaatttt
    16861 ttccgtccat tcactctact ggtgatatag ctgttcaaat cgtgacaagc ggaccagata
    16921 ccgaacatga acaaatcaaa atgacttatt tgaaaatgat ttcgttagcc aaacgggaga
    16981 tattgatcca aacgccttat tacataccgg atggatcgat tcatgaagca ctgaagttgg
    17041 ctcttctttc aggcgtaaaa gttcatatcc aaatacctaa caaaccagat catcttctcg
    17101 tttactgggc aacgtattct ttcgctgcag agctgatcga gtatggagca aggatcgaaa
    17161 catatgagaa tggatttatt catgcaaaga cgatgattat cgatggtggc attgtttcag
    17221 ttggctcagc aaatatcgat gttcgttcat ttcgcttgga ctttgaggtc aatacactga
    17281 tttatgatga acgaatggct tcacgtgtaa gaaaagcatt ttttgaagac tcgaaaattt
    17341 ctacgcatct cacaaaagaa atgtatgaaa accgaggaat aatcatcaag atgaaagaag
    17401 gattagcaag attgatttcg ccgctccttt aaaacacaaa aaggacatga gaaccatcaa
    17461 cagatggaga tctcgtgtcc ttttctaacg gggccatttc tgagagtacg tcagaaatgg
    17521 agaaagcgtc tcgcatgagc gaaattaact ttggggtatc tcttgttttt tggggaaagc
    17581 aagaaataag ctaatatatg ctattaaaag agacacattt caaagccagt atagcataga
    17641 aacttttaat gagatagttt tttttacaat tatgaggatt tattttgtta tttaatgatt
    17701 ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag gattaaagga
    17761 gatttctaat gaaaaaactt aaattattct taccaataat aggattagct attcttttat
    17821 caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc tcttctgctt
    17881 tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct ggaaaattca
    17941 tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca ggagcagtcg
    18001 acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc tcagcattag
    18061 tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat attggcgtaa
    18121 aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa aactggagtg
    18181 aagtcggtgg aaaagatcag ccagtagtta tattgaaccg tgcttcagga agcggaaccc
    18241 gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca caagaacaag
    18301 attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc agctatgttg
    18361 ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta gaaccgacag
    18421 atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg tacacaagag
    18481 atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat gaagtacaag
    18541 ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc gaacgtgatt
    18601 ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt agctaaactt
    18661 ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt atgttaaacc
    18721 tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca aaatcaaaac
    18781 gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg ctgatcgtgt
    18841 tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc ttttttactg
    18901 ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc caaatcggag
    18961 caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt attgtgacct
    19021 ttttatctgc tattgttgcc acaccatttg ccattggtgc agcagtattc atgacggaga
    19081 tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt gtaggtattc
    19141 cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc agaagtattt
    19201 ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt atgatcttgc
    19261 cgactgtcac aacgatgact gttgatgcat taaaggcagt accacggcac tatcgggaag
    19321 cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg ctacgagcag
    19381 ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt ggtgaagcct
    19441 tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt actactcctg
    19501 cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg ggaacagttg
    19561 aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta ttctttaata
    19621 tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa aacgctcaga
    19681 taaaattgct acaggtgttt tatatgtagt ttcggggatc atcgtattga ttttagcagc
    19741 tttactactt tatatcttag ttcggggaat accgcacatt tcatggtcct tcttgaccga
    19801 accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta actcgattta
    19861 tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg gtatctatct
    19921 ttcagaatat gctggaaaaa actggctgac agatgtgatt cgcacgtcga ttgaaatctt
    19981 gagctcattg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg ttattcaatt
    20041 ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca acttgcctct
    20101 tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac gagaagcagg
    20161 attggcttta ggtttgtcac gctgggaaac agtgataaaa gtggtggttt cagaagcgac
    20221 accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg aagcagctgc
    20281 attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact ggaatccatt
    20341 aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg tccatatttg
    20401 gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg gcgcttctgc
    20461 cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg gcaaacgatt
    20521 ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata taactggaat
    20581 gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac ggatgatcta
    20641 catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt tgaaaaaaat
    20701 aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct tcgttcgttg
    20761 aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat gtatcaaggc
    20821 gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat tggcatggtt
    20881 tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt tgctttgaaa
    20941 cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag cttgaaacaa
    21001 gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc tttatctggc
    21061 gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga tatcttgctg
    21121 ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga agaaacactt
    21181 gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca acaagcggct
    21241 cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata tgacaaaact
    21301 aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc cggtcatttt
    21361 ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga tcttcatctg
    21421 tattatggaa agaaagaagc actaaaaggg atcgatctaa gtttcaatca aggggaaatc
    21481 actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc tttgaaccgg
    21541 atgaatgacc tgattccgaa tgtaacgatc accgggagtg ttttatataa agacaaagac
    21601 atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat ggtattccag
    21661 cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt gaaattaaaa
    21721 ggggaaaaaa ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa ggcagcgtct
    21781 gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc cggtgggcag
    21841 cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat cttattggat
    21901 gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat gctgttagaa
    21961 ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc ttcacgtatt
    22021 tcagataaga cggcattctt tttagatggt cacttgatcg aatacaatga tacgaagaag
    22081 atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg atttggataa
    22141 ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct ccataatcag
    22201 ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg tgcatacatc
    22261 aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat caataacatg
    22321 gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc agttacaact
    22381 gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg tatggccgat
    22441 catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag aataccggaa
    22501 atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga caacgtccta
    22561 gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat ggatcaaaga
    22621 gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca ggaaaatcct
    22681 gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga acggatcgga
    22741 gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa aatcactgag
    22801 ttaaatacga accacaatga gattttctaa actaaagcgg tgattctgcc gtttgtttag
    22861 gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag taataagaac
    22921 agtcaaacaa aaatagctcc tcatattttt catttggctg gacttattac cgcagatgca
    22981 agcttttgaa cactgtttat tcgggaaagg gtttgtgaca aaatatgtca caaacccttt
    23041 tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt
    23101 ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat gaagtacctg
    23161 tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca ggaagactga
    23221 tgaacacgaa tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa
    23281 tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat
    23341 ggcagctagt agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg
    23401 gcgccatgtt gcttattggc ggaaaacatg gtctatttgc tttaggaagt ctcatcatca
    23461 atatcgtttt acttttactt ttgttgctca tctttactaa aatagaaggg atttctctat
    23521 taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg
    23581 gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat
    23641 tttttatttg tcttttgaca atgaatcttt ggaaagacca aggactgcgc tatgaggaac
    23701 tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag
    23761 ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc
    23821 gacaaccaga aataacagcg aaacagttat ggctttcggg aaggcaagtg ggtcgagatg
    23881 tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc
    23941 ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat
    24001 tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg
    24061 tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg
    24121 gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg
    24181 cttattcatc aatttaggga tctttttctt gttgttattc ggttatcatc agaagtggcc
    24241 gatactggca ttgtcggtca taggattttt attgattgct gtggttatct tattttttat
    24301 caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg
    24361 tttcctacta ttgcttccta tcactgattt tttagcaatc caaggtttta caacgataga
    24421 actagaagaa ttatctggct tagataagac tttagcgatt gattttagat tacttacgag
    24481 atcgttgctt ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag
    24541 tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc
    24601 tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt
    24661 catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca
    24721 agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc
    24781 cgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa
    24841 agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc
    24901 agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc
    24961 catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga
    25021 aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa tcaaaaaaga
    25081 agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa
    25141 acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca
    25201 agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc
    25261 ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa
    25321 acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga
    25381 attagcacag cgtcagacgt tagaagcaga aatcaaagag ttagaagcta gtggcgatac
    25441 actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg
    25501 gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca
    25561 agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg
    25621 aaaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg
    25681 gaaagatgtc agcttgcgtg ttcctgggat tgcgacaacg aaatttgagc atgaatttta
    25741 ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt
    25801 aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat
    25861 gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta atgaagatca
    25921 tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa
    25981 acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt
    26041 ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac
    26101 tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat
    26161 cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa
    26221 cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa
    26281 aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg
    26341 tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga
    26401 agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga
    26461 tggtgaaatc gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac
    26521 agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt
    26581 aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta tctcttgaac
    26641 accgtttacc aagagattat gaacttgcca attatcgccg agtcacaagg aataatttca
    26701 aaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcaaaaaga
    26761 caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg
    26821 tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt gacagggact
    26881 ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta
    26941 ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt agctttgatt
    27001 atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa
    27061 aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc
    27121 gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt
    27181 taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga
    27241 cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga
    27301 cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg
    27361 cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat
    27421 tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac
    27481 ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga
    27541 atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta
    27601 gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca
    27661 tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg
    27721 acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga
    27781 ctagagttga ctggttattt taattattat tcccataatc gtttacagct atttggcagt
    27841 aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga
    27901 ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa
    27961 atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct
    28021 cgcttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc
    28081 cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata
    28141 ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat cgcagacgat
    28201 cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg
    28261 gaacatctga ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg
    28321 agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa
    28381 gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtaggc
    28441 attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta
    28501 gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa
    28561 agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt
    28621 tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat
    28681 tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag gctgttcggg
    28741 taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta
    28801 ttggcgctcg cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc
    28861 aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga
    28921 tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg
    28981 ctgcaccaag tgtattatta gcacaagcaa tcggtcgttg gggaaatttc atgaaccacg
    29041 aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca
    29101 tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac
    29161 tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga
    29221 aaaaaggtga agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg
    29281 aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat
    29341 ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt
    29401 taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt
    29461 aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca
    29521 ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt
    29581 tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat
    29641 ttaccagatc attgtctacc cgattccatc caagcattca ccaatatgga agatgcagta
    29701 aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa
    29761 gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa
    29821 caggggacac ataaaaggat ctctgatatc ttgttagagg aaatcccagt agaaaaaaga
    29881 aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa acatgacatc
    29941 actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc
    30001 atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca
    30061 gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat
    30121 aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca
    30181 atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct agtcgttact
    30241 tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc
    30301 ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca
    30361 gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac aatctatgaa
    30421 gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatggg
    30481 aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtca
    30541 aaaaagcagt tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta
    30601 tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag
    30661 cgttgaaatc aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg
    30721 aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg
    30781 aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc
    30841 atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat
    30901 ttgtcgttat gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattaa
    30961 tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt cctcatgatg
    31021 aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg ttatacaatg
    31081 tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg
    31141 gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa ccaggagccg
    31201 gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa cgcgtatttg
    31261 cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt atgaaaacaa
    31321 gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag tacatcctgt
    31381 ctttaggtaa aacattagaa gcagaaggaa gcaagatcga tcataatcca aaaggaataa
    31441 cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc agctgctaat
    31501 tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct
    31561 tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg
    31621 atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt gttgcaagtt
    31681 tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac gatcgaaatc
    31741 gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttactggt caaaagtaat
    31801 gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt
    31861 gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat cgcgacgaaa
    31921 gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt cggtgggaca
    31981 gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataaaaatgg
    32041 ctaaaaaagg tggattttta ttaggtgcag tagtcggagg cacagccgca gcaatcgccg
    32101 cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct gcccaagcgg
    32161 atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa tcgactgagt
    32221 taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt gatctagcag
    32281 gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa tctgacgata
    32341 tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa aaaacggcag
    32401 aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact tcagaagata
    32461 tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat gaaggaatgt
    32521 cagaagcaaa accagttgca gatgactcaa aagaaatgat gaaaatggca aaagaagatg
    32581 cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca aatcactaaa
    32641 atcacaaatt tatagaataa aaaggtggga gcggactagc tctcaccttt tttactctta
    32701 tttttctaaa acaagtaatt cttttggtgt agtcgtgaat ggcacacagc catcgcttgt
    32761 cacatataca cagtcttcga tacgtacccc tactttttca ggaatataga ttcctggttc
    32821 aagagagaag cacatccctt cttcgattac taagtcattt ccttctacaa gggaagggaa
    32881 ttcgtggacg gttgttccga tcccgtgacc taaacggtga ttgaaatatt cgccgtagcc
    32941 atagctgttg atcacgtttc gtgcgatttg atcaagttca cctgctgtca cacctggacg
    33001 gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg ttgtagattt tttcttggaa
    33061 gtcggttggt ttttgataag caattgtacg agtagcatca ctgcaatagc cattccaaac
    33121 aacgccaaga tcgaataaca caagttcatt cggtgaaatc ttcgtatttc ctggtgtacc
    33181 atgagggctt gcagcatttg ctcctgcaag gactaatgtg tcgaaagaca tttgtgaaac
    33241 accttgtttt ttcaattggt attcaatttc tgcaatgatc gcttgttcag tcacgccttc
    33301 tttcactgct ttaaagccaa tctcaaatgc aacatcggcc catttaccag cttcgatcag
    33361 tttgtctttt tcctctagag ttttgatcag ttgtagacgt tggatgatgg aagttagatc
    33421 agtgtaagaa tccgttcccg tcaagatatc agagatagca tcgaagcgct cgacagtcaa
    33481 ggcgttcttt tcaatcccta cattgccagg ctgaccatag cgagcacgaa gttcttttcc
    33541 gatgatctcc caagggtttt cgctgtctaa atagccacgg acatcatacg cccaactact
    33601 gttttgagca tcttcaactt ccaaagcagg agtgaaaagg aaaggatctt gttcaagagg
    33661 gataaaaaga gctagtacac gctcatgagg atcactttcg tatccggaaa agtaagcgat
    33721 gtggccaggg tcgctgatat aagccaattc gacttggtgt tgtgccatcc attgacgtag
    33781 ttcattgatt tttacgttat tcatttggca acctactttc taaattaatt ttgtattcaa
    33841 taattgtacc actgatttat taaaagacca acgttctaaa aactgaaaaa agtaaaaatt
    33901 ttcatgaaaa cagagataaa acggttgcaa ttcttgagat tatttgatat tctattttag
    33961 aaatgtaaga cgtattttca gaaaacatat aaaatatgaa taggagaaca caaatggaaa
    34021 agcaaacaat cactatctat gatgtagcga gagaggcaaa tgtttcgatg gctactgttt
    34081 cacgtgtcgt aaatggcaat ccaaacgtta aaccagcaac aagaaaaaaa gtcttggaag
    34141 ttatcgatcg cttggattat cgtccaaacg cagttgcgcg tggcttggca agtaagaaaa
    34201 caacaacagt tggtgtaatc atccctgatg tcagcaatat gttctttgct tctttagcac
    34261 gtggtatcga tgatgtggca acaatgtaca aatacaatat tatcttagca aattcagatg
    34321 gcaacgacca aaaagaagtc aatgtattga ataacttact ggctaagcaa gtggacggcg
    34381 tgatcttcat gggtcaccat atcacagacg aaatccgtgg agaattttca cgttcaaaaa
    34441 ctcctgttgt attagctggc tctattgatc cagatgaaca agtaggtagc gtaaacattg
    34501 attatgtatc cgcaacaaaa gatgcagtaa acaaattggc aaaaagcggc aataaaaaaa
    34561 tcgcttttgt cagtggtgcg ttgatcgatc ccatcaatgg acaaaatcgt ctgaaaggct
    34621 ataaagaagc attaagcgaa aataacctac catatagcga aggattgatc tttgaagcac
    34681 aatacaactt caaagatggt ctatctctag tcgatcggat ccataatagc ggtgcaacag
    34741 ctgtctatgt ttcagatgat gaattagcca tcggtatttt ggatggttta ttagaccgtg
    34801 gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa caattcacta ctgacagaag
    34861 tagctcgtcc tcgtttaaca agtatcacac aaccattata cgatttaggg gctgtatcta
    34921 tgcgcctatt gacgaaaatg atgaataaag aagaagtcga agaaaaaacg attatcttgc
    34981 cttatggaat cgaagaaaaa ggatctacaa aataataaaa ggacaaccat tcagaggttg
    35041 tgtgcctgcc gtttaactct gcgttacaag gagcaatttc aaaaaacagc atctcatgtt
    35101 tgatgaatat tgtgactcgt gattcggagt tggcaggaga acaccgttta tttgggaaaa
    35161 ggggttatga caaaattttg ttataatcct ttttttgtgt cttaaaaaag agagaaagtg
    35221 gttgaaatta catttatcgt atataaaaca atccgtgtat agatgctgca ttttgttcac
    35281 caccatcaaa aaaataaggg actgtgacaa tactattgtc acaatccctt attactgtgt
    35341 attctattaa atcaatcatc tttctcatct gattctttag ttgcttgatt cgctcttgca
    35401 taagctgaag cggtgttcca gtaatctgta tagttttcat cggtaccgcc aacgccaaat
    35461 ttaaaggcgc ttttcttcgg tccatttttc gcccatagcg attcaactgt ttttcctggt
    35521 gtttggatgg attttttatt gacagtaact tttccaggtg tttgaccagt aaatccagat
    35581 acttttttct tcatcacatc agatgataat tcgaatttct catcagtgcc aaaaatgtca
    35641 ggttttgctt ggtaagcttg attgatcaga ttggcaagat aattagccga acgtatcctt
    35701 gctttgctgt ccattccagt attgtcatcg tgccctgccc agctactgat ggtaatagac
    35761 ggagtagaga caattagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
    35821 caatctgctt ttcctagatt tccgtttaag cttgagatgg catctttgaa tggtgtagtg
    35881 atttttgcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttccgaa
    35941 tagatccgga cagagttgct ttcgtgttta taaattacat ttcctgcgtt atctgtgatg
    36001 gaatcgatga tatagccttc ttcatagacg ccgccgtttg ctaaagcctg aaatccgttg
    36061 gtctgttgaa gcgtcgtgac atctactgta cctaaaggag cagattcaac gccccaattg
    36121 tcatttgccg ggtagttcat ctttgccaag tagtgctcgt aagcatattg tttcgatcca
    36181 ccggaattca acacatcttg atataagtga taagccggga tattgttgga ccattctagt
    36241 gactcacgga cggtttggaa ggtgttcgat ccttcgttag tagcattgac gattttctcg
    36301 ccagcatttt ctccttcttg ccaagttgtt gcataatctg atacacggga ttctgatccg
    36361 ataagcccca tgtcgattgc aggtccataa acaagaactg gtttgattga tgagccagct
    36421 tgacgttctg tatcgaaggc atggttattt tgattctgac tgtaattccg tccgccaacg
    36481 aatccgtaga tgcggccagt tctgttatcc atcaatacat ttccggtttc tacttgactg
    36541 ccagcaccat catctaacat gtatccataa ttttcaacgc cattttgcaa tgcagcatag
    36601 acatctttat cgattgtcga atggattgta tatcctttat tttggatcgt ttgctgtgcc
    36661 ttttgataat aagcgtcact gaccgatgaa tcagacatat cagcgttgtc tttttcagcc
    36721 aattgttgtg caatgattct tgttgcttca ttcatcacag tataatacaa gaattctctg
    36781 tcagtctgtt ctgcgatttg ttgtggtaga aagtcttttg taaggtcata ggcttttgct
    36841 tcttcgtatt gttctttcgt aatttggcct tcacgataca tactgaataa aacaaaatct
    36901 tttctggcaa ggccggcaga aagatcttct tttagcgcgc ctgtgttcgt gtatggacta
    36961 tacgtgatcg ggctttgggg taatccagcg atataagcag cttgcggcag cgtcacatct
    37021 tttgcatcta caccaaagat tccttttgca gcttcctgga ctccagcaat gttttgtccc
    37081 ttattattac gaccaaatgg tgagacattc aaatacgtag caatgatctc atcttttgaa
    37141 aagtattttt ctacttgtgc agaaagaatg atctcgtttg ctttacgttt gaatgttgtt
    37201 tcatctgtaa ggatctgctg tttgactaat tgctgtgtca aagttgatcc accagaagag
    37261 ccaataccag tagcttcaga tactaatgct cgtaaaacag ctttaggaac gtaaccttgg
    37321 tgtttatcga agtattcatc ctctgtgctg ataattgcag ttttcaataa aggtgaaatc
    37381 ttatcagatg aaatagtcgt gcgcatcaga tctgtctgga tttttgatat ctctgtatta
    37441 tctgcataaa caatctttga cgtttctgta atgtttccta gttctgtctg cagtgctttt
    37501 ttagtcggca attgtgtgtc ttcgacgaga taggcaaaat agccagcgcc tattccaata
    37561 cctagagctg cgaacatcaa aagaagaaca gtaataaaaa cagtcaatga ttgaaaaact
    37621 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
    37681 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
    37741 aataatagaa caaaaaaaca gacaaggctg tttttggcag gaatttcttt tttaaaagag
    37801 ttaaaacgtg agaaaatgat ctttattata aaagagttct taagcttttc tttagagaaa
    37861 atttcagtta ctatggagaa cagggcaaag aaaaatcaat cattttcttt gtatatcatc
    37921 ttaccaatat ataggaattg ttagtaacaa ttgcctttca aggaggaatc catccatgtt
    37981 attaggctca cacgtcagca tgagcggaaa gaaaatgctg ttaggagcag cagaagaagc
    38041 agccagttat ggatcttcga cttttatgat ctatacaggc gcccctcaaa acactagaag
    38101 aaaagccatc gaagagatga atattgatgc cggaaaagcg tacatggcag aaaaaggtct
    38161 aacaaatatc gtcgttcatg ccccttatat cattaatttg gggaatacga tcaaaccaga
    38221 aatgtttcct tttgccgtcc aatttctgag agaagagatt gttcgtgcgg aagctttagg
    38281 cgctactcaa attacacttc acccaggtgc tcatgtaggg gcaggaacag aagccggaat
    38341 tgctagaatt attgaaggct tgaatgaagt tttgacaaaa gaacagacgg cacaaatcgc
    38401 tttggagaca atggcaggta aaggtacgga aatcggtcga acatttgagg aattagcgca
    38461 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgaca cctgtcatac
    38521 gaatgatgcc ggttataata ttaaagaaga cttttcaggt gtaatggaag agtttgataa
    38581 gatcatcggg ttagatcggc ttaaagtact ccatatcaat gattctaaaa atccgcaagg
    38641 ttcgcataaa gaccggcacg ccaatattgg ttttggaaca atcggattcg acacgctgaa
    38701 taagatcgtc catgatccaa tgtttgacaa tatttcgaaa atcttggaaa ctccctatgt
    38761 aggcaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatcgcca tgttcaaagc
    38821 gcaaacattc gatccagaag tgtttcctga gttattagca gaagaagtga tactctaaga
    38881 ttttctatct gacagcagca aaaaaggacc aggcacattg ccttgtcctt ttttgtttaa
    38941 gaaacaaagg agtacttttt gcagtctttt tcatcatgca tcttctcccc cctcttttta
    39001 aatttagaca aacaaggtat actggtagaa aagtaaaaaa acgaaaagtt tcacaagcaa
    39061 gtggcaaaac gtgattggaa ggacgaaaag atgctgaaca ctttaaaacc cttgatcaac
    39121 caactaaaaa agaaacatac atactttatt cttggcatag ccctattatt tacaattatt
    39181 cgtttgtatt tatatagtaa agcaatctat ggaacagaca tgtcggcaac ctatgatgac
    39241 cagctgctga ttcgctatgc tcaaaatatt ttggacggta aatggctagg ggagtatacg
    39301 actacaacac tttccaaagg aatctcctac agcttatttt tagtacttgc acataaactg
    39361 catctttctt atgcagtttt gttcggcatg ttcagtattt ttgccagtat attggttgca
    39421 ctcgcgttta aaccgattgt gaagaacaag tatcttcttt taagtatcta tttttatttt
    39481 ttgttttctc cgattagttt tacacatgaa tattccactc gaacatatcg taatgccctc
    39541 gtgattccgg ctgttgagat cattatcgct tgtttattag ctatttatta ccgcaaatac
    39601 ggttctagca gaaaattgct gccatggttt ttagggcttt ctgtcgcttt tcctttcttt
    39661 tggttcattc gcgaagattc tatctggctt cttcctttta ctgtagtagc tttgggtatc
    39721 agtttcgtcc agatcttgct agggcaagac ttttctttca aagaagtaaa gaagttaaaa
    39781 aaacgactct ttcttctctc gaaacgggaa tggctgaaac tttcattgtt tttacttcct
    39841 atagttggct tttccatcgt ctatcaatgg atcgaatata aaaatgagac aacatacggt
    39901 attgcaacca tcaatgaccg ttccagtgga gaatttggac gtttgatgaa acaactgatc
    39961 cgaatagatg atgaaacgaa cttgaataaa gaaaacagtg agatatgggt ctcacatgct
    40021 gcactaaata aagcattagc cgcatctcct acattccata ctgtttctac ccgtattgac
    40081 gaattatata cgactcacgc gtggactcaa ggtggaaaag taaaagaatt acccggagat
    40141 atcatctttt gggcattgcg agatgtggta agcgaatctg gttattacca aaacaatgca
    40201 aaagaaacca atgatttttg gaaaaaagtc aaccaagaat tagaagaagc ctatcacaag
    40261 ggaacaatca aaaagaaaaa agagttctat ctgatgggat caggagatgg aaagacgatg
    40321 agtgatctgc caatcgtatt cgatttcctt caatcaggat acgcttatgc atggcgatat
    40381 aaagatttcc atcaaggagg aaattatagt ttcggaacgc aagaacaagt caaacaggct
    40441 caagaattat taaaccttcc gctaatggag aattggatcg atagtaatga accgagaaca
    40501 cctaatttcc aattaacaaa atcagcaaaa gtcgcgaatg tcatcataaa aatttaccaa
    40561 gttctggcta ttcccgtgct gattgcttca atagcaggga tattacttat gatactagga
    40621 accgtatttg caaaagctga tcgtgcctat tatagttcct ctttgattgt ggtcaccgga
    40681 ttaatactga cacaattggt ttttcttttc ggtgtctcat ggttttgttc ttatgcgcca
    40741 gaaaaaaagg actatttttt aagtgtctac actggtgcag gcgtgccatt aacgcaattc
    40801 atcgtagtaa caagcatgct agttttcagt aaattaaatc tgaaaaaagc tttatcttct
    40861 aaataaaaag acccttggta gctagatttt taacctaact atcaagggtc tttgtgtgga
    40921 aaaatcagtt tttattcgat ttcttctacc gtttcctgca aaagctgttc atattttttg
    40981 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
    41041 acataagcga tgtgatcagc caatgctagt tgagcggaag tggcttgctc gccgaagaaa
    41101 ctttcgatct gtcctggtaa tcctcgtaag ttcaagtaag ttagactaat cacagagaac
    41161 cagccaagaa gttttacgat gaatgaattt ttaaatcggc tacccatctc cgcttggctg
    41221 ttggtcatca tcagtaaagg aatcatcgaa aacggcaacg caaaagccaa gaacacctgg
    41281 gattcattca ttaagttatt caaagcggca tgttcttcga tcgttccttt tgtacttgta
    41341 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgaccag tcttcttagc
    41401 caaatcggga tacgcatatg gatgaatcct tccatgatga cttgaccggt caacgtacca
    41461 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt ggacaagata
    41521 ccagattttg ctacagcaat caaaatacca ttgctcatag ctgatggatc agataaagct
    41581 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaagac agcgacaccc
    41641 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
    41701 gtagaaaaac gtacagcttg tgctacatct tcttcgtttt ggtgatcgat cttccgagtt
    41761 tgtgaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
    41821 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtatgactg
    41881 ttagggataa agccagcaaa taaactgtgc caatctgggt tagacaagcc aacttgatag
    41941 acaaaaacga ataaaatgac gaaaattaag cagacaacca gtgcttcgat cttacgaaac
    42001 ccgacttttg tcagtagtaa gagaacgaaa acatctaata cagtaataaa gacggcaatc
    42061 accaatggaa tatcaaaaag taagtagagg gcgattgctg caccaatgac ttctgcaata
    42121 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacctaa tgtcttactt
    42181 gtgcgtgctc ggattgcctg tgccaaatcc atttggctca cgattcctaa ttttgctgcc
    42241 atatactgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
    42301 tggaaatttt gtcctccggt gatcgaagta gaccagttac ccgggtccat atacccaact
    42361 gctactaacg caccaggtcc agagtaagca agaagtgttt taaaaaaact tttccccttt
    42421 ggtacatcaa ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
    42481 cgatgcttat gaaattgttt attggattga ttattcaaga aaaaaccacc tttcctattt
    42541 atctacgatt acattctaca cctcaaaaag aaaaaattaa agaaaaagat tcggtacgcc
    42601 taattttttt tcatggcgcg tcttgctaga tgacgaaaga aattttgttc tcctctaaaa
    42661 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
    42721 taaaataagt cattgagagc aattcggaaa tagaaaggag agatttatat gttatcaaca
    42781 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
    42841 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
    42901 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
    42961 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaacgact ttttacgggc
    43021 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagat tattcagcaa
    43081 aaaagacaag cacagacaat cctaggctct tgataaagac aaaggaggag taaaatgtcg
    43141 atttttagaa aattaggctg gttttttcgt caggaaaaga aacattattt aattggggtt
    43201 ttctcgctat tcgcagtggc cttggtacag ttagttcctc caaaagtgat tgggatcatc
    43261 attgatgaga tcgccgataa aaatttatca ataaagatca ttttcgggtg gatcttccta
    43321 ttgattatcg ctgctttttt acaatatctt tttcgttata tctggcggac gaatatttgg
    43381 ggaagtgcag ctcgattgga gaaagaactt cggcagcagt tattcaaaca ttttacagaa
    43441 atggacaatc tcttttacca aaaatatcgg actggggatt tgatggctca cgcaacgaat
    43501 gatttgaatg cgatccagaa tgtagcagga gctggtatct tgacctttgc agattcagtg
    43561 attacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
    43621 attgcgctga ttcctctgcc tttgctggcc gtcacttcac gagtgctggg gtcaaaactc
    43681 catgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
    43741 agtatcactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctagcggat
    43801 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
    43861 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
    43921 ggtggaaagt atgtcttgga aggcacgatc tcgatcgggc agatggtctc atttattagt
    43981 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
    44041 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
    44101 gaacaactgg gtgcagtgca gaccccggca aaaggcgagc tgaatgtagc tatttcctct
    44161 tttaattatc cgaaagaaga atcaccagca ttagaacaaa tcaagtttgc gatccaagaa
    44221 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacgat catcaaatta
    44281 ctgatgcgtg aatacgatga ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa
    44341 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcagga tcacttcttg
    44401 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
    44461 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
    44521 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
    44581 tcgattgctc gagcattgat caaaaatcct gaattattaa tattagatga tgcactctct
    44641 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
    44701 aaaacaacta tcattgcagc tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt
    44761 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcactaggc
    44821 ggctggtatg aacaaatgtg ggagaaacag caattagaag cgaagataga aggaggcgag
    44881 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
    44941 aaaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
    45001 ctatcttatt tgcgtttgct ttgtctgtga tcaatgtcct attgccaagg atcatccaaa
    45061 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
    45121 ctggtatgta cttatttgga gtcatagtaa aaagtatcat ttggtttttc caatggtatt
    45181 tgtattccat ggcctcattg aagacctatc agtatattag ggtaaaatta tttgaaaagc
    45241 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcaacc gtttctcgag
    45301 tgacgaatga cacagaaacg ctatttgaat tttggtacgt ctttttgatg gtattgactg
    45361 ggatctttgc agtcgtttct tcattccttg ccatgtttca aatcaatgga gaaattgcct
    45421 tgtggacttt ggtgtttttg ccgattttag gaatcgtcat ctggtattac cagaaattca
    45481 gttctcgtat ttaccgaaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
    45541 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
    45601 aatttgaaca agtgaacaat gagtatttag acacgcgtta tgcgatgatc aaaacgaatt
    45661 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggtgttga
    45721 ctttgtttgg gatcgatgcg ttgcattctc ccgtagaagt tggactgatc tatgcattta
    45781 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcatct
    45841 tcacagatgg gatggttgct ggtagtcgta tcttgaagat tatggatacg gaagaatatg
    45901 caccacaaca aaaccctgga gcagatcaaa tgattacttc aggtaagatt gagtttcgca
    45961 atgtaagctt ttcttatgat ggagagcaca atgtattaaa caatatctct tttgttgcta
    46021 atccgggaga gaccgttgca ttagtgggac acaccggaag cggaaaaagc tcgatcatca
    46081 acgtattgat gcggttttac gaattttatg aaggtcagat attgattgat gatcatgaca
    46141 ttcgagaata tccaatggaa gagttacgta agaaaatggg gctggtcttg caagatgctt
    46201 tcttgtttta tggggatatc gcaggaaata ttcggatgat gaatccagct atcacagacg
    46261 aacagatacg ccatgcggct gagtttgttc aagcaaacaa attcatcgat gaattaccaa
    46321 aaggatatca ctcaaaagtg atcgaacatg gtgccagcta ttctagtgga caacgacagc
    46381 tgatcacttt tgcgcgaaca atcgtgactg atcctaaaat tctagtattg gatgaagcaa
    46441 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattggca aaaatgcgtc
    46501 aaggacggac aacgattgcg attgcccatc ggctgtcaac gattcgagat gcagaattga
    46561 ttttagtttt agacaaaggt catatagtag aacgcgggaa tcatgaaaca cttctagcgc
    46621 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtatataa aagatgttgt
    46681 gacagtagaa ttttgaccta agcactagag tgaaaataaa acatagcttc tcatattttt
    46741 catctgtgag agttagtgtc gtagtcacca gatttaaaaa aagattcgac agctagtcag
    46801 aagtgcctaa tagccactaa gaaagcgttg acaaaagcat ttgctttcga taatataaag
    46861 aaaacgatac aacttggata gtgattgcct gatgcaagct aaacctgatg agtctcccat
    46921 ggtttcatgg acggcttttt gggtttttct ttttgttttg gatacagccc tatacatcat
    46981 ggaaaggagc aaaacatgct gattgagaaa gtactgaata acaacgtcgt aataaccagg
    47041 gatgaaagaa atcaggaaat gatcgtgatg ggaaaaggtt tggcattcaa aaagaaagtg
    47101 ggtgacccga tttttgactc atgcatcgac aaaaaatttc agttgacaaa tgacgggctt
    47161 tcacaacagt tccaagaatt ggcacaagcg attcctcttg agtacttaga aatttcttgt
    47221 gaaatcattg aatatacaaa agaggctcta caagtccagt tgaatgattc tgtgtatatt
    47281 gcattgacgg atcatttgta tacggctatc gaacgtcaca aaatgggaat atttgtacca
    47341 aatgtattgc tgtatgatat caaacaacta tttcctaaag agtatgccat cgggaaaaaa
    47401 acagtgaaga aaatctcgga taaataccac atcaagttat cagaagatga agccggattt
    47461 atcgcattgc atttagtcaa tgcacaaggc attcaaagcc agcaagacaa tacctacctg
    47521 tttactataa caatacagga tattctaaat atcgtccgct attactataa gattacattt
    47581 gatgaagatt cagtccattt ttttcgattc gtcacacact tacgtttttt tctgacacgc
    47641 gtcctagatg gagaaaaatc agaagtggga gaagtttctg aggaattgtt tcaggttatt
    47701 caaaaaaaat ataaggatac gtttggttgc gttgagaaaa tagcagactt tttagccgag
    47761 acgttcaact accagatgtc cacggacgaa aaaatgtatt taacgatcca tactgcgcgt
    47821 ttagtgcatg agaatcgttg taaataaaaa aaggaggata aattaacaag aacgtatgaa
    47881 ataagggtag tgacattcag ttggctaaac cttccactaa taaattggag ggaatagaaa
    47941 tgggaaaata ccaagaatta gcgagaaaaa tcgtagaaaa tgtcggcggg aaggaaaata
    48001 tcaatggttt gacacattgt atcacaaggc ttcgtttcaa attaaaaaac gaagaaaaag
    48061 caaatgatga gatcttgaaa aatatggacg gtattgtaac ggtaatgcga gcgggagggc
    48121 agtatcaagt tgtcattggg aatcatgtgc cagtcgtgtt tgaagaggtt ataaaagcag
    48181 gaaaccttac ttttgatgag gctgtttcaa cagaaaaaat gagaccgttt gatatgctta
    48241 ttgatggaat atcgggctgt ttccagccat tccttgctat cctagctgct ggcgggatga
    48301 ttcgtggact gacagcattc cttgtcttct taggcgcgtt tgaccgcggt tctggcacat
    48361 ttgttatgtt tgataatatc ggtgattctg tattccaatt tatgcctgtt attatcggtt
    48421 taacagctgc ccgtaaattt aaagtaaatg aattcgtagg gatgttgctt ggcgcagcat
    48481 taatgaatcc tagcttgtcg ctggaagctt tgagtggtgc agcagaagca ccactaacga
    48541 ccatattttc tggcactatt tttgaagcac ctatttatca aacagtattc ggcattccct
    48601 ggattgcgcg taactacgct tcttctgtca ttcccattat ctttattgtg ttgttagcta
    48661 gccaaattca gaaaccaatt aaaaagttag tacctgagat gatcgcgaat ttcttcgttc
    48721 cattcttcac cgttcttatt acaatgcctt taggtttttt actcgttgga ccagtattca
    48781 cattcgcaac tgatatttta atggctggat ttgaaacact tttagcactt tcgccagtta
    48841 tttatggagc aatcgtaggc ttcttctggc aaattctggt gatgtttggt ctccattggg
    48901 caatcgtacc aatgggattg atgcaatttt ctgtgaatgg ttggcaaaat attatgacac
    48961 ctgttgcagt agtgtctttt ggacaaactg cagcacttac cgcactctac tttaagctta
    49021 gaaatccaaa agacaaagca attgcaatac cagccattgt ttctggtatt gtggggatta
    49081 cagagccagc tatttatggg tttacattac cacgtaaaaa aatatttatt tttacctgtg
    49141 tcggtggtgc gttagggggc gcgtatagtg ggttaatgaa tttaacttct tggaatcaag
    49201 gtggactagg catctttaca attcccaact atattagacc agatggtgat ttaacagatg
    49261 tgattaatgt attgattggg atcgccattg cgatggtggt atcctttacg ctgacttttt
    49321 tcttttggaa ggatgaagca ggtgaaacag atgatataca gaaaaaatca ggtaaagaaa
    49381 ttgtaaaaac accgatacaa ggacaaatcg ctccactaaa tgcagcaaag gatgcagctt
    49441 ttgcacaagg aacactaggt cgaggaatct tgatctaccc tgaaaaagga gaagtacgtg
    49501 ccccttttga cggaacagtc atgaccctgt tcccgacgaa acaggcaatt ggaatggttt
    49561 ctgaaacagg gctggaattg ctgattcatg tcggattgga tactgtgcag ttggaaggga
    49621 aatattttga aagcttagtg cagcaaggat aaagagtgaa acaaggcgat ctgttgctac
    49681 gcttcgacaa aaagaaaatc gaaaaggaag gatacagttt agaaacacct gtgatcgtca
    49741 ccaattacac ggattatttg gatattttgg aaaaccaagg ggagacggtc gggccagacg
    49801 atgcacttat caccgcactg acctgaacgg ttaggtgaag aatggactag gcttaactat
    49861 tctttttatt ccacttcttt attttcaaaa ttgaaaggct ctttcatgat ggctgagctg
    49921 atagggcgtt tatcgaattt ttgaccgaca gaaattagat ggactaaatt ctgttgataa
    49981 ttttctgcaa aaacacaact atataacacg tctagaagat aacaaattga tgtattgatc
    50041 gtaaaattac caatttttga atacagtcgc tcgcgtgtcg taagtcgcaa agcggctgta
    50101 ctcattgcag atagcgttga atcaccgata ctggtcaaag ctaaaattgg aacatgattt
    50161 tgtttcaata tttctgcggt acgaaggatc acattgtttt cacccgtata agaaatcaga
    50221 atcccgcaca tatcactcgt gcaattgtac gcttcataga atgattcacc aaaagtggtg
    50281 acaatcgtga catccatttt gatgcgcttc attttcagca tgaaatcttg agaaatcaaa
    50341 gtattcgagt tactggcgaa aatcacgatt ttttttgctt gaagcattag ttgtttggct
    50401 ttttgcagtt ggtcatgatg aatgagagaa agtgtatcat gaatcgtcat ctgttctaaa
    50461 gaagccattt tatttgcgat cgtcataata ccgtctgtgg tggaaaaagg aagattcgca
    50521 tctacgtctt gaaaattccc ttcaagatag gctgtttcag ctaaaaaagc attacgaaaa
    50581 ctgacccagc catcaaaacc catttttttt gctacacgga tgagtgtaga ggggtggaca
    50641 taacatcgct cagcaatcgc ttggatggtc atttcgccaa gtgctggaga atcgcttaag
    50701 agaaactgga tcacttcatt ttctgaagga gaaaaggaga tttctttgat tttttcacgt
    50761 aataacatgt agataccttc ttcttatttt tttacgtaaa ttataacttt tttgaaaatg
    50821 atttgtaaac ggatttatac tatataactt aaaatagaca ttttttgaca agatatttac
    50881 aaaaatccac tagaaaacag taaattttgt aaatgttact ggaaagaaac agatttctat
    50941 tgtttgtttt gtttacaaaa aagataatga aagtgtaaaa aagtaaagga gtgagaacat
    51001 ggcaaaagga atcaaaatcg ctacgattgg tggaggcagc agttacaccc ccgagttgat
    51061 ggaaggattt atcaaaagat atgaggaatt accgatacga gaaatctggc tcgtagatgt
    51121 agaagaagga aaagaaaagc ttgagatcgt tagtcagatg tctcagcgta tgtgggatgc
    51181 ttctccgtat gaagtcaaga tctatgcaac tctggatcgg gaagaagcat tgaaagatgc
    51241 ggattttgtt acgactcagt ttcgtgtcgg tcaattagat gcacgaatca aagacgagcg
    51301 gatccctagt tattatggga tgattgggca agaaacaaat ggtgctggcg ggatattcaa
    51361 agcttttcgt accgttccag tcgttttggc aatcgttgag gacatgaagc gtttatgtcc
    51421 aaatgcttgg ctgatcaatt tcgcgaaccc aagtgggatg gtgacagaag cagttatcag
    51481 atatggcaaa tgggataaag tgattggatt atgcaatgtg ccagtgatgg cgatgatgac
    51541 agaaccagca atgcttggca agaaaaaaga agagctgatc tacaagtttg ccggtctaaa
    51601 tcatttccat tggcacaaag tagcagataa taaaggaaat gaccgcacgg cagaattgat
    51661 tgatcgttta tacgatgaag agaatggatt accgaaaaat atctttgata tcccgttttt
    51721 caaagaacaa ttaaaacaaa tgaacatgat cccttgtggc taccaccgct attactatcg
    51781 tgaagaagaa atgctggaac atgctttaga agaatatcaa acgattggta caagagcgca
    51841 gcaagtgaaa gaaacagaag cagagctatt tgaattatac aaagatccag aattagacca
    51901 taaacctgaa caattgcaaa aacgaggtgg cgcttattat tcagacgctg cctgtgaaac
    51961 gattgcttca atctatggaa ataaagaaac acagatcgtg gtatctacaa agaatgatgg
    52021 agctgttcct gacctgccgg cagattgtgt agtagaagtc actgcctatg ttggcgcaca
    52081 aggagcgaga aatgtagcgt ttggttctct tcctgctgct gaaagaggct ggttgcaagt
    52141 aatgaagaat atggaattgc tgacgatcga agcagctgtg actggtgatt atgggacagc
    52201 tttacaggcg tttacgatca atccacttat acgatcagga aagacagcaa aacggatcat
    52261 ggatgagttg tttattgcac accagcagta tttaccgcaa ttcaaggaga caatcagccg
    52321 attaaaagaa gaaggaatcg aagttcaaga tgaactggca agagaattat caggtaatac
    52381 acatataatg gaggaagtat aatgggattc agaaaagatt ttttatgggg cggtgctaca
    52441 gcagccaatc aatgtgaggg cggttatgat gaaggtggac gcggactagc aaacgtagat
    52501 gttgtgccaa tcggaaaaga ccgttttcct gttatcaccg gaaagaaaaa aatgtttgaa
    52561 tttgatgagg atcattttta tccagcaaaa attgcaatcg atatgtatca tcgttataaa
    52621 gaagatattg ctttgtttgc agaaatgggg ttcaagactt atcgaatgtc catcgcctgg
    52681 agtcgtattt ttccaatggg ggatgagaaa gagccgaatg aagctggctt gaaattttat
    52741 gaggatgcct ttaaagaatg ccaaaagcac ggaattgagc cacttgtgac gattacgcat
    52801 tttgattgtc caatgcattt gatcaaagaa tatggtgcgt ggcgttccag agaactcgtt
    52861 ggtttttatg aaaatctgtg tcgagtcatc ttcaatcgct ataaaggact tgtcaaatat
    52921 tggctgacat tcaatgagat caatatgatc cttcatgcac cattcatggg cgcagggctg
    52981 tattttgaaa aaggagaaaa tgaagaacaa atcaaatatc aagcggcaca tcatgagttg
    53041 gttgcaagtg ccatcgcaac gaagattgct catgaagtag atccggagaa caaagtaggc
    53101 tgtatgctgg cagcaggagc atattatcca tacacttgca atcctgaaga tgtttgggct
    53161 tctagaaaat cagatcgaga aaattatttc ttcattgatg tccagtcacg aggagaatat
    53221 cccgcttatg cattgaaaga aatggagcga aatggcattc agatcgaaat ggaaccaggg
    53281 gatgccgcat tgctgaaaga acatactgtt gattttatct ctttttctta ttatgcttcc
    53341 cgagtttcga ctaccgacaa cgaactcttg gaacagactg caggaaatat ctttgcttct
    53401 gtcaaaaatc catatctgca agcaagtgaa tgggggtggc aaattgatcc attaggcttg
    53461 agaataacga tgaatgatat ttatgatcgt tatcaaaaac cattgtttat tgtagaaaac
    53521 ggcttaggtg cagtcgatac gccagatgaa aatggttatg tggaagatga ttaccgaatc
    53581 gattacctgg cagcgcatat ccaggcgatg aaggatgcag tagaactaga cggagtcgat
    53641 ctattaggct atacgacttg gggatgtatt gatttagtat cagctggtac cggtgagatg
    53701 aagaaaaggt acggattcat ttatgtcgat cgtgataacc aaggaaatgg cacattagcc
    53761 cgttcaaaga aaaaatcatt caactggtat aaaaaagtaa ttgattcaaa tggagaaaat
    53821 ctttcgaacg attgatcaat gatttataaa agtctattgt ttttcagcgg atttttcgag
    53881 tataataaag ctattcgaga aacgcaagca gaacaataga ctttttttcg cattggctgt
    53941 aaagtatgct tacttgatta agtaaaggag aatctacata tgtatgatgt aatcgttatt
    54001 ggcgcaggtc ctgctgggat gactgctgct ttatatgctt cacgttcaaa cttatccgta
    54061 ttgatgatcg aacaaggtgc ccctggcggc caaatgaaca atacggcaga aatcgaaaat
    54121 tatcctggat tcgattcgat catgggcccc gatctagctt ataagatgta tgaaagtgtc
    54181 tcaagatttg ggacagaaaa tgcttatgga atcgttcaag atatcaagga tcacggcagc
    54241 cataaagaag tcatctgtga agataaaagt tacgaaggaa aaacagtcat tatcgcaaca
    54301 ggatgtatcc accgtaaatt aggcgtacct ggcgaggaag aatttgctgg acgaggcgtt
    54361 tcttactgtg cagtctgtga tggtgcgttt ttccgtaaca aacgtctagt agtcatcggg
    54421 ggtggggact cagcagtaga agaagccatc tatttgacac aatttgcttc tgaagtagtg
    54481 atcgtccacc gtagagatga attacgtgca caaaagatca tccaagaccg tgcatttgca
    54541 aatgaaaaaa tttcctttat gtgggataca gtcgttgacg aaatcgttgg aaacgaaatg
    54601 gtagtgacag gtgtgaaagc acgtaatgtc aaaaccggca atgaatttga tttagcagca
    54661 gacggagtat tcatctatgt tggactggat ccattaactg caccttttag agaagcaggt
    54721 ttgacgaacg aagctggctg gattccaaca gacaacgaaa tgaaaacgaa tatgccaggc
    54781 gtttttgcca tcggagatgt tcgtgaaaaa gatttgcgac aaatcactac agctgttggt
    54841 gacggcggta ttgctggaca acaagtgtat aaatacttgg agtctttaga agaaacaaca
    54901 gcataagttt aaaaagagta gaagaacggg ttcaattgtt cttttactct tttttttcgc
    54961 gtgaaattta ctgaatctga tttatgcgaa cacgtccaac accttttgat aaaagaatcc
    55021 tttcttgatt taagcgtttt ttggtcctca tactttcata tctaataagt gaatgctata
    55081 cttattaaga accatatttc agaaagaggt gttattttca tgtcttggga acaagtgtat
    55141 gaacaatggg tacatgaaga aaatttagaa gaaaacttaa aaaaacaatt aaatgatctg
    55201 ggtgaagatc ctgaaaaact tgaagatgct ttttatgcac cgttggaatt cggtactgca
    55261 ggtatgcggg gaatcttagg acctgggatc aatcgaatga acatctatac ggtaagacaa
    55321 gcaacagaag gactagcacg tttcatggat acacaaggtg aagaaacaaa acgccgaggt
    55381 gtcgctatcg cttatgactc acgccatcag tcaccagaat ttgcaatgga agcagccaaa
    55441 acactagcac atcacaatat cccgtcttat gtattcgaaa gtctgcgtcc aacaccagag
    55501 ctttcttttg cagttcgtca tttgaagacg tttgcgggaa tcatggtaac cgcatctcac
    55561 aacccagcgg cttacaatgg atataaagtc tacggagaag atggcggaca aatgcctcct
    55621 gcagacgcag atgcattgac gaaatatgtc cgtgaagtca gcaatccgct aaaagtcgaa
    55681 gtattatctg acgaagaagc taaacatagc gggttgatca cgatcatcgg agaagaagtg
    55741 gacgctgctt atctagaaga aatcaaagca gtgacgattg atcgtgaatt ggttgaaaca
    55801 atggggaaag atttgaaact tgtgtacacg ccacttcatg gaacagggaa aatgttgggc
    55861 gaacgtgcgt tgaaacaagc tggattcgaa caattcgtac tagttcccga acaagctgtg
    55921 gcagatccag atttcactac ggtcaaatcg ccaaatccag aagaacattc agcttttgaa
    55981 tatgctatac gacttggaga aaaagaagga gctgacttgc tgattgcaac agatcctgat
    56041 gcagaccgtt taggcgcagc ggttcgtttg ccagatggtt cttatgaagt attgactggg
    56101 aaccaaatcg gagcattgtt tgctcaatat attttggaag cacatcaatc aagaggcact
    56161 ttaccagaaa acgcagcggt attgaaatca atcgtttcca gcgaattgcc tacagcaatt
    56221 gcggaacact acggtgctac catgttcaat gtgttgacag ggtttaaatt cattgctgaa
    56281 aaaatccaac aatttgaaga agaccattca catactttta tgtttggatt cgaagaaagt
    56341 tatggttatt tagttaaacc ttttgttcgc gataaagacg cgatccaagc attgctttta
    56401 ttagcagaag tagcagctta ctataaaaaa caaggcaaaa cgttgtatga cggcttgcaa
    56461 acgatttttg aaacctatgg ctatttcgca gaaaaaacga tttctgtgac aatgagcgga
    56521 caagaaggtt ctgcgaaaat ctctgctttg atggaaaaat tccgtaaaga agcaccgaca
    56581 gaatttgccg gagcaaaagt tattcaaaca gaagacttta aagaattgac gagaacagac
    56641 cgtgaaggaa acgtagaaaa actaacaaca ccgccatccg acgtattgaa gtatacattg
    56701 gaagaaaatg gctggattgc tatccgccct tcaggaactg agccgaaaat caagttttat
    56761 attggtgtaa aaggaagttc tacagaagac gcagccgaaa aagtagcctc ttatgaagcg
    56821 attatcaaaa agatgacaga agaataaaaa acttgagact gatggaaaaa gtggtaaact
    56881 ttagaagtca gtcttttttc aagttggagg tgaatggatt gccagacacg caaaaatcaa
    56941 aagtagagaa agtcagtcaa ttattcaaaa tgttgagtga tcccactcgt cttaaaattc
    57001 tgctatatct gaaagacggg gaacaaaatg tgacagcaat cacccaagca gtggaaatgg
    57061 agcagtctgc tgtatcgcat caactacgtt tattaagaga aaatcatgtc gtcaagtctc
    57121 atcgcgaggg aaaagcgatt ttgtatagtc ttgatgattc tcatgtactg gatattttga
    57181 accagacact caaacatgtg gaacatcaat aacgataggt gcaaatagat aaacactgga
    57241 gtgtgacaac gaagaaattt cgttgtcaca ctccagtgtt ttttaagaaa aagacaaaaa
    57301 tgatcaatcg cttttttctg ttgtttcagt tccataataa ctatgatctt ctaggtctaa
    57361 agcattcaaa ataagcgaag ccgtttcttc aatagataaa gatgctacat tgatcactac
    57421 acatcctagt ttttcataaa gatcattcgc aaaatcgagt tcagctctaa ttttttcgat
    57481 atcggaataa gcagtatcag ggtttaatcc gtaagagcgc atccgttctt tacggatatt
    57541 atttaaaata tctgggttat ttgttagtcc cacgattttt ttaggatcga tctcccaaag
    57601 ctgtttggga atatgtgctt gtgggatcaa aggtaaattg gcaactttta aatttttgtt
    57661 agctaaaaac aagctcaaag gcgttttgga agttcgggat acgcctagca aaacgacatc
    57721 tgcttctaaa aaaccacgag gatctttccc atcatcgtat tttacagcaa attccattgc
    57781 tttgatccgt ttgaaatagt tctcattcaa taaatggagg gcaccaggtt gccgaattgg
    57841 cttgatacca gttaaacgtt caatttctga tatcggtggc gtcatcacat caaaactaaa
    57901 taattgatgt tcttcacaaa aagaacgtgt tatctcgatc agttcttgat tgattaatgt
    57961 atgcaggaca agtgcttttt cttctaatgc atcttgcaat gcctgcctta atgttttttc
    58021 atctgtcacg aacgttctgc ggaataggtt gaactcaacg gtaggatact gagccatagt
    58081 agcttgcgct agttttgtcg cagtttctcc agcagagtcg gagatgacaa aaaaagtaac
    58141 gatttctgta gaatcggaag tcatggataa aacctcactt gcattttttt tatgtttatt
    58201 ataccatact ctagaatgcg tacttcgtaa tcattttgat ttggagggat aagtcatgaa
    58261 aaaacagaca ttgatcgaac aatcattaga aacattgaaa aataacggtt ttaaatatac
    58321 taaaaaaaga gaagcattgt taacttattt agtgaagcgt aaccgctacg tatctgctaa
    58381 agaagttttt gactatatga atcaggaatt caaaggggta agttatgata cgatttaccg
    58441 gaatctccat gattttgcag agatcgattt actggaagaa accgatttaa atggagaaat
    58501 gaagtttcgt tttcattgct gtcaggggaa aatcggccat caccatcatt ttatctgtac
    58561 agtgtgcgga acaacgaaag aaatccatat gtgtccgatg aattatttcg aagaacagct
    58621 aggtggatgc acgatcgaag ggcatagatt cgagattttc ggaagatgtg aaaattgtca
    58681 ataaggattt aaataaaata acatttgata attttaaaaa aaagcaagat ttccttgacg
    58741 gtcaaaacag cattagatat aatatttaca ggacagtctt gttttttgag atatatttca
    58801 ataacaatta cggttacttt ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc
    58861 gttcgcaaaa acgaatcact tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa
    58921 gcgggtactt tacaagaatc tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc
    58981 aagaaaaaat ctgaagcagc tagaaaacgc aaaaaattct agttctgtga tgaattggag
    59041 ttggtagtgt gtcactacta agtagattaa acgatgacat gaaagcagcg atgaaagcga
    59101 aagataagga atctttacaa gtcatccgaa tgatcaagtc atctatacaa aacgagcaga
    59161 ttaaagaagg tcacgacttg acagaagaag aagaactaac ggtgttgtct cgtgaaatga
    59221 aacagcgacg tgattctttg catgagtttg aagaagcagg acgtgacgat ttagctgaaa
    59281 aagtaaaaag tgaaattgta atagtcgaaa aatatatgcc ggaacaactt tccgacgaag
    59341 aaattcgaca acttgttcaa gaagcaatca ctcaaactgg tgcttcatca atgaaagaat
    59401 ttggcaaagt tatgggtgca attatgccta aagtcaaagg aaaagcagat ggaaaccaag
    59461 tgaatgcgat tgtgaaagaa ctattacaat cttaatttta tcatttaaac tgcggacagt
    59521 gattatccgc agttttttgt gttcgtaaaa aaagaacta
//
LOCUS       DACDCJ010000007        58445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDCJ010000007 DACDCJ010000000
VERSION     DACDCJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.007"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(69..197)
                     /locus_tag="IV038_000446"
     CDS             complement(69..197)
                     /locus_tag="IV038_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8340694.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(200..1573)
                     /gene="rlmD"
                     /locus_tag="IV038_000447"
     CDS             complement(200..1573)
                     /gene="rlmD"
                     /locus_tag="IV038_000447"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8340695.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1684..2739)
                     /locus_tag="IV038_000448"
     CDS             complement(1684..2739)
                     /locus_tag="IV038_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8340696.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2749..4179)
                     /gene="gatB"
                     /locus_tag="IV038_000449"
     CDS             complement(2749..4179)
                     /gene="gatB"
                     /locus_tag="IV038_000449"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP8340697.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4176..5648)
                     /gene="gatA"
                     /locus_tag="IV038_000450"
     CDS             complement(4176..5648)
                     /gene="gatA"
                     /locus_tag="IV038_000450"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP8340698.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5648..5953)
                     /gene="gatC"
                     /locus_tag="IV038_000451"
     CDS             complement(5648..5953)
                     /gene="gatC"
                     /locus_tag="IV038_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP8340699.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6059..8095)
                     /gene="ligA"
                     /locus_tag="IV038_000452"
     CDS             complement(6059..8095)
                     /gene="ligA"
                     /locus_tag="IV038_000452"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP8340700.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8112..10346)
                     /gene="pcrA"
                     /locus_tag="IV038_000453"
     CDS             complement(8112..10346)
                     /gene="pcrA"
                     /locus_tag="IV038_000453"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP8340701.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10501..12060
                     /locus_tag="IV038_000454"
     CDS             10501..12060
                     /locus_tag="IV038_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP8340702.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12171..14102)
                     /locus_tag="IV038_000455"
     CDS             complement(12171..14102)
                     /locus_tag="IV038_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8340703.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14120..15034)
                     /gene="pfkB"
                     /locus_tag="IV038_000456"
     CDS             complement(14120..15034)
                     /gene="pfkB"
                     /locus_tag="IV038_000456"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8340704.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15031..15783)
                     /locus_tag="IV038_000457"
     CDS             complement(15031..15783)
                     /locus_tag="IV038_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8340705.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15942..16151)
                     /locus_tag="IV038_000458"
     CDS             complement(15942..16151)
                     /locus_tag="IV038_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340706.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16162..17490)
                     /locus_tag="IV038_000459"
     CDS             complement(16162..17490)
                     /locus_tag="IV038_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340707.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17855..18442)
                     /locus_tag="IV038_000460"
     CDS             complement(17855..18442)
                     /locus_tag="IV038_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP8340708.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18525..19775)
                     /gene="clpX"
                     /locus_tag="IV038_000461"
     CDS             complement(18525..19775)
                     /gene="clpX"
                     /locus_tag="IV038_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP8340709.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20022..21311)
                     /locus_tag="IV038_000462"
     CDS             complement(20022..21311)
                     /locus_tag="IV038_000462"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP8340710.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21465..22397)
                     /locus_tag="IV038_000463"
     CDS             complement(21465..22397)
                     /locus_tag="IV038_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340711.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22554..23213)
                     /locus_tag="IV038_000464"
     CDS             complement(22554..23213)
                     /locus_tag="IV038_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP8340712.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23216..24241)
                     /gene="sppA"
                     /locus_tag="IV038_000465"
     CDS             complement(23216..24241)
                     /gene="sppA"
                     /locus_tag="IV038_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP8340713.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELALNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKFDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24254..24544)
                     /locus_tag="IV038_000466"
     CDS             complement(24254..24544)
                     /locus_tag="IV038_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340714.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24954..26759
                     /gene="glmS"
                     /locus_tag="IV038_000467"
     CDS             24954..26759
                     /gene="glmS"
                     /locus_tag="IV038_000467"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP8340715.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26831..28186)
                     /locus_tag="IV038_000468"
     CDS             complement(26831..28186)
                     /locus_tag="IV038_000468"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP8340716.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28210..29379)
                     /locus_tag="IV038_000469"
     CDS             complement(28210..29379)
                     /locus_tag="IV038_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340717.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29376..30263)
                     /locus_tag="IV038_000470"
     CDS             complement(29376..30263)
                     /locus_tag="IV038_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP8340718.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30452..34132)
                     /gene="nifJ"
                     /locus_tag="IV038_000471"
     CDS             complement(30452..34132)
                     /gene="nifJ"
                     /locus_tag="IV038_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP8340719.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34330..34851
                     /locus_tag="IV038_000472"
     CDS             34330..34851
                     /locus_tag="IV038_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8340720.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35550..36115)
                     /locus_tag="IV038_000473"
                     /pseudo
     CDS             complement(35550..36115)
                     /locus_tag="IV038_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36294..36950)
                     /locus_tag="IV038_000474"
     CDS             complement(36294..36950)
                     /locus_tag="IV038_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340721.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37518..38453)
                     /gene="whiA"
                     /locus_tag="IV038_000475"
     CDS             complement(37518..38453)
                     /gene="whiA"
                     /locus_tag="IV038_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP8340722.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38487..39485)
                     /locus_tag="IV038_000476"
     CDS             complement(38487..39485)
                     /locus_tag="IV038_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP8340723.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39482..40366)
                     /gene="rapZ"
                     /locus_tag="IV038_000477"
     CDS             complement(39482..40366)
                     /gene="rapZ"
                     /locus_tag="IV038_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP8340724.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40501..41232)
                     /locus_tag="IV038_000478"
     CDS             complement(40501..41232)
                     /locus_tag="IV038_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP8340725.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41236..42501)
                     /locus_tag="IV038_000479"
     CDS             complement(41236..42501)
                     /locus_tag="IV038_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP8340726.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42764..45580)
                     /gene="uvrA"
                     /locus_tag="IV038_000480"
     CDS             complement(42764..45580)
                     /gene="uvrA"
                     /locus_tag="IV038_000480"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP8340727.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45590..47584)
                     /gene="uvrB"
                     /locus_tag="IV038_000481"
     CDS             complement(45590..47584)
                     /gene="uvrB"
                     /locus_tag="IV038_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP8340728.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47843..49345
                     /locus_tag="IV038_000482"
     CDS             47843..49345
                     /locus_tag="IV038_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8340729.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49347..50084
                     /locus_tag="IV038_000483"
     CDS             49347..50084
                     /locus_tag="IV038_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8340730.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50452..51618)
                     /gene="dnaJ"
                     /locus_tag="IV038_000484"
     CDS             complement(50452..51618)
                     /gene="dnaJ"
                     /locus_tag="IV038_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP8340731.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51769..53598)
                     /gene="dnaK"
                     /locus_tag="IV038_000485"
     CDS             complement(51769..53598)
                     /gene="dnaK"
                     /locus_tag="IV038_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP8340732.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53649..54212)
                     /gene="grpE"
                     /locus_tag="IV038_000486"
     CDS             complement(53649..54212)
                     /gene="grpE"
                     /locus_tag="IV038_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP8340733.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54306..55349)
                     /gene="hrcA"
                     /locus_tag="IV038_000487"
     CDS             complement(54306..55349)
                     /gene="hrcA"
                     /locus_tag="IV038_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP8340734.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55584..56750)
                     /locus_tag="IV038_000488"
     CDS             complement(55584..56750)
                     /locus_tag="IV038_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP8340735.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56766..56993)
                     /locus_tag="IV038_000489"
     CDS             complement(56766..56993)
                     /locus_tag="IV038_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP8340736.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57380..57636)
                     /locus_tag="IV038_000490"
                     /pseudo
     CDS             complement(57380..57636)
                     /locus_tag="IV038_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57979..58425)
                     /locus_tag="IV038_000491"
     CDS             complement(57979..58425)
                     /locus_tag="IV038_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8340737.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
BASE COUNT        16086 a        12690 c        10210 g        19459 t
ORIGIN      
        1 aaagagccac taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt
       61 tagcacgtct atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta
      121 ggtgtttcca tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt
      181 ttttcttttt ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat
      241 gggtttgcgg gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa
      301 ataacttagc atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg
      361 gtgacatttc gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa
      421 ctacgatcac agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt
      481 ttccgacttc ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt
      541 caattgcttc tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca
      601 agccaatcgt acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg
      661 caaactcgat ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg
      721 actttgctga gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat
      781 ataaaacttt ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga
      841 tgaccgaaac cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa
      901 agaatttttc tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac
      961 ggataatgat gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga
     1021 accgttgcaa gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat
     1081 aaaaatcttc gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact
     1141 gtccatcgat acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca
     1201 ttccgaccgt tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct
     1261 ctacttgttt ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga
     1321 tacctatgcg aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa
     1381 atgtttcgac ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt
     1441 cttctccagg gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca
     1501 ttccttcata actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt
     1561 gctgttctgt catattttgc tcctttaagc ctatttttca atcctactga actctactat
     1621 aaatcagcta attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt
     1681 tgattaattc ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc
     1741 atccgttaaa cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc
     1801 atctgtacct gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg
     1861 ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat
     1921 cattactggt cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata
     1981 gattattctt ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa
     2041 gatctcaaag atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt
     2101 ggcatctggc gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat
     2161 cgaagcattt ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac
     2221 tcgagggagc atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag
     2281 ttctgatgga acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa
     2341 gtaactgtta tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc
     2401 tgctgctgct ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt
     2461 accagctggg atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac
     2521 ttcgttgatt gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac
     2581 tctacatgct tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc
     2641 aaatcctgct tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt
     2701 tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc
     2761 taattcatct ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt
     2821 catgatttga ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac
     2881 agattgttgg ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga
     2941 taattgcacc caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa
     3001 ttcacggaag acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat
     3061 cattcctgct aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt
     3121 caagtaagca gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt
     3181 ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc
     3241 atactctgga agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg
     3301 caaactagct tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg
     3361 ctctgggaag taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt
     3421 cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt
     3481 tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt
     3541 tttcaattct gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc
     3601 atcacagcgc atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat
     3661 tgatcgcaat gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga
     3721 aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat
     3781 accatgcatg ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt
     3841 ctttttacct tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg
     3901 ggaaatttga tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt
     3961 atctttagag atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc
     4021 tttattcatt actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt
     4081 tggttctgca ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc
     4141 gacgtggact tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata
     4201 gctggttgtt gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc
     4261 gttttttcat caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa
     4321 cctgccggta cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca
     4381 ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt
     4441 gaagatggcc cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc
     4501 aatgtacgga cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag
     4561 aatgtaccta acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg
     4621 acatatacat catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg
     4681 aatcgttgca agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg
     4741 tattttgaat gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct
     4801 gctgctttca atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta
     4861 ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact
     4921 gaaacgcctg aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat
     4981 gcattatctt tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca
     5041 atcaaaccaa aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa
     5101 gctgctggtt ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca
     5161 gctgcaactg ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa
     5221 gcattttttg ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc
     5281 atgtttagct taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca
     5341 tcataaattg gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt
     5401 gttacgatat tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc
     5461 cctttttcat ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat
     5521 gcttcgacct caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt
     5581 acagtgattt ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat
     5641 tctgtcatta tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt
     5701 cttctgtttc cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat
     5761 cttgtcgcat cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat
     5821 ctacttcttc cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg
     5881 caagttcttc atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt
     5941 cactaatcgc catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta
     6001 acacaagagt gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc
     6061 attcttctac tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt
     6121 cgattcctaa ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga
     6181 tatccgtttt tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt
     6241 ttgcttcttc acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa
     6301 aaggagactc gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc
     6361 ctgcttgttt taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta
     6421 cactgtcggc aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt
     6481 tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga
     6541 catgtcggat tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga
     6601 ttgctgtcaa aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt
     6661 cttctgtcaa tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt
     6721 ctagtacgcg cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca
     6781 aaccttcttt catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat
     6841 ccaaatggac aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc
     6901 tgtctttagg tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt
     6961 tataaatcag aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat
     7021 gcaggctggc acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg
     7081 gcgttactac ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt
     7141 cttctggcgg aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt
     7201 gatcttgtag cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat
     7261 ccacacgttt gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata
     7321 gacgtttttc ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact
     7381 gggtttttgc ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc
     7441 ttttcgctgt gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat
     7501 tcgcaaagat atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt
     7561 ttggcatgaa acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga
     7621 ccgaccggac agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag
     7681 tcgcacctct cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt
     7741 ttagttcaca gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag
     7801 ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga
     7861 ctttttcaaa accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga
     7921 tcaaatcagg atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat
     7981 agtcttccac tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg
     8041 tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt
     8101 accctccact actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct
     8161 tgacttggga aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact
     8221 gttcctagac cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc
     8281 cctccagtag cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct
     8341 gtttttttag cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt
     8401 aactcctgat cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat
     8461 aatgttctgg agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca
     8521 tatgcgagac ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga
     8581 aaaaccccct cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca
     8641 tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca
     8701 agtgccagat catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca
     8761 ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt
     8821 tcgatgcgtg cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta
     8881 cgatccaaaa cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact
     8941 tcttggatca tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc
     9001 agatccacat tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt
     9061 agtttttcga tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa
     9121 ctgattgaat cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct
     9181 ttacgatcgt agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga
     9241 gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa
     9301 ttccgatgat tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct
     9361 cgttcattat ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag
     9421 ttttttggtt ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt
     9481 ttcgtggagc gatagttttg ttctagcaaa attactgcag catctggata atctttttca
     9541 aaatccaaga tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct
     9601 cccacgacgc acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg
     9661 tgatttgtat cttggtactc atctacatga atataatgga atttgttttg ataatatgtg
     9721 agtgaatcag gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc
     9781 atacattgat tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct
     9841 tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct
     9901 tgagagattg ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct
     9961 tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg
    10021 ttatacccga taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc
    10081 gatacccaga catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct
    10141 tttgcagcct tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc
    10201 tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc
    10261 attagcagta atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg
    10321 tttaataatt cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat
    10381 tatagcattt tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat
    10441 ggatttttcc ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga
    10501 atggaaaaaa aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa
    10561 ggaatatttg ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa
    10621 aagctaagcg atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta
    10681 attggcctag ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat
    10741 gaaccggata tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta
    10801 tcaatgaact ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt
    10861 gcgggccttt ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa
    10921 ggagctggac aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt
    10981 gcaagtagcg gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta
    11041 gaagaaatcc atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc
    11101 actgctaact tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac
    11161 cttccttctt tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc
    11221 atcttaggtt ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg
    11281 cctttaccag cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat
    11341 ttatggccta gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc
    11401 ttgccaatca ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct
    11461 tacggtggtg gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc
    11521 ggtagctttt atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat
    11581 tttgtcgtta ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca
    11641 attattttag tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct
    11701 gctcttgttg cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta
    11761 ttggaacgtc tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt
    11821 gattttccag taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg
    11881 cctaaaaata tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt
    11941 gatacgatca tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca
    12001 agaatcaagc aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa
    12061 cgaacatgga aaaactattt tacaacaact attcatctac actacttgat tctatatgat
    12121 tctataaaaa aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct
    12181 gctgctgcag gagcttttct caataaacca taaatcacac cagatacgac tgctccgata
    12241 gcaataaaca ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca
    12301 tgaggagcca gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca
    12361 ataataaaac ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc
    12421 gtgacaaacg atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg
    12481 aatttattct taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca
    12541 gccataacag aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct
    12601 gtaccaaaca cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca
    12661 gctaataatg caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc
    12721 aatgcatcat tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc
    12781 agtaagccaa acactggata aaagaggatc gttttaattc catccaatga ttttggtaag
    12841 ttcttgaata atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt
    12901 gcacctaaga atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc
    12961 aaaccagggc ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa
    13021 ccaaatgcag cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga
    13081 tttgataatt gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata
    13141 gcgattcctc caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg
    13201 taaattcttt gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca
    13261 tcaccatgga atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta
    13321 cggataccat cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct
    13381 acttttttgt ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat
    13441 cggtttttga ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc
    13501 gcttttttct tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct
    13561 gtaacggcaa caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct
    13621 tgttctgctt tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact
    13681 tcttctggtg tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga
    13741 gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg
    13801 aaaaacagat aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt
    13861 gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca
    13921 tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg
    13981 atatcggaaa tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt
    14041 ttatcagtcg cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc
    14101 atgattatcc tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat
    14161 caatcaattc gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc
    14221 cccatttaaa ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa
    14281 tcattgaatc ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat
    14341 aagttccttg ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct
    14401 gtgccccttc agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg
    14461 ttgtattgta aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa
    14521 caagtgcatt ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc
    14581 gaatgatatc aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg
    14641 acaatacgac gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt
    14701 cttcatctga aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt
    14761 tgatattgat tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt
    14821 caagccactt cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt
    14881 gagaatcgat ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga
    14941 agtcttcctt cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat
    15001 caattgatgg attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt
    15061 ttcacggaat tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc
    15121 caaaggtgca acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa
    15181 tgtctcttcg gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt
    15241 cgtgaatcct tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa
    15301 attttttagt tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt
    15361 tccacctaaa ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga
    15421 atttgtcact actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga
    15481 gccagcgtct aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat
    15541 ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa
    15601 taattgctct ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc
    15661 ttgtaaatca cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga
    15721 tttcacgatt ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag
    15781 cattttcacg atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa
    15841 tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa
    15901 aaagactggt tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg
    15961 cttcttgttt ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa
    16021 taaagtccca tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta
    16081 ggaacaaaaa agagacagac ggataataat gatgccggaa ccattattcg ataatagagt
    16141 tgtttatcca ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg
    16201 gtaatctaaa aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact
    16261 tcgccgcttt ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt
    16321 taacggtaat tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc
    16381 ttgatatttt tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc
    16441 ttcgattcca ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga
    16501 gcgattctcg tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct
    16561 ttctttataa tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt
    16621 tttaggaaca ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt
    16681 gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg
    16741 atcaaaccca agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca
    16801 gttaaagggg tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt
    16861 ttctacttgt tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact
    16921 ataatacatt tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg
    16981 aaattggttg caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc
    17041 gatttcatcc tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat
    17101 aaactcgcta taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat
    17161 gaagtctcga atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat
    17221 caataattga ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa
    17281 ccaactttcg tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata
    17341 cggatccgct tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa
    17401 aatagcccaa tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat
    17461 agtcatcaat aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat
    17521 ttaattataa ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac
    17581 gattacatga tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt
    17641 gaacgtacag ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca
    17701 gttgcaccaa aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa
    17761 aaagacaaga aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg
    17821 aaagagtttg actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg
    17881 ctgcccacgc ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat
    17941 tgcgatcaaa atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag
    18001 ggatcttatc tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt
    18061 gatacatctg gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg
    18121 cacgtagctg gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct
    18181 cagttttgga tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct
    18241 cgatcaaata aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat
    18301 tctttcgatc aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca
    18361 gagctatttc tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa
    18421 tttcagcttg atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg
    18481 gctgtcgcaa aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc
    18541 tctgaatctt tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata
    18601 atcacttttt cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct
    18661 tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg
    18721 gctctcaacg cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga
    18781 tattgtttga ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc
    18841 aatttctcta aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat
    18901 tttagtaaat cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg
    18961 ttttgtgtac caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg
    19021 ccatcaaatg caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct
    19081 tgatgtggat gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt
    19141 ttcagcaagg cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg
    19201 cttttacgcg caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc
    19261 acattgtaat cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca
    19321 taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt
    19381 gctaatgttt gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg
    19441 cttttttgca gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag
    19501 tgattgtata cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta
    19561 ttcaatactt caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct
    19621 tcataaaatt cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag
    19681 acccctggac cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag
    19741 cgtacggtct cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa
    19801 agtatgcaag aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa
    19861 aaagaaaaag tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa
    19921 caaaaaataa ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt
    19981 tctagaagta gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc
    20041 gctgtacctg tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct
    20101 tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg
    20161 atttctgcat tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg
    20221 atcacaagat ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct
    20281 tcttctgtag aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg
    20341 ttgttcaaga attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat
    20401 tcaacgattt ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc
    20461 gctgcttttt cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat
    20521 gttgatactt cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt
    20581 gcttttactt catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct
    20641 tgataatctt ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact
    20701 aattgatctt caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg
    20761 ccttttccgc catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta
    20821 tcgccatttt cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca
    20881 cgtttgatac gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact
    20941 agatctttat attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc
    21001 cagtcttgac ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg
    21061 ccagcttctt tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca
    21121 tataaagctt cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg
    21181 aatccaggta cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg
    21241 atttcgtctt gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat
    21301 gttgcagtca tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc
    21361 atgcatacct ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga
    21421 atccttgttt ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc
    21481 atttgctgca aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct
    21541 tcatacatcg tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct
    21601 tcgatgtatg tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt
    21661 aacacggaac taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc
    21721 tggtaacttg cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt
    21781 tcttcaatcg tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt
    21841 aattttgccc ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg
    21901 ataaactcat gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt
    21961 tccaacgaag gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt
    22021 tgtccggata attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca
    22081 taaaaatctg tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac
    22141 aagtcaaaat aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt
    22201 gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta
    22261 ttagcctcaa agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga
    22321 tattgctggt ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga
    22381 aaatcaatcg gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt
    22441 agcaaaacaa aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt
    22501 ttagatctga cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg
    22561 ttgacttcct ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa
    22621 aaatcgccta gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc
    22681 aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta
    22741 agttcttctt catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga
    22801 ccatgattga atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat
    22861 ccgtagatac tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca
    22921 agtgttaaac gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg
    22981 ataaaaaaac ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt
    23041 gtgtccgtat actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc
    23101 tctctttcaa aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc
    23161 tgctgttgct tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact
    23221 cacctccgta ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg
    23281 ataagatctg actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta
    23341 accatgtact agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa
    23401 gctgttgttc ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta
    23461 aacctacttc ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt
    23521 cttcttcaga tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt
    23581 cgatatatgc ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca
    23641 tatctttcaa cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc
    23701 ctgaataatt caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg
    23761 caaagatttt atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca
    23821 tgctgacata cattgggata tcatgttctt tacgaatcgt atcaaatttc ttagcgattt
    23881 ccgcactttc gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg
    23941 ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag
    24001 aagcaaataa acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga
    24061 ttttttgcgt actgtctccc tcttccaaca catttaaagc tagttcttca tccccatata
    24121 accaactgtt caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa
    24181 ctaacgaaac cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca
    24241 ttctttctcc tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag
    24301 tgtcgaaccg ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt
    24361 catcttctac cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt
    24421 ggatgctatc ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg
    24481 cttcgaattt ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat
    24541 ccatttttac tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg
    24601 atagataaaa acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata
    24661 taccaattta ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt
    24721 taaatgtttc cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca
    24781 ggtctgtgta aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag
    24841 ggactgcact ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt
    24901 cctgcagctg gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg
    24961 gaattgttgg aatgattgga ttaaaaaatg taacaccagg attgattgac ggcctagaaa
    25021 aattagaata ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt
    25081 atctggtcaa agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta
    25141 caggaaatat aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa
    25201 acgcccatcc acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg
    25261 aaaactttga agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg
    25321 atactgagat tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg
    25381 cgtttttaaa agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga
    25441 cagcacctga tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg
    25501 atggattcaa tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg
    25561 ttgagattga agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt
    25621 ttgcaggtga catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg
    25681 agaaaggtac ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc
    25741 gtcgtatcgc tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg
    25801 tcgatacatt gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg
    25861 cggggttagc aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt
    25921 tagccagtga atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct
    25981 tgagtcaaag tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg
    26041 gctacccttc acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct
    26101 ttacactgct attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg
    26161 cacaaatagc tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa
    26221 cttctcttgt attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg
    26281 tcatcgccca aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa
    26341 atgcctttta cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat
    26401 tgaaagaaat cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa
    26461 cgattgcttt gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg
    26521 gtgcgcatac tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta
    26581 ttgtctcaga gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt
    26641 atctaactac tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat
    26701 tacggggatt cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat
    26761 aaaagaacaa ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg
    26821 aaaatatctc ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat
    26881 agtaattaac tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac
    26941 cggaaggacg aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca
    27001 cttttttgat tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta
    27061 atttttgcgg atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt
    27121 tcatgatatt cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta
    27181 agaaaatcat gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt
    27241 ctacaacgta acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg
    27301 ctttgtggaa tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat
    27361 ttttttctgc caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc
    27421 caagttcatc caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg
    27481 ctcctttttc aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat
    27541 tgatatttaa cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa
    27601 acaaacgatt aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac
    27661 cggaaaggtc accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg
    27721 gaaattcttc tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag
    27781 catctaataa tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac
    27841 cgaaaaattt gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg
    27901 cgcttgcttt ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct
    27961 ggaacacttc gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac
    28021 ctgagattct tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct
    28081 ggcttaggac ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct
    28141 tgttggcttc accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac
    28201 tcctttaact taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc
    28261 tgatgtttct tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat
    28321 ggttgttgtc gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt
    28381 attttcttgt gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg
    28441 tgtaatctcc acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc
    28501 aattttacga gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc
    28561 accgatcgat gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact
    28621 gaaatcatag ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac
    28681 cgacttgctt ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat
    28741 acttaatact tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc
    28801 atctgtgacc atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt
    28861 gtcacctgta gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc
    28921 agatggtgtg aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt
    28981 ctcaaccgtt actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt
    29041 ttgaattttt agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac
    29101 gcggaacatc cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga
    29161 aagtttgaca gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga
    29221 aatcttgatt ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt
    29281 tggaacggac tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa
    29341 actgtaaaga atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc
    29401 ataccgtcta ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc
    29461 agttctctac gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta
    29521 tctaatgtaa tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg
    29581 acttcgctta taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata
    29641 ctttctgata gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact
    29701 gcaccgtcat gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata
    29761 tctgcatcta atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata
    29821 gtaattagcg caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag
    29881 atcatcttct catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca
    29941 agatgttcca gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg
    30001 tacgtaatga cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg
    30061 attcggataa caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc
    30121 agcagttgga tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc
    30181 aaaaaattca gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa
    30241 agttccccga tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag
    30301 tatatcatat gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca
    30361 taattttcag gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa
    30421 cttcagagaa ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt
    30481 gttccatttt tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg
    30541 tcgttttttc ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt
    30601 tcttgatttt ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt
    30661 cttggatagt cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg
    30721 aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt
    30781 ccaaagtcat tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc
    30841 aatagccaga agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca
    30901 aaccatgtgt gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg
    30961 cttcttcaaa agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat
    31021 aaacatttcc atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac
    31081 tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat
    31141 tggaatagac ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat
    31201 gatcgatccc gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc
    31261 tgttttttac gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc
    31321 gttcgctttc gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa
    31381 acatgtgtac caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata
    31441 cttctttgat tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat
    31501 aaccaaattc agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt
    31561 cattggtcgt ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg
    31621 cattcgcgat catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt
    31681 cgccacagcc tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca
    31741 agacagtatt ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc
    31801 aattcacagc ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg
    31861 cgggacaagc ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac
    31921 ggtattttac gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt
    31981 ccatttcctc ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac
    32041 attcattaca catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc
    32101 gcttttccat agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa
    32161 gtgtaccaac agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta
    32221 tataaccagt gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg
    32281 ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta
    32341 cgatagccat tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca
    32401 cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga
    32461 tccggcgtcc caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa
    32521 tgttattttc agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt
    32581 cgctccaaag agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat
    32641 agctatgcag gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg
    32701 ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt
    32761 tcgaatcata ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga
    32821 tagctgattt atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga
    32881 atgtttgcgg ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat
    32941 catccacgat ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat
    33001 caaaaactgc tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac
    33061 caataacagc cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag
    33121 gttctccacc tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat
    33181 gaggcaactt ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa
    33241 aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa
    33301 ctgatcccat agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt
    33361 cataagaagt tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta
    33421 atttatcata ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt
    33481 tcgtaccact aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt
    33541 tctcctgatt gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct
    33601 cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag
    33661 caagatgggc aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag
    33721 caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat
    33781 ttgtcgtcac tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct
    33841 tgtacatatt cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg
    33901 caccagtttt caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta
    33961 ctacttttac tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta
    34021 attcagccat ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat
    34081 aagaaatata tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc
    34141 atccttgttc atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt
    34201 tcttttattt ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga
    34261 caaacagtct aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg
    34321 tgttttaaca tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc
    34381 ctttatggtt catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg
    34441 tattgccgac aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg
    34501 caattgcagc gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac
    34561 gtgatgcacc ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa
    34621 atattcgata ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca
    34681 gtagataaac taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac
    34741 gaagctgagc aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt
    34801 gatgaatcta tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag
    34861 gagcgtagga caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg
    34921 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta
    34981 ttccgaataa atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa
    35041 gctgagcaaa ttatctccac caaataattt attcatattt ttgacttgcg catactaact
    35101 aattcttaaa ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg
    35161 gtgattcaca ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa
    35221 aaatagttgc ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg
    35281 tttattttaa acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg
    35341 gcaaacacaa atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact
    35401 cctaactcct ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca
    35461 cagcctagta tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact
    35521 ggctgatgaa atggattaat agatttcgat tattcaaagc gattttctat tggtggattg
    35581 tgttcccaac taacaagacg gattccaaag tcattatata actcttcaag agttaaccct
    35641 ttcctgttcc caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg
    35701 ccaagacctg gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa
    35761 atccagctcc tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata
    35821 attgttgtgc cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc
    35881 tacctgcatc tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc
    35941 tttccccatc tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc
    36001 ttggtggctt aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca
    36061 cagctataac aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc
    36121 gcaaatcacc tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc
    36181 tactgatcgt aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca
    36241 tcagctttta cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca
    36301 ccaaaattgt taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga
    36361 tttaacaatt cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat
    36421 tctttgtcat gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca
    36481 ctaggaaaag tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct
    36541 ggaaaataaa atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg
    36601 ctggctaaac gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca
    36661 tcaaattcaa ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca
    36721 agtctaaaag gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa
    36781 ataaatacaa taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct
    36841 gctcgaagta aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa
    36901 ataaaaagta ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc
    36961 cctttataaa catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat
    37021 aacgtattcc aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa
    37081 atcactccat ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc
    37141 taatccaatg aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt
    37201 aagaagagca gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc
    37261 cagttataga taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt
    37321 atgtacaaaa ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa
    37381 aacaatagaa gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg
    37441 aagaaaagca agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta
    37501 tcagtttttt ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt
    37561 ttgcgaattc ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct
    37621 aattctttca agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt
    37681 tcaggaaggg catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta
    37741 gaagccgcat caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc
    37801 gaatttctca tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca
    37861 ccaatcaatg ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca
    37921 ttgcgccgtt caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct
    37981 tggttatgct gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta
    38041 ttgactgaac ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt
    38101 cccataatct cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc
    38161 atcaacacat cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat
    38221 ttcatttttt tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa
    38281 tagatacgtc tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc
    38341 acaataccta aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct
    38401 cgatgaactt ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc
    38461 tcctcctttc tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat
    38521 tcttctacta ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg
    38581 gtggaaatca ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact
    38641 agatactcat catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc
    38701 aatactgtat cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca
    38761 tctgtgaaat gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca
    38821 gcagttgtct tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg
    38881 gtgaataaac ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact
    38941 tttcgagccg ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg
    39001 gttttacggt cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca
    39061 gcatgcaagg tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt
    39121 tttgctaata attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg
    39181 atcagattac cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg
    39241 aaaatatctt catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca
    39301 ccaggaggag ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca
    39361 acagtcacta ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc
    39421 agaccagtgc caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt
    39481 ttcacgaccg gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt
    39541 tatagttttt ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac
    39601 cgcctgtaca gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca
    39661 atacagtttc cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca
    39721 tcacataatc atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat
    39781 gagggttggg caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt
    39841 taaaaccaaa tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact
    39901 cattgatttt ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag
    39961 cttgggcttt caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac
    40021 cgtccatcgc cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt
    40081 ctgaagcatc taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca
    40141 gcatgttttg gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt
    40201 ttgtgacttt gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca
    40261 tattatcgat acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg
    40321 caccgctcat acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct
    40381 ctcacattgt gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt
    40441 cttttacaaa aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt
    40501 ttacttttcc gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt
    40561 ccgatctgct aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc
    40621 atgcatcaat gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac
    40681 cgcttcttct aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc
    40741 atgatagatc agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa
    40801 tccaagattt ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag
    40861 aatcgctact ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat
    40921 atgaaggata ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt
    40981 acaagtcaac acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag
    41041 aaacggcatt ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt
    41101 tgcatgattg aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg
    41161 gttgatcaag cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa
    41221 aaaattctcc atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata
    41281 gttatggaac atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa
    41341 cacagtagta ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt
    41401 ttcccgagca tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc
    41461 tgattgattc gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt
    41521 gacgaaacga gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct
    41581 caaattgatt tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt
    41641 ggcaaagcct gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc
    41701 gttataatct ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa
    41761 cagtggatga ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag
    41821 cacacgcata ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata
    41881 accggcttcg tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa
    41941 agcttttttt cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa
    42001 agcaactggg aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc
    42061 tctgacgatc aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct
    42121 tactttattg atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc
    42181 tgacagctct tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa
    42241 aagatatttt ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat
    42301 aaacacaggg tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg
    42361 ggtaggggct aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa
    42421 agaacgtgcc ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc
    42481 attttcaata ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc
    42541 aactacacaa ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt
    42601 gctcaattct tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg
    42661 tagaaagaac taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt
    42721 cattttttta tgttttttta actatctcag ctatctatcc tctttatacc attatttttt
    42781 tcaaatagtg acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg
    42841 caaggatcgt tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg
    42901 ttttgattac atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc
    42961 tttgtaatac atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat
    43021 ctagtatata gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc
    43081 gttgtgcctc accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc
    43141 caacatcaac aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta
    43201 cagcatcttc tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt
    43261 ctaatgtttc agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag
    43321 gcaaaaagtg catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc
    43381 cttttacgtt gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt
    43441 gagcaaataa cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg
    43501 gcgtccggcc gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg
    43561 aaattgtctt gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt
    43621 tctttaagat actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta
    43681 caaattctcc taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag
    43741 tgatcttgat tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt
    43801 tccctgaaag atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc
    43861 ctgcagccac gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat
    43921 ctgctgccca catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac
    43981 gcattttctt taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg
    44041 gttcatctaa aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga
    44101 ttcgctgcgc ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta
    44161 gtcccacatt ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa
    44221 ttactttctc ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag
    44281 acaactcatt tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat
    44341 tcaatcgata acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct
    44401 cacgcgtgaa atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt
    44461 cgaaagtcgt ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa
    44521 gatcatctgt cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg
    44581 tatctaaatc gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag
    44641 agctgattgg attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa
    44701 tcactaaatc ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc
    44761 caaaaggcgc attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat
    44821 aaggacaggc atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag
    44881 catagccgtc tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc
    44941 cttctttcac aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca
    45001 gctcaggcac ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct
    45061 ctttttggat tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg
    45121 ccaacacttg gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta
    45181 ctgattgact ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa
    45241 acaacaagcg taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt
    45301 tacttgttgt tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt
    45361 ccggcttatc catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc
    45421 tccgctgccc ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca
    45481 aacctgtaac gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat
    45541 gggcacgtgc accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag
    45601 cttttaattc taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg
    45661 ccgcattttt catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca
    45721 tttcttcata cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag
    45781 aaatcaaatc tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt
    45841 acttctgctg aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg
    45901 ttactttgtc tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg
    45961 tttggactaa ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa
    46021 caagggaaac ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat
    46081 caaattcacc taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac
    46141 tatgcaaata tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca
    46201 tttttttcgt caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt
    46261 cgcctactag atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca
    46321 gccctgtcgg acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc
    46381 ctggcgtcgc agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa
    46441 gtggccgatt gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc
    46501 tcgctcggtc accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg
    46561 ccacgatcaa aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt
    46621 tacgtccgtc catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca
    46681 tcatttcaat atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt
    46741 catttcgcaa gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct
    46801 ccatatgttc atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt
    46861 ctcctaaaac ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa
    46921 aaaactccac acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac
    46981 cgcgcactcg aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga
    47041 ctaagtcacg aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt
    47101 gtttttggta ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga
    47161 tcacatcatt tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat
    47221 catttacact tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct
    47281 gataataatc ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga
    47341 attccccgta cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat
    47401 tcactttctc aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata
    47461 agatttgtgc ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct
    47521 gatcaccagc cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa
    47581 tcattcttcg tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag
    47641 gaaagcaaag gcattcctta ctaaaaaatt ataccaaacc gaacatactt tcgccacttt
    47701 tttatcttat tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt
    47761 cattttttcg agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata
    47821 ttggaaattt aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt
    47881 aatgacagga cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac
    47941 ggcagagtca agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt
    48001 aattgccagt gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga
    48061 aggcatcgat gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat
    48121 ggactaccgt ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat
    48181 gattgccgga atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta
    48241 ctttgatagc gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga
    48301 agatttgaaa ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag
    48361 cgaccatgaa tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta
    48421 cgacgggtta aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta
    48481 tgcgatcaaa caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata
    48541 tgcatttgcc gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt
    48601 gaaagaatta aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg
    48661 ttcagaaact tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca
    48721 aaacaactat caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc
    48781 ctttgctttg gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg
    48841 cgctcttcgc acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt
    48901 tcttgcgttc tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt
    48961 ccttgctggt gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg
    49021 aggcgggatc aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc
    49081 ttataatcgg acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc
    49141 attcgtcaat cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt
    49201 agtcgaattg ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt
    49261 ttacttgatc attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa
    49321 agttttagaa aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca
    49381 aaaaatacgg cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg
    49441 tcgtctgtat cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc
    49501 gtttagaaga accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa
    49561 gcacagatat caacttggtg agacaacata tcggaatggt attccagcac tttaacctat
    49621 tccctcatct ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat
    49681 ctagagaaga agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata
    49741 aaaaagatat gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc
    49801 gtgctctagc gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc
    49861 ccgaaatggt cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga
    49921 tggttatcgt tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca
    49981 ttgacgacgg aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa
    50041 atgaacggac aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc
    50101 ccggtcgctt catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca
    50161 gccgcatcgc ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag
    50221 aaagtaccat cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta
    50281 tgatgattat cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa
    50341 aaaccaggcg aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt
    50401 actagaaata gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg
    50461 ctttttcccg ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc
    50521 aactttgatg ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag
    50581 atttttcggt gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa
    50641 cttcggagcg ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat
    50701 cttcaatttc acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg
    50761 tacaaaactt aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact
    50821 ttcttctaca cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc
    50881 tccttgacca gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt
    50941 aactgaatgt gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat
    51001 ttctttccct gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg
    51061 cgtttggcgt tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg
    51121 tgtgcctggt tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt
    51181 gatttctttt tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa
    51241 atcagcacct tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga
    51301 gtcgaagata tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa
    51361 tccgccacca gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc
    51421 gtatgcagcg cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa
    51481 tttttcttcc gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg
    51541 ataagctttc tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata
    51601 atcacgtttt gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg
    51661 ttatcatatc atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa
    51721 aaagccaaag tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc
    51781 gccatcgact tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc
    51841 tggattttct tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg
    51901 aacgatttcg ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac
    51961 agcggctttt aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt
    52021 gccttctaat tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg
    52081 taggtcaact tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac
    52141 catacgttcg atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc
    52201 tttttgtgta cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa
    52261 tgttacttcg atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct
    52321 tcctagagtt ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac
    52381 tgctggttga ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt
    52441 attgcggtca atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg
    52501 tgtaacgtca agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc
    52561 agcacccata gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc
    52621 tttacgtact gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc
    52681 gtcgatttct gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt
    52741 acgttctact aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa
    52801 atgcaatggt ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac
    52861 gccagataaa tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt
    52921 gtcttgagac aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat
    52981 tttgttatca aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa
    53041 gacaccgtct cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac
    53101 taagattttt tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg
    53161 ttcattgacg atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg
    53221 acgttgtgca tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc
    53281 taaatagtct tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg
    53341 tgtatatgat ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat
    53401 tgatgaaatc gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat
    53461 ttctccattt ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat
    53521 gatttttgct tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat
    53581 accgataatt ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat
    53641 ccgcacaatt attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg
    53701 tagccttctt gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca
    53761 gtttgtactg cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca
    53821 attccttctt cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc
    53881 aaacctgccc cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt
    53941 gatggaagta attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca
    54001 ttttttcctc gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc
    54061 atttcttcta actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa
    54121 atacccgaat cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt
    54181 tctttatcta gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc
    54241 agctgtttga ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat
    54301 tcacattacg agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt
    54361 tctcttcgat aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct
    54421 aacaaggcta ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc
    54481 atattctgca ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc
    54541 atcggagtga tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg
    54601 aactgatcaa gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa
    54661 accttttctt caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc
    54721 gtctggaaat atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc
    54781 atcagaggat ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga
    54841 cttcccatat attcgggaag agtgaacact tggctttcta cattccccat atctgttacg
    54901 acgatagcaa taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg
    54961 tcttttattt caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca
    55021 gcagattgct taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc
    55081 agcagcaaat catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga
    55141 tagcctaaag cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca
    55201 tcttctaatg ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac
    55261 attaatgttt tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt
    55321 aaaatgtcgt tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt
    55381 ctaagtgcta attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact
    55441 ccatttttta gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt
    55501 tatatcaata cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg
    55561 ataaaaaaca aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc
    55621 cccaaaaaag tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct
    55681 tcttgctcta attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga
    55741 tcatagaaat gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct
    55801 tctatttgat tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt
    55861 aatgggcgta aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa
    55921 ccgctagcac cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt
    55981 tgagattcat gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt
    56041 tccattgctt caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg
    56101 agtcttcctt gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac
    56161 aaagaatagt gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct
    56221 agtgtttgtc ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct
    56281 tctaaaaatt gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc
    56341 aataagcgat tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc
    56401 atcacttgaa gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta
    56461 ccattttgaa aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca
    56521 gataaagaag ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga
    56581 ctttccagca ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc
    56641 ccttctaaaa aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata
    56701 tgaatgtacg cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt
    56761 tctccttacg tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc
    56821 aactcattga aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc
    56881 ttattgtcat cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag
    56941 ctgaccttcg gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc
    57001 tccttaagtg ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg
    57061 tatttggcga acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac
    57121 actcatgatt cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa
    57181 tctctcactt caagagtttt aactagattc caattattat ttttttgtaa aaataaacag
    57241 tctcttaatc atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat
    57301 tatcttctat ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc
    57361 gtgaatttta aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa
    57421 tactgttgtt ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt
    57481 gtattcatct tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc
    57541 ataaaattca gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat
    57601 catagcccta taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa
    57661 aaatttactt gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc
    57721 gagctcttta ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt
    57781 tcatgagtaa agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg
    57841 tacttttgaa gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt
    57901 acttcgttac ttataataga acttagtctt atatagaagg catagctcac attagcttat
    57961 ttgtcctata aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat
    58021 cttcagcaat ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt
    58081 gggacataac gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat
    58141 atgatgacaa tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag
    58201 ttgacaaata ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata
    58261 tatttttgtt tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt
    58321 attataatta ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat
    58381 atgtctagtt tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat
    58441 ttatt
//
LOCUS       DACDCJ010000008        53774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDCJ010000008 DACDCJ010000000
VERSION     DACDCJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.008"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IV038_000492"
     CDS             11..733
                     /locus_tag="IV038_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8340738.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IV038_000493"
     CDS             791..2830
                     /locus_tag="IV038_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP8340739.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLLAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="IV038_000494"
     CDS             complement(2939..4090)
                     /locus_tag="IV038_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8340740.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="IV038_000495"
     CDS             complement(4244..4774)
                     /locus_tag="IV038_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340741.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="IV038_000496"
     CDS             complement(4873..5223)
                     /locus_tag="IV038_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP8340742.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IV038_000497"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IV038_000497"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP8340743.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IV038_000498"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IV038_000498"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP8340744.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="IV038_000499"
     CDS             complement(7626..8288)
                     /locus_tag="IV038_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8340745.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="IV038_000500"
     CDS             8472..10118
                     /locus_tag="IV038_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8340746.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..11191
                     /gene="uxuA"
                     /locus_tag="IV038_000501"
     CDS             10109..11191
                     /gene="uxuA"
                     /locus_tag="IV038_000501"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8340747.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11299..12852)
                     /locus_tag="IV038_000502"
     CDS             complement(11299..12852)
                     /locus_tag="IV038_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8340748.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13000..14288
                     /locus_tag="IV038_000503"
                     /pseudo
     CDS             13000..14288
                     /locus_tag="IV038_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14414..15493)
                     /locus_tag="IV038_000504"
     CDS             complement(14414..15493)
                     /locus_tag="IV038_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8340749.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15596..16036)
                     /locus_tag="IV038_000505"
     CDS             complement(15596..16036)
                     /locus_tag="IV038_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8340750.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16093..16503)
                     /locus_tag="IV038_000506"
     CDS             complement(16093..16503)
                     /locus_tag="IV038_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8340751.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16683..17117)
                     /locus_tag="IV038_000507"
     CDS             complement(16683..17117)
                     /locus_tag="IV038_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8340752.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17217..18065)
                     /locus_tag="IV038_000508"
     CDS             complement(17217..18065)
                     /locus_tag="IV038_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8340753.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18454..20220
                     /locus_tag="IV038_000509"
     CDS             18454..20220
                     /locus_tag="IV038_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP8340754.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20322..21581
                     /locus_tag="IV038_000510"
     CDS             20322..21581
                     /locus_tag="IV038_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8340755.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21728..23029
                     /locus_tag="IV038_000511"
     CDS             21728..23029
                     /locus_tag="IV038_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8340756.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23032..23883
                     /locus_tag="IV038_000512"
     CDS             23032..23883
                     /locus_tag="IV038_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8340757.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23896..24711
                     /locus_tag="IV038_000513"
     CDS             23896..24711
                     /locus_tag="IV038_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP8340758.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24862..26028
                     /locus_tag="IV038_000514"
     CDS             24862..26028
                     /locus_tag="IV038_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8340759.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26258..26905
                     /locus_tag="IV038_000515"
     CDS             26258..26905
                     /locus_tag="IV038_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP8340760.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26989..27933
                     /locus_tag="IV038_000516"
     CDS             26989..27933
                     /locus_tag="IV038_000516"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP8340761.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28068..29519
                     /locus_tag="IV038_000517"
     CDS             28068..29519
                     /locus_tag="IV038_000517"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP8340762.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29540..30376
                     /locus_tag="IV038_000518"
     CDS             29540..30376
                     /locus_tag="IV038_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8340763.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30376..31035
                     /locus_tag="IV038_000519"
     CDS             30376..31035
                     /locus_tag="IV038_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8340764.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31161..31850
                     /locus_tag="IV038_000520"
     CDS             31161..31850
                     /locus_tag="IV038_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340765.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31856..32812
                     /locus_tag="IV038_000521"
     CDS             31856..32812
                     /locus_tag="IV038_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP8340766.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32856..33368
                     /locus_tag="IV038_000522"
     CDS             32856..33368
                     /locus_tag="IV038_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340767.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33624..34172)
                     /locus_tag="IV038_000523"
     CDS             complement(33624..34172)
                     /locus_tag="IV038_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8340768.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34261..35442)
                     /locus_tag="IV038_000524"
     CDS             complement(34261..35442)
                     /locus_tag="IV038_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP8340769.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35678..37186)
                     /locus_tag="IV038_000525"
     CDS             complement(35678..37186)
                     /locus_tag="IV038_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP8340770.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37345..37536
                     /locus_tag="IV038_000526"
     CDS             37345..37536
                     /locus_tag="IV038_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340771.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37739..38657
                     /locus_tag="IV038_000527"
                     /pseudo
     CDS             37739..38657
                     /locus_tag="IV038_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
     gene            38650..39723
                     /locus_tag="IV038_000528"
     CDS             38650..39723
                     /locus_tag="IV038_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340772.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39871..40389
                     /locus_tag="IV038_000529"
     CDS             39871..40389
                     /locus_tag="IV038_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340773.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40370..40762
                     /locus_tag="IV038_000530"
     CDS             40370..40762
                     /locus_tag="IV038_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP8340774.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40838..41773
                     /locus_tag="IV038_000531"
     CDS             40838..41773
                     /locus_tag="IV038_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP8340775.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41906..42685
                     /locus_tag="IV038_000532"
     CDS             41906..42685
                     /locus_tag="IV038_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP8340776.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42678..42995
                     /locus_tag="IV038_000533"
     CDS             42678..42995
                     /locus_tag="IV038_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP8340777.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43239..44849
                     /locus_tag="IV038_000534"
     CDS             43239..44849
                     /locus_tag="IV038_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8340778.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45006..45854
                     /locus_tag="IV038_000535"
     CDS             45006..45854
                     /locus_tag="IV038_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8340779.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46085..46564
                     /locus_tag="IV038_000536"
     CDS             46085..46564
                     /locus_tag="IV038_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340780.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46597..48825
                     /locus_tag="IV038_000537"
     CDS             46597..48825
                     /locus_tag="IV038_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8340781.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            48954..50597
                     /locus_tag="IV038_000538"
     CDS             48954..50597
                     /locus_tag="IV038_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8340782.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50670..51215)
                     /locus_tag="IV038_000539"
     CDS             complement(50670..51215)
                     /locus_tag="IV038_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP8340783.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51238..51723)
                     /locus_tag="IV038_000540"
     CDS             complement(51238..51723)
                     /locus_tag="IV038_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8340784.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51713..52525)
                     /locus_tag="IV038_000541"
     CDS             complement(51713..52525)
                     /locus_tag="IV038_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP8340785.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52664..>53774)
                     /locus_tag="IV038_000542"
     CDS             complement(52664..>53774)
                     /locus_tag="IV038_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8340786.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17907 a         9784 c        10381 g        15702 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgcttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatataccag taggagaggt atggcccagg gagaggatca aagaactaaa
    10261 agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca atgtgcatga
    10321 tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata agcagacaat
    10381 ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc ctgtatttga
    10441 ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt tagcttttat
    10501 atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac attccagcaa
    10561 tgaatttgaa ttgccgggat gggaacctga gcgcttagct catatcaaat ccttgcttga
    10621 agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt ttctccaatc
    10681 aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag atgatccgcc
    10741 atactcgatt tttggattac ctcgaatcgt aaagaatcga gaagatttag attggttgtg
    10801 ccgcatagta gacagccctt ataacggaat cactctttgt acagggagta ttgcagaaga
    10861 tccagaaaat gatgtatatg ggattttagc agaattttgt caaagagatc gaatcccatt
    10921 tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg caccgcataa
    10981 atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg acaacggatt
    11041 taacgaatac atccgtcctg atcatggaag aatgatctgg aatgagacag gaagaccagg
    11101 atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat gggaagcaat
    11161 cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa
    11221 taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa gcattcggat
    11281 ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt gatccatgag
    11341 tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt ttttccagtc
    11401 ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag gcaattgttt
    11461 caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt gtattttgat
    11521 cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga tataaaaatc
    11581 acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat catacaaatg
    11641 tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg gtaacaagtg
    11701 ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga cttctttcaa
    11761 cacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt gattgaagtt
    11821 aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt
    11881 ttcaggtata aacaaatcaa atacagtaca aaaggactcc accatttctc ttgaataaag
    11941 aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg tttcttccga
    12001 ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt gttcgcgaca
    12061 aattggcaga ttaggtcgca aaatatctac agagggatca ataaaaaagt caatatattc
    12121 atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat tctctttaca
    12181 tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt caagtacttc
    12241 ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat cataaaattc
    12301 tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt gaatttgtac
    12361 ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca aaccgtacaa
    12421 tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac tatagtttgt
    12481 ttgctgactt aaactgataa tcgaatttgt ctgacagctg aacaaagaag tcaagatctg
    12541 ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt aagccggatc
    12601 aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca gttctgttag
    12661 attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat ttgattcaga
    12721 catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt cagataggaa
    12781 aagaaaaata tctaattttg tattgctttt tttcttcaag agaaacgttt taatcgcttc
    12841 ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga aaatattact
    12901 gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc tctactatct
    12961 tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc aaccatctta
    13021 atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt ccacacagag
    13081 gagatagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg aattaccttt
    13141 caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg acaaacagcg
    13201 accatcaaag caaaaattga ctattcagga gacttagcac ctatactaca atcagctgtt
    13261 acatttgaga atgttgatcc cgctttgtcg attggctatg acgagaacac tttgactatc
    13321 caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt gttaaaacca
    13381 gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcatca tttaacgccg
    13441 tatagtcagc ggcagacaat tgagcaagat tcagctatcg actcaggagg agatcttgtt
    13501 gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc tacagaaaat
    13561 ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac agaaactcaa
    13621 ccagagccag acaatgaatc aaaacctgaa ataacccctg gagaaaaacc agatgtagac
    13681 ccagaagaaa aaccagatgt gactccagaa ccagatacgg attctggaaa ccaaacagta
    13741 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagcc
    13801 ccagaagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta
    13861 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat
    13921 ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata
    13981 ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca
    14041 aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat
    14101 agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa
    14161 cgaaaaataa acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg
    14221 gaatcattct attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat
    14281 ctctctaaca tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga
    14341 aaatgacccc aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt
    14401 cttcatattt attttaaggg atatctgttt cttttggaag atggttcggg atctttttca
    14461 ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc
    14521 caaagaaccg ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt
    14581 tgctggacat caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg
    14641 taatcaggat ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag
    14701 gaatacaaga aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg
    14761 ctaaactcag cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat
    14821 ttaaaagagc gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat
    14881 cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg
    14941 agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag
    15001 caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat
    15061 tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag
    15121 gatctgcatc ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg
    15181 tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca
    15241 gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt
    15301 gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac
    15361 ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt
    15421 cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa
    15481 acacgttatt cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct
    15541 aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc
    15601 cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat
    15661 ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt
    15721 gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg
    15781 tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg
    15841 taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc
    15901 ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag
    15961 tgtagtgtct aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta
    16021 aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt
    16081 acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc
    16141 ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga
    16201 aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt cactttctga
    16261 ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat
    16321 caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt
    16381 ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg
    16441 attatgcact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt
    16501 cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg
    16561 gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc
    16621 tttctcgaat agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa
    16681 cattacatat atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc
    16741 tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag
    16801 tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt
    16861 gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta
    16921 ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag
    16981 gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca
    17041 tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt
    17101 tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt
    17161 ttattatacc agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat
    17221 tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca
    17281 aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc
    17341 acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg
    17401 gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca
    17461 ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt
    17521 gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga
    17581 ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa
    17641 tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat
    17701 aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct
    17761 gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa
    17821 agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata
    17881 ttcaacacta aaataactat agctcggtca aagcctaaag cagcttccag taatattcct
    17941 agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact
    18001 cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct
    18061 ttcatttatt cacttcctta attcattaat aatactctta tcttattata acttactttt
    18121 caccttaagt taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat
    18181 tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg
    18241 agaaattgat cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac
    18301 ttttaaaagt tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa
    18361 aaactgttac cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg
    18421 atgacgtcat attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga
    18481 ccggaaagtg aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact
    18541 aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact
    18601 gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat
    18661 tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt
    18721 ggggaagata acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat
    18781 gtgattactg aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc
    18841 actattccag aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca
    18901 aacggggaca aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac
    18961 cgagaagact ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg
    19021 gaccttggca tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa
    19081 tacgatacga ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa
    19141 aacttgattg atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat
    19201 catatgggtg tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc
    19261 tataaagact ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta
    19321 ccagaaactg ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa
    19381 ctaaatacag caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata
    19441 aaagaattcg atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt
    19501 tggaaaaaat tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag
    19561 atttggcatt cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat
    19621 gcatttacag attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg
    19681 gtcagtggaa tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc
    19741 aacttattag attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa
    19801 ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac
    19861 gggacagaaa tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg
    19921 gagaaggagg aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg
    19981 aaacagttgt ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt
    20041 gaagataagc tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat
    20101 cagtcaaaag aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt
    20161 cagttgttag aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga
    20221 aaactgttac cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat
    20281 aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga
    20341 aacagttggg tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca
    20401 atggtaactc ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg
    20461 attctggata taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg
    20521 tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg
    20581 ggcctgctgg aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag
    20641 gacagcaagg acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag
    20701 acaaagccca agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc
    20761 ttgttttgta ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg
    20821 agaatctatc caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat
    20881 ttttagctaa atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct
    20941 atgtatttgg tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg
    21001 tagaaggaat ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag
    21061 acataaaaag tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa
    21121 tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag
    21181 ccaaaattcc tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat
    21241 gggtagtcag caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa
    21301 cgaatcagga aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac
    21361 aagctaggga aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat
    21421 atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag
    21481 aaaacttgat gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag
    21541 cagtaaaaac aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca
    21601 tttttataaa aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt
    21661 atcttgctct tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg
    21721 cattcagatg cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc
    21781 acttattcca gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt
    21841 cgccatattc gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg
    21901 attggttact ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg
    21961 cactttgcaa ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga
    22021 cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga
    22081 agtcatacat aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct
    22141 tctcatgatt tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa
    22201 ttatgacttc aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt
    22261 ttttagtatc ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac
    22321 cgtgatttgg actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt
    22381 tgttgctcac cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc
    22441 atgggcggtc ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt
    22501 aggtgccatc aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag
    22561 tgcaatctct tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg
    22621 ctggttagga tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga
    22681 agacttatat gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat
    22741 cacgcttcct gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa
    22801 tttcaataat ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg
    22861 aggagctggt tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc
    22921 tccgcagtat tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt
    22981 gtcattgctg gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa
    23041 tttcattcga gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt
    23101 ttaatactgt cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag
    23161 tcaggaaata ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt
    23221 actcggctat tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct
    23281 gtcctaacaa tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc
    23341 tatcgtttta tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg
    23401 actatggcag cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag
    23461 tactggttct tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg
    23521 aaaggatact ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca
    23581 gggcatttca ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt
    23641 caagcattat gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt
    23701 cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca
    23761 aaccaaagag tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta
    23821 ttattcttct ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga
    23881 taggagcaga gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca
    23941 actcaagtgt ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta
    24001 tttttagttt ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg
    24061 attccattag aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa
    24121 ttttctgaac ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg
    24181 acccctgaaa ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact
    24241 tttattgatt ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc
    24301 cgctatacga tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat
    24361 ttccaacgat ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct
    24421 tatagtttaa gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct
    24481 tcctttttct tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag
    24541 agtgcaaatt tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg
    24601 tttatccact ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg
    24661 cttttatggg tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa
    24721 gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt
    24781 ctgaaaaaat taaacttctt agttgattct tttctttttt agtattaacg taatagggta
    24841 gtttttttaa ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt
    24901 tctttctcgc tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat
    24961 acggacactc ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt
    25021 acaagtaacg cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag
    25081 ggaagtatcc aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga
    25141 aaagattatc taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa
    25201 gatatccatg cgcattatga cttaggtaac gacttttata aactttggct agatccaact
    25261 ttgacttatt cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc
    25321 aataaagtcc atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat
    25381 atcggttgtg gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca
    25441 actgggatca cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa
    25501 catttagaag ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg
    25561 tttgatcata tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt
    25621 tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc
    25681 agccgacaac aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt
    25741 tatgtccctg gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt
    25801 gatttggaaa gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt
    25861 catcaagtcc aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat
    25921 ctatatttac aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat
    25981 ttgcttgttc aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag
    26041 tactgtttaa agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt
    26101 tttgtctaac ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa
    26161 taaaagtttg gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt
    26221 aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac
    26281 tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc
    26341 tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa
    26401 agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga
    26461 tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc
    26521 aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt
    26581 cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga
    26641 gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct
    26701 ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg
    26761 aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt
    26821 caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt
    26881 actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt
    26941 tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa
    27001 acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc
    27061 atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag
    27121 agctgtacga tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta
    27181 gggagtatca attctatggg acttccaaac ttaggttttt cttattatct agagtatgct
    27241 cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg
    27301 atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg
    27361 atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat
    27421 tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga
    27481 atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat
    27541 cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat
    27601 acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag
    27661 tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa
    27721 atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta
    27781 tgtggcgcaa gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt
    27841 tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac
    27901 gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct
    27961 tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat
    28021 gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa
    28081 cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata
    28141 tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat
    28201 tcgagagtaa ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta
    28261 tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat
    28321 ctcgtttatt tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag
    28381 cgataggtct agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta
    28441 cagacccagt catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg
    28501 gatcaagtgg aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta
    28561 gtgatggcgg tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac
    28621 cttcgcgagg gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta
    28681 ttgatttcgg cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa
    28741 gtatccattc cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa
    28801 aaaaacggtt gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag
    28861 aggcagtact tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga
    28921 cagagattcc ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg
    28981 gcgcagaaac agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa
    29041 aagaagaact tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg
    29101 ctacttacat cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac
    29161 atcaagaata cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg
    29221 aagatttgca aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag
    29281 tagaaggttt agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata
    29341 cacagttggc taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag
    29401 aaaacatgcc tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc
    29461 aaatcggcta tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc
    29521 agaaacaagg agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt
    29581 ttttatttta agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa
    29641 tgtagaaaaa aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta
    29701 ttataatgat caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa
    29761 acggttaaat gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt
    29821 ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa
    29881 aaaattctcc ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa
    29941 agatgattca ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc
    30001 caatactagc tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa
    30061 tgccacgaat gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa
    30121 tgattattat ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact
    30181 ggagaacgtt tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc
    30241 tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa
    30301 aatatctgaa aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga
    30361 gtcggtgagt gagtgatgta ctttcctcta attgattttt ccctcatgtg gacttcactt
    30421 cctttcgtac tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta
    30481 ggaaatctgg tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat
    30541 gtatttattc gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt
    30601 ctgttatatt ttgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt
    30661 acgattacca gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct
    30721 tcaggtcagt gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat
    30781 atcgttttgc cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat
    30841 ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca
    30901 aagatcatag gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt
    30961 tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg
    31021 catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt
    31081 gtttcttgta tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa
    31141 tatgttcgga ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat
    31201 accaatattg catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc
    31261 aaggaattgt agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat
    31321 gctatgagct gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg
    31381 gacattataa ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc
    31441 ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct
    31501 ctggtacatt tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag
    31561 ctttgacaaa ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg
    31621 agcgaggcgt aaaatatttt ccaacagatc aattgtacaa agaattgaaa agacgttcag
    31681 aaaatcaaga tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta
    31741 gtcaggcaat caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt
    31801 tgaggcaaca acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta
    31861 ctatccttat ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg
    31921 aggattgctc tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct
    31981 taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc
    32041 ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa
    32101 tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc
    32161 acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa
    32221 acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg
    32281 gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag
    32341 ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg
    32401 atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc
    32461 tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa
    32521 acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc
    32581 aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg
    32641 aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa
    32701 aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga
    32761 ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa
    32821 tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag
    32881 aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag
    32941 gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg
    33001 aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag
    33061 tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc
    33121 cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa
    33181 aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta
    33241 tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt
    33301 atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca
    33361 tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca
    33421 atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat
    33481 ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt
    33541 tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat
    33601 cctacgagta ttattcgata catttatatc aataatcctt tttttatttt atctgcagct
    33661 tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc
    33721 aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct
    33781 ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg
    33841 actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa
    33901 caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga
    33961 gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca
    34021 gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa
    34081 gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca
    34141 gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa
    34201 gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata
    34261 ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa
    34321 aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc
    34381 accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat
    34441 tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa
    34501 ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg
    34561 gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat
    34621 gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag
    34681 catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg
    34741 gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac
    34801 atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt
    34861 gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt
    34921 agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt
    34981 ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga
    35041 ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa
    35101 tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat
    35161 caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc
    35221 aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac
    35281 cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat
    35341 cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc
    35401 taatgtgaca aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca
    35461 aattccgttt tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat
    35521 tactttttta gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa
    35581 ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct
    35641 aatagtttcg ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg
    35701 aagggtagac aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat
    35761 ttttattgac catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat
    35821 aagcaacttg agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat
    35881 tgatgcttag tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt
    35941 caagaactaa tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg
    36001 catttaagat aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga
    36061 cttcatctgg taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt
    36121 gaatcttatc tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac
    36181 cgacttcttc cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt
    36241 tgaagtcgca ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga
    36301 caacggtatc atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt
    36361 gataaacaga ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct
    36421 caacatcccg attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga
    36481 tcatattgac atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt
    36541 tcaacggaac attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt
    36601 tttggctttc atgatacagt tggttcaagg aagtagcgag ctttccactt cctgggccag
    36661 gagctgtcac tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat
    36721 cttctgagac gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga
    36781 ccttgatgtt tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat
    36841 agcgagtgat aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc
    36901 gcaagatatc catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat
    36961 ctccagcata gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga
    37021 ttttcgcatc ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactagct
    37081 tcccaccaaa ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg
    37141 actgctcttc aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt
    37201 aagtactaaa gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct
    37261 caaaaaaaca aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat
    37321 agtttttgat aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa
    37381 cactatatcc gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa
    37441 caattgacgt ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc
    37501 attcatttca caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat
    37561 tcgattctgt cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt
    37621 tggtgcagcc tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa
    37681 atggtactat tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat
    37741 gaaagagcca gatacaaatg caaattatga gccagacgaa ctagaggaga cgaatgcgga
    37801 tcaggataca ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa
    37861 tctttcccaa ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat
    37921 ccctgagatc taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa
    37981 tgaaaatcac gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga
    38041 atttcctttt atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg
    38101 cacttattac catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt
    38161 tttgcctaag atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga
    38221 caaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt
    38281 tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat
    38341 tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac
    38401 tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc
    38461 aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa
    38521 aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc
    38581 atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag
    38641 gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa
    38701 gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca
    38761 gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg
    38821 aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta
    38881 gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa
    38941 ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa
    39001 tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg
    39061 atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca
    39121 aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga
    39181 caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa
    39241 tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg
    39301 gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg
    39361 atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt
    39421 aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa
    39481 ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat
    39541 atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg
    39601 ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga
    39661 tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc
    39721 tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg
    39781 gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa
    39841 cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc
    39901 ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag
    39961 ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt
    40021 gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc
    40081 ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa
    40141 acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac
    40201 ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat
    40261 taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt
    40321 gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat
    40381 aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac
    40441 ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat
    40501 cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga
    40561 tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac
    40621 ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga
    40681 cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat
    40741 tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata
    40801 attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg
    40861 aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt
    40921 cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga
    40981 ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga
    41041 gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat
    41101 gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc
    41161 atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat
    41221 cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact
    41281 ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga
    41341 agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc
    41401 aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg
    41461 agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc
    41521 aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga
    41581 tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt
    41641 tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca
    41701 gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta
    41761 ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg
    41821 tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt
    41881 tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc
    41941 tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt
    42001 attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt
    42061 ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac
    42121 gacattattg atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc
    42181 cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga
    42241 tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa
    42301 ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa
    42361 ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat
    42421 gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt
    42481 ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc
    42541 agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa
    42601 agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga
    42661 acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca
    42721 tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat
    42781 cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta
    42841 aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa
    42901 tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag
    42961 gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga
    43021 gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct
    43081 ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt
    43141 ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa
    43201 gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag
    43261 gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga
    43321 ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata
    43381 atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg
    43441 cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga
    43501 tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt
    43561 atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac
    43621 tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag
    43681 gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg
    43741 ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa
    43801 ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga
    43861 ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga
    43921 tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa
    43981 cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa
    44041 cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct
    44101 tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag
    44161 gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac
    44221 aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat
    44281 tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg
    44341 aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga
    44401 tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag
    44461 aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg
    44521 ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta
    44581 tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt
    44641 tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc
    44701 gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg
    44761 gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg
    44821 ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa
    44881 aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat
    44941 ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta
    45001 aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga
    45061 accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg
    45121 aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc
    45181 ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga
    45241 aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca
    45301 cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga
    45361 tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc
    45421 agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc
    45481 tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc
    45541 tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt
    45601 tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta
    45661 gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag
    45721 aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa
    45781 ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca
    45841 tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt
    45901 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
    45961 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
    46021 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
    46081 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
    46141 taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac tgggccttgc
    46201 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
    46261 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
    46321 agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
    46381 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
    46441 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
    46501 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
    46561 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
    46621 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
    46681 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
    46741 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
    46801 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
    46861 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
    46921 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
    46981 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
    47041 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
    47101 cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat ccgagatgcc
    47161 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
    47221 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
    47281 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
    47341 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
    47401 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
    47461 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
    47521 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
    47581 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
    47641 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa aggaagaacc
    47701 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
    47761 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
    47821 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
    47881 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
    47941 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
    48001 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
    48061 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
    48121 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
    48181 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
    48241 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
    48301 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
    48361 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
    48421 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
    48481 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
    48541 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
    48601 ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
    48661 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
    48721 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
    48781 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
    48841 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
    48901 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
    48961 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
    49021 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
    49081 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
    49141 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
    49201 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
    49261 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
    49321 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
    49381 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
    49441 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
    49501 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
    49561 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
    49621 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
    49681 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
    49741 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
    49801 aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc gcttggcaaa
    49861 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
    49921 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
    49981 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
    50041 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
    50101 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
    50161 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
    50221 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
    50281 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
    50341 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
    50401 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
    50461 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
    50521 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
    50581 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
    50641 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
    50701 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
    50761 acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat atcgataccg
    50821 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
    50881 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
    50941 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
    51001 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
    51061 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
    51121 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
    51181 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
    51241 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
    51301 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
    51361 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
    51421 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
    51481 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
    51541 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
    51601 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
    51661 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
    51721 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
    51781 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
    51841 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
    51901 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
    51961 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
    52021 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
    52081 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
    52141 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
    52201 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
    52261 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
    52321 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
    52381 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
    52441 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
    52501 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
    52561 tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa
    52621 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
    52681 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
    52741 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
    52801 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
    52861 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
    52921 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
    52981 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
    53041 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
    53101 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
    53161 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
    53221 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
    53281 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
    53341 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
    53401 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
    53461 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
    53521 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
    53581 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
    53641 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
    53701 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
    53761 aataaagaat agga
//
LOCUS       DACDCJ010000009        50998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDCJ010000009 DACDCJ010000000
VERSION     DACDCJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.009"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            37..882
                     /locus_tag="IV038_000543"
     CDS             37..882
                     /locus_tag="IV038_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8340787.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1163..2383
                     /locus_tag="IV038_000544"
     CDS             1163..2383
                     /locus_tag="IV038_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP8340788.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2380..5100
                     /locus_tag="IV038_000545"
     CDS             2380..5100
                     /locus_tag="IV038_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8340789.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5084..6028
                     /locus_tag="IV038_000546"
     CDS             5084..6028
                     /locus_tag="IV038_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8340790.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6097..7107)
                     /locus_tag="IV038_000547"
     CDS             complement(6097..7107)
                     /locus_tag="IV038_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8340791.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7287..7631)
                     /locus_tag="IV038_000548"
     CDS             complement(7287..7631)
                     /locus_tag="IV038_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP8340792.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7624..8058)
                     /locus_tag="IV038_000549"
     CDS             complement(7624..8058)
                     /locus_tag="IV038_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP8340793.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8196..8933
                     /locus_tag="IV038_000550"
     CDS             8196..8933
                     /locus_tag="IV038_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340794.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8926..10137
                     /locus_tag="IV038_000551"
     CDS             8926..10137
                     /locus_tag="IV038_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340795.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10344..11123
                     /locus_tag="IV038_000552"
     CDS             10344..11123
                     /locus_tag="IV038_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP8340796.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11193..11855
                     /gene="trmB"
                     /locus_tag="IV038_000553"
     CDS             11193..11855
                     /gene="trmB"
                     /locus_tag="IV038_000553"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP8340797.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11953..13707)
                     /gene="ade"
                     /locus_tag="IV038_000554"
     CDS             complement(11953..13707)
                     /gene="ade"
                     /locus_tag="IV038_000554"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP8340798.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13839..14768
                     /gene="cpaF"
                     /locus_tag="IV038_000555"
     CDS             13839..14768
                     /gene="cpaF"
                     /locus_tag="IV038_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8340799.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14725..15774
                     /locus_tag="IV038_000556"
     CDS             14725..15774
                     /locus_tag="IV038_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP8340800.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15771..16049
                     /locus_tag="IV038_000557"
     CDS             15771..16049
                     /locus_tag="IV038_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP8340801.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16024..16494
                     /locus_tag="IV038_000558"
     CDS             16024..16494
                     /locus_tag="IV038_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP8340802.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16511..16813
                     /locus_tag="IV038_000559"
     CDS             16511..16813
                     /locus_tag="IV038_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340803.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16830..17201
                     /locus_tag="IV038_000560"
     CDS             16830..17201
                     /locus_tag="IV038_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP8340804.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17173..17532
                     /locus_tag="IV038_000561"
     CDS             17173..17532
                     /locus_tag="IV038_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340805.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17704..18711
                     /locus_tag="IV038_000562"
     CDS             17704..18711
                     /locus_tag="IV038_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8340806.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18736..19920
                     /locus_tag="IV038_000563"
     CDS             18736..19920
                     /locus_tag="IV038_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP8340807.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20357..21940)
                     /locus_tag="IV038_000564"
     CDS             complement(20357..21940)
                     /locus_tag="IV038_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8340808.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22518..22661)
                     /locus_tag="IV038_000565"
     CDS             complement(22518..22661)
                     /locus_tag="IV038_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP8340809.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22884..23363)
                     /locus_tag="IV038_000566"
     CDS             complement(22884..23363)
                     /locus_tag="IV038_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8340810.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23436..23720)
                     /locus_tag="IV038_000567"
     CDS             complement(23436..23720)
                     /locus_tag="IV038_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340811.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23798..23992)
                     /gene="ssrS"
                     /locus_tag="IV038_000568"
     ncRNA           complement(23798..23992)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IV038_000568"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24064..25350)
                     /locus_tag="IV038_000569"
     CDS             complement(24064..25350)
                     /locus_tag="IV038_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP8340812.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25488..26159
                     /locus_tag="IV038_000570"
     CDS             25488..26159
                     /locus_tag="IV038_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP8340813.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26125..26613
                     /locus_tag="IV038_000571"
     CDS             26125..26613
                     /locus_tag="IV038_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP8340814.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26626..27573
                     /gene="prmA"
                     /locus_tag="IV038_000572"
     CDS             26626..27573
                     /gene="prmA"
                     /locus_tag="IV038_000572"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP8340815.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27574..28338
                     /locus_tag="IV038_000573"
     CDS             27574..28338
                     /locus_tag="IV038_000573"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP8340816.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28571..30784
                     /locus_tag="IV038_000574"
     CDS             28571..30784
                     /locus_tag="IV038_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP8340817.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30795..31241
                     /locus_tag="IV038_000575"
     CDS             30795..31241
                     /locus_tag="IV038_000575"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP8340818.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31412..32671
                     /locus_tag="IV038_000576"
     CDS             31412..32671
                     /locus_tag="IV038_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP8340819.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32760..33707)
                     /locus_tag="IV038_000577"
     CDS             complement(32760..33707)
                     /locus_tag="IV038_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8340820.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFRETLTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34101..35393
                     /locus_tag="IV038_000578"
     CDS             34101..35393
                     /locus_tag="IV038_000578"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP8340821.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35408..37177
                     /gene="aspS"
                     /locus_tag="IV038_000579"
     CDS             35408..37177
                     /gene="aspS"
                     /locus_tag="IV038_000579"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP8340822.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37286..38161
                     /locus_tag="IV038_000580"
     CDS             37286..38161
                     /locus_tag="IV038_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8340823.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38427..40310
                     /locus_tag="IV038_000581"
     CDS             38427..40310
                     /locus_tag="IV038_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8340824.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40385..40669)
                     /locus_tag="IV038_000582"
     CDS             complement(40385..40669)
                     /locus_tag="IV038_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8340825.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40659..41393)
                     /locus_tag="IV038_000583"
     CDS             complement(40659..41393)
                     /locus_tag="IV038_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP8340826.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41522..42145
                     /locus_tag="IV038_000584"
     CDS             41522..42145
                     /locus_tag="IV038_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP8340827.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42337..43458
                     /locus_tag="IV038_000585"
     CDS             42337..43458
                     /locus_tag="IV038_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP8340828.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43465..46593
                     /locus_tag="IV038_000586"
     CDS             43465..46593
                     /locus_tag="IV038_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP8340829.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46612..49806
                     /locus_tag="IV038_000587"
     CDS             46612..49806
                     /locus_tag="IV038_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8340830.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49952..50659)
                     /locus_tag="IV038_000588"
     CDS             complement(49952..50659)
                     /locus_tag="IV038_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8340831.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16685 a         8927 c        10604 g        14782 t
ORIGIN      
        1 aataagcagt gaatttataa tcaacgtata ggagacatgg atgaaatgaa actaattggt
       61 atagatttag acggtactct tttgaattct gaccagagca ttagtcaaaa aaatgcaaga
      121 acaatgaaac atttaccgga agactgtttt ccattcattt gttctggaag agaagtagaa
      181 gacattaata atattctcaa aaaatctggt ttaaggatac cagcagtagg gttaaatggt
      241 tctgttggat ttgatggaga aaaaaagata tttgagtctt ccttcgataa aaatgatgta
      301 aaaaaaattg attctgtcct atcgaaattt ccaaccaaaa tatatacaaa tctcggaagt
      361 tatgaatctc agaattataa aaataaaatg aaaaaaattt ttcacgaggt agggagtgaa
      421 ttttctatca aagagctaaa ctatgagctt gagtatgaaa aatctatttg ttcaatccct
      481 tataataata ttgaggaaat tatagaaaag gatgctatca gcatttacaa attttttatt
      541 tttattccta atcgtcaatt gaaagctaat atatttaacc atttgaaaaa gatgctaaat
      601 gtgtctgtta ctgaatcggc acctgtaaat attgaaatcg tacctcaaaa tgtatctaaa
      661 ggtgtggtgt ttgatcgttt ggaatcaatc tacaatttaa agaaaccttt gagaatcgcg
      721 atcggagata gtttaaatga cttatctatg tttgaaagtg cagatattag ttttgcaatg
      781 ggaaatagtc atcaagaaat catgaaaaaa gctgattatg tcactgtttc aaatgataaa
      841 gacggagtat ctcaagcatt tgaaagaatt cttaccatat aaactaatta aggtagtata
      901 gaacatagaa ttctaaatac ggaaaaatgg agcataaatt ccagtacaaa tagtacttct
      961 tcagtgtcaa acgaagcgaa tataacatag atatttagga ttgtgacaaa aatcatgtca
     1021 caatcttttt ttcgaataaa cttatttttt gaagtcggcc atttatcgta atttctttgc
     1081 ataatacgaa caaattttcg gttattgatg aaaaaaaaca ttgaaaatgg tatgataaaa
     1141 gaaaaaaagg aggaaatttt taatggtgaa atttatccat gcggcagatt tacatatgga
     1201 tcggtcattt gaaggactga caactcttga taaaactgta caggaaaaac tattgaagac
     1261 gaatctgacc gttttatcaa atatcattga acaggcaata ataaataacg ttgattttgt
     1321 tttacttgca ggagataatt ttcatcaaaa tcgtccttca ttaaaaatac agaaacattt
     1381 ttcagaacag atgaaacggt tagaaaagaa ccatatacct gtatttataa tattcggaaa
     1441 tcatgatttc tatcaaaaag agcggtattg gttctccttt cctaaaaatg tccatttatt
     1501 tacatccgaa acggtagaga caaaaaaaat aacgataaaa agtggagaaa ctgtctctct
     1561 cagtggattt agttatcgtc aaccgtggat tcaaaaagac aaagtgatgg aatttccttc
     1621 tagagaactc acagattacc atattgggtt gtaccacggg gaacctggcg tctctcaaaa
     1681 aggaaactat gctcctttcc aaccctcaaa gatgcaagaa aagggatacg attattgggc
     1741 tttagggcat atccatgtgc caacggtctt aaatgaaaag caaacaattg tttatccagg
     1801 tgcgcctcaa ggtcatacta aaaaggaaca agcttctaca tctattctac tagttgaatt
     1861 aagtaaagga agttgccaaa tacatccgat taaagtagca gaggtctatt ggaagacaaa
     1921 ggaaatatca ttgaggaaag caagaacgac aaaagaggtc attgttcaca ttcgtcagca
     1981 gctttcaaaa gtagaggatg gatttagttt aattgagata aaattaaaag attatgacca
     2041 tctcaataca gatgttttgg aacggataca atcaggagag ctattagact atttgttaga
     2101 ggaattctca gataggataa atgatttctt tatttggcgt atttctctca tagagaatac
     2161 tttttatacg aagaccccac ttgctgcttc tgctaaattg atggaacagt tatttcaata
     2221 ctatcagaca cctcaagatt tccaacaaat tttaaatgaa gtatatagtc atcaagaagc
     2281 ggctagaata ttgagcgaac tgccggaata tcaagaagaa acacttgaaa aagccaagca
     2341 gctgcttaat caagattttt tatttgagga ggatcaggaa tgaagatttt aaggatcgag
     2401 attgctgctt ttggaaaatg gcgtcaaaaa agttttgact tttattcagg caatcagctg
     2461 atctatgggg gaaacgaagc aggaaaatca acgatttatc aatttattca agcaatatta
     2521 tttggtttcc cttcaaaagg ccgtaaaaaa aaggattata caccaaaaga cggctcagcc
     2581 tatggcggca aaatatggct taaacatcct gtatatggtg aatttgctgt cgagcgctac
     2641 aagcagcaaa atcgcggaaa atcgaaggtg tggctggggg atcaagtggg aagtgatgag
     2701 ttactagaaa aactgatcgc acctttgacg aaagagttat tccagcaggt atttactttc
     2761 cagcaagagc agttgatgga actagatcat ttgagagaaa aagagctaca tgatgcgctg
     2821 atttctttag gcatcacggg cagttctcaa gtattccaaa agcggcaaga atattatcag
     2881 aaagcacaac aattattcaa gaaaaaaggc caaaaattac taatcaatca aaaattaaat
     2941 gaatggaaag agctgcaaga aaaaattcat caaaaacaag aacaggaagc ttattttggc
     3001 aagttgatcc aacaagaaca ggaatacgag tcgcaacagc agcagttatt ggcacaatta
     3061 aaagaggcga acaaacaatt ttcattagcc agacagcaag aacttaattt ttctttatat
     3121 gaagaatggc aatctctcaa aaaaaacagc actacaggtc ttcctgatga gcaacttctt
     3181 ttagatctgg agtctttctc caaacggtat cagcaaacaa atgagcgttt ggaagaatta
     3241 gaagcaacac tggaaaagca tagcgggatg gatcagcagt cagccagata ttatttttat
     3301 ttagagcagg agcaggaact aaaagatctc cagcaacttc aatttacagc ggaacaatta
     3361 aacgaagaat ggcaaagatt acaaattgag actagagaaa caaattcaga attgaagatt
     3421 ttggaacaaa ggtggcattg gcagaaaaat ccgttaccgg aagaattttt atatgaagaa
     3481 gaatggaagc atcttctggc tgaagaaaga aggattgctg atctagaaag ccagatttct
     3541 gtgcgtctcc aaatagctaa agaacagcaa gagatgattg aagcagaagt gaatgacttg
     3601 gaagagacct atccagcatt attacaaaat cagtcagaga tagcaccagt ctcttcatct
     3661 aaaccgtata tgctgatggc tggcggagca ataatcattg caggtatatt tgtaccaatg
     3721 cccttgaaag tgatcctttt actaattggt gcaggcatga ttggctattt cttctacaaa
     3781 aggcaaccaa aaattaaaac taaaaatgat ttctctgaga taaaaggaat gtggcaagaa
     3841 aaactaggac aacttgatct agccttagct caattagctg aagcagaaga aaaacaaaag
     3901 agaactggag aagaaaaaca gattcttcag caagaagtat atcgttttgc ggatcatcgg
     3961 cacttaggaa agatggatac gctcaaactc atgaaagaat atggacagga agtcatagat
     4021 tatcagaaat tatatggcgt atttgagcgg aaacataaaa gagaacagga aatacaagaa
     4081 aaactacgac aaatcgacca aaaatttgag tttgttaaag aatggttacc gatacaagat
     4141 aagacaattt cagaaaaaat ggtattgctt tcccgtttta tacaagagat ggaacagatg
     4201 aaatttgcca gaagctatca gcaaaatact ttattgaagc aagaaatcaa tcaattgaaa
     4261 aagaaacaaa aagtatggat acaagaatac agcgaacaat tgactcaagc aggcttatct
     4321 tatccttcgg aagtacctgt ctttttacaa aaagccaaac aagccgaaca gcagatgaaa
     4381 agatacaatg aattatcgga aatgcttact ccactatttc ctgaagagct gacaaaagag
     4441 aaattatctg taagactgcg ccaactgaaa gaaaagcaat cccaactcca gcaagaagaa
     4501 aaagaagcaa gtgaaaatcg gcaacgactc caacttcaag tagaagagtt gcaaagagac
     4561 ggcacattgg acgaacttta tcaacaagct agccggcaaa aagcaaaatt acaggaatta
     4621 ctggaggaat ggggagcact tgaaatcgcc gggacattct taggtgatct ttcaacagag
     4681 atgagtgaaa gacaattacc ccagttgtta agagcagcag gtgattattt aggtatttta
     4741 acaaaaggaa attatcagaa gatattactg aaaaatgata cactgacttt aacagacgga
     4801 tatacagaat ttcccatcta tgagttatct acaggtacga aagatcagct gattatggcg
     4861 atgagatttg cttacctctc attagaagct aaccgttcga tatgcccgat catcattgat
     4921 gatggctggc ttcattatga tcatcagcga aagtatcaat tagctgaact tttagctaaa
     4981 tttggagaaa agtatcaaat aatttgtttt tcttctgata aagaaatggt aagctattac
     5041 catgagatga accaaatcgt aaaacaatta gaaggagcaa ataatgaaaa aaattcgtga
     5101 tttagtaatc gatgagcagt ttgaacaatt tgtcctgatc aaaagtgcag atgtaagaat
     5161 cgctaaaaac ggaaaaaaat ttattgcttt tactttccaa gatacttcag gtactatcga
     5221 tggtaaatac tgggatgctt cggaggagga aatcaataaa tttacagccg gcacagtcgt
     5281 ttttctaaac ggtaaaagag aggtttatca aggaaaccct caagtaaaga ttcttcatat
     5341 gcgcttaaca agggtagatg agccaggaga gcctagttta tacatggaaa gagccccttt
     5401 gaagaaagaa gaaatggaag aagaaatcaa tcaattgttg tttgagatca ccaatgcgca
     5461 ttggaatcgt attgttcgct atctacttaa taaatatcaa aaacaatttt ttgagtttcc
     5521 ggcagcaaaa agaaaccatc atgcatttgc cagtggatta gcttatcata ctttgacaat
     5581 gcttcactta gggaaatcaa tcgccgatga atatcctgag ctgaatcgag cattgttgta
     5641 tgctggtatc attttgcatg atttaggaaa ggtgattgaa ctttccggtc ctatgtcgac
     5701 agaatatacg cttgcaggaa atttgatcgg tcatctggta ttgattgacg aagaaataac
     5761 taaagcctgt cttgcgttga aaatcagcga agctgacgaa gatgttctcg tattacgtca
     5821 tatggtttta gcacaccatg gcttattaga atatggctca cctgtacgtc caagaataat
     5881 ggaagcagaa atattacatc aaatcgacaa tatcgatgct tcgatgcaga tgatgttgac
     5941 ttcgattcgt caaacagaac caggaaaata caccgatcgg attttcggta tggacaaccg
     6001 aagtttctat gtaccaaaag atttatagca aaaatttatt tttttcattc aaaacaaaaa
     6061 aagcgagaca taagtcttcg ctttttttgt tttcttttat tttgatgaat cagttgtttc
     6121 ttcagttgaa gattcttttg ttgaatcagt tgattttgtg tcagaactct ttgttgaagc
     6181 tgtagtttca cttgagtctt tcgtacttga tgaagttgtt gtgaatgcac tcaacacatt
     6241 ttcaaaggca tcatctttga tttttacatt tgcatctttt aattcttcac cgattacttt
     6301 agttgtgaat gcattgtcac ttagtttagt ttcagtagcg atatctttta gttgatcttt
     6361 gtatttatcc atgtcattgc ctttattttg gtttttcacc attttgacaa cgtagtattc
     6421 tgtcgcataa gaagtaggat ttgttgtagt aataacatcg gagatctcac cgtctttcaa
     6481 tttgaaagct gcttctttta cttctgcagg aatagttgtt gtcgttgagt cgaatttaac
     6541 tttaccgcca tcttcttttg tctctgtatc agttgatttg tctttggcta atttagagaa
     6601 atcatctcca tcatccgctg attttttcac atcttttgct tcatcttcac ttgataattt
     6661 gatgatttgt gcttctactt ctggatggaa agatttccaa gcagttttca aatcgtcatc
     6721 tgtaatatct acgtgagctt tcaagccagc ttcaaaagct aaattgttac gaatatattc
     6781 tttataagta tctttcgtat atccagcagc ttctaattgt gattcaaaag tatcacctaa
     6841 tgatttcgct tgtttgtcgt attctgcatc cacttgctta tcggttactt tatcgccata
     6901 tttgttattg aatactttat aaatgatcat ccgttgtact aaggattggt tcgatgattc
     6961 aagttttgct tcatcataga aatcagatac agtgatcgtt ccgcctttca tcgttgcgat
     7021 atctttatta gtatcgcctg aacaagctgc tagagttaaa acagccaacg cactagtagc
     7081 tgccaaaata attgattttt ttttcattaa aatttgcact cctgtaatta gttattattt
     7141 ttatatatta actataccat atcccatttc ctttaaaaac gttgtcgtgg tagtttcaaa
     7201 caaaattcaa aatttcatca cattcctagg aaatacgcaa acttttctca cttatatagt
     7261 taaatctgtt gagaaaataa tcattattat atttgttcag gatactctgg cagatcagct
     7321 tttagttttt ctacttgttc attgatttgt gtgatacgtg gttcggcttg gaatttaaaa
     7381 tcttcgatat cttttttgat tgcttcttgg aaagcaggaa tcacagattc agctgtttct
     7441 tttgtttcga tcaatgcgct tttaaaatga ttcaggctgt tatttaaatc atttgtcaga
     7501 gttgttgctt cttccaattc ttcgatcagt ttctttcttg tttcattccc gctcctgggc
     7561 gccaataaca gacctccgac agtgccagca gcagctccaa ataacagtcc tttgaaaaag
     7621 tttttaatca tcgcaggaca cctgctttac gattgtttct tgaatagctt ttagttcttc
     7681 cgatccataa ctatcttgat gattgacaaa gtgaatagaa aaatcatcct ctttgctgta
     7741 gcgagggact aaatggatat gtgaatgaaa aacagattga taggctagtt cacggttgtt
     7801 attcaagata ttaagacctt tcatttctgg aaaagctttt tccaatgctt gagcaatctt
     7861 aggcacacgc gaaaaaacct ctcctgcaag ttcgggatca tattcaaaaa tatctgctac
     7921 atgacgttta ggcacaacta atgtgtgtcc tttcgttgct tgagagatgt ctaaaaaagc
     7981 atagaccttg tcgtcttcat atattttata gcttgggatt tcttgattga tgattttgca
     8041 aaaaatacag ttttccatat aaaacatcct ttctataaag aataaatact tcagcttaac
     8101 tttaccataa gttaaagcta ttgtaaggaa aaaaacaaaa taccgtcatt ttcttcgtgg
     8161 tataataaat aaaaataaat ggcggaggaa aaagaatgag tttaattatc gatcatgtca
     8221 caggggggta cggacatctc cctgtgttaa aagatatcag tttcgatgta cagcctggag
     8281 agatagtagg gttgattgga ctgaacggag cagggaaaag tacgaccatc aagcacatta
     8341 tcggtctgct ggaagcttca aaaggaacga tagaaatcaa tcatctatcc ttaaagaaaa
     8401 atacggaaga atatcgcaaa caaattgggt atattccaga gactccttct ttatatgaag
     8461 aattgacatt gagagaacat atcgaagtca ctgcgatggc ctacgatata cctatggata
     8521 tagcaatgaa aagagcagaa aagttgttgg agacttttcg gttatctaat cgtcttgact
     8581 ggttcccagc taatttttca aagggaatga aacagaaagt gatgatctta tgcgcctttt
     8641 tgatcgagcc ttctttatat atcatcgacg aaccattttt aggcttggat ccactagcta
     8701 tcaatgcatt gcttgaattg atggtagaaa tgaaaaaaga aggagccggt attttgatgt
     8761 ctacgcatat tttagctact gcagaaaaat attgtgatcg ttttgtggtc ttacataacg
     8821 gagagatacg agcagacggt tctcttgaga tgttgagaaa agaatttcag ctgccggatt
     8881 cttctttgga cgaaatctat attgctttaa caaaggagca ggcacatgag tagttttttt
     8941 caaaaacgtt tagcaagaca tcaaaagaaa atgatgaaat atatgcgtta tgtattgaac
     9001 gatcattttg ttttagtttg tcttttcttg attggcggtg tgggattata ttattctaat
     9061 tggttgaaaa ccctgcagcc accgtttgca attggagggc tcataatctc tgtattttgg
     9121 atgctctgtt tattcgtggg taagatggct acttttgcag aggcagcaga tattgttttt
     9181 ttacttccta aagaaaaaga aatgcaaacc tatttggaac gagcttttcg ctattcttgc
     9241 atttttccgt tgatcttttt gatattggca tgcggattgg ccatgccatt agtggtggtt
     9301 agtacggagc aatccttttc gatgtttttc gtctatctga tcatattatg gagtttgaaa
     9361 ttcagtcatc tgcaaatcaa gagagcagaa ttgtttcgac tagatcaaca agttattcgt
     9421 tattggaaat taggctggtt tttttcttct ctgatctttt tgtttctatc attttattgg
     9481 ctaatatggg caggaatagc aggtgcatta gtacaggcaa gtctatatta ctggtttttg
     9541 tggaagcaac aagaaaaagg attggattgg gagaaaatgg tgcatgtaga agaacatagg
     9601 ttgcatcaga tctatcaatt tatcaattta ttcacagatg ttcctgaaat tacggcaaag
     9661 atcaaaagaa gacgctattt ggatcctata ttgactcata ttaaaagaga atcaaaaaac
     9721 acttatcttt acttgtttgc aagaagattg gcaagaggtt ctgattttag tggtctttat
     9781 ttccgattag taattatagg tgggttattg attgccggag tagtcgactt ttattttagt
     9841 ctgattatcg gtttcttatt tttatatttg atcggtttcc aactgttacc tttagcgaac
     9901 cagttccgct atatggttct cgttcagctt tatccaatcc ataagaatca gaagaagcag
     9961 gcgattcagt atctactcac atgggtatta attatcgcgt cggttatttt tggtttgatt
    10021 gctgcagtcg tattagatgg gcagcagcga tgggttccgc tagcggctta tttgattgtt
    10081 actatgctct ttgttaaact atatgtacca actcgtttga agaagatgtc agactgaaaa
    10141 caaagctgtg tagggtaaaa atgagaatga aaaatagagc gttgacagta agaatgctgt
    10201 tcgttctatt ttttttgaga aaagctggtt ttttgaatgg caattaaatg aaaaagaacg
    10261 tatatgtgct tgacgtgtag tgttcaggcg actagaataa taagtactgc aaaacaaaga
    10321 gtaagaggac gggagaatgc atgatggagt ttcagttaga ccaagagtgg cggttacgtc
    10381 ccatcaaagg cgacacagga aaaacataca taggcatgcg tgatgaggac aaagtcttta
    10441 ttaaacgaaa cacgactccg atgttggctg ctctttcaag ggaaggaatt acaccaaaac
    10501 ttgtttggac aaaacgaacg gggaatggcg atactttgac agcacaagag tggctggaag
    10561 gtcgcttgct gactcctaat gagattggtc aaagaaacga cgtgattgat gttttgtacc
    10621 atttacatca ttcaaattct cttaaaacca tgctgacccg tatcggtggt cgtgtcatga
    10681 cgccggagaa gatgctgcaa aaatatgaaa aagaattacc ggaagaactc cagaaaaatc
    10741 aatttctgca aattgtccat cgttttcttc gtgagcagat cccgtcaata gataaggaaa
    10801 acttatcagt agtacacgga gatgtaaacc atcgtaattg gttagtatgt aaaaattatt
    10861 tatatctagt cgattgggat tcaatcatgt ttgcagaccc tgcggtggat attgcaacga
    10921 ttttaggaaa ttatgtgcca ctgtctagct ggaatcagtg gctgatcagt tatggcatac
    10981 gcccgactga cgaaatgctt gaaaaatttc attggtatgc ggccatgaat ttgctccaag
    11041 aaatcaccag atattgcaaa cgtgaagatc atcgacggat gaacgaagaa attttacaat
    11101 taaaacgaat tttcagtggc tgaggtttgg cccaaactct gcggagattt ggccagacct
    11161 ttttaattag aggaaaggaa tggagataaa taatgcgtgt ccgaaatcgt ccgggagcag
    11221 cggaaatgtt agcggctcac ccgaattttg taattagtga tccaacattg tggaagggaa
    11281 aatggaatga attgtttgaa aacgatcatc caatccacat tgagattggt atggggaaag
    11341 gccagttcat cactggaatg gcaaaagccc atcctgaaat caattatatt ggagtagaaa
    11401 tgcaggtcag tgttgtttcg attgcactag acaagttgat cgaacaaccg cttcctaacc
    11461 tgaaacttct acatgtggat ggatcagcgt tgacagagta tttcgctgac agcgaagtag
    11521 atcaaatcta tctgaatttt tccgatccat ggccaaaaaa acgtcatgaa aaacggcgat
    11581 tgacatataa gacttttctt gctgtagatg aacagattct tcgtccaaat ggagaaatcc
    11641 atttcaaaac agataatcaa ggattgtttg agtattctct ggcaagtttt tctcaatatg
    11701 gcatgatcct caaacaggta tggttagatt tacatcaaag tcaatttgaa ggcaatatta
    11761 tgacggagta tgaagaaaaa ttttcttcaa aaggtcaacg tatttatcga gtagaagctc
    11821 aatttcaaga taagaccaaa aagaaaagtc tgtgaaacta actttctgct ttcataaaaa
    11881 cattaatatc cataaataaa tcccagaaaa tgaacattac agatgtaatg tattttctgg
    11941 gatttatttg atttaaaatg tatttatttc atcggttttt ccacaaggtg gaatcaattg
    12001 gaagcgagaa aatgaaaata atcctttgtc ggtgatcttc aattcgggta tgacagataa
    12061 tgttaagaaa gaaagtgtca aaaaaggatc aaaggtttta gctgctccta gttgatgtgc
    12121 tttagttatt aattcattca actgttcata gacgatcgaa aaaggttcct gtgtcatgat
    12181 tccgcctata ggaagagaaa cacaagctat ttcctgctgt ttgttgacaa cggtcatccc
    12241 gccacctttt tgaatcaaaa gattagcagc atacagcata tcctcgtcgt ttgtcccaac
    12301 gacaactaga ttatgcgagt catgggcaat cgttgtcgct atcgctcctt gtttcagctg
    12361 aaatcctttc actatgccta atccaatgtt tcccgtttga tggtgacgct cgatcaccgc
    12421 aatctttaat tggtcttttt cgattgaagg aatgaatcgt tcgccttgtc ggtccacttt
    12481 ttctaccaaa tctttagtca ctagactatt cgggataatt tcgatcacat gggcttgtga
    12541 agaagaaaga agcagctcta acgaatgtgg atttaatggt tgagcgttta gttttggcag
    12601 acaacacgta tatgaatttg aatgatcagc aaataaatgg tcttggagtt gcccagattg
    12661 aacaactaat gttcccttat agaaaacttc atgaattggc agcttggtaa gatcgtctgt
    12721 taagaagaaa tcagcttgat acccaggggc aatcgcacct agtgttttca aatcaaaaca
    12781 ttctgctgca ttcaatgttg ccatctgaat ggcttgtaat ggatcgattc caagtgcaat
    12841 agcttgacgg acgatatgat cgattgatcc ttcttttact aaatcattga tcagtttatc
    12901 atcggtgaca aagaagcatc ttcttgaatt ttcaggtgtg atcgccggta gtagcgatgc
    12961 cagatctttt gcaacggtcc cttctcgaac cattaaatac ataccaagat ccaaacgttc
    13021 tttagcttct tttgcagttg tagcttcatg atccgtccga atacctgctg caggataaac
    13081 atttaactct tccatgccga taccagctgc atgcccgtca atttttttct tatgctggtg
    13141 catcaattgg atcttttcta ggatgtctgt ttcattattt gctactgctt gatagttcat
    13201 gacttcagct aagcctaaaa cttcgggttt ttgtaaaaaa ggggctaatt tttttgcatt
    13261 taaatttgca ccattggtct caaaaggggt tacaggtaca caagaaggta gcataaaaaa
    13321 gatattcatg agtgtttgtc ttgcatcttc tatcataaac tcaagacctg tactcccagc
    13381 aatgtttcct atttcatggg gatctgtcac tacagtagta actccatgaa gcaaggatac
    13441 ttttgaaaat tcgcttggtg ataaaagtga gctttcgata tgcacatggc catcgatgaa
    13501 tcctggcact agatattgcc cattagcatt atgtacgctt tttccttcgt attcaccgat
    13561 tcctacgatc attccttcgc agattgctac atctttttcc atgatttctt tcgtaaagac
    13621 gttgacgatc ttagcatgct tgatcactag gtcagcaggc ttttttttac ttgctacttc
    13681 gatatatttc cttaatgttg tttccaatgg agtgcctccc aagataaatt tttataagta
    13741 taacataaat tcggtttttg ccaatatttt tttgtaaatt cattttattt gaaacaaaaa
    13801 gatcaaaaag acgtatgttt tttgtggagg ggagaataat ggaaataaaa cgattatcgg
    13861 atcaaatgat aagtgaaagt tttgccaaac atgcccaaga cgtatatata ttgccagctt
    13921 cagaagtatt ttacatctat tttcgctatg gaaaaaaacg aacattctgg aagaaagtct
    13981 cttcggcaga tggacagcaa ctgatctcaa gatttaagta tttgggacag atggatatcg
    14041 gagaaaaaag aaaagctcag ttaggtgcat ttacttattc tttagaaaaa caggaagttc
    14101 gtctgcggtt gtcttctgta ggagattacc ttcaacgaga gagtttagtg attcggctat
    14161 tatacggtac ttttgaaaca ccccatcgtt gctttagttc tcgagatttt gcaactattt
    14221 tacagaaaac cgtcagacgt ggattatacc ttttcagcgg accagtcgga tcaggcaaga
    14281 cttctctaat gtatcggttg gctttggaag aaaaattgca agtgattact atcgaagatc
    14341 ctgtagagat agaagaaccg ttttttctgc agctacagat caatccgaaa atagcacaga
    14401 cttatgatac acttttgaga ctcgcattac ggcatcgtcc agacttgcta atcattggcg
    14461 agataagaga tgaatcaact gcacatgctg cggtaagagc agcactaaca gggcaccggg
    14521 tttttgcaac cgtacatgca cgcaatcttg aaggtacatt ggtcagaatc catgaactga
    14581 caaagaaaaa agaagagcta tctgaatgtt tagctggtgt catttatcaa gaacttttgg
    14641 aagatgccaa tcaccatcct tctgttttat ggagttatca atttatggag gaagaatcag
    14701 taaaaaaatc atgggaggaa agttatgaag aagctcagga aaatatttgg cagtcagaac
    14761 tattctaaag gattaacaag aagccagcaa aaccagtttg cacaaaccat gggagaactg
    14821 cttttatctg gttttagtct gcaagaatcc ctttatttat tactcacgat ccgttcgttt
    14881 ccacctgtta ttttggcaaa tatccaacat cagctaaagg aagggcaagc gtttagtgag
    14941 gtactttcgc aaataggata tggagcagag caactggtac agatcgagtt agctgaacgt
    15001 catgggaatc tgccgacaac tttgttggaa attgccaaac agtttcggct tgttgaagga
    15061 tttcgcaatg atttgaaaaa gctgcttgca tatccgatgc tattgctcac ctttttgcta
    15121 ggtatcttgt ttagtatgcg gttgttcgtt ttgcctcagc ttattggttc aggaatggtc
    15181 atgcaagagc attggggtat tcagctgata caagtctttc attggtatgt tctactattc
    15241 atcctagcgg ttactctatt ggctttacta gtaaggtatc gtctgatgaa gttgtctcct
    15301 gttgatcaga gtgaatggat aagcagacaa ttattgattg gaccgatata ttccttgtat
    15361 caatcggctt atttgtcact agagattgga aagctttttc atgaaggttt cgaattaagg
    15421 caaatcatag tctgcttaaa ggaaacgaaa caagaaagtc tgattcagca tcttgcctct
    15481 aagttgattc aaggattgga ggcaggtatg tcattggctg agcagttcca gaattatcgt
    15541 tttctatcag aagaattcag taaaattgtt ttacagggag aggcaaaggg aagtttaggt
    15601 aaagaactga ttttttacag cgaatggaca agaaaacagc ttttcaaacg tcttcatcac
    15661 tggatgcatt ttattcagcc aatcattttt ttgggcgtag cggctttgat tttattagtt
    15721 tatgccgctg ttttattacc tgtttacgga agcatagagg aggttttgcc atgaaaaaga
    15781 gaaaaatatc ttattatagc ggttttacat tgatcgaaat gctaatcgtc ttactgatca
    15841 tcagtgtatt agtgttgttg ttcgtcccta atttgtcgcg ttataggaat catgtagacc
    15901 aagaaagtcg tgaagcgatc atccagttgg tagacacaca aaaagagctc tatgcgcttc
    15961 aaaataatgg aagagttcct actgtggagg aattgttgaa tgaaggctat atcaaacgag
    16021 aacatgcaga aatctatcag cggccttgaa ggtaaggttt tagcatcagg atatacgttg
    16081 tttgaatcac tcttactgat tttattactt acaacgatcc tttcttttcc agttctcgca
    16141 ttttctgcat ggaagaatga attggcagtc tatcaatttt tcggccaatt tgaaaaacga
    16201 atctattcta cgcaaaaaat cgctattgtg aatcagatcc aaacaggctt ttattggaat
    16261 agtgaagaga atcaaatcat ttttcatgtt ccgaatccag ataaaataaa ttgggaagta
    16321 ttagatattc ctgatgagat taccttgaaa cgccatgctt ctattacttt tgctgctaga
    16381 accgggaatg aaagttcact aaaagcttat caattttatt gggaagaaaa agagcggacg
    16441 attacgtatc aattccagat agggagtggt cgctatacaa aaagaattga ataaaacagg
    16501 atttgttttg ttggagtccc ttacagcgct tatgatcgct tctgtctgtg tctacttagt
    16561 gagctttggc cagctgtttt tgcttcaggc agaaaagaaa agtggggagg aactgctatt
    16621 tattcgaatg atttatgaag atatcaagac gcagcggatg tatcacaaaa gaccagagcg
    16681 aagggaagaa atgatcccat acgatatcca aattgtcgat aataatggag taatcgaaaa
    16741 agtgtttgtc aatatggaga aagaagcgat cagtattgaa aaaacaagta attccagcat
    16801 ttacgctgat tgaatgtcta attggactga tgatattgag cagtctattg ctcacattca
    16861 atcttttgat tcagcaaacc atccaagtaa gtgaattttt gcagcgtaaa gatcaaaaag
    16921 agtggctaat atttttagca caattagaag aagaactcaa gagcagtcac aatgtctctg
    16981 tgaaaaatca gcaactgcac tacacgatcg aggataaaca gtatatcatc gaaagatatc
    17041 aggcaatgat acgtaaaaga agaacttccg gcggacatca acctatgtta acaagtatca
    17101 ctgagcttca attattggaa aaagaacaca caatttcttt ttatgtacac tttgaaaatg
    17161 gagaagatgg atatgccata tggacaaaaa acgatcagta gaaaatgtta taagggctct
    17221 attttatttt ctgtcttgct tttatttctt ttatgtacgt ttttattgat ggcagcatta
    17281 gaaagctacc gtttgtcaat ggatcttaat atacgaatca aaaattatta tatagcaaaa
    17341 ataatgaagc agatggcttt atcggaacag tctacgcttg ccgaaagttc ggaaggagtt
    17401 ttttactaca caactggatc tgtaacatat cagtgggtac aacagtccct attcttagag
    17461 gtggaagtct caccgtttat ctttcggttc acagaagaag taaaaaatga tacagatact
    17521 agtacagaat gatggaatgt tcgtgaaaac gccctctttt ctgttataac actgttttta
    17581 ataaaaaaat gtttgccgtt tcacaaataa aaaacagtga aaacgattga aattttgtca
    17641 attgttcgat agaattaaac aggtgaaaaa attgttataa tatatctaga aatgaggtgc
    17701 accatgttac cagaaaaaat ggaacaaggg tttaatcaaa acctggaagc tattcagctt
    17761 ttacagaatg cattgggaac ttccttttta gaagcctatg tcgaaaatgc agaaaattta
    17821 atagatgatt atcaagtacg tgtggtggat ggagtgccga caaaagagac aacgcaacgg
    17881 atcacagctc tttatgaaga gttgaagaaa ctttctttcg agccagaaga atggcgcaga
    17941 ctgtctcaat tacttttatt gaagggcagc cagacagaac atttgcaggc gaaccatcaa
    18001 ctcactcctg atagtatcgg ttttttattt gtttttctta ttgaacaact atacacaaat
    18061 aaaaaagcac cggttaagat tctggatatt gcggcaggaa tgggcaactt gttattaaca
    18121 gttcttttaa acctatcgaa tgcggggtac caaacagaag gaattggtgt ggatattgat
    18181 gatacattgt tggctgtcgc agcttctaca agtgatttga cacaagcaaa tgttcaatac
    18241 tttcatcaag acgggttgca ggaattatta atagatcctg tcgattttgc aatcagcgat
    18301 ttgccaatcg gttattatcc gaatgacgaa aaagcaaaag agtttttaac aagtaccgaa
    18361 gaaggccata gttatgcaca tcatttattg ctagagcaat caatgaaata tgtgaagccg
    18421 gatggttttg gtttgttttt gatgccgagt ggatttttag aaacggatca aagtgaagaa
    18481 atcaaaaaat ggtttaaaga agaaggttat cttcaaggaa tgattcaatt gccagatgaa
    18541 ttgttccgta ataaacaatc gcaaaaaagt attttgatct tacagaaaaa aggaccacaa
    18601 gcacaacaag taaaagaagt cttgcttgtg aaacttgctt cattaaaaga accagagaag
    18661 gtgacggaat ttttcaacga gtttaaaaac tggaaatcat ctgctctgta atcattcaac
    18721 gagaaggaga gtcatatgtc taaaacaatt gcaatcaatg ctgggagttc aagtttgaaa
    18781 tggcaattat atcaaatgcc agaagaaacc gtagttgcga aagggatcgt cgaacgtatc
    18841 ggtttacaag attctatttt taccatcaag tacggcgata atcaaaaatt cgaacaaatc
    18901 atggatatcg aaaaccacga agtagcagta aaaatgctgt tagatcagtt gattgaactg
    18961 caaattttaa gctcttatga tgaaatcacc ggagtagggc accgtgttgt tcacggagga
    19021 gaaacttatg gggattcagt agtgatcgat gagaaagtaa tggaccgcat tgctgaatta
    19081 gcagaatttg ctcctttgca taatcctgca aacttaatgg ggatcaaagc gtttaaaaag
    19141 attttacctg acattttgag tgtggctgta ttcgatacat ctttccactc aacaatgcca
    19201 aagcaaaatt atttatatag cttaccggtg tcttattatg aagatttcaa agtaagaaaa
    19261 tatggcttcc atggcacaag tcatcgttat gtgtcggaac gtgcggcaaa attattgaac
    19321 aaaccgatcg aagaactaaa aatcatcact tgccacttag gaaatggtgt atcgatcaca
    19381 gcagtcgatg gaggtaaatc catggatact tctatgggat tcacaccatt agctggggtg
    19441 acaatgggta cacgttcagg tgatatcgat ccagctgttc tcccatattt aatggaaaaa
    19501 ttagagattg atattgatga aatgatcaat gttttgaaca agaaatctgg tctgctaggc
    19561 ctaacaggat tgtctagtga tatgcgtgat cttgaaaaga actatgaaaa agaacatatt
    19621 cgtttagctt atgacatttt cgtggaccgc atccgaaaat acatcggcgg ttatgtgaca
    19681 acgatgaatg gtgttgatgc aattgtcttt actgctggta tcggcgagaa tgacggacat
    19741 gtacgtagtg aaatcatcaa aggtatgtct tggtttggtt gtgaaatcga tccagcgttg
    19801 aatgaattgc gtggtgaaga agtagatatc tctacaaaag aatcaaaagt aagagtatta
    19861 gtgatcccaa cagatgagga actaatgatt gcccgtgatg tggagcgtct gcgcggctaa
    19921 gccaaagcat tcgttaggat aaaagatgaa acgcatgcaa gagggaattc ttgcatgtgt
    19981 tttttttgcg gagaagaccg caaaatggta atttaacttc aatttccctt cttttttaca
    20041 ggatcttctt agggaatttt gcttggaaaa gtggtcaaaa gggaaaagac tggcatttta
    20101 tttattgatt ttcaggaaaa tcccaatatt ttattttcat tcatctttat aagggacatt
    20161 acatatgtaa aaagaaaaaa catttttaga tcagcctcgt gggctgtcga ctttattctt
    20221 cgctttatgt ttagctagaa aaccgatccg aaacattcat tgatcgaata ataaaaactt
    20281 gattttccac ctgtactttg cctgaatcgt gaaagcacag gtatgaaaat caagtttttt
    20341 gttgcgaaaa aacgtcctag tcaatcagtt ggccgagcgg ctggcggctg atctgctcct
    20401 gatcaataac gagtgcttct tggaagtgtt gctgcaaaaa gtagcggtca tggctgactg
    20461 ctaggagcgt tccttgatag tcttgcagca tttcttcaat ttcttctcga gcatagctgt
    20521 cgaggtgatt ggttggttca tccaaaatca ggacgttgat tttttctaaa aagagtttca
    20581 gcaacgccaa acggattttt tcaccaccgg ataaatcttt gatccgttta cccacatcgc
    20641 tttgataaaa gccacttttg gctaattgcc gtcgagcgac ttcttcgttt ccagtcaatg
    20701 tgatggcgta agccagcaaa cggctatcgg gatcggcaaa ctgcagctgc tgcggcaaat
    20761 aaccgatttg caagctacta cctaatttga tggtgccacc gtaaggcttt tcctcaccga
    20821 gtaataattt gagtaaggtt gattttcctg caccattgtc gccgatgatg gctaaatgat
    20881 ctccacgata gactgcaaaa ctgctgtctt tgaatagtac ttgatcagcg tactgctggt
    20941 gcaagttttg aatgatgaaa acttctttgc cagattttcc accattagac aagctttgca
    21001 gccgattttt cgggggaggt ggcggtttga cgagtgtcag tttcgccagg cggcgttcca
    21061 gttctttggc ttttttgaag aaggactcat tgtctccttc atgtgcccac tgacgaaaac
    21121 gacgaatcat gactttcaac cgctggactt ctttttgctg cagttcgtaa ttttttgtca
    21181 gctcagccaa gcgagcttct ttcaagactc gaaaacggct gtagtttccg ggatactcaa
    21241 tcaatgcacc ttcttccagt tcccatgttt ttgtggtgac ttgatcgaga aattgccgat
    21301 catgagaaac gaccagaacc gatttttttg tattctgtaa atagttttcc aaccagcgaa
    21361 tactgttaag atctaaatga ttggtcggtt catcgagtaa aagtaaatcg gcatcggctg
    21421 ctaaaatttt ggctaaagcg actttaactc gttcgcctcc gctaagttct gttaaccgtg
    21481 cttcctgacg gtgtggaatc cctaagcctt gcaagatcgc tgcaatccgg tcttctaata
    21541 cgtaaccgcc tagttcttca aagcgatgct gcagctcacc gtaacgggcc agacacttct
    21601 ctaagtttgt atctggttct gtcatcaatg cttcgtagcg actcatttgc tgtttgaggt
    21661 cctgtatctc agcggcagaa tggagcaagt agtggttgac ggtcgcttcg gaaactgtaa
    21721 gttcttgttc gaccgtcccg atctttaagc cttttttgcg actgataaca ccgctgtcga
    21781 caccttctct gccggtcaag atttgtaaaa aggttgattt gccagttccg ttgataccga
    21841 ctaaggcaat tttttcacca gcttcaattt gacaggataa atcctcaaat aatggattcc
    21901 cgccgattgt ttttttaata gaaattgctt gtatgatcat gatctctctc cttgagacca
    21961 ctcaaaaagg ccatggttat ccatggcctg tgttatttaa actcagatta tcatgagaaa
    22021 aataccttga gatggccatt gattttacgt gaattcaact gttttggaca aacttgtccc
    22081 gtcaacagca actgtaaaac caattgcatg gctaaatgcg atatggtatc gttggtaatt
    22141 ttcattactt gttattttag tcatgcgcat catagtattt tccctccttc gtgtagttgt
    22201 cttttccagt gtacctgaaa gtgtcagaaa gttcaactag cgctgacatt tacttaatat
    22261 ttatcgatgt gtaattctgt cagagagctt tttttatctg actgagggta atgtctcgtg
    22321 gaccacatca ttgtattctg agctaaacca gcgttcaggc gaaatcatat ggtgtttgat
    22381 aaaaaacttt gtctacatga cttccttttg ccatttgctt aataccgttg cattaaacag
    22441 ctcttctctt tggaaatctc accaacatcg cgttcaggag tatcgttgtg gttgcaatgg
    22501 caagcggtag cctttcatca attcttgaat gatttaccga atgtagcact tgccttctcg
    22561 cggtacccga acccgaaata aaattttagt ttggcgataa aatcggtatc cgccatcgat
    22621 acttcatatt ttttcgtatc agattcatcg gaaacttcca cattgacgaa ccaataagct
    22681 ttggcatgct taggtcgttt taaaccacac cagggcgact aatcgccctg gtgttcactt
    22741 ttttttattt tacttgaact cattgactgt tttcccttta ttttaaacat tataaagacc
    22801 gatgagtgct tgccgcgtct tttgttttta tcacggtttt ttgtgcaaaa aaacctgccg
    22861 atagttggca ggtcgtctac gtgttaagct ttggtgtggt ctttagctgg caattggttt
    22921 tctaaatcag ttcggacaac aagcacatcg caagctgcgt ttctaataac atattcagaa
    22981 acagaaccaa taaataaacg ttctacagca ttcaatcctg tggctcctaa cataatcaaa
    23041 tctacttgat tatcttctgg gatttctctg gcaataattt gttttggtga gccatattct
    23101 acgacagagg ttacattatt caagccagct ttcttcgcat tgctttcata atctgccaat
    23161 gtttgctttg ccatttcagt agcttgttct gcaagcattc catcaaagga tgaaacggtt
    23221 tggaaagaac gtgtgtcaat gacatgtgct aatagaagtt ctccgttatt tcggatagct
    23281 acattgaccg cctttttaaa agctaattca gcttcatcag aaccatctac ggcaaccatg
    23341 atttttttgt attgttgtaa cataagcatt cactcctcaa gtcaatcgta ttgtactctt
    23401 ttattgtaga acactttagg gaaaaagaga attatttact gctttggttc agcaattttt
    23461 tttgcaaatc ggatactgaa ggtggcagaa acaataatta cgatcagtaa aaagaccaag
    23521 gcaataaagc gttgattgaa aaaagctgtt aaaataccca ttacgcctaa aacagcatat
    23581 gcagctccgt aaaattgtag gaatccttga tttttatcat ttttgttgat caatacaaaa
    23641 aagatatcct gtttattaaa taaataatag ctaacgaata ataatactaa agtggcgata
    23701 atcattaaaa tcctaatcat gaacaaatcc tccaaaataa gcaacaaata gaaaaagcaa
    23761 tttgccaaga gcaaattgct ttattcttta aaagatggta atctccgtta atgccgttgt
    23821 ttgtcctgta gtattgatcc cgcgaattca agcaggtggg tcccatggtc gtagcttcga
    23881 actaccaaat gataaggcat gttaccagca gtgacacaag acgggtccca agatatcaat
    23941 aaaaatgttc ggtcaacaca caaagaggac aattcgaaca tcacagattt cccacctcta
    24001 tagtagcata tgaatttaaa aagagcaatg agagagctcg cagaaacaga accgttttct
    24061 tgtctagttt tttggaggat gctttttcca ctcttttata cggtaatact gttgacccag
    24121 agcctgttca tatttccctg tatcttttgg ctgataatac tgagcattct tcagtttatc
    24181 tggtaaatat tgctgattga cccaagcctg atcaaaatga tgaggatact gataacccac
    24241 gccacggttg agtgatttcg cacctttgta atggctatct ctcaaatgat caggaacatc
    24301 tccagctttg ccctctcgaa tatcagcaag tgctgcatct aatgccatgt aggcagaatt
    24361 ggattttggc gacaagcaaa gatcgacgac gacgtctgct agtgggatac gtgcctcagg
    24421 caatcccaat ttttcagcag ctaatacagc gttcactgta cgagccgcag cagcaggatt
    24481 acctaaacct atatcttcat aaccgataac catcagacgc cggcaaatac ttgccagatc
    24541 acctgcctca actaaacggg cgagataatg aagtgcagca tccacatcac ttccacgaat
    24601 cgatttttga aaagccgaaa taacgtcata atgagcatct ccatttttgt catgggtaag
    24661 agcttttctt tggacacatt cttcaatgat cgacagtgta agatgaatcc ttccttcttt
    24721 atctgaagga gtcgatagtg tagccagttc cagcccattc aatgcactcc gcaaatcgcc
    24781 gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct aactggattg cttgttgtcc
    24841 aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc aactggatgt cttgttctgt
    24901 taatggtttg acttcaaaga tctgtgtacg gctgcggatc gctggattga tggtaatata
    24961 aggattctca gtagtcgctc cgatcaagat gattcggcca ctttctaaat gaggaagcag
    25021 aaaatcctgt tttgttttat ctaatcggtg tacttcatcc aataagagga tcactgtacc
    25081 actcattttt gcttcttccg ctacaacttg tagatccttt tttgaatctg tagcggcgtt
    25141 tagcatgcga aatgcataat tagtagagcc ggcgatcgca cttgcgatac tcgtttttcc
    25201 agtaccagga ggtccatata gaatcatgga agagagcatg cgagcatcga ccatgcggcg
    25261 tatgattttt ccttccccga ctagatgctg ctgaccaacg acttcatcga tcgttcgagg
    25321 tctcatgcga taagccaatg gctgttgcat aggattttcc tccttcttgt ttttacgtcc
    25381 cttaattata tcatatttag caagatgcct agaaagaaca gccttctgtt gttttgtttt
    25441 catagcttat ccaactaata ttgtgtatga taaagtaggt gagaaaaatg acggataaac
    25501 aaaagataga tgaatttttt tggaataaaa cgacgccaga agtggcacgg gatttattgg
    25561 ggatgtattt ggagcatgac acaccagaag gaagattagc tggatatatt gtagacgcgg
    25621 aagcatacct aggtccagaa gacgaggccg cccatagcta tggtttaaga agaacaccgc
    25681 gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct ttacacgatg catactcatc
    25741 ggattttgaa catcattact cagccagaag ggattcctca aggagtaatg atacgtgcaa
    25801 tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg tggcggaaaa actggtccag
    25861 atatcagcaa cggtccggga aaattagtgg aagcattagc aattcctcaa gaactttatg
    25921 gtcagtctat agcagatagt tcgttacgct tagtttttga gaaaaaaaag acaccaaaaa
    25981 agatcaatgc tttgcctaga atcggtatcc caaacaaagg tgtatggaca gaaaagccgt
    26041 tgcgttttgt tgtgtcggga aatccttata tctctttaca aagaaaaaac caaatcgaga
    26101 aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc tattaacata tttagatgaa
    26161 ataattgaaa aacaagtgac agactatgat gtagctttag attgggatac aaaaaatcat
    26221 acgatcgaga tcgttgttcg cttgtttgca gaaaataaag cacaatttga gattgatgat
    26281 gcagaaggga tcgtttcgca agaaccaatt atcgaatttg aagacggcgt acttcttttt
    26341 aatcctcaga aatctgtttt tgatgaacgc aattatctag cagtaatccc ttatgaagga
    26401 aaaaaagggt tggcaaaatc agtagctgat tctttagtgg aatatctaaa tgaagtatta
    26461 gctcaagggc aaagcgattt gcttgatttt ttggatgaag acgatgacga agctgtattc
    26521 gagcttcact gggataatca gcaactagca gaattagtag aagtaaaaca gcaaaatgaa
    26581 gcagatactt atttaccgta cccaagttat taaggagtga cagatatgaa atggaatgaa
    26641 gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg ctaatatttt gatggaggca
    26701 ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg aacattttca gtcggatcaa
    26761 tatggtgaga ttttagataa aaatgatttt acatcgctga aagaaggcgc actagtcatg
    26821 gcttattttc cggaaacgac tttcttaccg gaaatcttgc cttttatcaa agatcgtatc
    26881 acacaattac ctgagtttgg tctagctatt ggaaaaaata aagtaacagt tagtgaagta
    26941 gaggaaagca actgggcaac agcctggaag aaatactatc atccggtgcg tattacgcga
    27001 atgctgacgg ttgcgccaag ctgggaaaac taccagacag aagatcctct agaaaaaatt
    27061 atcacactag atcctggtat ggcatttggc acaggcactc atcccacgac ttccttgaca
    27121 ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat tgctagatgt gggaactggg
    27181 tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa aagatgtcta tgcctttgat
    27241 ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg atatgaatga aatagccaaa
    27301 gatgtccatg tatcagctaa cgatcttttg acaggaatcg agattgaaag tgatgtaatc
    27361 gttgcgaaca ttttagcaga tatcatctta ttgatgatcc cagatgcttg gcgtttactg
    27421 aagcaaacag gcacgctgat cgtttcagga atcattgaag agaagaagca aatggttctg
    27481 gatgccatgt tagaacaagg gtttatcgtg gatcaaattt tccagcaaaa agactggtat
    27541 gcaattattt tgaaaaaacc agaggtggaa tagatgcagc ggtatttttt aaaagaatca
    27601 tatgaatcaa aagactattt tgttttagaa ggggaaaact atcaccacat tgtacgggtt
    27661 atgaggatgg aacccgagga tgaagttttt ttggcattca atgatcaaat agccattgtt
    27721 gcagaaatca cggagattac tgaagaggct gtttatctga aagaaattaa aaaagaagaa
    27781 aatcaaaagg aactgccaag ttcgattacc atagcgagtg gctatcctaa aggggaaaag
    27841 ctggattgga tcgttcagaa atccactgaa ttaggagcgc aagcttttct tggtttcccg
    27901 gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta tgaaacgaca acaacggctt
    27961 gaaaagattg ctcaagaggc agcggaacaa tcccatcggc agatcgtgcc atcagtagag
    28021 ttattggaaa cgaaacaaga tttatttgag cggctagatg attatgacgt ggtcttggtt
    28081 gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg cagccaaaat tcttagtgaa
    28141 acaaaaccag atagtcgaat cttagctgtc tttggaccag aaggtggatt atcccgcgat
    28201 gaagtcgatt attttgtaga acacaaaggg atactttgtg gattaggacc aagaatcctt
    28261 cgtacagaaa cagcacctct ttatttatta agtgctgcta gctatcaatg ggaattaatg
    28321 aatccttctt tcagctaacg ttcttcatta ataaaagagt atcgacagaa agaattgaaa
    28381 aaatagtcaa caatttgtat aatagaaaca attatttata caatggatac gaaaaatcgt
    28441 ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa cgtataactg cacggatttt
    28501 gggacaatta tcaaacagat atttgtcccg attcccgttt taccgaaaaa agaaaggggg
    28561 atcgattcct gtgacaaaag aagaaatttt aacgggaccc ggtgtcataa agctcgttag
    28621 tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa gcatgcgaat acgcgactgc
    28681 tgcacatgac ggacagttta gaaaatcagg cgaaccctac attattcatc ctatccaagt
    28741 cgcaggtata ttagcagatt taaaaatgga tccccataca gtggctacag gcttcttaca
    28801 tgatgttgtt gaagatacag aaatcacttt agaagatctg agagaagaat ttggcgatga
    28861 cgttgctatg ttagtggacg gcgtaaccaa attagggaaa ataaaatata aatcccacga
    28921 agagcagcta gcagaaaacc accgaaagat gctgctagca atggctcaag atttacgagt
    28981 tatcatggtc aaattagctg atagactaca taacatgcgt acgttgaagc acctgcgaga
    29041 agataagcaa agaaggatcg ctcaggaaac tttagaaatc tatgcgcctc ttgcacatcg
    29101 tctagggatc agccggatca aatgggaatt agaagataca gcacttcgtt atctaaatcc
    29161 aaaacagtat taccgtatcg tccacttgat gcagacgaag agagaagaac gtgaaaaata
    29221 cgtaagtggt actgttgaag atattcgaat agcgacagaa gagctgggga tttttgcaga
    29281 aatctatgga cggccaaaac acatttattc aatctatcgt aaaatgaaag atcagaaaaa
    29341 gcagttcaac gaaatttatg acttgctagc tattcgagtg atcgtagatt caatcaagga
    29401 ttgttatgct gtattaggag caatccacac aaaatggaaa ccaatgcccg gcagatttaa
    29461 ggattatatt gccatgccaa aagctaatat gtaccaatct ttgcatacta ccgtgatcgg
    29521 tccggcagga aatccggtag aaattcaaat ccgaacacag gaaatgcatg aaatcgctga
    29581 attcggggtt gctgcacact gggcctataa agaaggaaaa aatgaaaaag tagaaccaga
    29641 tggtatgacg aaacaattaa gctggttcca tgagatactc gaacttcaag acgaaagcta
    29701 tgatgcttct gaatttatgg aaggcgtaaa aggagatatc tttagtgata aagtctacgt
    29761 cttcacacca aaaggagacg ttactgagtt accaaaagga tccggaccat tggactttgc
    29821 atacagtatc catacagata tcggtaacaa aaccactggt gcaaaagtaa atggcaaaat
    29881 ggtgcagctt gattacaaat tgaaaaacgg agatatcatt gagatcatga cttctccaaa
    29941 ttcatttggc ccaagtcgcg actggttgaa attagttgct actagcaaag caagaaataa
    30001 gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat gtgatcaaag gccacgaatc
    30061 cgtggtcaaa tgtattacag atctaggatt tacgcctaaa gatattttga cgaagaacaa
    30121 actgcaagaa gcactcgatc gttttaatta tcaaacagaa gatgatctct atgcagctgt
    30181 agggtatgga gaagttagcc ccttgacgat ggccaatcgt ctgactgaaa aagaacgtaa
    30241 agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa atcatgaatc agccgaaaaa
    30301 agaacctgac aaaatgaaag tacgtcatga aggtggcgtt gtcattcaag gggtagaaaa
    30361 cttactaatt cgtatcagtc gctgctgtaa tccgattcct ggtgatgata tcgttggtta
    30421 tatcactaaa ggcagaggga tatccattca tcgtcgagat tgtccgaatg ttcagcctga
    30481 caaaccaaat gtagcagaac gtttgattga agtcgaatgg gaagatacat cgaatacacg
    30541 aaaagagtat gatgcagatt tggaaattta cggctataat cgttcaggct tattgaatga
    30601 tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac agcgttgaag cacggacgaa
    30661 taaagataaa atggcgacga tccatttgac ggttggtatc cagaatttat cccacctaaa
    30721 gagtatcgtg gataaaatca aagcagtacc tgatgtctac agtgtacgcc ggacgaatgg
    30781 ataggaggac agatatgaga gcagttgttc aacgggtaag taaagctgct gtgtcagtcg
    30841 atcagcaaga aattggaaaa attggctacg gcctacttgt tttattaggc attcatgaaa
    30901 ccgatacgca aaaagatgtg gactatctgg taaaaaaaat tgcacaaatg cgtatttttg
    30961 aagatgaaca aggaaaaatg aatattagtg tagaagatgt gggaggacaa attttaagta
    31021 tctctcaatt cacactgttt gcggatacga aaaaaggaaa tcgaccaagc tttgtggaag
    31081 ccgcacgacc agaaacagct attcctttat acgaatcatt caatgaagga atcagaagtc
    31141 gcggcatcac agtcgctacc ggacaattcg gagcagatat gtcggtttct ctcgtcaatg
    31201 atggacctgt aacgatcatt attgattcta aaaacaaata aagtaatctg gttttcacta
    31261 gtaaaaacta tgaaacacgt cgcttaagtt agactgataa actctaactt aagtggcttt
    31321 tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa aaaagttaag cttaaattat
    31381 acaagttatt ttttatagga ggttgctgaa gatgaaagaa gtaccagtag tagatatcaa
    31441 aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac atgttggagg agttacgcca
    31501 tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat aaagaagcta tcacgatcta
    31561 cggatcatca gcagcaaaag tattttatga tccaaggaat ttcaaacgaa aaggtgctat
    31621 gccaaaatta gtattgaaga cactttttgg tcaaggtgga gtacagactt tggatggagc
    31681 tgctcatcac catcggaaaa atatttttat ggatctaatg acaccggaaa gaatggaaga
    31741 ttatcatcgt atcttagata agaatctgac acaagcactt gaagcacagc atggtcaatt
    31801 tgaactattt gatttaagta agatggtttt ctttacatct atttgcgagt gggcaggtat
    31861 caatttatca gcgatttcta aagatgaggt cgaaaaatta gcagaatatc aaatctctat
    31921 gatcagcgga acgtttactt ctccgattga tcacataaaa ggtgtcgaaa atcgcaaaaa
    31981 atcagaaaaa tgggcgcaag gcttgattga agaagctcga caaaatcctg tggctggaaa
    32041 agaaaatgtg gcattatatg cttttgccaa tgccacagat ttagatggtc agcttttacc
    32101 tctggaagtg gcagctgttg aattattgaa catcatccgt ccaacggttg ctctaactgt
    32161 gtgggcggca ttgatgggac atgcactttt cagtagacca gatttgtatc agcaattaaa
    32221 aaatgacttt tcgacgttac aagatccgtt tattcaagaa atgagacgct attatccttt
    32281 cttcccaatg ctacctgcga tttcgctgaa agaagtagaa gtagatggtt atcgtatacc
    32341 agaaggcagt tgggttattt tagatttata tggaacagac catgatgaac gaacagttga
    32401 agcgccagat tcctttatga tcaaacggta tgtagggaaa gcaaaagata tttcctataa
    32461 agaagaatat gagatgattg cacaaggcgg gggaaatttt cgacaaatgc atcgctgtgc
    32521 aggagaatgg atcactcttc atagtctacg tgtgttttcc gatcagctag taaataagtt
    32581 tgagttctct gtacctgaac aagattggac gattccattc aatcagtttc cgacttatcc
    32641 gaatagccga gcgctacttt acaagaatta gattagcata aacttatcga aaaaaaggaa
    32701 gatcaaacag tgctgatctt ccttttttca tattactata aattgataag gtcaattgat
    32761 caaaatccac gaaatgcttc atcaatggtt tgataatctt cattggataa atgcaaatct
    32821 aaagcttgga cattgtcaga tacttgctct ggacgcttag ctccaggtat aactacagac
    32881 actcgtggat tcttcatgta ccaagctaaa acaagttgcg caggagtggc ttggtatctt
    32941 ttagcaatgg ggcgaagtac atcgacagcg gttaaagttt ctctgaaacg ttctccttgg
    33001 aaatctggat ttctttttcg aggatctcct tcaccaaatt gtttttcctc accaagttca
    33061 tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg gaacaaaact gattttgttt
    33121 ttttcaagat aagggaataa ttctttttca gcctgccgat ggatcaaact gtatttatct
    33181 tctactacat cgacatatcc atctgcattc gcttctttca attgttctaa cgtgaagttt
    33241 gacacaccga ctgctcggat ttttcctgct tcttttaatt cgtgcaatgt agcaactgat
    33301 tcattttttg gtgtgctttc atccggaaag tggatataaa aaatgtcgat gtaatcagtt
    33361 tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa actcaggaga attatcaaat
    33421 gttcgccctt tatttggtac atgagcagct tttgtagcga tgacgacacg tgagcgatca
    33481 tattcttttt ccttcagtac ctcgccaatc agttcttcgg aacggccgtt tccgtaagca
    33541 taagcggtat cgatcaaatt gatcccgcta ttcaaagctg tacgaactac ttgttttcct
    33601 gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa cggcatttgc tcctaatcca
    33661 agctgttctg cgtagacttg tgaatgtccg attcttattt ctgccatagc tgattcctcc
    33721 tttttgtcta gtctaaaaca aaataccaaa aagctgaact aaaacgtttt tgtgagggga
    33781 aatagtcaga tatctcttga ctttgagact ggtttttagt aggttataag taatatcgaa
    33841 tgtcaatgaa agagcgagta agtttttgaa actttacaag agaggatcat cgaggctgaa
    33901 aatgatctaa agtttcatta gcctaaagac actctggaga tggacgagtg aaaagccgtc
    33961 ccgcgtacga gcgttattcg taagaggaaa gagaaatctt tcaaaattag gtggtaccgt
    34021 gtatgaattt cattcaccct tgttcatgat gaacaagggt gattttttta tttacatagc
    34081 taagaaaggg agagaaaact atgagtttcc aaagaccaaa aggaacaaat gatattttgc
    34141 caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc acgtttatta ttcagagatt
    34201 atcagtataa tgaaatgcgt acaccgattt ttgaacacta tgaagtgata tcccgaagtg
    34261 taggggatac aacggatatc gtttcaaaag agatgtatga tttttatgac aaaggggaac
    34321 gccatgtgac gcttcgacca gaaggtacag caccaatcgt tcgttctttt gttgagcata
    34381 aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt ttatatggga ccaatgtttc
    34441 gctacgaacg ccctcaaaaa ggacggttac gtcaattcca tcaaatcgga gtagaagcat
    34501 ttggatcaga aaaccctgca actgatgtgg agagcatggt gatggcactt gatttcttca
    34561 aacagttggg gatcacgcaa atccggctag tcatcaattc attaggtgat aaagaaacgc
    34621 gtcaagccta tcgtcaagca ttgatcgact atcttttacc ttttgaatca gagctgagtg
    34681 aagactctaa acgccgcctt catgaaaatc cattacgcgt attggacagt aaagacaaac
    34741 gagatcaaaa atttgtggca gaagctccat ctatcttaga ttatctaagc gagcctgctc
    34801 aaaaacattt tgataccgtt gtttccatgc tggatgcatt ggctgttcct tatgaaatcg
    34861 acagtaatat ggttcgcgga ctagattatt acacacatac gatttttgaa atcatgagtg
    34921 acgcaccaaa aatgggggca caagcaacga tttgcgcagg cggacgttat gacaatctag
    34981 tggaagaact aggtggtcct tcaacacctg gcttcggatt tgcaatgggt atcgagcgat
    35041 tactcttgac tatggaggca gaggaagtag ttatccctgc atttaatgag ttggatgctt
    35101 acgtggtcgc actcggagac gaaacaaata tcgaagcatt gaaagtggtc caagcgattc
    35161 gaaactttgg tttttcagct gaccgcgact tcatgaaccg taaagcaaaa gcgcaattta
    35221 agacagcaga taaagcgaat gccaaattag tattggtgat cggtgaagat gagcttgcta
    35281 gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga aaaaagtttt ccattagaag
    35341 agatatatga acattttgat gaagtctatg atgaaatgac tttattcgag taggaggaaa
    35401 aaaataaatg gcacaaagaa cgatctattg cggattagtt tccgaacaac aattagaaca
    35461 aacagttaca ttaaaaggat gggtacaaaa aagacgagac cttgggggag taatctttat
    35521 cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt aatcctgaaa tcaacaaaga
    35581 agcatgggaa atcgcggata aatgtcgcag tgaatacgtg atcgaagtga ctggggaagt
    35641 tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc actggtgaat tcgaagtgat
    35701 tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg ccatttcaaa ttgaagatga
    35761 tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat ttggatctgc gtcgtccaaa
    35821 aatgactaaa aacatcaaat taagaaatgc gacaacaaaa gcgattcgtc attatttaga
    35881 tgataatgac tttctcgata tcgaaacacc ttacctaggg aaatctacac cagaaggcgc
    35941 aagagactac ctagttcctt cacgtgtcca tgctggacat ttctatgcgt tgccgcaatc
    36001 tcctcaattg tttaaacaat tgctgatgaa tgctggattc gaccgttatt atcaaatcgt
    36061 tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag cctgaattta cgcaagtaga
    36121 tattgaaaca acgttcttaa cagcagaaga aatccaaact tacacagagg gattgattgc
    36181 gaaggtaatg aaagaagttc gcggtatcga agtcacattg ccatttcctc gtatgaccta
    36241 tgatgaagca atggctcgct acggcagcga caaaccggat acacgttttg atatggaatt
    36301 gatcgatcta agcgatacag tcaaagaagt agaattcaaa gtcttccaaa tggctcttga
    36361 aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct gcagatcgct actcacgaaa
    36421 agacatggat cagttaggtc aatatgtagg acaattcgga gctaaggggc ttgcatggct
    36481 gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa ttcatgggag aagcaacaga
    36541 agcaatcatt aaagcaacag atgcaaaacc aggtgacttg ctgatgtttg gcgcagataa
    36601 aagtgaaatc gtcgctgctg cattaggtgc tatccggaca cgtttaggaa aagaattagg
    36661 actaattgat gaatcgaaat tcaatttctt atgggtaact gattggccac aatttgaatt
    36721 ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca ttcacgatgc caaaagaaga
    36781 agatatcccg ttattggctg aagaccctgc aaaagtctat gcagaagctt atgatatcgt
    36841 cttgaacggt tatgaactag gcggaggttc tatccgtatt catactcgtg agcttcaaga
    36901 aaaagtacta gaaactctag gctttacaaa agaacaaatg gaagaacaat ttggtttctt
    36961 gttaacagct ttagattacg gattccctcc acatggcggt attgcattag gattggaccg
    37021 ttttgcaatg ttattagccg gagaagataa tattcgtgaa gtcattgcct ttcctaaaaa
    37081 cggaaaagca gcagatgtga tgagtgatgc accaagtatg gtttcgcctt tacaattgtt
    37141 cgaattgaat attgatgtaa cgaatataga agaatagccg ataaaaaggt aaaaaggatt
    37201 tgtcgaatac gcttcttttg acaaccttga tttaattgaa aaacacttta taatgaagcc
    37261 ataacatata aagcggggct tttccatgaa aaaaacattc gaaaattggg cttatcatga
    37321 ttacacgacc aagctttcgg tatctatcgt ttatgcgttg atcgcatcag tcgcattgaa
    37381 tttcttttat cagccaggac atatttattc aagtggtatt actggtctag ctcagatttt
    37441 gacgactttg tcaaaatcac tgataggatt cgaagtaccg gtatcaacaa ctttgtattt
    37501 actgaatata cctttgtttg tattggcgtg gttaaaaatc gggaagaagt ttacaatttt
    37561 cacttttatc acagtcactt tgacctccct ttgtatccat attttaccgg aaacagtcct
    37621 atctaccgat ccaatcatct gtgcgttgtt tgggggggct gtgatgggac taggtattgg
    37681 gctagcgttg agaaacggcc tatcatcggg cggtctagat atcgtgagta tcacgattcg
    37741 taaaaaaaca ggaaagtcga ttggttctat ttcgatctac ttcaatgcat tgatcgtttt
    37801 tgttgccgga attctatttg gctggcaata tatgttttac agtacgttgt ctattttcgt
    37861 cagtggaaaa gtaactgatg tcgtttatac taaacaaaag aagatgcagg taatgatcgt
    37921 aacgaaacat ccagaaaaag tagtaaatga aatccaaaac tgtatgcgta gagggatcac
    37981 gatcatcaat gaagcagaag gtgcttataa gcatgataag cagacagtat tgatgacgat
    38041 cgttacacga tacgaactgc ctctacttag agaagcaatg aaagcttctg atccacaagc
    38101 ttttgtaagt attgcagaaa acgtccagat acttggtcgt ttctatgagg atggcattta
    38161 gatcgagttt gacaaaaatt catgaaatac tgctgtagac agacgttcgt ttgtctgcag
    38221 tttttttcta ccatagtaga atcagcaaag aaaaactgct tttttatttg aaattttctt
    38281 aaagttgaaa aaaagcatgt ttattttttt aaatttatgg gatgatagaa aaaaatagta
    38341 gagaaaggac acgattctac ttgctatttt cacttaatac ccgtatatta cgaatgaaaa
    38401 aagtttatga aaagttggaa tattgaatgg aaaaacaaaa aaggttaaaa aacagccgct
    38461 acatcactgg gtttgatggg ctccgttcat tagctgtggt gggtgttatc ttctatcacc
    38521 ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc gatttttttc gtcgtatctg
    38581 gttatttgat cacggatctg ctgcggcagg aatgggaaca aaatggaaaa atcaatattt
    38641 ggcaatttta tgtcagaaga atgaagagac tctatccggc acttgttttt atgcttgtga
    38701 cagcttcggc ttatatcact ttgtttcagc gagggctgtt aaacaatctt cgaggagttg
    38761 tggtcagcag cttactgtat gtcaacaact ggtggcaaat caataacggt ttatcttatt
    38821 tcgatcgatt tgctaatgag tcaccgttta cgcatatctg gtctttagca gtagaaggtc
    38881 aaaactattt ggtttggccg atcttgtttg tattgttgat ggtatttgtt cgtaaaagaa
    38941 aatggatctt ttatactgtg ttaggtacat ccttcctctc cgccatcttg atgatgatct
    39001 tgtatacacc aggaggcgat ccaacccgtg tttattacgg taccgacact cgtctgtttt
    39061 ctatatggtt gggaagtgcg cttgcgtttg tttggccaag tacgcgttta aagaaaaaca
    39121 tcccgaaaca agcgaaaaat attctgaata tcgctggagg aatctcgctt ttggcactga
    39181 ttctttgctt cttcttttta gatgatcatt acaatttcat ctattacgga ggaatgctcc
    39241 tagtgagtct gttatgcgta gtgctagttg cagtgacagc acaccccgga gcaagtttga
    39301 atcgatggct gacaaatcca gtatttactt ggattggcaa acgaagctat ggcatttatc
    39361 tgtatcagtt tccagttatg attttttatg aagcaaaggt gactgatatt gccgatcatg
    39421 ttttgttgca tactttagtg gaaatcacat tgattctaat catcagtgaa ctttcttatc
    39481 gatatgtgga gcgtccgcta gcacgttttg attatggtcg tacattccaa gtgatgaaag
    39541 gatggttcag caaaccgatc atcagtaaga gcaaaccatg ggttgttcca ggcacattac
    39601 ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa aaacgctgta acagccgatc
    39661 aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt agcagaagaa accagaaaca
    39721 attcagaaaa caccactagt tcggttgacc aagcgatcct tgataagtac gagttgtcca
    39781 aggcacaagg gaataaggca caaaaattag aactaacggc atttggtgat tcagttatgt
    39841 tggatgctgc aacggatcta caagaacttt atccaaaagt agtggtagat ggagatgtcg
    39901 gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt aaaggaacaa gatttgttga
    39961 aagacactgt tctgatcgga ctaggaacga atggctcatt ctcggaagct cagtttgata
    40021 gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt caatgtccga gtaccgacga
    40081 agcgctggca aaatgacgtg aatgcgatgc ttgaacagat gacgaaaaaa tacgacaata
    40141 tgacgctgat tgattggtac agctacagta atgaccatag tgactggttt tatgatgatc
    40201 aagtccatcc aaatcctgaa gggatgacac attacattca cttagtcagc gaagccttgc
    40261 taggaggaag ttcaagtacg aacagcagtg agacaaacgt gacagaatga tagctacaaa
    40321 aaacttcagg caaaaacatt tgtttttgcc tgaagttttt ttatatttac cgtgttttta
    40381 acattcatat agattcagga agcaaaacgg ttgagtgtgt ctgtaaataa cgttcttttt
    40441 gttttcgggt gagttttttg aatgcagctt ctgcttttgt tgcttcgctt cttgaagaaa
    40501 atacttctgc atggatcatc ttaacaggtc ttcttttttg aagacgagta tatttagcac
    40561 cagtaccatt gttatgttcc tctaatcgtc tagaaagatc ggttgtatat ccaccataaa
    40621 atgtctgatc tttacagaat aatacgtaaa aataatgttc atttgccata gagcatctca
    40681 cgaatctctg cagtgtattc tccatccata ttgtaagtga tcaatggtgg agcgactttg
    40741 aatccgtcta atttgccttc tttgattcct tcaactaaca aaatatttgc ctcttttcca
    40801 ggttttgggt aaacaaattg gatcctttta ggtgcaattc gatagtcttc catgacctga
    40861 atgatttcta agaaccgatc aggacgatgg accatagtaa agcgtccatt tgtttttagt
    40921 agcttacttg aaatatcgat cacttcttct aacgtcgtat gaatctcatg acgggcgatt
    40981 gccaggtgtt tattagggtt cgttttattt gttggcagcc ctttgaaata aggtggatta
    41041 cagacgacta agtcacaaga attatgtcga acgacagata agctgtcttt cagatcgatg
    41101 gtgtggacag tcatttgttc atttaaatgg ttaagcgcga tactacgtct tgccatgtct
    41161 gctagacgag gttgaagttc gatagcgtcg atagccgcct gggttcgttt acttaaaaaa
    41221 agtccaactg cgccatttcc tgcacaaagg tcaacaatct tccctttttt aggtacacgg
    41281 gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa acactttgga actttggata
    41341 atcttgatat cttctgcgta taattggtcg attcgttcgt cttcgtgaag catggattcc
    41401 ctccaaaatg aaaaaaatag tcgtctgtat tatactattt ttattaaaaa gaaccaaggt
    41461 ggcttgtcaa acctctattt tttatgcata ctatgaccat aagaagggaa aggaagagac
    41521 tatgttttat cgatttatgc gcggggtagt tcgagtacta ttagccttag ttaatggaaa
    41581 tgcccgctat gagaataaag aagttttacc gcaagatgag aattacatac tagttgctcc
    41641 ccatcgtact tggtgggacc cattatatct ggcggtagct gcagcaccca aggaattcag
    41701 ctttatggca aaagaagaac tgttcaaaaa tccagttctg cgatttatat taactcgatc
    41761 caatgctttt ccagttaaaa gagaaaatcc aggaccaagc gtcattaaaa cacctgtaaa
    41821 gattttgaaa tcgacaaatt tgagtttgat catgtttccc agtgggacaa gacatgctac
    41881 agaacttaaa ggcggaatgg cattgatttc cagaatgtct aaagcgaaaa tcgtgccagc
    41941 tgtatatcaa ggtccgttga cacttgggga tctgttcaag cgtcagagag tgacagtgcg
    42001 tttcggcgaa ccaatcgact tatcagatat caagaaaacg gataaagaag gtatggcgga
    42061 gatcgaacga cgaacacaag cagcttttga caaattggac aaagaagtga accctgattt
    42121 caaatatgaa gtcaaaaaaa aataaaatga taagtttatg cgtgggcggt cttttgaatc
    42181 tactgtcctg ttcagttcag actttttcaa tgaaaacgag ggagagacgt caagtctgcc
    42241 ctctttttgc gttccaatat gtaaaataga agacaggtgt catgtaatgg atcaaagtgt
    42301 tataataatt agaaggacat tttttgaggt gattgaatgc gttttttaca tacagccgat
    42361 tggcatatcg gaaaaaaact acaaggttat gacttgattg aagaccagcg tcatgtacta
    42421 tcttctatac tagatatcgc gaaagaagaa aaagtagagg caatcgtgat tgccggagac
    42481 ttatatgata ggagtgtccc agctgttgat gccgtagaat tatacaatga attgatggtc
    42541 gattggaatt taaaggaaaa gatcccgatt tttgctatat caggaaatca cgatagcagt
    42601 acgagacttt cagcgggtac gccatggttc agttattcga actatttttt gcgaacacag
    42661 ctagcagaag catttgaacc agtcgagttt aaaaacgtac aatttttctt tcttccttac
    42721 tttgaaccaa ttgcagcgag attatatttt gaagatgaca atatccgaac gatcaagcaa
    42781 gcggtagcca aggtgataga taaaatgcag gagctatttg attcagaaaa aaaacaagta
    42841 ttgattagcc atttttttgt aatgggaagt gtaaaatctg attcggaaac gaaaatcgaa
    42901 gtaggcgggt tggattcgat ccctggtgaa ctacttacac cgtttgatta tgtagcacta
    42961 ggacatttgc ataatcaagc tgctttgaga caggataatg ctcgctatag cggttcccca
    43021 ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg tatggatcgt tgacctgaca
    43081 acaggcctgg agttgaattt tagggagatc gaacccctgc atgatattga agaaataaca
    43141 gcgagtttca aagaattgct ggatcctgcc ttttatcagt ccgtcaatcg ggagaattac
    43201 atctatatct atctcactga tagaacgatc atcccgaata tgatgaacca gcttcggcaa
    43261 atatatcctc gtattttggg gattgaacgg gcaaacggtc gagcagaaca aacaaagcgc
    43321 aaacgaaaag tactagaaat gaaagatcct caaaaattag cagaagcatt ttttagtgaa
    43381 atgacggaag agcagctaac tgcaaaccaa gaaaaatggc tcggagaaac gttgcaagaa
    43441 atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat tacaacttaa aaattttggt
    43501 ccttttatca atgagaccgt agatttttca cgtttgacag aagcaccgct ttttttgatc
    43561 agcgggaaga caggagctgg caaaacgacg atttttgatg ggatcacctt cgcacttttt
    43621 ggtgaaactt ccggaagact tcgctctgga aaagagatgc gttctttgtt tgctacacca
    43681 gacgaagaaa caagcgtgac tttttctttt gaacatcagc agatgagcta tgaaatcgaa
    43741 cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg gaacaaaaaa acaggcagcc
    43801 aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa taaaacaaat cacaaaacgt
    43861 acagaagcag atcagttgat cagagaactg atgaatctcg atgcgaaaca gttttctcaa
    43921 atcgttttac taccccaagg agaattccgt aattttttgg tttcatcaag cagtgataaa
    43981 gagactgttt tacgtcattt attcggtact caatttttcc aacagttcaa tgagcgatta
    44041 aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt tagagcaaga gctgcatctt
    44101 ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag cagcagggtt tgaagccgtt
    44161 ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac aaatcacgga agaaaaagaa
    44221 gctctaacgg aacttcagga aaaacaaaag caactagaaa attcctacta tcgttttgaa
    44281 agcttgaaaa aaagttatga agaaaaaacg atattaataa acaagcaaaa ggaattagct
    44341 gctcaaaaag acgaaataga ggaaaagaaa cgatggattg ggtattatga atttacgcaa
    44401 cgattagtcg aaccaatcca gcgcctacaa gaaataaaag ttgaaaaaat agaactgtca
    44461 caaaaaagta gtgagcaaac aaagaaacta gcgatgacta cgcagaatta tgaagagtgg
    44521 caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa tcaaagaagc tgctcaaaaa
    44581 ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct tacgtagtaa aaaacaagac
    44641 gcagaacaat taacaatttc attggataaa gaagaattga atgccgagaa gctgtatcaa
    44701 aatctcgcta tctatcagga aagaactcga attcttcaag aaaatttact ggatcaggaa
    44761 aaactgcaag aagatcgtct tgcttttgga cagttaaaat atctagatga aaagcagcaa
    44821 gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg cacagataag agaagaactc
    44881 aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat tgaaagaaca gacagaacaa
    44941 gcagaacagc tgaaaagcca atgggcaaag cagcaaattg ctcgtcttca gctttttttg
    45001 cgtccagggg agccatgtct tgtttgtgga tcaacggaac accccataca acaattaaaa
    45061 cacgaacagc caaataagga agatatctta aaagtagaac aacaactggc agaagctgag
    45121 tctgctgtag aaagaactca gcaaatcatt gccaaaagtg aagcacatga aaataatctt
    45181 caacaacaat tatcagattt agaacaaaaa atcatggcaa cgaatataca attggctgaa
    45241 caagaagaac acttgcaaca ggctttgaaa aaggcatttc ctgagataac agaacaaaaa
    45301 acaaaaacaa ctcttgtagt aatcgaacaa tctttagata gacgaaaaag caagatggcc
    45361 aatgcaagaa tagagctgga agaaataaga caacaagcag aaaagctcga acaacaatta
    45421 gctgtctaca aacagcagca gacagaaaaa aataatcagc tccagcaact gcaaggagaa
    45481 atcaaaagtt tagaacaaca attgcaagat acggatagta actgtttaga agaggagata
    45541 cgacgacttg aaaaacagtt agaagaagac tcgaatcaac tagctgttga gcagaaagca
    45601 ggagtgcaat tgttgcaaga tcttcatacg cttcggacac agcttgatct tcaacatgaa
    45661 caaatagaaa aaatagatga aaaaatagaa aaggaacaag aaagaatagc taaaaagtta
    45721 gaaagccagt cgtatttttc aacaattgaa gaaatctgtg catttgcaca acagcaagca
    45781 caatataaag aaaaccaacg tacagttact tcttatcatg aagagcaatt agtgacagct
    45841 gaccggctgc gtcaattaga aaatatccag ctaactgaga cattcccaga tacagaagag
    45901 atcagccaac agttagaaga aataagacgt atgatcgaaa taaagcaaga aaagatatac
    45961 ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg atgaatgtct ttcgatttat
    46021 cacacaagcc agacgaaatt agatgagctt acgcaattac agcagctttc gcaaacattc
    46081 aacggagaaa atcctaagaa gactagtttg gaacggtatg tgcttcaggt ttatcttcaa
    46141 gaagtgttac aagtagcaaa tgatcatctc caacgtttaa cgaaaaatcg ttatcaattt
    46201 gagctagcgg ataccattgg cagttatcga gggaaaaccg gattagaaat caatatctat
    46261 gatgatcagg caggtatgtc tcgaagtgcg cataccctgt ctggcggtga gagttttatt
    46321 gcagcacttt ctttagcatt agctcttgcc gatgtgatcc agacacaagc tggtggtgta
    46381 gcaatcgatg cattgtttat tgatgaagga tttgggtcat tagatgaaga agctttggaa
    46441 atggcaatgg aagctttaga aacgatcgaa agtgaaggaa gaatgatcgg catcattagt
    46501 catgtaagag aattgaaaga acgagtatta caacaaataa gaattgatac agaaggtagc
    46561 ggacaaagca aaatacgcta tcaacttgga taaaaaggga gattggtaac tatgaaatgg
    46621 agtattccag aaaaagtcat tgcacgtgga cgtcaatact tgcaagaagg ccgagtgtta
    46681 tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag aagtcttggg cagtgaattg
    46741 tatatggttg atttggatgg tacagcaaaa gagatagatt actgccaatg tccctattgg
    46801 gaggaacata aatattgtaa gcatacagtt gctgtggaac tttatttacg gtcgaaaaat
    46861 ctatctcgta tcatgaaaaa agaccagcca atgctagaac cggtaaaagc aacaagtgag
    46921 ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac aaaacaatcc tcaagcggtg
    46981 caacctttga ttattgaata ccaagtagaa acgattgaga ctaatcagta tcacccagag
    47041 ctttctattt tggcacttta cttgcgtatc ggttcattgg catcaccgaa aaaaacatat
    47101 atcgtaaaaa acatctatga ttttttacaa gcttacacag aaaaagaaac ttacactgtt
    47161 aataaacagt accagtttcg tctagataaa cgggcgtttc aaaaagaaga tcaaaaaata
    47221 ctcacgcatc ttgcaggaag tgctcagacg catcaattat taggtacgaa cggcttacaa
    47281 gtcaaaggaa aactagataa aagatatctg cttctcccag tagaacaagc ccgcttcctg
    47341 ttagagcaga tgaatcagac tacacgtttg catctgcaga taaatgaaca aaacgatcaa
    47401 ggaatacaat tttccgatca aaacaaacct ttatcattca aagtggaaaa atttggagaa
    47461 gattatttgc tgcaagcatt gaatgatttt gattttttct ttatgcatta tcagtgggga
    47521 gttatccaag ggacatttta ttctctgacg aaatatcaac aaacggttta tcaaacttgg
    47581 aaacaattga ttcgccgatt ggagaaacca gaagtagtgt tcaagaaaaa agaattaccg
    47641 tctttgttta aagagattat ccctctgctg tcagagatcg gcgacgtagc tattgcccct
    47701 gcagtagaag aggaagtagc agatatcccc gtcgaattta ttttcttttt ccgaaaagca
    47761 aaaggaaaaa tccaagctcg agtcgatttc gtctatggcg aagtcatcta ttcgactgac
    47821 ccgaagcatg aggtctcgac agaacaggct tttcaggtgt tacgtgacac agtagaagag
    47881 aaacgagtac ttgatctatt cagaatgtat cgctaccaga aaaatgaaac tggttatgaa
    47941 cgtccattgc caagcgggga agaactatat gcctttttcc gcacagaact atctgtcttc
    48001 cgtcagtttg gtgaagtcag acttggaaga aaattgcgag agttgtattt agatgcgcat
    48061 aaacatcaac cgaaaatcga agtaaccgag accggttcat ggctcgatat tcgattcgat
    48121 gtgacaggga tagaggagca agaaatcgat catgtcctgc aaagcttgct gagaaatgat
    48181 gctttttata cattagaaaa tggtcaggtt ctttcttttg attcagaaga atttcaacaa
    48241 acaagccaag tgttgcaaca attacgggaa tcgatacgta cagaagaagg aacgatccat
    48301 gtacctaaaa atcaaggatt gatcattcaa aatcaattgg aaaaaagcaa tgctactttt
    48361 agtgaatcgt tccaaacgat ggtacaagat ctcattcatc cagaaagata ccaggcacag
    48421 cttcccaaag gattgaatgc aacgatgaga gattatcaaa agcaaggttt tcgatggttg
    48481 aagatgttag gtcattatca gtttggcggt attttagccg atgaaatggg acttgggaaa
    48541 acactacaga caattgcctt tttactttcg gaaaaagaag aacggaaaag cttctctgct
    48601 ttgatcgtag cgcctgcgag cttaatttac aactggcagg cagaggtgag aaaattcgcc
    48661 ccttctttaa gtatccaagt aatcaatggg aataaaaaag aacgtgaaga attactggct
    48721 aaagacacag atatccgagt gacttcttat gccagcttaa gacaggatct agctaattat
    48781 cagtcgcaaa aaattgatta tttgattctt gatgaggcac aaatggtcaa aaatagcagt
    48841 acgaaaactg cacaagcatt gagagaatta gctgtacctc agcggtttgc actgagtggg
    48901 acaccaatcg agaataattt agaagaattg tggtcattgt ttgcaacgat tatgccagga
    48961 tttttcccga ctaaaacaaa attcagggaa cttgccgcag aagaaattgc gcaaatgatt
    49021 cgaccgttta ttttacggcg agataaacaa actgtgttga aagacttgcc ggaaaaaaca
    49081 gagatgaatc tatattctgc acttacagaa gaacaaaaaa ctgtttattt agcttacttg
    49141 cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag cattcaagaa aaaccggatc
    49201 gggatacttg ctggtctgac aagactgcga caaatttgct gcgatcctcg tctgtttatt
    49261 gaagattacc aaggcggttc cggcaagctc gaacaagtaa aagatctact ggtagctgcc
    49321 aaagagaaca aacgaagagt tttgctgttt tctcaattta cagggatgct gactatcttg
    49381 caagaagagt tagccgagct agggatctcg acattttatt taagaggcag tacgaaacca
    49441 caagatcggt taagtatggt agacgcattt aatgctggcg aaaaagatgt ttttctgatt
    49501 tccttaaagg ctgggggaac tggattgaat ctcacgggag cagatacggt catcttgtat
    49561 gacctttggt ggaatcctgc cattgaagaa caagctgctg gacgtgcaca tcggatcgga
    49621 caaaaaaatg tggtagaagt gtggcgtatg atctctgaag gaacgattga ggaacgcatg
    49681 gattcactcc agcaagaaaa acgcgaactg ttccaaaaag tgatccaagg taatgaagaa
    49741 cagttgacga aactgactga agaagacatc cgcatgattt taagtgtagg agaaatcgag
    49801 gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa agcctttcta aagaagcgat
    49861 tgtttatttg atttaaaaaa tagcaaggag gctgggattc cattttatcc cagcttcctt
    49921 gctatcattt ttttatttct atttttaaag gttatacaaa gattgtcttc attgtctcta
    49981 acagctgatc aaaagaagaa atctcgatat cgcaaattgg agtctgttca attttgcgtt
    50041 ctcgatggtt gaaccaaagc gcttgccagt tagcactttt tgcacctaga acatcattgt
    50101 cgtaattatc gccaacgtat aaggtctctt ctggaagcat atgaaattct ttctcagcta
    50161 gctgaaaaat ctctttttct ggtttttgga aacctgttgc ttgagaaatg agcatattgc
    50221 gggaaggaat ccaattgttc aattgaagtt gcttcagttt ttttgtctga tggtcagtcg
    50281 gcccatttgt gataattcct aatgggacat tcttttcctt caagaaatcg agggtttttt
    50341 tgacctcctc atgcaagcag atattatcaa gttcttcctc gtaaattttt tgaaataaga
    50401 ggccatcttc ttccgtaata tgagggtagt ctaaatcttt tagtgattgg ctgatccgat
    50461 gtgctcgcat atattctagt gtccaatctc ctgccattac ttttgggaag ttttcatcac
    50521 tatgatggcg gaaccggata tatagtggat gcatatctgc atctgaaaca agcggaacga
    50581 ctcttttgac cgcgtttcga aatggctgct gctgatcata catcgtatca tcaacatcaa
    50641 acacaactga tttaatcaaa attttacacg cctttcggtt ttcattgatt ttttcacaaa
    50701 tccttgacta ttgtaacaga aaaaattgtc taaaaaagct gataaataaa ggtttttctt
    50761 actatatttc tatttccttg acagaataag gtttttatat ttcagttttt ttcactaaaa
    50821 atcgtgaaac acgtcaataa gtttgatgaa caaattaatc attatggtag aagtcgattg
    50881 atttatttta atgcaatctc tgtggaattt tcgaaagatt tattttttta atacagtctc
    50941 aatataacta ttcaaacata aaataacgtt atttgattta tttgtttata gtaaattt
//
LOCUS       DACDCJ010000010        50792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDCJ010000010 DACDCJ010000000
VERSION     DACDCJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.010"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2258)
                     /locus_tag="IV038_000589"
     CDS             complement(<1..2258)
                     /locus_tag="IV038_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8340832.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDIL"
     gene            complement(2686..3759)
                     /locus_tag="IV038_000590"
     CDS             complement(2686..3759)
                     /locus_tag="IV038_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8340833.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(3756..4583)
                     /locus_tag="IV038_000591"
     CDS             complement(3756..4583)
                     /locus_tag="IV038_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340834.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(4584..5390)
                     /locus_tag="IV038_000592"
     CDS             complement(4584..5390)
                     /locus_tag="IV038_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8340835.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGERKKGGRS"
     gene            complement(5402..6487)
                     /locus_tag="IV038_000593"
     CDS             complement(5402..6487)
                     /locus_tag="IV038_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8340836.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(6504..7046)
                     /locus_tag="IV038_000594"
     CDS             complement(6504..7046)
                     /locus_tag="IV038_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8340837.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            7304..8293
                     /locus_tag="IV038_000595"
     CDS             7304..8293
                     /locus_tag="IV038_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8340838.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            8290..9027
                     /locus_tag="IV038_000596"
     CDS             8290..9027
                     /locus_tag="IV038_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP8340839.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            9024..9575
                     /gene="coaC"
                     /locus_tag="IV038_000597"
     CDS             9024..9575
                     /gene="coaC"
                     /locus_tag="IV038_000597"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP8340840.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            9591..10223
                     /locus_tag="IV038_000598"
     CDS             9591..10223
                     /locus_tag="IV038_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8340841.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            10308..10955
                     /locus_tag="IV038_000599"
     CDS             10308..10955
                     /locus_tag="IV038_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8340842.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            10960..11943
                     /locus_tag="IV038_000600"
     CDS             10960..11943
                     /locus_tag="IV038_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP8340843.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(12346..13860)
                     /locus_tag="IV038_000601"
     CDS             complement(12346..13860)
                     /locus_tag="IV038_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP8340844.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            13998..14804
                     /locus_tag="IV038_000602"
     CDS             13998..14804
                     /locus_tag="IV038_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340845.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(14979..15548)
                     /locus_tag="IV038_000603"
     CDS             complement(14979..15548)
                     /locus_tag="IV038_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340846.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(15631..17691)
                     /locus_tag="IV038_000604"
     CDS             complement(15631..17691)
                     /locus_tag="IV038_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340847.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(17968..19446)
                     /locus_tag="IV038_000605"
     CDS             complement(17968..19446)
                     /locus_tag="IV038_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8340848.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(19744..20679)
                     /gene="murB"
                     /locus_tag="IV038_000606"
     CDS             complement(19744..20679)
                     /gene="murB"
                     /locus_tag="IV038_000606"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP8340849.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(20752..21672)
                     /locus_tag="IV038_000607"
     CDS             complement(20752..21672)
                     /locus_tag="IV038_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8340850.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            21785..22537
                     /gene="xth"
                     /locus_tag="IV038_000608"
     CDS             21785..22537
                     /gene="xth"
                     /locus_tag="IV038_000608"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP8340851.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            23401..23934
                     /locus_tag="IV038_000609"
     CDS             23401..23934
                     /locus_tag="IV038_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP8340852.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(24424..24948)
                     /locus_tag="IV038_000610"
     CDS             complement(24424..24948)
                     /locus_tag="IV038_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340853.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(24941..25414)
                     /gene="tsaE"
                     /locus_tag="IV038_000611"
     CDS             complement(24941..25414)
                     /gene="tsaE"
                     /locus_tag="IV038_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP8340854.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(25537..26517)
                     /gene="pta"
                     /locus_tag="IV038_000612"
     CDS             complement(25537..26517)
                     /gene="pta"
                     /locus_tag="IV038_000612"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP8340855.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(26670..27350)
                     /locus_tag="IV038_000613"
     CDS             complement(26670..27350)
                     /locus_tag="IV038_000613"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8340856.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            27476..28345
                     /locus_tag="IV038_000614"
     CDS             27476..28345
                     /locus_tag="IV038_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8340857.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            28371..28886
                     /locus_tag="IV038_000615"
     CDS             28371..28886
                     /locus_tag="IV038_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340858.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(28933..29496)
                     /locus_tag="IV038_000616"
     CDS             complement(28933..29496)
                     /locus_tag="IV038_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340859.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(29551..29730)
                     /locus_tag="IV038_000617"
     CDS             complement(29551..29730)
                     /locus_tag="IV038_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340860.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(29807..30316)
                     /locus_tag="IV038_000618"
     CDS             complement(29807..30316)
                     /locus_tag="IV038_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340861.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(30412..30792)
                     /locus_tag="IV038_000619"
     CDS             complement(30412..30792)
                     /locus_tag="IV038_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8340862.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            31122..31871
                     /locus_tag="IV038_000620"
     CDS             31122..31871
                     /locus_tag="IV038_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP8340863.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(32055..33332)
                     /locus_tag="IV038_000621"
     CDS             complement(32055..33332)
                     /locus_tag="IV038_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340864.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(33411..34055)
                     /gene="pcp"
                     /locus_tag="IV038_000622"
     CDS             complement(33411..34055)
                     /gene="pcp"
                     /locus_tag="IV038_000622"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP8340865.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(34254..35198)
                     /locus_tag="IV038_000623"
     CDS             complement(34254..35198)
                     /locus_tag="IV038_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP8340866.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(35200..35886)
                     /locus_tag="IV038_000624"
     CDS             complement(35200..35886)
                     /locus_tag="IV038_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP8340867.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            36359..36679
                     /locus_tag="IV038_000625"
     CDS             36359..36679
                     /locus_tag="IV038_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP8340868.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            36840..37745
                     /locus_tag="IV038_000626"
     CDS             36840..37745
                     /locus_tag="IV038_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8340869.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            37742..38515
                     /locus_tag="IV038_000627"
     CDS             37742..38515
                     /locus_tag="IV038_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8340870.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(38692..40116)
                     /locus_tag="IV038_000628"
     CDS             complement(38692..40116)
                     /locus_tag="IV038_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8340871.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            40327..40773
                     /gene="nrdI"
                     /locus_tag="IV038_000629"
     CDS             40327..40773
                     /gene="nrdI"
                     /locus_tag="IV038_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8340872.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(40843..42825)
                     /locus_tag="IV038_000630"
     CDS             complement(40843..42825)
                     /locus_tag="IV038_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8340873.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGARQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(42839..43735)
                     /gene="murQ"
                     /locus_tag="IV038_000631"
     CDS             complement(42839..43735)
                     /gene="murQ"
                     /locus_tag="IV038_000631"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8340874.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(43738..44799)
                     /locus_tag="IV038_000632"
     CDS             complement(43738..44799)
                     /locus_tag="IV038_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8340875.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(45011..45538)
                     /locus_tag="IV038_000633"
     CDS             complement(45011..45538)
                     /locus_tag="IV038_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8340876.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(45605..47107)
                     /gene="lsa"
                     /locus_tag="IV038_000634"
     CDS             complement(45605..47107)
                     /gene="lsa"
                     /locus_tag="IV038_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8340877.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYRLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(47365..48486)
                     /locus_tag="IV038_000635"
     CDS             complement(47365..48486)
                     /locus_tag="IV038_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8340878.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            48612..49553
                     /locus_tag="IV038_000636"
     CDS             48612..49553
                     /locus_tag="IV038_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8340879.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(49696..50592)
                     /locus_tag="IV038_000637"
     CDS             complement(49696..50592)
                     /locus_tag="IV038_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8340880.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
BASE COUNT        14864 a        10467 c         8940 g        16521 t
ORIGIN      
        1 tctaagatgt cataaacatt aatattagga attgaaaagg aacgtggaag atccgaagtc
       61 gtgatgacca attttgtctt gtcaaaataa gtttcatcat tttggtcgat taattcacgt
      121 agttctctgt agtccttcgt gaaaacttcg atggattcat ccgataaatg atgacgaaaa
      181 acatctttca atttgtccga aatccccaaa cccgacatac aggaaatgat aatgttgcta
      241 ctttgagaaa agccttcaaa gtattgaaca ttgattgaat acttgtctgc tgcatcctcc
      301 gcaatctgtt taaatggcgc attcatcgtc atcttcaagc caatatcaag tgcaatggaa
      361 gtcgtcaaat tattcagaag aagtaactcc ccttttaaat cgttcttgat ctgtgtgtaa
      421 atttgattta aagagcccat atctacgatc agcactagac tatctgtatt cacttgccgt
      481 ttcacgaaat tccttgtcga ttcgacaatc tctgacacat tactctctac tggcatatcc
      541 aacgcttcaa aaacataatt gccgcataat tgattcacta ctgcttgaat gctgctagcc
      601 gtactatctc cgtgagcaag caacaggccc ttcagctcga tggattcatc tacataatca
      661 gataaagtca cgacaataag cagtcttaga aagttctcgt ccttttctga gagcggtggt
      721 aaatgctcca agaactgatt acataagtaa gtcgtccgag gtagatttgc agctaatgta
      781 cgatttatcc cggttaagtc tgtcttatag cttggggaaa tgacatagga tctgctaata
      841 atttctgcaa caggtccact cgtcgtcaat ccgtacgttt ttcctaaaac ggatacccag
      901 acctttctaa acatctgaat catcaatttt ctactcattg ataaaaaaga atccttatca
      961 atgtgatgca ccaaatgctg tacttcattc tttatcgaag aaaaattttc attgaaatta
     1021 ccttcattta tcagctgagt aattttttcc aaaatcgaat cataatcaaa cggtttgatt
     1081 cgatccgttt ttatcttttg atttttcttt atcaataaac tagcaatcgg caagctttct
     1141 acttcggatt ccagcatatt taactgaatc aagatctcat ttttcccaat cgttaatggt
     1201 tcttcttgtg ttcgactata cacatccgca caactgattt taatgatatt cttcagctta
     1261 ccaatattcc cactaaaggg atgagataac attaaagaca aggcttctct tgaaacaata
     1321 atatcttttt gtaaaacatc cgcttcattt tgaaaaaata attgaatcag ctctaatcgt
     1381 tctcgaatgg ggcgatcttc aaaagttggc aacataacac taatttgaat acgccgatca
     1441 aaggtgtcta ataaataatt ttcacctttt tccgtcgtgg cgaaaataaa acgaacctta
     1501 gcagaatgcc aatttacatt ttcaccgact ggacgatagt ttccagtatc aataaataga
     1561 aagagctttt cttgattttc tttcgatagg cgatggacct catcgagaaa aagatagccg
     1621 ttgttggccg ttgccaataa accatcggta tccttttcag cccccgtaaa ggcccccttc
     1681 ttgtatccaa acagtgttgc cgaaagcagt tcgggattgt ccgcataatc ggcacagttt
     1741 aagatagcaa atggtgcatc tttttcaata atttgctcat ggatagcgta ttcaaaaatt
     1801 tttgtggcta aaaaactttt tccaacaccc gtggctccat ttattaatac actgagtccg
     1861 ttaggcggat atttgacagc ggctatacat ttttgaatga cctctctcag acttccgtca
     1921 tgaccaacta cagaatgaaa ggcttcgttt atttgctccg tattatctac aaccgcatat
     1981 tcatctaaag gcttccacaa aacaggcttt cccggtaatt tctcgacctt tttatcttcc
     2041 actaatctcg tcaaataatg gctggcattc tgacgagaga tacctagatt cttcgaaatt
     2101 ttctcggcag tgacagaaga catattccct gttagtcgta tcagctcatc aaaaactcga
     2161 tctttagcgc tctttttcat tttttcctcc agtttaatgt gtctaaaagc ataataaact
     2221 atcggctgca atatgtctat tctatatttg tattccaatt tttactttga aacacaaata
     2281 gtcgaatatt tgcctcagat atctgacaaa ataatttatc taccaattta gtttttatct
     2341 aatatgattt ttatgttttt tcatagctac aaatattgaa ctggattcat atattagttt
     2401 tattaataga ttacagaagg gttaagtata tttgcatagt ttccaattac tttatccttc
     2461 tgtaactttt tcaatcttag atttactagc ccgtctcggg ccctatttta caatggtttt
     2521 gtccactttt ctctggtaga aaaacgtggt agaaaaaata aaataacaca aaaaaagcgg
     2581 cgtttcatcc gcttttggta gaagattgga aaactcagat agaaaaagga cgatttttag
     2641 ctctttagaa cgttgataag tccccattct tactgagttt tgccattatc tcctatacat
     2701 cttgaattct aagaataggt cattatagat gcctaggtat ttcgatccta gatctttgta
     2761 gacttcaaga tgggagatcg tgtcatcaga tggatagaat tgttcgtccg acgaaatgtc
     2821 ttttggcaat aacgcttttg cttcttcatt tggtgtagaa taaccaatat actctgcatt
     2881 ttgtgccgca ttttccggtt ctagcataaa gttgatgaaa tcataggcac cttctttatt
     2941 tttcacggtt tttggcataa cgatattatc aaaccaaaga ttggatcctt ctggtgggat
     3001 cacataatgt aaatgttcat tttcccacat catatcggca gcttcgccag aaaatgtcac
     3061 tgccacggag gcttcttcat tgatcatata cattttgatc tcatctgcca cgattgcttt
     3121 gacatttggt gttaaatgat tcaatttatt tgctgcttct gtcagctgtg ccatattctt
     3181 actattcaaa gaatagccta aactattcaa agaaagcccc ataacttctc gtgcaccgtc
     3241 aatcagcatc aaactgtctt ttaattccgg cttccacaaa tcatcccaat gcttaatttg
     3301 atttgcatca aagaatttat cgttatatac gatccctaaa gttccccaaa aatatggaat
     3361 cgaatactga ttatctggat caaaatccaa gtctaaaaat cgtggatcaa tattatttaa
     3421 tcctttgatc ttcgaatgat ctaatggaag tactagtttt tctttgatca ttttttggat
     3481 catatattca cttggtatag cgatgtcgta gtttgtccca ccttgctgga tttttgtgaa
     3541 catcgcttca ttagaatcaa atgtttcata attcactttg tatccatatt ctttttcaaa
     3601 tttcgtgatc aaactaggat cgatataatc tccccagtta tagatggtaa gtacctttgc
     3661 tccactcatg ccacttgttt tttcaagttg acgaacacca agcagcagca ccgcaataat
     3721 gatgagaata cctaaaaaca gagattgtaa ttttttcatc gcagccgtgc caccccctcg
     3781 cgtactcttc tgtttttctt cagccgtttt ggacggtttt ctttactaat aaagtagtat
     3841 ccaatgacca atatcatgga gaacaagaaa actaacgcac ttagcgcatt gatttctaaa
     3901 ctgattcctt gtcttgcacg agaatagatc tctactgaga gcgtcgtaaa gccatttcct
     3961 gtgacgaaaa atgtaaccgc aaaatcatca agagaatagg taaatgccat aaaatatcca
     4021 gctatgattc ctggagaaag ataaggcaaa atgatatttt tgactacttg gaaattatta
     4081 gcaccaaggt ctctagccgc atcgaccatt gaatcgttca tttcctgcaa ttttggcaag
     4141 accatcaaga cgacgattgg aatgctgaaa gcaatatggg aaagcaatac actctgaaaa
     4201 cctaatccga atcctagtag tgtaaagaaa atcaagaaac tagcaccgat aataacatca
     4261 ggagagacta ataagatgtt attcatgctc aacaacgctg tccgtgcacg tcgttttttc
     4321 gtgtaataga ttcccatagc cccaaatgtc ccgatgatcg tggcgatcaa tgcagataag
     4381 aaagccagca tgaacgtatt caatacgata atgatcaaac gtgtatcttc aaaaacagct
     4441 tcataatttt cccaagtaaa tcctgtaaag ctgttcatcg ttcctccttc attgaaggaa
     4501 taaaaaatca gatagaagat cggtgcatat aagaataaaa agacgatccc taaataaaga
     4561 tacgaccatt taaatttttt catttatgag cgacctcctt ttttcctctc gcctgtcagg
     4621 agcatcacga tgaacatagc aatgattaaa atcacaccga ttgtagaacc catcccccag
     4681 ttttgtgtaa ccaagaaatg ctgttcgata gccgttccta aagtgatcac ccgatttccg
     4741 ccaatcagac gagtcagcat gaataaagaa agagaaggaa taaatactgc ttggacacca
     4801 cttttcacac catttaatga taacggaaaa atcactcgtc ggaaggtctc aaagctattc
     4861 gcgcctaaat ctctactagc actgatcaaa gaaggattca tctcttccaa tgcattgaaa
     4921 attggcataa tcataaatgg aatctcgata tacgttgcca cgaacagaaa actgaagtct
     4981 gtgaataata tctgtttcgg cccgatcccg ataaaatcaa agaaatgatt cacgctgccg
     5041 tgcacactga aaatcccaat aaatgcgtaa gctttaagca gcagattcac ccatgtaggc
     5101 aaaataatca gcatcagcca taattgcttg tgcttcagtt ttgttaaaaa ataagcagtc
     5161 gggtaactga tgatcagcgt gaaaaacgtg atcaaaaatg cataccatac agaattgatc
     5221 gtcatagaaa gcgatgtccc cgacgtaaaa taagatagat aattattcag cgtgaattgc
     5281 ccattcatat caaaaaaaga ttgataaatg atcattaata caggcgcgat cacgaataaa
     5341 ccgagccaca aaatataagg gacggaatag atcctgcgca ttgttttcat tcttgtcccc
     5401 cctattcttc gtagctgtca agacgtgcat cgaattcttc ttcagactcg ttgaaacgca
     5461 tgacatggat atcttctggc tcaaaggcca atcctacttt gcttccttct ttagcttttt
     5521 tcgttgaatg gaccatccat tcattttgct gttcatcata acagatgatt tcataatgga
     5581 caccacgaaa cagctgagta tcgaccgtta cgactagttt tcctttatct ggtgtcgtga
     5641 tcgtcaaatc ctctgggcgt aaaacaactt caactggttc atttagacgc atcccgccat
     5701 cgacacattc aaattgctta ccaacaaact caactagatt atcttcgatc attacaccgt
     5761 ccacgatatt gctctcccca acaaaatcag caacgaaatg attgattggc tcatcataaa
     5821 tatctacggg tgtaccactt tggacgattt tccctttatt catgacaaaa atctcatcac
     5881 tcattgccaa cgcttcttct tggtcatgag taacaaaaat aaacgtgatt cctaatcgtt
     5941 gttgcagatc ccgcagttca tactgcatat ctgtacgtag tttcaaatca agcgccgata
     6001 acggttcatc taatagcaat actttcggtt catttacaat tgctcgtgca atcgctacac
     6061 gctgtctttg accaccagac atttcactaa tctcccgttt ttcgtatcca ggaagctgga
     6121 ccatacgcaa agcatctttt accttctttt cgatatctga tttggacatt ttcttgatct
     6181 tcaatccaaa agcaacgtta tcaaaaacat tcatatgagg aaaaagtgca taatcttgga
     6241 agactgtatt tacttgtcgc ttattagctg gcaaatcatt caaccgtttc ccttcaaaat
     6301 agatgtcccc ttctgtagct tcggtgaatc cagctataat acgtaggatc gttgttttac
     6361 cacagccgga aggaccaagc agtgtataaa attttccttg ttcgatctca aaacttactt
     6421 tttttaatac tgtttcatca tcatagcgtt tcactacttg atcaaatgaa atgattggtt
     6481 tccccacgtt tttaccccct atattacaaa tatgattgcg ttgcaacaat caatactttt
     6541 gttcgttttt tgagatgatt ccttaattga tgttgttttg tcgcttgata atagatcgac
     6601 tgtcctgctt ttgccacata gaccgtctct cctaagctta actctaattc cccttctaat
     6661 acaaaaataa acgtttcaga taatgaaggt tcaaacgtct tatattctcc tgcaccttca
     6721 aaagtcagca agacaggctc catttctttt tcattcgaat cagccacgag ccattccaat
     6781 tcatacccgt tttcttcatc gaaataaacc gtatgatctt cttttgaata aaccacttgc
     6841 agattcgctg cttcttgatg aaaaaattct tctggagtca cacctaagac ttcaagaata
     6901 gaaaagaatg tttccattga tggtgaactt aaatctcgtt ccaactggga aatataccct
     6961 tttgacaaat ctgtgcgttc gcctagttct tcttgtgtca aattcttttg gatgcgcaaa
     7021 tttcttaact tctcaccaat ttccataatt tcctccgaat cgaagttttc taatagtaaa
     7081 cttcctgttt actaccactc gtattagtat acgaaattta ttgaaaaagg caaccatttg
     7141 taaaattttt tttgctgaat atttctttat cttttggaat aaattctagt tgtttgattc
     7201 tttatttttc acctaatttt tgtctaaatt tacggaagaa caagaataaa agagtaggct
     7261 ttatggtatg ataaaagaaa attttaaaag gagtgtctca cgaatgaacg cttttcgatc
     7321 ttttcaggta ataaaggaac ctgttttcca tacagcaatc agtgatctgc catttactga
     7381 gttggctgaa aatgaagtct tggtaaaaat tgcctattct gatgtcaatt ataaagatgc
     7441 attggcaatg agcgagtctg gtcaggtgat acgaacatat ccgatgactc cagggattga
     7501 tttatccgga actgtcgtcc aaagtaacaa tccacgtttt tcaaaagaag atcttgtact
     7561 tgctactggt tttgggcttg gcgtcaccca tccaggcggt tatagccaat atcaaaaagt
     7621 accaggagac tggttagttc ctctaccaaa aaatatgacg ctgcgtcaag cgatgatcct
     7681 tggtacagca gggtttactg ctatgttgtg tgtgaacgct ttaatccgtc aaggtatgac
     7741 ctccgataaa aaagttgtcg ttactggtgc ttctggcggt gtaggcagta cagcgatagc
     7801 cctgttacat aaactaggat tccagtcaat cattgccttt tccagaaaag aagagtctgt
     7861 cacatggcta aaaagtctag gtgctttgca agtcgtacga cctgatgaat ttctgccaga
     7921 gacaacaaaa gctttaggaa aacagcaaat cgactatgtc atcgacacag taggtggcga
     7981 gcagctaagc agtcttcttc cattgatctc ttataacggt gcagtagctt tatgtggcaa
     8041 tgctggggga atcaaactaa acgcgactgt cttgcctttt attctgcgaa acatccagtt
     8101 gattggaatt gattctgtca atgtaccaat agatcaacgt ctttctttat ggcaacagat
     8161 ggcggatttg cagattgcag atgaactggt cgtccaagag attactctgg atcaattgcc
     8221 tgaaacagca tctaaactac ttgctggaac acaccaagga agaacactag taaatgtagg
     8281 tgaccataaa tgaagattct aatcacagct ggaggaacga ctgaaaaaat cgatcaagta
     8341 cgtgctatta caaatcattc gacaggacga ttagggcaag cattagctga ttatttagct
     8401 gccaatccgg ataccacaat cgattacgtg actacgaaac aagcactgaa accgaaaaga
     8461 cactccaata taacgattta tacgatcgaa tctgcactgg acttattttt acaattagaa
     8521 gaactaacga aaaaagaaca ctacgatgcg attatccaca gtatggcagt cagtgatttt
     8581 actcctgctt ttagtttttc agaagaacag ctggcaaaaa acctacccac ttcttcaacg
     8641 caagaagaac tggacaattg gtttgcggaa aatgaacaga cagataccac tgtttcaaag
     8701 atctcttcta acaccgagca tcttgtgctc gttttgaaga aaacaccaaa aatcatttct
     8761 tatctacgtg aatggcagcc aaaagcaaaa atcattggtt tcaaactatt agtagatgta
     8821 ccaaaggaat ctcttctggc agtcgcaaaa aatagtctga taaataacaa aactgatttt
     8881 attttcgcaa atgatttgac ggaaatccat ggcgagacac accacggcta tctcttatct
     8941 aaagatggaa cagtggaaga agctcaaagt aaatctgaga ttgctgcatt gattactgag
     9001 aaaattagac tggaggaatc aaaatgaaaa agatcttact aggcgttact ggaagtattt
     9061 ctgcttacaa aagcgctgat cttacaagtc aattcgtcaa attaggtgcc caggtagatg
     9121 tgattatgtc taaaagcagc acacatttta tcacgccatt aaccttgcaa tctctgtcga
     9181 agcgcgcagt ccatacagac gtcatgcaag aatcaaatcc gcaggtaatc aatcatatcg
     9241 aactagcaaa acaagctgac ttgtttctca tcgcgcctgc tacagcaaac attatcggca
     9301 agctagcaaa cggcattgca gacgatcttc tttcaacagt cgcaatggcg cttccagtgg
     9361 gaacaccaaa attgattgcc cctgccatga acacgaatat gtatcaaaac ccgatcactc
     9421 agcgtaatct ggaaacattg agatctgtcg gctatcagga aattgaacca agagaatctc
     9481 ttttagcttg tggagatttt ggaaaaggtg ccttagctga tattcagacg atcatcgaac
     9541 aggtcatgct gactcttaat gaaagaaatg cttagaaaaa aggagtactt atgaaaaata
     9601 caaaaaactt tacattaact gcgatgtttt tagctatttt gattttatta gctgttacgc
     9661 ctttaggttt tatccctatc ggtccaatca atgcaacaac catgcatatt cctgtaatca
     9721 ttgcttcgat cgttttagga ccaagactag gtgcttttct tggcggtaca tttggactga
     9781 tcagcatgat ccgtagcaca gttatccaaa cacctttgtc ttttgtcttt tctccgttta
     9841 tcccagtcat cggaacagaa catggaagct ggaaagcctt gctgatcgcg tttattcctc
     9901 gcatcttgat tggggtggtt ccttatttcg tttataaagg tctgcgcaaa ctaatgaaac
     9961 aaaaagcaga ttccgtttct ttattcttag ctggactttc cggttcattg accaatacga
    10021 ttttagtgat gaatatgatc tactttttat tccagcaaga ttatgccaaa gtcatcggaa
    10081 caaatttaaa tgctgtttat tcagctattc tagcggttat ttttacaagt ggtatacctg
    10141 aaggaatcgt cgcaggtctt gcgactgcag cagtatgcaa tgtcttgtta agatacttca
    10201 agcacccttc tcttactaac tgatagtcat cgtagccgat ctctgatttt tttctctttt
    10261 ggtgttacta ttgaatggaa agatttgacg tcgaaaggat gatccaaatg aaaatttttg
    10321 ttgtaggtgc aaacggacaa attggccgac acctgataaa agatttagct ccaagttccc
    10381 atgaaatttt tgcaggggtg agagatgttg cttcccaaac attagtaaaa aaagagaatg
    10441 tttcgtatgt ctcttttgat ttaacttggt ccgtggaaaa aatgaccgaa gcattcaaag
    10501 gcatcgatgt attgattttt gctgccggct ctcaaggaaa gaatcttctg caagtagact
    10561 tggatggcgc aatcaaaaca gtgatcgcag ctgaaaatgc gcatgtctct cgctatctga
    10621 tggtcagtgc tgtttatgca gatgaaccag caaaatggcc tgagtccatg acagattatt
    10681 atattactaa acattatgca gatgaatggc tgaaacgaac taacttagat tttgtcattc
    10741 ttcaaccagt cacattgact aacgatgaag aagttacttc cattcaatta actaagccta
    10801 atgagaaggc atcaaaaacg atcactcgct ctacagttgc tgctgtactt gccgctctag
    10861 tggaagaaac ggatatttct cgtacgactc tagtactttc tgaaggtagc aaagagttga
    10921 atacagcatt ccaagaatgg gcaaaggagg aatagatcca tgcgtgccat ttcaatcaag
    10981 catcctggtt ctgtagatgt attagaacaa attgaagtgc ctgttcctaa accaaaacaa
    11041 ggcgaattac tgatcaaagt ccatacagca gctgtcaatc gaacagatat tatgaaaaga
    11101 gaatcagtct ctttgaaacc tccctaccct attttgggag tcgaagtagc tggagaggta
    11161 gttgagaata aaagtgatat ctccactttt actcctggga caaaagtata cggattagtc
    11221 aatctaggtg gttatgccga atatgctgtg atgcctgccg atcgtgcaat ccttctccct
    11281 gatacgctag actatgtttc tgctgctgga atctcagaag tttttttgac tgcttatcaa
    11341 acactttact ggcttggaaa attacaagaa ggcgaaactg tcttgatcca tgcaggagca
    11401 agtggtgtgg gaacagcagc gatccaatta gctagacagc tttctcacgc gaaaatcatc
    11461 gttactgcag gatcagaaga aaaactcgcc ttttgtaaag aacttggtgc agatgaattg
    11521 atcaactata aaaaacaaga ctttcaagaa gaggtacgaa aaatcactga aggtcgggga
    11581 gtcgatgtta ttttggattt catcggcgct tcttactggg aaaagaattt agcttctata
    11641 gccgttgatg gacgttgggt cttgattggt atgctaggtg ggaccatcgt gcctgagata
    11701 gaccttggtg tattgattgg gaaacgtgtc caactgcttg gtacattatt gacacctaga
    11761 agtgatcagt ataaagcaga gctcgcacaa acttttatgc aaaaagctgg gccttttcta
    11821 gaaaatggca agattcggcc aatcattgac cgtacattcc aattcagcgc agtcaaacaa
    11881 gcgcatgaat atatggaagc aaacaaaaac atcggaaaaa tcattttgaa gataactgaa
    11941 taaaatcatg ctgatcaaaa aagaggctgg gacagaagcg tttaactcca agaaataaga
    12001 agaaattcac gaaaattgct tttcaaattt ttgtgaattt cagcttattt ccgaaggaat
    12061 ttcttctgct tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt
    12121 tgtcccacgc tctttttccg aataaacggt gttcaaaagc ttgcatctgc ggtaataagt
    12181 ccaaccaagt taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca
    12241 gggcagcttt ttcacaacct cttcactaaa cagtgttcaa tcagctgacc ctcggtatta
    12301 gctcaaaacg ctgatttcac aacctctttt tatttacgtt ctatttcatt cattttccga
    12361 agcaaatcga gctgcttgta tgcccgcttg tcgcccaaaa ataatgatct ccgcaactga
    12421 atttccaccg atccggttgt ttccatgtaa gccacctgtg acttcaccag cagcatataa
    12481 ccccctaatt ggctggtttt ctttatctaa tacttcagtg ttcgtattga ttttcacacc
    12541 gcccatcgtg taatggattc ctggtgcgat tttgatcgca taataattcg gagacgataa
    12601 gtcatgttcc atggctgtgg cacggttgaa ttgagcatct tttttctccg caacatccgt
    12661 attccatcca tcaactgttt ttgctagtgt ttcttttggt aaatcgattt tttctgctaa
    12721 ttcttccatt gtctttccag ttgtgacata tcctttttgg tcataaaatt caatagctgt
    12781 ggcacgatca cgcacgcctt ggtcaaaaat caaataagct gatttttcag gcaatgctgt
    12841 aattgctgca gatactttat cccttgtatc catttcattg acaaatcttt tcccctcttg
    12901 atcgaccaag attgctcctt ctccgcgaac aacctcacca atcaagaccc cctcatcctg
    12961 ctgcacagtt ggatgaattt gtattttatc catatcaacg gtttgtccac ctaatttttc
    13021 aatcatggtg ataccatctc cagttgatcc ttcttgattt gtcgtaatat aatcagctaa
    13081 atcgggacga tatttttcaa taaattcttt gctggcacca aaaccgcctg tggtaactac
    13141 gattgctttt ccggtgacct cttttggttc ttctccttga acatgtacag tcacttcatt
    13201 gaccgtttcc ccatttttct taatgtctgt cacatctgcg ttgacaaaaa tagggatttc
    13261 ccgctcatgg acgttattca ataacccctc cactaagtat ccaccaatag ccgagccatc
    13321 agacggacga tgtgtcctct tttcactcat ccctccagtg atcgttaagt tgtccagttt
    13381 gataccgttt tgatctagcc aatcgaccgc atcagcagag tgatcgacaa agtatcgaag
    13441 catttcttta tcattcgttc ctccaccacc ttttagcgtt tcttcataga aggcatcatt
    13501 cgagtctttg attccttctt ctttttgaaa ttttgtttct gaagcattca taccgcttga
    13561 tgatttagat gtgtttcctc cggcaacagg catcttttcc aaaattactg gattcaaccc
    13621 agcatctttt gcttcgatag ctgctgccat ccctgcacca ccagctccaa taatcacgac
    13681 atcataagat tctttcattt cttttggatc tgtatatact tgttcagaag cgcctgatga
    13741 tacttcggca gattccttcg tactagaact tgtctgcgct gtatttttct gatctgcgcc
    13801 gcatgcgctc atagcaagaa gtgcaacggc tgttaataaa ccagcaacta attttttcat
    13861 ttgtatcccc cttttctcct tatactatat taagtgattt ttttcacaat atcaactctg
    13921 tttttcttta agtcatatag ctttggtaaa cacacgcttc ttagtataat taaggatata
    13981 catgaaggag ttattttatg aatcatttga aaaccttttt tacgaacagt acaaaaactg
    14041 caggtagaat cacaaacgga atctatattt tattacgtat catcgtcgtt attatgctag
    14101 tggatcaggt ctggaaccaa cactggggaa atacaatgct tttattactg acattaattt
    14161 tatttgaaat cccttatttt ctggaaaaga tattaaagat acagattcca aatatgctag
    14221 aaatcatttt gttatctttt atttttagtt caacagtttt aggagagtta agtgattttt
    14281 atggctattt ttcaatgtgg gatacttttc tacatgcatt cagcggattt ttagctgggg
    14341 gtgtcggctt ttcccttgtt tatttgctca ataaaaatac aaaaaccatg aacttgactc
    14401 ctttattact agcagttgtt tccttctgtt tttcgatgac agttggtgtt atgtgggagt
    14461 ttgtggaatt ttcagcagat aaattactgc aattcgatat gcaaaaagat acgatcatcc
    14521 agcaaatcaa tagtgtggag ttaagtaaag acggaaacac tgtctatcat gttccagata
    14581 tcactcggac aatcattgaa agtcaaaatg cagatggtaa aacagataaa acaatcatcc
    14641 aaaatggtta cttagacgtg ggaatcatgg atactatgaa ggatttattt gttaatttta
    14701 ttggagcagt tgttttcagt gtatttggtt acctctatag tcgaaacgat taccaaaaat
    14761 ttacgttcat caagaatttt attcctaaaa aaatcaaacg ataaaacatg ctaaaaaaca
    14821 agagaagggg cagtgacaat acttttgtca ctgccccttc ttttttgaat gtatggcatg
    14881 ccattagtta tccgatagta ttaactcaaa aaagtaagat atgaggaatg actatttcgt
    14941 tttttatccc aatactgagg acttatttca ccatttcgtt atttatctaa tttacttaaa
    15001 acctgcataa ctttttcata atctggatta ggtgtgccgt ctagattttc agcaataaaa
    15061 gtaaaaccca tttttttata aagagcaatc gcctgatggc tccatgtctg tgtgtgaaga
    15121 tagattggtc cgtccgtatg gagggtagga aattcttgca agactctggc agtcaatgct
    15181 tttgcgagcc cttttccttg gtaatctggt ttcactgcta accaatgaaa aaccggatac
    15241 acttgctctc ttgatttttt tcgccaagcg gtacaggttg ctactttttc tccttttttt
    15301 gtctctacaa aaaacatcct tctttcaagt tcttcaggat aaggagcaaa gttttgttga
    15361 aaatactcaa gtgcttcatc tgtcgtttga aattccccaa cggaagtctc aatctccgcc
    15421 caagcagatt catcttcttt ttgataaaat cgaaattcaa aaccttccgc taaaggcgtg
    15481 attggaacat tttgttgaag tggtcttgtc atccagattt ctgcgtaagg taacgtttta
    15541 tctaacattt ttctatccct cctcgtctcc attatgccaa ataacatatg actcagctac
    15601 ttatttgact ataacagata gagatataaa tcagaaccag tcatctgcgt caaattcatt
    15661 tcgattagcg tatacccatg ttcctgccat acctcgcatg aaatctccta aagcagaaga
    15721 aggaatacct ccaccaggtg cgggtgttcc cggactagga agtggtactt gcggctgcag
    15781 atgctcatcg gtggaatcaa tagttgacaa gcgttttttc ttcgtttcct tctggtcatc
    15841 tgattccttc tgatcttccg actgtttagc aacaaattct ttttctgatt gttgttttat
    15901 tttaggctct aaaatttcat tttccaatcc agaaacttgt ggggccaaat gagcttcttg
    15961 ccttatcgtt ttattagatt ctgagatttt tctagatatt cttgctaact ctaggcttct
    16021 tgtttgtttt actttccctg ttggagaaac tgcatgggcc atttgttgtt ttttttctga
    16081 caagctttct tcttgtcttt tttgttcttt ttgatctatc tgtttgttta atggtaaaac
    16141 atgtaatagt gactgagcat ggagttcttg gagtgaatac gggtcatgat aactatacat
    16201 aatcgtaaaa tcacctgttt gtttggtcaa ataaccatcc tccaatggta cttcattgat
    16261 tgtaaccgat acatccttta acggtacggg tttacctaag gaatttgttg ctgattggat
    16321 caactcttct ttgtttattt tttcgtttgg atgaacaata agatcatgac cttctaatga
    16381 acgattgtct ggtatataaa cataagtaaa ggtaatttcc cctcctgatt gcttcctatt
    16441 attcatcagt tctgagtgaa gtacttcaag gatctccgac cagtgacttt ttctgtctaa
    16501 tccgtttgct tgcatccaat cagaaataga agtgactttc ccattttgtt cataagtcga
    16561 taaatctata tttggcactc gatcagaaaa ttcagcaggt aggcgcaaat caggaatacg
    16621 atttacacta ctcatatgtg tttctgcttg aagttcttta tttgtttgac tatctataaa
    16681 atgagtgatg acagtcgatg aagcagcaga tgcttctaca taaactacag ctgttgtttc
    16741 tgccacattt gatgttttcg agtctatata agttaataca acgggatatt ctcccggagt
    16801 tgtagtatct actgtcccgt tcattttgat ttcttcttta tttcctgaag ttccatcgct
    16861 ttttgttaca tattcaacaa gatctttagg ggcgtaactc tctcctgttc ttaagtgaag
    16921 atcttttcct ttgatagctg tttgatcagc acgaacatgg atcacagcag atgcagtcat
    16981 tgtttttaaa gcaattggat caaaatagga caaggaaata gtagatgttc ctgtattagt
    17041 cgtatccacg atattcccat tgtttgaaat tactttggac atatctcctt gtgttccatc
    17101 tgcgttcttc agcctttcta ccagatcaga tatcgaaact tttgtccctg ccagaacatc
    17161 gattttcttt ctttcaaact gtgaataatc tttttcatat acataagaaa cttgtgcgct
    17221 tccaccgtcc tttatatctg tcaaagcatg attcaaaata tcagtgatct gttttaactg
    17281 atcacctttt gtaggatcag tcatatcgga aaagatagaa agcggcacac tgcttccggt
    17341 ttccgatgtc cataatgttt tgttaggaat taaccagtag tggtttatcg ttggaggata
    17401 actgacttta ttataggaag tcaatgcagg aagtagctgt tttccatttg catcagtgaa
    17461 agtatgagta atagtgatag gggaatcagt ggtctcagaa acagaggtac gtacgacctt
    17521 gatggtttgt gtcgttgcct gaacgggtac atgcatatag acacctacag ataaactgat
    17581 cagcatcgtg cgcatcataa acttaacgta gtgtttttca gaacacgacc gttccttatc
    17641 taaacgacaa gcgtgtttat ttagttgttt tgaacgtctt tttgaacaca tttattctta
    17701 gtcctcccta ctcttgtcta aacatgaagt agtaattttc ataagatgat ttcattatat
    17761 aaataaaatc aaaagaagat ggactattag gagagtattt tttttgattt tttattgttt
    17821 caagaaaatc ggaataataa cttatgtact ttttaatttt gttaagtaat atatctagtt
    17881 atatctagtt atatctagtt accttttctt tgaaaaaaac ataaatcagc taataaatat
    17941 gtgagacaaa gctgccatcc ataaatacta gctgattttt tcagttaaat gtctattttt
    18001 ttctctgatg atggtatttc caaaatattc ccagtcatct ggagtcggtg gatttttcca
    18061 aattatctgt tctttattaa atttttcaag taaacaatca gataaataaa tattcgtacg
    18121 agaagtgatt cgactctcaa tcacaatatt gtaattttta aatcctttca cttgagaaat
    18181 aatgtaatcg gtataagcag atccttgcga aaaatcaata cagacatgaa tcggtgcttc
    18241 aaaatatgat accggacaaa taccgaccaa taaaaagaga taatcataaa ataagcgaat
    18301 catgaaacgt tcatcttttc ccaagaaata gcgtttattc tcttgtatga ccttttggat
    18361 tgctgtactg aatattggat acgtctcgaa gaaaaactgt agctgctttt tagacgtaaa
    18421 gctagtcatc tcaaagtcga atatccaatt ttttcgattg acatgcatca tacccatacg
    18481 aagttctttt cgtatatata gtctttcttc ttcagagatt tcaatcgaaa atggtaattg
    18541 atcaatcaat tccactgaca ttttttgact aatcatgtgg atatcatgaa aatgagcttc
    18601 cgcaagtcca aatgcgccat ctaattcaaa ttcttcttcc gtgatcaaaa acaacatgat
    18661 ataagaaatc tcatcaatga tctgctcttt agggatatga agaaaagaaa tatgtgataa
    18721 atccttgatg atcttcattc cttctgagct cttaaagtat tcatctgaaa agaaacgttt
    18781 tccgattggt tgattcccat aggccctcaa caaactaata gtggttagca aattgaattt
    18841 gattcgtttt gttgcagaca gttttaaatt gaacagatat tcaaaatatt gcacgatact
    18901 ctttgcttca ttcatgacgc tttctctaaa tggcatagaa aaaccattaa agaccccgaa
    18961 atatacccca aataatacgt tccgtatgtt tatttcttcc ccaactaata tgtttttctt
    19021 taactttatt tgatgctgtt caagaaaaga gactaattct cttcttttta cataggctct
    19081 tgatatgctt aaaaaatgat tctctgcaaa tttttcgatc gttacttttg aatccacgat
    19141 ctccgttaac agtcgagcaa tttttgatcg ttgaaagtag attcttctta aatttttgat
    19201 atccgtcaaa ttcagacgtt cgacatggat ctctgcttct tctgaaattt ggatctctgg
    19261 ctttgttttg aattcagtaa attctttatt taattctatg atataatttc ttgttttaaa
    19321 tttagacaaa cctaatgttt catataactt aggcagcact acataattct ttttctcaaa
    19381 ttctaaatat ctagctagca gcagtttttc tctatcttcc tcttctataa ggtaatccaa
    19441 cttcatatcc tacacctcaa aaaatgcctc ataccaatta cttgatatgc agatgaaagt
    19501 tcatcttttc acctatactg attttatgat ggattatgct tgttttttca tcagttattc
    19561 tcatttttta tctttttttt gagaatattg caaaaaatgg aaaaatttat gaggaaatat
    19621 tctatatttt ttgttttatt gtgttaaaat gtacaagatt tgcgccattt gcgtaaacaa
    19681 ccacaaaaaa gagcaggacg gccaccgtcc tgctctttta cttattttaa gttccattac
    19741 ttattaattg gctaaacttc tgcttgtttc tgatattgct tgtttttctc caatgatccg
    19801 cacttctgtt tctaaagaca catcaaattt ttctttgatt acttcttgga tgtgcgcaat
    19861 caattcaata taatctgtcg ctgtcgcatg atcgatattg acgatgaatc cagcatgttt
    19921 ttcagagact tgtgcaccgc cccatttcag gccttgcaat cctgcttgtt ggatcagctg
    19981 acctgtatag tgtcctactg ggcgcttaaa gacacttccg catgaagggt attctaatgg
    20041 ctgttttgat tgacgcaatt cggtcaactc atccattctt gctttgattg cttcataatc
    20101 ccctgtttgc aaactgaatc gagcttccaa tacgatagca cgtaattctt ggactttact
    20161 gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt ttcaatgttc catcttcaag
    20221 caaaagatcc acttcagcaa aaacatcttt gatctctccg ccatacgcac ctgcattcat
    20281 ataaaccgct ccaccgacac ttcccgggat gccgcaagca aattcaaatc cagtcaatga
    20341 ttctgccaaa gctgcataag tcgtatcaat cagtgaggca cctgcttccg caatgaccat
    20401 cgttcctttg acatgaatct gtttcatttc agtcagcatg atgactacac cgcgaatccc
    20461 gccatcttga acgatcagat tgcttgcatt tcctaatacc atccaaggta tatcatggat
    20521 tcggcaataa tcgatgattt gctttacttc ttcttgtttt ttcgggaatg caaggacatc
    20581 agctggtcca cctgttttcg taaacgtata gttcatcaat ggttcatcaa acaatatagt
    20641 aatcgtggct aagctttcaa ctatttcttc tttattcact gaggattttc ctttcttttc
    20701 tcaaacgtcc tcattattct atcacgctta atacaatctt gccagtcttg ttcaaagttg
    20761 atgacggtag tgatctttaa gcacttcctc acataattca tgacttctta caacttcttt
    20821 ttgtttaagc aattctgttt ccgatttacc taaacggatc tgagaaaaga aggcctctac
    20881 cattggctca aacccgcgtt tgactagcgt gtttgtccag tcgccaaatt ctttcacttt
    20941 ttttccatca gcatcttgaa tgacaagctg ggtcagatcg tttacttcat aagtaccatt
    21001 tttacttgtt acttgatatt tttctatatt agcgccactt gctaaatcca tcgttaaaat
    21061 agccgtttga ttttgtgttt ctaattgtaa aatggcatat tccaaaaatt cctttgtttc
    21121 tcttatctgg tattttgttc tcaaaatggg ttcatctaat aaatacacag ctgtatcaat
    21181 aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt tcttttgccg ctattcgatt
    21241 tttttgcagg tggatcaacc gcttatctgg gatacgtttc aattcttcta ccataggagc
    21301 aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc ttatcagcta attcttgcaa
    21361 ttcacgaact tcatttagat ggacactcag tggtttgtca atataaacat ggataccatt
    21421 ttctagacat tttttggcta attcataatg tacttttgtt gctgcgtgga tcatacatgc
    21481 ttcgattttt tcttctaaaa gctcatcgat cgtttcatat agatgttgga acccgtactc
    21541 tttttgaatt ctttcttttg tttctttatt tcttgttgca aaataaaaat caagtgttcc
    21601 ttgttttttc gcataagttg gtaaatatgc tttttctgcg atattcccta caccgattac
    21661 accaatcttc atctactttc ctcctggatc atttagttac tttcatctta caccattttt
    21721 tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa ttaattcgaa ggagtgtgct
    21781 tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt gccatcgtaa ataaaaattt
    21841 tttagaagtt tttcatgagt tagacgccga ttttttctgt ctgcaggaaa cgaaactcca
    21901 agcaggacaa attgatttag atcttccagg ttattatcaa tattggaatt acgcggaacg
    21961 aaaaggctat tccggcacag cgatttttgc taaaaaacca gcattgaatg ccacttacgg
    22021 aatgggtatc gatatccatg atacagaagg ccgtctgatt actttggaat attccgattt
    22081 ctttcttgta acctgttaca cgccaaattc tcaaagcgaa ttaaaacgtt tagattaccg
    22141 attagaatgg gaagaagcct tttataatta tttggaaaat ctaaaaaaac aaaaaccagt
    22201 catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt gatttgaaaa actggaaaac
    22261 gaatcaaaaa aatgctggat tcactccaga agaaagagcg gctctttctc gactgttgga
    22321 taacgggttt atcgatacat tccgttattt ttatccaacc caggaaggtg tctactcttg
    22381 gtggaactac cgattcaatt caaggaaaaa caatgctggt tggcggatcg attacttttt
    22441 gacgagcaaa gatttagagc caggactagc tgatgcaaag atccatacgg acattatggg
    22501 cagtgatcat tgtccagtag aattagattt gaaataaaga ggttgtgaaa aagtcgttta
    22561 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acattttgca taaatttggc
    22621 ataataccga gaaactggct tttgaacact gtttatcgag agattgtgaa aaagctgtcc
    22681 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg
    22741 gcttattacc gcagatacaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg
    22801 cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt
    22861 tgggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt tgtgaaaaag
    22921 ctgcactgca tcaagtaata agaacagcca aacaaaaata gttcctcata tttttcgttt
    22981 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattgaga agttgtgaaa
    23041 tcagcgtcct gacctgagta ttagagaaac aaatggcaaa atagttcctc atattttgct
    23101 attttttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gaagttgtga
    23161 aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa aatggttcct cacaactttg
    23221 cataaatttg acttaatacc gagaagtcat gatccccatc taactccaaa tcacaaggaa
    23281 tgagttcaaa aactagcttt tctcaacaaa tattacataa aaaattctat gttttttgtt
    23341 aaatttattt gcttttttca aaagtatgtt acagtaattt cccaaggagg ttttacagaa
    23401 atgaattttg ttgccatgga ttttgaaact gccaatcatc agccttatag tgcttgttct
    23461 cttgcgctag tcatggtaaa aaatagccag atcgttgatg aattctatac attgatacag
    23521 cctgagactc cttttttctg gcgaaatgtc caaattcacg gaatccatca agaagacgta
    23581 cgcaatgcgc cgaaattccc agaagtctgg caaactatcc aaccatattt tcaaaaaaat
    23641 cgtttagtcg ttgcacataa tgctgctttt gatacaaagg tcttagcggg atgcttagat
    23701 tattatcatc ttgaacagcc taattatctt tctttatgta cagtgaaaag tagccgtcgc
    23761 ctgtttcctg aatttccaaa tcatcgattg aacactgtgt gcgaaaattt gaacatccaa
    23821 ttagatcatc accatgatgc attggaagat agtcgtgcat gtgcggaaat cctgctttat
    23881 caagaaaaaa aattcgggac agatccattg aaaaaacttg tgactataaa gtaaaagaat
    23941 acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc actccgagtc acaaggagca
    24001 atttcaaaaa atagcttctc atgtttatta aatattgtga ctggtgaccg aggagctggc
    24061 aggagaacac cgtttattgg agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    24121 cagccaaaca aaaatagctt ctcatatttt tcatttggtt ggacttatta ccgcagatgc
    24181 aagcttttgg acaccgttta ccaagaggtt gtgaaatcag cgttttgacc tgagtattag
    24241 agaaacaaac ggcaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaaggt
    24301 cagctgattg aacaccgttt attcaaaaaa aatggactgc gacacgactt ttgtcacagt
    24361 cccttcgtgt gtttttctgc cttctcaaaa ggatgacagt ttctcactct tttcctattt
    24421 cttctattca atcaaataac tcattttata tacatctaaa aattcaccta gatctgttct
    24481 tgcccctcta ggcatgaccg cttcgatttg gaagcccatt tttcggtaaa ggtgtacggc
    24541 tctttcatta cgaacttgaa cgtcaagctc caatcgaaaa agtacaccca tttccttcgc
    24601 ccaactgatg atttcttcta gcataagagt gcctagtccc attccccaat attcctgcag
    24661 aatactgata ccgacttctc ccacgtgaga caaacgaaat tgactgtctg ccttgacaga
    24721 tgctgttcca ataatcgtcc cttcatagat tgcaagcagc aataaattat tattgctttc
    24781 gtatatataa tccaactctt cttctaaggt ttctggtggt aacaggagtt ctgcttcatc
    24841 caaaactaaa aattctgtct ccttattaac ttgtcccatc atcaaaagaa tgtcttttgc
    24901 atctcctggc actgcttccc gcaagatgat cccaacttct tcactcatga ttttcagccc
    24961 atttttctag aattcgctgt ttgaaggttt cactttgctc cccgtatgag attagtttca
    25021 cataccgctt ttctaaatct gtatcacttt tttctaaaat cagttccaaa taatcctccg
    25081 gcagtgcttc ttctaataga tttccccatt caacaacgga aagcccatca ccttcaaaat
    25141 attcatctaa ccctaaatct gctccgctag ctgctacccg ataaatatcc atatgataca
    25201 acggaatccg accttgacta tattctctaa taatcgtgta tgtcggactt ttgatcattt
    25261 gcgttatccc taatccttgg gcaattcctt tcgtcaacgt tgttttaccc gcacctagat
    25321 caccagtcaa tatcagattg tcacctggca ttgcggaatg accaattgcc tctgcgaatt
    25381 gttctgtcat tgttaatcct gatagttcga tcatttttca tcttctcccg ctcttctttc
    25441 tttttgccag tatatcgcgt aatgaagttt ttttaaagaa aaaatgaact agtcaagaac
    25501 agcagctgct gtttttggct agttcgcgtg tatttgttaa tcaatcaatg tttgtgctgc
    25561 tgtgataatc gataatttat atacatcttc ttcatttgct ccacgagaca aatcagaaac
    25621 cggtttgttc aagccttgta gaattggtcc gattgcttca aagttaccga aacgctgagc
    25681 aattttatag ccgatatttc ctgactgcaa ttctggaaat acaaagactg ttgcttgacc
    25741 agctacttct gaatctggcg ctttcagttg tgcaactgaa ggaacataag aagcatcaaa
    25801 ttgcaattcg ccgtcgatca tgtattgtgg cgctagtttt ttagcgattt tagttgcttc
    25861 aactactttg tctacttctg gcgatttcgc tgagcctttt gtcgagaagc tcatcattgc
    25921 tacttttggt tcgatatcga acaattctgc tgtttttgca gaatctacgg cgatttcagc
    25981 caattcttgc gcagttggat tcacgttgat cgcacaatcc gaaaagacat atttttcttg
    26041 atctcttcca cgtaccatga tcatcgctcc gcttgtccga ctgatgccag gctttgtttt
    26101 gatgatctga agtgctgggc gaacagtgtc gccagtcgaa tggatcgctc cagagaccat
    26161 tccgtctgca atccccatat acgtaagcat cgtaccaaag taattcacat cttttaaaat
    26221 cttttctgct tgttctttgg atgcctttcc atttctacgt tcaacaaaag tatccaccat
    26281 ttcatcccac ttatcgtaat tatcagggct gatgatcgta aagctagatg ttttgattcc
    26341 tctggcatgc gctgcatttc ttatttcttc ttcgttccct actaaaatgg gttccacaag
    26401 ttcttctgct ttcaaccggg cagctgcgcc taaaatacga gcatcagtag cttccgggaa
    26461 aacgattttg atcccacggc gaacaatttt aaatcttaaa ctatcaaaca attccacaag
    26521 atgacctcca gacgttttta tatgtattat catacactat tccagaaaaa aagaacagtt
    26581 tattttatct gtatcagtca aaaatgctgg aaaaattaag tttttcacat acaaagcgaa
    26641 ctgcactata acaggaaagc gctgttttat tacactgttt caggcagttg ccaatcaatc
    26701 ggttcttctc ctaacgcaag gagcgcatca ttcgttttcg agaagggttt ggaaccaaaa
    26761 aagccacgac ttgcagataa cggactggga tgtggagatg ttatgatgat atggcgactc
    26821 ttatcgatca ttttgatttt ttcttgtgcc ggttttcccc acaagataaa cacgattggt
    26881 ttttcccgtt catttaattt ttcaatgatc gtgtctgtta atctttccca acctttcccg
    26941 cgatgagaat acgcttgccc ttcccgaacg gtaagaacgg tgttgagtaa aaggactccc
    27001 tgtttagccc atgacactag atttccatgt tgaacaggtg cgattccaag atcatcataa
    27061 agctctttat agatatttct taacgatggc gggatcttta cgccaggttg cacagaaaaa
    27121 gataagccat gagcttgatt ttctccatga taaggatctt gtcctaaaat caccacttta
    27181 acttcttcat aaggagtcaa ttccaatgct tcatagatat gatacatatc tggatggatc
    27241 ttttgggacg catattcttt ttttaaaaat tctcttagat gcagataata atcttttgaa
    27301 aattctgagg acaaaacttc ttgccaactg ttatgaatga tttctttcaa aaactccacc
    27361 tcctaataaa aagaataact cacattttct tcaatcacaa gggacaatct tcaccctcag
    27421 cacttgatta atatggtaaa ctaggaacaa gaagcagaaa ggaacgataa tcattatgat
    27481 caaattaatt gcatcagata tggacggcac attattagat gcccatatga gtgtttctcc
    27541 tgaaaatgcc gaggcgatac gttttgcaaa tgatgccggt gttgagttca tggtcgctac
    27601 tggtcgaaac tatcaagaag ctcgcgcagc tttagatgag gtaggaatcg attgtgcgat
    27661 gatcactttg aacggggcac aggtatttga taaagaagga aattcattgt ttaccgtgtc
    27721 gattccaaat aatcaagcaa tctctgtttt agatattcta gatgctaacg gtatttacta
    27781 tgaagtagca accaacgatg gtctttattc tgaaaatcaa gcgaaacgaa ttgaaagttt
    27841 tgcttccatg gtggctaccc atttacctca ccttacgtat aaaatggcga ttgccatggc
    27901 ctcagctaag ctggagttat tgcacatcac atacgtggac agtattcgtg ctattttgga
    27961 agaaaagaat ctggaagttt taaaaattat ttgttttcat acagaagggc cagctgtatt
    28021 aggtccagtt ggaaagcaga tttctctttt aggcgatcta gccgttactt cctctggtca
    28081 aaacaatatc gaagtgaacc ataaaaacgc tcaaaaagga attgctgttg cccatgtcgc
    28141 tcacgagcgt gggatttctt tagaagaagt catgacgatt ggcgataact tcaatgatgt
    28201 cagcatgctg caagtagcag gagtcagttt cgcaatggga aatgcagaaa tcgaagtaaa
    28261 agattatgcc aaatatacaa cggacacaaa ccttgaatct ggcgtaggaa aagccatatt
    28321 gcgcgcgatt gcagaagact tgtagtttta tctagaaaag agggatatag atgtcagaat
    28381 ttttcattca ggaaaagcag cttggacgtg caactcggac aattgtcaaa aacgaaaaag
    28441 gacattccct gtttttgatg gttggtcgct ggggaacaaa gggcgatgca ttgtctcttt
    28501 atgccatgaa tggcgatctc gttgctcaca tcaagcagat aagcctgaca ttcggtacgc
    28561 gttttgagct gtataaagag ttcgaaaagg tgggaaccat gcagaaaata ttcaattggc
    28621 ccggtgactt ttattatatt cgtagacttc actggacagt ccaaggtgat atctataatc
    28681 atcaatatca aatccatcat ttcaatcagc aaatcatgaa aatggataaa gcaacacttt
    28741 tttcaggaga ttactatgta ttggatgtct caaatgatga agatgctcca gtttgtattt
    28801 gtattgctgc ggttttagat tactggctct accgtaaaga caaaagtcgc gataagcctg
    28861 tttcttaccg acttaactta agttaaaaag atgtacctga aatgcggaaa tacgcatttc
    28921 aggtacatct ttttagatat tcttttgcat atgatcataa agatgaccac tgatcatccg
    28981 ctgaccgaca actttgaagt catggcgttc gtataatttt ttagcctttg gatttttttc
    29041 atccacactt agaccgatga cggagcgatt agctttcttc gccaatttag gtaatgcttc
    29101 taataatcgc gaaccgatgc cttgtcctct aaaatcttct gatacactga tagagtccaa
    29161 ataccattcg ttgggaaacg tttctttatc aatgaacaaa cgtgtagacg catctagctg
    29221 atatttctct aagataggta ccagtggctg atcgatgatt gattcttctt catctgtata
    29281 accgtaagca actcctgcaa ctttaccgtc tatttcttct acgatacctc gtgaataact
    29341 gtaacgataa tttggatcag tgattgcttc ttctaaaatc tctaaagtct tttgttcccc
    29401 tgtctgcttc aaaaatggca gttccatatc ttttagaatc actaagatca atggggcgat
    29461 ttctggagca tcttcttttc tcgcaaaacg aatcatagtt tcctctcctt ttctccattc
    29521 taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa aaatcttcaa tcttttcagt
    29581 aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt cggcaataag ggcagacgac
    29641 tggcatttct ttatcattat gttcgactac attattgact tcttcgtatg caaatttttt
    29701 tccgcaatta gggcatttca ttttttccat gttatctcaa cttcctttca tctttactct
    29761 cttagcttac tcttatattg taaaaaagag aagcaatatc cttcttctat gcagatatcc
    29821 atacctgacg gtcctgttct ccatctatta atcggacttt ataataagga cgttcatctt
    29881 tgactagaag tgtccactct ttcacttttg cttttttccc gtagtagctg actgcttcct
    29941 tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc tatatcaaaa agatgttctg
    30001 cggccttttc cgtgtcattt cgtacggttg ttttcccagt ggatggattg actgctatga
    30061 cttgtccttc acaaaaaaac tggtattcat atgttgcatt cgtaggattc tccaaatctt
    30121 tttcaactgc aaaatgaata gcagatatgt tatgttcttt tgtttcgttt tttacgattg
    30181 ctgctgcttt tttcaatgag atccgatatt cctggtcact aatatcatcc atgtgaaaag
    30241 cggcattgag cagtcccgtc ataccggtaa tccctattac gatgagggca gcaacaccaa
    30301 acatcatttt tttcattctt cattctcctg tttctttttt ttaaagaata tcaagaaatg
    30361 aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa ttttgttatc tttattcgtg
    30421 cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc attttttttc ctgtaaaggt
    30481 atcttctcta atcggttctg attcgatccc taaggactgc aattctgcaa ttgtttcttc
    30541 aatatcgtct actttgaaag caagatgacg caagccacaa gcttctggat agtttggtcg
    30601 ttgtggcgca ttagcaattc caaaaatctc caattcagca cttcctagtt tcagatctaa
    30661 tttatagtca tctcgttctt tgcggtaatt ttctctgatt acttcaaatc ctaatttatt
    30721 aacataaaaa tcacgtgatt tctgataatc tgaaacaatg atggctatat gatggatcgt
    30781 tgtaaggttc atatgagcac tcctattctt cgtttaagct cggatctata ttaatctcca
    30841 gttccattgt aaatgtgtca gattgaaaaa aatagctgtt tttccttttt ataagaaaaa
    30901 aggcaaaaaa aacagaaagt aggtcctcca agacactctt cctgttccca atataataaa
    30961 tgaaagtaag aaattatatt ttcaagaaaa caaacagaaa attatcataa cacaaattat
    31021 ttataattga aagactagtt ttttttcaag aaaagcatac aatagattgt gttagtaaat
    31081 acagaacaca ctacatttag tgtcttaagg agtcctcata catgaagtca tattttcagt
    31141 ttttagctca tcagctaaaa ggctggccgc aacaaaatta ttatcttttc tttttcagtc
    31201 ttggctgcca ggtcatgact cttgtcaata gtcccatcac ttggctgtcc gttattacat
    31261 ttatcggtac cacgcttggt gtattgtgta ttttatccat caatgcggca aaatcagtca
    31321 acggtatttt aggtattttg tctgcgattt gttttattat cgttgggttt tcagcaaaga
    31381 actatttaag tatcggtgaa cagattgctt atatgatcac attggatatc cccgtattgt
    31441 taagtaaaga atggaatcat aatatggctt caaaaatccg aaaattaact ggaaaaagct
    31501 ggtttatcgc aatcatcagt actttccttg tctatcttgt ttcaggattc ctgattggtc
    31561 gttatacaga tgatcctcgg ccgtgggtag atgccattgc tttttctatt agcttgactg
    31621 ctggtatcat cagtttcttg cgttacaaca accaatatta ctggtggtta gcttcagggc
    31681 ttgctcaaat ggtcctttgg tatatttcat ttacgcatgg atctgcaagt ttggcgatgt
    31741 tcgtgaacag ctctgtttac ttgctgaatg atgtccttgc atttaccgtt tcaccttggt
    31801 ataaccaaaa agaacgtgaa cgtatgcaaa aaatggaaga agaatactac aacgcacaac
    31861 aacacgcata aaaaaactga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
    31921 aaacacgtat aaacggcgga atcagaagca actccttcgg aaataagctg aaattcacaa
    31981 aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc
    32041 ccagcctcta ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc
    32101 agtgattctt ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga
    32161 taatgttttc ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc
    32221 actactagtt gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata
    32281 gcttctcgtt ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg
    32341 tcgaagaacc cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt
    32401 tttggttgga tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt
    32461 gttcgttgac gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga
    32521 tgtactggca cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag
    32581 ttgatcaaga attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct
    32641 ttttttatct gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca
    32701 ttttccaatg cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg
    32761 agccgctgag ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac
    32821 aagacaaaac agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc
    32881 cataataatt cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc
    32941 actgtcggag agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt
    33001 ccggtttttg gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac
    33061 aatttttgct ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc
    33121 caatctgttg cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg
    33181 tgcttatcga ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga
    33241 ctgcttgtgt attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac
    33301 cattcttcaa agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa
    33361 aagctggcta ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt
    33421 gttccggcat ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc
    33481 aaagaagaag caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact
    33541 tgatcgggtg agaatggcac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg
    33601 atcaaatgaa gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag
    33661 atgtacgctg gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag
    33721 gtaaagtaag cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca
    33781 ttatcaggga ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac
    33841 cgcccacctg cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct
    33901 ttgactactt gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca
    33961 ccgctgattt catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca
    34021 ccaaatggat caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt
    34081 taacatatgg ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa
    34141 tagtttaaat aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac
    34201 tacaatgtta gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta
    34261 aatagaatca tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt
    34321 ttgatcactc cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta
    34381 gcagccatcg gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt
    34441 aacgctggat tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg
    34501 gtgatggccg caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa
    34561 atgatgatgc cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc
    34621 actgtcccaa ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac
    34681 atgcttaacg gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt
    34741 cgattatctc gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg
    34801 ccgactaaag cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc
    34861 aaagctggaa taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt
    34921 gatggaagcc ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca
    34981 aagatcaagc caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct
    35041 aagaccgtcc agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt
    35101 cttactccag tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta
    35161 tcgcctaata aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata
    35221 atatttcttc gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc
    35281 cgtgatagcc actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag
    35341 atctttcagt gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc
    35401 aaagaaccag cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt
    35461 aggatggact tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg
    35521 acgaacaaat ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa
    35581 gatcccataa atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga
    35641 agccgctgat tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat
    35701 aaaaatcgcc atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg
    35761 aatgccaaga ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga
    35821 gtctaattta aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt
    35881 ttccatatat tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata
    35941 aaaaatatag ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt
    36001 ttattatata attcacctta cttattgtaa gataaagctt tttttaattt attgcctgct
    36061 aatatataaa aataaggtct agttactgtt tttttaaatg attaataaat aacaactcct
    36121 ttctatgaaa ttgcaaagca ttcatcttct tttctgctaa cgatatatcc gtcaatgctt
    36181 gttacacttt actaaaaagt atgagattgc cgatatctgg ataggtgtgc tatactacag
    36241 aaaaagatat acgatctgcc accgggtagt aaaaaagagg atgatcattc cattaggtct
    36301 ttgaagggat gattctcctc tttttgtgta ttttttatat tgaaggagga acgtatgtat
    36361 ggcatggatg gaattaatca tagcaggaat tttggaagta ttctggtcaa cgatgatgaa
    36421 atggagtgat ggtttttcta aaatcaatta ttctttttac acagtgattg gaatgattgc
    36481 tagtttttac tttttgtcaa aggcaatcaa aagtttgcct atgagtctct cttatcctat
    36541 ttggacaggg attggcgcag ttggttcggt cattattggt gtggtcttgt ttcatgataa
    36601 gctgaatctt tccacttggt tctttgtcgg gttgctgctc atcagcatca ttggcatcaa
    36661 aatcactagc ggtcactaga tacttagaca agcagacaag gactgatcag aaaaagcggg
    36721 gctttttatt tgatattcct ctttctatga aaaaacaaga aaataagact aatgacctag
    36781 cgacttctct actcatcagg tgtatgcttg tactatctat agaaaaggag tgaatatttt
    36841 tgaaaagtct ttcggtccaa gaattatcca agaactttgg tcacaaacaa gccctcagcg
    36901 atgtcagttt tagcttatct cctggcaaaa tcgttggatt agtaggtcct aatggcgctg
    36961 gaaaaacaac gatcatgaaa gctattttag gactgatcca ttactcagaa ggaacgatcc
    37021 gtattggtga aaatgagatt tctccctcta gtcataaagg attagaacaa gtaggagcat
    37081 tgatcgaata tccgggtatc tatccattct taacaggtta tgatcatctg aagttattct
    37141 ctgaaacaaa cgatgtatct gctatcgaca ctatcgtgaa acagctcaag atggaaaaat
    37201 acattcacaa aaaagcgaaa tcctactcgc tgggaatgaa acaaaaattg ggaattgctc
    37261 ttgccttact gaaccatccg tctatcgtta ttttagatga gccgatgaac ggtttggatc
    37321 cacaagcaac aagagatgtg cgaaaaatca ttaccgactt ggctactcag ggcacctctt
    37381 tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat ggtcgatgag ctgatcctga
    37441 tcaatcaagg aaaaatcatc aaacaatgta caatggctga attgaaccaa acagcgcaag
    37501 atttcatcgt tatctctacg acaaatgatc aagctgctcg aacgatcttg acagacgctg
    37561 gatatccctt ggaagatcct gataaaatca agcttctaaa aaaaaacgaa gagttattag
    37621 gaactgttat ccagttattg tctgcagaaa atgtccatgt gacagatgtc cagcatctga
    37681 cgaatgattt ggaatcttcc ctattggaaa ttctggattc agtaaagggg gaagaagaaa
    37741 aatgacgttg cttataaaac aagaattatt taaattgatc aagaaaaagt caactgctgt
    37801 tttatctgtt ctattagttg ctttgctcat aggaacagca cttctagcca aaaaatacac
    37861 gacgatcatt gatccagttg aaatgactgc tcagcttttc agtgccacat cttggattgt
    37921 ctttatcatg attgctgctg caagtacgat catttcaatg gaatcacaat atggtacatt
    37981 gaaaaatctc ctttatcgaa agtattctcg aggagaaata ttagtaagta aatggatcac
    38041 attagttatt tattctattt atttatacct tttagctatt attgtcaccg tgctgatgaa
    38101 actgatcctt ttcccttcga tttcctttac ggagaagatt tctactggtc agacactgat
    38161 ccaaagctta ttcacttata ctttgggtag ttatatcggt ttatggctaa ttttaagttt
    38221 agtgttgatg attgcttgtt ttatcaatag ttccggtgcc tccatctcag cagggatcgt
    38281 cttttatttt gcttcatcaa tcatatctgg tatcctcatc gcactcattc aaaaatggga
    38341 gtggatcaaa tggaatccaa tcagtatgct gaatctgcaa aaccaaattg gcaacgaaga
    38401 gattatgaag caattgactc atctttctac taatcaaatg ctgtttggca atttagctta
    38461 cattgctttg ttcttagcat tgggatacct cgtattcaag cgaaaaaatg tatgatggtt
    38521 cattaacaaa gaagttgtga cagaagtggc cagtttcaag aaatttcggc ttatttttag
    38581 aagaagctac ttcttgaaca ccatttattc aaaaaaagaa gcgatgacac gaatgttgtc
    38641 accgcttctt ttttgaataa accacaggct tcacagcctc tctttatact tctatacgtg
    38701 aagcatccca gtggcaaata aaaccattgc gattactccg accatcgtca caccgataag
    38761 taaaatctta tctaagtgga tatgttgtac aggttcgttt tgttctttac gtgctttgag
    38821 gtaaaaataa atacctggta agaaaagaac aagaacaagc atcacttcat taaaggctac
    38881 aactagcatg gcccatactt gaaaagcact tgctaaaaac cctactaaaa attggaccca
    38941 ctctttccgc tgataagaaa gcttcatttg gtacagccct aagaaaagcc acgtgaagaa
    39001 aatcgctgct gtaccaagtg tataagcaaa gttatatgca ctgtcagtga ataataatga
    39061 aagtaaaaag acattcgtac ataacgtcgt caatacgata gcataagttg gtgccccatt
    39121 tttgttgagt tttccccatg ctttaggcaa aagtccgtcc tcagccatat tccccattgt
    39181 ttcaatcggc aagattgtcc atgagagcca aaccccaact aaagaaatga tcaaaccgcc
    39241 gttgatcacg accgcccccc atttaccaac catcatttca aatacatatc ccatagcagg
    39301 ttgcgctttg atcgtttgca gctgattcat cgtcattgtg ccatatggaa gcatggaaac
    39361 caagacgtaa ataatgatca aaccgaatat tcctagaatc gttgctttcc caacatccga
    39421 ttgttttttt gctcgtgaac ctacgacaga agctccttca ataccaacga atacccagag
    39481 catcaccatg atgcaactca atacttgact agaaaacggg atcggctgcc cattcgcatt
    39541 caatatgata tcgctagaaa agttattaat gaattgttgg atcgaaaagg tgctgatagc
    39601 tactacaata aataaaaata atggcactaa tttacaaaat gtgacgatag cattgacaaa
    39661 gctagcattt tcaactccac gattcactag ccatgcatat ccccataaga agatcgtact
    39721 catcacgatc gatgccacat tttgaccacc ttcgaatacg gggaagaaat aacctaatgc
    39781 actcatcaac aatgttgcga acgccacgtt tcctaaccag catgtgagcc agtaacccca
    39841 accgctgata aaaccagcga aacggccaaa accttcttct gcgtaactaa agatcccgcc
    39901 ttttaaatct ggacgttttt tattgatatt attgatcgat aaggagagca tcaacattgc
    39961 tgttcctacg agtacccagc tgaagatagc tgggccacca gccgatccgg agatgtccgt
    40021 cataatacca aatacgccac ctccaatcgc tgaaccaatc actaaggcag caagtggaat
    40081 caaaccaatt ccttttttct gttgctctgt atccatgtaa gcatcctcca aagtaaaacc
    40141 gcttttatca aaaagtataa caaacctttt ttatttttac ttaattaata aaaactttaa
    40201 tagaaatata attctaccga cttttttctc attatacaag caaagcgcct agtccctttc
    40261 caaattacac caccaaaaaa acttaaaaag atgctatact aaagggaaag aattggggga
    40321 tgatccatga acattttata tatatcgatt tcaggaaata cgcgtgcttt tgccaaacat
    40381 ctagcagaat atgctgaaaa aatgcatgca gaagatcctg tcaatcctga agttacttta
    40441 aaagaaatcc atgaaaatag tgattttacg aaagaaacag aaccattttt tactttcgtt
    40501 cctacttatt tagacggcgg gaatgggtta gacaacggtg acacagaaat cttgaccgaa
    40561 acgatgcgcg aatatctgga atacgaagac aatcataaac tttgccttgg tgtagtcggt
    40621 agcggtaata aaaacttcaa caatcaatat tgtctgacag ccaaacaata cgcacaaaga
    40681 ttcggctttc ctttcttggc agattatgaa ttacggggaa caccatctga tgtgacacgc
    40741 gtttatcaaa ttttggcaga aaaaaataaa taacacaaaa gaagaagctg aaaaataaat
    40801 ttcggcttct tctttttatg ttctctattg tttctattct tattaagctt tgacatccat
    40861 cacaggtgct tttgctttcg ctttacctgt tttttccaag acaaagtttt ttacacgatc
    40921 catattggtt accaagacga tcatggaggt ttctttgtct gctgctttga ttgcttctaa
    40981 gtcagcttct gctacaagcg tatcaggtgt cacttgctgt ccttctgtta ctttcacatc
    41041 gaatggcgta ccgcctaagt caactgtgtt gatgcccatg tgaagcaaga tttctaagcc
    41101 atttggtgtc gtaatgccga tagcatgttt tgttgggaaa acagaactta ttactccagt
    41161 aaccggggaa tagatatgtc cgttttctgg ttctacaaag taaccttcac ccatcatttt
    41221 ttgtgcaaaa actgggtcac ttgctttatc taaagaaaca actgttccgt cagcaactga
    41281 tgataagata gtcacacttt cagctgaagc ttgaggtact gctttttcag gtacttcatt
    41341 tactgtggct gtttcggtcg ctagttcttc tttttctaaa cggtctttag gaataccgaa
    41401 gaagaatgta gcaacgaagc caccagcgta agcagccaat aaaccaagga tatagcctaa
    41461 ccatttacca tcagagatca atggaatcaa agcggctcct gaaggtccaa tagcgattgc
    41521 tccgacattt ccgatcattc cgatcactgc cccaccgata cctccaccga tacaagcagt
    41581 aataaatgga cgtcctagag gtaatgttac tccgtaaatc aatggttcgc cgattcctaa
    41641 gattccgact ggtaaagccc ctttgatcat ttctgttaat tctttgtctt ttttgcagcg
    41701 gatccataat gccaatgctg cacccacttg tcttgctccg gccatcgcaa ggattggaag
    41761 caataaggta cgaccagttt catcaatcat ttgtacatgg attggcgtca agatctgatg
    41821 tagtccgaac atgaccattg gcaagaataa tgtacctaga acgaagccgg caaatgctcc
    41881 tccaacacca agtacccagt tgatcccacc gaccatgccg tcagaaatga accctgctaa
    41941 aggcatgatg aagaaaattt cgatcagacc gatggcaatc agggaaagag ttggcgtaac
    42001 gatgatatct accgcatcag gtactacttt atgcagtttt ttctcaacga ttgacaataa
    42061 ccaaactgcg aagataacac ctaagatccc gccttgtcca gttgcaagag cttcaccggt
    42121 aaacaagttt ttcagtggtg cttctggatt catccctgtc agcatcacaa cagccccgat
    42181 aactccacca agcgttggcg ttgcaccaaa aacttgtgca gcattgatac ctgtatagat
    42241 aactagatag ctaaacatcc cgtttttcaa gatgttcatc acatcgatat attgttgcca
    42301 tgttcctgcg tctagtgttc cagctgtgac taagtttgct aaaactgcgg caatccctgc
    42361 cacgatccca gtgccgacaa atgcagggat cattggtaca aagatattag agatgtcttt
    42421 taataccgat ttgattttag agggtttttg tttcgatttt tgtgccgctt tcatttcttg
    42481 tgcccgttcg tttacacggt cttttccaga tgcagaagaa cttgctgtag ttgcactagc
    42541 agaaggaagt ttttctccta atttcacgcc tgctaagtca accatttcct gcgctacttt
    42601 gttcactttc ccaggtccga tgatgacttg gagctgttca tcctctacta cgccaagtac
    42661 tcctggaatc gcttttaatt ccgagacatt tacaagatca tcattgatga ccttcatacg
    42721 tacacgagtc atgcagtgga caatgctttc aacattgccc ataccgcctg ctccttgata
    42781 aatttgtttt gccagttctt ccaaagtcaa ttcgtttttt gccatggtct tttccccctc
    42841 ataaggttcc ttttacaaaa ccgtttgctt cagctaattt tgcttgagct gcctctttat
    42901 cactatttgt caaaatcatg acgatagcca gtttgacatt ttgatctgct tcctcaaagt
    42961 acttttcagc tgtttcgtag tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt
    43021 caattagttt ttcatttgtt ggtttgacgt cgaccatcaa gttattgaat actttaccga
    43081 taccgatcat actgatagta gaaatcatat tcaaaatcag tttttgtgct gttcctgact
    43141 tcaatcgagt agagcctgtc aaaaattctg gtcctgcatc gacttcgatt gggaaatccg
    43201 catgctcgct gatttctgct tgtttattgc aagaaatcgt tcctgtaaca gcaccaatcg
    43261 attttgcgta atctaatcca ccaatcacat atggtgtacg accgctagct gcgataccaa
    43321 taactatatc attttttgtt aaattcagct tgattagatc ttcttgccct aattcataag
    43381 aatcttcggc tccttcgacg gctacagtca ttgcttcttc tccaccagca attaggccta
    43441 tgaccatttc cggttctaca ccgaatgtcg gtacacattc agcagcatct aatacaccaa
    43501 gtctaccact cgttccagct cccatataaa tcaagcgacc acctttttta aaggattcaa
    43561 tcgtgttggc gatcactggc tcgatacttg gcagttcttt ttcaacagca gcggccactt
    43621 tctgatcttc tttgttcatg agttggactg cttgagaaat actcatttca tctaatccaa
    43681 aagtttcttg gtttctgcgt tccgttgtta ggttctctaa attgattttg ctcattttta
    43741 tcttccttct tttcttataa attgatagtt cataccagct ttgatttggt tgatcagcgg
    43801 tagttcttct ttgatgactt tggctactac attcactttt tcatccgctg gcaaatcata
    43861 tttagttaat tggatctcac ccatatagcg caaatacttt tcgttatcta aagtaataga
    43921 tcctttcata cgcttagctg tatttcttac tgggattggc ggaatctctc gaaaacgcgc
    43981 atctgcgctt cgtatgacat ctcttgcatc atcttgccga ttcgtatgtt ttcctaatat
    44041 atattcgtaa aaaacttcat ctacaggttc cgtatggagc aagactgtct gttctttttg
    44101 gaaagaagca aattgctcct gaacgtgttc tgacagacct gcatctccga tataaacaat
    44161 atccgtgtta cttgctaata gatcaagagc acctgccaga ggatggatcc ctcgatgttc
    44221 ttccaaagtt ggtaatcctt gatacagtgg acctctcaac tctgtatctc ctggtacaaa
    44281 gccttgtaca aggaatcctt gagttttgag ccattgattt ttttcctgat accattcttt
    44341 gtccaaccct gtttcaagac gtggataata attatgccaa gcttcaatct gtgagaaatc
    44401 agccgctgct tctttcaact catcgatatc attaggcgtg atcgtgctgg cattcaaact
    44461 gattttcaat tgatgtgacc agtcggcgat ctgctgatta gaaatatgat aatccattct
    44521 taaccctgtt acaccaattg ctttcaaggg tgttagatct tctaatgaaa agccagctcg
    44581 tttcaatgcc tctcctgaaa tatcgaccat caactctagt ttattctttt tcgcttcgct
    44641 tcctagttcg atcaatcgat tttgataagc cgaaacatca tcttccggta tatgcatgga
    44701 agtgaaaata ccgacaaagc cattggatgc catttggcgg atatactgtt tcttctcttc
    44761 tgacaaagaa tcattcataa atattgaaaa gccaaacata ttatccctct ttttctgtat
    44821 ccgtcttcat ttctatgact agagtatata tctaaagaaa caaaatttca atacttttgt
    44881 taaaataatt aaattttatt tctaaaaatt ggatttataa tgccaaaaga attgacacag
    44941 ccacaaaaaa ggcactgcta agcaaaagca gtgccttgaa acattttatt taaataacac
    45001 aaaagtctaa ttactcataa gaaattaatt ctgtattgtc taccatgaat gtaaagaacc
    45061 cttcattagg cattggatga aaataagcat tgatcactct tatcaaggca ttatgacaga
    45121 ctaacaagta gacattttct tcgtcttcca tgcattcttt caataaagga acgatccgtg
    45181 cgtacacatc taagaccgac tctccatttg ggaaacgtac agcaaacgct aaacgcgctt
    45241 tttgaaagtt ttcatctttg cttggcagtc cgtcataatc cccaaaatcc atttctacca
    45301 atcgctcatc agcggtcatc ggcaaattga gacgatcaac tactgcttgt gctgtatctc
    45361 ttgctcgttg aagaggggaa tggatgattt tcgtgattgg aacaggcaaa tctactacct
    45421 tttctgctag tgcctgtgcc tgcatctgtc ctttttcagt cagcggcaca tctgctcgcc
    45481 cgcaaactaa tccttgtgcg ttccaagttg tttctccgtg tctcgttaca taaagcatgt
    45541 gattctctcc ttcgttgaca aagttctcct taaccatacc ttgttgtctt tgagattaca
    45601 aggtttagat tttctcaaga gaaataatct cagtagatac tttatctaga aaggtttgat
    45661 catgttccac tagtaacata gcaggtttca cgttcaaaat cagttgttct aattgttctt
    45721 gattgaagac atccaaataa ttcaatggtt catcccatat atagagctca gctggttggg
    45781 ataaagattt agccaattcc acttttttcc gttgtcccat actcatctgt tcgattttat
    45841 tatgaaaaac atctctttcc atcccaagct ttcggagatt attcaaaaat gcttgatagt
    45901 ctactcggtt tttcttcgcg aattccgcca atgtccctcg attgtcttca taattttggc
    45961 gtacgtaact aatggtcaaa tgtttgggct gggatttttc tcctacaacc ttgccgttga
    46021 atgtccccag acgataatgg ataatggatg actttcccgc gccgttagga cctgaaattg
    46081 ccatccgctg atgaggttcg atcgtaaaat gaactggctc gaataacaga ttttcatacc
    46141 ctaattgaag attttctacg cttaacagcc gtttatgatg agacacttgg ctattcatcg
    46201 tcaaagcatc gatatactcg atatctttta ataatttttc tttctcactg atctgggttt
    46261 ccatccgatt cacgattgct ttagatcgtt tcatcgtccg tgcagcatca gcgcctactg
    46321 ctcctttatt cactcgccta gattccgtat cgatgaatcc gacttttttc tttgttttgt
    46381 ccccttctcg ggaacgagac cattctgctt tttcagctgc tgttttcttt agcctgctga
    46441 cttctttttt caatttttca tttttagcta tttcaaattc atcacgaagt tttttctgtt
    46501 cttcatagat agagaaattc ccttgataaa gttccagttg gctcttttcg attgctaaaa
    46561 catggtccac tacttcatcg ataaatcccc gatcatgact caccacgatg aatccttgtt
    46621 ttttcttttt caagtaagcc gctacttgtt ttctgccaga gatatccaaa tgattcgttg
    46681 gttcatcgat taacgggaaa tgagtgtcat ccacaaataa gagtgccagt aggacttttg
    46741 tcttctctcc tcctgatagt gtgctgaatt ctctccataa gatttcagga tctgtttgca
    46801 tcagttggag ctctctttca atttcccata tctcaaaatc cataatctca tttaacacat
    46861 aataggtcaa acgctcttta tcttttgttt gctgcgggaa atagacaaac gcttgctgat
    46921 ggatcacttg cccttgataa gggagtttgt tttgcaggat attcagtaaa gttgtctttc
    46981 ctcgaccgtt tcgtccgata agtcctagtt tccattgtgt gtcaaagttt agattggctt
    47041 gttcaaataa taatgtgcct tggctgtcat agccgaatgt cagatttttt atttcgattt
    47101 tagacattat tccatcacct cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag
    47161 aggatgcaag ggttggggca cacaaaaaga cccatacatc gaaagaacca agccaatccc
    47221 gaaaatctgc acactgctcc cttgtctcca caaggtacag tctgaatgca gctttcatca
    47281 atcgacttaa tttttcaatg cacggatccc tcactttgtc ctatatttga aattacgata
    47341 gcataaagaa attgtttttg tcaactactt cgggagaatg ttgaaaaatc tgctgagtgt
    47401 ctgtcctaag atttctgttt tttctaatgt ttgcctgtgt tccattgctt cctctaaatc
    47461 gattggtcct aattgaatgc ttaagacaat ggggagaaaa tcaaagagtc cttccagctc
    47521 tcggtctcta cttccgcaaa tagcgattat tggttttcga tatttttttg caagcactgc
    47581 tacgccatat ggaacttttc cgtggattgt ttgctgatca atccgccctt ctcctgtaat
    47641 gatgacatcc gcttgttcga tctgtttttc taatccagtg atttggctga ccaactcaaa
    47701 accagagacc atcttcgctc ctaacaaaaa aagggctccg ccaataccgc ctgctgctcc
    47761 gcttcctggg atgtcatcta aatccacctc ccaaacatct tttatctgct gagcaatctt
    47821 ttcagcttgt ttatccaaga aagctaacgt cgtatccgtt cctccttttt gtcttccaaa
    47881 ttgccaagca gctccttgtt gacctgtata tgggttggtc acgtctgcag cgatgatcac
    47941 ttttacttta gaaacaagaa cgttccttcg atccaaatga agttttctag tagataacaa
    48001 aggatttcct tctgtgtacc cttctaaaga agcacccaaa gattggagta atcctagacc
    48061 accatcgcta catccgcttc ctcctaaagt gacaatgatt tgttccactt cttgaagcag
    48121 tgtatctttg ataagctctc ccaaggcata actagaagca agtcggatgg tttgatccgt
    48181 tggtttatca agtaaatcta atcctaatac ttgtgcagat tcaatgactg cggtattttt
    48241 tccttgccag ttcgtcaata aataactgat ttttcgctga cgaaataaga gatccatccc
    48301 atcaatcgta cgccaggttc ccccgagcgc gtaatagatt gcttctgcag tattttctcc
    48361 gccatctgca atcggcacat tgatttttct tcctttacgg tcttcccaac catttagaac
    48421 tgcctgattt aattcaaatg aagaagcact tcctttaaat gaatcgattg ccgccaaaat
    48481 atccatctat atccctcact ttcttcttaa gtaagacact ttttttcact tttttcaaac
    48541 aaaacaatgc tattttcttc tgtttttcta tatactaaaa gggaactcat tgaataaagg
    48601 agagaaaaaa catgaaaatc gcaatagctg gagccggagc aatgggcagc cgaatcggat
    48661 taatgttgca tcaaagcgga aatgaggtcg tcttgatcga tcgctggcct gctcacattg
    48721 aagccattcg tacaaatgga ttgattgctg actttaatgg caaagaagta gttgcaaaac
    48781 tgccaatcta ttcaccagaa gaaatcatcg aaagcaacaa acacgtggat ttgatcgtag
    48841 ccttgacaaa agcgaatcaa ttagatgata tgtttcgttc gatccaatcc attattactg
    48901 agaatactta tgttttgtgt ttactgaatg gattaggtca tgaagatgta ctggaaaaat
    48961 atgtaccaaa gaaaaatatc ttatttggga tcacgatgtg gacagctggt ctagctggtc
    49021 ccggaaaagt cacattatta ggtgatggag aaatcgaact agagaacttg gaaccagaag
    49081 gcgaagcatt tacaaaaaaa gttgtagaag tctttcaaga agcaaaattg aatccaatct
    49141 atagtcataa tgttcgttat tccatttggc gaaaagcttg tgtaaacgga acattaaatg
    49201 gattatgcac gatattggat tgtaatattg ctgaattagg tgcgcaaaaa gcggcagagg
    49261 gcatggttcg aacgattgta tctgagtttg cttctatcgc agcaaaagaa ggaatcacat
    49321 tagatcaaga agaagtgtac caacatattg cttctactta tgatcctgac aacatcgggt
    49381 tgcattaccc atctatgtac caagacctga tcaaaaacca tcgtttgact gaaatcgact
    49441 acatcaatgg tgccgtttgg cgaaaaggac aaaaatatga tattgctaca ccatattgtg
    49501 cctttttgac ccaattagtc catgcaaaag aaggaatcct tggcgcagaa taaaaggctc
    49561 tctgatgaga atcagctgga tcagcagctg gttctcattt ttttaccccc atgaaagttc
    49621 aaaaagaaaa acacaattct catgttgcca taagaattgc gcttgtttat tttcatctat
    49681 gattggttgg cacttttatt tatttttaac agaggaaaga tcgtattcat attgttcata
    49741 aatagcggag tcagtagtga aaaaacgttt tgcccaattt tctgcatcaa aaaattccaa
    49801 cagttctgat gggattggtt gaaatggcgt atgcaaaaat tccggtaatt catctaaatc
    49861 acgttcttga agcaaaaaaa tattttcaga atgataaaac tgataatgag ctatggcttg
    49921 attatctgtc actagtttct tttgaaaata catagattca aacgttcgca aagtcactcc
    49981 ttgctgccct tcccgcaaca gttctagaat ccccctcgat tccccggttt ttttcagata
    50041 ttcttcataa gtaaggtatc caggttgttc atttcctcga agcggaagaa taaagtaatt
    50101 ggtcgtaaca cctctttttg taaattcctg cttatatttt tctgcccgtt cttttctgcc
    50161 gttatttgtt gcaccaaaaa atagatccgt ctttggttcg ctagaagaaa aatccatatg
    50221 tttcgaataa aaggaactat tatgttttag tccaagttct ttcgcttcta aaaaatcaaa
    50281 atgataaatc tcatcgattg ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc
    50341 tgctttaaag tggttccaaa aaaataacac gatttttgca ttggtaaatg gacgcacgcg
    50401 tcgagccatc tccatatcaa atccataatc taaaaaaacg atcagctgat aatttgccaa
    50461 ctgttgtcga gctgatttaa tgaattccga ctgtagtaaa tcttttccgg accaaacatc
    50521 taaagagcgg atggaaggaa aaaaataaag tgatttattt ttcgcaatta gcaatacgtc
    50581 gtttttgttc atctgttccg cctcactttc tctgtcactt tttcctatga tagcctgttt
    50641 aaatagtaaa aagccagtaa aacgcttcct attattttta taaaaattta acatttctat
    50701 gaaaatacga tgaaataaat cattcttcca ttaaaatatg cccaaaagtt ttctgaatag
    50761 ttgtaattat ctgaaaattt gttatcatta gt
//
LOCUS       DACDCJ010000011        46678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDCJ010000011 DACDCJ010000000
VERSION     DACDCJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.011"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="IV038_000638"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="IV038_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP8340881.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(1122..2498)
                     /gene="trmFO"
                     /locus_tag="IV038_000639"
     CDS             complement(1122..2498)
                     /gene="trmFO"
                     /locus_tag="IV038_000639"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP8340882.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(2467..4545)
                     /gene="topA"
                     /locus_tag="IV038_000640"
     CDS             complement(2467..4545)
                     /gene="topA"
                     /locus_tag="IV038_000640"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP8340883.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(4667..5524)
                     /gene="dprA"
                     /locus_tag="IV038_000641"
     CDS             complement(4667..5524)
                     /gene="dprA"
                     /locus_tag="IV038_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP8340884.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(5580..6347)
                     /locus_tag="IV038_000642"
     CDS             complement(5580..6347)
                     /locus_tag="IV038_000642"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP8340885.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDVRSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(6340..7200)
                     /gene="ylqF"
                     /locus_tag="IV038_000643"
     CDS             complement(6340..7200)
                     /gene="ylqF"
                     /locus_tag="IV038_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP8340886.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            7460..7858
                     /locus_tag="IV038_000644"
     CDS             7460..7858
                     /locus_tag="IV038_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8340887.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            8095..8637
                     /locus_tag="IV038_000645"
     CDS             8095..8637
                     /locus_tag="IV038_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8340888.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(8720..9271)
                     /gene="lepB"
                     /locus_tag="IV038_000646"
     CDS             complement(8720..9271)
                     /gene="lepB"
                     /locus_tag="IV038_000646"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8340889.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(9423..9650)
                     /locus_tag="IV038_000647"
     CDS             complement(9423..9650)
                     /locus_tag="IV038_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP8340890.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(9647..10165)
                     /gene="msrA"
                     /locus_tag="IV038_000648"
     CDS             complement(9647..10165)
                     /gene="msrA"
                     /locus_tag="IV038_000648"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8340891.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(10185..10898)
                     /locus_tag="IV038_000649"
     CDS             complement(10185..10898)
                     /locus_tag="IV038_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP8340892.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(10901..11797)
                     /locus_tag="IV038_000650"
     CDS             complement(10901..11797)
                     /locus_tag="IV038_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8340893.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(11955..12797)
                     /locus_tag="IV038_000651"
     CDS             complement(11955..12797)
                     /locus_tag="IV038_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8340894.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            12929..13582
                     /locus_tag="IV038_000652"
     CDS             12929..13582
                     /locus_tag="IV038_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8340895.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(13645..14172)
                     /locus_tag="IV038_000653"
     CDS             complement(13645..14172)
                     /locus_tag="IV038_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8340896.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(14192..15139)
                     /locus_tag="IV038_000654"
     CDS             complement(14192..15139)
                     /locus_tag="IV038_000654"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8340897.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(15151..17037)
                     /locus_tag="IV038_000655"
     CDS             complement(15151..17037)
                     /locus_tag="IV038_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8340898.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(17197..19275)
                     /locus_tag="IV038_000656"
     CDS             complement(17197..19275)
                     /locus_tag="IV038_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP8340899.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(19288..19563)
                     /locus_tag="IV038_000657"
     CDS             complement(19288..19563)
                     /locus_tag="IV038_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340900.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(19925..20380)
                     /locus_tag="IV038_000658"
     CDS             complement(19925..20380)
                     /locus_tag="IV038_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340901.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(20534..21742)
                     /locus_tag="IV038_000659"
     CDS             complement(20534..21742)
                     /locus_tag="IV038_000659"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP8340902.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(21854..22186)
                     /locus_tag="IV038_000660"
     CDS             complement(21854..22186)
                     /locus_tag="IV038_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8340903.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            22334..23209
                     /locus_tag="IV038_000661"
     CDS             22334..23209
                     /locus_tag="IV038_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8340904.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(23291..23872)
                     /locus_tag="IV038_000662"
     CDS             complement(23291..23872)
                     /locus_tag="IV038_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP8340905.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(23878..25137)
                     /locus_tag="IV038_000663"
     CDS             complement(23878..25137)
                     /locus_tag="IV038_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8340906.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(25139..26185)
                     /locus_tag="IV038_000664"
     CDS             complement(25139..26185)
                     /locus_tag="IV038_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340907.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(26420..26695)
                     /locus_tag="IV038_000665"
     CDS             complement(26420..26695)
                     /locus_tag="IV038_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP8340908.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(26938..28248)
                     /locus_tag="IV038_000666"
     CDS             complement(26938..28248)
                     /locus_tag="IV038_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP8340909.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(28430..29659)
                     /gene="rpsA"
                     /locus_tag="IV038_000667"
     CDS             complement(28430..29659)
                     /gene="rpsA"
                     /locus_tag="IV038_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP8340910.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(29794..30474)
                     /locus_tag="IV038_000668"
     CDS             complement(29794..30474)
                     /locus_tag="IV038_000668"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP8340911.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(30523..31158)
                     /locus_tag="IV038_000669"
     CDS             complement(30523..31158)
                     /locus_tag="IV038_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8340912.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(31224..32627)
                     /locus_tag="IV038_000670"
     CDS             complement(31224..32627)
                     /locus_tag="IV038_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP8340913.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(32614..33645)
                     /locus_tag="IV038_000671"
     CDS             complement(32614..33645)
                     /locus_tag="IV038_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340914.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            33676..33897
                     /locus_tag="IV038_000672"
     CDS             33676..33897
                     /locus_tag="IV038_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP8340915.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(34062..34799)
                     /locus_tag="IV038_000673"
     CDS             complement(34062..34799)
                     /locus_tag="IV038_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP8340916.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(34952..36292)
                     /locus_tag="IV038_000674"
     CDS             complement(34952..36292)
                     /locus_tag="IV038_000674"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP8340917.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(36529..38052)
                     /locus_tag="IV038_000675"
     CDS             complement(36529..38052)
                     /locus_tag="IV038_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP8340918.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(38430..39041)
                     /locus_tag="IV038_000676"
     CDS             complement(38430..39041)
                     /locus_tag="IV038_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8340919.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(39164..39289)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(39371..40087)
                     /locus_tag="IV038_000677"
     CDS             complement(39371..40087)
                     /locus_tag="IV038_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8340920.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(40093..40662)
                     /gene="scpB"
                     /locus_tag="IV038_000678"
     CDS             complement(40093..40662)
                     /gene="scpB"
                     /locus_tag="IV038_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP8340921.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(40646..41443)
                     /locus_tag="IV038_000679"
     CDS             complement(40646..41443)
                     /locus_tag="IV038_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP8340922.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(41413..41784)
                     /locus_tag="IV038_000680"
     CDS             complement(41413..41784)
                     /locus_tag="IV038_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8340923.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(41803..42690)
                     /gene="xerD"
                     /locus_tag="IV038_000681"
     CDS             complement(41803..42690)
                     /gene="xerD"
                     /locus_tag="IV038_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP8340924.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(42786..43259)
                     /locus_tag="IV038_000682"
     CDS             complement(42786..43259)
                     /locus_tag="IV038_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8340925.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(43364..44227)
                     /locus_tag="IV038_000683"
     CDS             complement(43364..44227)
                     /locus_tag="IV038_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8340926.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(44423..46522)
                     /locus_tag="IV038_000684"
     CDS             complement(44423..46522)
                     /locus_tag="IV038_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8340927.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        12759 a         9817 c         7957 g        16145 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt tgggattgca
     1141 gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg tgcaattgct
     1201 tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag accatagttg
     1261 gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc catacttcca
     1321 atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg tgcggcgttg
     1381 attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc agtcatctga
     1441 cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag caattctggc
     1501 gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc attttctaac
     1561 ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt ctgaaagccc
     1621 acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac agcataagga
     1681 cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa aagcatcgtt
     1741 ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata tttttctttc
     1801 tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa agctttgaat
     1861 tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata gcgggacttc
     1921 aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc tgcatcatag
     1981 aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg atgagaagtc
     2041 aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc ttcgtttatc
     2101 actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa agaatcgcgg
     2161 tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt gattactacc
     2221 gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa actgtttcct
     2281 cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc ctcagtcgat
     2341 ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat ctgccaagca
     2401 gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga aagaacctct
     2461 tttctattat ttttggatgt cttcttcgta atctccattt acacagacta cttgtttacc
     2521 gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc tgcccactgg
     2581 tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga atagtctgtt
     2641 tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag tcacaccgat
     2701 ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag cataaaactt
     2761 cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat cgaatcctgc
     2821 tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca gttctttttc
     2881 aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt cttcaatttt
     2941 atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag ggaagaattc
     3001 acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt tttgtgtcaa
     3061 ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg aaggtcgccc
     3121 taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg ctggtggttg
     3181 agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt cttcaagttc
     3241 tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat acacttgcat
     3301 gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat tttgttctaa
     3361 tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa aacgtgacca
     3421 gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt catctggtgt
     3481 acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt gagcattttt
     3541 gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa taaatgctgc
     3601 ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt atgtaatcaa
     3661 gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg ctaccatcat
     3721 ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc tacttgtagt
     3781 gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa cttcgaagtc
     3841 tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta atttcttttt
     3901 cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt taaaattacc
     3961 gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac ggtcaataat
     4021 aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga ccttacgcca
     4081 gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag cttgctgtgc
     4141 atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga ctgcgtcttt
     4201 tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa gtaggtacgc
     4261 taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt aaactttttg
     4321 agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc gtatagaaat
     4381 ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg gtaaatctct
     4441 tatatggcca acactggcca ttactttata gtttcgtcca agatatttct caattgtctt
     4501 ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg aatggactcc
     4561 tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag ctaatcatat
     4621 ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat tctttaaatt
     4681 ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc aaatgatgac
     4741 atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt cttccaagat
     4801 ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca aagacacatg
     4861 ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc tttttcgggc
     4921 cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc tcttgcaagt
     4981 cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg attgtccgtc
     5041 caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg cagccactga
     5101 cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt tttttggcta
     5161 aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt aaagatagat
     5221 caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga gggggatatt
     5281 tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag ttagcgtgaa
     5341 tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct atcttaatga
     5401 tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca agtgcccatt
     5461 ttccttgact actgattcct tttgtataag caagtttcaa taaattttct tctatttgat
     5521 acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc ggctgttttt
     5581 tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt aattccttgt
     5641 cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga gaaaccatat
     5701 ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt agcaatgata
     5761 cttgcagcag caatcgacac agatcgtaca tcccctttga ttattttttc ctgctgaata
     5821 ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga caatttttct
     5881 actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc gattgtctgc
     5941 tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg ataaagaagt
     6001 tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat ctcatttttc
     6061 ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc tacttcatca
     6121 atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca aaacatttct
     6181 tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga agccagtgct
     6241 tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc atctattctc
     6301 tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac ttttttgctc
     6361 catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa tatcatgcac
     6421 gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg taatatccat
     6481 cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac gatcgctgat
     6541 tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat catctaaatg
     6601 gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt cttcatcttc
     6661 aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac ctaaacgaag
     6721 ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt tcttgcctac
     6781 tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca ttgcccgaat
     6841 agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca atgcttcttt
     6901 agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag ctaatgcaag
     6961 atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat ctgccagatc
     7021 cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta gcattggatt
     7081 acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca catatttcat
     7141 tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt ggatggtcat
     7201 tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt ttttcttttt
     7261 caatataaat tcactttttt attataaaga aattccatct attagttaag tgaaagtgaa
     7321 ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta ttttggtttc
     7381 tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag acgaacaaaa
     7441 cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg ttgctgatgt
     7501 caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag aattttctac
     7561 tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc tctctcatga
     7621 tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa caagtggtgc
     7681 tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa
     7741 agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag aaatgttttc
     7801 attagaagtc caagatctag acggaaatac attagaaccc gtatggatga agatgtaagc
     7861 agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata cgataagtat
     7921 tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata aaagcagtct
     7981 tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca
     8041 cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa tattttgaac
     8101 actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt gttgtcgacc
     8161 tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg tcctgcttat
     8221 tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata tggaccgatc
     8281 tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt tggttcagga
     8341 acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa tggcttattt
     8401 ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt gattatgatc
     8461 ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc agatttcaaa
     8521 gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca aataatggtt
     8581 gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc tgtttgattt
     8641 tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag tctcaacaga
     8701 ctgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga taatagacaa
     8761 atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga ctatcttttg
     8821 aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg gattgtcccg
     8881 ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct gatttcaggt
     8941 ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga tcattttggt
     9001 agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg ccatctgcta
     9061 attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc acaaccatat
     9121 caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg atgaataaaa
     9181 atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat acaagccaga
     9241 agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct ctttttttca
     9301 ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg aaataaaaat
     9361 aattctttca attaggctat tggacaaaaa acttttttga tcataaagta caaaactatc
     9421 tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa gatatccatg
     9481 gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta gctagagctg
     9541 tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa agcatcatgt
     9601 gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc
     9661 tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat
     9721 gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg gctggttcga
     9781 tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc ttactttttt
     9841 cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc
     9901 gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa agttctacta
     9961 attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct gtatgtccag
    10021 tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca gtatatcctg
    10081 acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc
    10141 ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc atccgatgat
    10201 tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt ggtatttgag
    10261 ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat gtcatcattt
    10321 actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa ttgatccaac
    10381 ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa tttatagttt
    10441 ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat agaaagactt
    10501 ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata taaatagaaa
    10561 tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt ttccaaataa
    10621 aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct gatgatttga
    10681 ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct ttccgtaaga
    10741 gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc taaaaatacg
    10801 gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta atttgttttc
    10861 tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca tcctccttct
    10921 ctaaccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc atcaattgat
    10981 aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt ttgatgccat
    11041 ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca cctacttcct
    11101 gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc ggcatttttt
    11161 ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg tcagtgatct
    11221 caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt gcggttttat
    11281 tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt ttcagtggct
    11341 ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc atcaaatcat
    11401 taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga atttcttctt
    11461 ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca tagttttctg
    11521 tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg ggaacgaatc
    11581 ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata gctatgtagt
    11641 gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc attagtgggc
    11701 cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt aataatggga
    11761 acaaaacgag gatcaaatgt cgtttcgtta atttcatttt ttcacctctt gtattcgatt
    11821 ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat
    11881 tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag gctgagggct
    11941 tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt accagtatgc
    12001 gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa taattcttgc
    12061 aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat accgatttgt
    12121 cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt atcgaacgtt
    12181 ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt catcaccacg
    12241 cgtatgttca aaacactaga cagcaaacca gttgttcggc tgattcttcc accttttacc
    12301 aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac ttttttgact
    12361 tcttcaagga ttttctctac tgttttacct tcttttgcta attcagctgc tcgaattacc
    12421 tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt cgtttttgtt
    12481 agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt catatggatc
    12541 gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagatgaa ttctccaatt
    12601 ggtggttggc ttgttttggg cagatttttt gctttcgcca tcatttccat aaatctttcg
    12661 ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga tagcggcatg
    12721 acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga atctgttacg
    12781 atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc ttattcatgt
    12841 taaactacat gtaagacaat ttctattttt agtataacag acaaataatg atttttcatt
    12901 aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga
    12961 aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat tgagtatcgc
    13021 gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc atattgtctc
    13081 ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt tgtttcattc
    13141 attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt cgatttttct
    13201 tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat ggctaggttg
    13261 gactttattt gtattaatct ggttgtttgc gattcttggc attatatata aagcgttgac
    13321 gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt ttatggggtg
    13381 gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatgactg gtgttatttt
    13441 gatggtagca ggcgggcttt cttacacgat aggcgcattc ttttatagct tgaattctat
    13501 ccgctatatg catgttgttt ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt
    13561 ctctgtttta ttctttactt aataaaaaaa ataaagctat ggatgggaca gctctaaata
    13621 acagctagtc tatccatagc ttttttatat cgtcaattcg cctgtcccgc tttttacagg
    13681 attctcatta cgctgatacg tttcaaattg atgcggataa cgattttttt catccatttc
    13741 tccttgacta gtattgatca gtgaccaatc agaccagtca acaggtggaa aataagcatc
    13801 tccatcgaat acatgatgga tgactgtacg gtataatatc gtacaaaatg gcagaaattc
    13861 ttgataaaca gctgaccctc cagcgatgaa aaagataccg tctgtttctt tttccagagc
    13921 aagcacgtct tccacactat gcatgacatg cacgccttct gctttatagg aagaatctct
    13981 cgtcaaaaca atcgtctgac gattcggcaa cggacggcta cccatccctt cgaatgtttt
    14041 tcgtcccatc actagtgtat ttgcttctgt catttgtttg aagaatttca gatcatttgg
    14101 caaacgccaa ggaagacggt cttcttttcc aatcaatccg ttttcatcct gtgcccaaat
    14161 tgcagctaac attttctcat cctttctgat tctaaacagc gataggagct ttgatcgccg
    14221 gatgtgggtc atatccttct aattggatat cttccatctc aaaatcaaat actgactgct
    14281 tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc tcggctcaat tgttctttca
    14341 tttgttcgat atgattgata taaagatgag catctcctaa tgtgtgaaca aattcgccaa
    14401 ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa cgcataactt gcaatgttga
    14461 acggaacgcc tagaaacaca tccccactgc gttgatacaa ttggcagctt agccgtccct
    14521 cttgaacata aaattgaaac atcgtatgac aaggaggcaa agccatgcta ggaacatctt
    14581 ccggattcca tgcagaaacg atcaatcttc tagaatcagg agtctttttg attgcttcga
    14641 tcacttgttt gatttgatcg ataaagccac cgtcttttgt ttcccagtga cgccattgat
    14701 agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct
    14761 tctcgcaaaa aagcttgctc tgctttttat attcttcatt gaattcagga tctgtcaaac
    14821 atctatggcc aaaatcagtc atatctggtc cattatagtc tggacttttg atgtagcgtt
    14881 caaatgccca ttcatcccaa atatggtttt tattttgtaa tagatagcgg atattcgtat
    14941 ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg
    15001 ttaacaaagg aaaccctttt tgcagatcaa accgcatttg gtatccgaat aagcttaatg
    15061 tgcctgtgcc tgttctgtct tctttacgat gcccttcttc caacaatttt cttcctaagt
    15121 caagatacgc ttgttccatc ctttttcctc ctaatcttct acccattcac tcaagtattc
    15181 ccaccgtgcc attttttctt caagttgtgc ttctgtttca gatacgtcgt tttgcaattc
    15241 ttgtaatcga gtgaaattat ctccttgatg attcatctct tcttggagca atgagatttt
    15301 ttcttcaagt tctgcaatct catcttcaat cgtttcccat tctttttgtt ccatgtaaga
    15361 taatttcttc ttttcagcag gttccttgct ctgtattttt ggtttcgctt tttcagcagg
    15421 ctgctgggtc tctttttgct tcagtaaata actggacatg tttccgtaat aaagttcttg
    15481 ttttccgttt ccttggaata ctaataactt atctgctacc ttgtccaaga aataacggtc
    15541 atgggaaaca gtgatcacag cacctggaaa acttctaaaa taatcttcca aaatcgtcaa
    15601 tgttgctatg tccaaatcat tggtcggttc atccaataat aaaacattag gttgctgaat
    15661 cagcagtttc aacaaaaata accggcgttt ttctccgcct gaaagtttgc ctatcagtgt
    15721 cccatgcata aatcttggaa ataaaaatcg ttcaagtaat tctgctactc cgatttgact
    15781 gccatctgct gtttttgctt cttcagcagc ttcttgtaag taagcaatca tccgtttatc
    15841 tggtgccatt tcttcatttt cttgtgtata atacgcaaga tgaaccgttt caccgataga
    15901 cattgtaccg ctatccaggg gaattctgcc agctagaata ttaagcaatg tcgattttcc
    15961 cgcaccattt ttaccagtaa tacctagtct ttcccgagat tgaatcaata aatctaaatg
    16021 ttctagcagt gtttggtcat ttatacggta actgccatct ttgatttcca gtactttttt
    16081 ccctaaacgt tgtgtcgcta ggttcagctc taaatcatct tcttggttta cttggtacaa
    16141 gttctccttt agatcttcga aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg
    16201 tacgccagcc ctcatccaag ctaattcctg tttataaagc cgcttgcgct tttcttcttg
    16261 ttctacttct acacgatcac gttctgcttt ttctaagacg taagcttcat aatttccttt
    16321 atactcgtat aattttccaa atgaaagttc gaagattcgg tttgctactc tatctagaaa
    16381 ataacgatcg tgggtaacca tcaatagtgc gccacggtat tgtttcaaat agttttccaa
    16441 ccactcgatc gcttcataat ctaaatggtt tgttggttcg tctagtaata gtaaatcggg
    16501 ttcatcaatc aacacttgcg ccagactaac tcgttttttc tgaccacctg ataaagtact
    16561 gattttttta tctaattcac tgattcctaa tttttgcaaa ataatctttg cgttggtatc
    16621 tgtagtccaa gcatcttctt tattcatttg ttcttctgct ttagcataac gtttttgcac
    16681 atcagggtct tctccgtttt cccctagttc gatcaatgct tcctcatatg ctcgaacagt
    16741 ttggatcaag gggctgtttc cttgaaagac tgcttggaga acagttaatt catcggaaaa
    16801 ttcagctgct tgagaaagat aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg
    16861 atcaccgtcg ctgctatcga taccggcaat aatatttaag agacttgttt tacccgtccc
    16921 gttcgtccca atcaacccga tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa
    16981 caaatctttt tcaccataag tttttttgag gtcagttact tttaattctt tcaacatgtt
    17041 ccctccttat cttggatatt gtatcataaa aatagtgaac ttcaggaaaa tttttttggt
    17101 atttgttctt tttgtgttct caaaaaaaga acaggaccga ttcgatcctg ttctgatatg
    17161 ttttgttttt tataaaactt gaaaattttt acctttttat tttattggac tcgttttttt
    17221 tgactttgct cgaaacgcaa atcggaaaga aaagttatca gatggtttgt tcatcgtata
    17281 agattctaac aaattgatat tcctgcgata gctgttcgca gaaaacgtcg tgattttttc
    17341 agcaacttcg tattcatcaa tgatttttct ttcaagataa taacctctta gcattttttt
    17401 cgtaaacaga ctatcttgct ggatcatcca cgaagaaatc aaattccccg agttcatttt
    17461 tattccttca agtttgcgtt gctggataaa aaattgaacc atcgtttcat cataaatatc
    17521 agtcaagtta tctaaacttt caataatcaa ctgagtattt ttttgatatg tctctctgat
    17581 ttcttggata tcttcatcac taataaatgt ctgacgatca gcatatcttc tttgccaaaa
    17641 cattttcgga tggagaacga ttcgcattaa cctacggaca acaatgaacc aaaagccgat
    17701 aaaagacaaa atctggcttg tgatagaatg ttcgaatcga gaaagaaaac gggagtaaaa
    17761 acgataacca ttagaagaaa gcttgcccga acgataactt tcatccaatc catcccgttc
    17821 gatcgacaaa atcagtgctt gtatttcttg tacttcttgc ttttcatcat cggttaaatc
    17881 ttccaagtac agatcacgta tccgttcttg ataattttct atcactgctt cagtggctaa
    17941 aaattctttt gtattacgat caatttcctt tatttctttt cgcaaaactt caacgacctc
    18001 ttctaaaatc atcaaagcat tcaaatccgt gcttttcgct tcctctgttt ccgtcagcat
    18061 aggcaagaca atgatcccta tgaccaaagt cactaagatg acacccgccg tcagaaacaa
    18121 aagcaaagac cgctggtaaa acatcatatt attgattgaa aatggcaaaa taaaaatcgt
    18181 agccaaactg actgttccct ttacaccgcc aaatgttaag atcaagatct cgttcaactg
    18241 ttttttgaac tttttactgc catctttgaa tacataaaaa agactgatag aaatgaatct
    18301 gatcacaaaa agcatgacag agattaatac gataatcgct aataaaagac cgtttggata
    18361 taaaccgtct ccccatactg gcgagaacac ttgcgtcaat tcgattccta aaaatataaa
    18421 tactaatgca ttcagtgtaa aagcaatagt tgtccatgta ctgtgggaca agctcgacaa
    18481 ttctgcatca aacacagaga ttttcctaaa accagaagct tggagaatac ctgctgccac
    18541 tgcagcaatg attccagata catcaaatgc ctctgccaat acataggcta gaaaaggaag
    18601 caaaagttcg atcagcagat accctgtgac atcttgtgct gatgcttttt cgatcaaatg
    18661 aattattttt tgtttgaacc agacaagcaa aaagccgatc aaagctcccc caatactaga
    18721 aactactaga ctcatccccg cgtttactgc tgaaaaagat ccggtgatca gcgcaccaag
    18781 agcaaactgg aaggcagtaa caccagatgc atcattcaaa agaccttctc cttccaaaat
    18841 atgcatcgcc tttttaggga tattcactcg tccagaaaga gaactgactg ctaccgcatc
    18901 tgtcggacct aacgctgctc caaaagcaaa gcaggcagca agtgggacac ttggcaacag
    18961 gaatgataac gtcgcaccta ccccgacaag ggtcaaaatg actcctccaa atgccaagaa
    19021 caaaattgtc ccgaaatttt tgagaatcga agaaatatct gctttttctc cttctctaaa
    19081 taacaatggt gcaataatca taactaagaa caattcaggt tcaaaatcga ttgattgtcc
    19141 ccactctgtc agacctaata ccactccaag gaaaatttgg atcagtggag ctggaatcat
    19201 tggcaaaatg cggctagcta tgttcgataa ggtgatcgtt actgcaaata caataactaa
    19261 ctcagcaaat tccatcgtcc atccccttta ttttttattt tgtgtaagca tttttaaaaa
    19321 ttgttctttt ttctttaaaa ttttggtttc gactacttca acctgtgttt ggttcccctc
    19381 taattcacac tgtacacgtt ttgactccaa acgaagcaga tcatgtttca gacgttccaa
    19441 ctgacgatac atcaatatat ttcttttacg caacttacaa agtatttgac ggcggtcatc
    19501 catcgaaagg gcgccatagc gatcatgcaa cagttttttt tgttgttcaa aactctctct
    19561 cattttccag atctcctata cacaaacttc catcattata gcaaagttct ttttggatga
    19621 aaaatatttc attgtttcat cgtttattgc tagttagaaa tttccataaa tttttcaacg
    19681 cctcattcta ttccaaatag tatcagatat caagtaaaac ttaagaaaga taaaagtttc
    19741 tgacaaaagg atgtttactt tcccattcct gcctttcttg ttcttttatc acaacaaaaa
    19801 aatggggctg cgacaaaaat cttgtcacag tcccttattc tgaataaacg gtgtggaaga
    19861 gacagcctat cagtagtaat aagacacatc tctttcacag cctcattttt gcgtaaaaat
    19921 cgatttattc gaaaaattcc ccgtatacat cttctacacg ttgatctgga ttgatcacga
    19981 tatacaagtt acctgttttt tcgctgattt ttgttgattg atattgattg tctaatcctt
    20041 ctgttccttt atctagatat gggaagtaca caagatcagg tgtgccattt cggtaaagga
    20101 tttccattcg ctcgatatct tcgtatttca atgcgcgatt aaacatgccc atttccagtc
    20161 cgcctaggtt gatatcatgt gtcgcaactg cgtccccttc atggaagatt tcgattttaa
    20221 atccagcaca cggatggatt tcaacaaatt cactgccatt gattcgtcca aaacttgttg
    20281 tgatttgttt gatccaaaga tcgccgatat gacggcggtt gatcgtccag gtttctccat
    20341 ttctaaaata aaattttagc gctgtcattt ctttactcat taaaaagact cctttatgat
    20401 gtaatttatt cttgtactac ctcagtttag cacaaattta tgtaagtggt cacatcttat
    20461 gttttttctt ctaatgacgt atctgcccac tttaagtagc caacaaagct gagcagatac
    20521 agtctagtgt tttttaaatc aatttttctt tcgataaaaa atccaaaacc tgttttttat
    20581 cattttttat tctttgttct acgacggctt tttcagctaa ttgcaaagct tcactaaccc
    20641 acttaccagg tttttttcca gtttcttcta gcaagatttt accatctact gctaattcgc
    20701 ttaatgactt gataggtaaa cgatcatagg attctcctac ggcatccatc tgaggtgttt
    20761 cgtcaaaata gatcatcaat tcttctactt gcagtgcatg ttttttgcct aagaagtaca
    20821 attcgaatgg ttccaaagct ccagtttctc gttgtcgtag tccttggaca agtgcttgaa
    20881 cgtctttaat catttggtta gaacacttcc acgatttcaa aaaggaacga atctcatttt
    20941 cttttagatc cagttggctg atcaataatg cccatgcttg gctctcttct ttcaatggga
    21001 cggatggtaa ttcagcaaaa cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc
    21061 aaatgtagca ctctgtttca ataaatgcat ggattcccgc ggcccgttca tttcccaaga
    21121 gcatcttgat gaattccaca tttatacgtt ctacagatat tttacctaat agctcgtggt
    21181 tttctttgat ggcgtagaag gtttcttcct ctaatttgaa ccctagttga ctgacaaaac
    21241 gcaacccgcg catcattcgt aaggcatctt catggaaccg ctcatgagga ttgcctacag
    21301 ctcgcaagat ctgttcttct agatcttgca gaccatcgaa caaatcgatg atttctccat
    21361 cttctttcaa agcaaacgca ttgatcgtaa aatctcttct tttcaaatct tctgccaacg
    21421 aacgaacgaa ttcgacatga tctggtctac ggtagtcctg ataagttgat tccgtccgaa
    21481 atgtggtgat ctcatattgt tcatcatttt ctaataccaa cacagttcca tgttcgatcc
    21541 caatatctat cgtttttgga aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga
    21601 tatccacatc atggattggc tggttcagca agatatcacg aacacttcca cctacaaaat
    21661 aagcttcata tcctgcattc tctaattttt tcagaactgg aattgccttt tggtattcca
    21721 gaggtaattg ttttaatttc atttttccaa cctttcctgc gtgtttcttg tcctgagata
    21781 aagactcagt tgcatggtga ctgtttgaca agtccgtttc agagtcttca aggccaggac
    21841 cacttcatct tgtttactcg tccttacgct caaagcgatt atggtctctt tgattgaatt
    21901 tttccatgtt tttttcaaaa acttcagtta agtcgatatt caatgaatta gccatgatca
    21961 tcgtaacgaa taaaacatca ccaagttctt ctgctaccga gttaactggt tcagaagcct
    22021 ttttgctttt ttctccataa gtgtggttga cttctcttgc caattcacct acttcttcag
    22081 ttaatcgagc catctgagct aatggagaga aataccccac cttgaattgc tggatatatt
    22141 catccacttc ctgctgcata ctgtataaag aacgcttttt atccatcttt ttccctccaa
    22201 aactatctta ccaaaacttt aacgcaaatc ctagataaga gcaattgaga aaatcgaatt
    22261 tccatgctac atttaggaag gaatttctaa atggaaattc tgtgaaaata cataattgaa
    22321 agctggaatt catatggtca aacgaaaaac agtaaaagaa gttcttttta tcattatagg
    22381 aacaagtatt tatgcatttg gtcttgttta cttgaacatc gctaatcatt tagcagaagg
    22441 gggagtctca gggatcacct tgatcttaag agctttattt ggtattgatc cagcttatac
    22501 gactttatta atcaacatcc cattgatttt actaggagga aagatcctcg gtaaacgttc
    22561 cttggcctat actgttctag gtacactttc tttgtcattc tttttatgga tctggcaaag
    22621 ggttccacta gaaatcaatt tacagcatga tctactgatc gtttctctac ttgcgggttt
    22681 agttgcaggt gtaggaagcg ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga
    22741 tattatcgct cggattttag aacaaaatta cggcatcact atggggcgtt ccctattggc
    22801 atttgatatc atcgtcttac ttgcttcatt aacctatatc gacttgaaac gtatgatgta
    22861 tacgttgatt gtttcgtatg ttttcagtcg tgtgattgat tcgatcttag atggcggata
    22921 ttctgcaaaa ggtctacttg tcgtctccaa caaaagcgaa gagattgcac cactactgat
    22981 gaccggtctg caaagaggtg tgactttcct ttctggagaa ggcgcattct ctggtaattc
    23041 aaaaaaaatc atctatatgg tcgttagctc tagtgaatta actgaagcta agaggatcgt
    23101 ccacgagatt gacgaaaaag ctttcctttc aatcatcaat gtgcatgaag tggaagggca
    23161 aggctttact tatttgaagc cacagacaag attactgaaa aaactataag aaaaaagaca
    23221 aagataggct atgaggaaag attccacatc cagctgacct ttgtcttttt tagtactata
    23281 ctatttttaa ctaattgtta tgttcactta cccggttact tttcttttgt gtctgcaaaa
    23341 ggatctttcg gttaacttca tcagataatt tcaaaaaagc atccctatct ccatcttcta
    23401 gtgcttgatc gatttgagca taaagcattt ttagttttgc ctcttcttct ttttcttgaa
    23461 aatatcgttc aattaattca gtatcttctt cactgactga agaatcccaa gaagaaaatg
    23521 gattgtcttc taagatagat agatattctc ttgtctgcca agcattttcg aatatacatt
    23581 ccacatataa aggctttttc caattcaaac ggatttcatg aaaaatctgg tctgtgtccg
    23641 taaattcttt tccctgcaaa aacaatttca tgggttcccc ttcaacagat agatcactga
    23701 tctgcagtcc tcgatccgca aaattcgccc cttcaacaaa gtggacattg cttaaaatcg
    23761 cttcatgatt ggcaagataa ttcaagatcc aaaaaacttc tcttcggcta aaagatacat
    23821 gattgaccat ccacgttaaa aaattcttct tttcccgtac atcaattagc atttttctca
    23881 cctctctgtt aggtcatcta ataatgacag tgcctccata tctcctggtt caagtgttaa
    23941 ataataagtc aataattctt gcgctctttt cagctgccct tcttctctta agaaaaaggc
    24001 atactctttc aagaaatctg gttcatgtga tagttcatga taagcctgtt catagtgtac
    24061 agctgcaacg gtaaagtctt ctaattcatt ataagcatgg gctaggttcc attctgcata
    24121 aggattagca gtttcttcca tctgattgat cacttgtatc acatcttcat atctttcatc
    24181 atccaagtaa agattactta aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa
    24241 agctttcagt aaaagttctt ctgctcgctg tctatcatgc agacggaaag cattttccga
    24301 ggccaggtga taaagttcga cttggtaagg attttctttg attccttctt ctaacactgt
    24361 ttgtgcttct tcaatcattt cttcttcttg aagagcttgt cctagatata gatataacga
    24421 ttgataatgc ggattaactt ctcgaagttc ttccagatag ttgattgcct gctgattttc
    24481 tttcaattga agattcgtaa aagctagctg gaacagtcga tcatctgtcc tttcttcttt
    24541 cagtgcttct tctaaaggta cgatagctgc ttcgaattct ccttgcatac ttaaggattg
    24601 tcctaaacgt tcctgcatcg agattcctga aatttcattt atcccgcttg ccaacaatgt
    24661 ttcataaacc actgctgcct ctttgaaacg atcaactgaa aaatacaatt cagctagcgc
    24721 aaactggatc aatggttcat caggaagcaa cccagctgct tctttcaatt ttgcttcact
    24781 cacttcaggg attccgataa cttggtacaa atcagctatc gccaataagc tttgcggata
    24841 gtaatcactt tgcttatcca ctttttccag gtattcaaaa gctaagtcga tctcattatc
    24901 ttcgattgca atttctgcta acgggatgtt cagtccgtca ttttctgggt attggttgat
    24961 cagttgttca aaaatctgtt ttgcttcgat caaaaagcca atagccagca attcttcgcc
    25021 tagttcagct aatatatctg ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc
    25081 ttgtgacaag tcttcatctg ataaagcttg caacattttt tcgctatttg tattcatttc
    25141 aattccctct tatctcactg acgatttgtt gaaataccgt cattgttctc aataattctt
    25201 tttcgtttgg atgttggtat ccgtctatta tacctatttt ttctaaaaca agtatttttt
    25261 tgcttttttc tgccaaaaaa agaacttttt ccaaatactc catctttgaa atctgttcca
    25321 gccagtggat tggatagcca agcaagtcca gccacacaaa aaagtttttt agttggaaat
    25381 caaaaggata tgcttggaga ttccacacga aatgaaacat cattcctaaa aattttttca
    25441 tactagcaga aagtagatgg ccattttcgg tcagataaaa agcttgttca aacagtttac
    25501 cataatttga caggttttct gcctctaatt gataaaaata gatcatttct tcgatcatcc
    25561 cggcaaacgg ccgagtcgtt aattgctttt tatttggata gtttttatac agattttgca
    25621 aaaaacgatg atcacaaatc attccgcaac agataaaagt caaaaagtca atctgctttc
    25681 cgtccacctg ccgctcgctg attgtttgat cataaacgat gcgttctggc agattgatgg
    25741 tttgcagcac tacacgattt ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc
    25801 ttagagaacg gatcgataca gggatcatcc atagtgatga atcaagggtt gtatgttttt
    25861 gaaaaaaacc tgccagatcc atgatccctt catttccaaa tccgatgata aacatggatt
    25921 gattttcggg atagcgtttg acgaaatcaa gcaggttctt taattctgtt aaatgattgc
    25981 attgagtgtt ggcacaaata taccagtcat tgtttgcttg atttgcaaat aacgggttga
    26041 tcttatctgc aaacaaatca taatagcgct gattcgttaa aaataagatt ggctgatcaa
    26101 tggaaagttc tcttagctga ctgactagcg tttctccgta aaacactttc gtcaacgcat
    26161 cttctacttg ataatcaagt tccatacgca tccctcctct tcttattttt attttaccat
    26221 tagtaagcaa attctagaaa agataagagc aaagctcacc tcataacttg caaataaata
    26281 aaaattaaat aaaatacatg tgttttctat gtatgacata cggattcgga atctctgtct
    26341 cttatttatt ccttttctgc taggaaataa aaagaaaagc acttgtagcc ctagagccac
    26401 aagcgctttt tcaactgtct tatttaacag cgtcttttaa tgctttacct ggtttgaatg
    26461 caggtacttt gctagctgga atttcgattt cttcaccagt ttgtgggtta cgacctttgc
    26521 gagctgcgcg ttcgcgaact tcaaagttac caaaaccgat taattgaact ttttcgccgt
    26581 tagctaaagc atcttgaata gttgaaaata cagcatctac tgctgcagtt gcatcttttt
    26641 tagttaaatc agtagctgaa gcaacttttt cgattaattc tgctttgttt gccatggata
    26701 gattcacctc ctccacaaga gatatgatag aaatatcatt tagttaaaac tttctgagtg
    26761 gtccagaagt ttaaaaagaa atattgatta acttcaatat tctgttatca cgatatcata
    26821 tgaaccgctt aatagcaagg tttttaccaa ttttcacatg ctttttttag tcatagaaac
    26881 gtaacaaacg aaactgtctt tttcttttga aaaaaaaacg tcgatatggt aaaatgccta
    26941 ttttctccta cgagggataa ttcggatgga cgtcccttca aaagtaaatg cttttctgat
    27001 ttgattttct aagaaacgtt cataagaaaa atgcatcaat tcctcttcat tgacaaaaac
    27061 gacaaatgta ggtggtttga cagcgacttg tgtagcataa aaaatcttca ggcgttttcc
    27121 tttatctgtt ggtgttggat tgattgctac agcatccata atgatatcat tcaacacagc
    27181 cgaagagata cgaagattct ggttcatact tacttgttcg atcatttcag gcagacgttc
    27241 caaccgttgt ttagtcactg cagaaacaaa aacgattgga gcgtaatcaa gataccggaa
    27301 ttcatcacga atctcttgtt cgaaatcacg cattgtattg gtttctttct ttacaagatc
    27361 ccatttattc acgacgataa taattccacg accagcttca tgggcgtaac ccgctactcg
    27421 cttgtcttgt tcccgaatcc cttcttcagc attcagtacc atcaaaacga tatcagaacg
    27481 gtcaatggca cgcattgcac gcatcacgct gtatttttca gtattttcgt atacttttcc
    27541 gcgtttacgc ataccagctg tatctatcat taagaatttc tgaccacttt cagaaacgaa
    27601 gtgggtgtcg attgcgtctc gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc
    27661 tcctaaaatc gcattgatca aagaggattt accaacattt gggcgaccaa tcaaactaaa
    27721 cttaatcgtg tcgtcgtcct cttcttcagt ctcttcagag aaatacttca ctgcttcatc
    27781 taaaatatca ccaatcccta gtccatggct tcctgaaaca ggataaggat cgcctagccc
    27841 taaggaataa aattcataga tatcgtttct catttctgga ttatctactt tattgactgc
    27901 taaaatcact ggtttattac ttctgtataa aattttcgca accatctcat ctgcgtctgt
    27961 cacgccttcg cgtccgctga ctacacaaat gattacatct gcttcttcaa tcgcgatttc
    28021 tgcttgatgc ttgatctgat ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc
    28081 gatcacgctg aattctcgac ctaaccattc accttttgca taaatacgat cacgagttac
    28141 tccaggagta tcttcgacaa tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga
    28201 cttaccgacg ttcgggcggc caacgattgc aattgttgga tttgccatat tatttcctcc
    28261 tcacaagttt ccaatctcac ttagtttaac gaatgcattg ccaactttca agcatagatt
    28321 gtcaattaaa cgaagtattt ttattcctta ttgaaatact aatcgctttt caaaagaaaa
    28381 actgagacaa aagcagctgc tttcatctca gtcacagata caaaatcaat tatttttcag
    28441 aatcatcggt tgctttttct tctacttctt ctttcaacgc atcacctaaa atgtctccca
    28501 tagtaaatcc agtattttct tctggtaatt cataagactc aacatttttt ggttcttctt
    28561 ctgaagctgg tttttcttct aaagctttga tactcaatgc aatacgatgt tcttctggat
    28621 ggatctctaa tactttcact ttcacttcgt caccttcatg aagcacttca tgtggtgtcg
    28681 caatatgttt atgtgaaatt tgagagatat ggactagccc ttctactcca gggaacactt
    28741 caacaaaggc accgaagctt gtcaaacgtt taactgttcc gtctaatact gtgccaactg
    28801 gagcttttgt atcgatttct gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa
    28861 cacgttcttg ttcaggattg atagacaaca ctttgacatt tacatcatca ttgacagcca
    28921 atacatcact tggttttgct acatgactat gtgaaatctc tgagacatgg actagaccgt
    28981 caattccgcc taggtcgaca aatgcgccaa aatcagtaag acgtgcaaca cgtccactga
    29041 gaacatctcc ctcatggata gttgaaagaa tttctttttt cttttcttct ctttcgtttt
    29101 ccactaccgc tttatgcgat aagatcaaac gattttctga tggttcgatt tcgatgattt
    29161 taaagctcaa tgttttgcct ttatagtctg aaaagtctgc aacaaaatga tcttccacca
    29221 ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac taaaccgcct ttgacaacat
    29281 ttgtaacagg tgcttcgatg accttgccct cttggaaatc tctttcgata tcttcccaaa
    29341 cttttttagc atctaaacgg cgttttgaaa gtagatagct gccgttttct ttatctttgc
    29401 cgatcgtact gataacgact aaatctaata catcaccgac ttttactact tcgctgatat
    29461 cttctaccgg caaagttgat aattcttttg ccggaacgac tccttcaact ccagcccctt
    29521 cgattccgac aatcacttgt ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc
    29581 caacttttac ttcttgtacg cttttcattg cgtcttccat tgactggttg ttctcttgat
    29641 tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa cattctgccg taagtacggt
    29701 actgttttta gtttaattga ttattcaaaa aaaataaagc gatttcgttc gttttccttg
    29761 aaaaaacaat tgtttttcat tttttttctt aatttaaaaa agaagataac cttttttcaa
    29821 aacaacagct tttattgctg caacgacttc tggaatcgat ttgccagtcg tatcgatacg
    29881 aacagcatcc tctgcttgct tcagcggaga gacgtccctt gttgaatcat aatggtctct
    29941 ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga attccttttt cttggttctc
    30001 tttgaaacgt ctttctgcac gctcacttac gcttgctact aaaaagattt tcacttctgc
    30061 attcggtaat acagctgtcc cgatgtctct tccgtccatg acaacgccac cgcgttcccc
    30121 tatttttcgt tggacagcaa ctaattcttc tctgactttt ccatgtgcag agacttcaga
    30181 aacagctttt gtgacatctg gttgacggat ttctaaagtc acatcttttg atgctatgaa
    30241 tacatgctgt ccatcttctt gttgcgaaaa agagatagga tactttttga tcagcgaaac
    30301 tagcccttct tcatctgaaa aagatacatt gttcgtaata gctaaataag ttactgcgcg
    30361 atacatggca ccggtatctg tataaatata attcaattct tttgctaata tcttggcaac
    30421 ggtacttttc ccagatgaag caggcccgtc gattgcaata ctgatttgtg tcatgatata
    30481 actccttttt ctcagaaaaa aagccgcaaa gcgcggctat tattatttaa ttcttaattc
    30541 ttgaccaggg taaagcataa agttgtttgg atctaagcca ttcaattcaa acaattgatc
    30601 agtggtgatc cctgcacgct cagccacttg ttttggacct tcgcctgctt gaacagtcgt
    30661 tgttgaaccg gctgcagctg atgaagaagg tgttgtttct gctgcattgc tgcttgaatc
    30721 ccctgcttga taagttgcct cacttgagga agtcggtgtt gtctctgctg caatactgct
    30781 ttctgacgtt acacctgtat cttgagacac agtagattct tccgcgattg tactagattc
    30841 tactgttgaa gtctcagttg actcctcttt tgttgtactt gtagaacttt ctactactga
    30901 tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg cttgatccat ttctgatcca
    30961 gaaataagca cctgctggaa tagcgataca taagaccagt aaaatcaaaa atagcgaaag
    31021 aaataatgta tttcctttct tttgttgtct ctgagaactt cttgatgaaa cttcatcatc
    31081 tgtatcgtag attggttgtt cccatggttc ttgtgtttcg ttattttctg tagattgatg
    31141 tctatctttt ctgctcactg ttttacctcc taaactctat cgtgtgtatt ataccatagg
    31201 aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt ttttaaataa atttagtaaa
    31261 atcgtttccc aatgaagttg ttcagtctta gaaaaaagct tcctctttgg cagaagttct
    31321 gcttttgctc catcaatatc acaacaattc tgcggctttt cggagagttt ttcgccaaaa
    31381 taagctaaga tgaattttct gcggcagttt tcttcattaa tatagcgaag catctgctgc
    31441 aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt
    31501 tcagactgct ggatcagctc catccatttt ttttgcagct catcaaaggg agcttgctct
    31561 gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag
    31621 aaataatgga tacgttcatc cccactttga taaaaaagaa ttgctgcact ttcttcctga
    31681 tctctgcctg ctcgaccgat ttcttgtaca tagttttcta gactgtctgg caaatcataa
    31741 tggaccacat aacggatatc agatttatcg atccccatac caaatgcatt cgtagcaatg
    31801 agaaactgca gctgattttt tacaaactgc tgttgaagtt gtcttctttg agctgcatca
    31861 agaccaccgt gatagtatcc aactgtaaaa cgttccctga ataaatgata caatcgttcg
    31921 acttcttttt ttgttgcaca ataaatgatt gccgctcctt gtgctctttc catgaagtgt
    31981 tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg
    32041 actgattgtc tgatctcttt tggtggtcga tcaaatagta catgacgtat atctcttgct
    32101 actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg
    32161 atttttccta actgttgata ttctggacgg aaatcaactc cccattgaga cacgcaatga
    32221 gcctcatcta ctacatataa cgcaatattt tgttttttca gatgctctaa aagagaagga
    32281 ttagtcaaca tttcgggact caaaaacaaa aatttatatt gcgatagatg acggaggacg
    32341 tatcttcttt cagattgcga aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt
    32401 ttttgtaatt gcgtcacttg atcttccatt agagaaagca gcggtgacac gatgatgacc
    32461 agaccttcca ttaaataccc cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc
    32521 aaaatagcga gagtatcttc tccatcaagc aacgcctgaa taatttcttc ttggccggga
    32581 cgaaatgaat caaatccaaa atattttttt aattcattta ttaacattct gtatcgcttc
    32641 ctttctcaat agatagattt gatacaaacg aaattctcca taatctaatt gtccatctac
    32701 attccattcg ctaaagcgcc actctcgcac ggaatcctta tttggttcca aacgttcgat
    32761 tgtttctgca gagagaatcc actcgtatgg aaaatcatca aaaaacaagg cccactcaat
    32821 aagatgatct gttactgttc ctctttttaa atgacggatt gccatgatct ggtcaattgt
    32881 ctgaccattc ataaacatct gcttggtgat catcatgctt tgattgaaat tttgctgtaa
    32941 taatgaatcg atcaaagcgt gccaaagcga atctggccgt ttttcgattt gtgctaacaa
    33001 aaggtggata catctgcttt gttgcagata aaccgctgac tcatcattgt tttttgccag
    33061 ctgataagga agcagccctg ttcgctcatt tccagataat tggttagcta aaaaatctgc
    33121 tgcttcttct ggtatctccg aaaacagctg caccaaactc tcatacgcag tatcaatcaa
    33181 aatttcacgt ggcaattggc tttcactaag ccattttttt agttggaaag tataaaaagg
    33241 acttgtctct gctggaagat agtcttgaat acccgttgcc agattagaaa ttacttgaac
    33301 agcaaattta atgagccgcc aactagtttc ccctgttctg ccatagcggt caaaacgcaa
    33361 accagtatgt tctatttgat gttcggacaa catatcagcc cctgcaggcg ttagctttgc
    33421 ttcattttca tttatttcga tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa
    33481 cttatcctgc ttcaattctg gaaaactgtt atggataaac aaaagttcgt gggtaaatcc
    33541 ataaataagt actgaagtcg ttcgttttcc cacaaggagt tggtaaagag aactaatccg
    33601 cagcttgtgt cctgatgaaa atagcgataa aataaaaaca atcatattat tctccttaag
    33661 ttaggatggt tagaaatgtc actactatgt aaacttgtac ctgaacgctg tattgcatgc
    33721 ggtctttgtc aaatcgaagc gccagatatt ttcgattatg atgaagaagg aatcgtttta
    33781 tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc cggaagaaga aacatatgta
    33841 aaaaaagcgt atcaaaaatg tccagttcga gcgattttat tagaaaataa aatataaata
    33901 taaacgaaac tttaaaaacg tacactaagt tctctaaact tgaaaataag ccgggatctg
    33961 tttctcttgt tccgtgaaat cccgacttat tttgaaagat tcatttgcta tctatagtta
    34021 attgactagt tttttcacgg accctatagg acccatttat attatttcaa ctttttcagc
    34081 cagttttcca atgtattgac gatcacagtt tgctggtctg cgttagaaat ttctgcctct
    34141 ccatctcctt tttgctcacc atagctgcca aaacctccat gatttccgcc attgattgat
    34201 acatactctg ttctttccgg taaatactgc ttagcttctt gataagcttc ctgattcaat
    34261 actttatctt ttgtcgccgt gatcgataaa acaggaacag ctgattctgt caatgttcct
    34321 tttttgtcgg gataacttgc catgaagaat acccctttta aatgtttgtc tgaatgattc
    34381 ttattgacat aacgacttgc cattacgcca cctaatgaat ggccaccgat cacataatcg
    34441 gaatttggaa aatcttcttt gaccttatcc gctttgtttc cgcccatcac tgctagatca
    34501 agtggcattt tgacaatagc gactgaaaag ccgttttttg ctaaatcttg cgcccaaata
    34561 ctataacttt ctggctctac gagagcacca ggataaaaaa taagtaatgg agactttcca
    34621 tgattctttt tctctccttc aaaaaataag taatcttctt tgttttttcc ttcttttacc
    34681 aacacttgcg cttctgacga tggttcgtac gtattttgct gtatatagta ataaccggct
    34741 ccaccgagta atcctaaaat gatgactaag ccaattagta ttcgtctaaa tattttcact
    34801 ttctcccacc ttttctatct gagtgacccc tttttcttaa tcaagtatga ttatacctta
    34861 aaaaaataag agagaaatgc aaactttcca gcatcaaata aaaaaagacg gaatatccat
    34921 ttgaatatcc gccctttttg tatgtttatt tttatttagc acataaacga tacacagctt
    34981 ctgcataaat ttccattgct ttatacatac tacttaattc ccatcgttca ttcgcctggt
    35041 gcataaagtc aggagtatca gggaacatcg caccgaatgc cacacactgg ttcatcgtac
    35101 gagcaaaagt agcgccgcct gatacaaacg gttcagtcat gtctccagta atatcgcgat
    35161 atgtccccaa tagatttttc actaactcac tgtctagtgg cacgtataat gaatcaagga
    35221 agtcaaattc ttcataagtc aacccatatt tttctgctgt tttagttaat ttagctacta
    35281 aatcatcttt cttgaatgta acagggattc tcatatctac accgattttt gtttgttctg
    35341 gtgtgatttc caaacttgca aagttcatcg ttagctcgcc ggattgttca tcttctgttt
    35401 tgcccacgac agcttcacca gttgcatttt cttgaactaa ttttcctaag aaattgattg
    35461 ggccaaaatc aaatacttct gaaagagcaa ttgccagacg agaaatagca ttcactcctt
    35521 gtgctgcatc tttcgcgtgg atacttttac caagaacgac gatgccttca ccttgttctt
    35581 cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc agaaagcttt tctccagagt
    35641 aaggagctgc atcaggaaca acattcaacg ctcctccagc ttttacagaa aactcgttcg
    35701 ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc tgcataaatt aaaggaaatt
    35761 cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt ttcgttatat ttttctaaac
    35821 aacgccataa attttcttca tctgtaccaa agataaaacg aatacgtgtc ttgaattcca
    35881 cgccagcatc aatcaaagct ttgacggcat acattgccgc aatagaagga cctttgtcat
    35941 cttgggatcc gcgaccaact aaccagccgt cttttatagt tggatcgaat ggttttgttt
    36001 cccaattatt ttcgtcccct gctggaacaa cgtccatatg acacaagata ccgaataatt
    36061 catctccaga gccaatctct gaataaccgt aatagccttc tggatcttta aatgttctaa
    36121 atcctagctt ttcactgatt tccaatactt tatccaaagc ttcaatcact tttttaccga
    36181 agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga aggaattcgg atcaattcgc
    36241 tcaatgattc taatgcttgt tcatgatgtt gttctgtcac aaattttttc atgatattat
    36301 cctcctaata tgttaaaaag ggtcatgtca aattcctccg acacaacctc ttcttctcaa
    36361 ataatgacgt tttaagcctg ctccatcagc aagcagaatg ttcttaacaa atgatgaaag
    36421 aaaaaacact cactgaatgg agctattgac ttatccacgt gcttatcgat agccgtttat
    36481 cggtttcttc attcttatga tagaccttgt acctgtattt gtctaggttt agaacattgc
    36541 tgctacacct aggatcacac agatggctac aaataatatc gcaattaatt taccggtaaa
    36601 tttccaccac actgaaacat cgaaacgagc aattgctaca gcacccatta cgatagcaga
    36661 tgttggtgta attaagttta ctaaaccaga tgcagattga taagcagtga ttactaaatc
    36721 cttaccgacg ccggcaaatt cacccatagg tcccatgatt cccattgttg cagcagctaa
    36781 gccggaagtt gaaggaatca agaatgacat agggatatag aaaataaatg taataatgat
    36841 gaatacactt gaagataatg aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt
    36901 gattaatcca ttgttcataa ccacttggat accacgagca acagctacta cgattgctac
    36961 accgatcaaa tcagaagcac cagctaaaaa ttcagagaca aattcagact ctttcatgcc
    37021 ataagtgatt gctactacga tggacattac taagaataac atagtaattt ctgggaagta
    37081 ccagtcacct aatggaagca tgcttttacc tagaaccgta cctagtaccg gaaggtttgt
    37141 taaccagttt gtcaatgatt caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat
    37201 caaactgatg atcatgatac caaaagtaat aaagaacaat acattaacat gtttttgttt
    37261 tttcgtgatt acttcttgac gaccgattgc ttcaatatcg aaatgcttca tatcttcttt
    37321 acgttggttg tatactaatg attttgttgg atctttttcg atctttgatg cataacgata
    37381 cacatataca atagaaatag ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc
    37441 ttcacccatc gaaatcccaa cagcttgtga agctacgcct gttgcaaaag gatttaccgt
    37501 tgaagctaga cagcctactt gagaccctac taatacgatt gctacagcta ctactgtatc
    37561 aaagcctaca gccatcatga ctgggatcag taaagcatag aatggaatcg tttcttctgc
    37621 cattccgtaa gtagagccgc ctaatgcaaa taacagcatc aatactggaa taagcatttt
    37681 ttcttttcct ttgaatttct tcacaactga agcaatcccg gcatctagtg cccctgtttt
    37741 gttgataata cctaaaaatc caccaacaac caaaatgaag aaagagattg ggattgcagc
    37801 aggtgtttct ttagttagct cccccgctgg tacacctagc atcccattga ttggcgccat
    37861 gaaaatatcc cacagccctt gaggattttg ttgtactcgt tcatatgatc cagcgacgat
    37921 gttgcctgca tcgtctacac tatagtgccc agctggtata aaccaagtaa gtacagcgat
    37981 tactgcaata ataataaata agaccgtata cgctgaaggc atttgaaatt ttttcttttt
    38041 tgtttgttcc atcttcttct tcctttcttt tccgttagag acaacaattt tggctctaat
    38101 gttattttat caaaaagagt atcagaacaa aaataataac acgcattttg taaaactttt
    38161 gttcaaaaag ttcgtttttc cctattccct cctctttttt caggttttta aagctttctt
    38221 ctttttcatt gtagcgtttc ctttttttta taaacaaaca ttttcgtctt ttaaaaggag
    38281 aagaatttca catttgcaaa gaattgattg atttttatgc ataaatcaaa aaaatactca
    38341 aaacaaacaa ataaaacgtt tacattattg catgtcttgt atttttttcc aaaaaaaaag
    38401 accaagaaat ttcttgatct ttttgatatt tatttgactg ctgtatgacg ttgttcgaac
    38461 tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat gcagcactaa aaagacagca
    38521 ctcacgatga atcctttgat caagttgaaa ggaacgatcc caatcagtac atagtttgct
    38581 aaagacatgc ccagcatctg tgttgcgttc agccctaaaa acattaagta aagaggcgta
    38641 ataacgaaat aatttgcgat actcataaaa atagttagag cgatggttcc tgtaatgaca
    38701 cctaaaaatt tgtttcttcc tttatgctgt ttctttacac taaagaaata ataaatcggg
    38761 aatgtaaaga tcgttgttga taagaaacta gcagtgtctc ctaccaaatt atccggagaa
    38821 aaacctgtag tcagcagatg taatactgaa cgcaagaacg ctgttgcaac tccggccgct
    38881 ggaccgaata agaacatgct gatcaatacc gagatatcac taaaatcgat cttcaaaaaa
    38941 ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac
    39001 atcgctactg caaccatttt ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg
    39061 actcctcttc agcgaacttt ttgacgtttc tctccaaatg aaaaaactct attttccagg
    39121 gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc
    39181 agactatact gtcggtatcg gaatttcacc gattcagcta tttttttgca aaataggtcg
    39241 tggactataa ccaccggtcg ggaatttcac cctgcccctg aagacgaacc ttatataatt
    39301 tttgtttaca tattcattat acatatctat tcaaaaaacg caacatctaa cacttataaa
    39361 aagtaagtgt tcattttgag tggttcagca aactgctgac ttcttttttg ttcagttcac
    39421 ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc gtatttttca cgttttagtt
    39481 tttgcacagg aagaccaact gcttgaagca tatttttcac ttgatgatta cgtccttcgt
    39541 ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc cactgataaa atttggaatt
    39601 tagctggaga tgtttttcgt ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg
    39661 attttgtagc gatccctttt acctttgcaa cgtaaacttt atccacttca tgttttggat
    39721 gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag caagatcccc gaagtatcgt
    39781 aatccagtcg acctacagga tagatacgtt cttttaccat cggcaaaaga tccgtaacaa
    39841 cttttcttcc tttgtcatct gcaacagcag aaataactcc tttaggtttg tacaataaat
    39901 agtaaccgta ttcttcttga taaattggga cttcatccac ttccacagtg tcatgtttcc
    39961 caacttttat ccctaattcc cttacaactt gtccgtttac ttttacacgt cctgcaagga
    40021 tgaattcttc tgcttttcga cgagaagtga tgccagcgtg ggcaattact ttctgcaatc
    40081 tctccattac aattattctc ctttattcat tccatcaaag aatagatctg gtgacacgtc
    40141 ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt tcttctaatg atttcaaacc
    40201 aaaataatct aagaagtagc cagttgttcc atataatatc ggccgacctg gtccttctac
    40261 tctacctttt tcttcaatca gtttatgggc agctagacgc tgtaccgctc ctgaaacctg
    40321 aactcctcga atctgctcga tttcagctct tgtgattggc tgtttataag cgatgattgc
    40381 taaggtttcg acggcggctt gagataatgt gtttgatgtc ccttgcgcat atcgcttaag
    40441 taagggagcc agctcttttt tagtcgttaa tacataatgg ctttccgttt caaaaatatg
    40501 caaagctgag tgcgagtttt gagtgtaaga ttcttttaat tctttcacta aacttgtgac
    40561 ttctgcttcg ctgagattta acagtccgct taattcttca ttactgatcc cttcctcgcc
    40621 aacaataaaa agaagggctt caagttcact taatttcgtc atgttcggta tctcctactg
    40681 ctggataaag cataattgat tcatagtttt ctttttgttg cgcaatgatc tgcccttttt
    40741 tcatcaattc tagcaaagcc ataaaagtag tgacaacttc acttttagaa ggtgactcaa
    40801 aaaaagaatc gaaagtacag ccatgcgcat ttctttttat acgctcttcg ataaatacca
    40861 ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt
    40921 tcttacgttc taagattttc tgcatggcac ggatcaaatc gatttttttg acttgtcctc
    40981 gcttcaacgg cttttcagca tccgctaacc catcgatgtt cataggttct tttgtgtaat
    41041 aaagacttcg ttcctctgct ttttctgtca gtctttcagc tgcatattta tatttacggt
    41101 attctaaaag ctgagtgacc aattcttctc gaggatcttc ttcccataat tcttcatcgt
    41161 ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt gattgccatc aatgtagcag
    41221 ccatcactaa gtattctcct gcaacagcta gctcgagtgt tttcattgta tggatataat
    41281 tcatatattg ctccgtgatc gtagcgatcg gtatatcata tatatcgatt tccatttgct
    41341 gaatcagatg aagcagcaga tcgagaggac cctcgaatac atctaattta atattgatct
    41401 ccgtcaactc attcactctt tcttcttttt ctttgctagc cattttccaa tgtaaggctc
    41461 tgtgatttca gttgcacgga tggagacatc cgggtacttt tcctgtaatt catctaacat
    41521 ttgaaagccc atattctcac cgcgataaga cggattcaaa gaaatgtcat ggatgatcag
    41581 ctgatcgttc gttgcccagt aaataccaat aatcccttga atgttggttg aaccttctgg
    41641 cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac gtatcaatct ccttcaacaa
    41701 agaagtttta cctgtcagtt tcttatggaa actcaatagt cccatagcaa ttttacggtt
    41761 ttctttccgg taatgtgtta acatcttctc accttcctat gactaagccc ttggaaaatg
    41821 ctgcttataa acatccgtca ttcttttctt cgtgatatgt gtataaatct gagtcgtaga
    41881 gatatccgcg tgacctaaca attcctgtac tgtccgcaaa tcagctccat tttctagcag
    41941 atgtgtggca aagctatgcc gcagcgtatg tggtgttact tctttgtaaa tcccagcctc
    42001 tcgtactaac tgcttcagat ttttccagat tccttgccga gaaagacctt tcccatgatt
    42061 attgacaaat acatgcattt cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa
    42121 gtagcgttcc agccattgga tcgcataatc acctaaagga atgatccgct ctttatctcc
    42181 ttttccaact gtctgtacca gtccaagaga aagatgaaga tctcccaatt tcaatccaat
    42241 caattcactg acgcgcatcc cagtagcata catgacttcc aagattgcac gatctcttat
    42301 ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt tctacttctg tcagagtcaa
    42361 tgtacttggt aatttctgtg ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc
    42421 agtgatacgc tcttgccgta aaaactgatg gaatcttctg agacttgaga tcatccgagt
    42481 aacagttgct gtcgcttttt tttcttcatg caacttctcc agaaaacgga tgactaaaaa
    42541 gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt agaaaatgca agtactgttc
    42601 caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta
    42661 gtggagataa tcgttgattt gctctttcat aataatcccc ctcatgcctc tctatcatat
    42721 cagataccgc ttcattctga tagtcttttt ccataatctg agcaaagaaa aagccgtcta
    42781 gacactcaga cggactggtt tcttttttct ttttcatagc attcctgaca gattccatgg
    42841 aacgttagcc gatgatcttt tactaaaaaa tgataacgtt cttctactac ttgctccact
    42901 tcaagtaaaa gatcctcttc tacttcctcg atcgtgccgc agttcaagca tagtaaatga
    42961 tgatggaaat gtttggcgcc ttcttttctc aaatcataac gggctaatcc atcattaaaa
    43021 cttactttat cgacaatttt caattctgtc aagatttcaa gggttcgata aacagtagca
    43081 agtccaattt ccggactctt ttttttcact aaaaagaaga tttcttctgc agataaatgc
    43141 tctttctcat tttccaaaag cacaagcaga gtagcttctc tttgcggtgt cagcttaaac
    43201 cccgcttcat gtagctgctg ttttgttttt ttcatcgccg aaatagcatt catgaccatt
    43261 tactcaaccc caacaattta taataattac aaataaaaaa agatataaca ctatgttaat
    43321 taaaattatt ataagctagt taagaaaaac atgcaaggga ttctcaatta gattttttga
    43381 ctagttttgt cacaccgttt tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg
    43441 cgcgcttaaa tgctcctttg ctgataccaa aagctttcat gatttcttct ggatccgatt
    43501 tatctgtaaa gttgatttgc tgatctgcag aacgttctaa aatggttaaa atcatttgtg
    43561 catcatctga tatagcttca tatgctcttg gcttcaatga aagattcagt acaccgtctg
    43621 gtcggacacc aatcactcga ccttttatct gctcgcctag acgaggttca ttatatcgtt
    43681 cagaaggatg gataaaccca agatagaaat cttccgttaa aacgtacgta cctgttaatt
    43741 tcaagcggta tgctgttcct atcacatctt tgtttttcat ttcttctgtt ccatatttgc
    43801 ttaaagattt gaatattttt tcatctgcta aactgcccca aatacggtct tttgcatcaa
    43861 ctctaaggga aatcatcaag cgatcccctt ttttaggcca tagctctgtc attgtcggca
    43921 attcatcaag agatacgaca aagtctttat ctggaagacc gatatcgaca aacgcaccta
    43981 aatcttttcg tgtagcagta acgattccaa aaccgtaatg accgatacgg cttttaggaa
    44041 tttgtgtagt gatccgattt tcatgttttt gattttggta accgaatcct tcaactgctt
    44101 caccgatttt gtgttcacct tctgatttgc ttaagccaaa ggtttgtccg tttttttgga
    44161 caaagtagtt tttttcattt tcatcaataa taatacctgt aaatatagtt gctaataaat
    44221 cgttcatttt tttctccctc tatagtgaaa ggttttctta atacatttct ttgctatcat
    44281 aacataatgt tatctacttg aatagctgag ccttggtttg gaaaggtgaa aaaaggagta
    44341 tgacaacagt tgtgtcatac tccccttttt cgaataaacg gtgttaaacg gcaggctcac
    44401 agcctctttc atttgcctaa aattaattgc tcccatttcc tccgaaccat ccagttttac
    44461 agttgatggt tacgatgtaa aatggcaaag cactttctcc aatgcctttc atcaatgcag
    44521 attttgttga agtggcatat agattataat agccttcccc attttcgatt ctggcttttt
    44581 ctaatctgta acctccttca acgatatatc cacgttggat acatgtagca atcactttgc
    44641 tttggacacc tggtgcccac gtccatgctg gattagatgc atcggcaata tcacttgctc
    44701 tggcagattt gattggctga ttcgctgagt ttgaagagga aggggtcgag gatgccgctt
    44761 cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc ttgagctgca gctgctgctt
    44821 gagcggcttg ttttgcttgt tcttcagctg cttttttcgc ttcttctgct tttttctttt
    44881 cctcttcttc ttttttctct ttatcagtga gcatcttatc tacttgtttc aattgttcat
    44941 tcaactgagc agatagatct gtatttttca ctttttctac tgcttctttt gccgtcttgt
    45001 attggtcgcg tgttgctgaa tccactactt ttccgtcttt aaagactata gctgtttttt
    45061 ctttagctgt ttgcaattca gcatattgat ttttagcttc tgtttgtgct tgttgaatca
    45121 attttccaaa atctgtgtta tccgtttcga ttgcttggat ctctttatct gctttcaact
    45181 tgatgtcatc tgctagatga tcatcgacga tggcaggtgc tatgaaccac tcattcactt
    45241 tttggatatc tcggatctta gattggatat cttggtaagt tgtttctaac tctgaatatc
    45301 ctttttcatt cttaagactg gataaatcat tttttagttt tgataaatct tgatgaatca
    45361 tatctgcacg aataaattga tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa
    45421 gttcttccat ttgttgctgt ctttttgctt ctgctgctac ttcggcttct actttttgat
    45481 gatggtcata ataagcccag cttcctccac cgagtagaag tactgctgct aaaacgagat
    45541 aaggtacttt ttttgatttt ttcttttgcg ctttatctgg atctgttttt tctaagtttg
    45601 attcttgatg tgttttttca gtatctggca cgattgttat tgatttcgct tctgcttcaa
    45661 acagcttagg ctggttctct tctttttcta tttttgcttc aagatcagta ccctctttgc
    45721 ttacagattg attttcagca tcggattcac tatttatttc atcgggctct gttgactttt
    45781 tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg cagtttattc gttttttcct
    45841 tttctaattg tttttcagta aatacttcag ttttttctgt gatatccgtt tttacgtctt
    45901 ttatttttac tgtttcttta cctgtggcgt tttctctatc agagtctttt tcagtttctt
    45961 gttccttttc tggctctact ttgttttcct gtgcaatttt ttcactgatc attttgagca
    46021 gctctttcgc gtgctcttct tttgtatctt cacgatgtgc tttgatataa gcggatagta
    46081 tcgaattttc ttcctcatta tcgacgattt ctttcagttc agctactctt cttttttgtt
    46141 ttgattcaag cgtttcttct tgattctttc ctgattcttc cgaagtctca tgaacagttg
    46201 ctttcttctg ttcatttttc tctatattag ttacttgatc gtgagcaggt ggttcttcgt
    46261 cttctggttc tgctttttcg aataattcca ttacagattc tagaggtaaa tctttcaatt
    46321 ccgaccattc gattggatcg tttaattctg tatcaggaaa gactgcatct tcagttgatt
    46381 tatcctgcat agctgcatct gtatttgttt cttcaattga ttcttttatt tgctgatctt
    46441 ctgcagttat ttgatgaccg cagtctggac agatagtatt ctgctccatt tttttcgatc
    46501 cgcaatttgg acattttttc atcacttagc gatcggctcc tttactaggt ttacgctacc
    46561 atttaatcta tcatattcta tacgtcacgc caatagctta tttttaggat ttacttaaaa
    46621 ataatgaatg aatttccttc ctatagaaaa aaaagcttga gactgcgtag tctcaagc
//
LOCUS       DACDCJ010000012        45145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDCJ010000012 DACDCJ010000000
VERSION     DACDCJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.012"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..378
                     /locus_tag="IV038_000685"
     CDS             133..378
                     /locus_tag="IV038_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8340928.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            550..1614
                     /locus_tag="IV038_000686"
     CDS             550..1614
                     /locus_tag="IV038_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP8340929.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1860..2285
                     /locus_tag="IV038_000687"
     CDS             1860..2285
                     /locus_tag="IV038_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8340930.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            2278..2727
                     /gene="nusB"
                     /locus_tag="IV038_000688"
     CDS             2278..2727
                     /gene="nusB"
                     /locus_tag="IV038_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP8340931.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2824..3678
                     /locus_tag="IV038_000689"
     CDS             2824..3678
                     /locus_tag="IV038_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP8340932.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3679..5016
                     /locus_tag="IV038_000690"
     CDS             3679..5016
                     /locus_tag="IV038_000690"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP8340933.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            5021..5245
                     /locus_tag="IV038_000691"
     CDS             5021..5245
                     /locus_tag="IV038_000691"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP8340934.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            5247..6137
                     /locus_tag="IV038_000692"
     CDS             5247..6137
                     /locus_tag="IV038_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8340935.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            6146..6964
                     /locus_tag="IV038_000693"
     CDS             6146..6964
                     /locus_tag="IV038_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP8340936.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            7071..7520
                     /locus_tag="IV038_000694"
     CDS             7071..7520
                     /locus_tag="IV038_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8340937.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7538..9220
                     /gene="recN"
                     /locus_tag="IV038_000695"
     CDS             7538..9220
                     /gene="recN"
                     /locus_tag="IV038_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP8340938.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(9275..9391)
                     /locus_tag="IV038_000696"
     CDS             complement(9275..9391)
                     /locus_tag="IV038_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP8340939.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9464..10420)
                     /locus_tag="IV038_000697"
     CDS             complement(9464..10420)
                     /locus_tag="IV038_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8340940.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10591..10959
                     /locus_tag="IV038_000698"
     CDS             10591..10959
                     /locus_tag="IV038_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP8340941.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            11129..11560
                     /gene="mraZ"
                     /locus_tag="IV038_000699"
     CDS             11129..11560
                     /gene="mraZ"
                     /locus_tag="IV038_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP8340942.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11581..12540
                     /gene="rsmH"
                     /locus_tag="IV038_000700"
     CDS             11581..12540
                     /gene="rsmH"
                     /locus_tag="IV038_000700"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP8340943.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12545..12946
                     /gene="ftsL"
                     /locus_tag="IV038_000701"
     CDS             12545..12946
                     /gene="ftsL"
                     /locus_tag="IV038_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8340944.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12947..15139
                     /locus_tag="IV038_000702"
     CDS             12947..15139
                     /locus_tag="IV038_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP8340945.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSVYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            15165..16127
                     /locus_tag="IV038_000703"
     CDS             15165..16127
                     /locus_tag="IV038_000703"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP8340946.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            16141..17502
                     /locus_tag="IV038_000704"
     CDS             16141..17502
                     /locus_tag="IV038_000704"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP8340947.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17528..18616
                     /gene="murG"
                     /locus_tag="IV038_000705"
     CDS             17528..18616
                     /gene="murG"
                     /locus_tag="IV038_000705"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8340948.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18631..19851
                     /locus_tag="IV038_000706"
     CDS             18631..19851
                     /locus_tag="IV038_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP8340949.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            20007..21332
                     /gene="ftsA"
                     /locus_tag="IV038_000707"
     CDS             20007..21332
                     /gene="ftsA"
                     /locus_tag="IV038_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP8340950.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            21355..22596
                     /gene="ftsZ"
                     /locus_tag="IV038_000708"
     CDS             21355..22596
                     /gene="ftsZ"
                     /locus_tag="IV038_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP8340951.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22613..23290
                     /locus_tag="IV038_000709"
     CDS             22613..23290
                     /locus_tag="IV038_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP8340952.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            23304..23903
                     /locus_tag="IV038_000710"
     CDS             23304..23903
                     /locus_tag="IV038_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP8340953.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23918..24205
                     /locus_tag="IV038_000711"
     CDS             23918..24205
                     /locus_tag="IV038_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP8340954.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            24254..25036
                     /locus_tag="IV038_000712"
     CDS             24254..25036
                     /locus_tag="IV038_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP8340955.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            25057..25764
                     /locus_tag="IV038_000713"
     CDS             25057..25764
                     /locus_tag="IV038_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP8340956.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            26026..28824
                     /gene="ileS"
                     /locus_tag="IV038_000714"
     CDS             26026..28824
                     /gene="ileS"
                     /locus_tag="IV038_000714"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP8340957.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28873..30399)
                     /locus_tag="IV038_000715"
     CDS             complement(28873..30399)
                     /locus_tag="IV038_000715"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP8340958.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(30414..31061)
                     /locus_tag="IV038_000716"
     CDS             complement(30414..31061)
                     /locus_tag="IV038_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP8340959.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(31245..31574)
                     /locus_tag="IV038_000717"
     CDS             complement(31245..31574)
                     /locus_tag="IV038_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP8340960.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31751..32479
                     /gene="sfsA"
                     /locus_tag="IV038_000718"
     CDS             31751..32479
                     /gene="sfsA"
                     /locus_tag="IV038_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP8340961.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32495..33508
                     /locus_tag="IV038_000719"
     CDS             32495..33508
                     /locus_tag="IV038_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8340962.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33508..34785
                     /locus_tag="IV038_000720"
     CDS             33508..34785
                     /locus_tag="IV038_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8340963.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34848..37550
                     /locus_tag="IV038_000721"
     CDS             34848..37550
                     /locus_tag="IV038_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8340964.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37702..38019
                     /locus_tag="IV038_000722"
     CDS             37702..38019
                     /locus_tag="IV038_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8340965.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            38049..38369
                     /locus_tag="IV038_000723"
     CDS             38049..38369
                     /locus_tag="IV038_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8340966.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38477..39937
                     /locus_tag="IV038_000724"
     CDS             38477..39937
                     /locus_tag="IV038_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8340967.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            40005..40226
                     /locus_tag="IV038_000725"
     CDS             40005..40226
                     /locus_tag="IV038_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340968.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            40257..40439
                     /locus_tag="IV038_000726"
     CDS             40257..40439
                     /locus_tag="IV038_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP8340969.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            40439..40852
                     /locus_tag="IV038_000727"
     CDS             40439..40852
                     /locus_tag="IV038_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP8340970.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40975..42156
                     /locus_tag="IV038_000728"
     CDS             40975..42156
                     /locus_tag="IV038_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340971.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            42545..42618
                     /locus_tag="IV038_000729"
     tRNA            42545..42618
                     /locus_tag="IV038_000729"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42579..42581,aa:Arg,seq:cct)
     gene            complement(42687..43826)
                     /locus_tag="IV038_000730"
     CDS             complement(42687..43826)
                     /locus_tag="IV038_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8340972.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(44125..44760)
                     /locus_tag="IV038_000731"
     CDS             complement(44125..44760)
                     /locus_tag="IV038_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340973.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(44873..>45145)
                     /locus_tag="IV038_000732"
     CDS             complement(44873..>45145)
                     /locus_tag="IV038_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP8340974.1"
                     /translation="KTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKVSYVIF
                     AKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        14948 a         7658 c         9558 g        12981 t
ORIGIN      
        1 aagaaaactt ctaagaggag gttttcttta tttttttact ttgcagaaga aaatatgaaa
       61 tagcaaaacc aattatttgt cccctttaaa tcccatatgc taaaattgat tagcaaagaa
      121 ggaggttata ttatgagttg gttatggaca ttgattgttg gagccgtgat cggagcaatt
      181 gcaggtgcga tcaccagtaa aggaaattcg atgggctgtg taacgaatat cattgctgga
      241 ctagtcggtt caagtattgg acaagcgatt ttcggcgaat ggggaccaaa agctgcaggt
      301 atggcactta ttccatctat tttaggcgca gtgattttgg tagcagttgt ctcatttttc
      361 tttggaaaac gatcataagt ttttacaaaa tagtaaacta aaaggagttg cagcgagact
      421 gtgactcctt tttggcttta tttatttaaa ataaaaagaa gttttccgga aatttttatt
      481 taactgtcgt gtacgaagaa aagtttgcta taatggaaaa aagcgtgcaa agaaagaggg
      541 agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa aaatgcaaga agaaaatttg
      601 gattcatttt tggttaccag tccgtacaat cttcgttact taacgaactt tacaggtacg
      661 acaggattag ctgttattac tttggaaaaa gcatttttta ttactgattt tcgttatacc
      721 gagcaggcgg cagcgcaggc tcaagggttt gaaattatta aaaatgtcgg acctattttt
      781 gatgaggtag caaacttagt acgcaaagaa gaactgcact cattagcttt tgaggaaaca
      841 acagtctcat ttttagaata ttcggtttta gaagaaatca tcgatgcgga attagtacct
      901 gtttcacaga tgatcgaaga actcagagaa gtgaaagatg aagaagaaat cgctattatc
      961 gaaaaagcat gtcatattgc ggatatggca tatgatcaca tcctgaaaat gatccaaccg
     1021 ggcatgactg agattgaagt cgcaaatcaa ctagactttt atatgcgttc tcttggcgct
     1081 tcaggtgttt cttttgatac gatcgttgct agcggattgc gttcggctat gccgcatggt
     1141 gtagcaagca aaaaaatcat cgaacaaggt gacttgatca cattggactt tggttgctat
     1201 tacgaagggt acgtttccga tatgacacgt acattcgcta ttggtgatcc tggggaaaaa
     1261 ttaaaagaaa tttatcaaat agtcttagat gctcaattag cagttattga tgctgcaaaa
     1321 ccaggaatga ctggtgtcca attagatgca gttgctagag attatatttc aaaacatggt
     1381 tacggggaag catttggaca ttctacagga catgggatcg gtttagagat ccacgaagga
     1441 ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag gaaatgtgat cactgatgaa
     1501 ccagggatct acttaccagg aatcggtgga gtacgtattg aggatgactt gttgatcaca
     1561 gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat tgatcatctt atagtttcat
     1621 caatagaaga agtaagcgta aaaatcagtg ttcacagact ttgttataga aaatcactcg
     1681 atttaatgat acactataga agaactttta agctgtaaga gataataaag ttgggtgttt
     1741 tctccaattc atagcagttt gcagctgatc ggcggcagtt ttcttaccac gtgatcacta
     1801 tgccgcatca tagagaattc ggatgtatct cgtgcttagg caaagggagg aataaataaa
     1861 tggcagatga aaaaaatttg attttaaacg caaaccaaga tttaggagag atcgtgatcg
     1921 ctcctgaagt catcgaagta attatcggga ttgcagcttc taaagtagaa ggcgtatacg
     1981 gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt aggacgtgca gctcacggaa
     2041 aaggtgttta cttagcaaac gaggaagatg gcttgaaagt agatctttac tgttatctag
     2101 aatatggtgt ttcagttcct aaagtagcaa tggaaatgca ggaacgggtc agccagcaag
     2161 tccttttcat gacggatatc gatttatctg aagtcaatat ccatgtagta gctgttgttc
     2221 ctgaaaaatt agaacaacca acaatcgaag aactttttga agacgaagag gaagagaatg
     2281 aataaagaat tatcaagaca cgagattcgg gaaatggcat tgcaggcttt atttccatta
     2341 gacttcaatg cagacctaac aaaagaagat gcgattttca acgcaatcga attagaccac
     2401 cgcgacatga tcaacgaaga tgaatcagag tttgttcctg tttatcttga tacgctagtt
     2461 ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga ttgaaaaaca tttaaaaaac
     2521 aattggcaca tcaatcggat ctcaaaaatg gatttggtga tcttgcgtat cgcgattttt
     2581 gaaatgactt atgtgacaga tgtaccagcg gctgttgctt taaatgaagc aatcgagttg
     2641 gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca acggtgtttt atccaatgta
     2701 ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt tctatgaatc atcataaacc
     2761 tgttataaat cgggaaaatt ctatggtaga atgaaaacac aaaaaatatc tgggagtggt
     2821 tagatggcag aattgattaa cggaaaagaa ttagctgaaa aaatgcaggc agagattgct
     2881 gaaaaaatcc aaaaattgaa agataatggt atccatcccg gattagtcgt tttattggta
     2941 ggggaaaatc cagccagcca aatctatgta agaaataaag aacgtgtagc aaaagaaatc
     3001 ggaatccatt cattggtaga gcgttatcct gagacgattt ctgaagacga actacttgca
     3061 gaaattgaaa aatacaatca agatcctagt tttcatggta tattggtcca attaccattg
     3121 ccaaagcata tagatgaaga aaagatcctg ttagctatcc gccctgaaaa agacgtagac
     3181 ggttttcatc cgatgaattt aggcagactc cttgcgggta aaccggacaa gattccttgt
     3241 actccatacg gcatcatgaa aatgtttgaa gcttattcta tcgatccggc gggtaaacga
     3301 gcagtagtta tcggacgaag caatatcgtc ggtaaaccga tggctcagct actcttgatg
     3361 gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa atctggctga actggcacaa
     3421 gaagcagata ttttagtcgt agcaatagga cgtggacatt ttgtcacaaa agaattcgta
     3481 aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg atgctaacgg taagctgatc
     3541 ggtgatgtga aatttgatga agtggagcct attgcaggct ggatcactcc tgtgccaaag
     3601 ggtgtagggc caatgactat cacgatgctg atgtatcaaa ccgtaaaatc tgccgaagct
     3661 tttggagcag gtgaatagat ggcacaagaa tatttaactg tcactacatt aacgaagtat
     3721 ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg tatatttaac aggcgaaata
     3781 tcgaatttcc gtttacgtgc caatgcccat caatatttca gcttaaaaga tgaccatgca
     3841 aaaatatctg ctattatgtt caaaggagca ttccaaaaat tacgattcca gccaaaagaa
     3901 ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg aagcgggagg ttcttatcaa
     3961 atctatgtag agcacatgga gcctgatgga ataggtgctt tatatcaaga attggaagaa
     4021 cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc ctaaaaaaat gctgccacgt
     4081 ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag cggttatccg cgatatcatc
     4141 acgacggtga aaagaagata tccaattgtc caattggttt tgttcccaac agttgtacaa
     4201 ggaaaccaag cagcggacaa tgttgtcagg aatatccaga gagtagaaga gatgggcaat
     4261 ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg aggatttatg gccgtttaat
     4321 gaagagcggg ttgctcgggc gatttatcaa gctaatacgc cggtgatttc atctgtagga
     4381 catgaaacag ataccacgat cgctgattta gtagcagatg ttcgggctgc cactccaaca
     4441 gcagctgcag aattagctgt tcctgttttg tcagaagagt tgatgcgcat tcaagaacgg
     4501 caagctagat tgcagcaggc gtttgtacgc cagatccagc gcaaacaaga acggttcgag
     4561 cgtgctcgac gttcctatgt atttcggcag cctgaaagat tgtatgaagg tcagaccatc
     4621 aaattagacc agttgaaaca gcgattatat caagctacac agcaaattta tcatgcgaaa
     4681 gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag tggctccgct ttatcgagtt
     4741 aaagcagctc gccaagaaac tgcttatcta gaaaaacgct tagaagagaa aatccaacaa
     4801 tatatgaaac aacagcagca gcgttttgct caagctgttc aatctcttga tttgttgagt
     4861 ccattgaaaa tcatgggaag aggctacagc tatacgacgt taaatgatca agtagtaaaa
     4921 agtgtcaccg agattcatca aggggatttg ctgacgatcc attatcaaga tggaatcgtt
     4981 aaaggacaag tacaagagat agacaaagga gaataaaaat atggaaaatc caacttttga
     5041 agagtcactg caagaattag aaaaaattgt catgcagtta gaacaaggag acgttccatt
     5101 agaatcagct ctagattcgt ttaaacgagg aatggaatta agcaaacatt gccaagatac
     5161 attagctaaa gcagaagaga ccttgacgaa aatgatgaca gaatcaaatg aagaagttga
     5221 ttttgacgga aacgaggaaa gctgagatga gattaactga cttttcagct attcatctac
     5281 ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca tacaacggat agaggactct
     5341 atgatgcgat gagttattca gtgaaagcag gcgggaaaag aatacgtccc ttattacttt
     5401 taacggccgt tgcttccttt gatgagccta ttgatgttcc cgtctatcaa gtagcagctg
     5461 ctttagaaat ggtgcatacg tattccttga tccacgatga tttgcctgcg atggacaatg
     5521 atgatcttcg tagaggaaaa cctaccaatc ataaagtatt cggcgaagca ttagctatat
     5581 tggccggaga tggtttgctt acaggagcat tccagctaat cagtatggca cacttaggta
     5641 attctcctaa gcttctgctg cttcagcaat tagctgtttg tgcgggatca caaggtatgg
     5701 ttgctgggca agctgcagat atcgagggcg aatcgaaaaa gctttcttta gaagaattag
     5761 catttattca tgaaagaaag acagggcatt tgattcgtta tgccttgctg gctggtggca
     5821 tattagcgaa gcaacctgaa gaaatcttat tgctacttca acgattagcc gaacatttag
     5881 gtttagcttt tcagataaga gatgatctac tcgatgtgat tggtacaacg aaaacattag
     5941 ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac ttacccgcgt ttattaggcc
     6001 tagaaaagac acgtgaggcg ttggagattg agctgttatc cgccaacaag atcatagaca
     6061 aattagaaga ggacgttttt tcttttgatg gcgggctgat gcgccagatg atcaagcaat
     6121 ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc gtagatgtat tagcggtcaa
     6181 acaaggatta tttgaaacaa gagaacaagc caaacgttca gtgatggctg gtttgattta
     6241 caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa attccagtga gtagtgaatt
     6301 aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg ggattaaaat tagaaaaagc
     6361 actcaaagca tttgatctat ctgttgaagg aaaaacattg ttagatattg gtgcatccac
     6421 tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa atgagttatg ctttagacgt
     6481 tggctacaat cagctagctt ggaaaataag acaagatcct agagtcgtcg taatggaaag
     6541 agtgaatttt cgttatgcaa aacccgaaga ctttacctta ggaattccag aagttgctgt
     6601 tatcgatgtt tcttttattt cactaaaact gatgctgcct ccattacatg ctattttaaa
     6661 agaagaggga gaagtgattg ctttaatcaa accccaattc gaagcaggca gagaagctgt
     6721 agggaaaaac ggaattgttc gtgaccctca gacccatcaa aaagttttag aagacatttt
     6781 gtcatttgcg gcacaaggtg gatatgatgt gctggaatta agttattctc cgatcacggg
     6841 gggagaagga aatatcgagt tcttggctca cttgaggaaa gtacccgaaa gcggaacaat
     6901 caattcagct attaatatgg cagaagtggt ttcaaatgca cacgaacagt ttgaccataa
     6961 ataaattgta actgtgaaaa tcaagtgaga agtatgcgga ttcttcttta ttataccaag
     7021 gaaataagct atcattgatt aagaagaata tgctaaggag cgaagacgta atgagaaaaa
     7081 aagatcggca tcgtttgatt actcgattgt tgagtgagca agatatccga aagcaggaag
     7141 aatttgttga aatcctgaaa agcaaaggaa tatctgttac tcaagctacg atttccagag
     7201 atataaagga actgaaactg atcaaggtac cggcttcaga tggcggctac cgttatagca
     7261 taccagctga aacaaacgaa gatgtaaata ctaaattgga aaaactatta aaagacgctt
     7321 tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa aacattgccg ggaaatgctt
     7381 cggcagcagc aaatttgatt gataaacgtt acaagaaaga attattttcg attatcaatg
     7441 atgacgataa tgttttgatg attacacgaa ctgaagacca ggcagtttta ttgaagaaag
     7501 acttcctgca ttacctttaa gttgaggagc gaatgtgatg ctacaagaaa tcagtattac
     7561 taactttgcg attattccgg aattgcggct ttcttttcac gaaggaatga ccgcgctgac
     7621 cggagagact ggtgcgggta aatccattat tatcgatgct ttaggtctgt tagcaggagg
     7681 aagaggttca agcgattata tacgtcaagg agcagaaaaa tgtgtgcttg aagggttgtt
     7741 tgaactacca aaacaagaag gattctctga actgatggtt gaattaggga tcgaaacaga
     7801 tgaggacaat ctgattgttc gacgggatat gtccttaact ggaaaaaatg tttgtcgagt
     7861 gaatggtcat attatcacat tagctaatct gagaagaatc ggcagttatt tagtagatat
     7921 ccaaggacaa aacgaacatc aggaactgct ccagcctgaa tcacatcttg ctttattgga
     7981 tcgttttgga gatgtagctt tccaacagaa gaaaaagagt tatcagcaag aatatatctc
     8041 ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa aatgaaaaat cctatgttca
     8101 gagaatagat atgctccatt tccagcaaga agaaatagct gctgctgatt tgcaagtcgg
     8161 agaagaagag aaattaaaag aagaaagaga aaaacttagc aattatcaaa aaattgctga
     8221 tggattagct gcaggttacg gagcactgac gaataatgaa caaaacagtg tagatggtgt
     8281 tggcttggcc gtttcagaac tgcaagggat cgcccatcta gatgtggagt atgaagcaat
     8341 ttatgaaaat atccaaagtg cttactattt gctgcaagat gcaatcggcg atatgagtcg
     8401 tcagattgat ttattggagt tagatgagag tcgtttagaa gaagttacgc aacgtttaga
     8461 attaatccga cagttgaaac gtaaatacgg cgaatccatt gagtcaatat tggcttacta
     8521 tgacgaaatc accgaagagc tagcttcttc tgatttttca gaaggtcaat tggacaaaat
     8581 gaaagaggaa ctagagcaaa aagagcttct acttcagcaa caagccgcag atcttcatga
     8641 agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt ctgcatgaat tgaaaagtct
     8701 gtatatggaa aacactgagt ttgaggtacg tttcttaaaa gaagaaaata gacaattgaa
     8761 tcatgatgga tttgaccaaa tcgaatttta cattacgacc aatccaggag agccactgaa
     8821 gcctttggtg aaagtcgctt ctggtggcga actgtctcgg atgttgcttg ctttgaaaac
     8881 tattttttct tctgagcaag gtgtgacaag tattattttt gatgaagtgg atacaggtgt
     8941 gagcggaaga gttgcacagg ccatcgcaga taaaatttct caaatatcga aatactctca
     9001 agttttgtgc atcactcatc tgcctcaagt tgctgcagtc gccgactatc aatattatat
     9061 tgtgaaagca gtgattgatg gacgtacgca aacttctgtt tcagagttga aaacaaaaga
     9121 aagagaagaa gaaattgcac gtatgctggc tggaagtgag attacaaaac tcacactcga
     9181 acatgcaaaa gaactattga aactcgcaaa aaggacctga gccacctcgc agtggttcag
     9241 gtccttttca gcagctgata gctatatttt atttttacat gacactcatt acagcagtca
     9301 atacaacaga tccaatcgtt aaaatcagca ttgtccaaat gacaaatttt gttactttag
     9361 caaaagtact tgtttgtttt ttttcacgca ttgttttcaa cctcgtcatt aattagttga
     9421 ttcaagtgta caatcttttt tccatttcca caagagggct tttttataaa aacttccctt
     9481 tttttaaata ataggtagtt acgatacaaa taaataatgt taatagaact agccatataa
     9541 aggcatcatc cctttcagca ataggtaact taatgttcat tccatagata ctgccgacga
     9601 ttgtcggtat agttaaaata atcgttaaag aagttaaaat tttcataata ttattcagat
     9661 tattcgatac gattgctgaa aaagtctgat tcatttgttc tactagtttt aattgaatgc
     9721 gagaagtagt cattgcctgc tctgtttcca ccaatacatc acgtaaatga tttttcaaag
     9781 catgattttc ttggaaaagt ttattttgcg ctagttcttt caatgcatct aaattagcag
     9841 aagtcgcttc tctgaaataa acgaggcttt tttggatatc catgatttga taaagttgtt
     9901 tgttttcagt ggatacttga agtttggctt ccaaagtatc gcattgagat tgaagcgtag
     9961 ctaaataact gttgtaagag agtgtgacct gccaaatgaa ttgaaagaag atattgagat
    10021 taggcgaaat atcacttttt ggaaaggtct ggctaaagac tttctttaaa aacagtggtt
    10081 catgattcgt aattgtgata agcttctttt tgggcgtcaa aataatagag agcgggtatg
    10141 tatccacttg aaaataaccg ctaggacttg ttttagcaaa aggatattgt aacaataata
    10201 acgctggctt tgtcaacttt gattgattga attcttctgt acgggaattt tctgcatcgt
    10261 ccaatatgga agtgatgtaa tctgacggaa tttcaaattg atgtgtcagc tgatgaatct
    10321 cttcttgatt aggacgttct acacaatacc agatcgtttg gtcagtttcc tttccggtat
    10381 tacgcagttt ctcattatct aatgtaaaat atttgatcat gattttcctc ctgttgcctg
    10441 tttcttttat tataacggat agggaattgg aatacatgga atgtgtttgt aagatataga
    10501 atataactgc ataaatttaa ataaaatgaa acagttgtgt aaagtatata cagtatactt
    10561 gaagcaacga taaaaaagga gtttacgttc atgggcacat tcacaccact tatcttgttt
    10621 tggtatattt acccggtaat cgttttgttt ggttgccaat ttcttgtaaa attattttcg
    10681 ttaaatcgtc gttttaaaat aaaagcccct gatttggcga ttcctttttt gtggggcggt
    10741 atgcatgcat tgtctagaaa tacagggaca atttctatct taccgttttt ctttatttct
    10801 gttttgctaa tgggaatttg tattgctgtt ttccaagcat attattatga agaaattatt
    10861 tatccgcgtt atttcaagat gacttggcgt ttggtttttc tgttgacaat cgtcttgtac
    10921 gctgtcttga tcattgtgaa catactttct tacctatgaa aaaaatagaa aagctgtaaa
    10981 aagtctttta aaaggctttt tgcagcttga tttttaaaaa aatgtttatg aataaaaaaa
    11041 gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt agactaattt
    11101 tagttagtgg aggaatgggg ggctttcaat gttgatgggt gaattccaac ataatattga
    11161 tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt
    11221 tctgacacgc ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct
    11281 ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt
    11341 cttctattcg gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag
    11401 cacgttaaga aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg
    11461 aattgaaatt tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt
    11521 tgacgaaatt gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta
    11581 atgactgaaa catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac
    11641 atcaaaccgg atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat
    11701 ttattaagcc aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca
    11761 atcgatcacg ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc
    11821 aaagcgaact tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc
    11881 attttgtatg atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt
    11941 tatcatcaag atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat
    12001 cacgtaatta atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag
    12061 gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc
    12121 caaacaactg gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga
    12181 aaaggcgggc atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa
    12241 ctcggtgccg aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga
    12301 atcagcgtaa tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa
    12361 tatagtacgg tacaagatct accaccggga cttcctgtca tacctgacga attccaacca
    12421 gaactgaaag tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac
    12481 aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag
    12541 aatgatggca gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga
    12601 tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc
    12661 accggcaaaa cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc
    12721 gattgctatt gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc
    12781 tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag
    12841 tttagaacaa gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga
    12901 aaaaggattg tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa
    12961 taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc gcaaaaaagt
    13021 aggtatcatt ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc
    13081 gtatatcgtt attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt
    13141 gtaccaagga agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt
    13201 ggcgctcgca gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac
    13261 ctctggggat aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct
    13321 gcataagaat ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa
    13381 aaaagagctg tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa
    13441 gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca
    13501 tcaggctcgg atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc
    13561 agacaaagat gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat
    13621 cttgagcggt aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc
    13681 aggtacagtg gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga
    13741 cagcagacta caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc
    13801 agaagaattg acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca
    13861 acgacctact ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg
    13921 taatttcttg gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc
    13981 agctgctatc gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca
    14041 agtagaagat gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg
    14101 tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg
    14161 acgatggtat aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga
    14221 tgatgaagta aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta
    14281 tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa
    14341 taacggtaca atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga
    14401 agagcgaaca actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa
    14461 agtccgtgaa tatatgagag atgttgtcga aagcgaaaac tacggtagtg tttatggtgt
    14521 atatagtgta cctggataca atgtatcggc aaaaaccgga acagcccaaa tcgcttctga
    14581 tacaggggga tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc
    14641 ttctgaagat ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg
    14701 aatggcattg gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac
    14761 tgaagaagat tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa
    14821 tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat
    14881 cggtaatggt aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg
    14941 agaaaaactg atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc
    15001 aaaagcagat atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga
    15061 tggttattgt gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag
    15121 ttttacttta gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag
    15181 cattgattcc cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg
    15241 gttattttca aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca
    15301 atagcaaagc cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga
    15361 caggaatttg ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt
    15421 ttgtattagc gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga
    15481 aaagaaacat gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca
    15541 tcttttatct tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag
    15601 atctaccttt aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa
    15661 atgcagtaaa tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt
    15721 ttgcagctta cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct
    15781 taagtgtttt aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt
    15841 ttatgggaga tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc
    15901 taaaccaaga atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg
    15961 tgatgcttca agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa
    16021 ttcatcatca ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt
    16081 tgatcagcat tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa
    16141 atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc
    16201 ggagtaagtg cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga
    16261 aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata
    16321 acaggtagtc atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca
    16381 gggattccct acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt
    16441 acggaagttg agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg
    16501 aacggaaaaa caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa
    16561 ggaattgcac gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca
    16621 aaatcagaag ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat
    16681 ttccgccctc atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt
    16741 acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat
    16801 tatctgatcc ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca
    16861 cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac
    16921 tcaatatact ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag
    16981 cataatgtag aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat
    17041 gaagctatac gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta
    17101 ggagaaatcc aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact
    17161 aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac
    17221 agaggaaata catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta
    17281 tttggccaaa caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata
    17341 gtaacagccg attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt
    17401 gatgtagttt tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact
    17461 agaggaaaca gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg
    17521 ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct
    17581 tgcctttgtc aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc
    17641 aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga
    17701 gattcaagga tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct
    17761 taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg
    17821 tactggagga tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac
    17881 agttatccat gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt
    17941 ggatcggatc gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt
    18001 tcttgtaggt aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa
    18061 aacatttgat ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc
    18121 acttaaaatc aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca
    18181 agttttgtat gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga
    18241 aatacaaaat attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag
    18301 tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt
    18361 accggcagtg ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat
    18421 gagtcttgtt cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc
    18481 actaagtcag acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg
    18541 agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca
    18601 aataatcaaa aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg
    18661 tctcatccgt cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat
    18721 ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca
    18781 cccctttcag attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag
    18841 tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat
    18901 aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt
    18961 cgtcggttag ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat
    19021 gtttctcctt atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc
    19081 caagaaatca tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt
    19141 catcgatcta cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat
    19201 atacatttca aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct
    19261 ttgatcgcaa aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa
    19321 aaagtagcaa atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa
    19381 atcaaagaat taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct
    19441 gaaatcaagt acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac
    19501 ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa
    19561 gtagccaaag atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat
    19621 ccttacggta gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt
    19681 gaagaaaatg cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa
    19741 acacctggat cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt
    19801 caaatagaag ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac
    19861 tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt
    19921 gaatatatct attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga
    19981 atagtaagta ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata
    20041 ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta
    20101 ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata
    20161 aaacggtcca agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga
    20221 tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa
    20281 tgattgcagt taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt
    20341 cagctgccct tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag
    20401 attttactgt cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat
    20461 tagaaatgta cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat
    20521 gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg
    20581 aatcaattct ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg
    20641 gccaaacaac aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag
    20701 aaggtggcga atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg
    20761 cagaagcgtt gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag
    20821 aatttccagt agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt
    20881 cagaaattat ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc
    20941 aaatcgaagc attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc
    21001 ctggtgttgt ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa
    21061 atcacatggg gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag
    21121 caaacttgag tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag
    21181 ctcgtcaaat ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg
    21241 aagaaacaat ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa
    21301 tcaagggttt ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa
    21361 ttctcaattg ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt
    21421 ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt
    21481 acagccaaca cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta
    21541 ggccccaaat atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg
    21601 gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc
    21661 actgcaggaa tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg
    21721 aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg
    21781 aaacgtggcc gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta
    21841 ttaattatct caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa
    21901 gctttccgtg aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc
    21961 acagcgccag gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa
    22021 ggtacagcat tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg
    22081 aaaaaagcaa tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt
    22141 cttaacatca caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc
    22201 gtagcaaatg cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa
    22261 atgggcgatg aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa
    22321 cgcaagtcta gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta
    22381 ttagatatgg atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg
    22441 gatatcagac gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc
    22501 gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa
    22561 ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc
    22621 tgacaacttg cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga
    22681 tccgaaagaa gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga
    22741 attggttaga gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa
    22801 gaaacaagat ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg
    22861 cagaaaggta aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag
    22921 attagcagaa gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa
    22981 tgtttctggt gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga
    23041 atctttagct gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga
    23101 agcttctgat gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt
    23161 tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga
    23221 ctttcaaatt gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa
    23281 agacgaataa aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt
    23341 tcggattagc tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg
    23401 ctgtacagcc aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg
    23461 cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc
    23521 agccggctga aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga
    23581 aagaaagaac agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca
    23641 ctgtaatgga accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg
    23701 gagaagcaac cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg
    23761 attttctaac aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga
    23821 tttttctttg tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag
    23881 atgcgaattt gtttgatctg taagaggagg aactacgatt tatactattg cactattact
    23941 tcatttactt caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct
    24001 ctcttggttt ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga
    24061 accgtatatc agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat
    24121 tttaggggca atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg
    24181 gataactgtc catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata
    24241 ggaggggaaa attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt
    24301 tatcgattcg gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac
    24361 cgaatttctt gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga
    24421 tttgcgtttc atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt
    24481 ccccgattat tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta
    24541 tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg
    24601 gatcaaacgc tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat
    24661 tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt
    24721 acgcttggaa ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga
    24781 aagaggaaca gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc
    24841 cagacagcgg tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa
    24901 tacaagacct gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag
    24961 agtccagatc caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg
    25021 tgtattacgt aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc
    25081 caaaataaga cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc
    25141 ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa
    25201 gcagtcaaac attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac
    25261 caatcaatca tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa
    25321 tccgaagtga tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa
    25381 aaacgtgcac atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat
    25441 gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga
    25501 gtattccatc aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg
    25561 gaatgggatg aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc
    25621 aaagaagttt tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca
    25681 ggagtcatag aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg
    25741 cctggtgaaa atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg
    25801 atgagagtgg aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct
    25861 tttccgaaat tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca
    25921 ctagatagat gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac
    25981 atgctttttt gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa
    26041 accttgcaat taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc
    26101 gagtggcaaa aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa
    26161 ggaaaaccta gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta
    26221 ggacattcat tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc
    26281 tatcgtgcac catatgttcc aggatgggat acacatggat tgccgattga acaagtattg
    26341 gctaataaag gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt
    26401 gaatatgcac tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca
    26461 ggagattggg aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc
    26521 gtatttggta aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg
    26581 tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct
    26641 ccatctattt ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca
    26701 gcttttgtca tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta
    26761 aatccaaaat ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa
    26821 gatttgctag aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa
    26881 tttgctggac aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca
    26941 cttgtgattt taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct
    27001 ccaggacatg gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca
    27061 cctgtagact caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat
    27121 gacaaagcaa atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta
    27181 gatttcttta ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg
    27241 gcaactccac aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta
    27301 gaaaaagtag agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac
    27361 cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat
    27421 gctgaaaatg gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt
    27481 gctgaacatg gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga
    27541 tttacccacc caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc
    27601 tggtttgatt ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc
    27661 ccagcagata tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc
    27721 acgactagtg tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt
    27781 gctttagatg gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa
    27841 aaagtgatca aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat
    27901 gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa
    27961 atcagaaata cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat
    28021 actgtttcgt acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa
    28081 gtaatcaaag aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt
    28141 acagttatga acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat
    28201 gtagtttata ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat
    28261 caaacagctg tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa
    28321 atctggtcat tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat
    28381 gaagagtttg caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt
    28441 gataatgtct taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg
    28501 gaagcaaaac taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat
    28561 gcagatattg cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat
    28621 gaacaagtgc cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca
    28681 gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa
    28741 cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta
    28801 gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac
    28861 ccccttttgt tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca
    28921 gatccattcg aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc
    28981 agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac
    29041 gatatgccaa gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa
    29101 cgcatctaat agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt
    29161 tctaaattcc aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa
    29221 ggataaagaa aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt
    29281 cgtatcgtca catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa
    29341 tacgatgttg atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg
    29401 aacacctgac caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc
    29461 tgattgagca tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc
    29521 accttgagca tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg
    29581 gatagctttc aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg
    29641 cggttccatt gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag
    29701 tgcgccacta tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt
    29761 aatttggata ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa
    29821 acggatagct gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat
    29881 atcttgttct gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc
    29941 agaaccgaac ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg
    30001 tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac
    30061 acggttaccg tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg
    30121 ataatgcgtt gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt
    30181 tgcttcttca tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg
    30241 ctcatcgctc cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc
    30301 ttttctgtaa aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc
    30361 gccgaatatc gtaaacaata catttttttc attagacatg tggatacact cctttatatt
    30421 ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt
    30481 tggatagtga tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata
    30541 ttcaggtcaa tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata
    30601 cgcacttttg taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact
    30661 tcctcttttt taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt
    30721 tctaaatgat ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg
    30781 acttcattcc agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg
    30841 cgaatcagac tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg
    30901 gaatgttcta caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct
    30961 gagacatcta gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt
    31021 tctaaaatga ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg
    31081 gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat
    31141 aaaaaacaaa ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact
    31201 cattctttcg tttataaagg atatatatat acattgggag ggatctattt ttcagataac
    31261 aacttatatg aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata
    31321 agataaaaac catactgatc acgtacgact aaacgatatt catctatttc taccacagat
    31381 gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt
    31441 ttttgctggc tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt
    31501 tccgcttgtt ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca
    31561 agaaaataga tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag
    31621 acatattctt tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt
    31681 tcgactggtt tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag
    31741 gtgacataat ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg
    31801 gtttatagca cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac
    31861 aggtagagga aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg
    31921 acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa
    31981 tatcgatagt caattgccta atcggctggc aattgatggt attttagacg gcacgatcct
    32041 tttaccagga ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc
    32101 caaatttgat atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg
    32161 tatgacactt gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt
    32221 gaaacatata ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt
    32281 tattgctcag ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact
    32341 agctgatatg gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca
    32401 agtatcggca gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt
    32461 tgaagatcct atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac
    32521 aaattggatt actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac
    32581 agcggtatat tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga
    32641 tatcgaattc gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta
    32701 tattgcttct ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa
    32761 aaatgtgata gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca
    32821 tttggcaaat gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg
    32881 ttttaaaaag attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag
    32941 ctatatgaaa tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt
    33001 ctcgactcat ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc
    33061 tgttgcttgg tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg
    33121 tgtagatggt cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc
    33181 aggtaaaatc ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt
    33241 gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat
    33301 cattgatttg gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc
    33361 caatgtcatg gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga
    33421 gctagctaga aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt
    33481 cgatgcagat aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat
    33541 tggcagaaaa aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt
    33601 tttcagccac taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa
    33661 acactggctt atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg
    33721 gtcatttttg cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg
    33781 ggaaaagaca aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag
    33841 attgagggtt tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa
    33901 ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca
    33961 gaccagctat ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca
    34021 acagagtatc agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa
    34081 aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa
    34141 ggactgagac attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt
    34201 ctcacgcata tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc
    34261 gtaatggaag aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag
    34321 aacaagctac tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt
    34381 ttgaccacag atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat
    34441 gcagaagttc cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg
    34501 ggtaatgaag gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg
    34561 cttttacgtc catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa
    34621 actgagcagc caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact
    34681 ttttctggaa aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa
    34741 aataaatcaa aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct
    34801 atgattatga aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc
    34861 aagaagagat cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag
    34921 ctgagcgctt aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg
    34981 cccatcatat tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt
    35041 caaaatctga tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg
    35101 gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac
    35161 cgcgggggtt gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg
    35221 aatgtatgta tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt
    35281 ctttcaattg tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga
    35341 tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag
    35401 acggcggtat attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc
    35461 tgtttacttt cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg
    35521 aagcatctgt tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt
    35581 tcaatcgacg gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg
    35641 agcgttatga aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta
    35701 tcgatatcga aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg
    35761 gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc
    35821 atcatgagaa aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat
    35881 tactgaaaat caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt
    35941 atttgacatt tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca
    36001 tgcaggtcta taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga
    36061 acattgatct agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca
    36121 aagaattgac taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa
    36181 ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc
    36241 gttttgcttt tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga
    36301 tcgtccaccc agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa
    36361 tcgatctttc aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg
    36421 gctttattag tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca
    36481 aagtagatgt tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg
    36541 cacaagaact attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag
    36601 ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga
    36661 atggcctact cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg
    36721 aagagactgg cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag
    36781 cgatacggat ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt
    36841 cctttgaatc gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta
    36901 aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc
    36961 gtattatgcc agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac
    37021 gtgaatcatt caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg
    37081 cgggaacagt tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg
    37141 atgatgaaaa actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa
    37201 ttgtcgattc acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt
    37261 cattgcaaaa gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag
    37321 tagatactgg aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa
    37381 gccatcggca atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata
    37441 tcgttcgggc aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg
    37501 atatcgcatt tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat
    37561 catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga
    37621 aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa
    37681 aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg
    37741 atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa
    37801 gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc
    37861 gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt
    37921 gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga
    37981 gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt
    38041 ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg
    38101 gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt
    38161 tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa
    38221 cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc
    38281 acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg
    38341 aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa
    38401 aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta
    38461 taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt
    38521 tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac
    38581 actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt
    38641 accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc
    38701 cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc
    38761 cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga
    38821 tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc
    38881 attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc
    38941 tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa
    39001 actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt
    39061 cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc
    39121 accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat
    39181 tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt
    39241 gcaaaaatat attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat
    39301 cgtcacagta tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc
    39361 accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa
    39421 aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc
    39481 ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac
    39541 gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc
    39601 taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc
    39661 aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc
    39721 aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg
    39781 ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat
    39841 cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc
    39901 aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc
    39961 tttaagtttc gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca
    40021 gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga
    40081 cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc
    40141 cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc
    40201 tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga
    40261 taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc
    40321 cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag
    40381 gaatctattt cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat
    40441 ggaaatcacg aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc
    40501 tgtccttttc gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa
    40561 tttggagtac gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt
    40621 cattgaaaac cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca
    40681 ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga
    40741 gtcttatggc atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt
    40801 taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga
    40861 aacatggtac tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa
    40921 gatagatctt tcctgcgaat cctcactaac ttttttgtag gaaggggcag caaaatgaca
    40981 aacaaagtaa gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat
    41041 gcatttaatg gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat
    41101 tcttggaatt acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc
    41161 ccttttccag tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct
    41221 tcttatccag aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa
    41281 gattgccgtg atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat
    41341 cggcgctatg gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat
    41401 gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa
    41461 gctgcaatca gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc
    41521 gaagactggt ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat
    41581 tttgatgata atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt
    41641 attattgcgg agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta
    41701 agttctcaca atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat
    41761 caaaattatc tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg
    41821 aggatcgtag atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca
    41881 tttgccctac aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta
    41941 acaatcgaaa atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt
    42001 cgaggaagta tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg
    42061 gtattccaag aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg
    42121 ccacagcaac agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt
    42181 tttgtcacca cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct
    42241 cttttttcgt aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt
    42301 cttggattgc ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg
    42361 cgtatacttt ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt
    42421 ctacctctag tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct
    42481 gattttttta gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga
    42541 aattgttcct gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt
    42601 caattccagt caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt
    42661 tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca
    42721 gtattgattt tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac
    42781 attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct
    42841 tgataaagta aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca
    42901 atccctaatt cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat
    42961 ccatttttta agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat
    43021 aattcttttt gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa
    43081 attttcctat ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat
    43141 gttttattaa tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga
    43201 agttcagcaa atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac
    43261 tgcgcagaaa ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa
    43321 tatttatctt cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga
    43381 tcaaacggaa ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta
    43441 aacttaataa cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat
    43501 cccactctat ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac
    43561 gtatcttcgt attttctata agtgatagga gatactgttc cttctttata aagttgcatc
    43621 caatcctcaa agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc
    43681 aagttatatt ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat
    43741 tttcttatct tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta
    43801 tttccacgtt tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg
    43861 aactataaag aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc
    43921 ggcgcaagga tgtgctattt gtcgttttaa tggtataatt ggatgtgtat tagatcatcc
    43981 aatcagaggt gtttattatg attaaagtga tttttaaaag aataactgaa cttccagagt
    44041 ttagattatt tgttgattat tggtatgtct ggttttttat tgttgcattg ctgataggtc
    44101 tagatgtcat attagaaaga aaacttaagt aagagttgag ttaataaatt gaattcctaa
    44161 atgagatatt ttgtagccca tattttgttt gccgacatca cctaatacta tggagctaat
    44221 cgttttttct aacagaccaa aatcagataa tctatttaag ctaccatcta ttagctcttg
    44281 tgaatagcca gacatgctga tatttccagt gaaattttcg tttccacgac tcattaagca
    44341 tgctaacaat tgaatctcaa tcgatgttaa ttgattcagt acaagaagat aatactcctc
    44401 gttatcccat gaagcgttgt taattcgaag taaacaattc ttataaagat ttttatacat
    44461 atctatttta ttttgtgcct ttgatttttc tacctcgtca tttatccctt cgaatattcc
    44521 aatgagttgg tccttgttct ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt
    44581 ttctaatgat tcgtttaaat ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt
    44641 tttagaccca aaataaagtg actgcacaac gccaccaata taaggaattg ctccaatacc
    44701 gccttcaact attaattcaa gatattcttt tttggttagt ttttcattgc tattatccat
    44761 agttacctcc tattttgtca tttcctttac aagcattgtg actcctgatg gcactctttt
    44821 gtataggttt tattattgtg cacaatctaa ctcttagtgg ggcgtggttc atttattttt
    44881 tacttacttt actattaatc atatcccatt gagcatagga tttttctatg ttcgatcctc
    44941 ttgaactaat ttctcctgta aaagttactt tatcgccctt tgtaaaggta tcagcattgt
    45001 ttaagtcctt cgcaaaaatt acatatgaaa ctttaggtgt tgcagaaata tcattccaac
    45061 ttttatcatc gtactctttg tctgctataa tggctattct agtagtcatt gattcgataa
    45121 cagttccttc aaaagtatat gtttt
//
LOCUS       DACDCJ010000013        43568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDCJ010000013 DACDCJ010000000
VERSION     DACDCJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.013"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(212..2593)
                     /locus_tag="IV038_000733"
     CDS             complement(212..2593)
                     /locus_tag="IV038_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8340975.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2583..3230)
                     /gene="recU"
                     /locus_tag="IV038_000734"
     CDS             complement(2583..3230)
                     /gene="recU"
                     /locus_tag="IV038_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP8340976.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3294..3833
                     /locus_tag="IV038_000735"
     CDS             3294..3833
                     /locus_tag="IV038_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP8340977.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3923..4342
                     /gene="gpsB"
                     /locus_tag="IV038_000736"
     CDS             3923..4342
                     /gene="gpsB"
                     /locus_tag="IV038_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP8340978.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4364..4731
                     /gene="rnpB"
                     /locus_tag="IV038_000737"
     ncRNA           4364..4731
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IV038_000737"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4940..6106
                     /locus_tag="IV038_000738"
     CDS             4940..6106
                     /locus_tag="IV038_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP8340979.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6152..7648
                     /locus_tag="IV038_000739"
     CDS             6152..7648
                     /locus_tag="IV038_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP8340980.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7729..8412
                     /locus_tag="IV038_000740"
     CDS             7729..8412
                     /locus_tag="IV038_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8340981.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9057..10247
                     /locus_tag="IV038_000741"
     CDS             9057..10247
                     /locus_tag="IV038_000741"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP8340982.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10349..11302
                     /locus_tag="IV038_000742"
     CDS             10349..11302
                     /locus_tag="IV038_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP8340983.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11371..12720
                     /locus_tag="IV038_000743"
     CDS             11371..12720
                     /locus_tag="IV038_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8340984.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12903..13226)
                     /locus_tag="IV038_000744"
     CDS             complement(12903..13226)
                     /locus_tag="IV038_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340985.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13355..14521
                     /locus_tag="IV038_000745"
     CDS             13355..14521
                     /locus_tag="IV038_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340986.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKNGFCFMKKLVTHILLARLAKQRMMILEIS"
     gene            14587..15936
                     /locus_tag="IV038_000746"
     CDS             14587..15936
                     /locus_tag="IV038_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8340987.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15981..16412)
                     /locus_tag="IV038_000747"
     CDS             complement(15981..16412)
                     /locus_tag="IV038_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8340988.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16800..19442
                     /gene="alaS"
                     /locus_tag="IV038_000748"
     CDS             16800..19442
                     /gene="alaS"
                     /locus_tag="IV038_000748"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP8340989.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKNGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEMAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19642..20238
                     /locus_tag="IV038_000749"
     CDS             19642..20238
                     /locus_tag="IV038_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8340990.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20360..21508
                     /locus_tag="IV038_000750"
     CDS             20360..21508
                     /locus_tag="IV038_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8340991.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21546..21959)
                     /locus_tag="IV038_000751"
     CDS             complement(21546..21959)
                     /locus_tag="IV038_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP8340992.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22038..22469
                     /locus_tag="IV038_000752"
     CDS             22038..22469
                     /locus_tag="IV038_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP8340993.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22568..22801)
                     /locus_tag="IV038_000753"
     CDS             complement(22568..22801)
                     /locus_tag="IV038_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP8340994.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22984..24654
                     /locus_tag="IV038_000754"
     CDS             22984..24654
                     /locus_tag="IV038_000754"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP8340995.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24801..25187
                     /locus_tag="IV038_000755"
     CDS             24801..25187
                     /locus_tag="IV038_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8340996.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25174..25872
                     /locus_tag="IV038_000756"
     CDS             25174..25872
                     /locus_tag="IV038_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8340997.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25877..26341
                     /locus_tag="IV038_000757"
     CDS             25877..26341
                     /locus_tag="IV038_000757"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP8340998.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26338..27249
                     /locus_tag="IV038_000758"
     CDS             26338..27249
                     /locus_tag="IV038_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8340999.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27484..28023
                     /gene="pyrR"
                     /locus_tag="IV038_000759"
     CDS             27484..28023
                     /gene="pyrR"
                     /locus_tag="IV038_000759"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP8341000.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28037..29320
                     /locus_tag="IV038_000760"
     CDS             28037..29320
                     /locus_tag="IV038_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP8341001.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29333..30259
                     /locus_tag="IV038_000761"
     CDS             29333..30259
                     /locus_tag="IV038_000761"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP8341002.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30278..31558
                     /locus_tag="IV038_000762"
     CDS             30278..31558
                     /locus_tag="IV038_000762"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP8341003.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31563..32639
                     /gene="carA"
                     /locus_tag="IV038_000763"
     CDS             31563..32639
                     /gene="carA"
                     /locus_tag="IV038_000763"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP8341004.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32644..35829
                     /gene="carB"
                     /locus_tag="IV038_000764"
     CDS             32644..35829
                     /gene="carB"
                     /locus_tag="IV038_000764"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP8341005.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35883..36659
                     /locus_tag="IV038_000765"
     CDS             35883..36659
                     /locus_tag="IV038_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP8341006.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36656..37588
                     /locus_tag="IV038_000766"
     CDS             36656..37588
                     /locus_tag="IV038_000766"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP8341007.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37585..38301
                     /gene="pyrF"
                     /locus_tag="IV038_000767"
     CDS             37585..38301
                     /gene="pyrF"
                     /locus_tag="IV038_000767"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP8341008.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38308..38937
                     /locus_tag="IV038_000768"
     CDS             38308..38937
                     /locus_tag="IV038_000768"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP8341009.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            38925..39239
                     /locus_tag="IV038_000769"
     CDS             38925..39239
                     /locus_tag="IV038_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341010.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39277..39918
                     /locus_tag="IV038_000770"
     CDS             39277..39918
                     /locus_tag="IV038_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8341011.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39949..40653
                     /locus_tag="IV038_000771"
     CDS             39949..40653
                     /locus_tag="IV038_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP8341012.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40650..41552
                     /locus_tag="IV038_000772"
     CDS             40650..41552
                     /locus_tag="IV038_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8341013.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41710..43416)
                     /locus_tag="IV038_000773"
     CDS             complement(41710..43416)
                     /locus_tag="IV038_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP8341014.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13958 a         7684 c         9466 g        12460 t
ORIGIN      
        1 attataaaca agtaaatgga taaagtatat gtaatgataa aagaaaaatg gattttctaa
       61 tttttaagct aaatgttaga tcacacatgt atatgctgga tagtcttata aaaaatcatg
      121 taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac acaaaaaaga
      181 aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct gaggtgctgc
      241 tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca ctggaagaag
      301 caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg
      361 ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac tcagcagttg
      421 attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt gagcttccag
      481 gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca ttcgatctag
      541 ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg cttggcatct
      601 cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga taaacatcag
      661 aggctacgtg tgacgattct gaagtgacag gtgtcatttt tttattgtaa cctgtccaca
      721 cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac accccagaag
      781 aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc ttaccggctt
      841 gatataaccc agcgatttgc gcattcgtac cagttccttc agtgatcgta tcttttaaaa
      901 tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct ggctcataag
      961 tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg taataagtcc
     1021 ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct ccgtattttg
     1081 tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc gtactgtatt
     1141 cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca ttgaataatt
     1201 tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg gtgcccatat
     1261 attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac ggtgcgtcag
     1321 taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa tctgtgacag
     1381 gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg cctaaagtta
     1441 cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta ttggtgtcga
     1501 taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga tccgtgttga
     1561 cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt
     1621 ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct ttgacataat
     1681 tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca tccgttaccg
     1741 gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga agcatcgtat
     1801 acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga tcataggcgg
     1861 aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt
     1921 tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt gacatataca
     1981 ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc aatcttacag
     2041 ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg gtggagaaga
     2101 aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa
     2161 agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt ttataaaaac
     2221 ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa
     2281 ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt gtatatagtt
     2341 tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct tttgcgtaat
     2401 accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt attcctaaaa
     2461 ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa cctttgccag
     2521 aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag cttctagttt
     2581 gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata gaagtcaacc
     2641 gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc tagttcttcg
     2701 atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc
     2761 aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa caagacaaag
     2821 cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc atggaagttt
     2881 ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa gtaaaagcct
     2941 cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc cttgatcaca
     3001 gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg ctttttatgg
     3061 ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc ttcttcaaag
     3121 cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat tttttcgacg
     3181 ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat ctttaatcac
     3241 actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct gagatggata
     3301 agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt tttaaagaaa
     3361 atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat cagttagcag
     3421 aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc
     3481 aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt ttgtatcctt
     3541 tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac
     3601 aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct agtcagctaa
     3661 agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc tatgatgaag
     3721 aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa gcagatcatc
     3781 cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa taatcgatat
     3841 tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg ttattacgta
     3901 aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag acattttaca
     3961 acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg aattcttaga
     4021 caatatcatc aaagattatg aaacttacag caaagaactc cttgctttac aagaagaaaa
     4081 cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag ctcaaacacg
     4141 cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata tcttaaaacg
     4201 cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag caagtgcagt
     4261 caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct tagatgataa
     4321 tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg ggtaatcgcg
     4381 gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt
     4441 tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg aaaacaagct
     4501 aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc ggctggggaa
     4561 acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat ggtaggggcg
     4621 ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata gatggttacc
     4681 gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga aaaccgcaaa
     4741 catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa aaacatttga
     4801 aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact tttgtatcac
     4861 cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc tccttttttt
     4921 ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg caactgcggc
     4981 aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg aatgccaagt
     5041 tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag ctaatttatg
     5101 gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga
     5161 tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac tagatgcagc
     5221 ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg attgccaagc
     5281 gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt
     5341 accactgacg gaaactggcg cacattttca gttggaagta gctttgctga aagaccatgt
     5401 gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc gcttagaaaa
     5461 aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga caaattggcg
     5521 aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta ttgaagcagc
     5581 cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg aatcttggga
     5641 ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa aagcagatta
     5701 tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg aaattgccaa
     5761 agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac aagcagtcaa
     5821 agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt acggagaacg
     5881 tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg tattccgtcc
     5941 attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg aattttacgg
     6001 acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg atctattcca
     6061 gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt actaattgat
     6121 tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt tacaagaagt
     6181 aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata cacaaacagg
     6241 tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt acagcattta
     6301 tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt ctgaacatcc
     6361 ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag agtatgattt
     6421 ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt cttcagcaca
     6481 cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac acgcattgaa
     6541 tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg aaaagacgaa
     6601 ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc ttgataaggt
     6661 atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag aaaaagggac
     6721 tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga agagatttgt
     6781 agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag atgacactgt
     6841 ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac gatggaacga
     6901 aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg gtatgtatga
     6961 acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat caatgggtat
     7021 ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca gcttctggaa
     7081 aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata ttccttttga
     7141 caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg aggcagacag
     7201 tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga
     7261 aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag agtatctggg
     7321 aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt caggtgcaag
     7381 ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat tacttcattc
     7441 tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt ctccaatcaa
     7501 aaactggatg tcagagcata tccatcaata tggtgcatct agaaaaccaa atcaattaat
     7561 catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata tgaaagacct
     7621 gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct gaaattcatc
     7681 taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat ggataaatta
     7741 caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg acatgggagc
     7801 gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga accgcaggca
     7861 gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga taaagtcgtt
     7921 aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt gccaatagta
     7981 gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc taaaaatctc
     8041 tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga tcgtatcgaa
     8101 gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca tgggcacccg
     8161 atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc
     8221 acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact
     8281 gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa
     8341 ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg gatcaaaaat
     8401 aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt cggatcattt
     8461 tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg ccgttcagct
     8521 ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat attgtaactt
     8581 gtgattcgga attggcagga gaacaccgtt tattcggaag aataggactg tgacaagact
     8641 tttgtcacag tccttttttc gaattcaaca agtaaaactt ttaagaaata ttcagagagt
     8701 aaaattaaaa attgctgata aacgaagatg gaaattttat gtatttttat tggtctatgc
     8761 aataaataaa ttaaatattc agaatacaat tgattttaac gaaagggctt ctatcaatgg
     8821 ttgtgttgta tttatctgcc caggtagagt aaaatagatc attatctaaa aggtaattgt
     8881 tcatactata taagtcattt gaaagtaaaa agatactgta aataaaaaat gtggttaaat
     8941 aattctcata atcagtgtaa gcgttatcaa aaatatagca tacttgcttt gtagatgaat
     9001 gacacgatat atgtttggta tgttctgcaa gacagatgaa tatggaggtg gagacaatgt
     9061 cttccaaaga actaacaatg tatttagaga aagaattgga tcaactgaaa gaaaaggggt
     9121 tatacaatac gatcgacata ctagaaagtg aaaatggtgc ttgtatcatc gtagacggaa
     9181 aaaagatgat caatctagca tccaacaatt atcttggatt tgccaatcgc gaagaattga
     9241 aaaaagcatg tattgaagca accgaaactt atggtgtagg agcaggagca gtccgaacga
     9301 tcaatggttc gctgaaaatc catcagcaat tagaagaaaa aatcgcagaa ttcaaaggga
     9361 cagaagcagc aatcgccttt caatctggat tcaactgcaa tatgggcgct atctctgcag
     9421 taatgacaaa agaagatgct atcttatcgg atgaattaaa ccatgcatct attattgacg
     9481 gttgccgctt atccggagca aaaatcatcc gaattaaaca tcaagatatg aaagaccttg
     9541 agaaaaaagc aaaggaagcg atagaaagta agaaatataa aaaaatcatg tatatcacag
     9601 atggtgtttt ctccatggat ggagacattg cacgattgcc ggaaattatt ccgattgttg
     9661 aaaaatatgg actgatcacc tacgtcgatg atgcgcacgg ctcaggcgta actggaaaag
     9721 gtgcaggaac cgtcaagcat tttggattaa gcgacaaaat cgatatgcaa atgggcacgt
     9781 tatcgaaagc tataggtgtt gttgggggtt atgtagctgg atcgaaaaca ctaatcgact
     9841 ggttgaaagc tagaagtcgg ccattcctat tttctacttc gctaactcca ggtgcagctg
     9901 cagctgcatt ggcttcgatt actttgatgc aagaacatcc ggaacttgtc gaaaaagttt
     9961 gggaaaatgc taattatttt aaagaagaat tgaaaaaagt aggctataac atcggtatgt
    10021 ctgaaacacc tattactcca gtcattttgg gagacgaaaa agtgactcag actttctcta
    10081 agaagctgat cgaacatggg atttatgcta aaccaatcgt ttatccaact gtgcctcttg
    10141 ggacaggcag gatcagaaat atgccgactg cggaacatac gagggaaatg cttgatgaag
    10201 cagtagcagt ttatcaaaaa ataggaaaag aaatggaaat tatttaacta aatttagttt
    10261 tttcgtctat ttaaaattag agaacatgtt cacgcatata ttgttaatca tctgaagata
    10321 gtagtaaaaa agatcaggga ggatcatcat gaaaaaagta ttggtgacag gatgtttagg
    10381 acaaattggt tcagagttga tcacacggct acgtcaagaa ctaggcagtg aaaatgtcat
    10441 tgcgacagat atacgcatgc ctgaagtcat tgagaaaaac gatatctttg agatcctaga
    10501 tgtaatggat tacgaaaaaa tgcgcagcat agcagaacaa tatcaagtgg acacattgat
    10561 tcatttggct gctcttttgt cagctgttgc agaagcaaaa ccacaattag cctggaattt
    10621 gaacgtgact gggctggtca atgcattgga agtcgctaga gaattccagc tgaaattttt
    10681 tacgccaagt tcaatcggcg cgtttggtcc aaatactcca aaagatcata ccccacaaga
    10741 tactcttcag cgtcctacaa ccatgtatgg ggtaacgaag gtagctggag aactactatg
    10801 tgattattat tatgtgaaat atggcgtaga tactcgtggt gtccgtttcc ctggattgat
    10861 ttcttatgca accttgccag gaggcggtac gacagactac gcagtcgata tttattattc
    10921 agcaatcaaa gaaggaaagt acactagttt cattgatcaa ggtacatcaa tggatatgat
    10981 gtatatgcca gatgcgatcg atgccattat aaaactcatg aatgcagatc ctgatcgatt
    11041 ggttcatcga aatgccttca atatatcggc gatgtctttt gacccggaag agatcaaagc
    11101 atctattcaa aaaatcatac cagattttga aatggattat gatgtggatc ctgtacgtca
    11161 gtcaatagca gaatcttggc caaattcact agatgtttct tgtgcgcaag aggaatgggg
    11221 cttttcgccg aaatacgact tagatgtgat gacaaaggat atgttggaaa aactgaaaga
    11281 aaaattggta gagacggtat aaaacaagaa ccacatccaa aaagtttttt atacttttgg
    11341 gattggctat ggtatgaaag gggacaaggg atggaagaat atcaagccac gccagtaaaa
    11401 gaagtaaaaa tagaaaatga attaaaacgt acgatgggat tttttactgc tttatcgact
    11461 gttatgggca cagtgatcgg tgcaggggta ttttttaaag ccgcaagcgt agcagaagtt
    11521 acaggatccg ccagtttgca tatgttttct tggtttctcg gtggtatgat ttctgtctgt
    11581 gcaggactaa caggagctga actggcagct gctattccag aaacaggagg gatgattaaa
    11641 tatatcgaac ggatttacgg aaatacagca gcttttttat tgggctgggc gcaagtcgtg
    11701 atttattttc cagcaaatgt agcagccttg tcgatcattt tcggtacaca gttcgtcaat
    11761 ctatttggtc tttcccagtc aatgattgtt cccgtagctg ttacagctgc tgtatcgatc
    11821 ctgttgatca attttttagg ttctaaagct ggaggggctt ttcagtcgat tacgcttgtc
    11881 tgcaagctta ttcctttgtt tgtgatcgtc attttcggtt tgttccgaca agaaggagta
    11941 gacttccaac tgtttccgat ccaagcagga gaaaatcttt catttttctc agccttggga
    12001 gcgggcttgc tagcaacgat gttcgcatac gacggttgga tacacgtagg aaatatttcg
    12061 ggagaattga aaaagccggc gaaagatttg ccaaaagcaa tttctttagg aatcatcgga
    12121 atcatgatcg tttatttatt agtaaatgcg gtgttcttaa gaaccgcatc tatcgacgga
    12181 gtcgcaggaa acagcaatgc ggcaagtgac gttgcaaaaa tgatttttgg cggcttcggt
    12241 ggaagactgg tcacagttgg tatcttgatt tctgtttatg gaaccatcaa tggctatacc
    12301 cttactggaa tgcgtctccc ttatgttatg gcaaaagaaa acaacctgcc ttttagcaag
    12361 ctctttgcta aacttcatga taaaactaaa gttcctgtag ccgcaggtat cttagagcta
    12421 gtcatcgcta ttggcatgat gatgatcggt ggttttgata cactaacgga tatgctgatc
    12481 tttgtcattt ggatttttta tacgatggtc tttgttggtg tcattcttct tcgtaaaaaa
    12541 gaacctgatt tgtttcgacc atacaaagtt ccaatgtatc cgtttattcc actagtagca
    12601 atcattggag ggacttttat cgtatcaagc acgcttatca ctcagacctt tttagcttcg
    12661 atggggatag tattgacact tgctggtatc ccgatttatc tctatttaaa acgtagatag
    12721 ctaaaacatt cgaattttat tagaactgca cttctctgcc aaacaaaata ttttatttaa
    12781 atgaggagta cgtggaagaa atgtcaaaag tactcgttaa ttttgatcac gtaaatgtcg
    12841 ttttgtctac gatgaaatag caaagtaaaa gacagaaatc gggctcaacc gatttctgtc
    12901 ttttactttg atttgcgtaa ctgaactatt cggcgtacgc cgcgataaat atttaataaa
    12961 accaagcaac cagaaatgaa aatgggaagc caaatcagat agacagggta gccaagcact
    13021 ttagacaaag catgtggagt cattttatcc atttcaagaa gtgcttgctg ccaagtctga
    13081 agattgaaga ctagcatgaa acccgaaata atcaggagaa taaatcctaa ataatgaatt
    13141 gtagttcttt ttcttacata agcgatcgta gcataaagga acagtaaaaa aataggtacg
    13201 ccaaaaacaa agtacatatt cagcatctcg acaccccctt tgtgtttatc ttactttact
    13261 ttgaaagaaa tgagaaataa tagcctcgtt acttgcttga aacagcaaag gactgctaag
    13321 ataagagaga tacaaagaat caaggaggag tcatatggca agttatatcg aaccgattcg
    13381 ttttgcgctg attgtttttc catttttagc cttagcgatc tcatctgttt tttttattta
    13441 tgaatatcga aagtatggaa catttctagt aacaagggca gttattttgt attctttcgt
    13501 tttttattta ttgtgtgcgt attttttggt gatcttacca cttccggcaa aggagacggt
    13561 tgcccaaatg acagggccaa agatggagct gcatttattt gcttcatggc aacatttcag
    13621 agacaacacg gtattggtct tgtctgaacc tagtacctac ctgccggcaa tgaagcagag
    13681 tgtatttttg gaaccagctt ttaatgtttt attactcata ccatttggca tttatttaag
    13741 atattatttc cggctttctt ttatgaaaac gatgattgct agtttctgcc tttctctatt
    13801 ttttgaattg acgcagctaa gtggacttta ttttatctac ccaagaccat accgcttatt
    13861 tgatgtgaat gatttagtga caaatacatt gggtggaatc attggttttg tcgttacgcc
    13921 gttatttaca tttatgcttc ctacaagaag acgaatggat gaactttctt atgaaaaagg
    13981 aaagactgtc tcattgactc gacgcttggt cgcttatttg atcgactgga catttttatc
    14041 tcttattaat ttggtagcgg cgattttctt taaaattgcg ttcgattcag gggcagttac
    14101 ttctcatatc tggtggcttt tattagaagt aggggtatac tttatgattg taccgtttat
    14161 aaccaatgga tggacacttg gcaaaaaaat tgtgcgtatc cgtttggtgg aagaagaccg
    14221 cgaacgaatt tctttaaaag cattgatgat ccgctacggg tatctgtact ttctttttta
    14281 tggtttaagc tggttggggc agttgcttca gcaaatcatg aattcgacaa cgaatacggt
    14341 gttgcagatg agtgcagtat tcatttctct cggtgtcttg ttactacaag gtatttttat
    14401 tttaaatatc ttgctatcat ttttgagaaa aaacggattc tgttttatga aaaagctagt
    14461 cacacatata ctgttagcac gattggccaa acaaaggatg atgattctcg aaattagctg
    14521 agcaaaaacg ctttgtctgt gttataatgc caaagtcgtg ataatttaaa gagaagaggt
    14581 gcacgaatga ataattttga tatattccgc ttcaaaccgt ttatcaatga agcgttaacc
    14641 gcaaagggct tcaaacagcc cacagaggtc caggaacgtt tgattccttt aattgcaaaa
    14701 ggaaaaaata tcattgggca atctcaaact ggtacaggaa aaacacatac gtttttgttg
    14761 cctttgtttg aaaaaatcaa tgcagaaaaa gaggaagtac aagttgttat tacagctcca
    14821 agtagggaac tagctgcaca gatctatgaa gcagctacac aaatcgctaa attttctaca
    14881 ccggagatcc gtgtctcgaa ttttgtcggc ggtacagaca aacagcgtca attaaatcgt
    14941 ctgcatcacc aacagccaca ggttgtagta ggcacgcctg gtagaatatt agacatgatg
    15001 aatgaacaag cgctgaagat tcacaccgca tttgcatttg ttgtcgatga agcggatatg
    15061 acattggata tgggtttttt aaatgaagta gaccaaattg caagtcgatt accagaaaag
    15121 ctgcagtttt tagttttttc tgccactatt ccagaaaaac ttcgtccatt cttacgaaaa
    15181 tacatggaaa atccaatcat tgaagaaatc aaaccaaaag cagttatttc tgaagacatt
    15241 gaaaattggc tgatttcaac aaaaggtaaa gatgagaatc aaatgattta tcaactgctg
    15301 acaattggtc atccttattt agcaatcgta tttgccaata caaaacagca tgtggatgaa
    15361 attgccgatt acttgaaaaa ccaaggcttg aaagtagcaa agattcatgg agacatcacc
    15421 ccaagagaga gaaaacgagt gatgcgacaa gtacagaatc tggagtatca atatgttgta
    15481 gcgactgatt tagctgctag agggatcgat atcgaaggtg tctctcatgt tattaatgca
    15541 gaaattccag aagatctaga cttctttatc catcgtgtcg gacgtactgg acgtaatggc
    15601 ttaaaaggaa cggccattac gttatattcg ccaaatgatg agcaagccat tgatgcgata
    15661 gaaaaattgg gcgtctcatt tgaaccaaaa gaaatcaaaa acggggaaat cgtcaaaaca
    15721 tacgatcgta atagacgtgc caaacgagaa aaatcaaaag atgtattaga tcccacgctg
    15781 attggtcttg tgaagaagaa aaagaaaaag atcaagcctg gttataagaa aaaaatcgat
    15841 tgggcaattg cagaaaaaaa taaaaaagaa cgcaaaatcg aacgcagaca gcaaagccgt
    15901 actgctcgta aaaataagaa aaattcacat aaataaaaaa caagaggttg tgacaaaagg
    15961 cgtgtcacaa tctcttgttt ttaagctaga ctttttttca ttccgataca aagtacttca
    16021 tcttcaataa aaggttctgt gcaagtatga tacccttgtt tttcataaaa atctacagcc
    16081 gtcatttcag ctactacata actagtctga caaccttctg aaaccgcccg tttttcagca
    16141 gcttcaagca actgaccgcc taaccctttt cctcggtatt cttttgctac gcaaaatcgt
    16201 tccggcttga agcaagtagg tgttttcttt tcataacgca aagtagctac tggtcttttt
    16261 tgatcaagaa atagataata ggtttgttgc gggatatctt ttccgtcgaa ttctagttct
    16321 gcaggaatcc cttgttctaa tacaaaaact tgatatctca aaaagcagct agctgcgcgt
    16381 atccattcat ccgctccaac tatttttttc atcatattcc tccttgtcat gaatagcaga
    16441 gatgtattga ttcctttatt cttatcgtac caaaaatcgt ggagtatgac caaaaggaaa
    16501 tgaaaataaa aattgagata ttgacagtga tcaatagaat gcgtatacta attaagtacg
    16561 aaaaggatat gatttgtttt ttaagactca cagaaaattc tttcttggct gaaaaaagaa
    16621 tagtcactaa tcaaattgct ccctttttct attttggtag aaataccgac gcagctcagc
    16681 gttatcgagt ttaagaggta atgattacac atcattgcaa tcaaggtggt accgcgagtt
    16741 ttcgtccttg gctgtgatgg tgtttttttg tatttaattt atagaaagca ggtaatgaca
    16801 tgaaacaatt aagtagtgca caagttcgac aaatgtttct tgatttcttc caatctaaag
    16861 gacatacgat tgaacctagt gcgtctttga tccccgtgaa cgatccaacc ttattgtgga
    16921 tcaactctgg tgttgcaaca ttgaaaaaat attttgatgg ttcagttgtg ccagaaaatc
    16981 caagaatcac aaatgcccaa aaatctattc gaacaaatga tatcgaaaat gtcggtaaaa
    17041 cagcacggca ccatacgatg tttgagatgc tgggtaattt ttcgattggt gactatttca
    17101 aaaatgaagc catccactgg gcatgggaat ttttgactga tccaaaatgg atggcattcg
    17161 atccagaaaa actttatgta acagtttatc caaaagatac agaagcaaaa cgcatctggc
    17221 atgaagaagt tggcctgcca ctagatcaca ttattgatgt agaagacaac ttctgggata
    17281 tcggtgcagg cccatgtggt ccggatacgg aaattttcta tgaccgtgga gaagcttata
    17341 acgatttagc agaagacgat cctgaaaact atccaggagg agaaaatgaa cgttatttag
    17401 aaatctggaa cttagtattt tcagaattca accataagcc tgataacaca tttgaaccac
    17461 ttcctcataa aaacattgat acggggatgg gattggaacg catggtctca atcgttcaag
    17521 atgcgtcaac gaactttgag acagacttgt ttatgcctat tatccacgcc gtagaaaagc
    17581 taagcaaaca agtaaaaaat ggtgaaaacc cagtaacaga tatttcttac aaagtaatcg
    17641 cagatcatat tcgcgccctt tcatttgcta ttggcgacgg tgcattgcca tcgaatgaag
    17701 ggagagggta tgtcttacgc cgtttgcttc gtcgtgctgt catgcatggg aaaaaattag
    17761 gtattgaaca agcattctta tatcgtttag tccctgttgt tggggaaatc atggtaagtt
    17821 attatcctga agtacttcaa caaaaagaat ttattgaaaa agttatccgt acagaagaag
    17881 aacgtttcaa tgaaacaatc aatgaaggtt tggaaatctt aaatcaagtg atccaagaag
    17941 taaaaaacga aaacgggacg actctagcag gaaaagacat cttcaaacta tacgacacat
    18001 atggcttccc ggtagaatta acggaagaga tggcagaaga tgaaggactg aaagttgatc
    18061 atgctggttt tgaaaaagaa atggatgcac aacgcgaacg tgctcgctct gcccgcagta
    18121 ctgaaaaatc aatgggggta caatctgctt tattgacaga tatcaaagta gaaagtaaat
    18181 tcattggata cacagaacaa gaatcagata gtcaattatt ggtcatcgtt aaaggtgagt
    18241 ctcttttgaa tgaagtcgca gaaggtgaag ctcaaatgat ctttgatcaa acacctttct
    18301 atgcagaaat gggtgggcaa gtagcggatc aaggatggat catgaatgaa gtaggcgaaa
    18361 tcgtagcagt tgttacagat gttcaaaaag cgcctaatgg tcaatttatg cacaccgtag
    18421 aagtaaaagc accaatgagt gaaggacaaa cctatcatct attgatcgat caatcgatgc
    18481 gcaatcggat catcaaaaac catacagcaa cccatctact gcacaaggca ttgaaagaag
    18541 tacttggcga gcatgcaaat caagctggtt ctttagtcgc tcctggacat ttacgattcg
    18601 actttaccca ctttggtcaa atcacttcag atgaattagc tgaaatggaa agaatcgtca
    18661 acgaaaaaat ctgggaagct atcccagttg aaacaattga aacagatatc gatacagcca
    18721 aaaacatggg tgcaatggct ttgtttggtg aaaaatacgg acatgacgtt cgagtagtaa
    18781 atattggcgg ctggtcaatc gaactgtgcg gaggcacaca tgtagcaaat acagaaaaca
    18841 tcggaatctt caaaatcgtt tctgaatcag gaattggtgc aggcgtgcga cgaatcgagg
    18901 ctgtaacaag taaagaagcg tacgaactac ttgaagaaga agaaaaacaa ctaaaacaag
    18961 tagcaacaat tgttaaatca cctcaattga aagaagttgt ttcaaaagcg gaacaattgc
    19021 aacagcaagt aagagagcta caaaaggaaa atgaacaatt agctagcaag ttagcgaatc
    19081 aacaagctgg tgatgtattc aaagatccag aaacgatcaa tggaaaaaca atcattgcag
    19141 cacaagtaaa agtgaaagat atgaatcagc tgcgtcaatt agctgaccaa tggaaacaaa
    19201 aaaatatttc agatattctt gttttagcaa acgaacaaga aggaaaagtc agtctgctag
    19261 cggcaatgac gaaagaatca aatgaagctg gactaaaagc tggcgacttg attaaagcaa
    19321 tcgcaccgaa agtaggcggt ggcggtggtg gacgtccaga catggcacaa gctggcggga
    19381 aaaatcctgc tggtattcca gatgcactta aagctgcgaa agaatgggtt gcgcaacaat
    19441 agggcgttta cgtttgacag aaaaacaaaa gttcaaacat tgaacgaaac atgattatgg
    19501 gggcgaagac aaacaagtct tgccctcata cttttttcaa ttggatggcg catctaatca
    19561 aatggtcgga ttcggatggt ctttagttga cgaaagtgac agaaattttt acgattagta
    19621 taaataaaaa ggggtgaaaa aatgaatagt agaaataaga cctttcgact agtcttacgt
    19681 gcgatcttgt tagcaatcat catcgtacaa gcgatggttc cttggctggg gtttattcca
    19741 ttaggattca ttagtttaac aattatccat atcacagtaa ttattgctgc agtagtatta
    19801 ggcccaaaag atggcatggt gattggcttg ttttggggaa tcgctacaat tgttcgtgcc
    19861 tatgccatgc caacaacgcc atttgataca ttagtgttca cgaatccgat tatctcggta
    19921 gttcccagag tacttgttgg cttagtagca ggcttggttt tccactggat ttatcaaaaa
    19981 aataaatcaa taacgatcag ctcagccttt gcaggggtac tgggttcatt ggtcaataca
    20041 gtcttagtgt taggattcat gggattattt tatacaggtg caacggcaga tgcctatggt
    20101 gtagactctg ctcttttatt taaaacctta gcaggggttg cagcaatcaa tgggatacca
    20161 gaagcaatag gtgcgggaat cataacacca ttactagcaa aagcgttatt tgcagcaaca
    20221 ccgttgaaac cagaataatg accatctttt aatagtaata ggacgcacga tagatagaaa
    20281 aaggaagaga ggagaggaaa acaatcgtcc tctccatctt tttacctgag caacaagcaa
    20341 actagaaggg gtaaaaaact tggctaagaa acaagaagaa aaaaagaaac aaataccgca
    20401 acagcggaca aaaaaaggag tactagcggg tatcttagga ttggttacga atctgatctt
    20461 gtttgtctct aagctaatga tcgggctttt ttcaggaagt gtctccatta tggctgatgc
    20521 agtgaacagt ctatcagata ctgcttcatc catattgact ttgatcggat ttaaaattgc
    20581 tgcaaagcca gcagatcagg aacatcctta tggacacgaa cgcttcgaat atatcagtgg
    20641 gttatttgtt tcgatcatca ttacttatgt aggatttcaa tttttagagt cttctgctga
    20701 aaaaatcatc catccagaaa atgtctcttt aacacccatc gtcttttttg tcctaatcat
    20761 ttccatcttt ttgaaatttg cacaaggcaa aatgtatcag gttattgcgc aatccattga
    20821 ttctgaaaca ttgagagcta cagcaacaga tagttataat gatgtcttta ctacactggc
    20881 tgtattgctt tcagcagcaa tcgaatgggt aactggatgg cgtattgatg gatatatagg
    20941 ttttgcttta gcattatata tcctttatag tggattgacg atgcttcgaa attttgtata
    21001 cgaactatta ggaagtcgcc caacgccagc agagattgaa gaaatggaag agcatttaaa
    21061 tgcttgcgat gatatacttg gctatcatga tttattggtg cataactatg gacccaataa
    21121 acgctttgca tcagtccaca tcgaagtaga tgaatcaatg aatttgaatg tggctcataa
    21181 gattattgat ctgatagaaa aagactttaa aaagtctttg gatgtggaac ttgtctgtca
    21241 cttggatcca atgcctgttg ataacgaaaa atactataat atcttgcggc agttaaagca
    21301 aattcttctt gaaacaggta cagggcttaa tattcatgat tttagagtaa ttcatcaagg
    21361 aaaaacactg caatttgatg tagtggtccc tgagaaattc cagtttgttg atacagaact
    21421 agaaagaatc ttacgtagtc aaatagaaga aaaaatcgga gattaccaat tggaaattac
    21481 gtttgatcac aattatcttt tatattaaat gagaaactat aaaaagactc cttaaagttt
    21541 ttaatttaaa gagtcttttt ttcttttaat tgtttatgga gagcctgacg tgcaagatta
    21601 tctgctcctt tatttttgtt ttcgggcaac cattgcagga tcaataaaga aaattcggat
    21661 agaagttctt ggattctttg aaggtaggga aggaagagct cgttcttcgt tgcgtttttg
    21721 tcgaccgtag aaactagtgt tttgctgtct gaaaaacaga aaatcgtttc attctgtaag
    21781 tcgtttttct ttagaaattc taaagttttt aaaaagactt cgaactctgc ttgatggttt
    21841 gtccctatag aaagcgggat agatagctgt tcttgtttac cttgatagac aatcaagatt
    21901 ccacctccac taggaccagg gttgcctttt gtagaggcat caatgtatac ttttaacata
    21961 aaaagagatt ctcctttttg ttcaaactaa tgttatggta agattatatc attactgaat
    22021 cggtgagagg ttagagaatg aaaaaaaata ttcgatggca gcctgaactg gcacatacga
    22081 tcatctattg gtcattgacc ttgatcattc tgttttttag tctgattctt tctttagaaa
    22141 acacaggtcc ttattggaaa agcaattttg ttttaggtat ttttcttgtt tttctatttc
    22201 taggtttgcg gcgttctttt attttaaaat caaactctgt gattatacgt tatgctgctt
    22261 tttggaaaga ccgagaaata tctgtcggac agattcgatc tttgacgcca actaatactg
    22321 gggtgcaaat gatgttgcaa gatgagaaag ccccatctgt tttcatgatg cgaaaaaaag
    22381 atcaagtagt cctttttgat cattttgaaa aaaacggacc caaatcagaa tcaattcctt
    22441 atccttatac gaaaaaaaga aaaagctaat tttaaaaatc agaacctaaa aaaacgaatt
    22501 tttacaggaa aagaaaagag tgagacgtac ggttcatccc gtttagcgtc tcactctatt
    22561 tgtttatcta ttcatgcata ctagtatcgg tagtctgaac gttgcctttc ttgacatggg
    22621 cagcttgtgt tcctcgattc ccttcgatga tctcaaattc gaccacttga ttttcttcca
    22681 aagttttgaa tccctcttct tcaatcgcag taaaatgtac aaaaatttct tctgtgtcat
    22741 cataactgat aaagccgtat ccttttttgt tgtcaaacca ttttacaatt cctgtggtca
    22801 aagcactcac gacctttcta aagtctgatc atagccttca ttataaagag tatttataag
    22861 tacgtcaaat aaattaaaaa cctgtaattt tatgtttttt ttctgaataa aaaaaggtat
    22921 aattggcata tcttaacaaa gaagatattt tgttgaataa agttcgtgaa aaggggtaat
    22981 caaatgaaga atgatctgca aatttcacaa gaaacagagc tagaaccgat tgttgaagtg
    23041 gctagtaaat tagatcttaa ggaagacgat ttggaactgt atgggaaata taaggcgaaa
    23101 atcaattttt ctggaatcaa tcgatcaaag gataacgctg aggggcattt gatcttagtt
    23161 acttccatca atccaacacc agctggcgaa ggaaagtcaa cgatcactgt agggttagga
    23221 gacgcgctca ataaaatcaa taaaaaatct gttattgcat tgcgagaacc ctcacttggt
    23281 cctgtgatgg gaatcaaagg tggtgctgca ggcggaggat atgcacaggt attgcctatg
    23341 gaagatatca acttgcattt tactggtgac atgcatgcta tcacaacagc taataatgca
    23401 ttatctgctc ttttagataa tcatattcat caaggaaatg aattgaacat cgatgcccgc
    23461 cgagtgattt ggaaacgggt agtcgatctc aatgatcgtg aactgcgtaa agtaattgtt
    23521 ggcttgggtg ggccaatcca aggtgttcca agagaagatg ggtttgatat cacagttgcc
    23581 agcgaaatca tggctatctt gtgtcttgcg gcagaccttg aagatttaaa ggctcgtctt
    23641 gcaaggatcg ttgtcggcta tacatttgac cgtcagcctg tgactgctgg agacttgaaa
    23701 gcagaaggcg ctctggcatt actgctgaaa gatgcaatca agccgaatct tgtacaaaca
    23761 atttacggaa caccagcttt tgtccacggg ggaccgtttg ccaatatcgc tcatggctgc
    23821 aatagtattt tagctaccaa gacagcattg agattagcag attacgttgt cacagaggca
    23881 ggatttggtg cagatcttgg cggagagaaa tttttagata tcaaagtacc taacttgaaa
    23941 aaagcgccgg atgctgtggt gattgtcgcc actgtccgtg cattgaaaat gcatggcgga
    24001 atgaaaaaag atgaacttaa aaatgagaat cttgatgctt tgaaagtagg ttttgcaaat
    24061 ttaaagcgcc atatacgtaa tatggaacaa tatcagctac ctgtgatcgt cgcaatcaat
    24121 gaattcgtaa cagatacaga caatgaactc acactgttgg agcacttgtg tgaaaatcag
    24181 gggattcttg ccaagagagc gagtgtatgg gcagatggag cagaaggcgg cgttgattta
    24241 gcaaaagctg tagtacgatt gattgataga aaagaagcta attacaagcc attataccgc
    24301 ctagaagaaa cgatacaaga aaagacagaa acaatcgtca agaaaattta cggaggaaat
    24361 ggtgttgttt tcagcaaaaa agcgcaaaaa caaatagaag agtttacgaa gaatggatgg
    24421 gacaaactgc cgatttgtat ggcaaaaaca cagtattcat tttctgatga tccttcactg
    24481 ctaggtgcac cggaagattt caccatcacg atccgcgaaa tcatcccaaa attaggggca
    24541 ggatttttag tagccttgac tggagatgtt atgacgatgc ctggtttgcc taaaaaacct
    24601 gctgcattga atatggacgt gacaaatgac ggagaagtgt tgggattgtt ttaagcttga
    24661 ttataatcta ccatcgaagg agctgtattt tgactacagc ttcttttttt agacgatagg
    24721 gaattaattt tgtttacact aggatcattc tttttcttta ttgtattttg agctatacta
    24781 aagcataata ggagggtatg atgaaaacga catttataag aaaaaccggt ttttacggta
    24841 tgggtagctc gattcagcta agaatcaaca atgaaaaacg cagtgttctc agtcatgaac
    24901 aaaagataac gcttgatctg gaagtgccct ttacgatgca ggtcaccttt tattggctaa
    24961 aaagccccgt ttatacagtg aaagaagcaa agcctgctta tgtgatcaca atgaattact
    25021 tgattcttca aatctatccg tttttatttc tagcaactgg gttgggggcc gttttcctac
    25081 aaaatttatt tttcagtttt tttgcagtta ttgtgttgat tgcgttcttt ttctatataa
    25141 aaaaccgggc atatttgatc aaggaggaaa attatggcaa actctgatct ttttttaatc
    25201 agtttcaatc gaatcgaaaa atggatgaga gacgagatgg gaaatgcacg aaatatggga
    25261 tttaccgaac ttgtcagacg cctagctcaa agaaaacaat taatgatcag aaaatacgaa
    25321 gatgatcttt tacagcttgc tcagttaagg aatgcgatcg tccatgatcg gatcgcagtc
    25381 gattttgtca ttgcagagcc aaatgattgg acagtcaagc gaatccagct gatcgaacaa
    25441 gaactgattc agcctgaaac cgtgctgcct cgatttgcaa aacgtgtaac aggttttgaa
    25501 caagatcttc cgttacttga actgttaaag atagtagcag aaaaaaggta ttcacaattt
    25561 ccgttataca ataaaggaaa atttgtagca ttgattactt tacgtaatat cgggttttgg
    25621 ctagcaaaag aaagccaaaa aggtccggtt gatttaacaa ataaaagagc gttggatttg
    25681 atcatccaaa acggaaagta cacaaattat cattttgttc cagctaaaac acatatttac
    25741 gaggttgaag cgatgtttcg tgagcaaggg actttggaag ccgtacttat cactaaagat
    25801 gggaatcccg atggtaatct gctcgggatc gtacgacctc gtgatattta taaacaaata
    25861 gaaaaggatt gattttttgt taattattta ttggatattc agcgctttgc tagtaggagt
    25921 ggatcagttg gtcaaatggt ggatcactga taatctagcg cttggagaga cgaaaaatct
    25981 tataccaggt attttgtcac tgaatcatat tcgaaacact ggagccgcat ggagtatgct
    26041 agaagggaag atgtggttct ttactatagt gacactcatt gcagtcgttg tgattctcac
    26101 attgatgatt aaaaatcgtg aaaaaggtaa tcgctggttc atgattggat tgagtttgat
    26161 tctagctggt gcgattggca attttatcga ccgtattcgt cttggctatg tagtagatat
    26221 gttccagaca gattttatga gctttccgat ttttaacgta gcagatgtga cacttgtatg
    26281 tggagtgatt tgtatgttaa tttatattat tttagatgaa aaggatcaaa gaaaaaaatg
    26341 acagaactta aaacgacgat tcaccatgaa tctggtagga tcgataaagt attgactaat
    26401 ttattcaccg attacagtcg ttcgcaaatt cagctgtggc tgaaaaatgg agcggtttct
    26461 gtcaatggac agccagtcaa agcaaattac aaagtaaaac aaggagacga gattactgtc
    26521 ttaatccccg aaccagaaac attagatgtg gtcccagaaa atatcccgtt gacgatcgtt
    26581 tacgaagatg aagatgtagc ggtgataaac aaacctcaag gaatggtcgt tcatccttca
    26641 gcaggtcata cgcatggcac aatggtcaat gcgctgatgt accatatcaa ggatctttct
    26701 acgatcaacg gcgttattcg tccaggtatt gttcaccgaa tcgacaaaga tacttccgga
    26761 ctgctaatgg ttgccaaaaa tgaccaggca cacgaatcat tagctcagca attaaaagat
    26821 aagacctctt tgcgcaaata tgttgcttta gtccatggag agattcctca cgaaaaagga
    26881 accatcaatg cgccaatcgg ccgttcgaag gaaaaccgga aaatgcaggc gataagagaa
    26941 gatgggaagt cggctgtcac acattttact gtattggaac gtttcgaagg atttactctg
    27001 gttgaattaa aactagaaac cggaagaaca caccagatcc gtgtgcatat gaagtatatc
    27061 ggttatccgt tagctggaga tccagtatac ggaccgaaga aaactttgaa aggcaatggt
    27121 cagtttctcc atgcgcaaat gttaggtttt acgcacccga ctacaaacga aaagatgatc
    27181 tttgaagcac ctcttccaga agtttttgaa aaaactttag aaaaattaag aaaaaacatt
    27241 gcattttaat ttagataacg gtatagtaat taacaacgaa agcataaaac ttaatagaat
    27301 tcctttaagt ttagtcccgt gaggctaaga agggtgggag caaggatacg tatggtgcgc
    27361 cataagtaga ttgctcattg acatagtata gctatcagtg tatcagtata cgctcagtct
    27421 aaacattcct cctgtcctct tagggcagga gtttttttat gcaaaaaagt agggggaatg
    27481 aaaatgcaag caaaagaagt agtagatcag gtaaccatga agcgagcatt gactcgaatt
    27541 acttatgaga ttatcgagag aaaccatagt atccaagata tcgtattggt aggtatcaaa
    27601 acacgaggaa tctacattgc atcaagaatt gccgaacgcc tgaagcagct agaagatatc
    27661 gatattcctg taggagaact ggatatcact ttataccgag atgacaaaaa agaaaatcca
    27721 gaagaaccgg aacttcattc ttctgacatc ccagtttcct tagaaggaaa agaagttatt
    27781 ttgattgatg atgttttgta tacaggacga acgattcgag cagcaatgga cgcagtaatg
    27841 gattttggca gaccaagaaa aatttcatta gcagtcctgg tcgatcgtgg gcatcgagaa
    27901 ttgccaatcc gtgcagatta tgttgggaag aacattccaa catctcgggc ggaagagatt
    27961 ttagtagaga tgcaagagct tgatggacaa gatcgcatca tgattttgaa agaggaagat
    28021 tagaggagag agaaacatgt cggagaaaaa aggatttcat aatgatgacg tggtgttaga
    28081 tatccacgat cgcccaaccc caggacattg ggtaggatta agtttacaac acttgttcac
    28141 gatgttcgga gcaaccgtgt tagtccccat cttagtaggt atcgatccag gtattgcgct
    28201 agtcagttca ggactaggta cattggtgta cttatttact actaaaggaa agatacctgc
    28261 gtacttagga agcagcttcg ctttcattgc agcgatgcag cttttgatga aaacagatgg
    28321 ttatccagcg attgcacaag gagcagtcac aactggatta gtctatttga ttgttgcgct
    28381 gatcatcaaa aagatcggtt cagcatggct ggataaaatc ttgcctccaa tcgtcgtcgg
    28441 tccagtcatc atggttatcg ggttgggact tgcggcaaat gcagcaacaa atgctatgta
    28501 taatcagggc acgtatgatt tcaagtatat tgctgtagcg ctgatcacat taggattaac
    28561 aattttcttc aacatgtggc taagaggttt tctcggattg attccgattt tactaggtat
    28621 tattagcgga tacatcgtag ccttgctgtt tggaatcgtg gatactaagc caatcgtaga
    28681 cgctgcctgg tttgctgttc ctaactttga gattcccttt gtacaatatg aaccaaagct
    28741 atatcttgga gcaatcacaa caatggcacc catcgctttt gtgacgatga cagagcatat
    28801 cggccatctt atggtattga ataaactaac taaacgtaat ttctttgaag aaccgggtct
    28861 tcataagacg ttgacaggtg atggattagc acaaatcgtg gcagcctttg tcggaggtcc
    28921 tcctgtaaca agttacggtg aaaacattgg tgttctggct atcacacgtg tccatagcgt
    28981 gtttgtgatc ggtggagctg cagtatttgc agtcatttta ggattcgtag gaaaattaag
    29041 cgctttgatt ttaagtatcc ctggaccagt catctcagga atcagtttta tcttatttgg
    29101 agtaattgcc gcaagtggaa tgaagatttt aatcgagaac aaaatcgatt ttgataaaaa
    29161 gaaaaatctg ttgattgctt cagttatctt agtagtagga attggcggac tcgtctttga
    29221 agtaggcaca tttacattgt cggcaatggc attagcaact gtacttggca tcattttaaa
    29281 tcttatctta ccagaaactt cacgaagcga agaaaactaa ggaggaaaca ttatgatcat
    29341 caaatcagaa cgtatcagct taaagcattt attgaccgtt gaggcactta ctgatcaaga
    29401 agttatgggc ttgatccgca gagggcagga attcaagcaa ggtgctaatt ggactccgca
    29461 aaagcgccag tactttgcta caaacctctt tttcgaaaat agtactcgca cacataaaag
    29521 cttcgatgta gcggagaaaa aactagggct agaagtgatt gaatttgaag caagcacaag
    29581 ctctgtccaa aaaggggaaa cactatacga tacagtactg acgatgtcag cattaggcgt
    29641 agacgtagcg gttatccgtc atggtgatga gaactattat gacgaactga tacaaagtaa
    29701 aacaatccag tgttcgatta tcaatggagg agacggaagc ggacagcatc caacccaatg
    29761 cttgcttgat ttgatgacca tttatgaaga atttggtcat tttgaaggtc tgaaagttgc
    29821 catcgtaggt gatatcactc actctcgcgt agctaaatcc aatatgcaga tgttaaaacg
    29881 tctaggcgca caagtattct tctcaggtcc tagagaatgg tacgacgatg aattcgaagt
    29941 gtacggacat tatatgccat tggatgaact actagatcaa gttgatgtca tgatgatgct
    30001 tcgggtgcaa cacgaacgcc atgatggaaa agaaagtttc tcaaaagaag gctaccatca
    30061 ggaacatggg ttaacagttg agcgtgcgaa gaagttgcaa aaacatgcaa tcattatgca
    30121 cccagcacca gttaaccgtg atgtagaatt agctgattca ttagtggaag gattacagtc
    30181 gcggatcgtt cagcaaatga gcaatggagt attcgtcaga atggcaatct tagaagcagt
    30241 gctaaatgga aaagcgtaat tataggagga tgaaaaaatg aaaacactca ttaaaaacgg
    30301 tcagatcaat acacgaaaaa atatgacgac accagcagaa atttggatag aagatgggcg
    30361 aatcaaagca atcggtacag gattctcaga agccgaattt gatgaagtgt ttgatgcaaa
    30421 gggacaattg atcacacctg gtttagtgga cgtccatgtt catttgagag agccaggatt
    30481 tacatacaag gagacgatcg aagctgggac tcgctcagct gctcgtggcg gtttcactac
    30541 tgtatgcgcg atgccgaatt taaatcctgt acctgatacg gcagaaaagc tgagacaagt
    30601 atatgacatc attagaaaag atgcagttgt gaaggtattg cagtatgcgc caatcacaga
    30661 aaatctgcga agtgagaaat tagttgatca agaagcactg attgaagaag gtgcttttgc
    30721 tttcacaaat gacggtgtag gtgtccaaac agctgggacg atgtatctgg ctatgaaaga
    30781 agctgcaaaa aacaataaag ccctagtagc ccatacagaa gatgaatctt tattatttgg
    30841 cggcgttatg cacgctggca agaaggcaga agaacttggt cttcccggga tcttaagcgt
    30901 aaccgagtct tctcaaattg ctagagatct gttgctagct gaagcaactg gtgttcatta
    30961 ccatgtttgc catgtttcta ctaaagaaag tgtccgtgta atcagagacg caaaaaaagc
    31021 aggaatccac gtaacagcag aagtttcacc ccatcatctt attttgatcg acgaagatat
    31081 tcccgaagat ttcggctttt ggaaaatgaa cccgccgcta agaggacggg aggacagaga
    31141 agctttgatc gaaggcttgc ttgacggtac gatcgattgc atcgctacag accatgcccc
    31201 ccatggatta gaagaaaaaa gccaaagttt catgaaatca ccatttggta tcgtaggaag
    31261 cgaaacagcg tttcaactga tctatactca ttttgtagaa actggtcgat tcacgttgga
    31321 acaagtgatc aattggttgg cagttaaacc ggctgagatt tttggtttga atgcaggaac
    31381 gctaacggtt ggtgcccccg ctgatgtagc agtttttgat attacacaaa cttgcacgat
    31441 cgataaagaa gactttttat caaaagggga gaatacacct tttatcggct ggaaagtaaa
    31501 aggcgaaaca caaatgactt ttgtcaacgg aaaacttgtg tggcagaagg gagaataaaa
    31561 gtatggaacg tttgcttata ttagaagatg gaacagtttt cgaaggaaag gcttttggtg
    31621 cagatgctag tgtcttcgga gaaatcgttt ttacaacaag tatgactggt tatcaagaaa
    31681 ctatcactga tcaaagtttt aacggtcaaa tcattacatt tacctatcct atggtgggta
    31741 attatggggt aaatagagat gattatgaat cgatttctcc aacatgcaaa ggagtagtag
    31801 taaaagaaca tgcacgtgta gcttcaaact ggcgaaacca aatgacgcta gattcatttt
    31861 tgaaacaaaa aggaattcct ggaatatcag gtatcgatac gcgcgccctt actcgcaaaa
    31921 tccgtgaagt cggaacgatg aaagctagta tagttgatgc tggagattcc ttcgaacatg
    31981 catttgatca gctgaaagca accgttctag caaccaatca agtgcagcaa gtctcaacta
    32041 acaaacctta tccaagtcca ggaacaggtc ggaaagtcat tgtcattgac ttcggcttga
    32101 aacatagtat tttacgagaa ttgagtaaaa gaaactgtaa cctcacagta cttccctata
    32161 acacagatgc cgagacgatt ttagcgctat caccagacgg agtcatgctg acgaatggac
    32221 ctggtgatcc aaaagatgtg ccagaagcaa tcgaaatgat caaaaaaatc caaggaaaag
    32281 taccgatttt tggtatttgt ctcggtcacc agttatttgc tttagccaac ggtgcagata
    32341 cctataaaat gaaattcgga catcgaggat taaatcaccc ggtacgagaa gtagcgaccg
    32401 gaagaattga ttttacttct caaaatcatg gctatgcagt cgatgaagcc acgattgatc
    32461 cagaaaaact catcgtaact catgtagaag taaatgacgg aacagtagaa ggagtcaaac
    32521 acagagatta tcccgctttc agtgtgcagt ttcatccaga tgctgcccct ggtccgcatg
    32581 atgcactaca tttatttgat gaatttatgg aaatgatgga cgcaggaaag gagcagtaaa
    32641 cagatgccga aacgtacaga tatcaagaaa attatggtga ttggatctgg tccgatcatc
    32701 attggtcaag ccgcagaatt tgactacgca ggaacacaag cctgccttgc cttaaaagaa
    32761 gaaggatatg aagtggtttt agtaaattct aatcctgcga cgatcatgac ggataaagaa
    32821 gtggcagatc aagtatatat cgaaccgatc acacttgaat ttgtatcaag gattcttcga
    32881 aaagagcgtc cagatgctct ccttccaact ttaggcggac agacaggcct caatatggca
    32941 atggagctgg cagcatcagg tattttagaa gaattgaaca tagaacttct aggaacaaag
    33001 ctttctgcga tcgatcaagc agaagatcgt gatctattca aacagttgat gaaagaattg
    33061 aatcagccaa tccccgagtc tgaaatcgtc aatacagtag aagaagctgt agattttgca
    33121 aataaaatcg gctatcctgt catcgttcgt ccagccttta ctttaggcgg aactggtgga
    33181 ggtatgtgtg aaaatgaaga agaattacgt atcgtatcag aaaatggatt aagtctttcc
    33241 cccgctaccc aatgtttgat tgaacgaagt attgcaggtt ttaaagaaat tgaatatgaa
    33301 gtgatgagag attcggctga taatgcaatc gttgtctgca atatggaaaa ttttgacccg
    33361 gtgggtatcc atacaggtga ttctatcgta tttgccccta gtcaaacatt gtctgaccat
    33421 gaatatcaaa tgcttcgtga tgcttctctt tcgattattc gagcacttaa gatcgaggga
    33481 ggctgtaatg tccaactcgc tcttgatcca catagtttta attattatgt gattgaagtc
    33541 aatccgcgtg tttctcgttc atctgcgtta gcaagtaagg cgactggcta tccgatcgct
    33601 aagctagctg ctaaaatagc gcttggatta acattagacg aaatgaaaaa tcctgttaca
    33661 ggaacgactt ttgcagaatt tgaacctgca ttagattatg ttgtagctaa aattcctcgc
    33721 tggccgtttg ataagtttga aaacggagaa cggcttttag gcacgcagat gaaagcaact
    33781 ggtgaagtga tggctatcgg tcggaatatc gaagagtctt tattgaaagc tgtgcgttct
    33841 ttagaaatcg gtacacacca tatcgaactt cctgagttgg cagacatcac ggacgatgac
    33901 ctaatccaaa aagtggttcg agcacaagat gatcgcctgt tttatgtagc tgaagctatt
    33961 agaagagggt acaccataga agaattagcg gatttgacaa aaatcgattt attcttttta
    34021 gataaactgt tgcatatcgt agaaatcgaa cagcagttga aacagcagcc gttggatgac
    34081 aagatattga aaacagcgaa acaaaacggt tttacagaca aaaaagtggc ggagctgtgg
    34141 gaaataaacg aagaagccgt tcgccagatg agaaaagaaa atgatttgat tcctgtatat
    34201 aagatggttg acacttgtgc agcggaattc gaatcaaaaa caccttattt ttatagttcc
    34261 tatgaatatg aaaatgaaag ttcacgatca gaaaagccct caattctagt actgggatct
    34321 ggtcctatcc gtatcggaca aggggttgaa tttgactatg cgactgtgca ttctgtcaaa
    34381 gcaatccagc aggcaggata cgaagcaatc atcatgaaca gcaatcctga aacagtttcg
    34441 actgactttt ctatttcgga taaattatat tttgagccat tgaccttcga agatgtgatg
    34501 aatgtgatcg agctagaaca gccgatagga gtaatcgttc aatttggtgg tcagacagca
    34561 atcaacttag ccgaaccttt gactgctgct ggtgtaaaaa tcttagggac aacgatcgaa
    34621 gacttggatc gtgcggaaaa tcgtgattta ttcgagcaag cgctgaaaga gttggatatt
    34681 cctcaacctt taggggatac tgctacaagt aaagaagaag ccgtggttat cgcttcgaga
    34741 attggttatc cagttcttgt tcgtccaagc tatgtattag gcggacgtgc gatggaaatc
    34801 gttgaaaacc agcgagattt agaagattac atggaacatg ctgtgaaagc atctccagaa
    34861 catccagtac tagtggaccg ttacttgctt ggtggtgaat gcgaagtgga tgcgatttgt
    34921 gacggtgaaa cagtactcat cccagggatc atggagcata ttgagcgtgc tggtgtccat
    34981 tcaggggatt caatggctgt gtatccacct caaacattat ctgaatcgat caagaaaacg
    35041 atcgaagatt acacgatccg cttagcatta ggtttgaatt gtgtcgggat gatgaatatc
    35101 cagtttgtca tccatgaaaa tgaggtttat gtgatcgaag tcaatcctcg agcaagtcgg
    35161 actgttccat tcttaagtaa ggtgacagga attccaatgg cacaaattgc gacgaaagct
    35221 attttgggag agaaactaaa gacacttggt tatgaaaacg gactttatcc agaaacaaaa
    35281 gaagttcatg ttaaagcacc tgtcttttct tttacgaaat tgcagaaagt tgatacttac
    35341 ttagggcctg aaatgaaatc gactggagaa gtaatgggat ctgacagaca attagacaaa
    35401 gctttgtaca aggcattcga agcttctgga ttacacttgc ctgaatatgg agccgtatta
    35461 tttacgattg cggacgaaac aaaagaagaa gcactagaat tagctaaacg ttttacagaa
    35521 attggctata gtctgatagg aacgaaacat acagctgctt atttcgaaaa aaatggtctg
    35581 gtcgttgaaa cagtagcgaa aatttctgag gaagcagcag aaaaaaatgt agtcgactta
    35641 ataagagatg gtaaaacaca agtggttgtc aatacgattg ataaagatcg gggaaatgct
    35701 tctaaagacg gtttcatcat ccgaagagaa gcagttgaac atggtattcc attgtttact
    35761 tctttagaca cagcagatgc gatcgttcga gtgatggaat cccgtgcttt ttcaactaac
    35821 cctatttaac ggcgagcaca tgttaagaca aaaagacgaa aaaccgttgc aggaggggac
    35881 agatgaaaca agaaatgatg gaaatcgttc agcaaaaaca gctggcacca aagatttttg
    35941 aaatgacatt gaaaggtgat ctagtaaaag aaatgcagca accaggacag ttcttgcata
    36001 ttctcgtgcc gcgtgcagac ttgctattac gccgaccgat tagcttgaat catatcgacc
    36061 acgaaacaaa tacttgccgt atcatttatc gggtggaagg tgaggggact agagtcttct
    36121 ctactctttc tgccggagac catttagatg tcatgggtcc tttaggcaat ggttttgatt
    36181 tgtccaatct tcatacagga gacgaagtat ttataatcgg tggaggaatc ggtgtccctc
    36241 ctctatatga gttatcaaaa cagttagtcg aaaaaggaat taagccaacg cattttttag
    36301 gttttgccac acatgaagtc gtttattatg aaaaagaatt taatcgtcta ggagaaacaa
    36361 gaatcgctac tgacgatgga acatacggta tgcacggaaa tgtcggtaat ttattgttag
    36421 gaacgaagac ggaacctaca gctgtttttg catgtggttc aaacgggtta ttgaagacag
    36481 tggaacagct gttttcttcg catcaaaatg tccagttatc attagaatcc cgcatggcat
    36541 gtgggatagg ggcttgctat gcttgcgttt gtcatacgcc ggaagatgaa acaggaacaa
    36601 aaagtgtgaa agtttgtgac gaaggaccga tttttaaaat aggagaggta attatatgaa
    36661 tgagaatcct ttagcggtca aacttcctgg cctagaatta aaaaatccta tcatgccggc
    36721 aagcggctgt ttcggttttg gcaaagaata cggaaaatac tatgatttga atcagcttgg
    36781 atcgatcatg gtcaaggcaa cgactccaaa tgcccgcttt ggcaatccca cgccaagagt
    36841 ggcggaaaca ccatctggca tgttgaatgc aattggtttg caaaatcctg gtctagacgt
    36901 tgttatgcat aaacttcttc cagaattaga ggagtacaat gagttaccca tcatcgcaaa
    36961 tgttgctggt gcttgtgaag aagactatgt agaagtttgt agcaaaattg gcgaagcacc
    37021 aaatgtgaaa gcaatcgagc tgaacatttc ttgcccaaat gtcaaacatg gcggaattgc
    37081 ttttggaaca gattctgatg ttgcttttca attaacacaa gcagtaaaga aagtttcttc
    37141 tgttcctgtc tatgtcaaat tgtccccaaa tgtaacagac atcgttccaa tcgctcaggc
    37201 aatcgaagct ggaggggcag atgggtttac gatgatcaat actcttcttg gtatgcggat
    37261 tgaccttaag acaagaaaac cgattttagc caatcaaaca ggtggactct ccggaccagc
    37321 aatcaagcct gtcgctattc gtttgattca ccaagtagca agtatatcca gccttccaat
    37381 cattgggatg ggcggcgtgc agacagtgga tgatgttctt gagatgttta tggctggagc
    37441 aagtgctgta gctataggga ccgcgaattt caccgatccg tatatctgtc ctaaactaat
    37501 taaagagctc cctgtgcgaa tgagcgaact aggcatcgaa tcgctggaaa gattaatcaa
    37561 agaagtgaga gaggaacgtg aacgatgaat caactagacc aacgaccaat tattgcatta
    37621 gatttttcaa ctaggcaaga ggtagaagac tttcttcgat ttttcccgaa agaagaaaaa
    37681 ttgtttgtga aaatcggaat ggaattgttc taccaagaag gacctgaaat cgtacgttat
    37741 ctaaaagagg ctggtcataa tgtattttta gatctgaaac ttcacgatat tccgaatacg
    37801 gtcgaaaaag caatgcgggg attggcaaag cttggtgttg atctcacctg tgttcatgcc
    37861 gctggcggta ttcgtatgat ggaggcggca atgcgaggat tagaagaggg gactccagaa
    37921 ggtggaaaac gtccgctact tctagctatc actcagctta cttctacaag tgaagaagaa
    37981 atgcatgccg atcaactaat cgaagtttct ttagaaaaaa gtgtgatcca ttatgctagc
    38041 tgtgccaaaa aagcaggatt agatggtgtg gtttcttctg cttgggaagt agaagcgatc
    38101 aaagaaatgg caggagacga atttgtttgc ttaactccgg gtataagacc agaaggaaca
    38161 gtcgcaggag atcagacaag agtagtcaca ccttctcagg caaaaaagat cggctcaact
    38221 tttatcgtta taggtcgacc aatcactcag tcaactaatc cttataaagc atatcaaacg
    38281 attcaaacag aatggagcta acctaaaatg aatgttgaac aaagtatcgc aaaagactta
    38341 ttagaaattg aagcagtatt tttaaatcca agtaatcctt ttacatgggc aagtggaatc
    38401 aaaagcccaa tttactgtga taaccgaatc acgatgagct atccaaaagt ccgaaaagag
    38461 atcgcaaaag gattagcaag caaaatcaaa gaagcttacc ctgaagtaca agtgattgct
    38521 ggaactgcaa cagcaggaat ccctcatgcc gcatgggtag cggaaatact ggatttgcca
    38581 atggtctata ttcgaagtaa ggcgaaagat catgggaaag gtaaccaaat cgaaggacgt
    38641 atatttgaag gtcagaagat ggttgtgatc gaggatttga tttctacagg cggaagtgtc
    38701 ttggaagcag ctgaagcagc aaaaagagaa ggtgcggata tccttggtgt tgcggctatc
    38761 tttacgtatg agttgccaaa aggaaaagca aatttcgaaa aagccgaaat ccctcttatg
    38821 acgttgacca attattctgt attgattgaa gcagctttag aagatcgtta tatcgatgag
    38881 caagaattaa ctctgctaaa agaatggaaa aaagatcctg aaaattggca gatttaagcg
    38941 cgcttgatga agatagagaa atccgatttg tgtctccaga atcatcaatt ggaatagcaa
    39001 aaatccaaaa actgattgcg agaagcatct tttcatcagg tatgaaaaga aacttctatc
    39061 gtgatcagtt tttgattttt ttaaaattca tttttctaag actggttagc agaaagaaga
    39121 gaataatctc ttctcttttt tctattatat atagattttc tctatttttt ggtaagggag
    39181 ctgtttcaaa aatggtaaag agcggaaatg ttgcgaatat cagttattta atgatatgat
    39241 attcggaaaa agaaatctca aagggaggta acttttatgg ggttgaatga atttatttta
    39301 ggattgaaca atttagagac gatcacaagg gcacctggat tttttaaata tacagagcat
    39361 acagttgcag cacattcgta tcgtgtagca tctattgcgc aagttctagg agacattgaa
    39421 gaagcgaatg gacaaccgat cgattggaaa ctgctctatg aaaaatcatt gaatcatgac
    39481 tacacagagc gttttattgg tgatatcaaa acgccggtga aatacgcaag caaagagtta
    39541 agagggatgc tgcaaacagt tgaagaaaag atgacggacg aattcatcaa ccaagagatt
    39601 ccagctgagt tccaagaagt ttatcgccgt cggttgttcg aaggaaaaga tgaaaccgta
    39661 gaaggagaac tattagccat ttcagataag gttgaccttc tttatgaatc ttttgatgaa
    39721 atcatcaaaa ataacccgga aaaagtttat aaggagatgt tcattgaggc agtgactacg
    39781 attaaaagct ttgaacatcg gccttctgtt cagtatttct ttaaagagat atttccggaa
    39841 ctattgaacc aggaatttta tggaaaagag gcgtttttag aaaccgtctc aacgattgta
    39901 aagaaagaag aaaaataaaa atgatatgag atgcaggggg aagtatagtt gaaaaagaat
    39961 atggatgtcg aatggggcta ttctggagac aaaggtccgg aaaattgggc gagtttatgt
    40021 gattggtttg cgagaggtgc ggaatttcca ttgcagtcgc ctattcattt gaccaagggg
    40081 gaagaaacca aaatagaagg agacacgctt tcctttcatt atcaaaagca acggtttact
    40141 gacaaggaat tcaaaaacac gattcattta gtaccttttg accaactcag ttatgtagag
    40201 ttcaagaaag aacgctatta tttgacagat attcatttcc acttgccttc tgaacatatc
    40261 atcaacggaa aacaggaaga tattgaattt cattttgttc atatgaacag caaaaaagag
    40321 aatctagttg tgggtgtcat gtaccagcta atggtacagg aaggttggct gtataatcaa
    40381 gaaaatgggg aaagttggaa cttagaaaaa catgagcatt gggtaaatcc ggatgtgttc
    40441 tttccggagc aaaaaagcca tttccattat attggctcat tgacaactcc gcctaccagc
    40501 gggccaatcc aatggtttgt aatggatcat gtaggaaaac tgaaccaaga gtttcttctc
    40561 cccttcgatg gtcagtatgc tcgaccgaac aaccgaccaa tccagccctt gaatggaaga
    40621 gaaatttatt atttcgagga gtttgagcaa tgaatattca gcagatgaaa tacgtggtcg
    40681 ctgtagcaaa caatggtagt ttcagagaag cggcaaagaa aatgtttgtc tcccagccaa
    40741 gtctttcagc tggtatcaaa gaattagaaa ccgagttagg gatcacgctg tttactcgaa
    40801 cgaatcgagg ggcatattta acagaagaag ggcaagactt cttaaatcgt gccgagaaaa
    40861 tattggtcca gcttgataca ttagaaactc actatctaac gagtgaaaag agagacaatt
    40921 tttcgatcgc tgcccagcat tatgatttct taggtccttt gaccgcacag ctactcgagg
    40981 aattcaaaga cgagatcaaa caatttcgga tcatggagac gacaacagcg agagtgatcg
    41041 aagaagtcaa agagcagcac agtgagatcg gcattattta tatgaatgag cataatcaag
    41101 ctggtatcag aagatattta caacaaggag agctaagtgg ccgcagcatt ggaaaattca
    41161 aaacgcatat ttttttaagg caaggacatc cattagctga acacgcagag atcaccaaag
    41221 aagaattgtt atcttatcct caagttcgtt ttacacagga tggaaataat ttcccgtatt
    41281 tttatgaaga ccttatcgag atccctgatc aagaaacagt gatttatacg agtgatcgag
    41341 gtactttgat gaatatcgtt cttgaaactg atgcttacgc ttcaggatct ggtattgtga
    41401 ttggagggat tcgcgagcac ttgcggttaa tccctttggc agaaggcgaa ctaaatgaat
    41461 tttatatcat ttattcggcg aaacgtccgc tcagtgaaat cgctcaaaga tttatcaaag
    41521 agcttactcg gttactggac cagcagagct aagaaaagca gatgagaacg ctgtttattg
    41581 aacaccgttg atcaaaaaag aggggttgtg acagaatttt gtcacaactc ctctttttgt
    41641 agcaaacaat ttataactaa ttgctgttgt gggaaagtac gcaaatatga ttctagaaac
    41701 actcattgat tacctgtttt gtttcatagc tgtaattttt tcttcttctg gtgtcacgat
    41761 aatcgtcttt tggttttcat aaataacata accaggcttg gcgccatttg gttttcggat
    41821 atgctttacc tgtacaagat caaccggcac ttgggcagag tatcggtatt ttgaaaaata
    41881 ggcagctaac tcggcagctt ctgtaattgt ttcatctgag ggcttgtcac ttttaatgat
    41941 gacgtgcgaa ccgggtatat tttttgcatg aagccaatag tcggtttttt tagctgtctt
    42001 cattgtcagc tggtcatttt gaagattgtt tttaccaaca agaatgagcg ttccgtcaga
    42061 agaaaagtat tgatcaggct gagatggttt cttacgtttt tgttttttta agttcttctt
    42121 tttcaggtat ccttctgaag tcaattcttc tttaattgct tcgatatcca ttgggccggc
    42181 aatttccagt tgagataaga cagattcaag atattggatt tcatctttgg cttcttggat
    42241 ttgttcgcca atcagcttaa cagcgttctt cagcttttgg tatcgatgga aatatttctg
    42301 tgcattttga tttggtgtca atgccggatc tagtgctatt ttgattggcc gatcttcttc
    42361 ataataatta ggtaaaacaa cttcttttgc gccgcgtggt acttgtgtca gaaaagtagt
    42421 aagtagttcg ccatttctac ggtaattctc agcgttttcg gattctttga gtgtttgttc
    42481 acgttttttc aatttatttt tatttcgttt tagttcattt tcgatttttc gaatcagttc
    42541 tccgccttgt tgttttgccc gatctttctc tgctttttcg tgataatagc tatctaataa
    42601 ttgactgagt gttggataag ctgttacagc agatgcctgt tcggacaata cctgataagc
    42661 aatcggtgtg aaaaaatctt tattttccgt ttcgtaaaaa gtaggaatag gctgatggct
    42721 gatggctgag aaaaattcgt cccatacaac catcttttca ttaggacgtt ccagtaatct
    42781 tttggtcagc tcttgagcga tatcgaaacc gattccctga aactgacgtt ggattgcttt
    42841 tggatctagt tcagtttggg aaagccgctg aaagattttt tctttctcgg agctaaatgg
    42901 gttcaagacc tcttgttttg gcggagcaac atattctaca ccaggaagca aagaacgata
    42961 agtgttttga gagcttccaa tgtgtttgat cgcatccagt atttttccag tttctcgatt
    43021 aactaagacg atcgtactat gacgtcccat taattcaacg atcaatatga tattttgtag
    43081 atcacccaac tcgtttcgtt tagcaaagtg gaaatggatc actcgatcat tttcgatttg
    43141 ttcgatgtct tctaaaatag cgccatccaa atattttcga agcatcatca cgaagttagg
    43201 cggattatca ggattctgat aatcgatttg ggtaatctgt acacgagcat aacttgggtg
    43261 cgcagataat aataatcgat ggtttttgcc gcgtgaacga atcaccagca cgatttcatt
    43321 ttcataaggc tgatggattt tcgagatccg gccagatagt aacgtttcac gcaattcgtt
    43381 gaccatcgcg tgggtaaata caccatcaaa ggacatggtt cattcctcat ttcttttcat
    43441 aagtatattc attataacga aaaaaagaag aaaaaccaat ttggccgaat agtaaagcaa
    43501 aagtctagaa aaatcttgga ctaattacat aaaaaacagt gtttctatcg ttgaaatcca
    43561 aattttta
//
LOCUS       DACDCJ010000014        43209 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDCJ010000014 DACDCJ010000000
VERSION     DACDCJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.014"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..676
                     /locus_tag="IV038_000774"
     CDS             <1..676
                     /locus_tag="IV038_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8341015.1"
                     /translation="RAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGK
                     QTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQ
                     AIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGL
                     NENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSID
                     DLSNLI"
     gene            complement(725..2014)
                     /locus_tag="IV038_000775"
     CDS             complement(725..2014)
                     /locus_tag="IV038_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8341016.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(2041..2343)
                     /locus_tag="IV038_000776"
     CDS             complement(2041..2343)
                     /locus_tag="IV038_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8341017.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(2368..4461)
                     /locus_tag="IV038_000777"
     CDS             complement(2368..4461)
                     /locus_tag="IV038_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8341018.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(4950..5714)
                     /locus_tag="IV038_000778"
     CDS             complement(4950..5714)
                     /locus_tag="IV038_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP8341019.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(5825..6694)
                     /gene="mdcB"
                     /locus_tag="IV038_000779"
     CDS             complement(5825..6694)
                     /gene="mdcB"
                     /locus_tag="IV038_000779"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP8341020.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(6719..7795)
                     /locus_tag="IV038_000780"
     CDS             complement(6719..7795)
                     /locus_tag="IV038_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP8341021.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(7819..8412)
                     /locus_tag="IV038_000781"
     CDS             complement(7819..8412)
                     /locus_tag="IV038_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP8341022.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(8416..10092)
                     /gene="mdcD"
                     /locus_tag="IV038_000782"
     CDS             complement(8416..10092)
                     /gene="mdcD"
                     /locus_tag="IV038_000782"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP8341023.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(10082..10381)
                     /locus_tag="IV038_000783"
     CDS             complement(10082..10381)
                     /locus_tag="IV038_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP8341024.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(10483..12129)
                     /gene="mdcA"
                     /locus_tag="IV038_000784"
     CDS             complement(10483..12129)
                     /gene="mdcA"
                     /locus_tag="IV038_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP8341025.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(12136..13047)
                     /locus_tag="IV038_000785"
     CDS             complement(12136..13047)
                     /locus_tag="IV038_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8341026.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            13167..14024
                     /locus_tag="IV038_000786"
     CDS             13167..14024
                     /locus_tag="IV038_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8341027.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(14211..14573)
                     /locus_tag="IV038_000787"
     CDS             complement(14211..14573)
                     /locus_tag="IV038_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341028.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            14919..16358
                     /locus_tag="IV038_000788"
     CDS             14919..16358
                     /locus_tag="IV038_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8341029.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(16365..18917)
                     /locus_tag="IV038_000789"
     CDS             complement(16365..18917)
                     /locus_tag="IV038_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP8341030.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(19031..20362)
                     /locus_tag="IV038_000790"
     CDS             complement(19031..20362)
                     /locus_tag="IV038_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8341031.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(20328..21806)
                     /locus_tag="IV038_000791"
     CDS             complement(20328..21806)
                     /locus_tag="IV038_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8341032.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(22111..23946)
                     /gene="tdc"
                     /locus_tag="IV038_000792"
     CDS             complement(22111..23946)
                     /gene="tdc"
                     /locus_tag="IV038_000792"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8341033.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            24416..25843
                     /locus_tag="IV038_000793"
     CDS             24416..25843
                     /locus_tag="IV038_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP8341034.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(25935..26300)
                     /locus_tag="IV038_000794"
     CDS             complement(25935..26300)
                     /locus_tag="IV038_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8341035.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(26297..26662)
                     /locus_tag="IV038_000795"
     CDS             complement(26297..26662)
                     /locus_tag="IV038_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP8341036.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(26854..27102)
                     /locus_tag="IV038_000796"
     CDS             complement(26854..27102)
                     /locus_tag="IV038_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341037.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            27101..27229
                     /locus_tag="IV038_000797"
     CDS             27101..27229
                     /locus_tag="IV038_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP8341038.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            27286..28980
                     /locus_tag="IV038_000798"
     CDS             27286..28980
                     /locus_tag="IV038_000798"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP8341039.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            28984..29103
                     /locus_tag="IV038_000799"
                     /pseudo
     CDS             28984..29103
                     /locus_tag="IV038_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            29749..30882
                     /locus_tag="IV038_000800"
     CDS             29749..30882
                     /locus_tag="IV038_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP8341040.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            30913..31233
                     /locus_tag="IV038_000801"
     CDS             30913..31233
                     /locus_tag="IV038_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341041.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(31262..31726)
                     /locus_tag="IV038_000802"
     CDS             complement(31262..31726)
                     /locus_tag="IV038_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8341042.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(31857..33110)
                     /locus_tag="IV038_000803"
     CDS             complement(31857..33110)
                     /locus_tag="IV038_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8341043.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(33139..33456)
                     /locus_tag="IV038_000804"
     CDS             complement(33139..33456)
                     /locus_tag="IV038_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8341044.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(33482..33802)
                     /locus_tag="IV038_000805"
     CDS             complement(33482..33802)
                     /locus_tag="IV038_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8341045.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(34266..35681)
                     /locus_tag="IV038_000806"
     CDS             complement(34266..35681)
                     /locus_tag="IV038_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8341046.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(35698..37014)
                     /locus_tag="IV038_000807"
     CDS             complement(35698..37014)
                     /locus_tag="IV038_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8341047.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(37164..37862)
                     /locus_tag="IV038_000808"
     CDS             complement(37164..37862)
                     /locus_tag="IV038_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP8341048.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(38151..39139)
                     /locus_tag="IV038_000809"
                     /pseudo
     CDS             complement(38151..39139)
                     /locus_tag="IV038_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(39219..40052)
                     /gene="msrA"
                     /locus_tag="IV038_000810"
     CDS             complement(39219..40052)
                     /gene="msrA"
                     /locus_tag="IV038_000810"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8341049.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(40227..41855)
                     /locus_tag="IV038_000811"
     CDS             complement(40227..41855)
                     /locus_tag="IV038_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341050.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(41842..43176)
                     /locus_tag="IV038_000812"
     CDS             complement(41842..43176)
                     /locus_tag="IV038_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341051.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        13170 a         8885 c         7332 g        13822 t
ORIGIN      
        1 tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt tcaaaaaggg
       61 gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg gacatcaaga
      121 aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg attatagcca
      181 attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga aacataaaag
      241 tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga aaccgtgtgg
      301 tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg taccgaaaaa
      361 cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga aacagatcag
      421 taatgtcaat gatattgcca tttatttcgc tgatccagga acgccgtctc aaagaggcct
      481 aaacgagaat tctaacggat tgttacgtag agatggttta ttgaaatcta tggatttcaa
      541 ttcagtagat gaatttttta ttcaatctgt cgcatctaaa cgaaataata ttcctagaaa
      601 atcactggat tatcgaacac ctttggaagt atttttgagt tacgtaagta ttgatgatct
      661 gtctaactta atttgacaat taagatttct taaaaaatcc aaacatcatt ttccctactt
      721 tcaattaaaa tgctttcaaa actgggctta agatccaggc atataagagt gctgcggcta
      781 ctgtatctac atcggttgct gtagcattga tgaatcccat cgaattaaag atcgttacta
      841 gcaaagcggg caataatgta ataaagaaac catgagcaat accgccaatg atcgctcctc
      901 ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag aaattggtta
      961 gcataccagg taaaatcatc gctaagccga acatgggcaa agtaaacatg gcaataatcg
     1021 taccaatcgt tgtcgtaatg aaacctaaaa tgacagcatt tgggctataa ggaaagaaaa
     1081 ctggacaatc taaggcaggg atcgcatcag gaactaattt catagctatc cctcgaaatg
     1141 ccggcacgat ttcacctaac agtagacgta caccagctag caatacatac acacccacta
     1201 cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct gataattcag
     1261 aagcgaatgg ttcgcctgca aataaaacag tgatgatata tagcgggacc atcacgacca
     1321 tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga
     1381 tcgacttttt attttcgcct ttctcaccaa aaagcttagc aacaccggct tcaaataagt
     1441 acccgatcgt acaaaaatga cctaaagcaa tatcatttga tccagtgatt ttacgaatga
     1501 tcggttgcgc tactgccggc atagcaatgg cgaaacaagc tccgataaaa tcaccgacca
     1561 agatcaatac gatccctcgc agaccggcaa aatagccaaa gactgtcgtc atcgttgcca
     1621 tccataaaat tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa
     1681 taatattgaa tacaaaaatg gctaagaacg tcagtgcaat atctctacct aaacccaaat
     1741 cattcattgc ttgaccattg atagattcaa tagaagggat gatcccttgc atatgaaatc
     1801 cttcagtaaa gattttcccg aaataagtta aacttccaac gatgatgctc gaaccggcac
     1861 tcaacacttg aaatcctagg agtgtcttta acgtgccaga gatgacttgt cctgctgatt
     1921 tcttctgcag tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca
     1981 aaatattttc gatgataaaa ttgataacgc ccatgttttt accctctttt ctttttgtat
     2041 ttagataact cccttatctt gcagaactgg ggtgattttt tcttcaattt cattttttga
     2101 aacaatattt ttcaaataga tcatcgccgt tttaggatca actgcgaact tttcgaactg
     2161 cgaacgaaaa ttttctgctg tgatgatcac atctggtttc atggaaactg cggaagaaat
     2221 atcacaatgg tccagtttgg cttctactcc taatttgttc aaaacatcct cagcagacat
     2281 ctgtgctgca aaactacttc ctaaacctgc accgcatacg aataaaatgt tcaatttgtt
     2341 catctttgtt ctcccccgta tcatttatta tttctcaact actgaaaaaa gcagttgttt
     2401 gaaatcttcg atatttgtgc attcaatcaa tcgatccaac ttctcttcgt tgttgatcaa
     2461 ttcaattagt tctttcatga tgttcagatg cgagtaggag tctactgctg ccaaacaaaa
     2521 aataatcttt actggatcca tttcaggatt accgaaatcg attggttgct caatcgtagc
     2581 tacgctgact cctaatttat tgaccccatc ttctggtcgg gcatgggcta gtgccatgtg
     2641 tttgccaatc acgatatacg gtccgtaatc attcactgca cgaatcatcg cttcgacata
     2701 gcgctcttcg acaatgtttt ctttaaccaa tggtctagca gctagttcaa tcgattcctc
     2761 ccaagtagtg gctttttctt tgatcatgat attgctgtcc gtcaaaatat cttttaacat
     2821 aggctggatc tcccttttgt tgatttctaa attgtttgtt tcgaataatt tttctaactt
     2881 gccatagatg attcgatcta ctcggccacc actttctttc atgacatcta ttatttgagt
     2941 gaataactcc gtcgattcat gactgttagg ctctaaccgc tgtagttctt catttctttt
     3001 caaaaagtct tctataacca agatattcgt ctcagttaaa atcggatcga caactaaaag
     3061 cggcttattt cgataattca attgaaaggt gctgaaaacc agattgacat ccaatttatc
     3121 tactaattcg atttcttttg aacttaaaac tgcagtaatc tcgatctgag gaaacttttc
     3181 tttcagattc tctgctaaca gatttccagt agccatccca tggttacaga caacgactga
     3241 tttataaaca taattcaagt ctcgcttgat tacactggcg atcgtcgaaa agtggatgac
     3301 aagaaaagct atctcgtcct ccagtatctt gccaccaatt acttcttcga tcgtaggaac
     3361 aaaatgagtg actgcgcggt agattgggaa ataatttgtt cggatgtttt ctttcaatgg
     3421 attcatgact tggatatgat tcttgattct tacaatcagt gcagccatgt gcttatagag
     3481 attttcgaat aaagcttctt cctttagatg gaaaggtacg tgtgtctcgt tctctacaaa
     3541 ttggatcaat tggatcgtta gtaattgagc ttgtacccac tcgattgaat tggagatatc
     3601 tctagcattg aacgtctcaa tagtgaaacg aagataattt ctttctactt gcgggacatt
     3661 gtccaattga aattcctcga tcatgtggtc aataaatgca gaaaagctac tgtcttttat
     3721 ttctgtattt ttccaattaa tggcagagat aaattcatgt ttcctgattc gctggatcca
     3781 gatcaatgta aaaagcagga tttgtttttt ataaatatca tcttcttgtt tcaacaactt
     3841 attggaatag atctcctgta tttttttgat ttccaccaaa ggaaaataat ggagaaagat
     3901 tttttgagcc ggtgttaatt gtgcatcttg attcataaag aagtcggtca ttcccagatt
     3961 ctgattgata aaatcataga tcatcgtacg gatagatcgt tctgaacctt tataaaccag
     4021 tccttgcttt ccataactga cgatctctat cccataactg accagtgttg ctcgtaaccg
     4081 tctcatatct tcatctaatg tacttttaga gatttgaaat tcttcttctt ttttgtttag
     4141 atggatcggg attcggctgt aagcaacggc taaaactaga tcaaaaatac gttcgcgccg
     4201 attcaaaaag ccgtctttca catcttttat ttcagtttct aatcgagctt gcttttcctt
     4261 ttccagcttc aacaaaaaac ctttgttgcg aattgtacgg atctccggaa gctttttact
     4321 gctcaaccag atattcgctt caagaacttc ctgacggatc gtccgctgac tgacattgaa
     4381 ctcacttgct agatgttttg cttttactgg atattgggct gtcatgattc tgtgaagtat
     4441 ttcaattgtt cttgttttca tagaaatgat ctccttctga tttcaagtat atggttccgc
     4501 ttacataata aaaagtagat atttctgcca cttgaagtgg aaagaaagta agcaaagcaa
     4561 acaaaaaata agccatatgc tttttcatct cctcaacgaa aaagacatac gcctattatt
     4621 acaaaaattt tgcccatcta tacaaaaaag aggttgtgaa aaagctgtcc tgcatcaagt
     4681 aataagaaca actaaactaa aatagtgctt cctatttttc gtttggttgt tcttattatc
     4741 gcagatgcaa gcttttaaac accatttagt ggagaggttg tgacagagat gttcagcctt
     4801 gagcgataag aagcgatttt gaaaaatcgc ttcccatatt ttacaaaatc gtgccttatt
     4861 gccgataggc tatctctggc acaccgttta ttcagaaaaa ggagctgcga caaggctttt
     4921 gtcacaactc cgttcataaa tatttcctgt caatccagca taatcggctg aataaaatcg
     4981 gcttgccatc ttttttcttt ctcagaaaaa tcaaaatacc atatctgaga aaaatactgt
     5041 cgttgtgtct tacttcctga gatcaatcgc ccatcttttc gatcgactga ataatcacag
     5101 catctgaaat caattcggat ggcaaaacta ttttcgtgga tctgtcgata atttcctact
     5161 tcctgaatag ttattttttt agtatgattt cttacaccgt ctgctttgtt tttttgcaga
     5221 actttctgat gttcggcaaa cagcttgtct gtataacagc ttctggcttt ttcaggagtt
     5281 tcttcttccc aagcggattg gatcttcatg aatatctttt ggatttccag aaactgtgct
     5341 ttcttttctt tagccgttcc tgacatgcgg gacaatatct ccgctttcag ccttttcttt
     5401 tgtcttttat tcttgacgat agaaaaagca gaaacccctc ctactgacaa aacagctaat
     5461 gcaagaaatc ccgtttgctc ataactagaa ggacctgtac ctggaccata atacccataa
     5521 gaatctctgc cgtaagaacg actgccaaaa taactgcccc catatgaacg accaccaaat
     5581 gaacctctgc ccgaacttcg accgctagat gtacctccac catgaccacc cgcgacagcg
     5641 aatccttggg tcggcagagc aaacaaacat aggcccacta acaaaatgat tgctaaataa
     5701 ttgatttttt tcattgtctt cctcgttatc ttttattaca aaaaactatt tttatgatag
     5761 ccttgctaaa aggaagagtc aataaccaga tgcataaact gtttttttag caattttgtc
     5821 ttatttagtc attgagtaac atcggacaaa tgtatgtgtc ggcagtccat aattcctcta
     5881 tcttcagcat gaacaagctt gcagacaaga gatctgcgct tcctccagga gagatttggt
     5941 tttcctgaca aaagtgcaac agtgctgtaa atggacgatt tggtaaagca ctttgactag
     6001 cagcatgagc aagtttttgc atttccacta acgttttctg atcactgcga tgcaatacgc
     6061 atgtgtcatc tagcgaactc atcaaaacta acaacatatg catttgtttc tgtacatatg
     6121 aatcattatc tagctcctta gctgcttgaa ttgcaagcct gatatgcgga taccctaata
     6181 tcgcttcttc atatgcacca tttacgccat atttttgctt caccctatga ccatgagtgg
     6241 tttttttatg acgatatttt gtatcaggaa aagcagcaag cttgctgaca tcatcaagta
     6301 ttttcgataa agaaaaaggc tgttccatac tgattttttg tgcaatgaca ctgatcaata
     6361 gccccattgc ccaaatcgct cctttatggg tattgatgcc atttgtagca atcagcattg
     6421 ccttttccgc ttttcgccca atctcagcaa ttctttctct taactcctga tcaatagcat
     6481 gatgccatgc tatttgagcc atttcttcaa aaaaaggagt tagcgtctta gcagacttaa
     6541 gaaataaagc atagtccata tcggaatgtg cacccgcatc cagtgcatcc actaatccag
     6601 gttttggact taatgttact tctgcgatca atgcttcctc taccaacgca gcgagttgct
     6661 gagtcactgc tttttttcgt tcgtcattta tcatgacact cactccttct tcctactatt
     6721 acttgctgaa accttttttg atagctaccc aaatgatatg aagtaaccag cttaaagcaa
     6781 atgcacaact tgccgtcatt agataagcta caagaatcat gactcctgaa ggtaactgca
     6841 gccaactatc tttaaacaat aataacagta cttggaaaac aaatacattg gctaaatacg
     6901 tacgataagc ataaacagac aaccatttga acaatggcaa ttttggcgaa tcgtttacaa
     6961 tcattttgga agcgcctatg aagacagcaa agatgatgac taaactgtat aaactttgga
     7021 tcgtattcaa gtaaggtgca ttcgcaaagc tcaagtctcc tggatgtgcg agcaatgaac
     7081 ggacagaaaa gaacgcgata actaaggcaa caggtaagaa aagataacgt actctgtcca
     7141 aatattgata gactttttcg tgataaacaa atgcggctac cccataaatc atgaaactag
     7201 caacaaaccg atctaataag taccagctgt catgataagg cccagtcaat acttgtgttt
     7261 gatagaacag ataccatgcg acatataaaa tcgttgaacc aatcaatact ggccatacat
     7321 attttttatt agcaaatact ttgtaaccga gccaaacaaa aaacggcatg aacaattgga
     7381 tctgcagcat cataacggta taccataaat gaggcgctcc atcacctgtg acatatttca
     7441 gtaaaaagct tgcagtatcc gtatacggag tttgttgttg gactgctgga aatagccaaa
     7501 gataagccgt cgtccataga agatacggaa cgactagttc aaaaaacttt tctttcaaaa
     7561 attcccaata agacaaatgc tgacttgttt ttaccatatt ataaacaatt gcaaaaataa
     7621 acattggtgc tgtgtattta acgatgacgt aaaaggtacc aagaaaagct gtatttgttg
     7681 cgctgtggct gtttgccaaa gcaaagctca atatcgtttg catcataaca gccgttacag
     7741 ctgatacttt ctggaagtca cttacgttga ttttgatttt tttgttttca aacatctctt
     7801 tctctctctt tttctttatt accaaagttc tttggttaat ttaggtcctt cgactgtttt
     7861 caatagaatt tttttctctg gctctctcaa atactctttt agtgaaaaac cgccttgccg
     7921 agtcactact tgggtatcga attgctcaaa aaaagcagga tgcgattgaa ttacctgaat
     7981 tggcaattca atcggtgaat ttgcgtacag cagcaaatca aaatcactgg tttcttttac
     8041 tgccggaatc ccagtcgata attcaaatcc taagctgccg ccgattcccc atttacatcc
     8101 tgataatatg tttctagcag catgaaattg ctgataaaca ggataatcca tgattttctc
     8161 ttgtctatac gattcttgtt cgatgatgtc ccacggatga atgacttgtt tgacggcatg
     8221 ttttgagatt tctgttgacc atctctgatt gcgggcaggt ccacgtaaac caatcggtag
     8281 tatttctgcg tgatccgtcg acgcacgacg aactgttccg aacatctttt cagttagaac
     8341 gacccattca ggcaaggtga acccttctat ttcatgttta tcaaaaagga caatatcatg
     8401 tgcttgaatc attttttatg catcccattc ttcgtccatt ttggcacgga ctttatttgt
     8461 ttcgacacgt ccgaatgttt tggctgtttc gtttgtgtat cgatttgtca gcatcgtgtc
     8521 atttgtattg cggacatctt gaatggcttc attgattgct tcgatgactt tgctgacatt
     8581 ttgttctgaa gcagcttggt cggtgatatc ttctaggaaa tgatagatcg ctcctagttt
     8641 tgtataatta ttgatatcgt aagcgatagc tggaactttt tcagcagctg cttcgatttc
     8701 agcaaccgaa cgttttgtca cacgtgcagc actttcttta gacatcgctt ggacggtaat
     8761 ttctttattg tttaaagcaa tcaagcggct tgattgtaat ccatgtgcta aaaatgcacc
     8821 tgaaatagca tttcctgcaa tcaatccaat aactggatgt ccagcttgac ggagttttgc
     8881 ataagcagca gcactattag ccaaagccac atggataccg atcaattctt cgttatagcc
     8941 gtatgcttgg cttggtacat cgatcacaac gatgattggc cgtttcactt ttttgtcttt
     9001 atcttcttcg taaacgtgat ccaaaatttg tcccatgcgg aaaccttcaa ctaaaccaac
     9061 ttcaccgttt cgtacacggt acatcgggtt tttaggatca ggtacgattg atgtcacgat
     9121 ggcttcttct atgaatttac ttgatttacc ataatacaca gtactaatag aagaagttgg
     9181 atttttcatg ccagttagtg ccgcaaacca atcgtaacca acacttgctg cagctggttc
     9241 atttccttct gctacttctg gtaagtcaat tgattttgct tgatggttag caattgcttc
     9301 attgtaatcg tcaatggcca ttggttttga aagatctaaa gaatcaagta atgataaata
     9361 aaatgctgag ctttcactgc gatgcgtgtc ttttcgttcg tccattgctg cgatgacagc
     9421 agctttgatt gcttctacac tatcagtcac taattcatca atgatttttg attctacccg
     9481 ttgacgagat cctaatgtat tccaaatcaa gtctttgtca ctagaatcaa attctcgaac
     9541 gcctgcttct tgttcaataa cttctggtcc attcaaaccg actctcgctt tttttgttgc
     9601 gattaaataa ctcattaatg ctgaagtaat cgacataccg ccaaatgatc ctacacgtcc
     9661 tggaatcaaa ccgatgactg gtacatattg ttttagagca atgattacat ttccgatttc
     9721 tgaaatagat aacagtccgt agtttgcttc ttgcaaacga acaccacctg tatctagtac
     9781 aatgactgga aagatctcat ttcctttttt attttcttct aacacatgtt caagtgcagc
     9841 aacgattttt gcaccggaaa cttcaccgat accgccacct tggaattttc cttccatagc
     9901 gattactact gcttgtttgc cattgatcgt gccacggctg acaacgatcc cgtcatcact
     9961 ttcagggacg attccttgtg ctggtaaact tggcgcgatg atttgatcga atggatcaat
    10021 caattcacgt ccattgtcaa gtagagcgtt cactcgttct ctggcgttta attctacaaa
    10081 actatttttc atctgaaagt tcctccattg cttgcgtcaa tcgtaggtac acaactcctg
    10141 gtgtagcacc gaagtcattg atcacgatat ctgctgaaat tggataacga tcaaaaaatc
    10201 tcgttaatac attgttccat acttcgccga agccatcgct tcctgtacaa atttttacag
    10261 tagattctgt tttttctgtt ggaaacatca agatttccaa atctcctgaa ccgaccactc
    10321 ctacatggat tttcttattg atcggctgtt tcgttggata tttataagct aattgttcca
    10381 tggatctctc ccctttttgt aaaacagagc atacacgttc tgcttattcg tctttcttct
    10441 tccatctggg taaatggcga caaaaggttt ttctcgccaa ttttatgacc aagttctgaa
    10501 tttcattggt ggttcataca ggccatctga ccattcgatc aaatcatcga tggtttgtgc
    10561 agctaataat gaacgtttcg cttcactacg tttgatgcct agatcttctg gtaatttcac
    10621 gatgccggct gcacgcaagc tatctagtga ttctttcgtg ctagtcattc caagaggtgt
    10681 aacaccgcca attgctgcaa ttgctgcttg tcgttcttct tttgtttttg ctttgtacaa
    10741 ataagcaatt ccttcttctg tcacgatatg tgtagtatct tcgctataaa tcatcactgg
    10801 tgcattggct aagcctgctt gtttttgaac agctagtgca tctaattctt cgacaaagac
    10861 tggtttttta tttgaaccat atgtttcaac catttgaaca actaattttt ggcctttccc
    10921 taatggatca tcattgtcgc gaagtgattg ccatgctggt gtagaatgac gacgaccgcc
    10981 aggattatgt cccatgttag gcgcaccgcc aaaaccagat aaacgtcctt ttgttacggt
    11041 agaagagttt ccttcgtaat ctaattgcaa ggttgaaccg ataaacatat cgatggcata
    11101 ttgtcctgca gcttgtgata atgttcggtt actgcgcatc gtgccgtctt taccgacaaa
    11161 aaagacatct gggcgagcag caatatattt ttccatacca acttcgccac caaaactatg
    11221 gatgctttca acccaacctg attcgatggc tggaatcatc gttggatgtg ggttcaatac
    11281 ccagttcttc gcgattttcc cttttaaccc tagtgattca ccataagttg gcagtaacaa
    11341 ttcgatagct gctgtattga atccaatacc gtgattcaaa gattgtacgc cgtgttctgc
    11401 gtagattcct ttgattgcca tcattgccat tagaatttgc aattctgtga tgttttgagg
    11461 atctcgtgtg aataaagctt ctaattgata aggtttatct gctttcacga caacgtctac
    11521 ccagtcacca gggatatcta cacgtggtaa agaatcgaca acttcgttga cttgaacgat
    11581 cacgatacca tctttgaaag ctgcagcttc gatgattgtt ggtgtttctt ccgtattgaa
    11641 acctgtgtac aagttgccgt ctttatctgc tttatctgca gctactaaaa caacatttgg
    11701 aatcaaatcg atgaataagc gtccataaag ttccaaataa gtatggatat cacctaattt
    11761 cattgtgccg tcttcaatca tttgggcgac acgcaagctt tgtgcaccag cataagagaa
    11821 atcgatcttg cttgcgattc ctttttcaaa aatattcaaa tgttctggtc gtgagatact
    11881 agacatgatc aaatgcagat cattcacttt ttctgcatct actttttcaa gactttctga
    11941 aagaaaactt gcttgttttt gattgtcgcc ttctaataca actttgtcgc cgggagcaat
    12001 cagtttttcc attacttcta cgattttttc agtttctaca tacttgccat tgagcaagtt
    12061 gctgattttt tccaatcgct gcgctttttt ttcgcgttta gaagcccagt tgcggtttgg
    12121 tttttccata attagtcatc tccatttttg atatagaaac gtggcgtcat caataccatg
    12181 ttggtctaga ttgatcgtac ggatcccttt atctacacca aattttgtat gaatcagctt
    12241 tgtcaaagta ttgccggggg gaaattcaat cgtcagatcc attcctaatt cttttgccaa
    12301 gtccatcatt tggttccact gcacagggaa agctaggttt tccaacaaat ctttacgaat
    12361 ctcttctgcg tcctgagtgg ctcttccatc cgtatttttt atatacaaac atctagcatc
    12421 agatagatgg acagactgca tatacggctt gaatttcttg accgtatcat tcatcaagag
    12481 acagtgagat ggcacaggga ctttcagcag cttcgctgtt tgcgcttggt gccgtttggc
    12541 ttctgccaac gtaacggata aaccttcgat actcccagat aacgtatgtt gcatcggaca
    12601 attttcattt gacagataaa ctggatggtc aggatcaaat gtttttgcta caatttcctc
    12661 agcttcgctt ctcgtcacac ctactactac gcccattgca taaccgcttg gataagcttc
    12721 tttcattaag actgcgcggt gatagactag ttccgctgca ttctcaaatc tcaaagatcg
    12781 acaagcaaca gctgcacaaa aagcaccaat cgaatgtcct gctgcaatat caggctgaat
    12841 accgtttttt tccatttcat ctaaataaaa tactgctttt gtcagtaatg ctagctggat
    12901 aaaaaccgta tcttgatagt tttcttcttt atcaactaac cgataccccg tcacattttc
    12961 taaacgttcc atgtacattt cagggatatc cgctaacatc cctaacttct gactgccttg
    13021 tccaggaaat aagaaacctg tactcatgag cctcgcctcc tcagcactta ctttttccac
    13081 tgctatctta gtcaatttct gctataataa aaaatgccaa aaaaagaaaa aaccataaca
    13141 aaaaggtatg gatgggatgg ataattatga aattgcttca tctgcagtat tttaaaaaag
    13201 tattcgaaac aaaaaatgtt acccaagcag ccaaagaatt atttatttct caacctgcct
    13261 tgagccgtgc gatcaaacat ttggaaaacg aattaggtgt ccctttgttt taccatagcg
    13321 gcagaaacat tgagacgacc gtttatgcag aagaatttta tccatatgca gtcaagacat
    13381 tagacacatt agaagaagga atcaaattat tgtcttcggt caatgagaag atcgttactt
    13441 ccgtcatatt gtatttagag gtagcctctg tttctattcc gaacttggtc cgaattttta
    13501 tggaaaagca tcctgatatc caattaacga tcatgcaaca tcatttacca gatgatatta
    13561 ccgatcctgt actgtacatc acttcggaga tgaagcctgg catgaccaat attcctatca
    13621 tgaaagaacc tgtttatgtc gccattccta agaaacatcc tcttgctaaa aaagaacatc
    13681 tgcaattaga agatatccaa cgcaccccca tcttgatgct gtcaaagagc aacgccttcc
    13741 gtcatacact ggatgcagca cttgaagcga aaagtatcga attgacgatc agttcgacga
    13801 cagatgatcc tgcgacatta cgttctattg tcaagcaagg attagctgtt agctttttcc
    13861 caaaagtttc ttggtcttac gataaaaatg atcctttcgt cttgcgggaa atcaaagatt
    13921 tgcccttgac acgaacaatt tatttatctt ctagcttcac agaagatcat cctttgatca
    13981 aaatgattgc aaaaacgatg cgggaatttt atttaggaaa ataaaaaaaa aagagctggg
    14041 acaaaattct tgtcgcagct cttttttcga acaaacggtg ttccagaagt aatttcttag
    14101 aaaataagcc gaaatttccc aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    14161 cttgaagcta aacatttctg tcacaacctc ttctctattg tcctagacat tcattgcgtt
    14221 gatttaaaac ttccatcaat caaatctgct agttcatcta gcttttctat tttacgtaga
    14281 tcaatagaga tccaatgttc tttattcatg tgataggctg gaaaataccc ctcttttcct
    14341 cgcaaaattc cgccaagttc aggatcgacc ttcaaatcaa gaatattgat gatcctatgg
    14401 tcacttgcat catcatagat tttttcttct ggaacagaca tcaggacccc aaaccatttt
    14461 ttgctttttt gatgtctgaa tacgcaataa tcaggaaatt ttttaaaaat atattctgga
    14521 gacaaattgt atgtttcatt gatgtgatcg attagttctt ttttcgttag catctgcttc
    14581 catctctttc ttattgattc gctttattca aagataccac gtaagaatag aaaaatgaat
    14641 acaagtagtt gctaatcaaa tacttcgata tttttctttt ttatcaactc ttaaatgttc
    14701 aatccgcctg tgttggtctt gctaccatct actattaaaa tgtttattat tgtttgccca
    14761 cctatttttt gattttagtg gtatcatttt tcaagttcat gatgaaattc ttatcatcag
    14821 taaatgctcc agaaaaataa acaatgattg aaaaatcagt tgaaacttga ccaccgttta
    14881 ttttctccgt attcttagaa tagaagggag tctgattgat gtattcacta atgaaaaaaa
    14941 tcattaccga aaaagatatt cgacgtcatg tttctttagt agaacaattg ctgaatcata
    15001 caaagataac cgtaaatgaa ttagctgaaa tcatagggac aaccgaacga acgatttttt
    15061 ctgaccttca atctattcgc tctcagctgc ccgaaggctg ggacatcatc agcgatcaag
    15121 caggaatcag tttgcaaaac cagcaaaatt tattaacaaa tgacctttgg gagatttttt
    15181 tcaaacaatc agtcagtgtc gaactgctga aaaatctgct ttttacaaaa aaagttgctg
    15241 tccctgattt tctagccgac catgggcttt cttatgggac attgaaaaga catgtgacta
    15301 aaatcaatca gcgtttagca agttacgact tgcagatcga tttgacgaaa tatacagctt
    15361 gtattcttgg aaaagagcgt gcgattcgta ctttttatca tcgattgctg attcctttca
    15421 ctcataacaa ctattttttc gaggattatt cgatccatga atcccattat tttcaatttt
    15481 taagaaacct cagtcaaact gaactagcgg ttgaaacaga agagattttc ggaacatgtt
    15541 ggttctttat caatacgatc cgtataaaag cgaattgccg attagattct tctatccata
    15601 ccaacagcac actctcctcc ctgtatgact ctgcattgaa aaaattgtat ttaacggaag
    15661 gtatttatct taaagacact gagctttctt ttgcttcttt ctgctttctg gaaagttgga
    15721 attacaacaa taactatgga caagaaaccg ctcgctgcct gcatcattct ccatttttag
    15781 aggtcctcga aacgtttgta gaggaactag cttcagagct gtctcttgat caactaaaaa
    15841 aaacatctct gactgataac ttggctctcc tcatattaaa atatcatgaa tcgccgatat
    15901 tgattgaagc attggaccgt caataccatc gttttttaga aacatatgat cagcaatatg
    15961 cacacttgta tcaacagaaa gagaatttac tgaacgatct aaaaaaagaa atagaaatca
    16021 atgaaccaaa ttactttctc caactcttgt ctctactgat ccaacaaacg attttttcta
    16081 tcaaaccgaa tttgttcaat gtctatttta ttttccaagg agaaccttca tggaaggcgt
    16141 tcttgcagca ggaattaaaa gattacttag gaaaacgtgt ctgcttctta cctatcgaac
    16201 taaccgatct ttccactatt tcttttgaaa aaacagatat cttgatttcc aatttccctc
    16261 tagattctct agatatccct attgtataca tttcttctat tccaacgaaa aatgagctca
    16321 atcgtttgac tgaactcact ttcaaaactt ttttatagta acggctagcg actgtattct
    16381 ctagctgttt tttctttttt gcgaaacagt ttgttcatga tccctttttc tatttctaca
    16441 actaagaata gaagaatcac atttgctcca atcaataact gttgtgtcat cgttaaactt
    16501 actgtaccga tcagctgctg gccgactggt agaaaaataa ccatcccttg caataaagca
    16561 aggataccta atgaaagaaa aagcgctttg ttttgcaaaa gaccttggtt caatgacggc
    16621 ttggacaact ctctgcaatt gatcatgtaa acagcttgag ctaagacgat attttgcaga
    16681 agaatggttt gctgtaagct ttgcccttca aactgcatag ccaataaata ggcaggaacc
    16741 atgatcagta gggagacata gatgatccga aacaaatgat aaccagatag gattccttgc
    16801 tttggatctc ttggtgggcg cttcatgatt tctggatctg gcgactcgaa ccctagtgca
    16861 taagataaag tgattgtcgt taccatattg acccataaaa tctgtactgg ggacaaaggt
    16921 agcgggcgat tcagcataag tgcccaaatc aagatcaatc cttgagctaa tgcagttggc
    16981 aagaagaaat tgatggtttt ttgcaaatta tccaaaatcc gccggccctc ttttacggct
    17041 tttgcaatcg tatggaaatt atcatctcct aaaaccatat cagctgcctg tttgcttact
    17101 tcactacctt tgattcccat agcaattcct acatcggatc ttttcaaagc aggtgcatca
    17161 tttacgccgt ctccggtcat tccgacgatt tctccatttt tctgcaaagc tttaacaatt
    17221 cgcagtttat gttctggtgt cgttcgagca aagacatcga ctattggcac ttgctgaatc
    17281 aactcttcat cagacataag atccaaatcg atcccttcta aaactttggc agtatgtttt
    17341 aagccaattt gttccgcaat cgcttttgct gtgtctttgt gatcccctgt aatcattttc
    17401 actgagatcc ctgcttgttg gcattccttt acagctttga ctgcactttc ttttggcgga
    17461 tcgatcaatc cggcgatccc tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc
    17521 tgtttcgaat ctactttttt ataagcaaat cctaaaacac gctgaccttt tctcgcaaat
    17581 tctgcagctg tctgactcca tgcttcttgt tctgcagaag aaagagttga tttagcaaat
    17641 aatacttctg gtgcaccttt gacgtaaatc accgctcctt tttcctccgc atgcatcgtt
    17701 gccatatatt tgtattcaga actgaacgga atctttgctt ctattttttt caaaggtaga
    17761 ttggctgttt ctgtgtgtcg aatcagcgca acttctgtcg gattgccact taaattttct
    17821 gttttctgtt cttcttttgt ttttaattcc tgacagtttt tcataatgga caacacgcag
    17881 gcttcttctt ttgcagctac atccacaact gtcatttcat tttttgtcaa tgttcctgtc
    17941 ttatctgaac agatcactgt catagaacct aatgtttcta ccgaaggaag tcctttgatg
    18001 atcgcctttt cttttgccat ttcatgtact ccaacggata gaatcatggt caaaacagct
    18061 ggcaatcctt cgggtaccat tgacaccacc aaagcaatcg tagccgaaag aaggatatgc
    18121 caatccatac catatcgaaa tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg
    18181 ccttgaaaaa tttgtttgtt taatcggttg atttttttaa tcagaggcgt cgtttgttgt
    18241 tcgaccgatt gcaatgcttg attgatttta ccgatttctg tcgtgtttcc tgtttctaca
    18301 acgacaccga ctgcagaacc agattgtaca agcgttcctg aaaaacccat atttttctgg
    18361 tcgccgatcg attcttcttc tgtcaaaacg gcatgacttt tttcagatgc ttctgattct
    18421 cccgtcaaaa tcgattcttc cactaataaa tcatgtgctt cgattaatcg gatatctgct
    18481 ggaacgacat ctccggcacg taagacaatg acatctcctt ttaccagttc tttcgcaggg
    18541 attttttctt gcatcccgtt tgatagtact atcgcttctt gtcccatcag ctgcttcaaa
    18601 ccattcaatg attcttccgc ttttctttct tgccagtaac taacgagtcc attgacgatc
    18661 actacgaata aaataatact tccttcaata tattcactag aagcaaattt caaaaaagag
    18721 gcaatgatca acacgatcat cagcagatca gtaaaatgct ttgctatttt tttccatcgt
    18781 tttgtctgct gccttgcttt tatttcatta tatccttgat cagtcaggcg tttctgtcgt
    18841 tcttcttgcg acaagccttc tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc
    18901 aatctgtacc agttcatcct atcccctcct gtttaattga tactgttatt atacgcaggg
    18961 aagttttttc aggcgtatcg attatttcag gggggtctga aataattcgt tttgcaaaat
    19021 gtatctgggc ctaactgata tttctattgc ttattctttt atatacaaaa tttaagaata
    19081 caaaccagat aaccaataaa aaaggaataa agcgtgtcgt gctttcgaat aacaagctga
    19141 aagcaattga agtaaaaaac accaacaaag caagatcttt atatctttgt gtagtgttcc
    19201 gtttcttttc tttaataaat tgaacatgtg cacacaagat cgaagaccac acaaatagaa
    19261 aacaactcgt tgtcatgcct gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa
    19321 aatgtagaca aagaccggca aaaaataaca ttcctgtaat taaaataccg tgtaaaggta
    19381 cgcctttctt agaaagcgca caaaaccaat ggggcaaact tttttctttt gctgtttgat
    19441 ataaaatcct gctagtactg tagactgcac tatttgctga ggacaaagaa gcaaccaaaa
    19501 tcattagatt gacaattgct gctccgcctt ggcaatggat cacttgcagc atagtcacta
    19561 aaggactttc ttttaaatct aaacaatgcc atggaacaac aaataaaatc gacaaaatcg
    19621 ttcctatata gaaaaagaga atacgccaag gcagacagtt gattgctttt ttcaaattct
    19681 tttgcggatc acttgtctcg ccaacggtta agccgatcat ttcaattcca gcaaaactga
    19741 ataaaaccat aggaaatgag actaccattc cagcaaagcc aaacggtagt aattcatcga
    19801 tctgtccaaa attttctttg attccgatag actgggtatg aaaatctgag acactcaatc
    19861 ctatacccaa taagataaaa aggaaaatcc ccaagatctt gatgattgag aaaaaagatt
    19921 ccaattcacc aaaccacttg actgcaacaa ggttgactgc cattaagatc attagtgcgc
    19981 tacctccgct tatcaaagct ggtacatgta caaaccagaa atggatatac aatcctattg
    20041 ctgttaattc cgcaagtcca gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa
    20101 atgcccactt tttgcctaaa taatagtcaa cgaaatcact gatagagtta aatgtactat
    20161 ttgctaaaaa taaatcaccc atggcccgca tcaaaccata aatagccaaa ccgcatatca
    20221 aataaacgag aataacagct ggccctgctg atgcaattgc ctttgctgaa cctaaaaata
    20281 atcctgttcc aattgcgcca cctaatgcga ttaactggat atttcgatta gttagagtcc
    20341 gtttcattgc cggtttttcc atcaagttca cctttttctt tttcaagcag catggcgact
    20401 tcctgttccc aagaaggttt ctttattttt tcaatacaga gtggtactac aaacaaaatg
    20461 acactgatgg ctgtgactgc aattagattg atccatgttc cagctttcat cgtcagtccg
    20521 acagaaaatc cgattgccgc aaataaaaca ataacgacag tccaaactcc aagattgctc
    20581 tttttctctt tttttccaat tctaaatggt cgattcaact ttgcatgtgt ataacgcaat
    20641 ctcaaaactg cgatccccat gatgacatag acgatacagt agataacagt agttgaattt
    20701 acgagcatca aaaatgcttg attgacgcca ggaatcagca aataaaaaag tgcaaagatt
    20761 gagataatga cagactgagt caaaataacg gatttggata cgtcaaattg attcgtcttc
    20821 caaaaattga attttggtgg atacgcacct tttctagcac tttctgtcac tgtctttgct
    20881 ggtcctgagg cccaagcaga taattggact gctacaccaa tgaagacaag taaactaaac
    20941 agattcacaa taagatgtgg caaaccaaga atttggcaat aaatcgaaat agattgcgcg
    21001 atattattca attccatttg tccttttggt acgacatttg ctacaactaa agcattcaaa
    21061 atattgacga aaaatataaa aattaacgcg gcaaatactc cgcgaatata cgttttaact
    21121 ggattttcca atcgagtgat atacacagag gacatttcga taccagtaaa aataaacatg
    21181 atcggcgcaa aataaacaaa tgaggatgca gtcgtagtat ctggtattaa tttgtcccat
    21241 gaaaaactgc ctaatgttcc ggttggtacg atcccaactt tgatccacgc agcaaaccct
    21301 aataagagca tcatcgtcaa aggcacgtac aatcctaacc aaacaccgac ttttccgccg
    21361 atttttgcca tatcaaattt caaattcaaa aaagtgatga tccaataaac gactaaaatg
    21421 acaactaatg taaatttgct gttcaaacca agcggcacat tatcgataat attaccaaac
    21481 aacggagcga gtgctgaagc gaccatcacc atcccaggaa acatttggac ccataacagc
    21541 catgagacaa cgaaccccca tttttttcct agagcattcg atacccaaag ttctggacca
    21601 cctttttggg gaaacattcc agcaaattca ccagagatca atgagattgg aaaagcgtac
    21661 aataatactg caaccagcat gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga
    21721 atatttcgta cacttgctac aagggcgcag gtcattccga taaaggtcat ggtcgtgatt
    21781 tgctttgatg aaccagtatc tttcatcttt attccttctt tcaatagcga catatgtttt
    21841 tgttttcttt tatttttgtt caagaacaga aatgacaagg tttcatcgat tcagtacaaa
    21901 acccagtatc accgaaacat ccattgaacg atcttagtga actgggtttg atacatgaaa
    21961 cgaacgatcc aacttataga acgttttata aaaaagaata tcttggcgag gaatatatga
    22021 aaaacagggg gaaatacaat tgttttgatg aagcaaataa ttgtctgttc tccatatgtc
    22081 tcttgttatt ctttcacaca aaaatcagtt ctattggatg atttcgttta gtttcttttc
    22141 cattgctttg gcgatttttt ttgtataaaa attgaaaatt cgatcatcat tcaaataagg
    22201 agtcatgata gccgcacgta gcagagtgat tttctgttct tttttccatt cttctttcgt
    22261 tagacccatt ctttcaacaa aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt
    22321 agtatgagaa gtaataaacc gttcaccata aacatcgcca tccatataag aagaccgatc
    22381 gaacattttt tcatttaatt cattgatagc gttcaaatct gtacagcctt gttctttgaa
    22441 tacccaattg accatattaa aatcaggatg attcaatgga tagacttcga ttgtttttcc
    22501 tttaacagta aaagtcagat gatcgagaaa ttctctgaat ctttgagctg cctcgattga
    22561 agccccaatc aattgaccat atccggtcac attcaatggc aatacacgat gtgctgtcca
    22621 gactgcggcg gcggttgctc cagcttttga tccttccaaa atataagctc ctaacatatc
    22681 tggagccttt actgattttt caaacacata tggcgcgaaa taagaaatga tatttctcat
    22741 atttttatgt ttgattacga ttcctccagc agcataaggg acatagccca tcttatgagg
    22801 atctatcgtg acagaatccg cttctgaaat agctctgaac ccttcatata cctctttgtc
    22861 aatcgttacg cagtggtgga aaacatgatg ttcttcaaag aattcagcta aggaagcata
    22921 cggcacaaat tctcctgctt cgtttagaaa cagtgaacgc gcatagccgc catatgcggc
    22981 atcaacatgt aaatagaaat agatcccttc atcttttaac ctttctctta attggacgat
    23041 tttatctaca ctgtctactt gaccttcttc tgtcgttcct acgacagcaa ccacacctag
    23101 aattgggatt ttttgtccag ctaattcacg aattgttttt tccaaagcat taatatccat
    23161 tcgataatcc tcttgaactg ggatagcaac catctgatct aagccgactc cgcaaatatc
    23221 taacgccttc atccaagaat agtgttttgt ttgagggacc agccatttcc ctaatctttg
    23281 gatatttttt ccgctacggg aagaagctgc tttgacctca tccatttctt cgtccgtaaa
    23341 gctttcggtc atttccaaaa tttcttcaac agataaattc aaaagttccc actcagacat
    23401 ttttttcact ttttcaggca gcacttcctt gacagctaaa ggaatcgatt tgatgcaacg
    23461 ggcgtaccat aatccttcaa gattagcaat agaaccatct gctgcaatgt gtccccatcc
    23521 gtctgtcata ttgaataatg aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt
    23581 tgccatagaa gattccaatg caacattatt tgggttccac agcatcgcat agttgtaagc
    23641 taataaagca ggcatcaatg tttcagcatt catttgtccc cagtaacggc ctgctgaatg
    23701 ccatgggatc gatcctgcgc gtaatcgctg actgacttca tccaagacct ctatcatatg
    23761 gtcgcgggtg gctagataat ctggagtgag cttatctcct tcgctaattg caggcatatc
    23821 tgaaggaata taattttctc tccatccaag atgttcatct accatcttgt tcaacaacat
    23881 tttataaact ggtccatttt cagctttgtc gcctataaag acggccttga tatccatatc
    23941 tttcattttt cttccctcca tattttgttg gtcaatgtct tgcagataca tgtgtttatc
    24001 tgtaacacct cttcactata gcgccaaaaa tgagaatttt ttttttataa catcaaatcc
    24061 acgtagattt ttacaaacaa aaataaataa agtcggattt ttctgataat tcaacgcaaa
    24121 aaagcgaaca atttttcaat aaatcagtct tctaaaatag ataacctaaa aaaagcgaac
    24181 aaacaaataa aatgactgtc cttcattcac taaacatatt tttatttttt tatattgtta
    24241 aaaattcgca atttattttt taatagaatt atctgttttc caaattttat aacataaaaa
    24301 acctttttta acggcattta tatgcttttt cagaaattaa ctgatgattt caaaaagaaa
    24361 acatgtcata atatttccca tattgtgcaa aaaaatacaa ggcggaggct ctactatgat
    24421 tgaagaatat attgagaaaa atattttaag acagctgttt ttatgtgggc aattctatgt
    24481 aaataaagaa gtcaatctag aaaagctctc caatctcctg catgtttgca aaaccacttt
    24541 actcaatgat atcaataaca taaaaaaaga atttgaagaa cagatcgctt atacacatcg
    24601 ggaaaaggac cgttacactt tatatttttc agaacatata cctcgatgca agatcatgca
    24661 gcaattgtct caaaattcgt tgtttttaaa aacttgtctg ctttatttgg aagaagatga
    24721 acctgattat cttcaattaa ctgaatgtga attcatctct gtcagcaaag cttattcatt
    24781 aaaaaaacaa gtattagctt attttaatga ctgcgggatc gaaatcgacc gctactcccc
    24841 tcgattcact gaaatggaaa gacgtctgct gcttcttaat gtgtcgtatc gtttaggcgg
    24901 ttttaattct tgggagctac ctgaatcgtt ttttgaacgt gccgatcgtt tcattgaatc
    24961 cgtaacggaa aatagtggca gattttatga taaagaaaat aaagaaatct tatcgattgg
    25021 ttttgctatc agtttcctac gccagcaagt gtgtgctgtg acaatagatt caaaatttat
    25081 cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa tcagcatggg aaaacactga
    25141 tttccaaacc tattataaaa aagaagaatt cgcctttatt ttgacactat tcaatctttg
    25201 taattatggt ttccattctt atcagttgat tgcggaagac tttcaacagc tacaccaagt
    25261 gtttattgac aatactcccg aaataaaaga actagttgct acctttgaat ctcacttcaa
    25321 tcaagaatta ttcggaaacc aaccgttcga acgtgcgctg attcatctga tgcgttcggc
    25381 ttgggataac tatcaattat tcatgccaga aaaattttat cttttaaatg aggaacagac
    25441 aaatttatat aaagaagtcc agacaatttt ttccagctgg tccagccagt taccttatga
    25501 tctacggttg aatcctaatt gcatgcgggc ttttgtgatc gaattgagcg gaattctccg
    25561 tctgacaaaa gaacatctga ctatctatat cgtcacaaat tcggatgttc attatctgat
    25621 ttatcgggaa gccttggaag ctgtaactac tttcgatttt caagttgcac caacaattta
    25681 ttcaagtata tctgatataa agaagtatgc gcagcagtcc tcaaaccggg tattatgtga
    25741 acgtacatta tatacaccag atgcggtcca atatgaaaat attattccta tttcgatcaa
    25801 tacgatcgat cgagctatca tttcagctgt gcaaaacaaa taaggggctg tgacaaaaat
    25861 catgtcacag cctcttattt gttgaagatg cccatttctg taacaatctc ttgatacacg
    25921 tcaggctcac aacctcactt attttggaca tctacgggtc cgatctcttt atttaaaaaa
    25981 taagaaatga tggttcggat aactaccaac gccgctagtt ggaaaatatc ctgcaaggta
    26041 ggtttgataa tcgattcgat gatcccagct gaaacaagaa tttccaaact tagaagtata
    26101 tagcctccca acatcttttt aatagcattg ttctcgctga ctcggtcgct ttgcactttt
    26161 gtatggacaa ataaatattt caaaaaatcc ttgattgata gacaaacacc ccaaaccaat
    26221 aaacagatag caaagatatc aagtcccagt acaagttttt cgaaatatgg aagaagaaac
    26281 tgcatcaact ctgtcgtcat cctacctctc ctcttctttg tttttctttg tcttctattt
    26341 cctgattcaa gaaataagaa atgactgttc gaatgataat aataagtgca agcttcaaaa
    26401 tatctgtcca agtcggcttg ataactgact cgatgatatc agcaacgact agtacttcca
    26461 agcttaacaa aatataactt gctagcagtt ttcttgtatc tgcattacga gtattacgtt
    26521 ctgcatagtt tttcttgaca tgagaccaat caaaaagttt gaatagaaca agaacgatcc
    26581 cgacaattaa gataccgata gaacaaatat ttaaaatagc aatgatccat tctagaaatt
    26641 caattaaatt tgagcgaaac atatacgcta cctactttct tttaatttgt tcctattctt
    26701 ttattgtaaa agaaagttac cttgttcgtc tatttaaaag ttgttctaat gataacggtt
    26761 cgcttaaatt tttctttttc actctcccat ttttctccct ccatccataa atcgtggaag
    26821 tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc gtcatcagct tttcaaagaa
    26881 gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa atagaaccat acacctcctc
    26941 aaatcttttc ttcttaccta ccctattttt atttacagga aaatcagaca cttcttcttt
    27001 tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg cattctaata aaaacgaact
    27061 atccttggat atagaaagag aaaacaagga ggcgtttttc atgagtaaaa aattaatcaa
    27121 agtcggcatc ggtttaggtt tgcttgccct tggcgctgca tatctaggaa aaaagacagg
    27181 actattcgaa gatgacagtc atttatatga tgaattcgaa tcaatctagc atcctattta
    27241 gttgaatcac tacggacata cttggaagac attggaggaa tcacaatgag aaaaagtaaa
    27301 gcaataatga ttggtgccgg tattgccaat atggcagcgg ctgtttattt gattcaagaa
    27361 ggcaaatgga aaggggatca gatcaccttt tattcattgg atgatcacgg ttcaaatgat
    27421 ggtgcaccga ccgacactgt agtggatgaa tattggaata aaaaccatcc attagaaaac
    27481 caaaaaggct atattgctcg aggcggtcgc atgcttaatt accgtaccta tgtagatttg
    27541 atggatctgt taagtcgaat accttctgtt actgagccag ggatgactgc tgaagaagat
    27601 acgagagatt ttgatgcaaa acaccgaacc tttgacaaag cacgtttgat ggaaggtggc
    27661 aaaggaatca ttgatggcgg aaaactggga ttcaataata aagaccgtat tcttctgact
    27721 aaactgattg ctatgcctga ttcagaagaa gaaaaactag acaatatcac tatcgaagaa
    27781 tatttcaaag atgatccaca cttttttgaa acaaacttct ggtttatgtg ggaaacaaca
    27841 tttgctttca gaacacgtag ttctgctcaa gaattgcgtc gctatatgca tcaaatgatt
    27901 tatgaattca ctcaaatcga acatctagtg ggcgtcaatc gaacacgtta caatcaatac
    27961 gaaagtatta tgcttccttt gatcgagtac ttgaaagaac aaggatgcaa aatcattttg
    28021 aaccgccgtg tcactgactg ggaattcaaa gaaacaccga tgcaagatga aatcactgtg
    28081 actggtttga aaattaccaa tacactgact caagaagaag aatacgttgt ggtcgatgaa
    28141 gatacagcag ttattttcac taatgggtcc attacagatt cagctacttt aggagattac
    28201 gaaacaccag ctgtagaaaa tatggattat ggtgcagctt ctagtctctg gaaaaaagcc
    28261 agtgaacgtt tctacaactt gggaaatcct gacaaattct ttgctgatcg agatgcaagc
    28321 gaatgggtca gctttaccct gacgacgaaa aaccatttgt tgttgaatga aatcacccgt
    28381 atcacgactc aagaaccagg aaatgccttg aattcattta tttcaaccac accgatcacg
    28441 ccgttaggcc aaaaagatgt caatatgtct atcgtcgtgc atcatcaacc gcattttacg
    28501 tctcagaaac caaatgaaac agttatttgg ggttatttcc tttatccaag acgacgtggc
    28561 gaatttgtgg ataaacagta tattaaaatg aatggaaaag aaatgctaga agaactgatt
    28621 ggacaacttt caaaagtaga tcctggacca gtgaatattc gtgaaaaaga aacagaaatt
    28681 ttcgacagcg taataaataa tatccctgtc tatatgcctt atgcttccgc gttgttcaat
    28741 aatcgtgcca aaagcgatcg accaaaagtc attcctaaac attcgaccaa tctggccttt
    28801 acaggagaat ttgtcgagca gccttatcag atgatcttta ctgaacaaag tgctgtacgt
    28861 tctggagaaa tcgctgcttt ccattttgcg ggtattccta tgtcccgtct tgtcaaaaca
    28921 ccgcgttacg acaaagatat tcctactcta gctcgtgccg caaaaaaaat gtttgaataa
    28981 tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacaaccag gctaaaacag
    29041 cttcttatgt ttttgatttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
    29101 ttaccaagag gttgtgaaaa agctgccctg catcaagtaa taagaacacc caagctaaaa
    29161 ctgcttctta tgtttttgac ttggttggac ttattaccgc agatgcaagc ttttgaacac
    29221 catttaccaa gaggttatga atgccctgca tcaagtaata agagcagcca agctaaaaca
    29281 gcttcttatg tttttgactt ggttagactt attaccgcag atgcaagctt ttgaacaccg
    29341 tttaccaaga ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa
    29401 atggttcctc acgtttttgc ataaatttga cttaataccg agaaactgat cccttctgtc
    29461 ataacctctt tttatttatg tctgtttctt tttgaaaaca actatttttt caacgtttac
    29521 tctcttgcca atccaacatc atctaacgga tacaactatt tcaatgatta ccattacact
    29581 aaaaaatcgg aaacacacct ctccgaacac aaatacataa gactatagaa tgtttcattt
    29641 aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc tttaaaatga aattatctgt
    29701 agtactaata atgcagacaa ttttatgaat ctgaaaggag catctccaat gaaagcagtc
    29761 acttggcaag gaaaacagaa aatggaaatc agaaacgtag atgatccaaa aatattagaa
    29821 ccaacagatg caatcatccg tatcacagca acagccattt gcggctccga tctgcatctt
    29881 taccatcatg gtgaagcagt tttggaaaaa gggtatgttg taggacacga accaatggga
    29941 atcgttgaag aagtgggatc tcaagttcaa acattgaaaa aagggcaacg tgtggttatc
    30001 ccttttaata ttagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt
    30061 gatgaatcaa atccagatca gttatatggc gggcttttcg gatttggcaa gttgaacggt
    30121 aatcattggg gtggtcaagc ggaatatcta agagttcctt atgcagactc tacttcattt
    30181 gttgtaccag ataatgatct tcctgatgaa aaagttttat ttctatcaga tattttgccc
    30241 acagcttatt ggagcgttga aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta
    30301 ggttctggtc cagtcggtct attcgctcaa aaattcgctg ttatggctgg agcagagcgc
    30361 gtaatcgcag tagatccagt acagcaccgt ctagataaag cggctaatta caatggggtt
    30421 gaaacttatt tcttagaaga cacggcacaa gctggaacag aattgtatga actggctaaa
    30481 ggtggcgcag atgtcattat cgattgtgta ggaatggacg gattagagcc tgtcaaagaa
    30541 aaagcgaaaa atttagtaag cttacaaagc ggcacaatct ctccattaca gatggcatcg
    30601 caagctgtca aaaaatttgg aacagtccaa gtaactggtg tttatatgac tccagcttct
    30661 tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg tcaagcatgg acaggcacca
    30721 attatccatc taatgccaaa aatttacgca atgattgcag aaggactatt cgatcctaca
    30781 caaatcatta ctcattcgct gccactatca gaagcagcac aagcatatga gatctttgac
    30841 aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt gaattcagta aaaaatacag
    30901 gtgattttct ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa
    30961 gaagatatcg aaatacgagg acgtcgcttc cgcttctatt tccaaaatcg ttacacattg
    31021 atctcactgg ctgtcgattt attaaccggg attttctata tttttggaag ccttgcttcc
    31081 ttaactaaaa tccccgatcg atatggcacc tatttttatt tggctggtgc tattttcttg
    31141 acgattcgtc ctattctcag aatcgtaagg aacattttta tttacgacga aaaaaaagca
    31201 atgaagagaa caagcaaaaa aaaagaggca tgagaatttt tcaaatctca tgcctctttt
    31261 ttcaccctat cgtatcactt tgatacagac gagcggtcgt cccactttct tccttgacca
    31321 ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt atgatctgta attaagtaaa
    31381 gtttttcgta cccaagttga tgcatttctt ttctgatttc cgctaatatc cgcttggcta
    31441 tcccctgctt acgcttttcc ggttcaacaa acagggcaca aagatttggt gtcagatcag
    31501 ggcggttatg gaagtcgttt tcaatgacgc ccgctcctcc gacgatggtt tctttttcat
    31561 ctaagaccac ataccattga ggaatacttt ccttattagc gattccagta cgaatacttt
    31621 cctcatacac aacagcagga actccccatt ttgatgaaaa ccaattcgcg ctttgtttta
    31681 ctaattctgg gtgttctctt aatttgatcc agttatatgt aaccaatgtt taaacctccg
    31741 actaaaataa aacgtttaca aaaattatac aagtctttat gaaaattagc aatgacaaca
    31801 atagtgatgt aaaaaaaaaa cggaaaacaa tcacttgttc tccgtttttt gattgatcaa
    31861 gcatctaaag cttcttttct tctttcttgg atcgtagcta ccttgacgaa cggtagatag
    31921 atcaaaatag aaagaaccaa attgacagcg gctagtacac cacctgcaat actttttgtt
    31981 gccaatacac cgccaataat tggtggagtt acccatggag gcataacggt agctgctggc
    32041 actaaacctg tagaagtcgc aaagtaagca acactaacta agaccattgg tgtcaaaata
    32101 aatggaatga acatgatcgg gttcaacacg ataggtaacc caaacatcgt tggttcatta
    32161 atattgaaga cacctggcgc aatcgacaag tttgttacaa ccatatatgg tttgttttta
    32221 cgtcctacta aataaatagc taacagtaaa cctaaagttg cacctgttcc tcctaagttg
    32281 acaaagctgt caaagaaagg tttgttcacg ataaacggtg cgctatgccc agctgcgatt
    32341 gcattaacgt ttgcttcaat ggctggcgca ttgattgttt gcatcaaagg atcgaccata
    32401 ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga taaatgcaag cagtaaggca
    32461 gatggataag agtttgcaag tcccataaat ggttcttgga ccactgtata gaaagaacct
    32521 acgatatcat caacaccgaa tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata
    32581 gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca
    32641 ggcattttga tgatcaattt ttcatttccg cttaaacgta caaagatttc tgttgaaata
    32701 atcccaacaa taatagcaat aaacaaaccg tttgagctca tacctaatgc tccgcctaaa
    32761 gcgaagaaag agcctaaaga aatcacacct gcggaaacac cgtcttttcg gtagccgtta
    32821 gctaaatgat aagcaagcaa gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc
    32881 caagcagcag ccccgaatcc tttccatgat tcaccaccaa aaattgagtt catcaattct
    32941 tggaacccgg gaatcggtaa gttattgatc attactgcta atgctcctaa gatcattaac
    33001 ggcatactca ccataaatga gtcccgaatc gcaactaaat gccgttgtgc accaattttt
    33061 gatgctacag gtataaaatg tttctccata aatgcaacaa atttatccat aaatcttccc
    33121 tcttttcttt actgtagatt acgcgttcaa tcgacgatac agatcaatga tctcttttgc
    33181 taaatctgta aaagctatag atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt
    33241 gacttcaacg tgatttcctt gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc
    33301 ttggactaaa gattcctctg catctttgat tttttcatct gctaaagtga aatcaccttt
    33361 tttcgcagca gcaatcgctt ccattgcatc gctttttgca tttccgccaa acatgatcag
    33421 acccatgatg gcttctagat ttttttgatc ttccatttat tgtatcctcc gataattttt
    33481 attatttgtt tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat
    33541 agtcagccat attgattaca tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt
    33601 gattttgcat aaagcgtact tgcggtccta aaagtaaaac gtctacattt ttagattcta
    33661 gttgattatc cgcatctgaa gcagatacag caaagatgtc tgcttccatt ccttttgctt
    33721 ctgctgcctt ttgcattttt gtgactagga gacttgtact cattccagct gaacatacaa
    33781 gcatgattgt tttcttttcc attttaaaca cgttcctttc tatacttaca cttgtatata
    33841 agcaagtagc gtgccaactt taaaagcgct ttaaaaagtt gatatcatca catttaaggt
    33901 ctataccaag ttgcatacac actaaaaaag aaagcgcaca ctaaacacac aaattaattt
    33961 ttattacctt ttttctttta acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac
    34021 ttttttcaaa taaacggtat tcaaaagctt gcatctgcgg taataagtcc aaccaagtta
    34081 aaaacataag atgttgtttt tactttgcta ttcttattac ttgatgcagg acagcttttt
    34141 cacaacctct caataaacgg tgttcaatca gctgaccctg ggtattagct caaaacgctg
    34201 gattcacaac ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact
    34261 tctatttatt tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga
    34321 taccacgaag cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact
    34381 aatccatatc gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac
    34441 atatagcctt cgcaatttgc gccttcttct gttgctttga tcaagtaatg caaatgctgt
    34501 ccgataaatt cgatacggta atcatcttgc acgattcctt tctcatccat atactgttct
    34561 tcatattctc ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct
    34621 tttagataca tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta
    34681 ttcattttct tcccaggtaa atcaaagact tcataatatc gttcaggatg gaaaggtgtc
    34741 tcttcattcc attgataacg aggggcgcga acgcgtttag gataatattg attgattcca
    34801 aggaaatcta ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa
    34861 atcgattgtt tttgacacaa ctccaggaac tcttctgtgt agtatccttt taccatcgga
    34921 tcaaaaaata ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga
    34981 gaattggatc gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa
    35041 tttccgctgc ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac
    35101 atcatccact tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc
    35161 gtttggggaa cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga
    35221 tcaaaggcaa tttgagcata cttagcataa aaatcaacta cttttcttga agaccagcca
    35281 tcatattttt ccaaaagaaa agctgggact tcataatgtt ccaaacaaat catcggttct
    35341 acgtctgctt cgatcaaaga gtcgatcatt tcacttatat gctgagcata ttcttcatct
    35401 actatacagt tctcataatc agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt
    35461 gtcagatgga tggatttcat cagatccaca tcctgctggt attgttccat aaaattagtt
    35521 gctacggctg ggccataccc attatgccaa acaaaatgtt catttttata ccagctatct
    35581 ataaacgaat cctgtccttc tttttttcct ttccaacctt ccgtttgcca agcagaagat
    35641 gcagcaccaa gaataaaatt ttttggaata gtaattttca tgatttcctc ctgtttttta
    35701 gatcccatca ttttccacac gatttgctac ttttacaaat ggcagataga tcaaaataga
    35761 aacaataata caaacaagtt gcgtaataac tgcaccgata tttcctgctg ttgccagata
    35821 agcattgacg atcggcggca ttggccaagg gaccattaca actgctttcg ccgcaaatcc
    35881 taaagcggtt gcaaaataac caatcgttcc agtgataaga ggcgttaaga taaacggaat
    35941 agctaaaatt ggattcagca tgatcggcat accaaaaata actggttcat tgatgttgaa
    36001 gatcccagga gcaagggata acttggtgat ttgtttcata tcttcccgtt ttccaactaa
    36061 caatactgca atcagtaaac cgatcgtgac tccagacccg cccatactca tatacatatc
    36121 ccaaaatggc atagtaataa tgttcggtac ttcttttcca gcttcaaaag cttccgtatt
    36181 tacagcaatt gccgctaaaa gcaacggttc ccgtatcggc ttcaccattt gatttccatg
    36241 gataccaatc acccaaaaga cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc
    36301 ttgtacgaca ccttctaatg ggcgttgtac gacattgtaa atgatatcat aaaggtacat
    36361 tcctgtaagt tctttgatga taaatcccgc agccgcgata cccgatacag tcagaatcgt
    36421 cgggaataaa gcagaaaaac tagcagaaac attcgttggc acggtatcag gcattctcag
    36481 ctgtagtttc ttctgtttcc tcaaccagca ataaagttca atagaaagga tggagaccaa
    36541 cattcccaaa aataaccctt tcgtatctgt atattgttta gccaatacgt tttctactat
    36601 aacagtctga ttttcattca ctaattcata aacagttgga ttaacagata aataagacat
    36661 cactgcgatg ataccaggga atacaccatc cactttattc agcttggcta attcgatccc
    36721 tatcagaaaa acagcataga tggtcaagaa acttagtgtg acatagctga tggcagatgc
    36781 gataggcttt aattcttcta aaaaacgcaa gaaacttatc tgtgctaagc cattttccga
    36841 gtcaaagacc attgctgaaa acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa
    36901 tgcagcaaat gcgtttttta tcaccatgat ataccttaaa ttattgattc tcgttgccac
    36961 gttccctaaa acattggcta atttatccat aaaactcatt ccagatcccc tcattcctgc
    37021 ttcttctcct tgacgtcatc tctttttgtc tattagtcag cgcttacatt tcgatgaaca
    37081 gaaactattt actcctcttt tttaccaaat ataaaacaaa aattcatctg atgtatttat
    37141 cataaaatac atcagatgaa ctgtcaatta gtttcttttt ctcctattat tttctgtaat
    37201 tctctccgat ttattgccat atggacagtc attgcatcaa acgcagcaag tgcattccgc
    37261 ctttcgatgg cctgaacgat ctctgtatga atatcgatcg tctcgatttt cagatgctgg
    37321 atatcataat ttttattaaa caaagcaatt gattcattaa tgattgggat aatatgcagc
    37381 aaggctacgt ttccgctagc ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca
    37441 tgttcttgtg tgccttctcg aaaagaagtt tcaatctttt gcgttaattt tttcatttgg
    37501 acgatttgat tcttcgttgc atattccgct gccaaacctg ccatcttagg ctccaataaa
    37561 tatctcagtt caaataagtc tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg
    37621 agtgggtcat tggcaatccc tcgcttatca ctgacaaatg tccccgctcc ttgccgaact
    37681 tcaaggatat tcctggaagc tagagcccgc acagcttctc gcaccgtgct tcgccccacc
    37741 tctaaataat cggacaattc atattcattc ggcagtttat ctcctacttg caactgcatt
    37801 tcctttaaat atctaaggat cttgtcagta gttacttcta ctagggtttt attttcttcc
    37861 aaaccacata cctccttttc atctgatgta ttttttcgta attattataa cacgtttgaa
    37921 tcatctgatg attttttgta tacaaaattg attaatccgt tcatagacat acaaacgaag
    37981 aagatattta ccataaaaaa gactaggata aaattcactc ctagccttcg atcattttta
    38041 ttttgaacga caaaaaatat gatgttcaat aacagacgat cttcattcga ttcttgatgt
    38101 catcttttgt tttttttaat atatggataa aaagtttgat tacatcgttt ctattctaga
    38161 atttggtcga ttctcaattc ttcttctggt gagaaatcta aattatccat cattcgaaca
    38221 ctttcttgta actgacttaa ccgacttgct ccgaccagta cacttgtaac agatttttgt
    38281 cgcaaattcc atgcaagggc catttgcgct aatgattgtc ctcgtgatac ggcgattgtt
    38341 tgcagtgctt tgattttttc cagagttgat ccaacttgtt cttctgaaag gaatggtatt
    38401 tccttacgat gtgctctaga atcttcagga ataccatgta gataacgatt tgtcagcaac
    38461 ccttgagcaa gcggactgaa cgtgattgcc cctagccctt cttcttctaa tactggagat
    38521 agatcgtctt ctagtagtgc acgagaaaac atattgtagc gcatctgatg aataatgaac
    38581 ggtgcttcct ttctttttaa aatttcagtc atttttttgt atcttctcca ttatagttgg
    38641 atataccaat atataatgct tttccttgtc ttacaagttg catcaaagct tctgctgttt
    38701 cttccaaagg tgtatcaggg tctgggcgat gatgatagaa gatgtcaaca taatctagcc
    38761 ccattctttg gagactttgg tcacaacttg aaatgatgga tttacgagag ccccattccc
    38821 catatggccc ttcccacata tgataacctg ctttgctaga aataatcagt tcatcacggt
    38881 aggcttgaaa atcttctcta aaaatacgcc caaagttttt ctctgcagct ccagcagggc
    38941 ctccataatt attcgcaagg tcgaaatggg taattcccat gtcgaatgcg cctcttaaaa
    39001 tctctctttg attatggata ggatcataat caccaaaatt ttgccataat ccgagagata
    39061 aaatcggtaa tttcaaacct gaatgaccgg atttacgata ttccattttt tcataacgtg
    39121 cactatctgc aatatacaat ttttctcctc ctcatcaaat ctttttaaaa cgctttcttt
    39181 tttctatttt tagtttacta cattttaaag aaaaggagtt aatcaaaatg attttttcgt
    39241 atattttttt gccatttgtt ttttactcgt tgatagatca gaaattgatt acgtgcttgc
    39301 tggatttttt tataacgttt cggttgtttt ttgtaaaatt gctgatgata gttttctgct
    39361 ggccaaaacg taccggctgg ttcgattttt gtgacgattg gttggtggaa agttcctgaa
    39421 ctgtctagtt tctgttttga ttgttccgct aattcttttt gtcgttcgcc ttgataaaaa
    39481 atcactggac gatattgctt ccctctatct tgaaattgtc caaatgtatc tgtgggatca
    39541 gtaatttgcc aatagattgt cagcaactcg ctataactta tttctcttgt gtcataaatg
    39601 atctccacag cttccacatg acctgtgtaa ccaccactta cttgatcata atttggtttt
    39661 tcaacatgtc cgcccgtgta tcctgacaaa acagatagga tccctttttt cgattcaaac
    39721 ggctcgacca tacaccagaa acatccccct gcaaatactg cacgttcttg atagtcagca
    39781 tcttgcatgg catgcttgga aaagtcatat ttttgctcct tatcaaatcc ttccgtaatc
    39841 aataaataaa agtccattac atcaggcgtc agattcattc ggatagctaa tggacgtagt
    39901 gttgcttcta gttttgataa ctgttcttta acggatatat tttctcttat gtcattcttt
    39961 gtctgaatca atacttttcg ttcccaatct cgtgtattcg gattcaaaat cagattataa
    40021 agtgtttgaa tgaatatttg ttcctcatcc atagattctc ctcctttccg aagaagacaa
    40081 gctagtgact ttagctgtaa tttatcataa agtgaggaaa taagcgatta cttcatcccc
    40141 ctacagattt ttcgtataaa aaagagagaa tccttatcga cagattctcc tttttaatca
    40201 atttatttta taaatgaaaa gttaatttac cttttatgga aaactatttt aaaaaataaa
    40261 cataaatatg cagttaatgt aacaagataa gaaataatag tcgttactgt ataacttgtg
    40321 cttactttaa tatcagcaac tcctttgact ccgcttattt gtaccaatcc attagcattc
    40381 acctgctcct ttacaggttg atgatttatt tctacattcg ttcctaaata agcatataca
    40441 ggaagtgtga ttacttggct cttctcatta gttactctat atatgataga actaccagtt
    40501 gttttataag gaaggtttac ccattgactg ccaatctttg cttcgtgttt tattatatgc
    40561 tccttattct ccaatgaagc gataggagaa taatcatata aaaaataatc cattccttta
    40621 gtggtatacg cctctactgt ttctttgttt acaacaatat gtcccggctg agaaattatt
    40681 ttttgattga aatttttttt actagaaaca gttaaagtga ttaataacaa aacagtcata
    40741 aataaataaa atagttctct tgtcttattg tatcttctat ttttgaaaaa tatcatcgtt
    40801 aatatcagag aagagaatat tatttgcaag cttaagatcc gccaaggaaa ttgtaacaat
    40861 ttggctggcg tattttgcag aagataccat ggcaccaaac tagttgtagc tagccaagtc
    40921 aaacacgtac cataataaat ctttttttct atgtttgtta attttttaaa taaaatcagg
    40981 ggaatacata aagataacag accaactagt cccattgagt aagtagttaa gtcgttgttc
    41041 atagcttcag tcagactatc gaacacatta gatgcatgag catataaatt tgtcacgcta
    41101 ggtttgttga ttttttggta gagcatttgt tcaaacatcg gataccaaaa agcactcgtc
    41161 agaaccaatg tcattcctgc cgcactaaat aattttttaa atatagcaat aaaatctgca
    41221 ttcttgaaac ctttagaaac caaacgaata aaaatgataa ttaatgctgt actcattaat
    41281 gttgatagta catggctgta cactagcaaa ctcatactca atgctagctt tacagacgga
    41341 tacttttctt cttttccaga gatgatttga taagcataaa ataatacagg gataacaagt
    41401 gttattgcga tagcttcggc aattgcggaa cgatagtaga tgtctactgt ccgataagca
    41461 gagaaaatat agagacaact aaataaaaat gccgcttttt tttgttttaa aaaccgctcg
    41521 ccataatgat aagctacgag tgttgtacac aagttcaata accaaacgta aataatatag
    41581 cttacaatca aattttttgt catccaatat aacattacag ctggaaaaaa agtcagaaag
    41641 ggataaaaat aatttacacc atatccagta taattgaatg tctcataatt aatcggactg
    41701 gtaaaaatag tcgataaccc cttgattcga gataagtgaa aagaaagatc tccagtggta
    41761 taaatactct gtgtattttt gaaaaagatg aaagaatata tactactaac tataattaat
    41821 ccactcaata ttactacttt tttatttatt ttcattcttc ttgtttatct cctttttagt
    41881 cacatatcga gaatctttca acttattagg tgcaaatcgt ttaaaacttg aatacaccac
    41941 taaacaataa gtcagaatac tgatccataa agccatgaga gcagttggag tatattttgt
    42001 tgtaatttct atcttatttt ctccagcatt tacatccaca ttcactaagc catgtttttt
    42061 ataagagtca tttacgactt taccgtttac ttttacctgt gtacctaaat aagcatatac
    42121 tggtactgtc actttttgct ttttgttgct tgccactgtg taagtaatgt tattatctgc
    42181 agctttatac ttcccttttt cccacgattc tcctactttt acttcatgtt tttctaagtg
    42241 aggcctttct tttatcgctt ctgttggggc gtaatcgtat aatcctccca tatctgatgt
    42301 tgtataacgt gggagtgttt cttcattgat aacaatatga ccgtgttttg attggataac
    42361 ttttgcgtaa ttttcttttg ctgctataga cgtgacgagt agaaacacca cagtagcgcc
    42421 aatgattaac catgaatatt ttttattcat agtctttttc ataaaaacaa gcgttatgat
    42481 cattgaagaa aaaaagattt ggatacctaa gatacgccaa ggaaactgga gcaagcttat
    42541 cggtgttttt tgaaacaaaa accatggaaa taaattagtt acgcataccc atgtagaaac
    42601 tacgccaaca tagatgattt tttctttttt ctccatttct ttcagtatga ataaaggaaa
    42661 aatcaacgca accaatccta ataaccccat agtgtatgtt gtaatatcgt tatttaacgc
    42721 ttgaatgata ctatctgaaa tagaaagtgc ttctagctgc aaatctgttt tgaagggact
    42781 gtgtatttct tgataattca tctgctgcaa cataggatac caaaaatagc tagttaatgc
    42841 acaagtcaaa aaaacagcct ttactaaatt cacgaccaca aattttatat cgttttttat
    42901 ttgcttgctt aaaaacaaac gaactataat catacaagca atcaatgcac tacacaatag
    42961 tgtagttaag acatgagtat acacaagtag acttatactg acagataaag cgatgacttt
    43021 tttttctcta ttgaatatca atccataagc ataatataaa acagctggga taatcgtaag
    43081 agcgattgct tctgcaattg cggatcgata atagatatca actgttcgat aagttgcaaa
    43141 agtatataaa caactaaata aaaaagcagc atctatcttt ttaaaaaact tttctccata
    43201 ataaaaact
//
LOCUS       DACDCJ010000015        43047 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDCJ010000015 DACDCJ010000000
VERSION     DACDCJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.015"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IV038_000813"
     CDS             212..604
                     /locus_tag="IV038_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8341052.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IV038_000814"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IV038_000814"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP8341053.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="IV038_000815"
     CDS             3164..4774
                     /locus_tag="IV038_000815"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP8341054.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="IV038_000816"
     CDS             5066..5935
                     /locus_tag="IV038_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP8341055.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="IV038_000817"
     CDS             6100..7371
                     /locus_tag="IV038_000817"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP8341056.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /locus_tag="IV038_000818"
     CDS             7368..8663
                     /locus_tag="IV038_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP8341057.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="IV038_000819"
     CDS             8762..9025
                     /locus_tag="IV038_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP8341058.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="IV038_000820"
     CDS             9470..9919
                     /locus_tag="IV038_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8341059.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="IV038_000821"
     CDS             9976..11394
                     /locus_tag="IV038_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8341060.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="IV038_000822"
     CDS             11394..12254
                     /locus_tag="IV038_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP8341061.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="IV038_000823"
     CDS             12428..13201
                     /locus_tag="IV038_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8341062.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="IV038_000824"
     CDS             13348..13713
                     /locus_tag="IV038_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP8341063.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="IV038_000825"
     CDS             complement(13861..15069)
                     /locus_tag="IV038_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8341064.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="IV038_000826"
     CDS             complement(15088..15366)
                     /locus_tag="IV038_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341065.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15559..16215
                     /locus_tag="IV038_000827"
     CDS             15559..16215
                     /locus_tag="IV038_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8341066.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16657..17454)
                     /locus_tag="IV038_000828"
     CDS             complement(16657..17454)
                     /locus_tag="IV038_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP8341067.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="IV038_000829"
     CDS             complement(17451..18635)
                     /locus_tag="IV038_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP8341068.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="IV038_000830"
     CDS             complement(18660..19907)
                     /locus_tag="IV038_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP8341069.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="IV038_000831"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="IV038_000831"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP8341070.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="IV038_000832"
     CDS             21887..22201
                     /locus_tag="IV038_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP8341071.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="IV038_000833"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="IV038_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP8341072.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="IV038_000834"
     CDS             22886..23530
                     /locus_tag="IV038_000834"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP8341073.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="IV038_000835"
     CDS             23545..23874
                     /locus_tag="IV038_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341074.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="IV038_000836"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="IV038_000836"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP8341075.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="IV038_000837"
     CDS             24862..25686
                     /locus_tag="IV038_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP8341076.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="IV038_000838"
     CDS             25679..26026
                     /locus_tag="IV038_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP8341077.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="IV038_000839"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="IV038_000839"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP8341078.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="IV038_000840"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="IV038_000840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP8341079.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="IV038_000841"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="IV038_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP8341080.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="IV038_000842"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="IV038_000842"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP8341081.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31683..32450
                     /locus_tag="IV038_000843"
     CDS             31683..32450
                     /locus_tag="IV038_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341082.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="IV038_000844"
     CDS             32440..34482
                     /locus_tag="IV038_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341083.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="IV038_000845"
     CDS             34763..34912
                     /locus_tag="IV038_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP8341084.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="IV038_000846"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="IV038_000846"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP8341085.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="IV038_000847"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="IV038_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP8341086.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="IV038_000848"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="IV038_000848"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP8341087.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="IV038_000849"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="IV038_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP8341088.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="IV038_000850"
     CDS             39329..40411
                     /locus_tag="IV038_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP8341089.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40411..41214
                     /locus_tag="IV038_000851"
     CDS             40411..41214
                     /locus_tag="IV038_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8341090.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41284..42024
                     /locus_tag="IV038_000852"
     CDS             41284..42024
                     /locus_tag="IV038_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8341091.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42035..43039
                     /locus_tag="IV038_000853"
     CDS             42035..43039
                     /locus_tag="IV038_000853"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP8341092.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13840 a         7520 c         9002 g        12685 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aatttcttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgagtgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
    40441 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
    40501 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
    40561 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
    40621 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
    40681 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
    40741 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
    40801 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
    40861 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
    40921 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
    40981 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
    41041 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
    41101 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
    41161 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
    41221 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
    41281 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
    41341 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
    41401 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
    41461 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
    41521 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
    41581 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
    41641 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
    41701 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
    41761 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
    41821 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
    41881 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
    41941 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
    42001 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
    42061 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
    42121 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
    42181 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
    42241 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
    42301 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
    42361 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
    42421 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
    42481 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
    42541 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
    42601 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
    42661 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
    42721 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
    42781 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
    42841 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
    42901 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
    42961 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
    43021 gcttacctta ctttattgaa aataaaa
//
LOCUS       DACDCJ010000016        42688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDCJ010000016 DACDCJ010000000
VERSION     DACDCJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.016"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            557..1813
                     /locus_tag="IV038_000854"
     CDS             557..1813
                     /locus_tag="IV038_000854"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP8341093.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            2104..3981
                     /gene="tdc"
                     /locus_tag="IV038_000855"
     CDS             2104..3981
                     /gene="tdc"
                     /locus_tag="IV038_000855"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP8341094.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            4224..5645
                     /locus_tag="IV038_000856"
     CDS             4224..5645
                     /locus_tag="IV038_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8341095.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            5754..7121
                     /gene="nhaC"
                     /locus_tag="IV038_000857"
     CDS             5754..7121
                     /gene="nhaC"
                     /locus_tag="IV038_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8341096.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(7402..8421)
                     /locus_tag="IV038_000858"
     CDS             complement(7402..8421)
                     /locus_tag="IV038_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8341097.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8443..9450)
                     /locus_tag="IV038_000859"
     CDS             complement(8443..9450)
                     /locus_tag="IV038_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8341098.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(9443..10108)
                     /gene="pgmB"
                     /locus_tag="IV038_000860"
     CDS             complement(9443..10108)
                     /gene="pgmB"
                     /locus_tag="IV038_000860"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8341099.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(10101..12395)
                     /locus_tag="IV038_000861"
     CDS             complement(10101..12395)
                     /locus_tag="IV038_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8341100.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            12655..14820
                     /locus_tag="IV038_000862"
     CDS             12655..14820
                     /locus_tag="IV038_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8341101.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            15124..15957
                     /locus_tag="IV038_000863"
     CDS             15124..15957
                     /locus_tag="IV038_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP8341102.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(16059..17759)
                     /locus_tag="IV038_000864"
     CDS             complement(16059..17759)
                     /locus_tag="IV038_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341103.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            18266..19426
                     /locus_tag="IV038_000865"
     CDS             18266..19426
                     /locus_tag="IV038_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8341104.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            19442..19885
                     /locus_tag="IV038_000866"
     CDS             19442..19885
                     /locus_tag="IV038_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8341105.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(20368..20898)
                     /locus_tag="IV038_000867"
     CDS             complement(20368..20898)
                     /locus_tag="IV038_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP8341106.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            21003..21554
                     /locus_tag="IV038_000868"
     CDS             21003..21554
                     /locus_tag="IV038_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8341107.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(21707..22180)
                     /locus_tag="IV038_000869"
     CDS             complement(21707..22180)
                     /locus_tag="IV038_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8341108.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            22318..23790
                     /locus_tag="IV038_000870"
     CDS             22318..23790
                     /locus_tag="IV038_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8341109.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            23787..24497
                     /locus_tag="IV038_000871"
     CDS             23787..24497
                     /locus_tag="IV038_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP8341110.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            24849..25133
                     /locus_tag="IV038_000872"
     CDS             24849..25133
                     /locus_tag="IV038_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP8341111.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(25414..25833)
                     /locus_tag="IV038_000873"
     CDS             complement(25414..25833)
                     /locus_tag="IV038_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP8341112.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(25835..26419)
                     /locus_tag="IV038_000874"
     CDS             complement(25835..26419)
                     /locus_tag="IV038_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP8341113.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(26466..27941)
                     /locus_tag="IV038_000875"
     CDS             complement(26466..27941)
                     /locus_tag="IV038_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP8341114.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            28122..28433
                     /locus_tag="IV038_000876"
     CDS             28122..28433
                     /locus_tag="IV038_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341115.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(28548..30884)
                     /locus_tag="IV038_000877"
     CDS             complement(28548..30884)
                     /locus_tag="IV038_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8341116.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            31026..31694
                     /locus_tag="IV038_000878"
     CDS             31026..31694
                     /locus_tag="IV038_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8341117.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            31697..32740
                     /locus_tag="IV038_000879"
     CDS             31697..32740
                     /locus_tag="IV038_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8341118.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(32977..33858)
                     /locus_tag="IV038_000880"
     CDS             complement(32977..33858)
                     /locus_tag="IV038_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8341119.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            34017..34745
                     /locus_tag="IV038_000881"
     CDS             34017..34745
                     /locus_tag="IV038_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP8341120.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            34901..35218
                     /locus_tag="IV038_000882"
     CDS             34901..35218
                     /locus_tag="IV038_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8341121.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            35211..35819
                     /locus_tag="IV038_000883"
     CDS             35211..35819
                     /locus_tag="IV038_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP8341122.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            35874..36665
                     /locus_tag="IV038_000884"
     CDS             35874..36665
                     /locus_tag="IV038_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HAP8341123.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GEFYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            36803..37291
                     /locus_tag="IV038_000885"
     CDS             36803..37291
                     /locus_tag="IV038_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP8341124.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            37342..38214
                     /locus_tag="IV038_000886"
     CDS             37342..38214
                     /locus_tag="IV038_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP8341125.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            38390..38752
                     /locus_tag="IV038_000887"
     CDS             38390..38752
                     /locus_tag="IV038_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8341126.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            38761..39513
                     /locus_tag="IV038_000888"
     CDS             38761..39513
                     /locus_tag="IV038_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341127.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            39527..41329
                     /locus_tag="IV038_000889"
     CDS             39527..41329
                     /locus_tag="IV038_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341128.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            41606..42646
                     /locus_tag="IV038_000890"
     CDS             41606..42646
                     /locus_tag="IV038_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8341129.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        13339 a         7561 c         8833 g        12955 t
ORIGIN      
        1 tttttttact tgtcccattc cttattgtaa cgaagaaaga ataagaggca aagtaaagtg
       61 acttctcgat aaattgcaga aaaaatacag aaaaagttat ttcttttaac aataattgaa
      121 aataaaatgt tcggaattag agcggattca aaaaagaaaa aatgaggaat ttaaaaaaaa
      181 tatccttttt ctgttgacaa agaaaaagtt gcgctctatg atagacacat attaataata
      241 acaagacgaa gatcgaaacg aagtagttgt taaagccaat gtttcagaaa gctggtggtc
      301 gctgcaaacc agtacatgct ttaaagcgaa ttacatttca ggagtctttg gcagtgattg
      361 ccacgcgaca gcgttaaatg ttcgagaggt atgttggata tctagacata caaattgggt
      421 ggtaccgcga gatttcgtcc cttggcttat atgccaaggg acttttttta tttcctatcc
      481 tatcctgaag aaataattaa ttacacatgc tagcaggttc ctgctttcat aaaaaaacta
      541 cacggaggtc agtaaaatga atattattga tgaattaact tggcgcgacg ccattaatca
      601 gcaaacaaac gaagaaagac ttcgcgagct cgttgaagaa aagagcattt cactttattg
      661 cggcgttgat ccaactggtg attcgatgca tatcggacat ttgattccat ttatgatgat
      721 gaaaagattc caattggcag gccatcatcc atatattctg attggcggcg gaacaggaac
      781 gatcggcgac ccaagcggcc gcaaaacaga acgtgtgttg caaacgatgg aacaagtgca
      841 acataacgtg gacgcacttt ccaatcaaat gagaaaattg tttggtaaag atgcaaatat
      901 cacatttgtg aacaactacg actggttgtc aaaaatctct ttacttgaat ttttgagaga
      961 ctacggtaaa aacttcaaca tcaacacgat gttagcaaaa gatatcgttg ccagccgttt
     1021 ggaagtagga atctcattta cagagtttac ttaccaaatc ctgcaatcta tcgacttttt
     1081 gcatctgcat aaaacatacg atgtgcaatt gcaaatcggt ggagcagacc aatggggaaa
     1141 tatcacagct ggattagacc taatccgaaa attagaagga ccagaagcag aagcgttcgg
     1201 tttaacgatc ccattgatgc tgaaggctga cggaacgaaa ttcggaaaaa ctgcaggcgg
     1261 tgccgtttgg cttgatccga agaaaacttc accatttgag ttctaccaat tctggttgaa
     1321 tcaagatgat cgcgatgtag tgaaatactt gaaattcttt actttcttgt ctcaagaaga
     1381 aatcgaagac ttagcgaaaa aagtagagac agaacctgag aaacgtgaag cacaacgccg
     1441 tttggcagaa gaagttacaa gatttgtgca tagtgaagaa gacttgaaag aagcgcaaaa
     1501 aataacgcaa gcattgttct ctggaaatat taaagaacta aatgcggaag aaatcgcgca
     1561 aggattcggt aaaatgccga acgttgaaat ttcaagcaca cctgaaaata tcgtggaact
     1621 gcttgtttcc acaaaaattg aaccatctaa acgtcaagca cgtgaagatg tttcaaacgg
     1681 agctataagt attaacggtg accgcgttac cgatttaaat tttgtcataa atccatccga
     1741 tgaattcgat ggtaagtttg tggttattcg aaaaggtaag aaaaattact ttttggccaa
     1801 agtaattgat tagaatttag taagttttta ccttgaaaaa gggaaatact attacaacat
     1861 tccaattttg aaacccatcc taaaatagaa cgctggttgt cgttaataca atctttgcga
     1921 gggagtttac atttgtaaat tccctcgttt tttttaatta tttcaaaagt gtgaacaatt
     1981 cggataattg atacatatta aaatatgatt aaaaatcagg ccaaaactta gtttacagaa
     2041 tagaagaaca ggtgtaatgt agggctcgac aaaatttaaa tattttttta ggagggtttc
     2101 actatgagtg aatcattgtc gaaagatcta aacctaaatg ccctattcat tggggacaaa
     2161 gctgagaacg gccaaatcta taaagctttg ttgaatgaat tggtggatga acatttaggc
     2221 tggcgtcaaa actacatgcc tcaagacatg cctattatta cgccagaaga aaaaagcagt
     2281 gctagttttg aacacactgt gaataaaaca aaggatgttc tatcagaaat ttcagcgcgg
     2341 atgcgtaccc attccgttcc ttggcataat gccggacgtt actggggtca tatgaactct
     2401 gaaacattga tgccatcttt actagcgtac aattttgcta tgctatggaa tggtaataac
     2461 gttgcctatg aatcttctcc tgcaacaagc caaatggaag aagaagtagg aatggaattt
     2521 gcaaaattaa tgagctataa agatggctgg ggccacatcg ttgctgatgg ttctttagca
     2581 aacttagaag gactttggta tgcccgcaac atcaaatcat tgccgcttgc aatgaaagaa
     2641 gtgacacctg aactagttgc tggaaaaagc gattgggaat taatgaactt gtcaacagaa
     2701 gaaatcatga atctgttaga cagcgtccca gaaaaaatcg acgaaatcaa agctcactca
     2761 gcacgtagcg gaaaacattt agaaaaatta ggaaaatggt tggttccaca aactaaacac
     2821 tattcatggc taaaagctgc cgacatcatc ggtatcggtt tagaccaagt gattccagtg
     2881 ccagttgacc acaactatcg catggacatc aacgaacttg aaaaaatcgt tcgtggtcta
     2941 gcagctgaaa aaacaccaat cctaggtgta gtcggtgttg taggatcaac agaagaagga
     3001 gcaatcgacg gtatcgataa aatcgttgca ttgcgtcgcg tattagaaaa agacggtatt
     3061 tacttctact tacacgtaga tgctgcttat ggcggatacg gccgtgcgat tttcttagac
     3121 gaagacaaca acttcattcc atttgaagat ttaaaagatg ttcattacaa atacaatgtc
     3181 tttacagaaa acaaagatta tatcttagaa gaagtacaca gtgcatataa agcaatcgaa
     3241 gaagcagaat ctgtaacgat tgacccacat aaaatgggct atgttccata ttctgccggt
     3301 ggtatcgtca tcaaagatat ccgcatgaga gacgttatct cttactttgc gacatatgta
     3361 tttgaaaaag gcgcagatat cccagcatta cttggggctt atatcttgga aggttcaaaa
     3421 gcaggggcaa cagcagccag cgtttgggca gcacaccatg tcttaccttt gaatgtcaca
     3481 ggatatggta aattaatggg tgcatcaatc gaaggtgcac atcgtttcta caacttcttg
     3541 aatgacttgt catttaaagt aggagacaaa gaaatcgaag ttcatccatt aacttatcca
     3601 gatttcaata tggtagacta tgtattcaaa gaaaaaggca acgatgactt agttgcaatg
     3661 aataaattga accatgacgt atatgattat tcttcatacg tgaaaggaag catttacggt
     3721 aatgagttct taacttcaca tactgacttt gctatcccag actatggcaa cagcccatta
     3781 caatttgtga accaactagg attctcagat gaagaatgga accgtgccgg aaaagtaact
     3841 gtattgcgtg ccagcgtaat gacaccatac atgaacaaag aagaacactt tgaagaatat
     3901 gctgaaaaaa tcaaagcagc tcttcaagaa aaattggaaa aaatctacgc agaccaatta
     3961 ttggcaagcg aagcaaaata agcaaggtaa tcatgttaga aaacagcttg aatactaact
     4021 gtttctgacg catgtttgga tctgaagagc aaggggagac ctaagctctt cgatcccttt
     4081 gttttgaggt tgtctttgaa gaaacccttc aagcaataaa ctcaaagaat cccctcaaat
     4141 attcgctgaa ttgtttcgtt tattgctgaa actacttcaa agacaccgtt attttatcca
     4201 aaaaatcaag gagagtgtca acggtgagta aatctgaagt aagtaaaatg ggtcttggga
     4261 cctttatcgg tctgacaatg gcactgtgtg caacggttcg gagtattcct acgttagccg
     4321 cagtcggttg gacattgatt ttttattcaa tttttgcagt tttgtttttt gccggtccga
     4381 tatcaatggt ttccggtgag ctttccacga tgcttcctca agaaggcggt cctcagttat
     4441 gggtaaaaac agcactgggc agcaaatggg gatttgtcgt tgcatggcta ttatgggtgc
     4501 aaatgttccc aggtatggta atggtcgctt ctactttagg accattgtta ggaaacacat
     4561 ttggtaatgt agaacttggc aataatcact ggttcgtttt aggttgtatt ttagttatct
     4621 actggatcat cacgatcttg aacttgaaat tcgatatggc aaaagtcggc gggaacatcg
     4681 gtgtatggct aggggtatat atcccagtag tcatcatgtt cgttttaggt gttttggcag
     4741 ccttcaaagt cggactagtc tcaaacggct atctaggtga cttttcatgg tcaaaagctt
     4801 tccctgactt ggaacatatt gattcattga aatacttagc tggtatcaca tttatttttg
     4861 ttgggatcga aatgagttct gtttacatgc ctcgtttgaa agacgcaaca aaaaattata
     4921 caaaaggcgt atttatcgct ttgatcggat tagttttatt gaatgtcatc aatgcgatgc
     4981 ttgtagcaaa tgtcgtacca gatggaaaaa tggaattagc aaatatcacg cagcctatct
     5041 tgattgactg tcaaatccta ggcttaccag aagtgatcgg aaatatcttc agcttcatgg
     5101 tatttattgg tgtgctttta caattatcag cttgggttac tggaccatca aaaacaatca
     5161 tccaagtagc tcgtgaagga ttccttccac caaaattcgg attccataaa gaaaacaaat
     5221 acggcgtatc tagaaatgtc gttctaacac aatcaatcgt tatttcatta tttgctttgc
     5281 tttatggtgt aatggatgac gtaagtgctg tattcttgac acttacaaat gctacaactg
     5341 ttatttattg tatcgtgtac attttgattg ctgtttcatt gcttaaaatg cgtaagaaac
     5401 atccagaatt cgaacgtcct taccgaattg gtaaaaacgg aaacggatta gcttgggtag
     5461 tcagctgcat gctgatcttc tctatcatcg ttgtcgtatt tgctacatta ggtacagcga
     5521 cattgagtga tgcattatta gtagcagcta tcacagtggt catgttcgtt attccattga
     5581 tcatcaatca cttcaagaaa gattcttggg ggattgaggt agaaaaaagt ttagaagaaa
     5641 aataaaaaaa taagtctttc agccgacgtt ttgtcgctga tttagtagga ggtcctgcca
     5701 agtgaatagt tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata
     5761 aaaatatttc tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag
     5821 tactgatcat cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt
     5881 tgttgtttta tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca
     5941 aaggaatcac tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg
     6001 cttcatggat tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt
     6061 ctgtgcgttt ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg
     6121 gcagttcatt tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac
     6181 ttggattcaa tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata
     6241 actgctcgcc tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat
     6301 tcgaacatat cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt
     6361 tttatacgat attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga
     6421 tccaaaacat gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt
     6481 tatttggggg ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag
     6541 ctgctctcgt tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca
     6601 tcttgatgaa tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc
     6661 gaggcgggat catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag
     6721 gtgggttact gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga
     6781 tggaccgacc ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga
     6841 tcgttggaga acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg
     6901 atgacgttgg cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg
     6961 tcaattctct cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta
     7021 gcgcgttgca ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat
     7081 taggcggttt tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct
     7141 gggacagaag cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat
     7201 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
     7261 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac
     7321 aatgtgttag aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag
     7381 cgtatttagt gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt
     7441 gatcaagcga tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc
     7501 gcgcagcttt tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag
     7561 gtaagggtgt gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc
     7621 tggaatgaca tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc
     7681 aatgaccacc aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc
     7741 ctccggatgc tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt
     7801 ttgataaccg aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag
     7861 gatattctta tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt
     7921 atcattatct acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa
     7981 agggatgtgg ttttggtata aataatcaat gacagtatct tgactatctg aatacactag
     8041 aataaaacca tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc
     8101 aaaattttta gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat
     8161 tgcttccatg atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg
     8221 taagattacg ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg
     8281 aacataacct agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact
     8341 aggatgatcg ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac
     8401 atcttttacg gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg
     8461 ataaatcgtc tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg
     8521 gaaatgaacg atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat
     8581 ctgttttcca ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact
     8641 tttattagaa gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga
     8701 agttttggaa agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca
     8761 tgcaaggatc tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt
     8821 tgtccaattt ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac
     8881 cgttaactga tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg
     8941 gttggtcgga ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc
     9001 gttttttgtt aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc
     9061 atcttttaag taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca
     9121 aaattggaaa gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc
     9181 caatctatgc tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc
     9241 tccaaaaaag cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt
     9301 ttctgatttt ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag
     9361 aagaacgact tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac
     9421 tggtacgacg gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga
     9481 tcgtaagaaa agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc
     9541 ccacgccaat tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt
     9601 cgatcccgat acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat
     9661 ctggcgcagg ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg
     9721 taagacccat tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct
     9781 tgattttttc ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg
     9841 gttcgatcgt ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa
     9901 attcttcctt tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt
     9961 cttctcggct gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt
    10021 cctgaccaag ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt
    10081 ctaaatcaaa caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga
    10141 cgatccgcta aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat
    10201 aactcgaaag taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg
    10261 atatggaaag aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca
    10321 tgggcagttt tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa
    10381 gtgatgtgca agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt
    10441 cgctggtaca tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg
    10501 atacttggcg aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt
    10561 tgcttttctt cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc
    10621 tgcttgatga agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga
    10681 tcgcttgtgg gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg
    10741 acaaagattc ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat
    10801 ttggctagtt cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt
    10861 tctcgcgtgt aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata
    10921 ttgttctcat attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt
    10981 gaaaaatgga cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca
    11041 tttttcaaat attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc
    11101 gcgccatttc ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca
    11161 gtgaaggtga ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt
    11221 gcttgcggca gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct
    11281 aaagcaagat aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat
    11341 ttttctcctg tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta
    11401 gagaatagtt ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg
    11461 accacatcgg caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg
    11521 atataattgt ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct
    11581 ggaatatctc tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga
    11641 gataattctg cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg
    11701 actgattttc cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca
    11761 aagttgtttg gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca
    11821 atctcattcc aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat
    11881 tttgatacga gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa
    11941 gctgattctt ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta
    12001 gaagtagtga ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa
    12061 aagtcttctg gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc
    12121 gcgataaagt tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac
    12181 cagccgacac gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg
    12241 tcccctgagt aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt
    12301 gttaaagatt cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat
    12361 ggatcaatag caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt
    12421 atacgttcat aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat
    12481 agaaaaaagc ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt
    12541 ataggtttta tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa
    12601 aacgcacgct ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa
    12661 aagatattta gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca
    12721 gttatgccgg cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct
    12781 gattggacgc cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt
    12841 ggtaatcttc atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca
    12901 ggcggagcat ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc
    12961 tttggtgtca caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt
    13021 acgaagatca tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg
    13081 ggagtattcg tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat
    13141 aattatagaa aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt
    13201 gttgttatct tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta
    13261 caggctggaa tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt
    13321 ctagcaccgt ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat
    13381 atgctgacga ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc
    13441 gcacaggcgg ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat
    13501 ttagtgaatc ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg
    13561 acgccggctc gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg
    13621 gcttttgcga tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat
    13681 ttttcagcag cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt
    13741 atgtttgctg ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg
    13801 atggcagata tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg
    13861 acaccgttag ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg
    13921 atcgcttttg gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt
    13981 gctacaccgg gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac
    14041 agcggaacag gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa
    14101 gaaaatatca aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga
    14161 gaaaaagtag ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa
    14221 gacaatggag tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa
    14281 gatctgctgc aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga
    14341 caagcaggta gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga
    14401 gaagtgattc ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac
    14461 ggctttgctg ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca
    14521 agtattttcc ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg
    14581 atccatatgg gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa
    14641 gaaggccaag aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa
    14701 aacgaagaaa aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta
    14761 gtgatcaagc aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa
    14821 ggagaggttg tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc
    14881 tcctcctatt tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt
    14941 agtggagagg tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa
    15001 tagctcctgg tccattgagc ttattactga ggagttagca ggagaacact atttgctgga
    15061 gaggttgtga caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa
    15121 ccaatgaaat tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa
    15181 ttgcaagaat tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa
    15241 atcaaccaat tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg
    15301 gtaaagaatc aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta
    15361 aaggaacacg gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa
    15421 tatgaagaag gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc
    15481 tcccaatttc aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag
    15541 atagatgatc aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa
    15601 agtggctttt attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca
    15661 ctttttttcc taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga
    15721 gaaagttgcc ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct
    15781 actgtggaaa agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt
    15841 attttcgtcc ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat
    15901 agtccaatca tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa
    15961 atataacata taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt
    16021 tggagagttt tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat
    16081 ctatgataaa taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg
    16141 aaatagaaat agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc
    16201 ttcacgattg gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc
    16261 tgtagcacag tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt
    16321 ttccacttga ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct
    16381 agtatttctt cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga
    16441 tagtcaggtg tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat
    16501 gttttccttg cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca
    16561 ttttcccact tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga
    16621 atgccaaata tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg
    16681 gcaaggtaaa gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc
    16741 aaactaataa aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa
    16801 agaaaaaaga aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt
    16861 tgaaagaaaa taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa
    16921 tggagccaat aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag
    16981 ggaggaagaa tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc
    17041 agaacaaaat aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc
    17101 tggttaaaaa agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac
    17161 agtaaatgga tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt
    17221 ttatgaagga cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag
    17281 ctagtgaatc cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg
    17341 gctactggta cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa
    17401 aatcctgaaa taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct
    17461 gcaagcaaca cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg
    17521 atcctgccac tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta
    17581 atttgttgcg cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt
    17641 agtttatgtg tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa
    17701 aatgggccat attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata
    17761 tatcctatgc gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta
    17821 caagaaaaac ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc
    17881 tccttgtttt gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct
    17941 ctcggtaaac ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata
    18001 tgagaagcta ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac
    18061 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa
    18121 aatatgagta gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac
    18181 aacctcatcg gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca
    18241 aaaaaacttg caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact
    18301 tttgtttcgt ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc
    18361 attttatgtg gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa
    18421 acggaccgca gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt
    18481 aactgaccaa ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag
    18541 tacaacatcc agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca
    18601 attaaatatt tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt
    18661 cttgcaagga acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca
    18721 cagcggttta cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac
    18781 gtttattttg actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt
    18841 actgccagat gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct
    18901 tacatgtacg ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc
    18961 ttataacgaa tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca
    19021 atggtttatt cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg
    19081 actaatctat cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga
    19141 ttcgtcaggg aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact
    19201 tactagaaat ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga
    19261 tcaattgcct ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc
    19321 ttttggtatt agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt
    19381 aaaagagctt caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa
    19441 aatgatccgc acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg
    19501 gctgcatgct aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt
    19561 agaatctgtt aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga
    19621 aattattggt tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga
    19681 ataccggcaa caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac
    19741 actgactttg acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg
    19801 ttttacaatt caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa
    19861 atggaatcaa aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat
    19921 tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt
    19981 tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
    20041 ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga
    20101 tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag
    20161 cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct
    20221 gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa
    20281 aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc
    20341 tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta
    20401 ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc
    20461 attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt
    20521 tggataagtt tcatattttt cgcgagctgc ttccatttct gcgatatgtt cgttttggaa
    20581 agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag
    20641 tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt
    20701 gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg
    20761 gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca
    20821 ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa
    20881 ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac
    20941 atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac
    21001 tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga
    21061 tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa
    21121 aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc
    21181 aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga
    21241 aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg
    21301 aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta
    21361 ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa
    21421 cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc
    21481 atgcgatcaa tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta
    21541 accgaaaaaa ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa
    21601 acgcagatca ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt
    21661 agcatctact tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta
    21721 acaactgttc gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg
    21781 tctcgttgtc atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta
    21841 aaatcgtata gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac
    21901 ggacatggtg cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa
    21961 atccgtcatc taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt
    22021 ttctaggaca attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc
    22081 ttaattgacg tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt
    22141 gatcattttc caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc
    22201 tcttgcttcc cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg
    22261 aatttaggac taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg
    22321 agtaacaacc aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt
    22381 gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt
    22441 cctacattga tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt
    22501 ttcttgctag tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt
    22561 tcaagaaatt tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt
    22621 acagcaccta attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga
    22681 gtatcgatgc cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga
    22741 tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta
    22801 tctggttata tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc
    22861 gttgttcttg tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat
    22921 cctagcattg atattttatc cgctatttta tcaacgattg gattcggtag tttactttac
    22981 ggattttcta gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt
    23041 gttggtgtga ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc
    23101 ttggaacttc gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta
    23161 acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt
    23221 ggagaatcag cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct
    23281 atgatgcctg tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca
    23341 gggatgttca ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca
    23401 gttttatata tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg
    23461 ccggtaacga cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg
    23521 gtaaataata cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg
    23581 ttgacgaatg tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta
    23641 gcttataaag atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta
    23701 gcgatcatct ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct
    23761 gttctaatga aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg
    23821 cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt
    23881 tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg
    23941 gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag
    24001 gagcagatat gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc
    24061 gaacaaatga aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag
    24121 tagaaaagac agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa
    24181 acgacatgta taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag
    24241 tgaatacatt caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt
    24301 tagctgaatt agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc
    24361 aagacggtat ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac
    24421 aacgtacgaa agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag
    24481 aagcaaaagg cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc
    24541 atcaagcagt aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct
    24601 tagtgccgta gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt
    24661 taactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt
    24721 gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca
    24781 agacttttgt cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga
    24841 gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca
    24901 agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag
    24961 ttaaagtcca agcaactttt gacaatgtgc catatgaagg cagtatcgtg aatatgggcg
    25021 tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa
    25081 acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt
    25141 tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt
    25201 agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact
    25261 cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg
    25321 gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact
    25381 caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata
    25441 ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc
    25501 taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg
    25561 gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt
    25621 ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg
    25681 catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt
    25741 taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag
    25801 tttggttact tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat
    25861 gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt
    25921 ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc
    25981 gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa
    26041 tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc
    26101 ctaaaccaac agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat
    26161 cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat
    26221 caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg
    26281 cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta
    26341 ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc
    26401 gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc
    26461 attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa
    26521 tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg
    26581 aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc
    26641 attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact
    26701 acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct
    26761 ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt
    26821 tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca
    26881 tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga
    26941 ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg
    27001 tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca
    27061 gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt
    27121 gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca
    27181 tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat
    27241 agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct
    27301 ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt
    27361 tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct
    27421 tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag
    27481 actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa
    27541 atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg
    27601 tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga
    27661 ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt
    27721 ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agttggtcct
    27781 tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat
    27841 tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt
    27901 tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt
    27961 gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa
    28021 agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta
    28081 tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt
    28141 catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa
    28201 acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat
    28261 ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga
    28321 ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt
    28381 atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa
    28441 ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac
    28501 tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc
    28561 aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc
    28621 gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca
    28681 ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca
    28741 ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg
    28801 cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt
    28861 tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc
    28921 cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc
    28981 atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa
    29041 tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata
    29101 cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca
    29161 actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat
    29221 tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat
    29281 aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca
    29341 gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt
    29401 tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa
    29461 tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta
    29521 ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca
    29581 agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa
    29641 cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta
    29701 tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga
    29761 ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt
    29821 acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg
    29881 acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca
    29941 tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt
    30001 tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg
    30061 attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt
    30121 cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag
    30181 ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca
    30241 tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc
    30301 atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt
    30361 tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt
    30421 gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc
    30481 atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct
    30541 ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca
    30601 atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca
    30661 ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga atcataatta
    30721 tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc
    30781 gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt
    30841 tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg
    30901 tattgaaata ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata
    30961 aatgttaact gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga
    31021 gatcaatgaa tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt
    31081 ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga
    31141 aaaaactaga agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg
    31201 atggctatga cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga
    31261 ttacggcaaa ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt
    31321 atatgacaaa acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac
    31381 gttcaaagat caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg
    31441 cttatacagt gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc
    31501 tgtataaatt actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata
    31561 tttggggatt agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa
    31621 ggacccgttt tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata
    31681 aggcggttat ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag
    31741 tattgtcttc atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt
    31801 gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt
    31861 cgtctttgcg gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg
    31921 tatcctgcat ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt
    31981 ttcgattcga atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt
    32041 caacgttatg gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag
    32101 tgtatcccat gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca
    32161 agccccaaat ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat
    32221 cacgcagctt tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg
    32281 tatccaactg gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt
    32341 ttttcagcct aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa
    32401 ttacacaggc aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat
    32461 aaagtacaat caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat
    32521 tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt
    32581 tgaaaaattt tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc
    32641 actggttaaa aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg
    32701 agtaggaacg acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta
    32761 aaaattctat tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt
    32821 cttacggttc ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga
    32881 ggtgtcattg aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct
    32941 ttttttgggt ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata
    33001 ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata
    33061 actctctgtt gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac
    33121 tgttttttcc ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc
    33181 gattcttgct cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag
    33241 tacaggttga tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc
    33301 gcttagatcc aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa
    33361 atcgtctgtt tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc
    33421 aaagtggatc gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac
    33481 ttctttcaac tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa
    33541 acctttataa ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt
    33601 cgtataggtt tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata
    33661 tgggagcaat aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa
    33721 tttgacgtct aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt
    33781 gaattgtcga tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc
    33841 gaatttatgg atatccatca tattcctcct acttattcca ataaagaata ctttaacaca
    33901 aaaaatgaat tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata
    33961 attacagaaa tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc
    34021 caactggagt tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag
    34081 taggaaataa attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt
    34141 cgatggggat gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg
    34201 ctttagtgat aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg
    34261 gggctgcgct gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga
    34321 cccacgatga atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta
    34381 cggggatcta tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta
    34441 aatctgtcct tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt
    34501 ctgtgatagc aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta
    34561 tttatccatt gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga
    34621 tggtcgctac cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac
    34681 ctgcaatgat cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt
    34741 tataataaaa atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt
    34801 cagttttttt gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga
    34861 ttgaaataaa atattacact tgagaataga aggtgacgta atgcacatcc agattccaac
    34921 tgttcttctt gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc
    34981 cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt
    35041 gagaagattg aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa
    35101 ccgtcgatat tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga
    35161 atggattgtt tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat
    35221 atttgaatga agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga
    35281 tccagtatta tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg
    35341 aatatggtac accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag
    35401 aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa
    35461 tcattatcgg tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg
    35521 ttattggggg actattggtt gctttactag cgctcatttt tagtatctat gttttatgta
    35581 tcagtatctt aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat
    35641 cgattctgag tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat
    35701 ttatttcgcc actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg
    35761 ttaaatggat gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa
    35821 ggaggacaca taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta
    35881 ctggtggcct cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg
    35941 ataatggtcc tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca
    36001 aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga
    36061 taaaaacgac ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga
    36121 ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac
    36181 aaatcactat tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata
    36241 atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg
    36301 gctgggtatc gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct
    36361 cacttcgatt agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact
    36421 tccatctaga agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt
    36481 tatctttgca tgagatggaa aacagtggct atactatcca aacgtctggt gaattttata
    36541 ttgaaaaaga ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa
    36601 agatcactgc taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag
    36661 attgatcctg tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt
    36721 gctgtttttc atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa
    36781 agaaaacagg gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac
    36841 agaaatggaa agattacata cttatatcac tcaactaagc caaaccttct atgatccaga
    36901 caaagaaaaa gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt
    36961 gtaccatgaa gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt
    37021 accgattatc gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa
    37081 atctgttcgg gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga
    37141 ctattttaca gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc
    37201 cggagggaca caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg
    37261 tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga
    37321 taaaggagcg caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca
    37381 gggattgtcg gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg
    37441 tggcaagcga aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg
    37501 ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga
    37561 caatatttag gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa
    37621 atgactgttt cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg
    37681 gatggaaacc ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag
    37741 gcattattcg atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt
    37801 cggcatatcg ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga
    37861 ggaaacacga cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta
    37921 gcaggtgtcg aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct
    37981 agtgccatgc tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt
    38041 gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc
    38101 aatttcaccg atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca
    38161 gacaaaggaa cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact
    38221 ttttgtaaga taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt
    38281 tgttttaaag aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg
    38341 ttcgcttttt tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact
    38401 tctgatcggt ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta
    38461 cgtagttatg ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt
    38521 ttcaaaatca gactctggac aagaaacagt caactataga tatatcctgc ctggctatac
    38581 gaaagagggt aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa
    38641 aggagcatat ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt
    38701 cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa
    38761 atgaacaaaa tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa
    38821 aagacgcaag cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc
    38881 atgggcgctt ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa
    38941 ccaacagatg ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa
    39001 ctagcagatt ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa
    39061 agcttgacag cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa
    39121 gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa
    39181 agctatcctt ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg
    39241 atcacacaac caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt
    39301 gcccgggatc ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt
    39361 cttgtgacgc atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat
    39421 ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa
    39481 atactatcta ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt
    39541 atcacttact gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt
    39601 tatggcatct gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa
    39661 aagtaatacg atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg
    39721 gatcatcaca ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa
    39781 agactatgca atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt
    39841 ttctgaaaca ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg
    39901 attgacaagt atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt
    39961 tacacctttt agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt
    40021 attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg
    40081 tgcggatcag acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt
    40141 actgggaatt gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca
    40201 agtctttggt ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca
    40261 ttctgtgatc attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa
    40321 attgaacaat ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct
    40381 ctcaatggtc gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt
    40441 taaaaatgaa attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa
    40501 tcctcgggca gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta
    40561 tactcaaaaa gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc
    40621 attgatcatg ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc
    40681 tgctaaaaaa atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga
    40741 acagcaagaa aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc
    40801 acaatccact ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga
    40861 aacacttgtt gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa
    40921 ccagaaatat gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg
    40981 attcttccta ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt
    41041 gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg
    41101 cgggttgctt aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat
    41161 cttaggtgta gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc
    41221 atacaaagat atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt
    41281 actgacgaca tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc
    41341 tgcatcaagt aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg
    41401 gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca
    41461 atatttttgt cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc
    41521 gttactcacg gaataagctg ttaagaaaac ggtttccatg tatgctggaa tcagtgcaaa
    41581 tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta
    41641 tcacttgatt acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc
    41701 acgggttgtt cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt
    41761 tgatgaagca aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat
    41821 ggctggacgg attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac
    41881 agacccggac actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg
    41941 gcattataca gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc
    42001 agatatggaa aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa
    42061 cgatgataca tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc
    42121 tacaaatcat gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat
    42181 actttggttg aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga
    42241 agtagtttca gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt
    42301 ctttgccagt gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt
    42361 gaaaaaacaa tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc
    42421 gatccaagta aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga
    42481 tggaccggaa atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca
    42541 agaactgcca ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca
    42601 agtacgcgag tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaaaag
    42661 ggagtgtgac agtgtcacat cccctttt
//
LOCUS       DACDCJ010000017        41902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDCJ010000017 DACDCJ010000000
VERSION     DACDCJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.017"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            134..409
                     /locus_tag="IV038_000891"
     CDS             134..409
                     /locus_tag="IV038_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP8341130.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="IV038_000892"
     CDS             415..1182
                     /locus_tag="IV038_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP8341131.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="IV038_000893"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="IV038_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP8341132.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IV038_000894"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IV038_000894"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP8341133.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IV038_000895"
     CDS             5326..5628
                     /locus_tag="IV038_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP8341134.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IV038_000896"
     CDS             5631..6794
                     /locus_tag="IV038_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8341135.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IV038_000897"
     CDS             6820..10248
                     /locus_tag="IV038_000897"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP8341136.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IV038_000898"
     CDS             10269..11393
                     /locus_tag="IV038_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341137.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IV038_000899"
     CDS             11383..11820
                     /locus_tag="IV038_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8341138.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IV038_000900"
     CDS             11804..12142
                     /locus_tag="IV038_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP8341139.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IV038_000901"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IV038_000901"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP8341140.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IV038_000902"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IV038_000902"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP8341141.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IV038_000903"
     CDS             13299..14342
                     /locus_tag="IV038_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8341142.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IV038_000904"
     CDS             14501..15229
                     /locus_tag="IV038_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP8341143.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IV038_000905"
     CDS             15345..15590
                     /locus_tag="IV038_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8341144.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IV038_000906"
     CDS             15587..15805
                     /locus_tag="IV038_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP8341145.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IV038_000907"
     CDS             15941..16480
                     /locus_tag="IV038_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP8341146.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IV038_000908"
     CDS             16473..18746
                     /locus_tag="IV038_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP8341147.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IV038_000909"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IV038_000909"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP8341148.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IV038_000910"
     CDS             complement(19919..20173)
                     /locus_tag="IV038_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP8341149.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="IV038_000911"
     CDS             complement(20573..20842)
                     /locus_tag="IV038_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341150.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21312..21767)
                     /locus_tag="IV038_000912"
     CDS             complement(21312..21767)
                     /locus_tag="IV038_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP8341151.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="IV038_000913"
     CDS             complement(21905..23833)
                     /locus_tag="IV038_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP8341152.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24030..24863)
                     /locus_tag="IV038_000914"
     CDS             complement(24030..24863)
                     /locus_tag="IV038_000914"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP8341153.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25055..25759)
                     /locus_tag="IV038_000915"
     CDS             complement(25055..25759)
                     /locus_tag="IV038_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8341154.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGAIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25782..26408)
                     /locus_tag="IV038_000916"
     CDS             complement(25782..26408)
                     /locus_tag="IV038_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8341155.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26734..29109
                     /locus_tag="IV038_000917"
     CDS             26734..29109
                     /locus_tag="IV038_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP8341156.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29133..29624)
                     /locus_tag="IV038_000918"
     CDS             complement(29133..29624)
                     /locus_tag="IV038_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP8341157.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29811..30731
                     /locus_tag="IV038_000919"
     CDS             29811..30731
                     /locus_tag="IV038_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8341158.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30885..31229
                     /locus_tag="IV038_000920"
     CDS             30885..31229
                     /locus_tag="IV038_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP8341159.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31419..31919)
                     /locus_tag="IV038_000921"
     CDS             complement(31419..31919)
                     /locus_tag="IV038_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8341160.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32447..32530
                     /locus_tag="IV038_000922"
     tRNA            32447..32530
                     /locus_tag="IV038_000922"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32481..32483,aa:Leu,seq:cag)
     gene            complement(32854..33492)
                     /locus_tag="IV038_000923"
     CDS             complement(32854..33492)
                     /locus_tag="IV038_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8341161.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            33827..34261
                     /locus_tag="IV038_000924"
     CDS             33827..34261
                     /locus_tag="IV038_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8341162.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            34284..34604
                     /locus_tag="IV038_000925"
     CDS             34284..34604
                     /locus_tag="IV038_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341163.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            34745..34936
                     /locus_tag="IV038_000926"
     CDS             34745..34936
                     /locus_tag="IV038_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341164.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(35007..35264)
                     /locus_tag="IV038_000927"
     CDS             complement(35007..35264)
                     /locus_tag="IV038_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP8341165.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            35422..35685
                     /locus_tag="IV038_000928"
     CDS             35422..35685
                     /locus_tag="IV038_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341166.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            35709..35897
                     /locus_tag="IV038_000929"
     CDS             35709..35897
                     /locus_tag="IV038_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341167.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(35980..36165)
                     /locus_tag="IV038_000930"
     CDS             complement(35980..36165)
                     /locus_tag="IV038_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341168.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            36380..36751
                     /locus_tag="IV038_000931"
     CDS             36380..36751
                     /locus_tag="IV038_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8341169.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(36901..36973)
                     /locus_tag="IV038_000932"
     tRNA            complement(36901..36973)
                     /locus_tag="IV038_000932"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36938..36940),aa:Gln,seq:ctg)
     gene            complement(37019..37447)
                     /locus_tag="IV038_000933"
     CDS             complement(37019..37447)
                     /locus_tag="IV038_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341170.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(37507..38265)
                     /locus_tag="IV038_000934"
     CDS             complement(37507..38265)
                     /locus_tag="IV038_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP8341171.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            38560..38757
                     /locus_tag="IV038_000935"
     CDS             38560..38757
                     /locus_tag="IV038_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341172.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            38863..39345
                     /locus_tag="IV038_000936"
     CDS             38863..39345
                     /locus_tag="IV038_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341173.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(39403..39999)
                     /locus_tag="IV038_000937"
     CDS             complement(39403..39999)
                     /locus_tag="IV038_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8341174.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            40386..41852
                     /locus_tag="IV038_000938"
     CDS             40386..41852
                     /locus_tag="IV038_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP8341175.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        13829 a         7134 c         8483 g        12456 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagatg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccatgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa
    24061 atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag
    24121 cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga
    24181 agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc
    24241 aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca
    24301 aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg
    24361 ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca
    24421 ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca
    24481 ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac
    24541 caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac
    24601 caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac
    24661 ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac gacattgaac
    24721 atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga
    24781 ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct
    24841 ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt
    24901 ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat
    24961 aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt
    25021 ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg
    25081 ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg
    25141 cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat
    25201 cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac
    25261 caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca
    25321 atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt
    25381 gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag
    25441 ggatcgcacc ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc
    25501 taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa
    25561 tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat
    25621 tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat
    25681 cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt cgtacgatat
    25741 aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga
    25801 tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat
    25861 tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct
    25921 gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa
    25981 tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa
    26041 gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata
    26101 acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc
    26161 catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc
    26221 actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt
    26281 gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt
    26341 gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg
    26401 atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat
    26461 tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact
    26521 atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat
    26581 atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct
    26641 aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt
    26701 tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg
    26761 cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac
    26821 catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac
    26881 tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt
    26941 gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa
    27001 acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa
    27061 gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa
    27121 ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga
    27181 gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt
    27241 caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat
    27301 gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa
    27361 cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc
    27421 gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca
    27481 ataactagac aagtagctga agatgctatg acagcattaa aagatacagg agctgaagta
    27541 aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat
    27601 ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat
    27661 cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat
    27721 acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg
    27781 gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa
    27841 aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag
    27901 atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag
    27961 gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga
    28021 gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta
    28081 gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag
    28141 cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc
    28201 agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata
    28261 gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa
    28321 agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg
    28381 ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt
    28441 ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta
    28501 gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct
    28561 tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat
    28621 gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt
    28681 caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa
    28741 ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg
    28801 cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga
    28861 tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg
    28921 attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt
    28981 tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg
    29041 gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac
    29101 gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg
    29161 attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc acggttctta
    29221 cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg
    29281 atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt
    29341 tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc
    29401 acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca
    29461 tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt agaatccttg
    29521 aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct
    29581 gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata
    29641 tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg
    29701 tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat
    29761 tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt
    29821 atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg
    29881 cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga
    29941 tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa
    30001 catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac
    30061 gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc
    30121 cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag
    30181 gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct
    30241 ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg
    30301 gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta
    30361 tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa
    30421 cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg
    30481 tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga
    30541 tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg
    30601 acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg
    30661 aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac
    30721 aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca tctaataaga
    30781 acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa
    30841 aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc
    30901 aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa
    30961 tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag
    31021 aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc
    31081 cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa
    31141 cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt
    31201 atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa
    31261 taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg
    31321 tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc tattgataaa
    31381 ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt gattttcgta
    31441 tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg tgctttaaga
    31501 ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat tttttttgcc
    31561 tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata attaggggag
    31621 atgccgtaac caatatggcc agcgccgttc cttaatgttt cgttcagttt atggcgcaaa
    31681 ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt tgcgggaacg
    31741 taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac ataatctaaa
    31801 aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt tggtccaaag
    31861 ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat cagtttcatc
    31921 gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact attgttgtaa
    31981 atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc gaacaataca
    32041 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
    32101 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
    32161 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
    32221 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
    32281 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
    32341 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
    32401 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
    32461 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
    32521 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
    32581 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
    32641 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
    32701 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
    32761 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
    32821 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
    32881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    32941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
    33001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    33061 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    33121 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
    33181 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    33241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagtaga tcaggttaat
    33301 atcagaaatt gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt
    33361 ttgagaaatc gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc
    33421 agtcgttccc atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt
    33481 aagtgatgtc atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg
    33541 aggaatactt taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca
    33601 aatccacgtt ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc
    33661 acataatgca gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa
    33721 taacttgaaa gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt
    33781 atttttgctt gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata
    33841 ccagaacatc atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc
    33901 ccttgactta gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt
    33961 tgagctgacc catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac
    34021 agaagtagcc atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca
    34081 cgcaatagtt gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga
    34141 aaatattgat ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt
    34201 tggatcgacc gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta
    34261 atgagataag ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa
    34321 aaatagaaat tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg
    34381 ctttagtaaa gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac
    34441 aagataaagg attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg
    34501 ataaatttat ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat
    34561 tagataaatt aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc
    34621 agcctttaga aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa
    34681 actagaataa tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag
    34741 gcaaatggaa gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata
    34801 tgaagaagcg aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat
    34861 cgcacaagaa tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt
    34921 tggtataaca gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc
    34981 gttttctttt taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa
    35041 ataataagaa aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt
    35101 gtttcccaat gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc
    35161 agtaaaactg aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata
    35221 ataaaaaaag gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata
    35281 aataaagtcc atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat
    35341 ttatccggta aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt
    35401 gaaagaagga gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc
    35461 aataatattc gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg
    35521 aataaaataa atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga
    35581 agttattata gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat
    35641 ctagatacca aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga
    35701 ggaaaaaaat gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa
    35761 agattgatgt aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga
    35821 atcgtaaata tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc
    35881 cgacacgttt attttagtat gaagtattca gaaaagaacc aaaggaggac gttgcagcag
    35941 cattgtcctc ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa
    36001 tttgtttttg acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata
    36061 aggtcctaat tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt
    36121 cttaatcatt tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt
    36181 tgctatcaac atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca
    36241 ataatatcaa ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag
    36301 gagaaaaatc tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat
    36361 ataacaagga ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag
    36421 tggtattttg ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg
    36481 tatacttgat cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa
    36541 accagaggaa gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa
    36601 tgggaaagaa cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt
    36661 gaaaattagc aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt
    36721 tccggagaaa gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga
    36781 ttttattatt cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa
    36841 tccccctgca aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa
    36901 ttgggatggc agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt
    36961 tggctacatc ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt
    37021 atttttcttg atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt
    37081 ctaatccagc aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat
    37141 attctttgac aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa
    37201 agcgagtata ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact
    37261 cttgatctgc gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg
    37321 tagcaggtct gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct
    37381 tgcagtcacg gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt
    37441 tttccattat ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt
    37501 gaattactag cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga
    37561 aaaatcgttg atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt
    37621 ttctttgtca tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc
    37681 attatttgtg gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac
    37741 agaagtccag acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc
    37801 ggtttttgga aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg
    37861 gggaacatag acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt
    37921 aggatcgcca tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa
    37981 ctggttttta gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc
    38041 acagccattt tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac
    38101 ttctgctttc tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat
    38161 tgtatgttct ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc
    38221 tgtgccccct agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt
    38281 tcagctgttt atctttccta ttataaatgt atttttattg gaaatggaag tattatattt
    38341 taatatgtag gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct
    38401 tctaaataca acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa
    38461 ggttgatttt taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg
    38521 taatgtggag aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat
    38581 ttaggagaac aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt
    38641 gtatcaaaaa tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa
    38701 gaattattag atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga
    38761 aaacaattgg acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt
    38821 cttttcctat tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg
    38881 gttctattgt ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa
    38941 attatttatc ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca
    39001 ctagtcattt ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc
    39061 ttgtcagcaa tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga
    39121 cttgttttga atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta
    39181 ttatatcagc caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga
    39241 ggaccgatgc tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc
    39301 ttaaacgatg aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga
    39361 caagatgttt gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa
    39421 gcgatcggca tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac
    39481 taattgcgaa cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc
    39541 aataactggg ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca
    39601 agtattagct gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata
    39661 taattcaaat tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt
    39721 accataacca tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg
    39781 agtcaatggg cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa
    39841 aaggataact gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc
    39901 tttgatcaaa gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat
    39961 tgaacggcgg tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt
    40021 tgcgttttgc atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag
    40081 taaacagata tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat
    40141 cgttatgtaa ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt
    40201 atttgaaaaa aacagctgat atataattat taaatctcta tcttcttttt tttatgatat
    40261 tgccattaaa aattacttgc ttttatagaa aattagttta tatttcttaa ataagttttt
    40321 tagttaaatt tttcggtaaa gatgtataat attttctgaa aatttaaaga attggggtat
    40381 agattatgga aaaacagcaa gacaagggct tcttcgggca gccgaaggga ttgtcaactt
    40441 tgttcttcac tgagatgtgg gaacgattta gttattacgg tatgcgtgca ttacttatct
    40501 attatatgta cgatacagtt gccaacgggg gacttggatt accacgagca acagcattag
    40561 ctatcatgtc gatctacgga tctttgatct acatgtctag tatcgtcggc ggatgggtat
    40621 ctgaccgagt gttaggagca aaacgcactg tattcttcgg tggagttttc attatgcttg
    40681 gccacatcgt cttggcttta ccatttggtg taagtacatt gtttgtttcg atggcattga
    40741 ttattatcgg aaccgccttt ttgaaaccaa atgtttctgg catggtcgga catctttatt
    40801 caaagactga cttgcgaaga gatgcaggat tttcgatttt ttacatgggt gtcaacttag
    40861 gggcattatt agcaccacta atcgttggta caatcggtca agaatataac tatcatttag
    40921 ggttctcttt agctgctatt gggatgttct ttggattatt acaatacatc atccaaggac
    40981 gcaagacatt agacggagtg ggtacaacac cgccgaatcc tttgacaact gaagaacgta
    41041 aaggattcat ccgtaacttg atcatcgcac ttgttgttat cgctgcagta ttcggcgggg
    41101 cacaactaac tggtcatctg acaattgact tttttgtcaa tacaatcagt gtattgggga
    41161 ttatcttacc gttgtattac tttatcaaaa tgctgacttc aaaagatgta actgctgaag
    41221 aaaaaccaag agtatgggct tatatcccat tattccttgc agcaatcatt ttctggtcat
    41281 tacaagaaca aggttcatct atcttagctt tatttgctag tgaacgtaca caatctacgc
    41341 tatttggtgt aacgatccaa gcaagctggt atcaatcatt gaatccaatt ttcatcttta
    41401 ttctgacacc agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag
    41461 tagtgaaatt ctctcttgga cttttatttg caggattatc gtacttgcta ttgatgttgc
    41521 cgggtatgct ttatggtaca agtggaaaag taagtccatt gtggttgttg ggaagtttct
    41581 tcatcgtggt tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat
    41641 tagcaccacg agcatttgaa tcacaaacaa tcgctatttg gttcttagct gatgcttctt
    41701 cacaagcaat caatgcacaa attgcacgct tctacacacc gggaacagaa tctgcttact
    41761 tcggtattat cggtgcagta gcaatcgtcg gtggtatctt gctatttact gtgaaagaac
    41821 caatcaaaaa actaatgggc tctattcatt aatcaatgaa cataaaaaca ggaattgctt
    41881 atttgcagca gttcctgttt tt
//
LOCUS       DACDCJ010000018        38325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDCJ010000018 DACDCJ010000000
VERSION     DACDCJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.018"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="IV038_000939"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="IV038_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP8341176.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="IV038_000940"
     CDS             complement(2204..4390)
                     /locus_tag="IV038_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP8341177.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IV038_000941"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IV038_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP8341178.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="IV038_000942"
     CDS             complement(4612..5052)
                     /locus_tag="IV038_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP8341179.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="IV038_000943"
     CDS             complement(5126..5665)
                     /locus_tag="IV038_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP8341180.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="IV038_000944"
     CDS             complement(6280..7746)
                     /locus_tag="IV038_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8341181.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="IV038_000945"
     CDS             complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="IV038_000945"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8341182.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10662..11021)
                     /locus_tag="IV038_000946"
     CDS             complement(10662..11021)
                     /locus_tag="IV038_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP8341183.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11051..11392)
                     /locus_tag="IV038_000947"
     CDS             complement(11051..11392)
                     /locus_tag="IV038_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341184.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11389..12063)
                     /locus_tag="IV038_000948"
     CDS             complement(11389..12063)
                     /locus_tag="IV038_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8341185.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13240..14538)
                     /gene="asnS"
                     /locus_tag="IV038_000949"
     CDS             complement(13240..14538)
                     /gene="asnS"
                     /locus_tag="IV038_000949"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP8341186.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14578..15768)
                     /locus_tag="IV038_000950"
     CDS             complement(14578..15768)
                     /locus_tag="IV038_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8341187.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15789..16316)
                     /locus_tag="IV038_000951"
     CDS             complement(15789..16316)
                     /locus_tag="IV038_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8341188.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16363..19152)
                     /locus_tag="IV038_000952"
     CDS             complement(16363..19152)
                     /locus_tag="IV038_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP8341189.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19299..19499)
                     /locus_tag="IV038_000953"
     CDS             complement(19299..19499)
                     /locus_tag="IV038_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8341190.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19951..20271)
                     /locus_tag="IV038_000954"
     CDS             complement(19951..20271)
                     /locus_tag="IV038_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341191.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20655..21773)
                     /locus_tag="IV038_000955"
     CDS             complement(20655..21773)
                     /locus_tag="IV038_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341192.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            22244..23551
                     /locus_tag="IV038_000956"
     CDS             22244..23551
                     /locus_tag="IV038_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP8341193.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            23670..24869
                     /locus_tag="IV038_000957"
     CDS             23670..24869
                     /locus_tag="IV038_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8341194.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24896..26164)
                     /locus_tag="IV038_000958"
     CDS             complement(24896..26164)
                     /locus_tag="IV038_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP8341195.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26327..26974)
                     /locus_tag="IV038_000959"
     CDS             complement(26327..26974)
                     /locus_tag="IV038_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP8341196.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27382..27885)
                     /locus_tag="IV038_000960"
     CDS             complement(27382..27885)
                     /locus_tag="IV038_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8341197.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28008..28772)
                     /locus_tag="IV038_000961"
     CDS             complement(28008..28772)
                     /locus_tag="IV038_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP8341198.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28880..29155
                     /locus_tag="IV038_000962"
     CDS             28880..29155
                     /locus_tag="IV038_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP8341199.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29593..30543
                     /gene="yidC"
                     /locus_tag="IV038_000963"
     CDS             29593..30543
                     /gene="yidC"
                     /locus_tag="IV038_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8341200.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(30722..31696)
                     /gene="lacD"
                     /locus_tag="IV038_000964"
     CDS             complement(30722..31696)
                     /gene="lacD"
                     /locus_tag="IV038_000964"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8341201.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(31693..32151)
                     /locus_tag="IV038_000965"
     CDS             complement(31693..32151)
                     /locus_tag="IV038_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8341202.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(32148..33611)
                     /locus_tag="IV038_000966"
     CDS             complement(32148..33611)
                     /locus_tag="IV038_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8341203.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(33593..34543)
                     /gene="pfkB"
                     /locus_tag="IV038_000967"
     CDS             complement(33593..34543)
                     /gene="pfkB"
                     /locus_tag="IV038_000967"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP8341204.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            34686..35434
                     /locus_tag="IV038_000968"
                     /pseudo
     CDS             34686..35434
                     /locus_tag="IV038_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            35700..37178
                     /locus_tag="IV038_000969"
     CDS             35700..37178
                     /locus_tag="IV038_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP8341205.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(37246..38301)
                     /locus_tag="IV038_000970"
     CDS             complement(37246..38301)
                     /locus_tag="IV038_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8341206.1"
                     /translation="MLITGAAGFIGSNLVLELLRTQSSIKIIGIDNMNDYYDVSIKNY
                     RLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLAAQAGVRYSITNP
                     DMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKKIPYSTEDKVDNQ
                     VSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLRAGDNI
                     QIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHIYNIGNNTPENLL
                     DFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSALERDFEFKPSTSL
                     RDGLRKFAQWYKSFYEV"
BASE COUNT        11153 a         8143 c         6651 g        12378 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
     7981 ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaaaga ggacgttcat
     8041 tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa
     8101 cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat gcaaaaccaa
     8161 aagcacctaa ggcaagtacc aaaattttta tcccaatagc aaagacaatg ttctgtttga
     8221 cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat ggttgatcat
     8281 tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca cccattgcga
     8341 ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg acaaatgcgg
     8401 tctttttatt gtttctttgt aagatttcct ctaaccgctg cactttatct tctggaagca
     8461 attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca atttttttcg
     8521 aatcgcccgt caacatgact gtgttcttca caccagcctt ttttaattga accaatgcgt
     8581 ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa ctttgatcga
     8641 tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt tcaaatcttt
     8701 ccatcaattt gtgattacct gctaatactt gtttgccatc aacttcagct aagatccctt
     8761 cacctgcaaa ttcttggata ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa
     8821 cagattgagc aatcggatgt gtagaaaact gttctgcact cgcaacatat tgcatgaata
     8881 tctttggatc gatttttgta tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc
     8941 cggttttatc gaataccact gtctcagttt gtgcaagcaa ctccaagtaa tttgaacctt
     9001 ttactaatac accgatttta cttgctcctc cgataccgcc aaagaaacta aggggtacgg
     9061 agatcactaa tgcacatgga catgagatca ctaaaaatgt caatgctcga tagatccatt
     9121 cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac agctaataaa acggccaatc
     9181 ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat gaaattttca gctggtgctt
     9241 ttttactgct ggcattttct actaattcta ataatttgct gatagtagaa ttttcataag
     9301 atttttctac ttgaactaaa aatggtttat ttttattgat aaatccactt aatactgttt
     9361 caccaacata tacagattta gggactgatt ctcctgtaag tgctgacgta tcgactaatg
     9421 attctccttc gatgatcgtt ccatctaacg gtacacgttc accaggcttc acaagtacaa
     9481 actgaccgat ctgcacgtct tcagggttca cggtcatcaa tccttccgtc gtctgcacgt
     9541 gagctgtttc tggacggatc gccatcaatt cgcgaatcga ttttcttgaa tgactgactg
     9601 ccaatccttg gaaatactcg ccgatttgat aaaatagcat cactgctacg gcttctggat
     9661 attcaccgat caacatagcg ccaatcgtag caacagacat aagaaaattc tcatcaaaga
     9721 tttttccttt gacgatattc atcactgcct tcttcactac atcatatcct atcagcagat
     9781 aaatcaataa ataccctatc aagcgaattg gttcattaaa atcaaataat agcaataaag
     9841 cgatacctgc aagggaaaat aaaatccgga acagcgtatt tctctctttt tcatttccat
     9901 gttcagatac tttttcctta tctatttctg ttactttgac atctggttct aattccttca
     9961 ctactttttt tatttcttgt tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta
    10021 gttttccaga agtgaagttt accgttgctt ccttgactcc attgatcttc tgaactcctt
    10081 tttcgatttt cattgcacag ttcgcacaat ctaaaccttc taatctgtaa cttttcattt
    10141 taatgacctc ctcgcctcaa catatgaata tctcttcata actaattata aatgaacggt
    10201 tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct
    10261 tccatacaga tgttttggct tattctttct ttttcttttc gatcattgcc actgctctta
    10321 tccaacccgc acttgccttc ttgatcttga caggatagca actaagtttg aagcctgttg
    10381 ctggcaattt gtctaaattc attaattttt cgataggaca atatcttttt tcaatccctg
    10441 caaaatgtcc ttcccagata ttcgaagctc cactacgctt tcaaaaatat agaaagagtt
    10501 ctttaatgaa aattatttca actttccata aacagtaacc ccatataaat aaaagacgta
    10561 aaagtcacat ggaagaactt atacaggaga acgcatagag tcaggtgaaa tacatgtatc
    10621 ccacctgact ctatgcttta tctaaccttt attattctct tttatacaat ttctgcaccg
    10681 aatggcatca acgccaatcg agcaagtttg aaagattgct tggcaaaagg tatccctatg
    10741 atcgtgatac atagaaggat gccgctgacg acatgtgcga gagcaattgg taggcctgaa
    10801 acgatcagcc agatgatatt aacaatcaat gacatgccag actggtgata aattacttct
    10861 ttgccaaaag gcgcaaaaga aagggaggcg atcttaaaac attgcatacc gattgggata
    10921 cctatgatcg tgatacacca aagtatcccc gctgctaacc agctaagacc accgattagt
    10981 ccgccgaata taaaccagat gacatttcct aaacaactca ttgtatttct ccttctatta
    11041 ctgtttttga ttaaaactgg gcaaaaaatg attttattct tcttttttta gcttccagta
    11101 tcaaatattc tgcaaatttt ttttggattt cttccgtatt ttcttttgtt actaatgtgt
    11161 gttggattag ttttcctgat ggtcgctgat ccaatcgttc ttctgactgt tgcacgtccg
    11221 cttgcgcaaa tccgataatc cgtttttctt ttgttacagc aacgagcaat agtttattct
    11281 tatcatttag gtcagctgcc gtttttgctt ttacgaatga ttctggatga tcagtaatca
    11341 gctgattgat ttgaggtaga tcttctttta ctgcccaccg tatttccatc atttctcact
    11401 tttctttcgc tcatttttat ctccactaaa aaaatcgact gcggcaattg catctatcca
    11461 gctttgtgca atgatctcat gtccgccaat cgttgggtgg accccatctt ctcttgtata
    11521 tttcgaaaaa ccataaacaa tccctaaagc attgaatata ccatctaaag gaaccagcaa
    11581 tgcgccatat tccttagcaa gtttacgaac aatttggatt ttagggtcta agtctttacg
    11641 ccagccatga cggtcgtcgg ttataggaag tacaaacggc tccatcaaga tgatgttgca
    11701 gattcccact gtttgaagtt catttagtaa ttttcgataa acggattcaa attgctgagc
    11761 agatttctca gttccgaata tagcctgatc gctagtatta tgccaggtat cattgattcc
    11821 gatcaaaatc gatacatagt caggttgtaa agctaaacaa tcttcttgcc agcgtacttg
    11881 cagatctgca attttgtctc ctcctatccc acggttcatg cattcaaatt ccatcgttgg
    11941 atattgggag attagttttc cggcaatcaa aagtggataa cctttaccta gatcagttgc
    12001 ttctgctcgg tctcttcctg cgtctgtaat gctatcaccg ataaataata tacgtttttt
    12061 catttgtcaa aaactccttt ttgaataaaa cgatgatttt cttccttata ttatcccata
    12121 agccctatca agacgaaaga gaaaatatat gtataaaaaa tgacaccagc ctagtccctt
    12181 caaacaattg aatgaacgct catcaagtgt ctagtttttt atgctatttt tttcttaacg
    12241 tgccggattc cccttgattc cttcgttgtt tggtccagag agatcgatca cacctaagct
    12301 tccgtcaatc agaccactga tgacgccatc agaacatata tttataggct catctaagac
    12361 ttctagtaaa tgtcccttta ctaatatctc ttctagattg cttgggatag attggtcata
    12421 actttatctg gtccgatgat ttcaagggtg aattgaactt acatatggat tattgcatgc
    12481 tgaaaatacg gacctactcc atcttcgaat gttactatta gtttgttttc aggtgctaga
    12541 tgctgcttta tttcaacagc agaagcatca tcaaatcaga tttccatttg aattaccttc
    12601 tttgtaaatg tttgattgat tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt
    12661 tcttttgctt tcttctcttg tctgctggaa acttccttta tcccgtattt taaaaaaaga
    12721 actgcgagag ttcgcagttc ttttttagaa taaacggtgc tctccggcca actcctcggt
    12781 cacaagtcac aatagtcatc aaacatgaaa aactgttttt tgaaattact ccttgtgact
    12841 cggagctgaa cggcaggcgc acaacctctt gatataaacg gtgttcaatc agctgaccct
    12901 cgatattagc taaaaaaacg gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    12961 tactcaggtc aaggcactga tttcacaacc tcttccttaa acggtgttca aaagccagtt
    13021 tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc catttccaca aaatttttct
    13081 taatactcga aactaaacag ctttttcaca acctctccac taaacggtat tcaaaagctt
    13141 acatctgcgg taataagtcc cgccaattca aaaacatgaa cagctgtttt tgcttggctg
    13201 ttctaattac ttgatgcaga acagcttttt catcacctct tatggataaa ttcggttcaa
    13261 caaacgtggg aatggaatag cttcacgcac atggtcgata ccagcaatcc aagtgactgt
    13321 tcgttcaagg cctagaccaa aaccagagtg cggaacagaa ccgtatttac gtagatcaag
    13381 ataccatgaa tattcttttt catccaaacc gtgattacgg atttgttcta gcaagaaatc
    13441 atagtccgtt gcccgttcgc ttccgccaat aatttcaccg taaccttctg gtgcgatcat
    13501 atctgcacaa attaccacgt cttctcttgt tggatgtggt ttcatgtaga atggcttgat
    13561 agcttttgga tagtttagga taaagacagg ctgttcgaat gaattcgcaa tgaacgtttc
    13621 atgaggtgaa ccaaagtctt cgccccattc gatgtcatca aatccatttt tcttcagtaa
    13681 ttcgatcgca tcatcataag taatacgagg gaatggcaat tttgtataag tttttaagac
    13741 ttctttgtct cgtcctagta catctaatgc gtattcgcaa tgatctaaaa cattttgtac
    13801 taagaatgct acatattgtt cctgtacttc taaactttct tcttgatgag tgaaagccat
    13861 ttctggttcg atcatccaga attcaatcaa atgacgtcgt gtttttgatt tttcagcacg
    13921 gaaagtcgga ccgaatgaaa agacttttcc gaaagccata gcagcagctt ccatgtataa
    13981 ttggccactt tgagataaat aagctttttg atcaaagtaa tttgtttcga atagatccgt
    14041 agtgccttct ggtgcggaac cagtcaagat cggcggatcg attttgacaa aatgatgttg
    14101 attaaagaat tcatacgttg cacgaatgat ttcatttctg atctgcataa tcgcatgttg
    14161 tcttgaagaa cgtagccata aatgacgatg atccatcaag aagtctgttc cgtgttcttt
    14221 tggtgtgatc ggataatcgt ggctctcgct taccacttcc acatttgtaa ttccgatttc
    14281 ataaccaaat tttgaacggc tgtcttcacg gatttctcca gttactaaca cggatgtttc
    14341 ttggtgcaga tttttagctg tttggaaaac ttcttccggc acttcgcttt ttacaactac
    14401 accttggaaa aaggctgttc cgtctcttaa ttgtaaaaat gcgatttttc cgcttgatcg
    14461 cttatttgcg acccaagcgc cgattgttac tgtttctccc acatggtttt tcgaatcgat
    14521 gatttgaatt tgttccacgt atgactctcc ttttatattc cttgaatttc ttcaggatta
    14581 attttgactt ttactctcaa taaattgctt gattctgcgt accgcttctt ctaacgtagc
    14641 gcgatcagtg gcataactca tgcggacatt ttctggtgcc ccaaagcctt cgccagtcac
    14701 aagcgctacc cctgtttctt ctaaaagatc ttctacccat tgtgtgacat tttcatattt
    14761 acacatgcgt agcgtttctt ttacattagg aaaaagataa aaagctcctt gtggcttttc
    14821 aagagaaaca cccggcagtt ctgccacaag cggatataac gtattcaggc gttcttcaaa
    14881 ggcgttccgc atttcttcca ctgtatcttg ctcgcctttc aatgcttcaa cagctgcata
    14941 ttgactgact gctgtcggat tgctagttga ttgggaagca acagcgatca ttccattgat
    15001 gatcacagga tttcccaccg cataaccaat gcgccagcct gtcatcgcat atgttttaga
    15061 cacgccgttg atgatgatgg tctgattttt gattgcttct gaaatcgttg caattggagt
    15121 aaactcattg ccattataaa ctaaacggcc ataaatatca tcagcaacta tcaaaatatc
    15181 attttttact gcccattcac cgattgcctg cagttcattc ttgctataaa tcattccagt
    15241 cggattcgat ggagaattga tgattacggc ttttgttttt tctgaacgtg cttgttctag
    15301 ctgctcaaca gtaactttcc agctattgga ttcttctcct ttcaaaaaga caggaagtcc
    15361 ttctgccagt tttacttgct caccgtaact gacccagtaa ggcacaggaa tgatgacttc
    15421 atcttgcggg tcaaggatcg tttggaaaag ggtatacaaa gcaaatttcg ctccgtctgt
    15481 tacgactgtt tgtgaaggtt catagacaag tccatagttt ttcttcagat aatcaacgat
    15541 tgcctgtcga agctctggga ttcctgctgt tggtgtataa tagctagctt tcccattgcg
    15601 gattgcttcg attgctgctt cttggatgtt ttctggtgtc gcaaaatccg gttctcccac
    15661 agtcaaactc agcacatctc tgcctttggc ttttaatgcc ttggcctttg ccgcagctgc
    15721 caaagtgaca gacggttcta atcgttttgt tctttttgat aactccatcc cattcattcc
    15781 tttacctttt aaattccctt gattgtcttg acttcatctc cagattcaaa gccaatcagg
    15841 taataattca gtttcccatt tgtttctttt cctactactt cccacacagg ctgatcatca
    15901 tacatcccta aagcagcttt ttcgatttgg atttcaggat gggccgcagc aattgtctgt
    15961 tttgcttcat tttcagtcag tccctttgtt tgatccaata cttttacttt ttcaccagat
    16021 ttaggaatga taacggcgat ttcttttcct ttatcatttg tacctgtcag tgaaaagtat
    16081 gtcttctttc gagtaaacca ataaaaatga tcggcttcct caagatttgc atatttcttt
    16141 gcgatttcta ctgcttcttt cttcgcctgc accgccggac gactggaacg caagtaaaaa
    16201 atcgagctga tcacgatgac cgctaataaa aatacgatca agccaagtaa cacgcgatta
    16261 atcgtttttt cctttgtctg ttttttcttc aagggtcgtc gctcctttat cttcattcct
    16321 tttcattata gcagatttta attatgaaaa aagacagctc ctctaacttc cttcttcatt
    16381 tttctttaag aattcatgga tatcctgata gatttcctcc atagctagct ctttgatcgg
    16441 tacttttttc ggtaatgcat ggatgatccg tgcaccatat tttgcagtaa ctagtcttcg
    16501 atccaaaata agcaatacgc cacgatctgt ctccgtcctt atcaagcgtc ctaatgcttg
    16561 gcgtaacttc aaagctgctt taggtacgga ttcctggtaa aaaggattga tcccctctga
    16621 ttctaaataa gcgttcctag ctttgaccat cgggcgaagc ggattatcga agggtaaacg
    16681 tgtgactaca acgatctgca aagcatctcc aggcaaatct acaccttccc aaaaactgtc
    16741 agcgccaaac aatacgcttt tttctgacaa aatgaagcgt ttcaaaagct tctcccggct
    16801 tccgccaaac ccttgagcta gtatttctcg tcctttttct agcatagaca gatgcatttt
    16861 ctgatagacc ttctgcaaaa catcatgaga agtgaataac acaagtattg gccgatcttc
    16921 ttgttcgatc agacctgtaa gtgctgtctc taaataatcg atatatgctt gattgctgac
    16981 ttgatggacg gcaggtgcct cttttggcac atagatcctt gcctgtttct cataatcata
    17041 aggagaggca atcactttaa gaggtgctgc ttcaatgcca agccgcttag agagatagtg
    17101 tcgattaggg ccgatcttca gcgttcctcc aatataaaga atacgatcat atcgaggata
    17161 ccaaacagtc gacggcaaca tagccgcttg aaaatcatgg agatgaaaaa tcatctgctg
    17221 cgctgtgttc ccaggaacga accaatggat caaatgatct cgccagtctt ccagccattg
    17281 atccatcagc aagtactgct tgtctaattg atcaaataag ctatataaac ttgctaaatc
    17341 gatccgcatc tggtttgtcc agctttcttg gataatctca aaagtcagac gcatttgtga
    17401 ctgtagctgc tggatctcct gataaaaaag ctgcaggtga tcaatggctg tcccagattc
    17461 cagtggcagc tgatccaaca tctcttttgt cgtcatgact tcttctggat actgtccgtt
    17521 aggttgcttg ccgctcacta aactcattaa tccttctgcc atacaaatct gtgcttcgca
    17581 tagcgcagaa agttcttctc gataaatcga taaccagcgt aaggcttcat gttctttcgg
    17641 taaatagctt tgtattcggt caaacagctg atttggttct tctaattgct ggatttgttt
    17701 atggaaatag ttaatactta atcgtttgtc atttactttt tcagcgattt ccggcaaatg
    17761 atgtacttca tcgatcaaca aaaatggact agctggaagc aatggttctt ttcgctgtgt
    17821 ttcttgtatt aaaaacgcat gattgacaat caaaaaatta ctttgcctga ttttcttttg
    17881 caagaaacga acaaagtctt gttcgtaaaa tgactgattt cttgctaaaa atccagtgcc
    17941 tctgtgcgcg atatctgcaa agaatgaatg gttcaaattt gttaaatgta attcatccaa
    18001 atcaccggtc ttcgtttgcg tcagccaaac taagaccgcc atttgataca aagcatattg
    18061 tttttgttct ggcggttcca caagtgtgcc tttaaaacgc tgtaaatcaa tatagtggcg
    18121 atagcttttg ataattacag gttgtatggg ctgatctagc aattgattca gcaatggaat
    18181 gtctttttct atgatctgct gctgcaaaac tagcgaaacg gtactgatga cagcgggttt
    18241 ttcaggtgta gccaaataac tcaatggcag cagataaccg attgttttac ccattcctgt
    18301 agctgcttcg atcatcaaat cttttgattc atcttttgtg aaatggtcgt agaccgcatt
    18361 catcagtctg ttctgttctt ttcgatacat cagtgtttta ccaaacattt tttcttttgc
    18421 ttgttttttt cttggatatg tccgctcgcc ataatggacg tatatgaata gttccacttc
    18481 ttttttctgc aaagctagcc catcgacgat ttctaatgag ggatcaagtg gctctggttt
    18541 ttctttcatt tcttcaatca catgataaat gaaacgactc gtatccatac ctgtgtgtct
    18601 acttaatcgc gcaatctgct ctaacgtgat gattggcaat tttctcatcc gttcttcgat
    18661 cagcaaaaga agttgacccg tcacttcggc atcactatct gcctgatgag gattctcatg
    18721 aataaatcca aggctttctg acaaatcagc taagcgaaag cttggttccg tcggcaagaa
    18781 aatttgtgca agttctactg tatcgatgcc tggtatcttc aaactaggaa gtccgcatcg
    18841 catgagttca tggttcaaaa aattataatc aaaataaata ttatgcgcga caaatatggt
    18901 gtctgccaaa agattgtaaa tcgtttgggc gacatcttcg aaataaggcg ctttttggac
    18961 gcgttgattc gtgatccctg ttaaatgctg gatttgtttt gaaatcttcc gccctggatg
    19021 aatatcgatc gaaaaacgtg aagtgatccg acctgattca atcatgacgc agccaaattg
    19081 gatgatccga tcttcttttg gattcgtccc agtcgtttcg atatcaacga ctgcataagt
    19141 tcttggtttc aaaatatctt cctcttctcc tgcctctaat gttcttttga aattatacta
    19201 caaagtgcag aaaaatactc tcatcgcttt ttaaagaaaa tagacaaaaa cgcccagaca
    19261 cgcaaatgtc tgggcgttta ttttttagta taggttaatt aagctttagt aacgtttgtt
    19321 gcttgaggtc cgcgttggcc ttcttcaaca tcgaaagtta ctgcttggcc ttcttctaaa
    19381 gttttgaatc cgtcgccttg gatagctgag aaatgaacga atacatcgtt tccgttttct
    19441 gcagtgatga atccaaatcc tttttctgag ttaaaccatt ttactgtacc tgtttccata
    19501 ataaaaatcc tccttgtgta tcaacacata ttttgcaatt acttgaatgg tgtatatctg
    19561 gaagatgttt atttgaaaca agctaccaca gattaccgaa caaaaactac tcctatagta
    19621 tatcataccg tttacaaaaa agctagagtt tttcgacttt ttttattaaa aaaagttttc
    19681 tcagcgatta cataaagtga aaaaccgaac tttttttaaa ataaacgaca tattgttcag
    19741 ttggtgtttt tttgctctga tgttcttctt taaactaaaa aaattattct ttctgactac
    19801 ttatcggttc tgtttcaacg gtcgaggctg gggaagattc tttagtaggt ctgatgttct
    19861 catggtattt ctgatagcct atgacagtta ccagaagaac aaggcaaacc aatacgatca
    19921 acgagattag tttttctttt ttgctcatat ctatacctca taaataacag gatgatgtcc
    19981 atcatatttc tgagtatgag taaaagcaaa agctgcagct gtagaatgta agaaatgcca
    20041 gcaactttca taatcacagg tcggacagta ccaaaagtaa ccaaatcttg gctgcaaaga
    20101 ccgttcttct gtttcaggaa tcgcagaatc aagatctgtt gtctgtacat aaacattttt
    20161 gtacagacgt gtgcctgtaa taggagaccg ctgtgtttct tcagcaaaaa ctggctgact
    20221 tgttaaactt agggatagaa acagcaaaca ccataacagt ctttttttca taaagacctc
    20281 cttatcgata agtttttccc aattaaaatc atatctcaag aacgcccgtt attcacattt
    20341 tgttcataaa tgatgttttt tgtatcataa tcgtcaaaaa gaggtcgtga ccacgtcaca
    20401 acctcttttt taaacggtgt tccagaagta gcttcttcgg aaataagtcg aaatttcaca
    20461 aaaatttgag aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt
    20521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtattag ctcaaaaaac
    20581 ggaaaaatat gtgaaactat ttttccgttt gtttctctaa tactcaggtc aaaacactga
    20641 tttcacaacc tcttttaata acgaagaatc gctgtcagac tcttttcatc tggcgcatct
    20701 ttttgatcca aaataaacac tttcccgcca ttaatgatca catcatctgc taatgcattc
    20761 aacacttgac gccgatcata ttctgtttca ggatcttcac cgaaaccatc agctaagttt
    20821 gacgtagcga taaataaatg tgacactttt ccttctttcg cagctggaac gatatcaact
    20881 aactgatcga tgaatttctt atctaagagt ttattgtatt cttttgattc ttgagctgcg
    20941 agttcacttg acagcttctt cgccccttct tcgatatctc taaaggatag ctgcgcagga
    21001 gaagcagaaa tcgcaacgtc tgcacaataa tagggatttt tagcaatttt tttgaacatc
    21061 gtctgatttt ctggtaatgc aaataaataa atcggccatt gttcaggatt ttcgaaagaa
    21121 tccttcaagt acgtatcaac agcttggtaa tagttgaccc aatcgatctc tacttcttca
    21181 tcttttggat ttacgccatg gaaagttact ccatctcggg ctccgcctcc tccattagaa
    21241 gagaagttca aaccgccacc tgtcagttca gatcccaatg cagtaacgac atcaaccggt
    21301 gccccttctg gtagagatac ttcttttact tgtttgttgc tgacctcata caatttcatg
    21361 gagtcgcggt tcaatcccaa taaataataa gaatagttga actgcccatt tttgataata
    21421 gccagtaaat aaggaagatc acttacatag tattgatcgt ctaccgggat actcaaacga
    21481 tggatatagg tctggtcagc agttataatg accgctacac tcgtagttgc actacgccaa
    21541 aacgaaggat ctcctaacaa attttcaatt ttttcttgga acggggacca gtcatgttcg
    21601 gaatattttt tctcaaaacg tttctttgct tctttcgcaa aatttttgaa tttgatctga
    21661 tctttttcca catcttgatg ggcgacatgc gtattcagca tgatcgtgac gaatggtcct
    21721 ttagcgcctt ctgaagtcaa ttgtgtcaat aattctttat ccttttttgc cataaacgta
    21781 cccctcctaa aaaaatcatt cctttgtttc tatctaaagt ctaccacaac actatttttc
    21841 catacaataa aacgtatcta aaaaaataaa acggtttcta cggttttaaa tttagctatt
    21901 tttttaagtt atcttcataa tttcctcaat tatttagccc cagtcttcaa ctattcttca
    21961 tgaaatgctg ataaactaag aaaaagaata aaagaggtgg atcttatgca tttgattatg
    22021 attttcagtt tattaagtat tatcagttta tttgctttca gcgcttcccg cttattagat
    22081 gcggaaacac agcctattcc tgtaaaagta cgtaaaaacg accgctaaac ttccataacg
    22141 cgcgttctta tcctaaagtt ttttcagaaa cattgaaact ctctcccctc tctgataaaa
    22201 ttttaatttg tagcgaaaca aattaattac aaggagatcg gatatgtcca taaaaaaaac
    22261 acgtctagtc ctattattgg ctagtctcat tctttgcctt gcttcttttc taccagctgt
    22321 tgctgtgcaa gctgaagata cgttcaaagt aaatgcaaaa gcagcttttg ctgtagatgc
    22381 gcaaagcggt aagattcttt atgatcaaga tggagaaaaa accatgggga tcgcttcaat
    22441 cacaaaaatc atcggtcttt acatcgtcct tgatcaagta aaagaaggaa aactgtcatg
    22501 ggatgataaa gtttcgattt cagattatgc agaaaatttg agtatcaccc cagatttatc
    22561 gaatgttcct ttgcataaag aaaacaccta cactgtcaaa gaattattcg actctgcaat
    22621 catccagtca gccaatgctt caatggtggc cttggcagaa aaaatctctg gcagcgaagc
    22681 taaattcact gaacgaatga aagaacaatt gaaagattgg ggaatcaaag atgcaacaat
    22741 cgttaatgca tcaggattga acaactctta tcttggcgaa aaccgaccag aaggcactgg
    22801 agaaaacgac gaaaatcaaa tgtctgctca agatgtggcc atcgttgccc gccatttgat
    22861 cttggatttc ccagaaatac tggatgtatc aagtactacg acccaaatgt ttggcgaaaa
    22921 tacgcaatca ccagtggaaa tggtcaactg gaactggatg ctcccaggat tcgttaatta
    22981 caaggaaggc gtggacggat taaaaactgg tacgactgag cttgcaggtg cttgtttcgt
    23041 tggtacgatc actaaaaacg atcaacggat catcactgtc gtattgaatg cggacggcca
    23101 tgcggaaaac ccgagtgccc gcttcaatga aacagaccgt ttgatggact actgctatga
    23161 caattggtca gaaaaagagc taggaaaagc taacgcaagt attcctagtc taaaaaccat
    23221 tgatgtaaaa gacggaaaag aatcaacagt tcctgttgtg cttaaaagcc ctgtaaaagt
    23281 atgggtacga aacgatatgg atactggcaa attaacgatc acgccaacac ttgacaagaa
    23341 gcaagtcact gacaatgaga tccaagcacc tatggataaa ggtacaaaaa tcggtaccgc
    23401 tacgatcacc ttggcagacg ataaattagg ctatttagaa gacaacaatg caccttcaac
    23461 agagatcatt acggataaaa cagtggaaaa agcgaatata ttcgtgattg gctggcgacg
    23521 cgtcgcagaa tttttcggca acttgtttta attgaacaat aagaaaaaca tctttccatc
    23581 ggaaagatgt ttttttgctt tttctagttt aaataacagc ttttttcatc tattttcgtt
    23641 atactgtata agaaaaagga gttgaattta tgaatctaac cagagatctg atctttttag
    23701 ttctctatct tattggggta ttctctatcg tttattggcg aaaacagaag aaggatcctt
    23761 ccttgttctt ttacggcagt acgtggacat ttacggtaat cttaacttat tttgtcgtgt
    23821 tggaaatttt ttattcgacc ttttattttt tgattccatt gttgttcttt agtatctttg
    23881 ctttttctta tttcacagaa aaacgaaggc tgctgaatgg tcttttattc aatgttttcc
    23941 tcatcagttt cggcatctac ttgtttgtct tgctgtatga aacgcaaaat attttccttg
    24001 gcggactgat cgccttgatc acgattccat tgctacttgt ccttttattt ggtatttatg
    24061 gattgattgt ttttcttttt tggaacgggg tcacagtttt acgtagagaa tcgcattcat
    24121 tagctaatct tttaacgttg atactagcca tttttcttac actgtttctt gtttttgact
    24181 ttttcttgtt aaaatatttg ccgcaatgga taaatgcttt gtttttctgc gttcccctga
    24241 tcttgatcta tctatttatc gtattctaca actttttgac tgtctcattc ttgtatcagt
    24301 tcaaccggcc tcgatacaat caagacttca tcgttgtgtt aggcgctggg ttgatcaatg
    24361 gagaaacagt ttctcctctt ctagccaagc gaatcaataa ggcaattgct ttttatcggg
    24421 ctcaaagccg tgctacatta aatccgccca tcttattgat gtctggcggg caaggagcag
    24481 acgaaaaagt accagaggcc attgcgatga aacaatatgc catggaacaa ggaatccctg
    24541 aaagagacat tcttgtcgaa accaattcga caaccacttt ggagaacatg ctctattcaa
    24601 aggaaatcat ggatcagcaa atgaaagggc cataccgcgc gatctttagt tccaacaatt
    24661 accacatttt tagagccggt ctttatgcgt ggcaagccaa attgaaagca aatggtattg
    24721 gtgcaaaaac agctttctac tacttgccta atgcgttttt aagagaatac atcgccatct
    24781 tgatgatgaa caaaaaaaga catatgatca tttgcggatt gcttttcgca ggctccgcat
    24841 tcttatcgct gattacttta cttttctaaa aaaacaaggt gtgacaaaac atatgtcaca
    24901 ccttgttttt ttctttcatt ctcttaacta ttggcaaaaa gagtatccct tttttgaccg
    24961 ctacttgctg agcagaataa ataccgctgt tgcctgaaca cataaagctg atcaagcaga
    25021 tcataaagaa gtatactgcc gcttgtgaac cgaacaattc gatccccatc acaaagcagg
    25081 caatcggtgt atttgtcgca ccagaaaata ctccgataaa gcccaatcct gcaagaaaag
    25141 gaacagaaac atggagcaaa ggcgctagtg ctgcaccaag cgttgcacca atttcaaaca
    25201 atggtgtcac ttctccccct tgatatcccg cgcctaaaga caaaactgtg aagaataact
    25261 tcccaataaa atcatagaca tgtgcctgtc cattaaatga ttcttgaagt aacggaagac
    25321 tcaatccgag ataacgttgt gtttgcaaaa tcaatgctgc tgccacaact acagctccac
    25381 ctatcgtatt tcgccaaaca ggatgaggaa tccattgtgc atatcgcgct tttaaaaaga
    25441 cgatcgaacg gctgaatacc caaccaatca aaccaaaagc aatcccagca acaaaaactt
    25501 tgaagaacag caacatactc cattcaggaa tcacacccat ttggtaatgt agatgagaaa
    25561 caccatagct ttctgtcatg aaatttgcaa acagtgcagc aaagaagcta gggaagatcg
    25621 cttctgttcg gactttcccg attgccaaca cttctaatcc aaataatgtt ccagctaaag
    25681 gtgtgccaaa tacagaacta aaacccgcac tgattccact gataatcaag atttcccgtt
    25741 ctgatttatc caatcggaac aaacgtgctg tattatctgc taacgctccc cccatttgga
    25801 ctgctgttcc ttcacggcca acagatcccc caaataaatg ggtagttatg gttccaaaca
    25861 gtgtaagtgg aataagtcgc aacgggattt tttcttctcc accatttcct tgatcgatca
    25921 ctaagttgtt tccacgacta gaaagaccgc cgtgatacgc atagagataa gcaaaaagcg
    25981 caccactaac tggtagtaga taaaggagcc aaggatgtga tagtcggata tctgtcacaa
    26041 ctgtcaggct atttaagaaa aaagcagaca agctccccat gaatataccg agaatcgctg
    26101 tgatcaccag ccacttcagc atgtacacta aaatgcgtac tggactcggt aattgttttt
    26161 ccataaaaaa ttcctcctca ataaaaaaac gcctactaaa gaaaggggct gtccccagac
    26221 tttagtaggc gtcattagct atctttcgtt agcatttaaa ggcgaacacc atcacctatt
    26281 cacttccatt aaaagatagc atatttttct gtaaaaaaca ataaaattat cgttctttct
    26341 ttacacgttc agcaaattca tagaaaggag ccaatttcgt taactgttga ttacattggt
    26401 cactatcatg acaaatataa ttcccctttt tcgtatacgt gccatcagaa cctgctttcg
    26461 ttgtagaaag aaaaagcgaa acattcgatg ttttctgaca gattgcacag attcccttat
    26521 ttatcgttgg tgataaagta ccactaatcc cttttaattt ttcatcttcg taatagatca
    26581 tgaatttttt ctgcgtacct ggatcattcc agccgatgta cgtatgttct tgcaagtcga
    26641 tttcattcca agctggttgt ttgagttttt tcacttttcg gaataatttc tcgatttgtg
    26701 gtttgctagg ctgcataaac ggctgaacga tttgcttcag ctcctccaat gctcgttctg
    26761 cttgcgcttt tgtcagtttt acgtctaaaa ctgtcgttaa aaatgtatcg atttctggca
    26821 gttcgtgttc taatatgtct cgaatctttt cagttgctag tgcctgaact gtttgtaccg
    26881 tttttggatc attgacagat gagtacgcat ttaataactg ttctacttct ctttttataa
    26941 aaaaatattg gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca
    27001 aagtgcatac tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc
    27061 ggactctttt aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattct
    27121 tctatgtaca tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac
    27181 tgcaaacaga aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat
    27241 ggtgttcaaa agcttgcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagta
    27301 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg
    27361 gtctattatt ggttttacct gttaaatcga ggattctttc ccaaagtaac gttgccatct
    27421 cttacgcata cttgcagaca tgggctgagc cgcttctttg actgcgcagt atttgtccac
    27481 gaatgtcaca atatatcctt ctttatactt aggcggtgcg atcgttgcac cccacatgtg
    27541 cttcaaaatg atatctcgct caagatccga caaatcggtg attttttctg cattctttac
    27601 agcaattctt ggatggatat acgcatgtgt cccctcgtca aatttcgtcg tacgccaatc
    27661 atagtagaac agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca
    27721 tttttttgcg agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga
    27781 atgaacatgc tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttg
    27841 tacataagac atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc
    27901 acctttttga atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt
    27961 attctagtcg ctctagataa aatagctaaa gcttaatagt tccttaatca taaagaaaaa
    28021 tgataaagaa cgatccctgc tgcgactccg acattcaatg attccgcatt tcctttcatt
    28081 gggatatata cgttctgatc agtcaaagac aagatttcct gacttactcc ctgaccttcg
    28141 tttcccatga ttatagcgaa atcctctgtt ttttctactt tgtctaatgc atgtgcttct
    28201 tcatttaatt ctgtaccgta caccgtaaac cctttgcctt tcattacagg gatcacttct
    28261 gccaaatctt tttgtaacac aggcaaatga taattgcttc cctgcatgct tcgcagcacc
    28321 tttgtactat aaatatctgc cgttccttta cctaataaaa caccagccat accaaacgca
    28381 tctgccgtac ggatcatcgt tcccacattt ccaggatcct gtacattatc tagtaaaagc
    28441 cagcctcctg aaaaatctgg cgtatcttca gatgttaacg tcatcaccgc taagatccct
    28501 tgcggtgtag gtagatctga catagcggtc atgacctcat ctgaaactag aaaagtagaa
    28561 atctctttgt ttacagccag ccagtctccc cattctttca tacctctctg tgtcacaaaa
    28621 agttctttta tttctgcatc agacgacgca gcttcttcga tcagatggaa cccttccaaa
    28681 agatattctg ccttttcttc tctgtatttc tttttatgta gcttttttaa ttctttgatt
    28741 tttggatttt ttgctgattg gatctctttc acattccttc acctcacctg attatatcat
    28801 ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattgat ttataaaaca
    28861 ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaat gtccaaggac gtgtccaggg
    28921 cgtgggattt cgctatatga ccaagatggt tgctgatcag ttaggcgtga ccggaacggt
    28981 aaaaaacgaa gacgacggct cagtcacaat cgaagcaatc ggaaatgacg acatcatcca
    29041 aaaatttatc gaagaagtca agaagtctcc gagcccaagt ggacgagtgc agtatgtgga
    29101 tgtccaagaa gatccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg
    29161 tgaaaaggtc gcttagtttt gagtattaag gaaaattttg tgaaaatggc tttccacatt
    29221 tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgtcg atcaggttgt
    29281 ttttgaggcg ctttgcctca agtattaaag ataggtagta aaaaagctga tctataactt
    29341 gcaataaatt tgcttaaccc atattttcca gacagaacaa aaaagttttt agttttttat
    29401 ataagaaaga aacacttgga atcactcttt tctttccaag tatttctttc ttgatttttt
    29461 gtcttttttc aaaatttttc gctacgaaaa agaaagaata tttatatgaa atttctatga
    29521 agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag
    29581 aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc
    29641 ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcagga
    29701 atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat
    29761 ttcaattggt cttacggctg ggcagttatt gcaatgactg tgatcgttcg tatcatcatc
    29821 ttgccactag gtatcagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg
    29881 ttaaaaccac aagttgaagc agcacaacaa aagctaaaag ctgcaacaac acgtgaagaa
    29941 caaatggcag cacaagcaga aatgcagcaa gtatatcgcg aaaacggatt gagtatgacc
    30001 ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtatttt
    30061 acggctcgct acacagaagg tatccgtgaa tcatcttttt acggcatcga tctaggctca
    30121 ccaagtatgg tgcttgtggc cattgcaggg gtcgcttatc tgcttcaagg atatatttca
    30181 acaatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct
    30241 gctatgatcg tcttcatgtc gatcagtgcg ccagccggcg ttacacttta ctgggtagtc
    30301 ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgcttg
    30361 aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt cactccaaga
    30421 aaagacgtga cagaatcagc aaaaccagct gacaacaaac aagcaacaaa acaaatcaaa
    30481 caaccaaaaa acagtacaaa taacaaaggc cgcaacgcag gaaagcagca gaagcggaag
    30541 tagatagtaa ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg
    30601 accaagattt ttgaaaaaga aatctagtcg atatttttat ttaagtgaat gtataatttg
    30661 aatcatcgat tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt
    30721 cttatctatc aacgtagaca ggtgttgctg tttcttttaa tagtgtactt aattccttaa
    30781 tattttgttt tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa
    30841 aaggtgccac tcccttcgcc catgttgctc gcccacataa gacaccattg aatgtcgaac
    30901 ctgattgttt tgcaaaacgt aatgtatctt gaaataacgt agcattgact cccgcactta
    30961 aaaaaataaa cggttgttca gtcactttcg attgttcata gaaatacgcg gatgcctctg
    31021 cttgactata acaagtctct tgcccatatc cttccacaaa tctcatattc actggcactt
    31081 ctactttcaa tacatccact tgataacgtt cttttgaaaa ttctcgcatg gcttcaataa
    31141 ctttatgggg ttttactttt gcataggcaa gagaggacgg atcgccaata gttgcatcat
    31201 aagaaaggat ctctaaaaag aaaggtatgc cttctgcatc gcattctgaa ccaatccgtt
    31261 ctataactat ttgtttttgc acattaatct ctgctggttc atccacgtca tagtacaata
    31321 aaaatttaca agcatccgca ccagcttctt ttaacctttt caccgaccaa atattcaata
    31381 aatcaggtaa tcttcccggc attttcgtat catagccggt tttttcataa gctagcagca
    31441 agccacattc tttctctcta agcgctgctg caggcaggcc gcattctgga tcaagtaaaa
    31501 tagctgaggc atagggagtc aggtattgag aaaccatctg tttaaattgg atgatgtctt
    31561 ctgatgtagc ttctttatat tggttgatca ttttcttcaa agcaccacgc tggtcgattg
    31621 ctaacgcgct aataattcca gcttcatccg tcaatcgctt caaacgttcc tgcttttcta
    31681 tactcatctt cttcatagat cttcacctat ttctttagtt aatattgtgg ctatttctgt
    31741 ttttgatttt gcttttctca actgttctgt aacctctttt cgcatcaatg atcgtgcaac
    31801 gagagacaat aatttcaaat gagtcgttcc tgcttctgaa tctggaataa ataacgcaaa
    31861 aataaaatca actgactgtc tatctaaact gttccattca attcctttgt tcagtgtgac
    31921 gataatcata ctaggttcgt tgattgtttg agacttcgca tgagggatag caaaacctga
    31981 cattattcct gtagtacctt ctttttctct ttctagaagt tttcgatata cttctttttc
    32041 gtcatgagcg attcctaaac agaatgtttg ctttgctaaa aacttaaatg cttcttcttg
    32101 actatctaat ggctgttgta aaaaaatgtt ctcttcatga atgattgtca tgataagcct
    32161 cctgctcttc cttcttcgat aactgtacga tttgctgctt tattatccat cttcttgtct
    32221 ttatcagtta ctcttttctt ttggatcaaa cctagtatca ctccaccaac gatcgaacca
    32281 ataagaatcg ctgtaaccca ttggattttt cccgtcacta caggtaacac taaaaaaccg
    32341 ccatgtgggg ctggtacttg gacaccaaac agataggtca agacagcagc aattgatgag
    32401 cctaacatca tcgttggtaa tactcggatc gggtctttcg cagcaaacgg aatagctccc
    32461 tccgtaatat gagttgatcc taaaataaaa ttaaccaaac ctgcattacg atcattagta
    32521 tcaaaatatt taccaaacaa taaagttgca aaaccagtaa tcaacggtgg tgcaatgcaa
    32581 gctactgaca cgccagccat aaaagtcgtg tttccttgtg ccaaaagagc tgtacctgtt
    32641 acatatgccg ccttattaat cggtcctccc atatcaaacg cacacatgca accaacaatc
    32701 acgccaagaa caagtggatt cgaatgttca aatccagcta aaaagctcat cattccttca
    32761 tttatagcct tcattggacc cgccagcaaa gacatcacga tgccgataaa cactacacca
    32821 ataactggat ataagaagat tgccttcaag ccatctagtt gtttaggtaa aattgtcagt
    32881 agcttttgca aaaataagat aaaataacct gctaagaacc cagctaaaat acctactaaa
    32941 aatccagtac cgccactcat ggccacgatt ccaccaacaa atccaacgac taaccctgga
    33001 cgtttcgcga tcccttctgc aatataagct gctaaaactg gaaccatcat ccccattgct
    33061 gcatcgccaa ccgtttttaa catcgccgct gtagcattgt attgtgcatg gtctggatca
    33121 aaagaataga ttccccaaac ggcaaatgaa atagcaatca atactccgcc agctaccacg
    33181 aaaggcaaca tatgagagac gccattcatt aaatttttat agatgctgcg accaaaatta
    33241 gcaccactta tcgtttctga ttctaaagta tcaacagctt cattatctgc taagatggtc
    33301 ccttttcctg ccaatgcttc gtcaatcagc cgttgtgcat ccttgattcc tgtactgaca
    33361 gaaacatcaa tgactcgttt tcccgcaaat ctttctgaat ggacatcttt gtctgcggca
    33421 ataatgacag catctgcctg tttgatttct tcagccgtta attcattctc gatcccaatt
    33481 tgtccatggg tctccacttt gatcgtgatg cctttttttc tagctgcttg ctctaatgcc
    33541 tcttgcgcca tgtaggtgtg tgcaattcct gtcggacaac cggttgctgc aattagttga
    33601 tattttccca tctttcgaac ctctcttcct tgattttgat ttgtttactt aattcctcca
    33661 catccaaaaa atcagtcagt cctttacgaa aagcagtaga gctgcctgct gctatgcttt
    33721 tacgtaacgt ttcatcaagc ggtcgtcgat tcatgtatcc tactaaaaaa gtgcctaaca
    33781 tggcatcacc tgcacatgct gtattgacaa cttttccttt tggcgagttt cctcgaatca
    33841 ccctgtcttt cgtaaaaaga atcgctcctt tctcaccaag ggataacaaa acattttctg
    33901 cacctgactc taccagtcgt tgtccataaa caaagtaatc ttcatctgta accatttttc
    33961 gtccgaacca agaagaaagt tcttcctcat tcggtttcag caaaaatggt cgatagggca
    34021 aacaatccat gacctcttga tcactgctat caataatcaa atgaatctcc ttttcctgac
    34081 aaatccgact gatttccact aaaaccttcg gagacattcc tcttggtaaa ctcccagaaa
    34141 cacaaagata atcctctgct tgtaatgctc ttatttgatt taaaaaatta tggacagctg
    34201 cctgcggtat ttcaggtcct tgattcacta atttgtattc ttctctcgtt tgattgactt
    34261 gtgtaaatac attgatacgc gtcataccag gcacttcaat aaactctgtt tggatttgtt
    34321 tcttctgtaa atagtcttct atatattttc ctgtgaaacc agcagaaaaa cctaatgccg
    34381 tacttgggaa atctagcatc tttaatacta gtgacacatt tactcctttt ccgtttgcct
    34441 gaatgtcatc ctcctttgta cggtttacca taaaaggatg catctcctct gtttcaataa
    34501 atagatcaat tgccaaattc atcgtgcatg tgtagataga cataaccaat gacctccttg
    34561 aatctattat gaaatacgcg aaagagaaaa tgttttctta atcgttccaa cacaaagaaa
    34621 acctttacat ttataaacac atcgtgctat tttttaacta acttttagtg aaagaaggaa
    34681 agaatatgga tcacaaatta agtgaaacag aacagtacct ctggaacttt atcgaacacc
    34741 acatcctaga gatccctaac tactccatcg tgaaactaag tgaacaagca aatgtctcaa
    34801 cagccaccat tgtgcggaca atgaagaaaa aaggctatga aggatttact tcgtttaagc
    34861 accatctaaa agaaggagaa aacaagaaca tcaatttttc tttttagata aagtagacaa
    34921 aggtatccgt cgagccattc taaaaaatga acaagaagtg gtacgtacga tcaatatgtt
    34981 ggaaatcggg aacattgaag atgcaataca aagaatcaaa tttactgacc gggtttttgt
    35041 ctttgcccga ggattttcag aaatgattgg tcaagagatg ctcgtaaaac ttcaattaac
    35101 aggaaaaaat tgtcaaatgc atactgatcc agagattatc aaaagcatca gtcggaaact
    35161 aactaagaaa gacagcgtta tttttgtttc tttgaacggc gagacaaaag aactcgtcac
    35221 cgccgcaaag aactgctatg atgcagagat tggttcgatt ctagttacag ccaatcattc
    35281 ctctacactc aaagattatt gcgagataaa tctcgtaggt ttcaaatcag aaggttctta
    35341 tttccctgat tatgaagtac gttcacgctt gcctctccag atcatcgcga gaatcctgct
    35401 tgacgcctat gtattgcgta tgagggagaa ttgagaagca gtttattatg gttggttaca
    35461 gtttaaaaca tcacattttt tattctttat attgtatgga ctcacgtaaa ctttttatgt
    35521 ataaagttat tcacaatcta taaagttatt ttaagtaaca aaaaataatt gaaaatactt
    35581 aaaatcaaaa aatatctttt taatcaataa aaaaattgac agttttatta ataataagct
    35641 attctgttat agaagaaaaa attgtttgtt attattagca acaaaaaaag gagtgcatca
    35701 tgaaaaagtt atctcaaaaa cgtttagctc aaatcattat tgattctaga aaatctctga
    35761 aaatgacaca acaacaactt gcagtagcta ctggtatcaa ccgtgcctta attagtcgag
    35821 tagaatctct cgattttatc ccgtcaatac aacaattaga atcattagca gaagttctaa
    35881 attttgaacc aacagatcta tttataaatg aatcaaataa atcaaacaga acaaaacatc
    35941 agtcgaagaa tattgctgta gccggtactg gttacgtagg tctttccatt gctactcttc
    36001 ttgcccagca caatcaggta acagcagtag acattcttcc tgaaaaagta aaacaaatta
    36061 acaataagaa atctccaata caagacgatt acattgaaaa atatctagct gaaaaaaatc
    36121 ttaatttaat tgcaacgact gatggtgcag ccgcctatag agaagccgat tttgttgtca
    36181 ttgctgctcc aacaaattat gatagtaaac aaaattattt tgatacttct gctgtagagt
    36241 cagtaatcaa attggtgtta gaagtcaatc caaaggcaat aatggtcatc aagtcgacgg
    36301 tacccgtagg ctataccgaa agtattcgta aaaaatacaa tactaaaaat attctattca
    36361 gcccagaatt tctcagagaa tctaaggcat tatatgacaa tctatatcct tcgcgaatta
    36421 ttgtcggcac ggaccaaact gacaaaactt tacgagaagc ctctcacact tttgcagatt
    36481 tactccaaga aggagcaatc aaaaaagata tcgaaactct tttcatggga ttcacagaag
    36541 ctgaagcagt taaattattc gctaatactt accttgcact tcgagtatct tacttcaatg
    36601 agctggatac ttatgcagaa atgaaaggac taaatacaca agaaatcatt cacggggttt
    36661 gcttagatcc aagaattggc acacattata ataatccatc ttttggatat ggaggctatt
    36721 gtttaccgaa agatacaaaa caacttctca ctaactataa cgatgttcct cagaatatga
    36781 taagtgcaat tgtcgatagt aatcgtacaa gaaaagactt tatagcagat cgagtgttag
    36841 aacttgctgg cacgtatgag gctaacagtt cgtggaatgc agaaaaagaa aaagaggtag
    36901 tcataggtgt ataccgtctt acaatgaaga gtaattcaga caacttccgt cagtcatcta
    36961 ttcaaggagt tatgaagcga attaaagcaa agggggcaac tattgtaatt tatgaaccaa
    37021 cacttgaaaa taacactaca ttttttggtt caaaagtaat aaataattta gatgatttta
    37081 aaaatatcag tcacagtatt attgctaatc gttacgatag taatcttgat gatgtaaaag
    37141 aaaaagtata taccagagat ttatttcaac gagattaaaa aataacacct cagatttgta
    37201 gtgtctgagg tgtttctata aacttttata agtaaaaaaa tacaattata cttcataaaa
    37261 agacttatac cactgcgcaa atttacgaag cccgtcacgc aaacttgtac ttggtttaaa
    37321 ttcaaaatct cgttctaacg cactagtatc tgcataagta actggcacat ctcctggttg
    37381 cattggaact agttcggtat gagattcaaa gtcgtaatct tctggtagca cccctgctgc
    37441 aattagctct tgttgtagaa tatctacaaa atctagtagg ttttccggcg tattgttacc
    37501 aatattataa atatgatatg gtgggattgg tagcccatct tccccatcaa ttttctctgg
    37561 agcgcactgc ataaccctct ttactccttc aacaatatca tctatatagg taaagtcacg
    37621 cttgcaattc ccataattga atatttggat attatcacca gcacgaagtt tatcagtaaa
    37681 accaaaataa gccatatctg gacgaccagc aggaccatat actgtaaaga aacgtagccc
    37741 tgtagacgga atattgtaaa gtttagaata tgcatgtgcc attgattcat tacttttctt
    37801 agttgctgca tacaacgata cttgattgtc tactttatcc tctgtagaat aaggaatttt
    37861 cttattagta ccataaacgg aagacgatga ggcatacact aaatgttcca caccagattc
    37921 tccattatca tatgaatgac gacaagcttc aagaatatta tagaatccta ttatgttaga
    37981 ttcgatatac atatctggat tagtaattga ataacggact cctgcctgag ctgctaaatt
    38041 caccacgata cttggtttat aattatcaaa gaggaaatca attgttgctt tatcagcaat
    38101 agaacctttg ataaaattcc aagtagatcc tggatggttc tttgcctccg tttctatctc
    38161 tgataatcta tagttcttaa tagagacatc atagtaatca ttcatattgt caatgccaat
    38221 gatttttatt gatgactgag tacgtaataa ctcaagaacc aaatttgatc caataaaacc
    38281 ggctgcgcca gttattaata ttgttttatt ctgtaaagat gtatt
//
LOCUS       DACDCJ010000019        35307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDCJ010000019 DACDCJ010000000
VERSION     DACDCJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.019"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..436
                     /locus_tag="IV038_000971"
     CDS             278..436
                     /locus_tag="IV038_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8341207.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            508..1734
                     /locus_tag="IV038_000972"
     CDS             508..1734
                     /locus_tag="IV038_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8341208.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(1823..2215)
                     /gene="rpsI"
                     /locus_tag="IV038_000973"
     CDS             complement(1823..2215)
                     /gene="rpsI"
                     /locus_tag="IV038_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP8341209.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2231..2674)
                     /gene="rplM"
                     /locus_tag="IV038_000974"
     CDS             complement(2231..2674)
                     /gene="rplM"
                     /locus_tag="IV038_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP8341210.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3193..3660)
                     /locus_tag="IV038_000975"
     CDS             complement(3193..3660)
                     /locus_tag="IV038_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP8341211.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3780..5318)
                     /locus_tag="IV038_000976"
     CDS             complement(3780..5318)
                     /locus_tag="IV038_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP8341212.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLSGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5398..6084
                     /locus_tag="IV038_000977"
     CDS             5398..6084
                     /locus_tag="IV038_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP8341213.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6285..6812)
                     /gene="msrA"
                     /locus_tag="IV038_000978"
     CDS             complement(6285..6812)
                     /gene="msrA"
                     /locus_tag="IV038_000978"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8341214.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6896..7573)
                     /gene="pgmB"
                     /locus_tag="IV038_000979"
     CDS             complement(6896..7573)
                     /gene="pgmB"
                     /locus_tag="IV038_000979"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP8341215.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7570..8700)
                     /locus_tag="IV038_000980"
     CDS             complement(7570..8700)
                     /locus_tag="IV038_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8341216.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8700..10883)
                     /locus_tag="IV038_000981"
     CDS             complement(8700..10883)
                     /locus_tag="IV038_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP8341217.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10880..11905)
                     /locus_tag="IV038_000982"
     CDS             complement(10880..11905)
                     /locus_tag="IV038_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8341218.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11950..12777)
                     /locus_tag="IV038_000983"
     CDS             complement(11950..12777)
                     /locus_tag="IV038_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8341219.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12774..13841)
                     /locus_tag="IV038_000984"
     CDS             complement(12774..13841)
                     /locus_tag="IV038_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP8341220.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13867..14703)
                     /locus_tag="IV038_000985"
     CDS             complement(13867..14703)
                     /locus_tag="IV038_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8341221.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14700..15569)
                     /locus_tag="IV038_000986"
     CDS             complement(14700..15569)
                     /locus_tag="IV038_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8341222.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15639..16922)
                     /locus_tag="IV038_000987"
     CDS             complement(15639..16922)
                     /locus_tag="IV038_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8341223.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16936..18621)
                     /locus_tag="IV038_000988"
     CDS             complement(16936..18621)
                     /locus_tag="IV038_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP8341224.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18896..19921
                     /locus_tag="IV038_000989"
     CDS             18896..19921
                     /locus_tag="IV038_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8341225.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20516..24169)
                     /gene="rpoC"
                     /locus_tag="IV038_000990"
     CDS             complement(20516..24169)
                     /gene="rpoC"
                     /locus_tag="IV038_000990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP8341226.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24248..27865)
                     /gene="rpoB"
                     /locus_tag="IV038_000991"
     CDS             complement(24248..27865)
                     /gene="rpoB"
                     /locus_tag="IV038_000991"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP8341227.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28593..29561
                     /locus_tag="IV038_000992"
     CDS             28593..29561
                     /locus_tag="IV038_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8341228.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29579..30484
                     /locus_tag="IV038_000993"
     CDS             29579..30484
                     /locus_tag="IV038_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341229.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30481..31287
                     /locus_tag="IV038_000994"
     CDS             30481..31287
                     /locus_tag="IV038_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8341230.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31444..32412
                     /locus_tag="IV038_000995"
     CDS             31444..32412
                     /locus_tag="IV038_000995"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP8341231.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32729..33253)
                     /locus_tag="IV038_000996"
     CDS             complement(32729..33253)
                     /locus_tag="IV038_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP8341232.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33540..35294
                     /gene="scm"
                     /locus_tag="IV038_000997"
     CDS             33540..35294
                     /gene="scm"
                     /locus_tag="IV038_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP8341233.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSS
                     TTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVI
                     LLVIGLRITFMSYKKSKR"
BASE COUNT        10149 a         7518 c         6609 g        11031 t
ORIGIN      
        1 atattgatct ttgataatta gatataattt ttgaataacg ttccctgcta ttcgagctag
       61 aaatacaagt acgccatgac ctagttagcg acaactactt agagatttct tttgttctgg
      121 gacaacaaaa tgccatgaca atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa
      181 actggggaga ggtgaaattc ctatgtgctt acccaaagcg gttcaaaaat tttaaactat
      241 tattttagga gatgatttta taaaatcgaa agcctacttg aataaaaaac aagcctgtga
      301 atttctagga attgataata atactttaga taagtggatt cgtgtatata attttccagt
      361 aataaaaatt gatggcgtag ttcgctttga cgtagaggat atcaaagatt tcatgaatac
      421 attcaaaaaa aattgaaatt ttcttctcgt cacatatact ataaatagcg aaatgtactg
      481 ccttactttt taaaggagtg aggaagtttg cctacaaagc tttcaaatgg gaaatataaa
      541 actaatctga ggtatccaaa aaaattcaga gaaatcactg gaattgcatc agagaagtat
      601 caaaaagtat ttccaactag gcaattagct attaaggccg aaagggaaat gaaaaagaag
      661 attgaaacag ttttaagaga ggagaatgca aactcactag aattgaaagg aaaaataaaa
      721 tttaaagatt tttatgaatc aaaatggctt cctagatatg aactaggtca aactactagg
      781 tcaaacaggg ctccaagtaa tgtaacaatt agtaatacta aggatatatt ccgattgcac
      841 atccttccaa tgtttggtga atatgcaatg aattatctga attcaaatac tgagataata
      901 tcagatgaat taacaaagaa atcgaaagaa tacgcaaata taaaaattat caaaggctat
      961 gttcgctcta tgtttgatat tgccgaaata ctatgttata tcgaattcaa ccgaactacc
     1021 aaaattattc aatgtattac cgcgcctaag aaaatggctt tagaagaaaa aaggataagg
     1081 gaagggaatc aagcattatc atcagaagag ctgactgatt ggatagaagc agtaaacaat
     1141 gacttaaata atcacttgct tacacttcac gattacacac ttttcatgct taccttatat
     1201 ctaggtgata gaaaaagtga gacttatgct ctgcaatgga aatatattga ttttaataaa
     1261 aatacggttc gtctcaaata tgctttagat aaatatcaaa gaaagacaca tacaaaaggt
     1321 tggaaagaca ccgttattca ggcaccagaa gttgtgatga cattattaag agaatggaaa
     1381 tcagctcagg ctgaacaatt gttgaaacta aaaattatac aaacacctga ccagtacctc
     1441 tttacctatt ccaaaccttc tggagaagta aattgtcctg ttcacgctga ctacttaaac
     1501 tatcgaatca atgcaataaa aaaaaggcat cctgaattgg ctcaattatc tccacacaaa
     1561 cttcgtcata cctatgcaac tatagctagg gaaggtggag caaatatgaa tcaaatctct
     1621 aatgctttaa cgcactcgga tatttctact acaaaaatat acgtgaacac acctgacgta
     1681 gttgataaat ctgtttttga agcatttcaa aaaggattga ataagtgtga ttaacatgtg
     1741 ttctcaaatg tgttcttatt ataaaaaaag acactttcca aataattgaa aagtgtcctg
     1801 aaatattgat ataaaaagaa tattaacgtt ttgagaattg tgaagcttta cgagcttttt
     1861 taagacctgg ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt
     1921 taagagctaa acggaagtca ggatcaactt ctagcaatgc acgagcgata ccatgacgga
     1981 tagctcctga ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc
     2041 cttttgtttc agtaacagcg aaaggttgat tgattacttc acgcaagtca gcatgtggaa
     2101 tgtattcttc aacatctttt ttatttacag taattttgcc agttcctggt actagacgta
     2161 cgcgagcaac tgcgttttta cgacggcctg tgccgatata ttgaacttgt gccaatgaaa
     2221 ttccctccta ttagattaag tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg
     2281 gatgttctgc tccaccgtat acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt
     2341 taggaagcat accttttaca gaagtttcga tcaaacgacg agaattttta gcacgcaatt
     2401 cgccagcagt gattgatttc aatcctcctg ggtagttgct gtgacggtaa taaattttgt
     2461 cagtcgcttt tttacctgtt aatttcactt tgtctgcatt gatgacgatg acgaaatcgc
     2521 cagtatccaa atgaggtgtg aaagttggtt tatttttacc acgtagtaca gatgcaacaa
     2581 ctgttgaaag acgtcccaat ggaacatcag ttgcgtctac tacataccat ttacgttcta
     2641 cttcgcctgg tttggccata tatgttgtac gcacgttatt ttcctccaat ttaagttctg
     2701 atgtttgaca tacacttcga gtttccgggg ctctttgtgg ggcaaacaat accatttaac
     2761 atattacccg ctaatctagt caatgtcaac gtttttctgc ttgattgaaa aatttatatg
     2821 aaactttact tcttttcttt cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac
     2881 aaaaagaggc tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa cggtgttctc
     2941 ctgccaactc ctcggtcaca agtcacaata tccataaaac ataagaagct gttttttaaa
     3001 attgtttctt gtgactcgga gctgaacggc aggcttacaa cctcttggta aacggtgttc
     3061 aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgacg
     3121 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt
     3181 caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt ggatcatcca
     3241 gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg gatcgtcgcc
     3301 ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac gatagcctgc
     3361 aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt catcagggat
     3421 ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa
     3481 gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat ccatcattgg
     3541 gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa caacaacaga
     3601 catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt ggaatttcat
     3661 ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc aatgaaaagt
     3721 acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg taaagccaat
     3781 catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata agtctgtgct
     3841 aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt atgatcagtt
     3901 tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact gatactttgg
     3961 atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc ttctgtgaca
     4021 gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa aatcggagat
     4081 tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca aactcctttt
     4141 aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat ttctttttct
     4201 gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca aagcggtgct
     4261 cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg cacaaaagcg
     4321 atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga ttgaacgact
     4381 tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct agtcgctcct
     4441 cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc tggatggaac
     4501 tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc
     4561 gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca
     4621 aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag acctgttact
     4681 tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc
     4741 acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga agctgttccg
     4801 tagtcgctga tcagttcgcc agacaatcta taaaagatat agtctttgat accgacaaaa
     4861 taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat tttgttcaat
     4921 ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc aagcacggta
     4981 tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat cattcgagat
     5041 aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga gaaggagaca
     5101 aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac ggtgcagaca
     5161 ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg gatcaaaggg
     5221 tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt
     5281 tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca aattttatgt
     5341 atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg agatggcgtg
     5401 ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg
     5461 attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg
     5521 ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt tcgctgccgc
     5581 tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg tggtggtctt
     5641 tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat atggatgctt
     5701 tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc tcacacgcta
     5761 ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt ccaatggcaa
     5821 agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca atcctatctc
     5881 ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct agaagagttg
     5941 cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt ttactttatc
     6001 tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag tgcgttgctg
     6061 attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg
     6121 atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag gttcacaacc
     6181 tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat gctggtttta
     6241 caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt cttgacgccc
     6301 caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt gtaatggagc
     6361 ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc gcttcttggt
     6421 tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa
     6481 cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg ataagaatca
     6541 ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca atagatttcc
     6601 accaactgat cataagaaat aacttctgga tcatattcga tctctactgc ttctgtatgc
     6661 cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc gccagtataa
     6721 ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat acaccaaaag
     6781 catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt ctcatcatac
     6841 cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga aactattata
     6901 tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat
     6961 caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc aatacatgca
     7021 tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc aagcatgcat
     7081 ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca ggttttgctt
     7141 ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc gttttttcta
     7201 aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt gtttttttca
     7261 agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt ttttgaatcg
     7321 cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt tctgagaatt
     7381 tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg gatattcctt
     7441 tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct aaaaccagcc
     7501 acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg aatagtgctg
     7561 ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt
     7621 tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc
     7681 tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt cgcttccttt
     7741 accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc tagcaatccc
     7801 aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta cgattacgag
     7861 gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt cgatgccaga
     7921 gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac cagcactact
     7981 gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca tggcttggtc
     8041 agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc
     8101 ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg ctttaatcag
     8161 ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt ctcccagtgc
     8221 ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg ccatgccgat
     8281 gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa caccttgttg
     8341 caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg gctgttgttg
     8401 gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg cagtttgacc
     8461 gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga ctgctgtttg
     8521 ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc catcgcctat
     8581 aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt gaatggcttg
     8641 tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca gaatacgcat
     8701 tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc tccattgaca
     8761 aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt tgtcaaaaca
     8821 aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga
     8881 agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa accaaatata
     8941 actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga tgaatgcgga
     9001 tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca ttccatttct
     9061 tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc gtggattgtt
     9121 ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata ctcaggtaat
     9181 aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt tacttcgcta
     9241 cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt ggtcaaatca
     9301 ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt gttaggcact
     9361 ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg atcgccagct
     9421 tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata tgcattattg
     9481 tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa atgatcatcg
     9541 acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt
     9601 gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc ataagcaagg
     9661 tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt tccggttttg
     9721 atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg
     9781 aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc taatcgatca
     9841 taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa gaaaggcagg
     9901 ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt tagtcccttc
     9961 gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg
    10021 aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg atgctgatgc
    10081 caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact aacgcttttc
    10141 tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt agagagcacg
    10201 atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa ggaagcggtg
    10261 atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc aatcccaata
    10321 tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc aaatactcgc
    10381 agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc gatacccgtt
    10441 ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag ggctgcctga
    10501 tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt tccttgagca
    10561 gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc ccgctcgtat
    10621 gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat ttcccatctg
    10681 atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg gttatataca
    10741 ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct tcctctgata
    10801 ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata ctctaaatca
    10861 aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc gaacgatata
    10921 aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc atctgagtct
    10981 ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg tcattaactg
    11041 gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt tcgcctggat
    11101 acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct tctctgatat
    11161 tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc agtgtgccaa
    11221 aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc cctgaatttt
    11281 ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta ggaaatccag
    11341 taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt
    11401 gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga agcgcattcg
    11461 cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt gttgcttcgt
    11521 cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg gctgtctctg
    11581 ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc actgaagcat
    11641 tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta cagacactca
    11701 cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca tgagaaaatc
    11761 ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca gcggccaatt
    11821 ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct acttgtccag
    11881 cgcctatgat tgctactttc ttcatttatt tctccctcct ttgcttattt acttattgtt
    11941 attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca gacagttttt
    12001 caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca aacgacattc
    12061 atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc gagtaaaatc
    12121 aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat gacccacata
    12181 ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca tatgataaaa
    12241 atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac gcgcatccgc
    12301 taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa taaccgtacc
    12361 tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt tatagtctcc
    12421 ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc cccaagctgc
    12481 tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct cagcaatttg
    12541 acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat cgtagccgtt
    12601 acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg tgtcgatatt
    12661 ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt tgataaatct
    12721 aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt
    12781 tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg gtctacataa
    12841 cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa
    12901 tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt acggttccat
    12961 cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt tgcattattg
    13021 aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa ggcaacataa
    13081 gaaatgatcc cgccatagcc gatccctctt aaagaagctt gtaatgcggc agcactgcca
    13141 ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag acgaaaaccg
    13201 acatctgctt tcataggatc tagcgtgtaa tcggcaccgt atttttctgc taattctcta
    13261 cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt agctaattga
    13321 atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc tgctcgaaca
    13381 tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac tgcatttttc
    13441 cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt tgtaatcagc
    13501 gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc tgtcaccaaa
    13561 ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc gccaaatacc
    13621 acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc tgaaaattct
    13681 tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg ttttggagta
    13741 gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc gtaatctttg
    13801 aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt cctctttctt
    13861 ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc gttcagataa
    13921 cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca ttcgtcccca
    13981 aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga
    14041 ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa agcaataaac
    14101 gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa tgatctggat
    14161 ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata
    14221 actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga taatcagtgc
    14281 tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa caacgatcaa
    14341 aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc gagctttgaa
    14401 gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag ttacggaggc
    14461 tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa tctcagcatt
    14521 gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc
    14581 aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt
    14641 gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct ttttattttt
    14701 catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat gatgaacaat
    14761 agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt ctgatacaaa
    14821 taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa catatagaac
    14881 atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc cggcagtgtc
    14941 acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc ataaagcgtt
    15001 ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata gtatctccag
    15061 atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca cagcggtgct
    15121 tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata actattattg
    15181 aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact taataaaatc
    15241 agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc tacggctaat
    15301 cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa acctaaagaa
    15361 cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat aaaaacggcg
    15421 tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga aagcattgga
    15481 atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat ggctaatttt
    15541 aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc tgccaatagc
    15601 tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt tttttgtgcg
    15661 tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac tgcttttccg
    15721 atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc gactagtcca
    15781 aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc aaaagacttg
    15841 atgacttcat tttctttgta accatccagt tcgactactt gtttattcac tggttgagca
    15901 ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa gaaagctgtg
    15961 aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt caatccagat
    16021 actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat attttctgag
    16081 ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat tggtgctgtt
    16141 ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat cgtgtattgg
    16201 gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat cgttacctcg
    16261 ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa cgcttgctca
    16321 ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc tttatctgtg
    16381 aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa accagcttct
    16441 gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg tgcagcaata
    16501 tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata attatcttct
    16561 ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc tttatccatc
    16621 actttagcaa aatcctgact gacttccatt acttctggta atttaccgga acgagctaac
    16681 gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat tttgatgtct
    16741 ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg ttcctgttcg
    16801 acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct ttgactttca
    16861 ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat aatttttttc
    16921 atttttttcc acctcttatt ttattatttt cagccacata tattcatacg gctggaatat
    16981 caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta ggtaatctcc
    17041 ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta agactttctc
    17101 ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta agtaaatcaa
    17161 actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg tcaattcacc
    17221 atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg ctaacaaaaa
    17281 tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt aatcatttcg
    17341 acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta ccaaaaatga
    17401 atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag aacttaacgc
    17461 atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt ttttcttgta
    17521 gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg ttttttccgg
    17581 cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa
    17641 agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact tacttgtatt
    17701 acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga ccatctgtgc
    17761 ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat ttgtctcagt
    17821 gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt ggatgatttt
    17881 atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa atcccacagc
    17941 gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat cgatcatttt
    18001 caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa aagtcgtcca
    18061 tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc tggtccttgg
    18121 tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt
    18181 atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat gattgcagac
    18241 aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc atgtgcccaa
    18301 acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg tatatgggaa
    18361 aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat aaaatttctt
    18421 aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag taatcaagaa
    18481 aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct gataaacact
    18541 gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc
    18601 ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt cccaacgatt
    18661 tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat tttgatatac
    18721 acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta aaaaaatatg
    18781 gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa aaaagaatgt
    18841 ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa
    18901 acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg tctccagagt
    18961 attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc aagcaatcaa
    19021 agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg gttttgacgc
    19081 gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt ttttttcaga
    19141 tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga ttctacaaac
    19201 ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat
    19261 ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg aattggatca
    19321 atacaatatt cctattgtgg taatcggaaa agtcggaaac gactacacga acatctactc
    19381 agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga tcaagaatgg
    19441 acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca atgatcgtct
    19501 caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg aatggattat
    19561 agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa tgaagctacg
    19621 agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta tttacaaagt
    19681 ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat tcagtgatcc
    19741 cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga caaatcagtt
    19801 aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc ctgaagaaaa
    19861 aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac aagaaatgta
    19921 gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg ttcttttttc
    19981 aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt attttccatc
    20041 tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt ccacagggaa
    20101 gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt tttaaacaca
    20161 tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca taaatagaat
    20221 aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata gctagcaaaa
    20281 tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa aatgatctat
    20341 ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct taattggata
    20401 cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa ttattttaga
    20461 caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa aatatctatt
    20521 ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct gaaatactgt
    20581 agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa cgttgcatac
    20641 cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca agaagaggat
    20701 ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg aatgatgcag
    20761 cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag acaggtcgag
    20821 aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta aagtctgcga
    20881 tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca cgaactttac
    20941 gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc ccttgcatac
    21001 ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca tcgcgaactt
    21061 gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg tggatcatat
    21121 cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt gtatctgttt
    21181 ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata tcgatgacat
    21241 cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca aagatttctt
    21301 ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta tggaatgtac
    21361 gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta ccaactgctt
    21421 ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt ttgcagacac
    21481 catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca cctgcatcaa
    21541 cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc aattcatttg
    21601 tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct tctaatgatt
    21661 cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca gtaccgcaat
    21721 cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc aagtaacctg
    21781 aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga gttgagataa
    21841 acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat tccatgattc
    21901 gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag ttggaaatgt
    21961 taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc aaaccttcca
    22021 tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca cgttcataac
    22081 gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca acttgtttat
    22141 gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg atccctactg
    22201 tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca agcatcttag
    22261 aagtttcttt tactttgaat cgtttgaaga cttctgcgat gatgtttcct aagtttttct
    22321 tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca gtaccagctt
    22381 caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt tcattcagat
    22441 aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt gtgatcatcg
    22501 tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg ttcacaccga
    22561 tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg ttttgatctc
    22621 tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag tagttaccta
    22681 gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc aagatgtttt
    22741 gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat ggaacgtgga
    22801 cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt ggatgcaaac
    22861 ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt cggtgaagcg
    22921 taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa acatcccata
    22981 cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa gctagttcgc
    23041 gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt tcttttggaa
    23101 gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga cctgagtagt
    23161 cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc aacatatgag
    23221 aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga cgaccgttat
    23281 cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg atgattccag
    23341 gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca cgacggtaaa
    23401 gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt gggcgtaaat
    23461 ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt cctgaagcgc
    23521 ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt ccttgtgcag
    23581 atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta tcaagtaatt
    23641 gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt tcacgttttt
    23701 cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt cctggttcga
    23761 tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc atgtctaata
    23821 caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca ggagctgcta
    23881 attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc actccgcatc
    23941 gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca cattcccagt
    24001 cttttgtagg accaaaaatc cgctcacaga acaacccttc gcgttcaggt tttaaggtac
    24061 gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg attttttcgg
    24121 gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa aggggttccc
    24181 tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt gcagctcata
    24241 gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca tcgtcttttt
    24301 catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc tgttcggcaa
    24361 attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg tcgcgaagtt
    24421 cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat tgcaattctt
    24481 tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca cccttgacga
    24541 ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc aagatttctt
    24601 gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca ccaaaacgtt
    24661 gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag taaggtccga
    24721 ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg tacatcacac
    24781 cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga acagttttcg
    24841 catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca cttgcaccat
    24901 caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc aagtgtaatt
    24961 ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc atgatatcga
    25021 ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg gatacaaccc
    25081 ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt ttttgaacga
    25141 tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct tcacgagtaa
    25201 agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat gatgtatcgc
    25261 ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct gcagataatt
    25321 ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg acttctgcac
    25381 caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg acattaggga
    25441 tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat tcatattctt
    25501 caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg atgatcgcat
    25561 cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg cctagagcca
    25621 tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg actttctcac
    25681 ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga cggaattttg
    25741 ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca gaagcatcaa
    25801 cgtattcaac gacaccatca tgtttacata acaaagcagc tcctgagtca tgcgctgatt
    25861 tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc actgcttgac
    25921 gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag aaaggaatac
    25981 atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct actttatcaa
    26041 tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga gcaaatgttc
    26101 catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg atatcagctg
    26161 tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga cgataaggtg
    26221 tttcgataaa tccgaattta tttactttcg cataactgga caaactattg atcaacccga
    26281 tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag tagtgaacgt
    26341 cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag gcgaaaagac
    26401 gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt gacaactgag
    26461 aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc aattgttgag
    26521 gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt tccatacgtg
    26581 ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata cgacgatttc
    26641 ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag aaatagctca
    26701 ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga tagccgtttc
    26761 cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg acttggacag
    26821 tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa tttaggccat
    26881 tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt cctttttcaa
    26941 cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga ttcaatagac
    27001 gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct aagtcgtaac
    27061 gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct gtttttggtt
    27121 cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt gagtcgcttg
    27181 cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg ccgaagattt
    27241 caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt aaaggaatct
    27301 tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc atttctagcc
    27361 aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt ttgtcgactt
    27421 ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact cgctctgccc
    27481 cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag aatacttctt
    27541 gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt aatggtgcag
    27601 agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct ttcaattcat
    27661 aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa atgtcttcaa
    27721 acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga atttcgatca
    27781 aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg cgatgttttc
    27841 cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat tcaaaaagac
    27901 tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca acagacattt
    27961 tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa ccttacttcg
    28021 tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt cgtttctgcc
    28081 ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct ataaaagtca
    28141 acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt caattttatg
    28201 ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta tactttagaa
    28261 aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc accaacacaa
    28321 ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga tggatacaaa
    28381 cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag caaaaagcca
    28441 cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta tcaagcattc
    28501 tactttttca atttattttc cgacttttca atccatttgg acattttgtc tgaatggatt
    28561 ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc atcggattag
    28621 gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc gcagaggcat
    28681 cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg ttagatgctg
    28741 cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc gataatctaa
    28801 caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg agtgagaaac
    28861 tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca caatctttat
    28921 taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg gaacaagccc
    28981 atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca gatatggtgc
    29041 caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa acaatcggca
    29101 tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca gaaaaagaat
    29161 tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat gatgtccaac
    29221 aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact gataacacgt
    29281 tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca cctgtcgttg
    29341 ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc gactacgaaa
    29401 gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag aatccaagtg
    29461 aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac atggccaaag
    29521 cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga ggattctcat
    29581 gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat gggccctact
    29641 tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga ctgctgaagg
    29701 aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct atcatccatt
    29761 gacagcttgt cttctaggct ttttagggag tgcggcagca gggcttgttt ccggactgct
    29821 tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga ctggtttata
    29881 ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc agccaacgat
    29941 ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga tcgtcggtct
    30001 gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag aaacaggact
    30061 tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga tcaacacaga
    30121 taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt ctggctcctt
    30181 gcttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga tcgtaattgg
    30241 acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat ttctccgtct
    30301 cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag tcttcgaact
    30361 gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga tctgtctttc
    30421 tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg gtagtgtcaa
    30481 atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg aacgatcaac
    30541 gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt cgtgacgatc
    30601 atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg tacattttct
    30661 gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc tgttgtcgaa
    30721 cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc cgttcgttta
    30781 acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg cttcttccgt
    30841 ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct aaatttgggg
    30901 ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg tcaagctatt
    30961 acgttattga tggccacatt acagcgtcca gaactgatct tattagatga acacactgca
    31021 gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct gatcactgag
    31081 cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg ctatgggagt
    31141 cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga ggaaaaacag
    31201 cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac ggcattaaaa
    31261 gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa atgaacgccc
    31321 accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa ccgttgtttc
    31381 ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa ggagcttgat
    31441 gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga ttttctttcc
    31501 ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa agccatcaaa
    31561 gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg ttacctgccc
    31621 tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat tcctgatttg
    31681 aaagtacaaa cgatgatgac ttctgaatct acgatgaaag atgccaaact ggcagcagct
    31741 caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc tcgtggacgt
    31801 tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct cttgctccat
    31861 ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag tgcctgtgtg
    31921 aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa tgatatctac
    31981 atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat ggaaagcggc
    32041 aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca aaatcatttt
    32101 ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc tagtgtcaca
    32161 aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc tcacccagaa
    32221 aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt gtcgttcaat
    32281 caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg cgaactgatc
    32341 gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct cacatctatc
    32401 cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag tgtccttttt
    32461 tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct ctatcctcta
    32521 gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa tgaatcaatg
    32581 caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc gcttcctttt
    32641 tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg cgacttattt
    32701 tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa cgtctcgcat
    32761 agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga cgcattcctc
    32821 gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa ggttccagcc
    32881 ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc agatcttcta
    32941 tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct tgttcgttat
    33001 ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt ggagaagttc
    33061 ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc agccaagtca
    33121 ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc tcaatagaga
    33181 cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg catagcatat
    33241 ctaaataatc catttatttt cctccttttt atttatccta cttacttttt cctaattcta
    33301 ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca gtcaccatca
    33361 tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga acaagaagca
    33421 acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa acgaacagtt
    33481 taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga aggggaaaaa
    33541 tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc ttttcgtatg
    33601 gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt tataatgatc
    33661 taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt gctaaaaata
    33721 agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt gatgcaaaaa
    33781 ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat tatatacaaa
    33841 aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga atgaataaat
    33901 ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca gtcaatggat
    33961 ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg catgaatata
    34021 ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt gcaacgatca
    34081 ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg acaatcgatt
    34141 taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa gtgttaacga
    34201 tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt aatgtcatca
    34261 acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt tcaaatcatg
    34321 aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag atgactgatt
    34381 tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact atccgcatta
    34441 ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca gctaccaatc
    34501 gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca gacacaagcg
    34561 acagtacaga tacatctacc acaagtgata gctcagattc atcaacttca agcgacagta
    34621 cagatactac ctctaccaca agtgatagca cggatacatc agcttcaagc gacagtacag
    34681 atactacctc taccacaagt gatagcacgg acacatcagc ttcaagtgac agtacagata
    34741 ctacctctac cacaagtgac agcacggaca cgtcagcttc aagcgacagt acagatacta
    34801 cctctaccac aagtgatagc acggacacat cagcttcaag tgacagtaca gatactacct
    34861 ctaccacaag tgatagcacg gacacgtcag cttcaagtga cagtacagat actacctcta
    34921 ccacaagtga tagctcagat tcatctacca ctacaagtga tagcacggac acgtcagctt
    34981 caagtgacag tacagataca tcctctacca caagtgatag ctcagattca tcaacttcaa
    35041 gcgatagtac agacacgtcg tctaccacaa gcgatacacg tcatgcaagt gtctccttct
    35101 caaaaacaag ttcggataac aggacgaact ttaacttaac taataaaaca agcaatgaca
    35161 atggaaacaa atcaaaaaga gcacttccta aaactggaag tcaatcgaat aattggataa
    35221 cactcgcagg cgttatatta cttgtaatcg gtttaagaat cacttttatg agttataaga
    35281 aatcgaaaag gtaaaaaaat attcaag
//
LOCUS       DACDCJ010000020        35008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDCJ010000020 DACDCJ010000000
VERSION     DACDCJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.020"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..807)
                     /locus_tag="IV038_000998"
     CDS             complement(<1..807)
                     /locus_tag="IV038_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8341234.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKK"
     gene            complement(1031..2971)
                     /gene="thrS"
                     /locus_tag="IV038_000999"
     CDS             complement(1031..2971)
                     /gene="thrS"
                     /locus_tag="IV038_000999"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP8341235.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3308..3487)
                     /locus_tag="IV038_001000"
     CDS             complement(3308..3487)
                     /locus_tag="IV038_001000"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP8341236.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3534..5357)
                     /gene="cadA"
                     /locus_tag="IV038_001001"
     CDS             complement(3534..5357)
                     /gene="cadA"
                     /locus_tag="IV038_001001"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP8341237.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5635..7914
                     /locus_tag="IV038_001002"
     CDS             5635..7914
                     /locus_tag="IV038_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8341238.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8007..8654)
                     /locus_tag="IV038_001003"
     CDS             complement(8007..8654)
                     /locus_tag="IV038_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341239.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8833..9552)
                     /locus_tag="IV038_001004"
     CDS             complement(8833..9552)
                     /locus_tag="IV038_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8341240.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9683..10834)
                     /locus_tag="IV038_001005"
     CDS             complement(9683..10834)
                     /locus_tag="IV038_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8341241.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11051..11791)
                     /locus_tag="IV038_001006"
     CDS             complement(11051..11791)
                     /locus_tag="IV038_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8341242.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11795..12118)
                     /gene="rsfS"
                     /locus_tag="IV038_001007"
     CDS             complement(11795..12118)
                     /gene="rsfS"
                     /locus_tag="IV038_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP8341243.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12175..12768)
                     /locus_tag="IV038_001008"
     CDS             complement(12175..12768)
                     /locus_tag="IV038_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP8341244.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12752..13399)
                     /locus_tag="IV038_001009"
     CDS             complement(12752..13399)
                     /locus_tag="IV038_001009"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP8341245.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13412..13723)
                     /gene="yhbY"
                     /locus_tag="IV038_001010"
     CDS             complement(13412..13723)
                     /gene="yhbY"
                     /locus_tag="IV038_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP8341246.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13739..14848)
                     /gene="yqeH"
                     /locus_tag="IV038_001011"
     CDS             complement(13739..14848)
                     /gene="yqeH"
                     /locus_tag="IV038_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP8341247.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14845..15372)
                     /locus_tag="IV038_001012"
     CDS             complement(14845..15372)
                     /locus_tag="IV038_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP8341248.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15552..16337)
                     /locus_tag="IV038_001013"
     CDS             complement(15552..16337)
                     /locus_tag="IV038_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP8341249.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16330..17199)
                     /locus_tag="IV038_001014"
     CDS             complement(16330..17199)
                     /locus_tag="IV038_001014"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP8341250.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17200..18579)
                     /locus_tag="IV038_001015"
     CDS             complement(17200..18579)
                     /locus_tag="IV038_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP8341251.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18587..19006)
                     /gene="fabZ"
                     /locus_tag="IV038_001016"
     CDS             complement(18587..19006)
                     /gene="fabZ"
                     /locus_tag="IV038_001016"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8341252.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19031..19507)
                     /gene="accB"
                     /locus_tag="IV038_001017"
     CDS             complement(19031..19507)
                     /gene="accB"
                     /locus_tag="IV038_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP8341253.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19510..20745)
                     /gene="fabF"
                     /locus_tag="IV038_001018"
     CDS             complement(19510..20745)
                     /gene="fabF"
                     /locus_tag="IV038_001018"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP8341254.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGTKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20764..21501)
                     /gene="fabG"
                     /locus_tag="IV038_001019"
     CDS             complement(20764..21501)
                     /gene="fabG"
                     /locus_tag="IV038_001019"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP8341255.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21513..22430)
                     /gene="fabD"
                     /locus_tag="IV038_001020"
     CDS             complement(21513..22430)
                     /gene="fabD"
                     /locus_tag="IV038_001020"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP8341256.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22537..22761)
                     /locus_tag="IV038_001021"
     CDS             complement(22537..22761)
                     /locus_tag="IV038_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP8341257.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22814..23776)
                     /locus_tag="IV038_001022"
     CDS             complement(22814..23776)
                     /locus_tag="IV038_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP8341258.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23786..24226)
                     /locus_tag="IV038_001023"
     CDS             complement(23786..24226)
                     /locus_tag="IV038_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8341259.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24532..25497
                     /locus_tag="IV038_001024"
     CDS             24532..25497
                     /locus_tag="IV038_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP8341260.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25554..25784)
                     /locus_tag="IV038_001025"
     CDS             complement(25554..25784)
                     /locus_tag="IV038_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP8341261.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25870..26757)
                     /locus_tag="IV038_001026"
     CDS             complement(25870..26757)
                     /locus_tag="IV038_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP8341262.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26846..28069)
                     /locus_tag="IV038_001027"
     CDS             complement(26846..28069)
                     /locus_tag="IV038_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8341263.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28086..29165)
                     /locus_tag="IV038_001028"
     CDS             complement(28086..29165)
                     /locus_tag="IV038_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8341264.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29296..31023)
                     /gene="ptsP"
                     /locus_tag="IV038_001029"
     CDS             complement(29296..31023)
                     /gene="ptsP"
                     /locus_tag="IV038_001029"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP8341265.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31023..31289)
                     /locus_tag="IV038_001030"
     CDS             complement(31023..31289)
                     /locus_tag="IV038_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP8341266.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31768..34002
                     /locus_tag="IV038_001031"
     CDS             31768..34002
                     /locus_tag="IV038_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP8341267.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34150..34470
                     /locus_tag="IV038_001032"
     CDS             34150..34470
                     /locus_tag="IV038_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP8341268.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34675..34899)
                     /locus_tag="IV038_001033"
     CDS             complement(34675..34899)
                     /locus_tag="IV038_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP8341269.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         9858 a         7570 c         6090 g        11490 t
ORIGIN      
        1 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
       61 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
      121 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
      181 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
      241 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
      301 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
      361 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
      421 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
      481 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
      541 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
      601 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
      661 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
      721 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
      781 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
      841 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
      901 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
      961 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     1021 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     1081 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     1141 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     1201 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     1261 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     1321 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     1381 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     1441 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     1501 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     1561 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     1621 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     1681 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     1741 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     1801 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     1861 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     1921 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     1981 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     2041 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     2101 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     2161 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     2221 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     2281 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     2341 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     2401 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     2461 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     2521 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     2581 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     2641 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     2701 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     2761 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     2821 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     2881 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     2941 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     3001 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     3061 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     3121 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     3181 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     3241 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     3301 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     3361 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     3421 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     3481 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     3541 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     3601 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     3661 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     3721 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     3781 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     3841 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     3901 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     3961 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     4021 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     4081 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     4141 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     4201 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     4261 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     4321 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     4381 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     4441 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     4501 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     4561 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     4621 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     4681 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     4741 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     4801 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     4861 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     4921 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     4981 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     5041 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     5101 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     5161 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     5221 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     5281 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     5341 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     5401 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     5461 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     5521 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     5581 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     5641 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     5701 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     5761 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     5821 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     5881 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     5941 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     6001 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     6061 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     6121 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     6181 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     6241 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     6301 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     6361 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     6421 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     6481 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     6541 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     6601 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     6661 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     6721 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     6781 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     6841 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     6901 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     6961 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     7021 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     7081 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     7141 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     7201 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     7261 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     7321 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
     7381 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
     7441 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
     7501 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
     7561 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
     7621 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
     7681 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
     7741 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
     7801 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
     7861 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
     7921 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
     7981 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
     8041 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
     8101 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
     8161 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
     8221 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
     8281 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
     8341 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
     8401 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
     8461 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
     8521 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
     8581 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
     8641 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
     8701 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
     8761 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
     8821 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
     8881 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
     8941 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
     9001 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
     9061 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
     9121 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
     9181 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
     9241 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
     9301 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
     9361 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
     9421 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
     9481 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
     9541 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
     9601 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
     9661 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
     9721 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
     9781 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
     9841 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
     9901 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
     9961 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    10021 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    10081 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    10141 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    10201 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    10261 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    10321 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    10381 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    10441 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    10501 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    10561 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    10621 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    10681 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    10741 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    10801 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    10861 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    10921 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    10981 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    11041 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    11101 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    11161 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    11221 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    11281 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    11341 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    11401 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    11461 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    11521 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    11581 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    11641 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    11701 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    11761 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    11821 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    11881 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    11941 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    12001 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    12061 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    12121 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    12181 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    12241 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    12301 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    12361 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    12421 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    12481 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    12541 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    12601 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    12661 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    12721 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    12781 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    12841 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    12901 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    12961 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    13021 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    13081 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    13141 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    13201 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    13261 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    13321 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    13381 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    13441 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    13501 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    13561 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    13621 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    13681 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    13741 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    13801 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    13861 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    13921 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    13981 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    14041 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    14101 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    14161 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    14221 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    14281 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    14341 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    14401 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    14461 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    14521 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    14581 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    14641 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    14701 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    14761 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    14821 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    14881 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    14941 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    15001 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    15061 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    15121 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    15181 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    15241 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    15301 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    15361 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    15421 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    15481 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    15541 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    15601 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    15661 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    15721 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    15781 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    15841 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    15901 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    15961 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    16021 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    16081 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    16141 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    16201 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    16261 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    16321 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    16381 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    16441 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    16501 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    16561 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    16621 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    16681 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    16741 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    16801 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    16861 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    16921 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    16981 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    17041 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    17101 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    17161 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    17221 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    17281 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    17341 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    17401 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    17461 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    17521 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    17581 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    17641 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    17701 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    17761 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    17821 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    17881 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    17941 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    18001 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    18061 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    18121 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    18181 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    18241 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    18301 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    18361 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    18421 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    18481 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    18541 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    18601 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    18661 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    18721 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    18781 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    18841 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    18901 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    18961 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    19021 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    19081 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    19141 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    19201 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    19261 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    19321 cttgttcaga tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    19381 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    19441 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    19501 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    19561 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    19621 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    19681 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    19741 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    19801 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    19861 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    19921 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    19981 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    20041 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    20101 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    20161 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    20221 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    20281 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    20341 cattggtacg aacattggag aaactcgttt tgtacctttt tcatgtagac gaataacttg
    20401 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    20461 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    20521 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    20581 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    20641 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    20701 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    20761 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    20821 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    20881 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    20941 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    21001 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    21061 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    21121 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    21181 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    21241 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    21301 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    21361 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    21421 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    21481 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    21541 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    21601 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    21661 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    21721 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    21781 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    21841 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    21901 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    21961 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    22021 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggt agcttctgtc
    22081 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    22141 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    22201 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    22261 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    22321 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    22381 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    22441 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    22501 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    22561 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    22621 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    22681 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    22741 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    22801 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    22861 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    22921 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    22981 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    23041 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    23101 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    23161 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    23221 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    23281 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    23341 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    23401 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    23461 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    23521 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    23581 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    23641 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    23701 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    23761 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    23821 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    23881 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    23941 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    24001 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    24061 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    24121 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    24181 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    24241 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    24301 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    24361 aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata
    24421 ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat
    24481 tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa
    24541 gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt
    24601 aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat
    24661 cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat
    24721 ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat
    24781 taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt
    24841 accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt
    24901 ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg
    24961 ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact
    25021 tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg
    25081 acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt
    25141 atgcttcaaa aagatatcga attagttact tattttatta gacggatcca ggaacaaaag
    25201 cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag
    25261 aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac
    25321 gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg
    25381 tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa
    25441 gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg
    25501 cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg
    25561 attaagtcat agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga
    25621 gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc
    25681 tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata
    25741 gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt
    25801 cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa
    25861 actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa
    25921 agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc
    25981 ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa
    26041 atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg
    26101 agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt
    26161 ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt
    26221 acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg
    26281 caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc
    26341 ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac
    26401 cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag
    26461 gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat
    26521 ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag
    26581 aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac
    26641 taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag
    26701 tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt
    26761 ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga
    26821 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
    26881 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
    26941 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
    27001 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
    27061 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
    27121 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
    27181 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
    27241 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
    27301 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
    27361 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
    27421 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
    27481 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
    27541 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
    27601 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
    27661 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
    27721 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
    27781 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
    27841 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
    27901 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
    27961 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
    28021 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
    28081 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
    28141 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
    28201 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
    28261 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
    28321 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
    28381 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
    28441 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
    28501 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
    28561 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc ttttcgttcg
    28621 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
    28681 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
    28741 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
    28801 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
    28861 agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
    28921 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
    28981 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
    29041 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
    29101 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
    29161 aacatagcct acaaactgtc actcaaactt aagccctatt tttgtctatt cttactgaca
    29221 aaaacgtttt cttaaaaaag aacaaaaaaa ctggcgtagc tcttttacaa gccacaccag
    29281 cttttgatac gttgtttatt ttacatattc atgaacaagt tcaacgactt cttccatcat
    29341 atcgcattct ttcaatgcac gatcagcaag ttcagccatt ttcgctgtgt ccaagcgttt
    29401 cattaaacta cgcgttttaa gaatagatgt cgcactcatt gagaactcat ctagacccat
    29461 acctactagc aatggaacag ctgtttgatc tccagccatt tctccacaca tacctgccca
    29521 ttttccttca gcgtgtgctg catcaattac atttttgatc aaacgtaaaa ttgatgggtt
    29581 atatggttgg tacaagtaag aaacgcgttc gttcatacgg tctgctgcca ttgtgtattg
    29641 aatcaaatcg ttcgtaccta cagagaagaa atctacttct tttgcaaact tgtctgctaa
    29701 gactgctgct gctggaatct cgatcatgat gccgacttgg atcgtatcag atacttcctt
    29761 gccttcactt atcaattttt gtttttcttc ttcaaagatc gcttttgctg cgcggaattc
    29821 tttgagtgta gctaccatag ggaacatgat acgtaaatta ccatgaacag atgcacgaag
    29881 caatgcacgc atttgtgtac ggaacatttc gtcgccttgt tcagataaac tgatacgcaa
    29941 tgcacgatat cctaagaatg gattcatttc atgaggtaat tgcagatatg gtagttcttt
    30001 atctccaccg atgtccattg tacgaacaac tacaggtttt ccttccatac cttctaatac
    30061 agctttgtaa gcttcatatt ggtcatcttc tgttgggaag tctggagaat ccatatacag
    30121 gaattcagta cggtacagac caactgcttc tccaccatta ttatggacac caactagatc
    30181 ttttggtgtt ccgatgtttg cagctaattc aaaatgcttg ccgtcagctg tgacagtttc
    30241 tgcatttttt aatttttccc attctgcttt ttgtgctgcg taatctgcac ctgctttttc
    30301 aaattcagct ttttgttcat ctgtaggatc gatgattaca tcgccttcga tcccgttgac
    30361 agccaaaata tcgccttctt tgactttagc agtaatttct tttgtcccaa cgattgctgg
    30421 aatttctaaa gaacgtgcca tgatagcaga atgtgaagta cgtccaccaa tgtctgttac
    30481 aaacgctttt acaaaattgc gatctaattg tgctgtatca cttggagtca agtcatgtgc
    30541 aacaacgacc acttcttcgt tgatcattga tggatttggt aaagtaacgc ctaataagtg
    30601 cgctaaaata cgtttagcaa cgtcacgaat atctgcagca cgctcttgca tataagcatt
    30661 atcttccatt gcttcgaaca taccgatata catatccgtt acttctttta gcgctgattc
    30721 agcatttacg ctgttgtctt taatattttg cttgatttga cctaccattt ctggatctga
    30781 aagaaccatc aaatgggcat caaatacttg cgcttctgct tcacctaagc tttgcgctgc
    30841 tttttcacga atttgctgca attcttcagt agatttagct aatgcagcat ccaaacgagc
    30901 ttcttcagcc gcagtatctt ctactgttgc cttactgaat gttaaatccg gttgaactag
    30961 caggtaagct tttgcaacgg ctacaccgtc actagcggca atccctttta gcatttcaac
    31021 cattattcag ataagccttc tttcttcatt gtttcaacga ttgctgccat tgcatcagct
    31081 tcgtcagcac cttctgcagt aatagtaaca tcagaacctt gacctacacc taaagacata
    31141 acgcccatga ttgatttcaa gtttactgat ttacctttat actctaagtt tacatcagag
    31201 ttaaatttgc ttgctgtttg tactaatagt gtagctggac gagcgtggat cccagtttct
    31261 gctactacgt gaaattcttt cttttccata ttaatcggtc tcctttaagt gagtgttgtt
    31321 tttatgtgag ggttttcttt tttagtaatt cagagaatcc taacaaatgc caccctttca
    31381 tctgtaaaga ttaacatttt ttttcgttag atacaacagt tttcattcaa tttcagataa
    31441 aagtattcaa attattccgg attctttttc atccttttct tcacatttta taacaaacgt
    31501 gtttatttcc tttttcttgt cgctttctct tctcctgacg tttatcattc tttcctttga
    31561 ccctctttgt tttcatatga aacgaattac ttgcttccct ttttattttt agtataataa
    31621 gagtcaaagg tcaggaatgg tcaatagaga tttcaccttt tctacctcat aaatctctta
    31681 aaaaactagg ggttattctt ttacctcatg aaaaacgaga tgtaaaatat ggatgtaccc
    31741 atttttgtga aaggacgtga atgattcatg ctttgtcaaa actgcggcaa gaatgaggca
    31801 acgattcact tgtatgccac tgtcaatggt cagcggacac aactcgatta ctgtcagagc
    31861 tgttatcaaa aaatcaaaaa tcaacaaaat ggaggtctga ctaatatggc ccaaaatgat
    31921 ccatttggat ttggcagttt ggacgatctt ttccgttcca tgtctcgcca aatgcaacag
    31981 caaggaaatt atgagcaaac accaccaaca caatttggcg gaaacaacaa cttcaatggt
    32041 ggtcagccac ctcaaggcaa tacagaaggt ttattgggag aatacggaat caatatcaca
    32101 gaacaagcac gacaaggtga tatcgatcct gtgatcggac gcgatgacga aatcaaacga
    32161 gtcattgaaa ttttaaatcg ccgaaccaag aacaatcctg tcttgattgg agaaccaggt
    32221 gttggtaaaa cagcagttgt tgaaggttta gcacaaaaaa tcgtagatgg tgatgtaccg
    32281 caaaaattaa tggacaaaga agtcattcgt ttagatgtcg tatctttagt ccaaggtact
    32341 ggtatacgag gacaatttga agaacgtatg caaaaattga ttgaagagat ccgtcaagca
    32401 gaaaatgtga tcctattcat agatgaagtt cacgaaatcg tcggtgctgg ttctgctggt
    32461 gatggaaata tggatgctgg taatatttta aaaccagctt tagctcgagg agaattgcaa
    32521 atggttggtg ctactacctt aaatgagtac cgcatcatcg aaaaagacgc tgctttagaa
    32581 cgtcgtatgc aacctgtacg ggtagatgaa ccaactgtcg aagaaacgat cagtattttg
    32641 aaaggtttgc aaaaacgtta tgaagactat catcatgtaa aatatactga tgaggccatc
    32701 gaagcagccg caactttatc caatcgttac gtccaagatc gcttcttgcc ggataaagcc
    32761 atcgatttgc tagatgaaac aggttctaag aaaaacttga cgatccaaat cgttgatcct
    32821 aaaacaatcg agaaaaaact gcaagaagca gaagaacaaa aagttttagc ttctcgagaa
    32881 gaagatttcg aaaaagcagc ttattatcgc gatcagatca ataaacttca aaaaatgaaa
    32941 gaacgtcagc tcactgaaga ggaaacacct gtgattaccg aaaaagatat ggaaaaaatc
    33001 gttgaacaac ggacaggtat tccagtgggt gaattaaaag aaaaagaaca gacacaactg
    33061 aaaaacttag cagatgactt gaaagctcat gtcatcggac aagataacgc tgtagatcga
    33121 gtagcgaaag cgattcgccg aaaccgtgtt ggattaaaca aacagaatcg tccaatcggc
    33181 tccttcttat ttgttggacc aactggtgta ggtaaaacag agcttgcgaa acaacttgcc
    33241 tacgaactat tcggttcaca agattcaatg attcgtttcg atatgtctga atatatggaa
    33301 aaacatagtg tctctaaatt gattggttct cctccaggtt atgtcggata cgaagaagct
    33361 ggtcaattaa cagaaaaagt acggagaaat ccttacagtc ttgtcttgtt agatgaagta
    33421 gaaaaagctc atccagatgt tttgcatatg ttcttgcaaa tcttagatga tggtcgattg
    33481 acagatgcac aaggaagaac ggtcagcttt aaagatacga tcatcattat gacaagtaat
    33541 gccggaactg gtaaagtaga agcaaacgtt ggttttggtg cggcacgcga aggtgttact
    33601 cgttctgtat tgaaccaatt aaacaactac tttacaccag aatttctgaa ccgttttgac
    33661 gggatcatcg aattcagtgc attatcaaaa gaaaacttga tgacaatcgt cacactaatg
    33721 ctagacgatg tcaaccaaat gctggcagca cagcaacttc acatcgaagt tcctacgaat
    33781 gtcaaagaaa aattagttga cttaggctat gatccatcta tgggtgctcg tcctcttcga
    33841 cgcacgatcc aagaacaaat cgaagatggt atcgctgaat tttatcttga tcacccttct
    33901 atccatgagt tgaaagcaaa actggataaa gatggaaaaa tcatcgttac ttctaaacca
    33961 gaacgtttgg ctaaagaatc agccgaagaa acggctgaat aaatccagtt ttatagcgtg
    34021 agacggtatt tctttgagaa atgccgtctc attctttttt cagaaaagtc aaaatactgt
    34081 tcattttata aaaaagaaat tataataaga gaaacaagca attaaaaata cataaaagag
    34141 gtgagcgcca tgaaattggt aaatgtaaca aacagccatt cccgtcttgt attaaaccag
    34201 cttgaaaaca ctgatgccca tctagtcaag gtctacacag ccggaaatac cactattgtc
    34261 tacactgaag cccctgaaca taatgaaatt ctgctgatca atgacaaacg taaaatccag
    34321 ccgaaagaaa tcgaagatgc taaaaattat ttcttagata aaatcaaaga cgccgtttac
    34381 cacgaggatg aaatcaaagt aatcgaattg gcaggccttg ttgaaatctc gattccaaaa
    34441 catgaattag gcgctacatt agccgtgtaa acaaattttg aacataataa ttaaagagcg
    34501 tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcgaaagc
    34561 agaagcaact ccttcggaaa taagctgaaa ttcacgaaaa ttgcttttca aatttttgtg
    34621 aattccagct tatttcttgg agttaaacgc ttctgtctca gcctcttttt ctttctagct
    34681 ttttgatttt tctcttttct tttgtacttt tctcttttca ttttcagttc tacgcgtgtc
    34741 tttattttta actttttcct ctttatggat gattgatcgt actttttgtg caatttgctc
    34801 gtttttgtcc cagtctttta catctaataa tcgacataat cgtaacatct cttctttttt
    34861 ctgttttttt ctttcacgtc tttgccattc tgtttccatg tgtcacactc tcctttcttt
    34921 aatcatatgt acgcaaaata atttttatat ttttttgatt agacatttct atgattttaa
    34981 tatagtaaaa agagccgtca cggaaaat
//
LOCUS       DACDCJ010000021        34899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDCJ010000021 DACDCJ010000000
VERSION     DACDCJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.021"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            66..1406
                     /locus_tag="IV038_001034"
     CDS             66..1406
                     /locus_tag="IV038_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8341270.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1601..2044
                     /locus_tag="IV038_001035"
     CDS             1601..2044
                     /locus_tag="IV038_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP8341271.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2306..3724)
                     /gene="nhaC"
                     /locus_tag="IV038_001036"
     CDS             complement(2306..3724)
                     /gene="nhaC"
                     /locus_tag="IV038_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP8341272.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4128..5351)
                     /locus_tag="IV038_001037"
     CDS             complement(4128..5351)
                     /locus_tag="IV038_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP8341273.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5383..6222)
                     /locus_tag="IV038_001038"
     CDS             complement(5383..6222)
                     /locus_tag="IV038_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP8341274.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6239..6919)
                     /locus_tag="IV038_001039"
     CDS             complement(6239..6919)
                     /locus_tag="IV038_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341275.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6921..7994)
                     /locus_tag="IV038_001040"
     CDS             complement(6921..7994)
                     /locus_tag="IV038_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8341276.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(8644..9315)
                     /locus_tag="IV038_001041"
     CDS             complement(8644..9315)
                     /locus_tag="IV038_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8341277.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9403..12081)
                     /gene="mgtA"
                     /locus_tag="IV038_001042"
     CDS             complement(9403..12081)
                     /gene="mgtA"
                     /locus_tag="IV038_001042"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP8341278.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            12741..13178
                     /gene="ndk"
                     /locus_tag="IV038_001043"
     CDS             12741..13178
                     /gene="ndk"
                     /locus_tag="IV038_001043"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP8341279.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13200..14066)
                     /locus_tag="IV038_001044"
     CDS             complement(13200..14066)
                     /locus_tag="IV038_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8341280.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14300..16378)
                     /locus_tag="IV038_001045"
     CDS             complement(14300..16378)
                     /locus_tag="IV038_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8341281.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            16871..18088
                     /locus_tag="IV038_001046"
     CDS             16871..18088
                     /locus_tag="IV038_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8341282.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(18547..19170)
                     /locus_tag="IV038_001047"
     CDS             complement(18547..19170)
                     /locus_tag="IV038_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341283.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(19326..20723)
                     /locus_tag="IV038_001048"
     CDS             complement(19326..20723)
                     /locus_tag="IV038_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8341284.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(20745..21716)
                     /locus_tag="IV038_001049"
     CDS             complement(20745..21716)
                     /locus_tag="IV038_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8341285.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(21723..24023)
                     /locus_tag="IV038_001050"
     CDS             complement(21723..24023)
                     /locus_tag="IV038_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP8341286.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(24025..25350)
                     /locus_tag="IV038_001051"
     CDS             complement(24025..25350)
                     /locus_tag="IV038_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8341287.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            25581..26006
                     /locus_tag="IV038_001052"
     CDS             25581..26006
                     /locus_tag="IV038_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341288.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(26504..27205)
                     /locus_tag="IV038_001053"
     CDS             complement(26504..27205)
                     /locus_tag="IV038_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8341289.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(27221..27661)
                     /locus_tag="IV038_001054"
     CDS             complement(27221..27661)
                     /locus_tag="IV038_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP8341290.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(27661..28236)
                     /locus_tag="IV038_001055"
     CDS             complement(27661..28236)
                     /locus_tag="IV038_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP8341291.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(28647..29153)
                     /locus_tag="IV038_001056"
     CDS             complement(28647..29153)
                     /locus_tag="IV038_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP8341292.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(29164..29643)
                     /locus_tag="IV038_001057"
                     /pseudo
     CDS             complement(29164..29643)
                     /locus_tag="IV038_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(29815..30516)
                     /locus_tag="IV038_001058"
     CDS             complement(29815..30516)
                     /locus_tag="IV038_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8341293.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(30624..32066)
                     /locus_tag="IV038_001059"
     CDS             complement(30624..32066)
                     /locus_tag="IV038_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8341294.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(32080..33945)
                     /locus_tag="IV038_001060"
     CDS             complement(32080..33945)
                     /locus_tag="IV038_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8341295.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIETIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(34066..>34899)
                     /locus_tag="IV038_001061"
     CDS             complement(34066..>34899)
                     /locus_tag="IV038_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8341296.1"
                     /translation="LNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAVQKEFVL
                     RDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLHYAITRA
                     KENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHFVNSQQG
                     NEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLKGEGSDE
                     QDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVSEKKSIQ
                     S"
BASE COUNT        10099 a         7459 c         5985 g        11356 t
ORIGIN      
        1 atttattctt actatttatt cttactatgc attttgattg tgaatttaaa agaaagaggt
       61 tatttatgga agataaaaaa ttgaaaaaac aacttagctc ccgacatatc accatgctag
      121 ctttaggtgg agcgattggt gcaggtctat ttaaaggaag tggcgaagcg attggaatcg
      181 ccggcccttc tgtattgatc gcttttttga ttggcggcat aattttatat atcgtgatga
      241 aaggtctggg aaaaattgtt ttaagtggtg gtgatacgca ccacggatta tctggattga
      301 ttcgtccata tctaggtgct catgctgccg attttaccga ttgggtctac tggtccatgt
      361 ggattatcaa tatcattgct gaagctgttg cagctgcttc ctttttacag ttgtggttcc
      421 cccacatccc agcttgggta tttgttttta tgttggctgt tttaactact atcatcaatc
      481 tttattcggt acgattattc gcagagacag aatattggct ggcctttgct aaaatcagtg
      541 tcattatcct attgatcatt tttgctgctt atcttgtcgg tcagcaaatg ttgggtactg
      601 gtgttttccc tacccttcaa caattaactg atcatggtgg ttttactccc cacggtatga
      661 agggaatcat cagttctttg cttgtagtta tctattctta cggcggttct gagttgattg
      721 ctatcacagt aagcgaagca gatgatccta aacgtgcgat tcctaaagca atcaaaggcg
      781 tcatgggaag aatcatttcc ttttacatta ttcctttatt tttgcttttg attatttttc
      841 cttggaatac tttagcaggc acgaacgtta gtccttttgt gatggtattt gaaaaaatga
      901 atattccatt tgcggcagat atcgtcaatt tcgtgattgt tcttgctctg ttctcgtcta
      961 tcaattcagg tgtatatgct tcttcgagaa ttttgttctt ccgcttgaaa gatcggaaag
     1021 gttcctctag aaaactagct gttttaaaca agcatcaagt accgcaacgt gctgtgctgt
     1081 tttgtaccag tgttctttat ctaggtgtgg ctctttctta ttttgtcggt gacaaattat
     1141 ttggctatct ggcaggttct ctttcctata cagtattatt gatctggatc attatcagtg
     1201 ctgcgtcctt tgtactgaca ctaaggaaag gaacgttatg ggacaaagga atcagctttt
     1261 ttgcattagc agtcttatgg ttgattttcc ttggtatcct attcacgaac tccattgggg
     1321 taactgtatt gaccggtttg ctttacctgt tcatttattt cagttatcaa aaaaagaatg
     1381 atgctttcgt cttgacgaat gaatgaaacc aatagagaat tttattctat ttatattgaa
     1441 tatctggagc tattgaaaaa aaggagctgc gacaaaaatt gttgcagttc ttttttccga
     1501 ataaacggtg ttcaaaaacc atctgtgcag attaattgat tgttctccac gctcatgaca
     1561 tgatagaaga aaggaatgaa gaaaaaggag tttgttttaa atgaaaaagt attggaagtt
     1621 tttgttgatg attggtgttt cgacggtagt catgtttggt atgatgtatt tgaatgttta
     1681 tgcgcttgat catatcttct tcagtcgcac acgtgtattc atggcgttga tgatgggtgc
     1741 agtcatggca gtcatcatgt tgttgttcat gtggaaaatg tatgaaaaca aaaagataaa
     1801 tgctatgatt ttagccgcta gtcttcttgt ttttgccggt tccttattta tggtacgaag
     1861 ccaaacaact gtcaacgata cggcgtggat gaaggcgatg atccctcatc actctattgc
     1921 tatcttgaca agtaaacgag ctgatttaaa agatcctgaa gtaaaaaaat tagcggaaga
     1981 aatcattcag gcacaagaag aagaaatcga caaaatgaaa aaaatgatca aagaaatgga
     2041 atgaccatta tccatccaaa taaaaaaaga ggatgttcca gaagtgatca acttcaagaa
     2101 ataagaagaa gctacttctg gaacaccgtg aatcagaggt tgtgaaaaag ctgccctgca
     2161 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcgttt ggttaggctt
     2221 attaccgcaa atgcaagctt ttgaacaccg tttattcgta aaagggagtg tgacaaaagt
     2281 cttgtcacac tcccttttta cgtttttatt gttctaattg cgctgtcttg cttactgctt
     2341 cttgcggttt cacaaatttc tttgagatcc cccaacctgt aaaggcactg accatcgaaa
     2401 acacaggtga aagcaaactg aaaaacacga atggcaagta agtgagtgta ggaatgccaa
     2461 atgtacttgc tacgaaactt ccggcgattc cccaaggcac taagtagttg attactgttc
     2521 caccatcttc tagtgttctc cctaatacag ttggatcaaa tcctgctttt tcgtaagttt
     2581 ctttaaatgc attaccaggc aggataacag atagaaattg ttctccaata aagatattta
     2641 ctccgatgct tgtcatcaat gttgtaaaaa tcaattttgg ggtgttcgtc atctttcttg
     2701 atactcgttc cataacggtt gagatcaacc caaattcgat caacaaacca cctaatgaca
     2761 atgttagaat aatcaatgac acagttggca tcatgctttc gattcctcca cggctaagta
     2821 gtgaatcgat ttgttggttt ccagtatttg aaacaaatcc cgattcgatg acccctgcca
     2881 acttcgttaa ggaagtttgt gggttttgaa taaagatcat tcctactgct acagcaatat
     2941 tcaacaaaat cgtcaaaatt gccggtacct ttttccatgc acacaagagc attaaaaata
     3001 gaggaatcag tgaccagatc gaaatagtaa agtttgcttc taaaatatca gtcacttcac
     3061 gaatagatga aagacttgcg gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag
     3121 aaacaacaaa tgccggaaca gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg
     3181 catcaacaat agctgctgct aaatttgttg attcggataa tggagacatc ttgtctccaa
     3241 aaacggcacc tgaaacaatg gctccaacaa caagggctgg atttagtcct agtgttgtcc
     3301 caataccgaa aaatgcaatc ccaatcgttg acataacagt aaaggcactc cctacagcgc
     3361 tacctacgat tgcacaaaca acaaatacag aagggacgaa ccactggaca ctaatcaatt
     3421 taaatccaat gaccatgagg gttggaataa ttcctgcttg tatccataca gcaatcaatg
     3481 aaccgactaa aataaaaata aagattggaa tgatccccgg tttgatgcca ttgatgatcc
     3541 cttcgtgcag ccaatctgtc gggtattttt ttatcactgc gtacaacatg accattccga
     3601 tagctgccaa aatcgtgata ttaggtgaaa cctttaatcc gacaacgcca atcgcaatgc
     3661 ctaaaacaat gaccatcaat acaagtgttg cttctaaaaa acttggtgtt cctttttttt
     3721 ccatgataat ctctcctttt ttagttgttc ttatgttcta aaaaagttaa gatatcggat
     3781 tactccgcca caattcatcc gcgattcctc ctttataaaa ataaaaagac ctgctttcaa
     3841 ctaaggacga atgactccgc ggtaccacct taattaaaaa cagatctgtt tttcactttc
     3901 aaacgattat tcaaatgtat ggactttcca tgtaattcgt caattaggct tgatcggttc
     3961 acagcgacca ccgactttct gaacaaaaca cctaaccact actgcgtgaa aagttgactt
     4021 gtatgaagtt taccttccat aagatcattt gtcaatgaaa aagaaaatat tttttcattt
     4081 ttctgaaaat tatttttata actttcgcgc acaactttat ctttttacta gaaagtagcg
     4141 ccctcttgtt tttttcttgc tactatttct tgatgttctt gtttgatttt ttctaatagc
     4201 ttgggttctc tcaataaatc tagagctgta ttggcaagta attccgctgc aatcgcaata
     4261 gaagcaagtc ctttttcact tttagcagca gctttgaatt cttctgtatg tccggcaata
     4321 tatgtctcag agattgctac agtaggctgt atcgttggaa taacttggct aacatttcct
     4381 acatcagaag aacctagact tgttctttgt tccgtttcta tttcttcatc aggaacccct
     4441 gcttcttttg catgttggaa gaataagtca tcgaaagaag gcgttacgat gacatcatcg
     4501 actgcatttt ggaacaatcc aaactcaaaa gtcgtacccg tgcttaatgc agcacctcgt
     4561 acgatatttt ccactttttc atatactttg tctaatgttt gtcgatttcc tgcacgtaaa
     4621 tagaaacgtc cagcagcgta gtctggtatg acattggccg ccacgcctcc atctgtaatg
     4681 attccatgga tattgacatc ttttggcaat tgcaagcgta gagcgttgat tccgttaaac
     4741 acttggatca aggcttccaa agcatttact cctttttcag gtgctgctgc tgcatgagaa
     4801 gcgacaccgt aaaatttgat atcgactgga tcatttgcta aagattctgt cgttttccca
     4861 taacgaaaag ctggatggac acataatgct gcatcgacat cctcaaaaaa accttcccga
     4921 acgaagcttc ccttagctga tccattttcg cctccttctt cccccggagt accatacaca
     4981 cgaatttctc cgccaatctc atcaatgaat tgtttcacta tgctggctgc taatacggag
     5041 taagtaccaa ataaattatg tccgcatgca tgaccaatac ctggtaaagc atcaaattct
     5101 gccaaaaagg caattgtcgg acctggttta ttggaaatat aacgtgcatc aaagcctgtt
     5161 cggtgcccag ctacttcttt ttctacccag aacccttctt tttccaattg tttgattaaa
     5221 gctgctgagg aataaaattc ataattactt acttcaggat ggtcgtggat agctagtgca
     5281 agtgcttgat attctggtaa acggtattga atcagatcct tcaattgatt tgttgagagt
     5341 gtggttgtca taaaaatttc ctcctatgct agtcgttttt tcttattttc tcccgaatgt
     5401 ttcccacgca ggtacgcttg aaccttttga tgttttttcg attactttgg ctgtttcttc
     5461 tgtttgatag gcatctacta ttttttgata cgttttattg tcttcatctt cttttcttgc
     5521 aacgatgata ttgacataag gacgtgcatg ctcatcgact ggttctaaga agattgcatc
     5581 agaagcggga ttaaatccag catctacagc tacaccgcta ttgatgactg aaatatctac
     5641 atctgacaaa gcacgtgctg tttgggaagc atctaactca gaaatgtcta gatttttttt
     5701 attctcggta atatcagaaa cggtaggcgc ttggcctttt gccttgtcca ctttgatcaa
     5761 tccagcagtt tgcaataaca gaagagctct gccgccattc gtgacatcat tagggattgc
     5821 aacttttgct ccatctttga tatcagccac atcttttaac gtattggaat aaattcccaa
     5881 cggtgcatta accgtattcc caatcgatac taaatcagtt ccgtgttctt cattgtaatt
     5941 gtctaaaaag aattgatgtt ggaaggaatt caaatcgatc gatccatctg ctaaagccgc
     6001 atttggctga ttgtaatctg taaatttcac atattccaaa tggatgtttt cctttttcaa
     6061 acggtctttc acatcgtccc atacatctgt atcttcccca atgatcccta gttttacggt
     6121 cgtttctttt ttttcagttg tagctgcttt tccttggctg catcctgcca accaaataat
     6181 tgctacaaat gtaatgactg caaaacgaat aatttttttc attttttctc ctccattttc
     6241 aatgtgtgac ttttttaatc agttgattac tgataaattg actcaaaaaa actaagacca
     6301 gaatcaataa tgtagcgaca atcgttacat ctgtttggaa tcgattatat cctctcgata
     6361 tagcaagatt ccctaatcct ccggcaccta ctgctccagc catggtagtc agtccgatca
     6421 cattgatgat cgtcaacgct gagacccgaa tgataccagg taacccttct aacaaataaa
     6481 cacgtaaaat gatgccgatt gggctcgtgc ccatcgcttc tgctgcttcg atcaccccag
     6541 gatcaacttc tagcagtgca ttctcgattt gacgggcaaa aaacgggaca acgccaaaaa
     6601 tcaaagggac taaagctgct ttggcaccaa tcgtcgttcc aactaagaaa cgagtagtca
     6661 aagctaaaag agaaagcaag ataatgaaag ggatggaacg aatgatattg atcaacttat
     6721 caagtatttc gaacagcagc ctgttagcta gtaatccatt tggacgtgtc actactaacg
     6781 tcacacctaa gatgattccg atgatcccac caatgaatgc cgtccaaaaa gacatatata
     6841 gtgtttgctt tgtcgcttcg atgaattcat ccggaatcaa atagacattc ggcaaatatg
     6901 ttttaaaaaa ttcagtcatt ttagtttcct ccttcgatga ctttgatacg gttatctaat
     6961 tcattttttt gtaaaatagt tacttttaca ccagatttaa tcaaataatc caatgctttt
     7021 tgccgctgtt tggtttctcc agctaaagta acgatcaaac ttcctaaggg tgtttcttgt
     7081 aatagctcta cattaccgta taagatattc gctgttactt ggtacttgct gtaaagatga
     7141 gcgattagag gttcgttcgt ctggttgccg atataactta attccaccaa ccatttattt
     7201 gcaatctgat cggcaaaagc agcatgggca ctgattgttt ccaatgcttg gtcaaggtgt
     7261 gtcgctgtcc gaataaaatc tttagtcaat tcttcttcag gatgactgaa aatctggatg
     7321 ctgctacctt tttcaatgat tttccccgct tccataaccg ctaccttgtt gcagatttct
     7381 ttgacaactt gcatctcatg agtgatcagg acgattgtta gacctagctg acgatttagt
     7441 ttttttaaca gtgcaaggat ctgaagggtc gttttcggat caagtgcact agttgcttca
     7501 tcgcataaca acactttagg ttcatttgcc agagctcgtg cgattgccac tcgttgtttt
     7561 tgcccccccg acagttgttt aggatacgca gatgcttttt cttccagacc gacaagttcc
     7621 agtagttcat tcactttttg acgacgttcc tgttttgatt ttccagaata ctttagagaa
     7681 aaatctacgt tgtcaaaaat cgttcgactt tccattagat tgaaatgctg aaagatcata
     7741 ccgatggcct ttctttcttt tcgcaattct tttggagaga gctggcttaa attcgtctga
     7801 ttgacgatga cttctcctgc tgttggtttc tgcaacaaat tcatcacgcg gactaacgta
     7861 ctctttcctg cacctgagta accgacgatc ccataaacat cccctttttc aatcgttaaa
     7921 tctacagaat ctactgcctg tatctctttt tctttttgtt tgaaacatac agaaatatct
     7981 cgcaattcaa tcattaacat tcctccttgt ctataaaaaa agcatcctca agaaaatgaa
     8041 aatagtcatt ttcttgagga cgcctttttg cgtgttacca cctcatgttc aagctgtgtt
     8101 cacacacaac tcctcttcaa gtaccatcat actctagcaa gatatcggtc gcagccgtca
     8161 ccgccttaca tctatgctcg ggatgaattg ctcgatggcc attttccaat agtcgttcct
     8221 tgcttcttct cagctaccga agctctctgt caaaggttct tctatcgtac ttaccatttc
     8281 aatgcaaata atattcaaaa aaaaaatcct tttactaaca taaactatgt cagtaaaagg
     8341 acgacgtatc gtggtaccac cttttttcgg aagtttcctt ccctcaagcc caacatctca
     8401 aaaagagaga ttggcgttcg ttttaacggt cgaacatttc cgaatttgcc tacttatcga
     8461 caaattattc tccaaggcca ttttcagctt ttcttacttg actcttcaca ccagccagag
     8521 cctctctgca aagtttccaa gcttactttc cttctcaacg aaatcaatat tttgttgttg
     8581 ggaataatct acaggaggaa aactgaaaag tcaagcatga aatccaaatt attttttcta
     8641 tatttatttt tctgtttttt tatctaacat ttcaataaca gttcgaagat cttttccttc
     8701 cgcaagaagg ttcaattttt ccttctcttc gacgctataa tttcgaacaa aactggaatc
     8761 gggaatcaaa acaacgccta gtcctgcacg tgaagcggct gtcgctccta ctaaagaatc
     8821 ttccactgca aaagctgaaa gagcttctaa tcctgttcgt tccaaagctg tcagataagg
     8881 gactggtgaa ggcttgttct ccgatacttc atctccgaac gtaaacgtat caaagtaatc
     8941 ggctaattga aagtgatcca agatatctgt tgctcttttc gtaacagttg tcgtagcaat
     9001 ccctagtttc atatgacgtt cttttaagtc tgtcaatatt tcttttgcat agggtttcaa
     9061 ttggatagct ccacttgtca gctgttgata aaaatattct tcacgctttg ctcggacttc
     9121 ctgcactttt tcaggactgc ctgctgcctg cactaaaata tcaaaacttt gtttccagtt
     9181 ttttccgccc caagcgtcaa cgatttcttt cgggatatga atatcaaatt gctggaatcc
     9241 ccaaagccaa ccatcccgat acgttctttc cgtatccact aacaaaccat ctaaatcaaa
     9301 tattgcacct ttcatctata cattaatcct ttccaaaaca atacacctag tttagcatga
     9361 ctgatccaaa atgaacagaa gcatagagcc cagccgcatc acttataaga gttcttggaa
     9421 tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga cgactgttat
     9481 aaacaaccat aaccagtaat tgccagggag cggcatcaag cctaactcag caccaaatcc
     9541 tgtgaatgga aggactgaac cgacaatgat tcctaatgta gtgataacca gcatggcaaa
     9601 tgttgcattg ctttggataa atggtatttt cggtgtacgc aatgcatgga gtactaatgt
     9661 ttgtgtccac agggattcga caaaccatcc tgcatggaat aatgcgataa aaatcacttg
     9721 ttgtgagcta tccaatgtat ggaaacttcc accaactaca gcaggacaaa taacaaaata
     9781 catcaacaag taagtagtga tatcaaaaat cgaacttgtt ggaccaaaat aaaccatgaa
     9841 ttttccaata ctagatgcat tccatttctt tggctcatcc aaatattctt tatccatccg
     9901 atcccatggt aaggaaatgc aagaaacgtc atagatcaga ttcaaaaata gaagttgcaa
     9961 aggcaacatt ggtaaaaatg gtaagaacgt actggcaatc agtacggaaa acatattgcc
    10021 aaaattagaa cttgctgttg ccttgatata tttcatgatg tttccaaaag tttctcttcc
    10081 agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgctga
    10141 ttcttttgcg atatccacag ctgtatccac ggaaataccg atatcagcca ctttcattgc
    10201 tggagcatca ttgataccat ctcctaaaaa tccgacagta tgcccatttt tacgtaaagc
    10261 tgtcgtcaat cgtgcttttt gctgaggatt caattttacg aatacttcgt gttcttcagc
    10321 tacttgcgct aattcatgat cattcatttc cgagattttc tcaccagtaa tcaattcatt
    10381 gatttctagg cctacttgac ggcacacaga ccgtgtgact aattcattgt ccccagtcag
    10441 aacttttaca gctacaccat gttcttttaa agctttcaac gcttgttttg ttgtttcttt
    10501 tggtggatca aggaaagcaa gataaccaat cagaaccatg tctgactcat ctttgactga
    10561 aaattctcca acaacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccgtc
    10621 ttcattaagt gattgtactt ttgcaagcac cgcttctttt cgttcttttg ttaatggtgc
    10681 gacagtacca ccaagatcaa taaatttaga aatgttcaac atttcttcaa tcgcgccttt
    10741 tgtgatcatc tgtgttttgc cagccggatc agcgactaca acactcatac ggcgacgttc
    10801 aaaatcaaaa ggaatttcat ctactttttg ataattgatt gtatctgtat ctaaagtttc
    10861 tgctgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct
    10921 gtttagataa gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata
    10981 ttcaagaatg attttgtctt gtgtcaatgt tcccgtttta tccgtacata acacatcgat
    11041 cgcaccaaaa ttctggatag catttaaatt tttgatgact gttccttttt tggccatcac
    11101 acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gcgttaaacc
    11161 aactgccaca gataatccaa aaagaaaagc ttctaaccaa tctccttttg taagcccatt
    11221 gataacgata actgctggag ccatcaatga cataaatttg atcagcagca tggatgtttt
    11281 gtggatgcct aattcaaaac ttgattggat gggtttttca gatagcgttt ctgctacatg
    11341 cccaaatagt gtgtcatttc cgacagaaac aatgattcct tccgcgcttc cactgatgac
    11401 attgcttccc ataaagacaa gattttcata acttgtttca ctttgctgat cgtcttcaca
    11461 tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt gagaaatgaa
    11521 tagatccttt gcggacaata aacgcatatc cgctggtatc atatctcctg ctgaaagccg
    11581 aacaagatct ccacagacga tttcttccat aggaatctct gtatactcgc catttcgttt
    11641 gacagctgcc gttactttca caagtgattt cagtttttca gctgcttgat tagaacgcac
    11701 agactggatc aacgtcatcg tcccactgac gataaccata gtgaagataa tggctacacc
    11761 aaagagatct ttatctcctg gagcggctaa tacgtattcc gtaatgaatg aaataattcc
    11821 taatgcaatc aacactaatg taaagggtgt gatgtatgct ttcaatattt ctataatgaa
    11881 tggtgttttt ttgccatatg aaatcgcatt ttcaccataa gtttctcttg aacgctcgat
    11941 ttgttcttct gacaatccat ttggtgatgc atggaacaat tcaaataatt cttgcttcgt
    12001 ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca tctcgatctc
    12061 tcctttgtgt ccaaaagaca gctgttctcg ctgtttttgg tcttttgcta ttcatcggat
    12121 cgatagagaa aatgctgaaa ggggctgaga acagatagat aatagccctg gcctttcctc
    12181 taaatccttg tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat
    12241 tttaattcat gacttccaat catttttcga atagttattt tcccaactcg ctcacctccc
    12301 tttttgtttt ttcacctatg ggatttgcgg caacaaaaaa acctctcgcc aaatactcag
    12361 cgaaaggttt gaaaaagcaa atcaaaaaaa gcttctcaga tctcatcgtt gagttttgac
    12421 actgtacaac gtaaaaagca gatatctgct tttagctacc ttatgaaaag ccttatctcg
    12481 acaattcctg ttctacccat tggcgtctct cgacgttttt gggcagtagc ctatgtttgt
    12541 ataggagcct cacctaacaa atcctattcg gttttccctc gttaatataa caactaaaaa
    12601 agaggatgtc aaatcaattt tccatgtttt tcatactttt ttaaaataca cgcgttatat
    12661 ttcctgtctc acgcagtttt atttgtctca ttttccacct cccgctataa tagaattata
    12721 gtaggaaacg aggtgctgtt atggaacgaa cacttgttat tatcaagcca gacggcgtaa
    12781 gacgccattt ggtcggaagc atcatccaac gttttgaagc aaagggactg gccattgctg
    12841 aaatgaaatt tgcaacgatg acacctgaac tagcaaaaga acattatcag cacttgactg
    12901 aacgtagttt ttttgatgag cttatcgatt acatgacttc tggtccggtc gtttatcttg
    12961 tgctggtcgg cgaggaagtg atcgatatcg tccgtaagat ggtcggagca acaaaagggg
    13021 cggatgccgt tccaggtacg atccgaggag attatgcact gccggggact gaaaacatca
    13081 tccatgcttc tgattcccgt gatgctgcaa caaaagagat cgcccgcttt ttcccgccat
    13141 tcgatacaaa caaaccgtct gaaaaagcgg cgaagtgata caaatcctaa actgttggtc
    13201 taactgctgg gtttagcaat ggccagcagt tttttctcta aagaattcct ttgattcttc
    13261 cctaatgtat attcgatcac tgcgaaaaac tcttggtgat tttccgattt ttcttcccct
    13321 aaagcagcag aaatcagtcg ttggtaataa tccgccatca atgtttcata aatcactgta
    13381 cttctttctt tatgcgctaa actccgtttc atatcttcat tcacgctacg tgcctttttt
    13441 aagcgaccat tttctatata aaacaaagtc aagttcgtca aaaaagtaaa gagatactgc
    13501 tcgtgattat tcagttgcgc gatccgtcgc attctttttt tgattttcaa ataattattc
    13561 tcaataaaac gatcactgaa tatccaagga aaatctgcta gaatagatac ttcaaattgc
    13621 ccatactctg gtacttgcat caaatattct tcaatgatac gacattcata acgcaaaaat
    13681 tcaaattccg tctcagatac ttctgttttt ttggacatgt gcagattcaa aaccaaatag
    13741 tacagatctt ctgttttctt atagtgcctg cggttattag caatttcttt gtaaggatca
    13801 aagctgtctt cttgaatccg atctagcatt cgtctttttt tctttaattt ttttggctca
    13861 tcaagctgat ttcgtatcca ttcatattcg gcaagtgtca tgttcattcg ctctaaaaat
    13921 gatttcaatg ttccaaacga tacttttttc actgaatttg tctcgatccc gactagcgaa
    13981 gttttagaac agattccttc cgctaattgg gtctgtgtca gctttttgtt gactcgaagc
    14041 ttcttaatca attctccatc atacataata gttctccttt tttaattgaa attaaccctt
    14101 ggcttttcca gaatgcttgc caataagagg aatgattatt tcaatttaaa ttttccgcat
    14161 tcaataaata gatacgttaa aaataaaaaa tacacgaaaa aaataaaaaa atgtttttct
    14221 cataaaaaat accaaccttc aagcatgaga ctgctgtcta atccctgaag gttggtttga
    14281 ttgtttattg atcatttttt tactgctccg cattggttcc tgttactgga tataggatag
    14341 tcgttgtttt attgccgttt tttgaaaaga cactggtcga atgttcacct ttttgctttt
    14401 cgattgcttc attacgttcc gcttcgtttt tatagtcata ttttttcgga tcaatcgtct
    14461 caaatccgtt tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac
    14521 ttaacgcagt cttgactttt tcctgatctt tggagatttc ctcgtcgatt cctgctgctt
    14581 tcgtatcgat gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt
    14641 tgggcgtcac gaagttccca tttcgaaaag caactacttg atcgtgctga gaagataata
    14701 agtctgtacc aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg
    14761 gcaaaacatc gatttctcca ccatattcat gattgactgt ccctttagtg taaccaggca
    14821 tatggatcat caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg
    14881 gatctttacc aagagcactt gctaaatctt tattatctgt attcgagatc ccaaaatgat
    14941 ccccgtaaat cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa
    15001 attgttccaa tgattgatcc agataatggg ccgttcgaac ataattatct actgtgctat
    15061 tgccagtatt caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt
    15121 tggttacgga aagaaatttc gtgtaaaacg gctgttgtag atgttcaaga tattgggcag
    15181 attctctgaa taaatcttta tctaatatcc cataacctaa cgtttcatcg gattcatcaa
    15241 aataagatcg atcaaagaaa ttgtcatacc ctaaattttt atatacatga tctctattcc
    15301 agaaactagc tacattccca tggaaaacag cacttgtata gccttctgtt tgaccaaggg
    15361 tcgctggtgc tgcttgaaag acattatctg acccaagttc tgtgaataat gagccttgag
    15421 gtaaaccaaa tgtgccagtt tccaacatgt tttcagcatc acttgtcttt ccctgtccta
    15481 cttcatgaaa gaaattgtca aaactgatcg ttgcttgatt ctggaaaata ctatttaaaa
    15541 acggcgtgac ttcttgtccg tctaccttca tattgatcaa aaactgttga aaactttcta
    15601 aatgaaggac aatcacattt tttcctttag caatcccaaa tgtttcagga tttggctctg
    15661 cataatgttt ctttgtatag tctaaaactt catcaattcc attactgctt gcatgcgcgc
    15721 gaacactgct agtcattcct gttttgattc cgtcataaat agtgtacgca tcgattccta
    15781 gatatttgac aatataggaa cggtcaaatg tccgctgtag caattgggga cggttggctt
    15841 cgctcaacgc taaatttcct gaaaaaatca atcctgaaag acaggtaaca gcgatagcca
    15901 tcggtttgct gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta
    15961 gcccaatgaa cacagcgata tccaaccaat agatgacatc atggggtttc atcaaagaaa
    16021 aggagcttcc tgacaaccct tgtgacactt tcgaaaagcc taatacagat ttgactgtaa
    16081 tgaaatctgt gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa
    16141 tatcaatgat caaaagaatg gctaacgctg gctttggctt tttaatatac aaagaaaggc
    16201 caaaaaacaa taaggtagtc gcaatcgggt tgatccacaa gataaagtac tggatcgtac
    16261 cttccacacc taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga
    16321 aaacagctgt caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt
    16381 atatcacttc tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct
    16441 aaaaaagttg ttatcttaag atttttttat tctttctaaa gattattcca ttcatttttc
    16501 acagcataaa atcgtttcgg tgtaagcggt taaaatagta aggagataaa aatcttgtgg
    16561 tgaccgattt actctatagt attcaaaagt agtttctcgg tattcgtaca aaaaaaacat
    16621 actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt ctatttttag
    16681 attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga gtccctatca
    16741 aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg ggccatgagc
    16801 ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaca cttaaaaagg
    16861 aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca gctacagtgt
    16921 cctatacaaa gacgacacct gcttatttga agacgagatc gaacacactc aaagaggatt
    16981 tcatgcatta ctcgaagaga tccagcactt gcctgaacgt ccattgattg tttttgaagc
    17041 aacgggtatt tattccagac cagtcgaaaa gttctttcaa gatcatcact tttcttactg
    17101 tttgttaaac ccgttagaag cgaaaaaaca gatggaagaa aacaccttac gcagttggaa
    17161 aacagataaa tcggatgccc atcggctcgc acagactcat cttaaaaatc aacgtcaacc
    17221 aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt tttatcaaga
    17281 aattgagaag aaaatcactc gactgcgcat ggatttacac aactgccttc agttgacttt
    17341 tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga cattgatccg
    17401 tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa aaaataaact
    17461 gatcaacgaa acacgggaaa aaatctccgc gaatcgtgcc gagcaaaagg cagatcaaat
    17521 catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtattc actgtcaaaa
    17581 aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag cactggcgac
    17641 acaaatgatc aaaaaagccg aagcctctcc ttgctttttg ttgtaccaaa cgttccctgg
    17701 gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca cacgatttaa
    17761 aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc attcaggtaa
    17821 gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga aaattatttt
    17881 cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata ttgtcgatta
    17941 ttactacaaa ttaaaaaagc aacctatccc taagaaagat aaggttgcca cagttgcttg
    18001 tatgaacaag cttctgaaat gtatgcacgc tatggttagg gcacatacag agtacgacta
    18061 tgcgtacgcg gtctcagtgg accattaact aaaccactta cagtatacca cttctttgcc
    18121 cagatcgaaa attttttacg aacggggctt tatttagtgt gccttgattt tgtcgctttc
    18181 gtttacgagc tatttttctt gcacctttta cttgactaat cgtaggaaag aggctgtgac
    18241 aaaattcttg tcgcagctcc ctatttcttg atgcaggaca gctttttcac aacctctcca
    18301 ctaaacggtg ttcaatcagc tgaccttcgg tattagctca aaaaacggca aaatatgagg
    18361 agctattttc ccgttttttt ctctaatact caggtcaaac cgctgatttc acaacctctg
    18421 attcacggtg ttccagaagt agcttcttcg gaaataagcc gaaattttac aaaaacttga
    18481 gaagcacttt tcgtaaattt cttcttattt cttgaagctg accacttctg tcacaacttc
    18541 tgtttttcat tgtccatagg tttgatattg tccttctgat gactcattcg taatgattga
    18601 ggcttgattt tcttcatttc ccgttatcgt ttgtccactg ccagaatctg aagggcttag
    18661 ttctgtcgac tcgttttttt gagaagaagc agttgttttc ttgacttgtt cgttggtctt
    18721 tttccattcc gctagtcgat cggccaccac ttttttgtcc actttttcag aataagctgt
    18781 aaccactttt ccatttttca aacgtgctac cttgtcgcca tcatgagtca aaagcaaaac
    18841 gaccaaatgt ttgtctttga attgatattg tctgagagaa atcgcactaa gtacgtcatc
    18901 gatattgata aattgatttt cttcttttcc ttttggcaaa tagatgtcca attcaccttt
    18961 ttcgtaattg gcagttaatt tatattcctc ccaccaataa gttgagaatt cttttttgaa
    19021 attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag caagatctgc
    19081 tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac cgaataacca
    19141 aaagagaaga aacggtcttt ttttcctcat gccttcctcc actcctattt aattttgact
    19201 attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag atcgtaaata
    19261 attgaacttc tttttcctga agcggtaaga acgggaattt tcatacattc tctcttggct
    19321 attctttaag cttcaaatcc atttctgtct gatacatctt tgaaccaatg acccgatttc
    19381 ttgatcgttc tttttccatc ttgtgctaaa tctactgaaa taaaaccgta acgattctta
    19441 taggcattgg tccacgacca gttatccatg catgtccaca tatgataacc taaacaattg
    19501 gcaccttctt gaatggcttg atggacatat ttcagatgag attgaacaaa ttcgatccga
    19561 taatcatcgt ggatcatacc atcttccttg ataaatcgtg cttcattttc tacacccatg
    19621 ccgttttcag aaataaaaca gtcgatattc ccataatgat ctcgtacatt gatcaaaata
    19681 tcatagattc ctttttcata gatctcccat ccgcgataag gattcatttt acgaccaggc
    19741 atttcataat tgtcgaagta atcctcaggc ataggaccgt ttttagtatc gattggtgtt
    19801 tcttttgctt ttattcggcg tggttggtaa taattaattc ccaaaagatc tactgtgttt
    19861 tctttaatga tcgtcaaatc atctggatga atctccggca ttagatcgag ttcttttatt
    19921 ctctcaatca attcactggg aaactcgcct tttacagctg gatcaaggaa tgaacgattg
    19981 aagaacgcat ctgctattcg tgctgcttta acatcttcgg gatttgattc gtctcttgga
    20041 taactcggtg tcaaatttaa gatgattccg atcacaccgc tttgatccat ttcatggtaa
    20101 gctttgatgg ctaatgctga agccaacgct tcatgataac caacttggat tgcctttttc
    20161 atatctgatt cttccggata atgccatcga tacaaatagc ctgcctcgac tggaacgatt
    20221 ggttcattat gggtaaacca tttttttact ttcgagccaa acaattgaaa acaacttttt
    20281 gcgtattttt cataggcttc aaccgtttct cggttcaacc atccgccttt ttcttgcaga
    20341 tacatcggca tatcgaaatg atacagatta acaaagggtt cgataccgtt ttcaatcaac
    20401 tcatcaaaat agtgatgata gaaacgaacg gcttcttcgt tgattgttcc agattcatca
    20461 ggtatcaaac ggctccattg gatagaggtt cgaaatgaat tatgcccagt ctgtttcatc
    20521 aactgtacat cctcttgata cttcgtataa acttgcgagg ttttttcagg tcctacttga
    20581 tcaaagaact tttgcggttc gtttttgtac caataatcaa aaatattctc tccttttcca
    20641 tcgcctgaaa ttcttccttc tgtttgtgga ccacttgctg cagagcccca ccaaaaattt
    20701 tttggaaatt gatatctttt catcaaagca cctctttttc ttttttagtt gattccatgt
    20761 atgatatttt ccatcacaat cactttttca ttttcttcct cttctttttt gattcgtttc
    20821 aacagctgtt ctgctgctcg ttccccgatt ttatctgttg gtggcgtgat cgtgattttt
    20881 ggaaggatac tcagatcgtt tttatctaaa taatcatagt gactgaacgc aatatccttc
    20941 ccgattctga caccttcttg attgaaacat tcgatggcac caatcgacat gttgtaattg
    21001 ctgacaaaaa tagcagttgc ccctttttct aataatgctt ttgccccgta atagccgctt
    21061 tttctagaat agtctcctgt aaagatctct aaatggatag acggatcctt ttgtaccgct
    21121 tctttggctc cagctaaacg ttctcttgct acgtaatctg tttgagcggc agcaataaat
    21181 cctattgttt tatgtccttg tccgccatga tgagcaatca cttttgctac actttctcga
    21241 tcattcacta ctattgtatc aatacctgct ggcccacccg gacaattcaa tgcgacaact
    21301 gggatactca tttttgtcag catttcaaat ccagaccact cttcttctga taagaagacg
    21361 atcaatccat cgatcgcatg cttcatcagg tattcgattt tttgttcgat tttttctggc
    21421 tgatcggcaa acccgcttaa taataagcta tacccatttt cttcagctac tctttcgatc
    21481 cctgaaacaa cctccgaacc aaatcggtta gatatcccgt ttatcaacag tccaatagag
    21541 aaacttcgat tattcttcaa tcctttagca atgatgtttt ctttatagtc caacgctgaa
    21601 atcgcttctg atattttttc catattcttt tccttcagct gctgaccgtt taaatagcgg
    21661 gaaactgttc ccactgaaac accagccatc ttagccacgt cacggatcga tgccatacat
    21721 tttcactcct gccagttaca tttgatttct ttttcttgtt gttcgaccat gatgaccgct
    21781 ttctttgcat aatgccgctt gtcatcaata tccatataac taaagtcttc agaagtgagt
    21841 tcaaaaacaa gcagtctttc ctcttgagac cgcaattcat ctttacgaaa agctttcaat
    21901 aatttcgttc tttggatgac aacgccgtca attaatcgga catagacaag agtacttgtt
    21961 ttacctgaaa cacttcctgt atttttaact ttaattgata ttattacttt gtcaatatcc
    22021 gttttgactt ccatctgact gtattcaaat gttgtatagc ttagaccatg tccaaaagga
    22081 taaaatggac ttccgcttaa atcataataa ttctcttgtt tagaagcagc acgctgatag
    22141 taataaacag gtagttgccg actatttcta ggataagaca aactcaatcg tccgcttggg
    22201 ttatttcttc cagaaatcat ctgcgcaatg gcttttccac cctcctgtcc aggaaaccat
    22261 gctgttgcaa tggcattcgt aaatgactcg attttcatca aatcgtaggg tcttccttgc
    22321 acaagtatcg tgatgattgg cttgcctagt tgatacaatt gttcaatcag ctgttcttgg
    22381 tatccaccta aagaaagaga agcaacatct acattttctc cagaatccat gttgatccct
    22441 ttcgatgaaa ctgccccgtt acgaagaaat tccatatcaa agttccgcgc acttgatcca
    22501 cctagaactg caatgatcac atctgagttt tgagcaaggg tcaccgcttc taataatttt
    22561 tcttcctgat cctcctgatt tcttacttca catccttgtg cataagaaac ctttgattgg
    22621 ggaaactctg tttttatctc ttgtactatc ttttttttca aacggtcttc tgtttgtggt
    22681 gcagagtagt ctcccaacaa gtggtaaaca tcgtgagcat ttggacctat aacagcaatg
    22741 ttttttactt gttgatccaa cggcaatata ccgtcatttt ttagtaaagt gatactttct
    22801 tttgctgtct ctaaatttaa gtgctctgat tcctccatga gatgatccca atactccttt
    22861 ggatcgtttg caaatggctg ttcaaataaa cctaataaaa atttgacaga taaaactcgg
    22921 cgaactgctt gatctagtag cttttcatct acgacttgat tatcaatggc ttctgcaatc
    22981 ttggtatagg tctcatccca caaactgaga tcgattcctg ccgctaatgc atatgctgca
    23041 gcagtctttt tatctgtaaa aacatcggat aaacgatcca aagccacacc gtctgccata
    23101 acgattcctt gataaccgat ctcttttctt aatgtttgct caaaaagcgc tcgattgata
    23161 tgacaaggta tcccgtcgat gtcattataa gcagccatga cacctactgc atttcttgta
    23221 ctcttcaata atggtccgta aatatcggag aactctctgc gtccaatcgt tactgtgcct
    23281 gaattatgac cgcctaaagc ttctccttgt gcgatacagt gctttaacac aacaccgatc
    23341 tgatccttct tcttattgat ttcctctact gtctgatcaa gaaaagaaac tttttctgaa
    23401 ataatctctc cttggaaccc tcgaacgacc gcttcagaaa aacgagctga gagaatcgga
    23461 tcttccccaa agcactcttc tgtccttccc caacgaggat cttttgccag atccaaagtc
    23521 gacaccagcg ctaaatgaac acctttagca gccagttctt ttgccatcaa tcgactcgat
    23581 tgttctaata atgctacatt gaaagtattt ccacggccga tattcgttgg ataagaaatc
    23641 ccatccaagc cttgatgacc atgtggacat tcttctacaa gcaaaactgg aattttccat
    23701 cgcgaatgct gtagtacata ttcttggact aggttagcca ccttccagct ttcttttgca
    23761 ggaatcccat tcttataatc tgttttagac cacggatcag agcgaaacag accgtataag
    23821 gcacccagac cgcctcccca tttaacatgt tcttttaact tttctgttaa ttcgatcttg
    23881 tctccgtttt tttcataaca ttcccaacca tacaaatgct ggtttacctg tcctactttt
    23941 tcagcaatat tcatcctcga aagtaaatct tctactcttt ggcggatggt taactttggg
    24001 ttttgatacg ggtaatcgta caccttaata tcctccttct gccaattttg ccttattagc
    24061 ggctaataca aatggcagga ataaaaatac atctagaatg ataagtatta ctgttaagac
    24121 tgctgctcgt atatcgcctc ctgtagcaat aaaagattgt ataaccggtg gagtgatcca
    24181 aggtgcagca gcaactgttt tactgatgat tccaagactt gtggctgcgt aggcaataac
    24241 caagttaata gatggtataa tagcgcatgg aatcatatat attggattca tgactactgg
    24301 taatccaaac ataattggtt ccgtaatatt aaataaacaa gtagtcagac caaatttagc
    24361 gattgatcgc tgttcttgac gtttagaagc ccacaagata gcaataacta acggtaaaat
    24421 acacccgcca cctccgatca atccaaatgc attggtaaag ggctccgtaa taatattagg
    24481 aatttctttt cctactgaaa aagcatccat gttttcctga atagactgta acaaaggtgc
    24541 ttgccgtata ggagccaaaa tactcgaacc atgcatacca aacacccata agaaatcaga
    24601 gaaaaactgt aaaaataaga tgcctgggac ggataaaaca aaagctttca atggtgtctg
    24661 aatcgctaat tcaattaatt gcggaacatt caaaccagtc gtatggttta aagtaaaaac
    24721 gacaactgaa aaaaataaaa tgacaactat ttctggtact aaagaattaa atgacgcagc
    24781 tactgccggt ggaactcctt ctggcatcct gattttaaaa ctcttcattt tcgctaattt
    24841 gataaataat tctgtactga caagggcagc aatcatcgcc gttgctaagc cagacgaaga
    24901 agtcagactt tgtaagaaaa cccctgtagc ttctgctgta tttccgtcca tcaaagtgac
    24961 tgtacttgat aaagacatca aaataaacat tacggctgta cttagaattc ctgcatgaac
    25021 aggactaaat gatggatcat caatcacgcc tgtttggata tagtgggttg ccaaattcca
    25081 gccgatattg taacacacga taatagctaa aatactcatc gtaccattat tcacgattga
    25141 agcaagttca gccaatggac ttagatcaag tattctttgg actgttgcat ttgaaaaaat
    25201 cagagcattc agcaaaacca tgacagaagc agccattact aacggcatag taacgataaa
    25261 actgtctcga atggccagca agtgtcgctg gctactcatc ttagatacga ttggtagcat
    25321 tttttcagat agtctgtcta cgactttcat tatttcatct cctagattct aaaattgaac
    25381 cggttcattt ttaatatagc acttttttta aagattgcaa cacatttttt taaaacgctt
    25441 tctaaaggtg gtggattttt tatgactttt gaaaaattat tctatttaat ggtattttgt
    25501 gaattttttc tcttccatcc gtttgaagtt ttactttcca atggtataat ttctttatcc
    25561 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
    25621 ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa
    25681 ccgattcttc tgtcttaaaa gcggaaaaag cagatatcac caacagtgta tatacgctaa
    25741 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
    25801 aatggacacg cacgtatcag acacaaggag ctgattcttc tgaatcatct gaagaaaaaa
    25861 cagtgctgac agatatcaag attactacca aagatgacga atatattatt tcaggaaaag
    25921 aaaataaaaa agaagcaaca gtcacattta ccaaaatcgg taattaccgt ttgaaggata
    25981 aggatggcaa catctacagt ctatagtgcc atctgttgga tcttcttttg tttctcccct
    26041 tcttttctgc tatagtaaag aagcaagatg actgtaaagg agacgaaatg gatgatcgat
    26101 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgatcaa
    26161 ggaagtttga gtatatgcga caattgccac aaactagtca aagattcttt taaatcgttt
    26221 gaatcttatc ctgtcaaaaa ttatgtaaaa agcaaaagaa gataagcaaa atcaagcaaa
    26281 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
    26341 aaaactggaa tcagtaaaac aaacagtgga tcatttcagt tcacaaatca aagagtaacg
    26401 ttttatttat ttcacatctt tgaatataaa ataaccaaac gatagaaaga cttattctac
    26461 atcacgtagt aatttttctt tcccgtcgtt tggtttttta cttttagcct aattcttgac
    26521 ctttcgcatg ctgattatct gtaaaatatt ttccagcagg ctttccttcc aattcagcta
    26581 atttttcttg gatctgacga atcacctttt ttgcatcttc tggattttct acaaaatcga
    26641 atgtatcccc atccacttgt attttaggac atacatcaaa atcctcatac cattgattat
    26701 agcgggtgtt cagtgtttcg taataggtgt ataacgtttc atcgctttct agctgttcat
    26761 agtcacgccc gcgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga
    26821 ccatcaaatc aggacgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta
    26881 ataagctatc gtactgctgt acttcgatat ccgttacccg acctaaatca gcatttagat
    26941 ggaacaacag actatcttcg tagatggagc ggtctagcac attgtcatcg ttcactaaag
    27001 catttttcat tgctaaaaat cgcttattca aaaagaagat ttgcaataaa aatgcgtact
    27061 gctcaggatt tgaataaaaa agcggcaata cttcgttatc ttcgacattt tcataaaatg
    27121 gtttagaatc aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg
    27181 ttcctgaaat caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt
    27241 aagtttcaac aggcaatttt tcaaaatcat attcacgtac agcttggtct gattttaaat
    27301 acgtgtgaag actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacgatga
    27361 tcatcggttt tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg
    27421 tttgatgctc aaagtcaagg atagccaaaa caaattctgc atttgtcaac tcttccatat
    27481 ctttttcgta tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt
    27541 gatgacgtgg agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac
    27601 gacttactcg atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca
    27661 ttattttgcc tccttcaata ataactgatt tttaatagat actgctcgta attcattgac
    27721 cagttccaat ggattgttgg aaaagatcac ttgatccaac cattttttat ccataaaatc
    27781 ctcttttgca aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa
    27841 tattccgatt ggcttatcag gtgtcaggcc gatcgctatc caagaaagaa gttgcgaaaa
    27901 ttcctccaat gtacctagac ctccagggaa aacgaggaca gcatctcctt tttcaaataa
    27961 taataccttt ctttcatcaa ttgtctctgt aggaataaat gtagtaacat ctttcccagg
    28021 cagctcagtt tcaaaaaggc catttggata gatacctgtg acttttccgt tattttccag
    28081 tactgtttta ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat
    28141 ttcttcttgt gccatatatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt
    28201 gccaaaacgg gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa
    28261 gtctatcttc attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta
    28321 gtggtatata gatcaaacac cactatatat agtatttgca aaaaaataat ttttcagcta
    28381 ttttacagta accaaaaaaa taaataacta gtaaatataa aattgaaacg acttacttat
    28441 agaagaaatt gggatttcgt cctgcatgtc aataacacgc attatttaaa tagtcacaca
    28501 cttgtttctc ctattaatga aaaatccatt ttattctcgt gttatgtttc accaacgcct
    28561 gatgtttctc taaataaaaa agccgacgaa ccaaataatc gaaatttgat cattttggtt
    28621 catcagcttg ttcccaaacc tttgtattaa cgataaatag ctggattcgt aaaactatca
    28681 cttggatcat cactgctgga agaaaattcg gtgataaccg cgcctgttgc acctgcctgg
    28741 aaccagtgtt taatattttg ttcgatcgtg tactgatctc cagcgtgaag tatgatttct
    28801 ttttttgccg tatagtaacg ttcttgcccc tttggaataa tacaggaaag tttttttgtt
    28861 tcttttcctt cgacataaag atatactaag ccttttcgac aacgaaaggt ttcctttttt
    28921 ccttcattct catctcgagg tgggtgccga tgctctggac aagtttgata aggcaataat
    28981 accagttctt tcccacagta tcgctcatta ttttcataaa caaataaatt caatccttca
    29041 gtctcgattg ctcctaatcc aaaatctgta tattcgattc tttctaattc tgtttctttc
    29101 aatactactc ctgaatctag gaacatttgt tgtactcttt tcttgtaatc cattctgttc
    29161 ctcctaactg attgtcaagt catcccaaga tatctcttct tcctcttttt cttcttggat
    29221 gacttctgca tttgggtcaa ctggtttttt caataataaa atagttattc ccgtagcaaa
    29281 agaaccagct aacaatgcac caatgaatgc ccatggtctg gtcatcgtag cgactgcaaa
    29341 cattcccgat gccggaatac ggctatctgc gccccaaaac atgctcaatc caccgccgac
    29401 tgccccaccg actcccgttg ctatgacatt gcgtgccaag tcgttcaata cgatagggaa
    29461 acatccctct gtgatctggc agatccccat aggaaaagcg gtcttcaatg tatcgatttc
    29521 ttgacgattg aatatatttt tacgaatgat tttccctaag aaataagcga tcgtcaagcc
    29581 gaatggggtt accatcgaag ctaaaatcaa tacagtgatt ggttcataga ttccttctga
    29641 aaatcgtgcc acaccccctt atccagaata aatggtaatc cagaaacagc cgggtatctg
    29701 cggtaataag cctaaccaaa cgaaaaatat gagaagctaa tatgagaagc tatttttgtt
    29761 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttttt ttatttatgt
    29821 ttcataatca atatggtaag gatgcagctc cgtatggtag accgcctccg aatattcaat
    29881 catttcccca atttgcgtag acgatttccg tatccgtttg atgccgatct gtgcgtctgt
    29941 tttcagaatt tcttgttctt cttctgttaa aaatactgcc gagaaatcat cagaaaatga
    30001 gtcaatagta tatccttttt tcgtcagcaa acgatacaaa gattctttgc caaattcttc
    30061 tctgctgaat tgcatcattt cttgtggaag ataatgaaca aaataaatat aaggctgatt
    30121 gttcaaataa tataagcgct taaaacaaac agcttgctca ccgaaaaagg aaaacaaagg
    30181 atcttccttc tttatatgga ccaattgcaa atccagcgtt tctttccgga cttcatatcg
    30241 ggagttctcc aagatttgcg taaagcttgc cgctttggat aatcgattgt aaggacgatt
    30301 gcttagaacc gtcgtcccac gaccactttt ctttgcgaca tattcatctg atgccagcag
    30361 ttcgattgcc tttcggacag tgattttgct gacttgaaac agttcttcta attcactttc
    30421 tgtcggtaac atcgaaccga ctggatattt tcctgagaaa atatcttctt taattttatc
    30481 tgcgacttcc aagtatagta cacgtgtcct tttcataacg tcaccctttg ctttactatt
    30541 actaaaagaa tagcacataa aaaataaaaa aagaacacaa atcacatgtg agaagtgtgt
    30601 tctttttcat tcagctgctt taattacaag tttacgccat ttgaggcaat tacttcttta
    30661 taccaatcaa aagatttttt ctttgatcga ttcagtgtcc catttccttc atcgtctaga
    30721 tccacataga taaagccgta acgtttactc atttctcctg ttgatgcact gacaagatcc
    30781 atacatcccc aaggcgtata gcccatcaaa tctacaccgt ctgctactgc attagccatc
    30841 gcttcgatat gacgacgtaa ataatcgatc cgataatcat caataatcgt gccatcctct
    30901 tccatatgat caatggctcc aagaccgttt tcaacaatga ataatggttt ctggtaacga
    30961 ttgtataatt cattcaatgc gattctcagc ccttctggat cgatctgcca gccccattca
    31021 cttgcctcaa gaaacggatt ttttactcct cccagcaagt tcccttctga agttgctgct
    31081 tgtggatcag tctcattgat tgccgtagac atataataac taaatccgat gtagtccact
    31141 ggatattctt ttagcaactg gaagtctttt tccgtttgct ctaatttttc cggatcgact
    31201 ccatattttt tatgggtacg cgctgtgtat gcagggtatt ctccacgtac ttggacatcc
    31261 gtgcagaaaa aattgaattc ttggttttgg atcattgttg ctgcctgatt gactggattg
    31321 gcatcgatgc tgtaagttgt tgcatagata atcatgcagc cgatttggat atccgaacgc
    31381 aattcgtggc cgatttttac ggctttgcta cttgcaacga attgattatg ccatgcttgg
    31441 aaaatatttt ggtactctcc tgctccattg ctttttacta atccttgact taaagctgga
    31501 aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat atttgacttt actgctgtat
    31561 ctctctaata ctgtctgagc aaatcgttca tagaagtcga tcagttttcg atttttccag
    31621 ccaccgtatt cttttgccaa atgaagaggc atttcgtaat gagaaatcgt gatcacaggt
    31681 tcaatatcat attttaagca ctcatcaatc agctgatcat aaaattctaa tcccgcttcg
    31741 ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga aaaacgataa
    31801 catttgaatc ccatttccgc aaacaaggca agatcttctt tgaaacgatc gtaatgatca
    31861 attcctcgat gattcgggta aatatatttt tcttgatcaa tcgtccagtc aaattcttca
    31921 ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag catctgcaac ggatagccct
    31981 tttccgtctc gatcataagc cccttctaat tgattggcag cagttgctcc accccataaa
    32041 aatttttctg aaaaacgtga ttgcatcctg atttcctcct tattttacaa ttgccagaac
    32101 atcttcgttt gcaataatct ctttttggtc taattcaagt atatcggcaa atgcatcagt
    32161 attcgtaatg acaactggag tgattacgct atagccagct gctctaattg tttcgatgtc
    32221 aaaacgaatc aataaatctc ctgctttgac gagatcccct tgtttgacta agatctcaaa
    32281 gccattccca tttaattcta ctgtatccat cccgatatga atcaaaatct ccgctccgtc
    32341 agtcgttgtg atccctacag catgtccagt tggaaaaata gtcgtgatct ctccgtctgc
    32401 aggtgcaact agttctccta cttctggatc aatagcgata ccttttccca tcacgccaga
    32461 agaaaaaact tggtctggta cttcactcaa tggcagtact ttacccgtta aaggactttt
    32521 taaggtatgc cgtgctgaag taatcggttc tcctgcattc gcatgtttgt tttctaattg
    32581 attgtcttgt gtttgttcgt caaatcctaa aatcaatgta cctacgaatg caagaacaaa
    32641 agcaattcct gtagctatta ctgctactgt cacatttgac tctacaccat ccaccgtatt
    32701 aatgaaagtc ggaatcgtca ataagctgac caaactgctg gagaaagctt ttactccgga
    32761 aaatccgata atagctccac caatacctcc agaaatacaa gcggcaatga atggtttctt
    32821 caaaggtaat gtcacaccat acacagttgg ttcagtgatt ccaaataacg aagtcactgt
    32881 agaagaaatc cctaatgctc gaagtttcgt atcttttgtt cgcaatgcta cagctaaagc
    32941 agccccacct tgtgccaaga tggctggaag aagcattggc atcaatacat caaacccata
    33001 ttgttcgata ttcaaaaaca tgatcggtac gaatccccaa tgcatgccga acatcacgaa
    33061 tacttgccat aaacttccca tgattagacc tgctacgatt ggactaaatc cataaatcga
    33121 gttgaagaga ccgcctaaag cattaccggc aaccgtcccg attggtccta aagcaagaaa
    33181 tgttaatgga accatcacta ctaaaacgac aagcggaact aaaatcattt gcagaaattg
    33241 cggaatcact ttttttacga atggctctaa tttactttga acccatacgg ctagaatgat
    33301 tggtatgaca gaagaagtat aaccagatgg tgaaagaatc actgggatcc caaagaaact
    33361 gatagcttct cccgcaccag caactgctgt gatagctgga tacacaagtg ccatagcaat
    33421 tgctactgct aaaaactggt tggctttgaa ctttttagct gctgtaaaag caagcatgac
    33481 aggtaagaag gtaaagacac catctgctgc agcaaacaat atttgataag taccgctatc
    33541 agctgtcagc cagcccatca ccgttgccaa tgataagaat cctttcaata ccccggcggc
    33601 tgccatcgcc cctagaaaag gtgtaaatat actagagata atatcgatca gacgattcaa
    33661 aatatttttg gactcttctt ttccatcctc cgaaactgca agaccttcct catccacgat
    33721 cgattgataa acatctgcta cattactgcc aatcactact tgatactgac cgccactttg
    33781 tacgacttgg atcacgtccg ggtcttcttt taacttttgc gtatctgcta aagattcatc
    33841 ttttaatctg aatcgcaacc gcgtagcaca atgtactaaa cttttgacat tcttttgtcc
    33901 tcctaccgct tcccatacac gacgaccaac tgcttgatta tccattgttt tttccttctt
    33961 tcttttttat ttaaaaaata aaaacccaga ttgaaaaaaa cagtcacaaa cattcgtgaa
    34021 cctatttttt caatccaggt tttgcctgcc atacagtaac aatccttatg attggatgct
    34081 ttttttctca cttactactc tttgaatatg aatcgttaaa taaatctgct ctgcgatccc
    34141 catttcctgt ttttgcgttt gtcgaagata ctcattgatc cgttcgacac aatgaaacgc
    34201 ttttggatac tttccttgaa ctaatgcgta gagaaattca tcttgctcgt cagatccttc
    34261 ccctttgaga taacgctggg caaaaaactg cagatgagta atgatacgct gataattcaa
    34321 agattcttca tcgaacacga tgccgaagaa tttactgatg atactcagta tatctcttac
    34381 cattgtggta gcggtcatcg tcacttgcat gtcttcgttt ccttgttggc tattcacaaa
    34441 atgaaaggca ataaaaccgg cttcatcctc agaaagttcc acacccgtct tttgtttcac
    34501 gaacaaaaga gcctctttcg ctgcttgata ttctttcgga taaaattttt ttgtttcaaa
    34561 taataatgga ttaggcagtt ggatattttc ttttgctcgt gtgatcgcat aatgaagatg
    34621 atcggtcaat gtcagataaa tattaggcga aaattcaact tttagtttat cttcagccag
    34681 ttcgactact tgtttgaccc attcgatctc ctctgccgga atatcagcca tcagctgctg
    34741 gaattggttt ttagcaaaag aatctcttaa aacaaattct ttttggaccg cttctggcga
    34801 aatctcgtcc ccaatctttt tttgaaaagc tagtcctttt cccatacaaa cgacttcttc
    34861 cccgaatcca tttttcgaaa tgacgacatt gttattcaa
//
LOCUS       DACDCJ010000022        34662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDCJ010000022 DACDCJ010000000
VERSION     DACDCJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.022"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IV038_001062"
     CDS             591..1280
                     /locus_tag="IV038_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8341297.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IV038_001063"
     CDS             1267..2475
                     /locus_tag="IV038_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8341298.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IV038_001064"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IV038_001064"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP8341299.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV038_001065"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IV038_001065"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP8341300.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IV038_001066"
     CDS             complement(6578..7273)
                     /locus_tag="IV038_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP8341301.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV038_001067"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IV038_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP8341302.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IV038_001068"
     CDS             complement(8472..8663)
                     /locus_tag="IV038_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP8341303.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IV038_001069"
     CDS             complement(8684..9574)
                     /locus_tag="IV038_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP8341304.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IV038_001070"
     CDS             complement(9561..10328)
                     /locus_tag="IV038_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8341305.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV038_001071"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IV038_001071"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP8341306.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV038_001072"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IV038_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP8341307.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV038_001073"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IV038_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP8341308.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IV038_001074"
     CDS             14944..15282
                     /locus_tag="IV038_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341309.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IV038_001075"
     CDS             complement(15450..16208)
                     /locus_tag="IV038_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8341310.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IV038_001076"
     CDS             complement(16226..17044)
                     /locus_tag="IV038_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP8341311.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV038_001077"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IV038_001077"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP8341312.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV038_001078"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IV038_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP8341313.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IV038_001079"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IV038_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP8341314.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IV038_001080"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IV038_001080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP8341315.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IV038_001081"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IV038_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP8341316.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IV038_001082"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IV038_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP8341317.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IV038_001083"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IV038_001083"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP8341318.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IV038_001084"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IV038_001084"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP8341319.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IV038_001085"
     CDS             27086..27385
                     /locus_tag="IV038_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP8341320.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IV038_001086"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IV038_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8341321.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IV038_001087"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IV038_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP8341322.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IV038_001088"
     CDS             28352..30325
                     /locus_tag="IV038_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP8341323.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGESAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IV038_001089"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IV038_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP8341324.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IV038_001090"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IV038_001090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP8341325.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IV038_001091"
     CDS             33204..34496
                     /locus_tag="IV038_001091"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP8341326.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10562 a         6731 c         6856 g        10513 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gatgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg ggggggaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aa
//
LOCUS       DACDCJ010000023        34288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDCJ010000023 DACDCJ010000000
VERSION     DACDCJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.023"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..312)
                     /locus_tag="IV038_001092"
     CDS             complement(<1..312)
                     /locus_tag="IV038_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341327.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            434..1018
                     /locus_tag="IV038_001093"
     CDS             434..1018
                     /locus_tag="IV038_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341328.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1029..1274
                     /locus_tag="IV038_001094"
     CDS             1029..1274
                     /locus_tag="IV038_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341329.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1280..2002
                     /locus_tag="IV038_001095"
     CDS             1280..2002
                     /locus_tag="IV038_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341330.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2133..2876
                     /locus_tag="IV038_001096"
     CDS             2133..2876
                     /locus_tag="IV038_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341331.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2882..3247
                     /locus_tag="IV038_001097"
     CDS             2882..3247
                     /locus_tag="IV038_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341332.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3263..3793
                     /locus_tag="IV038_001098"
     CDS             3263..3793
                     /locus_tag="IV038_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341333.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3955..4263
                     /locus_tag="IV038_001099"
     CDS             3955..4263
                     /locus_tag="IV038_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341334.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            4259..4678
                     /locus_tag="IV038_001100"
                     /pseudo
     CDS             4259..4678
                     /locus_tag="IV038_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4815..5615
                     /locus_tag="IV038_001101"
     CDS             4815..5615
                     /locus_tag="IV038_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341335.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            5652..5834
                     /locus_tag="IV038_001102"
     CDS             5652..5834
                     /locus_tag="IV038_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341336.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            5945..6262
                     /locus_tag="IV038_001103"
     CDS             5945..6262
                     /locus_tag="IV038_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341337.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            6265..6497
                     /locus_tag="IV038_001104"
                     /pseudo
     CDS             6265..6497
                     /locus_tag="IV038_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6612..7091)
                     /locus_tag="IV038_001105"
     CDS             complement(6612..7091)
                     /locus_tag="IV038_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341338.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7211..8548
                     /locus_tag="IV038_001106"
     CDS             7211..8548
                     /locus_tag="IV038_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8341339.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8571..9098
                     /locus_tag="IV038_001107"
     CDS             8571..9098
                     /locus_tag="IV038_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341340.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            9085..9648
                     /locus_tag="IV038_001108"
     CDS             9085..9648
                     /locus_tag="IV038_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341341.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            10070..10807
                     /locus_tag="IV038_001109"
     CDS             10070..10807
                     /locus_tag="IV038_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341342.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(10824..11240)
                     /locus_tag="IV038_001110"
     CDS             complement(10824..11240)
                     /locus_tag="IV038_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341343.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11632..12879
                     /locus_tag="IV038_001111"
     CDS             11632..12879
                     /locus_tag="IV038_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341344.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            13147..15537
                     /locus_tag="IV038_001112"
     CDS             13147..15537
                     /locus_tag="IV038_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP8341345.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15586..17421
                     /locus_tag="IV038_001113"
     CDS             15586..17421
                     /locus_tag="IV038_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8341346.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17496..18353
                     /locus_tag="IV038_001114"
     CDS             17496..18353
                     /locus_tag="IV038_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8341347.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            18343..19452
                     /locus_tag="IV038_001115"
     CDS             18343..19452
                     /locus_tag="IV038_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8341348.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            19791..20591
                     /locus_tag="IV038_001116"
     CDS             19791..20591
                     /locus_tag="IV038_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341349.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            20679..21485
                     /locus_tag="IV038_001117"
     CDS             20679..21485
                     /locus_tag="IV038_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341350.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(21507..22934)
                     /locus_tag="IV038_001118"
     CDS             complement(21507..22934)
                     /locus_tag="IV038_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP8341351.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            23255..24124
                     /locus_tag="IV038_001119"
     CDS             23255..24124
                     /locus_tag="IV038_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP8341352.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            24144..24491
                     /locus_tag="IV038_001120"
     CDS             24144..24491
                     /locus_tag="IV038_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341353.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            24504..25766
                     /locus_tag="IV038_001121"
     CDS             24504..25766
                     /locus_tag="IV038_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341354.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            25846..27222
                     /locus_tag="IV038_001122"
     CDS             25846..27222
                     /locus_tag="IV038_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8341355.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            27219..27848
                     /locus_tag="IV038_001123"
     CDS             27219..27848
                     /locus_tag="IV038_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341356.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            27867..28346
                     /locus_tag="IV038_001124"
     CDS             27867..28346
                     /locus_tag="IV038_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341357.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            28848..28901
                     /locus_tag="IV038_001125"
                     /pseudo
     CDS             28848..28901
                     /locus_tag="IV038_001125"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28905..29504
                     /locus_tag="IV038_001126"
     CDS             28905..29504
                     /locus_tag="IV038_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8341358.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            29520..29996
                     /locus_tag="IV038_001127"
     CDS             29520..29996
                     /locus_tag="IV038_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341359.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            30013..30915
                     /locus_tag="IV038_001128"
     CDS             30013..30915
                     /locus_tag="IV038_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341360.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            30988..31179
                     /locus_tag="IV038_001129"
     CDS             30988..31179
                     /locus_tag="IV038_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341361.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            31343..31930
                     /locus_tag="IV038_001130"
     CDS             31343..31930
                     /locus_tag="IV038_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341362.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            31930..32829
                     /locus_tag="IV038_001131"
     CDS             31930..32829
                     /locus_tag="IV038_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP8341363.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            32829..33743
                     /locus_tag="IV038_001132"
     CDS             32829..33743
                     /locus_tag="IV038_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341364.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            33795..34010
                     /locus_tag="IV038_001133"
     CDS             33795..34010
                     /locus_tag="IV038_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341365.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        12863 a         4751 c         6794 g         9880 t
ORIGIN      
        1 ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta gtagttccat
       61 ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt ctaaaatttt
      121 cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa ttaaatctaa
      181 aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat aaaaactact
      241 tctaacttct tctaataatg gaataattct aggattttta acttttttta tttcatcatc
      301 taatataaac atataaatct ccttttttta tttgtatgac aagtttacca taaaaaaaaa
      361 ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat atgtagagtg
      421 agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc ctgataatcc
      481 tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat tttatctaat
      541 tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt tcgatataga
      601 aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag attgggaaga
      661 aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac aagatgctga
      721 tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat tttatcttat
      781 gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa aaacaatcaa
      841 ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat ataaagatgg
      901 gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg aacttaggga
      961 atatgtgcag aacccatata attttcaata tttagcagga agaataaatt tagtttagga
     1021 gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt gaaggtgtta
     1081 ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc attcaaaaaa
     1141 atccacaata cctattattt agtctttatg cttggattgt acaaatggtt tgggtttcca
     1201 tattgatgct gttgttttat tggatagtac gggttattct tttattgtta aacaaaagaa
     1261 aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt tatcgacagc
     1321 agcattaata acccttacag catgtggtac aactaatagt gctaataata acaaagacac
     1381 aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa caacaatgtc
     1441 ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta ataaaggtca
     1501 aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg aaaattatgg
     1561 gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg attgtccaga
     1621 ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta gttatgctcc
     1681 aacattaatg catgatatta ccataaataa accagcttac gagtattacg cagttgctgt
     1741 agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg ctgatgagaa
     1801 caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta acggcacact
     1861 atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg aaagtacaac
     1921 taaatattat atggatttgg gagcatataa attatatcaa caatcagagc ctgttatgga
     1981 cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt aaaattaagt
     2041 gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga aacacacaag
     2101 ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca aatgaagctc
     2161 atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa ggaatttata
     2221 aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta aaggcaacaa
     2281 gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca agtgaagaaa
     2341 tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa aaacatctac
     2401 tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa aaattaagaa
     2461 ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc ttaaatgact
     2521 acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac acacaataca
     2581 aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg tatgagatga
     2641 aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg aatcaagcga
     2701 ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg atcgtaaatg
     2761 aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc ttgaagtcat
     2821 caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg agatgagtta
     2881 gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa aaatacagat
     2941 gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg atcatattgc
     3001 taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga tacatcacaa
     3061 tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat atgatctatt
     3121 agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg cagagttaaa
     3181 aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga taatgcaacg
     3241 aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac aattaataga
     3301 agaagattta gagaccacag ttcagagatt agcggaacga atccattttg caaattatca
     3361 aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa tgaaattttt
     3421 ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga agaccatgat
     3481 ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag aggcaaaaga
     3541 aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga aatgcgttga
     3601 tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa tagattttat
     3661 tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag atcatgaaca
     3721 gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata acaaggtgtt
     3781 tcctaatgcg tgaaaagctc aaagaaatca gagttttttg gataattaac aaagaatgta
     3841 tcatcttata tactcttgga tttcttagca tgatcttgtt ttggtggtta tatttaaaac
     3901 agtaattgga gagggtcaag aggattttta tcaagataag gatgtggaaa ctgcatgtgg
     3961 aataatagat taaaaacagg attgttatta atagttattt cctgcgctat gatgattggt
     4021 atgaggatac aaagggaaca atcctatttt gaagtttctg ctaacaatgt aatagaaaga
     4081 tgccattatg gtcaacattt ttggactggg gaagtcagag aaaatataga tcgtgaatat
     4141 gttcaaagaa ttgtttggga tgcatattct atcaaagatt atcctaaaag tttaacgagt
     4201 agattatttt acagtgaaaa agataatcaa aaactctcag atttgatgat gaaaaaagta
     4261 tgactattat tacgaaagag aaacagagtg ctatattcga ggggaaaaag cctacaacaa
     4321 aagtgactat tggggtgaca aggcgaagcg attaactgca aattttagag tgtatgaatc
     4381 agagtttata aacttgacct ttcttaatag tgtttttatt gattatgtaa ttactacgaa
     4441 acgcattgga agttattcaa gtacctacgc atccttagtt ccaatgttaa actcgatgag
     4501 ccgtttctta aaagaaagag aaaagaaaga gctacagtat atcaaagaaa tcatgccgga
     4561 ttttgaaatg cacgaatggc aaattgcttt atcagattgg aaattggcga ataaggttcg
     4621 tagaataaca ccttttcaag ccaagcgctt tttgagagct atgaaatttg tgcaataaaa
     4681 aaagccacca gacaagttga tggagactaa cacaattacc aacagttgta attgtgttag
     4741 cttacaaatt aattatggtg tttagtcaat cccaaattgt gaaatggcac caataattgg
     4801 aaaggaaaaa tgaaatgtct gaagatttac gagagctgaa gttacgaaag aagcagtatg
     4861 atcagtattt ttccggtttg ttgaataaac atattgaaga aggacttgat catgattttg
     4921 gaaaagatga atattgttta tattgcgaac gttacaatga aaaaatgtct gaatatctac
     4981 agctgaaaaa agagatacag aaattagaga aaaaagtaca tgcttggaag tataaagatt
     5041 ccatgtctgg ttatgttgaa tctcaaaata agccaagaaa aaagaagcaa gcgtatgttc
     5101 taaagatggt aaatggggaa gagttaattg tattagcgta cagtgaagaa gaggttcgag
     5161 aactcgttga aacacctgtc aggaaaaagg ttgaaacaat aaatgagata gacgacagcc
     5221 aatacaatag catctatatt gaaacaagtc atggtggata taagaaatta gcaactttcc
     5281 ttagaagttc taaagttggg ataatttcta aaaaagacat aagtgctgtt accgatagtt
     5341 tttgtacttt atctgttcct aaagcttatt tccaatacga tagtaaaaga tttgaacgct
     5401 tagagaaatt ctgtagaccg tatactgtat tgaaaactaa aaaatttaat aaaactaaaa
     5461 gagtttctat acagttaccg actggtcaaa tctcattagg tgaaaaagac tatctgataa
     5521 aagtaggaaa agagttaatt ataaaagtac cggaacactt atttaaagaa aattttgaga
     5581 tacgtgaaac cttggtatgg aaagaagaaa aatagtttgg aagacataga atggtataag
     5641 gggaaattga aatggcagta aataaaatat tgcaggtact aatcatagac gaatttggcg
     5701 gaggagaatg gtttgatgtg cagtccatag actttgaaga agaagtcgta gttatagaaa
     5761 atatttacgg cgaagtatat tcatattcgc ttgaaggtga gaatattgag gatttacggt
     5821 tgattggtga gtagaaaaaa acagaaatat caaaatggag ataaaaatta atagcatgta
     5881 aagatagtca tgcttgggtt aaaaatgtta aaattcaaaa aaatgagtca gaagggataa
     5941 aagtatgaaa agagaggaat acaaacaaag attaaacgaa ctgcttgaag aggacgaaac
     6001 attaactcat ggttcgcctg atgaaattct atacatgatt gataatatgg tgatctttgg
     6061 tggatatgaa cttggtaata ggtcagttga tcataatatt ttagaatttg atgatgtatc
     6121 atgggaagaa attcttgatt ggggaatttt ggcggtacct gaaactaaaa cctatattag
     6181 tgacactatg gttccttttt ttgaggaatt ggattataaa agacttccca aaaatgaaaa
     6241 tcatatactg ggaggaaact aacaatgtta agtgacttat ttattttact gatctatcta
     6301 ggtgtaagtg aaattattgc gtattttttg tttgtcattg ctaattatgg tatttccagt
     6361 gcaatcgttc aaatgtttag attaccagta agtgaactaa aaaaatgatg agattatcag
     6421 catatctttt aatgtttttg aatgtggtca cgttaatctt tgtagtaatc ggtgtgactt
     6481 tcgatttaat ggaatagtgg gaaatctata atggaaaaaa gagacacgaa tgaacatatt
     6541 ttttgaatca atattgttga tggtttttgc gatcagcgct taccagaaat ctaaaaaaga
     6601 gtaatgattg tttaatcatc actctttttt tgtagggaaa ttgaaatatg tggagcttgt
     6661 attggtttag ttaggattgc ttggctataa ggagctagtt ctttatcaaa accatcaaag
     6721 taggttttca gtagcgattt aactgatcgc tcatcttgtt tgatttggat aatttcagca
     6781 tccaaatcgg ttgtgagatc aggagctttt gatgcgatta cctgattttt tttatcttcc
     6841 aactctctta ggctgtttaa caagttgtaa aaagaatcat tttgattgtt cattccaacg
     6901 tgagttgcaa ttttaacaga agtttcgtcc gtattgtcat caaaagaact cattggaata
     6961 tcaagtagta acgaggatgt ttcttttact gactttttct ttacagcatt ttttgttttt
     7021 ccatcttttt tgctctttac tcgagatgtt tttggagcta gtttaggtaa atcacttgtt
     7081 ttatcagtca tttattagct tttcctttct attaatttat gttattatat tatcataata
     7141 acataaaaaa gtacactttt tgggggcgta gttattgaaa aaatctaaga gaaagattgc
     7201 cacattgctt atgttaatgg tgcaatttac agggattgca atgagtgtaa tcacaataaa
     7261 tccgcaagtg atacatgctg atgggatagc tacttatgat gcacaattta actgtactac
     7321 tgaaggtagt gggtcaggaa cagatagcga tggaaacgat actggaagtg gcgctgtaac
     7381 aggtgatgta gccaaaaata ttgaagatat ttacaaatac atgaatggag aatatggctt
     7441 atcaggtgct agtattgcag gtattttagg taactggaaa caagaatcag gtattgatcc
     7501 tcgagcagta gaagggtatt acggagatgg acagagagaa gttgacttca tcaaacaatt
     7561 tactgatggt ctgatgggta ttggctatgg tcaatggaca gctgggcgac atacagcatt
     7621 agttgattgg tctaagaaaa ataatggcgg ttcttggtgg actacagaag cccaattgga
     7681 ctttatgttc aaaggtgatg ggggattcgt aggaatatta aaagattatg ctttaaatgc
     7741 ttctgatgat gtcgtagaaa atgcagtaac ttttcataaa aactgggaaa tttcagctga
     7801 ttccgaatca gctgtaatag ctgtacgtgg tgaaaatgca aaagaaatct ggaagtacat
     7861 gaaaagcaag ggtatggacg gtgcaaaaga tgaaagtaag atcaataaaa tatccggagg
     7921 ttcaagtacc ggagatggat cgaataattc atcaagttca gcatcaggaa cagtagaaga
     7981 tgcctgtgca actacggatg acgctaattc tggttctgga tcaattggga aagaaggaaa
     8041 aattaacggt aaaaaaggtc aagtaatagc gaatatgact tatgaagaag ctttgaaaaa
     8101 atatgggaaa gcgatcacgc taccaaaatt caaagaacca gattggtcaa cttcaccttt
     8161 taacggaata tatcaaggtc agtgtacaga gttgacgtgg gcttatatga gccaattata
     8221 cgatgggaat caaccgacta ctggaaatgg tggttatgta tgggaaagct acaaatcggc
     8281 aggagcaaaa ataacgaaca aaccaatcgt aggttatggg tttagtgctt cggatggatg
     8341 gttatgggct gttggagatg cagggcatac tggtgtagtt atagcggtgt ttgaagatgg
     8401 atcatttcta tgtgctaact ttaatgttcc accaacccaa gctccaacaa ggggagtttc
     8461 ggtaacgttg attgatggag tggacggatc agataatgtg catttcttta gcggtgtagg
     8521 aaaagctaaa tttgaaatcg aaaaataaat tggaatgaag gacgatatag atgaatagta
     8581 aagtgaaaga tacaattatc atttctttgc tttctggtat gttattagca ctgcttgttg
     8641 gaatcctatt cttcaataag cagaatcagg acatagaagc tgaaaaacac agtgtgcata
     8701 ttgaagataa gctgacattc tttaaagagg ttaaaccaaa tgaggtggat ggattacgga
     8761 aagatttaga aaatgatatt caagaaatgt tgaatggatc agttcaaaat aagtttgcag
     8821 atcaatcagg tgaagaattt ttgagaaaac tatttattgt aacaggtaaa ccaccaatta
     8881 ctgataagag tagtgagaaa gaaaaaatcg agtactataa gccttttact tttaagctaa
     8941 caaactttgg aattaagaca tctagtagtg ggttgtatga agcattgacc gattttgaag
     9001 ttcaatacaa tggaaaaaaa gtgtatgata ccacattaac catcacacta aataatgata
     9061 agaaagtgac tgggggtgct ttttatgcaa aatcatgaaa tcactactgc taaaaaggcg
     9121 atcggaatac tttccataat tgtgatcctt tgttgtgtgg tactatttca aaagaaaaat
     9181 gcttatgata ataatgtcaa agaattggaa gaccttaaaa gtaaacaaat cgaagtagtc
     9241 aagaataacc agaaagtgaa agctatgcag gaagctcaat acaaagaatt gggaaatgct
     9301 gaaattaagg aacaaagtag gaaattcttt aatttatttt acaactggaa ttcatgggaa
     9361 aaatatacga agaatatgaa ggaaatacaa aaagaatttc ctcagatagt aaagtctagt
     9421 cctgttgata tttctggcac gatggtaggc actggtaata gtccaatatc tagctatact
     9481 gctgattact atacaacaaa tcaaaaaggt aaaatggttg aattgattac acagacaaga
     9541 aatactccaa caacagcaac aacaacaatt tggcgtggag taacaagcat aggagaaaat
     9601 caattgtttt atttggagga tttagatagc tatgtaagag cagagtaaaa taaaaaaaga
     9661 gctaacggta ccaacgttag ctctttgtgc tgacgatgat aacaaaacct accgaaaaag
     9721 gattgtttca tcacaaacac aaacagaaaa gaattgataa aaaacccgaa cctcagaatt
     9781 tttattaatc aattcattta gattataata aattgagctg aacattacaa tacgtatatg
     9841 ttgtaaaacg taaaaaaagt caaaagtaaa tactttctta aaaagtcaaa taaaactatt
     9901 aaagacaata tatatggttt atttgacata agaaaatggt ctagatgata attgtaatat
     9961 agaattgtag acttaaataa agcataaaat accttttatg ataaggtata gctcttaatt
    10021 ttaagcagtt ttagatataa gattgaaagt aaagtggggg agaataataa tggcagatca
    10081 taaattagaa aagttattag aagaaacgtg gaatccaaaa gaatttagtg aattttttat
    10141 ggagaatttt gaaactgact tagcagtgat tgttaaagat gctcttagag agcaagggta
    10201 tcctgaaact gcaaactata taaacattaa ttttactttg tatactgaaa ataaaggaac
    10261 ttgggatttt tgggctactt tagcaaataa ggagttaagt gataaatcag acacaggaat
    10321 tcgtaacttt tttgaatcaa acagagatga ttatatgtat gctaacaatc aaaataaatt
    10381 gaactttaga gtagagtttg atgaaacacc tgaagaattt atagaaagac aaccgcctaa
    10441 agaaaatgta gcaaaggtat tggaagatcg ttggaattca gatgaaatag tttcaacaat
    10501 tagcgaacta ggcggacaat atgaaccgct agtagaagcg gttagagaag agctaagact
    10561 gaataagttt cctgatgttc aaaatattga tgtttctcaa attgaaataa acgtcaagat
    10621 cacgaataaa cttgattatg gtagttgggc agatatagca ttagaaaaat acatctactc
    10681 aacgttaaaa gagtttatcg aaaatagaat ggatatcatg tatctacagc atccacagta
    10741 tttaaacttt ggtgttgaaa tagctacacc tcttgaagaa tggaaaatgg agcaagggct
    10801 tgactagcct ttgctccatt tttttatttt acaaatgaat acagttcgtc gtttttacca
    10861 agaatgattg tttgcccaac tttgagattg tcaggtgatc cttcagcggt ttgtaaggag
    10921 aacgttccat tgaacttatc ggataccgtt ataggtagct ttaattctga tccttcctca
    10981 tctttgagaa ttgctgtttt tcgttcatca aaataaacag tctttattaa ataatttttc
    11041 gtagtgatct cagcagattt gtcttttttt gtagtagtac cagtttcttt agaattagag
    11101 gtattttcta cgactattcc atctttggca tttgagcttg tgtttatttg attgaatatt
    11161 ttcagcgaca agataacgct aagaatagtc aatatagaaa caagtgtaat taatactgat
    11221 aatttccttt ttttgttcat tttttcttaa catccttttt tgttttgatt agttagatat
    11281 tccttatcta acaagccttt ttaggctatt aaatgaagat aaaccccaaa aaatattgca
    11341 attatgacag tgcaatggta ctattattat acaataaatt ttgcgaaagg ggtaggtcta
    11401 aatcgcaaac aaaaagaaat tgaacctaga caaatacaac ttgtataccg caagggaagc
    11461 aagtttgatt ttgggtaaag gtagaaatta tgttacaaac ataatcaact attatccaaa
    11521 tcgtttgcct aaaggttcgt tcaagaaagt gggtgacaaa acctatatta taacggaaga
    11581 aggaatagaa gcattgcgat cacaaacaga aaataaaaac aaaaagaagg aatgaatatg
    11641 aagagaaatt cgcttggtat ggttttattg gcatcagctg tacttgcggg gttatcagta
    11701 ccagtaacct cacatgctga tgaatggact gcaaacacac cggatcaaat ccaaattaca
    11761 acaggacaat caagttatgc gttcaaaaaa ggagatacta tttggtcaac tgtagagaag
    11821 cttaaaagct taggttacaa gactactgtt gctgagtttg ccgaattgaa caatgttgat
    11881 ttggcaaaag gagaacaacg gactatcccg attggacgaa ctttcaacat cagtaaagat
    11941 acattaacag ttaaggaagc agatggcgaa gtagttagtc aaactgttca aaaagataaa
    12001 cctgatccta ttgaaaatac ggagcaagag aacaaaaaat ccgagactac aaaagaatcg
    12061 gaaccaacag caaacacttc tgataagaca gaaacaagtg cttcaagtgt tgatggagca
    12121 agttcatcta atacaaataa ttctacaagt gagaaagaag catcacaggg aacaagtgaa
    12181 agtagcacta cggaagctga tactggaaca tcaacaaata actcacaatc aacatcaaat
    12241 gatgcaaatg aatcaaccag tacatcttct agtgatcaat cacaaacagg ttcgagtgat
    12301 gaaactgaca gttctacttc accaagtaaa cccgaacaac caactaatgt tgtgctaggc
    12361 gttccattta ttagtcaagg taacacaatg ctttgcgaag gaacatcttt gctagaagcg
    12421 ttacattata aaggtgttgc aacaaatcaa gatttaatga cttttgttaa atcaatgcca
    12481 ctatctcctg acaataatcc aaataatggt ttttctggtg aatggagaca caatgtggat
    12541 ggaacttatc aaggtatgaa tccggctcct gtagtttcat ggggtgttgc taatggcgga
    12601 aatgttgtag atatttctgg ttcatcggta caaggattaa aagatgagat tgtaaatgga
    12661 aatcctgtag tagcgtgggt aacttatgcc tttgaacctg ctcaacataa accaatgttt
    12721 tggggcgatg ctatttggaa tcgacatgta gtagccgttg atggatttaa agacggtttt
    12781 taccatgttg tcgatcctgt gtttggggca agttggatta atgcaggatc atttgaaaca
    12841 gcatataacg tatctcaatt agcggtttcg gtacgttaat aaaagatatc gtttatatac
    12901 taagtagtta tatagtcttt atttgtcctt gcttaactac ttagaaaact tgttgctcat
    12961 ggtgggtaga actaaaaggg gtgatgctat gaatactaga gcgttgaaac agcttgcttt
    13021 caacgaaaaa aatgattaat aggtgtggtg attttaactt aggaatgggc gcgaaggtca
    13081 ttaccacacc gacatatcgt ggctcatttt cttcgcctaa ctggtataaa gaaggagtaa
    13141 cgagagatga agcatgaaaa aacgataatt ttaaaatcaa aaataattta ttgttttgcg
    13201 actttagtaa tgatgttatc tacgcttata atgccttttt cagcaataag agctgaagca
    13261 gatgtcacaa aaccgacaag tgcaaaattt atagaaactg gcgtaaaaac agacggttat
    13321 attagggtta atatgccgaa tcatccaaat gaatggatga tttcaagtca atttaaagat
    13381 tctcatggaa atattggata ttgtatggat tcagaattgc catccccaac aggaagtgga
    13441 gccggaagct tgaagtacaa gggagctggc agtgatgagt tctatcgtat gtttaaaggt
    13501 ggttttccat caaaaacagc aaaggaactg ggtgcaggaa atgatacaga agcatggtat
    13561 gcaacacaat tagtttcatg ggttttagcc ggaaacttta aagtttcaca gattgtatgg
    13621 tcacatccaa accataccgc cgcagaaaca gctcgtgtaa aaaaagcatt tgaaaaaatt
    13681 tatgactatg ctaagaatgg aaaagatacg cccaacactg agttttctat cacagcttct
    13741 aagacagctg acgaaggaaa atatcacact ttcacatata aaacagcatc aaataagact
    13801 ggtaatgcga agctcacttt tacatcagca aaaccagctg gtatgaagat ttatgatgca
    13861 gatggtaaag aaattactaa caacactgtg aaattaaatt caagttttac tattaaagta
    13921 cctgtgacta caccaagtgg tactttgtca tttaaaggta ctgctaatgt tagcacaacc
    13981 aatcctttta cttttgatgg acgaggtgtg tatcaagatg ccgtagttat gatcactact
    14041 tcagaaacaa aggactctaa atctttgagt gctaaatgga caagagctgt tggtcaggta
    14101 acagttaaga aagttgatca agatggaaat gctttaaaag gagcggagtt tactctaact
    14161 gacaataaca atgctaaatc tgttaagaca accggagatg atgggttagc tacgtttgat
    14221 ttgcaacaag gcttaacata cagcttagaa gaaacaaaag ttcctgaagg ttatcaaggt
    14281 tcatttaaaa aggatggtat tactgttaat accgatggac aagaatttag ttacactgct
    14341 acgaacacaa ttaaaaaagg taaagtaaaa gttaaaaagg ttgaccaaga tggaaaacca
    14401 ttagctaatg ctgaatttac cttgacggat gattcatccc aaacacaagc agaaaaaaca
    14461 gatgaaaacg gacttgcaag cttcgatatt gtagcaaaca agacctacag ccttgaagag
    14521 accggagtac caactggtta tacaggcgta ttcaagcaag aaggaattac cgttaaagat
    14581 gatggacaaa cttttgaata tacagctaca aacactttga aaaaaggttt agtaaaagtt
    14641 cataaagtcg atcaagatgg aaaacctttg gcaaatgcag agttcacttt aaccgatgaa
    14701 aaaggtgcaa cacaagtaga aaaaacagat gaaaatggac ttgcaagctt caatattgta
    14761 gcaaacaaga cgtatagcct agaagaaacc ggagtaccag taggttacac aggtgcattt
    14821 aagcaagagg gtatcaccgt taaagacgat gggcaaactt ttgaatatac agctacaaat
    14881 aatttgaaaa aaggtgtagt aaaagttcat aaagtcgatc aagatggaaa acctttagca
    14941 gatgcagaat tcaccttaac ggatgaaaaa ggtgtaaaag aagttaaaaa gacggatgca
    15001 actggtctgg ctacgttcaa cattattgcg aacaaaactt ataacttaga agaaacaaaa
    15061 gtaccaacag gttacacagg cgcattcaaa caagaaggaa ttaccgtcaa agatgatgga
    15121 caagtctttg aatatactgc taaaaacact ttgaaaaaag gtgtgatcaa agttcataaa
    15181 gtggatgatc aagggaaagc attagcaaac gcagaattca ctttgacaga cgacaaaggt
    15241 gtaaaagttg tacaaaaaac ggacagcaat ggcttggcaa catttaatat tgttgccaac
    15301 aaaacgtaca aaatcgaaga aacacaaaac cctaatggtt atacagggaa atggtctaaa
    15361 gaaggcatca ccgttgctga cgatggacaa acctttgaat atacagtaat taatcacctt
    15421 gtacctgaaa cagtaagaac aggcggaatc ggtacatcag ccttccttct tgtatttcta
    15481 attgcaggat tgagtatggt gctactgtat tatcgagaac agcacaaagc aaaataaaaa
    15541 agtgttaatg gaaaagaagt aaatatgaaa aaagaaggct gacgtatgaa atcaaaaaaa
    15601 attttttcaa atttaatgct atttggtact ttgctaggag tgggattggg cgcaagctca
    15661 atctcttcta gccttggact aggggggcaa gtagcatatg cacaacaaaa ctataattat
    15721 caaggaactg gaacgattca tatccataaa gtaacaaata ctaatgggga tgggaaccct
    15781 gtctcagata ttgataacaa agggaaagaa atagctttac caaatggtgt aaaacctctt
    15841 gaaggcgttg agttctctta tgtgaaactt actaaagatg aagctaaatc cttgggatgg
    15901 acggatagtg atactacaaa aggtcaacat attgatgatg ctaaagcacg attattacta
    15961 aacaaaaaat acagtgaagt aagtagaatg aagactgatt cacaagggaa tatagacata
    16021 cctatacaag taaatacaga cgatttaaca gataacttgt atttgattgt tgaaacagga
    16081 acagtcaaag gtgtagttga agaagcgtat cctatgattg ttaacgtacc tgaactaaac
    16141 ggatcagatg atcaatactt taattatgat gtacatgtct atcctaaaaa ctatttccaa
    16201 ttaaactcag cagagcttga taaaaaagac gaagatggaa aggctcttgc cggaactgtt
    16261 tttgaattgc gaaaagatga tggatctgat caggttgttg gaacttataa aacagacagc
    16321 acaggtaaat taactgtaga tggtttggaa ttaggtgact attatttcct agaaaccgat
    16381 ttgggaacag atgctaataa aacaacctat ttactagatg gtaaaaaatt caaatttagc
    16441 gttaaacgtg gggagactgc taaagtagtt cttgatgtta ccaactacaa acgacctgta
    16501 attactaagg taattgataa agaaaccgtt gatactggcg aagatgttca atatacattg
    16561 aaaccatcaa ttcctggtaa tattggtaat tacgaaaaat atgttgtcaa agatgttcta
    16621 ggtgcagagt taacttatga ttcatcagaa gtgatcgaag gaatgacttt agatactgat
    16681 tacagtatcg aacgtgacgg acaaacgaca aactatgtct ttactgaatc aggtatggat
    16741 aagcttcaga aacaatttga tgcaggggat cgcttcttta caattttgat gaacgctcat
    16801 actaatagta aagctaaaat ggatacacca cttaagaatg atgtttcaac gatctacaac
    16861 aatggatatg aacccgaaga cgaaacacca gttgatcacg cttatacaat cattggtgat
    16921 caccaattca ttaaaacaga tggagataca agcaaagcat tgagtggagc aaaatttgta
    16981 gtgaagaaca aaattgatcc gaataaagtt acttacctaa aacaagatcc aacaacgaaa
    17041 tctgttactt ggattgcaga taaatctaaa gctactgaat tcgtttccgg atcagatggt
    17101 aagttccaaa ttgaaggctt gcaatatacc tatgatgtag attttgataa acaaggtaat
    17161 ccaacaatca cagataaaaa aatcaatgat tatgctcttg aagaagttgt tgctccttca
    17221 ggatatacac caactagaaa tgatgttcca tttgtggtat cgaatgatga tgcttctagc
    17281 gaagtaacag acatcaagaa ccttaaaaca cctgaaacag ttcgtacagg tggaattgga
    17341 acagtagtat ttctagtggt tgcaggtgtg gcaggaacag ggctatatgt tgtctacaaa
    17401 cgagaacaac acaaagctta atgaaataaa attccctaga gtgtttttgg ctctagggtt
    17461 tctttttaaa aagtgtgtga aaagtaggta tgagcatgaa aaagaagaat atcaagttga
    17521 tcatcttaaa aattgggatg ttgctatgtt taggagtagg tctatatcct gtaattactc
    17581 aggcttatac ctactaccaa actcaacaaa cactaaactc ctataaggaa aaatatgatg
    17641 aagcttctga aaaggagaat gaagaagtct tcaagagata ttcagaagtt aataggaaac
    17701 gaaaagacac agaacaaacc tcagtcacga ttcctgaaga taataacaat attccgtcaa
    17761 atggaaatct tcagaaggat aaattaggtg aattagtggg tgaaattctt ataccaaaga
    17821 ttaatgtgga tttgccaatt tatgatggtg taaacgattt ccaactatca gcaggagcag
    17881 gaatattaac cggaactgat tatccaactg gtgggattgg aaaccattct gttatatcag
    17941 ctcattcagg cattcctaat aaaaaatatt ttacagattt agagttgctc aaacttggtg
    18001 ataaattttt tattgatatt tcagggaaaa aacttgctta tcaagtagat cggataaaaa
    18061 caattctacc aaccgaattt gaggatttgc ttcctgtggc aggaaaagat tatgtaacac
    18121 ttatgacatg tacacctgtt ggagataatt ctcataggtt actcgttaga ggggttagca
    18181 taccttacca aaaaagtatt gttgagaaag aaaagaagaa agaagattta aaggtaaagg
    18241 cgcagatgaa ctggtatcct atcgtagttg taggggtttt attaataata gctgtgatag
    18301 tcattattaa aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa taaaatacta
    18361 ctttttttga ctcttatttg cactttggtt ttaacaattt atcctgcaaa tcagctctat
    18421 catcaattca aaacaaaaga gaatgtggcg gaaatccaag agaaaattga gaaaaaacaa
    18481 actccttcaa aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa cagcggtaac
    18541 caaggattag acaatactca gaaaacagat aaaggagtag acaatactca ggaaacagac
    18601 ggtggatcat ttgatattcg tgaagctgaa ggaaaggtca atccatacac aggagatgtt
    18661 ttttcaaaaa atcaaacgaa agattttgaa ccatctacct attcaggagc ggttggttat
    18721 gtctatattc caaaaataaa tgtgctagag cttttgtttc taggcgcttc acaggagcat
    18781 ttatatagag gtgtggcaca aatgagtgct actagtctac ctttatcagg taaaggtcat
    18841 caaacagtaa tagcaggtca tcgtggcggt tatacgtcac cacaatttct ttatgttgat
    18901 caattagtag ccggagacca catttatata gattatcttg gtgaaaaagc agtttatacg
    18961 gttttccgaa cagacgttat tagaaattac ggtcagaatt caacactgac tgatattcct
    19021 gatttagata tgttaacact ttatacttgt catccatatc ctcagaacta cgatcgttta
    19081 ttagttagaa gtctaagaga tgaaaaagca aaatacatca cgccacaagt aacagttggc
    19141 aataccgcat acgctgatgc cattgatagc gagtccgttt cagataagac agaaactgtt
    19201 acatcttcta atgatttatt tgaagatgtt gatcgtgcca acaaggaatt agatggaaat
    19261 aatgatctta atattccgga acaaggtagc gtagagatgc aagacagaac gtttgctgaa
    19321 aaggcaagtt caatatttac aggaattacc aataaacatc agaaaatgct tttagtcatt
    19381 tggactgtaa taggagcatt agcaagtcta aaggttctta ttcttttaat caaagagttt
    19441 cgaaaagcat aatgaagatt ataaaaattg atagagtaca gtaaaaaggg attgatacta
    19501 ttattgatct cttttttatt ttgaatagag ctagattcac ttttaaataa aatcaaaact
    19561 gatcttaatt ctaaagaaaa agtcaagaaa aaaatacaat aaattttttt atcagaatat
    19621 ttatcagaat attattagaa aagataaact caaaagctta atttaatagg gttattggtt
    19681 gtatttagaa ataatgtatg gaaaagctat aaagcaatag ataagatgta gaaaatctta
    19741 tttgtgaatg aaaaacaagt catcgtaaaa tattaatagg aggtgtagta atggtgggag
    19801 tatcaaaaac agctttagac tcattagcta aactatttag tagcaggttt gatggtgcag
    19861 aatttgttga atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa acaatgaaaa
    19921 aagactattt gaatgaaaat gactttccga catggacgaa tattgaagat gctacatggg
    19981 atgtagtatg tacgatatat tctccttggg taacgattgc tgattttgtt ttagataaaa
    20041 ttggagggga tacataccct gatgccaaat ctcttgctac aagtgacttt aaaacgttct
    20101 atggttatct aataggtcta ataggcaatg gaatagattt gaaagttacg ccaaaacaaa
    20161 cacctgaaga aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca aatgacagag
    20221 aaaaagtaaa attattggaa ggtaaattat ctgaagaaga acttggaaaa atggtgcaag
    20281 aaatcaatga agacagcaac tattttcctg aattgaaggt atatcaaaat gacgaagatt
    20341 tctttaatga aatgtttagt aatagtccat gggaagcagt aagtaaaaca aatttaggag
    20401 aatggaactt tcaagatgat ctagttgcat ttagcaatga tgggtattta cgatcatatt
    20461 cagaaagtga gtatagagct gaattaaaaa cgtatgaaac ggatattctg atgagctatt
    20521 taaatctatt agagagtggt cagggagagt taagagataa agttgaagaa ctattaaaag
    20581 aagagctata gggattattt caacataaac acaagcagaa aactaccaaa caaaacgtac
    20641 catgcaaaaa gtcggttaaa ccggcttttt gctctattat gagaggagat caaaccatga
    20701 gtgcaacaac cgatatggct ttagaagcag taataaaaga taatacagga aatgtcgaaa
    20761 atgtagaaga ctttagcgat attgcaatca atctttcagc atggatttca gctgtaggag
    20821 aagaaaatat cgaatttgag atcaagaata tggatgactt aaaagcaaaa tttcctttta
    20881 ttgcatcgaa actaagtcaa gttgccgagg ataaatcgtg gaatgaatta gttagttttt
    20941 ccgaaaaaat ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa atgaataatc
    21001 aatacaagaa aattgacgct ttcgtcaaat cacgtataga aggaacagct tttgagtttg
    21061 aaggtataga taccgcagat aattggttaa ataaagaatc ctttattaaa agtatagcgg
    21121 atgatcttga aaaacaatgg tctaaggaag actatgcaga gtacctaaaa acagaaagag
    21181 atacagaagt gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt gatcccaata
    21241 tcgtaaaaca agaagactat gaagtggtta ttgataaagt tcctactgat tttgaggaag
    21301 ttgtaaagta tatagttgaa gaagcattat cgcaatactc ttcagatgag gaagtggacg
    21361 aagaaactgt tcgtaccaga gtttggcaac tggtttttga tgacaaagat tataaatatt
    21421 tgagtgatca aaactcggtt aattggagtg tacgattatt aattgatcaa aatgatattg
    21481 aataaaatag agctgaaaag ctctattcag attgtagaca aaaaggagtt ggtaattaag
    21541 ccactccttt ttgttagtct ttgcataatt ttgaaataaa aagccatagg cttttttgtt
    21601 ttccatagcc atgtggctag tttttttaaa ttcatggcag caaacgtaag catcgtgtgc
    21661 gtggcgactt tttcaagacc tcgatatttc gtccaacgca taccatgctt ttcttttgca
    21721 tctgcaaata ttcgttcaat ggtttgtttt ctctttttgt aaatttctct attcaacgcc
    21781 gtatggcgtt ggtgttcaag ttcatccata agatcagccc aaatatgtcg agtcactatt
    21841 ttttgatgtt ttgtactcaa agtacaattt tcaagtaatg gacaactcac acagaatttt
    21901 ggatttgatt tataggttcg ataaccatca cgagtgatag ttgaaaataa caacggttga
    21961 ttattgggac ataaatagca gtcaaaatag gcatcataaa caaagtcttt tttacgaaac
    22021 attcctttca ctccatgagg tgcagtatat ggaagaatcg ggtagagttt caagtgtgac
    22081 agaaaatgaa caaatttagg cgtcttatat cctgcatcag caaccacacg ctttatattt
    22141 ggaaaagctc ttttcgactg tttaaccaga tcgattgcga cttggctgtc atgcacattt
    22201 cctggtgtga ttaatgtaga taagataaaa ccgttatctt cacaggaagt atgtgcagaa
    22261 taagcaaatt gtttttctcg ttcaccttta acatagtaac cactttctgg atctgaatga
    22321 cttatttttt gaagactcaa ctcactttct gtgtcccatg taaaaggacg ttttcctaca
    22381 gcaatacgtt ctttatttat ttcatcttct aattcatctt gataagcttt tgcttgaccg
    22441 taggtcatct ctttcgtgaa cttatgttta ttggcatttg ctttgatatg agtagaatca
    22501 atataaaggt tgtcttccgt aacgaagcca gcttttacag cttgttctag aatatgagag
    22561 aaaatatctt caaataaagt tgtttctcta aatcttcgaa catagttttt accaaaagta
    22621 gagaagtgtg ggataggatc ctcgaatgaa taacctaaaa accaacggta agcaacattt
    22681 gtttcaacgt ctttaattgt ctgtctcatc gaacggatac caaataaata ttggataaat
    22741 actaatttaa ttaagaccac tggatcaata ctgggacgac cataggttga atacgtagat
    22801 tctacaatgg gataaatgaa atcaaactga atcgcagcat caatttttcg aactaagtga
    22861 tctttaggta caagatcatc aattgttaac atagcaaatt gatttctacc ttcaatcgat
    22921 tgttttctca tcattagaat cactcccaga atagtttata caattagtat aaaacaaaaa
    22981 actgcaaaca acactccaaa tttaggagtt tgtctacagt ctgaatagag ctgaaaagct
    23041 ctattttttt tgataaaaaa actagctatt ttattaaaaa taatttggtt caaaatttat
    23101 ttttttaact tgataatttt ttgccatcct tttttaaatt ggctatatat caaggtttat
    23161 agtgggaata aataaaaata catttgacaa aatttacaat atagtttatt atatatttat
    23221 cttattatga taacacaata aaaggaatgg tggtatgaaa aagaagaagt ttagccgatc
    23281 gttgttagga attttggttt tagttgcggt tggtcttagt ggcaagggtt actttcagaa
    23341 taacagtact caggaacatg attttcagat caaaagtagt gttcaggtag gtaataaagc
    23401 agacagtgaa tcaaaagaat ttttagggat acgttatgat ccaaacaaat atccaaataa
    23461 tatcgtaaac attaatgatg ggaaatatac actgacaaaa gaagaccatg aagcattatc
    23521 agaaaaaggc agtcaaacct tttgggtaaa ttatgcagat ttagatcaac ttggaagagc
    23581 aggagatgtt gaagcgttga tcacctatga tgctgtaaat tctcactcat ctaaaaatgt
    23641 ggaacgtcca tctttcaaaa gttctgtcca tattgcagga gaatacaagg atggagtatt
    23701 tgatcaagag agagatactt gggtcggcaa agattcaaac aatgaaatca ctcagctaga
    23761 aggctataaa ggttatctct ataacaaaag tcattcatta gcatggtcgc taggaggaga
    23821 tatggaggta cacaatttaa cacttggaac gagagcgcaa aatgttggga aaaatgatca
    23881 gaatggaggg atggcttact cagaaacgat tgtacgtgat gcaatctata aaaataagaa
    23941 tgaaaaagta ttgtataaag ttgatccaat ttataaaggg aatgaattag ttcctcgtgg
    24001 ttctcatgtc gaagctttct cagttaacga taatggaaaa acggtaaacc ttaatgttta
    24061 tgtatttaac aatcaaaaag gaatcaacat caactatcaa aatggtactt gggtaagaga
    24121 ttagaaattg agaaggtgaa gaattggaaa caataagcat caatggatta gataaatcag
    24181 ttgcaaatcg aataaaaaaa ggaaatggct actatcttct gaagaagggt aaatattcag
    24241 ttgctagaga gttagaaaca atgagtatcg tgtatgtagc taagaatcat tttgacgtag
    24301 aacaggctat ttttgatcca gattctgaaa tagggaaatg tgttgaatta tcagagaaaa
    24361 ctgttagtac aaaaggggcg atgatcattg agagagaaat gttagacgaa atcagagtaa
    24421 tcattcctaa agatacgcag atgtgtgagg acagcataag tgacgatgaa ccaaaatcag
    24481 aaagaaaata aggagatatt cagatgagcg aaaaatccat tgaattaaag gaatataagc
    24541 gaagcgatgg agaaaaatat tatcctgtag ccagtttttc taaatcagta tatctggttg
    24601 gacgtgcatt ggtagttggt gcaattggca tatatatttt gcattgggta acaaaggttg
    24661 tacaactgct tgtagatacc ttagtaagta ttcttgtctc gttaaatgat gttaacggaa
    24721 cagatgattt aggattaaaa gagatgttta aagagttact caatgcgttt tttcccaatt
    24781 atttaagtta ttcatataat gtacaaagct tttttgcaaa gctacacttg gtagacatct
    24841 ctattttagt attagaagca ggaacggtat tctttgttgg atatttggtt actttgatgt
    24901 tcaggcatca ctatggagaa caaagaccat tttttgatga tatggaaagt agaaggttga
    24961 aaagagaagc tctagaagct atggatgtga agtatgatcc aaacaagatt ataggagatg
    25021 aaacgaacaa caaaaaaaga aaacgaaaca aaacgcaatt agaatccgat gtgctatcaa
    25081 cgcttaaaaa tatcaaaatt tctgttcata caagacgtga tctaagtgtc aatgaagata
    25141 ttcgagaata tatcgtaaaa atcccgcaac caaaagggaa gaaagttaga gaaacagttt
    25201 tacgacaaat tcaagattta gggtcgatcc tgacaagagc aacaagcgga cgagccaaat
    25261 ttggtgaaaa agaagatgaa gtttcaggcg attatttcat tttccgagca tcagtaaata
    25321 ttgaaagaga acgtaaggaa agagtaagta ccaaaaacaa agaaacggaa agagcaacat
    25381 cttcaatcgc agtttcaaat tcacaatttt cttttccaat caatctcttt gccgataatt
    25441 ccaaaaatat ccaaaaccaa accgaaaaag ctagaaaata tgccgaaact caaaagcaat
    25501 ctttagaact ttatctatct tctgctgacg tacctgcaag tctaacagat atgcacgttg
    25561 ggaatacatc tattgaatat ttgtttactt tatctcgtac cagaaaaatt caactaagct
    25621 caatgaaaga agaaattgaa acttacttgc atatatcagg tgtaatcgtg cggatcaacg
    25681 cagggcgtat tcaaattact ttaccattag gagaaaatga tcgtattcca attgatgtac
    25741 catcaatgat caatagcgtg ttttagaaga catcacaaat agaaacagaa aatacaaact
    25801 accagttcac aaacagaaaa gtaccgagaa aagtgaggaa tagaaatggc tatgaaatta
    25861 ttagagaaag tcaaaaagag agccgaaaaa gctgaatcta ttttgctaga taaagaacct
    25921 aatataaagc ttaatggaaa attcagtgtc aatatgttga acacaaaggc taaagaccca
    25981 acagatgcta tttttggaat taagcctgat ggcacaattg ctcatttttg catcagcgaa
    26041 atgccacaca tgttggttgc aggaacgaca ggatcaggga aatcagtatt actgaatgaa
    26101 attcttgtta cagtaatgtg tcactcaacg cctgatgaat taaaaatagg aattgtagac
    26161 ccaaaaaggg ttgaatttgg aagatacaaa aaattgccgt tcatgttggc tgatccaatt
    26221 acagatatgg atgaagctta tgattttttt gaatatcttg tgatcttgat gcatgaacgt
    26281 tacaaactga tggaaaaagc agggttacaa aacatcacgc tatataacga atatgccgaa
    26341 aaaaatggat tagatagatt tccctatgtg atcttattag ttgatgaata ttcacagttg
    26401 gttggaacac ataaagaagt agaaggtttg attgttcaat taggacaaat ggcaagggca
    26461 gcaggtattc atgttatctt agcaacacaa tctcctcgat caacagttgt cactggtatt
    26521 atcaaagcca attttccgtc acgtgtttgc cttatggtag caagtgattt agaaagtagg
    26581 attgtattag atgaaggcgg tggagaaagt ctaagtccaa agggagatat gcttattaag
    26641 cttgttgatg gaagtatggt tagagcgcag ggagcttaca tcagtaacaa agaaattgaa
    26701 acaatcttca atcaccttag aaatactatg ccagaaccag aatatgttga ttaccgagca
    26761 atcgtagctg aaactcgtgg tgagggcggt gaagacgagg attttggtgg aattggtaaa
    26821 gctcctgtaa gaaaatctgc taaaagaaat ttgaacacac ctttagcaaa tccatcatct
    26881 aaagagccat caattgaaaa gaagagtgcg tttgctatta agcttgaaaa gaacaaagaa
    26941 gaaaaagtcg agcagggagc taaaacacta gaaaaactgc aagcagaaaa actagaaaaa
    27001 gttttaaggg cattaggaaa agctgatcct gaaccgcaaa cctcaaacaa cacgaataat
    27061 aaacagccga ttgcatctaa aaccaaacct aaagctaatg atccaaaagg ctcagaaaac
    27121 gtagtgatca caactgataa atctgaaaag aaacaaataa atggaagtac ttcacctatc
    27181 atgcaaaaaa tgttaaaagc aaaaaaacag gtgcataaat gatcatcgtc agttcttttt
    27241 taagatggct tactccatat aaatatacag atgaagtagt tgtgaaacga gcgaagaaaa
    27301 gagataaaaa agcggtacta actcctaaac agatgcttga tcatggcatt ttaggggtga
    27361 aggttttaga agtgaataaa ccgtttgaat caaatagtgc aagagaaaag ttgcttggag
    27421 ctgaagccga tgtcgcatat ttttaccatg acaaaagcaa gcatcaaata gctattaaat
    27481 tcaatccaaa atcggcatgg cggtatcaag caagagatga cttgaaacgt aggaaactta
    27541 gaccaatgct ctatccagaa ggaaaagaag catttgatag aacccacatc gttcccattg
    27601 gttttcatgg tacagaatca gataaccgcc tattagtagg ttggaacagc aatcaaaata
    27661 gagttcagat caataaattt gagaagaaag taaggaaatt caacgatcga aaaacgatcc
    27721 tatggtttgc agatatttgt aaacagccgg atggaacagc aaagtgggaa gcggtgatat
    27781 ttgacgaaaa tggtaaaaaa gtaatgtctg aaacatggca tgataaagct aaatttgttt
    27841 ggcagtagca gtagaggtga aaaagattgc tagaactttt tgaagcaaat agaacaggct
    27901 ttaatcaaaa gttagaagtg ggagaaacta tagaatataa agataaaaac tatattatta
    27961 tcggaatcta taacacgaga attaacagaa taggtgatcc acgtataacc agtgacttgg
    28021 tttgtcaatc tgttaactat tcacctgatc tgacaagcag gtataagaaa tacgtgttat
    28081 tagaatatag acacaagatt actgatgaaa ttggtgtaaa caacacacga aacatattta
    28141 agatcggaac agtgattcct tatagtaaca acgaagaaaa aatattttat cagatttacg
    28201 gaatagaaaa gtttgaatat gaacatgtcg atctgctagt gacgtaccaa atgcgtttaa
    28261 tagaaccttg gtcacaggca gaaattgata aagcagtaaa attgaaccga ttatcaaaat
    28321 ttaacgtatt aggaaatgcc ttttaaaaat cgcaatacac aaacagaaaa ataccaaaaa
    28381 attagcaatg aacgtaggta agaacatgac aataaataat aataacccta aaaaatataa
    28441 tgctcttgtc caatgctata tcgaaaacaa aattacaatg caagaagtcg ctgatgaatt
    28501 aggcaaatca aagagcctag tatcacacga ttttaagcac aatgtttttc cgaacccgat
    28561 tgagctgagg aaaaaatttc aaaagagagt ttatcaagaa ataggtaagt ggattgatga
    28621 agggaatgca attgaccctt taattgaaaa atatcctttt ttaaaaagca aaaataaaat
    28681 tcaaaggtta attcgtcaag gcgatattca agctaaagaa cctgttttga caaccaatga
    28741 tttggctaaa ataaatcttc ataaacaaat tattgaagac tttgaaaaaa atctttcaac
    28801 aactgaaatt ttaaaaaaat acaatattcc gagaggaaag ctaacatcga ctctttttag
    28861 agaatatgga gaatcggcag ttactaaagc gaaggaagta gcgaatgaga gaaaaaaaca
    28921 aaaatagtag tcgataccta gtagcgactg tagtttttgt ttgtgtattg attttcctgt
    28981 ttgatttttt gcagattgga aaaagcataa ttggaaatac attgagtgca aaggaaatgg
    29041 ttgctttagg tggtttgtat gaaggacact tgttcccgaa tatattcatt tctatcgttt
    29101 ctcatattaa cttgtaccat ttaatttcta atttattgtt cttagtagtt cttgaagaag
    29161 cactgaatga tcagtatgag tggttaacat tattgatcgt tttaatcata tcaggatttc
    29221 taggaagctt agttcctttt ctgctacaca gcaacatcgt aagtatgggg cttagtggag
    29281 gtatttttgg atggattgct ttatttgcga ttgcatacat ccgttttgat agcaagaaac
    29341 atttattact aagtgttttt gtattattga taatcggcat tttattggta tctacttttg
    29401 aatcaggcga ggcaaatgtc ttgtcacatg taatcggatt tgtctgtgga atgatcttgg
    29461 gatttattct aaagaacgtt tcattgatca aacaaaagaa ttaaaaggga gtgctaaaaa
    29521 tgccaaaaag aaaagaaaag aaaattccag tgtttttgtc tgtctcagaa atgttatcac
    29581 ttcaaatact gttaagaaaa aagattgaaa caatagacga agaagttaga aataaaaggg
    29641 ctatgtacga acaacgtgaa gcgctaagag aacgaatcca ctattttgat gatatacaca
    29701 ctaaaatcta tgaagcacgt acaaatcacg aagacgaaag taaattagga ttagctcaac
    29761 aacggatact tgaatggctt aatacgaata ctgatcaaga accgttaaga gtgattagta
    29821 atctttttga atttggaacg aagcattggt ttaatgctgt agagaaggat gctgacttac
    29881 aagaagcgtt tgatacgcta agtaaggata gcaaatccat gcgagattta ttgattactt
    29941 atctaaatga gcatgagttt gaacaaacga aaaccaaaaa gaaaaataaa gaatagaaag
    30001 ttgtgatgaa tcatgctatt aaccctgtta ggttatttat gtttaattct tgcttttttt
    30061 ggggcaatcg ctataattat tggtcaattt caaacaaata atccgattat gcgtaacata
    30121 ggcattggaa ttgtgattgt aacctttatt gtgtttaatg ccactaagat ttttggaagt
    30181 aacgataaac aatttggtat atcgggagtt aataatagtt atgctccaga ttctttggga
    30241 agggtagtaa tagatggaac atttgaaaat gtaacgagtt ataaaaatgt ttcgctgaca
    30301 gtgaaggaag atggcaagaa agcatcagac gacgttctcg ctactattaa gccgtctcaa
    30361 tcacaatttg aagtgtttta tacagttaaa gagaatagcc ctgtacacaa gctgacattg
    30421 gtttttaacg ttgatggaga agaatactat aaagatgtaa aaataaataa aacatcaacg
    30481 aaaaagatta tcgaatcagc gaaaaaagaa gtagctacag aagaaagtac aactgaatca
    30541 agttctactg tcgaagcgga actaccagat aaaattgatt ttgatcctga aaaagtcaaa
    30601 tcgtttgaat ctgtttctgg ttctatggct gatcggttta acactgaagt cttcaaagct
    30661 atcaaaactg ttaaattgga tactgaaaag caaatgatta atgttacctt attcaattca
    30721 gttgaatcta tggatcagga taaaaaagtt acggttgcaa gagaactagg aaaaagagta
    30781 attggtttgt atcgtggtac tgctaaacca gattcagacg gtgcagattt gccagttcat
    30841 gtagtttatg aagacggtaa agtttttgga gattctacag caaacgaagc attaacgatt
    30901 aaattggcta aatagccaga aagttataat aaacaaatgg tgtcacacca ataatatata
    30961 ggtgtgacac cttggagatg atagctgatg gtagtaaata aagaaaaagt tagaaagtat
    31021 gtgactttaa aaatagcttc tgttaaatat ttagaaaagc gagctaaaga agaagaaaga
    31081 gcaagaggta agaaagtaac agtttcagag ttgttagaag aattgatcga gaatgatcaa
    31141 acaataagac gtgcatttac taataggtct atcagataac aatataatta ataaaaaaga
    31201 aagagtgatt tggtcttaga ctaaatcact ctttttatat aaagtttctt tacactcatt
    31261 ccgaataatg ttaaaattct ttatatgata tatagttcct tgtcagagga agagataacc
    31321 agcaatattg gagggataat aagtggaaaa caataatgat attagacatt ttagcattgg
    31381 ggaaagagtt acaacagtaa gtgaattatc aaaggattat agagataaag agggaacgat
    31441 tatttctctt aataagaatc gaagaggcgt ttggcaatat gttgttgatt taggtgatgg
    31501 cgttacagtt gagtgtaaag atagtcaact gatcagaaat aatggcagtc attccattga
    31561 aaaagcaatt cttgtatacc caaaagtatc aagtaagatc atgaatgact ggttgcttgg
    31621 aaaatttggt gagtacaata aaaaagaaat cgcagaagtg atcggcaaat cttatccaac
    31681 agtattaggg tatctaaaaa agataacgag taaagaagca aaaattgagg atgttttaac
    31741 agtaagagag cttcaatcat taatcgagta tcggaataaa caaataacaa atcctgagta
    31801 ccttagaaaa gaagatggtc tattgaaaca attggaacaa gtaaataaaa tccggaagat
    31861 gcaaaaggta gctcctttaa gccttgatac ctttaaaaaa atctatttga aagagatcaa
    31921 aaaagactaa tgtggactgt aactttaccg ttccaacctg tatctgcacc aagaccaagt
    31981 gcaagaagga atgctgctga tgaaactaat gacgtagcag agaaaaggat ttcaacctac
    32041 tatgataaaa agtatcttga ttatttgaaa aaaataaaga gctttatcga agatcaagat
    32101 ttattagatg atgaatttta ctcgattgta aatgatccta tgggcgcaat tatggaagtg
    32161 gacttttact accagatacc aaaaagctat aaaaaagtct ataacattat gaagacaaca
    32221 gcgccagatt tagataattt agtaaaaagt gctatggatg gtatttttaa catctcagct
    32281 atacgagata gccatatcac aggcttaatt gcttttaaat ataatgtggc gaacgaagga
    32341 aaaacagttg tgaggatcaa acgatattca gaatttgagt gggatacttc tgaaggattg
    32401 aacggagata tatgggaagc tacatttgat tttaaaccag tagcaacgcc aagaccaaag
    32461 tctaaattta gaggaaatgg gattatcact tactatccaa aagagtacaa cgactatttg
    32521 gaaaatatca agaatacgct taaagagaaa gatttggtaa atgatcaact gaaaaaggta
    32581 atgaatgctc ctatgggggt tatagctcag atagattatt tttaccaagt gagaaaagat
    32641 aagaaaaagc ttgattcact gatgcgtact tcacagccgg atattgataa tttagtcaaa
    32701 ggaacattgg acggaatttt taacaaggaa ataggcatca aagatagcag ggttgtaggg
    32761 ttaattgctt ttaaatacaa tacttttgaa aagtcgaaaa caaacgtgag actacaggag
    32821 atgggctgat gaataataat gaaaaaattc ttcatgtact tgatagcttt gaaataattc
    32881 aagaagaact aaaaaaatat cgggatgttt tggaacaaag atatgatttt gtcaatcagc
    32941 aaaagagtaa tcacatggat ttcattctca atatgaatga cttgaagaag aaattagttg
    33001 aacgaaaaga acaagaaaag ttgatcaaag cttactttga actcggtgaa aaagaagtta
    33061 aaaatgcaat ggaactgaat gaagagcgca gggttttaga tcagctgtta gaacaattat
    33121 tggtgatgtt tcaaaaagga cgaatagatg aggatttaat tgaagaagga ttgagaaaat
    33181 atccttcaaa ctcaggtatc ggaatagtgc tgaaagcgat tgatgaagaa gaaattgaag
    33241 ctttcatacc gccagaggat tttgaatcgg caatggaata tataaaatat tatagccaag
    33301 ggatcactgc ttttagagag tttgatcctg aagatgttat tcatgacttg aacaacctaa
    33361 aagaatggtg tgaaagttat ggagtcgatg attccggatt ggactacttg atcagtatta
    33421 tggaaatcga agaagagatg ccggataagc ctgatccaac agatatttta gaattgatcc
    33481 atgaagctag aaatccaatt gcctatattt ctagaggata tactgtattg gaatactata
    33541 agccttatat atcagcgatg aaccatttaa gacgtgtatt aagggaaaaa cgtgaatacc
    33601 gctcagtgtt gaatgcaacg aatagattag aaaaagctgt ttcagagcta gatgcttatt
    33661 atcgtgaaca ttacctgcaa gcaggtggta tgccaagaaa tacgaaagca aatatcagta
    33721 ggtatataaa aaaagctgag taggcggagt tagcccataa aatggcaaaa ggtaacctga
    33781 aaggggcatt ttgaatgaaa agggatgttt ttgaattgta cgtattagaa cgtctagaag
    33841 acggcatgat ggcacaagag ataattgatg aaattgatga tatgatcagc agatttggtc
    33901 aattggagtt gaatgatgga aacaaaaatt cggtagtgct aaaacaattt tggttgaaca
    33961 atttttcgta taaggatttt atcaaagaaa aggcaaatac attgtactga ctaaattcac
    34021 gaggagcagt gatgagagct tattgctaag agcagttagg ctcttttttg tttgtgtaaa
    34081 attatttgtt ttataataca catttataca caaaaaaata ttgacagata gtacacaaat
    34141 gtggtaggat atatttgtaa gcaagttaga ttaacaagtt aaaggaagag cagttatgaa
    34201 aaaagaagaa ctaatagata tatttcaaat tgttgaacga gcaaataata tgggaattat
    34261 gttttttgat cgtatttctt taaagatg
//
LOCUS       DACDCJ010000024        33972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDCJ010000024 DACDCJ010000000
VERSION     DACDCJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.024"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..808
                     /locus_tag="IV038_001134"
     CDS             68..808
                     /locus_tag="IV038_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8341366.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IV038_001135"
     CDS             complement(1232..1402)
                     /locus_tag="IV038_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8341367.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IV038_001136"
     CDS             complement(1596..1775)
                     /locus_tag="IV038_001136"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341368.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="IV038_001137"
     CDS             2162..3274
                     /locus_tag="IV038_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8341369.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IV038_001138"
     CDS             complement(3352..3645)
                     /locus_tag="IV038_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP8341370.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IV038_001139"
     CDS             3813..4127
                     /locus_tag="IV038_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP8341371.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IV038_001140"
     CDS             4148..6127
                     /locus_tag="IV038_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8341372.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IV038_001141"
     CDS             6140..6466
                     /locus_tag="IV038_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP8341373.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IV038_001142"
     CDS             6482..7000
                     /locus_tag="IV038_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341374.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IV038_001143"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IV038_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8341375.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IV038_001144"
     CDS             complement(8436..9941)
                     /locus_tag="IV038_001144"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP8341376.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IV038_001145"
     CDS             complement(10033..10623)
                     /locus_tag="IV038_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8341377.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="IV038_001146"
     CDS             10802..12448
                     /locus_tag="IV038_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8341378.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="IV038_001147"
     CDS             12497..13216
                     /locus_tag="IV038_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP8341379.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13815..14762
                     /locus_tag="IV038_001148"
     CDS             13815..14762
                     /locus_tag="IV038_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8341380.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14774..15694
                     /locus_tag="IV038_001149"
     CDS             14774..15694
                     /locus_tag="IV038_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8341381.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15735..17192
                     /locus_tag="IV038_001150"
     CDS             15735..17192
                     /locus_tag="IV038_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8341382.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17537..18151
                     /locus_tag="IV038_001151"
     CDS             17537..18151
                     /locus_tag="IV038_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8341383.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18144..19868
                     /locus_tag="IV038_001152"
     CDS             18144..19868
                     /locus_tag="IV038_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8341384.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19865..21316
                     /locus_tag="IV038_001153"
     CDS             19865..21316
                     /locus_tag="IV038_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8341385.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21789..23942)
                     /locus_tag="IV038_001154"
     CDS             complement(21789..23942)
                     /locus_tag="IV038_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8341386.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23944..24990)
                     /locus_tag="IV038_001155"
     CDS             complement(23944..24990)
                     /locus_tag="IV038_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8341387.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25140..26426
                     /locus_tag="IV038_001156"
     CDS             25140..26426
                     /locus_tag="IV038_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP8341388.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            26442..29144
                     /locus_tag="IV038_001157"
     CDS             26442..29144
                     /locus_tag="IV038_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP8341389.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29129..29986
                     /locus_tag="IV038_001158"
     CDS             29129..29986
                     /locus_tag="IV038_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8341390.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30057..30914)
                     /locus_tag="IV038_001159"
     CDS             complement(30057..30914)
                     /locus_tag="IV038_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8341391.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31125..33296
                     /locus_tag="IV038_001160"
     CDS             31125..33296
                     /locus_tag="IV038_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP8341392.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33364..33705
                     /locus_tag="IV038_001161"
     CDS             33364..33705
                     /locus_tag="IV038_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP8341393.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT        11052 a         5938 c         6969 g        10013 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtattttca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctgat aaacggtgtt caatcagctg
    13621 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13681 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13741 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13801 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13861 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13921 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13981 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    14041 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    14101 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14161 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14221 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14281 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14341 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14401 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14461 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14521 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14581 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14641 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14701 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14761 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14821 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14881 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14941 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    15001 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    15061 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15121 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15181 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15241 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15301 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15361 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15421 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15481 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15541 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15601 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15661 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15721 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15781 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15841 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15901 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15961 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    16021 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    16081 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16141 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16201 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16261 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16321 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16381 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16441 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16501 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16561 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16621 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16681 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16741 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16801 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16861 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16921 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16981 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    17041 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    17101 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17161 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17221 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17281 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17341 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17401 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17461 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17521 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17581 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17641 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17701 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17761 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17821 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17881 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17941 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    18001 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    18061 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18121 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18181 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18241 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18301 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18361 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18421 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18481 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18541 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18601 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18661 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18721 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18781 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18841 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18901 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18961 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    19021 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    19081 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19141 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19201 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19261 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19321 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19381 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19441 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19501 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19561 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19621 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19681 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19741 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19801 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19861 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19921 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19981 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    20041 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    20101 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20161 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20221 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20281 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20341 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20401 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20461 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20521 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20581 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20641 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20701 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20761 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20821 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20881 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20941 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    21001 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    21061 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21121 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21181 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21241 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21301 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21361 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21421 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21481 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21541 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21601 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21661 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21721 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21781 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21841 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21901 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    21961 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    22021 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    22081 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22141 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22201 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22261 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22321 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22381 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22441 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22501 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22561 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22621 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22681 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22741 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22801 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22861 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22921 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22981 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    23041 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    23101 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23161 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23221 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23281 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23341 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23401 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23461 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23521 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23581 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23641 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23701 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23761 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23821 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23881 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23941 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    24001 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    24061 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24121 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24181 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24241 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24301 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24361 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24421 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24481 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24541 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24601 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24661 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24721 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24781 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24841 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24901 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24961 aaatattgtc tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc
    25021 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    25081 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25141 tggcttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25201 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25261 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25321 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25381 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25441 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25501 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25561 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25621 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25681 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25741 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25801 atacttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25861 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25921 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25981 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    26041 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    26101 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26161 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26221 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26281 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26341 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26401 attactttga tattagagta gaatagtagg aggtatcgta aatgaaaaag aaagtatata
    26461 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26521 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26581 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26641 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26701 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26761 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26821 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26881 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26941 cccaatactc agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat
    27001 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    27061 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27121 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27181 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27241 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27301 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27361 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27421 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27481 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27541 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27601 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27661 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27721 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27781 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27841 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27901 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27961 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    28021 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    28081 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28141 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28201 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28261 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28321 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28381 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28441 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28501 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28561 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28621 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28681 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28741 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28801 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28861 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28921 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28981 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    29041 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    29101 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29161 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    29221 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    29281 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    29341 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    29401 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    29461 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    29521 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    29581 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    29641 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    29701 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    29761 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    29821 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    29881 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    29941 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    30001 caaaaagaac agagtcttat cattatctgc tgataagact ctgttctttt ttatttctat
    30061 tcccataatc caggtaaagc tttaccgact attttttctt gagtaaatgg atggatcatg
    30121 tgcagttcta atgcatggag catcagtcgt tcagctgttc tattttggta gagagggtct
    30181 ccaacgatcg gatgtccctt atgagctaaa tgaactctga tctggtgtgt acgtccggtt
    30241 tccaaaacac aatagacttg tgtttgttca gaaagtactt tgttgacagt gacatgtgtc
    30301 aaggcgctct gaccattttt atcatctacg accctttttc tccgatcatg gcgatctcgt
    30361 ccaattttat tggtgatggt catatctttg gcaatttctc cccatacgat ggcttgatat
    30421 ctccgataga tcattttttg ttccaacatt cttccaagta tcggcaagac aaatggattt
    30481 tttgcaaata aaacggcacc gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt
    30541 tgctgtttag tgtcgagata agcagctaaa tgattgagta aggtactgtt ttctgtcggt
    30601 tcatttggat gagtcttcat tcctgcaggc ttatccacta taatcaaatg ctcatcttca
    30661 tataaaacac ggatgtgctt cgcatctcct aattgaactt cttgataatt ataatctgtt
    30721 tcttcgaata acaatgtgat ctggtcattg ccctttactt catgatggaa caaagcaggt
    30781 tgttgattga tccaaacgtt tttacgtata cgcaaaaagt gtcgaacctt tctaggaact
    30841 aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg
    30901 atcgtgattt tcacaagtgt actcctttca gtgcaatcgc agactaattg taccattttt
    30961 aaaacatatt taagaaaaaa cttacatttt tctgttcatt cttatgttac tctacttgca
    31021 ctaacgtaaa aaaacatatt gaactacaac ttaagcagga agaaaacact ctttcactat
    31081 ggtaaaataa gacgattgaa tcaaatagac aggagaagaa gcaaatggat tttcaaagca
    31141 ttttagaaaa gataaaagct gctttgatca gtttttggcg ttggatcaga ccatatctcg
    31201 gacaattcca tcgatggcga aagcgcatct ggaaaaaata tcatgtgaac aaattgattt
    31261 tgctgattgg tttgatctgt gtcttagtga caagtatcta cctctttatt ttagcaaaac
    31321 aagcaaatgt cgaaacgtta aaatcaggat taagccaatc gacagtcatt tatgataaga
    31381 aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga
    31441 tttctcccta tatccaagat gcagtaatct cgacagaaga taggaatttt tacgagcatc
    31501 acgggtttga tatcaaaggg attgcccgtg cagcagtccg aatggtcttg tctggcggaa
    31561 catcaggggg cggaggaagt acgatcactc agcagcttgc caaaaatgcg tatctgacat
    31621 tagaccaaac gtttgatcga aaagcgaagg aattattttt ggcaatcgag atcgaaaaga
    31681 agtacagcaa agaagaaatc ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg
    31741 tatggggtgt ccaagacgct tccagaaagt attttggagt agatgcaacg aatgttacgc
    31801 tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg
    31861 atcacccaaa aaatgcgaat gatcggaaaa acacagtact aagtgtaatg gaagaaaacg
    31921 gaaaaatcac aaaagaacaa gaacaagcag aaagccagac agatatcagc gcgtatctta
    31981 atgacacata cgataattca gattctggtt atcgttatcc ttattatttt gatgcagtga
    32041 tcgatgaagc agttaacgag tatggtcttg atgaagaaga tgtcatgaat aaaggctata
    32101 aaatctatac agctttagat caggattatc aaaagcaatt agaagctact tatcaaaaca
    32161 gtgcattatt cccagcaaat gcttcagatg gtgccatggt ccaatctgct tcagtagctt
    32221 tagatccgaa tacaggtggt gtacaagcac tagtaggacg aagaggagat catgtcttta
    32281 gaggattcag ctttgctaca cagatgcgtc gttcaccagg atcaacgatc aaaccattaa
    32341 gcgtatatac accagcactg gaagctggat acaagccttc cagtatcttg aaagacgaac
    32401 cgcaaagtta ttacgatgcg cataattttg acgggacata ccaaggagaa gtaccgatgt
    32461 atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg gcttttgcat gagatcggtt
    32521 tacaaaaagg atatgataaa gcagaagaat tcggcctgcc tttagcaaaa tctgataagt
    32581 attatggctt agcccttggt ggactggaaa aaggagtatc tccattgatc atggcgggtg
    32641 cttacagtgc atttgccaat gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg
    32701 ttgattcgac tggagcggtg atcgtcgata acacaaacaa aaaaccaaaa caggtcatat
    32761 caaaggaaac agccgatgaa atgaccagca tgcttcttgg tacattttca aatggaacag
    32821 cggctgctgc gaatcctgca ggttatacga tagcagggaa aacaggaacc acagaaacta
    32881 atttcgatgc cacaaaagca aatgaccaat ggatgatcgg ttacacgcca gacgtagtga
    32941 tttcgacatg gatgggttac gaagaatcca gcaagctgca ctatttggaa gggacaagtg
    33001 gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg catccttccg tatacttcaa
    33061 aaacaggatt tagtgtagca gatgcctatg ccacaggagg acaagtggta gctgcagatc
    33121 aagttggaaa cacaggaaat tctaatggag agtctggaaa ttggcaagat aacttgaatc
    33181 aatatggtga acaagcaaaa gaagggctga aaaacttcgg cgatatggtg aaagaaggcg
    33241 ttcagggaat tggtgaagcg gctaaagacc tctggcggaa atatcaagga gagtagcccc
    33301 tttgaaaacg aataacccca tgttacaata gagagtaact actaaaagaa aagagggcat
    33361 aacatgtcaa acatctacga tagcgcgaat caaatcgaac gtgagatccg tgaattacca
    33421 gaattcaaag cgttgaaaga agcgtacgag aaagtacaag caaacgaaga agcacacaaa
    33481 ctgttcaaag acttccaagc gatgcaaatc gaattacaag aaaaacaaat gagtggacaa
    33541 gagttcagcg acgaagatgc aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa
    33601 gaagtaatca atgacttgat gcaaaaagag caaggcttca gtacaatcat caatgacttg
    33661 aaccgtatca tcatgacacc agttcgcgac ctttacagcg aataaaacta ttttatgaaa
    33721 caacagaggg tgtgaaagag acgttcggcc ttgagcgatg acaaaattgc ttcttattgc
    33781 cgataggctg tctcttgaac accatttacc aagaggtcgt gagcctaccg tttaactctg
    33841 agttacaagg aacaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    33901 accgaggagt tggcaggaga acaccgttta ttcagaaata ggagctacga caaaagtctt
    33961 gtcatagctc ct
//
LOCUS       DACDCJ010000025        32533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDCJ010000025 DACDCJ010000000
VERSION     DACDCJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.025"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IV038_001162"
     CDS             131..505
                     /locus_tag="IV038_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8341394.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IV038_001163"
     CDS             complement(619..1248)
                     /locus_tag="IV038_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8341395.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IV038_001164"
     tRNA            complement(1382..1454)
                     /locus_tag="IV038_001164"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IV038_001165"
     tRNA            1668..1741
                     /locus_tag="IV038_001165"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IV038_001166"
     CDS             1696..1845
                     /locus_tag="IV038_001166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341396.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IV038_001167"
     CDS             2338..3666
                     /locus_tag="IV038_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8341397.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IV038_001168"
     CDS             3868..4701
                     /locus_tag="IV038_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP8341398.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IV038_001169"
     CDS             4717..5454
                     /locus_tag="IV038_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP8341399.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IV038_001170"
     CDS             complement(5609..6577)
                     /locus_tag="IV038_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP8341400.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IV038_001171"
     CDS             6809..7642
                     /locus_tag="IV038_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP8341401.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IV038_001172"
     CDS             7676..8515
                     /locus_tag="IV038_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8341402.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IV038_001173"
     CDS             8541..9101
                     /locus_tag="IV038_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8341403.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IV038_001174"
     CDS             9300..11021
                     /locus_tag="IV038_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP8341404.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IV038_001175"
     CDS             11186..12328
                     /locus_tag="IV038_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP8341405.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IV038_001176"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IV038_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP8341406.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IV038_001177"
     CDS             14128..14775
                     /locus_tag="IV038_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP8341407.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IV038_001178"
     CDS             15168..17813
                     /locus_tag="IV038_001178"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP8341408.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IV038_001179"
     CDS             18123..19436
                     /locus_tag="IV038_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP8341409.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IV038_001180"
     CDS             19426..20079
                     /locus_tag="IV038_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8341410.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /locus_tag="IV038_001181"
     CDS             20134..20826
                     /locus_tag="IV038_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8341411.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="IV038_001182"
     CDS             20976..21176
                     /locus_tag="IV038_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8341412.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="IV038_001183"
     CDS             complement(21430..23109)
                     /locus_tag="IV038_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8341413.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="IV038_001184"
     CDS             complement(23111..23323)
                     /locus_tag="IV038_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP8341414.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="IV038_001185"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="IV038_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP8341415.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="IV038_001186"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="IV038_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP8341416.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="IV038_001187"
     CDS             27298..27495
                     /locus_tag="IV038_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341417.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="IV038_001188"
     CDS             27648..28133
                     /locus_tag="IV038_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP8341418.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IV038_001189"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IV038_001189"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP8341419.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="IV038_001190"
     CDS             29010..29537
                     /locus_tag="IV038_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP8341420.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="IV038_001191"
     CDS             29628..30365
                     /locus_tag="IV038_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8341421.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="IV038_001192"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="IV038_001192"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP8341422.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="IV038_001193"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="IV038_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP8341423.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6874 g         9365 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DACDCJ010000026        31066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDCJ010000026 DACDCJ010000000
VERSION     DACDCJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.026"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(61..2424)
                     /locus_tag="IV038_001194"
     CDS             complement(61..2424)
                     /locus_tag="IV038_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP8341424.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IV038_001195"
     CDS             2872..3093
                     /locus_tag="IV038_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8341425.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IV038_001196"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IV038_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP8341426.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IV038_001197"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IV038_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP8341427.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="IV038_001198"
     CDS             complement(5894..6205)
                     /locus_tag="IV038_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP8341428.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="IV038_001199"
     CDS             complement(6202..6495)
                     /locus_tag="IV038_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP8341429.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IV038_001200"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IV038_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP8341430.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IV038_001201"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IV038_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP8341431.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="IV038_001202"
     CDS             complement(8333..12685)
                     /locus_tag="IV038_001202"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP8341432.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="IV038_001203"
     CDS             complement(12897..14606)
                     /locus_tag="IV038_001203"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP8341433.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IV038_001204"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IV038_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP8341434.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="IV038_001205"
     CDS             complement(16103..16903)
                     /locus_tag="IV038_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP8341435.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSTVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="IV038_001206"
     CDS             complement(16900..17712)
                     /locus_tag="IV038_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP8341436.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IV038_001207"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IV038_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP8341437.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="IV038_001208"
     CDS             complement(18467..19189)
                     /locus_tag="IV038_001208"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP8341438.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="IV038_001209"
     CDS             complement(19325..20206)
                     /locus_tag="IV038_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP8341439.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IV038_001210"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IV038_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP8341440.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="IV038_001211"
     CDS             21446..21925
                     /locus_tag="IV038_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP8341441.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="IV038_001212"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="IV038_001212"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP8341442.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="IV038_001213"
     CDS             23226..24011
                     /locus_tag="IV038_001213"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP8341443.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="IV038_001214"
     CDS             complement(24357..26048)
                     /locus_tag="IV038_001214"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP8341444.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IV038_001215"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IV038_001215"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP8341445.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IV038_001216"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IV038_001216"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP8341446.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IV038_001217"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IV038_001217"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP8341447.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="IV038_001218"
     CDS             30332..31033
                     /locus_tag="IV038_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8341448.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8387 a         6626 c         5507 g        10546 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagt actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcgt tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagatggcat tgccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattccg tcaaacatac gtcctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agactgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacgacctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg ttcggaattt tgattgccaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcata
    29461 tagactgcta gaacgtactt cgatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 cttcgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc gtttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaattt ttttgaataa attaaaaaaa taaattatat tatcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cgggctgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg ataaat
//
LOCUS       DACDCJ010000027        29919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDCJ010000027 DACDCJ010000000
VERSION     DACDCJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.027"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..1117)
                     /locus_tag="IV038_001219"
     CDS             complement(53..1117)
                     /locus_tag="IV038_001219"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP8341449.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1114..2199)
                     /locus_tag="IV038_001220"
     CDS             complement(1114..2199)
                     /locus_tag="IV038_001220"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP8341450.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2212..3189)
                     /gene="mvaD"
                     /locus_tag="IV038_001221"
     CDS             complement(2212..3189)
                     /gene="mvaD"
                     /locus_tag="IV038_001221"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP8341451.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3182..4126)
                     /gene="mvk"
                     /locus_tag="IV038_001222"
     CDS             complement(3182..4126)
                     /gene="mvk"
                     /locus_tag="IV038_001222"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP8341452.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4448..5101
                     /locus_tag="IV038_001223"
     CDS             4448..5101
                     /locus_tag="IV038_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8341453.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            5186..6091
                     /locus_tag="IV038_001224"
     CDS             5186..6091
                     /locus_tag="IV038_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8341454.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(6093..6725)
                     /locus_tag="IV038_001225"
     CDS             complement(6093..6725)
                     /locus_tag="IV038_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP8341455.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7045..7569
                     /gene="infC"
                     /locus_tag="IV038_001226"
     CDS             7045..7569
                     /gene="infC"
                     /locus_tag="IV038_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP8341456.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7641..7841
                     /gene="rpmI"
                     /locus_tag="IV038_001227"
     CDS             7641..7841
                     /gene="rpmI"
                     /locus_tag="IV038_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP8341457.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7894..8253
                     /gene="rplT"
                     /locus_tag="IV038_001228"
     CDS             7894..8253
                     /gene="rplT"
                     /locus_tag="IV038_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP8341458.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8505..9842
                     /locus_tag="IV038_001229"
     CDS             8505..9842
                     /locus_tag="IV038_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP8341459.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            9890..12019
                     /locus_tag="IV038_001230"
     CDS             9890..12019
                     /locus_tag="IV038_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP8341460.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11994..12683
                     /locus_tag="IV038_001231"
     CDS             11994..12683
                     /locus_tag="IV038_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341461.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13091..13777)
                     /locus_tag="IV038_001232"
     CDS             complement(13091..13777)
                     /locus_tag="IV038_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8341462.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13913..14107)
                     /locus_tag="IV038_001233"
     CDS             complement(13913..14107)
                     /locus_tag="IV038_001233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341463.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14157..14597)
                     /locus_tag="IV038_001234"
     CDS             complement(14157..14597)
                     /locus_tag="IV038_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP8341464.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(14601..15446)
                     /locus_tag="IV038_001235"
     CDS             complement(14601..15446)
                     /locus_tag="IV038_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP8341465.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15595..17013
                     /gene="pepV"
                     /locus_tag="IV038_001236"
     CDS             15595..17013
                     /gene="pepV"
                     /locus_tag="IV038_001236"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP8341466.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17070..18017)
                     /locus_tag="IV038_001237"
     CDS             complement(17070..18017)
                     /locus_tag="IV038_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8341467.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(18240..18590)
                     /locus_tag="IV038_001238"
     CDS             complement(18240..18590)
                     /locus_tag="IV038_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8341468.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            18790..19869
                     /gene="pepA"
                     /locus_tag="IV038_001239"
     CDS             18790..19869
                     /gene="pepA"
                     /locus_tag="IV038_001239"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP8341469.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20013..20333
                     /locus_tag="IV038_001240"
     CDS             20013..20333
                     /locus_tag="IV038_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP8341470.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            20355..20819
                     /locus_tag="IV038_001241"
     CDS             20355..20819
                     /locus_tag="IV038_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8341471.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            21024..21629
                     /locus_tag="IV038_001242"
     CDS             21024..21629
                     /locus_tag="IV038_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP8341472.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(21668..22957)
                     /locus_tag="IV038_001243"
     CDS             complement(21668..22957)
                     /locus_tag="IV038_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP8341473.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            23385..23864
                     /gene="rlmH"
                     /locus_tag="IV038_001244"
     CDS             23385..23864
                     /gene="rlmH"
                     /locus_tag="IV038_001244"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP8341474.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            23949..24458
                     /locus_tag="IV038_001245"
     CDS             23949..24458
                     /locus_tag="IV038_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP8341475.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            24558..25208
                     /locus_tag="IV038_001246"
     CDS             24558..25208
                     /locus_tag="IV038_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341476.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            25659..26597
                     /locus_tag="IV038_001247"
     CDS             25659..26597
                     /locus_tag="IV038_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8341477.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            26610..27662
                     /locus_tag="IV038_001248"
     CDS             26610..27662
                     /locus_tag="IV038_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8341478.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            27928..28563
                     /locus_tag="IV038_001249"
     CDS             27928..28563
                     /locus_tag="IV038_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8341479.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            28754..29545
                     /gene="msrA"
                     /locus_tag="IV038_001250"
     CDS             28754..29545
                     /gene="msrA"
                     /locus_tag="IV038_001250"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP8341480.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         9315 a         5432 c         6174 g         8998 t
ORIGIN      
        1 gcttttactt ccgccgtttg tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
       61 gtcccacgct cttttctgtg cgtcttccat acttcactaa gtcgatccct cttgcttcgc
      121 accaatcctt cactggtccg gaaaagatca gagattgttg gtgtagggcc gcggtattcg
      181 ttgcgccgac catagtataa agcagacgaa gctcttcttg ccattgcttc atcagtataa
      241 tagtttcttc tacgccgtga ttcatcagat gcgtcagtac ggttcctgaa gtacccacgg
      301 catttgcgcc gagacaaagg gctttaaata tgtcataggc attgcggata cctccggagg
      361 ctaggatttc catagaagtg tctgcttcat ttgcttcaag caatgaagag actgtcgatt
      421 gcccccagtc agctaaataa ctcagttccc gcttggagcg acgggcgttt tcgatctggg
      481 tgaagcttgt tccgcttcga ccgctgatat caactgtatg aactcctaaa gcagctagat
      541 cattgagcgt ttctcttgtc atgccaaaac cgacttcttt tacgatgagg gggacgtcaa
      601 tggctgtttg agtttcttga ataagtactt tccaattggt aaaatcacgg tctccctcag
      661 gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa ggcatctgcg tgaaacaatc
      721 ggatagcttc tttggctctt tcgacagaag tccctgcacc gatgttggca atgattttcc
      781 cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc taaagaaggg actttcaagg
      841 cagcgctgac tgaaccagtg gcaatcatca aatctgcttc tcttgcgacg attgcaagat
      901 cttggttgat tttttttgtt ttttcgcttc cgccagtcat cgcattgata taaaatgggc
      961 tgcttagtgt aaacccaact gcctgtgtac ttagatcgac atcagaaact gctatttgcg
     1021 gcaaagggtt atggatgatc cgtacgaagt caaattcgtt tttttgttta tcatgaaagg
     1081 ctttagctag tgaaacatgt tcgtcttttc gattcatcgg ttttcctttc tttgatcgct
     1141 atagacatgt aacggcagtg gagtgatttc tgctttttcc catgcactca ttaaaggaag
     1201 aatgccagat ttctggtcaa caattacgat tccgcaatcg cccccacctg caccagaaga
     1261 ttttgcggct ccttcatact gttcagctaa attacacaat ttgttcaaag caggcgtttc
     1321 gatgacgacc ccagtgattg cagataaatc atgcagtaat tgtcggtttt ttcgaatcat
     1381 ctgttggatc aacgttacat tattttcttt aaaccctttg atcatttcgt tgacacattc
     1441 tgtactattt tttaagaaaa gctgataagc caccatttta tcttctctcg aacggtgaac
     1501 ttgatcgacc aaatcagaag tagaggcagg gctacccgtc caaccaatca ataaacgtaa
     1561 atcttcagga gcaatcaatg gctcaatgga tagacctggc caatccaggg caagtaactc
     1621 actgatagaa tgctgagttt cttgttcttg gagccaagga tggtcgaagg ttgagaaagc
     1681 gatccagcca ccataacagc tagcagcgat gtcgccgcag gaaccattat cttgaactgc
     1741 taaattggct agtgctgcaa tcttgaaaat ctccaactga gataaattca acgcgtaaaa
     1801 aacattcaag gctttgaccg ttgcaacggt cacggcaccg ctagaaccca atccatattt
     1861 ccggccattt gaactgtcta actcacttgt tactttcaga tcataaaagg ataaaagaat
     1921 gtttttttct tgtgcatact tttcagtcaa gcgaatagca gcaaggatat aatgaaaagg
     1981 attttcccga atatctaaaa ctaattctcc gttgcgtctt gtccaacgta caggcatccc
     2041 actatattgt gcagattgga tacttccgac ttttcgtgca gattctacag tgactgtcac
     2101 gaattgatcg actgcagcga taactgctgg atggcctgtt tcaacaactg catattctcc
     2161 ggcaatatag agtttgcctg gtgcagatac ttcaatcata aaaatctccc tttattcaat
     2221 aatcgcaatt cctggaccag catgtgcagt aattacttgt tctttgctaa ataatttggt
     2281 aaatgtttct tgtacctcag ataaatggct gttttccact aaaactttga cattagggcc
     2341 agcatccatc gtaaagtagc atggaatgcc ttgctttctc aattgtcgca ccgcatccat
     2401 cgcttttaag ctgtcaggag accagtaagt gaatggaggc tgggcagcta aagtcgttcc
     2461 atgcattttt agtccgtttc tttccatcgt ttctccgaga agttggaaat cttttgtttt
     2521 gattgcttgt ttcaattgat atagatcccc ttcaacggaa tctaaccagc cttgataaaa
     2581 actggatgtt tcgactgtcc gacgcatccc atttctgctg gacacttctt ttttctggtc
     2641 gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa gaatcggaag ggactggctt
     2701 agcgtaagaa cttaagtcgt catggccttt ttcccattcg acgaaaccac cgaaaatact
     2761 tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa agagagagat catctaatcc
     2821 aagcttaaga gcagtattgc aagcgcctgc taaagcagct agcccgctgg cagaggaggc
     2881 taatccagct gcggtaggaa cgaaattctg actgattact gaagctttta gtgagaggcc
     2941 ggcttcttta cgaaaaaggt caagaaactg actgactttt tttgttgctt tttcgtcttg
     3001 caaggtgcca tctagataaa attcatctac catatagcta tcagaaaaga tgacttctgt
     3061 ctctgtataa aatgcatcca atgttaacga aaggctgttg ttcattggaa ggatgagttc
     3121 ttcatttttc ttaccccaat atttgattag agcaatgttg gtatatgcgc gtgctttgcc
     3181 tttaaacata ggtatgtact cctaatcctt gtatccaagt ttcagcagca cccgcatctt
     3241 caagtgcttg actgatttct tgggcttttg tttttgtttg tgctaatgca atcatacagc
     3301 caccgcgtcc accgccagtt aatttggcac ccaatgcccc ggtatctttt gctgtttgga
     3361 tcaatagatt aagaaaatca ttgctgattg tcagcttttc cagaagtgat tgtgattcat
     3421 ccattgtctg tgccaacgtt tctggtgaat tttcaatgat tgcttgtttt gcttgcttcg
     3481 tcaagtatcc taatttttgg atcatttgtc cagtttcatg aggctgggtt tcaaagaggt
     3541 gagcgacatc tttgacggct tctcttgttt gtcctttgat tcctgtatca gcgacaatca
     3601 aaaaagcatc gatgttcaaa gaaaagtagt cgaaaggatg cccttttgta aaataaatcg
     3661 gctgcaagct gctagtagcg gctgcatcga ttccactagg attaccgtgg gcgatttttt
     3721 ccgaaagctg gacattttct aatagtattt cacgagacaa aggaaatgct aagtaatcat
     3781 aaaaagcacg agtgactgct gtggcgactg cagcgcttga tcccattcca cgttcagcag
     3841 gaatcgtgct ttcaatggtc agttgcaagt ttttttggat gtcatgctgt ttttttaagt
     3901 gaatgaataa ttttttgata ttttttaatg catgaggagc ttcggctaaa tttcctgaat
     3961 agtaggaaga aaccagttga tcgtccattg catccagctc ttcaaggaat gcggtgactt
     4021 ttgttgcata gaaaggaaag gcaatcgctg gttctccata aacaaccgcg tgctcgccca
     4081 tcaatatgat ctttccgctt gattctcctt ggccatagtt tgccataatc taactccttc
     4141 tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca aaacgagttg cgctgcttca
     4201 cgaatttttt tgtccactaa gggtctcaaa aaacattcct cctttatttt actaaaacca
     4261 tctcagaagt aaaactaaga atacgttttc taaagaaaaa acactttttt cttaagaatt
     4321 ttttgacttt ttttcttctt tgactagaaa aaaatacaat tgacactact agatattggg
     4381 ggtatattta aaaaggttgg agttaaacac aatatttagt gatgaagtga gctggaggta
     4441 aatgagcgtg atcgttttag caggtacaat tggtgccgga aaatcaagtt taacagaaat
     4501 gatggctgaa cactttgata gtcaggcatt ttatgaatcg attgatgata atgaagtctt
     4561 gcccttattt tatgcaaacc ctgaacagta tgcttttttg ctgcaaattt attttttgaa
     4621 taaacgtttt gcaagcatca aacaagcaat gaaagatgac aataatgtat tggaccgttc
     4681 gatttatgaa gattcactgc ttttccatct aaatgcagat ctaggaagag caacagaaac
     4741 agaagttcgt gtgtatgatg aactgctgga aaacatgatg gaagaacttc cctatgctgc
     4801 ccataaaaaa catccggatt tattagttca tattcgtgta tcttttgata cgatgttgga
     4861 gcgtatcgaa aaacgtggtc gttcgtatga acaattgtcc tttgatccaa gtctatacga
     4921 ttattacaaa gaattgaaca gacgttacga tcaatggtat gaagaataca aagaaagtcc
     4981 aaaaatccaa atcgatggag accgctataa ttttgtggaa gatcctcaag caaaagaaga
     5041 agtcttgaaa atgatcgaag aaaaattagc agagattcgc caaaccaaag tagccatttg
     5101 atttacagtt gctcaaataa agggtaaacc tttatttgag cgactttttt ctttatactg
     5161 gaaacaaatg tcaggaggat agagtatgcg ggaaaaacac tattttaatc aagaccgtaa
     5221 tttttggatc aatgtacagc aaaacgatgt gctagctgtt aaaaacttaa aagaagaata
     5281 tggcattagt gacgagatgc tgacttattc tctagataaa aatgaacggg cgcgcgtaga
     5341 gtacgattct tttgatgaag cgttactgct tgtatcaaat gttccgcacc agcaaaaagt
     5401 cgataatcat tatgaaacta gtcctatcgc ttttatttta aaagaagacg gtttgtttac
     5461 atttaccacg ccgaataccg agtacgtgat tcgactaatt cgatcgcttt tggaacgaat
     5521 gccagacatg agtgtataca gtttattgtt tcgtacattg tttttgattt ctgattcctt
     5581 ttttccttta atcgaggaag tgaatagtga acgtcagcgg cttaatctga aattaagaga
     5641 aaaaacaacg aacaagaatc tgctgcaact ttcagattta gagatcggtt tggtttactt
     5701 agttacagga acaaaacaaa atgtagtact tttagaacaa atcaaggcat tggctattta
     5761 tcgcaagttg tcagaaaagg aaaaagaaga gttggacgat gcgctgatcg aagcacgaca
     5821 agcagttgaa atgacgaact tagcttcgca gattttagac cagctctctg gcacttataa
     5881 taatttgctg aataacaact tgaatgacac gatgaaattt ttgacggtct ggtcgctgat
     5941 cttgaccgtt ccaactattg tgacaggatt cttcggaatg aatctccagt taccctttac
     6001 ccattccgtt ttcggatggg gaatcgcctt gatcatcagt ttggtcttat ctatttggat
     6061 gcttattgca ctgtggcggc ggattcgttg atctagtctt tgatctgcaa accaagggct
     6121 tgggcgatca caagtgcttg ctcttggttg acgattagtc ccgccaaaga atcttgagat
     6181 aaagcgattg ctgtgaagaa attagtcgta aagtctaatc ccttcagaga agttcgaaac
     6241 cagttcgaac cggtcaactg attgtcagac aaaaaaaggt ttttccaagt gacctcataa
     6301 aattcgctgt cttctaattg acaatgatcg aaggagacag ctttcaagtt tgtactacta
     6361 aaggaagcca tcgaagcaat acaatcagtg aaagtacagt ctcgaagata actttcggca
     6421 aaattcgtac ctgttaattt acattgatga aacacgactt ggtggaaact gccaccaatc
     6481 cactccagat tggaaaaatc acatttttca aaaatgacat tactgcattc aaaatgacgt
     6541 aaaactgttt tttgcatcgt aagtttttgg atatgactct ctcttaatac catatttttg
     6601 gcatcgagat agctttggtc acccccgttg atcagaagac cagagaagat ctcctcatct
     6661 tctaaataaa actctccctt tgtaagaaga ggaagagaag gtggaatagg ttcgattttt
     6721 ttcatgtggg ttcacctcgt ttttctttag tatagcaaag aagcgggaat cactgctaga
     6781 aagtctgcta atctaatcat tttcagaaaa ggaagacaaa ctgtatgaaa aacattgaca
     6841 aaaagattag tttttgctat actatgatgg ttgagaaata aaagcagaag cacccgcttc
     6901 tcgccttagt tgataatgtc actgggctag atagttttca ctggttctat taggtgaagt
     6961 acgcgcgggt tctgtaagag aactcgtttt tttattgccg tttattcggt aagcttttct
     7021 ttctctcata atatttggag gtgaatgacc atagcaaagg atatgatggt taacgacggc
     7081 attcgtgcac gagagttacg attgatcggt tcagatggtg agcaattagg agttaagaca
     7141 aaagcagaag cattgaatat tgcagaacaa gcaaacttgg atcttgtgtt agttgcccct
     7201 ggtgcgaaac caccagttgc gcgaatcatg gactacggta aataccgttt cgaacaacaa
     7261 aagaaagacc gcgaagcgcg taaaaaacaa aaagtgatca atgtaaaaga agttcgctta
     7321 agtcctacga tcgacttgaa tgacttcaat acaaaacttc gtaatgcacg taagttcttg
     7381 gaaaagggcg ataaagtgaa agcttctatc cgtttcaaag gccgtgccat tacccataaa
     7441 gaaattggtc aaaaagtctt gaatcgctta gctgaagaaa ctgcggatat tgcaacagtg
     7501 gaacaaaaag cgaaaatgga cggacgcagc atgttcttga cgctggcacc gaaaaacgac
     7561 agtaagtaat ttgaatgact aactggctgt tggacagccg atagtcggat aacgaaagat
     7621 tttgaggagg aaatatagtc atgccaaaac aaaaaacaca ccgcggatca gcaaaacgtt
     7681 tcaaacgtac tggtaaaggc ggattgaaac gcttccgcgc gtttacaagt caccgtttcc
     7741 acggcaaaac aaaaaaacaa cgccgtcaat tgcgtaaagc aagcatggtt tctagtggcg
     7801 acttcaaacg tattcgccaa caactagcaa aaatgaaata agaagtacaa ataacagaat
     7861 ctgaagattt atttttatta ggaggaatta tacatggcac gtgttaaagg tggaacagta
     7921 actcgtaaac gtcgtaaaag agtgctaaaa ttagcaaaag gttactacgg ttcaaaacac
     7981 actttattta aaacagcaaa agaacaagta atgaattcat acaactacgc atatcgcgat
     8041 cgtcgtcaaa agaaacgcga cttccgtaaa ttgtggattg ctcgtatcaa cgcagcagct
     8101 cgtatgaatg gcttgagcta ctcaaaatta atgcacggtt tgaaagttgc tgaaatcgac
     8161 atcaaccgta aaatgttagc tgacttggct gttaacgatg cagcagcatt tactgcattg
     8221 gctgaccaag caaaagaagc tttggctaaa taagctaaac aattataaag aggaaagaag
     8281 ataacgaaaa gttatgttct ttcctctttt tttatttaca atcctccatc aaatgaaaca
     8341 ggacaataac gtataagtct aaaatagcat aattaaggtt ttttaattga aaactgaaca
     8401 tttatggtca ccctttaatg aaagcgctaa ctacataagt tagaatgagt ttgactgata
     8461 tattagtttg cttatcagaa aaacaaaatg gacggaggaa taacatgagt gatattatta
     8521 ttggtatttt gttagttatc tcattctttt tcatggtctg gtactgtgta aaaggctaca
     8581 acttgatggt cggctttgcc atcatggcta ctgtatggat gggattagcg ctagtcggaa
     8641 acacgttttc accaaacccg gcgatggaag ggcaaggagt aattgatgtg ttgacgcata
     8701 tctatacgac ggggccagct gagtacgcca aatcgatctt ggtaaatgtc ttctttggtg
     8761 cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc taccctgatt cgaaaggtag
     8821 ttgaacttgg aggggacaag ccaagaatca cgatgagctt actatgtatt gtaaccgctg
     8881 ttatattcat gtcgatgaca ggaatcggac cagtgatttc tattgcagtt attgtgttgc
     8941 caatcttgat gtcattaggt atttcagtac cggttgcact gttctcattt atgggctcga
     9001 ttatggctgg aatctttgct aatatcgtca atttcaaaca atatcaaacg atttatgctg
     9061 gtttcaatcc agcggctgaa agctatactt ataatgacta tttccaaatt ggtatgattg
     9121 ggatgattgt aagcttagtt gtggtactaa cggtcgctaa tatatcaatg aacaaaaaga
     9181 aacgctatgc aatggcagca aacgtaccag ctgaaggtgg cgacgcacca atgatctcat
     9241 ggttagccgt attattacca gttttagggg tcgttctttt agatcttcca atcatcttag
     9301 gttttattct agcaggtatc tgggcattgc tgtttactgg aaaattgcgc ggaggttata
     9361 aagaaatctg tcgtcagttt gcgaaattat ttacagatgg cgcagtagat gttgcaccaa
     9421 tggttggttt cttgatgacc ttagcgatgt tcaataattc agctgcgtat gcttctcctt
     9481 atttttcagc tatttttgga gattggatac cgcaatcacc attagtcttg gcaatcgtat
     9541 ttgctatatt gacaccacta ggatttttcc gcggtccaat gaatctagtc ggttcaggct
     9601 cagctatttt agctgtcgta ttagctgtta atccaacgat gtcgccagcc tttttgttcc
     9661 cgttatttgc aatcacaacg attgctcctc agcatttaga tattacacaa tcttgggttg
     9721 cttggggatt aggttataca aaagtaactt ctcgagaata tatgaagaaa tccatcccaa
     9781 ctggttggat catcggagcg atcttatgcc tgatcacgtt cttattgtac gggaacgcct
     9841 agtaaacaaa acgtcgataa aaacgattag atgaatggag gaaaagaaaa tgaaacgttt
     9901 tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa gcagtcgcaa tcgataatgc
     9961 gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc cacgatgatg tccgtggagt
    10021 agccgcaggt gtcgttcagt cttttcaaaa ttgtttgcgg gaaaataata tcagtccaga
    10081 agatgttgtg tttgttgcac attctaccac acaagcgact aatgcactga ttgaaggaga
    10141 cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga ttagaaggtt ttttagcaaa
    10201 acgacaaact agattgaacg atatcgattt agggaataag aaaaaaatag agattgtcaa
    10261 tgcattctta cctgtcaaac acttaaatgt agaccgtgtt tcagaaacga tttcttcttt
    10321 agaaagagaa agagcggagg tcttggtttc ttctatggca tttggagtag ataatgggga
    10381 accagaacga gttgtttatg aagcagcatc ggtaaaatcg atccctacaa cgatggcttc
    10441 tgacatcaca aaattatatg gattgacaag aagaactcgg acagcagcaa tcaatgcatc
    10501 tattttacca aaaatgctag atacggcaac ctctacagaa gattccgtac gagaagcagg
    10561 ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga gtaatggaaa tcaatgaaat
    10621 gaaaaaacga cctgttttaa ccatgctttc cggtccagca gcatcagtca tgggctcttt
    10681 gatgtacttg agagcgtcta atggtgttta ttttgaagta ggcggaacaa caaccaatat
    10741 cggggtcatt aaaaatggac gtccagcaat cgattattca attgtaggtg gccacccaac
    10801 atatatctct tcattagatg ttcgggttct aggtgtggca ggcggttcga tggtacgggc
    10861 taatcagtca ggaatcatcg atgtcggtcc acgttctgca catattgccg gtctagatta
    10921 tgcagtattt acggagacag aaaaaatcaa aggtcctaag gttgaatttt tctctcctaa
    10981 agaaggagac cctgctgatt acgtcaaggt agtgatggaa gacggagaag aagtgactat
    11041 tacaaataca tgtgcagcca atgtccttgg attagtgcaa gaagaacatt tttcttatgg
    11101 aaatgtccca tctgcacgaa aagccatcca agcattagct gattattgtc atacgacggt
    11161 tgaagacatc gcagaacaga tcatggaaaa atcctatgct aagattgaac cggtgatttt
    11221 agaattggca gacaagtatc acttggagaa agatcagatt tctcttgtag gtgtaggggg
    11281 cggcgctgcc tcattgatta cttactttag taataaaatg ggtgtgaaat attcgattcc
    11341 tgaaaacgcc gaagtgattt cttctattgg tgtagcactt gcaatggtcc gagatgttgt
    11401 agaacgtatc atcccttcgc caagtaaaga agatatacga tcgttgaaaa atgaagcgat
    11461 gaataaagcg attgaatcag gcgctacacc agaatcgatc gaagtccacg tagaaatcga
    11521 tccacagact tcaaaagtaa cggctatcgc tactggttct acggaagtga aagcaaccga
    11581 tctaaccaag gagatcacaa cagaagaagc actagagctt gctgcagagg atatgcgatt
    11641 aaataaaaat gaagtttgct tattggaaaa tacacctttc ttttatgtat gcggcgaaca
    11701 gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat caaaaaggat ttatcaaggt
    11761 ccaacgtgga catgcttcat gtatgaaaac aactgcagcc aattatatga cagctgtcga
    11821 acaactttgg gaagatatgg cagtttatca aactgaattg attgcccgac cagaatttta
    11881 tctctgctta ggagcaagag tctctgattt tacagcgacg gatctggaac aactacagct
    11941 tttgatggat ctggaagtat ctactatgga accggaggaa gaagtgatcg tcgttgccgg
    12001 aaatatcaaa caaacataag ttagaagagt tactaaacga attacaacag atccccgaag
    12061 aaatttgggg attctaccag tttcggcgag atttattttg gaaaaagatt cctttgtcca
    12121 agcagaaaat tcttatacaa caatcaatcg attgtggtat cgaaacggca tgtagcatca
    12181 aaaaaaagta cccttttgcc gatgtaggag aaatttgtga acaaatggct gttccaatcg
    12241 tttcctgtga atcagagcaa ataaacgaac gaatcacttt tgcaacttat gcagaagatg
    12301 aagggattcg attgatgacg gagccgttag aaaaattaaa atgttcagga ctagcttcga
    12361 tctctaaaga aacagcgcaa gctttgatta ttggacatga actatttcac cacatcgaag
    12421 cttctgttaa gggaatttat acgcaaagcg aaaaaattgt gctgtggcgg ttgccttttt
    12481 atacacatca atcaaacatt cgtgcattga gcgagatagc agcgatgagt ttttcaaaag
    12541 agatgaatca aagcgatttt tcgccttatg tattagaggc agtcttactt tggccatata
    12601 atgaaattcg tagccaaggg atcttagaag aaataaaaaa aatagaaaaa agatgtacgg
    12661 agtatgattt agattacaaa taaagagact gtgacaaaag tcgtgtcaca gtcctttttt
    12721 tccgaataaa cgatgttctc ctgtcaactc ctcggtcaca agtcaaaata ttcaacaaac
    12781 atgagaagct gttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga
    12841 cctcttggaa aacggtgttc aaaagcttgc atctgcggta ataagtccag ccaagttaaa
    12901 aacataagaa gctattttta cttggctgtt cttattactt gatgcagggc agctttttca
    12961 caacctctca ataaacggtg tgcaatcagc tgaccctcgg tattagctca aaaaaaacgg
    13021 caaaatatga ggaactattt tgccgtttct ttctctaata ctcaggtcaa aacgctgatt
    13081 ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc aaaataatcg gggtaattat
    13141 gaatcacgta ttcttgctcc tcgataatca tatgtaaatg catcgtgcaa agaaatccga
    13201 cttcatcaaa gttatgatca atcattgatt gaagcattgt ctgatgttca tctaatactc
    13261 gaccccagtc taattctgaa atcgtcaaac gtaaccatct gaaacggttc aagtgcgtac
    13321 tataactttc tagccaatcc caaatttctc gttttccagc aatttcataa caagttcggt
    13381 gaaaatcagt atccaaatca aaaaaagctt ttttattttg agcgattact gcctgatcgg
    13441 tttgttctag tattttttct agtttagctt gatcttgctt ggtcattttc gcactgcatt
    13501 cttgcaggat cggtttttcc aacttttccc gcgtataacg ggctaatgaa gcagatcgga
    13561 tatcgatttt tgaaacataa gtgccggact gtggaatggt ttttacaagc tcttgattgc
    13621 gcagttggat cagtgcttct cgaatcggtg tacgcccgat agctagttgt tcttctaaca
    13681 ctttatcgga gattttactt ccaggtgcta actcggaata aatgatttgt ttacgtatag
    13741 attcataagc ttgctgttgc aaattattga taaccattta accttccttt ctatgcagat
    13801 ataatctatt ataacataaa tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa
    13861 aaaaagccct gataggcatg cctattagag cctcgaaaag aaaaatattg aatcaatctg
    13921 gtcgtacctc ttgtatcggt ttgacacgac cgccgattct tttagcaaaa gcttcggatt
    13981 cttttttgtc tttgaaaatg agcagattgt ctaagttttt ttcccgagtg cgacagccag
    14041 ttttatcgat gtaaccgtgc attactttaa cgacatacat ggggatcacc tgcttttttc
    14101 ttttcattat acgaactact cgacacaata caagaaaaat gcttagacgg tctttattaa
    14161 gaatttacat catccaaaga agcgtacgct tggaataact gatctaactc cggattgact
    14221 ttgctcagct tcactaatcg ttgcgaatca gaaagtaaaa aacagtgttt actactgcca
    14281 gtagttttta attttttgtc ttcagttccc acaatttcgt agctaaaatc caaccgtgtc
    14341 cccgtatact tttggatagt tggatggatg ctcacttttt ctccgtaatg gatcatttct
    14401 ttatagctgc aatttacttc cagaacgggg atgatgatcc cagattcttc gattttttta
    14461 tagggaaacc ctagatgtac caataaggct acccgagcct cttcaaacca gcggatataa
    14521 ttgctgtgat gaataatccc catttgatct gtttcataat aaaaaggttc tcgcacataa
    14581 cctgtaaatt tctccatgac tcatgcctcg ttttccattg attttttaca aaaataattc
    14641 atccatttcg gaagtgcttc gctgatttgt gtcggcaaaa caatatattg ttcttttgcc
    14701 agctggtctc caatgaaact atgaagataa atagctgctg caatcgtttg gtcattttga
    14761 ggaaactggg ctaaaaatcc tgtgatcatc ccagctaaag tgtcacccat gccgcccgtt
    14821 gccatagcag cagaacctaa aggattggtg tagaagtgct ggtgaccata aatttttgtc
    14881 tcatgacttt tcaaaacgag cacagcaccg atttcctgtt gcttcttttt gttttcttta
    14941 tcccgttgtt cttgtatcgg tagagaactc aaccgttgcc actccatttg atgaggtgta
    15001 agtacagttt gttcgggata gggtagcaaa agatgattag cagcaagtaa acttaaagca
    15061 gagccatcaa tcaccagcca ttgttctttt gattggtttt ctagtacttg tttgaaacgt
    15121 tccaaactct tagaagaagt acccaatccg ggaccaatca agagtacatc tgcttgcttt
    15181 attacggaaa gaaactggtc agtatccgtc caaactacaa tcattgcttc tggcagatga
    15241 ctgtgaagag cactccgatt tttttcatca gttactacag tcgtgagacc cgctccgctg
    15301 tgtacggcag ctaaagcact cataatgatt gctccgccat attgttcatt gccgccaatc
    15361 aaaacgatac gtccaaatgt tcctttatgt gaaatttttg gacgaggtgt gatgactctt
    15421 ttcactagtt gttcgtttat cagcatctaa aaacctcctt ttactcatta tagcgtatat
    15481 tttttttaga gaaaaacaga taagaaaagg aattgtcaga ttttttgaac tatttttttc
    15541 tgtaatttgt ggtatcatga acacgaagac ttaaataagt ggaggattaa ctttatgaca
    15601 attgattggc aaaaagaagt agaagcacgt aaagaagact tgcttgaaga cttgaaaaat
    15661 cttttacgtg tcaacagcga gcgcgatgac tcaaaagtaa caccagatgc accatttgga
    15721 cctggacctc gcgacgcatt gaaacatatg ttagcttatg gcgaacgtga tggatttaaa
    15781 gtaaaaaatg tcgataacta tgcagggcat attgatcttg gcgaaggaga cgaaacgcta
    15841 gggatatttg gacacatgga tgtcgttcct gcaggagatg gctgggacac agatccttat
    15901 gaaccagtaa tcaaggatgg aaagatcttt gcacgcggat caagcgatga caaaggtcca
    15961 agtatggcag cttactatgc gatgaagatc attaaagaac ttgacttgaa actatcaaaa
    16021 aaagttcgct tcgtagtcgg cagcgatgaa gaaagcggct ggggcgatat ggcttattat
    16081 ttcgaacatg aagaagaacc agattttggt ttctcaccag atgctgaatt cccaatcatc
    16141 aatggtgaaa aaggaaatgt ttctttagct ttacgcttca aaggcgacaa tgcaggagat
    16201 tatgtcttga aatcttttgt ttccggactt cgcgaaaata tggtgccagg aacagcaact
    16261 gcagcacttc aagtaccaag tgcagatgca gctatcgcga tggaagaagc attttaccaa
    16321 ttcatcgaag caaatccagt aagcggaacg attgaagcgg acaatacgta tgtaaaaatc
    16381 gaattagttg gtaaaggcgc acatggtgca agcccacaat ctggtatcaa tgcaggttct
    16441 ttcttggcat tgttcttaga taactatgaa ttcctaggat ctgctaaaca atttatccat
    16501 gtagcagctg cttatgtcca tgaagatttt tatggagaaa aactaggagt cgcttatgaa
    16561 gatgaaaaaa tgggcaaatt gacaatgaat gctggactat ttgcttttga agaaaatggt
    16621 acagaggaag cgaacttcat caatatgaat ttccgttttc caaaaggtgt aacagtagac
    16681 ggattgcaaa gtgacatcga acaaacagtg gggcaagaag gcgcaacagt tactcgaggt
    16741 gctcgcgtaa tggaaccaca ttacgtacca atggatgatc cattggtggc tacattattg
    16801 caagtctatg aagaccacac tggagaaaaa ggctacgaac aaatcatcgg tggcggaact
    16861 tacggacgtt tgttgaaacg tggcgttgct tatggagcga tgttcccagg atatactgat
    16921 acaatgcatc aagcaaacga atttatgagt cttgatgact tgttcagagc aacagcaatc
    16981 tatgcagatg ccatctatcg tttggcgaaa taattaatta aaaagaggat caagaattgc
    17041 aaattatttt tgcagtcttg atcctttttt tactttctac tgtggtattc atgataaaca
    17101 tgatatagat tcaattcata ttctttctta gcgttcgtga cgactgtatc caaaatcaac
    17161 cctgttacta atgaaagaag tgctaaagtc atcaatccag tacttaaaat cgcagaaggg
    17221 atgcgtgtaa taaagcttgt cagcatatat tcgcgaatca caggaatccc cgtgaccaat
    17281 ccaaataaaa agaagaagat ggtccaaatc ccaaagaaaa gcaaaggctt ataatcttta
    17341 aacattttaa taatcatcat gatcactttg aatccgtcag agaatgtatt caattttgat
    17401 tcgctgcctt caggacgatc acgataatcg attgggattt caacgaactt gaatttttta
    17461 tctaaagcat ggatcgtcaa ttccgtttcg atttgaaaac cagaactcat aataggaaaa
    17521 cttttgacaa agatacgatt gaatcctcga tagccggtca tgacatcaag aatttttgtt
    17581 ttatacattc tagtaattgt atttctaaca aggttatttc caaaatcgtg gaaaggtctc
    17641 ttattctcat caaagtaggt tccatttgat aggcgatcac caatgaccat atctgcttca
    17701 ccagaacgta atttctcgag caaaccatgt acagcttctg caggataagt atcatcgcca
    17761 tctaccatta aataatagtc ggcatcgata tcaaagaaca tttggcggat cacatttcct
    17821 tttccttgac gaggttcttt ttttacgatt gctccacctt gaacggccaa ttcgtatgtt
    17881 cgatcggtcg aattattatc atagacataa atatctgctt ctggtaattc tcgtttaaag
    17941 tctgcaatga ctgttgaaat tgttgcttcc tcattgtaac aaggaatcag aacagcaatt
    18001 ttttcttgtt gttccatttt tcactctcct taactataga aaacaaaagc taaattaaaa
    18061 atacctcata atcaaaggaa atgctagttt ttcccgctaa atatcattta tttaaaaaga
    18121 aaatactgga atatatgttt ggaaagctct atctaaaaaa ccacccacta caaaagaagg
    18181 acatgatacc tctcttttga atagtgggtg cgtattcgtt ctctcacttt tttgacgtat
    18241 taatctgttg cacctttttt tcttgaaatt tctaatacgg ttcctgtatg agcatctgct
    18301 aaaaattcat aataaacagt ttcgccatct tctattcgag aaagacctcc ccgataagca
    18361 ctcgaccgaa cggcgaattt cctaataggt tccttttcaa aagagatcca agaaccttcg
    18421 attgcacctt cttttagaaa agatgacttg atattttcaa gtacttggtc tgctgacaat
    18481 acttttttgc gatgatacca aatcgttgaa gctaccccgc tcaataatcc tagaccgatt
    18541 ccaactgcta acccgccttt aaaatagctt agttcgcttt cttcatacat gtggacatac
    18601 ctccttcttc tcacttgtta ctccttgtgc ttattttacc acactctcat attttctttg
    18661 aaataaataa ccgctaccag aaaaaataag ctataataaa tacgatcggt ctgcagaaaa
    18721 aatcatattt tatccgcaga tcaatataag tctgagagga gtttagtctg aaatttagac
    18781 gaatagcata tggaagaaaa aacatttcaa cgaatcaaag agttaacaga attacaagga
    18841 acaagcggtt ttgaacaaga tgttcgcgct tatatggaaa gtcatatgga accgctggta
    18901 gatgagttac agttagatgg tttgggcggc atttttggtc tgcgccatca tgaagaagcg
    18961 gatgcacccc gtgtaatggt cgcagcacat atggatgaag tcggattcat gctgacacaa
    19021 atccaagaaa acgggctttt taaagtcgta cctctaggcg gctggaatcc ttacgttgtt
    19081 tctgctcagc gttttacact taaaacctct actggtaaga attatccatg tatttcttct
    19141 tccgttcccc cacatttatt gcgaggaaca agcggacaaa aatcagtaga agtaaccgat
    19201 attttgttcg atgctgggtt tgaatcaaga gaagaggcaa tgagttacgg tgtccttcca
    19261 ggagacacaa tcgttcctta cgctgaaacg atcaaaacag ccaacggaaa gaatattatc
    19321 agtaaatcat gggacaaccg ctatggctgt acaatggtat tagaagcttt agaagcgttg
    19381 caaaatgaaa cattaggtca tacattgatt gcaggcgcga atgtccagga agaagtaggc
    19441 ttgcgtggtt ctaaagcttc tgtcaataaa ttcaagccag atctgttctt tgcagtagac
    19501 tgctctgcag cagatgatac agtgacgaaa aatggcacat tcggacattt aggagaaggt
    19561 acattgatgc gtatccaaga tccaggtttg atcatgcttc ctcgtttgcg cgagtacttg
    19621 ctggatatcg ctgaaacaaa caatatccct tatcaatact ttgtttcaaa aggcggtaca
    19681 gatgcaggtg ctgctcatac acaaaacgaa ggaatcccaa gtacagtgat tggcgtagtg
    19741 ggacgttata tccatacgca tcaaacgatg ttcagtattc gcgattttga agctgcacga
    19801 gaaatgttga ttcaaacact gaaaggtttg gataaatcaa cagtgaatac gatcgtatac
    19861 ggaaaataat gcgatagtga aactataaaa tctggcaaaa gattgctttt tgccagattt
    19921 tttttataaa aagaggatca atgtttgtta gttcgaaaga aaggtgtata atttaattga
    19981 aaaatacata aacagaaagt agaggacaaa gaatgattat cccaaatagt ttagaagaat
    20041 tagcaggcta cgtagaaact ggaaagagtg tatttttctt tactgccggc tggtgcggag
    20101 actgccgttt tatcaaaccg caaatgcctg aaattgaaaa tgacttttct gattggcaat
    20161 ttattgaagt agaccgtgac aagtacatag atgtagccgc tgaatggaac atttttggta
    20221 tcccaagttt tgtcgtgatc caagacggaa aagaattagg tcgtctagtg aataaagatc
    20281 gtaaaacaaa acaggaaatc gaatcgttcc tagaaagtgt aacaaaaggc tgacatctgt
    20341 taggaggggt caagatgaat caagaatata agaatatttt agtaggtatc gacggtagtg
    20401 aacaagcact tgctgctttc aaaaaagcgg tagaagtggc tcgccgtaat aatgggaaag
    20461 tatatgtagc aaacgtaatc gatcagcaat tttataactt tatgggctat gcacctattg
    20521 atcaaaatct agtcgatcag caaactgaaa cagccaagga aatgatcgaa gaatgtaaag
    20581 aatatgggaa atctgttgat tacacaaata tagaaggaat tattgcatat ggttctgttc
    20641 gtgaatccat ggcagtcaaa ttgccaaaaa aatatgagat cgatctaatc atggttgggc
    20701 agtcaggtct aaatgcagtc gaacgcttta tgacaggaag tgtagccagc tacgtgatac
    20761 gtcattcacc ttgtgatgtc ttgatcgttt ctgacgaaga agaacagcca agtaattaag
    20821 caagtggaac ttgtacgacc tttttaatca attaactaaa acagtaaagg gactgtgaca
    20881 aaagtcttgt cacagtccct tgttacgaat aaatggtgtt ccagaagagg cttcttcgga
    20941 aataaagcat gaaaaaaatt tcatgcagtg aatgcaattt tttgttaaga taaatctcag
    21001 caaaggataa tggaggaaag aacatgattt ttgcttataa caaagcacac gtcggagaca
    21061 ccttgatggt tattatagcc gacgataaag gaacagaaaa cactgttgaa cgcaaatgga
    21121 atgttgctcg gatcaaagat gaacaaggtc aagttgttgc ttggaacttt ttccatatct
    21181 ccgatcatct aacaatcgaa gggaacggcc aagtaagctt aacagatgag gaattagcga
    21241 aattgaatcg attgatcaaa gaagcaggat tcgaagaaca gcttgaagct gatcatgaac
    21301 cgaagatcgt tgtcgggtac gtaaaaacat gcgtcccaca tcctgattct gaccatttat
    21361 cgattactga gacagaagta gacaatggta cagtattgca aatcgtttgc ggtgcaccca
    21421 atatcgaagc tggacaaaaa gttgtcgttg caaaaccagg agctatgatg ccagatggaa
    21481 tgatgatctg gccaggaact ttgcgagggg tagatagcta tggaatgatc tgttccgctc
    21541 gtgaattgca tttgccaaat gctccagcaa aaaaaggaat cttagtattg cctgaggatg
    21601 ccgtagtcgg agaagcattt ccaaaataaa gaggttgtga caagtctttg tcacaacctc
    21661 ttttttatta attttcacta ttttgatttt gttttaatgc cgattgatcg atcgtcaatt
    21721 caacagtagt tgttttttct ttcgagcctt catagtaagt gattttgaca gaatctccca
    21781 cttttttctt atacaaagca gattgaagtt ctacaccaga agaaacttct ttgtcatcga
    21841 tcttagtaat gacgtcgtat tgtttcaatc cagctttttc agccggtgta gctgtttgga
    21901 catttgtcac gatgacaccg ttagttacag atgaaggaat cttcaagatt tgttcttgtt
    21961 gctgagtaga aacggcagat aagtcgacca ttgtgatacc tagagctgga cgtgtcactt
    22021 ttccgtcttt ttccagttga ttgatgatat tcactacatc gttacttgga atagcaaatc
    22081 ccattccttc tacgcttaca ttagaagtag attcagaagt acttgcgatc ttacttgagt
    22141 tgataccgat aacttgtcct tcgatattaa ctaaaggacc accagaattt ccagggttga
    22201 ttgctgcatc tgtttggatc gcattgatat tgactgtttc gttcgattca ttcgtacttg
    22261 tcacttgtcg gttcaaggaa gaaatgattc ctgaagtgac agaatttgca tattcagatc
    22321 ctagtgggga accaatcgca atcgcaggct caccaacctt cagagcactt gagtcaccaa
    22381 aagaggcaac tgtttcgact ttgtctgaat tgatcttcaa caccgctaaa tctgaataag
    22441 catctgttcc aactaattca gcttttacct tcgtaccgtc tttcatcaag acttcaagac
    22501 cttgctggcc atctactacg tgattattcg tgactacata agcagtattc ccgtcttttt
    22561 tgtagatcac gccgcttcct tcactataag cttccaaatt actatcatct gagctgctac
    22621 tttcttcttg ctgcccgaat agtccgccaa aaccgcttga ttggttttga ctttgtaagt
    22681 tgataacaga aacgacagcg tcttgaactt tatctaccgc tttggtgata tcagaatcta
    22741 cattgacttt gacattttcc acaactgttt caccggcaga attctgattg ccgcttgtag
    22801 cagtagaact attgttgcca gtgcccatcg ctgcataaaa aatgccagct gtcagtagtc
    22861 ctccaacgat ccctccaaca agccccagac ctaacttacg ccagaggcta ttatttttac
    22921 tttttttcat ctttggtgtc acattttttc tatccatgtg ttcctcctcc aattacattc
    22981 atttatctac tgttagtata gtcttatttg ttgactttag tctactttga gactgtgaac
    23041 tttttatgaa aaactttagg aaaaatcatt gttttcagat gaaaatattt gattttcttt
    23101 ttccacagat actgtgtaaa actacgcgga taggattgga aaaataaggg ataaaacata
    23161 tccacagcct gtgtataaag tggataaatt cttataaaac gtatgtttgc ctaatgaatg
    23221 tgatgaaata aggtaaaata acactgtttt ctgtggataa acctgtggat gttgtgcata
    23281 actgttgggg aaatcttttt aacaaaaata gccaaaataa acatatagac gaaaaaattg
    23341 attataataa acaaagtgag catgaagacc aaaggagaga aaacatgaat atcaaaatta
    23401 tctcagtagg aaaattaaaa gaaaaatacc tgatccaagg tatcaatgag tatgtcaaac
    23461 ggctgaatgc ctatgcaaaa atcgaattga tcgaagttcc tgacgaaaaa gccccggaga
    23521 atttaagcga agcgcaaatg agacaagtaa aagaaaaaga aggggaacgg atcttagcga
    23581 aaatcaaaga acaagaatac gtctatgcct tagcaattga agggaaaaat cctacaagtg
    23641 aatcttttgc taaacaaatc gaccaattag gtatccaagg aaaaagccac cttgtttttg
    23701 ttatcggcgg ctctttagga ctcagcgaag ccgtcatgaa aagaagcaat gctcagatct
    23761 cttttggaaa aatgacgtat ccacatcaat tgatgcgtct gatccttgtc gagcaaatct
    23821 accgtgcctt ccggatcaat gcgggggcgc cttatcataa gtgatgcggt ttttatcatt
    23881 gatcctaaaa atcttagcaa gacaaaaagt acgtaagaaa gtaaagacta aatacttcgg
    23941 aggaacaaat gaataacaat gataggttat tgcgattgcg ttatgcgatt gatttgaaag
    24001 atacggattt gattaaagct tttgagctag gcggtgtacc actgactaaa gaagaagctc
    24061 aggcggtact gaccaaagta caggataaga acaaagataa tgcagaaaat aatgtgtatg
    24121 aaaaaaccat caacaaccaa gtttttgacg cattcctaaa tggcctgatt ctcttggcgc
    24181 gtggtccgca aaaagataag ccgatagtag acacgccgca aaaaagcaag gaaatcaaat
    24241 atatcaacaa tgtgttgcta aaaaaattaa aaatcgcctt gtctatgacc actgatgata
    24301 ttttagacgt ttttgctgag gcagaaatct atccatcaaa aggcgaaatc ggggcatttt
    24361 tgagaaaaga aggccaacga aattttaagc cttgcggaga taaatatatg cgtaattttc
    24421 tcaaggggtt aggaatttat aatcggagaa aagtataaag aaatttcatg agaaatcaaa
    24481 acaatggttg ttttgatgag agctagtttt tgtatgagat taaaatatta tcttaatcga
    24541 ggagctggat tggttttatg aatgatgaat ataagaatga tgaagataag atgctttttg
    24601 aagagataga gaatcgatgt aggctcaatt ttgagcttcg gggaaagatg tctttaatac
    24661 aacaaaaaaa gtatttagcg aataaatcag agtttactct gggacatgtt gaaaaattaa
    24721 tctcggattg gattagtagt agaagcgaat ttacaaaaat aaaacagccg attaaatttg
    24781 acatgaaaaa acttttgtta aataagagtg agataggtaa tagagatcaa tatattagag
    24841 ctaaagggca agaaataatt gattcattag gtgaaatgag gtcatacaat tatttgtatg
    24901 taacacatag agctgatgga atggtcatta ctgtggggaa atcttcttct aatgatattt
    24961 ttttagatgg agatcttttt tatcaactca atacaaatca cttgtctggc actgaaaata
    25021 taattttaag gacggaatat ggcaatgaaa tatttgcaaa atatgatgag attttaaaaa
    25081 attatttgga ctgggcttgg ataattccgg ttgagtcagg agatgctaag aaactggaga
    25141 gattattggg cgatgaacta attaataaaa aagtaccaat tttaaattat tattcccata
    25201 gacaataaaa ggctattttt catgataata ctagttataa ctgtaaaaat ggaagaaaat
    25261 ctgggaaaga agtttttaaa taagtgtatt gttcgatttt aataaataat aagcaatacc
    25321 atccgtctgt gttgcgcaac atcgtactac aatgaatatt ttttcacaaa aagaaaatca
    25381 tccatcgaat gaatggaagg ttttagtata cacgaggtta atgccccctc ttttataatc
    25441 agaggagtga ggttttttct atgaatgttt ctattttctt attgctagtc tagtttctct
    25501 tacgatgaaa ttgggattct attccagttt ttattgattt gctgataaaa taacttgaat
    25561 ggtggatata aacatgataa ataagagaag aataacaaaa caaggttgtg aaatgattct
    25621 caaatgaata taatagaaag aaaaaggagg aagaatcaat gaaaatagca attgcaggcg
    25681 ctggcgcaat gggaagtcgc tttgggttaa tgcttcacca aagtgggaac gaagtattat
    25741 taatcgatgg ctgggcagaa catgtccaac aaatcaaaga gcatggatta caagcaaatt
    25801 ttaatggtaa agaggtagaa gcaaaactac caatcgttct tcaatccgaa gtagaaaaag
    25861 aagatcaagt tgatctgatt attctattta ccaaagcgat gcagctggaa aaaatgctgc
    25921 aggatatcca atcattaatc aaaaaagata cagaggtctt atgtctatta aatggtatcg
    25981 ggcatgaaga tattattgag aaatttgtac caatggaaaa tatctatatt ggaaatacca
    26041 tgtggacggc aggtcttgaa ggtcctggtc aggtcaaatt atttggaagt ggttcagtag
    26101 aattacaaaa tttaggtgat ggaaaagaag cagctgcaaa aaaattagca gacaaattgt
    26161 ctgaatcagg attaaatgct catttttctg acaatattca ctattctatt tatcgcaaag
    26221 catgtgtgaa cggaacaatg aacggactat gcacaatctt agacgttaat atggctgagc
    26281 taggaaaaac atcgactgct cataaaatgg tggcgacgat tgtcaatgag tttgccaaag
    26341 tagcagcagt agagaagatt gaactagatg tcccagaagt cattgcacat tgtgaatctt
    26401 gttttgaccc ggaaacaatt gggttacatt atccttcaat gtatcaagac ttgattaaga
    26461 accatcgatt aacagaaatc gattatatca atggcgcaat ttctagaaaa gggaagaaat
    26521 atggtgttgc gacgccttat tgtgatttct tgactgagct tgtccatgca aaagaagata
    26581 gtttgaacgt aaaataaagg agagattgaa tggaaacttc acaaaaaata tcaccgaaaa
    26641 tttttctgaa taaagtcctc gctggtacag caacaggaat cattattgga ttgattccta
    26701 atgcagtatt aggtgccatt ttaaaatatt ttagtgcgta tccctttgcg gtaacgatcg
    26761 cacagttagc agttattttt caactagcaa cacctttgat cattggtggt ttgattgcct
    26821 tacaatttgg atttgatccg atgagaatga tggttgtagc cggagcttct tttgttggtt
    26881 ctggtgtagt caaattcaca cctgatttgg gtgaaacggg tgtttacgtt ggtgctggta
    26941 caggcgatat tattaatacg atgctgacag catcgattgc tgtcgggctg attttactga
    27001 ttggaaatag ttttgggtca gtgtcgattg tgttaacacc gattgttgta ggtgtaggtg
    27061 caggtttact tggtttttat ttattccctt ttgtaacagc aattacttct gcaattggat
    27121 cattgattaa taacttcact accttacaac cgctgttaat gtctatcttg attggttgtt
    27181 cctttgcctt tttgattatc tctcctattt caacagtagc aatcggaatg gcaatccaac
    27241 tagatggtgt ttctgctggt gcggctgcga tgggcgtagc tgcaaccact tttgttttag
    27301 tagtgaactc ttggaaggtt aataagtcag gtgtgacaat tgcgattgct ttaggcgcta
    27361 tgaagatgat gatgcctaat ctatttagaa aacctgtgat tcttattcct tgtttagtta
    27421 gtgcagtgat tacagctatt cctgtcgctc tattttctat ttcaggtacg cctgcttctg
    27481 ctggtttcgg cgtggtcgga cttgttggtc cgttggcttc tttagatgca gggctaaatg
    27541 gctttcttgt tgttctttgt tggttagtca tccctgtttt tgcggcacta attactcaat
    27601 ttttatgcga gaaagtattt aaactgtatg atcgagaaga agtcttcaaa tttttaggct
    27661 agtagatttt actaagaaat tttcaatcaa tggatgcgta agtgagatgt caacaacatc
    27721 tgacttacgt ttttttgcct ttttgagtag gagattgatg ctgacaactg aactcttcag
    27781 tgccattcga ttttttaaaa tgtagaagaa actcaccttt atgttattga ttgttcaaaa
    27841 ggcaactaga ataaaaatat gtgttaaaat gagaaaaaat ttgattgttg gatgtatcga
    27901 ataatcacta aaaagtagga gaagaaaatg atttttttgc aaaaatatgg tttttatttt
    27961 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
    28021 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
    28081 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
    28141 ttgtatcacc tatttattgg aacatcaaaa acacttgcaa tacttgctgt tgcaacagtt
    28201 ggtttatatg ggataggtga ttgtattttt actgggttat tcagtatcaa tacgaatgaa
    28261 agctcttgga atctatcgac ttggatccat aatataggtt ctgggctagg ctatgcaggc
    28321 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaagtggtca aggtacgctt
    28381 agccattttt atcttgtttt aacagttatt agtttactaa ttgcaggtct ttacggatta
    28441 gcacggattc cgtcgattag tcagtttgcc ttctttaagc aattgggctt ctttcaacgc
    28501 ttaagttttt tctttaacta tttgccgatg atttgtttaa gtttttttca actacgaaag
    28561 tagaaaaagc agtgtgaagc tgattaagta aaagaatttc tctatgggta accattcgtg
    28621 tggatgtgtt cgactaagaa tagagatgag gagagcgaaa agcaagttag tcttaacttg
    28681 ttatgcgttg tgctaaattt caggtagtca ttttttgaat gaataagaag aaaacttgaa
    28741 agagggtgac ttcatgttag atgtggagca gatggaaata gacctttata atattatttt
    28801 aaatcgaaat acaagagaat gggaaagaac acaatttgaa gaggcaaaag aagacctagc
    28861 aaaaatgtcg ataaagcagg tgatagaaaa actagaggtc aacttgcgtc cgctagcact
    28921 acgagataat ttgacaccag atgcgatgga tttttattta agaatcacag gggagtcagc
    28981 gagtgcaaag gcttatgatt atgccaaaca taagataact tcacccttat accaagaacg
    29041 ggcgattttt gccggtggct gtttctggtg tatggttgag ccttttgaaa caaaaccagg
    29101 tgtaatctca gtgttatctg gttatacagg tggagagacc aaaaaaccaa attacgatca
    29161 agtcagtggt ggatatactg ggcatgttga agctgttgag atcatatttg atacacgtca
    29221 aataacctat gaagagctgt tggagattta ttggcaaatt tcagacccta cagatgcctt
    29281 tggtcaattt caagatcggg ggaaacaata tcgttcaatt ctatttgttg ttaatgaaaa
    29341 gcaaaggcaa ttggctgaaa aatcaagaga acaactgatt caatctaacc gatttagccg
    29401 tcctatcgtt acagagattc gccaagcaaa gactttttgg ccagctgaaa atcatcatca
    29461 acaattttat cagaagcaac ctaaaagata taaaaagatc aagcattcgc atcagcttca
    29521 tttatttttt cagcgacata gatagaggaa ggttgtggcg caaagcgttc agctccgaca
    29581 aaataatgag caactgacaa aaatagcttt tcatattttt ggacagttat gaattatttc
    29641 gaggagttgc tttgagaaca ccgaggaaaa ggttgtggcg caaagcattc agcttcaagt
    29701 cgcaaggaaa caggaggtat gacaagtctt ttgtcacaac tcctgttttt ttactagcct
    29761 ttatttccat gtgaaagaga attcgaattg tagaatcttg atttttctat ggaattttgt
    29821 agagaatcat cttgataatt aatagacacc taatcgaatt ccacattaat gatgaactaa
    29881 attccgtata aaagcgcaaa caataactat aaaacccgt
//
LOCUS       DACDCJ010000028        29140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDCJ010000028 DACDCJ010000000
VERSION     DACDCJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.028"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(2..556)
                     /locus_tag="IV038_001251"
     CDS             complement(2..556)
                     /locus_tag="IV038_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP8341481.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(682..2611)
                     /locus_tag="IV038_001252"
                     /pseudo
     CDS             complement(682..2611)
                     /locus_tag="IV038_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(2917..3633)
                     /locus_tag="IV038_001253"
     CDS             complement(2917..3633)
                     /locus_tag="IV038_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8341482.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3634..5457)
                     /gene="glpO"
                     /locus_tag="IV038_001254"
     CDS             complement(3634..5457)
                     /gene="glpO"
                     /locus_tag="IV038_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP8341483.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5471..6967)
                     /gene="glpK"
                     /locus_tag="IV038_001255"
     CDS             complement(5471..6967)
                     /gene="glpK"
                     /locus_tag="IV038_001255"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP8341484.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7103..8563)
                     /locus_tag="IV038_001256"
     CDS             complement(7103..8563)
                     /locus_tag="IV038_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8341485.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8553..9848)
                     /locus_tag="IV038_001257"
     CDS             complement(8553..9848)
                     /locus_tag="IV038_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8341486.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10180..10857)
                     /locus_tag="IV038_001258"
     CDS             complement(10180..10857)
                     /locus_tag="IV038_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP8341487.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10887..11978)
                     /locus_tag="IV038_001259"
     CDS             complement(10887..11978)
                     /locus_tag="IV038_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP8341488.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12079..12513)
                     /locus_tag="IV038_001260"
     CDS             complement(12079..12513)
                     /locus_tag="IV038_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8341489.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13097..14554)
                     /gene="ascB"
                     /locus_tag="IV038_001261"
     CDS             complement(13097..14554)
                     /gene="ascB"
                     /locus_tag="IV038_001261"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP8341490.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14551..16446)
                     /locus_tag="IV038_001262"
     CDS             complement(14551..16446)
                     /locus_tag="IV038_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8341491.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16504..18279)
                     /locus_tag="IV038_001263"
     CDS             complement(16504..18279)
                     /locus_tag="IV038_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8341492.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLDESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18329..19825)
                     /locus_tag="IV038_001264"
     CDS             complement(18329..19825)
                     /locus_tag="IV038_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8341493.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20019..21641)
                     /locus_tag="IV038_001265"
     CDS             complement(20019..21641)
                     /locus_tag="IV038_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP8341494.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21969..23282)
                     /locus_tag="IV038_001266"
     CDS             complement(21969..23282)
                     /locus_tag="IV038_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8341495.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23527..23751)
                     /locus_tag="IV038_001267"
     CDS             complement(23527..23751)
                     /locus_tag="IV038_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341496.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23771..24139)
                     /locus_tag="IV038_001268"
     CDS             complement(23771..24139)
                     /locus_tag="IV038_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP8341497.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24336..25046)
                     /locus_tag="IV038_001269"
     CDS             complement(24336..25046)
                     /locus_tag="IV038_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341498.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25064..25561)
                     /locus_tag="IV038_001270"
     CDS             complement(25064..25561)
                     /locus_tag="IV038_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8341499.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25598..26050)
                     /locus_tag="IV038_001271"
     CDS             complement(25598..26050)
                     /locus_tag="IV038_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8341500.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26185..26790)
                     /locus_tag="IV038_001272"
     CDS             complement(26185..26790)
                     /locus_tag="IV038_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP8341501.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27240..27860)
                     /locus_tag="IV038_001273"
     CDS             complement(27240..27860)
                     /locus_tag="IV038_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8341502.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27862..28731)
                     /locus_tag="IV038_001274"
     CDS             complement(27862..28731)
                     /locus_tag="IV038_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP8341503.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28754..29116)
                     /locus_tag="IV038_001275"
     CDS             complement(28754..29116)
                     /locus_tag="IV038_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP8341504.1"
                     /translation="MAKEEKALSPEATEKIQSEITQLKQEISTTQDKHKLAKLYEQVG
                     LKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRADAARAGDDQGIDYYMG
                     KMDEMRQIAKQVTIKGNK"
BASE COUNT         8184 a         6328 c         4776 g         9852 t
ORIGIN      
        1 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
       61 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
      121 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
      181 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
      241 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
      301 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
      361 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
      421 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
      481 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
      541 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
      601 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
      661 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
      721 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
      781 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
      841 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
      901 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
      961 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
     1021 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
     1081 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
     1141 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
     1201 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
     1261 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
     1321 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
     1381 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
     1441 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
     1501 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
     1561 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
     1621 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
     1681 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
     1741 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
     1801 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
     1861 atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
     1921 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
     1981 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
     2041 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
     2101 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
     2161 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
     2221 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
     2281 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
     2341 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
     2401 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
     2461 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
     2521 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
     2581 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
     2641 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
     2701 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
     2761 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
     2821 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
     2881 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
     2941 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
     3001 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
     3061 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
     3121 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
     3181 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
     3241 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
     3301 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
     3361 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
     3421 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
     3481 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
     3541 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
     3601 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
     3661 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
     3721 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
     3781 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
     3841 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
     3901 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
     3961 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
     4021 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
     4081 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
     4141 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
     4201 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
     4261 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
     4321 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
     4381 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
     4441 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
     4501 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
     4561 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
     4621 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
     4681 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
     4741 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
     4801 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
     4861 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
     4921 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
     4981 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
     5041 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
     5101 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
     5161 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
     5221 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
     5281 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
     5341 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
     5401 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
     5461 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
     5521 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
     5581 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
     5641 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
     5701 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
     5761 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
     5821 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
     5881 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
     5941 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
     6001 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
     6061 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
     6121 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
     6181 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
     6241 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
     6301 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
     6361 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
     6421 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
     6481 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
     6541 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
     6601 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
     6661 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
     6721 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
     6781 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
     6841 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
     6901 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
     6961 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
     7021 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
     7081 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
     7141 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
     7201 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
     7261 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
     7321 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
     7381 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
     7441 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
     7501 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
     7561 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
     7621 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
     7681 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
     7741 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
     7801 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
     7861 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
     7921 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
     7981 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
     8041 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
     8101 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
     8161 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
     8221 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
     8281 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
     8341 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
     8401 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
     8461 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
     8521 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
     8581 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
     8641 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
     8701 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
     8761 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
     8821 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
     8881 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
     8941 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
     9001 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
     9061 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
     9121 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
     9181 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
     9241 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
     9301 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
     9361 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
     9421 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
     9481 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
     9541 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
     9601 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
     9661 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
     9721 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
     9781 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
     9841 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
     9901 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
     9961 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    10021 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    10081 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    10141 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    10201 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    10261 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    10321 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    10381 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    10441 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    10501 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    10561 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    10621 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    10681 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    10741 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    10801 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    10861 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    10921 gggcttggca aataaagctc tttatatgct tcttcaaaag gtttcgttac aaattcatcc
    10981 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    11041 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    11101 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    11161 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    11221 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    11281 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    11341 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    11401 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    11461 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    11521 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    11581 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    11641 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    11701 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    11761 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    11821 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    11881 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    11941 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    12001 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    12061 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    12121 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    12181 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    12241 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    12301 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    12361 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    12421 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    12481 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    12541 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    12601 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    12661 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    12721 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    12781 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    12841 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    12901 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    12961 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    13021 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    13081 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    13141 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    13201 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    13261 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    13321 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    13381 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    13441 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    13501 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    13561 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    13621 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    13681 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    13741 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    13801 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    13861 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    13921 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    13981 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    14041 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    14101 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    14161 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    14221 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    14281 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    14341 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    14401 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    14461 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    14521 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    14581 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    14641 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    14701 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    14761 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    14821 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    14881 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    14941 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    15001 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    15061 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    15121 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    15181 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    15241 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    15301 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    15361 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    15421 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    15481 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    15541 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    15601 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    15661 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    15721 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    15781 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    15841 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    15901 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    15961 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    16021 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    16081 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    16141 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    16201 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    16261 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    16321 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    16381 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    16441 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    16501 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    16561 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    16621 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    16681 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    16741 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    16801 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    16861 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    16921 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    16981 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    17041 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    17101 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcatctaa
    17161 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    17221 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    17281 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    17341 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    17401 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    17461 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    17521 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    17581 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    17641 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    17701 aattgtactg atttgttgag gcaaccttgc gtgttggatc accgtttctg ttccaacaag
    17761 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    17821 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    17881 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    17941 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    18001 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    18061 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    18121 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    18181 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    18241 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    18301 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    18361 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    18421 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    18481 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    18541 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    18601 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    18661 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    18721 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    18781 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    18841 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    18901 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    18961 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    19021 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    19081 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    19141 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    19201 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    19261 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    19321 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    19381 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    19441 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    19501 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    19561 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    19621 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    19681 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    19741 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    19801 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    19861 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    19921 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    19981 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    20041 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    20101 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
    20161 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    20221 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    20281 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    20341 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    20401 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
    20461 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    20521 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    20581 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    20641 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    20701 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    20761 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    20821 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    20881 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    20941 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    21001 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    21061 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    21121 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    21181 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    21241 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    21301 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    21361 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    21421 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    21481 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    21541 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    21601 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    21661 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    21721 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    21781 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    21841 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    21901 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    21961 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    22021 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    22081 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    22141 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    22201 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    22261 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    22321 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    22381 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    22441 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    22501 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    22561 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    22621 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    22681 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    22741 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    22801 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    22861 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    22921 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    22981 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    23041 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    23101 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    23161 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    23221 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    23281 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    23341 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    23401 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    23461 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    23521 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    23581 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    23641 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    23701 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    23761 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    23821 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    23881 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    23941 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    24001 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    24061 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    24121 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    24181 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    24241 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaaa
    24301 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
    24361 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
    24421 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
    24481 accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca gttgagacag
    24541 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
    24601 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
    24661 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
    24721 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
    24781 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
    24841 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
    24901 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
    24961 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
    25021 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
    25081 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
    25141 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
    25201 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
    25261 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
    25321 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
    25381 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
    25441 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
    25501 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
    25561 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
    25621 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
    25681 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
    25741 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
    25801 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
    25861 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
    25921 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
    25981 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
    26041 tttcagccat ttcatcccac ctttattttg tttgtaagac tagttcacct ctttttgttg
    26101 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
    26161 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
    26221 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
    26281 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
    26341 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
    26401 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
    26461 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
    26521 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
    26581 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
    26641 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
    26701 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
    26761 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
    26821 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
    26881 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
    26941 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
    27001 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
    27061 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
    27121 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
    27181 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
    27241 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
    27301 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
    27361 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
    27421 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
    27481 cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag gcgaatcgtg
    27541 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
    27601 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
    27661 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
    27721 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
    27781 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
    27841 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
    27901 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
    27961 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
    28021 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
    28081 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
    28141 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
    28201 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
    28261 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
    28321 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
    28381 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
    28441 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
    28501 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
    28561 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
    28621 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
    28681 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
    28741 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
    28801 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
    28861 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
    28921 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
    28981 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
    29041 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
    29101 tttcttcttt tgccagaact tttttcggtt ctttttcttt
//
LOCUS       DACDCJ010000029        29104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDCJ010000029 DACDCJ010000000
VERSION     DACDCJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.029"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1031)
                     /locus_tag="IV038_001276"
     CDS             complement(<1..1031)
                     /locus_tag="IV038_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8341505.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="IV038_001277"
     CDS             complement(1113..1802)
                     /locus_tag="IV038_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8341506.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IV038_001278"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="IV038_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP8341507.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="IV038_001279"
     CDS             complement(2329..2832)
                     /locus_tag="IV038_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP8341508.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IV038_001280"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="IV038_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP8341509.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IV038_001281"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="IV038_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP8341510.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="IV038_001282"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="IV038_001282"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP8341511.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="IV038_001283"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="IV038_001283"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP8341512.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="IV038_001284"
     CDS             complement(6444..7463)
                     /locus_tag="IV038_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP8341513.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8523)
                     /locus_tag="IV038_001285"
     CDS             complement(7636..8523)
                     /locus_tag="IV038_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8341514.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IV038_001286"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="IV038_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP8341515.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IV038_001287"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="IV038_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP8341516.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IV038_001288"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="IV038_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8341517.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="IV038_001289"
     CDS             9754..10398
                     /locus_tag="IV038_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8341518.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="IV038_001290"
     CDS             complement(10454..10957)
                     /locus_tag="IV038_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8341519.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="IV038_001291"
     CDS             11554..12519
                     /locus_tag="IV038_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8341520.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="IV038_001292"
     CDS             complement(12618..13790)
                     /locus_tag="IV038_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341521.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="IV038_001293"
     CDS             complement(13777..14922)
                     /locus_tag="IV038_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341522.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="IV038_001294"
     CDS             complement(14936..15868)
                     /locus_tag="IV038_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341523.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="IV038_001295"
     CDS             15985..17064
                     /locus_tag="IV038_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8341524.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="IV038_001296"
     CDS             17061..17690
                     /locus_tag="IV038_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8341525.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="IV038_001297"
     CDS             complement(17800..18027)
                     /locus_tag="IV038_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8341526.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="IV038_001298"
     CDS             complement(18130..20718)
                     /locus_tag="IV038_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP8341527.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="IV038_001299"
     CDS             complement(20738..21397)
                     /locus_tag="IV038_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8341528.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IV038_001300"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="IV038_001300"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP8341529.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="IV038_001301"
     CDS             complement(22170..23036)
                     /locus_tag="IV038_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8341530.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="IV038_001302"
     CDS             23145..23786
                     /locus_tag="IV038_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP8341531.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IV038_001303"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="IV038_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP8341532.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="IV038_001304"
     CDS             complement(25227..26126)
                     /locus_tag="IV038_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP8341533.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="IV038_001305"
     CDS             complement(26128..26925)
                     /locus_tag="IV038_001305"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP8341534.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="IV038_001306"
     CDS             complement(26903..27607)
                     /locus_tag="IV038_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP8341535.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="IV038_001307"
     CDS             27738..28313
                     /locus_tag="IV038_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP8341536.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..>29104
                     /locus_tag="IV038_001308"
     CDS             28431..>29104
                     /locus_tag="IV038_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP8341537.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8562 a         6167 c         5233 g         9142 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ttaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt agtggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaa
//
LOCUS       DACDCJ010000030        29033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDCJ010000030 DACDCJ010000000
VERSION     DACDCJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.030"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            107..514
                     /locus_tag="IV038_001309"
     CDS             107..514
                     /locus_tag="IV038_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341538.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            528..929
                     /locus_tag="IV038_001310"
     CDS             528..929
                     /locus_tag="IV038_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341539.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            931..1302
                     /locus_tag="IV038_001311"
     CDS             931..1302
                     /locus_tag="IV038_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341540.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1338..1640)
                     /locus_tag="IV038_001312"
     CDS             complement(1338..1640)
                     /locus_tag="IV038_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341541.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1889..2089)
                     /locus_tag="IV038_001313"
     CDS             complement(1889..2089)
                     /locus_tag="IV038_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8341542.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2394..3626)
                     /locus_tag="IV038_001314"
     CDS             complement(2394..3626)
                     /locus_tag="IV038_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP8341543.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3880..4449
                     /locus_tag="IV038_001315"
     CDS             3880..4449
                     /locus_tag="IV038_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8341544.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4627..5067)
                     /locus_tag="IV038_001316"
     CDS             complement(4627..5067)
                     /locus_tag="IV038_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP8341545.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5225..5989
                     /gene="map"
                     /locus_tag="IV038_001317"
     CDS             5225..5989
                     /gene="map"
                     /locus_tag="IV038_001317"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP8341546.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6021..6944
                     /locus_tag="IV038_001318"
     CDS             6021..6944
                     /locus_tag="IV038_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP8341547.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7020..8159
                     /locus_tag="IV038_001319"
     CDS             7020..8159
                     /locus_tag="IV038_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP8341548.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8152..8952
                     /locus_tag="IV038_001320"
     CDS             8152..8952
                     /locus_tag="IV038_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8341549.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8952..9779
                     /locus_tag="IV038_001321"
     CDS             8952..9779
                     /locus_tag="IV038_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8341550.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9757..10491
                     /locus_tag="IV038_001322"
     CDS             9757..10491
                     /locus_tag="IV038_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8341551.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10591..11457
                     /gene="rfbA"
                     /locus_tag="IV038_001323"
     CDS             10591..11457
                     /gene="rfbA"
                     /locus_tag="IV038_001323"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP8341552.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11471..12043
                     /gene="rfbC"
                     /locus_tag="IV038_001324"
     CDS             11471..12043
                     /gene="rfbC"
                     /locus_tag="IV038_001324"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP8341553.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12065..13093
                     /gene="rfbB"
                     /locus_tag="IV038_001325"
     CDS             12065..13093
                     /gene="rfbB"
                     /locus_tag="IV038_001325"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP8341554.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13191..14042
                     /gene="rfbD"
                     /locus_tag="IV038_001326"
     CDS             13191..14042
                     /gene="rfbD"
                     /locus_tag="IV038_001326"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP8341555.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14077..16110
                     /locus_tag="IV038_001327"
     CDS             14077..16110
                     /locus_tag="IV038_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341556.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16154..17434
                     /locus_tag="IV038_001328"
     CDS             16154..17434
                     /locus_tag="IV038_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP8341557.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17644..18450
                     /locus_tag="IV038_001329"
     CDS             17644..18450
                     /locus_tag="IV038_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341558.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18462..19682
                     /locus_tag="IV038_001330"
     CDS             18462..19682
                     /locus_tag="IV038_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341559.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19672..21258
                     /locus_tag="IV038_001331"
     CDS             19672..21258
                     /locus_tag="IV038_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8341560.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21297..23435
                     /locus_tag="IV038_001332"
     CDS             21297..23435
                     /locus_tag="IV038_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8341561.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(23803..24786)
                     /locus_tag="IV038_001333"
     CDS             complement(23803..24786)
                     /locus_tag="IV038_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP8341562.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            25164..26555
                     /locus_tag="IV038_001334"
     CDS             25164..26555
                     /locus_tag="IV038_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8341563.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            26571..27611
                     /locus_tag="IV038_001335"
     CDS             26571..27611
                     /locus_tag="IV038_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP8341564.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRKIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            27630..28715
                     /locus_tag="IV038_001336"
     CDS             27630..28715
                     /locus_tag="IV038_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP8341565.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            28748..>29033
                     /locus_tag="IV038_001337"
     CDS             28748..>29033
                     /locus_tag="IV038_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP8341566.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSG"
BASE COUNT         9419 a         4767 c         5810 g         9037 t
ORIGIN      
        1 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
       61 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      121 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      181 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      241 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      301 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      361 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      421 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      481 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      541 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      601 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      661 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      721 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      781 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
      841 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
      901 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
      961 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1021 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1081 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1141 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1201 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1261 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1321 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1381 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1441 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1501 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1561 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1621 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1681 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1741 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1801 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     1861 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     1921 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     1981 tagatgcatg cgtatgtctt agtccatcaa taaacagtcg aagagaaaat agggaaaaat
     2041 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaataga
     2101 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccaacgaaag atatatcgtt
     2161 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2221 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2281 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2341 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2401 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2461 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2521 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2581 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2641 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2701 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact
     2761 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     2821 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     2881 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     2941 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca
     3001 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3061 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3121 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3181 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3241 agtgctttgc cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3301 gcatcgggat cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc
     3361 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3421 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3481 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3541 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3601 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3661 ctattagcat gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga
     3721 ggttattttt ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg
     3781 aagaaaatca aaaactaatt ttgcatatca aaaaaacatg ttacaatgaa attaatcaat
     3841 ggtggaagcg ttataaaaaa attaaggaga ttaaaaaata tggcaagaaa aaagacgatt
     3901 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     3961 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     4021 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     4081 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     4141 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     4201 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     4261 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     4321 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     4381 attgaaaaga tgatccaaga cagtatagat gctatcttga aattcaaaga accaatcgaa
     4441 atcgattgaa tatactcaaa agttccacaa atcagtaggt aacaagagat gtgtcaaaat
     4501 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     4561 gaatgcaatg aataaaagag ccatacagat tgaataaatc tggatggctc ttttattgca
     4621 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     4681 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     4741 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     4801 tccgcagaca ccgtaaattt ttcccaatag atccaattct tgcaagtctt cataaaagtc
     4861 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     4921 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     4981 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     5041 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     5101 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
     5161 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     5221 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     5281 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     5341 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     5401 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     5461 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     5521 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     5581 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     5641 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     5701 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     5761 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     5821 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     5881 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     5941 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     6001 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     6061 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     6121 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     6181 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     6241 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     6301 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     6361 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     6421 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     6481 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     6541 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     6601 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     6661 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     6721 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     6781 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     6841 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     6901 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     6961 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     7021 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     7081 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     7141 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     7201 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     7261 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     7321 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     7381 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     7441 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     7501 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     7561 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     7621 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     7681 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     7741 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     7801 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     7861 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     7921 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     7981 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     8041 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     8101 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     8161 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     8221 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     8281 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     8341 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     8401 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     8461 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     8521 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     8581 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     8641 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     8701 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     8761 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     8821 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     8881 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     8941 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     9001 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     9061 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     9121 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     9181 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     9241 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     9301 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     9361 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     9421 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     9481 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     9541 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     9601 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     9661 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     9721 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     9781 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
     9841 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
     9901 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
     9961 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
    10021 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
    10081 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
    10141 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
    10201 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
    10261 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
    10321 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
    10381 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
    10441 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
    10501 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
    10561 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
    10621 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
    10681 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
    10741 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
    10801 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
    10861 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
    10921 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
    10981 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
    11041 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
    11101 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
    11161 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
    11221 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
    11281 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
    11341 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
    11401 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
    11461 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
    11521 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
    11581 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    11641 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    11701 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    11761 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    11821 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    11881 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    11941 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    12001 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    12061 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    12121 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    12181 aggaaacaaa gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    12241 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    12301 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    12361 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    12421 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    12481 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    12541 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    12601 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    12661 tcctcgccaa gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    12721 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    12781 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    12841 cgtgatggaa ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa
    12901 cgaccgcgca gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct
    12961 aggttggcag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    13021 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    13081 aaatggacaa taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag
    13141 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat
    13201 taactggagg aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc
    13261 tgccatatgt ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg
    13321 cttatattac agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag
    13381 ataaagcaga agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa
    13441 atgtagcact tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg
    13501 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    13561 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    13621 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    13681 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    13741 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    13801 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa
    13861 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    13921 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca
    13981 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    14041 aatttccttt ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca
    14101 cgacgaaaag aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt
    14161 ctaaaactcc gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta
    14221 aacggaagtt cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa
    14281 actgatgtga tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca
    14341 cctttttacc tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta
    14401 aacggacaaa acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga
    14461 aaaccattca actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg
    14521 gcgatgaaat tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag
    14581 aggaaaaaag ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag
    14641 tggtggttca tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct
    14701 ttttatcatt atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg
    14761 gtggttattt tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg
    14821 gcgtacttgc tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca
    14881 tgggatctgt tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg
    14941 gttcatttct ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca
    15001 ggaaaacggg taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca
    15061 aattggaaaa ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc
    15121 tatcactttt ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat
    15181 agcgaacaaa agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg
    15241 attaccgttg ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca
    15301 ttaagagcca tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct
    15361 tatatttgga gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat
    15421 gcatggtctt atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt
    15481 gaaaccatcg cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg
    15541 agacgcttgt tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat
    15601 ccagtcgtag aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa
    15661 atcgatcaac aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc
    15721 ccaacattgg gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg
    15781 aagaaaatcg atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga
    15841 gcttttatcg ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc
    15901 acgttgaatt ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca
    15961 ttaactgatt acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa
    16021 gacggttata tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa
    16081 actcaggaaa atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt
    16141 agtagaaagg agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag
    16201 tgtcggtttc ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg
    16261 ttttccagca actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc
    16321 tattttgctc attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt
    16381 tgcgcttgcc cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt
    16441 tggagactta ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg
    16501 gacaaaatgg gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt
    16561 atatcgtgta tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa
    16621 ttcgctttct gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc
    16681 tttattgatc agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg
    16741 gatagcagtc gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt
    16801 cgcgttgatt gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg
    16861 tgcgccattt attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc
    16921 ggctgtctct gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta
    16981 tcagacgcta ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca
    17041 gcgaggaaac caatttttag gaaaccacaa tagttataat tctattttga atatctatgg
    17101 attgatagga tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt
    17161 gaaagcagat ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat
    17221 gcagtcattt atggaggata cattgacttc ataccgatgg gttccgatta tttatatcct
    17281 gttggctatg gccgttcatc gatatgatga accagaagtt acggaggaat tctatgttat
    17341 ggaagaggca tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag
    17401 cgctcattct tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt
    17461 gacaaccgtc gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga
    17521 aacgttagag aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt
    17581 accaaaaata tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca
    17641 caaatgttta aagatttgta tttatttatg aaggacattt atcagaacag aaaattactg
    17701 ctacagtttt cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta
    17761 tgggcgttta tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga
    17821 ctgaaagcac aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg
    17881 attccctggt tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat
    17941 acttatttag taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta
    18001 tccaatctgt acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat
    18061 ggtatatatc cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct
    18121 ttcttgacag gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg
    18181 caattaatta cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct
    18241 aatcagtttg attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt
    18301 caaggttatc gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact
    18361 ttgtatttct gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga
    18421 ttgaaaccac atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta
    18481 gactaaaaga tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg
    18541 aagccctcaa tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga
    18601 atgtagaaat aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat
    18661 ctacaatgtt gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg
    18721 aaggaaaaat ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct
    18781 atgaaaatat ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa
    18841 tggtcgatga tatcattgaa tttgctgaca tcggcgacca tatttatcaa cctgtaaaaa
    18901 cctattcaag tgggatgttc gttcgtctag cttttgctgt agcgatcaat gtgaatcctg
    18961 atattttgat tgtggatgaa gcattggcag taggagatct tgaatttcaa ctaaaatgta
    19021 tggaaaaatt tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata
    19081 ttagttcgat tcgccgtttt tgtgatcgtt cctattggtt gaaaaatgga aaagttgtcg
    19141 aatatggtga tacgatggaa gtaacgacga attacgaaaa ctttttgaag aaaaaatcag
    19201 ttaaaacagt agatcgaaat aaaaaccaag tagaacgctt cgcagctcca gatatcgtag
    19261 acgtgacgaa agccgaattg ttggataaag ataaaaatcc agtggatatg ctggaacaag
    19321 gagaaaaact atacgttaaa gtaacctatg aagtaaaaga cgatacaatc aaaaatccag
    19381 ttttaggaat tgctctgcgt acagtcgaca atttttatgt gtgcggactc aatactctac
    19441 tagatggtat caagatccct tggaaaaaag gtgagaatat cttttatctg gagtatgaaa
    19501 agttaggttt gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa
    19561 cggttccttt ggtatacaag acaaaataca tgaacttgtt tgtcagcggt tcttatatcg
    19621 gagaaggcat cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat
    19681 gattttgaaa tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa
    19741 ccaggaagta aagttttaga gtttggtcct ggaaacggcc gattaacgaa acatttgatc
    19801 ggagcaaaac aatgtgaagt gagcattgtc gagctagata aggaattgtt cgattttgtc
    19861 agcgagtttg cccaagatgg attttatgga gatatcgaaa gttttgaatg ggcaaactat
    19921 tatgctggac aaacatttga ttacgttttg tttgccgatg ttttagaaca tttggtagat
    19981 cctggaaaaa cgttgaagaa agttagagaa tttttgaatg aagaaggaga aattctaatt
    20041 acattcccta accttgcgca taattctgtc atgattgatt tgttcaataa tcaattgcca
    20101 tgggcttctt atggtttgct agatgaaacc cataattctt tttatactca tgatggcttc
    20161 caaaaagttt ttgaaaaagc aggattatac atcaatattg aagattattt gtacttagct
    20221 gtaggtgata cagaattaaa atcaacttat gaagaattgc cggaagctgt acgttatgat
    20281 ttcaaaatgc gaccatttgg cgaagtctac caatacttct tctcattgat gaaacatcca
    20341 gtagcacaaa gttccattgc gcagccgcaa aattctaatt atgtgaaagt attagaagta
    20401 atccagcaaa cgaaacaaga tgaaataatt caacaaatac cattcaataa tttcacaggg
    20461 gaaaatgaga cactaagttt ccatgtatca gaattagcag aacgaatggt gtttcgcttc
    20521 gcgcagcaac ctagctttat cgaattcagc gcagaagctg ctggagaaaa actcacattt
    20581 atcgattcaa atgctgtagt caaaacagta aatgattgtt atttgtttga tggtaaagat
    20641 ttaccagagt tctttttaac ggatattgca gggaaagaag tgactatcca ttgtcactat
    20701 cgttttatcg gagaattgac accaacaatg aaagaattgc tggaaacaat ccgtccaatg
    20761 gctgaagtta caaagcagct ttctgagaaa aatgatgaat tagaaagaga aaatcattat
    20821 cttattgaga ataatacaag attagatcag acgttgaaaa caaccataaa tcgctattgc
    20881 acgttgttgg aaagtgatga atggacaatc aaacatagat tagggcgacg taagaaagaa
    20941 acatcgaaaa aaatccaaga aaaagagctt tctctatgta ttgacgaaaa aatctgggat
    21001 gcagaaacaa agatattgaa aatcatcggg tggggaatcg ctaattcgga tcgccaacca
    21061 ttgtcctata agttatcagc cgaccaatca ccattttttg aagcaattcc tgttttacga
    21121 gaagaagtca atcaagcaaa acagctagct gaaggagcaa aagcaggatt tgaactgcgg
    21181 atcctttgcg agaaggaacg ttcattctta gttgaagcag tggctcaaaa tggtcaatca
    21241 tggatcattg aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc
    21301 aaaaaaaaga aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt
    21361 tgcttattgt gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag
    21421 aaaaagaaaa tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat
    21481 ataatcttga tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag
    21541 gaaaagcaat cttagacttc caaacagcaa gacatcaaaa tggcatagct gtgaaattaa
    21601 acggtaaata tccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac
    21661 tgaaaaaagg gataaattat gcaaggactc atggcgtaaa aaaagcaatc cgccgggtca
    21721 aactggaatt gaaaccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac
    21781 ctgatctgaa agagcaaagg aaagaagtac aaggatttgc ctaccgccca ttgatttcca
    21841 tacttatgcc tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac
    21901 ttgatcaaac gtatgatcac tgggaactat gtatcagtga tgatgcttct actgatccag
    21961 ctatccgaaa gtgtctagaa tcctatcaag caaaagatga tagaattaaa gtcgttttcc
    22021 gacaagaaaa tggacacatc agcctggcaa ctaattctgc attggaaatg gcagaaggag
    22081 agtttatagc gctcttagat aatgatgacg aattacctcc atttgcctta tatgaagtgg
    22141 caaaagtgct aaacgtacat ccagaactgg atttgatcta cagtgacgaa gataaaatcg
    22201 atgcagatgg gaatcgcttt gacccgcact tcaaagcaga ctggtctccc gatacattga
    22261 tgggaaataa ttatatctct cacttaggcg tatatcggac aagcattgtt aaggaacttg
    22321 gcggtttccg taaaggatat gaaggctctc aagattatga tttggttctt cgtgtgacag
    22381 aacaaattcc tgcagaacac atttaccaca ttgaccgtgt cttgtatcat tggcggacga
    22441 tcccgggatc aactgcaagc aatggagaag ctaaaagcta catctatgat tctggagtga
    22501 aagcattgac agatgctcta tctagaagaa atatcaaggg aagcgtacat cctggcagaa
    22561 tttctggttt ttatgaaatc acttatgatg ttttgcaaga agacttggtc agtgtgatca
    22621 ttccaactaa gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa
    22681 cgtcttatcc aaattatgaa atcattattg cagataatgg aagtacagat cctaaaatgc
    22741 aagaattatt tgcagaatac aagcatcagc tgaaagaccg atttatcgtc gaattaattg
    22801 acattccatt caattattca cgtatcaaca atctggcagc agaaaaagca aacggcaaat
    22861 actttttatt cttgaataac gatacagaag tgattgaacc tgactggatg acagcgatgg
    22921 tttcttatgc acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg
    22981 atacaacgca gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga
    23041 ataactatga tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact
    23101 tagcagtcac tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt
    23161 ttgatgaaac attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac
    23221 tgggtcgtta caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa
    23281 gaggttatga agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc
    23341 aagataaatg gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag
    23401 aaggaatcgg cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg
    23461 tgattcgctc gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc
    23521 gactcaaaaa aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa
    23581 attcacgaaa attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct
    23641 tctgctccca ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc
    23701 ccacgctctt attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg
    23761 aaccctagct gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg
    23821 tttaaggtat tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt
    23881 gatggagata accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat
    23941 tgctgaatat atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt
    24001 ttgtaagtct tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg
    24061 tacgatttgt tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag
    24121 atcacttgcc gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa
    24181 ccagacattg gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc
    24241 agcataataa ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa
    24301 atcaacagtt gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac
    24361 agtttgatag ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt
    24421 ttttgtccaa tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg
    24481 tgctttcatt gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt
    24541 cactttatac tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg
    24601 ctctaaagca atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca
    24661 agtatcgtct tttttataac cattccattc atctgtataa acttctaccc catccttata
    24721 tgcgacaatt tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt
    24781 attcataaga tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca
    24841 attttttcga ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag
    24901 atggattctc tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata
    24961 cttcgacttc tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt
    25021 tagagtcctt gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca
    25081 ttacaatttg cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt
    25141 attttgtgat taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga
    25201 tattcattat attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac
    25261 tgaagtttcg tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta
    25321 ttatttcagt gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc
    25381 gaatgatcag tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga
    25441 tcatggtagt gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt
    25501 catttgttct cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt
    25561 tacgcgtaag tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag
    25621 caatccaaaa ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg
    25681 ataactatta tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt
    25741 ctcatatcga agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa
    25801 aattgttttt gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt
    25861 tcgaagatga aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat
    25921 tcaaacgttt gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta
    25981 tgttattcac agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag
    26041 tgcggatcac aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca
    26101 cagccgaagc aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg
    26161 taggtaaatt tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga
    26221 aaggcgatat gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca
    26281 atgcagaaaa cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg
    26341 cacaagtcta tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt
    26401 atatcaaaaa atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc
    26461 tatttgataa aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag
    26521 aaattgcttt tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc
    26581 cgaaattcag cataatcgta ccagtataca atgcagaagc aactattggt cgaaagattg
    26641 atattctaaa agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa
    26701 cagatcaaac acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga
    26761 ttactacgac caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag
    26821 gagaatatct cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt
    26881 atgatcattt gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc
    26941 gtacaatgga cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact
    27001 atctaactca cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt
    27061 atgttgtatg gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta
    27121 aaacttataa tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc
    27181 aagtgatcat gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca
    27241 ctaatcaata ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata
    27301 ctgtaacaga cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt
    27361 cagttatttt ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag
    27421 caaagcagat attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt
    27481 cttcagcaaa aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg
    27541 ctgtaaaagt tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc
    27601 gagtatatta gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa
    27661 tgggtttagg tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa
    27721 actatcaatt tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag
    27781 agattgaaag attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta
    27841 aatatttaat agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc
    27901 aggtttattt tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga
    27961 gaattgctca tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat
    28021 tgcgtaaatt tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc
    28081 aagaagctgg tgaaagtctg tttcaaaacc atccctttga gatagttcat aatggtattg
    28141 atttagagct ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata
    28201 tctcaatgag tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat
    28261 cttatttgtt agagattttt ctagaaatac taaaaatgaa ttctgattct tacttgattt
    28321 tagtaggtga agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag
    28381 agaaaagagt aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg
    28441 atgtttttgt aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag
    28501 cagcaaaatt aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata
    28561 atgtggattt tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag
    28621 agccgtatgc taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg
    28681 aacaaatgga gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg
    28741 attatttttg aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag
    28801 attggtcaat cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga
    28861 tatagaacca tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa
    28921 aaaccgtgtt aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt
    28981 aaagagagca ttacaaaatg agaaatattt aaaatatgta ttattatctg gtg
//
LOCUS       DACDCJ010000031        28259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDCJ010000031 DACDCJ010000000
VERSION     DACDCJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.031"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            142..1227
                     /locus_tag="IV038_001338"
     CDS             142..1227
                     /locus_tag="IV038_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8341567.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAKIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKYKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            2038..2370
                     /locus_tag="IV038_001339"
     CDS             2038..2370
                     /locus_tag="IV038_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8341568.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKAVVQCKKYI
                     IQKDKVSEEMIYYTGKRKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            2961..3290
                     /locus_tag="IV038_001340"
     CDS             2961..3290
                     /locus_tag="IV038_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341569.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            3277..3618
                     /locus_tag="IV038_001341"
     CDS             3277..3618
                     /locus_tag="IV038_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8341570.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3732..3971
                     /locus_tag="IV038_001342"
     CDS             3732..3971
                     /locus_tag="IV038_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341571.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            4038..4277
                     /locus_tag="IV038_001343"
     CDS             4038..4277
                     /locus_tag="IV038_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341572.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            4363..5034
                     /locus_tag="IV038_001344"
     CDS             4363..5034
                     /locus_tag="IV038_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8341573.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5087..7063
                     /locus_tag="IV038_001345"
     CDS             5087..7063
                     /locus_tag="IV038_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8341574.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            7076..7831
                     /locus_tag="IV038_001346"
     CDS             7076..7831
                     /locus_tag="IV038_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8341575.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            7828..8652
                     /locus_tag="IV038_001347"
     CDS             7828..8652
                     /locus_tag="IV038_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341576.1"
                     /translation="MNKRKWLFVCVAALFSTAAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQPVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            8942..11053
                     /locus_tag="IV038_001348"
     CDS             8942..11053
                     /locus_tag="IV038_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8341577.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDNKNFDMVSDGEKYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(11091..13148)
                     /locus_tag="IV038_001349"
     CDS             complement(11091..13148)
                     /locus_tag="IV038_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP8341578.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(13262..13474)
                     /locus_tag="IV038_001350"
     CDS             complement(13262..13474)
                     /locus_tag="IV038_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341579.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            13661..14128
                     /locus_tag="IV038_001351"
     CDS             13661..14128
                     /locus_tag="IV038_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341580.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            14125..14367
                     /locus_tag="IV038_001352"
     CDS             14125..14367
                     /locus_tag="IV038_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341581.1"
                     /translation="MIASLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14385..14687
                     /locus_tag="IV038_001353"
     CDS             14385..14687
                     /locus_tag="IV038_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341582.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            14759..16813
                     /locus_tag="IV038_001354"
     CDS             14759..16813
                     /locus_tag="IV038_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341583.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            16813..19425
                     /locus_tag="IV038_001355"
     CDS             16813..19425
                     /locus_tag="IV038_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8341584.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            19422..21953
                     /locus_tag="IV038_001356"
     CDS             19422..21953
                     /locus_tag="IV038_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341585.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKIFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLSKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            22003..22335
                     /locus_tag="IV038_001357"
     CDS             22003..22335
                     /locus_tag="IV038_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341586.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            22336..22929
                     /locus_tag="IV038_001358"
     CDS             22336..22929
                     /locus_tag="IV038_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341587.1"
                     /translation="MRVKGQNLVDKKSIFQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            23092..25116
                     /locus_tag="IV038_001359"
     CDS             23092..25116
                     /locus_tag="IV038_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8341588.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            25116..26321
                     /locus_tag="IV038_001360"
     CDS             25116..26321
                     /locus_tag="IV038_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8341589.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            26337..26981
                     /locus_tag="IV038_001361"
     CDS             26337..26981
                     /locus_tag="IV038_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341590.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26992..27798
                     /locus_tag="IV038_001362"
     CDS             26992..27798
                     /locus_tag="IV038_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341591.1"
                     /translation="MSKKKGARQRFKKRKSYLQCLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYKQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTKNKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            27820..28050
                     /locus_tag="IV038_001363"
     CDS             27820..28050
                     /locus_tag="IV038_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341592.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYIAAKKYFTAERK"
BASE COUNT        10221 a         4357 c         5678 g         8003 t
ORIGIN      
        1 agtataactt atacttattt ttttaatata taacttatac cttaaatgct gaaagtgttt
       61 tattcaccct taatacatgg tatagttcac ttgttgaaaa tcgtgaatat atacaagttt
      121 ctaagaagaa ggagaaattt aatgtctaca gaaaatcaat ctcaaaaaat gattattaaa
      181 aattggggat ataaagcaac gaatgtttat attgaaaaat attataaatt tcctagtttt
      241 ttaattcaga attctaaata ctctaaaatg aaagacagct caaaaatagc ctatatgctt
      301 tttaaaaatg aatttcgtag agccttacat gaaaaatgga tcgacgatga tggtctatta
      361 tatttggaat ttacacaagc agacctaatg gacaaattaa attgctacca aggcaaagta
      421 aaaaccattt taaacgagtt agaaacatat aatttgattt cagtcgtaaa aggagaattt
      481 aacccaaaaa cgaaaaaaaa tgaaaaaaat aaatactatt tacttcaacc tgaaataagt
      541 gccgatgatc tttttattca aaattctttc gatgacgatt taagcgaaac tattgataaa
      601 tcaacctctt ctcggaatgc ggaaatcgca cgtcgagagg aaactgctaa aacccttgat
      661 aatactggaa atgcggaaat cgcacgtcga gaaaaaacag ctgaaaccct tgataacact
      721 ggaaatgcga aaatcgcaca agattattat attaataaat ctttagacac taagagacac
      781 ttaaaagaca ctgaaacaga ccaattacaa aatcaagttt tgctagataa cttcgttgaa
      841 atcatgaaag aagacagcat aaacacattt gtaccagaaa aggtattaag cttgataaaa
      901 acattttcta acacttacga agaagcccaa caaaccgtca ccacaataca caacgcaaaa
      961 tataaagccg aggaacttga gaagacggta gttgtgtatg aagaacttga atcttacgga
     1021 attgatgcgg ataaaggatt atatatgacg ttgctgaaag cttatcaaaa acaaaagact
     1081 gagaaagtaa ataatttgca gaatttgata tttgtgtatg taaaaaactg gtttattgaa
     1141 aaagctattg cggcaaagct tgcaaacgaa caaagagaaa catttaacga attaccaaca
     1201 gttagtacgg aaaattggct agagtagttg atcaatttta aattagaact tttttataat
     1261 tttaatttta ttacaaatgt tacaaataaa tagttattat tcaaaggttt taagattaaa
     1321 agtttatgaa aacaatcaca aacgtaggtt ttaaattcat aatcgttaga tattttagtt
     1381 atttttcatt caaaaaaata gggaactagt gtttgcatat gtgctttttt gagttataat
     1441 aattcatgaa gagaggtaat tcacttattt ccccaagctg aaaagcctga tattacttct
     1501 tttcaggcag tgctgctaat attcaattac acttttttgt atatttttag ttgttttaaa
     1561 tttaattgtc tgctatgctt gtgatgaatt gattttcgtt ctgcacttct ggacgataaa
     1621 ttgtgataat tgaaaataca aaaaaaagaa aggcccctgt aaggctcggg agactggtaa
     1681 tctccgtcag tgtcctacag gaaagcatta agatgacgcc ttaatgcgtg cccttcagtg
     1741 ttacggtatt acccgcaacg gactcagtat aacagatatt caaaagtgat accacctttt
     1801 ttggctctat taaggttaaa aatacgtgat attgtaatga atctatgcct aagcataatg
     1861 ttataaaaat gtccgtgtgg ctctttgccg tatgacgaat gctgaaatta gagggaacgc
     1921 atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt
     1981 gaaaaataac aagaaaaact tattaaaaat atttaaagaa agtgagagga ttattttatg
     2041 tcatatacag gaattttgag tctcgaagat atttgtcatt acggtaaacg gtgtaccgct
     2101 acagagaaaa tcacgaagaa actatcaact gggcaaagca aagctgttgt gcagtgtaag
     2161 aaatatataa tccagaaaga caaagtttca gaagaaatga tttattacac tgggaaacgc
     2221 aagcaaataa ttttaaaaga tcctatccct ttaaaagagc tatatccaac aatcaaacat
     2281 gtttatgacc aaaacggtgt actgattggt cgaagaaaaa atggagtctt gcgttgtacc
     2341 gcaaaaggga tggggcgttt aatcagctga attaatcact ctcataactt gtattttatc
     2401 gtttttcgct tgcttgaaaa tattgtcgag acttctaaag gaacttagaa tcattagaaa
     2461 aataaggagt agacttgatc tatcaacaat tcctttcggg gggtgacggg gtaggttttt
     2521 acgattggta acgtaaaaaa ttggtcaatc gagtaaatgc ccttgtttgg atagtggtga
     2581 aatgaatttg tgtaaataat atgcacagag taaacaagag ggaatttaga agttagacaa
     2641 gttagaatag cgcctagatt aatcatgagc agctactaag ggatctaggc tttgacgcaa
     2701 actcatggaa taaggagtag gtcttctcgt aatggggagg acgcacaaga aattgtgtcg
     2761 tgtgctgcag tacatgtaga tacttgcgaa gacgttgatc tttggatcaa tcgagcattt
     2821 accgttttct actagggttt ctcgtttcgg acgccataaa cgagtcgatt tattttatat
     2881 atttttgagt gaacgaaaaa cgataaagat attcgttatg aagtaaaact aatttagtag
     2941 atagcaaagg tggtagagga atgattcaaa aatttgcgaa aatgagtaaa actgcatgga
     3001 ttgaaaagct tgaaaatgac ttgagtgtca tgcgattagc tccaaagaaa ttcaaacgag
     3061 agattgcaga aaaagaagca attttgtcgg tattgaatgc ggtagacgat aaatgtccag
     3121 taaaaattag tttttcagat atagcaacaa ttatcgaagg tatttattat ggtgaagttg
     3181 atgaagagat atcttctaca aaagatttat tagatttttt ggataaaaac aaagatacct
     3241 ttatagtact aactgattat gaggaggtta caacaaatga aaaattatga tccgaatata
     3301 cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa aggatttgtg
     3361 acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga cgaggatgat
     3421 ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc tgaagatgat
     3481 gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga attatctgtt
     3541 gaagatactt ggtcatattt gagtgattat attgtcagcg ttgaaatcat tgaatttgta
     3601 gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc ttagaaggag
     3661 tcacgacgat taataaaaag gtagtattac cttaggcatt gagtaaggaa tttatttagg
     3721 aggaaaatta gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag
     3781 gaaggtatgt acagctgtta gctcgtggtt attcacctaa agatgtattc aaatccctgt
     3841 ctaaaggaac agatatggaa aaagaaagat tacgaaaaga attcgattat tggagaaccc
     3901 ataatggaat caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa
     3961 cccaagactg aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa
     4021 ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag
     4081 aaatggaaga aaagttgcta aaaacgatga aacaaaaaca ccttaaaaga ttatctgtca
     4141 tgcaatatat caatgatatg cagataacag gaaaagagaa agcttgtttg ttaggttcaa
     4201 tgaaaaactt tgaacagttg agacgaactt atgtgaaaaa aagttctaat tgtcaactat
     4261 tgttagaagt ctcatgacat tcattaatag aattcatttt taaaactttt tctatggaaa
     4321 tgagaaaaag attttttgtt ttgtctgaaa gggggtagct ggatgagaaa atggttattt
     4381 ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt ttggctatgt tagctaccaa
     4441 aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa agatgcccgt agatcaaatc
     4501 aatgcacacg caaagagtta taaggaggat gcaacgaacg tcaataatga tatgtctttg
     4561 gggcaaatgt tgtccataca aaaagaagca atcgaaatgg gcgtcaataa acaagtattt
     4621 gcacaaattc agattcctgc acttggttta gcattgccaa tttttaaagg ggcaaatcaa
     4681 tacactttga gtcttggagc ggctacttat ttttatgaag atgctgaaat gggcaaagga
     4741 aactatgtcc tagcaggtca taacatggaa atgccaggtg ttttattttc tgatattcaa
     4801 aagttgtcat taggggaggt gatggactta gttagtaacg atggcgtcta tcgttacaag
     4861 gtaacgcgta aatttatcgt acctgggtac tttaaattaa tcgatggcgt tcctgaagag
     4921 aactctttct tgtctttgcc aaagaaagga gagaaaccct tattaacgtt gtttacttgt
     4981 gtttatacgt cacaaggaaa agaaagatac gtggtacagg gagagctaca ataacaaggt
     5041 tttaatcaac gcgagtcgtt gaaatatgaa atgaggagag taaaaaatgg caataaatcg
     5101 gtatgtaaaa aaagctggaa tgcttgttgg aatgttagga atcattgcag gctgctttca
     5161 ggggtatcaa gtacatgcag aagatgtcac gcaaaaaaca ccaccagaga aggtcaatat
     5221 cacggttcat aaattaatgt acgatcaagg aacgcaattg aatgttgata ttgacgggat
     5281 caaaaacgat ggttatacgc acgatcaata tcctacaggt gtaacgaaat acaacaaggc
     5341 agattatggt gatgttgagt ttacgttagg aaatattacg gatcaagtat taccgagaga
     5401 agattctgat ctaaccaacg cgaaagtgga cgagattgta caagatgtag aagacaaggg
     5461 atctaacagt gagtacgtca agaatgcaac caacaaaatt acttcagctg tagatgaaaa
     5521 tggtgagatc acttttactg atcaaccagc ctatgtcaat tccaaaggga atgtatatgt
     5581 ggtttatgaa agtaaatccg ctgctggatt agtcacacaa aaagcaaaac caatggttgt
     5641 gattgcgcca atgacagata aaacaggatc aggattttta aaaaatattc atttgtatcc
     5701 taagaacatt gtttctaagt tgagttttga attaacgaaa tttggtgacg atggcgcaga
     5761 ccaaagcaaa caaacccttt taaaaggggc aaaatttgag ttatacaagg gcgaacctgg
     5821 aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa ggaaagctga ctgcgactga
     5881 tttgacgtta ggaaaatact attttgtgga agttccttcg gaagttgtag tgggatcaga
     5941 tcaagaacca acagcagatc aatatttatt aggcgcagac gctcgcaacg atgcgcacaa
     6001 taaattaacc tttgaaatta cgaatgatgg cgtgacaagt gacttaaaag cttcatacgt
     6061 aaactataaa gcaccagtga tcgataaaac agtaacaaat ggaacaggac aagaacattc
     6121 tttccaaatt ggtgacgcag taaattatca aggaacgatt catattccaa cagacattgc
     6181 aggtggcgca gacggtatca cagtaaacgg tgtgaaaagc gaaacttctc cttacagtgt
     6241 atttaaatgg ggagatacag caggacaagg actttcttat gtcgctgcta aagcaaatat
     6301 caaagtaaca aataaagatg gatctgtggt attaaaagaa aatacagatt ataaaatcca
     6361 aaattcagaa aatggctttg tgatcgattt tatcgtgaat aatggccaag taagtgatac
     6421 tgtagcaagt ttacacggac aagatcttca aatgacttat aacatgtatg taaatgacag
     6481 cgcagctgtt gccaatccat taacaaatag cgtggatttc gtttatagca acaatccatt
     6541 caatcaggaa gaacaccatg aaaaaacgaa agccgacgct gtgacttatg gcgcaaaatt
     6601 cttaaaagct gatggcggac tatttggaac aggaatcaaa gcaacaccgc ttgaaggcgc
     6661 agaatttgct gttaaaaacg cagaaggaaa atattatggt ggattagttg atacggataa
     6721 agacggtgtc aaagaagcag tatgggtaga cgatgtagca aatgcagcga tcctaaaatc
     6781 agataaagaa ggtcactttg aaatcactgg tttaactgaa ggtgaatatt cactggaaga
     6841 aacaaaagca ccaactggtt accaaaaatt aacagaagat attgcattta aagtaaacaa
     6901 aaactcattt aaagaggaaa atcgaatcac tgttaaaaat aatcaaaaag cagctattcc
     6961 attaacaggt agtaatggtt tccaaacgta tgtattgatc tcttgcttgt tgctaggagc
     7021 aggggcactt tctgcagttg tttactttaa gaagaaagcc tagaaggatc agctaatgat
     7081 tattcgtaag cccccaaaaa gaagagtcat gcacaaactg atgatatttt tttggttgct
     7141 gtcggcagta gcagtctatt taattccatc tgtggaaaca aatgtagcga aatttcaaca
     7201 acagcaaaga attttagcca ttgaaaatct tgaaaaaaca gattcttctt tggatgttgc
     7261 gaaaatagag ttaggcgatc ctgttggtgt tcttacaatt ccaagtattt ctttaaaact
     7321 accaatttat gacggaactt ccgacaaaat tttagagaat ggtgtaggga ttacggaagg
     7381 aactggcgat attacaggag gaaacggtaa aaatccttta atcgcggggc acagtggact
     7441 ttataaagat aatcttttcg atgatttgcc ttcagtaaaa aaagggcaaa aattctttat
     7501 taaggtaaat gaagaacaac atgcctatca aatttatcga attgaagaag ttaaagcaga
     7561 agaattacaa aaaaattatt tagattatct catgcctgaa aaagaccaag ataggatcac
     7621 gttaatgaca tgcacaccaa aaggtgtcaa ctcacaccgc tttttagtgt atggtgaacg
     7681 agtttccttc agtaaagaag atttaaaact acagaaaaaa gcaaaaaata ggaaatcaat
     7741 ttcattggat tggcgattag cactgctatt cgcttttttt tgcgttccta ttatttttat
     7801 ttataaaaaa ataaggagga gatcggaatg aataagagaa aatggctgtt tgtttgtgta
     7861 gcagctctct tttctactgc agcttattcg caactgtctt tcgctgacag cagtgatagt
     7921 agtaccactc aaacaataga taattcaatt actgcaccaa gcgaaacgga cagttcggag
     7981 aatactacag aagaaacggc agattcaaat ataagcttta ctgaatcaac gaatccaacg
     8041 tcagaaagtt cttcagagac ttcagaaaag ccgcaagacg tagaagatac gcaagtgatc
     8101 aagcaagcca ttcatattca aaaaattatt tcttcgaatc aaatcaacaa tcaaggcagt
     8161 caagtagccc agcaagaagg tatcaatggc gcaaagttta ttgtctacga tatcacgaat
     8221 atcttagaag aaatgtcaca agatgataat gagaaaaatc aacctgtaga aataattgag
     8281 tcaaaattga aagatcgagc aaaaaaactt tcatacgacc agttgaaaaa agtggctgaa
     8341 ggagaaacca aaacgattga tgatcaggaa ggcgtgattg aattttccat tgaagtacct
     8401 gcaaacaaaa aacaagcata ctatatcgtt aatgaatcaa gccctgaaaa catttcaaac
     8461 agtgaagata ttgttttatt gacacctgtt tcaaatgaaa aaggtgaatt tttgacggat
     8521 gtttggttat accctaaatc agagaaatca gagccgaaag aagaaattaa aaaagttgtg
     8581 tcaactggtg taaagaagaa cttttttgaa aattgttggg aattttttgc tcaattattt
     8641 tttgataatt aggatgaaat gatgaaaata ataagaatat tagtttttaa tctatccttt
     8701 ttagccgcaa cattattgtg ttgcggttta attttttctc aattatcaat ttatccaaga
     8761 tatcaacgat tgttcattca ttttctaaac attcaatgtg cttccatgcc attagaggtg
     8821 attaaaaagc ttttgatcag tccaattttt atggttagtt tggttcttct tacaggattg
     8881 gtaacgtggg tattaggaaa aaaagaatga aaagcttacg atcgtttaaa aaaaatatta
     8941 gatggagggc attatggaaa ataaaattca ttgcactacg ggaagagtaa ttactttagt
     9001 gttgtttttg ttccttcttc tttttgcgag gggaactgca gtccaggcct tagaaaaagt
     9061 tgatccgagt caaatcaaag aaaataataa tctggtgctt tatcatgata gtgattcatt
     9121 gagaaatatc gaagtaactg ggatcaatgg atttaagata ctagatgagt attcaactaa
     9181 aaaacaaaaa gtacttgctt ggccaaaagt caaagatttg aaaagtacgt caaaaatcgt
     9241 tgcccattat caaaaagtag gtacttatct aggaaaagaa attgattgtg acgtgacttt
     9301 ttctaatttt gtagcgcacg ctggggttaa ccaagacgga ttagctaaaa caattaatgg
     9361 caaaaagtat tataattatt tgctctttga taaaaatatt tatcgaggat ttttcatgca
     9421 gtcaatcaat gtcatgaatg caaaactaga atttaagtat gcaggaacaa aaacaaaagt
     9481 agctttgagt gaaccacagc cagaaattca atcaaaaatg agatcgatga gagcagctgg
     9541 tattgattta aacgatacat ttctatccat ttatagttta aatggacagt atcagcaacg
     9601 tgtgtcttgg tataatgaca ataatgaatc tgtcggtgaa cgtaatattg atatgagaga
     9661 atttgttggg tatgaaaaaa tgggtagctt accttatttt gtcacaacag atacagcatg
     9721 tgcagaatac aaagctcctg cgttatcaac aaacaattta acttcaaaag tagtgggagg
     9781 acgcgcagaa aaggcttata gctcgaatga tcatttcacc gatgttgtag gagctgatac
     9841 ttatcacaga agtggtgtaa cgtatacgct tcaaggcgct tccccaacat tcatgattgg
     9901 cgcaaatacg aatagtatga tgtttagctt tgatactgca ttgctatgga caccacaacc
     9961 atcgaagcct acaaaagaag tgtttaacaa agctaatact gaagaggcag cacacaatat
    10021 tgacaaaaaa gtgattccac aaggatcaga tgtttactat catattcacc aaaagtttga
    10081 tgcattaaca gtcaacacaa tgaacaaata caaatcattt aaaatcactg atacctttga
    10141 caacaaaaat tttgatatgg tatcggatgg ggaaaaatat gatggcgcat tgtggtatgt
    10201 tgaagcgaat ggtacttgga aaaaagctga tacaggagga aaattaactg tatcaggtaa
    10261 tacagtcact tatactgctt ctgacagctt tttagctaac gctgcaaatt acggtaggga
    10321 ttatattttg cagctccata tgagagcaaa aaatgatgca aaaggaattg caccaaatgt
    10381 ggctactgta acgtttaaca atgatccgaa caaaggaaca caaaaaacaa atacagtaga
    10441 aaactatttc tctcttaaac caaataaaaa agtggaacaa gatgggaaag atgttgatgg
    10501 tcgaaatgat ggtaaaaaag gaacgcctac agctccttta aatgcaggat ctgaagtaca
    10561 atatctagtc acacaaaaat ggcatacaaa aggtgtagac attgcctcag atcattacaa
    10621 gcaatttagt attcaagatc cagtggaaga tcgtttgacg tataaagaag gttctgcgcg
    10681 agtgatagat atgagtacag gaaaagatat tacttcagaa ggtacactaa cctatgatag
    10741 caattcaaga acgctgaagt gggaagcttc cgctgatttc ttaagtaaaa atcttttaga
    10801 tggacgagaa attcaactga tatttacagc taaaactcct ttgaatgaag aattggatat
    10861 cgacaaccaa gcaaaagttt cagtagaaag tattgagaaa gcaactaatg tagtaacgat
    10921 tggggtaaaa cctaacttac ctcagatcat tgtgccaaaa actggatcga atcatttagt
    10981 gctaactgtt attggaagta tattatttct tactttagca tcattgggct atgtagtttt
    11041 aaaactcaaa taaatataaa aacaaggctg agaatcagcc ttgttttctt ttatttgaag
    11101 attggattcg cactaataaa tacattatct acgccaattc taccagcaga gtcaactaca
    11161 aaattatgac ctaatccgag aatactttgg ttattataaa gtaaatttgc attatgggca
    11221 gggctatttt tccatcctac catggcatcg atagcaccat cgtctgataa aaggtcatca
    11281 atatacagcc cagtttgact tatgttttct cccttagcac cgtagcagcc taagctttcc
    11341 aataaatctt ggaaagtacc accaccaggg cgatagtgac tgaagctgac ttgtaattct
    11401 ttagctcgaa tatctgcagc ctgttgaaca atatcattga attgcattgg ggcaacacca
    11461 ttttgttggc gataatcatt taaccaagca agttcttttt gacgcataaa gttacgtaat
    11521 tcgtctaatg aaatcttttt atatttgaat gtgattgttt tattttgatc gacgttatta
    11581 atggatcgtt ggccgttttt atccacggta acggtaggat cgtcatttct gcacaagaaa
    11641 taattttgat cagtcatgtt tttccaagga gtagcaaagc tatccccttc gtatactgtt
    11701 tttgattcac tagagagaac agcgttggta tcagcattta catgattaac agtcacatta
    11761 aatttacgag tgtagttgaa aatataattt tgactttcag tcacattgtt taaggttact
    11821 gaatttgttg gttctacccg atcgtctaaa acatatcctt gaatagaagg agcagttaaa
    11881 gtgacactgt caccgtgagt gacattgact atctgttctt tcaaaatatt aactcgtcct
    11941 tcgtttttgt agttgttatt gcacaacaaa ttgatttgat gcacttgatt ataattgaat
    12001 gtgattgttg tattacctgt gattgttttg ctttgagtat cattaccaat cactttgtag
    12061 ctaaaattat ctttagcgcc ttcgctatat ttaaatgctt ttgccttagc agtgtaagta
    12121 gaaccagaag ttacttttgc ttgatctgta gcaatcactt gacctgtttg attgtcaata
    12181 tggttaatag taaccgtata ttcttttggt gtttcaggtt tgtgttgcaa ccctttaaat
    12241 gcagcatcaa gactattttt agcattattt acttgggctt gagaagcctt agaatcgtct
    12301 agtacttttt tagcattgga taaagcagtc tgaaaattat tccaagattc ttctgtaaaa
    12361 tctcctttag cagttgattt tactcggttg tataaagctt gtaattcagc tttattcaca
    12421 gtaggaactg gttttttctt taatcctttg taagcagaat ctaatttatt tttggcacta
    12481 tccacttgtt tttgagtcgc ttttgagtca tttaacactt tcttagcatt gtttaaagcg
    12541 gtttggaatg cgttccaggt attgtcggta tagtcacctt ttttagttcc cttaacgctc
    12601 gtataaagag cttctaaagc tgatttattc actttgggtt tagaggtgtc agcgccatac
    12661 caaccgatac cttcgtattt ccagccatag tctttggtta accattgtac ttctttccaa
    12721 cttgcagtgt agtgatgtga gccagatttt acaccaggaa ggaaaaggcg gtagatcggt
    12781 ttattatttt tttcggcaga acgccaggca ggcccttcat aagtccagcc gtaattttta
    12841 gtcaaccatt tcacttcatc ccaactagct gtgtaatggt gatcacgaag acctttattg
    12901 tacaaacgat agacaagatg gccaccgcta gtcggtgctt cccaggcggg gccttcataa
    12961 tttccccatt ttatctttac tagatggtca cgttcttttt tgctagaggt gtaaaaatgt
    13021 tcatgattat ttggattgta aaggcgatac atgacgttcg ttgaagcagc gtcaacagat
    13081 ccagcaagac taagtgaggc agcaaatgtt ccaatagcta aaacagtttt tggattaaat
    13141 aatttcattt tttcaactcc taaatttaat taaaaatgtt tacatgatta ttatatatcg
    13201 gctaaccgat atagtcaata aaaaataaga aaagccttat ataattttgg cttttcttat
    13261 tttatttgtt agttgaagta ttaatatcaa cgatttcttc agccaattta tttaatttca
    13321 tagcatttat gaatctcatg ttgtctatac tagagcgttt gttggcaagg tcacttactg
    13381 tagttaaggg tacgccagat tctatggata tagaacgaac tgatttacca ctatcaaaca
    13441 gcaaccattc gattttcttt ttatcagcgt acatttcaat gctccttctt gttttttctt
    13501 tattatagct cttttttgta ataaatcaca atatcaagag aaaaattttc tatagaaagg
    13561 tggattttta tgagtattcg ttctccgtgt atccaaatga ggtttgttgc tactttcgtt
    13621 atttgatata ctaaaaataa acaacgaaag aaggaataaa atgaagaaaa agcaactatg
    13681 gtttgtctct atgtttgcaa tattttttat tattttaagt ggatgttcaa gtaataagaa
    13741 atcggaagct aaagaagata atagtttatt gcaaccagga actgtttgga aagaagaagt
    13801 aggtggtctt attcataatt taaaaattat agatgatact acctgggaat attctgaaag
    13861 tgtatggcat ccagatcctg tacaaataac agttaagaga caaaaagact atgaagggtt
    13921 agaaagatac aaaatagtag attctgcagg tatcagaaaa tttataaaca aacccgattc
    13981 tttatttatc gttgttcctt acgagaaaga tggcgtaaaa aaaattgttt ttcttggatc
    14041 tagtaaggat gaaaagcgaa cgaaagaaaa attgattcat gatggatcac aatcaaataa
    14101 atacaaatta caaaagacta gcgagtgatt gctagtcttt ttatttaccc taaaggagga
    14161 atcatagtgg caaaagcgaa aacgccattt gaaaaagccg cgtctaaaat cttagaacgt
    14221 cgcggtgtat ctgaaacaca gtggaaacaa gaagtccttg aaaaagcaca atttgaccta
    14281 ttacggggaa aagacaagga cttagaagga tatatctatc aacgtgaacg tgaaaagctt
    14341 gttttcgatg aaattattaa acactaaaag gagcgaacaa ttttatgttt gaatcaatta
    14401 caatgaattt tttaacacaa ccgaaagtag tagctgcggt tgatattttt agagcattac
    14461 aaactgccgc aactagtttg aaaaataatc tgatgatttt tgctgctgtt gcggcagtgg
    14521 cttccattgc tcttggaggt attttcttct tctttgggcg aagaggagct gaaaacggga
    14581 agacacacgt aggttatgta ttgttggggg taggagttat tttagctgcc gcttctattg
    14641 tagtttcaat gttctcaatg tttggacaaa ccccacaagg actataaaaa aaacaagtag
    14701 aaggagtaaa aagtacgtgg ctacttttta ctcctttttt tatgaaagga gaatattcat
    14761 gttttttacg atttcaggaa atgcaaaatt ggataaagag ttaataagaa aagcaacgaa
    14821 gaaagtggga aagcgtactt cctttttacc gaaaacaatt aaatattctg tcattgaaac
    14881 ggaagaacag ctacatgtgt taggacaagt gttagaacaa gagaatcaat cttctgttaa
    14941 attgactctc acgttacatg ataaagaagg aaaaatagaa aaggctggca cagaatacgt
    15001 ttttggccca ctggattatc aaatttcaga aggtttttat cctacattga tggatttaat
    15061 ccgccttgat gtgtttaaag atacggatga aactatgagt gtggaagagt cttatcgaaa
    15121 cgaaaaactt ttacaagttt tagaagaaga gttagagagc gaccaaagaa aagtgaatca
    15181 agtgagtgaa gtcgaagtaa atgaagaagt accatgggaa cgaccaatat ttccaaagaa
    15241 ggagtcaaaa gaaaaagaga cagtctatgt tgacgaacaa gctgatattc cgactcagaa
    15301 atcgatattt gaggaagaga acgatgtgat gggtcaattg gaggtagatc cctttgaaga
    15361 aacaatggag gatgaagaga cggacgaagt agaagttgat ccagttgaag ctaatgaaga
    15421 gtcaacatgg actgaagggg attctcagga atatattgaa ccacttatca gtgaagaagt
    15481 agaaaaaaac gaaacagaga tacaaaaggc aactgtattt gattatattt tggatcaata
    15541 tccgccagag catgaatgga tgaaagaaaa aaacaaatta tttttaaatg agctttattc
    15601 gaaatatcaa ttaccccaaa cctatcaagc atttttagag agtaaagaag aattaatctc
    15661 acagggtaaa ggcacacttt tagaacagtt agagtctgtc caatcaacaa attggcatga
    15721 gcttgcacaa gaagatttat tagccacctt tgaagaacgt caacaagaaa cagatcaaga
    15781 aattaatgag tatgcgacgg aacagtcgtc taattgggag aaagtaaaag aggaactaaa
    15841 taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa atcaaagagc ggtacgaaaa
    15901 gaaacgcgac aatgcttatt ccttgtgtgt agaaaagatc aacaatttta ctaagacgaa
    15961 tcaagaagcg ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtatta aagaacgaaa
    16021 agaagagttc taccgcggtt tacgtacaga taagctaaag attagcaaag aattaaatga
    16081 acaactgaat aagctatatc aaacaacgac tgaagttctt atggaaaaac acgaacagat
    16141 ccagcaagaa ttacaagtac aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca
    16201 agaattgaaa gaacaagaag aaaaacaacg gcaaaaagaa aaagagcgtg aacaattaga
    16261 aataagaaaa cgggaacagg atcttgcgga acaaaaaatg gcgatagaaa aacaacggtt
    16321 agagcaagaa aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac
    16381 attgcgtcaa gcacagttga attatctagc acaacctcaa ccagtagctg ctgttcaaac
    16441 gccagtgaac aaccaagcaa atttaactcc ttcaccccag cgttccttta acggatggat
    16501 ggtgggatgt attgcaagtt taagcttgct tctaggtgga ggaacagtat ttgcttttaa
    16561 tcatatcaat caatcttcac aggctgattc tgtagccaca acggcgtcaa tcaaggaaga
    16621 agcccaggaa caagcaagaa aagagtatga acaacaatta gaagaattat tgaatcgtac
    16681 caatcagtca acaacagagt caagcatgga aaatagcaaa gagagtgatt ctagtaaggc
    16741 taaagaagga tcaagcgatt caactgtaca atcaaacact tccgaaagtt cagaatcaaa
    16801 tgaggtgaaa taatgcgtgt tagagaaaca acgaagcgaa aagacaaacg attcaattta
    16861 gaaaagaata ggtatctttc tcatttctat cgctatactc cctcaggaga agagctacca
    16921 gaaaaacatg acaaacgcat ttcgaaaaag attttttata gtggacttgt atacctccct
    16981 ttagagtatt tagtaacaag agtaccagaa gttttcaacc gattggaaat tgttaaggga
    17041 agcttacaag gaataaatct gtttcaatta caggaaatgc cacaggtctt tcattttgat
    17101 tcagctggtt tttggaatcc atttactcct acaactagtt tgggtacacg tttagcaatt
    17161 tcgacagttt gtgtggttcc agctgtactt atgggattaa aaatggacat gtggtttcgt
    17221 cctctagcag atggacaaaa aggggataat cgattaatga ccttaaaaga aatccaacag
    17281 atttatccgc aaatacctga aacaaaaact acttttccag gttatggagg tattccaatc
    17341 acacattaca aaaaatattg gtacattcaa acagatactg ttaatacgtg tattgttggt
    17401 acctctcgat cagggaaagg tcagattgaa gtgttagcca ccattgacaa tttatctcgt
    17461 gcagaaagac aaagttcaat ggttgttaat gatccaaaaa cagaattata tgttgctagt
    17521 aaagatgaac tagaagctag agggtatgat gtgtatgcct ttaatatcac cgatccactg
    17581 caatcaatga gtgataatcc attggcactc attgtgaaat actggaagcg aggagacata
    17641 gacaccgcaa cacagttaac caatactttt acgaatacga tttattatga tgcaaatgct
    17701 tcagacaata aatactttaa cgaaaatgcg cagaaagctg tcaatgcgct tatttttgcg
    17761 ttattggaac aagcggatca aacaggggat tactccaaat taacaccaaa taatctcgtt
    17821 gaactacttt cagaaattgg cggttttaat tattatccag atcctgagaa tgaattcaag
    17881 cagctcaatg ccttagatga gttcatgaat cagttacccc ctggaaatat agcaaaaaaa
    17941 caatatgggg caacaaaaat tggatctgat aaagcgaaag ggaatatctt gtctacggca
    18001 attacaggat tgaatccatt taccctaacg aaaattgcaa agatgacttc tcaatcaacg
    18061 attgattata aatcggttgg gtttccaaaa tacttagatt tgaaattaca tgaaagtctt
    18121 ttaaatcaac gagtcgtagt agaattttat cataagggaa agaaatttca tgaagaacgt
    18181 gtcaaagtag gctataaagg attttgtgaa atcaattttg attgtcattt agttgcaggt
    18241 gatcaagttt gtttaaaata cgatcatcaa ggaaaagaat atgtggcttg gtatgaattt
    18301 tctcaaagag tattgaaaga cgaagagggt aatatcgttt atcgaaaaaa acgaggagaa
    18361 acccatttgc cagaattaga aaaagaagcc gttctaacat tagatgagga taaaagtaac
    18421 ttattagtag agtctattcg gatgcactat accgataaac caatggctat ttttatgctt
    18481 acacctgatt atgatccatc taaccatatc attgttagta tttttcttag tcagttatat
    18541 aaagaattat ctacgcaatg tgttaagact aaaggagata aatgtcaccg tagaattcat
    18601 tttattttag atgaatttgg gaatatgcca ccaatggata atatggaagg aatcatgacg
    18661 gtcacagctg gccgaaacat gttgtgggat ctgtttattc aatcttatca gcaactttat
    18721 gcgaagtatg gagaacaaaa cgcagcgatt atcaaagaaa attgtcagac acatatttat
    18781 attatgtcga cagatgataa taccatcgaa gaattttcta aaaaggtggg gaataagacg
    18841 gttgagcaag aaaacagtac catcaattca ttaggtatga atactcatat caatcgcaat
    18901 gttgattcag atcgtatctt aactcctgaa agaatttcta cttttttaga aggtgaaacg
    18961 attgtactcg ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg gccgtatcct
    19021 attttcaatc atggggaaac gaatcttcct tatgcttatc aatatattga agatttcaat
    19081 ccatcgtggg atatcaatga tatagacata cattgtacac atgcggactt ggatcttaag
    19141 gctttacaaa ttgattatga ttatttccta ccagatgaat atgctagaaa tctctatcat
    19201 ggactgcatg atcaattaga agaaaaaaac gaagaaaata tgttttataa aatcgttcaa
    19261 tctcttgaga atgagacggc taaaaaattg ttaattcaat tttacgaaga acaagcagca
    19321 gatcgacttc aagaatatgt tgaaaagttg atcaaaaata aagaaatttc tgaaatgatt
    19381 gggaaacggt tgttattgga atctgaaaag gtggtgaaag aatgatcatt ggaggaattt
    19441 tttctggcat agccgactgg tggtcaggct tattcggaga aggggatgca aaccccaaaa
    19501 ccgtaacgaa agtcttacaa gattatggta attatctgca gtatcaagat gtatttagtt
    19561 ggatttggaa cacttttctg tggggattga ttaaaggatt atataatctc aatactttac
    19621 tagaaaaaac catttatcaa agctttgatt taaaagactt attgaacgcc gctggggtaa
    19681 atgcactatt tcaaagtttg attagtaagg tattggctct tttttgtgtc gtcacattaa
    19741 tttatttagg cttgaaattt tttatgagta aacacccacc aaaagtcaaa aatatcttgg
    19801 ttaatttatt tatggccatg atcttgattt ttggtggtag ttctttaatc gatgaagggt
    19861 taaatatcag taaaatcttt tacggagacg tcactgcagc aaataaagca gataaaacgg
    19921 gatcacctgc ttttcaaatt attcaaaata atgtgatcga tgtcagtacg ttactagcaa
    19981 ctgacccgaa taaagtagaa tccctaccaa cagataaaag aaatgcttta acagcgaaaa
    20041 attttaaaac agcgaatatc aatgggatta ttactcctga tcaagccgct gagatggcgg
    20101 ataacgataa aagtggattg tcagatttac aaaaggatcg gttaaaggca ttacaatacc
    20161 gattggaact agatgataat gggaaagaaa ttcctgttca aattagtgac gaaggcttag
    20221 caaaatatgt ctatacttca gggtatcgtc gatacagtag taaaccaggt attatcttag
    20281 ctggtgaatc ttctttggcg gtggcctatt tatttatcct atttacgatc gttacttgca
    20341 ttattgagct ggtatttaaa aaattctatt tagtgattgc cgcgtcaacg gactttgaga
    20401 caggccaacg gatgaaaagt gcgatagaag atatttctca atccttcttg ttattggcgt
    20461 ttacgatttt agaattacga atctatacat taatgctttc aggtatcggt gatttgcatg
    20521 cggcaggaaa gatcaatgga ttcttgtatg ttgttgcttt aattgtgcta actattgcgc
    20581 tattcaaagg ctctcaatcg gttacgaaga ttttcggtgt tgatacgagt ctgaaaaacg
    20641 gcagtaattc tcttcttagc atgtttgcct taggaaatat tgcgaaaaat gtgggtagcg
    20701 gagctaaaaa ccttgcaagt gctggtaaga gtggtttaag ccatttaaat gatattcgta
    20761 aaaatggatt tggccatcga aaagatgtta cccctgataa cggggaagaa caatcagacg
    20821 ataaagctac aactgtacct aatagtaaga aaacagtttc cgaaatgttc gggaaagcga
    20881 aaaatggtgt ttctaaagcc gcagaaggtc ttggttacgt caatgaacgt gggttaaaag
    20941 gaacagctga agatcttaca gaaaaagcca aagacacctt agaagagaaa ttagacggca
    21001 cgagagctaa agaattggct gaagctgtga agaatccatc cgaagcacta gaaaaacaaa
    21061 aagaaaaagc ttcagaagtc aaagaaggac taaaagatgc gtatggtgaa ggacaagtta
    21121 atgcagcagt aaaagctaat acaacaaccg aaaaagaaaa tcaagaaggt atagtgattc
    21181 cttcagtcaa agatcaccaa gaagcgttgc actctaatga aaattcgatt gaagagaaaa
    21241 attccataga agaaagaaat tcagaagaag gaaaaaatgg acagttacca aaaactgatc
    21301 cagaacaata tcgctataaa cctaacagtg accttgaaaa tgagaagcga atttcagatt
    21361 tatcaaaagt aaatcaagaa gacaaagcag aaaatgaggg acagttacca aaaactgatc
    21421 cagaacaata tcggtataga tctgttaact ccgaaaatga aaaacgcatt tcagatttat
    21481 caaaacgaaa tcaagaagta caaacggatg aggattccat cgttctgcca aatgataatg
    21541 gaatcgaagt tggaactgaa aaatcaacga aaattggttt acctaaggca caaaatttac
    21601 caatgaaaga tagtcgtttg aatcaattca aggataatga tcaaagtttt gttcatcaat
    21661 cacctgcagg tactgttcaa atgaaaccaa gtcgtttgcc tgaaaatgga aatctgacaa
    21721 ctgtttctta caatttatct gaaagtggtg agacagggaa tgcgtccgtt caaaatgttg
    21781 tgacaagaga agctgcacca aaacctattc aaaacgttca tacaggttac aagccagcaa
    21841 atgtggcatt tactgcacca acaaaaataa atgtgacaag agaaactaaa aacaggaaca
    21901 atgcattgaa agaacaacta ttgagggata tcaaacagga tatcaaacca taaaaggcct
    21961 acaagttagg ccttttcttt atatgaaaag gagaaatcaa ttatggataa atatataaac
    22021 atgaaaaacc ctccaagatt tagtggggaa tggaagtttt tcgccttttt ctttttggat
    22081 ctattaacgc ttggtggttg ctttttttgg gcgaaaatag tcaataacct tgtccattta
    22141 ccactctttt ttgcactctt gaattatggc ttatggattg cgatagctct agtgattgtt
    22201 tggcgaccct ataataaccc aggtcaacga caaatttttg cgattatcga gtctattttt
    22261 tttacggaca agaaccatta tcatgcgtta gatcctaacc gaaattatca tggtacgtat
    22321 aaggaggatg aataaatgcg agtcaaagga caaaatttag ttgataagaa atccattttt
    22381 cagtttggaa aaccagaaga acgatctatc ttggatttat ttccttataa atcctgtaca
    22441 gaagagggag tcttagttac taaagaagat cattttcaac gttattatcg tgtagtttct
    22501 acagacgtgg aaggattaaa tgaacaagag aaactagaac gaatgaatca gttgaccacc
    22561 gtcatgcgta cgtatgtgcc aacaattaag ttagttagtt tgaccacaga gacggattta
    22621 acggaacaaa tcgtgcaaaa gcggcaatta ttagataaaa atcgtatgga acagtcctta
    22681 ggaagaaatc tacaacgatt aaaaaaatat gaaaagaaat tagtcgaaga aatccaggaa
    22741 ttgaaacgag cagaacaaga acgtcctgac ttaagcttct ttttcgtcat tgaaggaaag
    22801 acaatcaagg atttacaaac cagaaaccgt cagttaatgc gatctagtgg cattttaggg
    22861 ttaaaaccac tctacaaaaa agaattgatc aagattctgt atcgaatgaa caatatgaat
    22921 gatgagtagg aatggagaag agagaatgaa atatccgttg atatggttag aaaaagatgt
    22981 tccgaaggaa gagaccacta gaaaatttcc tcacacttta agcctgggta agttgggaaa
    23041 agggcaatct tttaaccaag taaagaatca aacaagaaga ggtgactgta catgttagaa
    23101 gcaattaaaa cagctttttc accacaaaat gatcaaaaag tacttgatcc attaaagcaa
    23161 ctaggaaatc gggaaataga aattttaaaa tccaaagggt atgatttaga ttttttaaaa
    23221 caggtgatgc cacaaggtgg gttaaatttt gatgaagaat atgtgacgta tggtaatggt
    23281 tattcacgat gtgtgacagt ttatcactac acaaaaaatc ctacgctttt ttggttggcc
    23341 aatctcatga acaatcctca tacagtggcc acactcgatg ttagcacaga tgaaaagcaa
    23401 aaagtgattg atcaaatcaa ccgcgcatta gatgaattaa atgatcgatc agataatgaa
    23461 cgaaaagcga ctgaccgaaa taaaagtgcg gaagaatacc tggaattagc ggaatatgcc
    23521 cgaaaattga cttctaatgg ggaaatttct aaacaagtca aagtgagagt acatgtttac
    23581 ggaaacacga ttcaagaagt agatgaacga gcgcaggaac tgatcgaaaa tctcaaaggg
    23641 atggattatc gtgcggtgac ttatctgcat aaaaccaaac gagattggca atcgatgttt
    23701 accatgttcg atgaacaagc tgaatggttc ggaaatatca aagggcaatc actaccatcg
    23761 ttaaacattg gcggaggttt cccgttccac caccaaacgc taaaagatcc aggagggaca
    23821 caatatgggg aaaccatgac aggcggccca tttgtctgga atgcgacgta taaaaatagt
    23881 gtacggttgt ctactaatac cttgcttcta ggaatgatgg gagctggtaa atcgaccgtt
    23941 cttaaaatga tcgcggaagc ccatttagct gcaggagact ttatttgggg ctttgaaaag
    24001 ggaaaagact ttattccttt cttaaaggaa tataacggta ttatggtgcg tttagacgga
    24061 tctgacggaa tgatcaatcc tttagagata tttgccacac ggacgtatga tgaagcatcc
    24121 agtttatatg acgatggatc agtcaaagat ttgaagatca atgaagcagc gtcctatcaa
    24181 acacacttag acaaagtggt ctatcaggta cagttagtga gtcctcagct aaaaggaacg
    24241 atgaaagcag aatttaagac ctacttaaat cgtttttatg aagagtatgg aacagtacca
    24301 cgcggcttta cttcctcaaa ttcgcgttca aatactgata cgcaagtcac aggtaaagat
    24361 ccagaagctt atccgacgtt caaagaattt ttagactttt taggacaact ggaattacca
    24421 ggtgcttccc aagagaaaaa gaatcgaaaa gaagaaatgg aatccatcgt agaatcctta
    24481 tgtgagacgt atggcatgat ttttgatggt catagtacga ttcgacacct tgaccaacaa
    24541 cagttggtct tttttgacat tgactctatt acaggcttag atcgaggggt acaacaatgt
    24601 caaatgtaca tggctatgac gttgatttgg aatcaggcat tgattcaagg aagaagacaa
    24661 cgacgtttaa ttaaagaaaa acaaatggaa gaacaagact tcaaacgctt cttggcgatg
    24721 attgatgagt gccataatgc gattaatcca gattttattg aaacggtaaa ttatgtcaca
    24781 aactttcaac gagaaatgcg taaagtagaa gcaatgactg ttttagctac acaatcccca
    24841 caagaaatgg tgccagaaaa tatgtcttct gataacttct cgaaactgaa gaaagtcttt
    24901 gaattttctt ctacgaagat ctttatgcgt atggatgaat ctattttacg tcatatagaa
    24961 ggattagtgg gtaaatcaat gacgaactca gaattagaat ccattcctca acaaaatcaa
    25021 ggagacgctg tgatcaattt tggctcaaaa gaaaccattc gtgttcattt tagtccgaac
    25081 aaacgtcaat taaaattgtt tgatggagga aaataatgga aaaccaaaat gaaagtttaa
    25141 tcaagcagta tgttaagcgt cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt
    25201 ctgcaggttt gattactatt ttgatcactg tgttcgtgac cctatttcta atacttgcgg
    25261 cgggctcgat tgataattcg gattcagatt caagcagtgg tggcgaagct tttacaggag
    25321 aatattcgga aggcttaccg atttataaag aaatcaaagg aagaggccct ttttctgatg
    25381 agattgcgca atatgccgta ggtgcagcag tgaaatataa attattacca agtgtgatcc
    25441 tttcgcaata cggttacgaa tcagcgtttg gtacatcggc gtccgcaaga aatgatctca
    25501 actattttgg gattacttgg tttgatggtt gtctgtttcc taaaggaact gcacgaggaa
    25561 ttggtggcat tgaaggtggc tggtacatga agtttcccaa ctcaaaagct gcattttcct
    25621 attacggctt tatggtagcc acacaatcca attttaatgc ttgtgtcgga aataaaagcc
    25681 ctggcgcaag cttattgatc ttaggacgag gcggttatgc cgctgcagga attacggaag
    25741 atagtccgta ttatacaggt tgtatgtcaa ttatcacttc taataaatta accgaatatg
    25801 atgaattcgc catcaaacat tggggtgaag gtggcaataa caacggcacg attactggtg
    25861 aatggacgaa tccattccct ggcagcagtt tagataaaag ttccttcagc ggtggtcaat
    25921 tatttggaac gaatccaggc ggtgaattcc gtccgaacgg cttccatgat ggattagatt
    25981 ttggttcagt tgatcatcca ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt
    26041 acgttggcaa tcctggtatt tcaggtttgg gcgcgtgtgt gatcgtgatt aattacgatg
    26101 gcttgaatat ggtgtatcaa gaatttgcaa atagtacagg aaattctcga gtgaaagtag
    26161 gcgatcaagt gaaagttggt caagtaattg ggatacgaga tacagcacac ttgcatttag
    26221 gatttactcg aatggattgg cgtcaagcac aaggacatgc attcattgat gatggtacat
    26281 ggatcgatcc gttaccattt ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg
    26341 aaaataaaac gttaaaaatc gtcttaacag ctgcaattag cgttagttta atcggcaata
    26401 ttgcgttgtt ttggcagctg aaagaggaac aagcagcaaa tacctataaa attgaagaag
    26461 tggctaaaaa cgcgtctgag aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg
    26521 acaagttaaa tcaaaccatc aatgagaaaa accaagaaat cgatcagctg aagcaacaag
    26581 atggcacaaa agcaaaagag gaagtaactg aagcacaaag aaagacggca gaagagttcg
    26641 gtcaattggc cattgataaa tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg
    26701 tcgcaaccca agaagtgatc gacaaattaa gtcctacaga cgatgatcac caacatgatg
    26761 attacggaag ttactccttt acattaggtg acgtgcaaac ttacgctcaa acaagcagtg
    26821 taaaagagga acaaaatttt gtcgtatttg ttgattatac aatcggcaat ccgcaattca
    26881 aagatgtcaa accacaaaaa ataaaaggtg gattaactgt cactgaaaaa caagtagatg
    26941 gtcaatggaa agtcatagac tttagttatt tcactcgtta ggaggaagaa aatgagtaag
    27001 aaaaaaggag caagacaacg tttcaaaaaa cgaaaaagtt atcttcaatg tctgggaatc
    27061 tttatgattc tcggcttttt gattcttgct agtccgattt atacgaatca gaaggtagat
    27121 ataccagatg aggtaagcac gaacgccttt ttaaacacga aagagctgac aatggtttca
    27181 aaagaaaaag gagcgaacca gcaattagtt tttgaatttg cgatcgatga tgaagatcaa
    27241 agcttgttaa aagaactggc taatcttaat tatcgcgtag aagtaaagac agctaaaggg
    27301 gattatcaag caatacgagc caaagtgatt aaggtgtctg atgattattt tgcagtgaag
    27361 ctaacgcatg taccagaaga atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt
    27421 gatccaaaag tggatatgca agaaccacaa gatctgattt attacgttca cgaagataag
    27481 gttaaagatc aagtgaaaga tgaagattat aaacaacatg cgatcaatta caaagtgaaa
    27541 ggatacaaaa aagaacaaaa agcagcacaa aaacaaatgg aatccttcca agcaaccatt
    27601 gatttgaatg aagaattagt gaagaagcta aaagatcaat tgccttatca agtcgcagat
    27661 gatcaggaaa atactaaaaa caagattaat agctatcagc aagaaattga aacatcgaaa
    27721 acgcaactga aagatcaaga agagatcatt caaaaacttc aagaaaaaat tgatcgtatg
    27781 acaaaagaaa agatttagaa ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct
    27841 gacaaagata ctttcgctga gcagaaagta ttgattaaag cctatctcaa agttgtaaaa
    27901 gctaaagaaa tatgtcaaga agtgaattta tctaacacaa caatatctga gttgaagaat
    27961 ggtaggaaga gtttagatac cttaaaattc agatcatttg aagctctata tatagctgca
    28021 aaaaaatatt ttacggctga aagaaaataa aaacaggagg atttccatga atataaaaga
    28081 aataattaac tacttacaag aatttgaagt taaaatgact gcaccaggat tacgagctgc
    28141 cattagaaca ggctagctgt ctttatcatg tttagaatct aatcatttgt taatgagatg
    28201 ggagaaaaca ttaaccacga ttctaacaca aacatcaagc acataaaaca tttaaaact
//
LOCUS       DACDCJ010000032        28002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDCJ010000032 DACDCJ010000000
VERSION     DACDCJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.032"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(414..1358)
                     /locus_tag="IV038_001364"
     CDS             complement(414..1358)
                     /locus_tag="IV038_001364"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8341593.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1654..2598)
                     /locus_tag="IV038_001365"
     CDS             complement(1654..2598)
                     /locus_tag="IV038_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8341594.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2819..4033
                     /locus_tag="IV038_001366"
     CDS             2819..4033
                     /locus_tag="IV038_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8341595.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4055..6070
                     /locus_tag="IV038_001367"
     CDS             4055..6070
                     /locus_tag="IV038_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAP8341596.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6153..7526)
                     /locus_tag="IV038_001368"
     CDS             complement(6153..7526)
                     /locus_tag="IV038_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8341597.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7552..7836)
                     /locus_tag="IV038_001369"
     CDS             complement(7552..7836)
                     /locus_tag="IV038_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8341598.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7838..9853)
                     /locus_tag="IV038_001370"
     CDS             complement(7838..9853)
                     /locus_tag="IV038_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP8341599.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10032..10337)
                     /locus_tag="IV038_001371"
     CDS             complement(10032..10337)
                     /locus_tag="IV038_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP8341600.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10426..11457)
                     /locus_tag="IV038_001372"
     CDS             complement(10426..11457)
                     /locus_tag="IV038_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP8341601.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11664..12539
                     /locus_tag="IV038_001373"
     CDS             11664..12539
                     /locus_tag="IV038_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8341602.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12691..13533)
                     /locus_tag="IV038_001374"
     CDS             complement(12691..13533)
                     /locus_tag="IV038_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8341603.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13625..15502)
                     /locus_tag="IV038_001375"
     CDS             complement(13625..15502)
                     /locus_tag="IV038_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8341604.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15499..16920)
                     /locus_tag="IV038_001376"
     CDS             complement(15499..16920)
                     /locus_tag="IV038_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP8341605.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16923..20312)
                     /locus_tag="IV038_001377"
     CDS             complement(16923..20312)
                     /locus_tag="IV038_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP8341606.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20569..21960
                     /locus_tag="IV038_001378"
     CDS             20569..21960
                     /locus_tag="IV038_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8341607.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22033..22680)
                     /locus_tag="IV038_001379"
     CDS             complement(22033..22680)
                     /locus_tag="IV038_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8341608.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22688..23374)
                     /locus_tag="IV038_001380"
     CDS             complement(22688..23374)
                     /locus_tag="IV038_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341609.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23380..24453)
                     /locus_tag="IV038_001381"
     CDS             complement(23380..24453)
                     /locus_tag="IV038_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8341610.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24675..25349
                     /locus_tag="IV038_001382"
     CDS             24675..25349
                     /locus_tag="IV038_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8341611.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25354..26694
                     /locus_tag="IV038_001383"
     CDS             25354..26694
                     /locus_tag="IV038_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8341612.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26843..27829)
                     /locus_tag="IV038_001384"
     CDS             complement(26843..27829)
                     /locus_tag="IV038_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP8341613.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8172 a         5282 c         5068 g         9480 t
ORIGIN      
        1 aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg
       61 caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa ttgaatgata
      121 aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga atcattgtat
      181 ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta
      241 ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt
      301 aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca
      361 gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat
      421 acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct
      481 tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca
      541 cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca
      601 gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag
      661 taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc
      721 acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta
      781 ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata
      841 tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga
      901 gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc
      961 caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat
     1021 cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa
     1081 tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca
     1141 tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata
     1201 ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact
     1261 aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct
     1321 gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata
     1381 actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat
     1441 tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata
     1501 aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa
     1561 taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt
     1621 ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg
     1681 agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat
     1741 acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc
     1801 gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt
     1861 actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt
     1921 aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc
     1981 tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata
     2041 gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc
     2101 aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc
     2161 ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa
     2221 atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc
     2281 ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact
     2341 ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc tatacatata
     2401 attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact
     2461 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2521 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2581 tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt
     2641 tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta
     2701 tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt
     2761 gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat
     2821 gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc
     2881 tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt
     2941 atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat
     3001 tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat
     3061 tgcatcgatt tatggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc
     3121 aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc
     3181 tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg
     3241 atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata
     3301 taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     3361 tacacctgaa acaactgaac ttcaatcaca tggcacagcc ccaacaagac aagtaatcaa
     3421 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     3481 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     3541 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     3601 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     3661 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     3721 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     3781 gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     3841 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     3901 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     3961 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     4021 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     4081 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     4141 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     4201 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     4261 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     4321 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     4381 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     4441 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta
     4501 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt
     4561 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga
     4621 atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta
     4681 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     4741 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     4801 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     4861 atcagaagaa accttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     4921 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     4981 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     5041 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     5101 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     5161 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     5221 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     5281 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     5341 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     5401 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     5461 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     5521 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     5581 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     5641 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     5701 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     5761 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     5821 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     5881 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     5941 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     6001 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     6061 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     6121 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     6181 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     6241 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     6301 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     6361 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     6421 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     6481 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     6541 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     6601 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     6661 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     6721 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     6781 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     6841 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     6901 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     6961 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     7021 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     7081 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     7141 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa
     7201 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     7261 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     7321 aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact
     7381 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     7441 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     7501 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt
     7561 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     7621 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     7681 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     7741 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     7801 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     7861 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     7921 aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta
     7981 aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa
     8041 gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg
     8101 cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     8161 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     8221 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     8281 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     8341 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     8401 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     8461 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     8521 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     8581 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     8641 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     8701 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     8761 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     8821 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     8881 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     8941 agacattcat caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc
     9001 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     9061 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     9121 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     9181 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     9241 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     9301 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     9361 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     9421 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     9481 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     9541 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     9601 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     9661 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg
     9721 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     9781 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     9841 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     9901 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt
     9961 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
    10021 atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct
    10081 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
    10141 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
    10201 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
    10261 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
    10321 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
    10381 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
    10441 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
    10501 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
    10561 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
    10621 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
    10681 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
    10741 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
    10801 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
    10861 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
    10921 atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt
    10981 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
    11041 taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
    11101 ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc
    11161 ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat
    11221 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
    11281 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
    11341 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
    11401 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
    11461 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
    11521 atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt
    11581 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
    11641 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
    11701 ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct
    11761 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    11821 tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag
    11881 ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg
    11941 aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa
    12001 aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg
    12061 cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga
    12121 ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt
    12181 ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca
    12241 tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga
    12301 ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc
    12361 aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg
    12421 tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact
    12481 tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg aaaacttaag
    12541 aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc
    12601 ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc
    12661 gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg
    12721 acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag
    12781 gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata
    12841 aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt
    12901 taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc
    12961 taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa
    13021 agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg
    13081 atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa
    13141 taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac
    13201 attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt
    13261 tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc
    13321 taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc
    13381 tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc
    13441 gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag
    13501 cagatctaga agtttccttt tctttggttt cattcgtgtt ctccttgtga tgactgtttg
    13561 ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa
    13621 aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga
    13681 ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt
    13741 tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt
    13801 catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt
    13861 cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg
    13921 ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt
    13981 cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag
    14041 caagtgtttg atctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa
    14101 cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt
    14161 tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa acaaatttca
    14221 gcgtaccacc aggagtgagt gaaggaatat aattcgagtc aactgaaacc gtgaatccgt
    14281 ttgtttgttc agtgacagaa taatttgttg gatcgatttc tttatcccca tcatacaaga
    14341 catacgcata cgttcctgaa gtttcatgat caaatgtcaa agcggcgtca tgagtatcaa
    14401 tgagtttgaa tgtcgtgtac ttgttttgtg ttccttcttt atcagcgata cctaaaggaa
    14461 tattgacaga gatctcatat tggatttttt ctccgattgc gacatctttt ccattcaatt
    14521 gaggtgttgt tttatcaact ttggacgtat catttttgat tgtcttttct acaggagttt
    14581 gactatttgc caagatctca aacggcgtgt ttgtttgatt atcaatcatt tccgcattat
    14641 caggagcttt tacttcttct aaattatatt ttccaacctc taaatgattg acgatcaact
    14701 gacctttttc aatcgttgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag
    14761 taatgaaatg tttggctttg gtttgatccg ttgtccaagt gtacaatcca tctgtgacac
    14821 tttggatgta ttttttgacg cttggtcttc cttcttcttt agaaataata aattctgctc
    14881 catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac
    14941 cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag
    15001 agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag
    15061 ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt
    15121 ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg
    15181 taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc
    15241 gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt
    15301 ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat
    15361 cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt
    15421 ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt
    15481 ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat
    15541 tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt
    15601 ttttggaaga aatggacggc tattttcttt tttattgact actttcaata tactgtccgc
    15661 ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat
    15721 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
    15781 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
    15841 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
    15901 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
    15961 tgtatataca gagactttct ttgtactgat accaaaacca aaatccagtg ttccgttgtt
    16021 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
    16081 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
    16141 ttttttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
    16201 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
    16261 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
    16321 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
    16381 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
    16441 aacaactacc atattctctg ccttttcttt gacgacttct ggtgctttgg attcaatgaa
    16501 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
    16561 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacgtaccgg aagacgcacc
    16621 gttttttgcg atctctgttt gtgcttcttc taccgttttg cccttttccc gtagatggta
    16681 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
    16741 taataacgct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
    16801 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctgcctgagt
    16861 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
    16921 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
    16981 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
    17041 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
    17101 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
    17161 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
    17221 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
    17281 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
    17341 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
    17401 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
    17461 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcgg
    17521 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
    17581 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
    17641 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
    17701 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
    17761 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
    17821 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
    17881 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
    17941 tctcctgtta atttgaagac agcaccgatg agattctttt cacctgtaga ggaatttttc
    18001 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
    18061 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
    18121 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
    18181 gataatttgt caatgtctgc acgttcccac gaatttgtag tatctttagc tggtttaatg
    18241 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
    18301 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
    18361 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
    18421 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
    18481 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
    18541 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
    18601 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
    18661 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
    18721 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
    18781 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
    18841 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
    18901 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
    18961 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
    19021 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
    19081 ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt
    19141 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
    19201 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
    19261 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
    19321 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
    19381 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
    19441 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
    19501 ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
    19561 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
    19621 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
    19681 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
    19741 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
    19801 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
    19861 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
    19921 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
    19981 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
    20041 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
    20101 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
    20161 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
    20221 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
    20281 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
    20341 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
    20401 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
    20461 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
    20521 gagggagaaa aacatatgat caggttgttg gaatgaaact atagaaagat ggaaaaaatc
    20581 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
    20641 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
    20701 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
    20761 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
    20821 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
    20881 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
    20941 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
    21001 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
    21061 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
    21121 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
    21181 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
    21241 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
    21301 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
    21361 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
    21421 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
    21481 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
    21541 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
    21601 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
    21661 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
    21721 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
    21781 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
    21841 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
    21901 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
    21961 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
    22021 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
    22081 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
    22141 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
    22201 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
    22261 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
    22321 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
    22381 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
    22441 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
    22501 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
    22561 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
    22621 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
    22681 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
    22741 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
    22801 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
    22861 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
    22921 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
    22981 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
    23041 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
    23101 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
    23161 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
    23221 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
    23281 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
    23341 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
    23401 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
    23461 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
    23521 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
    23581 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
    23641 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
    23701 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
    23761 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
    23821 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
    23881 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
    23941 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
    24001 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
    24061 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
    24121 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
    24181 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
    24241 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
    24301 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
    24361 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
    24421 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
    24481 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
    24541 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
    24601 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
    24661 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
    24721 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
    24781 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
    24841 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
    24901 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
    24961 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
    25021 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
    25081 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
    25141 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
    25201 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
    25261 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
    25321 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
    25381 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
    25441 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
    25501 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
    25561 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
    25621 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
    25681 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    25741 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    25801 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    25861 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    25921 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    25981 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    26041 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    26101 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    26161 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    26221 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    26281 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    26341 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    26401 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    26461 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    26521 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    26581 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    26641 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    26701 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
    26761 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    26821 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    26881 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    26941 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    27001 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
    27061 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    27121 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    27181 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    27241 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    27301 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    27361 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    27421 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    27481 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    27541 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    27601 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    27661 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    27721 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    27781 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    27841 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta taggagacga
    27901 ttgttttcta caaatattta gattggaatt tgattaaaac aatttaattg aaatttgaaa
    27961 acatttatgt taatgaaagt catattctaa taaaatgtca tt
//
LOCUS       DACDCJ010000033        27877 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDCJ010000033 DACDCJ010000000
VERSION     DACDCJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.033"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(50..277)
                     /locus_tag="IV038_001385"
     CDS             complement(50..277)
                     /locus_tag="IV038_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341614.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(444..2414)
                     /locus_tag="IV038_001386"
     CDS             complement(444..2414)
                     /locus_tag="IV038_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341615.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2429..3295)
                     /locus_tag="IV038_001387"
     CDS             complement(2429..3295)
                     /locus_tag="IV038_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341616.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3285..3518)
                     /locus_tag="IV038_001388"
     CDS             complement(3285..3518)
                     /locus_tag="IV038_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8341617.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3530..5686)
                     /locus_tag="IV038_001389"
     CDS             complement(3530..5686)
                     /locus_tag="IV038_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341618.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5706..5927)
                     /locus_tag="IV038_001390"
     CDS             complement(5706..5927)
                     /locus_tag="IV038_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341619.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(6037..6375)
                     /locus_tag="IV038_001391"
     CDS             complement(6037..6375)
                     /locus_tag="IV038_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341620.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6775..10416)
                     /locus_tag="IV038_001392"
     CDS             complement(6775..10416)
                     /locus_tag="IV038_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341621.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10419..12368)
                     /locus_tag="IV038_001393"
     CDS             complement(10419..12368)
                     /locus_tag="IV038_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8341622.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12410..13468)
                     /locus_tag="IV038_001394"
     CDS             complement(12410..13468)
                     /locus_tag="IV038_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP8341623.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13587..14042)
                     /locus_tag="IV038_001395"
     CDS             complement(13587..14042)
                     /locus_tag="IV038_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341624.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14626..15429)
                     /locus_tag="IV038_001396"
     CDS             complement(14626..15429)
                     /locus_tag="IV038_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8341625.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16410..17633
                     /locus_tag="IV038_001397"
     CDS             16410..17633
                     /locus_tag="IV038_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8341626.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17668..18435
                     /locus_tag="IV038_001398"
     CDS             17668..18435
                     /locus_tag="IV038_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8341627.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFKDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            18625..19023
                     /locus_tag="IV038_001399"
     CDS             18625..19023
                     /locus_tag="IV038_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8341628.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(19329..19724)
                     /locus_tag="IV038_001400"
     CDS             complement(19329..19724)
                     /locus_tag="IV038_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341629.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(19725..20102)
                     /locus_tag="IV038_001401"
     CDS             complement(19725..20102)
                     /locus_tag="IV038_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341630.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(20092..20487)
                     /locus_tag="IV038_001402"
     CDS             complement(20092..20487)
                     /locus_tag="IV038_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP8341631.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEDLLEK"
     gene            complement(20554..20739)
                     /locus_tag="IV038_001403"
     CDS             complement(20554..20739)
                     /locus_tag="IV038_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8341632.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(20821..21075)
                     /locus_tag="IV038_001404"
     CDS             complement(20821..21075)
                     /locus_tag="IV038_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341633.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(21349..21633)
                     /locus_tag="IV038_001405"
     CDS             complement(21349..21633)
                     /locus_tag="IV038_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341634.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(22127..22666)
                     /locus_tag="IV038_001406"
     CDS             complement(22127..22666)
                     /locus_tag="IV038_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341635.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(22779..23045)
                     /locus_tag="IV038_001407"
     CDS             complement(22779..23045)
                     /locus_tag="IV038_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8341636.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(23047..23268)
                     /locus_tag="IV038_001408"
     CDS             complement(23047..23268)
                     /locus_tag="IV038_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP8341637.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            23494..24312
                     /locus_tag="IV038_001409"
     CDS             23494..24312
                     /locus_tag="IV038_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8341638.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            24316..24612
                     /locus_tag="IV038_001410"
     CDS             24316..24612
                     /locus_tag="IV038_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341639.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(24794..25147)
                     /locus_tag="IV038_001411"
     CDS             complement(24794..25147)
                     /locus_tag="IV038_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341640.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(25167..26222)
                     /locus_tag="IV038_001412"
     CDS             complement(25167..26222)
                     /locus_tag="IV038_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP8341641.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            26438..26836
                     /locus_tag="IV038_001413"
     CDS             26438..26836
                     /locus_tag="IV038_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341642.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
BASE COUNT         8601 a         5026 c         4239 g        10011 t
ORIGIN      
        1 ctgagatcac ctttcaatct aaatacacgg ttcttttctg gtaacttatt catgcttcaa
       61 ccacctcata ctgttctctc atacctttga cttcattaaa aaaaactctc gctttttctt
      121 catcagtaaa gtttttatac aactgattac aataatgagg tttttgaagt ttctgaatac
      181 ctatactaac ttcaaatcct tcttcatgtt gaattaggat tacttctgtg tctctaccta
      241 gaaatattcg atctaaaatt tttatgtttg gtttcattat ttaacctcca ctataattat
      301 ttcactcaat aaggtttcac tttatgaaat ttctatagtt ctcttttcac aaaacgtacc
      361 atgttctcta agactgtttt tagtctcaaa tatacatgtt ttttcttatc ctttccttat
      421 acactcaaaa tcaaagataa aacttaaaga ataaactctc caataacaaa attattagtc
      481 agatccactg gtctcctgat ctgaaattta ttacttcttc ctgttgtcaa atactgtctc
      541 aaatcaacag aaagtttttg atcatcttct ccttttctag caggagcaac ttttttttca
      601 tacttcatta actctccact ggtcatcttt ccaatgatag tgtaattaaa atcactttca
      661 aaactttggt aaatttttcc atctgtattg aatatatcag ctgtttcaat atcttctttg
      721 tattcattgt cttctcctga accgattgta tcaaatagaa aggcaagacg aattccatct
      781 tcttcaagca ttgttaatct ttttttaatt agcccaacgg tttcaataga taacttatcg
      841 aagccgtgaa tcataacaat atcatctttt tttgcttgtg atgtgatgaa atcaaaggta
      901 tttaaaaatt gagcttccat aacattggtt tctgctctta gatcacctaa gttatagtaa
      961 aactgtaatt tgtcactttt accgatttct ttcagtgtcg ttctttgatt aaacatccta
     1021 gagaagctgt ctaatgctgt tctcaatgtg tcttttaata caataatacg atcgacttct
     1081 ttttcagtac cagatttttt ggcttttgtt tcagcattag tcaaatagct aataaaatcc
     1141 ccaagaactg gcattgtatc actactgttc accaaaagtt ttgaacgttc ttcataatta
     1201 atatcccaaa gtttctgact tatataaaag ttacctaatt cttttttcaa taaaactcta
     1261 gttgcagaat ctaatgttct gcctgctaac aaataaaaaa tctgagttag tttatcgaga
     1321 ttccgtctat ataaatcacc aatgtccttt tgattaccaa aaagctccaa agagttaaga
     1381 ccttcttcag ccaaattaac cttttctatt acactgttta acgaaggttt ggcaacaaat
     1441 gttctaccag ttggatcacc aaagtaatca aatttattta agacaatatg aaaagttcgt
     1501 ttatgatttg ccattgcatt attagcaatc aattgccccc aaagtgatga tgctgagatt
     1561 ttttcatcat agcctttaat tgtagcgttt ttgtgtgatg ccactaaaac tttacttgta
     1621 aaagaatcat ccagtgccat aaaagctaca ccacttgcgt agctagtaga catagtgccg
     1681 atagcccatc cattcgcatc atctaaacct ttgcgaattg catgatcatt tcctgcaaaa
     1741 tccgaactca tagacgtata ctcatagaca ttcccgattg gttcacttaa aagtctatca
     1801 aacagatcac gttgatctcc tccatatgac ataagcttta atccatacat tttttcatca
     1861 tacaaccgtt ggagtttaat tagctgttcg ctgattttat taggagaatc ggatactaat
     1921 aggatttcta gcttccaatc aataatttta tttgccatgc tgtctagtaa agatgctgta
     1981 ttcaaatcca taatccgcat aacagttttg ctaatttcag catctgaatt ttttccttct
     2041 aaattatttt cataactatt cgttgatccc ccaacattct cgtgcattat ttcttgttga
     2101 tctttttgtg acactggctt atctgcttca attagatagg cttttacccc ttctactctc
     2161 acttcaggaa tagctttaac catccaacca aatccataat ttctattcat tccataagta
     2221 ttcactaatt tcacaattgt tccccaccgc ttacctgtcc taatgtaaga tggtgtaaat
     2281 tgataaccaa ctaaagatgt taaatcacta acaaaggtat tattcactac ctttgaattt
     2341 ctagtttttt gttctttttt agtttttctc ttttttttga aatttaatag ttgtgatcta
     2401 cccataattt ccatttaatc tctcacctct atttcagtgc ataaatatca gataatattt
     2461 cttttgtttg tttagctgac aactgagtga cactataata aagtcctcta ctcgtatagt
     2521 tttctaaacg ttccacacct tcatccaata ccatcttaga ttgagatttc aagataagat
     2581 attggattac tgttgttttc acaccatcaa tatattctga tacaaatcgt tcttgctgat
     2641 ctattatgtc aaagactgct ttgttactcc ttgcatttcg tttttgtatt ttcatatttc
     2701 gagtttgtct ttccgtattc tgaggttgag atgaagtgat tttgatttca gctaaggaac
     2761 gatctctggt attttgatat tgtattgatt tcgattcctg agccataact tcttctggaa
     2821 aagcggttag tgatgtagcc ccatctagat tgaacattaa tgctacactc ccatctcgat
     2881 atacgattgt tccatcttgt ttcactttta aaatctctgt gcctcgaaat ggtttctttt
     2941 tccccataga tactcttgct gaattaacta ctgatttgaa cagtctaata gaaccagaaa
     3001 tcaatgaacc actagatgga tcaataaaca tatactggct aattagaata agaaatccaa
     3061 ttatcattaa taacgttgga acaaaaataa atttcagttt aatcgtatca gaaactaagt
     3121 aaacaattga tccaagaaca acgactgcaa caactatagc tgatacaatt aatctttttg
     3181 ctgtaacaga ataggtgcta ttgacatgct tttcaagcca ttcaactctg acaattgttt
     3241 cagaaggttt ttgatgtaag ttatgtttaa atccatagtt tgagttatct gccatcagaa
     3301 atacttcctt tcacgttcaa cttacggtaa acacctacag ttatcactat tgcaacaata
     3361 agcaagccaa ttaaagatat tgacttgctc attgtctcgt tcgtttgtgg tagttcattt
     3421 acctgataca cgagctgtgc ttcctgtaag tgctgagaag aggtcatgtt ttcttgattg
     3481 ttttgaatta ctggataaac tattttttct tctcgcataa tagccctcct tagttattat
     3541 cagataagtg agcttgaatt ttgtactgat ttccgtctgg agttttcaca actgcttttt
     3601 ctccatcaat agaaatcact tcagccttag ttgttttccc atcaatactg atgacaacat
     3661 ctttcccaac tttgaactct gtagttggtg ttgcttcata gctactattt gttgaagcag
     3721 agttcgtatt tgtcccaatc gaattttcca ctctcgaaat agatgtttga gtttgagcct
     3781 gtgcttgttg attattatga ctaataaagt aggacgatcc caagatcgtt ccgcaagcca
     3841 ttgcagcgac agaaatgata gtagccatct ttttacttgt aaaatcacgt tttgaacttg
     3901 attgattttc tgtagcaact gctaatgatt tcgttttttc tgccatttga gctagatcac
     3961 ttcgggaacg agataagtct tctcttaaag cttcactttg ttctgccaac ttttcgctat
     4021 ttaatttata accctctgct aaatctttga cctctttcat tcgatcttta tattcttcga
     4081 tagttttttg ttgctctaca agttttgttt ctaattcttt cgtgttgtta gctacctgct
     4141 tttcaacttc ttctttgttc gcaaaatatt tgacaaccac atctgttgtt ttctcttttg
     4201 tatcatggaa ctcagcttca ttttctgact ttgttttttc gacaatctct aaagtttttt
     4261 cttgcacgtc tgttaatgat ttttcaattt ctggtttcaa tgaattgata attcgttcca
     4321 ccacaacttc ttttgcttct tggctttgtt tcaatgcatc gtttttaata gattcaattg
     4381 cttcttggta ctgttgatca agactttctt cagttgcttt tctatattca tcttcttgtt
     4441 gtttaacttc tggtaaatgc tcagcatcat accgttgctc tagtttcttt agttcagcat
     4501 ccaaataagc ctgtttcgcc atatcataat ctttacgaat gatattggat ttttccatca
     4561 ctaaatcatc aattttagat gcttctgctt cgtagctctc tttagcaacc gatagtgtat
     4621 ttgtaaactg attttgacca tttaatgagg ttgcttcctc tatccgcaaa agctcatcag
     4681 ctaacatttg ctttcctttg aggatcaatt gctccttcgt cttatttaat tcactgttga
     4741 ttttattatt agcaatagag atgcgagcgt tcatactctc tttagctaaa aacagcaatg
     4801 gtttcaactc aggtatggat agcatctcct cgtttaaatc gacagggtca ttaatctcat
     4861 ctaaaactac aggttgaaat ttgtcagcaa tgatctgttc aaaatttttt tctaaaaaat
     4921 cagcatctaa gtttttcggt acatcacttt cagatgtgat tttttcagct tcataaacat
     4981 aattttcttt gtcactttgg gattcgggtt tttcatcaat ttttggttgt tggtaggttt
     5041 ctgtttgtat ttgtgtttct tctatgctta cttcttggtt ttctgcttcg gttggtttgt
     5101 tatcatccac aaaaagagct tctttttcat ctggtacatt ttctgctacg gcttcaaatt
     5161 caacttcttc agaagagttg aaggtctcag caattcgttc taatccatta gtagctactt
     5221 ctaattgctc tactgcgcta tagaagtctg tagcttcttc aacttctgat tcaaacacat
     5281 taggatcatc cggaataact aataattgac cactactatc cataaaacgg acacctgcta
     5341 atagcttctc tttaaatttt tctggtagat cagctacata attcaaactt gacttcaata
     5401 gaacgacata cttctcagaa tcgagaatag catcatcctc atctataact ccctcactgt
     5461 tgagcttttg tacatcttca attaccatat aatttttctt tggtaaatct ttggtcagca
     5521 acgcatcgaa ttctttccat acctttgctt tagctttttt tgccttcgct tctttttctt
     5581 cttttttgta cacttcgagt aattgtggct tcaaagagag tgcaactaca ggaactgttt
     5641 ctgccacata attaatagat tcggtaatat ctttgaattt tgccattatt gtaaaactcc
     5701 ttttttcagt caatagttct tttctgttta tctagcatat atacatatgc tttttcgaat
     5761 ttaataagcg tttcaagtct ggctttttca atctttttac tcccatttcg ataatcatag
     5821 aactggttaa cattcatatt cattatcttt gccagttcgg tacctttcag actgctcatt
     5881 aatacttcgt tggcgtgagc taacattttt tctctatcaa aaaccatctt acatttactt
     5941 tcttccctca cttctaaact acgcttggag cgtagttttt tggtaaaaag taaattgaac
     6001 aaatcggcat attatccaaa tttgttcaat tcacttttaa cctaacagtt cattttttgg
     6061 aaattttgaa actagataga taccaacaac tggaataagt gctgatatga tattagcttt
     6121 ttggttcaaa tcattacctg ttttagtacc cattgtgtaa agcataacgg ttgtaattac
     6181 aggaataata aagattgcaa taatcgcccc aacatatttt aatccatctc cccattttgc
     6241 atcaccaaaa aatttcattg cagtgaatcc tggtttcaac gaaaaaacga atgccaagaa
     6301 atacaaaaca acttgtacta aagttttcat atcaggcgtc attccgctaa ccaaattttt
     6361 aatagaatcg aacatataaa aacctccttt ttaatatttt taataagtta attatatcat
     6421 cgacaaatct tatttgttca cttgtcaatg atctaactaa gaaaaagacg tctcgatatt
     6481 tacatcttta tgttatcata ataacataaa ttttcgatga ttaaaagaga ttttccatta
     6541 aattatttca ctttttttat tggctaaaaa tgctggttta tcaagttttt gtttagataa
     6601 taattctgtt ctttaccagc ataagccctt tttaattata caactttgaa cagtaaatat
     6661 ttaattcatt tatagacaat ttaaaataat ttttttgcat aaatagaaat tttccttgac
     6721 atcttcagtt agaaatcaaa gtaaaaagaa ggagcaaatc aatgctcctt ctttttactc
     6781 gtcttcagac ttattttcag ttgatttttg attaccgtat tttacaatat agtccttgac
     6841 tgacatggta gatttcttca ctactttacc agttttggat gctcctgtag cagtagcctt
     6901 ggcaacctta gccgttcctt tacctatttt aatagcttcg tttttagctt cataggttat
     6961 ccctgctaga ctaccttttg gagcttttgc taaagcttta tctgctgagg tgtaaacatt
     7021 tttaccagtt tttgatagat attcaacggc tgatttagct gttgatgttt tcttggttga
     7081 ttgattagat ttccctgaat tatctgaagt agatttagaa ttatttgttc tattattttt
     7141 atttgtcagt tcaggttctc taattgattg attgtctttc gacattcctg atccaatatt
     7201 tgtttcatat ttatctgttt gattaacatt aggagtagga atgaactttt tcttcacatc
     7261 tgattgttct ttatccgttt cttttgttag cgattctctg tctttaagta ctgtgtcatt
     7321 ccgaatagca ctattatcaa agttgctgtt aagctctctt gactgacttt caccagatgc
     7381 tccatctcct aattctcgat gcactctgtc tgacacgtcc tcaccactca tttcattcct
     7441 cagttcttga tgagtttgat ctaaagtaat ctcgtcttct tcattctcag gaattgcaac
     7501 agcaattggc gtatgccgtt gttgttcgag ttctctattt cgatctgcca caagcgcatc
     7561 tctcgtctca atgatattcc catcctcatc gactaaatct ccattcacat tatatccatc
     7621 gctgtgttta ccaagtttat taccaaactc atcaacaaga tttccatctt catcaactaa
     7681 acgacctttt tcgtcaacta aacgtccgtt actatcatga ccaaaatcac catttagcaa
     7741 cctgcgtcca ttttcgtcaa caagatttcc atcttcatcg actaagcgac ctttttcatc
     7801 aattaaacgt ccttcgctat ccataccaat attaccatct tcactatctt ccaatggttt
     7861 accattagta tctataaggt ttccatcttc atcgaccaaa cgcccttttt cgtcaataaa
     7921 gttgccttta gcatctttac cgtatttact atcttttaat atttcaccat tttcatcgac
     7981 aaagtttccg ttttcatcaa ccatacgtcc taaatgatca atatattgtt gggagttatt
     8041 acgtagttga tcttgacgaa ctaaatcttt gaaagaggta ttattgattg tggaaccaac
     8101 tccgccaccg ttgaatgaca ttccttctgg tgtttcagga attactttat cagcaatgga
     8161 cttagcatca gcattgctat ctcccatatt gttcaaacgt tcagccattt tttgcgctcc
     8221 aactgctcca agacctcctg caaccatatc ttttactgag ctgttgccac catcataata
     8281 gggattttta gctccttggc ttttcttgat tgctttagaa acgccgtcca caactcgcac
     8341 agcatcattt ctattaccaa ataataactt agcacgttca tcaaacccaa acgctacttc
     8401 aagtggtaag ccaataagca tattaatcaa attatttact ttttcgcctt ttttaaagac
     8461 taaaagatat cctacaccta aaatcatagc agatcgaaca atatttgcta cgccatcgcc
     8521 aactaatgta ttaggaatga atcccacagc agtatcacca aatgcacgaa taaaagtaat
     8581 cagaaagata ggtaattgta gtccgatcag agatgttgta acaacaccta atgaaattgc
     8641 catcacacct gctcctgatg caagtgatcc tgttagaact acttcccaaa tccgaataaa
     8701 ttcacgttta aatgctttca cgatattcac attgaacatc gcccaagcac atgctaagta
     8761 aagaactgct aaaacgattg attcagcgtt catcgctagc cacgagccaa acttacccat
     8821 agctccatga ccaactaagg atgctgtacg ccattctgtt tttactgttg acatattctt
     8881 agcactgcct tgaattcctg aaggagcgat attatacgca taaaacttag ctcccgatgt
     8941 atcaagtgcc gattggagca ataaagctac cgactgtgtg ctaaaagttg cattgttagc
     9001 aacgccccaa ggttgagtag catcaaagtt atgcgctgtt ccttttccat caggcttgcg
     9061 attgtcgtct gtcacatttt gatttgctga ccaaatatat ccatttaaat tggttggttt
     9121 cttaattgca cttccttctt ttccatcccc taaattatag cctgtccaaa cagcaggcgc
     9181 tcttcccata taagcatcat tttcggcgac attaacatca ccggcataag tattaatatt
     9241 gaaagtatca ttcgatgacc attcttttaa tagagccata ccgactgaat tagaatctag
     9301 atcattattt tgtctggcat aactgctagt attgatcgct cttatcattg cagatttttt
     9361 agatggttca taatctgtat caacgaactt gatttcggat gtaggctttc cgtcaggtac
     9421 atcaccacca gtatcagatg atggagcaag attttgagta gaagcccaaa gtcttgtgtt
     9481 aatgatgtag tcagaaacaa catttgttgg tatatctcca tctttttttg ctgactggat
     9541 gccgttgata taaactccca atagcgatcc tgccactggt aagaatataa acaatacaaa
     9601 aatacgaaca atccattttt caagtactgg tgataatccc ttcgagttaa caccatcttt
     9661 cgacaataat ttcattcctt gataacctag tattgctgtg aaaaagagca tagataagct
     9721 tgctatcgct ttaaataatt cattatttaa tcccaaattg ttaagcaagc tagttaatgc
     9781 tttagatacc ggattagata catccgatcc accttctccc caccccataa gagaaaatat
     9841 attcatactc atgaaaaatt ttccaaggag ttcatttatg gattcagcaa tattagaaag
     9901 gattaaacta attgatccga taaaactacc aatcgtccta ccaatacttc ctagaatgga
     9961 tgatgcttga ctggcaatag catttcctga gactgtttca atatatccgt agtaactata
    10021 agttgctaaa gtagatgcta gttgagtacc tgcattaccc cctgcaatcg aattaccttg
    10081 tttagaaatg tcagaataag ggaaatcggc actgatacca ccttgtccta acaaattcat
    10141 ttgtccagtt ttagcggcac tatcattaac ttctttatct cccattagga tacgagcata
    10201 tagtgtccac attccttgtt ctgccggttt actttccgca tatgcaacgt tcttatttat
    10261 aactgtttct tgtttgctat cttgattgtc aggagttgga tcattcattc caaaaggagc
    10321 gcctaaagta actgctaata ctcctccaat aactagacct cttacgaccc atcgttttat
    10381 tctaaggttt tgtagtaaat atttcttgaa tttcatagct acgcccctcc aatcgaaaat
    10441 acaatcttgt tttctttact ttcagcaacg gaaggctttg caataatttc agcattagaa
    10501 tcaccttttc cttcaaacgc tccggcttca ctcaacttaa catcgaatat ttttgcgttt
    10561 ggataagtaa ttattgcttc agtaatcgct tcttcaaact cagattcaac taaatgcaag
    10621 atattccttg caccccctga tttagaatcc tcttgtgaag ccatgtcaaa aactaagtgc
    10681 atggagagat cgtctgcata aaaattttga ccatatactc tttctttcct aggtggaaga
    10741 cgtaaatcta tatttaaatg ttttcgtact aattcagcac tcttcaacat ttcattataa
    10801 gcaatttcca gaagagtttg ttcggataaa gcccaaaatg gaactattcg tccaaatcgg
    10861 ttaataactt caggctttag accttcacca atcaaggctt gtttgatccc tgatgcgact
    10921 tttcggtatt ctctttttag ttcagtgcta tattcattag aattttcctt gtttgtaaca
    10981 cgagatagtg agctatatct gtttaacgaa tcgaacatct ccgcacctgc atttgttgtc
    11041 gcaaataaaa ttgtatttga caatgaaaca gtgtcgtgaa cttctcgatc atcccatcct
    11101 ctttcagtaa aacttatcct accttcatct aaaattgata aaaaggcatc gagtgtttct
    11161 acaccagctt tctcatattc atcacacaca ataatcgcag aaggatcatg ccttacatga
    11221 gtacctatga gtcgtctaaa acgcattgca tcatcttttg ttttgtagtc cggcatatta
    11281 actaagagaa ctttattttc atcgccataa agtcctcttg ccacggcttt agccattgca
    11341 gttttaccga cccctgttga tcctgtaaac agagcagttg cttttggttg ctttcgacct
    11401 ttaaaacgat caaatccaag cttcatacgt ttaaacaaca ttgttatagt ataaactgct
    11461 aacggttgcc cttttatgga ctttaattcc tcttgaagtt tttccatatc aaaatcagaa
    11521 tctagctgaa tattatactt accaaatatt tgcaatagac tttctcgatt ataagcattt
    11581 ccgtctactc gatcttttgc aatcaaatcc tccattacgg caattgattt agccggttcg
    11641 gacaaatcct cacgataaag cttgtttgct tgtacgattt cttgaagtaa ttcatcagga
    11701 atcctttgcg ataatttaaa agaagcctga ggatcatctt taacagagga tgtatttagc
    11761 cattgtctca aaatatcgac gacaacatcg ctttttagtt cattcaattc taacggttgt
    11821 aaccgtctcc ctaaggcatc atctcttttg atatagtttt catattcttt tcttgtagta
    11881 gcggtaatca attgaagtga ttcatttgct aacggttctt ttagcgaatc acccccaatt
    11941 ttattgccac taccaaaaga gctaacaact ttatgaaact cgtcaatgaa gaggacaaat
    12001 tcattttctt tgtcctcaga ttttaaaatt cgctcatatt ctttcaatct tggaattaat
    12061 gtttctaaac gtgtttggag gacatcatct cccccaacac gcaattgtcc gattgataat
    12121 ttgtatactt gtactttctt tccatcttgt cgctgtcttt cagcccattc acttgcaagt
    12181 gctgttttac ctgttccggc ttcacctaac aagatagctt gcggtgtttc cggtctattc
    12241 aagatcagtg acaactcatg gagttcgtca tcacgtccca ctaacttatg tttcggtggt
    12301 ttcattggtg tgagagcatg attaaattca gtttgaatat tcttaaaaaa ctcggtacta
    12361 ttttccatct gtttgtgttc acttcctcta aaataagaat aatttacttc tagtcagtca
    12421 gagttaggta cttaaatgtc gtcttcatgt ctccaaaaga atctttgtta aagtcagcat
    12481 ttttgtattg atcaatcaat ttttgtgtat cattgattgc tttaatttcg gtctccattc
    12541 gactgtctag ttgacgaata ctttcttgcg aatctttatc gtcaggattt tgttgtaaac
    12601 gttccagata ctcagctttt gtagcttcaa ttttcttgat gttagtattt gaagcattta
    12661 actgatcttc taacttactt acaacattat tcttacttgt aactgctaca acttttgaat
    12721 aatcaatatc gccatctttg gtaagaaagc ttccttgata gacattagtt gaatctgaat
    12781 atggatttat tctgaaattg atattatcag gagtattaac aggttttgct tttttcccaa
    12841 aagaaattac tccttcatta ttaacatcat tcatagaata ttgagaaagt gcttttgtaa
    12901 ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc agctgtatca tcaccagaaa
    12961 ttagtgatac ttggtttgca ataaaaactt gataagctct ttcatctaat ttacctttta
    13021 ggaataagaa agcatcccca tcagtactta acatcccata gctcatttca agctttttag
    13081 gacgatcttt ttctttcgtt gccatatata agttgtagtt ttttccttgc gtagataaca
    13141 actttctagc gttttgcgag taaccgagtt ttactactgt tacatctctg ttttgatctg
    13201 tccacacatc tgaaatagtg atctctgctc ctgatcgagc aaactccaat gaagagccta
    13261 taggtgttgt attgttcacg agataataat tatagtttcc taattgataa atgtagctac
    13321 ccaagcctgt taataaaaaa agcactccta gaaaagttgc aagcataatg cgtaatccgt
    13381 gacgtgttct cttatcctta tcaccaagca accctccaat aatactgtat tgatctttgg
    13441 tagatgttga attgacgttt ttttccatta gtttgtctgc cccctttcat aaaagagcaa
    13501 acaatcagac gttttttcgg taattctagg ttcgggattt tgaatcattt cggtatattc
    13561 ttccccatta atatttaaca caaaaattat cttcctttct tcgttccttt cttttgctct
    13621 ttattttcca aataatcaat gatttttgct attgtaccaa gatagtaatt cccctcggct
    13681 ttgttttttc ggattttgct aaccattgga gtaggtacaa tctcttcatc tctcaattca
    13741 ctgaaagatt ttttacctag atattttcca ataacagatt ttaaaaaacg ttcaaaatct
    13801 ttctcgtaag ttgttttctt gccttttagt tcatggttca tattgtcaac gacttttgcg
    13861 gtttcaataa ttgttttagt tctcagctta ataatttcat ctggaatttt caatcctcgt
    13921 aatttcatcg ttgtactaac tggtaaccca tgctttactg catcagcaat actaaaactt
    13981 tttagatcat ctagtaagct ctccatatag ttaacaaatt catgccaatt aatagcattc
    14041 attgtctcac cccttcattt ttttaaaatg aggttcgggc gtaacacatt tatgttatta
    14101 taatatcata ataacataaa taaatcaatc agcaaattta gatatccctt aaagtatcag
    14161 caattatcca acgagatatt tattttagct atttcagtca tcatgaaaga ctaattagca
    14221 ataaaataac taatattatc catttatcag acgaaaagaa aaaacttatc taattactct
    14281 atattaactt ttaacaaata gttgacttgt ccaaaaatgt taccttcaat ctcacaaaga
    14341 tattaagatg tttcctacca aaactgatat caccggactt tttattctgt gttgtccaaa
    14401 agtgttacct ttagtcgatc aacaaattaa gaaaaattgt ttttttcttg acttgtccaa
    14461 aagtgttacc catagttgtt aaaatttgta tagattagcc attctttcaa atattattta
    14521 gactgaaact tgcttacaaa aaaacttctt gcatttattg tccaaaagtg ttacccatac
    14581 ttttttaaag aagcaggttg tattaaaatt acaacctgct tcttctcaat cttcttctaa
    14641 ccaattatgc attgaaatgg ttggcaagtt tgattctggc tcactaaaag tgaatctgag
    14701 ccgatcaact cttcttcctt tctttgcata gattttttca taatcaaaag tttcaaatac
    14761 ctgtattccg ttttcatcag gagctaaaaa ttcttctttc gcctgctcta gtattctttt
    14821 atcaatattt gtcattctat aacttgaagg tatacttaat gctcttctaa aatcttcaac
    14881 tttgactacc caataaccat ttcgccactc tttatttctg tattgcatga gatgacgata
    14941 caattctttt gtgtagctac ttcttagttt tgtaaaatta tcaagttcaa aacgagtaaa
    15001 gtttttgcca attgagttta agataaattc aaattcctca tttgccttaa ttgtaactgt
    15061 ttccttctgt ttatctatcc ggaatttttg aaaaagccac agctgttcat agacgtctga
    15121 atcttcaaaa cggtaggtta aattacctag tttgtcactc aaatctttta acacagaata
    15181 aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa cgaaagaggt tgaaatcaaa
    15241 agttacttct gctgttcctt gacgtttcat ttttgataaa atagcccaaa acacatccat
    15301 ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga ccattgttat aacgcacaat
    15361 tgcatttgtt tgttctgata ctaaatctac atcactatca ataatctttt cggtaacttc
    15421 ttgagacatt gattactctc caatcttctt tttgcgctcc tttaatcatg ttaatctggt
    15481 actaattaaa ggaaacaatt ttttctgctt gttacctaaa ttaaatcaca actaaaggta
    15541 acagtcaaca aagatttgtt actaatctaa ttttctattt tttcgccttc caaatcaaga
    15601 aaaccgaagg taacgtctta tcctagtgtg atacctcaaa tgtccacttt tgttaccttt
    15661 gctgtccaaa actgttaccc ttactgtcca cttttgttac ctttactgtc caaaagtgtt
    15721 acctttatac ccctttaata cttactctcc caagagatta agcctcccct aacagatata
    15781 ttaacagata tttaacagat atattaacag atatttaata aaggaggatt actcctctct
    15841 tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact aacactatct aggagtttat
    15901 tttgcttgtg taactttgaa taattgaaaa aaaggaaaag gaggaagctt cagcttcccc
    15961 ttttatttat atttataata tatattttaa tctttaaaac tttagcaacc tccaaaaagg
    16021 ctttatatca atcttaaata gattttaggg tggacataaa gcgatgcgaa catggacaaa
    16081 aagcgatgtg aatgtggaca aaaagcgatg caaacgtgga caaaaagcga tgtggtattc
    16141 cgaatatatc caaaaaaaga aaaagtatgt gaatttattt ctaaaaacta gaaaaatatt
    16201 tgttttttaa ttttggtaat ttttataatt tcgtatttta ttgaaaagga tttttatttt
    16261 gaaaatgttc taaaatagcg atttctgctg tattttcaat gaaatttgat tggaaaaaat
    16321 aaaatgtgtt ttactgacat tgagttatgt tataaattta cgatacattt cttattgtaa
    16381 cgtatcgaaa acaggtctat acttattcaa tggaatcgta ccagttccaa gtttttaagc
    16441 tcgtaccaag cttagaaaga agaggcaatc gtatgaaaat aacagaaaag aatgtaaacc
    16501 cgaaatttga aactaccgaa agcaatctaa aaaagtttga tttaactgtt actttatcga
    16561 atcaacttgt agatagcatt gttgaaatgg atgctatgcc acaaaaaatt tatctttacg
    16621 ctataggaca tcttgaccct aaaaatccgc cttttaataa taccgtcaaa gtatctaaaa
    16681 aggctttatt tgcgttgatg tcttctagtt catcttcttc aaaacattca agattaaaac
    16741 aagttattga aaaattgcat cagcaatctg tcattaaaat tcaaagaact aaacctaacg
    16801 gtaagactga atacaaagtc atctcaccgc tagaaattac tgaattcaat gatttttctg
    16861 atgcagtatc tattaaattt agtagttcaa ttatgccttt attaacgaac ttagaagatc
    16921 attttacgca atatacattt gatcaagtag ttaacttgac aagcaagtat gcgatcagtt
    16981 tttttcaatt attcactaaa gattttaatc tgtatgaagc aaatagagat aacttaacat
    17041 ggagtacaaa atcacttgat agattaaaaa atcctgtttt ctccattgca gatttacgaa
    17101 agaaatttag tattgagaaa aaatataaag cttttcgaga ctttgaaaga cgtgtaatta
    17161 aagaaccgat tgcccaactg aataaataca ctgattttaa aattgactat gagcttatca
    17221 agaagggtca tacttataca aacataaagt tcttgcttac acgtaccatg tcagttccgg
    17281 taccttataa gcaagaacaa agagaccctg cttatttaaa aggcgcagaa gataaaacag
    17341 agaaaatgaa tcaatcgtac ttgaaagcta tgaatagcaa atacactaaa ttactaatca
    17401 aaaacttttt gatcagttct gccgacttat gcgatcttaa acttatgagc cgacttcaga
    17461 ataaagttta tccgctttat gatcgtttat ccaaagatag aggatttgaa tcagttgtca
    17521 atcacctatc ttacgttgct actcataagg aggattacac aataaaaaat actgttaaat
    17581 atctcgaaaa atcaattacg agttatttgc cttatgtgaa cctttatgat taacaactga
    17641 aaaaatgcgt gaaggaagga aaaacttttg aaaaaagatg aactgacaaa gtacaatgag
    17701 gagttcttag caacaattga tatgaaaatg gatttaaagt cgtatgaact ttatcaattg
    17761 ctgttcagtt atatctcacc cacaaagcta aagaaaacag gcgtagtaga tttacctgtt
    17821 aaagcgatta ttaattacat ggatgttgat caaaatcttg aaacggaact aaaaaccata
    17881 gttaacactt tgcaaagaca agcagtttat gaaatatcga atctagacaa gaaatataag
    17941 acgtatacta ctgtatctgc tattgatcgg attcattggg atttagaaga gcatagtgta
    18001 tctttcagtt tttcagaaga tgtaattccc ttaattgtct caatcaaaaa gaagtatccg
    18061 ttctttgttt ttaaagactt agcatctttt caaaaaaaat atagcatcgt gttatacaaa
    18121 tatttggcga tgtattataa aaaatatctt atcgccaaaa ataaaggaga ttttgagaaa
    18181 gctgaaatat ataagaatcc cctgattgaa atagttaaaa ttcgtgagat tacgaaaaca
    18241 gaaaatgagt ataaaacaat atcgaatttt gatagacgtg ttttaaggga atcagttgaa
    18301 gaaattaatg aaaaatcaat gctgaagatt gattttgcta aagttagaaa aggcagaaat
    18361 tacaattttg ctgtccagtt taatatttct agtgagtttg atgaaaaacc ttatagagaa
    18421 aaaaaattag gttaatgcaa aatatatctt actcgtttga agatgcttca gacgtatgtt
    18481 aagatatgta gtgccttggg agcaaggtat taatctcctt tagaggttaa ttagcttgtg
    18541 atcgcctatc aattttttgg taggcgattc tttttttgaa accaaaaagc aaaaattatt
    18601 ttttaattag agaggtgcaa tgcgatgaaa tacttttttt gtgcagtatt cgaatggaaa
    18661 ggaaatgaat atgcagttaa attcccagat tttccagact tatatacgca tggtgaaact
    18721 ttagaagatg cttttgagat ggcgcaagaa gtattggaag gatacttggt gcttttagag
    18781 ttgcaagaaa aagaaattcc atcgcctacg cctgttcaag agttaaatgc aagaatcaag
    18841 cgatctgaga atgttaattt atttgtcgta aaagctaata cgaaagttgc taaagctcga
    18901 aacaagcaaa attatgatcg aaaatcagtg actgtggaac actatttaaa tgttttagcg
    18961 gctgaagaga atattaatct gtcgaaagct ttgaatgatt tgttgagaga aaagttgatt
    19021 tgatgtctct cagatgatca tattaagcaa aaacagcttg ttttaggacg tatggattta
    19081 agaaagacaa attatcaaag aatatattaa aagctctcag aagcgttctg agagctttta
    19141 aattgctatg tattatactg catctgctca aattgatttt cttttcatgc ttaccatgta
    19201 tctagttttc catcaagtaa tttttagata tgtattgagg aataaatagc aaatttgctt
    19261 gatataagct cgaattaggt gctttgtaat gtgttaggat aaattataca agaaaatgtc
    19321 aaaagctctc agaacgcttc tgagagcttt ttatcggtag gcttgtttaa cgtgatcttg
    19381 aaagataccc ttttgatgtt tctctcgatc cctgcatcgc taattactaa gtgttcttca
    19441 agtgtgatgt aggtatataa attaagcatc aaaatggtgt agataagcaa aaaactccct
    19501 aaaactaagt gttgattata aaaatctgac ttataaaaaa gaactgagaa tgttagtgtt
    19561 gcttcccaaa aaagaaacaa aattttgtat ttccaaagat agtggatcaa gcgctgtgct
    19621 ttactcatct caaataattt ataataacct gctaattgat cagcaagcgt atcgttaatg
    19681 ggttctgttt gtgtttgttt catctttctc ttctcctgtt tcaattaatc taaaatttca
    19741 gattgagcaa gcaactcatt gtggtagtcg gtttgaaatt caaataacaa tttagccttc
    19801 ttaacgaggt ttaaagcgtt cgttagtgat tcacttaatg cttcaacatc tttaactgat
    19861 tttgcatgtt tcattttttc agtgtgcgta tagcagaatt caggtattga gttaggagtt
    19921 gcctgttcaa taaacatctc aattcgcaag ttattatcct caaaaataat tagatcattg
    19981 gcaaagtgtt tggtactaac gctaatgcca gttgtatctt ttaatagatt attgattaac
    20041 gttcgagaga aatagtttaa gtgtgtagat ttcataggtt cggtctcctt tttacttttc
    20101 aagcaagtct tcttctagtg ctttttgaag taattgagag aagtttatgt ttttctcctt
    20161 ccctaaaaga actaaccatt ccggaacact aacatttttt ctaattgttt ttgtatttaa
    20221 gcttcttcgg aaaagtttaa tgtttagttg gatgattact actacttgat tggctttcaa
    20281 agttaattct tccactgctg atggtttagg aaattctgca tctggtacac tatatagcgc
    20341 taatcctaaa gcatccttag cgtttaaata tgcttcttca agactatttc caaaggtaga
    20401 tatttccggt aagtcaggga aaacgatgtt ataagcttct ccttctttct cgaagattgc
    20461 cggataaaaa atattttttg tcttcatttc ctcgctcctt ttctagtgta agagaggata
    20521 gtatgcctaa taccaaatag gaaggcaagg ctgttaaagc cctgcttgct tccagatatt
    20581 agcttctgtt cctagtggta catcaccatt gtggatcgga acaacgaccg ttcttttagt
    20641 cttggaatta taaaattttg catgactgcc agtttgtcca gtctttttga agccgttaga
    20701 tttgagaatt tttacaattt ctcttgctgt cttaggcata gctaccctcc ctttacaaaa
    20761 tctattatac acattgttgt gtatggaatc aatagaaaaa gaggatgttt tattgcaaaa
    20821 ctaataaaat gatttattgg ttttttcagc aaattgaatg ttattagcgt tgcaaatatt
    20881 cataatttcc caagtatcat tagaagtata ggtatctaag aattcattga cagttacggt
    20941 tgattcgtat ggttcataag tcaaagcgtt ctccaagtct tttgatgaaa tgatcaagac
    21001 tttttcaatt tcctcccctt tttcatttgt gaaaacgtat tccaaagtga tttgttcttc
    21061 atcggtataa ttcatattgt gttctccttt cttgtattaa gaaaaatgga tatttccaat
    21121 tgcatcccac tcggcaccat tgtggtataa gacgtgaccg agcgttacgc ctttgaaatg
    21181 ctcttctaaa tacatctaaa cgcattcaag gctagtacct gctttgagat tgtagaagtc
    21241 tgcatccaat accttttcag aagttatcgg atcaatgcga gttggtacct caataaattc
    21301 agtccaaatt tctgcaaaag tcttaccttc caaagtccac actccctatc aattaattcc
    21361 aaaagcaaat agagaaatca agcgaccgtc aatagtcact tcaacaactg aaaatgccgt
    21421 tgaaacagtt ttaacatcca cactttccaa tttccctatg aaggaatttt tgtgctttag
    21481 aatagctttc tttaactgtc tgttatacgc ttgacgactg ttaaactttt tgaattctcg
    21541 atccttgatc agctgtttaa cgacccctgc attaattgta tagctttctg caatagcaac
    21601 gtgagtaggt tgctgtaatg tagctgttag catattgtca ctccttccta aaagttaatt
    21661 agcttgttta caattacatt atacacacta gtgtgtataa tgtaaagaga ggttttttgc
    21721 tgatggatca gctgtgctaa acaaaagaaa acgatagagt aacaaaaaaa gtcagcaaag
    21781 ttaaaaagga ggaaacggct agtccggttg ctttgcttgt gtaagtttcg gctttcttat
    21841 agatcaaata ggaaattatc agtgatattc cagtaaagaa taatgtacta gctgtcacta
    21901 aaagaaatag gttaatcata cgatacactc cttctgtaat attggtatca gatacgttta
    21961 aatgcgtttc tgagctgttt tggatcaatg gatgttttag ccctacccta aaatcaaagc
    22021 ccagaaaagc atttaaaaac gttcgtacct aatacgttta tattcatata catatacata
    22081 aacataaaca taaacgtata cacgtttatg tttatgtatt taagttttag gctacttcct
    22141 taacatctcc tgctgatagt cgagtgaacc gagtgagtga tccatcttgt agtctaaaaa
    22201 taaagctacg tttgagttta tactgataaa cagcgacata cgatccatcc cagattgagt
    22261 aagttttacc tgcaactaat aagtcatcaa ggtttggttt attgttttct tctttaattc
    22321 tatctagttg ttgcttaacc attttttctt gatcaccgac aaagaatttc aaccattttt
    22381 tcggaatttt cttaaagtaa gcaggactgt catttaagaa gtagattggt ttgtacattt
    22441 catctcgaaa gatgaagctg tcaatatcgt aggttccttc tgcatctttg aaaaggataa
    22501 taccataggt cttcgttact ttggtatcta cgatttccaa accatttttg gcgtaccctt
    22561 tcttttcttc gatcacttca tttgctgatg aataagcacc ataccatccc ataaaaaatc
    22621 gctccttttg aaagttaatt agcttgttta cacttatatt atacacacca gtgtgtataa
    22681 taacaagagg gtattgttct tttttaggta aagtgggatc gtaaacagtg attttgttgg
    22741 gttaacaaaa aaacagacaa gccttatcag cttgtctgtt acttataaac ttcacttctg
    22801 tgaccaatag acaatataag aattgtcact tcatgctcgc taatatcagc tattatacgg
    22861 taggcaccta ccctatatcg ccagtagcct gatttattag ctgttagacc tttaccatgt
    22921 tttcttggat catcagtgcc tacaagattt ttctcgatcc agtttaggat aacctttgcc
    22981 tgaaacttat ccattttttt tagcgctttt tgagcttcac gttcatatct tacttggtaa
    23041 gtcatcttat aagcctaact cttttagcat atcttcgtgg gaaatacttt gatcgttttc
    23101 ctcatgctct ttcattagtt ccttgtaggt ggcaaagtct actaacgttt ctgctgtttg
    23161 cagggaatta ctacgcataa attcagaaat gttatctccg ttgaattcag caactttttc
    23221 aagaaaatct ttttcttctt tagatacacg aatacttatt actggcatag tttatcaact
    23281 ccttttcaat tacattgtac tacgattgta tgacaaatgc aatagttata tatatgaata
    23341 tatacataaa cataaacata tacatatata tgtttatgtt gtagaatata aaagcaaata
    23401 taaatataaa cacgtttatg tatataaaca tatacataaa cgtgtttata gtgtataatg
    23461 gagttacaaa aggttaataa gggaggttta atcatggaaa agaagttgtc aatcattact
    23521 ggaaacattg aaaaaggtgg aacaggtaaa acaacaacaa tctacaatct aggtggatat
    23581 gctgtcgaaa atttaggtat gaaggtgctg tatattgatg aagataagtc gcaaaaccta
    23641 tcaaaacgat ttcctgatgc tttcagtaaa gcgaaaagag agaatacaat caatcattta
    23701 tatcttcatg gatcagctga acctttagct gtaaatgaga acatggatat tttagtagca
    23761 ggtgaagatt taaaacaagt tgagatgcaa atgcgtgata tgcctaacaa cagaacccta
    23821 ctgtttagat ggatggttgt taactatgaa cagcttaaag agaagtatga tctaattctg
    23881 attgatactc ataatgatgc aggtctacta gctcaaaatg cttgggctgt atctgatctt
    23941 gtcgtaggca tttctgatcc atctaaagat ggttttgaag ctttgctgaa gcttggtaaa
    24001 gatattgatg cactagcaaa acagttgatt gatattaaga ctggtgaaac actgatggaa
    24061 actaaatacg ctacgatcgg tagcaggatc aaacataata ctaattcaag tcgtgagttc
    24121 ctagaactgg taccaacgtt atctaattat cttggatatg tacaagagaa ggaattgctt
    24181 aatgcaacta gcttagaagg taagaatatt gctgagttcg ctaaggataa gaatatctat
    24241 cgtaagaata aggatttctt tgaaagcacg tttacgctat acaacaaaat aattgatacg
    24301 gcacaagaat aaggagtgat tgaagtgaaa gaaaatagat ttaaaaaatt cgctgaagct
    24361 gaagtagaag aagttcctga agtggttgaa gcagttgaag aacctaaaca aacacctaaa
    24421 gaaacaacaa gttctttggt agctaaacta aaaagtgata gttctgaaaa gaagaaaaat
    24481 tatcaattca cgcttcaacc atcgcaaaga aagaaactat ctaaaatcag cgaaaagtta
    24541 ggaattcgat cagattcagc tttacttggt gaaatgatag ataacttgta tgatgaagtt
    24601 tttggtgagt gatcagttta aagctgatca ctttttttat tggaataagc ggttaggtat
    24661 agcttaacta aatttaatat acgtgtttat ggatatatac gtatacatgt ttatatacat
    24721 gtatacgtat atgtttatgt ttatatttgt acataataaa aaggctaggt attgtaacct
    24781 cgccttttta tttttaaagt aagtctttgt tacttggtgc attgaaccct ttcagcattg
    24841 gcttttcttt ctttgctgaa tgatcggatg taaccgttga aggatgctct tgttgaatag
    24901 ggttatgatc agttggttta ttctttactt ctgctacttt gctatcgttt ctatcgttgc
    24961 ctgcttcaat aattgtaact tcattgttct ttacacgccg atataggttc aaagcttttt
    25021 tgatcgtctg agagtattta acgcctagtg tttcatattc ggattcaaga aactctgcta
    25081 aaggtaactc gctaaccttt tctctattga ttataactac ataacgctct gattttccct
    25141 tattcatgtt gatctccttc gctgttttat tgagttaaac caaacttgta aaagcctttt
    25201 acattagaaa attcactgtc ttcaacaaac aaggtatgcg gaattttatc ttttacaagt
    25261 tgtttgtcga taatagaagc tccaccacca gtaaagacaa ctttatctaa ggttgtgaag
    25321 tttttgaatg tagctaaaac ttctgcaaca accatattgg tgtatgtttt tatttctctt
    25381 tcaactactt ctgaaatatc aaaactttca tttccgcttg gcttaaatag atatacgcct
    25441 ttttctttgc cttctcggat aatcttttca agttggaaag cattaggact gatcggaagg
    25501 tcaagcatat ttacgatacg attatataaa gtaaaaatgc cggtatcctt ttgttcacga
    25561 ttgttttcgt caatttcaaa attttggaca acatcaaaaa gtttcgtgcc accgccaata
    25621 tcaacaaaac cgatcttctg actgatcaat gtttcatctt gtacactacc atcatcagca
    25681 agtaggaggt tatatagtgt tccaatcggc tgtggcagta cccaaacggt atctacgttt
    25741 atattataag ctttaccgtc aacttcaact gaatgttgtc gtgtggcttg ttgcatcacg
    25801 tatttgacga ttgtttcggt atagtcggat gaaggcagac ctaaaactag attagcggtt
    25861 aaaacgtcat cagattgatc ttctaaacgt gctaggttag ctaaagagaa agataggata
    25921 ttttggtagg tttcctgctt gtatctgctg atcccaaaac ctagtgattc tatcacgtag
    25981 tcatcttttc ctagctcgtg tatatccttc ccaaagtaat agctttcagt agtgtttact
    26041 aggctatatt cagaaacgtt tagtgatcgt tttaatgagg aacctatatt agaaaaacgt
    26101 cctgaattaa tgatattttt gttcaaaata cgagagggaa gtgagagtgg agaaacgtgt
    26161 gtactcattt cttttactga tttattccct aagtcaacgg aaaacaataa tggttctttc
    26221 atggtatgac ctctttctgt ttgtgtttat ggtatttcgg tacgaaaata gtttacacct
    26281 atttactatt agttgcaact gtttataaga aaaaaatgca atcataaaag tctaattaat
    26341 tataataaag tgtaaataaa aaggtgtaaa taaatgcttg caaatttatc ttattatgat
    26401 attataaaaa cataaattat attaaggagt gatcctgatg cgattgagaa gaaataagaa
    26461 ttacactacc attattttag aagattttac tgatcaagaa ttacttattg tcggtaaaac
    26521 aaaagcagat tttatcgaaa agggaaatgc ttcaatagta aaagcttcaa agattgctgt
    26581 cgaaaaatat tttgattctt tagaagaaag tgctaaggaa atttttaatg gagagaaagc
    26641 tggtagctat gagttagacg gtattagatt tagcttgatc ccaacaactg aaaatattat
    26701 tccgaagaaa ttatattata tcttttcatc tgaaaaacat acttcaatga agccttgtat
    26761 ttttgattgg caatggttga ttgatgctgt attggatgta aaatgttcaa aatacaatat
    26821 tactttgggg tgttaattat gagtatgcta gttagaaaat taccattatc tgtaagagaa
    26881 tttctatttt acttggtctg cttaatcgca tcagggttta agctgatcga acatattttt
    26941 atctttgctt atgtattagc aatgatcgca tttttatctg gcggttggtt aattatttat
    27001 ttgttaagct aagttttaga tcaaaaataa ttaataaatt aaacaataaa ataaccgtta
    27061 ataaatgtaa tgaaaggaca acgcctaaca atcagcttgt aataagatta taaaataaca
    27121 ttatattaaa ataagtgctt tttaaaggac aaattaagtg aatcaacata aacgtgtata
    27181 tgtatatgtt tatgttgata cttttttgtc ttaatttttt tatttttaaa attctattta
    27241 ctaatgctgt atagatagtg atcagaaaaa acaaaatagc gaggttaagc tatttattag
    27301 ttcatatatt atattcagat taatatgatc cagtattgat ctgaaggcaa aaccaatata
    27361 tttctacgga ttcttgtaaa actatttaaa ttttcctgct aataaaataa caaaaataga
    27421 atatttagct tttttatacc attttacaat aacatgattt tatttgtaat attaaaacat
    27481 ttaaattttg attttttttc aaattagatc aattaatatc cttataaaag agcataataa
    27541 atgattttta catcgttatt tattgatatt aatctcatcc aaaaaaatga aagcgcatac
    27601 tttatgggtg attaaatgtg attttaatac atataccaat taaaagaaaa cttttttaga
    27661 tttttactta caaatttata aaaaaacaag ataattgata ctttgatttt gcgaacattt
    27721 tatctttccg cctaattcag ctgcaatatc atgattatat cagtaacaaa tcgctttttt
    27781 agctaattta agcatcaaat aattattctt aatcagtgaa aaaaaataac gaataaatgg
    27841 attttgggaa taggtaaaaa cttgatcggt ttctgga
//
LOCUS       DACDCJ010000034        27711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDCJ010000034 DACDCJ010000000
VERSION     DACDCJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.034"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..107)
                     /locus_tag="IV038_001414"
     rRNA            complement(<1..107)
                     /locus_tag="IV038_001414"
                     /product="16S ribosomal RNA"
     gene            26..169
                     /locus_tag="IV038_001415"
     CDS             26..169
                     /locus_tag="IV038_001415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341643.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(787..2283)
                     /gene="lysS"
                     /locus_tag="IV038_001416"
     CDS             complement(787..2283)
                     /gene="lysS"
                     /locus_tag="IV038_001416"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP8341644.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2394..3398)
                     /gene="dusB"
                     /locus_tag="IV038_001417"
     CDS             complement(2394..3398)
                     /gene="dusB"
                     /locus_tag="IV038_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP8341645.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3399..4286)
                     /gene="hslO"
                     /locus_tag="IV038_001418"
     CDS             complement(3399..4286)
                     /gene="hslO"
                     /locus_tag="IV038_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP8341646.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4503..6614)
                     /gene="hflB"
                     /locus_tag="IV038_001419"
     CDS             complement(4503..6614)
                     /gene="hflB"
                     /locus_tag="IV038_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP8341647.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6704..7249)
                     /gene="hpt"
                     /locus_tag="IV038_001420"
     CDS             complement(6704..7249)
                     /gene="hpt"
                     /locus_tag="IV038_001420"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP8341648.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7249..8694)
                     /gene="tilS"
                     /locus_tag="IV038_001421"
     CDS             complement(7249..8694)
                     /gene="tilS"
                     /locus_tag="IV038_001421"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP8341649.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8814..9284)
                     /locus_tag="IV038_001422"
     CDS             complement(8814..9284)
                     /locus_tag="IV038_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP8341650.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9330..9755)
                     /locus_tag="IV038_001423"
     CDS             complement(9330..9755)
                     /locus_tag="IV038_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP8341651.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9822..10091)
                     /locus_tag="IV038_001424"
     CDS             complement(9822..10091)
                     /locus_tag="IV038_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP8341652.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(10102..11697)
                     /locus_tag="IV038_001425"
     CDS             complement(10102..11697)
                     /locus_tag="IV038_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8341653.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11712..15233)
                     /gene="mfd"
                     /locus_tag="IV038_001426"
     CDS             complement(11712..15233)
                     /gene="mfd"
                     /locus_tag="IV038_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP8341654.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15250..15810)
                     /locus_tag="IV038_001427"
     CDS             complement(15250..15810)
                     /locus_tag="IV038_001427"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP8341655.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            16163..17137
                     /locus_tag="IV038_001428"
     CDS             16163..17137
                     /locus_tag="IV038_001428"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP8341656.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17307..18707
                     /locus_tag="IV038_001429"
     CDS             17307..18707
                     /locus_tag="IV038_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP8341657.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18785..19507)
                     /locus_tag="IV038_001430"
     CDS             complement(18785..19507)
                     /locus_tag="IV038_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8341658.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19586..20092)
                     /locus_tag="IV038_001431"
     CDS             complement(19586..20092)
                     /locus_tag="IV038_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP8341659.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20215..22014
                     /locus_tag="IV038_001432"
     CDS             20215..22014
                     /locus_tag="IV038_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP8341660.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(22082..23620)
                     /locus_tag="IV038_001433"
     CDS             complement(22082..23620)
                     /locus_tag="IV038_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341661.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     STTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
     gene            complement(23875..24384)
                     /gene="mreD"
                     /locus_tag="IV038_001434"
     CDS             complement(23875..24384)
                     /gene="mreD"
                     /locus_tag="IV038_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP8341662.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24388..25242)
                     /gene="mreC"
                     /locus_tag="IV038_001435"
     CDS             complement(24388..25242)
                     /gene="mreC"
                     /locus_tag="IV038_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP8341663.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25397..26035)
                     /locus_tag="IV038_001436"
     CDS             complement(25397..26035)
                     /locus_tag="IV038_001436"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP8341664.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(26036..26686)
                     /locus_tag="IV038_001437"
     CDS             complement(26036..26686)
                     /locus_tag="IV038_001437"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8341665.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26699..27598)
                     /gene="rsgA"
                     /locus_tag="IV038_001438"
     CDS             complement(26699..27598)
                     /gene="rsgA"
                     /locus_tag="IV038_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP8341666.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
BASE COUNT         7613 a         5979 c         5039 g         9080 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttgtt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcgagag tgtttcccga ttcatttttg gctgccgtag caacttttat
      301 atcatatcag agagacaagc gattgtcaac tcttttttaa aagttttttt cgatttcgtt
      361 tttcgaaatc tttcggcaac tcatttatca taacttatcg tttgttattt gtcaagaact
      421 tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag
      481 ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt ttgaataaat
      541 attttcttct gtcttttgga caagtaataa tataccaact aaatttagct tggtcaacga
      601 attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt ttctttcttt
      661 atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt tgaactttcg
      721 ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta gacagctttt
      781 attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg catcagttag
      841 caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca taccgtattc
      901 taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta gttcacgttc
      961 tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt ctgtaaaggc
     1021 attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc gaggatcttc
     1081 tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa acgtcggttg
     1141 tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc cgacacccat
     1201 agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt ccaatgtcat
     1261 ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat ggatgcgttt
     1321 aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg ttcctagtac
     1381 tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat tgaaatcagt
     1441 atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat caattccttc
     1501 attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa ttagtcgttt
     1561 taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat tatgatgcgt
     1621 gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg tttctacttc
     1681 aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc ttcgtttcat
     1741 aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc tgtagcgttg
     1801 ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt ttgacaaaat
     1861 cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga tttgtccagt
     1921 tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct catcacctac
     1981 ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat gcgcaaatcc
     2041 agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg tcaatcccat
     2101 ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt ctgagttgtg
     2161 cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt gttccatttt
     2221 ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt gttcctgtgt
     2281 cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg aaacacaaaa
     2341 aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag aaatcacaac
     2401 gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag gtcattgatt
     2461 tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg aggaatccct
     2521 tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc tcctttgaga
     2581 tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg tgttggttca
     2641 ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt gaatcatcca tggattgccc
     2701 agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat ccattttgca
     2761 tcttctggcg ttttgacatc cccgttcccc ataaatggga tcgtcaaatg ttttttcact
     2821 tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt acgtccatgc
     2881 atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac agcaaagacg
     2941 tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac tgctgaagaa
     3001 acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc tcctgcttct
     3061 gctttgatta ctttatttac tggacagccc atattgatat ctattatatc ggcagctgtg
     3121 ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc aaatatctgt
     3181 aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt tttgtttctg
     3241 aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc aaattctttg
     3301 acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac gacacgatta
     3361 gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc taaggcttct
     3421 ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca gaaagaacaa
     3481 accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc acctaacgcg
     3541 ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac aggcattttt
     3601 tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc tggtgtctgt
     3661 ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc gatcttgctg
     3721 attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc cgtcttgatg
     3781 ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg ttctgagata
     3841 gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt ttgcccagaa
     3901 aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt tccaactgct
     3961 ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg atttttgatg
     4021 taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc tggaccatcg
     4081 ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag tagcagacta
     4141 ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg acgtctttgt
     4201 gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc gtcgtgagca
     4261 agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt tttaaactca
     4321 tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct tttaaaaata
     4381 attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata aagaaatctc
     4441 ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg acctatattt
     4501 ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc tgaatcatgt
     4561 tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg tttttcttcc
     4621 gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa agttttcgct
     4681 tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc gtgttcaaac
     4741 aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc agcaatcagt
     4801 ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc ttccatcaag
     4861 atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc ttttgtttga
     4921 ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg acctaaacga
     4981 tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg ctcaaagtca
     5041 ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg tccaccaagt
     5101 aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc ttcttttggt
     5161 aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt gtggacgacc
     5221 cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata agcaaccatt
     5281 tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat cacgcgatct
     5341 tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc agctactaac
     5401 gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt ctgttgagcc
     5461 acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac tcgcaagatt
     5521 gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa acggcctgga
     5581 cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac gattactcct
     5641 tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg ttcgcgttca
     5701 tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat ttcatcaatg
     5761 aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac acgacttgct
     5821 ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa cggtactcca
     5881 gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg tccttctaaa
     5941 agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc tttcagaaat
     6001 tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc agaaaaacgt
     6061 acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt catgacacgg
     6121 ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa aaagatgatg
     6181 attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct tgattcttct
     6241 tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct caatgttgta
     6301 tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga acctaagata
     6361 gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt gatcttatat
     6421 acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag ttgttctgtg
     6481 aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat gaaataaacg
     6541 accattacca tcgctaagat tacgaggaca taatacaagg catttttcat catgccgttg
     6601 ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt tttttatcaa
     6661 tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt gattgataaa
     6721 cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca taatccaagc
     6781 cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct tcgatattta
     6841 ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt gctttgcggt
     6901 atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata tcttcaacga
     6961 tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt acttcccctg
     7021 acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc aaatacgtat
     7081 cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca cctactacta
     7141 ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg caacgaactt
     7201 gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc aggttctcct
     7261 tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat cagtttcttc
     7321 acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc ttttggcatc
     7381 tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga taaaatatct
     7441 agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt ttcttttttc
     7501 gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag accatgaatc
     7561 tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt ctgtttcatt
     7621 caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct ggctagttgc
     7681 ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta tccaattatt
     7741 gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa tcgactccat
     7801 ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa aggagtaaaa
     7861 aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa atcgaaattg
     7921 ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt gaatgacttg
     7981 gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct gtgtattttc
     8041 ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat ctaacaattg
     8101 gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat ataggtcttc
     8161 tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt gtacttcttt
     8221 gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca ccgtctcgat
     8281 ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt ccatcacgcg
     8341 agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt tggctggatc
     8401 ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc gaagataggc
     8461 agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac ctaaagaaaa
     8521 accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat caacaccacc
     8581 tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat tcccgtgccg
     8641 aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt tcatcctttc
     8701 cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac aattaacctg
     8761 ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc tttttagctg
     8821 cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga agataagcgg
     8881 tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt ttgagctgaa
     8941 ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc acggggatct
     9001 ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg ttcctgcgct
     9061 ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt aactgttact
     9121 tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga cacttcgcta
     9181 atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa atttgtgatt
     9241 cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa aatgttcctc
     9301 ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact agttgattga
     9361 tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac tttttcaccg
     9421 tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc atctttcagc
     9481 aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat ttcagtattt
     9541 gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa aatctgcaca
     9601 cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct gcgtcttcga
     9661 aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc atattccgta
     9721 tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa tctttttgat
     9781 tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc tagtgtttct
     9841 gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt ggttgattca
     9901 tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt caaaatatcg
     9961 cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat tcttccttta
    10021 tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt taaaaattta
    10081 tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca ggattttctt
    10141 accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa tcactgcata
    10201 aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag cagtggaacg
    10261 atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca gtactactgt
    10321 catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc aaaaagcatt
    10381 tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc ctaaaagggt
    10441 agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag tcaatccttt
    10501 taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct tgctttgagc
    10561 aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa ataatacaag
    10621 cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga gcagcaaggc
    10681 taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga ttttggctgt
    10741 ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag gcaggaatgt
    10801 cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac ctcgatcata
    10861 gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca aagcattttt
    10921 tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa tcgaaaccag
    10981 tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga acaatggaaa
    11041 ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa ggatcccata
    11101 tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt ggtacaccgt
    11161 ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac gaacgaactg
    11221 ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga aattccctcg
    11281 ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga tcagcggttt
    11341 caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga caaacaacca
    11401 caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt gctttcgctc
    11461 gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac cggataaagc
    11521 taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa aaccttcgtc
    11581 gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg ctatgaaaga
    11641 agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat gatccaaatc
    11701 attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac aaacttttgg
    11761 atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg tatcaccatc
    11821 tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc ttcgaataac
    11881 tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat atgcgcctcc
    11941 tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg cccaattgtc
    12001 aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc atcttctagt
    12061 tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc gattttctgc
    12121 cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc gatctcaaca
    12181 gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc gcttagcatt
    12241 tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc acctaagaga
    12301 ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga tcccagttct
    12361 gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat cttctgctgc
    12421 tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc tcgcaattgg
    12481 tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt tgcgttcggg
    12541 atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg accttcaata
    12601 aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc ataaccgatc
    12661 cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt ttctacccga
    12721 ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc ttcgcgaata
    12781 gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc aggcggtgtt
    12841 tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct cggaatcggt
    12901 gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg ttccttatgt
    12961 tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct aaattcaata
    13021 tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt acgcagttgt
    13081 tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc gatatttacg
    13141 gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa gatcgttgtt
    13201 ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc tcgaagcgct
    13261 acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg tttttctttt
    13321 tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc agagtagggg
    13381 aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata tcctttttct
    13441 gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat ttttgaagag
    13501 acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg aggcgctttg
    13561 gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat aaacagtttg
    13621 tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac ttctagcgtt
    13681 tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc tcctgctttt
    13741 aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct cgtttgtttt
    13801 ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc ttgaggaagt
    13861 tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat caatgcattt
    13921 ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc tttgcgtatt
    13981 ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca acggtccatt
    14041 tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata ttgaaaatta
    14101 tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa aaaggttgtg
    14161 gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg ctcagaaaac
    14221 acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc gatttcccga
    14281 tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga agggaaataa
    14341 tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc ttcttctgtc
    14401 gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa aaactcgcgg
    14461 tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat ttgctctatt
    14521 ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac ccagatttct
    14581 tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga atctatctct
    14641 acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat atcgataatg
    14701 cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac atagcccatc
    14761 aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg ccaattcaat
    14821 tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc gactggcaac
    14881 aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct ctcgcctcgc
    14941 gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg aaataaatgg
    15001 atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc ataatacaaa
    15061 tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc aaatagaagt
    15121 gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc ttctaaattg
    15181 ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc cattcctttt
    15241 cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt ctatgagata
    15301 ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat tttctttgct
    15361 gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc cgattcccac
    15421 cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga taccgttatg
    15481 cccgccggca cttccctttt gacggagccg gatttttccc actgccagat ccaaatcatc
    15541 gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg ggccgactgc
    15601 acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt ctccattatg
    15661 aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt gatgctcgcg
    15721 tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt atttggtacc
    15781 tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc taaaaagcag
    15841 cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc ttttttatac
    15901 ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa tcgttaataa
    15961 atgataaata aattaaaaat ttcttcaaag tttaatgaaa taattgatat ataaaaatag
    16021 tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg ttatatttta
    16081 tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg aaaaaacaaa
    16141 tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc atcaaaaagt
    16201 tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag ttactcaaaa
    16261 tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag gagacgcaat
    16321 tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg ctacttacgc
    16381 agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa aaccaggtga
    16441 aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg tcacacaaat
    16501 cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag atattttgac
    16561 ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt caggtacctc
    16621 tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg atgcacgtaa
    16681 cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt ggtcacatgc
    16741 aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa tcgatgaaga
    16801 agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta tcgagaaaaa
    16861 aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg ccatcttaga
    16921 tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg gattgaacga
    16981 catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag taatcgaaat
    17041 tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat taaaagaaat
    17101 tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga cggaaaacat
    17161 ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa ttttttcata
    17221 aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc gttccaccct
    17281 aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa aaatcgaaac
    17341 atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt tatacctatc
    17401 gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga ataaatgtca
    17461 gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct gataaaaaac
    17521 tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag ctcggctaca
    17581 aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga tggacttggg
    17641 gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt caaaaccacc
    17701 aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg aacaaagacc
    17761 ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt atcaagcctg
    17821 aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa agtgcggtaa
    17881 actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa gtaacatctg
    17941 aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat gtaaaagcta
    18001 cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg agttggctga
    18061 cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat gtaacagatt
    18121 taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca aaaagaggag
    18181 aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt gagacagaag
    18241 cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt gtgcaaggtg
    18301 gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat ctggagaacc
    18361 agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc gtttccggaa
    18421 agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa gaagatgcaa
    18481 ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac tactggatgc
    18541 cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa tacggtggag
    18601 acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt gtaatgaagg
    18661 aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa aaataatata
    18721 taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc taacaatgtg
    18781 ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct tctagcttat
    18841 ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc gtttgtggaa
    18901 tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct cgtacagcag
    18961 caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc agatcttctg
    19021 cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac ccatcgcgtt
    19081 caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt tctcccagtg
    19141 atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta cggttatctg
    19201 tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac gtgatttcat
    19261 cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc acattgtcgt
    19321 taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga ttcatagatg
    19381 gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc tgaaagactt
    19441 tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat ttactatgtc
    19501 ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta gtactatttc
    19561 acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca aaaagcacac
    19621 ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg aacataggtg
    19681 ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct gttgctgaaa
    19741 gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca cctaaaaaca
    19801 agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa aagaactcta
    19861 aaaatttggc gtagcccaga tggtcgatac tcaccatact tccaccataa ttaaaacgta
    19921 tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat cgcgaaactt
    19981 gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg actatcagtg
    20041 cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc ataagtcacc
    20101 tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata gagacttctt
    20161 ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt tttcatgagt
    20221 tattcattaa attggaatct agattctatt ttttccggag gcagtcattc tgatgctttg
    20281 aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg cgtaacaaaa
    20341 tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt gcaagaaacg
    20401 attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc tgccaatgtc
    20461 aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc tcgtctgcaa
    20521 tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga ttggaatcag
    20581 atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat tcgtcgtgat
    20641 ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc tttagatggt
    20701 ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat tcccttcgaa
    20761 caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat gatgggcgat
    20821 ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg gaaagaaaag
    20881 gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc tgactatgaa
    20941 cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt gaagaaagaa
    21001 acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt taattacttg
    21061 actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca agatgcacct
    21121 attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc agcatttatt
    21181 atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc tgcttttgaa
    21241 aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta ttgtactgag
    21301 ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt taatgaggtt
    21361 gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg ggatttacct
    21421 tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt cgcagaactg
    21481 atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat caatttgcta
    21541 gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg attcattttt
    21601 gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa gatcacagag
    21661 atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta ccatccatat
    21721 ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa cttcccatac
    21781 acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa gggagcaaat
    21841 tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac ggaagatctt
    21901 gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc aggtatcgac
    21961 atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat ctgatttcta
    22021 ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg attaatcatt
    22081 cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact tttacgcttt
    22141 cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct acgtggtaag
    22201 ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa actgaaattt
    22261 ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca gttacttgca
    22321 tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta ccgccccaaa
    22381 catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca ttcacacttc
    22441 cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt gttggcgttg
    22501 gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct gttgatggtg
    22561 ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga ttcaggcact gttgttgatt
    22621 cttctgttgc tgaagattca ggcactgttg ttgattcttc tgttgctgaa gattcaggcg
    22681 ctgttgattc ttctgttgaa gattcaggtg ttgttgattc ttctgttgct gaagattgtg
    22741 tagctgatga tgattcttca gtcattgaag attgtgtagc tgatgattct tctgttgctg
    22801 agctttgtgc agtagatgaa gcttgagtag cttgtgcttc agcctctgct tgttcgcgtg
    22861 cttctttttc agctttttct tgtgcagctt gttgacgagc ttgttctgct agacgttgtt
    22921 gttcacgaat acgagcttgc tctgcttcag cttctgcttt ttgacggttc aagtcagctt
    22981 tcttgtcttc agcagttgct tgttcagcag ctaatgaagt tttcagtacg ttcaaatcag
    23041 cttgtttaga taaaagatcg cctttttgag attccaaagc agcttggttt tcagctaatt
    23101 cttttagttt agcatcgttt tcagcttttt tgtcttctac agctttttta tcttgttttt
    23161 gttgttccat caagtcgttg tttgctttta ccatcgttgt cattgcttga acgcgtccaa
    23221 tagcatctgc taatgaatca gcattcaata cagcatcgat gtagtttgag cttgtgttgc
    23281 ttacttgtgc ttcgcgtgct tgtttttgga ttgtatcttc acgtttttca atacgttctt
    23341 gtaaatctgc aatgtctttt actaattgtg cagattcttg acgcaatgta tcttgttttg
    23401 ctaataggtc ttgtgcttgt gtgttgattt cagaaacttg gctttctaag gcatcgattt
    23461 gtgattgtgc atctgcttgt tgatttttca agtctgcaat cttttgatct tgctgttgta
    23521 tctgagaatc aaaatcgtcc gccgcggcag cgatcggtga tgctacggca gtcaaggtca
    23581 ttgaacatac cattactgct gatattaaac tctttttcac tcttcattcc tccgactggc
    23641 ttaatttaat aaatatctaa atatatttaa gtctttctta acgatataaa aattgtacca
    23701 tagcagtgtg tcaacgatat aatagttatg ttacaaaaat gtttcaaaac aacttttaaa
    23761 aatgacaaaa ctgttcgttt cagaaaatga tcatgaaaaa gccgcgttaa gtctagcatt
    23821 tcttaagata acaacgattc tttttcgctt tcgacctaaa aataaaaaaa gaaattattc
    23881 atcactgaat aatttcttaa agggaaaaat gaataataca aatataatca tgttcaacag
    23941 cagtgtaggt cctaaaaacc ttgtaataaa atacgtgtta tttactactg ctaatttaaa
    24001 aatcaactgg accaccatcg taaacaattc ataacccgtg acaaagatga tcatactgaa
    24061 aaattcagta aagatgttga catggataac gtctttcatt acatacatca accatacaat
    24121 taaagggaga gctactgcat agataccgat aacgccaata tagtaagcgt catagatagc
    24181 cccgagaaca atcgtggtaa tcagtaaata acgtttttca aacgccatac tgcaacataa
    24241 taatgccaat atcagaaagt gggcatttga catataggtg cctttcgacc actcccctag
    24301 cattcttgtt aaatgaccat ctatcaacat caaaaagaac aaaacgattg gcagatagta
    24361 cttcattgtt tctttcttca acatggacta atcctccgct agccgttgga caatcgtcac
    24421 aactgacacg tcatacattt ctgcataagg tttcacataa acttcccgat ccagcccata
    24481 actatctggt ttcgttttaa taaccgtacc gatcggcaag ttagctggag aattccctcc
    24541 aagaccggat gtctggacaa catccccttc tttgatatcc gtatcaccag ttaattgggt
    24601 caccactaag gcatggagct tttcatcata gtttttaagc aaaccaaacg cttcgccatt
    24661 agccgaagat acccgtactg gaaaatgatt ggagctttca ttagaggatg acagtaattc
    24721 gattttagac gaagccgtat tgacctcgat cactcggcca atcaatcctt tttgcgacat
    24781 gactgccata tttgcttcga taccgtcttt agaacccttg tctacgatca gcatgtcctg
    24841 ccaactgtca ggcgaacgag taatgacatt tgccgttact ttttcgtaac tagtcagcgt
    24901 ctgattcaac tcaagttctt ttttcaacgc ttcgatttct ctctcgtaat tcttgttttg
    24961 ctgtgaaacg tcatcatagc tatcgatttt ttcttttagc cgctcatttt cggcataagt
    25021 tgaaaaaaga ttatggacag aagctactcc atttcgtacc caccgtactg gggcagagat
    25081 tgttttatca accattgcaa cactgtcatt aacgatggat tggaagaaat tgggctttcc
    25141 ttcattcgct cgctgcgcag cggtcacact tagtatcgtc accacgatga taacaataac
    25201 caaggtaata ataatatttt tattaggatt gaacttcttc acaaagacac ctcgaattaa
    25261 ttagactctt gtgttcaatt ttatcactaa atgccgtgaa acaaaaggta agttgctcct
    25321 cttaagcttt tttttagcaa ataatagatt ctgtcatttt tagcctgaaa gacaaatttt
    25381 tttatttgtt tatggatcaa tctttgcttt gaataactgc aatcatacca tctgaaaaag
    25441 aaaattctgc ggtatctgaa agaaattcgt tacttgcata agatgtcgga tattctactt
    25501 ctttattttc caatacatac ttgctgcctt ttaatgtaag attcgagacg ggagtcaagc
    25561 agcaataagc taaatacttc atatctggta tctgtcttac tatatgctct ccaggcaaat
    25621 aataagacaa ataattttga caatctctga taataagctg atgggcaaag ggtttgaagc
    25681 gaggttccat acctaaccag agattggcta aaagatgatc caatcttccg cccgtagctc
    25741 caatcaaagt gacttccgct tgcgggtaac gttcaaatgt ttttaagata cctaactgtg
    25801 tatctgtatc gtctttttct gcaggggaac gggtatactc tttggctttt gcaaatactt
    25861 ccttctgctc ttcgttcgac aatgaatcaa aatcacccac tgccagatct actgggtagc
    25921 ctgcttgaag caggtacagc gcaccacggt ctatcccgac ataggcatcg tatttggaca
    25981 gcagagactg cggccagttt tgcggattac ctccagcagc taataaaatt ttcatttagt
    26041 ctaatgcctc ttttaatcgt tcgatttgtc cgactggatt ttcggctcca tagatatatg
    26101 atcctgcaac gaagacatcc gcccctgcat ctttacagat ttttgctgtt tctggaacga
    26161 ttccgccatc tacttcgatt gcataagtcc aattgttctc tctacgtaga tcagcgagtt
    26221 ccgtaatttt ttctactgtt tcttcaataa agctttgtcc gccgaaccca ggattgacag
    26281 tcatgactag tacttgatca gccaaaggca aaacatgttt gatcatggag actggtgttc
    26341 ctggattgat aactactcca gctttaactc ctaagctttt tgtcatttga agtgcccgat
    26401 gaatatgagg agttgcttcg acgtggacag taatgatatc tgctccagct tttgcaaatg
    26461 ctggaatgta gttttctgga tcaacgatca tcagatggac gtctaacggt aatttagtaa
    26521 caggacggat tgcctgaaca atatttggcc ctaatgtgat atttgggaca aattggccat
    26581 ccattacatc tacgtggatg taatctgcgc ctcctttttc cactaactca atgtctcttt
    26641 gtaaattggc aaaatctgca ctcaaaatgg atggtgctat cttcattctt gtttcctcct
    26701 attttcgttt accttctttt tttgcataca ttggttttct tttttcgatt tcttgtaaga
    26761 attgcaaata attgtcgtaa cgtgtctgtg cgataccacc tgtttctact ctatgtttta
    26821 cttcgcagcc aggttcttta gcatgcatgc actctctaaa tttgcaatgg acagaagctt
    26881 ctacaaattc aggaaattgt ctcggcagtt ctcctgcttc aattgtcaaa aagtcaatcg
    26941 agctaaatcc tggtgtatct gctactaacc cgccatagat aggaatcagt tccacatgac
    27001 gtgttgtatg ccgtcctctt ccaagtgcat cagaaatctc tcctgttgct aaattcagtt
    27061 ccggtgatat ttgattcagt agcgtcgatt ttcctgctcc agactgaccc ataaagaccg
    27121 atagccgttc tgggaagtaa cggatcagtt ctgtcgtatc acccgtatga ttgggtacaa
    27181 tcaccggata gcctatttta gtataggttt gcttgatctc ttcgattttt tctgggtcgt
    27241 ctgacaagtc ggtctttgtc aaaaaaataa tcggttcgat cgactcgtat tccaaggtaa
    27301 ctagaaagcg gtcaagtaga ttataagaaa atgcaggctc tacgatactt gtgacaatga
    27361 ttccaagatc aacatttgtg actggtgggc gaactaattg attttttctc ggcaagattt
    27421 ccagaagata gccgtctgtt tgattttcac tctcaaagat cacctgatct ccgacaagcg
    27481 gcgtgatttt ccgattccgg aaatttcctc ttcctcttgt ttgataggtc tctccgtttg
    27541 cgtatacata gtaaaaaccg ctgattgctt ttctgatttg tcctttgata agtgccacct
    27601 ccgtttttgt ttattttacc atacgaagag gaaagtaggt gttattccat gcaaacttct
    27661 caaagtatat ggcttgaaaa ttgatcaaaa gagatagaaa cacaaaaaag a
//
LOCUS       DACDCJ010000035        27651 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDCJ010000035 DACDCJ010000000
VERSION     DACDCJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.035"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IV038_001439"
     CDS             complement(287..649)
                     /locus_tag="IV038_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341667.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IV038_001440"
     CDS             complement(723..1103)
                     /locus_tag="IV038_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP8341668.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IV038_001441"
     CDS             1185..2360
                     /locus_tag="IV038_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8341669.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IV038_001442"
     CDS             complement(2437..3021)
                     /locus_tag="IV038_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8341670.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IV038_001443"
     CDS             3222..4220
                     /locus_tag="IV038_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8341671.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IV038_001444"
     CDS             4332..4841
                     /locus_tag="IV038_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP8341672.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IV038_001445"
     CDS             complement(4871..5530)
                     /locus_tag="IV038_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP8341673.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IV038_001446"
     CDS             complement(5532..6296)
                     /locus_tag="IV038_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP8341674.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IV038_001447"
     CDS             complement(6304..6981)
                     /locus_tag="IV038_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP8341675.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IV038_001448"
     CDS             complement(7018..8403)
                     /locus_tag="IV038_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP8341676.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IV038_001449"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IV038_001449"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IV038_001450"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IV038_001450"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP8341677.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IV038_001451"
     CDS             9342..10199
                     /locus_tag="IV038_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP8341678.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IV038_001452"
     CDS             complement(10280..10615)
                     /locus_tag="IV038_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341679.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IV038_001453"
     CDS             complement(10682..12052)
                     /locus_tag="IV038_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP8341680.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IV038_001454"
     CDS             complement(12130..12666)
                     /locus_tag="IV038_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8341681.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IV038_001455"
     CDS             12975..14456
                     /locus_tag="IV038_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8341682.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="IV038_001456"
     CDS             complement(14559..15266)
                     /locus_tag="IV038_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP8341683.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="IV038_001457"
     CDS             complement(15345..15683)
                     /locus_tag="IV038_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP8341684.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="IV038_001458"
     CDS             complement(15794..16756)
                     /locus_tag="IV038_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP8341685.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="IV038_001459"
     CDS             complement(16771..18102)
                     /locus_tag="IV038_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8341686.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="IV038_001460"
     CDS             complement(18110..18790)
                     /locus_tag="IV038_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP8341687.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="IV038_001461"
     CDS             complement(19009..19488)
                     /locus_tag="IV038_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP8341688.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19615..21294)
                     /locus_tag="IV038_001462"
     CDS             complement(19615..21294)
                     /locus_tag="IV038_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP8341689.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="IV038_001463"
     CDS             21842..23344
                     /locus_tag="IV038_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341690.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="IV038_001464"
     CDS             23357..24019
                     /locus_tag="IV038_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341691.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="IV038_001465"
     CDS             complement(24120..24323)
                     /locus_tag="IV038_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8341692.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IV038_001466"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IV038_001466"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP8341693.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IV038_001467"
     CDS             complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IV038_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP8341694.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8229 a         5770 c         4675 g         8977 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttgggaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt tatctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc
    27481 gtttcggttt tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag
    27541 taattttgat gaataaaaaa tggataatca aatgaattat acagtaaaaa tgacatttcc
    27601 acatgaataa tttattagaa aatcactttt ttctattttg tttttttctc c
//
LOCUS       DACDCJ010000036        27553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDCJ010000036 DACDCJ010000000
VERSION     DACDCJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.036"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(203..1006)
                     /locus_tag="IV038_001468"
     CDS             complement(203..1006)
                     /locus_tag="IV038_001468"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP8341695.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1026..1868)
                     /gene="kduI"
                     /locus_tag="IV038_001469"
     CDS             complement(1026..1868)
                     /gene="kduI"
                     /locus_tag="IV038_001469"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP8341696.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(1884..2891)
                     /locus_tag="IV038_001470"
     CDS             complement(1884..2891)
                     /locus_tag="IV038_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP8341697.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(2898..3542)
                     /locus_tag="IV038_001471"
     CDS             complement(2898..3542)
                     /locus_tag="IV038_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8341698.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(3642..4400)
                     /locus_tag="IV038_001472"
     CDS             complement(3642..4400)
                     /locus_tag="IV038_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP8341699.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(4527..5678)
                     /gene="fucO"
                     /locus_tag="IV038_001473"
     CDS             complement(4527..5678)
                     /gene="fucO"
                     /locus_tag="IV038_001473"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP8341700.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(5760..6071)
                     /gene="rhaM"
                     /locus_tag="IV038_001474"
     CDS             complement(5760..6071)
                     /gene="rhaM"
                     /locus_tag="IV038_001474"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP8341701.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(6073..6918)
                     /gene="rhaD"
                     /locus_tag="IV038_001475"
     CDS             complement(6073..6918)
                     /gene="rhaD"
                     /locus_tag="IV038_001475"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP8341702.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(6932..8188)
                     /gene="rhaA"
                     /locus_tag="IV038_001476"
     CDS             complement(6932..8188)
                     /gene="rhaA"
                     /locus_tag="IV038_001476"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP8341703.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(8185..9645)
                     /gene="rhaB"
                     /locus_tag="IV038_001477"
     CDS             complement(8185..9645)
                     /gene="rhaB"
                     /locus_tag="IV038_001477"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP8341704.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            9798..10667
                     /locus_tag="IV038_001478"
     CDS             9798..10667
                     /locus_tag="IV038_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8341705.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(10793..11692)
                     /locus_tag="IV038_001479"
     CDS             complement(10793..11692)
                     /locus_tag="IV038_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8341706.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(11857..12681)
                     /locus_tag="IV038_001480"
     CDS             complement(11857..12681)
                     /locus_tag="IV038_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8341707.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(12692..13987)
                     /locus_tag="IV038_001481"
     CDS             complement(12692..13987)
                     /locus_tag="IV038_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP8341708.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(14003..14482)
                     /locus_tag="IV038_001482"
     CDS             complement(14003..14482)
                     /locus_tag="IV038_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP8341709.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(14488..15516)
                     /locus_tag="IV038_001483"
     CDS             complement(14488..15516)
                     /locus_tag="IV038_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP8341710.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(15533..16639)
                     /locus_tag="IV038_001484"
     CDS             complement(15533..16639)
                     /locus_tag="IV038_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8341711.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(16868..19465)
                     /gene="adhE"
                     /locus_tag="IV038_001485"
                     /gene_synonym="adhC"
     CDS             complement(16868..19465)
                     /gene="adhE"
                     /locus_tag="IV038_001485"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP8341712.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(19770..20150)
                     /gene="yajC"
                     /locus_tag="IV038_001486"
     CDS             complement(19770..20150)
                     /gene="yajC"
                     /locus_tag="IV038_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP8341713.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(20247..21392)
                     /gene="tgt"
                     /locus_tag="IV038_001487"
     CDS             complement(20247..21392)
                     /gene="tgt"
                     /locus_tag="IV038_001487"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP8341714.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21610..22308
                     /locus_tag="IV038_001488"
     CDS             21610..22308
                     /locus_tag="IV038_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP8341715.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(22476..25115)
                     /locus_tag="IV038_001489"
     CDS             complement(22476..25115)
                     /locus_tag="IV038_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8341716.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(25734..26765)
                     /gene="queA"
                     /locus_tag="IV038_001490"
     CDS             complement(25734..26765)
                     /gene="queA"
                     /locus_tag="IV038_001490"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP8341717.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            26958..>27553
                     /locus_tag="IV038_001491"
     CDS             26958..>27553
                     /locus_tag="IV038_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8341718.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         7652 a         6131 c         4857 g         8913 t
ORIGIN      
        1 aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg
       61 aattccttct tatttcttgg agttaaatgc ttctgtccca gcctcttttt tcgaataaat
      121 gatgtttaat cagctgtcct tcggtattcg ttcaatccgc tgatttcaca acctctccac
      181 taaacagtgt actaaagctc ttttaaggtt gttttccgat ataggcaagt atgcctccgt
      241 ccacatacaa gacatggccg ttaacgaaat cagatgcgct agaagccaag aagacagatg
      301 gtccagccaa atcgattggc tcgccccagc gtgctgctgg cgtacgtcca acgataaatt
      361 gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg taatggagca gtttgcggtg
      421 tagcaatata acctggtcca attccattac attggatatt gtattgaccg tattcagaag
      481 cgatgttttt cgtcagcatt tttaatccgc cttttgccgc agcataagca ctgaccgttt
      541 cgcgtcctaa ttcactcatc atggagcaga tattgatgat tttaccgccg cctttttcga
      601 tcatatccgg aataactgct tttgaaacga tgaatggggc atttaaatcc acgtcgatta
      661 cttggcggaa gtctgccgca tccatttcga tcatcggtgt ccgtttgatg atccctgcat
      721 tgttgactag aatatcaact gaacctacat cttctttgat ctgttgaacc atcgcttgaa
      781 ccgcttcttc atcggtcaca tcgcagacat aaccttttgc atcgatgcct gcttctttat
      841 aatgcgctat accttcatca actgattctt gattcaagtt attaaatacg atttttgctc
      901 ctgctgtagc taaagagcgt gcaatctcaa atccaatacc atagacagca ccggtgacta
      961 atgctacttt tccatctaaa cgaaacatat ccatactaaa ttccatcatt ttctctccct
     1021 tatgcttatt ttaattgatc catttgcacc atatccatgt ctgtataggt gatattttct
     1081 ccgcacattg cccagatgaa tgagtaattg cttgtaccta cacctgaatg gatcgaccag
     1141 cttggcgaaa taatggcttg ttcattgtct acaactaaat gttttgtttc atctggtttg
     1201 cccatcatgt ggaacacacg tgtatcttca ttggcataat caaagtagac ataagcttcc
     1261 atacgacgtt catgtgtatg acaaggcatc gtattccaag cgcttcctgg ttctaaaatc
     1321 gtataaccca tttgtaattg acagctttcg catacatttg gatggatgta ttggtagatt
     1381 ttacgttcat tcagtgtcag gccttcacca gtctccattg gttttacttg gtcgatactg
     1441 atttttacat ttggatattt atgatgagct ggaacagaac taatatagaa tttagctggg
     1501 ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt cttttccgat atagtatccg
     1561 tcttgttttt tcatttcttc tttttgccca tcgatctcga tgaatcctgg tccaccgata
     1621 ttgatcacac ctagttcacg acgctctaag aagtaatcaa cacctagttc tttgtctaat
     1681 gtgatttcta attctttttc agtaggtgtc actccaccaa agatcattcg atcgttgtgc
     1741 gtataagtca aactgatttt ccctggttca aatacctttt ctactaaaaa ttcttttctc
     1801 aattgttccg ttgaataatg acggatatct tctggactgt gtgtataacg tgtttccatt
     1861 tcttgcattt tcttctactc cttctatcga atgatttttc cagagccgca tgctaaaaat
     1921 tgtgcgactt cttcttgact aaattggttg cagtccccat ggatcgtatg cttcaaggca
     1981 gaagctgccg tagcaaaatc aatgatttct tgcggagaca tattggataa aatcccatgg
     2041 agcacgccac ctgcaaaagc atctccgcca cctactcgat ccacgattgg ggtgattcta
     2101 tggacttggg attccacata ttcatggttc atccacaatg tccccttcaa ttcattttca
     2161 cttgctgaat gaacggtacg cttggtcgaa taaaacacct gaatattcgg gaacttttcc
     2221 tgcatttttt gataataata aattagaggt tgttcttctg cttctggcgc tttctcgata
     2281 cctaacaaat aaagagcatc catttttcca gctgagcaga tatctactaa aggtaaaagc
     2341 tgatggatta cttcgctggc ttcttgctgt gtccataatt ttccacgata gttgatatcg
     2401 aagctgatca ggcagccagc tttttttgca gcttctatca cctgtttcgt cattttttgc
     2461 cattttttag acaaagctgg ggtgattcct gaaacatgaa atatatctat tccttggaat
     2521 aactcctcta gcgaccattc caaatgatcc atttcagcaa aacttgatcc ggcacgatca
     2581 tagatcactg aggcggcgcg ttcgccacta ccagtttcca tataataagt acctagtctt
     2641 gatccaccag ataatacctg tttcgtatct actccataac attgcaagtg ctttttgact
     2701 gcttttccca atgcgttatc aggtactttt gtagcaaaaa tcgcttcatg tccgtaatta
     2761 gctaaagaaa ttgctacgtt tgcttcccct ccgccataat gggcagcgaa tcgttcactt
     2821 gaatcgatac gggttccctg ctctgtggac aatcgcaaca tgatttctcc gagtgtcact
     2881 acttttccca taagcagcta cctcttgatt tctttcattt ttgctgcata ctgttgcgcg
     2941 acttccgtta ctttgtcaaa atctcctgta gctgcgggtg ctaaaaggtt cccacctact
     3001 ccgactgtta cgacaccagc atcgaaccac tctttcatat tgtccagact cacaccgcca
     3061 gttggcatga tattgacttg aggcatcggc gccttgaagg cagaaatgat acttggtcca
     3121 taagcgcttc caggaaatag tttgacgata tctaccccgc ttttcagcgc tgttttgatc
     3181 tcggtaatag tcatacatcc tggtaagtaa ggaatttgat acagattgca catctctgcc
     3241 gtttcttgat caaaggaagg gctaacaata tactctgcgc cagctaagat agccaagcga
     3301 gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta tttctggttg gtctttgtag
     3361 aatgacaaaa gttctttgat tacttgatct gcttgaggga ctgtaaaagt caactcaata
     3421 ccagttagac cacctttgat gattgcgtgg caagcattca aagcttcttc tttgcttttt
     3481 ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag ataaaatatc aatttttttc
     3541 atcttctcca cctatttcct atttagaaat tttattttat aacttggaaa tctataccca
     3601 tattatacgg cagaaaagaa aaattgcaaa cgcttttttc attatttttg aattctttgc
     3661 tcgatttcgc tttttgtttg cttgagggca ctgattattt gttcttttgt ctcatctgtt
     3721 aaacgatatt tcggcatact tacactaaat gcaccaatga tttgttcgtt tttcacgatt
     3781 gaggctccta tacaaaaaat atcacgttcg atttcttcat cgtcaaatgc gatgtgggtt
     3841 tcttttaccg tttggatttc ctttttcaac cgtaatgcat tggtgatcgt atattctgtg
     3901 taagggatca acggatgctt ttcaagataa tcagtgtact gctcttcact gaattctgct
     3961 aatacggctt tgcccatcgc agaattgtat aatggtcggg tgattccgat ttttgaagac
     4021 atgcggatcg tttgatgttt tggatctagt ttgttcacgt acaagatctc attgttgtcc
     4081 aataccccta agtggatagt ctcatcgatc ttttgctgca gctgttctaa aaacggtaaa
     4141 gtcaactcta ccaaatcgat ttgttcaatg tttttattcg cgtagcgaat aagctttgca
     4201 cctaaacgat agtttttctc tgaattttta tttacgtagc cgatcatcaa caaagtatct
     4261 agtattttca agactgtaga agaggtcatt cccacaccct ttgcgatcgt ctgcaaagag
     4321 acatcttggt tttccgctaa atagtctagt attttagagg ctttgatcaa tacagttcca
     4381 taaggctttt tattttccat ataatattct cctatttctt tataagaaaa attatagcgg
     4441 aaataccatc aatttaaaag agatatccag atataaacta ccatttctct ttacaggcaa
     4501 tttaatgttg aagatccgct tcgtttttac atcaaacttt gatataaagc aatgatttct
     4561 tctactgtcg tttctcttgg atttcctggt gtgcatacat ctcgcaaagc atcttctgca
     4621 attgctggaa tatctgcttc ttttacacct aattcagaaa tggtttctgg gattccaacc
     4681 gctttgctca accgatcgat ctcaccacaa gccgcttctc ttacatcttc taaagacatt
     4741 tcagctgctc ctttaatccc tagaacaaga gcgatctcac ggtacttttc tccagtatat
     4801 tctttgttat atttcatgac agttggcaaa agtgctgcac acgctacgcc atgaggaatg
     4861 ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca gtcctaaacc gacatttgag
     4921 aagcccattc ccgcaatata ctgtcctaat gccatttttt ctcgcccatt ttgatcgcca
     4981 tctacagaaa cttccaaaga atggccgatg atttcgattg cttttaaatg cagcatatca
     5041 gtcatttccc aagcgccttt tgtgataaac ccttcaatag catgcgtcat agcatccata
     5101 cctgttgatg cagccaattt tttcggcatt cccatcatca tatcgctgtc gatgaacgcg
     5161 acgattggga tatcatgagg atcaacacaa acaaattttc ggtgatttac ttcatctgtg
     5221 atgacgtagt tgatcgttac ttctgctgct gttccagaag tcgttggtat agctaatata
     5281 ggtaaacatg gatttttcgt atcagcaaca ccttctaaac taatgacgtc tgaaaactct
     5341 ggattcgtca aaataatccc aatggctttt gccgtatcga ttggcgaacc tccaccgatt
     5401 gcgatcaaac agtctgcctc tgcctcttta acaaaagcca ctccatcttt tacattttgg
     5461 atcgttggat taggtacgat cccgtcgtat aaagcataag cgattgctgc tttatctaga
     5521 agttctgtta cttttgttgc tataccgtgc tcgatcaatc ctttgtcagt aatgacagct
     5581 gctttgccga atcctcgctg ctgaaattca gggacgatcg actcaatcgc tccttttcca
     5641 aaatacgatg tttcatttaa aatcattcga ttactcattt tctattcctc ctaatttgta
     5701 ttatttttca caataaaaac gaaagaaccc caagcaaccg gttgcccagt tgcttgttgc
     5761 tacagttcaa agactggcat caattcccaa ctgactggag aggcatctgg atttgtctcc
     5821 atgatatctt ccatgtactt ccaccatttt tgattgatag atgtatttgc cattttttcc
     5881 cataactttt catcttcgat cactaagtaa ccaaataaca aacttgtact cggatcaaga
     5941 tagatcgaat agtcgatgac cccgtgttca cgaagtgctg tacgcatttc tggccaaagt
     6001 tcatcatgtc tttttttgta ttcttcttgt ttttctgggt aaactttcat gcaaaaagct
     6061 tttttcacca tgtcagacac ctgctttcgt atgaacttcc tccaaataac ctgcttttgg
     6121 agtgacacca aacgcatctg ctaaatgcca caggttttcg tccgtgattg tttgcaagat
     6181 tggtccttgc gctttgacgt acgtatagac ttctgctgcc ttttctgctg tctcgatcaa
     6241 accaaagact tcatccatgt cttttcctga cccgtagatt ccatgttgtg gccataaaac
     6301 caatcgagtt tctttcattt tttcagctgt tgcttcccca atctcatttg ttcccggaac
     6361 catccacgga atgatggaaa ttccttctgg aaaaacaact aagcattccg tacacatttg
     6421 ccatagcgta cgactaaagg aacgttcgtc taattcatgc gtaaaactca tcgctaatag
     6481 atgactagca tggcaatgca taacgatccg attatctggg tctacttcta aacgggcgat
     6541 atgactcatg aaatgacttg gcaactcgct tgtaggaagg cattcgtttt ccagccccca
     6601 caaaagttcc agtgttttgc catcttcagc aactcgaacg attccaagat tctcggcagg
     6661 ttcgctcgct acatttttaa aatatcttcc tgaaccagtt acaataaaat agcatcctgc
     6721 taaacggctg gcatcaaaaa tcatcgagat gcggcggatc actttttcag gatccaagaa
     6781 cggtgtgatc tcttcttctt ttagtaaata agaaatattc ccgccatttc tttcatccca
     6841 tcccaagcga taaagattcg acgtcgtttc gatcatttct ttcacataag gcgcttgtaa
     6901 aatatctatt tttttcaata tagccacact cctattttct taattccaac acgtcttttt
     6961 gatattcctg gacttcttcc agccaatcca aaccaactgg aacttgattt ttgtaacaga
     7021 aataattcca tacatcggca tacgggaaat ctttcaattc ttccgtataa gccattcttg
     7081 aagtaaagtc aaaagccagc tccatttctt tcaatcgctc aactggttcc agcatagctt
     7141 tcagcagtgc tttttgtgta tttctcgtac cgatgaccca agcagcgata cggttgatcg
     7201 ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa caattcgtta cggactaatt
     7261 ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac atggtctgaa tcccaacgca
     7321 ctggacggga aacatgcaac atgattcctt tgctgaataa agctaatgaa gataatttat
     7381 tggaaatcac ttctgttgga tgaaaatgtc cagcatctag acaaacaagt ttgtcacgag
     7441 tcaacccgta acccatataa aattcatggg aaccgactgt ataagcttca gcgcctaaac
     7501 caaatagttt gctttctact gcatcttgta cataccgttc atctaactct tctgtaaaaa
     7561 tttcatcgag tgattccatt aaacgttccc gtggggtaaa acgatcaatc ggattatctt
     7621 tcatcccatc aggtacccaa aagttattga tacttgtttg cccagtttct ttcccaaagt
     7681 actccgcgat tttgcggctt cttttcccgt gttcgatcca gtattcacga acttctttat
     7741 ctggatgagc caatgtaaag ccgtctttca tcattggatg cgaaaagaaa gtcggattga
     7801 agtcaagacc gatttcttct ttttttgccc actctaccca tttttcaaaa tgtttcggct
     7861 caatttcatt tagatcgaca gattcttctg tatccaaata gatagcatga aggttcaatt
     7921 tgtgtttgcc cggtatcaac gaataagcct tctccagatc ttgtctaagc tcttcaggtg
     7981 ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc accggtcaat tcgccatccg
     8041 gtgtcaagaa acctttgaca tcatctcctt gccagcaatg catagaaatt ttgatgtcct
     8101 gtatcttttc tagtacagca tctgtatcta caccgatagt tgcatatttt tcttttgctt
     8161 gttcgtaatt tctttcaatc gtgttcatac gattactcct ttgattttat tcacgtacca
     8221 ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt tcacatggaa acgactgttt
     8281 gattacattt cttgcttgtt ctatattcga aaactgacct gtactgagca tttgcatggc
     8341 aacattcccg attgctgtgg cttcactagg tcctgcttct acaatgattc cggcataatc
     8401 tgctgtcaat tgattcagta gacgattatt cgatccgccc cctacgatgt gcagcttttc
     8461 caatttcgtt ccagttaagt gttctacttt gatcagctct tgtgcgtaac aaagagcaag
     8521 attatcatac acacaacgag cgatagctcc cgatgagaca gggactggct gctctgtttc
     8581 aaaacagtat tgctgaatct cttctatcat atttggggga ttcaaaaatc gagggtcatt
     8641 gatttccatc acttgctgca gcggtttttc ttttgcagca agctctgcca attccgcata
     8701 actgtactcg tactcttgca ttcgggcaac ttcctggatc aaccacatcc ccatgatgtt
     8761 tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc gtataatttt cagaaaaagc
     8821 attgcgagaa acattggcga tctttgtttc aatccctaaa agcgaccatg tcccactgct
     8881 gatataaccc caacgttctc cttgacctgg cgttcctaat acagctgacg ccgtatcatg
     8941 acttgctact gtgataaagg tcacatctgg caaatcatat tttgaaaatt gttctttttg
     9001 cagatctcct agtatcgttc ccggctctgt caatggtgga aataattcct tcgagacacc
     9061 gattttttca agaagttcta tttcccattc ttttgtccct gcattcagca gctgcatagt
     9121 agatgcatta gttttctccg tcaccatgtt accagtaaag acatacccta aataatcagg
     9181 tatcagtaac aatttatgtg cttttgccaa attttctttt tcttcagcaa acaattggaa
     9241 caaggtattg aaaggctgga tctgaatccc tgtctgctca tacaacttat ctagagaata
     9301 gatttcagaa aactgttcta ctgctccatt ggtacgcttg tcccgataag caatcggatc
     9361 atttatccga tccccttgtt catcaatcag acagtaatcg actgcccatg tatcgatccc
     9421 cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca aatccaatca aaatctcttt
     9481 gataagataa tcgatcttcc agcgatcgtg accgtctttg ttccgaaagc cgtttttgaa
     9541 ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc ctcgtacttt tcattagtcg
     9601 tccgcttgat gcaccgatat cgatggcaac aaatgttgga ttcatttctc cacctctttt
     9661 actttattgt tgattagcac tttattaata aagcgttatc atttattaac aatgctatca
     9721 taatacagaa tcttatattt tgcttcacac tattttggta tataattgca ttatcttgac
     9781 aagttgggag tgtttacatg gattactggg aaaaagccaa ccactcatgg tcggaagact
     9841 caaaaagagt gatcttgacg cctacacaaa gaagcaggaa tttatttttt tatatccaag
     9901 aaatcggtta cttcaaagca ccaatccctt actatactga acgagcacat ttaccttctt
     9961 acttgataaa gtttactttg tctggcaaag gttcgcttac atataatgat aatcagcaac
    10021 agctaaaagc tggagactta ttttttattg actgccaaga atatcagcgt tatcaaacga
    10081 tcagcgaaga accatgggaa atggattgga tccatttgaa tggtcagaac gtccaatcat
    10141 tttatcagga gttcacaaaa gacggccata acaccttcca ttcaacaggg gatccatcag
    10201 aaaacaagat ccaccatttg atcacagaat tgatccacat gcaacaaggc acgaatgccc
    10261 gtaccgattt ccagtcctct gtagctatcc acgagttgct gaatgaactg attttgcaaa
    10321 agtatcagct agatttcgaa gaagaggata ttcccgccta cgtttacaaa ctgaagcaat
    10381 acttagacga acatttcaaa gaaacgatta cacttgatca attagaacaa caattcttat
    10441 tgaataaata tcagctgagc aaagattttt ccaaatttat cggttctcca ccaatcgatt
    10501 atttgatcag caaaaaactt tcttacgcta aagaccttct tcggtataca gaatttacgg
    10561 ttcgaatgat ttccgaagaa atagggattg agaattttgc ttactttagc cgcctgttca
    10621 aagcaaaaac aggactatcg ccgagagact ttcgtaaatt cagctgattc atataatatg
    10681 tacgtcattt tttcaaaata ttttgaaata tatagataaa aataaaataa ccgcagtttc
    10741 tgcttctatg taaacaacag cagctactgc ggttattttt tagtgatcat tctcattgtt
    10801 catcttcaaa atgttcataa tgaaccaact gtttctgtgt atcacgtttc aaaaattctt
    10861 taggcgttat gccgaactct ctcttgaatg ctttcagaaa atgcggataa gaagaaaaac
    10921 cacttttact atatacagca ctagagctgc ggtattctcg caacaagtgc ttggcataga
    10981 tcagcttttt cttcattaca tactgatgaa acgtaaatcc agtgtacttt ttaaattctc
    11041 gtgtgatatg gtatttgctg acaaaaaact gcttttccat ttcctctaaa gaaagcggat
    11101 gagataaatt ttttgagata tattgtaaca tttgttccac acgctcatta ggaactacag
    11161 cattctgttc ttgcgcactc tcttcttttt gtacaacttg attgagaaaa atcaagtatt
    11221 ctaccagagc ttgttcatat tttatgtctt caccgtaaat ttttttatca ggaggatggt
    11281 cgataaatga taatttttgc ttcaatactt cctgttctat ttgcaataca cgactcctct
    11341 tctctcccat tggttgaaaa caagccgata gattagacca tggcgtggaa cgactttcta
    11401 gaaaagaagg ggtgacaaag atatagacac gctcgaatac atctgaagat tggcggacga
    11461 tacgatgcaa atcattggaa tggatgagca gcacacttcc tgctttgatc gtaaattgtc
    11521 gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc atggatctca tagaaatcat
    11581 gatgatgata gatcgtttta ttttggctga cgacatcttt gacatgatag atttcatact
    11641 gatcagaaac catcgtattg acttgttcac cgctgatttt cttttggagc atgtgccgca
    11701 cctccttttg ttcacttatg cacaatttta ttataccacg ttttttgtaa tcaaaaagag
    11761 ctgcttttat atactcttat aaaaaagaag agacgtggca tcttctagcc ccgccccctc
    11821 cattctaaat attagttaac caacgtcttc tttacattat tcaagaagac ttcttccata
    11881 acgaatccca tcatcttttg tttcttcaag aaccacatac aggtacggac gattcttcat
    11941 gatgatttct ttgatttttg tgtactcgat cacaccgtct cccaaattta ccggcacgat
    12001 tttttcctgt tcgacacgaa aatctttcaa atgaaccgca catatctttt caccaaaacg
    12061 ttcaaaagct tcttctacca attggacttg ttcttgataa tcactacttg tgatcaaatt
    12121 ggtcggatct aaaatgattc ctagatagtc cgaatctact gcttggatca gctgctctac
    12181 ccttgctaaa gaatacaccg gatgattcaa acctggttct atccccacca tcatttggta
    12241 tttttctcca gcttttacca agcgacggat aacggacaca gcttcagcaa atgcttcatc
    12301 tgtgaaattt tcttccgtat attggatttc cggcaaaaca catccagttt ccgaagcaac
    12361 catcgacgca ccaaaggaag aagcatagcg aagatactgc tcgaactttg tcagcaactc
    12421 ttgtcgtatc gttaaatctg gatgaatcat attgatatag caacttaaaa ttcctacggt
    12481 cacttcttgc ttttctaaaa ctcgtttgac ataatttccc atcccagaat tgatctcatt
    12541 tgcgccagaa ggtgtttcag gaaacgaaag acctaaggcg agctgcagtg tatgaatccc
    12601 ttgttctttt gttttttctg caagatcttc tatcgtttgg acttcggaaa gatcatgtcc
    12661 tctaattgta atgttcaaca taactattct cctaactcag catattgggt agccacatga
    12721 cgatttgcgg gaagtaagta atcaaaaaca agacgactaa gataacccca taaaaaggaa
    12781 gaagcggttt cagcattgcc tctgctttta cgcctcctac acttgcaccg acatatagtc
    12841 ctgtacctac tggcggcgtg atggagccta cacacagatt catgattgaa aacaacccgt
    12901 aatgtacagg atcaacccct acacttgtga cgattggtaa aaatattggt gtaaaaatca
    12961 agatagcagg cgccacatcc ataaacatac ccaccagtaa taacacaaga ttgactaata
    13021 acagaataat aaatttatta tccgttacgc ccataatcaa cgaactaatt gcctgaggaa
    13081 taccggtaaa cgccatggcg aaagacatca tggaagaagt agccaataag aacatgatcg
    13141 tccctgacat tgctactgat tgaaacaaca tattaggtat atcttttagt tttaccgttt
    13201 tatagtaaaa catagcaatc aacagggagt aaatgacagc tatcgctgaa gcttctactg
    13261 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    13321 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    13381 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac aaagcgccta
    13441 cgagataacc tcccataaac aaagcagaga tcgaagcacc accagcaaca agtgaataca
    13501 tgataaaagc agtacttgga ggaatgacca tacctgtcgg agctgacgcg atattgaccg
    13561 ctgtcgcaaa cttccggtca tatccttctt ccacttgctg agggatcaac actccgccaa
    13621 tcgcggtaga agcggcaatg gccgagctag agatggaacc aaataaagca ttccctaaca
    13681 cattcgtatg agccaaagcc cccggcacgc gcccgacgaa caacatcgca aaatccacta
    13741 gtttcttagc tatccctcca ttattcatga taatgcccga gagaacgaaa aacggaatag
    13801 cgatcagcga aaaactattg atacttccca ccattttttg agctgaacta aaagcagaaa
    13861 catcaaacgg tataactaac aataaagtca tcatcgatga gaatgcaata cttatagcaa
    13921 tcggcatccc taatacaagc aacacaacaa atacaaaaaa taacactaat ccggcttcta
    13981 ttgccatttt attcctccat ttttatattt tttcttgaat tgctggcttg ttgcttaatg
    14041 tatcgattag attgatcagg ctatagataa tgatgagtac ccccgataca ggtactgaca
    14101 gataaacata tcccatagga atacttaaag aaggcgagat ctgccccatt gtcaatgtga
    14161 cagcattcca tccgccatac accatgatca tcgcagcaaa taccagaaat aaactttgaa
    14221 caacaacttc taaaatttta ctttttttgc cagttaattt ttcacttaaa accgttacgg
    14281 ctaaatgtgc tttttgtccc acaacatacg cggaagacaa catggcaaac cagacaagac
    14341 cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc taaaaccgtt ctggaaaata
    14401 cttgccataa aacaacagcg accatcccaa caagcaaaac agctcccagg atctctaata
    14461 ccttgtttaa aatcattttc atcacagcta cgctccttgc tcttggatct tttgataaag
    14521 gtctttgtag gtaggatcgt ttttgaatat atcatgaagt ggggcaactg cctctcgaaa
    14581 cggtgtatta tctacatcat tgaattggac tccatgttct tcttgtgcta aagctttttc
    14641 ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc gttgattctt tcgctgcttc
    14701 ttcaatggct ttttcctgct catcagttag ctgttctttg atggcatcat tcatgatgat
    14761 gatatctggc acacgtgtat gctcatcata ggaatagtat ttagccacac caccatgtcc
    14821 agctgtatac aagacaaatt cattgttttc tgatccgttg atcaaattgg attgaagaga
    14881 cgtgtacact tcatcacttc ccataggtac gggcgaaccg cctaaaagct ctaccatttt
    14941 gatagcggtt tcgctttgca tcacacggat cttctttccc tccaaatctt ccggcgtatg
    15001 gatcggaccg tctaccatat aaaagctgcg ctgaccagaa tcataatagg tcaatccggt
    15061 aaatcctaaa tttttggtcg attggtagat tggctccatt atttcttcat tatccatcac
    15121 cttaaagaaa tgttcttcat cattgaatag ataaggaact gaaaaaatcg aataatcttt
    15181 agagaaactt tccaacgctg aggcacttac ttttgtgaaa tctactgctc ctgactgtgt
    15241 caattcgatc agttcacgtt ctccacccag ttgtccatct gggaaatact ggatctgtac
    15301 ggaaccatct gttttttctt ttactaaacg cccaaattca ttcattgcat caattactgg
    15361 ctggctgttg cttgcataag caaagcgcaa ggtaactatc tcatttacat ctgcatctgc
    15421 gacagttgcc acactagaat tatttccgca gccgcttaga cctagcagac ctaggctaag
    15481 cgaacttgct gcaaacagca gtgatgtttt tttcatatgg gtttcctttc ttttaacaat
    15541 aagagacgag caattctgtc aaacaaagca ccgttaacga ctgtccataa ggcattgctg
    15601 tgattccgat attccgataa aaatctaaac tgtcccccat tcctgtcccg attgaaacgt
    15661 tttgaacttc tcctttttca tcgatttgtt caagcaatcc ttggatagca cgataagcga
    15721 cttctttata ttcttgtggt aaataacgtt tatggaccgc tttcaaaata ccgtaagcaa
    15781 atcctgcagt tgcagaagat tctacgtaag aagaaggatc atccaacaac gtatgccata
    15841 aaccgctttc agactgataa gtttttaatg cacgcacttg tgcttcaagt gtttcgatca
    15901 ataatttgcg caatgcatct tctttcgata attctaagat ttcaatgatc tctggaatag
    15961 caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc ggcatagtta tgatttcctt
    16021 caaacgtcca gccgtgatac cacaatcctg ttcgtttatc catcaagtat ttgatatgga
    16081 tcataaactg gtaacgagct tcttctaaat attctgggcg attcaacagt ttcccgattt
    16141 ttgccaaagg aagcactgtc atcatcaatg tatcgtccca caactgattc ttattttccg
    16201 gtccatatgt ggcatgctgt aacccgtctt catttgtccg aggcatgtca ttcatgaccc
    16261 attctgccca ttgttctaaa taaggcaaat agcgtgaatc tttcgtgtct tcatacaagt
    16321 aagccatagt caatagcggt gccattgtat tgacgttttt gggcggagcg ccttcttcca
    16381 tacgatcctc aaaccattca ttgaccactt gataagcgcg aggatttttt gtcatctggt
    16441 aatttttata aataccatat agaccgacac cttgtggcca attccacact gtccagcttt
    16501 tgtcatctac tttcagtcca tcaaagtcca acaaaaattc accattatcg tctttgatcg
    16561 ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac taattcttta ggtacaaaaa
    16621 tctcttcttt aataatcatc tttacgccac ctttttaaac gctttcattt tatatcctta
    16681 atatagcagt aataccgtcc actataaata tcgctttttg aacaaaaact gtctaaaatt
    16741 gacctaaatg ttaaataaca aaagtaactt tcagttactt ggaaattaaa aaaagaactt
    16801 ggagaaacaa cgttttccaa gttctttttc atctattttc ttatcttttt tctacgcttt
    16861 cttttgctta tccgttgtaa gcatcaatga tgatctgttt caattcagag atcaatggtt
    16921 ctttcggatt agctgtcgta cattggtctt cgtaagctaa ttcagccata cggtctacgg
    16981 ttgtatctag tgtttcttgt gtgactcctt gagcttttag actcatgttg atgccgacag
    17041 attttcctaa ttcataaaca gccgttgcta aagcttcgac taattcctct gttgtttcgc
    17101 ctttcagacc taagaatttt gcgatatctg cgtaatctgt atctgcacgg aaatagtcat
    17161 atttagggaa catcgcatgt ttttgtggat cttttgcatt atagcggata atatgcggta
    17221 aaagaatcgc atttgtccgt ccatgaggga tcccgtattc cccgccgatt ttatgtgcga
    17281 ctgagtgaca aatacctagg aatgcgttag caaacgccat accagccatt gtagaagcgt
    17341 tgtgcatttt ttcacgtgat tccatatcag gtgtttttac tgatttttct aaatagtcaa
    17401 agactaactt gattgcttgt aggctcaatc cacgtgtata atctgaagcc atgactgaaa
    17461 cataggattc gattgcatgt gttaacacat ccatcccggt atctgctgtg acagatgctg
    17521 gaactgacat gacgaattga ggatcgacga ttgcgacatc tggcgtcaat gcataatctg
    17581 ctaacggata tttcacatgt gtttcactgt cagtgatgac cgcaaatggc gttacttcag
    17641 aaccagtccc tgatgttgtc gggatacata cgaattgtgt tttttctggt ttgtcgatct
    17701 tatatgtccg tttacggata tccaagaatt tttgttttgc accaaagaaa gatgtgtctg
    17761 ggtgttcata gaacatccac attcccttag ctgcgtccat tgcagaaccg cctccaagag
    17821 cgattactgt atctggcttg aagtctgcca tcagtttcgt tcctgcgtat acggtatttg
    17881 tcgatgggtt cggttcaaca tctgagaaga cttcgatctt cacatcattt ttacgtcgtg
    17941 ccaatacttc acgaactgta tcgcagtaac cgaattggac cataccaggg tcacaaacga
    18001 tcatgacacg ttcaacgttt tccattttct ctaagtacaa tagagagttc ttttcaaaga
    18061 agatttttgg cggtaatttg aaccattgca tgttatttct ccgtttcgct acagttttga
    18121 cattgattaa gtttacagca gagacgttac gagaaacaga gttcttacca taagaaccgc
    18181 atcccaaagt caatgacgga atcatttcgt tatagatatc accgatgccg ccttctgcag
    18241 atggtgtatt cactaaaata cgacaagctt tcattcgaag accgaatttg acatgcaact
    18301 cttcgttttc tgaatggata actgctgtat gtcctaatcc tccaagattc aacatacctt
    18361 cacacaaatc aaacgcatgg tccgtgttgt tagctttgat catggcaaga actggagaaa
    18421 gtttttctct tgatagcgga taatctgggc ctacgccttc caattcagca atcagcattt
    18481 tcgttccttt tggtacactg attcctgcca attttgcgat ttccatcgca gaatgtccaa
    18541 cgattgatgg attgaccgca tattttcctt cgttcatcac tgcatcttct aatttttgca
    18601 attcatctgg ttttacgata taaacttggt gtgcttggaa ttctgctttt actgctgcat
    18661 agatttcttt atccacgatc acagcttgtt cagaagcaca aatcattcca ttatcaaatg
    18721 ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt tgctgttttt tcaatataag
    18781 ctggtacgtt acctggacct acacctaaag ctggttttcc tgtagagtaa gcagacttga
    18841 ccattccagc tccacctgtc gctaagacga tcgcgatacc tggatggttc attagcatag
    18901 aagtcgcttc gatcgaagga tgttcgatcc attggataca attttcagga gctccggcag
    18961 cgatagcagc atcacgtacc actcgtgctg cttctgcaga agatttttgg gcacttggat
    19021 ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat cattgatttg aaaatcgttg
    19081 tggaagtcgg attcgttgtt ggagttactc cgcaaaccac accgactggt tcagcaattt
    19141 caatcaaccc tttttggaca tcttcattga tcacaccaac tgttttatca tgtttgatat
    19201 tatgccaaat atattcggac gcatacatat ttttgattgc tttatcttcg taaacaccgc
    19261 ggcctgtttc ctctacagcc atcttcgcta atggcatatg ttgatccaaa gctgccattg
    19321 ccatttcatg cacgatatgg tctacttttt cttgatcaaa gctttccatt tctttcaatg
    19381 ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg tacgtcaatt gtcttcgttt
    19441 tttctttttc tattacttta gccatcatta tcctcctaat gggtcatggt tatttgttaa
    19501 ataattcaca tgtatatcat acaatattta tttttctatg taaacacttt tttgtgattt
    19561 ttttcacagt tatttttaaa tataaaattg ctttatttac aggtgtttgt atagaaaaat
    19621 atataaagtt atttattatt atttatatga cacacccgat tattcaatat tgagcaatat
    19681 atcccatgct cttatctctt tcttttttga ataaaaaatg aggtaggaca tctttctgcc
    19741 ctacctcgtc tttaaataag ctttgatact tatttttctt ccttctcttc tgtctcttcc
    19801 agcgtttctg ttgtttcagt ttttacttca actgtctcag ctggttcttc tgttgtttca
    19861 acagtagtcg ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt gattgctgca
    19921 cgatcgtact caaggaagat cccttcgcag tctaaaacaa ctgtccgttt gtcattgtcg
    19981 atttctgaaa caactccatg taatccgccg atcgttacga ctgaatcacc tggtttcatg
    20041 ctatctaaca agttttgacg ttcttgctgt tgtttctttt gcgaacgtga cataaaaaac
    20101 cacattgcta atagcgcgat aaacatcaca agcattggta atgttcccat ttttttcacc
    20161 tcactaattc tgtatctaca aaaaacactt tagcagaaaa atgctgataa cactagtttt
    20221 ttacttaatt tttaaaagat tttcctttag aagctttttg cgttttcttt attgaacccg
    20281 tattcttcaa aaaaggcttc tctaaattca agcaaattat catccatgat tgcttgacgt
    20341 acttgtttca ttagattcaa caagaagtat aaattgtggt aagaagtcaa acgaatccca
    20401 aatgtttcat cacatttgat caaatggcgg atataggcac gtgtatagtt tttacatgta
    20461 taacaatcac atttctcatc aattggacgg aaatcatggg catatttcgc atttttcact
    20521 actagacggc cttgagaagt catacatgtg ccatttcgag cgattcgagt aggcaagaca
    20581 cagtcaaaca tgtctactcc acggataaca ccatcgatca atgaatcagc agcaccgaca
    20641 cccatcaagt aacgtggttt gttttcgggg atcaacggtg tagtgaattc taatacacga
    20701 ttcatttctg atttaggttc cccgacagat aacccgccga ttgagtaacc agggaaatcc
    20761 atcccgacta gatctttcgc gctttgacga cgaagatctt caaaccctgc tccttggatg
    20821 attccaaata atccttgacg gtctggatta gcatgagctt tcaatcctcg ttctgcccat
    20881 cgagacgtac gttctactga ttttttcacg tagtcgtaac tttcatcgaa tggtggacat
    20941 tcgtcaaaac tcatcatgat atcagagccc aatttatttt ggatgttgat tgctttttct
    21001 ggtgataaaa acatacgcga accgttcaag tgatttttga aatgcacgcc ttcttcttcg
    21061 atattccgca tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt taaaatcggt
    21121 tgatcccagt tcataaattt atgcagtccg ccagcttctt ctactaagtc ttcaccaggg
    21181 cgtagccata aatgatatgt attacttaaa ataacgccag cacccatttc tttcaactct
    21241 tctggggaca ttgtttttac tgtagctaat gttcctactg gcatgaacat tggtgtgggg
    21301 aaagttccgt gtggcgtgat caattcccct aaacgcgcgc cagtatgttt ttctttcttg
    21361 attaaacgat agcgaatggc aggttgcgtc attcatttcc ctactttcta tataaaatat
    21421 cttgacaact cccaccatca taatcgtttc aagtgctttt tgcaagttcc ctcggcttcc
    21481 ttctacttta tttaatattc ttttttttat ccttttttta tgaataacaa aaaaaagaaa
    21541 aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa agaaaaggag
    21601 ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag tctaaaagga
    21661 cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat tgctactatt
    21721 cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg atcagcgagc
    21781 gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg aattatttcc
    21841 agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga tattttacgt
    21901 ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa aggtttgttt
    21961 atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt atggggtttg
    22021 ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta ttttatttac
    22081 tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat cactgcaagt
    22141 cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc atttatcggc
    22201 tggcatatct tagcattagc aactgctggt attggttatc tatggctcag cccatacatt
    22261 tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa gagagtgtga
    22321 cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa aagaaacatt
    22381 cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt tttgtatagt
    22441 tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc tgcttccggt
    22501 aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa aatccctacc
    22561 aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt aaatggcagc
    22621 aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa agctttattg
    22681 gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac atgcagaatc
    22741 gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact tgcgtctacc
    22801 catgggctga tagtatcgac ataactacct aaataataac caaacaaggt tacgatggtg
    22861 aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat cccttcattt
    22921 ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc taaagcaaat
    22981 cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt aaatggtgct
    23041 ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt acagcttagc
    23101 aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact gacagcgtct
    23161 acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg cgccacatct
    23221 gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg tgcatcattc
    23281 acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt gacgatccgg
    23341 atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg cgcatctaat
    23401 tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg atcattcaag
    23461 atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt gatcatgact
    23521 gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg acgtggtgga
    23581 tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc agaagaaata
    23641 tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag tgcccgttta
    23701 ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa gcgcggagca
    23761 agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg gtgaactgtt
    23821 gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata tttttcttct
    23881 aattctgcct ttgtatgata attctcttca acagccctta cgatggctac ttctgttgga
    23941 ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt acatagtgca
    24001 gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt cgttacttca
    24061 tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt tcctgttttg
    24121 tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg tcggatgatc
    24181 gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac aataacagtc
    24241 aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat ctcaaggagt
    24301 ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa agcgatcata
    24361 ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg catagtattt
    24421 tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc agtgacgatc
    24481 gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag ttggtcacct
    24541 aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc actttctcca
    24601 gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc atcggcagaa
    24661 atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc tgactcaaca
    24721 gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc ttgcaaggat
    24781 tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt gatcacaacg
    24841 atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa tgcggtataa
    24901 aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa gcttcgaaag
    24961 aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc ctgctttttt
    25021 cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc tgttaccttc
    25081 tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta aaatcttcgt
    25141 acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca aataaagggc
    25201 ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc aaacgctacc
    25261 aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac agaagaattt
    25321 ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac ccagacatgc
    25381 gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca cgaagtatcc
    25441 gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga aaattgaata
    25501 ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt gtttaacatg
    25561 aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt tctgtttatt
    25621 tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt ttctttgtca
    25681 taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata accttatttc
    25741 acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc atgctggtaa
    25801 gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa agtcgatttt
    25861 ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc aggcgtaatg
    25921 aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt tccaatcgtt
    25981 tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt tttacgtact
    26041 tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg catatggtgt
    26101 tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg tagtgtcaga
    26161 tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt aaaatgcaat
    26221 ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg atagcgatct
    26281 gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa tgattcaaga
    26341 atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg ttccaattct
    26401 tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc aactttggca
    26461 cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt tagaagaagg
    26521 acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg cagaactcgt
    26581 gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc atggaaatgt
    26641 ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg atcgcggtct
    26701 tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc ttctgttgtt
    26761 aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt tttcgttctg
    26821 ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa aaaactaccc
    26881 tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc attgaaaata
    26941 gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat aaaggtggaa
    27001 aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt tgttttatcg
    27061 gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg actgatccaa
    27121 cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca gtggaattgc
    27181 gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg acgatccgtg
    27241 aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg aataaaacag
    27301 ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc tacccacatg
    27361 aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca gcgaatcaag
    27421 atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt gattccttgc
    27481 aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt gtaacccatg
    27541 acatggatga agc
//
LOCUS       DACDCJ010000037        26150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDCJ010000037 DACDCJ010000000
VERSION     DACDCJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.037"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            227..1231
                     /locus_tag="IV038_001492"
     CDS             227..1231
                     /locus_tag="IV038_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341719.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1232..2176
                     /locus_tag="IV038_001493"
     CDS             1232..2176
                     /locus_tag="IV038_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341720.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2176..3138
                     /locus_tag="IV038_001494"
     CDS             2176..3138
                     /locus_tag="IV038_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341721.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3165..4079
                     /locus_tag="IV038_001495"
     CDS             3165..4079
                     /locus_tag="IV038_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341722.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4105..5886
                     /locus_tag="IV038_001496"
     CDS             4105..5886
                     /locus_tag="IV038_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8341723.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNDAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6234..6920
                     /locus_tag="IV038_001497"
     CDS             6234..6920
                     /locus_tag="IV038_001497"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP8341724.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6940..10521
                     /gene="smc"
                     /locus_tag="IV038_001498"
     CDS             6940..10521
                     /gene="smc"
                     /locus_tag="IV038_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP8341725.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEVLAKEQPKMYQLMNQVQQLRARQKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIHYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCSVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10530..11339
                     /locus_tag="IV038_001499"
     CDS             10530..11339
                     /locus_tag="IV038_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8341726.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11351..12349
                     /gene="ftsY"
                     /locus_tag="IV038_001500"
     CDS             11351..12349
                     /gene="ftsY"
                     /locus_tag="IV038_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP8341727.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12536..14803
                     /gene="gshAB"
                     /locus_tag="IV038_001501"
     CDS             12536..14803
                     /gene="gshAB"
                     /locus_tag="IV038_001501"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP8341728.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTGRNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFADQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14892..15344)
                     /locus_tag="IV038_001502"
     CDS             complement(14892..15344)
                     /locus_tag="IV038_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP8341729.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15494..16444
                     /locus_tag="IV038_001503"
     CDS             15494..16444
                     /locus_tag="IV038_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8341730.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLAT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16437..17396
                     /locus_tag="IV038_001504"
     CDS             16437..17396
                     /locus_tag="IV038_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP8341731.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     GFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17393..18148
                     /locus_tag="IV038_001505"
     CDS             17393..18148
                     /locus_tag="IV038_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8341732.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18171..19124
                     /locus_tag="IV038_001506"
     CDS             18171..19124
                     /locus_tag="IV038_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8341733.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPNLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19647..19943)
                     /locus_tag="IV038_001507"
     CDS             complement(19647..19943)
                     /locus_tag="IV038_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP8341734.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20298..20654
                     /locus_tag="IV038_001508"
     CDS             20298..20654
                     /locus_tag="IV038_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8341735.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20664..21563
                     /locus_tag="IV038_001509"
     CDS             20664..21563
                     /locus_tag="IV038_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8341736.1"
                     /translation="MGNLAIISDLHVDINKFGEPELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFANKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22080..22811
                     /locus_tag="IV038_001510"
     CDS             22080..22811
                     /locus_tag="IV038_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8341737.1"
                     /translation="MMKKEIVLTAATMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGPGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRGTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYMPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22956..24983
                     /locus_tag="IV038_001511"
     CDS             22956..24983
                     /locus_tag="IV038_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP8341738.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISVVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENENLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDVEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25091..26150
                     /locus_tag="IV038_001512"
                     /pseudo
     CDS             25091..26150
                     /locus_tag="IV038_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
BASE COUNT         8708 a         4560 c         5557 g         7325 t
ORIGIN      
        1 tttttatcct tcttttttta atctttttga cacgtaaaag tcatttttct gataattctg
       61 gcgataaaca gaaattatgt tgcaaagtaa agcggttttc tatataatta ttaataattc
      121 attacacaaa gataagaata acctttgtta taaaacgatt tattctgtgt aatgtccttt
      181 tttattaaga aaaacttcca tttaggaagc agagaggggt attcgattgt ccgataacca
      241 attattagaa gttcagaacc tacatacagg attccgactc aaagatgact attacgatgc
      301 agtagatgac gtttcattta ctttagacaa aaacgagatt ttagcaatcg ttggggaatc
      361 tgggtgcggg aaaagtacct tggctactac gattatggga ttacttgatc ctaataacac
      421 aaaaattaca ggtgagattt tatataacga tctgaattta atcgacctga acgaaacgct
      481 ctataataaa atccgcggaa atgatatcgg aatgattttc caagatccgt tgtctgcatt
      541 aaatcctctc atgagaatcg aagaccagat caaagaagga ctttcctatc acacaaagat
      601 gaatgcagaa cagcgtcaag ctcgtgcact agaattgcta gaacaagtag ggattcctaa
      661 tcctgcgaga gttggacgcc aatatcccca tgaactatca ggcggtatga gacaacgtgt
      721 catcatcgca atcgcaatcg cctgcaaacc tccaatcgtc attgcagatg aaccgacgac
      781 agccctagat gtaaccatcc aagcacagat tcttgattta ctgaaagacc ttcaggaaga
      841 aacaaaaaca gggatcatct tgatcactca tgacttagga gtcgtagcag aaatggctga
      901 tcgagtggca gtgatgtatg gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt
      961 tgatcatcct aagcatcctt atacacgctc tttactgaac tctataccac aagaaggaaa
     1021 tcatgatgca gaactccatg tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg
     1081 taaaggttgt cggtttgcac cacgtattcc atggatacca gcaagcgatc atgaagaaga
     1141 gcctatcctt catgaagtcg caccgaacca cttggtacgt tgctcctgct ataagcattt
     1201 ccactttaga gacgaaaaag gagaggtgta gatgacagaa ttaatcacaa tcaaagacct
     1261 gaaagttcat taccctattc gcagtggatt cttcaatcga gtaacggatc atgtatatgc
     1321 tgtagatggt gtggatttta ttatcgaaaa aggaaagaca tatggtttag tgggagagtc
     1381 tggctctgga aaatctacta cgggaaaagc cgttgtggga ttagaaaagg taacagccgg
     1441 acaaatcatg tatgaaggaa aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta
     1501 taacaaggat gtgcagatga tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg
     1561 agtgttggat atcattgctg aacccatccg aaattttgaa cggttaagcg atcaagaaga
     1621 aaagaaaaag gtaaaaggac tgctagatat cgttgggatg ccggaagatg cgctatataa
     1681 atatcctcat gaattttcag gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc
     1741 taccaatcca aaactgatcg tagccgatga acctgtatct gctctggatc tatctgttca
     1801 agcgcaagta ttgaatttca tgaaacgaat ccagcaagaa tacggcctca gctatttatt
     1861 catttcacat gatttaggtg tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa
     1921 aggtcgattt gtagaaatcg gtacaagaga agacatttat aatgacccgc gacatatcta
     1981 tacgaaacgt ctgctttcag ctattccgca aatcgatgta gaaaataggg aaagccacaa
     2041 agaaaatcga cgaagagtag aacaagagta tatccaaaac caaaaagaat actatgatgc
     2101 gactggtcga gtctacgatt tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga
     2161 tggaggtgca agctaatgtg gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa
     2221 gttattattt taagtgttct gatcttctta ttagcccaag cgatgccggg ggatccattt
     2281 accggtttga tcaatccaaa tcaggatccg gcagtgatcg aacagatgcg acaagcagct
     2341 ggcttgaatg acccatggta tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc
     2401 gattttggtc aaagtttctt atacaaacgc tctgttgctg acttgatcgg cgaacggatc
     2461 atcaacactt tatatctatc gatcttgaca gttgtcctta cttatttgat tgctgtgcct
     2521 ctggggatgc ttgctggccg gtatcaaaat tcaatcttgg ataagattgt cgtaatctac
     2581 aacttcttta gttttgctgt tcctttattt atcttcggct tgatcatgct atttgttttt
     2641 ggttatcgtt taggctggtt cccaacaagt ggatcacaaa cactaggttt gcaaggtgga
     2701 tttatcactc aatggataga ccgtttgaaa tatattctgt taccatcaat tacccaagca
     2761 tttttggcga ctgccgtcac aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg
     2821 ctcgattttg tcagaacagc ccgttccaaa ggggtaccga cgaataaagt attcagtcga
     2881 catattttcc gaaatgcagc gttgccgatg gcttctcaaa tggggtatga aatcacttcg
     2941 ttgattgctg gttctgtagt tatcgaaaag atttttggtt atccgggagt cggaaaacta
     3001 tttattgatt ccatcggcca acgggactat accgtgatca catccttagt attgatttta
     3061 ggaatcgcga cgctagtggg gacattactt tcagatatca tcatgagtat cgttgacccg
     3121 cgtattcgga ttcaataaaa ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca
     3181 atacagcagt tcaagaaagt attcctccta tggggatacg aatgattgct cgagaattta
     3241 aaaaagataa aattgctatt ttttcattag gtttgctagt ggttttatta ttagtgatct
     3301 ttatcggtgc gatgctgacc gatcaagaca aagtgatgta tgttagtatc ttcgacaaat
     3361 acgctgcacc aggaacagtt tcgacacaag gacaaaagtt cattctggga gcagatgaag
     3421 gcggacgtga tgtattagga caattgatca taggtgctag aaactctgtt acgatcggat
     3481 ttgcgatcac gatccttact tcgatcatcg gggttggatt agggatcatc tctggctact
     3541 acggagggat cattgacaac atcttgatgc ggatcgttga ttttatcatg attctaccga
     3601 tcatgctgat cattatcgtc ttcgtatcga ttatttccag atacaatgtc tggtccttta
     3661 tattcatcat gagtgcattt tattgggtag ctaaagcccg attattccga agcaagacat
     3721 tatctgaagc aagaagagat tatgtcagtg cctcaaaaac aatgggaacc aatgatttta
     3781 aaatcatgtt ccgcgaaatc atgccgaatc taagttcatt aatcattact aatttgacga
     3841 tgaactttgc ggcaaacatc ggtatcgaga caactttgac tttcttaggt ttcggcttgc
     3901 cggcaaatgt acctagttta ggtacattga tcggttatgc aagtaacgga gaagtcttag
     3961 tgaatcggca atggatctgg ttacccgcat caatccttat tttagttttg atgttgagta
     4021 tcaattatgt tggacaagca ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt
     4081 atagaagcga agggggaaat aggaatgaag aaaacgattt ttggatttgc agcgctgatg
     4141 tctgctgtcg ttcttgcagc atgcgggggc ggaggtacag atacctctgg aaacaactct
     4201 ggaggcaacg gcaataacaa agaggaatta acttttccaa ttgcaacaaa aaataatgat
     4261 gatgcaatcg aagacggcga attagaagct gcggtcgcaa cggacagtca gtttaaagga
     4321 ttgttccagt gggagtttta ccaagattca tatgatgcag cttttatggc tccttctcat
     4381 gaaccgctat ttgcaaacga tgacgatttc agaatcacag acgacggagc tgcaacgctt
     4441 gatctagatg aagatacgaa aaaagcgaca atcaaagtaa aagacaacgt caaatggtca
     4501 gatggtgaag atgtaacggc ggacgatgtg attttccctt acgaaatcat tgggaataaa
     4561 gactacactg gtatccgtta tgacgacact ttccgtaaca tcgtaggtat ggaagaatat
     4621 catgatggaa gtgccgacac aatctccgga atcaaaaaaa tagacgacaa aacagttgaa
     4681 atcgaatata aagaagtaaa cccaagcatg cttcaatcgg gtggtggtat ctggtcgtat
     4741 gcagcaccaa agcatacgtt agaaggtgta gcaatcaaag atatggaatc aagcgatcaa
     4801 gtacgtaaaa atccagtgac ttttggccct tattacatga gtaatatcgt tgcaggagaa
     4861 tcagtagaat acttgccaaa cgagcactac tggaatggta cgcctaaatt gaaaaagatc
     4921 acgatgaaat ctgtaccgat ggcaagtgca actgaagcat tgaactctaa attatatgat
     4981 atgattttcc aaatgccaac agatacttac gatacctata aagaaattgc tggctataca
     5041 aacttaggcc gtgaacaaac ttcttatact tatctaggat tcaagttagg taaatggaat
     5101 gcagatacaa agagtgtgga atacaatcca gattcaaaaa tggctgataa atcattacgt
     5161 caagcaatgg gctatgcact agataatgat gcagtaggtg aacgtttcta tgcaggtttg
     5221 agaagcaatg cgacttcatt gattcctcca gtattccagt cataccatga tgatgagatg
     5281 aaaggattca cacaagatct agacaaagcg aaaaaactat tagatgatgc tggctacaaa
     5341 gatgtggatg acgatggttt aagagaagac aaagatggaa ataaactaac aatcaacttt
     5401 gcttcgatgt ccggtgggga aacagcgcaa ccattggcag attactatat ccaacaatgg
     5461 aaagaaattg gtttggatgt ccaatacaca acaggtcgtc tgatcgaatt caattctttc
     5521 tatgataaat tagaaaacga tgatcctgaa atcgatgttt accaagctgc ttggaataca
     5581 ggaactgatc caaatccaag tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt
     5641 tatgaatcag aagaaaatac aaaattgatc aacgatatca gttcaagcaa agcatttgat
     5701 gaagagtacc aaaaagaagc atatcaaaaa tggcaacaat atgcgtttga agaagcattt
     5761 gcgattccaa cgttgttccg taatgaagta ttgccagtca acgaccgtgt caaagactgg
     5821 gattggtctt atgatgtacc aaacccatgg gcaaatgttt ctgttacagc agattcacgt
     5881 tcataatcac taaaaatgac cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt
     5941 tctgtttatt tctctaatat tcaggtcaag gcgctgtttc catgacttct ccaataaacg
     6001 gtgttcaaag tccagtttct cggtattaag tcaaatttat gcaaaaacat ggaaagtcat
     6061 ttttacaaaa ttttttcttc atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa
     6121 tcactaggat tattaaagct ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa
     6181 aaccagtata ataaggaagt atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca
     6241 accagctgac aaaagaacta aaagaaaact ataacatcgt ttttcacgat ctaaatttac
     6301 ttgaacaagc ttttacccat tcatcctatg tgaatgagca tcgcaatctg caattatcgg
     6361 ataatgagcg acttgaattt ttaggagatg ccgttttaga actgatggtt tctcagtatt
     6421 tattccgttt atatcctcat ttgccagaag gaaaattgac aaaaacacga gctgcgatcg
     6481 ttcgcgaaga cagcttgtcg aaatttgcca aagaatgtca ctttgatcaa tatgttctgt
     6541 tgggcaaggg agaagagaat tcaggaggac gtacgcgacc agcattgctt tgcgacctat
     6601 tcgaagcttt tttaggcgcg ttatatcttg accagggatt cgacacgacc catgcattca
     6661 tcgaaaaagt gatttttcct aaagtcaaag caggtgcttt ttcacatgag atggatcata
     6721 aaacaaaact acaagaagtc ttgcaaaaat caggtgatgt atcaatcgaa taccgattga
     6781 tcaatgaaga aggtccagct catgaacgag tattttggat cgaagtttat gtagatgatc
     6841 aattgattgg cactggacaa ggaaaatcga aaaaattagc agaacaagct gcggcggaaa
     6901 atgcattggc agcactgtaa aagtaagtga gggaaagctt tgtatttgaa acgaattgaa
     6961 atagccggtt ttaaatcatt tgcagacaag acagtcattg attttgaaaa tagcgtcaca
     7021 gcagtggttg gaccaaatgg cagtggaaaa agcaatatta cagaagctat ccgctgggta
     7081 ttaggagaac aatctgctaa aagtctgcgg ggcggaaaaa tgccggatat catctttgcc
     7141 ggttccgata cccgtaagca gctgaatatt gcagaagtca cagtgatttt agacaatagt
     7201 gatcattatt tgccattgga gtacaatgag atcagtgtaa cccgccgtta tcgacggact
     7261 ggggaaagcg agtttttcat caataaacaa agctgcagat taaaagatat ccaagaattg
     7321 ttcttagatt ctggattagg aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa
     7381 gcgattttca gtagtaaacc ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc
     7441 ttgaaataca agcagcggaa aaagaaagca gaacagaagc tttttgaaac agaagacaac
     7501 ctcagccgcg tccaagacat catccacgaa ttggaagaac agttaactcc tttggccgcc
     7561 cagagtgagg cagcaaagga gtttttacgg ctaaaagaaa cactgactca aaccgatgtc
     7621 tctttgatgg ttgccgaaat caagacagca aaaaaagatt gggacaacaa acaagcacag
     7681 cttgcgaaat tcaatctaga attaggcaaa ctatctgagt cgatccaaga acaagaatcc
     7741 attttggcga agcagcgtaa agaaaatgcg caagccgacc gtctgatcga aaaaaatcag
     7801 caagtattat tagatctttc ggaaaaactg aagcagacag aaggacaaaa agatgttttg
     7861 caggagcgga caaagcatac acaaaaaagc tcgcaagaat atcaaacttc attagcagaa
     7921 gcacaaaaaa aggttaaaca ttttgaaaag cttcaagaaa gcttgttgaa agaagcagca
     7981 gaaaaagaaa cagagatcca aaaagctgaa gcaaatctca taaaaacaca gcaagaatta
     8041 gaaaaatacc aaaaatcaac gaaagaatta ttggcagaat taagagatca atatgttgat
     8101 ttgatgcaag aacaagcagc tgtaggaaac gaactgaagt atctagaacg tcaatatata
     8161 caagaaacag caaaaagcaa gcagacgtta gccaagcaat cggaagtaga agcatcagta
     8221 gaccgattga tcttacaaaa agaagaattg acacaaaagc aggcgcagct aaaatcatct
     8281 ttaactgaaa caaaagagaa gttagaaatg atccaacaaa acgggaaaaa gttccaagaa
     8341 gtattagcaa aagaacagcc taaaatgtat caattgatga atcaagtcca gcagctgcgt
     8401 gctcgtcaga aaagccttca agaaatccaa gaaaactatt ttggttttta ccaaggggta
     8461 cgattggtct tgcagcataa acagcagctt tcgggtattg tcggagcagt ggctgaactg
     8521 atcgatgttc ctgcagattt cacgttagct atcgaaacag ctttaggtgg ggcagcccag
     8581 catgtcatcg ttgaaaatga aaaagatgct cggcaagcca tcacgtattt gaaacagcaa
     8641 agaggaggaa gagcaacatt ccttccactg acgacaatca aaccaagaca attaccggct
     8701 catatattga cacaagcagc agctgtagaa gggtttatag ggattgccag tgaacaagtc
     8761 agttatcccg atcaaatcca aactgtggtt caaaacctgc taggaacgat ccttttagcc
     8821 aaagacttaa ctagtgccaa tgcaatcgcg caaacgatcc actatcaata tcgagtggtt
     8881 tccttagaag gtgatgtgat gaatgccgga ggatccatga caggtggagc gaataaacga
     8941 ggaaaccaag gcagtttgtt tgtacaaaat caagaattga aacagttgac tagtgaattt
     9001 gaagaagcag ataaacaact ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa
     9061 acagctcgtt tagccgaaga acaagaagtt ttgcgtacac gaggcgaaca attacgtttt
     9121 gaagaacaag aagcgacaaa tcagctgcaa aacatcataa atgaacttga acgttttgaa
     9181 aaagaaaagc aaatctcaac ttttgagaca cgtgaactgc agcagtttat cgaagattac
     9241 gaaaagcaaa cgaacgaatt gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa
     9301 atcgacgaag aaatcaaatc gttaagtcaa gaaagcgacc agatggaagc cagaagagca
     9361 caagtacaaa gtcaaaaagc acaagaacaa gcagatcttg ctgtcttgaa agagcagttt
     9421 aatcatctgc agatccagct ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt
     9481 caagaagcga tagaaaagca gttggcgaca ttgacagcag acttttctga tcatgaagtc
     9541 acagaagaaa gtttggaaaa acaaattaac gaattatctg ctcagcgaga aacattaaaa
     9601 gccgagttgg caaaagccaa agagcagcga gatagaaggc aaaaggagat cgataagctt
     9661 gaagcagtct tggcagagcg taatcgtgag caaaaagcac gactgtcgga acaatccaaa
     9721 ttagaagtcc aaaaagaccg agctgaaatg attttagata atcatttgac ctatctgcag
     9781 tcagaatatc agctgacatt tgagaaagct tcccaagact atcaagaaac aaccgatatc
     9841 gaagacagta gaacaaaggt ttcatcctta aaagaacaga ttgaaaaact gggaccagta
     9901 aacttaaatt caatcgaaca atacgaacaa gtcagtgaaa gacatacatt cttagcaaca
     9961 cagcgagatg atctgttagc agcgaaaaac caattgtttg aaacgatgga tgagatggat
    10021 gatgaagtgc ggacaagatt caaagaagta tttgaagcta tccgtcaaga attcaaaatt
    10081 gtctttccaa atatgtttgg cggcggtaaa gctgaattag tactgacaga accaactgat
    10141 ttgctgaaaa caggtattga gattgaagta caacctcctg gcaaaaaact gcaaagtcta
    10201 agtttgcttt ctggaggaga acgagcactt acagccatcg cgctgctctt ttctattatc
    10261 cgtgtctgtt ctgttccgtt ttgcatctta gatgaggtag aagcagcgtt agatgaagca
    10321 aatgtgaaac gttttggtcg ttacttgagt gatttccaag atgacacaca atttatcgta
    10381 gtcacacacc gcaaaggaac gatggtcgca gcagatgtac tgtacggagt gacgatgcag
    10441 gaatccggtg tatcaaaaat cgtttctgta cggatggaag atatcaatga agaggcgaaa
    10501 atcaacgaaa cgaaagtgta ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg
    10561 gtacattatt aaatgaacaa aagctgattt cagatgagaa taaacaagca ttagcacaag
    10621 caaaacagca aggggtcaag atcgttctat gtacaggacg ccccttagct gcgatggcgc
    10681 attatctgca agaattagga ctggtagatg aaggtgacta cagcatcact ttcaacggcg
    10741 gtttggtaca aaaaaatgat actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag
    10801 atatccaacg cttatacaaa ctggctcagc aattggatct gccactagat gttttgtctg
    10861 acaatgtggt tttacagctg cctagtgccc caaggaagca atccttgtac aatgtgttga
    10921 ataagctgct gcaattccaa ccagcggata taagcgatat tacagatgaa tttgtgctta
    10981 ataaggcagt aatcgcttat gaccaagaag aattagatcc taaaatcaaa gaaataccag
    11041 cagaattcca tgaaatgtat gagatcatca aaacgagaag tatgcttttg gaatttatgc
    11101 caaaaggtgt tacaaaagct tatggtattt ctttattggc aaaagatctt ggtttgaaac
    11161 aagaagaaat catggcaatc ggtgatgaag aaaatgattt accgatgatc caatacgctg
    11221 gactaggcgt agcgatggag aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga
    11281 cagccagcaa tgaagaaaat ggcgtagcca aagtgatcca aaaatttgta ttagagtagg
    11341 ggggagtcaa atgggatttt ttgataaaat caagcgtgct tttactggcg aagagaaaac
    11401 agaagaagtt gaagtacagg agaagtacga caagggttta gaaaagacgc gtaaaacatt
    11461 tggtgaacgg atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag acttttttga
    11521 agaattagaa gaaacactta tcggtgcaga tgtaggtttt gaaacagcga tacgaatcac
    11581 cgaagcatta cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa
    11641 tgcgatcatc gagaaacttg tggatctcta tgaagcggaa ggaatcaatg aagtaaatga
    11701 actaaatatc caagaagacg gactaacagt tattttgttt gtcggtgtta atggtgtagg
    11761 gaaaacaaca agtattggta aacttgccca tcagttcaaa aacgaaggta aaaaagtatt
    11821 gatggcagca gcagatacct tccgcgctgg agcaatcgac cagttagttg tctggggcga
    11881 acgcgctggt gttgaagttg ttcgtggcaa tgcaggcggc gatccagcag ctgtcgtatt
    11941 cgatgctttg gaaagagcaa agacagagca ggcagatgtt ttactagttg atactgcagg
    12001 ccgactgcaa aataaagtga acttgatgaa cgaattagaa aaaataaaac gagtaatcca
    12061 gcgggaatta ccagaagcac cccaagaaac acttcttgta ctagatgcaa caactggtca
    12121 aaacgcgatg gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt
    12181 gacaaaatta gatggtacag caaagggcgg tatcgtactt gctatccgta atgaattgca
    12241 tttaccagtg aaattggtcg gtcttggtga aggaatcgat gacttggagc cgtttgatcc
    12301 aaatgatttt gtcgttggat tgttcaaagg attactaaaa gaagaataac ccatgtctaa
    12361 aaagcaggag ctgtgacaaa agttgcagct ccttgtttga atgattgttt ttaacatagc
    12421 tgtgtttttg atgccttcat ttttatactt atttgtaagc ggttctcaaa caatttcgtt
    12481 ctttttctta gactttttga ttataattag attcatatga attggaggtc acttaatgat
    12541 gaattttaag caattattat tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt
    12601 tggtatagaa cgggaaggac agcgggttga tcttgcagga aatctagcaa aaaccgatca
    12661 tccagcaatc tttggcgatc gatcctatca cccctatatc caaacagatt ttagcgaaac
    12721 acaaacagag atgatcaccc ctgttaccga ttctattccc gaattatttc agtatctcgc
    12781 tgctgtttat gatgtgactg ctcgttctat accgaaagaa gagatgatct ggccattaag
    12841 tatgccacct gccttaccgg aaaaagacga agagatcatt attgcaaaat taaaaaattt
    12901 cgaagatgtc ttgtatcgac gttatttagc aaaagaatac gggaagcgaa aacagatggt
    12961 aagcggtatc catttcaatt ttgaatttgg cgatgagttg ctaagaacat tgttcagcca
    13021 tcaggaagaa tttcaagatt tttccgaatt caaaacagaa ctttatttga aaacagggag
    13081 aaactttatg cgctatcgct ggatgatcac ttatttattc ggtgcttcac caatgagtga
    13141 gaagaactac tttttagatg aatcacatcc gcaagaacct gttcgcagta ttcgcaacag
    13201 tgcgctgggc tatacgaatc atccaaatgt gaaagtttcc tatgcttcta tgaaacagta
    13261 tttagcagat atcgagcgaa tgatcgaaga agggaaactt tcggaggaga aagaatttta
    13321 tacaccgctt cgtttccgag gtggaaagaa agtcgcagat ttagcaacaa caggtgttcg
    13381 ttatattgaa ttgcggaata tcgatttgaa tccttatgca agattgggta tcaatccaga
    13441 gcaagttcgg ttcttacaat tattcctgat gtacatgtta tggacagaag aaaaagaaga
    13501 ctgtgaccaa tgggtagcag aaggtacgac tcgaaataac aaagtagcac tagaacaacc
    13561 atcagatcaa acagaatttc atcaagaagg tagagaaatt cttgaaggga tgaagcaaat
    13621 gctggttgaa ttggattggt tggattctct ttatttagta gaggaagcat tgactcaaat
    13681 ggatcatcct gaacaaacgt tagcagcgaa actctatcaa gaagcacagc tatcgagtca
    13741 gcaagaagtt gctgtggcat tgggacatca atattataaa gaaagccatg aacgtcccta
    13801 tcagttggcc ggttttcgcg aaatggaact ttctactcag atatttatgt tcgatgccat
    13861 ccaaaaaggt gtccaagtca aagtattaga tgagtcggat caatttttgc gtctgcaatt
    13921 ccaagaccat gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt
    13981 cccacttatc atggaaaaca aaacagtaac aaaaaaagtc ttaaaagaag ctgggttccg
    14041 ggtaccagga ggcgcagaat tttcatcaat ggaagaagcc gtaaaagctt atccgagatt
    14101 tgccgatcag gcatttgtga tcaaacctaa atcaaccaat tacgggttag gtatcacgat
    14161 tttcaaagaa ggagctagtc tggaagatta tcaagcaggt ttagccattg cttttcgtga
    14221 agacagttcg gttttagtag aagaatttat gccgggaacg gaatatcgtt tctttgtgat
    14281 cgacggagaa gtccaagcca tcatgttgcg agtccctgct aatgttattg gtgacggtat
    14341 ccgaacagtg aaagaactag ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca
    14401 tcgtgcacct ttagaattga tccaattagg tgaattagaa caattgatgt tgaaagaaca
    14461 agggttaacg atcgaatcag ttccccaagc caatcaaatt gtttatctaa gggagaactc
    14521 aaatatcagt acgggcggtg attcgatcga tatgactgat gaattttctg aagcttacaa
    14581 aaaaatcgcc gtttctgcgg tagaagcact aggagctaag atcagtggta tcgatttgat
    14641 tataccagat aaagaaatcg atcctacaac tgataaaaaa gcatatggga tcatcgaagc
    14701 aaatttcaat ccagcgatgc atatgcatgt ttatcccttt gcaggaaaag gcagaagatt
    14761 gacgatgaat gtattgaaat tgttatatcc tgaagtattt taaaagaaaa agcttttgac
    14821 agattgtcct atttgatgtc aatctgatca aaagcttttt ttgcatgtaa aaatttatgt
    14881 gttttttcgt tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt
    14941 tcagctggcg aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt
    15001 actgcttctt ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg
    15061 ttgactacag taaatcggct attgtttttc gttgctagct ggatatattg gttttggaca
    15121 gaaaggggta gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt
    15181 aaaaagtttt ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag
    15241 cgttccctga atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca
    15301 taaagtccct tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt
    15361 cttctctttg tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa
    15421 aagcggttgc ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag
    15481 aaaggtggat tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat
    15541 ttcattgttc atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca
    15601 aagcttacaa atcttgctag cgactcgttt gccacgaacg atttgtttga tccttgtggg
    15661 agcgaccagt agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc
    15721 accaacgact gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt
    15781 cctacctggt gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg
    15841 aacattgctt tttttatccc ttacgcgatt gttcccccag aaaaatcaat tgatgcttcc
    15901 gttgattgga gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca
    15961 attccagttg gtacaaaata tgacttcatg gcttcaaggg aattttgcga ctgtgatacg
    16021 aggaaattat gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc
    16081 ttaccgattt acgatcattg gtctgggcga atcgtttgca gtaaatttgg gaatagatta
    16141 ccagaagatg cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat
    16201 catggtagga agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata
    16261 tggagatcaa gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt
    16321 attttgcgat cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat
    16381 cgttggaatc atcggtgggg ctatcttcct ttatcttctg ataagggggc gacgatatgc
    16441 ttaaaaaaaa gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa
    16501 tgtacttgac gatccataca tatggaaact ggcattttgc atgggcattg aggagtaaga
    16561 aaatttttgc atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga
    16621 caattacgca taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc
    16681 ttgtccaaac ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta
    16741 caggtttttt cttattgaac gttgggctga tggtcgggtt gagtctgttg ttatttttct
    16801 ttcttttgaa aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag
    16861 gtacgctttt tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg
    16921 atttgcttca aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc
    16981 ctgcttcttt gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag
    17041 acgttttcca tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg
    17101 aatttttgat tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc
    17161 cagttacttt tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc
    17221 gtcatggtca gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac
    17281 agctcgcagt agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg
    17341 tcggaggaat ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga
    17401 tttacaaaag gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac
    17461 gattcctgaa aagaagttga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt
    17521 gttgtcgatg atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa
    17581 cgaagtaaag gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc
    17641 gaatgaaata aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta
    17701 tacgaaagga aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct
    17761 tgggttagaa gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg
    17821 tgcgtatatt gcaatggtac tagcgcaaga cacggattat attttactgg atgaaccttt
    17881 gaacaatcta gatatgaatt ttgcggttca aatcatgcag atcttaaaaa aattagtaca
    17941 agaaatcggg aaaacaattg ttatcgttct tcatgatatc aactttgctg caagttatgc
    18001 agacgaaatt gttgcgatga aagatggaca gttattcacg caaggaacga cccaagaaat
    18061 gataaaaaaa gatgtattgg atgaattata cgaaatggac atacggattt gtgagattga
    18121 aggccgcaga ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat
    18181 tagtagtagg tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagcaaca
    18241 acaccagtca ggcagataca aagaaaagca gtacgcaagc tgaaacggca ttaacgataa
    18301 aagacagtaa tggcgatcaa atcgaggtgc caaagaatcc tgaaaaagtc gtcgtattcg
    18361 acaatggttc tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag
    18421 ctacgagcag ccttcctgag tatctttcct cctataaaaa agtggaatca gcaggtggga
    18481 tcaaagaacc agacttagaa aaaatcaatc agcttcagcc aaacctgatc atcatttctg
    18541 gccggcaaag agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt
    18601 tagatgcgaa aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct
    18661 ttggtaagca agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga
    18721 cgaaaaagaa agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc
    18781 aattatcagc ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg
    18841 aacaagcaga tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg
    18901 tgctggaaaa aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag
    18961 acgacagcaa agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa
    19021 atcagcagat catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat
    19081 caatgaaact gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga
    19141 ttaaactctc atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat
    19201 cctcaaagaa gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt
    19261 ttatttattt gggaaatctt ttttcaacgt gaattcattc tctacatcat acgggttgat
    19321 tctagaatgg ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc
    19381 ctttgatttg aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt
    19441 tattttgtac cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc
    19501 cactagacta gaaaatatga gaagctattt tctgtccagc gttcttatta cttaaagcaa
    19561 atcgtagttg tcaaaatctc ttctaaacga tgttccatca gctgaccttc ggtattagct
    19621 caaagcactg atttcacaac ctctatttag gcatgtgtct cagcaagcgg ttgatagttg
    19681 atgatctgca tttttcgata aaaatcggcg ggaagcttac tccaccgttt taataattga
    19741 tagaaataac tttcttctgc tttgatttta tactcgctta atatcaatcc aaaactattc
    19801 gttttttttg aagctaattt tttataaata tccaatgctt tggttttatc ttcattgaca
    19861 aagagtgttt ttacttcaat ataagaaccc tcgatatttt gtgtttcttc gataaaatag
    19921 atgtttttta tatattcctt catagtgatt cctccaaaga agtcttttat ttgataagtt
    19981 tactctaccg aagagttgtt aataatccgt gaacaaaaag taattctttt tccaatagtt
    20041 gttaaaggtt ttttattttt tatagtattt tttgttattt aagaaaggaa taaggttaaa
    20101 caaataacag gttgcttttt gggtcttttt ccgttcaaga aactgaacga agaaaaggga
    20161 aagttgtctc ttttgtgata agagaaatca taataaacga atgacttact ttccaataaa
    20221 ttgagaacag tcagaaccag acaaattagt tgcatctgat gaataaagtg attattctat
    20281 gacatgaggt gatacagatg aaattagaag tgacaaaaga agcacaagaa gtattgaaga
    20341 ataaattaga agcagacgat caacttttat tagatatcga aaacggtgac ggtccatttg
    20401 cagaaagcca agtcagttgt caattagaca cggcatttcg attgatcatt gttaaaaaag
    20461 atacacaaac agatctaagt ctttattctg tagtggctga tacaccagtt ggaccaatca
    20521 aaatcaaaga cagtgcaata ctttacatgg atgatccatc cacacttgct ttagaaccaa
    20581 cttataatag tttacaatta aaaggaccaa gtggcttgag aaaaggcaac ttgcaagttg
    20641 ttcgaaaaga ctagagggat aaaatgggaa atttggcaat catcagcgac ctacatgtgg
    20701 acatcaataa attcggcgaa ccagagctaa tgcagctttg gcaagttttg caaaagcaca
    20761 aaatcactcg gcttcatcta gccggagata cggctaataa agtagatcgc tgcctagcgg
    20821 ttgtcgaatt tttcgccaat aaaggattat ctacgacatt tcatttcggc aatcacgagt
    20881 tggcagatgt gcccaatgaa cagatgatgg accattttcc agacccacat tttttaaatg
    20941 aacggtatat tgctttaaat gagcagaccg ttcttttagg gatgaatggc tggtacgact
    21001 atcagttttc cgaattgaaa gattacgagg caatcgtacg aatgaagcgt ttgtattggt
    21061 atgatcggtt catccagcgt gggaaaacag atctcgaggt caatgcagaa atgcttgcag
    21121 ctgcaaaaaa tttgctagac cagctggaag caaaaaaact tgaggtgatt ttatccactc
    21181 atttcgttcc taaacgggca tttatcatgt atcaatctgc tccttatgag cgttggaaca
    21241 aattaaatgc atttttagga tcagaatcat tcggagaatt acttgaccgt tattcaaata
    21301 tcaaacaagt agtttttggc catacgcatc gtcgctttga agataaatcc atcggtcaca
    21361 cgatttacag ctgcagacct tttggttatt attatgaatg gcagctgacc cgagattttg
    21421 tcttgaaaga acagttgcta gacagctatg atcctatgaa attacgtgtc ctcttgcgtg
    21481 cccatcaccc agcttttcag gcctatcaaa cacaacattt gcaagaggaa tttgaacaag
    21541 cgatgacaat cgttccgtat tgagacagca ttagtaatgt gtaaaagaaa ataccttgta
    21601 aaacataagg ataagtaaga gcagtagatc catttatcgt ggatatgctg ctttttttgt
    21661 ggaaaataat cagcgaatgt ttatttgatc ggatgatttg taactcgcag agtactagat
    21721 gcaagcgttt attctttttt tgaacgataa tcacaaaaaa aaatataata tacatttttt
    21781 atttaattat aattaatatt gattgaagtc taggcagtca agtcacattt tgatagatag
    21841 gagggggact tacgaattat tttaaaggct atgacagctg tatttctaga ttgtgtttgc
    21901 atcaagttgc aataaaaact agaaatcaaa tcgggctgat aatcatttat aactaatcga
    21961 tacataggac caaatatagg tctatgttct tttatttcat aagtatcgta aaacagaatt
    22021 ttgctatgga ttaatccaat cgactgatat gtttagtaaa taaagaaaga ggaatgtcaa
    22081 tgatgaaaaa agaaatcgta ttaacagcag caacgatgtt attcagcatg gtagcaagta
    22141 cgacttttgt ttctgcaact gaagtatacc caaaggaata taacacagaa ggaacgatta
    22201 catttgaagc aggagatgag ggtgtaactc ctcctgttga tccagagaat cctgatccca
    22261 ataaacctgt cgatccatca gatcctccaa gccctggacc aggaggggca ttatcgattg
    22321 attatggatc aaaattcaaa tttgggactc aaaaaatttc tactgcggat aaaacgtatt
    22381 atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc gacatatgtc caagtgacag
    22441 ataggagagg taccttatct gggtggaaat taagtgtctc tcaaccagaa cagtttaaaa
    22501 ctgccagtgg ggatgaacta gttggagcac agctgaaatt tacgaaaggg caggctgttt
    22561 ctttggttga tccaacatat atgccgcaaa cggtgaattc tgaattaacg ttgacaccgg
    22621 gaggaaacaa tacgttagca attaatgcta aatcaggaac aggtgtgggg acttgggtct
    22681 atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt acagctttcg gtacccggta
    22741 aatctgtcaa gttagcgcaa caatattcca ctaaattagt ttggacatta gaagatacac
    22801 ctaacaacta gtttactgaa aagaactgtt aaaaataaat acgtagtcat attgtcttgc
    22861 gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt tgtttggata cgaatagttc
    22921 atctaggaat ttaacgatca ggaggaaaag atatattgaa aaagaggagg atcgtgctca
    22981 ttttaagcag tctcttagtg actaatatct ttccgatcat agggtactct ttggaattga
    23041 atcagttgga gcatggaaat agaagagacg aagaaagcaa actcacttcg acattcgaca
    23101 gtttaataga atccaaccag acacaagaaa gcagtgagga tttaagtatt tcgagtagtg
    23161 gagagaccac tgacaacgta gaagctgagg gttcggacaa acaaggactg gaagatgcac
    23221 caatttctgt ggttgagctg cctgacgatc ccaatattat ttctatcgat aaaatttttc
    23281 aaacacctat cggtactagc acgtccatat tggaaaatgg gaagttactg cagttgaacc
    23341 cagcagaaag gtctcaaaga ggagctattt ggtcaaataa gaagattagt ttactctcgg
    23401 attttacatt taaatcttat ttatacttag gaaatcaacg agatagagcg ggagatggca
    23461 tgacctttac attgactggc gatcctagaa tggctagtaa tcccagtcaa gttattggat
    23521 cgccaggtat gggaattggc tcatattcta ctcgttctgg ccaaccttac attgcgaatg
    23581 cgctctcgat tgagtttgat acgtataaaa atagtggttc ttctgatcga atggatcgag
    23641 agataactgc tgataataga gggcatggac atgtcgcttt tgtcacgcct aaaccaaata
    23701 ataataatta ttctggagag catactggcg tgacggtagc tcctacattt ctttctaatg
    23761 gcacatggcg cttgttaata attaaatggg acgcggagca aagaagtctt acttatgatt
    23821 tggaaggtgt cggtcgctct tctcggatta tacctgatct cattaggcag tttggttcta
    23881 cagaagtaaa ttgggggttt acttcttcaa caggtacttt ttttcaagaa aatgctgtcg
    23941 ctgtcacgcg acttccatcg aatgtcacat caacagctga tgtaaaagtc aacgatagtg
    24001 attatggggc catcgctgaa gctggaggaa acgataagat aactatcaga aatacattga
    24061 aggtcgaggg ggattttctt gaatataaga aaatgcagac tagtatcgac ctcccacaag
    24121 aattaactat tcccgagcat atcttgatca atggaaatca aattgatgcc aaagatatct
    24181 tgataaccgg aaatcgagtc acgctaaatc tgacagatta ttttattgct ggagatacgt
    24241 taataatcga attggagaca actttgcagg aagtaattcc tgaaaaagta ctcactatgc
    24301 attttgaata ccttgaaaac gagaatttga ttgagcaatc gaatgaaata aacctaaaga
    24361 tcgcaaagct gaaggagata gatatccatg tttattataa agataaagac agtcagattg
    24421 atattgctga aatgaaaaca atcactggga aaataggaga gaagtataaa gaaactccta
    24481 ttgatgttga agggtatgtc tttgactcgg attcagcgaa tacagaaggc atcttctccg
    24541 aaaatacaga ggatatctat ttttattaca gaaaaggcaa actttatctc tcaactgctc
    24601 ctcaatatgt caattttgga aaacataaaa tatccagtag accattaagt gaattcggac
    24661 agttttcagg tgggttgaaa attatagatg aacgggcttt tggaagttgg aaattacaat
    24721 tgaaacaaga gaaagttctt acaaatggag attttgagct tccagaagct ctttcctttg
    24781 ttacctctga aaatacaacg gttataggga catccgctgt tacgattttc gaaagtgatc
    24841 aaaagggtga aagcgatttg tcctctcttt tagattctac agctcatcgt ggaatcagaa
    24901 tagatattcc tgtagaaaac caaagaatcg gaacctttga ggggaaacta tcttggatac
    24961 ttgcagatgt cccaggaaat taacaggaag agaagaatat aacaaaatta ttgagtttca
    25021 agaagggcac aggtaccata ggctcttgtg tcccacagag acaagatttt gttatagtct
    25081 tctcttacat atgattaaag gagcgatttt cttgagacca aaacaacaat tatttataac
    25141 actcctagtt attttagggg cactttttaa caatatatca gtgtcagcta gtgcatttaa
    25201 tttttcagtg acgccgataa cttcagagaa tcaaatcgac aagagaaaaa cttacttcga
    25261 tcttcaactt gatccggatc aggaagtaga agtaaaagcg gaattgagaa atgatacaga
    25321 aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc accaattcaa atgtgatggt
    25381 tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg atctttgatc tggtagatta
    25441 tgtgagctat ccacagatag tgacgctaaa acctaaaagt gtccaaaccg ttgcttttca
    25501 tgtcaggatg ccgaataagc gatttgatgg tgtattggct ggtgggatca cattccaaga
    25561 aatccagacg gaaaaggacc aaacagaaac taaggatcaa agcttatcga ttgagaatga
    25621 atacacctat attattgctt tgttgatgcg acagacactg aacgaagttg ctccgaacct
    25681 tactcttcat taagttaaac cagatcaaat caatgcaaga aatgtcatct tagcaaacgt
    25741 tcaaaatgat caaaaaacct atatcaatca agtagttatt gaaacaaaaa ttacaaaaaa
    25801 aggacattct gaagttttat atcaagagga aaaagaagcg ttgcagattg cgccgaatac
    25861 aaatttttct tttccaactg ctttaaatgg tcaaccgcta accccaggtg aatatcacct
    25921 tacaatgact gttttaggaa atgaaaatga aaatggaaaa ttttcgagaa aaaaaggaaa
    25981 tactacgatc aactatacca atcaatgggt atttgaaaaa gattttacaa tagacgaaaa
    26041 agtagccaag gaattgaaca cgacggatgt tacgattaaa aaagacaata attggattta
    26101 tatcttagta gcttcacttt tcttattgtt tattttatta tgcatacttt
//
LOCUS       DACDCJ010000038        25701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDCJ010000038 DACDCJ010000000
VERSION     DACDCJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.038"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IV038_001513"
     CDS             133..3660
                     /locus_tag="IV038_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8341739.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IV038_001514"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IV038_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP8341740.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IV038_001515"
     CDS             7448..7789
                     /locus_tag="IV038_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8341741.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IV038_001516"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IV038_001516"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP8341742.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IV038_001517"
     CDS             9146..11566
                     /locus_tag="IV038_001517"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP8341743.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV038_001518"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IV038_001518"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP8341744.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IV038_001519"
     CDS             13000..13737
                     /locus_tag="IV038_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8341745.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IV038_001520"
     CDS             13751..14566
                     /locus_tag="IV038_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP8341746.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IV038_001521"
     CDS             14579..15226
                     /locus_tag="IV038_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8341747.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IV038_001522"
     CDS             15242..15898
                     /locus_tag="IV038_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8341748.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IV038_001523"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IV038_001523"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP8341749.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IV038_001524"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IV038_001524"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP8341750.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IV038_001525"
     CDS             18240..18758
                     /locus_tag="IV038_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP8341751.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IV038_001526"
     CDS             18865..19365
                     /locus_tag="IV038_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8341752.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IV038_001527"
     CDS             complement(19645..20565)
                     /locus_tag="IV038_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP8341753.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IV038_001528"
     CDS             20730..21743
                     /locus_tag="IV038_001528"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP8341754.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IV038_001529"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IV038_001529"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP8341755.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IV038_001530"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IV038_001530"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP8341756.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IV038_001531"
     CDS             complement(23383..24144)
                     /locus_tag="IV038_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8341757.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IV038_001532"
     CDS             24333..25427
                     /locus_tag="IV038_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8341758.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25701)
                     /locus_tag="IV038_001533"
     CDS             complement(25513..>25701)
                     /locus_tag="IV038_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8341759.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8510 a         4455 c         5416 g         7320 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattacttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat t
//
LOCUS       DACDCJ010000039        25674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDCJ010000039 DACDCJ010000000
VERSION     DACDCJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.039"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            170..514
                     /locus_tag="IV038_001534"
     CDS             170..514
                     /locus_tag="IV038_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP8341760.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IV038_001535"
     CDS             515..934
                     /locus_tag="IV038_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP8341761.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="IV038_001536"
     CDS             complement(1026..1376)
                     /locus_tag="IV038_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341762.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IV038_001537"
     CDS             complement(1342..2673)
                     /locus_tag="IV038_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP8341763.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IV038_001538"
     CDS             2929..3495
                     /locus_tag="IV038_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8341764.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IV038_001539"
     tRNA            3640..3727
                     /locus_tag="IV038_001539"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IV038_001540"
     CDS             4061..5035
                     /locus_tag="IV038_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8341765.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IV038_001541"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IV038_001541"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP8341766.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IV038_001542"
     CDS             complement(7450..7896)
                     /locus_tag="IV038_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP8341767.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IV038_001543"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IV038_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8341768.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IV038_001544"
     CDS             8361..8879
                     /locus_tag="IV038_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8341769.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IV038_001545"
     CDS             complement(8956..9912)
                     /locus_tag="IV038_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8341770.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IV038_001546"
     CDS             10078..10884
                     /locus_tag="IV038_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341771.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IV038_001547"
     CDS             10881..11870
                     /locus_tag="IV038_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8341772.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IV038_001548"
     CDS             11867..12871
                     /locus_tag="IV038_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP8341773.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IV038_001549"
     CDS             13003..13545
                     /locus_tag="IV038_001549"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP8341774.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IV038_001550"
     CDS             13565..14263
                     /locus_tag="IV038_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP8341775.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IV038_001551"
     CDS             14284..14496
                     /locus_tag="IV038_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP8341776.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IV038_001552"
     CDS             14496..15458
                     /locus_tag="IV038_001552"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP8341777.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IV038_001553"
     CDS             15476..15871
                     /locus_tag="IV038_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8341778.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IV038_001554"
     CDS             16034..16399
                     /locus_tag="IV038_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP8341779.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IV038_001555"
     CDS             16396..18207
                     /locus_tag="IV038_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP8341780.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IV038_001556"
     CDS             complement(18269..18436)
                     /locus_tag="IV038_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP8341781.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IV038_001557"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IV038_001557"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP8341782.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IV038_001558"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IV038_001558"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP8341783.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IV038_001559"
     CDS             21193..22173
                     /locus_tag="IV038_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP8341784.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22262..23086
                     /gene="tsaB"
                     /locus_tag="IV038_001560"
     CDS             22262..23086
                     /gene="tsaB"
                     /locus_tag="IV038_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP8341785.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23070..23612
                     /gene="rimI"
                     /locus_tag="IV038_001561"
     CDS             23070..23612
                     /gene="rimI"
                     /locus_tag="IV038_001561"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8341786.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23619..24083
                     /gene="rimI"
                     /locus_tag="IV038_001562"
     CDS             23619..24083
                     /gene="rimI"
                     /locus_tag="IV038_001562"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP8341787.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24080..25096
                     /gene="tsaD"
                     /locus_tag="IV038_001563"
     CDS             24080..25096
                     /gene="tsaD"
                     /locus_tag="IV038_001563"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP8341788.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8328 a         4400 c         5377 g         7569 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa tttgcccgcc agcagataat
    22261 catgtattat tgtactagtt ttgcctttgg tattttcatc ctcagaataa aaaagtatgc
    22321 tacaatggaa ggcgagaatg tcatagcaga aaggatcaaa tatatgatta cattaggaat
    22381 agatactgca aaccaaactt tggcgatcgg tgtggtagaa gacgaacaaa ttcttggaca
    22441 aatacaaaca aatatcaaac gcaaccacag tgtaacactg atgcctgcaa tcgaccaatt
    22501 gtttgcagat cttcaaataa gtccaaaaga cattgaccga atcgcggttt ctgatggtcc
    22561 cggttcttat acagggcttc gtataggtgt aacgacagca aaaacgattg cttatacatt
    22621 agataaagag ctagtaggag tatctagtct gaaaaccatc gcggctaatt gcatcggtgt
    22681 ggataatatt attgtgccca tgtttgatgc tcgaagaaaa aatgtatacg ctggggcata
    22741 ccgctggaaa gaaaatagat tagaaactgt acttgaagat gtacatattt ctgcttctga
    22801 tttgttcaca caactacaaa agctgaatga agctatttat ttcgtcggga gtgattgtca
    22861 caaattcttc tctgaaataa aggaaatatt gcctagtgcg atgattaata cagttccagc
    22921 atgggatatt ccaagtggtg taactgtggc tcaattaggt gctttagttg aaccagttgc
    22981 agagaaacaa gcttttcttc ctcgctattt aaaacgagta gaagcagaag aaaaatggtt
    23041 ggagacgcac acacctggag acgaaagtta tgttgaaaaa atttagagcg tatgccagaa
    23101 gcttttttca agataaaatg atttatacag aaaaaacagt cgaacaaatc gatcgtcgtt
    23161 acgtactaag agaattgact acagaagata tcaaagagct gctggatgtc gaacgagaag
    23221 tctataatgg cgaattgcca tggacaaaaa gtgcctttat atcagaattg aagtcaccgc
    23281 ttcctcatct atatctaggt atctttgata atggaagaat gattggtttt atcggttcaa
    23341 gagcgttcgg tttagattgc catatcacga atattgccgt tcgaccggca tatcagcgta
    23401 aacgaatcgg ttcgctgctt attgacgaaa tcgagaactt tgctataatg aatcgctgtg
    23461 agacgatgtc attagaagtg cgtatgagta atcaagatgc acaaagactt tatcggaaat
    23521 tggggtttca agcaacaacg atcaaaaaaa gatattacac tgaaacaaaa gaagatgcgt
    23581 tggatatgat aaaactttta gaaaatgagt gagattagtt gatttgcaga aaaaaagatt
    23641 ttgatgaagc gtacttggca gaatgcgtat ggatgattag cgaaaatgct tatccacatg
    23701 gttcaccttg gacgaaaatg cagtttttgg cagacatcca acagcctcac tctgattatc
    23761 tgcttttagc tgaagataac cagatctgtg gctttattgg atactcgaaa gtaatagatg
    23821 aagtcgagat cacaaatatt gctgttgcag tttcagaaca aggaaaaggg catgcacggc
    23881 atttgttaca attactgatt acggaacaaa taaaagattc gctgcatgtt tttttagaag
    23941 tacgtctatc taatgaagca gcaagaaaac tttatgaatc agaaaagttt cgtatattag
    24001 gtaaacggaa atcatattat cgcaatccga cggaagatgc gatcattatg agcagaaagt
    24061 taaaaacgga gacgatgaaa tgaatgaaga attgattctg gcgattgaat caagttgtga
    24121 tgaaacgagt gtagcggtcg ttagaaatgg aacagagatc ttatctaaca tcgttgcttc
    24181 tcaaataaac agtcataaac gttttggtgg cgtagtacct gaagtagcta gccgccatca
    24241 cgtagaacaa atcaccttat gcttggaaga tgcactggta gaagctggtg tgtcggcaga
    24301 agatttatcg gcagtcgctg tcacttatgg accaggactt gtcggctcgt tgctgattgg
    24361 tatttctgca gctaaggctt ttgcttgggc acaccaattg ccgcttattc ctgtaaacca
    24421 tatggcaggg catatctatg cagcccgtct ggttaaacca ttccagtttc cattgatggc
    24481 acttcttgtt agcggaggac atacagaatt agtctatatg caagaagatg gatcatatga
    24541 aatcattggg gagacaagag atgacgcagc aggagaagct tatgacaaag ttggaagagt
    24601 attaggtctt tcttacccaa gcggaaaaga aatcgatcag ctagctcatc agggaaaaga
    24661 taattaccat tttccgcgag cgatgatcca tgaagataat tatgatttca gttttagcgg
    24721 attaaaaagt gcatttatca atttggtgca taatgcacaa cagcgtggag aagatctaga
    24781 caaaaatgat ttagcagcta gctttcaagc aagtgtgatc gatgtattga ttactaaaac
    24841 acttcgagct tgtcaaaatt atccagtcaa gcaactgatt atagctggag gtgtagcggc
    24901 caatcaaggg ttgcgtgaag gattgcagga tgctttggca actaaactcc ctgaggtaga
    24961 actagtgatt ccccctcttc gactttgtgg agataatgct gcaatgattg gagcagcagc
    25021 acatgtggaa atgcaaaaaa gaaattttgc tagctatcag ttgaatgctg atccaagctt
    25081 ggttttatct gaataaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
    25141 ccaggaaaaa cggcttctca tgtttttgac ctggctgttc ttattaccgc gtatgcaagc
    25201 ttttgaacgc cgtttaatca agagttcgta agcctgtcgt ttaactctga ttcacaagga
    25261 acaatttcaa aaaacagctc ctcatgttta tgaacattat gacttgtgac cgaagagttg
    25321 acaggtacac accgtttaat cgaaataggg ctgtgacacg tttttgtcaa agccctattt
    25381 tttacatact tttaatcatt attgttattt tgctattttt tacagcttat ttatcattat
    25441 ccgttcctct gattgccctc actgagtaat cgtttattgt cgaggaatat cgaataatct
    25501 agatcggatg aggacaagac gcttcatcta gttaatctaa cagtattttt tgcaaacagt
    25561 gaatattaca taactgaaac acacatagaa aatctttgag tcttgtccag tgatctaaaa
    25621 ctgccggatc tttcagaagt tttagggagg aaaataagca gtgaccctga tatt
//
LOCUS       DACDCJ010000040        24944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDCJ010000040 DACDCJ010000000
VERSION     DACDCJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.040"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IV038_001564"
     tRNA            8..81
                     /locus_tag="IV038_001564"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IV038_001565"
     tRNA            101..173
                     /locus_tag="IV038_001565"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IV038_001566"
     tRNA            201..274
                     /locus_tag="IV038_001566"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IV038_001567"
     tRNA            288..361
                     /locus_tag="IV038_001567"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IV038_001568"
     tRNA            385..474
                     /locus_tag="IV038_001568"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IV038_001569"
     tRNA            487..560
                     /locus_tag="IV038_001569"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IV038_001570"
     tRNA            579..654
                     /locus_tag="IV038_001570"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IV038_001571"
     tRNA            656..726
                     /locus_tag="IV038_001571"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IV038_001572"
     tRNA            740..813
                     /locus_tag="IV038_001572"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IV038_001573"
     tRNA            838..925
                     /locus_tag="IV038_001573"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IV038_001574"
     tRNA            947..1018
                     /locus_tag="IV038_001574"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IV038_001575"
     tRNA            1041..1130
                     /locus_tag="IV038_001575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IV038_001576"
     tRNA            1143..1216
                     /locus_tag="IV038_001576"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IV038_001577"
     tRNA            1235..1307
                     /locus_tag="IV038_001577"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IV038_001578"
     tRNA            1311..1383
                     /locus_tag="IV038_001578"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IV038_001579"
     tRNA            1388..1458
                     /locus_tag="IV038_001579"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IV038_001580"
     tRNA            1471..1544
                     /locus_tag="IV038_001580"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IV038_001581"
     tRNA            1556..1627
                     /locus_tag="IV038_001581"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IV038_001582"
     CDS             1893..3086
                     /locus_tag="IV038_001582"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP8341789.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IV038_001583"
     CDS             3216..4688
                     /locus_tag="IV038_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8341790.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IV038_001584"
     CDS             4771..5559
                     /locus_tag="IV038_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP8341791.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IV038_001585"
     CDS             complement(5626..6294)
                     /locus_tag="IV038_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8341792.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IV038_001586"
     CDS             6409..8121
                     /locus_tag="IV038_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341793.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IV038_001587"
     CDS             8124..9893
                     /locus_tag="IV038_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341794.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IV038_001588"
     CDS             complement(10098..10664)
                     /locus_tag="IV038_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP8341795.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IV038_001589"
     CDS             complement(10664..11620)
                     /locus_tag="IV038_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP8341796.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IV038_001590"
     CDS             complement(11620..12276)
                     /locus_tag="IV038_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP8341797.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IV038_001591"
     CDS             12646..14664
                     /locus_tag="IV038_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8341798.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15091..17505
                     /locus_tag="IV038_001592"
     CDS             15091..17505
                     /locus_tag="IV038_001592"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP8341799.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="IV038_001593"
     CDS             17853..19406
                     /locus_tag="IV038_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP8341800.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="IV038_001594"
     CDS             complement(19787..21571)
                     /locus_tag="IV038_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341801.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="IV038_001595"
     CDS             complement(21586..23307)
                     /locus_tag="IV038_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341802.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="IV038_001596"
     CDS             23407..24168
                     /locus_tag="IV038_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8341803.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7779 a         4517 c         5240 g         7408 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaattccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagtgaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcagcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaa
//
LOCUS       DACDCJ010000041        24729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDCJ010000041 DACDCJ010000000
VERSION     DACDCJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.041"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..869
                     /locus_tag="IV038_001597"
     CDS             <1..869
                     /locus_tag="IV038_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341804.1"
                     /translation="KKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEK
                     SVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIR
                     RELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKF
                     FLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGK
                     PEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQI
                     DDFPEEIIDRIE"
     gene            complement(962..1696)
                     /locus_tag="IV038_001598"
     CDS             complement(962..1696)
                     /locus_tag="IV038_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP8341805.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            2022..2432
                     /locus_tag="IV038_001599"
     CDS             2022..2432
                     /locus_tag="IV038_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP8341806.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            2425..3126
                     /locus_tag="IV038_001600"
     CDS             2425..3126
                     /locus_tag="IV038_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP8341807.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            3126..4739
                     /locus_tag="IV038_001601"
     CDS             3126..4739
                     /locus_tag="IV038_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8341808.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            4729..4950
                     /locus_tag="IV038_001602"
     CDS             4729..4950
                     /locus_tag="IV038_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP8341809.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4947..5708
                     /locus_tag="IV038_001603"
     CDS             4947..5708
                     /locus_tag="IV038_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP8341810.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            6089..6598
                     /locus_tag="IV038_001604"
     CDS             6089..6598
                     /locus_tag="IV038_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP8341811.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(6668..7207)
                     /locus_tag="IV038_001605"
     CDS             complement(6668..7207)
                     /locus_tag="IV038_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP8341812.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVAVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(7347..7958)
                     /gene="rpsD"
                     /locus_tag="IV038_001606"
     CDS             complement(7347..7958)
                     /gene="rpsD"
                     /locus_tag="IV038_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP8341813.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            8274..9071
                     /locus_tag="IV038_001607"
     CDS             8274..9071
                     /locus_tag="IV038_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP8341814.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            9099..9734
                     /locus_tag="IV038_001608"
     CDS             9099..9734
                     /locus_tag="IV038_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP8341815.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            9754..10527
                     /locus_tag="IV038_001609"
     CDS             9754..10527
                     /locus_tag="IV038_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8341816.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEKQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10935..11732
                     /locus_tag="IV038_001610"
     CDS             10935..11732
                     /locus_tag="IV038_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP8341817.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            11710..12123
                     /locus_tag="IV038_001611"
     CDS             11710..12123
                     /locus_tag="IV038_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341818.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            12107..14752
                     /gene="mutS"
                     /locus_tag="IV038_001612"
     CDS             12107..14752
                     /gene="mutS"
                     /locus_tag="IV038_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP8341819.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            14770..16878
                     /gene="mutL"
                     /locus_tag="IV038_001613"
     CDS             14770..16878
                     /gene="mutL"
                     /locus_tag="IV038_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP8341820.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     LQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16900..17460
                     /locus_tag="IV038_001614"
     CDS             16900..17460
                     /locus_tag="IV038_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP8341821.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(17545..19533)
                     /gene="treP"
                     /locus_tag="IV038_001615"
     CDS             complement(17545..19533)
                     /gene="treP"
                     /locus_tag="IV038_001615"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP8341822.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            19756..20469
                     /gene="treR"
                     /locus_tag="IV038_001616"
     CDS             19756..20469
                     /gene="treR"
                     /locus_tag="IV038_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP8341823.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            20546..20992
                     /gene="msrB"
                     /locus_tag="IV038_001617"
     CDS             20546..20992
                     /gene="msrB"
                     /locus_tag="IV038_001617"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP8341824.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            21162..21764
                     /gene="ruvA"
                     /locus_tag="IV038_001618"
     CDS             21162..21764
                     /gene="ruvA"
                     /locus_tag="IV038_001618"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP8341825.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            21777..22778
                     /gene="ruvB"
                     /locus_tag="IV038_001619"
     CDS             21777..22778
                     /gene="ruvB"
                     /locus_tag="IV038_001619"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP8341826.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            22807..23385
                     /locus_tag="IV038_001620"
     CDS             22807..23385
                     /locus_tag="IV038_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP8341827.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            23437..23919
                     /locus_tag="IV038_001621"
     CDS             23437..23919
                     /locus_tag="IV038_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP8341828.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            24036..24410
                     /locus_tag="IV038_001622"
     CDS             24036..24410
                     /locus_tag="IV038_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341829.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         8089 a         4421 c         5278 g         6941 t
ORIGIN      
        1 aaaagaaatt attccacttt ttagcaatca acctgttggc tgaacaagct gattctttag
       61 atattgctag caatcagtcg ctattttttt cggatatagg attttaccac ttaaaaggag
      121 tattaggaga aaaatccgtt atttttatca ataagacact ctcttggctt tatcttcatg
      181 ataaattgga tcaaaaatac ttgagaaaaa atgaagatga aacaatcgaa tggttgaatg
      241 aacgattcat tgaaagtttt gaaaatcagt ttaatctgtt gcgagaagag acgagccaat
      301 tgatccgaag agagttaacc aaaatacatt ttaaactttt gcattacact attaataaag
      361 ctgatgaatt tccacttgat gtttcttttt ttagagaaaa ctatccagca ttttattgct
      421 atttgataga gtatataaat gatctaacgt caaaacgcaa agagttgctt caagataaat
      481 tttttctttt tttcagttat ttattattat tgatcaatca catacccatt caactgattt
      541 ctgaaccagt aaagatcatc atcgattttt cttatggtgc tgcctataat caatttatca
      601 agaaaaattt aagcttgtac attaatctga acacggaagt gatagaggca ggaaagcctg
      661 agttcccgga tgttataatc acgaatagga ataatctata tgaagaaagt gcatctcaag
      721 tagtggtttg gctcgatccg ccaagagcag tagactgggg gaatcttaca aagattttgt
      781 tgactatcca agaagagaaa tacaaagaca gcaaaattga caagcaaata gacgattttc
      841 ctgaagaaat catcgacagg atcgaatagt gaactttttt tagaaagtcc gactgctttt
      901 tctaaaaata aaaaaacttg gtacgcaatc gctgtaccaa gtttttttat ttagcttttg
      961 attaagccat aaataataat ttatataaga atactgctac gacaccagca cagataggtg
     1021 ctacaactgg tacccatgca tatccccatt gagaagaacc tttatgtttc aatggaagaa
     1081 gtgcgtgtac aagacgtgga gcaaagtcgc gggctgggtt tagtccagga cctgtaggac
     1141 caccaagtga agcaactaat gtccatacaa ggaaaccgag tgctaagtga gctgtaccaa
     1201 gattatcttg gaagaatggt gcttttgtaa tacctagggc accaaagaat aaaacgaatg
     1261 aaccaaagaa ttcattgacg aaaccattga atttagaacc aacgctgttg attgttgaga
     1321 atgtacctaa cacatggttc acgtcttctg tttggtcgta gtaaggtttg taacaagcta
     1381 ctacgatcaa ttgtccaatc gctgcaccaa tcaattgtgc aatgatataa ggaacaactt
     1441 cagcccaagg gaatagacca ttagatgcta aagcaattgt aaatgctggg ttgatgtggt
     1501 taccgctgat tccaccaaaa atcattgctg gcatcataac cccgaatcca tacccgacag
     1561 caatcagcat ccagtcactg ccatatcctt ttgtaccttt taaatctacg tttgctacag
     1621 caccgttacc tagaacgatt agtaatgctg taccaatcat ttccgccgca agacgggtca
     1681 ttagtgagta atccataaat acctccaaaa gttataaaaa tgcaaaaaaa aagaaacatc
     1741 aacacgaaaa gcatcggtgc agtgtttcgt gtcggaagga tttctgcagt cttaacaaat
     1801 gtgaataaaa aaatgaattc cattacttat aagaaaccag accaacagta aaactccaac
     1861 tctttttaaa agttctccac aaatcaccaa gcttaactat aaatatacca aaaaaaaaac
     1921 gttttttttc tcaaaaattg tatcttagaa acagcatgtt ttgaaacttt cagaaaaggt
     1981 ttgttagtaa aatgaaagct atacgtaagg agtgttgttt gatgaaattt tttcaagagt
     2041 ttttcattat tattttcttt tctgtattag gagaaggcat acggattttt gctcatttac
     2101 ctattcctgg aagtatctta ggtattcttt ttttattttt agcatttgaa atgcatcttg
     2161 ttgatcctga aaaaattgga gcaactggtg attttttatt gaataatcta gctattttat
     2221 ttgttcctgc tggtgtaggt ttattggaat attttgatga tattgcttcg atatggcctg
     2281 ttttactggg agcagtcgtc gtgtgttcca tcgtcacgat ggttgcagcg ggaaaaacgg
     2341 cagaaggtgt agaggctttg ctgggctatg tccgtaagaa aaaagcagga agggtatctg
     2401 taaacgaaat ggaggaaaat caaattgact gagttttttg tgaatccact atttggctta
     2461 agtttgactt taggagtgta tgtattatct gataaatttt tgcggaaata taaactgcct
     2521 gttttgaatc ctctcgtatt atcgattgtt gttattatct tatttttgca tttttctggg
     2581 atcagctata aagactataa tatcgggggc aattttttga ctatgctgat tactcctgcg
     2641 acagtggcac ttgccatccc tctttataaa aacttccgtt tactgaaaga gaatttttat
     2701 cctgtaatgg ctgcgatttt agcaggaata gtcgcaaatt gcttggttag tgtagggttc
     2761 ggttacttct tctctcttca taaagagatg gtgatctcat tactacctaa atctgtaacg
     2821 acggctattt ctgtcgattt atctcatacg atgggcgggg tgaatgccgt gacacttgct
     2881 attgttgtct ctacaggtat ttttggttcg ctgatcgcca ctcatatttt ccgcctattc
     2941 aagatcgaaa gtccagttgc tcgcggtgtg gcgttaggaa gtacttctca tgctatcggt
     3001 acagcaaaag caatcgagat tggcgaaata gaaggaatca tttccggact tgcgatatgc
     3061 gtaaatggga tattgaccgt tttgttgctg ccgttattct ttcagccttt tgctggcttg
     3121 ttttgatgcg aaaaccacta ttttatgcta caataaccaa aacgttcgag cataaaaaag
     3181 gagggaaaga cgtgtatttg tcttccgagt atcttaaaca atttgataaa gatctatatt
     3241 ataagattct cctcctagaa agttttgagg atcaagcttg gcataccgct gcacaattag
     3301 cgcaagttgt tcagctggat gcccgcagcg tgtcgaaata cctgaacgag ctgtctaaaa
     3361 actatcagca gtttagcggt aagacacacc ctttgtttac aaaaaatcac cgttctggtt
     3421 ataactttta tgatacgctc gattcaattg aacatgagcg ttttttgatc tatctcgtgc
     3481 aaagtacgct gaaattccag ttgctgcacg acattttttt tgaagaattc catacaatgt
     3541 accaatttgc gcaaaagcat tacattagtg aatctactgc tcatcgaaaa atcaatgaat
     3601 ggaaacagca actgcaaact tacggaattc ggctgcaacg aggtacatat atcgctcaag
     3661 gcgaagaaga aatcattcga ctttacttgc atatgacatt ctggcagttg tttcgaggga
     3721 aaatctggcc atttgaaacg atttctcaaa tggacgtaaa gaatatggca gaacatatca
     3781 tggctttttt taatgttcgt ctgaacgaga tcaaaaaaag acgattggaa tacatgctag
     3841 gtgccttttt tttaagaaaa tcacagaagc attatgttgt attgaatgag aaaaaaagac
     3901 ggctgatttc tgacaatctg ttgttccagc gattttgcca agtcatggag ccggtatttc
     3961 ctaattattt tcaagtagaa gacgagttag gcgcattatt tcttgtactg atgactcgtg
     4021 aagaatatta tagtgatcct aagatacgga agaaaatttt tgattttcat caacaagcaa
     4081 aaacaccgcc ctttacagcc ctttcagaag caaaagccgc tctttctctt tatcaggaag
     4141 agcaggggct gccagcagaa aatctaacct tcgaggcaga aaactattta ttcagcagtc
     4201 attttttcgc ctatcttttt cctaatgcga aagaaacgat tgatggcaat agtagtgatt
     4261 ttattaatca tttagttatt gaaaacaagg aattgaaaca atggctcgta catttttttg
     4321 aaagtagaca caaacatccc aatcatctgg catttaaaaa tcatgcgttt ttgatgggtc
     4381 ggtatctgac tgtgtttaaa acattaggag cctttactcc acagcttcca aagatcacga
     4441 tccttttaat gacagatttc ccattatttg aagagcaact gttagaggag ggattacgga
     4501 catttttccg taatgagtat cagctgattt ttctgccaac agattatcgg gggagagaag
     4561 ttgatcttct gatttcgaca agtaaagtcc accgcaaacc atgggcggat cttgactatt
     4621 ttattgtgac cgaagaacta aaattaattg attacatcca actatctcaa aaatttgaaa
     4681 tgattcaaaa aaaaactatt aaaacaataa caaagaagga gaaaaaagat gacatttgaa
     4741 gaaattttac cagaattgaa aaaaggtgca aagattattc gtaaaggatg gagtggattt
     4801 gaactttatg ttgttctcgt atccgatgat gaatttgatg gctgtccagt gacaccttac
     4861 ttattgatca aaacaagtga cgaaggtttt tccagcttcg ctcctacagt ttgtgatatt
     4921 ttagcagatg attgggcgat cgtagaatga aaaaccagta tccagatcta gctaaacagg
     4981 ttgtatttat cacaggagct gcttctggaa ttggtgctgc gcaagcccga gcgtttcttg
     5041 aacaagatgc ttttgtattt ggattagatc aaaagttcgg taaaatggaa gaactctctt
     5101 tgcgatttcc tgaaactttt gcctacgcac taggtgatgt tcgacaaatg aaagaactcc
     5161 aacaagcggt atcgaaatgc caggatcgtt ttggtgaagt tacgatttta ctgaatacat
     5221 cggggatatt ggatgcctac aaaccattga tggaaactga cgaatcgctg tgggatctta
     5281 tttatgagac gaatgtgaaa agcatgtatc agctgacaaa aatcatcctt cctgatatga
     5341 tccgccgaaa aagcggaaca atcatcaaca tggcttcgat tgcgggtttg gttgctggcg
     5401 gaggtgggat cgcttataca agtgcgaaac atgcaatcgt cggatttacc aaacaactcg
     5461 ctctggatta cgcaggacag ggtatccgag taaaaggaat tgctccagga gccatccaaa
     5521 caccaatgaa tgcggctgat tttgaaggga aaggtgagat ggctgagtgg gtcgcaaatg
     5581 aaacaccagt caagcgatgg gcacagccgg aagaagtagc tgagctgacg ttatttcttg
     5641 cctctcctca agcaagctac atacaaggaa cgatcatccc aatcgatggc ggatggttgt
     5701 taaaataacc ttgtgttttt ttcaagcttc tgtatgataa aactaaagag agaaacaacg
     5761 gttgatgata aaagaaagtc gatgactgtt cccgacattc gatgccgaac agccagttca
     5821 aaaaattctt tttaataatc gttaatattc gaaactaagc ggcttttcac gaccttttga
     5881 tataaacggt gttcaaaagc ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata
     5941 aggagctatt tttatttgac tattcttatt acttgttgca gggcagcttt ttcactacct
     6001 tctgcggcac ttcagttgga attttcttcc catcctttgg tcgcagtcat taccaagata
     6061 aaattatctt gcaaaggaga aatagaacat ggaaaaaaat aatacccgtt cagccaaacg
     6121 ctggacaacg acagatgtga ctcggatggc gctagtcact ggtttatatg tggcagtgac
     6181 gctggttttg tctgtcttta gctttggagt gattcagatt cgtctctcgg agatgttcaa
     6241 ctatctgtcc ttgtataaca aaagatacat cgtcgcggta acagcgggag tagctttggc
     6301 taatatggct tctcctcttg gactgataga tgttgtagta ggaagcgtca gcacgctgct
     6361 tgtattgctt atcaactata aaatcactag tcgcatcaaa aacatgaaaa tcaaaatggc
     6421 tgtgacagca tgcgtatttg ctttttccat gtttacagta gctggtcagc taacgatttt
     6481 atatcaacta ccttttttct tgaattggtg ggtgattgct ttaggagaat tggcatcaat
     6541 gattgtcggt gggataatta tttattggat cggtaaaaaa gtggatttgt caaaataaag
     6601 atctgtttaa tttaaaatat ggcagcgtgg aagagacttt gagtcttctt ccatgctgct
     6661 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     6721 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     6781 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     6841 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6901 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6961 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     7021 caagtaaaag ataaactgca acagatatca atcctagttt tcttccgaga aaagttgctg
     7081 ttaatccaat agcaaatgtt tgccccgtca aaggaaccaa gccaaaagga atggtcatct
     7141 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tattaattct ttcaatgttc
     7201 ggttcataat gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     7261 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     7321 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     7381 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     7441 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     7501 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     7561 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     7621 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     7681 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     7741 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     7801 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tgttaggtcc atgttgacct
     7861 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7921 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7981 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     8041 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     8101 gagttattat ttttctgctg cattatttta cacaaactac agtatatgat agatcatcca
     8161 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     8221 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     8281 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     8341 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttattt ttgactttag
     8401 gaacggctgt ggcatttgca atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     8461 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgctg
     8521 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     8581 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     8641 tatgtggatt tcttatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     8701 tgttagaatc aatagcagta aaactagcaa aacctgcagc aacgattttg gtggaaggga
     8761 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     8821 ccgggaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     8881 acgtgatagc gaattttcct gcctttacgc ttgcttactt tgcttctcaa ggacagacgg
     8941 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     9001 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     9061 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     9121 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     9181 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     9241 tatctatttc tgaagtttta gcttttgttt attattatta tgaacaaatc gaaagagagt
     9301 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     9361 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     9421 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     9481 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     9541 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     9601 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattacagt aagaccgatg
     9661 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     9721 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     9781 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacgag agaagcattg
     9841 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9901 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9961 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
    10021 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
    10081 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
    10141 gtcttgcctg aattagatca gttttttgca gagaaacaag aaatatacca agcgttagca
    10201 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
    10261 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
    10321 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
    10381 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
    10441 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
    10501 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
    10561 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
    10621 gcagatgcaa gcttttgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
    10681 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
    10741 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
    10801 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
    10861 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10921 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10981 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    11041 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    11101 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    11161 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    11221 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    11281 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    11341 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    11401 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    11461 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    11521 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    11581 agtttctgac cgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    11641 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    11701 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    11761 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    11821 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    11881 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11941 gcagaacaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    12001 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    12061 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    12121 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    12181 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    12241 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    12301 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    12361 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    12421 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    12481 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    12541 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    12601 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    12661 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    12721 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    12781 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    12841 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12901 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12961 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    13021 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    13081 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    13141 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    13201 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    13261 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    13321 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    13381 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    13441 agagcgtcaa gaaacaggaa tcaaaaactt aaaaatcgga tttaaccgcg ttttcggtta
    13501 ttatatagaa atcacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    13561 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    13621 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    13681 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    13741 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    13801 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    13861 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13921 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13981 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    14041 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    14101 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    14161 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    14221 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    14281 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    14341 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    14401 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    14461 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    14521 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    14581 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    14641 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    14701 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    14761 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    14821 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    14881 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14941 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    15001 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    15061 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    15121 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    15181 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    15241 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    15301 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    15361 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    15421 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    15481 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    15541 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    15601 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    15661 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    15721 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    15781 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    15841 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15901 gagacaggta cctttttcgt agaagaacca aagaacacat ttgttgacag aattgttgat
    15961 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    16021 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    16081 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    16141 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    16201 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    16261 aaattagaaa cggaaaaacc agtacaacgt tttcttcagt tggaatattt tggacagatg
    16321 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    16381 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    16441 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    16501 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    16561 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    16621 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    16681 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    16741 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    16801 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    16861 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16921 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16981 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    17041 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    17101 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    17161 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    17221 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    17281 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    17341 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    17401 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    17461 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    17521 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    17581 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    17641 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    17701 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    17761 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    17821 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    17881 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17941 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    18001 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    18061 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    18121 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    18181 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    18241 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    18301 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    18361 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    18421 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    18481 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    18541 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    18601 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    18661 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    18721 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    18781 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    18841 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18901 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18961 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    19021 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    19081 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    19141 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    19201 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    19261 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    19321 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    19381 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    19441 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    19501 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    19561 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    19621 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    19681 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    19741 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    19801 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    19861 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19921 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19981 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    20041 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    20101 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    20161 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    20221 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    20281 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    20341 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    20401 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    20461 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    20521 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    20581 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    20641 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    20701 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    20761 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    20821 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    20881 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20941 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    21001 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    21061 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    21121 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    21181 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    21241 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    21301 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    21361 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    21421 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    21481 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    21541 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    21601 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    21661 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    21721 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    21781 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    21841 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21901 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21961 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    22021 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    22081 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    22141 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    22201 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    22261 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    22321 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    22381 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    22441 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    22501 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    22561 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    22621 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    22681 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    22741 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    22801 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    22861 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22921 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22981 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    23041 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    23101 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    23161 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    23221 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    23281 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    23341 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    23401 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    23461 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    23521 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    23581 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    23641 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    23701 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    23761 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    23821 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    23881 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23941 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    24001 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    24061 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    24121 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    24181 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    24241 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    24301 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    24361 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    24421 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    24481 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    24541 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    24601 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    24661 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    24721 tgttaagtt
//
LOCUS       DACDCJ010000042        24294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDCJ010000042 DACDCJ010000000
VERSION     DACDCJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.042"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IV038_001623"
     CDS             <1..572
                     /locus_tag="IV038_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP8341830.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IV038_001624"
     CDS             576..1217
                     /locus_tag="IV038_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341831.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDFIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IV038_001625"
     CDS             1214..2131
                     /locus_tag="IV038_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8341832.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IV038_001626"
     CDS             2128..2799
                     /locus_tag="IV038_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341833.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IV038_001627"
     CDS             complement(3340..3828)
                     /locus_tag="IV038_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP8341834.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IV038_001628"
     CDS             complement(4029..4295)
                     /locus_tag="IV038_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8341835.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IV038_001629"
     CDS             complement(4295..4558)
                     /locus_tag="IV038_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8341836.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="IV038_001630"
     CDS             5034..5219
                     /locus_tag="IV038_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8341837.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IV038_001631"
     CDS             complement(5361..7184)
                     /locus_tag="IV038_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP8341838.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IV038_001632"
     CDS             complement(7782..10487)
                     /locus_tag="IV038_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP8341839.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IV038_001633"
     CDS             complement(10793..11740)
                     /locus_tag="IV038_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP8341840.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IV038_001634"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IV038_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8341841.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IV038_001635"
     CDS             complement(12375..12737)
                     /locus_tag="IV038_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8341842.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IV038_001636"
     CDS             complement(12748..13869)
                     /locus_tag="IV038_001636"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP8341843.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IV038_001637"
     CDS             complement(13885..14235)
                     /locus_tag="IV038_001637"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP8341844.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IV038_001638"
     CDS             complement(14365..15876)
                     /locus_tag="IV038_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP8341845.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IV038_001639"
     CDS             complement(16198..17550)
                     /locus_tag="IV038_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP8341846.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IV038_001640"
     CDS             complement(17703..18779)
                     /locus_tag="IV038_001640"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP8341847.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IV038_001641"
     CDS             18975..20720
                     /locus_tag="IV038_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP8341848.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IV038_001642"
     CDS             complement(21000..21437)
                     /locus_tag="IV038_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP8341849.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IV038_001643"
     CDS             complement(21421..22794)
                     /locus_tag="IV038_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341850.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IV038_001644"
     CDS             complement(23116..23604)
                     /locus_tag="IV038_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8341851.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IV038_001645"
     CDS             complement(23626..24228)
                     /locus_tag="IV038_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8341852.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6967 a         5170 c         4490 g         7667 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgattttatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gactctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctt caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttccaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc ttatgatgtg caccccaaaa gtta
//
LOCUS       DACDCJ010000043        23963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDCJ010000043 DACDCJ010000000
VERSION     DACDCJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.043"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            144..542
                     /locus_tag="IV038_001646"
     CDS             144..542
                     /locus_tag="IV038_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP8341853.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IV038_001647"
     CDS             526..1221
                     /locus_tag="IV038_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP8341854.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2702)
                     /locus_tag="IV038_001648"
     CDS             complement(1815..2702)
                     /locus_tag="IV038_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP8341855.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2887..3726
                     /locus_tag="IV038_001649"
     CDS             2887..3726
                     /locus_tag="IV038_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8341856.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3922..4896
                     /gene="sgrA"
                     /locus_tag="IV038_001650"
     CDS             3922..4896
                     /gene="sgrA"
                     /locus_tag="IV038_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP8341857.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5194..5550
                     /locus_tag="IV038_001651"
     CDS             5194..5550
                     /locus_tag="IV038_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341858.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5553..6710
                     /locus_tag="IV038_001652"
     CDS             5553..6710
                     /locus_tag="IV038_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341859.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6736..7176)
                     /locus_tag="IV038_001653"
     CDS             complement(6736..7176)
                     /locus_tag="IV038_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8341860.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7336..7842
                     /locus_tag="IV038_001654"
     CDS             7336..7842
                     /locus_tag="IV038_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8341861.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8010..9923
                     /locus_tag="IV038_001655"
     CDS             8010..9923
                     /locus_tag="IV038_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8341862.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9928..10929
                     /locus_tag="IV038_001656"
     CDS             9928..10929
                     /locus_tag="IV038_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8341863.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11131..11337)
                     /locus_tag="IV038_001657"
     CDS             complement(11131..11337)
                     /locus_tag="IV038_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8341864.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11983..12831)
                     /locus_tag="IV038_001658"
     CDS             complement(11983..12831)
                     /locus_tag="IV038_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8341865.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12995..13897
                     /gene="gnd"
                     /locus_tag="IV038_001659"
     CDS             12995..13897
                     /gene="gnd"
                     /locus_tag="IV038_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP8341866.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13899..14900
                     /locus_tag="IV038_001660"
     CDS             13899..14900
                     /locus_tag="IV038_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8341867.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14897..15181
                     /locus_tag="IV038_001661"
     CDS             14897..15181
                     /locus_tag="IV038_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8341868.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15214..16011
                     /locus_tag="IV038_001662"
     CDS             15214..16011
                     /locus_tag="IV038_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8341869.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16025..16858
                     /locus_tag="IV038_001663"
     CDS             16025..16858
                     /locus_tag="IV038_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8341870.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16876..17733
                     /locus_tag="IV038_001664"
     CDS             16876..17733
                     /locus_tag="IV038_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8341871.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17755..18264
                     /locus_tag="IV038_001665"
     CDS             17755..18264
                     /locus_tag="IV038_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8341872.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18301..18702
                     /locus_tag="IV038_001666"
     CDS             18301..18702
                     /locus_tag="IV038_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341873.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18814..19167
                     /locus_tag="IV038_001667"
     CDS             18814..19167
                     /locus_tag="IV038_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341874.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19201..19644
                     /locus_tag="IV038_001668"
     CDS             19201..19644
                     /locus_tag="IV038_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8341875.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19661..20746
                     /gene="uxuA"
                     /locus_tag="IV038_001669"
     CDS             19661..20746
                     /gene="uxuA"
                     /locus_tag="IV038_001669"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8341876.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20739..21452
                     /locus_tag="IV038_001670"
     CDS             20739..21452
                     /locus_tag="IV038_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP8341877.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22204..22404
                     /locus_tag="IV038_001671"
     CDS             22204..22404
                     /locus_tag="IV038_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8341878.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(22508..23809)
                     /locus_tag="IV038_001672"
     CDS             complement(22508..23809)
                     /locus_tag="IV038_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8341879.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         8183 a         3725 c         4947 g         7108 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
     2161 tttccataag cttgaacaag cttggagaaa ttttctcctt catttgcttc tttttttgct
     2221 ttatctgctc cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
     2281 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
     2341 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
     2401 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
     2461 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
     2521 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
     2581 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
     2641 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     2701 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     2761 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     2821 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     2881 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     2941 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     3001 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     3061 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     3121 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     3181 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     3241 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     3301 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     3361 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     3421 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     3481 attggtgctc atattacttg gaatcggaca caaggagcta ttttgggtat accagtcagc
     3541 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     3601 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     3661 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     3721 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     3781 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     3841 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     3901 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     3961 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     4021 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     4081 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     4141 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     4201 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     4261 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     4321 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     4381 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     4441 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     4501 agtagcacag gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc
     4561 acaggaagca tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga
     4621 aaagtaaata atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat
     4681 gcagggagca acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat
     4741 catcaagaaa aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt
     4801 agaaccttga acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca
     4861 agctattttc gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa
     4921 ttgaataaaa cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc
     4981 gaagttgtag ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata
     5041 acaggaacac aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta
     5101 gtatctctga aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata
     5161 agtctaaata atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat
     5221 gaaaggcaag tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg
     5281 gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa
     5341 caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac
     5401 aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt
     5461 ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc
     5521 ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag
     5581 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
     5641 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
     5701 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
     5761 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
     5821 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
     5881 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
     5941 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
     6001 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
     6061 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
     6121 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
     6181 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
     6241 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
     6301 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
     6361 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
     6421 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
     6481 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
     6541 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     6601 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     6661 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga
     6721 ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg
     6781 accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct
     6841 gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt
     6901 tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc
     6961 tattctgtga acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc
     7021 gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa
     7081 gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga
     7141 cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt
     7201 tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa
     7261 aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag
     7321 aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa
     7381 ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat
     7441 aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat
     7501 gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag
     7561 cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt
     7621 ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt
     7681 atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga
     7741 gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag
     7801 aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa
     7861 aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat
     7921 cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac
     7981 atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa
     8041 agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg
     8101 gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg
     8161 ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg
     8221 agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag
     8281 aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca
     8341 aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat
     8401 atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca
     8461 agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata
     8521 aagagattat caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa
     8581 acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca
     8641 caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca
     8701 aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc
     8761 atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt
     8821 ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg
     8881 attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag
     8941 ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga
     9001 tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg
     9061 gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac
     9121 taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat
     9181 ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg
     9241 ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag
     9301 atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag
     9361 atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct
     9421 acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt
     9481 atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag
     9541 cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg
     9601 ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca
     9661 aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg
     9721 gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag
     9781 tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga
     9841 aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga
     9901 tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta
     9961 ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag
    10021 ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc
    10081 cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat
    10141 ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa
    10201 ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt
    10261 cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat
    10321 gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa
    10381 gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat
    10441 tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa
    10501 aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg
    10561 tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt
    10621 tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc
    10681 aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat
    10741 ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg
    10801 ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat
    10861 cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc
    10921 aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca
    10981 aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag
    11041 gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg
    11101 tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa
    11161 agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa
    11221 gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat
    11281 agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag
    11341 cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag
    11401 tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat
    11461 aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca
    11521 ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc
    11581 caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa
    11641 taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt
    11701 aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag
    11761 aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg
    11821 aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta
    11881 ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg
    11941 ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga
    12001 aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa
    12061 aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga
    12121 tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga
    12181 agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc
    12241 aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc
    12301 cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat
    12361 aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc
    12421 gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg
    12481 ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc
    12541 catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc
    12601 attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt
    12661 tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg
    12721 actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg
    12781 gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc
    12841 tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag
    12901 agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt
    12961 gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg
    13021 aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact
    13081 cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga
    13141 gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc
    13201 tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat
    13261 tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca
    13321 gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca
    13381 tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt
    13441 caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt
    13501 cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt
    13561 ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa
    13621 tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca
    13681 tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aacggacggt
    13741 tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag
    13801 gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg
    13861 gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa
    13921 tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag
    13981 ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt
    14041 tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt
    14101 ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg
    14161 atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac
    14221 gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac
    14281 aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga
    14341 cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg
    14401 aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt
    14461 tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag
    14521 atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag
    14581 aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg
    14641 atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg
    14701 ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga
    14761 ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg
    14821 tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga
    14881 gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc
    14941 tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata
    15001 cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa
    15061 attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg
    15121 tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta
    15181 gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga
    15241 atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct
    15301 gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct
    15361 gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc
    15421 gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt
    15481 ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag
    15541 attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag
    15601 ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat
    15661 aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg
    15721 gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca
    15781 ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg
    15841 ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg
    15901 gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa
    15961 tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg
    16021 acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt
    16081 tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac
    16141 tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc
    16201 tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc
    16261 tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc
    16321 gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt
    16381 catcaatact tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc
    16441 tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct
    16501 tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga
    16561 gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat
    16621 cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta
    16681 ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg
    16741 gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac
    16801 aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt
    16861 taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc
    16921 acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac
    16981 tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa
    17041 ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac
    17101 actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa
    17161 agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac
    17221 acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc
    17281 gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc
    17341 gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca
    17401 agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag
    17461 acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac
    17521 tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag
    17581 gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac
    17641 ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata
    17701 ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt
    17761 gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta
    17821 tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa
    17881 tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt
    17941 acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt
    18001 ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa
    18061 gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa
    18121 tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc
    18181 gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta
    18241 ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa
    18301 atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag
    18361 cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt
    18421 atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt
    18481 tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt
    18541 catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac
    18601 tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat
    18661 aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta
    18721 gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa
    18781 acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat
    18841 gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac
    18901 agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca
    18961 gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa
    19021 gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg
    19081 cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc
    19141 aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta
    19201 atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg
    19261 gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac
    19321 atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc
    19381 atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc
    19441 aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca
    19501 atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa
    19561 caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa
    19621 aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg
    19681 gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa
    19741 tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat
    19801 tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt
    19861 agcgatccac gatgcaatca aggcagggac agatgaacga gattactata ttgataacta
    19921 tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa
    19981 accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc
    20041 tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat
    20101 acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt
    20161 cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac
    20221 ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca
    20281 tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga
    20341 cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg
    20401 atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg
    20461 catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac
    20521 ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca
    20581 agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc
    20641 tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata
    20701 tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca
    20761 tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa
    20821 aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc
    20881 aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata
    20941 tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag
    21001 tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata
    21061 caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc
    21121 tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac
    21181 cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat
    21241 ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca
    21301 tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg
    21361 taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac
    21421 gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt
    21481 atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag
    21541 tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct
    21601 accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt
    21661 atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat
    21721 tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat
    21781 attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa
    21841 aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt
    21901 ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac
    21961 aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca
    22021 atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa
    22081 tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt
    22141 gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta
    22201 tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact
    22261 ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa
    22321 actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa
    22381 gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa
    22441 gggctctttg tcaaatagga ctgatagagt gagataagaa catctaaggt agaattcctt
    22501 ttctacttta gatgtttttt gattcatagg taatcagatt atttagtaag aaaattctgg
    22561 tcttcatttt ccgaaagctt cgaaagccat aagcgactcg tttcaatgct ttgatgtggg
    22621 tatgcagatt ttctattttg gcatttgaat aaggcaattc gagggctaaa cgaatgccgg
    22681 attcatagtt tataaaagct ttttttatag aatgcagttc ttttggaacg gaattgggca
    22741 tcgttcttac aagtttaaag aaagtgtccg cctctttgtc gtgaaacgct tgaagaattt
    22801 cttgatagta aaaataggcg attcgtaatt cgtcagagat cgaaagcaga cgatccacca
    22861 cttcactttc ggtgagatac gcccaaccaa aattgcgcca tttggtccat ttgtcataaa
    22921 caagcttgtc ctgtcgtttg atcagaagcc gagacaacgc ctttaattgt cgataatatt
    22981 tggcttgtgt tggatcgttt cgatcaaagc ttttcatgat tcgtacgcga atatcctcaa
    23041 atttacgaat gagatgtttg acaatgtgaa aacgatccac aacgagtttt gcctttggaa
    23101 aacattcctt tatcagagag gcataagaaa cattcatgtc cgtcacgatg acctttacct
    23161 tcaatcgtgc gcgtcgttca aattgcataa aataagaaac gagagccttt ttcttccgtg
    23221 aaggaagaat atccagtaat tttccagtat cgccatccat acaagaaaag gagtagggac
    23281 cgacttgatt tttcagtgaa cgaaattcat ccattaataa acaacgcggt aaccaatttc
    23341 tttttacttt tgtcatgggg atcagagaag cgagtgtccg agaaacggtc gaccaagaaa
    23401 cgccgcaatg cttagcaatt gtcgtcattg cttgtttttc agttagctct tctaaaattt
    23461 tatagcgcac ttgtttagcg ataaagcagt ttctatcgac aatgtaggta ttcgccgtaa
    23521 aataggtgtg gcagccacga cagtggtagc gttgtttcgc taaagcaaga taagtcagtt
    23581 ggttcgcaca tttattaagt tgaatgagcg attttttctt tccgttctta acgatcatgc
    23641 cttcatgatt catctgttta cacaaaggac agcgacgaat ttctttactt aacgtcgcag
    23701 aaacaacaag actcttgata ttcttaatgc tcttcgattc cacatttgtg atgaaaatgt
    23761 ttttatcttt gattccaagt agtagtttga tatgatgaag atcaggcata cagaacgctc
    23821 ctttgattgt gttgtggtga ttcaattcta acggagcttc tgtatgtttt gttttttcat
    23881 gctcaaaagg gaccgataga atgattttct atcagtccta gaaattatag agccaagaaa
    23941 agacggtttt tttgtacgaa aaa
//
LOCUS       DACDCJ010000044        23962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDCJ010000044 DACDCJ010000000
VERSION     DACDCJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.044"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(230..1522)
                     /locus_tag="IV038_001673"
     CDS             complement(230..1522)
                     /locus_tag="IV038_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8341880.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1519..2142)
                     /locus_tag="IV038_001674"
     CDS             complement(1519..2142)
                     /locus_tag="IV038_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341881.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2145..2981)
                     /locus_tag="IV038_001675"
     CDS             complement(2145..2981)
                     /locus_tag="IV038_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8341882.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2998..3885)
                     /locus_tag="IV038_001676"
     CDS             complement(2998..3885)
                     /locus_tag="IV038_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8341883.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3901..5184)
                     /locus_tag="IV038_001677"
     CDS             complement(3901..5184)
                     /locus_tag="IV038_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8341884.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5867..6550)
                     /locus_tag="IV038_001678"
     CDS             complement(5867..6550)
                     /locus_tag="IV038_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8341885.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6568..7185)
                     /locus_tag="IV038_001679"
     CDS             complement(6568..7185)
                     /locus_tag="IV038_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP8341886.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7195..8019)
                     /locus_tag="IV038_001680"
     CDS             complement(7195..8019)
                     /locus_tag="IV038_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP8341887.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(8012..8572)
                     /locus_tag="IV038_001681"
     CDS             complement(8012..8572)
                     /locus_tag="IV038_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341888.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8746..9702
                     /locus_tag="IV038_001682"
     CDS             8746..9702
                     /locus_tag="IV038_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8341889.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(10007..10963)
                     /locus_tag="IV038_001683"
     CDS             complement(10007..10963)
                     /locus_tag="IV038_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP8341890.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            11067..11669
                     /locus_tag="IV038_001684"
     CDS             11067..11669
                     /locus_tag="IV038_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8341891.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11757..13256)
                     /locus_tag="IV038_001685"
     CDS             complement(11757..13256)
                     /locus_tag="IV038_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP8341892.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13272..13796)
                     /locus_tag="IV038_001686"
     CDS             complement(13272..13796)
                     /locus_tag="IV038_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341893.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            14094..14843
                     /locus_tag="IV038_001687"
     CDS             14094..14843
                     /locus_tag="IV038_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP8341894.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14834..15619
                     /locus_tag="IV038_001688"
     CDS             14834..15619
                     /locus_tag="IV038_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8341895.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15588..16304
                     /locus_tag="IV038_001689"
     CDS             15588..16304
                     /locus_tag="IV038_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8341896.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16406..17425)
                     /locus_tag="IV038_001690"
     CDS             complement(16406..17425)
                     /locus_tag="IV038_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP8341897.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17444..18502)
                     /locus_tag="IV038_001691"
     CDS             complement(17444..18502)
                     /locus_tag="IV038_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP8341898.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(18723..19871)
                     /gene="nagA"
                     /locus_tag="IV038_001692"
     CDS             complement(18723..19871)
                     /gene="nagA"
                     /locus_tag="IV038_001692"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP8341899.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(19947..20783)
                     /locus_tag="IV038_001693"
     CDS             complement(19947..20783)
                     /locus_tag="IV038_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8341900.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(20967..22184)
                     /locus_tag="IV038_001694"
     CDS             complement(20967..22184)
                     /locus_tag="IV038_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8341901.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            22567..22650
                     /locus_tag="IV038_001695"
     tRNA            22567..22650
                     /locus_tag="IV038_001695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22601..22603,aa:Leu,seq:gag)
     gene            complement(22890..23888)
                     /locus_tag="IV038_001696"
     CDS             complement(22890..23888)
                     /locus_tag="IV038_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341902.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         7243 a         5001 c         4103 g         7615 t
ORIGIN      
        1 ttattttttt gtaaaatcta atgattttta atgcattatt cctttttgtt cgggtttaaa
       61 tagttatttt tattttctcc tatctaaaaa agcagcccac ataaaaagaa ggcatgacaa
      121 aaagcatgtc atagcccctt tttccgaata aatagtgttc aaaagacagc ctatcggcaa
      181 taaggcaaga ttttataaac tacgggaagt aatttccgta aatctattct tattctttta
      241 agctgacaag ctcgtcaatc actactgctt ttcctgaagc aatagaatga tttgctgcga
      301 ttcctgtcaa aatcgacaaa ataccatcta cgtgagttgc tgcccgattc cacggatctt
      361 cttctttttc ttcaccaaat agatcatcta ataaacgcgg atctcctccg ccatgtccgc
      421 ccgcttcttg ttcgacgatt acctcatatg gctcaccgaa catcggatag acatggatgg
      481 actgaagaac ggctgctcct tcatcctttt tatctccacc tgcattgatg taagactttt
      541 cgatcacttt catttcgatc cgtcctttgc tgccattgaa agaaacattg aatccttccc
      601 acggcatata cgcgtttagc gaatacgtca aaatcgcacc agtttcatat ttgaccgtta
      661 cgcctagtgt atcttcaata gagatatcat cggaaaatac actgcggtca cgaatatatc
      721 cactgtcttt ttctgcttct aaatacattt ctcgcaattc ctcattggct gacaggtcta
      781 tagcaaatgg atcagcttta gctagttcgc ttccatagca tcgactgtaa aatatttcta
      841 ctccgcgttt ctccgcatta tgcatcccat aaaagtttaa tccaccgaat gcaaatacct
      901 tttggggacg gctgttcaac caaaaattca ctagatcaaa atgatgggta gatttatgaa
      961 caagaagccc gccgctattg tgcttatttc gatgccatct tctgaaataa tctgccccgt
     1021 gttgcgtatt taacagccat tcgaagtgga tagaaaacac atcaccaatc acatcacttt
     1081 ctatcagttc ctttatcttc gtgtgatacg gtgcataacg ataattaaaa gtgactcgca
     1141 ggcttcttcc cgtctgtttt tggaccgtta ttatttcttg agctttttcc tcatcgatag
     1201 tcagaggttt ttcgcaaata acatcatatc cttttttcat tgcacgaatg atataatcat
     1261 gatgcgttcg atctactgac gtaataatga cttcagccgg ccgtacgtca tccagtagtt
     1321 gctcaaattg tgatggcata catgtcttta cttttttctg tccatacttg ttcacaagta
     1381 tccgatttgc ataatccatc cttgtttgag atacatcaca aaaaccgact agttcacaag
     1441 tttgatgata cgtagaggca attgcctcat aaaaaaaacg cgctctccca ccaagtccga
     1501 cttgtacata ttttttcttc atttaaagtc attctccttt tcttttaagc aaaatactgt
     1561 gacaaatggc agccctggat acagtaatgc agtgagtaaa aaatcatgct gtatacacca
     1621 gaacagtcca tacaaaaggc ataaatacca cagaaacccc ttgacgtttt ttgacaacag
     1681 ccagatattc cttttgatca aataaacaag actatctgct ttatcttctt tgcagatatc
     1741 aattagcgta ctaaaaaaac tatgggatac aaaaagcaac agacacaata gaaaaggaat
     1801 cagataccaa gctccaatct ttcccactaa taaataccaa tcaactgcaa aaataatgac
     1861 aaacagctga aacaacgtaa tgatgaaaca catccgtttg atttttcctt tcctccaaca
     1921 tacctttttt tctgccaagt aatggacaga tagataataa gaaggataca tcatcaaaaa
     1981 ataaatcaga tagacaacac gagcagacag cactaatcct aaagtgaaca aacatcctag
     2041 aactaaagcg aaaagagcat tactgataag actggttaaa aaaatccgac agacatagta
     2101 gttgagcttg taaagaaaca tctcttctat tggctttttc atattcagcc cttcagaccg
     2161 cttgtactga tcccctggac aagatattta ttgaagaata agaaaatcag aaaaaccggt
     2221 agcagcgaca acgtagacat cgcaaacata ctcgcgtaag tcgtactaga tccagcgtct
     2281 gcaaataatt tgatggcaat cgataccgtg taagatctcg gcttgtttaa ataaatcaat
     2341 ggtcccatga agtcgtccca tttccaataa aactggatga tgatcgtcgt gatgatcgaa
     2401 ggtttcagca aaggaaaaat aattttggta aagacactat acttgctgca tccgtcaata
     2461 atcgctgatt catctaactc tttggggatg ctgaccatga attgcatgat ctggtaaatg
     2521 aaaaatgctt gtccaaaaaa atgtggtaaa atcagcggca catacgtccc aaccaaattc
     2581 agccgattgt aaataataaa ctgcggaatc aaaatcactt gtgcaggaat catcatcgtt
     2641 actaacatga cgacgaacca gaatcttttt cctttgaaat cgattctcga taatgcgtaa
     2701 gcaatacaag tcgagctcag aactgtccct atcgtcgaaa ctgcagagat gacaaacgaa
     2761 ttcctaaaga aagtaaaaaa tgaaattcca gcaaaccccg cccatccttt tgcgtaattt
     2821 tcccattgaa aagtctctgg aatcaaattc agcgaaccac tcataatctc ttgatccgtt
     2881 ttaaaagaac ctgcgatcat ccataagacc ggataaatca tgactagacc taatagcaaa
     2941 ataaaaccgt attgaaacca tttcgttact gtattattca tttcttcctc ctctcattca
     3001 cacaacgacc catttttttg atgatttgaa aatcagaaag gtgacagctc ccataatcaa
     3061 cagcatgatc cacgaaatag cactagcata ccccatagaa ttaaaagaaa aagcttggtt
     3121 atacacataa agagcataaa agttcgtggc atcattcgga ccaccttggg taatgataaa
     3181 tgcctgcgta aaagtcataa acgccgcgat tgtctgcata accaagttat acaaaatgat
     3241 cggcgaaaga agaggtaacg tgatcttgta aaacgtctgc catttgttcg ctccatcgat
     3301 ctctgccgct tcataatacg agcctgggat ctctttgata cctgcagcaa agatgatcat
     3361 tgccgaaccg aattgccaag cagacatcaa aacaagaggg atcatgacca gtttttgatc
     3421 gctaaaccag gaaagcgcag gtgcgcctaa tcgttccagg aaaatattca ctaacccgtt
     3481 tttagaaaac aactcccgcc aaactagagc gatagaaata ctgccaccga tcaaactagg
     3541 cacataatac aaagaacgaa agaaagtcgt tcctttgata ttcctcgtta aaagataagc
     3601 aatcaataga ccaaagatca gtttcacagg tacagatgca aatacataga gaaaagtgac
     3661 tcggatggag ttcaaaaaac gggcatcact cagcatcttt atataattat ccaagccgat
     3721 ccatgagact ggatttccca aacgataatc ggtgaacgaa taataaagtg acaagatcat
     3781 cggtatcact gttatcaata gaaatcctag gatgaatgga gaagcaaata cgtaaccgac
     3841 cacattgtct tgatccaaga accgttttag tttttctttt ttcatgcctt cctcctgctg
     3901 ctatcctaga atgtttgacg caaactcata aagactctct gctgcttctt ctgctgttac
     3961 ttcttcataa atgacttttt caactaaaag attgtattgg tcttgaattt ccggattcaa
     4021 agcactatca aagacattca cttcgccgtt gtccatatcg gaaatcattt ctaaataatc
     4081 aaatgtcgct tgcaaagttg catcatctgc acgagcgagc tgttctcgaa tattcgacat
     4141 gattggtaca ccacgttctc cttttaaaat ttcattggct tcttcactgt tttgaaagaa
     4201 atcaataaac ttagcagctt cttctttatg ggctgagctt tttgccatac tcagcatttg
     4261 cgaagaacgt aaagggatac cagattctcc atctgctttt ctgcgaggtg gattaatcaa
     4321 ctgaatctcc atatcttcag gagcccctga caatatttct gtcaactggt tacttgatag
     4381 aaacaccatt gccgcttctc tatccctaac cgcctgaaaa gacagatccg tcgtacttgc
     4441 agctccacca cctggttctg gatgggcccc tttgtctacc agatttttac gcagctggaa
     4501 aaaatctgtt agatagtctg gattttcaaa tcccaagctg ttagatagat cttcccgatt
     4561 ataaaaattc aatcctttct caacttgtgg tatttgaata atacagccag ctataaaatc
     4621 ttcgaatctt gaaatagcat agattcctaa ttcttcagtg atttgatagc tgatttgttc
     4681 aaattcatcc catgtccaat ttgaagctgg ttcagggata tttaattgtt caaatatatt
     4741 tttattgtaa gcaatcgcaa acgaattcac cccgttagac agtccaacta attgcccgtc
     4801 gtaagtcgtc gttgctaaat aggattcatc tgcattagaa atatcaatta tcccttgctc
     4861 cacatattca tctaatggtt caatctgatt gatgtattga ggaaattgat tgttcagttg
     4921 gaaaatgtcc cagatatcat ctgatgctgc tagtgtacct aattttgtgt aatatccttc
     4981 ataatcataa aattcataat cgatcgtcac atggggattt ttttcttcat aaagctcgat
     5041 tacttttact gttgcatcgt gtcggacttg cgaaccccac caagccatgt tcagcgtgat
     5101 ttttttgtca tcttcttgcc cacaagccgt caagaagaat aacataacac ttaaagctaa
     5161 gatgattctc attcttttct tcattctctg cacctctact tccattgtta tttttcaata
     5221 tactgtattt ttatacagaa catatttatt aaacgatctt tttcaagggg aataaaaacc
     5281 aagacttgcc ttatcgttct gcctaggatg aacaactcaa tactttcaag aaaaattaga
     5341 tagtgctgat tcttctttca tcgctaaaaa cacttcttgc ttcaatgatg ggaggataat
     5401 tacgcaaaga aaaaaggata tcagacaact gcccaatatc cttgacatag ctaaataagc
     5461 atgcttcttc gtgtgcgctc ttccctaaaa aacagaaaaa taactagtaa ttctgtatga
     5521 aattaggtac aatatacacg tcgatgcaca agcattgact atggcaactt gattggtcag
     5581 ttcccgctga cttttcaagc cccgacaatt tagctgctac taaattgtcg ggtgcctttt
     5641 ttagttttta ttcgtttttc attctataga aagcgcttca ataaatcaag acattttttt
     5701 gttagaatgc taataataac aaaaggtttt aactcttctt atgtacatta caggtaaaaa
     5761 aacaaaaaaa tttctctttt ttttgaattc tgaacaaata gtactggagt acaaaagacg
     5821 aggaagagcc actatctgct cttcctcgtc ttttgattct cacctgttat tcatgattct
     5881 ttttctcagt ttcccgatat ttttccattt tacgactaaa aagctctccg ctacttggtg
     5941 gcgtatatgt gatggcattt gctcctgctt gaatggtctc agcaatactt tcttccgttg
     6001 gaccacctgt cgcaatgata ggaagttcag gatatttttt acgaaaatgg cgtactgctt
     6061 ctgcagtttc tttacctgca ctgatattga taatcgaaat accagctgct aacttttcat
     6121 ctatttctgt atatttcgac gttaccgtac tgataactgg aatatcaact actcgacaaa
     6181 cttcttttac tgtctctaaa ggcgtaggac cgttcaaaac gacccctaac gaaccttgcg
     6241 cttctgcgaa catgctcatg taggaagaac gagcaccttg tgtcaaacca ccacctacac
     6301 cggagaaaac tggaatgtca gctgcctcaa taatgctttt agtaatcgct ggatgaggtg
     6361 taaaaggata cacagcaatt actgcattcg catttgtatt acgtatgatc gcaatatccg
     6421 tagtaaagat gatcgaacgg atcaattttc caaatatctt gatcccgctt gcttctaaaa
     6481 tgatttccgg tactcttacg atatcttttc tcaattccgt catgatttct ggcgtaaatt
     6541 gttctgccat cagccacttc tcctttgcta tctccggtca tactgattca attgatacgt
     6601 ctcgatgacc tggatgattt tttgcgcata accggggtct gtcgcataac ctgcatcttg
     6661 caatgcttgt gcggcttcct gatagttggt ggccgtaatc actttttcat acagcgcagg
     6721 attccaatct actccttgga cgaacagttg tgtatggtca tccatggact gttcccatga
     6781 atcatacact ttgaagtctc cttggatagt tatccattct tcgttaacaa attcctttgt
     6841 ttccaggctt actttcttct gatccccata agccttgatc ccgaaaagat tattgtattt
     6901 gctagccaag gtactttttc cccaatttga ttctaatata gcttgtccta aaatgatact
     6961 aggaagcact ccataaccat cttgtagttc ttgggcatgc ggaacaagcc gttcaataaa
     7021 ttgctgatgg gattgcgctt cattttcttg ctgattatca gtaaaaggag aagacagatt
     7081 cttcaaggaa aaaacaaagg ctaagagtat cactgctaca ccggcaacca ctgctggcca
     7141 acggatattc ttgcgctttt gctttttcca cttttttttc gccatgatca attcctactt
     7201 ttcatttaat tcattcagat attcttctag cgtcaattgg tgtttggtga tatactcagc
     7261 gttttcttta ccgacataac gtaagtgcca aggttcatat gtgatcttag ttatatcttc
     7321 gtgtcctttt ggataacgaa cgataaatcc atactgcgca gcattatctg cgatccattt
     7381 agctccaggc tgatctccgt aacttgcatc caatacagta gaaggataac tgttgtacca
     7441 gttttgatcg acgacatcga ctgccagacc tgtgtggtgt tcgctgtatc ctggctccgt
     7501 aatcgtttct ttagtaatct ttgtcgcctc ctcttctgat acaccttgac gactcatcac
     7561 atcttggaca ttttgttcga aaacttgttg ttgggatgat acagaccggt aagctgagac
     7621 catcactaat ggaaatccag ctgcttcagc agccttggca aatgcttcat attctggagc
     7681 aatgcgtttg tcgatcaagt atccgttact taatgatgcc aattgagtag actcatcaat
     7741 ttctttttct aatttgtgat ctggaccaac caaaactagc ttccaatcgt ctgttttaac
     7801 atctggcaat tgtttggacg tacgatcttt tgtttctttg tcctttacct tgctgctggt
     7861 tgtttgactg ttcttttctt tactagcagt tgttttggtg cttgttcgtt cgactgcttg
     7921 attgccggct tgaacatgcg aatcatcatc gctaaaaata acatagccca ttgctgcgat
     7981 cataatcaca actgctccaa atcctaaaaa tttattcact tgaaaaatcc tttcgttgtt
     8041 cttcacgttc cccattcgcc tcatgaacgt aagtttcttc cagattttca ttcacccaat
     8101 cgagaagttc gattttttct ccttcgatct gtttttgaac atctgctggc agacgaaaat
     8161 tcaaaatatc atcatagccc cattcatcat attgaatact tactttcaag tcaaggtatc
     8221 taccagcctg ctgattattt ttcaaaggga tcagctcgac ttttgctgct ttgctgctaa
     8281 gccatttttg cgcgatgcgt gttttgattt gcttgtactc tgcaacggca acgtttcgtt
     8341 tttcgggaaa aataatcgtt tgacgcaaaa tcttttgcgt tagcatccat tcataatcag
     8401 tgaaaagatt ttttactttc tgcatcgctt cagatggcag atttttctcg ccatctttcc
     8461 atttttgcca ttgttctgga ctgatcagca agtattcatc taaaaaattt ttttcagttt
     8521 caaactgttc taataataca ctcataataa gatcaatata tacttgatgc acgtttatcc
     8581 cctcctttta actattctac ttaagttttg atcaaaaagc acctcttaaa tcgctgcttt
     8641 catttttttt aatgtttcaa gtcctctcct ctttattctt cgtgattttt gttataataa
     8701 tagacagaca agaaactcaa tccaactatg aaggagcgtt ttcagatgaa aaaatatcaa
     8761 tggggaatcg ttggtcttgg taccatcgca catgagtttg ccgaaagctt caatcaagag
     8821 acgagcgaac taacagcagt tgcttccaga acttcagaaa aagcggaaaa cttcgctcac
     8881 cgttacaata ttccaaaagc ttacggaagc tatcaagaaa tgttagacga tgctgaaatc
     8941 gacatcgtgt acattgctgt accaaataga cagcatatcg accatatctt ggcagcattg
     9001 gaagcaggca aacacgttct atgtgaaaaa gccatcacga tgaataaaaa agaactggct
     9061 gacgctatgc gcttagcaga agaaaagaat ttgatcttag cagaagcgat gacaatcttc
     9121 aacatgccac tttatcagca gttacgctca ataatggata caggaaaact gggggcttta
     9181 aaaatgatcc aagcaccttt cggcagttac aaagaaccag accctaaaaa ccggttcttc
     9241 aatccagaat tagctggagg agctctgttg gatatcggta cgtatgctgt atctttcgct
     9301 cgtttctttt tgagctctca acctgaagta gttgcctcta caatggttcc ttttgaaaca
     9361 ggcgtggacg aacaatccgt cactatcttg cgtaacaaag aaaatgaact tgctgctgtc
     9421 agcttgactt ttcaggcaaa aatgccaaaa gtcggtatcg ttgcctttga gaacggttat
     9481 gtgacgattg ctgattaccc gcgtgctgat cgtgcggaga tcttatttac agatggtaca
     9541 aaggaattca tcgaaagcgg gaatacatct gaagcaatga attacgaaat aaagaatatg
     9601 gtacacatga ttgaaggtga cttgcccaat cgctctctct tcttgacaaa agatgtcatc
     9661 gaaatcttgg atcaaatgca agttctatgg aaaaatatgt aattttaaaa ggttgtgaaa
     9721 aagctgttct gcatcaagta ataagaacag ccaaacaaaa atagctcctc ctatttttcg
     9781 tttggctgga cttattaccg cagaggaaag cttttgaaca ccgtttaaga aagaggttgt
     9841 gagcctaccg ttcagctccg agtcacaagg agcaatttca aaaaacagct tctcatgttt
     9901 gttgaatatt gtgacttgtg accgaggagt tgacaggaga acaccgttta aagaagaggt
     9961 tgtgaaatca gctttttgac ctgaatatta gctgaattca caacctctat ttttggattt
    10021 tttgttggga gaattctgcc ttgatttcaa taaaactttt aaaagactgc aaaaactgga
    10081 acaattctgc tcgatcctcg aattcagggc gactagtcgg cagaggcatt tgccgataca
    10141 atacataaac ttcttcaata cgtgttaaaa tttgtctgcc atcatttgac tctgaaaatg
    10201 tttctgcagt ataaatcaat aaaccataga tatgttttcc ataataagct ggttgctgga
    10261 ttctctctag attttccagc atgtctttaa tcacattggc ctgtgctctt ctcatagaga
    10321 agtaagtttc gtagtatagc ggctgtctga gccagtagtt ttcttgatgt tctctggcat
    10381 cttcttggct ttcgcgaatg aatgtcagta aatgttcgca tttgatctgc acatcttctt
    10441 tattttctga taaaagaaat tctgccattt gtctcaaaat atttcgaaat tctttttcga
    10501 tctgtatttg gttttccttc agctgcttcg tacgatctgg catgtaaaca tttgcaagca
    10561 aagcaaatac taatccaatc gccattaatc ccgcttcatt gccgatcagc tgccaagaca
    10621 tgttttcctc cactaaataa tgagtgacca aaaccgaatt tactactatc ccttcggtta
    10681 atttgaagcg agaggcaagc gggatgaaca ggaataaaaa aagactgaag ccaaaaatag
    10741 tatacccaac agcaaaaaaa ctgaacagtg ctgttgccgt agctaatata aaggctgcga
    10801 ttcgattcat gcccgtacgc aatgtagagc gcttggtatt tcctacactt aataatgcaa
    10861 tgatccctgc agctggccaa tacaacagcg ataagctacc cgctgtgacc atcgaaaaga
    10921 agacactgat cactgttttg actgttcgca ttcctatacg catttacttc cccctttttt
    10981 attttaatta tacttgaata atgaaaaact agctatcaga caaaagttta ggtatgatag
    11041 taaaaagacc aaaaaggaga atctctatgc aatttggccg ttttcgacta ggtatgcgta
    11101 cgatcaaaag tgccttagct gtttttttat gtatcttgtt ttttcatttg actcaacgag
    11161 gaatcccgat gattgctgca ttatctgctg ttttttcttt gcgtcaggac ttgactacaa
    11221 ccgtttcttt tggaaagtct aggatcatcg gcaatacatt aggtggtaca ttagctatca
    11281 tttatttttt tgtcaaaagc tattttcaca atgatttttt ggtagaattg atcttgcttc
    11341 ctattctagt tattattgtg atcgtcgtgt cagatggtat taacaataat tccggtatca
    11401 tttccgcaat agctacgttg ttgctgattg cgttgagcat ccctcaaggc gaatccgctt
    11461 tgtatgctat ccaacgtgta ctagatactt ttatagggac attcattgca atcggtatca
    11521 atttctttct tcgtcctcct gaaacagaaa agaaggaaga actatcagaa gatttggtcg
    11581 aactccaaaa gaaagaacga ttgctaagaa caaatttaca agagatagaa gctaagatcc
    11641 aaaaacaaaa gaaaaatagc gaggactaat tttcttttgc aataaaataa aaaaatggag
    11701 agaagaactt taaaaagtct tcctccattt ttgattgaac ggtgaagtgt actactttat
    11761 tcttccttca tcgagttttg ttctgcctct tttggcgctt catacatatt tacttggcta
    11821 gtagtcccac gtgtcgacaa attctctacg atttccttca agtcaagtcc tgttgtcgct
    11881 tttagtgttt cttgagtaga agaaagcaaa tttgtggcat aatttgtcac tcggttcgct
    11941 ccactggttt ctcctgaact gcttcctgta tcgacaacag agattttttc gatatttcct
    12001 agcggctgtg ctgcttcgcg catcaattgc ggtagcatat cgataaccat actcaagaca
    12061 gctgcttcgc catattcctt gaaggcattg gcgatctttt gttttgcttc ggcttctgct
    12121 tttccttttg ctagtgctgc ttcggcttct gcttgtccag ctaaacgtgt cttattggct
    12181 tccgcttctg ccagtgcttc cacacggaat cgttctgctt cggcttcagt cacttctttg
    12241 actttttgag cttgtgcttc ttgttcttta gcgtaccgat cggcatctgc ttttttcttc
    12301 acttccgaat catattgctt ttcacgacgt gtgatttctt tttcttccag ttcgatttgt
    12361 ttttgacgtt ctacgatttt aatctgcatt tcctgttcga ttacttgttg ttgtgctcgg
    12421 gcactttcta aattgtaggc ttggtctgct tttgctttag caacatcttg ttcttgtttg
    12481 taggttgcta atttcagttc tttttctttt aatgattctg cgatctctgt ttgtcgctgt
    12541 aattcagctg cttgtgattc tttttctgct tgagcttttt tgatacgcgt ttcttttaga
    12601 gcttccgctt ctgctatatc tgcatctctt ttgacttggg cgattcttgg tttccctaaa
    12661 gagtcaagat aaccgttttt gtctctgacc tctttgatag tgaaagaaac gatgatcaac
    12721 cccatttttg caaggtctac agaagcgact tcttgtacgc tttgactgaa cttatcgcga
    12781 ttttgataga tttcttctac agtcatagaa ccaagaatcg agcgcaagtg tccttctaat
    12841 acttctcttg cttcattttc cagttcttct cttgtcttgc ctaaaaactg ttctgctgct
    12901 gtagcaatct cttctacaga tgaaccgatt ttgatgatcg aagtaccatc acacatcacc
    12961 ggtacgcctt gttccgtata cacttcaggg gtggagacgt ccaatttact cgataataga
    13021 ctgatccgat tcgaacgttg aaaaactggc aagacgaatg cgccacctcc acgcacaatt
    13081 ttgattttgt tattgctttc atcaatgtgc acatttttgc ttcctaaata acttccactg
    13141 atgatcaacg cttcatccgg ttttgctgtc tggtacttcg ctacaaagac gatcagcaac
    13201 atcagtaaaa tcaaagctgc gattgcgatc gcgataaaaa caactggtat ttccatttaa
    13261 cacacgctcc tttaagcttt tatcatgctt tcataaggca tgacataaca gattctttcc
    13321 ttgatctcaa tgatcaatac tttcgttccc cgctcagctg tctgttcttg ggggctgtaa
    13381 aaacaagctg gacgactaat ggaaccggta acacttttca actgaatttc tcccatacct
    13441 cgtacaggga tcggtgtgat cacaacagct acttgtcctt ccagagtttt ttcagaagac
    13501 gacatcgttg attcggcatg tttcatcggc atcaatatat agaaattaag caaaaatacc
    13561 aacactatcg atatgcctcc actagcaata aataaaaata aagatgattg atctgttaaa
    13621 cgctcaccta gaaaaccaaa taagcttgtg aaagttaacc aagggataat aagcatcgga
    13681 tcaagcgggc catcaaaatc gaatacatct ccaaaaataa tcaataggac tgccaatcct
    13741 gcacaaatca gcaatgtata ccaataaatc gtctcaagtg cgattccccc tatcatatac
    13801 acgcctcctt ccatttctac gtctatcata ccaatgtttg aaaattctgt atagcttctt
    13861 ttaccgactc agtggtgttt tttataggaa ggttgtgttt atcgttgaca atttgttaaa
    13921 tttttgtttt catgttaaaa ttttcacaat aaattctttc gaggtagcct atgtttcctt
    13981 tttttaagaa aaaaccatta aagctccaga acacgatcat ccttgtcgtt tgtttctcca
    14041 cattcgtttc tttgattgtt tctgccatat tgatcaggaa ttttattctt tcgatgaacc
    14101 gatctagtgt gtcagataaa atctctagca ttgctaaaat cgttgccaat gataaactca
    14161 tccaaaaaaa gctgcagact ggtgacccga cacatgcgat tcaaggaata acaaatgaaa
    14221 tcatgattgc atcgaattta gattttatcg ttgtcatgga ccctgaaggc attcgatatt
    14281 ctcacgcaga cccaagttac atcggtcttc ctttctcaag tcatgaagat gtgaaaaaag
    14341 tgattggagg acaagaattt ttctctgaaa aagaaggaat gttagggttc ggcttccgct
    14401 atttcgtccc tatttattat gatcaagaga ttattggtgc agtttgtgtt ggcttaacaa
    14461 acaacacgat tgacaatcaa gtcaaagatg gacaaaaaat gattgttttt tcattgatta
    14521 ttggacttag cgtaggcatc ataggcgccg tttttcttgc ccaaaaaatg aaaaaagtac
    14581 tgcttggact tgaaccacaa gaaatagcag aacagttaag tgaaaaaacg attatcgaaa
    14641 atgaagtaga tgaaggaatc ttagccatct cttctgataa aactatcctc ttgatcaata
    14701 cacctgcaaa aaacattttc taccaaacag agcagctatt ggatttcgaa gtaggaaaaa
    14761 aatcagtgat aatctatatg acgttttgtt taaagagacg attgagcatc aaacgaccgt
    14821 tcggactcaa tccatgtatt taaatgggat agaaactatt tctagcgtta cacctctttt
    14881 tagcaatgac cgttttttag gagcagtcgc aactttacga aatcgatcag aagtaatgaa
    14941 attaatggag gagcttgggg ggacgaaaca atatataaac tccatgagag ctcaaaccca
    15001 tgaattcatg aataaacttc atgtcatttc tggtttgatt gatttgaaga agtatgaaga
    15061 agtcacccat tttatccagc agctaaatca aaattataaa aaagagattg gccaagtctc
    15121 aagtctgatc aaagtacctg caattgccgg ttttttatta ggcaaaataa acgaagccaa
    15181 agaacaatct gttgaaatgc tgttagaaag cgattctttt atccctaaaa tggagatgga
    15241 tgactccatc cattcacttt tgcaagtgtt agggaactta cttgataatg caaaagaagc
    15301 cgtgctacaa aaacatgaaa gaaacggaca ggtccggatt catttaaact atgacacaga
    15361 agctgaagta ttcatcgtga ccgtctgcga tgacggtgtc gggatatcag aagaaatctc
    15421 agaaaaaata tttacgctag gtttttcgac gaagggaatc aatcgtgggt ttggattgaa
    15481 tctcatccat tcaatcgtca aacgccacgg tggattttta gaattttcca aacaaacaaa
    15541 tggcggtaca atcgtctatc tagaattacc aaaaaaggaa gatcaaaatg atgacaaatg
    15601 tcctgataat cgaagatgat ccaatggttg cagaattgaa tcgtactttt gtggaaagca
    15661 tccctaatat gaacgtaatc gaaagtttca gaaatgtaga agaaggaaaa aaatatttca
    15721 gacaaaaaaa cgcagatctg ctgctgcttg atgtatacat gcctggcgaa agtggtatcc
    15781 attttttatc ttggcttaga cagcaaggaa atgatatccc agtgattttg atcactgctg
    15841 cagatgatat cgaaaccata aagaatgtga tccgctacgg tgccttagat tatttgatca
    15901 aaccgttcac tttgcaacgt tttcagctgg cgatcgaaaa gtatcttgct ttctctcaga
    15961 caatccaaac gactgaaaaa gccgatcaga aattgatcga tcaagtattc aaccacacca
    16021 ctcccattca cgaaatacca tcattcatcg atttgcctaa aggtctgtca aaactgactt
    16081 taaaaaaaat aagtgcattt attctaaaag aagatactat tttttcaaca aaagaactgg
    16141 ctcaaaaaag ctcactgtca cgtatatccg tcaagaaata tttgaatttt ttagtagaac
    16201 aagatatatt gaacgaaaga atcaaatatt tggaaatcgg ccggccgcta acctgttatg
    16261 aattgacaaa gaatgggcgg aaaaagctca cagattactg ttgagtcatc gaattatccc
    16321 atacaaaata gtccaagtat acagaagtaa caactgttta cttggactat ttttaaacat
    16381 tttattgacg aatatccatg ttttcttatc ttaattgacg ggctttagga agagatacct
    16441 catttttcgt ttttccagtt tctattattt cacgaaagtt ctcgaaactt gtcgaaatca
    16501 tatttgataa tgcctcatct gtatttgaac ctacatgcgg ggttacaaga actctagggt
    16561 acatttctac taattgctgg atagcagggt ctggaatcgt ctcccatggc ttgaaggatc
    16621 ggaaaaaaac ctctgtttca tttgcaaaaa catcggttgc aaaaccagct aaatgattgc
    16681 ttttcaaagc tcttaaaata gcttcattat cttgaagctc tccacgggct gtattaatca
    16741 agatgctacc ttttttcatt tgagctaaaa atccttcatt gatcatttga tcgttttcac
    16801 ctgggatatg aggtacatga atactgacaa tatcgctttt ggctaataac tcttcaagcg
    16861 agtggaacgt caatactttt ttagcagctt ctgtctgatg aatatcgtag ccgatcactt
    16921 tggctcctag tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct gttcctatga
    16981 tccctactgt gcaatttctg atttcacgac taaacatttg ctcatccact cgaaaatctt
    17041 tgaaagatgt tcggatcgtc gtatacgcag tatttctcaa aagcatcatg gcaaaagtca
    17101 aagataactc tgctatcgca tttggcgaat acgatggtac acgggctact gtcataccaa
    17161 aatcagctgc cgcttgtaga tcgatatgat tgactcccac agtccgtgta aaaacataac
    17221 gaatcccata ttcctgcatt tttgcaagat tggtctcatc tgctatacaa tttcccctca
    17281 acaatacagc atcatggtcc tttgctgaca cgatattgtc atgtgtcaac agttcttcat
    17341 aaagactgag atcataatgg taaatattca atttttcaaa aaaatctatc tcgttcggac
    17401 gaacaccgta acatgcgatt ctcatacttt cttcctccat ttcttatgaa aataaaccta
    17461 tgccatgaat catttgtaat acaacgatcc atacaggtgt cagtaccaca gcaaaaatag
    17521 ttgacagtaa ggatgcattg gaggctaaaa gtgcctccct atcaaaactg atagcatagg
    17581 ctgctgccac tgttgcagct ggtgtagcca tcatgataac gattgttgcc agtgcttcaa
    17641 aattgactgg tagaatattc gttgccgtca aaactgctag aatcacgata ttcaatgcag
    17701 gtacaaatac cactttgact agactgtaat accatgaagt tttttcagat gcagcttttt
    17761 tgaagcttac ttcacctaat gttgcgccga ttgctagcca cgccaatggt gaacttaaac
    17821 cagctaaata atccatcggc tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg
    17881 ctacttggct tatctctcct tctgcacctg taactgacac ttgtggcaga tacccttgga
    17941 acagccaaat gaaaaggccg gcaaaagtag cgataatgat tggattgacg aacatcgctt
    18001 tgatattttt agcttccatc ttcaagccac tcatttttat gtatccataa gaatacaaaa
    18061 agattcggta gccaatattg aaaattgagg aatacatcac gccgacagga ccataaatcg
    18121 cactgacgat aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg
    18181 ttgtttgctt atccccttta tagcttaaga aaaaaggttt tgaaactaga ataagaataa
    18241 tataaataac taatccccaa atcaagacat tgattccttg catcagtaat tctgtattga
    18301 tatcttgcat gaaagctcga aatgccagtg ctggcaaagc cactgttaag acgactttcg
    18361 atagaatttt tccaacagat tcagaaaaaa tccccttttt tctacaaaca aaccctaata
    18421 atataataaa aactgttgaa gtgattgcac tgacaatcga catatctgtc aaagtcgatc
    18481 gaaaaatgtc aaataagttc atctttctcc tcctaattat gttcatttat tcacaataaa
    18541 aaaagctttt attttgctaa tacgtattaa catttttaat tttatcttta cattgttact
    18601 tattgaacaa taggaaatta aagaaacttt tgaaacttat gtaaagtgat ttttactaga
    18661 acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta acttaaatta
    18721 tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt catatcaggg
    18781 ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt atcatcgatt
    18841 ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg tgttgctaat
    18901 ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc agctaagttg
    18961 ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc taaattgtaa
    19021 tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac atgatcatgt
    19081 cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc atcacaaatc
    19141 aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg ttcgcgatga
    19201 ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc ttcaacagct
    19261 ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac tccttcttca
    19321 gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc aatttttttg
    19381 attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg atccccgaaa
    19441 taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc aaaatagatt
    19501 cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc agctacatct
    19561 tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac tccacaagat
    19621 aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac atcatgattc
    19681 atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc gctttggtca
    19741 atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag tccatcggta
    19801 atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc agcatagata
    19861 aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc ttatttagag
    19921 aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt cacagcttgt
    19981 gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta tattaagctt
    20041 ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc ccaatagatt
    20101 ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa gaaatttcca
    20161 taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga ttgatgcagc
    20221 agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc cacaccatat
    20281 atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat tctttctttt
    20341 tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat attttgacgc
    20401 aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt tgcttgatac
    20461 agattttcag aaatatcaga agattttagt acttgttcta acttccgata atcacgtttt
    20521 tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt ttttcgagag
    20581 agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat catcaccaat
    20641 acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga acaaatatca
    20701 tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat gatcgttcca
    20761 aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt cgcattctat
    20821 tcgtattata ctttaggaag tcgcaaactt caagaaaaga cttgcaacta aataatatac
    20881 tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt ctgtggccct
    20941 cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag gaattctctc
    21001 attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat tcgaaaatga
    21061 tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg ttcatgtaaa
    21121 aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc aatttttggg
    21181 aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc attgattgca
    21241 ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag ctcatcgaca
    21301 ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt ggaacacaaa
    21361 cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga cttatctcca
    21421 cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga tagaccgttc
    21481 agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc tagaccatcc
    21541 atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg tcgagctact
    21601 tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt attaggatta
    21661 atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc aatatcagga
    21721 taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc taatgcaata
    21781 gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc attgtccagc
    21841 aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta gatatcattg
    21901 atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt tctagcagaa
    21961 aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat caaatcacga
    22021 atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa
    22081 atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc gcgaacatcg
    22141 taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta gccaatccct
    22201 catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa tatcatactc
    22261 catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca
    22321 agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc aatccattgc
    22381 aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat
    22441 caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct ccatcttaat
    22501 aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt agtgtgttat
    22561 tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg tgagcgttag
    22621 ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat tttgcttcac
    22681 gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt atcctttaca
    22741 aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg gaaacaagct
    22801 actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc
    22861 taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat tataaaggta
    22921 caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac actcatgctt
    22981 ttcattagct ctttaactgc cattagtact aagatggcga taacaacata aagtatatag
    23041 tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa ccttgaacaa
    23101 aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc aaaacttaaa
    23161 taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc tatccaagta
    23221 cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt caggttacta
    23281 ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg acccattgta
    23341 ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat acgcacataa
    23401 tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa taaaaataag
    23461 atattcatgt ctaaaaaaaa cggctttcca aatgatgaaa tacctaaatc taacttatta
    23521 agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc agcacgtaat
    23581 gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat tgaaaatact
    23641 gataacttat aattatatcg tccattgaca atcaaatata aactaactat acttgttaga
    23701 acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa aggaatactt
    23761 acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa cggataaaaa
    23821 gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct ccaaccggtt
    23881 tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa taaattttta
    23941 acatttattt tctcaaaaac at
//
LOCUS       DACDCJ010000045        23721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDCJ010000045 DACDCJ010000000
VERSION     DACDCJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.045"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IV038_001697"
     CDS             299..1960
                     /locus_tag="IV038_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8341903.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IV038_001698"
     CDS             complement(2030..2377)
                     /locus_tag="IV038_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP8341904.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IV038_001699"
     CDS             2461..3402
                     /locus_tag="IV038_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341905.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IV038_001700"
     CDS             3403..4446
                     /locus_tag="IV038_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8341906.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IV038_001701"
     CDS             4452..5522
                     /locus_tag="IV038_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341907.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IV038_001702"
     CDS             5512..6465
                     /locus_tag="IV038_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8341908.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IV038_001703"
     CDS             6477..7487
                     /locus_tag="IV038_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8341909.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IV038_001704"
     CDS             complement(7772..8593)
                     /locus_tag="IV038_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP8341910.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IV038_001705"
     CDS             complement(8690..8968)
                     /locus_tag="IV038_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341911.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IV038_001706"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IV038_001706"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP8341912.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IV038_001707"
     CDS             complement(11724..12311)
                     /locus_tag="IV038_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341913.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IV038_001708"
     CDS             12486..13808
                     /locus_tag="IV038_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8341914.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IV038_001709"
     CDS             13963..14739
                     /locus_tag="IV038_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8341915.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IV038_001710"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IV038_001710"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP8341916.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IV038_001711"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IV038_001711"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP8341917.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IV038_001712"
     CDS             16629..17258
                     /locus_tag="IV038_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341918.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IV038_001713"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IV038_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP8341919.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IV038_001714"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IV038_001714"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP8341920.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IV038_001715"
     CDS             19445..19981
                     /locus_tag="IV038_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP8341921.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IV038_001716"
     CDS             complement(20021..21784)
                     /locus_tag="IV038_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341922.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IV038_001717"
     CDS             complement(21787..23502)
                     /locus_tag="IV038_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341923.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7652 a         4332 c         4808 g         6929 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat t
//
LOCUS       DACDCJ010000046        23576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDCJ010000046 DACDCJ010000000
VERSION     DACDCJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.046"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IV038_001718"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IV038_001718"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP8341924.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IV038_001719"
     CDS             complement(1174..1869)
                     /locus_tag="IV038_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP8341925.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IV038_001720"
     CDS             complement(1882..2598)
                     /locus_tag="IV038_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP8341926.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IV038_001721"
     CDS             2928..3533
                     /locus_tag="IV038_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP8341927.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IV038_001722"
     CDS             3858..4181
                     /locus_tag="IV038_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8341928.1"
                     /translation="MFESFDSSRNRYVSVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IV038_001723"
     CDS             4363..5733
                     /locus_tag="IV038_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP8341929.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRIFQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IV038_001724"
     CDS             complement(5863..7164)
                     /locus_tag="IV038_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8341930.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IV038_001725"
     CDS             complement(7394..9385)
                     /locus_tag="IV038_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP8341931.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IV038_001726"
     CDS             complement(9529..9795)
                     /locus_tag="IV038_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8341932.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IV038_001727"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IV038_001727"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP8341933.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IV038_001728"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IV038_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP8341934.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IV038_001729"
     CDS             13139..13702
                     /locus_tag="IV038_001729"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP8341935.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IV038_001730"
     CDS             complement(13955..14476)
                     /locus_tag="IV038_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP8341936.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IV038_001731"
     CDS             complement(14479..15321)
                     /locus_tag="IV038_001731"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP8341937.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IV038_001732"
     CDS             complement(15314..16039)
                     /locus_tag="IV038_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP8341938.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IV038_001733"
     CDS             16215..16511
                     /locus_tag="IV038_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341939.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17079..18671
                     /locus_tag="IV038_001734"
     CDS             17079..18671
                     /locus_tag="IV038_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8341940.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18684..19424
                     /locus_tag="IV038_001735"
     CDS             18684..19424
                     /locus_tag="IV038_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8341941.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19592..20941
                     /locus_tag="IV038_001736"
     CDS             19592..20941
                     /locus_tag="IV038_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8341942.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21069..21659)
                     /locus_tag="IV038_001737"
     CDS             complement(21069..21659)
                     /locus_tag="IV038_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8341943.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21806..23158
                     /gene="brnQ"
                     /locus_tag="IV038_001738"
     CDS             21806..23158
                     /gene="brnQ"
                     /locus_tag="IV038_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8341944.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6754 a         4745 c         4490 g         7587 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgtttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatctttc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgac acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacaggaaa ttttctgttc tagttctttt
    16561 tttattgtat tacatacatt tttgtgtata attatactat cgtttgcaat ggatagatga
    16621 ttcacatttt gattgttttt tcggtaaaag taaagatttc tatatttatc agtatttttt
    16681 caaaaaccag cagttataga ttgacaaagc ctctcgagta tcgtaaactt tttttgtata
    16741 aataactgaa tacgagatgc ctcaatcaat gaggtagagg tcgcgagaat cattaaccct
    16801 gtggagtgaa gcaaacatgg agaagcagga atagggagga tcgccgaaat gtgcactttt
    16861 ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc ggactgtctt agcagtgatg
    16921 ctaagttgcg ctatgttttg tgagaggttt atcaccttat ggaattttag cggccttttt
    16981 atatctataa atcggtcgct ttttattttt tcagataaag ctttaccgca tttcatctag
    17041 accgctcgtg tatcatataa tcagaaaggg agaaaaaaat gaaaaaaaat atggtttcag
    17101 ttgccgtact atttgctttt gtcttctcac tattcagtgt gggatctctg gcaaatgcag
    17161 atgaaaaaac accgagcgat gagtttcgcg taggtatgga ggctgggtat gcacccttca
    17221 actggtctca acaaacagat gagaataatg ccttgccaat tcaaggccaa aatagttacg
    17281 ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga tggtttaggg aaaaaattag
    17341 tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct tcaatcaggc aagatcgacg
    17401 cgatcatcgc cggaatgagc cctactgctg aacgacgaaa agaaattgat tttaccgatc
    17461 cttattacga atcacaatta gtagtagtag ttcaaaaaga tagcaaatat gcaaaagcaa
    17521 aaagtttagc agatctttct ggagcaaaga tcactgctca gttaaataca ttccactata
    17581 ctgtgatcga tcagatacca gatgtgcaga aacaacaagc aatggataat ttctcagcaa
    17641 tgagaacagc tttagcttct ggaatgatcg atggttatgt cagcgaacgt ccggaaggtg
    17701 taacggcaac tagtgtgaac aaagacttgg aaatgcttga attcagcggt gcagatggct
    17761 tccagacaaa cccggaagat gttcagattt ctgttggtat gcgtaaaggc gacccggata
    17821 tccaaaaagt aaaccagatt ctttctggca tctctaacga tgaacgaacc aaaattatgg
    17881 accaagcagt caaagatcag cctgctgcaa ctgatagtga agaaaaacct ggcatcattt
    17941 ctgatttcac tacgatttgg aaacaatacg gcaatatgtt cttacgaggt gctggactta
    18001 ccttgttcat cgcattgatc gggacagttg tcggtactac acttggatta ctgattggtg
    18061 tcttccgttc ggtacctgaa tcaggaaatg cactgactcg cttcttccaa aaaatcggaa
    18121 atgcacttct ttctatctat gtcgaagtat tccgcggaac accgatgatg gtacaagcaa
    18181 tggtcatctt ctacggtctg gcactcgctt ttggcatttc attgaaccga acgattgccg
    18241 ctttgtttat tgtctctctc aatactggag cttacatgac agagatcgtt cgtgggggga
    18301 tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc cattggtatg acccatggcc
    18361 aaacgatgag aaaagttgtt attccacaag ttttacggaa tatcttgcca gcaacaggaa
    18421 atgagtttgt tatcaatatc aaagatacag cagttttaag tgtaatcggc gtagctgatt
    18481 tgttcttcca aggaaactct gcttccggtg cgaatttcca attcttccaa acatttacga
    18541 tcgtcgggat catctactta gtcatgacct ttacgatcac cagaatcctg cgggcggtag
    18601 aaaagaaaat cgatggtcct tcagcttata ccaagcttga agaaatcgac aattcaaatt
    18661 tgcaggaata aggaggaaga atcatgaatg aaaaaattat tgaaatcaaa catctaagaa
    18721 aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt aacagtcaat aaaggtgaag
    18781 ttgtgacgat catcggttct tccggttctg gtaaatctac tttcttgcgc tgtatcaatt
    18841 tactggaaaa accaacaggc ggcgagatca tttataatgg agaaaatgta ttagctccta
    18901 aatataattt acctaaatat cgaacgaatc taggtatggt tttccaatcc ttcaacctgt
    18961 tcaacaatat gaatgtcttg gaaaactgta tgtctggtca aacaactgtc ctgaaacgag
    19021 ataaagaaac tgccaaaaaa gtagcaatcg aaaatctgga aaaagttggt atggatcgtt
    19081 atatcgaagc caaaccagca caattatctg gtggacagaa acagcgtgtc gcaattgctc
    19141 gggcattatc aatgaatcca gatgtattgc tattcgacga acctacttcg gcgttagacc
    19201 ctgaaatggt tggcgaagtg cttaatacga tcaaagactt agcacatact ggcttgacaa
    19261 tgcttatcgt gacgcatgag atggaatttg caaaagatgt atctgaccgt gtcattttta
    19321 tggataaagg tgtgattgca gaagaaggta caccagaaga tatctttgtc catccgaaag
    19381 aagaacgtac aaaagaattc ctttcacgta ttttaaatgc ttaggatcaa tagctgattg
    19441 ggacaaagag ctgcggtgtt taccgcagtt cttttttatg aattactcat aaaaggtaaa
    19501 tttctgaaaa ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta
    19561 attaaacttt tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg
    19621 actgaaaaat agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt
    19681 tttaggttcc ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat
    19741 tactggatgc atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac
    19801 caataaccat tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat
    19861 cactggctgg acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat
    19921 cggaatgtat gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc
    19981 attaggtctt cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt
    20041 ttggtttgca ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat
    20101 gatcctcacc cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca
    20161 tggcggattc tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt
    20221 tgcttttgct gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa
    20281 agtgttgccg gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc
    20341 tctttttgtc attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt
    20401 tgtagaagta ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt
    20461 attaagtgca gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg
    20521 ttctttagcg caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt
    20581 gccagggaat gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta
    20641 cgtgatgcct agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt
    20701 tatctggagt atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa
    20761 agctcattca tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat
    20821 ggcctttgtg tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac
    20881 acctgtgtgg ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta
    20941 agaaagtagt tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt
    21001 gcttcttaaa tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag
    21061 cctcttttct atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa
    21121 aaaatccaaa taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag
    21181 acaccggcaa tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac
    21241 aaaactctca cgacatggga atctggttta tttgctttca tgatttgcct catcaagata
    21301 ccaaaagaaa aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct
    21361 tcagggttac gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa
    21421 aagatattcg accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga
    21481 tggttcaccc agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt
    21541 cggatgaccc ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga
    21601 actctcaaaa caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg
    21661 atgattcctc ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca
    21721 tcgttttata tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt
    21781 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    21841 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    21901 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    21961 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    22021 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    22081 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    22141 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    22201 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    22261 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    22321 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    22381 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    22441 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    22501 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    22561 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    22621 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    22681 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    22741 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    22801 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    22861 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    22921 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    22981 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    23041 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    23101 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    23161 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    23221 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    23281 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    23341 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    23401 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    23461 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    23521 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaaaaa
//
LOCUS       DACDCJ010000047        23330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDCJ010000047 DACDCJ010000000
VERSION     DACDCJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.047"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(8..2152)
                     /locus_tag="IV038_001739"
     CDS             complement(8..2152)
                     /locus_tag="IV038_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8341945.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFNHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2509..3882)
                     /locus_tag="IV038_001740"
     CDS             complement(2509..3882)
                     /locus_tag="IV038_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8341946.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3882..4277)
                     /gene="rbsD"
                     /locus_tag="IV038_001741"
     CDS             complement(3882..4277)
                     /gene="rbsD"
                     /locus_tag="IV038_001741"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP8341947.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4305..5222)
                     /gene="rbsK"
                     /locus_tag="IV038_001742"
     CDS             complement(4305..5222)
                     /gene="rbsK"
                     /locus_tag="IV038_001742"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP8341948.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5330..6343)
                     /locus_tag="IV038_001743"
     CDS             complement(5330..6343)
                     /locus_tag="IV038_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8341949.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6424..7023)
                     /locus_tag="IV038_001744"
     CDS             complement(6424..7023)
                     /locus_tag="IV038_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8341950.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7209..7910
                     /locus_tag="IV038_001745"
     CDS             7209..7910
                     /locus_tag="IV038_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8341951.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7920..10631
                     /locus_tag="IV038_001746"
     CDS             7920..10631
                     /locus_tag="IV038_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP8341952.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11082..12710
                     /gene="treC"
                     /locus_tag="IV038_001747"
     CDS             11082..12710
                     /gene="treC"
                     /locus_tag="IV038_001747"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP8341953.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12770..13951)
                     /locus_tag="IV038_001748"
     CDS             complement(12770..13951)
                     /locus_tag="IV038_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8341954.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDYTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14203..15726
                     /locus_tag="IV038_001749"
     CDS             14203..15726
                     /locus_tag="IV038_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8341955.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15795..16523)
                     /locus_tag="IV038_001750"
     CDS             complement(15795..16523)
                     /locus_tag="IV038_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8341956.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16536..17816)
                     /locus_tag="IV038_001751"
     CDS             complement(16536..17816)
                     /locus_tag="IV038_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8341957.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17957..18559)
                     /locus_tag="IV038_001752"
     CDS             complement(17957..18559)
                     /locus_tag="IV038_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP8341958.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18804..19010
                     /locus_tag="IV038_001753"
     CDS             18804..19010
                     /locus_tag="IV038_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341959.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19322..20041
                     /locus_tag="IV038_001754"
     CDS             19322..20041
                     /locus_tag="IV038_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP8341960.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20122..20805)
                     /locus_tag="IV038_001755"
     CDS             complement(20122..20805)
                     /locus_tag="IV038_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP8341961.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21023..23209
                     /locus_tag="IV038_001756"
     CDS             21023..23209
                     /locus_tag="IV038_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP8341962.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7766 a         4202 c         4569 g         6793 t
ORIGIN      
        1 atttattcta tttctttaaa ggcagatcaa gccagaatga tttgtctaaa cttaacaagg
       61 ttagtacagg aacagttgca gctttaactg tacgagtagt tgtttctggt atctgtcttg
      121 ccgtaaaatt gttccacaaa tcccagattg aagcgacacg gaagatggaa gaagcgttgt
      181 tgacgcgttt tatcgttgaa aattctaatc ctgtgattgg cacgccatct gttgtccatt
      241 tgattggcac atcgaatcgc ttagacatat tgaggatttc agcgtttgct tcttcagttg
      301 ttgctgcttc ttttttcggt tcatcatatt ttgtcaggtc gtacgcttcg atgatcgcgt
      361 tcaatttttg tgaatattgg cgatctgtag cataacgtcc ttccaaatat ttggttgctt
      421 ctttgtagtc ttttgtttcc gacttccaag ttggtttgta gaaatcagga ttgccagtca
      481 gtccattttt gatcaagtcg gcatagtctt ccaaactttc cttgtaagaa ggatatttac
      541 ggaattgtgc gcggatttgg tagctttgtc cggaactatc ttgttcaagt gtattgaagg
      601 tcacgctgct tcctttatat gatcctttga taccaaaaag gttatgattt ggttcgcttg
      661 ataataagct gtttccagat ccactttcaa ggatagcttg agccatcatg actgatgcgt
      721 agattccttc tttttgcccg atttcctgtg catcatctgc aatctttttc aagaactgat
      781 cagttgtttg gttttgactg aaatggaacg agccatctaa tccatctaaa atagatgagc
      841 cgcctgtcag cgaacctgaa gcagcaggta aacctgctaa gtttactcgg acagcaaaat
      901 caggcgtgaa atctgaaata gaagttactc gaactttatc tcctggttga ggcgcatgga
      961 taaattgtcc gttcccaata tagataccta catggtgagt aacaccggaa gaaggtcccc
     1021 agaagacgag gtcgccaggt tgggcctgac ttactggaat ctgcgtacct gcatattgct
     1081 gttcgcctgt ccagccaccg atataatgac cggttgcttt catatatacg taataagtca
     1141 aaccagaaca gtcaaaagta tttggacctt ttgctcccca tacatatggt tttccaagat
     1201 gattgaatgc ttctcgaacg attgcttgtt ggacattcgt tgtatttgta ggcaatacag
     1261 catcaattgg tgattttaca acaaaatcag aagaagcaga agatggattc tctataccgc
     1321 tttgatttgc tgtatttgga cgagatgaag cattgttcgt tccgccattc gatgaaccat
     1381 tgtttggatg gtttggttct ctctgagtat ttccattgtt attcgatcca gatgggtttg
     1441 tttgattcga agaattcccg ctgttcgaat tattgttatt attgtttgaa ttatttgaac
     1501 cattattatt gttgttatta cccacatctg gtttgtttgg cgtttccgga tctggtgtac
     1561 tagggacttc ttcactagga tcagttggtt tctctgttga agtatccggt ccagctttaa
     1621 attctgcagt aatagataaa ttgttcgtac ctactgtaaa ggtctttgca tcataattta
     1681 cttctttggc accattaaca gcgactgaag caatttgttc accttcagct ggcgtaatag
     1741 tgtaagttac cttcgtttcc gctaataaac cttctaccgt tccactttcc gtaatatcca
     1801 aaggttgctg ttcaggggaa tccgctgtcg aagcagtcag ttggatctta ccaatcaaag
     1861 aaggagcaat tgtaagggaa tatgtctcgg ctggcttttc attttctgat ggatcagccg
     1921 gatcaatttc agttggctcg gtcgatcctg ttgttgagtt agttgttcca gttgggtctt
     1981 ctgtaaaaga atccgttgtt tcactagtag ctgagctgga tgtagtcgta tctgttgtga
     2041 ctgtttgatc agctatttga ttatctacgg caaatacggt attggtcgac aaaacagacg
     2101 gagcaatcag ccaagtagca gctagcaaac gtatcgtttt tttatttttc ataataaact
     2161 agtccttttc ctttcattga ttttctgtct tattcagcat tcattagaca ttttttggaa
     2221 cagataatcg tgtcgaaaag ctttttcttt tcataaatct gtctgtgtat tctaaataaa
     2281 acaatttgta aataagcaac gagcattcat tcgtttttct ttgacgttca ttttattgca
     2341 tagaccacct ttgtttgggg ataaacaagt ggggataaaa atggttatgc atatgtaaat
     2401 tataccaaag tttggaaagt atcacaaaaa tttataaaaa taatgatcct aaaaagaaaa
     2461 agactgaaat atgtgataaa catctcatac ttcagtcttt ttcattaact attcataaga
     2521 cattaggtct tcatcaaacc cgctaacgag agtagtgatc attgctacga ctagactaac
     2581 agccagtcct gctaagtacc agaaaaaagt accggaaatg aatgcaggca atgtggtcag
     2641 actaccaaat acaaaagcga tcgctttcac ttgggcaaat cccattacag ctccaccagc
     2701 agctgcacca atgcaagcag caacaaatgg ttttttcaat gggatcgcaa ttccgtaaac
     2761 agttggttcg gtcactcccg caagtaaaat cgaagcaaaa gcagaaccgg agatcgaacg
     2821 ggtttttgaa cgtttacttc gcagaaatac acctaaagct gcaccagcca ttccaaaatt
     2881 ggctgctcct gtcaacggaa cgattcggtc gaacccataa gcagagatat tactgatcat
     2941 gacagggttt acagcccatt ggataccaaa tattaccaaa atattccaac caccagcgat
     3001 gattgcgcca ctgatcggtc cgttaatatc catcatccaa gtaattgcgg cagctaaacc
     3061 ttcgccaagg taaacacccg caggcccgaa taccattaac gtcaaaggaa ccgtgataac
     3121 taatgaaagt aaaggtacaa atactaattg catattttcg cgaatatatt ttttgaggaa
     3181 tttttcaaca aaagaataaa agaaaatacc taaaatcgcc ggcatgacag tagacgtata
     3241 ctgcatcagt actacaggaa tccccagaaa atcagtagct gctttgccac tgtctgaaag
     3301 taaagaggtc agattcggtt caatcagtgc tgcagcaatg acagccgcaa tatatccatt
     3361 cactttaaac gttttggcag cagaaaaagc aagaaagatt ggcagaaaat aaaagacggc
     3421 attcgacgca gcagacaaaa tatgataagt tccgcttgtt tcactaagcc agccaatcgt
     3481 agtcgctaaa acaaccagac cttttaatat accagaaccg gcaaacagcg gaatgatcgg
     3541 cgtaaagatt tccgatatca aaccaaatag tttttcgatc ggatttagct tttctttcgg
     3601 agctgaatga tcgatttgac gcgtcaattc cttgtaagca ggagccaatc cctgatccat
     3661 gattatttct gcatatctat cgagtaaaat gaaatactta atggaaggga tttgctgttt
     3721 taatttttct gcattggcta ctggctgttt tttcaatttg atacgaagcc gagtcgcaca
     3781 tggaaggaca gaatcaatat tgtcttttcc tcccaactgt tcaatcagca tatgaatctg
     3841 ctcggacagt accatctgtt caggattcat tgtcgtttcc attagaagac cactccactc
     3901 actaatataa tattggcgta aggtgtcatt tcgcctgtac gaataaatgc atgacagtta
     3961 ttcaactctt ttttcatttc ttcatgtggg ataaagctaa tcggtgtatc tggtaacaat
     4021 aatttgattt tttccaatat ttccgggttc attgtcttta tttcttccgc caattcaatc
     4081 gattgtacct caagttcttc taaaacgttt tctaaaacag ataaaaaact tggaagaccg
     4141 tttgagacag ccaaatctat tttttcagta ccaaagggga caggcatgcc tgcatccccg
     4201 atacttaatt tatcaaagtg ccccatttgt gcgattactc gtgaaatatc tgaattgatt
     4261 acttttcctt ttttcatgtt tgtttcttcc tctcattgtt aattttagac tgctgattgt
     4321 tctaaagctt cgcggtaagg gatagaaggt tgcgcaccat aagactgcac agcaacagaa
     4381 gaggctagat ttccataaag gatcgcttct ttcaagttac taaggtctgg ctgaagtgtg
     4441 ctgcttaatg cgccaataaa tgtatcacct gcggctgttg tgtcaacggc atccacttta
     4501 aaagcaggga cgataccgtg ttctttttct gtatgataga aagcgccggc actgcctagt
     4561 gtaatgatta ctgttccaat acctaattca tggagttttt cggctgctgc gaccaaactg
     4621 ttatgatctg tcactctgat tcctgtaatg atttctgttt ctgtttcatt tggtataatg
     4681 atatctgtct tttcaagtag ttcagtagga atcgtttcac gagctggtgc aggattcaag
     4741 atcgttgttt ttcctgcgtc ttttgcaata gaaaaagcta ggatcgtcgc atctaagcta
     4801 ctttcaaact gagcaataat aaagtcactg tttgtaatca gttgcttgtt ttttaggact
     4861 tcttgttcat ggaaagcatt atttgcccct gcatggatca agatactatt ttctcctgct
     4921 gcgtctacca taatcattgc ttgtccggtt gtggcgcctt ggatttcagc tatcccagat
     4981 gtatcgatgt tttcttttgt cagtagatct aataactgtt gtccttcgct gtcagcccca
     5041 acgcctccaa taaaagatgt ccgtgcgcct gaacgtttgg ctgccactgc ttggttggca
     5101 cctttacctc cgccagcgtg aaaaatctca tgcgcatgca tcgtttcgcc tggcttgggc
     5161 atgttggtta aacgaagggt agtgtccata ttaatacttc caattactgt aaccgtgttc
     5221 attttacaaa cctccaacta tctcatttat tttgatggga aaattatttt ttaaaacgtt
     5281 ttagtaaaac gttttaatag ttcgataaaa atatagcaca ccttgaaagc tatttcaagg
     5341 gtgcagttga aaatcttttt tctaatcgta caggtaatct tttttcttcc cattcttttt
     5401 ctgggaattg gatccgatct aacaataact tggcagatgt ttgtcccaat tcgaagattg
     5461 gttgtgcgat cgttgtcagt ttcggcttga tatactcgca catatctata ttatcatagc
     5521 caataatgct gtagtcttca gggatgcttt ttccagcttc ctctaatccg cgatataatc
     5581 caaaagctaa ttcatcattt aaagcaaaca caccagttgc agcagaatca agaagttctg
     5641 ctgtgatctg gtagccacct tgttttgaga actgtgtagg taagaggatc agttggtcgt
     5701 gagggatttg gtaaacatca agagcagatt taaacccctc aatccgagcg tggacatttt
     5761 ccggtggatt ttcaggatag acaagggcga tcgtttgatg tcctagacta agcaagtgca
     5821 tgcccgctag gtaaccaccg cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag
     5881 attttttctg atccaaaaca atgaagggcc ggccttgttt tttcaaattt tcgttgattg
     5941 catctgtaga gacagcagaa gtcgcgataa tgaatccatc cacacctcga cgtgtgagct
     6001 cggctagata ttcgatttct ttttgatgat cagaatcagc attacataat atcgtgacga
     6061 aattctgctt atacaagata tcctcaattc ctcgcatcag tgtactgaag aatggattcg
     6121 tgatatcagg aacgagtaca ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa
     6181 aatagtcagg ctgatagcct aattcttctt tggcagctaa aactttctga acagttttcg
     6241 gactgaattt ctccgtattt ccatttaata tcaaggagac agttgtgatt gaaacgccgg
     6301 aaagtttagc gacttctttg attgtgatct tttttttcac catgttcata ctcctatcta
     6361 aatcctgtgg ttattttccc atgaaatcaa aaagaagtaa atgaaaatca cgtgtgccag
     6421 tacttatttg ctttgttgga tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt
     6481 tttcggaaat atctttttta tgaacaattg atgggattct ttgtcacgat cactgcaagc
     6541 atcttcagca agcacgatat ggtacccgtg ttgaaaggca tctaatgcag tagcatatac
     6601 accgtttgtt gtcgctacac cgcttaaaat gatagtttcg attccacgcc ggcgaagttg
     6661 aagatcaaga gaagtaccaa aaaaagcact tggattgtat tttgtgatag tcaacatatt
     6721 atctgtatct ttcaaagaat ccttcaaaag taaatcagtg aattctggag gtacagaagc
     6781 agtcattcca cccatatcag tttgttgact caaataaggg aaagtggaga tgtctacgtt
     6841 cactaaaact ttcaaggcag cggtacgttt caatgttctt actaacaaat cattgtttgt
     6901 cagaatagat gctgcagtgt gtggagagag tgtaccgtta tttacgatct ctttttgcat
     6961 gtcgatcgcg acaaaagcag tttttgataa atcaaaaatc atattttcct ttaaatcatt
     7021 catgatgttc cttctttcaa ataacaatgt tttttatgta agtaaatcat aaatcgttta
     7081 tttgtcaact aaattaaagg attcctttct ttagtctaga gaaattcagt ggtaaaataa
     7141 ataagtgtga gaaaacgcat tcgatgcata gatatagcga gaaatatcgt gaaaatggag
     7201 gaatcagagt gaagtatgtc gaagtaaaaa atgaatacaa acgatacaaa atgggagaaa
     7261 cgaccattac agcaaatgac gatatttctt tttcaattga aaaaggcgaa ttagtgatta
     7321 tacttggtcc aagtggagca ggaaagtcaa cgattcttaa tattttaggt gggatggata
     7381 cacctgatga aggtcagatc attattgatg acacagatat tgcgcaattt tcagataaac
     7441 agcttactgc ttaccgacga acagatgtag ggttcgtttt ccaattctac aatctcgttc
     7501 ctaatctaac agcaaaagaa aatgtggaat tggcaacgga agtttctcct gatgcgcttg
     7561 atcctgtaga ggtattgagg caagtgggcc tagctcatcg attgaataac tttccgtccc
     7621 aattatcagg aggcgaacag cagagggttt caattgctcg tgcactagct aaaaatccca
     7681 aacttctttt atgtgatgaa ccgacaggcg cgctggattt tgaaacgggc aagcaagtat
     7741 tgaaattatt gcaaaacgct agcagacaac acggcaatac cgttttgatc attacacata
     7801 attctgcttt agcgcccatc gctgatagag tcattcacat taatgatgct aaagtaaggt
     7861 ctgttgaatt aaatgatcat ccttcatcca ttgatgagat tgtatggtag ggggatttta
     7921 tgaaaacaag aacatatctt aaagcaggtt ttcgcgaaat tcggcaatct aaaggacgat
     7981 tcattgcaat catccttatt gtcatgctag gaacactttt atttgtaggt gtcaaaactg
     8041 ctggacctgt catgcaaaaa acgatggatc actatgttgg tacagcagga ctttctgatt
     8101 tgcaaatcgt ttctactggt ggcttgacac aaaaagatat tgctcaagca gaaaagatcc
     8161 cagatgcaaa agtagaaaca ggaaaacaga tctattattc caatactggc aaaaacgaag
     8221 tgattcaaat attttcttat aacaaaaata acaagcagaa caagctacaa ttaactgatg
     8281 gaaaactccc ggaaaaacca aaccagcttg tattggatga aaaagcggaa gatgaagggt
     8341 ataaaatcgg tgatatttat cagattgatt cagatgaatt gaaagaaaaa gagtatacca
     8401 tcacgggctt tgtccgttct ccattgttca tcaacaattt ggagagagga tacgcgaacg
     8461 tcggaaatgg tagcgtcgat tattttgtct atttacctga atccgttttt aaaacagata
     8521 tttattctgt cttatatctt gattttacaa acgtaaagaa tttgaatacg tacagcaaag
     8581 cttacaaaga taaaatggag caaaatcaag aaaaggcaga aaagtattta caagatcgac
     8641 ccgaagaacg cttagaagag ctgaaaaaat cggccaacga agaattagaa ccggcacagc
     8701 aaaaaatagc ggatggcaaa gcacaaacaa cgcaagcaag aacacggtta gaagatgcta
     8761 aaaaacagtt agcgcaacag tcagcaacta tccagcagct tcctgaagcg caacgaacaa
     8821 tcgctgttca aacattatct caacaagaag ctcaacttgc tgaacaagaa aggcaattag
     8881 ttgagaaaga acgtgaactg gcctctgcag aggaagaatt gaatcgaaat gcagaaaaaa
     8941 ttgaaaattt aactaaacca tcatacctat acaatgaacg ttcagaaaat gtcggttttc
     9001 aagaatttgg tgatctgtct gatcgtatcg ctgcgattgc caatgttttt cctgttttct
     9061 ttttctttat tgccgcattg atcaccttta cgacaatgac acgtatggta gaagaaaacc
     9121 gcagggaaat cgggacatta aaggcgttag gttatacgaa agttgaaatc gcaaaaaaat
     9181 atgcaatcta tgcaagtcta gcctcaggga taggtattgt tttaggaacg atattaggaa
     9241 cgaatctttt gccaaggatc atttttgagc tgtctaacga gcggtatgat attgggagtg
     9301 cgcttatttt ttatgactgg ccgccgattt tacaagcagc tgccgcattc ttcattgccg
     9361 catttggcgc tgcaatgatc gttttattca aagatttaag agaacggcct gctgcattgc
     9421 ttcaaccgaa agcaccgaag ccagggaaac gcatttttct tgaatacatc actccgctgt
     9481 ggtcacgcct tagctttaat caaaaaatca gttaccgaaa tctcttccga tataagtcaa
     9541 gaatgcttat ggcgattatc ggaattgctg gatgtgccgg gctgatggtt gcaggcgtgg
     9601 gattgaaaga ttctctaagt tctgtttcag ccaaacaatt tggaacgatc attgactatc
     9661 aagcaatcgt tacattggat tcatccacgg aagatagtcg tcaaaaagca gcggatatgc
     9721 tgcagaagca agaaaaagta aatgagatcc tagctattca tactcaaacc gtagaattga
     9781 gaaaaaaagg acatgcttcg caaagtctga cagtgatcgt accaagtaaa acagctgatt
     9841 tggatcgttt tatccattta actgatcttg aagagaaccc acgctccttg cctaaaacag
     9901 gaatcgctat tacgcaaaaa gtagcagaac tatttgactt atctacagga gatcatattt
     9961 cgctttatga tgaagatcaa aaagaatgga aagtcgaagt aaaagaaatc gtgaaaaact
    10021 atctaggtag ctttatttat atgtcccctg aatattatgc ttcagttact gatcaatcga
    10081 tgatggataa tgcatttttg ctgcgtacag ataagatgac aacaaaagaa gaagaaatgc
    10141 tttccgagaa actgcttgcc acagacgctg tgacaaatac gtctttcatg tccaaacaaa
    10201 tcgaaacgca agaagaatca atggcgaact tagactcggt tgtcttaatc tttgtcgtat
    10261 tatcaggact gctggctttt atcgtcttgt ataatctaac taatatcaat atttccgagc
    10321 gtgtacgcga attatctaca ataaaagtcc ttggattttt tgataaagaa gtcacgatgt
    10381 acattgttcg agaaaatatc atctttactc ttttaggaat catcggagga tttggtgtag
    10441 gatatatatt gactgacttc atcttgcaac aagcgtctat ggagaatgtc atctttccat
    10501 tagtgatcac atggtgggct tatgtcttat cagcaggatt aactatcgta tttacggtta
    10561 ttgtgatgat tgttacccat ttcaaattaa agcatatcga tatgattgat gcgttgaaat
    10621 cgaatgaata aaaacagagg ttgtgaaatc agcggtttga cctgagtatt agagaaacaa
    10681 acggcaaaat agtttttcat attttgccgt tttttgagct aataccgaag gacagctgat
    10741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    10801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    10861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    10921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    10981 taaattttac aaacgctttc ggttcagtta taataataaa tgataacttg tatatataag
    11041 ttgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    11101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    11161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    11221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    11281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    11341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    11401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    11461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    11521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    11581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    11641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    11701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    11761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    11821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    11881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    11941 attttgcaga attaaagcga ctatttcata catggggaga aaagatgagt gagggtaacg
    12001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    12061 ctatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    12121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    12181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    12241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    12301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    12361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    12421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    12481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    12541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    12601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    12661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    12721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    12781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    12841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    12901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    12961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    13021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtatagt
    13081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    13141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    13201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    13261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    13321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    13381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    13441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    13501 cgtcaataga agcgaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    13561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    13621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    13681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    13741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    13801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    13861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    13921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    13981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    14041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    14101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    14161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    14221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    14281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    14341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    14401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    14461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    14521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    14581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    14641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    14701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    14761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    14821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    14881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    14941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    15001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    15061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    15121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    15181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    15241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    15301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    15361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    15421 tttacgttta agaatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    15481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    15541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    15601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    15661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    15721 cactgatagg caaaaaagaa ccgaagggtt tggatcggct ggtcatttag tcggtccaaa
    15781 ctattcggtt ctttttatga aatcaatttc tttaatccac ggatagaggc tgggatggtc
    15841 tcgccgtttg ccaacgtgac ttctctggtt tttttattaa tcgaccgcac attttgtgta
    15901 ttgataacat aggatttatg acaacggata aaattccgat gcattttctc gatttctttg
    15961 atcttctcat agaattgtaa ttgggcatga gtcaaatgga cttccagttt atgtggcgct
    16021 cctagcgaag tagaaaaata ttggatgtct ttgatcggta aaaatttgat ctcatcatta
    16081 aactctattt tgaagatatc tttttggatt tgggacattt gaaaatcttc gaaaattttg
    16141 ctcaggatat gctcgacatt ttcttttaaa tcataaagat cttctttgac gatataatca
    16201 gtcggttcga tgttgctttt taagattttc atcgacattt ccgtatggga agtcacatag
    16261 attatttttc ctaaaggatc taattcacgg atcaatttac cgatcgctac accatccatt
    16321 tcactgtctt ctagttcgat atctaaaaag taaataccaa aagaagaagt tctgatcttg
    16381 gctgcattga gaagttcttc cggatcagag gtagaaatat ggacataact gtctaagttt
    16441 tcaatcatga ttctattttt gatcaccgtt gataaaactt ctaattgttt tggatcgtct
    16501 tcacaaatat aaatgggaat catatcatta ctcctttatt ttatttgtat ataaatcgtt
    16561 tgagaaaaac gctggttttc aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc
    16621 aattcatcca agataaacaa gccaaggcct tcgttttttg gatttgtttt tgttgagaac
    16681 cctttttgtt tcaactggtt taacggtaat gtatttgttg ttgtattgat cacacgaatc
    16741 atcacggaat gattcttctt gagtatggtc aattcgattt ttttcttctt tgcagcacta
    16801 gctccttcaa tcgcattgtc caagataata ccaagcatac gtacaaggtt taccgtatgt
    16861 ttcgtatcca gttcgattgg ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt
    16921 gccatcatga gttttaatga cagtaaacta cggatctcag gaactttcat attatccaac
    16981 atacttagat tggccggaaa aaggttcatc atctggcgtg taggtaatat cgtttgatca
    17041 taataagacc gtaatccttc taagtcgcct tctgaaatat agctgtgaag agaaaaaagc
    17101 agattttggt aatcatgttt gaattctctc aagtcttgat agtttttttc tagatctttg
    17161 atataatcct gttgcatcct gacttgttgc tctaataact gatcagctaa tttcttatta
    17221 gagattttcc aataataaac caagatgatc aggccaacac acagatatag aatgaaaaaa
    17281 ctcgtattca aagctaagac atcaggtgtt tcaccagaaa aacgggtata aaaaataatg
    17341 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
    17401 tgaaaccaag tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg
    17461 gataagacaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
    17521 acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
    17581 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
    17641 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
    17701 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
    17761 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
    17821 tacagctccc attgattttt accaaattat agcacaattg gcgtattatt tttttaattt
    17881 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
    17941 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
    18001 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
    18061 tagaaaagta agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga
    18121 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
    18181 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
    18241 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
    18301 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
    18361 agatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
    18421 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt
    18481 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
    18541 tcacaacttt atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat
    18601 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta
    18661 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
    18721 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta
    18781 taaaaggtga tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat
    18841 atgcatttaa atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg
    18901 aaaatagtgt gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata
    18961 atctgcactg gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa
    19021 aatgttgaga gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa
    19081 tgttaaaaaa cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg
    19141 agaatactgt ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc
    19201 atgaatagga gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt
    19261 caaggctttt atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag
    19321 gatgccgata attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac
    19381 tgaaatgctt gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga
    19441 agtattgccg ctattttatt ctaatccaga aaagtatact tttttattac agattttctt
    19501 tttgaacaaa cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga
    19561 ccgctccatc tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc
    19621 tgaagtagaa gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga
    19681 aatttcgcca caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat
    19741 gttagcgaga atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact
    19801 ctattcctac tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat
    19861 atgtcccaaa atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg
    19921 agaacaaatc gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc
    19981 ccagtcgatc cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata
    20041 atataaaaac taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt
    20101 agtttttttg attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa
    20161 atgaaatgtc tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg
    20221 ggcgccaaac agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac
    20281 gacacttaga tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc
    20341 atataagttc gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg
    20401 cccgttgatt ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt
    20461 tactaatgcg ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc
    20521 ataatataga acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag
    20581 atactttgtt agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac
    20641 acttagccat tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc
    20701 agaggcaaaa gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt
    20761 cagcaaaagt cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt
    20821 ttatgaatat ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt
    20881 cagaaaacta aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa
    20941 gaaaaaatag acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa
    21001 agatgggaag gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt
    21061 gaaacgctgg gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat
    21121 cattgctacc tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca
    21181 agtaaaagaa agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa
    21241 tcgcttcaat caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat
    21301 catagcagga gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat
    21361 gacgcaaaaa caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc
    21421 aaatgaacta aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg
    21481 aacattccat agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt
    21541 agcaatgcaa ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt
    21601 tttgcaacag ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac
    21661 gattgcttct cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca
    21721 gttagttaaa gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg
    21781 actgtatgga ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt
    21841 caatgatgta ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat
    21901 cggccaaaaa tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg
    21961 ggaaatcgtg aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga
    22021 tgatcagagt atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa
    22081 agaatatcct accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt
    22141 ccaaggagca aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat
    22201 tccagcacaa aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga
    22261 aagtcagtgg ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga
    22321 atcaatcata ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa
    22381 aacgaatatt ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa
    22441 gtctttccaa catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa
    22501 gaaaaatcag gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa
    22561 aagtgaatgc gatcaatact tttctcctaa atatcaaaat atatccgttg cagattatat
    22621 tttgtcgata agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc
    22681 gttgcgtaaa acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct
    22741 gccacgtata gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa
    22801 gcagcatgct actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa
    22861 agaattaaaa acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca
    22921 gagcattcac aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga
    22981 aggaagtttg ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa
    23041 agcagaacca gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag
    23101 agcaagaaat cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt
    23161 atcgagattt atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg
    23221 gtgtgacaga agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa
    23281 atttttgtga aatttcagct tattttcgaa gaagctattt ctggaacaca
//
LOCUS       DACDCJ010000048        22361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDCJ010000048 DACDCJ010000000
VERSION     DACDCJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.048"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..144)
                     /locus_tag="IV038_001757"
     CDS             complement(<1..144)
                     /locus_tag="IV038_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8341963.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKT"
     gene            complement(275..859)
                     /locus_tag="IV038_001758"
     CDS             complement(275..859)
                     /locus_tag="IV038_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8341964.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1190..1423)
                     /locus_tag="IV038_001759"
     CDS             complement(1190..1423)
                     /locus_tag="IV038_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP8341965.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1438..2388)
                     /locus_tag="IV038_001760"
     CDS             complement(1438..2388)
                     /locus_tag="IV038_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8341966.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2385..3227)
                     /locus_tag="IV038_001761"
     CDS             complement(2385..3227)
                     /locus_tag="IV038_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8341967.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(3360..4085)
                     /locus_tag="IV038_001762"
     CDS             complement(3360..4085)
                     /locus_tag="IV038_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8341968.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(4546..4860)
                     /locus_tag="IV038_001763"
     CDS             complement(4546..4860)
                     /locus_tag="IV038_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8341969.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(4876..6471)
                     /locus_tag="IV038_001764"
     CDS             complement(4876..6471)
                     /locus_tag="IV038_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP8341970.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(6494..7384)
                     /gene="iolE"
                     /locus_tag="IV038_001765"
     CDS             complement(6494..7384)
                     /gene="iolE"
                     /locus_tag="IV038_001765"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP8341971.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(7401..8426)
                     /gene="iolG"
                     /locus_tag="IV038_001766"
     CDS             complement(7401..8426)
                     /gene="iolG"
                     /locus_tag="IV038_001766"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP8341972.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(8450..9457)
                     /locus_tag="IV038_001767"
     CDS             complement(8450..9457)
                     /locus_tag="IV038_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8341973.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(9570..10730)
                     /locus_tag="IV038_001768"
     CDS             complement(9570..10730)
                     /locus_tag="IV038_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP8341974.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(10806..12260)
                     /locus_tag="IV038_001769"
     CDS             complement(10806..12260)
                     /locus_tag="IV038_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP8341975.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            complement(12324..13148)
                     /gene="iolB"
                     /locus_tag="IV038_001770"
     CDS             complement(12324..13148)
                     /gene="iolB"
                     /locus_tag="IV038_001770"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP8341976.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(13187..15103)
                     /gene="iolD"
                     /locus_tag="IV038_001771"
     CDS             complement(13187..15103)
                     /gene="iolD"
                     /locus_tag="IV038_001771"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP8341977.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(15114..16148)
                     /gene="iolC"
                     /locus_tag="IV038_001772"
     CDS             complement(15114..16148)
                     /gene="iolC"
                     /locus_tag="IV038_001772"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP8341978.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(16305..17321)
                     /locus_tag="IV038_001773"
     CDS             complement(16305..17321)
                     /locus_tag="IV038_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8341979.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(17423..17620)
                     /locus_tag="IV038_001774"
     CDS             complement(17423..17620)
                     /locus_tag="IV038_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341980.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(17592..18911)
                     /locus_tag="IV038_001775"
     CDS             complement(17592..18911)
                     /locus_tag="IV038_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP8341981.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(19652..20632)
                     /locus_tag="IV038_001776"
     CDS             complement(19652..20632)
                     /locus_tag="IV038_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP8341982.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(20645..21523)
                     /locus_tag="IV038_001777"
     CDS             complement(20645..21523)
                     /locus_tag="IV038_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP8341983.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(21539..22339)
                     /locus_tag="IV038_001778"
     CDS             complement(21539..22339)
                     /locus_tag="IV038_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP8341984.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
BASE COUNT         6319 a         5054 c         3756 g         7232 t
ORIGIN      
        1 tgtctttatt ttacgaaaca tcaatactaa aactgtagca ataatgaaac accccacttg
       61 aaatatattt ccctttccta aaaaaataat gaccggaaaa aaaatattta aagtaatcaa
      121 ggttaacgga ttgatttttg acactctttc acctccaaaa aataagatcg cactgttatc
      181 ttaagctgat aaaaatgcac cgttattcag ataacagtgc gatcttatca aaaagcataa
      241 ctgagaacaa ttctcaatgc aagctttttt ttaattaact cagtaattca atccacccag
      301 gaacagaaaa ttgatacaat cttctggtta atagcatagt cttttctttt gaatagctat
      361 gcttcaatat atcatagaat gtagcgtaga atgagtaaat gtagaagtgg atttcttctt
      421 cattaaagac taaattttga ttgatcattc cgatagtctt taacttatct agacaacaga
      481 ctgtcttttt tgtcacaaat gatgtaattt tttcaacaat atcttgatat ttggttccag
      541 ctgattggaa taaaaccaaa tcgaatatac gtttattctc aaaaatataa ttaataaatg
      601 gatctaattc attgaagttc tctctccaac ttttctctat atctatattt tctaagttta
      661 agaggtattt ggtaaactct tcattataca tattaataag cgcattaata tgcggctgaa
      721 taatttcttc aaataacgct tccttagaag taaaatgttt ataaaaagca cccgtagtta
      781 cgcctgcggt caagcacagt tgccttatct tagtttgctg aaatcctact ttttcaaaat
      841 ggcttagtcc actttctatt aatttttggt gagttgtaat ataatctctg gtttctatgg
      901 gtacttcctc ctaataagat gaattaacta tattatctta cacaggactt gaagtttcaa
      961 tcgttaacgt ctattactga actcattcct tagagctaaa ctaatgagaa agagcaaaat
     1021 aactgataca aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt
     1081 ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc
     1141 ataatcactc caatagccat tttcttatcc tccctaaaaa taaagccttt tacttaaaac
     1201 cttttttgta atcgtcaata aattttttcc ctttatcagt taattgaaat cctatatcta
     1261 aatagtcctc atatctaagt ttagtcagca tctcacttac agatgctgga ctaacactta
     1321 gaacttttgc taaatcatta ttttttaatt tcttttttct ttttgataat tcctgaattg
     1381 ctagtaaata aattagtata gtagttgttt taatttgtac catacctacc ctccaaatta
     1441 tttcgaaagg ccttcagcaa ttttatccag gttccatttc atcatcgcat agtaactatc
     1501 accatcttgt cctttttctg caattgaatc agtaaaaatc gttgaataga taggaatatt
     1561 ggtatctttt gatactgttt tcattggtct atcgtccaca ctgctttcta cgaataagga
     1621 gggaactttt gttgtgcgta atttttcaac taagtgtttt atttgatctg gtgttccttc
     1681 ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca ttatacgctt tcgagaaata
     1741 tttaaagcat ccttcacttg taacaatcat ttttttatca ttcggaattg aagcaaattt
     1801 agatttagct tctttgtcta gagaatccaa tttttcaata tacttatcta gattttcttt
     1861 atagaatttt ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta
     1921 aataataccg ttttctaaat ttaaccaagc atggggatct tccttccctt tctcactctg
     1981 accctctaag taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc
     2041 tttgttcgca ttttttacta attttgtaaa ccaagcattt cctccagttt ccaagttaac
     2101 accgttataa aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc
     2161 atattcgtgg ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc
     2221 tatattttca gtaatatctg ctaaaatcga attagtaact actaccttta atttttcgtt
     2281 cgaagccact gtatctttgt ctttactatt tgttgaacat gccccgaata taaaaagagc
     2341 aatcaaaatc cccagacttc ccagtactgc tttccatttt ctcatcataa ctttctctcc
     2401 ttctttctta aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc
     2461 gctgttaaaa caatacttga tcctgctgct atgttaaaac tataaccaat gaacaaacct
     2521 agcaatgatg agcacgctcc gactgtcgcc gaaataaata tcatagtttt taaactgttt
     2581 gcatataaat atgctgtagc agctggtgta attaacattg caacaattaa tatagttccc
     2641 acactttgca tagctgttac tgctactaaa gttaatagaa tcattaatag ataatgataa
     2701 aaactgacat tcatccccat tgcttttgcc attaatggat caaaagaagt gattaataat
     2761 tctttaaaaa atagaacaac tgtaaccaac actaaaacag aaacgccaat tgtaatccac
     2821 ttatcaatat cttgtacagc taaaatattt ccaaataaga tatgaaataa atccgttgag
     2881 ctatttgcaa ctcctataag gataactcct aatgctaaaa aagaactgaa agtaatacca
     2941 attgctgtat ctccttttat tacactattg ctttttataa aagtaataat gatagaagct
     3001 aatagaccaa atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct
     3061 actcctggca aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata
     3121 ataaaacaac ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc
     3181 tgtaaaaagt gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccttg
     3241 tatattagag ttagggtttt aaccttccgg ttttgattgg ataaccctat gaaccatcgt
     3301 tattttaatt tggtattaac tcatttcttt tgagataata tatttattgg attgagaggc
     3361 taagccgttc ccccctctcc aataaatata gtatctccat aggctttttt taaattctct
     3421 tcattaaata cagattctac acttccgtga gcaattagat ttcgattcaa aagaataata
     3481 ttatcaaaat acttctttac cttgcttaaa tcatgatgca caattagcac tgtttttccc
     3541 tgatgtttca attcttttaa cgtatccata attattcgtt cactaactaa gtctatccct
     3601 acaaatggtt catctaaaaa tatgaaatcc gcattttgag caagacatct tgctaaaagt
     3661 acacgttgaa attgtcctcc agataattct cctatttgat gattagaata ttttcccata
     3721 tcgacctttt ccaaagcctt tgaaactctt tgccattctg catttttaat tctttgaaaa
     3781 actttcattc cggcatatgt tcccatagat acacattctt ttacttttat tggaaaagta
     3841 aaatcaatat cagctttttg ctctacatat gcaatcttca ccagtttttt attgatatct
     3901 tcattattaa tggagacctc tccctgatga gggataatat ttaacattgc tttaagcaat
     3961 gtagattttc cagcaccatt tggaccaata atacctgtta aagcaccttc ttgtattttt
     4021 aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa cattaagctt tttcacttct
     4081 aacataactt tctccttcta aatgataatg attatcacga tcatatggta tacaaaaatt
     4141 atattttagt caaccctaaa ttttataatt tagatttttt tctgttgttg aagattagga
     4201 tgtcaaaatt atttgaaact actcgtatag taaatagtaa gtaattttca aaacacgaag
     4261 aaaaacaaaa agaaaaaagg aaacgccgaa gcatttcctt tcagtgattt aatgagaaca
     4321 acacgatttg acttagagtc tcaaaaagtc gaataggtac actcatttaa gtgtctccat
     4381 tcgacttttt ggggatgagc tggctattga ggtccgctta ccctattgaa taaatacaag
     4441 attgggtatt tctaataagt ccgcccttca tattggggtg atcggggcga acatgccatc
     4501 ttttcatata ccacctcctc gacactcaat aatcaaagat tctcgttaat cttcttcgat
     4561 gcttgcaatg atgttttcaa tattcatata acgaaaatct tcttgtttga tataaacgat
     4621 ctgagcggta catttttcct ttttgatgat ttcttttact ttttcctcag aacgtgcgac
     4681 ctgcggcgtt aaaagtaaaa cgtcatagtc tttataattc agtttttctt caacgtttcg
     4741 gatcgttgca taatctatct catacgatag atttttggct ctgatcgttt caagcatttt
     4801 tgatacgagc agcgacgcac tgattccggt ctcgcaaata atcaatagat ccttatgcat
     4861 aataccctcc tgtttttagc ctgcaaagcc aattggtgaa aatgccacat aagttaaaat
     4921 aacaatgact accaatgcta tagcagtcac cttggtgtat ttccaaggct cgatatcgac
     4981 ctttgtatcg aatgagttca acacaaattc tccatcaggt ttcaatttgg aaaccaccat
     5041 caaaatgata atgtccagaa cgaataatgc cgtcaaaaca taaaggaaat ggatattttg
     5101 gaaaagcatt tttgatagtg catacaagac gatatgcacg gcaatcgtcg tttttgcccc
     5161 aaaagcagtt gctttttttg aataaaaggc aaagagaatg atcacgagca gcggcatgtt
     5221 gtatagtcca ttgaattctt gaacgaactg atacaatcca gccggtgcaa aagaaatgaa
     5281 tggcgctacg ataatggaga taatccccac aacgaccgtt actgttttac cagcacgagc
     5341 gatttgctta tcatttgctt tcttattgat aagcggttta taaaaatcca gtgaaaacaa
     5401 ggtcgatgtc gcgttcaaag ctcccgcaaa tgatgataag atcgcgccaa agataacagc
     5461 ggcgaaaata ccaaagatca ttttaggcaa tacttcactg actaaaaaag gatacgcgtt
     5521 atctggattt gacattaaac tatcgccaaa aatattgcga gcgacgatcc ctggaaagac
     5581 aaggaataac gcacctaaga ttttgaagac gccgactagg aaagcgccct tttgtgcctc
     5641 agctaaattc ttgccggaca atgctttttg gacgatcatt tgattggtac accagaaata
     5701 cagattgtta aacagcatcc ctgtaaatag tgtcggccat ggaacaacag ccgagtcgat
     5761 cgatcccaca gagttaagca gccatggcgt gtttaccaca acgtgttcga tcccgccaac
     5821 aaagctgccg tcaccaagct tcattaagcc taaaattggg accagcagac cacaagccag
     5881 cagaccgact ccataaatgg agtcactatt ggcacttaac gacaagccgc cgatcagcag
     5941 atagaggatg ccgacaatac caattgccat cgctacaatt atgatcgcga cgatcggctg
     6001 gacgcccagc agctcatcaa tatggaagat cttattgaac accagcgatc cagaataaag
     6061 aaccacgggt aagaaagaag tcatataggt cacaataaat aagatcgaaa taattcgttt
     6121 ggtaaccgta tcataacgaa tctcgatgaa atctgagatt gtattgatgc cgtatttaaa
     6181 atattttggc aagaatatca cagccaaagc tacgatcgca atcgtagcag ttacttccca
     6241 ggccattact tccatccctg catgataact ttgaccattt tgtccgacta tttgttcggt
     6301 ggacaggttc gtcatgatga tcgtgccggc gattggcaaa gcagttaagc tccgccctcc
     6361 catgaagaac ccttctgatc cagaagtatc gacacttctg gatctagaat aggcaaataa
     6421 ccatacacag atgacaatca ctaggaaact gattatagaa aaaagattca tcgtctagct
     6481 ccttttcaat agattaaaga ccggtgacct ctcgaatgta gcttctggct ttgactgcat
     6541 attcaaatgg attggcttta gccgggtcct gttctgcttc aactacgatc cagccttcat
     6601 agtcgctggc atggattgct gtccagattg gagtgaaatc aatcatccca tcaccaggaa
     6661 ccgtgaagac gcctttcttc acgccgttta agaagctgtc tttttccttt tctacttctt
     6721 cagccgtttc ttgacgaata tctttgaaat ggatgtgttt gatacgatct tgatgacggt
     6781 tgtaaaccgc gattggattt tcaccagaaa agaccaaatg tcccgtatcg aacaataatt
     6841 taactttgtc tggatcagtc atttccatta aacgatcgat ttcttccgtc gtttgaacgc
     6901 cggtacccat gtgatgatgg taaacgatct ccatatcctt ttcgtgggcc aaatccccta
     6961 atttttctaa tccttccgcc agttttttcc actcttcatc agtgaaaact ggtttatcat
     7021 caaagactgg cgctgtcata atcccttgga tactatgacc ctgctcagct actacgatga
     7081 ctttagcgcc catcgcatgt aagaaatccc gatgtgcttt gaaggcttcg gcagtttctt
     7141 caaaaggttt agttgtcaga aacgcactga accatgcact ggcaaccttt aagccgcgaa
     7201 tattcaaata ttgttccaac acctctggat cttttggata tttgttgcca atctctgttc
     7261 cggtgaaccc agcaagggcc atttcactga tacattgttc aaaggtattt tcactaccaa
     7321 gctcgggcat atcatcattg gtccacgcga ttggtgcgat ggctaattga atttgtttgg
     7381 tcatattctc acactcctgc ttattttttt agttgtacta aactcttcgt atcgaatgat
     7441 tcttgacaag ccaacgcgat cttagttgat tgcaatccat cttctgcatt aaccgctggt
     7501 tgacgttttt cttgaataca tgagacaaat tcttgggctt cacgaatgaa ggcttctctg
     7561 aatcgttctg ggaagttttg tgaacatgga cggatgatcc cttgatcgtt catgatcgtc
     7621 acaagatttt tctcaggcgc ttgcgctacc cgcagcattc ctttggttcc catgatctct
     7681 gtttcgacat gatagccatg ggctgcgttt ctgccggcaa ctagtaaagc tattgtttta
     7741 tcgctcaggc tcatcattgc ggctccagtt tcaagctcac cagcctgatc caattctgga
     7801 tacgccgcat ttttcccaat cgcccacact tgatcgactt cttggccggt aaaccagcga
     7861 accaaatcaa tatcatggat cgccatatct aagaacaagc cgccgctgtc gttattcatg
     7921 gcaaatttga caaagctttc caagccttcg ctcggatcga tcccataaca acgaatcagt
     7981 gtgatttcgc cgatttcgcc atcttctacc attttcttcg catactgata cgactcatca
     8041 tagcggcgca tgaagcctaa catgaagacc tgttcaggat gctcttcgat gactttttgg
     8101 gtctttttaa tatcttcaac ttcaaggcct aatggttttt cagtaaagac atgcttgcca
     8161 ttttccatcg ccaagcggac ttggtcacag tgaaagcctg atggtgaaac aataaagact
     8221 gcatcgatca cggaactttt gaccatttct tcatagctgg aaaaagtatg cttgacgcct
     8281 aatttcttct gtgcgaatgc taattctgtt tcaactaaac tgcacgcagc cgtaagctca
     8341 caacaactga cacctgttgc taaattttgt gcatgtactt ttcctaatct tcctaaaccg
     8401 acgatgccta ctctaatgtt gttcatgaaa tacctccgtt ttctgagaat tactcattgt
     8461 atagtgctgg tcgttcatcc atttgaatca ttacttttgt ttttgtttca cgagactcat
     8521 gacaagccat catggtgatc gctgctacat acccatccca agcggtagga ccgacaaccg
     8581 catcattttt gatcgaatcg atccacagct tgaattcata atcataggca tccaaaaagc
     8641 gggtctgcca atctggtgaa acaggtgtgt aatttttttc ttccgattta attcgtgtat
     8701 aggctgggtc cgtcattcct acttctgccg tttcgaaaac cacatcacat ttaatatcgt
     8761 agccaaagcg gcaattcaca aagatttcca aatcaatcgt tacaccggat tttgtttgaa
     8821 agatcagcag ctgcggatca tgcaaatcgg catgggagtt gctggttttt ttcggcaaga
     8881 tcatttgaac agattcataa tcttcattta gcaaccagcg aagcacatca atctcatgaa
     8941 tcgcggtgtt gctgatcgcc atcggtgttg tgtagttttc atctgcgcta ggatttctat
     9001 gggcacaatg catcattaat gcttctccgt attgcttcga ttcgattgct tctttcagtt
     9061 caatgtagcc gcggtcatag cggcgcatga agccaacctg aaccaatttc ttcccataag
     9121 caacttccgc atcaacgatc cggcggcagc ctgctgcatc catcgctaat ggcttttcgc
     9181 agaaaacata tttcccatgc ttgattgctt ctaacacata ctgctcatgg gtcgggtccc
     9241 aagaagtcac aacaacgaca tccacatctt cagaagcgat caatgcttct ccggtttcaa
     9301 agaatttagc atcgacacgt ttggcaacct ctttagcagc ttttacattg atatctgaga
     9361 ctgctgtcac ataagcccct tgtgttttct gattgatcct gtcgatatgc tcgatgccga
     9421 ttgcacctgt tccgataacg cctactttta ctctcaatta cttatccccc tgttctgttg
     9481 tttaagcaca catagtatgc gcttacaaaa ttattcctgt gtagctttta ttcttaaaag
     9541 gtagagccct ctctttgctc cgccttttac tacccataag cttgtacgta taattcctta
     9601 atttgactat gactaggcac ctttttatta ttttgcggac ttccgctggt caacgcatcc
     9661 gcggccattt tgtctaaggc tgccataaac gcttgctgat caataccata atctcgaatc
     9721 gttgggatcg acagctgctc acagagctct tggatttttt taatcagata atccgcagcc
     9781 tcttccagcg ataaatcttc ggctgccgga taaataaatc tggcgatcgt cgctaactca
     9841 gtctgaatag catcctttga gaattcaaaa aagaccggca ataacatcgc atttgatatc
     9901 ccatgaggaa tgtgaaaaag tgcccccacc ggtcgactca ttccatgaat caatgtgact
     9961 gacgcattcg aaaaggcaat ccccgcctcc gttgaagcta agctcatttg ttctctggct
    10021 tcagtgtcgt cgctattttc atatgcctga gaaagcgtgt tcaaaatttt cttcatcgct
    10081 tccagtgaaa atagttgcgt tacttcattg gctttgactg aaacataaga ttccaaagcg
    10141 tgacatagcg catcgactcc cgttgccgcg gttacttttg gtggtacagt tttcgtaaaa
    10201 tcagagtcaa cgattgagat ctgtggcatg aaaacgagat ccttcatcat cattttgact
    10261 gctgtttttg tattggtaat aactgccaca tcggttactt ccgaaccagt tccagcagtt
    10321 gttgacactg caatcagcgg aatgcccgct tgcagctgat caaaatttct tgcatgaatc
    10381 gcttcgagac ttaattgatt agcagccaaa acggctacca ccttggctgt atccagacag
    10441 ctgccgccac caaaagcaat gatatgatca cattgatgct tctgaaaaag cttcagcgca
    10501 tcataaacat tttcttgcgt cggttcggct tgaatgtctg tatactccgc taattgatca
    10561 tctgtttttc ccaacagctc cttgatcttt tgaagatgcc cggtctgttc cataaccgta
    10621 tcactaatga ttaacggctt gttgcctagt tgttctgatt cttccataag aaattggatt
    10681 gttccgccgc caattcttaa attggcaggc attttcagtt gaatattcac agaaaaaacc
    10741 tctctctggc ttatagccta taacagaatc ctcgaagctc tttaacagag cttcgaagca
    10801 aatctttaaa tctcactttg cgcataacgt gctgtaacaa ctttccgatg ggtataaaac
    10861 tcaacactgt ctttgccatt ggcatgcaaa tcaccaaaga aagagtgctt ccagcctgaa
    10921 aaggcaaacc aagccatcgg tgcaggcacc ccaagattga cccccagcat cccagcatca
    10981 acattttctc tgaagtagcg aatcgctgat gcgttgttgg tatacagaca cgaaccattc
    11041 gcaaattcag attcattcgt cagctcaacg gctgccatca aatcttttac ccgcacgatt
    11101 gatgcgaccg gggcaaaaat ctcatcctgc cagatcttca tatccatcgt gacattatca
    11161 aaaatcgttg gtccaacaaa atagcctttt tctggaatgt tttcgcgacc atctagtacc
    11221 agtttcgctc cttcatcgac ccctttttgg atatagtcaa aggtccgctg ctgtgcatct
    11281 tttcgaatca ctggtcctaa aaagacccct tcgtccaagc cattgccgat cttgatcgct
    11341 tttgccgcag ccttaaattt ttcaacaaac tgatccgcga tttcctcttc caaaacaatg
    11401 acggaggctg ccatgcagcg ttcaccagca gaaccaaaag ctccgccaat aaggtccttg
    11461 actgcccgat caatattggc atctttcagc acaacaacat gatttttcgc tccagtcaac
    11521 gcttgtaccc gttttagatt ttttgttcct tcacggtaaa cgtattcacc gacatttttt
    11581 gatcctacaa agctgatgcc ttttaccagt ggatcttgca gcaatgtgtt gacaacatcg
    11641 tgggcaccat agacaacatt aaaaacaccg ctaggaaagc ctgctttttc aaccagctca
    11701 acaattttct ccatcaacaa tggtgttttt tctgaaggct tcagcaccac cgtatttcct
    11761 gccgccagag ccatcgggaa gatccagaag ggcaccatca ttggaaaatt aaacggtgtg
    11821 atccctccga ttactccgat tgggtaacga taattcgtga tctcaatatc agtcgccgct
    11881 gttgataaag aatctcccat cattaaattg gtgattcccg ctgcgtgctc cacattttcg
    11941 attcctcgtt gaacttctcc cagtgactcg gacaatgatt tcccattttc cagcgtgatc
    12001 aaacgcgcca attcactttt atgatctgtt aaaagctgat gcagcttgaa cagataacgt
    12061 ccgcgaacag gaacaggaac ttttttccaa gtttgaaacg cagcatcggc agctttaact
    12121 gcttcggctg tctctgcttt gctagacaac ggtacattcg ccagcgcttc ttctgtagcc
    12181 ggattcatta cttcttcaaa ctctgttgag cgactagcga cccaacgacc atcaatataa
    12241 tttcttaaaa atcttgtcat ttattttcct cctaaaaacc taactaaacc taactaaact
    12301 ttatgtatct aacagcttaa taactacttg cgctgcaaga tccattcgtg tttcttttca
    12361 ttgtggaatt tccaaattcg ctcaggtccc gccatcacat ttaaatagta ggaagtatac
    12421 ccgtcaggta ccccaactgg atgatagccc ttaggaactt cgacgacatt ttgatgctct
    12481 accgtcatcg tttcatctag tgaacgatct tccgtgtaga cccgctggaa aacaaatccc
    12541 tgctctggat caagctcatg gtaataagtt tcctctaaaa aagactcatg tggcagattc
    12601 tcttgatcgt gcttatgagg aggatagctg gaaaaattac cgccctcggt ataaacttcg
    12661 acgaccagca ggctgctggc agtatcgaca ttatccggta aaatgttgtg aacaagtcgt
    12721 ttattctgat agagtccacg ttcttcgacg gtattatcct ccgcgcgaat cacacttatc
    12781 ggcaaagctt ttttcgctgg tgaatagcaa agcgccaatc ggacattgtc tgtagttgcc
    12841 acaagttcaa acgaatgctc cgccggaaga taaatactgt cggttggaat tttttcaaaa
    12901 acagattctc gtgttcctaa atcttcatac caagtgtctg ctgctttaac cttaattttt
    12961 ccagtcaaaa tgacgataca gacctcctgt tgactgccgg tttccttgta agaagctccc
    13021 ttttccagct ccaaaatctg aaattcgata taactcaaag cagaattaga ctgccgaata
    13081 ttttggattt gttttactcc aggtgccagt tctttgttta atggttgatg cttcaatgtc
    13141 ttttccaatc aatccactcc ttattttcaa tcacaaaaag caatatttaa tactttctga
    13201 tagtggcaag tttcgcttgt ttggctttgt aagcctcctg catggctgct ttacccgata
    13261 cctctgagat accgacgttc caccagctgc cgtcgtagcc ttctgtcatg gttttaggaa
    13321 gtacctttat ctcaatcaac gtcgatcgcg tttgcttctt cgcatcctcg accgctgctt
    13381 gtaattctgc aatcgagctt acacgatacg tttttgcacc atagccttca gcaactttgg
    13441 cgtaatcgat gttcatgatc tcgtccttgg tatttctgaa ttcggttcct tggctagctc
    13501 cgccattatc catctgtaaa ttattgatac agccgaaacc agaattatcg aaaagtacaa
    13561 tgttgatttt tttatcatat tgcaaactag aaaccagctc gctatgcaac atcaagaaac
    13621 tgccatcgcc tactaaggca tagacctctt gttctggtcg agccattctt acgcccatcg
    13681 caccagcgat ttcatagccc atacaagaat aaccgtattc caaatgatag gtattctcct
    13741 tcacagggtt ccataagcgc tgcatatccc ccggcaatga accggctgaa ccgatcacga
    13801 tcgcatcctc atcaacaaaa tcatttaaat gaatgaagac ctctgtttgc gtcaaattcg
    13861 tagccaacgc atcagcatat tcattcaaga tttcttgaga aaaatgatct ttgatttctg
    13921 gcgtaaaatc tttacgctta aaatgcgcgg cttttaaccg atttcgctca ctgatccatt
    13981 cttccttcag ttcatcgatc ttctcgccat aggcggtttg gtaaccttcg atcattgggg
    14041 caagctgctc caaggtcgct ttcgcatcac caaccacttg aaaggcttcc agcttagcgg
    14101 cctgttgtct gcttacgtta atattcatca ttttcgcttg ttcaaaatta aaaatacttt
    14161 tagaagctga ggtaaaatca gtgtagcgtg tgccgatccc tatgatcaga tccgcttgct
    14221 gcacagcttt attagcagcc gaagttccca aaatccccat tccgccgaga ttgtttttaa
    14281 aatcccacga aacagtcgat ttacctgcgt gcgtttcaac tagcggaatc tgacaatctc
    14341 ttgagatttg ctcaagaatc tcaccagcct ccgaatattt gcagccgcct cctacgatga
    14401 tgacaggtcg tttactggcc tttattcgtt gactcgcttc agcaagctca cgagcggtcg
    14461 gaatcctgcg atcaatataa tgtactcttt ttttgaaaaa ttcctccgga taggcatagg
    14521 cttctgcctc cacgtcctgc ggcaaacaaa tcgttgccgg acccatcgtt gccgggttcg
    14581 tcatgacttc aaaggcccgc aataacgcgg acatcaactg ctctggtcga ttgatgcggt
    14641 cccagtagcg agaaaccgct tgaaatgcat catttgtcga aaaggctaca ctgcttgcat
    14701 gttccagctg ctgcaagacc ggatcaggct gacgtgtagc aaacgtatcc gccggtaaaa
    14761 aaagtacggg gatattattt gcgaatgccg tacccgctgc tgttaacaga ttggccgcgc
    14821 ccggtccaac agatgttgat acggcaaaaa ttttcttgcg taaattctgt ctggcaaagg
    14881 cagtagccgc atgggccatt ccctgctcat tttttccgct ataacttttc aaatgtccgg
    14941 gctctaattc cagcgcctga ccgatcccca aaacatttcc gtgaccatag acatgaaaga
    15001 taccttcaac ataaggcgtt tctttgccgt cgatgctgat gtattgttga ttcaaaaatt
    15061 ttagtaatgc ttgtgctgtt gttaaacaaa ccgtcttttc catttttttc accttaaatc
    15121 cgagcgatca tttcgccgta tatttctttc tccaatgtaa taaaatcttt tacctcctga
    15181 gtcgtcggta aagaatctga acagttattg cttttgacca tcattgatgc ttccgctgag
    15241 ccaaactcta aacaatcgat gatctcccag ccttcgaata aaccgtataa gaaacccgag
    15301 ccgtaaccat ctccgccgcc aaagcctttg cgtgcttcca ccggaaacgg cttgattgaa
    15361 aactgctgct gatcggccgt ataagcagtc gatcctttca ttccatgttt gattacgaca
    15421 atctcagcac cacaactgtg ccaaaaagca gcgctttcat tatctgacat gttaggctgg
    15481 atcaacttct cagttagatc gaactcttct cttgaaccca tgataatgtc tgattcctta
    15541 gcaatcatcg aatagtaaat tgaaatatca tcgttgttct tccagttata agcacggtaa
    15601 tcaatatcaa aaataatttt gacattattc cttttcgcca gcatcactgc cttgattacg
    15661 gcttctctgg acgggctctc tgctagtgca gttcctgaga ttaatagtgc ttttgaccgt
    15721 ttcaagtagt cctcatcaat atcctcaacg ctaagctgca ggtctgcggc ttgatttcga
    15781 tacatcaaaa tattactttc ctttgatgag agcatttcgg taaaagtcag ccccagcttt
    15841 tcaccatgct gcgctttgct gatctgagac gtatcgatgc cttgttcatt gaaataatcg
    15901 atcacatatt cgccaaactg atcgtccgag actgttccga taaaaccagc tttcatgcca
    15961 tgtttagtaa cacccacagc aatattggca ggagatccgc ccacaaattt ttcaaacata
    16021 tggacctttt tcaatggttt aaactcttct ttgacttgat cattgtaggc aggattaaaa
    16081 tcaatagcga ctcttcctaa aagcagcaag tcaaattctt ttgattggtc aaactttata
    16141 taactcaata tatccacctc tttatccgtt cttctatttc tgaggcaagt atagcacgcc
    16201 aaaacaaagc gtgcaatatt tattttagaa cgcaatattg cgtgctgttt gaaaatgacg
    16261 gcaaaaaaaa cgtgaatcgc cttacatcac gattcacgcc tagattaaat attgtatttt
    16321 gtcttaataa ttatatcagt aaatagcgca tcttttgtaa ccgttttatt attttgcagc
    16381 tgattcgcta acatcagtaa tgaacgatag ccttgaacaa atccatcttg atcgattaga
    16441 aaatcaacct gatcgttttt cagcatctcg atattgttct tagtcaaatc ataaatgatt
    16501 acaaagggcc gcttgctttc atcgattttt tccaacgctc gggcaattcc cgcttgtccg
    16561 ccagaaaaga cgactacgcc atctaagttt gaataattct ccaaggtgtt gcttaaaatc
    16621 ttttcaacct cagcactcga atcaaagctg ctctgaacac ccgtcagctc cagttcagga
    16681 aacgactctt tcaattcttc aacaaagcct gccacccgta agctgttgac gctatttcca
    16741 aagtagccgg taatcgctag aaccttgccc tttccttttg tcagcatacc taacaatccc
    16801 gcagccgttt ttccgctttt gaagttgtcc atcccaacaa aacactgtcg attcgtacca
    16861 agaatatccg aattaaaggt aatgatgagg atgccttgat cggtcaattg attaattttt
    16921 tctctgatac catcactatc tactggcatg atcgcgatcc cttttactcc aagctccacc
    16981 aatcgatcca acgcctgcag ctgggccgct tcatcgaccc cgtctacctc ttctaaaaaa
    17041 caatcaatgt ttcgcttttt taaatccccg ataatagatt ccagacctga tcggatcgga
    17101 atcataaaag aagatttcgc caattgagtg acaatcccca gcttgagcgt tgtctctttt
    17161 atgaccttct ttttctttgg aacatagttc atttccttgg cgattgctct gattctttct
    17221 gcaacctcag gattgatcct tcctcgatcg ttcaatgccc gatccactgt cccccttgac
    17281 actcctgctt tctcagctat ttctttaatt gtacccgcca tcttgcctct ccttttttcc
    17341 tattgtgttt attttatcac aaaacttaag aaaaccaccg gagaatgaat tctccggtgg
    17401 actttttgta tgattaacct tcttattgaa catcactgat cttaaactct attccaaggg
    17461 cgtttctata atattctgga tacatctcct tcccacattt ttcacaggta aattggggag
    17521 gattgttcgg atcatttgca atatctgcat catctaaaaa ttttactaca tccattggga
    17581 ttgattcttt ttcaccacaa cttagacata taaatttcac gttggcgttc ccaatcccag
    17641 cgaattgctt taaaagtttt ttctttttct tcttcatact gttgcctgaa ttctttttgg
    17701 tcaatttttc gattctcctc tcgcataaac cgctttgcat ggttatcttt cgcatattta
    17761 ataactaacc gtccattttt gcgcacactc atcccaacga attcatagta ctcaccgcaa
    17821 caagaacatt ctaagggatt ttcaccaaat tcttcggtta ttctttcttc ccaggatttt
    17881 ggtcttaagg cctcattcag attgataaac agctttttta ctcgtttctg atactccttc
    17941 atgacctttt tcattaatgt tttgattcta cgactataga ttccatagcg acgaatcgtt
    18001 ttgaaattct tattttgaat atggcgaatc agtgcagcta tgaattcgtc tatactcatg
    18061 gtcatcgtct ctttagtatt cgttcgtttg tccatgtagt tgaacatgac agtgtcgcca
    18121 tcatacatta agattcggct taatgcgatc gggccacgct tcatataacg actgatgtat
    18181 tggagtagtg actttagatt tgtcctgctc cttttaggag catttacgta aaacccttct
    18241 ccattgttct tgtacgcagc ttgtaatctt gattgaactc tttgcttgtc ccactcacct
    18301 aacgttcgtc taattagttt caatacagca tttttccagt attttcttaa catagaaaaa
    18361 ggtaaataat cgtattcttc ccaacttcca tctcttgtta agccacccat agtaacaacc
    18421 atatgaacat gcggattaaa ttctagtttt gatccaaagg tgtggattgt agctactata
    18481 cctgccttca cctttctttt ctcaaaatag gcaagaataa tttttgcggc ttcatccatt
    18541 agtcctttca gtaaggaatc tcgatagttt tccatcataa agatttcccg taacccctca
    18601 tcgatcgtaa agattacgtg ccgatgtgtc actttaaaca tatcattcgc cacaaccgct
    18661 gaccaccttt ggctttcacc tgttgcacac gttggacaga actttccacg acatttcaag
    18721 ggaacaaact ttgtttgatg acatccctcg cagacaaaca gtcggtatcc tttttgaata
    18781 tctccacaat atctaaactt gtttacttcc cgtttgacga ctagccgaat ctttcgcccg
    18841 tatttctcaa caaagttatc ccaattatgg tgttcgtcaa aaaagattgc ttcgagaata
    18901 ttcgctttca tagtaatatt ttacttgttc aaaaacaaaa tgaaaagggc taaaagactg
    18961 gtagtgcagc ctttttagcc cttaactttt tatactttct ttactgtcta aaaaatatgc
    19021 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcgccaacac
    19081 aatttgacga agagcctttt ctttcaggaa gacttcggta atttttagga gttctaccta
    19141 ttcttttaac cgtttattct ctttttctaa gcgtgtaatt tcatttctgc gaatggattt
    19201 catgacttcc tctaccgaga aaagtacgtt atccatattt ttcaacttct tgaactcatc
    19261 aatacacgct attagagcga gcttctagct atagactaac ttctttaaca gaaaaccctt
    19321 cttccgttac cagttttaca gcggtaattt taaatatttc atcaaatgaa cgtcttggca
    19381 taatccagat cttcgctttc tcttaatttg gtgtcaattt tagtataacc cgtccaactt
    19441 attttttaac taagtgcccg gtttttcttg acaactccac ccaaaagtta gattttttgg
    19501 tctaagtttt agggtcgctt cattactaga attcaacaag agatgttttt tattttattt
    19561 aaattatggg gtcagtttcc atttgattac aaacttcttt gttttctttt tttaaacgca
    19621 ataataaagg ttttaatttc agccttttta attagaaaat caattttccc aaattatttt
    19681 ctttagcttt cttaacgatt tctgtcgcaa ctaccacatc taatacagcc gaaccaactg
    19741 ttttgaagat cgtgatttgt tctccacttg tccgaccgat caattcttca ttgatcagtt
    19801 ggcctagttc cccatgatag ctgtctcttt gaatataccc atcttgcagc ggtgaaatga
    19861 agtccccagc ttcttttaga acaccgtcca tggtatcaaa aataacaaca tctgcggctt
    19921 tgattatttc tcgtggaatt tcacacattt ccggtgtgta agcaccgact ccattgatgt
    19981 gcgcaccttt tttgacccac tcagcagaga atgtcgcacg ttttgaagtg gtcacacttg
    20041 taatgatgtc tgctccttca acgcattctt gactggtttt tgttgggcgc atcgttacag
    20101 aaaattgttg catcattttt tcagcaaatt ggctggcacg ctcaaaatca atatcaaaaa
    20161 tccgaacttc ttctaattta cgaacggtca acattgcttc taattgtgac tgtgcttgtc
    20221 cgccggttcc aatcagcgcg ccaattttag catcttctct tgcaagaagt tctgttgctg
    20281 ctccttgaac agcgcctgtt cttaattgtg tcaaataagt accatccaaa caagcggata
    20341 ccatacccgt ttctggatcc aatacgatca tcgttgctgg tacacttggt aagtttttct
    20401 tgatgttttc aggatacacg gaaacgattt tgacacctag cgcgccttcg ccaccttcta
    20461 cataagcagg catatataag ctttgtccgt tgctttttgg cacatcgata ttggtgcgaa
    20521 gaggcacact tgcctttcct gctgaataca atgacagtgc ttttttgtcc gcatcgatgg
    20581 cttctctcat tgaaaaactt ttgataatat cttctttttt aagaaataac atttgaatcg
    20641 ctccttaatt agctgctgta acttttttta attgactgcc caacatcgta tatgcactgt
    20701 atagtaagac caaaatcacg atccatttca ggatataggt atctaaagat ttgactagga
    20761 atacagcgac caataccccc aatacaccga aggttgaagt aaaaagtgtg atttttctgc
    20821 tgtattcatt aaatttaatg aattgaacac tgccgatcgg tactgagaag gtacaagccc
    20881 ccatcataat aggaaaggct gcaactggat tcaagcctag caagtagaca gttaccatcg
    20941 ttaacgcata agaaccaatg ccgatattgt tcaatgcacc atagacaaag agtaaaaatc
    21001 ctgccaagat taattttccg ccatacaatt cactggctgt tccgtttgat ggaatccagt
    21061 tcatttgtcc tgcaaaaatc aacaatgcag caatgaccaa tcctatgccg acaaacagtt
    21121 tgatcgtttt ctctggtaac ttcacgacaa atcttggtcc taaatatgca ccgataattt
    21181 ggcagataat acaaacagct agtgtcttga tccctacgtt gatggcagtg atgtaagaca
    21241 atgccatgac ggctaccggt actacacatt gggtattcaa cgttccgggt agttttttca
    21301 tgctgaccca tttcaacttt ggataaagga cggttccgat agcaaagtcg gaaacgccaa
    21361 atgttgaaag aaagaagatg ataaatgacg tgaagggcaa cagacgcata tcccctggtt
    21421 cttctttgaa attttgtttg tttttgaaaa ggtctcttag aaagtaaata ataaaatata
    21481 gattgattgc gacaattagt gtcagcaaaa tattccgaat cataaaaaat tcctcctact
    21541 agtaaatctt tctgattccc tcaaagccct caccatattt tgctttaagt tcttcaggag
    21601 attctgcaat cacttttagt gtatatgcat catgtttgac atattctttt gctagattga
    21661 ccatggcgtg gttttttgtc caagtgacat cggttgattg cagttcttcg ttaatggtac
    21721 caaacacgac ttcttgctca tcggcgacga tgttgatcca tcttgctcca aagtcagcaa
    21781 gtttgtctgt ttcaaaacca tgaatataat acttttctaa gggaagatca tattcatggg
    21841 tagaactgaa taaaatcagt acaaacttat caactctttt ttcagcttgc tgcaatgcct
    21901 ttagtagtga atccgtgaaa ttctcatgcc aaacttgaat gagcaaactt tcttttgcgt
    21961 ttttcaccaa atgctctact ttatccagaa catactcgat accatcaact ttccacagca
    22021 tttcttcttc atctttttct ttgatcatcc ctagattctt ggtaagaaaa gaaagatcat
    22081 ttttaacact tttttctaat ttctctaaaa actcatttgc cgagatggca tgataaagtt
    22141 taggttctga tttattaacg acaatcaaat tcttttttaa tagtttttcc aaaatattat
    22201 atactttcga gcgtggcaca cctgagattt tacttacttc atatcctgta agattacctt
    22261 tttctaatag tgtggtatac actcttgtct ccatctctga aaaatcatat tttttcatga
    22321 tttgaataat cgtttccatt gattaatcct cactttattt t
//
LOCUS       DACDCJ010000049        20696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDCJ010000049 DACDCJ010000000
VERSION     DACDCJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.049"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(640..948)
                     /locus_tag="IV038_001779"
     CDS             complement(640..948)
                     /locus_tag="IV038_001779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341985.1"
                     /translation="MKLEIKEVVCDWGIYVDGETYPFMIFNSKANAQEIMRIMELDNK
                     HERFDSVKKMENECEAVEYQKTCANNKYILGAMGLGKSASIKQVYEESQENCVYENSN
                     "
     gene            complement(1392..2003)
                     /locus_tag="IV038_001780"
     CDS             complement(1392..2003)
                     /locus_tag="IV038_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8341986.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            complement(2043..2825)
                     /locus_tag="IV038_001781"
     CDS             complement(2043..2825)
                     /locus_tag="IV038_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8341987.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            complement(2839..4221)
                     /locus_tag="IV038_001782"
     CDS             complement(2839..4221)
                     /locus_tag="IV038_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8341988.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYELYIKDFPEIPYKVFKD
                     IQEKYSDTFSLDKDYELLNPIIKFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTT
                     LTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNVELKNLNIGNNIKNIRKSKN
                     MTQTDFAELMGLSRSYIGDLENNRRNPSIKTLEAIARALDVPIVALINDSSTDNIVEP
                     YESLTAENILLKQENEELKYEVESLRKRLYEIQQVVNQGAEKMIPNLKDVSKRIKYIR
                     KKLGMTMEQFGSHLSNSPKSTIATWESGRNIPSQKKLQLIAIVGETTTDWIKWGTLEE
                     YITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDKDYELLDSIIKNIFTKYY
                     SKEFEVTQIP"
     gene            complement(4270..5166)
                     /locus_tag="IV038_001783"
     CDS             complement(4270..5166)
                     /locus_tag="IV038_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8341989.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            complement(5361..5903)
                     /locus_tag="IV038_001784"
     CDS             complement(5361..5903)
                     /locus_tag="IV038_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8341990.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYETKFCKRGD
                     CMKPNPVETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERGFSLPNKERLIKIA
                     ELSNKTIDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKDIQEKYSDTFSLDK
                     DYELLDSIIKNIFTKYYSNG"
     gene            6074..6262
                     /locus_tag="IV038_001785"
     CDS             6074..6262
                     /locus_tag="IV038_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP8341991.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            6308..6685
                     /locus_tag="IV038_001786"
     CDS             6308..6685
                     /locus_tag="IV038_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP8341992.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(7311..8483)
                     /locus_tag="IV038_001787"
     CDS             complement(7311..8483)
                     /locus_tag="IV038_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341993.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIMFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(8727..9695)
                     /locus_tag="IV038_001788"
     CDS             complement(8727..9695)
                     /locus_tag="IV038_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8341994.1"
                     /translation="MILENDKPYYVVIGGVNGAGKSTIYQIQNSKRKELDTDRINADE
                     ILRRFGGNWNKQSDINKSMREVLREIEGHFKEGKSFHHETTFGGNPYPDKKRLLNAKK
                     LGYESFLIYIGLDSPERAIERVKSRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            complement(9692..9889)
                     /locus_tag="IV038_001789"
     CDS             complement(9692..9889)
                     /locus_tag="IV038_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341995.1"
                     /translation="MRDMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLV
                     LSSYVTRVDQKKVINQKDFKI"
     gene            complement(9900..10217)
                     /locus_tag="IV038_001790"
     CDS             complement(9900..10217)
                     /locus_tag="IV038_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341996.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRLIEVNNLGIIEDYLAKLDEAVKQSIEKNGEDHVLTVVLKDLKDRLEAEAENF
                     NKD"
     gene            complement(10254..10472)
                     /locus_tag="IV038_001791"
     CDS             complement(10254..10472)
                     /locus_tag="IV038_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341997.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKANGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            complement(10581..10772)
                     /locus_tag="IV038_001792"
     CDS             complement(10581..10772)
                     /locus_tag="IV038_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341998.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            complement(10934..11290)
                     /locus_tag="IV038_001793"
     CDS             complement(10934..11290)
                     /locus_tag="IV038_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8341999.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            complement(11315..12319)
                     /locus_tag="IV038_001794"
     CDS             complement(11315..12319)
                     /locus_tag="IV038_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP8342000.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            complement(12356..12616)
                     /locus_tag="IV038_001795"
     CDS             complement(12356..12616)
                     /locus_tag="IV038_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342001.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            complement(12625..13698)
                     /locus_tag="IV038_001796"
     CDS             complement(12625..13698)
                     /locus_tag="IV038_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342002.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            complement(13706..15532)
                     /locus_tag="IV038_001797"
     CDS             complement(13706..15532)
                     /locus_tag="IV038_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8342003.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATSEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKNDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            complement(15687..16553)
                     /locus_tag="IV038_001798"
     CDS             complement(15687..16553)
                     /locus_tag="IV038_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342004.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLIVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            complement(16728..16991)
                     /locus_tag="IV038_001799"
     CDS             complement(16728..16991)
                     /locus_tag="IV038_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342005.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            complement(16993..17322)
                     /locus_tag="IV038_001800"
     CDS             complement(16993..17322)
                     /locus_tag="IV038_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342006.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            complement(17344..18879)
                     /locus_tag="IV038_001801"
     CDS             complement(17344..18879)
                     /locus_tag="IV038_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342007.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            complement(18946..19413)
                     /locus_tag="IV038_001802"
     CDS             complement(18946..19413)
                     /locus_tag="IV038_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342008.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            complement(19410..19706)
                     /locus_tag="IV038_001803"
     CDS             complement(19410..19706)
                     /locus_tag="IV038_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342009.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            complement(20057..20287)
                     /locus_tag="IV038_001804"
     CDS             complement(20057..20287)
                     /locus_tag="IV038_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342010.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            complement(20323..>20696)
                     /locus_tag="IV038_001805"
     CDS             complement(20323..>20696)
                     /locus_tag="IV038_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342011.1"
                     /translation="EIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKDE
                     KKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEESE
                     NKIKQFEEKIKQLEKSASRFE"
BASE COUNT         6017 a         3903 c         2490 g         8286 t
ORIGIN      
        1 atctatatct ataggatcac ttaatttact gattaaatca gcaacaccat tttctaaaat
       61 gtaatctaaa ttattattta atactctcga cttaacgtac tctgatacgt tagaattttt
      121 tagtgcttta taaatagctg tcattcttta ttctccttcg tagccttttg atctacgaat
      181 ttaactaccc tttttctata tacaaccctc ctcattcaaa attagatagt caagacgctt
      241 aacaagacta catttctatt ttgtatgatt taccgctata attcaacctc aattaaaatt
      301 tatttcgtgc ctaacgattt aaatattcag ttactttctt tattaaaaac agcaaactat
      361 aaatgataat aaaatcaaac actattaaaa tcatacttga tattaaagct ttatttgtta
      421 atgttgtaat cccaatagta attactatta agtttgtgat tataattcta ccaatattac
      481 tattttcatt cttatgaatt agtaaatcat agacatcaaa tacgataata caaactatta
      541 agaacaatat aaatattacc ttattgttat atacaaattc agttgatttt cctattaagt
      601 ataaaactaa ataaattagc tcccacatat tctcactctt taattcgaat tttcatatac
      661 gcaattctcc tgtgattctt cgtagacctg tttaatagaa gctgatttac ctaatcccat
      721 tgcacctaaa atatacttat tattggcgca tgttttttgg tattccactg cttcacattc
      781 attctccatt ttttttactg aatcaaatct ttcgtgctta ttatccaact ccatgatacg
      841 cattatctct tgtgcatttg ctttactatt aaaaatcata aatggatatg tttctccgtc
      901 tacataaatt ccccaatcgc agacaacttc tttgatctct aatttcataa aaaacacctt
      961 cctgtagata atcaattatc actcatgctt atcaattgat tcttgaatgg tattgcaagg
     1021 aaatgtattg aatctgtttt cgtcatattc taacgttgta tactgtggcg taagagaact
     1081 attatcttca acacttattg aatcgaatat gcaacctaaa attctgacca gtcataattt
     1141 ttttcttctg tactttctgc tgtaggattt ccaaaaaatt tcagtcgaaa ttctttttca
     1201 aaatcattaa cccaattaac ttgttgattc actctcgcat agcctctttc ttttctattt
     1261 ctgtcgttac ttgactgcca caaaattaat tcttttgttt agaagcttct ttgtataatt
     1321 gtacacctta aaagtttgaa acaggcttag aatccttctc tcgtaacatt gatttctcaa
     1381 agaacttaaa atcaatcaga ttcacttatt cttttcttta ttctatagac taaatttcta
     1441 tttgttcctg ttttttctgc aattcgctta atcggaacac cttctccaag cattcttact
     1501 atttcattat agataaacct cttttgtgga ttctttgcag tagaagaata ttcgtagggt
     1561 tttcctttat agactccttt cttcttcgca attgctattc cttgtttttg ttttctctta
     1621 atttcctccc tctcattttg agccgtccaa gaaaaaatgt taattactaa gcttctaaca
     1681 agtttttcta agtttggatc accaaatttt gtatctaaaa ttggtaaatt caaaaccatt
     1741 aagcccatac catttttatc tataaaagat actgtttcta gtatttcatc agaatttctt
     1801 cctaagcgtt caagcgactc aaaaacaagc gtatcccctt ctctgacaaa tgacaacatt
     1861 ttattgaact ctggccgttc tactgtgtta cgtccagatt ttttctcagc aaatactttt
     1921 tcgacaccaa tttttctaaa ttcttcaagc tgtctttcca aattttgatc atctgaactg
     1981 actcgtgcat agccaactaa catatttaac acctacaaat aattttactc tagtttaatt
     2041 aactaggaat tttttcgtga gttcagcaga ctctctatac catcaaataa acattcatag
     2101 tattttattt tttcttttag ctttgaattt tcttctttga gttcatttat caatttaact
     2161 tgggattttt cagaaataaa gccttcatca aaaagcaaat cttctacact acaattcgca
     2221 atgtctgcaa ttttttttag tttggctttg ttcggtaagt taccaccatg ttcccatgtt
     2281 gacacagtgc ttctaggaga attagaaacc tcgtacccaa attcctgcat agtaagtcct
     2341 aatttttttc taatagaacg aatttttctc cctacctttt tagggtctgg tttaacttct
     2401 atgtctcgat aatcttcaaa atcttttgag taatattttg taaatatatt ttttattata
     2461 ggattcaata actcatagtc tttatttagc gaaaacgtat tactatatct ttcttgaatg
     2521 tctttaaaga ctttgtgagg tatttctgga aagtccttaa tatatagctc ataccctata
     2581 tcgataaggt atgaagttat atattcttct aaagttcccc atttaatcca gtcaattgtt
     2641 gaattagtga tagttgataa taatttcagc ttttgtggat ttggtaaatt ggtcccacgt
     2701 tcccagttgg atatggtcgc acgtggtgaa ttatctattt tccttccaag ttcttccata
     2761 gtaaaaccac atgaaaggcg taattgtttg attcgttttc ctgcttctga aggatttggc
     2821 ttcatacaat cacctctctt atggaatttg ggttacttca aattcttttg agtaatattt
     2881 tgtaaatata ttttttatta tagaatccaa taattcatag tctttatcta acgaaaacgt
     2941 atcactatat ttttcttgaa tgtctttaaa gactttgtga ggtatctctg gaaagtcttt
     3001 aatatatagc tcatatccta taccgataag gtatgaagtt atatactctt ctaaagttcc
     3061 ccatttaatc cagtcagtgg tggtttctcc tactatagca atcagctgta actttttctg
     3121 agaaggtata tttctaccac tttcccaagt agcaattgta cttttaggtg agttgcttaa
     3181 atgagaacca aattgctcca tagtcatacc taattttttt ctgatatatt tgattctttt
     3241 acttacgtct tttagattgg gaatcatttt ttcagctcct tgatttacta cttgttggat
     3301 ttcatacaat ctttttcgta agctttctac ctcatatttt aattcctcat tttcttgttt
     3361 aagaaggata ttttcggctg tcaaactttc atacggctct actatattat cagtagaaga
     3421 atcgttgatt aatgctacta tcggaacgtc caatgctctt gcaatagctt ccagtgtttt
     3481 tatactggga ttacgcctgt tgttttctaa atcccctata tatgatcgtg atagacccat
     3541 taactcagca aaatcagttt gtgtcatatt tttacttttt ctaatatttt tgatgttgtt
     3601 tccaatatta aggtttttga gttcaacatt tgtttcgtct aatcccatca aataacttac
     3661 aggaacatgg aaataatcag caaggatatt ccatgtttca gtatttctag gatttcgctc
     3721 accacggtaa tagttcgtaa gagttgtata gttaatacca gtttctgtac taatttgttt
     3781 aatggtcttt ttactggaat caatataaaa ttttattcta ttcaaagtaa cgccccctat
     3841 agtaaatttt attataggat tcaataactc atagtcttta tctagtgaaa acgtatcact
     3901 atatttttct tgaatgtctt taaagacttt gtaaggtatc tctggaaagt ctttaatata
     3961 tagctcataa cctgaatcta taaggtatga agctatgtat tcttccaaag ttccccattt
     4021 aatccagtct acactagtgt ttccaatatt tgcaattgtt aaaagcctat ttttattggg
     4081 taaagacacg cctctaagcc aattattaac agaacttatt ggtgtgtcgc caattaaatc
     4141 accgaatcgt tctaatgtca gacctttgga ttcgataatt tttctaatac gttcactagt
     4201 taacgataga ttaggtttca tagctaagcc tcctcgtgag taaagtaatc tttcaatatt
     4261 ttttgtgaat caacccattt tatcgagaag tgatagttga caaatagcat tgagagatat
     4321 ttctcgctta tctggatcat caatttttga aatcacatta agataaccgg cgaagctttc
     4381 aattattcta ttgacttcct caattgacag acgttccaaa gcatccaatg ctacttcaga
     4441 tagaaaatac attgattctg ctacgcttga atcgacaccc gtcataagat aaagttcaga
     4501 aactccgcca atttctgcaa tctttttaat tctttctgag ttaggaaggt ttttaccatt
     4561 ttcccagttg cttataacag ctttatcagc tgtaggagag aatagtttgc cgaattcttc
     4621 taatgtaata ttctcttttt ttcttatttt ccttatacgt tgtccaatca ttttaatatt
     4681 tttgttgtca tcagtcagtc ctaaatctgg tttgacttct atatttcgat aatcttcatc
     4741 aggagaatac atgtacttca cctcctgaaa aatattttgt gtattgattt tttcggcagc
     4801 tgctaatatc tttgtatctt cttcgtatgt gattttttca atcgtaagtt catctagaag
     4861 agcttcacgg tagttgttca gtgtagtctc tccgtactct ttaagaattt tttcctctat
     4921 attttgtgtc aagcgataaa cataatcgga aaaatctcca tactttatcc attcaacaga
     4981 agtaccacct aatagtgcaa ttttttcaag acgctttcta ttaggtaagt tatttccctt
     5041 ttcccaattg ttaattgtgc taggactatt ggcatcaacc agtttaccta agtctgccat
     5101 agtatatcca tgtttttctc gtatttgctt tattcgatta ccagcctcaa aaagatttat
     5161 ttccattcga cacctcctaa tgtatatcat ataccgaaat catataaatc tatttcaatc
     5221 agatttacaa aacggtcatt cataaaaact agggattttt tcgtgagttc agcagactct
     5281 ctataccatc aaataaacat tcatagtatt ttatcttctc ttttaacttt gaattttctt
     5341 ctttgagtcc atctatcaat tcaaccgttt gagtaatatt ttgtaaatat attttttatt
     5401 atagaatcca ataattcata gtctttatct aacgaaaacg tatcactata tttttcttga
     5461 atgtctttaa agactttgtg aggtatctct ggaaagtctt taatatatag ctcatatcct
     5521 ataccgataa ggtatgaagt tatatattct tctaaagttc cccatttaat ccagtcgata
     5581 gttttgttac ttaattcagc aattttaatt aatctttctt tgttgggaag ggagaatcct
     5641 cgttcccaat tattaatatt tcctttttga gcatttccaa ttaaatctgc aaattgttcc
     5701 atagtcattc caaaattaga acgaattgat ttaattcttt tacctgtttc aacaggattt
     5761 ggtttcatac aatcacctct tttacagaat ttggtttcat agtatcttat tcgctctttg
     5821 agttctaagt tctctttctc caatttttct atataatatt cttgatcctg tttcttgttc
     5881 aaaaaatgac ctccaaattt cacattgtgt tacaccctag tgttacacct ccaaacctta
     5941 ttatactgta ctttaccata tgatacaata gggtataccc tattgtgaca cttacattct
     6001 acttttaatg taaaaactct tgacctttat acgcataatg cgtataataa tatttgtaag
     6061 gaggtagtcg cgaatgccac ttaccggtaa aaaaatgctc aaactagcca agaaaaatgg
     6121 ttggaaagtg gtgaggataa ctggcagcca tcatattgta gccaaagaag gattcccacc
     6181 cgttcctatt cctgttcata gtaatcagga tttaaaaaaa gggacagagc agaagttact
     6241 aaaggactta gggctgaaat agcccagtcc tttagttcat tggattaact cgaaaggagt
     6301 ggtggaaatg aaaaaaagtt accctgtaat tttttctgct gaagaaaatg gctactcagt
     6361 agtttttcct gattttgctg gagcaacaca aggagataat tttgaagaag ctatgtccaa
     6421 tgctaaagaa tttcttgatg ggattctagc ttactatatt gatgaagata tttcagttcc
     6481 ttctccaaca gatattcatc aactaaataa tttgggcaat aacaaaattg tttctcttat
     6541 tcaaagtgat ccttctccat ttatccaaaa aaagaccgta cgcaaaaacg tcacagtccc
     6601 agaatggtta gtagctagag gggaaaaaga taaaataaat ttttctaaag ttttgacaga
     6661 tgcgttacta aaaatatacg agtaaacgta aatatattaa taacaattta gttgcctaat
     6721 gacatagata gcggaattaa aagtaaaata gtactcattt attgcaggtt cccttagaat
     6781 caatacaata aatgagtact atttagactg tttaactact agtaagatat agcgatcatc
     6841 agtttcaggg ttatcccttg gtgaccatag ttttgcgtat ttttcaactc gttgccaaat
     6901 caacgattct atttttttat ttctacttaa ctccaaaacg gagtaccttc tttttcttcg
     6961 ttagctccta tttaagagcc atgtcgtttt atataactct aaaacagaaa ctactgatca
     7021 attcaataaa gccttatttg agaactgtgt tgttttacat ggttttaaaa cctgatttcc
     7081 cgcaaacggt ttatccgtaa catttgagaa ctgtgttgtt ttacatggtt ctaaaacaga
     7141 ttagggaaga tcaatctatc aatctatatt tgagaactgt gttgttttac atggttctaa
     7201 aacctcaaca atattcgtcc ttccgtctgg ttacacaact tgacagagct aacccttcag
     7261 tgagtgagtg tcccctcact atttcgttgc caaatcaacg atttcttatt ctactcactt
     7321 ttaacttcta ttccatatct tttcccttta tttattattt gttctttcaa ctgataataa
     7381 gcccattccc cataattttt atctggttca gaaatacaga ttatttgcgt cttattgcgg
     7441 atagctgttt gaataatttt tgccgaacat tgattattat aggttttaac aaaattgctt
     7501 tctcttttct ttatggcttc ccatctatct tgatctaatt ttctttttcg accatgaccg
     7561 cctttagaaa aaactcctcg gctttgctcc cttgatttca ttgctttaat tttatttttg
     7621 aagttactta tatatttgct gtcaccaaaa tcaatcacag attgattgtt aactaaacat
     7681 ttaccgctac acaaatcagt aaacattatg gttaattcag ctccttcttt gaaatctagc
     7741 ttatgataat ctttttcaaa agaaattgtt aaattcaaaa ataaattttt cccctcaaag
     7801 attagattac tctggtttaa cttgtattga ccgtccataa tgcgttctaa agttaaagcg
     7861 ctatctctgg cttttcttgg ttttctacct aaatttactt taaacctaaa tcctttaggt
     7921 aaagagatat aaaacttgtc agctttgtga atcttaatat cccgacctct gaacggtaac
     7981 aatccatatt tatttttatt tctatagatt ctaggtgttc tttgcccagt aacaattcca
     8041 acaaaatcat tgctaaaatc cttactagct ttcattttag ctgaatctat aaaatccgaa
     8101 taatcaaagt ccaatttttt tgctatatca cgtattctgg tttgactttt taaacctata
     8161 gcttgtgtca aaacgtctct ggcttctttg tttttcgcaa ttcgtagttt agataattcc
     8221 cttttcgttt tatctaattt cttttgatcg atcacttcat tacttagtaa attatcaatt
     8281 cgctcttcaa gacttagctc tcttttaata tactcatgat ccaattcttt tatagaattt
     8341 tcgatcaatt cttcaaacat cagctgttta tataccatat tcagcgcttt tacatattct
     8401 ctactatttt tccaaaaaaa accaaaaaac gaatctcttt cctcttgtga tccatcaaca
     8461 tatacttcta tttttttagt catagttatt attgtcaatt ttagcacctc attattatat
     8521 atctggtata taataatagt aacatatttc ctagactata atcaatgatc aattgtctac
     8581 ttgtagtaat tatattaaaa acaaaaacta aaaataatat attattcctt ctgactacat
     8641 atagtcactt tatccaaaaa ataagaagtg ctttcgcact ccttattaaa tttatttact
     8701 attaattgat tcagctacaa cttcttttat tcaacttcaa aatcagtttt ttggatattt
     8761 ctatctctat ttaattccat acttaatagc tcactatcag tttttactaa aagctcacct
     8821 acactattaa tatatgccaa tttacaagaa tttcttcttt tatcttgacc tactaattca
     8881 tcttgagata cccctgtaga aaaactaatt gatagaatcc gttccttgat ttcattttta
     8941 tggttcctat aaaaatcatc gacttgcgct tcttttgaaa accgatcact aataaaatca
     9001 tcacttgctg aatatattct ttcaagggtt tcttttggat tcatctcttc ttgagatata
     9061 tccatcataa tttgtttgct tataggagtt tcagcatttt tctcttgaat agctttggga
     9121 acccaagcat attcttgcgt attattggct acaactgtat gattttcttt aacataaata
     9181 taagaaaact catctgaatt atcatagata cttacattat caaaatattt ctctaactct
     9241 gctaagtttc ttaaagaaaa ttcgtatctt tttcttatta aatcttcagg tacaccgtgg
     9301 ccacctttag caacacggct tttaaccctt tcaatcgctc tttctggtga atctagtcct
     9361 atatagatta aaaacgattc ataacctaat tttttagcat ttagaagcct ctttttatcg
     9421 ggatatggat taccgccaaa agtcgtttca tggtgaaaag attttccttc tttaaaatga
     9481 ccttcgatct ctctcagaac ttcacgcata cttttgttta tatctgattg tttattccaa
     9541 tttcctccaa aacgcctcaa aatttcatct gcattaattc tatcagtatc aagttctttt
     9601 ctttttgaat tttgaatttg ataaatcgta gatttaccgg cgccattaac gcccccaata
     9661 actacataat atggtttatc attttctaaa atcatatttt aaaatctttc tgattaatta
     9721 cttttttttg atctactcgt gtcacataac tagagagtac aaggtagaaa gttctctctt
     9781 cctcactttc tgcatttttt aaggcttcgt gaaaatcatc tacagtcata ttcaaaacat
     9841 caggatacat tttctttaat tcttttacag cctcggtcat atctctcatt cccttcttat
     9901 tagtctttat taaagttctc tgcttcagct tctagtctat ctttcaagtc ttttaaaaca
     9961 accgtaagta catggtcttc accatttttt tcaattgatt gttttaccgc ttcatctaac
    10021 ttagctaaat agtcttctat aattcctaaa ttgtttactt caataagtcg gaagtccggt
    10081 aaatttttca tccgaatacc acctttaggc tgttccttta caaataaaga tttgtaatca
    10141 ttatattcat tcccattaat aaccaattct ttcataggct ctaagtggat aaatttacgt
    10201 gaagcatagt ctttcataat aatctctccc agtagaaaat cttctttttt ctattatatc
    10261 acgttctcaa agcgttgagt tattggattg tttaaaccat caagacacat catgctgcat
    10321 tttttcatca ctaccggtgc ttttccgttt gctttaacta ccgctacatt agctaacaaa
    10381 ctaattactt ctccctcgat aagctctccg ctcccaataa caacgccctg tacttccata
    10441 cctactttaa acgtttcttt tttatttacc attattccgc tctccttcca cgactacttg
    10501 tagtcatttg gatatttttt aggggaacgg ttttccgttc cctttcatca aatagaatcc
    10561 ttcatcattt caacaaataa ttaactttca gtgtaaaaaa attcttcgta attaccatca
    10621 tgaaaaatat cccatctttg ttcgtcattc tcactgaagt aggtaaaata atatctgaca
    10681 gtatctaact tatttaattg ctctctggtt ttgtaaatac aatccaagtg atcttgcatt
    10741 cgatctgacc aaaacattct tgtctgctcc atctattatc tctccttttt attaaatcaa
    10801 gggtttgagt ctttgactac ttgtagtcta tggctatatc atacaggaat gactacttgt
    10861 agtcaatagt tttctttagt ttaattaact gtctattcgt agccaaataa tgtaaaaaaa
    10921 cgcctagttt acactagacg tttaaacaat tcaataattc ttcgttatac cccattaagg
    10981 gttgaactgc tttaatactt acttgatcgt cttctctaac ggcttctatg cttacccctt
    11041 ttgtatattg gccatctttc ttatccgtcc aaatttgata ataagtaagc ccatatcgtt
    11101 caataatctc aaagagaata gttattaatt ttttttgttt aaattttggt aattcaatac
    11161 gcaatgattt ttcttgaata tcgaactctg gatttttatt tttaagttgt tcttcaataa
    11221 tcatatagtc tgtagacgtc ttatctaaat caattaatga atctgataag attgctattg
    11281 tatcgttcat aacacatcaa ctactttctt cttgttattc catttcatat acaggagtat
    11341 taagagataa tttatgtgtg aagttcacga tttttgatac atcttctaac ttgaagcgtt
    11401 taaaagcttc ttttcctctt gttaaattat cattccaatc aaatccctct cgcaaaggta
    11461 catcgacttt tacttcactg attcctatcc cttctcttcc aattcctttt tctttaaatt
    11521 cagtttcaat aaaagtacgt ccggctttat cattatcaac acaaaggact gtttctgaag
    11581 gcaatgattt aaacttatta taatgctctg taaccccatg caatacatta cctaccttta
    11641 atccattcat gctaacaaac gtagcatttt cggctttttc tggcttttgt gtaatgtacg
    11701 acatcaaatc tatttcactt tcaaaaaaat acattttatc tggttgacca aaagttacgt
    11761 taaatcctga atactcttta gagttttctt gtattttttt ccatgtaaca tccttttgtt
    11821 tgccttcatg aaaaaatttt ctagttcctt gttccgtaca cccaacgatt tcttgatctt
    11881 ttttccataa gaacaaaaca tttccttttt tatcttgttt aattagtcct ttagataaaa
    11941 aatcttccac taaagctgta tcgattcttc tctcatttac taaataagac tttgcaacag
    12001 ataccggcac ttcattttct ttctgatatt ggtacggagc ttttttcact tttgtattga
    12061 acgaatgaac ttcataatcg ctatcttcaa gtttctgttt tgcttcttta aatgaacaat
    12121 catagtaggc tttgataaaa tcaaaaatat ctccccctaa atcctttgag ttccatttaa
    12181 aaaggttttt gcgatcactg attacacaag aatcatgatc ttctaatcta tagtattccc
    12241 ttgaatcttg ctttaaacct atcccatttt gtttgcaaaa atcaacgata cttacttttt
    12301 ttgccttttt caaatccaaa caaaaacccc ctttactaga ccatcactag taaaattacg
    12361 ataaggtaca cttgtatttt tcataataat cctctgtaca gaaaataaag tttagcccat
    12421 ccctaagaga taactcccaa aagcctatct tcttgctctt tttttctcta attatctttt
    12481 ttctaatact ttttccgtac ttcaaagcca ttttaaaagc gatttgtaca tcttcacaaa
    12541 ttacaaaatg actattatta atttgacgat attcaccttg cgtttctaaa aaatttctat
    12601 tttcttgttc tatcattcat ttatctactc catttcaaat tttgttgtat tttcaaaatt
    12661 agtcaatttc aaaatcttat tctccatttc ttcagaaaca ggtggtattt caaacgtatc
    12721 attttcaatt tcattataaa tttctctcat tgcaccgttg taagccatga tagacgtagc
    12781 ttttttgtat tccgtagaag aagtatctac tggaaaacct agcagttctt ctttatcgat
    12841 ctctaacttt tcggataaat ttgtcaattt cgtttcaatt tcttctttat gactatcata
    12901 aaattcatta atatcactat ctaatgctaa tctggaaact ggttcttcca tgttttctaa
    12961 gtattcaatg cgctcttgcc aagcttgttt gtcataataa ttagttgttt ctagctcttg
    13021 ctcaaaatct tttatcacaa cttctcgaat gggaacttcc gaaaccttat gcccaacaaa
    13081 tttatctgtc atgtcctcac tactttctaa gctattccgt tcctcgttta aatcgtgaat
    13141 agtttcaatc tttactttat caggctgtaa cactacgata actacatcct tttgtcttaa
    13201 ctgttcttcc gcaataatat gtgtatattt cccaacgtca taaaatgcct tagcagtagc
    13261 ttcatgatct ccaattgtat ttccaatgtc gttaatcaaa tctacgtcat tttcattctc
    13321 actaagagaa gcaatcactt ctctttttac aactttttct ccgtaataac taacatcatt
    13381 gtaatcattt aaaatatcat tactttcttg taatttatct attactttag aaagctcttc
    13441 atcagtaata gtgcgttcat tcatagaaat cggcttctct aattcaacat ttacttcata
    13501 tacgaaacca ttatttccgt tactaccctg ttcagcgtac attgtagcca tttctttatt
    13561 aggtgttaag tacacaccaa atccattagc tgttccgttt aaacctttat gatctaaact
    13621 aaattcatca aatttattca tacttccatg aaacaggcga taagttccgc ctgtttcata
    13681 attttgttca ttactcataa ttttttcact ccatttcaaa agaacgttct gctgttttta
    13741 cagtcggctt ttccaagtta gaattttctg gtatcttgaa agttccttca tccatttcat
    13801 attttacttt agttgcaacc aatttataag ctaccactga agccacctct ttatatgact
    13861 cattgtctat tgtatctctt aaatttgttc ctgacacaga ttcaaaatct gtaatcgtat
    13921 tttcgatttc ttgtttatga gattcgtaaa aatcttccgc agtcatacaa gagtttaatt
    13981 cttttacatc attaatattt gtttggtttt caatttcttc ttgccaacat ttttcattat
    14041 agtaattgtt agcattttca gatagattat aaaccgcttc atttataaca actgatctta
    14101 gcggttcgct taattttaac gtatcatttt ttagttcatg attctcgcaa gctttcaaac
    14161 tttctacaac aaacttttca acataatcac aattttcagg tgatcgatct aataataaac
    14221 gatcattttt attttcactt ggattctcaa aaactacacc ataaacatca catacatcaa
    14281 attgtttcgt atctgaataa tatgtcacat agttttctcc tattttagct gtaacatcat
    14341 aattgctatg agtaagctca atagaatttt ctccatactt tcctctataa taatccacca
    14401 tcgcttctaa ataactaccg tggatcgttc tatccggatc gttgattgta ctatcgtaga
    14461 aattatgaaa ttctaaatct gatttttgtt gtatctgctc tttattctca ttaggaacaa
    14521 aggaattttt tagtgcatta aatgctgttt gctgttcttc caagactttc ttatcaatcg
    14581 tacttataaa gtcaatagac gtttctctaa cttcttgcat tatctgtcta aagttttttg
    14641 gatcaatttt ttcattattt ttagtccaat ttgcaatata aggaatagta aagtctgacg
    14701 tatcaatacc gtaattgtga caaacaataa atgaagtcat ttccgcttga aactctttaa
    14761 ttggtgttga gtaggaagaa ccgtgttcat tcttattgtg aagctttttg tgagccaatt
    14821 cgtgaatact ggtagtaaga ttttgtgttc tgctatttct tgaatttaat acaatttctt
    14881 ccctaccatc aacatattga atatattcac ctctagcgct tcccatttca cctctaccag
    14941 actcgttcat atcttttata gatatattca tagattcggc caaatgattt acaccttttt
    15001 ctaattgact aacacttaat tcaccatcaa tattaaaatt ccataccctg ttaggaaata
    15061 tttttggcaa atcagcttca gttgcattag tttgggaaat atcaaatacg taacccttct
    15121 taaaatgctt gatctttttg cttcttaatt ctccattttt gattttttct ttttgctcgc
    15181 ttgttgcttc ttttaacgtc ttttcttcac cattttcatc aataaaaacc gttaccggag
    15241 catgcgcata aatgcctatc cctttttctt tcgctttaac ttgaaatcct agttttttcc
    15301 aaccatcata acttgctaca gcaattgctc cttcaaatcc ctcatcgatc aacgataggt
    15361 ttaaaggact gtaattgtgt attttagaca tgaacttggc aaactcgatc atatcttcag
    15421 ggttagactg atattgctcg attttgctgt tggaagcctt aattaaatcg tctagctctt
    15481 tttgtttttg ctctttcgtt ttcggcttcc agtttccttt tttataggcc atcttctatc
    15541 tcctttcttt tttaggtaag ttaaattacc taattaaatt ataccagata gataaaaact
    15601 cttctatcta atttcactga cttaatgcaa ataaaaaaga gataagatag ttatagtaac
    15661 catcttatcc cttcttattt taccatctac tcaactatac ttacgttttt acctttacta
    15721 tctaattgaa ctttgttatc tttagaagct actttagtaa gcaaaagttg atccaattca
    15781 tcattagaat atcctgaata aaaattatct aaaactttcg ttgtggcaac tggaattgat
    15841 aacttcaaat ccatactagt agattcataa gataattctc cttcagacga aacttcacca
    15901 aaactattta aattccaaat tggactatct aaatatttat aagataattt aacggtttcc
    15961 attgaaggtg atttgttttc attatccatt ttaattgttg ctgataccat tttccactcc
    16021 ggctctgaat attgaacacc atcgatctct tctatatccg cctgtatttt aggtatacta
    16081 tcttgaaaat cagcaatttt ttggtcattt tgaaactctt gtttttcctc attttctaat
    16141 tctgttttta cagaatctaa agtggcatta tagctatcaa tatcgctttt tttcttatca
    16201 ttaaactttt gtttatcagc gttcaatgct tgttctatta tttcttcatc actcatttta
    16261 gaaacttttt ccaatgtaag ctgttccata tccgttttaa atacttttgc tttgccatct
    16321 ttagtttgga tcaccgcttt tacatattca tttttatcaa taggcgtttt atttttacta
    16381 cttgtttcga ctatcaacca acaacgtgag tctttttgtt ctgcactatc tatgaatgag
    16441 atattatgtg cttcatttga cttagcacca gcttcttctt tgccacatgc agataataat
    16501 agcgcaaaac taaaaatcat aggtattgtt gcaataactc ttaacttttt catttataag
    16561 cacctctttt ttatcggtct gatatataat tttatattat catattttta ttccaataca
    16621 cagatactct tatataatgt cttttcatgt agtttatcaa atttctattt caaaccgctt
    16681 aaaacaaata tcaaagccca aaattttcta ctggaaactt ttagagccta aaatatattt
    16741 tttatttgat ccaaaacttc agggaaatac aaacctaata tcaacacagc acctataatt
    16801 aatagcggaa tcattttctt aataaaagag atcacagcaa aaactatcag tactgcaact
    16861 aatactaaag ctgttttggg attagaatat aaaaactctt tgactgtaga cgttacattt
    16921 aagtatagtt caatcaatac gcgaaaatct ccattactta gtttatccat aaccgttcta
    16981 aaatcgatca tatcatttcc tcactctttc caattttatt gcttctttta ttgaagaagc
    17041 aacttgttca ttactatgct tatctatata taatggggaa aagtaatttt tatcaaaaaa
    17101 gtaatttgca aaagctacat cgtcttctgt cttccaatta taaaaacagt aattcccact
    17161 aatcggtatt tcaaaaagcc cccaattctc tagctccatg ttttcttttt caaacaacac
    17221 tatgattttt tttacatctt ctatagtttc cgaatcactg ttaagtagct cacgtaaata
    17281 tatatttttt gtattactgc acactatatc gaagtttttc ataatttcta ttctccgttt
    17341 ctactaattc attcctattt caaattcacg tgcttgtctc attttctctt gctctatcat
    17401 tcgttgcgct acttcataat catttttcgc acgatacata tttaaatcat cattgattac
    17461 tctatcaatt tttctaataa gctttttatc tttaaatttt tcagaaatct tcttgaagtg
    17521 gacttcattt ttttcacgtg ctaattgtcg ttcttctaat cttttttgga aatcatcata
    17581 actttgttta gcatatttcg ttttaaattc tggtttttct ctatcaaact taacgtttaa
    17641 tttttttccg tctaatagat ttttaagttc attacttttc aaattttttt cttcagattg
    17701 ctttaaaagt ttgatttgtc ctactacagc attacccatt cgagcattaa aatctttttt
    17761 tctatttaaa taatattctt gtggattgta gcgtttaccg taggttgttt gatatagctc
    17821 atcaatttct ttagtcatac gatcatattc ttttcttttt ggattatcct ttgtcatata
    17881 atttgtgact tcatcaatct tttcttttgt ttcttcagga agctcattgt agccacgtgt
    17941 tgtactaggt atttcttttt ttaattcctt taggtacttt actaattctt tgtcatatct
    18001 tgaattattt tctacatata attgaatcaa agaatctttt acagactgtt taagttctaa
    18061 aggaacttct tttctcaact tagaaatttt ttcaaactcg ttcgttctat ctaatatttt
    18121 gctaccgaat ttaaaaatca tatcgtctaa cgtagactgt tttctcttgc ccctagcttg
    18181 taaaacacca tcatattcca ttatttcacg agtattttta cgctccatag ctgttacatg
    18241 aatatggata tgttcagtat ttctgtgaat cgttgcaaac caaaaaggat caactagttc
    18301 ttccttttct tgcatttttc ccatcataga ctttgttgct tcatatagaa cattttcatt
    18361 taattcattt gtttctggat tataatatcc ttcccttact aaaaaatcat tatcgaatga
    18421 aattacatct tggaacatca ccgatccatt attttttgct tctatgaccg attcttttat
    18481 ttttgaaatg tcttttttag tgatatatct ttttgtattg ctaaatgctc cgtttactat
    18541 ttcattatct acattttcta agatagcttt tttacgtgtc atataatcaa tatatttatc
    18601 catttcacgt aaatctttaa tcactggttg atctgtttcc tgttctattt tttccaaagc
    18661 tttttctatt ctagctaatt catttagttc atcttctgaa taatcatgat ctagtttatc
    18721 ttgcctaata gcctcttgtc tggaaatata atctacaaat ttttgaagtt ctaccatgtg
    18781 attattcttt ttgctttttc cagtttcaaa ttttgcttta agaataatgc tcgcactttt
    18841 tctgttaaag atatgttcag tcatatcatc acccaccatt ttcaaaaatt ttttctacag
    18901 gcggttttgt acttaatgct aagttgcatt agaatttttt agtgttcaat ccgttgtaaa
    18961 tcttaatgac ggtgaactaa acattgttcc ctctcccaaa cgactacttt cttgttctgg
    19021 ttcagcacgc tcttcttcta attctttact agctaatcca ctattaatta atagtttata
    19081 gacagactct tctatactat ttaattgttc actataatct ttatgtgtac tgtcttcttt
    19141 tttattaaag ttttcaagtt gctgtttttg caattctatt atcgtttcaa gttcgctaat
    19201 ttttaatttt gcttcttcta agtttttagc taactcttct ttttctggat cacggaaatt
    19261 ttgattggct tttgttaaca ttggtaaatt tgaaacaaca taacggaaca tattggattt
    19321 tgtttgaaat actgacttgt ttacttcaaa gcacttattg aatttttctt ttacatcttc
    19381 tgttgctgat actttgaaat aagggtagtt catttattta aatcactcct ttcgctaatt
    19441 aaaaaattga ataagcgttc ataaactgaa gttaactcgc taatactttc taccatttgt
    19501 tcatttgaca ataaaagttt ttcaactact aaatcaaatt ctgacttttt atagtcaatc
    19561 aaatcgatct cgctatcttg gcgaatgatt gctaaaaaat agtcattcct actaaacttt
    19621 tttccggtac gttcttgaat tttattaact tttttatcaa tctcttttac atattgtgga
    19681 tcaattcctc tgattgaaac atccatacac acacctcttt caagctgttt ttatctatct
    19741 ggtatatatt attatatcaa agtccaaagg aaaattaaag atttaaagac aagaccaagg
    19801 aattaataca aagagcataa gggaaaactg atagcacctt cctaaaggaa ggtggctaag
    19861 ttggctgtgc caactggttt tctttcaaaa tagcttcata ttttttgcta tcacaaaaaa
    19921 atccattttc gacctatttt cggtcataat atagtaccta cttttggtca tagtttcgtc
    19981 cgtagttttg gtcatagtaa ctaaagcttt tacgcccaaa aaaagagtgg caagttttac
    20041 cttgccactc tcttttctat tcacaaaatt ggtttaaatt cgcttttttt agcacttcgt
    20101 ttatatcata gcaatcataa tatttacaca acatcattcc taattttaac gcttgttcct
    20161 ctttatattt tttaagctct gcattttttt tgcgcttaat cgcttttctc ttttctttat
    20221 attccgcttc aattctcatt aattcttctt cagctttctc ttgaatagtg acaatagaat
    20281 tacacattct ctctaaactc ccttaaaata atttaaacac gatcactcaa atcggcttgc
    20341 agacttttct agttgcttta ttttttcctc aaattgtttg attttatttt cactttcctc
    20401 tattgaagat ttattatttt caatagtcgt ctgcatagaa ctgatttcat tttttgtatc
    20461 tgtttgttcg tctagggttt gaaattctaa gttttgatta gctttatcaa tatctttttg
    20521 cactacttta tttgctaaat tcattttttc aattgatttt ttctcatctt ttatagaatt
    20581 ttctatagct ttaatctgat atttaaaatt atctttttgc aaatcagaag tactttcaac
    20641 ttccaattta gcatttgatt taatatcttt gtctactgca tagaatttta tttcct
//
LOCUS       DACDCJ010000050        20452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDCJ010000050 DACDCJ010000000
VERSION     DACDCJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.050"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            160..627
                     /locus_tag="IV038_001806"
     CDS             160..627
                     /locus_tag="IV038_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP8342012.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            902..1138
                     /locus_tag="IV038_001807"
     CDS             902..1138
                     /locus_tag="IV038_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342013.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            1162..1503
                     /locus_tag="IV038_001808"
     CDS             1162..1503
                     /locus_tag="IV038_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342014.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(1496..1675)
                     /locus_tag="IV038_001809"
     CDS             complement(1496..1675)
                     /locus_tag="IV038_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAP8342015.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            1897..2544
                     /locus_tag="IV038_001810"
     CDS             1897..2544
                     /locus_tag="IV038_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342016.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            2804..3148
                     /locus_tag="IV038_001811"
     CDS             2804..3148
                     /locus_tag="IV038_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8342017.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            3153..3434
                     /locus_tag="IV038_001812"
     CDS             3153..3434
                     /locus_tag="IV038_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342018.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3537..3851
                     /locus_tag="IV038_001813"
     CDS             3537..3851
                     /locus_tag="IV038_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP8342019.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3829..5523
                     /locus_tag="IV038_001814"
     CDS             3829..5523
                     /locus_tag="IV038_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP8342020.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            5543..6721
                     /locus_tag="IV038_001815"
     CDS             5543..6721
                     /locus_tag="IV038_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP8342021.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            6684..7370
                     /locus_tag="IV038_001816"
     CDS             6684..7370
                     /locus_tag="IV038_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP8342022.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            7370..8530
                     /locus_tag="IV038_001817"
     CDS             7370..8530
                     /locus_tag="IV038_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP8342023.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            8540..9415
                     /locus_tag="IV038_001818"
     CDS             8540..9415
                     /locus_tag="IV038_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342024.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            9412..9723
                     /locus_tag="IV038_001819"
     CDS             9412..9723
                     /locus_tag="IV038_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342025.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            9713..10066
                     /locus_tag="IV038_001820"
     CDS             9713..10066
                     /locus_tag="IV038_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP8342026.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            10056..10457
                     /locus_tag="IV038_001821"
     CDS             10056..10457
                     /locus_tag="IV038_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342027.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            10450..10854
                     /locus_tag="IV038_001822"
     CDS             10450..10854
                     /locus_tag="IV038_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342028.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10866..11471
                     /locus_tag="IV038_001823"
     CDS             10866..11471
                     /locus_tag="IV038_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8342029.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            11491..11853
                     /locus_tag="IV038_001824"
     CDS             11491..11853
                     /locus_tag="IV038_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342030.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11856..12038
                     /locus_tag="IV038_001825"
     CDS             11856..12038
                     /locus_tag="IV038_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342031.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            12055..15474
                     /locus_tag="IV038_001826"
     CDS             12055..15474
                     /locus_tag="IV038_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP8342032.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            15525..16262
                     /locus_tag="IV038_001827"
     CDS             15525..16262
                     /locus_tag="IV038_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP8342033.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLILCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            16272..18563
                     /locus_tag="IV038_001828"
     CDS             16272..18563
                     /locus_tag="IV038_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342034.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            18587..20278
                     /locus_tag="IV038_001829"
                     /pseudo
     CDS             18587..20278
                     /locus_tag="IV038_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7299 a         3005 c         4230 g         5918 t
ORIGIN      
        1 agaaaaagac agaatgaagg cagtagcttt ggttgaattt gctgaggcgt ataaatcagg
       61 agaactcatt tcacgtacaa aataaaaaaa gtcggattcc tccgactatg aataatattt
      121 ccgacataag tattatatca taattggggg aatcagagga tggtactttt cgatgtaaag
      181 aaatatgaga caccaagcgc gaaagatgtt gatatggaac gcacaaaaca taatgtcgct
      241 gtgtttcttt cagcatatct atcagctaga tgtagagtag ggcaacctcg agaaccaaaa
      301 gtgacagcat cttattcctt ggttccacct tctacagctg atcatatatt tgaagcagaa
      361 agaatgctga tcgataaaga agaagcacag gaagaatttg agtatctgca taaattgttt
      421 gttcgaggct attcagcgat acagcatccg cataagcctg atgtgactga aaggcgcaag
      481 aagatattct atgatcgtta tatcaatggt ctgcccattt atgtaactgc tcaaaggaat
      541 aatactagcg aagaatcggt taaagtagaa tcaaacagaa ttatcatcca atttgcttca
      601 tcgttagaac tggttgcttt caagtagcca gtttttacac tttttatacc cttttattac
      661 caatttggtt tccattttat acctttttta taccaatcac ttacctattc aacgttgtat
      721 tatgatagtg tcgaaagatt aggaaacagg atcgacaaaa taaaatgtaa gggaggaaat
      781 ctccctcatc gtttaattaa gcttcgatag gcagcagcgg aaatattaag aataaggatg
      841 tgaatttcaa ctccttctaa attgttctta ttatctatca tccgttgctg tctattaatt
      901 tatgtattgg agggtatata aaatgaagat cgcttccgaa aaaattgatc aactaacaat
      961 tttagtaaac caaagaatcg aagatattat caaagaattc gaaggtgaaa agattaagaa
     1021 cataaagctt actttaatgg atcataagtt cgaattaaat tcgacaaaaa ggaagatcgt
     1081 tgagcttata gaagaagaaa tagctctcca gatcaatgag caacaattag ctaattaatt
     1141 tatgtattgg aggggaaatg aatggaagtc gaacaaataa agtatcaagg tatggatcgg
     1201 gaacggttga ttgatattat tgagcaacaa cgtttagaat tgatccaaaa agaagctgta
     1261 tgtaaagagt acaaaaaaca tttggaacaa gtaattgagt atcattcagt agaaaagtac
     1321 agatcagtgg tgcaaaaaaa tagagcaacg agtcctgatt ctcgtcaaga tgtatcgatt
     1381 agtatcaaga cgccagtatt taattctgtt aaatcagaaa agctagaaag tatctcaact
     1441 gctttggtag aaaaagcagc tgaaatgaac tccctagcaa atgaagttag tcgtcttatt
     1501 tagtcatatc gataacttga gagtctttgg tgattccgtt ttttatagaa ttaatggttt
     1561 gttcagcgga atatttagtc aagtatgttt cgctagttgc tactacttca ttgttattgg
     1621 acttaataac gaagtaatat tggccattag ttgcttctct tataacaaag tacaaatttt
     1681 tcaccaccta taatttattt cagcggacca ctcgctgata attaaaatta taagctatgt
     1741 ataaaatttc acaatatcaa tttgtcgctg tggcggaaag ggtagacgca ataaaggatg
     1801 aatgggaaaa gtcaatgagt aattatctga aatttacaag tcccctgttt agttatctaa
     1861 gattatttga taatattatt tctgaggtga tagaaaatgg tgggcgtagt taaatttctt
     1921 gggtttacat ttgatggata tggtacgtta ggcgaatggg taggtattat tttgatagtg
     1981 attacaattc aacaaacatc aaagtactac tggtattcta atagttctaa aattgtatca
     2041 agaataactg aaggaataac caaaaaagat attactgtcg tcggtaaata tgggtttact
     2101 ataactttat taaatgaagg gaaatctgaa accgcaataa agttttgcgg gattataggt
     2161 aaatataaca ttttccaacg ttttatcaga aagcattggt acaaatatat ttacaaatac
     2221 cctgtagtta aaaagcttat tgaaaaatat gtggaccacg aaatatctta tctcaatcca
     2281 acagacctta ttaatcaaga attccagaat attggatact tagaaaaagg tctaaaaata
     2341 ccaattgtaa atgaaagttt agttactgca atggtagcta acataaagaa cagcaagaaa
     2401 ttagaaaaga gatataaaaa atgtaaaaaa ttgaaatttt cctttttatt cttagaatat
     2461 aatggcaaag aatattatca agatatttat gttttgcgag gctcgaactt atataaagac
     2521 attaaaaata agatttcagt atgagatttt ttaaaattcg tagatcactc tttgagtggt
     2581 ctttttattt tgcgtaaagg aggcacgacg atgtatagac cacaatactt agaacagaag
     2641 catgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta tagaagacca
     2701 ataaagagcg atacatataa acggaaggaa agcaatgaag ttattccatt gtatggcaaa
     2761 agaatagcta agtattaaat aagattgcga aaggagatgg gacatgaccg aggaattcta
     2821 tagatggtta ttacagttga taagagaaga tcgtttggtt aagttctatc agtctcctaa
     2881 atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag
     2941 aagactaggt aagtatcata gagtagagaa cgttcatcat ataaaggaag tcaaggatag
     3001 acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga
     3061 agttcatggc agatatctta cagcgttaga taaacaagag aagaagatag aaagcttcgc
     3121 taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgacaat ggcagagagt
     3181 atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt aaacgtttga
     3241 tatacgttcg ctatgtaggt attagggatc tgttatcaga taactgttgt agtaaataca
     3301 aagtgaatca agtaagagaa gcgttgaata aagataataa cgtcgaaaga ataaaaaatg
     3361 tttttggata tagtattgaa gagattaatt attacattga cttcgctgaa gctttcattc
     3421 cgatggtgag ataacccccc cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg
     3481 agggggctgt caggaaaaga gattttttcg aactttatca tgaaaggagg gctaaaatgt
     3541 ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt acgccgctct ttaataagcc
     3601 aattggaaag tcagaaaaca aatattgagc cattcttaga taatgttgat cgttatatca
     3661 gtttatggga aacggcgata tcactggaag aagatatatc cgagaacggc attagactgg
     3721 agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat
     3781 tgatgttgga taaacttgcc attactcctg aattggtagg tgaagcaaat gaatcaattc
     3841 ctgagttata agcatattga aaattggttc aaagctatag aagaaggcac tatcaaggta
     3901 tgcaaagagc aattattgct aaaaaattat ctagaagaaa gagtctttac tagagaagat
     3961 atttacttcg ataagcagat ggtagaggat tcaatcaata taccagcaca atactttcca
     4021 ttcgaattaa ttccgtggga aaaatttcta caatgtttta tttatggtgt tcgatggaaa
     4081 aaagataaaa cactagtgtt caatagatat ctttcattaa tgggacgtgg taatggtaaa
     4141 actggttttg cttcttggaa caacttcttt ctactaaccg ctaaacacgg tattaaaaat
     4201 tatgatattg atatctatgc caataatgaa agccaagcaa agactagttt tgatgatgta
     4261 tttaaagtaa ttaaagatca tcctgattta gataaaaaag tatttaaagc tacgaaggaa
     4321 gttattcaaa atatcgctac aaacagcaaa cttcgttata acacggcaaa tgctagaaca
     4381 aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat
     4441 tcaatgataa atgtggctac ttctggtggt ggtaaaattc gagattatag agaattttat
     4501 gatacaacta atggtcatgt tcgtggtggt ccgcttgatg acattataga agaatcaaaa
     4561 atgattcttt ctggagaact tggaattgac aaggatggag cagaattttc tagtttgttt
     4621 ccatttattt gtcgcttgga taacgataat gaagttgatg atcccgacat gtgggaaaaa
     4681 gcttgtccaa ctattaatta caatgcagat ttaaaacgga aaatgtttca agaatactct
     4741 caaatgcaac gtaatgctgg tttaagactt acgttcatga ccaaacgaat gaacagacct
     4801 atggaagata cacgatttgc tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa
     4861 gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg attttgctga tagacgagat
     4921 tttgccagcg ttgggttgct aggaaaatac gataaagatg tttattttac acaacatact
     4981 tttatccacg aatcagccct tcgattacaa aacatcaaac gagaggttat agatatttct
     5041 atagatcaag gaaaatcaca gatcgttcat ggaaaaaata tagaagctga ttatattgta
     5101 ggttggtttc ttgaaatgag taataaatat tatattaaaa aaatcgctat ggatatgtac
     5161 cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt ttactgtgga aattgttcga
     5221 agcggatctg ttacacatgg tatgttaaaa gatctggttg atgacctttt tattaatcaa
     5281 cgtttatttt ttggtgacga tgcgattatg cgttggtatt gcatgaatgt atatgaagag
     5341 catatttcta atggaaatat acgctatgaa aaaatagaac ctgaaactag aaaaacggat
     5401 ggcttttttt cattccttca tggtttgaat tttttagatg atatttatga ttctgctcct
     5461 gtaacagtca caaatagctc agtagaaaat acaggaactg gatttactcc tctagtattc
     5521 taacttgaaa ggaggtgaga aagtggggat ttttcaaaag gcggtaggat acttcacaaa
     5581 aaaagcaacg gttcctttag aagaatactt ttgtaaattg caagttgatt ttgtgtatcg
     5641 aaaatttgcg attgaaactt gtattgattt gattgcaaat gcgatgagta aagcggaatt
     5701 caagtcatat gaagatggaa aaaataaaaa gaatgatctt tactataggc tgaatgtagc
     5761 tcctaataag aaaaataatg caacagaatt tagaaaaaaa ctgatcagga gattaatatt
     5821 ctacaatgaa gtattgatcg tttctccgtc taataattct agcgaaatat ttattgcgga
     5881 tagttgggat gtcacagaat atgcattgaa agatgatgtg ttttctcaag tgcaaattaa
     5941 caacatagtc cttgatagag aatttctaga aagtgatgtt atctatataa aatacgcaga
     6001 tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg tatgggaaac tcatttctag
     6061 tgccatgaat gtttacaagc gctctaacgc tcgtagatac gtactgaaag ggaatttatt
     6121 ccgaccgcaa gacaatacaa cacaagatca aatcaataaa atgatgacat cacaatttaa
     6181 ggcttttatg gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt
     6241 agaagatttc agcggaaact tccaaagcaa ttcaagagat ataaaaaact taatagacga
     6301 catctttgag atgacagcag cagcgtttca cgttccgaaa aacctactaa agggagacat
     6361 gagtgggtta tcggatcaag tggacgcttt tttaatgttc gaaatcatac cgattgctga
     6421 acttattcag gatgcgttta acgctagtct ctatgaagta gaagaatact tgtcagggaa
     6481 ttttgtacgt gtggatacaa ctatgatcaa gattactagc ttcaaagatt tggttgacgc
     6541 tattgatgta ggcattagaa atggagtatt tacaatcaac gaaggaagag agcgcgttgg
     6601 aaatgatcgc tctgataagg cgatggcaga tgaaatattt ataactaaaa acaatcaaca
     6661 agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac atttctagca gtaaagaatg
     6721 aaggcgcagt accgcaaatt tttattcagg gatttattgg ttctagttgg ttctttgaag
     6781 ggaatactga caagggaatc aaaaatattt tggatagtct aggtgatcaa gaagaaattg
     6841 aagtagtaat taattcaaac ggtggagacg tatttcaagg gattgctatt gggaacttac
     6901 ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg cttagccgct agtgctgctt
     6961 caattatcgc aatggctggc gatactataa aaatctacaa caatgcacaa ttgatgattc
     7021 accgcgcttc cacatacgga gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac
     7081 tggaatcaat tgataaatcg gtaaaggctt catataaaac acgattcaat ggcacagatg
     7141 aagcattgca agaacttctt gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt
     7201 atggattggt cgatgaaatt atcgatgcag aaaatagctc aggtactgaa gctaaaaaag
     7261 aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg
     7321 catttacagc agcattaaat aaaacatttg gacaaggaga tgtaaaataa tgacagttaa
     7381 aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg aaagctttta aagatggtaa
     7441 cgaagaatct tttagcgcag ctatggtaag cttatctaag gaaattcagg ataaaatttt
     7501 agaagaagca acagcaaaaa atcaagatca attagtatta atgaaccgtg gtcagcgtgt
     7561 attaactaca caagaaacaa aattctataa cgaagtagtg aaaaacgaag gttttgcagg
     7621 ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta tttgaagatt tagaacaatc
     7681 tcatccacta ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag aatggattgt
     7741 gtcacgtgga gtcaatccag catggtgggg taaactttgc gaagctgtta aaaaagtttt
     7801 agataatggc tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt atattcctgt
     7861 atgtaaggca atgcttgatt taggtccagt atggttagat cgttatgtcc gtactgtttt
     7921 agtagaatca ttgagaattg cattagaaca agcaattgtt gatggtactg gtaaagatat
     7981 gccagtcgga atgatgcgtg acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa
     8041 agcagaacct attacagctt tagatgctgt aactatgggc ggtttgatgg cgcgactatc
     8101 aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt
     8161 ggtcctaatt gtgaatccaa ctgattactg gtctaaagta ttcccagcta agactgtact
     8221 aactgctaat ggagaatacg tacaagtatt gccagtacca gtttcagatt tgcagtcaac
     8281 ggctgtgcca gaaggaaaag cagttattgg ggtagcctca gattacttca tgggtgtagg
     8341 atctacacta aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta
     8401 tctagctaaa caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga
     8461 tattagcgct ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac
     8521 acaagcgtag gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa
     8581 atgggagctt gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt
     8641 gcttttcagc agacacgatg atcagattcc tatttttttt gagaaggaat atggtgttga
     8701 agttcatgtg tacgatgatc tgcgggacga caaagagtat attccttcga ttaaaccata
     8761 tttatggtgg aaatatttag aagaagatca ttcgcgtgag gacgaccgat atttctatat
     8821 cgattcggat gtcattttca ataaaagaat taatttgcgc aaattgcctt ctaaagatga
     8881 tgtttggtat tgtagcgact gctgtagtta tctaagtctt gattatatta gaagctgtga
     8941 aaacggagaa aatattctaa aagatatggc aaacattgta aatgttacag tagaatcttt
     9001 ggaaactata aacactaatt caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa
     9061 ttattggaaa aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa
     9121 aacaaatata caaatctgga cagccgagat gtgggcacag ctttggaaca tgatgtattt
     9181 caatattggt cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga
     9241 aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt
     9301 atttttcaaa gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa
     9361 caagaaaaaa tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct
     9421 gaacaagtga ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac
     9481 gaagaagatg agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa
     9541 gtgagtacaa cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga
     9601 tgtagatacg actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta
     9661 ttaggtttca ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa
     9721 taatcgtaga ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac
     9781 caaaagaaat gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa
     9841 atttagaaat ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa
     9901 attaactaag aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca
     9961 atattttatc gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt
    10021 ttatatctat ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa
    10081 aagaacgttt aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc
    10141 tcaaactctt tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca
    10201 ttctcgaaac aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc
    10261 tatatctaac tttctattca gaaaatagag aagatttaac aggagattca ctagatatta
    10321 tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat catttaaaac
    10381 tagagaacca agatcgctat atcgatcaat tggtatttac gtttagacga ttattgaaga
    10441 gtgattgtca tggctaaaaa tagttgggag ctaaaaataa atggacatga tgaacttctt
    10501 gtgcggatgg aacgctattc aagcgagagc gaacgactga ttaacgaagc attgaaatca
    10561 aaaggttcgg ctattgcagt ggataggatt acagaaaaaa ttcctgtttc tgaagcagat
    10621 ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca atacattaat
    10681 ttgggtttca tcattagacc tacaagaaaa tttgagtatt taaaatatcc tgatttgggg
    10741 ataggtactt ctaaaagaaa tcagccggac gaatttatga gaagaggatt aggtcttgca
    10801 cttgatccaa ttacagaact tctgattcgt caattcgata aattaaataa ataggaggaa
    10861 caacaatggc taaaacaaca acagtaacaa cttttgacaa tataagtatt aaaagaattt
    10921 cttttaattt taagaatgca acaaatgcaa tttcaactga ttgtaatgga caattagatg
    10981 gcgaaacaga aatgcagaga attgtaaaaa aatgcggttc aacagaagta aaatcgaaat
    11041 ctaaaccaat caatatgacg gtaacaatta ctgcacatgt accgatggaa gtttatcgac
    11101 gcttcaatgg gttgaaacaa gatgaacgta ttaaatcggg aatttactct tatggtcctg
    11161 attccgtagg ggaagatttc tctcttgctg cagagatcgt ggatgatttc gaagaaaaaa
    11221 ataagctgat tggtatgtta gcatgcactt cgaatacagg attaacattc tctattgaaa
    11281 atggtgcgga tgaagtagct gcgttagaac tagaaacaaa agttatgcaa gatgaatttg
    11341 gtaaatttta tcatgaagca attgttgcag aacttgaaga agacttaaca gatcaatgga
    11401 tgacgaatct atctgctgat gtgattaaaa aaacttcagt tgtgacaact acggccactc
    11461 aatcacagta aaaaaaacgg aggtagcgaa atgaacgaag attactcaaa aattgaacta
    11521 aacgatggaa caattttgaa tttagaacct aaactgaata tcaagaaatt attgatgatc
    11581 aatagagatt ttaacacaga cgagtttgca aaaatgactg tgggaaaagg atccatggat
    11641 atttctgtta ttcaaggtgc aaaggctgtg tatattgctt accgccaagc gaacatgact
    11701 gattatattt cattcgatga atttatcgat aaatgggatt ttgatatggc tactgccagc
    11761 tatatttatc aattgatgat gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa
    11821 aaagcaaata aggaaaaaaa gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt
    11881 gggtcgatgt ctattcgatg ttgaccgacg ttttttctat gccttcagat ttggttttaa
    11941 gcgatatctg tttagatgac attttgcaaa tggcttacaa caagagtgct tatgaaggat
    12001 ggaaaaacta tgcaataaac caatcccaga aaaactaaag aaaggaggta aaaaatggct
    12061 aaaaagagaa cagaagcaga agtaacattc atagctaacg atgacggatt gaaatctacg
    12121 ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact agaacaagct
    12181 caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc ttctttagaa
    12241 aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gccaacgttt agccaatgcc
    12301 aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgagagaga actgattaac
    12361 caacaaacag cgcaacaacg tttgtcaaac gaaattgata aaacgagtaa tgcactagct
    12421 caagcaaaag gcgaaataca gacgtacgag tctacaatgc aacagttgga tagtgaacaa
    12481 aaaaatgttc aagctagtgc ttctctgatt gaatccgaat acaaaaaatg gcaagcaact
    12541 gctggtcaat cagcttctga atccgagaaa ttagcgaaag cccaagaata tgtttctcaa
    12601 caatctgaaa atgcagagaa aacgatagat atcctgagac gacagttaga agctacacag
    12661 tctgagtttg gcgctacatc cacagaagca atgcagatgg aggcgaagct taatgatgct
    12721 gaacgtgaat ttgaagagtt aggacaagct gctaaaaatg tagatacaac taacttggac
    12781 gatatcggaa gcaaaataga tatgaataat ctaatggaag cttctgacgt tttaagcgac
    12841 attggcgata agcttacaga attagggaaa caagcagtgg actctgctaa tagtgtaggt
    12901 agttcccaga gtaaaataca agctaatttt ggtttgacta aacaagaggc tgaagaatta
    12961 acgaatgtag ccagagacat ttattataaa ggttttggag aatcgttaga tcagtccaca
    13021 gatgcattga ttttggtaaa gcgtaattta ggcgatttaa ataatcaaga tttacaaaat
    13081 atcacggaac aagctatggt cctagaaaac accatgggcg ctgatatgga tgaaacgtta
    13141 cgtggtgtaa atggcttaat ggtcaatttc ggcttgagcg ctcaagatgc aatggattta
    13201 atggtttcgg gtactcaaaa cggtttagat aaaacgcacg aattaggcga caatatggca
    13261 gaatatagcc aattatggag tcaaatggga tattcagctg atgaaacgtt cggaatgctt
    13321 caaaatggtt tagatgcggg tgcttataac cttgataaag tcaatgactt agttaaggaa
    13381 atgggaatat cgttaacaga tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt
    13441 actagaaaag cttttgaaga gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat
    13501 tccatgattc aagattttag caatatggat ggtcaatacg accaattaaa taaagcttct
    13561 acaatttggt ctgcacttgg cgaagataac gcgatgaaag ttgtccaatc tttgactgat
    13621 gttaaccata catttgatga tgttagtgga tctgcacaaa aaatgaatga agattctact
    13681 actccgttac aagaattaaa cggaaaaata gctgaattaa aggattcatt agctcctata
    13741 ggcaacacaa tcatagatgc actcgaacca gtaattgatt ttctaggaaa gatggctgat
    13801 gcgtttaata atcttccaca accagtacag gattatgccg tagcgattgg cggattgact
    13861 gctgcattta ctttattaat gccaataata gttggcttca tggctctagg tggtcctact
    13921 acattaataa taggagcagt tattactgtt attgctggag ttatagcaat tataaaaaac
    13981 tggggcgcaa ttactgactg gtttaaggga atatggagta aattcactga ttggttgggt
    14041 ggtacttggg aaagtataaa agaaggtgcc tcatcagttt gggatggagt taaagaaacc
    14101 tggtctggat ttgtagattg ggttcaagat atttggcaag gagtttctga ttggtttgga
    14161 gagctatgga gcggattagt tgaaggagct tccaacatct ggcaaggagt ccaagagact
    14221 tggcaagcat tcgttgattg ggtttcaaat atttggaacg gagtcaaaga agtatggtcg
    14281 attatttggg cagacattgt aggaattgtt caaataccat ggaccttaat aacgtcattg
    14341 attcaagccg gtattaatat tatcgtgggt atttttgatg tagctggaca gttattaggc
    14401 gcagcttggc aagctgtttg gacacctatt tctgatttcc ttaaaaacac ttgggatact
    14461 atgacacaat gggtaagcat cgcttggaat ggaattgtaa ctacattcca tactatattt
    14521 gatccagtag tggcatggtg gaatggtata tggacagcta ttagtactac ggcttcaaat
    14581 atttggaatt caattagtgc aacagcttct agtatttgga acagtatcaa gaatacaatc
    14641 actagcttgg tacaagcagc tgctacagta attcaaaata tttggtcaac tgtatctagt
    14701 tggttaggtg gaatttggaa ttcaatcagc tctacagcat caaatatctg gaatagcgtg
    14761 actagtagta taagcaatgc tataaacgca gctaaaagtg ccattcaaag tgtttggaat
    14821 agtatatctt cgtggatcag cggaatttgg aacggtatca aaaacactgc tttgaatctt
    14881 tggaatggaa ttacaagcac tattagctct aaagtaaacg atggaaaaaa tgcaatttca
    14941 agcggttggt ccaatctaac aggtattgtt tccgacatat tcaataatgt taaaagtaca
    15001 attgctaaca tttgggaagg tatcaaaaag actgttagcg ctccaattga ttggatcaga
    15061 gataaaatca gtagtatctt tgataatttg aatatttcta taccacatat tccgttacca
    15121 cattttaaat tgagcggaga attcaatcca ttgaagggga aaatcccaac gttgggtgtt
    15181 gattggtatg cgaaaggtag tgtgtttaat tctccgaata ttatcggtgt cggcgaagca
    15241 ggacctgaag cagttttacc tttgaaaaga tctgtgctgc aagaaattgg tgatcgtatc
    15301 ttgagtagca catcagtttc atctagggca caaacgattc aacctgtgaa taactacgaa
    15361 ttcaatttca caattgatgg taacgcagat gaggttacta tgaagcaaac aactcaacaa
    15421 atcattgata gcattacaaa agttcaaaat gataatgctt cggcatggcg ttaaacagga
    15481 gagtatttct cctgtttttt tagtattaaa aaggatgtga aaaaatgact gattgtatac
    15541 attctataat cgatggattt cctgattatt tgcataaatt ggctttagct gaaagaccaa
    15601 ccataccttc tccaaaaaga cagagagttg aaacttctgt tttaggaagg ttaggtggct
    15661 tagtacaaga ttactcgttt gaagacatgt cgtttacatt gcactataac tatttagagg
    15721 atgtggaaga ccatcaagcg ttcaagcaat cgttttatat catgcgtcat tggttaaatt
    15781 atgcaaagaa attagaattc tctgatgatc ccaacgtcta ttatgttatc cagactatcg
    15841 atattgggga tgcagaaaac gatattgttg aatggggaga gttcgatgta aatattactg
    15901 cgaaaccatt cgcaagagtt caagaagatg tacctataac cgtagataaa ccacagtcat
    15961 ttaacttgct gaataatagt ttagaagaaa gttttccaaa gattatcatc actccttcag
    16021 ctacttcatg ccagttcatc ttaaatgatt atgtgtttag ttttgaaggc ttagtagtag
    16081 gaactgacgt agtcattgat agtgatttga tactttgcta cgaagagcaa tcggacggag
    16141 atattttaga tcggtccaac aaaatgaaga ccatgcaata tccgacattg caagtggata
    16201 ttaattattt taattgtact ggtttgagca aaatacaaat ttatcgtaat gggttaaggt
    16261 aggtgaaata gatgatcgat aatttaataa ctatttacga taaaaacgac gcgaataatt
    16321 tagctgaaca tttatatgat acgcaaggtt taggcgcttt gtcagactgg ttaacagcta
    16381 ctgttagcaa taaattaaac ggagccgaga tatttcaggg tacttatcca ataagcggaa
    16441 ctaatgcaga tttgattgta gaaggacgta ttattcagtg ttatgtagat gaaaatcgag
    16501 caaaacagcg tctacggatt tattatgcaa agacttctgt aataggcaat acgatagaag
    16561 taaaagctga acctattttc aatgatataa gaaaatcggt gttgaataaa tatgacagtg
    16621 gaacagaaaa gatcactgct agtcaggcat ggcaaaacgc aaaaacttta gcgaaaccag
    16681 ttattccttc acagttttct ttctcgtcat tagtagatac gcttgctaat gtgaagatag
    16741 aaaaggcgaa ttttttagaa ttctttggtg gaaaagaggg atctattcta gatcgatttc
    16801 atggggaatt tctaaaagat aataacacat tacgtcatga aaaaaggcta ggcacggatc
    16861 ataaaatcaa agcgatttat actaaaaact taactggtct tgacttagag attgatgctc
    16921 aaagtgtttt agttggagtt tatccattca ttagcagctc ttcagaagga gaagacgaga
    16981 tcactctacc agaagaagtt attttcacgg attacgtgga tgattatcct gctggatatg
    17041 tttcttttgt tgattttaaa gacaaagcga ctgatgtagc cacattaagg gaagctgcta
    17101 aagactggtt gaaaacaaac atagataaac aaaaaccaca agtgagtggt tcgattgaat
    17161 tagtaccatt gaggcatcaa agaggctatg aaaaatttgt tgatctagaa aaagtttcga
    17221 tgggtgacgg agtagatgtg tatcatccac agttaaaagt gaatatgtca gcgagaattg
    17281 tggaatatac gtttaatgtt ctaaccaatt catacgataa attagttgta ggaaacgtca
    17341 aaacaaactt cttagaaaac acagagaata atgtcagcaa tttgattaat gatgccattg
    17401 atcaattgaa aaacggtggc gaaatcagtg atttactcaa tgatattgta gatcatcaaa
    17461 ctgatatgat tactggtcaa aatggtggtt atgttttatt agatcctaaa gaagcgccta
    17521 gtcgtatttt gattatggac acaccagata agaataccgc aaggaatgtt ttacaaatca
    17581 acaatgctgg tattggtttt tctaaaactg gcattaatgg aacatatgaa acggcatgga
    17641 cgttagatgg cggattcaat gcctcgttta ttacggctgg tgaaatagta ggaattacta
    17701 ttagaggtac tacattaatt agtgatggtg ctgattatag aacaagtatt gctaatggca
    17761 aaatgacttg gtactcaaaa aaagttaaca aagatattat ggagctagaa gcacgtgatt
    17821 atgtaagtgc tgatgccggt attgtatcat acaccatgaa aactggtggt ggtttcatga
    17881 ttagaaatcc acagggtaac ttggttttta gtacgtggga taatggtaat aacagaccgt
    17941 ttctatcttt tggtgcgccc aatttcaggt atagcaatgc tagttatgta acttctggcg
    18001 acggtagttc tttaagcatt aatggtagtg cgggtaactc atgggaattt aaggtagctg
    18061 gtaggactat gaaatttact agtgatggta tgctaacgtt accaggttgt ttttttggtt
    18121 catgggaaga tgggaaactt gctaggtttg aacaatcaac ggtacaagta tataaagatt
    18181 ttactgttag aggtactaaa aactcaactg taccaacaga acattatgga caacgactat
    18241 tgaacgctta tgaaactcca gaatattatt tcgctgatta tggggaagcc gttacaggtg
    18301 acgatggtaa agttcgtgtt gatattgacc ccatgtttgc tgaaacagta aatctaagtc
    18361 ggtatatgac acatgtgaca cctacagaac tagttttgtg tgctgttact catgaagata
    18421 ttgaccattt catcattgaa actagtaagc caaacgtatt agttagatgg aatttagtgg
    18481 cacaccgtct agggtatgaa gatattagat taaaagagga tacagcatat gatagcacag
    18541 tgcttgacca aaaacgtttt taaaacgaag acaaggaggt atataaatgg ctagcagttt
    18601 atataatttg gctttagatt tcagcaaaga attaaactac accaaagcta ttatggctcg
    18661 tcaaggtgat aaagggatta cggtgacggt taaaccgttt ctaaatggct tgcagatgga
    18721 tacgagtggc ggaacattta ctttaaaagg aacaacacca tctaaccgtt acgtagataa
    18781 tgttgcaact agtgtaacta gtgaagaagt cacgttttct cttgatggca catttatgag
    18841 tgaagcagga tattataaac actgctacgt agaatataga aaagacaatc aaattttaac
    18901 aacgcaagat atcatttttt tctcactagg agtgtctgac atttcgcaag gccaagccga
    18961 tgaatatgtt tcgcaattag aagagttgat tcgaaagtac aacgaaactt ttgatgcttt
    19021 tatggctgaa atcaaaggta gagtggatag cttaaatcaa cagattactg atttaactgg
    19081 tcaagctaaa acgctacaag acaagttaga tgctctgaaa gaagaaattt ctaagttagg
    19141 taacttacaa gtgatgtaca gtaacagcat cgacttcggg ggatatgatt atagcggtaa
    19201 gccgaattta atgtccaaac taaaatcgag cgattttaac gttggttacc acgggtcact
    19261 aactttggat aacgaaaagt tacattttac ttctgatggt acaggaagca ttattatgtt
    19321 tacgcgtatt aatacacctc agcttactag tgggaaaacc tatactctga gtgcgaaagt
    19381 tcgatttgat gaaggaacta caggagctat tgataaatta cgtttggtgt atcgtacatc
    19441 accaggagaa aagatattat tggaagcaaa tagtacaaat attacaacag atgatgtagg
    19501 gaaagaaata acaatcaaag gtacagctaa cgttaattat caaatcacaa atttagatcg
    19561 attttatatg agtattagct ttgttgacag ggataaaata aatggcggat ttaagttgta
    19621 cgacatcaaa atcgaagaag gctcaacagc cacaccgtac cagcctaatc tacttgatgc
    19681 accgtattat ttgagtaagg tggctttggg tgagaatcta attaaaccag aatcacaaca
    19741 accagttact aatagtaact atcttattaa cacctataac attaaaccaa tggtaaaagg
    19801 taagaagtat accatcacac ttgaaggaac taagccagca acacaggttt ttagaccatt
    19861 tttcacacga gcaacaggag atgcatggga ggttggtgac ttacaaccag tagaaggctt
    19921 aactaatgtg tggtctaaaa catttacagc agcagatgac tcacacccta ctaccccaca
    19981 agtgcagatt tatcaagtac caagcacaag tgtaggacaa tgtacaatta agtggttaaa
    20041 actagaggaa ggcaatactc gaaccccgaa tattagtgag tataaatatc gtggtactgg
    20101 tatgcgtgat tcaaacaatc caaaagatta tgtttgggat ctagcaccag aatatgtcga
    20161 agataatttg gccacagata ttaaaatttc tgaaattact ggtaaagcaa acaattatac
    20221 cgatgggaaa gtatcggaga ttaattcgca gttgactgct tcaattaatg aagtagacac
    20281 cacagctaag gatgctcaaa caaaagcgaa tgctaatgcg actgctatag atgaattaga
    20341 caataagatc gatgaacgca ttaatgatac agctactacc acattaacag ttacaaacgg
    20401 gaataccgga tcagcaaagc tttatcgtga aggaaaaaca gtttctatat at
//
LOCUS       DACDCJ010000051        20439 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDCJ010000051 DACDCJ010000000
VERSION     DACDCJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.051"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(3..1157)
                     /locus_tag="IV038_001830"
     CDS             complement(3..1157)
                     /locus_tag="IV038_001830"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP8342035.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1342..2577
                     /locus_tag="IV038_001831"
     CDS             1342..2577
                     /locus_tag="IV038_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HAP8342036.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKSFISLRHLNGAVN"
     gene            2577..3782
                     /locus_tag="IV038_001832"
     CDS             2577..3782
                     /locus_tag="IV038_001832"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP8342037.1"
                     /translation="MEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIPMGIAQN
                     FQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKSEYQAVI
                     NAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDVKDAMGA
                     NMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNKEIGEQI
                     AKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAARNGLYQ
                     GLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQVIAAVG
                     LAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKMNQQTAI
                     QILEKIREK"
     gene            complement(4112..4816)
                     /locus_tag="IV038_001833"
     CDS             complement(4112..4816)
                     /locus_tag="IV038_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342038.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(5003..5248)
                     /locus_tag="IV038_001834"
     CDS             complement(5003..5248)
                     /locus_tag="IV038_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342039.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5265..6116)
                     /locus_tag="IV038_001835"
     CDS             complement(5265..6116)
                     /locus_tag="IV038_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP8342040.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6282..7286
                     /locus_tag="IV038_001836"
     CDS             6282..7286
                     /locus_tag="IV038_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP8342041.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(7527..7883)
                     /locus_tag="IV038_001837"
     CDS             complement(7527..7883)
                     /locus_tag="IV038_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8342042.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            8251..9438
                     /gene="tuf"
                     /locus_tag="IV038_001838"
     CDS             8251..9438
                     /gene="tuf"
                     /locus_tag="IV038_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP8342043.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(9626..12217)
                     /gene="mprF"
                     /locus_tag="IV038_001839"
     CDS             complement(9626..12217)
                     /gene="mprF"
                     /locus_tag="IV038_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP8342044.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(12243..12962)
                     /locus_tag="IV038_001840"
     CDS             complement(12243..12962)
                     /locus_tag="IV038_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8342045.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            13171..13803
                     /locus_tag="IV038_001841"
     CDS             13171..13803
                     /locus_tag="IV038_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP8342046.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            13862..14722
                     /locus_tag="IV038_001842"
     CDS             13862..14722
                     /locus_tag="IV038_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP8342047.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            14829..17222
                     /locus_tag="IV038_001843"
     CDS             14829..17222
                     /locus_tag="IV038_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8342048.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            17404..19245
                     /locus_tag="IV038_001844"
     CDS             17404..19245
                     /locus_tag="IV038_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP8342049.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            19269..20378
                     /gene="rpoD"
                     /locus_tag="IV038_001845"
     CDS             19269..20378
                     /gene="rpoD"
                     /locus_tag="IV038_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP8342050.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6587 a         3655 c         4256 g         5941 t
ORIGIN      
        1 tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct
       61 gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat
      121 tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga
      181 tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg
      241 tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa
      301 gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg
      361 ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg
      421 ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa
      481 tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg
      541 atatacgtag aattagataa aggaccatca acaacaggaa attcgctata atctggacgc
      601 cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc
      661 gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt
      721 gccaagccgt aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt
      781 ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact
      841 tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct
      901 tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct
      961 ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca
     1021 cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca
     1081 cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga
     1141 tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag
     1201 gtatcataaa aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt
     1261 aatcttagaa aaaaacaact ggaacaagag ctatttgttt ttacagcaaa gaatggtata
     1321 tttttgtagg aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg
     1381 ataggaaagt atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg
     1441 actaaaggat tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattc
     1501 ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc
     1561 ggtctccctg tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa
     1621 gcagtgatct tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc
     1681 ggaacagaga gtatgtctca agcacctatg ctgaaaccgt atcagtcaga aacaaatgaa
     1741 tatggtgaac caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca
     1801 catatgggat taaccgcaga gaaggttgca acacaatttt ctgtgagcag agaagaacag
     1861 gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt
     1921 tctgaggaga tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca
     1981 gttcgtggaa atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa
     2041 ggaactgtaa cagcaggaaa tgcttcgcca ttgaatgacg gtgcctctgt ggtgatcctt
     2101 gcatccaaag aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta
     2161 gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa
     2221 ttgacagata gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca
     2281 tttgcagctt cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat
     2341 atatatggag gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg
     2401 actacattag cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt
     2461 attggtggag gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag
     2521 gatgtgcaaa aaaaaagttt tatcagctta cgccatctga acggcgcagt caattaatag
     2581 aaaaaaatgt attgacacaa gaaactgcgt tgatttttca ggaacaaaca ctttctgaag
     2641 agctctccga tcacatgatc gaaaaccaag tgagtgaagt ggaaatccca atgggcatcg
     2701 cgcagaattt tcaaatcaat ggtaagaaaa aatggattcc gatggcaaca gaggaaccat
     2761 ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg aaatatctgc gcagaaacac
     2821 ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa atcggagtat caagcagtga
     2881 tcaatgcagt taatcatcga aaagaagagc tgattctttg tgcaaatgaa agttatcctt
     2941 caatcgtgaa acgcgggggc ggtgttcaag atatctctac tcgggaattc atggggagtt
     3001 tccatgcata cttatctatt gattttttag tggatgtaaa agatgcaatg ggcgctaaca
     3061 tgatcaactc gattcttgag agtgtggcaa acaagttacg ggagtggttc cctgaagaag
     3121 agattctatt cagtatttta agtaattttg ctacagaatc attggcttct gcctgttgtg
     3181 aaatcccatt cgaacgctta gggagaaaca aagaaatcgg tgaacaaatt gccaaaaaaa
     3241 tccaacaggc aggggaatat gcgaagcttg acccttaccg tgcagcaacg cacaacaaag
     3301 ggatcatgaa tggtatagaa gctgtagtgg cagctacagg aaatgataca cgtgcggtta
     3361 gcgcttcgat tcacgcctac gcggcaagaa atggattata ccaaggatta acagactggc
     3421 agatcaaagg ggataaacta gttggaaaac taacggtgcc tttggctgtt gccacagtag
     3481 gcggagcttc caatatcctg ccaaaagcaa aagcatcttt agctatgtta gatattgatt
     3541 cagccaagga actagcacaa gtaattgctg cagtagggtt agctcaaaac ctggctgctt
     3601 tgcgtgcatt agttacagaa ggaatccaaa aaggccacat gggacttcaa gctcgttcat
     3661 tagcgatctc aattggggct attggtgagg aaatcgagca agttgcgaaa aaattacgtg
     3721 aagcagaaaa gatgaaccag cagacagcta tacaaatatt ggaaaaaatc cgggaaaaat
     3781 aaaaaagcaa tatctttaga agaataaagc aagatttaca gttattttat ttactgcaaa
     3841 tcttgctttt ttctctatca gaaaatatgt ttattattag aattttttat agttaaggtt
     3901 aggcatctta agaaatcagg caactccaag atatcttcag gtagaagaga agtcgcctaa
     3961 cattgtagga tcataggtga tttgttggac aggtcaagct gatatttgag cctgatgtta
     4021 gaaggaattt ctttttactt gctgtagaaa ttattatatg actaaaaagg ctgggatttt
     4081 aaatcccagc cttttatgac aaatagaatt tttatcgatc agtaatcgct gtcgctgcaa
     4141 attgttcgat caaaggaatc aaagtcaatt tgactttgta tttccgttcg agtgcttttt
     4201 cgataacgaa ctcattttca gtagtcatag aaatagttgc taatttacga ggttcatcag
     4261 catttttttc attaataact acttcatgtt cattttgttc tgtgtaaaat ttaggatact
     4321 cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc attcgtatca gttgagcgtg
     4381 tattttcagt tgtgtccgct tttggttttt ccgttttttc ttctggcaca ctgactgttt
     4441 cttctgtaac tggtgtcgca acagcttgcg cagttggttc atcgtttttc aatcgttgat
     4501 cattccagta agacacgatt tcaataagaa gattgtcacg gaatcgttcg aaaatcccgt
     4561 tttctcctga acgaattctc tttaatacag tattgaagtt gtcatcgatt ggcacgttga
     4621 tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt taagatttga cggtttttgg
     4681 gatcaagcat gacttgtaac ggcaaaccat agcgtcgcaa gctctcttct tctagagaac
     4741 ggaataattg tcctcttaca ccatccaatg aaggacggaa ttgatttaat aatttttgtt
     4801 tctgtttttt cttcatatgt tctctccttc gctcataccc tttcgattat aagaaaaaaa
     4861 cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat acgcaaatta caacagaaaa
     4921 atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa tggaacggat tatgtatata
     4981 tacataatcc gttccattct ttctattttt tcggtatcaa ggcaaaaaca ggtagttcag
     5041 gtgaataaat gacttcatgt tcgtgccagg gtttgaccat tagcgaatga tcgaaccgtt
     5101 ttttcagttc ttcgatgatt tgatcatgtg tccagtttct tgcaggaaaa tgttggattt
     5161 tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt ctgggtaact tcttccgaga
     5221 gagaattcaa gcgttcgatt ggatccataa aatcaccttt cgacttaaaa ttgggtagta
     5281 tctgggtctt ttgctttgcc gactacacca tctaactgat cgatttgttt catttcttct
     5341 tctgttaatt caaaatcaaa taattcagta ttttctttga ttctgcttgg tgtcactgac
     5401 ttaggaagag gaaggaattc gtgttgcagc gaccaacgca aagcaacttg tgcaatcgtc
     5461 ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat cgaagatttt accagtgcct
     5521 aatggactat atgcttcgag gatcatatca tgtttttttg catactcaac tacagttggc
     5581 tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg gcataatttt tgctgttttt
     5641 gctagtgtat ctagatggtg gggtaagaaa ttgctcacac cgattgcttt gattttacct
     5701 gcttcataca gttcttcaaa tgctcgccat gtatctgcat ttgcttgttc ccaattatca
     5761 cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat ctaaataatc tgtttggagc
     5821 ttcttcaatg attcttcaaa agaagacatc acaagttcat agctgtggtt tgcattccat
     5881 aattttgttg tcaagaatat ctcttctcta ggaattccac tttccttaat cgcttggccg
     5941 acactttctt cattcttgta tccttgggca gtatcgatat ggcgataacc tgcagccagc
     6001 gcttctttaa cagaggaaac agcgacatcc ccatcaggtg tttgccatgt gccgaatcct
     6061 actaccggga tttcgtaacc atttaataga cgataagtgc ttgttaatga attcatactt
     6121 tcagctcctt ttttattcgt tcaactataa gtgtagcgca tttttcgaaa aaaagaatcc
     6181 attttgtttc tactcttgaa atttttgttt aaaagaaaat gattttgatt ctggtatttt
     6241 tgattttttt tccgtaaact aagacagaag ggaatgagaa aatggatatc tatttaaaaa
     6301 aagcaattct gcatattatt gacagagaaa ctggttctcc agtcttttca caaaaagagc
     6361 tggatctgac aaaagaatat attcgtgatt ttcttcaaaa gaagatccaa aaaatctctt
     6421 ctgctcaaac aaaaactgga caactagcag aagaaagcat gatcgttgaa tcacttaaaa
     6481 tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt tcagcggtgg ttcgatatct
     6541 accaagaaag tgaagaagca cctagtgcag atgtatttgt tgttttgtat gaattagata
     6601 cagtgatgta cttatcttta ttgaaagtca attatcgtga ggcttacact cactttgttg
     6661 aagcagacga agcagggatc gacaaccgct tgattttgaa tcgagcaatt ctaggggcga
     6721 aaacacaaaa agcagatgaa gcattcgctg tgaatttgac tgattggacc tacgaattaa
     6781 ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta tttttctgga agagtgatcg
     6841 aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt gatccaaaaa gtagccaagc
     6901 atcttggaaa aaaatttgag acagaagaat ttgatatcat ggctgatttg aaagaagctg
     6961 tctatgatac gatagaagaa aaaggtcggt tggatcatga gatgattgcg gaaaaagtct
     7021 tcaaggaaaa catcacggca aaaatggctt ttaaagaaga agtacaagag caaggatttg
     7081 tcccagaagc acctcctgta aaagaagtac aagaaatttc tgagaaaaaa tttggaaaac
     7141 aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc agtagatgta tatcgcaacc
     7201 cagatttgat tgaatttgtg aataatccgg atggtacgat atccgtgatg atcaaaaatg
     7261 tggatgaagt attgaatcgt ttataaaaat gactagaaca ttaggtgttc aagtaccagt
     7321 gatcagttaa atgaattgtg tcaagagaag gatcggaaaa gacgaatata acaaaaaaag
     7381 agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7441 aagcagaagc aactccttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
     7501 atctaataaa attttaagac catttactaa ttatcgattt tttctgccgc atttttaggc
     7561 acttcagaag cctttatttc ttcccagctt aatacacctt tacgaggatt gactttgagt
     7621 ttaagatacg cattcatctt taatggatgt tctctttctt cgcgaagcgt cacctgtttt
     7681 ttttcaccat ctttattata agccgcttgc ttgtaggtat aagtcgtgaa tttctctccg
     7741 ttatctgctt gggtagctga catttcgcca ttcgtcgtga tttttgtata ataagcttcg
     7801 ccgccgtaaa aataatgata agctgcaaaa cttccgccga gaaatatgcc taaaacgaca
     7861 agtacgccaa ttaatttttt catgtacttc acctctttcc ttattattaa cgaaatagaa
     7921 gatttcgtct attgaaagtt gttggcaaca gctgaatttt aaacttttcc tcattttgtt
     7981 cttattgctt caaaagaaag ttaaggacta tcttgaagtt tttctcaaaa aacacatttt
     8041 tttatttata aaaggtactt ttttcgcgta taagacaagc gagtgctctt agttgacttt
     8101 gaccaatatt gacgtataat aagcttagtt cgaatcttac aaaaagtaag tcggacgtat
     8161 tctttttgat ttagtcaaaa aaagactata ataaaaaggt gagagctgaa atttttcagt
     8221 gatctatatt atttaggagg tttgtaaatt atggcaaaag aacattatga cagaagtaaa
     8281 ccacacgtta acattggtac gattggacac gttgaccatg ggaaaactac tttaactgct
     8341 gcgatcacta cagtattagg caaaaaaggc ctagcaaatc ctcaagacta tgctagtatt
     8401 gatgcagctc cagaagaacg tgaacgtggg attacgatca ataccgcaca cgtagaatat
     8461 gaaacagaaa aacgccacta cgcgcatatt gatgcaccag gacacgcgga ctatgtgaaa
     8521 aacatgatta caggggcagc acaaatggat ggtgcaatct tagttgtttc tgcaactgac
     8581 ggtccgatgc ctcaaacacg tgaacacatt ttattgtcac gccaagttgg tgtaaaatac
     8641 ctgattgttt tcttgaacaa agttgattta gtcgatgatg aagaattgat cgatttggta
     8701 gaaatggaag ttcgcgagtt attgagcgaa tatggtttcc caggcgatga cactcctgtg
     8761 atcaaaggtt ccgcattaaa agcattgcaa ggcgatccag atgctgaagc tgctattatg
     8821 gaattgatgg atacagtaga tgaatatatc ccaacaccag aacgtgatac agataaacca
     8881 ttactattgc cagtggaaga cgtcttctca attacaggtc gaggaactgt tgcctcaggt
     8941 cgtattgatc gtggtgctgt tcgtgtcggt gatgaggtag agatcgtagg gatcaaacct
     9001 gaaacacaaa aagcagttgt aacaggtgta gaaatgttcc gtaaaacgtt agattacggg
     9061 gaagctgggg ataacgtagg cgtgttgtta cgggggatcc aacgtgacga tatcgaacgt
     9121 ggacaagtgc ttgctaaacc aggttccatt actccacata caaaattcaa agcagaagtg
     9181 tacgtgttga caaaagaaga aggtggacgt catactccat tcttcaacaa ctatcgtcca
     9241 caattctact tccgcacaac tgatgttaca ggaacaatca cattgccaga agatacagaa
     9301 atggtcatgc ctggtgacaa cgtgacgatt gatgttgact tgatccatcc aatcgctgtt
     9361 gaaaacggaa cgacattctc aattcgtgaa ggcggacgga cagtgggttc aggtatcgtt
     9421 acagagatcg aagcataaga aacgcgactc gaatattttg gtaatgaaaa atgtttaaaa
     9481 taaaagagcg gtccaaacag gatttctgtt tggaccgctc tttgttcttc aattgtataa
     9541 gaagagattg tagaagcatg aactttcgtt ttacttcagt ctcttttatt catcatgaga
     9601 gtgttcatct atcggtatgc agtttttaat actttcttcg tatccatttt ttcaaaccac
     9661 ttattctacc ttgtgtctgc tcaactggcg cattatcaat aatcagcaaa gcaatcataa
     9721 catatgcaat ccaactatct ctagaatata gagtataacg tgagacccag tttgtagcgt
     9781 acttgctttt atattctctt aatccatgga aagaataaaa atgagaacca aactcgtaaa
     9841 ccaagtatgc aatcctttcc tgtaaaaaac tctttctgga agttccgaca tttgaaagag
     9901 gggccatccc taaattgaac cattggatat gttcttcttt catataggca aacaaatgca
     9961 agaacaagaa atccatactg ccagaaggtg cttttttagg atcatatcgc atcagatcaa
    10021 tcgttcctac tttattgttc gtgtaagtag gcatgatcgt tgcaaaagcg acaatttctt
    10081 cttgggtatt tttgacaact gcaataggcg ctctttgtag ataatcttca gagaaaaagc
    10141 ctaaagaaaa ccctttttcc tttcgtgaac ccaaccaatt atcagagata tttttgaaag
    10201 tactcatttg ctcagtagaa aatggtggtt gtaagacatc aaatgtaaat ccttctcgtt
    10261 cgatccgatt caagacagca cgagtgccct tcattttttt ccctgaagtt gtaaaactat
    10321 tcaggtctac ataagcttct tcccccatct taatgaaatc gtagccgaat tcatgcaaaa
    10381 tcataacaat ctcttcgctt gtttcgtaga aaactggtaa atagcatagt cgatctgttt
    10441 cttcgataaa agcttcgatt gcttctggaa aatcttcttt cttaccagaa ggatcaccca
    10501 ttacaataca tttattattg aagcaagcga attggagaag aacagttggt tcaccgtctt
    10561 tttcataggc aaacatccgc ttatctttta agaagatcaa ttgactatcg gagtttcctc
    10621 cataagttgt aaggatcttc agggcctttt cttcattaaa ggcttcgcca atctgtttct
    10681 tttctccttg aaggaaatga acaaatagaa cgatcatgaa actaacagcg atgatggcca
    10741 acaatcccga aaaccaaatc ttttcagaag gaaataagaa aaaggaaata aaatggtgac
    10801 gccgatgagg gaaatccggc agattgtata ccccaataac gatgtaaagc aaggacagtg
    10861 caccaataat gattccatcg atcgtacgcc actcccatga atagatcaat tgttcccgaa
    10921 ataattcgtt tttagaagca agaatgatca gaagcagaat agacaagaaa accaccggtg
    10981 tgaaactaaa atcacttaat aaaacataaa gtaaagctag aacaatcaag aagattgtag
    11041 gcaaatatgc tcgtttcaca cgtgctgcga tccctctgcc cataatgatt aaaagaaatc
    11101 ccaatagtat actaggaaat tggataataa aatgaaattt caatgggttc aaactatgaa
    11161 gccagcgaaa ctctgtaaaa gcttgcggga tagtggcaga aagtactagc atgattccag
    11221 aaaagtataa taagacaacc acgattttat gcgcaatttc tgttgctaat tgtttaggga
    11281 taccagaata acgctgatca aaagtggaac cgatattttt gaagaaaaag acgattccaa
    11341 tcaaaaacgg aatgatgtag tagaacaggc gatagagcaa gatccacaca acgactattt
    11401 ctcttggcac accaatagcg gacaacccga taatcatcat tacatcgaaa ctcccaattt
    11461 ctcctggaat catagacacg attcctatca ctgaagcagc aatgaataac ggaattgttt
    11521 gccacagatc aatccggatt tccattaaat aaccaacaga aaggaaacta aacaggacac
    11581 cggtccattc caacaatgaa actagcaata gctgtccttg agttttaagt gaggtaccac
    11641 caaggaatcc tttctttttg aaagtagtaa ataaaaagac gacaggaaaa tatagaccgc
    11701 cgccgatcaa ccatatccaa tattgcttca aaaaaggagc aactggcgtg aagactacaa
    11761 gtaaaaaaga aagcagactg taaatagaaa gtccagccat caaaaacaag aaaactttcg
    11821 ataatgcttg gatcacgttt ctgctctctt tcttttgtcc atatagttca gagcgaagcc
    11881 ccacactaac aaacccgccg aaaccggcaa tattgttgat tgtattgatc agccagcttg
    11941 tctcaaacaa gtaacgagga ttctgctttt gttcaagcat ccgattcaaa ataatatcat
    12001 aaccaatcat aggtaacaca gagacaagac caatcaacag cataagtccg cttttccata
    12061 caggtatagt ggcaaacgtt tctcctaatt gctggataga tagagtttta ccgatggaca
    12121 tcaattcacc gacaataatg acaatgacag aaatcaaaaa gattgttttg aacagtccta
    12181 ggtggttttt cagccattgt atggtatttt ttaacaaata aatcaccttc tattcctaat
    12241 agttatgttt cagaaagaaa ttgaagaact tctcgattga agcgttcagg atcttttcta
    12301 gctaattcat gtccttgttc tttcacaagc acaaaggaag cttttggtat agttttagct
    12361 atatacaaag aatgttttag cttgattaca tctttttttc ccacgatgat caaagtgggg
    12421 gagttgattt tttttaaatc attttctgta agcccgatgt catgcagcaa aagtttgata
    12481 actaaaagat ttttgcgaag acttgggcga aataaagaga gtaaccatac ccatgcataa
    12541 tgaatattac tgatgactct ggcagaaaaa cgaacgcctt taacaagcgt gtttccagaa
    12601 tttaaaatca atcgatgaac ttttttaggg aagctgctgg caaataccag tgctagattt
    12661 gctccatcac taaatccaag aaaatctgcc tgatcgattt tttccagcaa catgatcgta
    12721 ttcagatcct cggccattaa ctgaaaattc aacatagatg cttcgtttgt cgaacgacca
    12781 tgtcctcggc tatctactag atacacggta tagtagcgtt cgagtacagg gacctgttct
    12841 gaaaaaaagc ggccgctgcc gtcattgcca tgcagtaaaa agagagggaa gccttgaccg
    12901 ctcttttcat agtaaatttc gctcccatct ggcatttttg catatttctt ttcttttttc
    12961 acataatcac ctactgtgta aagtataacg attatatcag tcattgggca attgttttta
    13021 ctgaaaaaca acgatcattt tcgtaagatg acaaaaaata caggaaaatt cattttttct
    13081 taaggcgtac acagaaatca aggaagaaat agccaagaag ataaagaaat agtaagataa
    13141 aaaaagatgt caaaataact aggtgatgaa atgaagcgaa aacgattatc tattatccgt
    13201 attctttttt acatgatcat tctgacggtt attggtgcgg tgttgttttt aggaggaaga
    13261 acttatcgtt cggtaaaacg agtggaaagc tatcaaatag aaataacaac agctgcaaaa
    13321 aattatcata tggaaaacta cgagaatttg atcaaggcga taatattgac cgaatcaaaa
    13381 ggaaaaggga cagatctcat gcggagctcg gaaagtctgc atggagaagc tgagatcatt
    13441 gataatccag ctgacagtat tgatcaaggt gtcaaatatt tggcggaaat gatagtagaa
    13501 gcaaagaaac aaggatgcga tttatggaca gctgtccaag catataattt tgggaaagat
    13561 tatattgcct atgtaaaaaa tcatggcgga gaaaacacag tagaattagc agaagcgtat
    13621 tctaaagatg tgctgagccc acttttagga aatgaggaag ggaaacaata ccgatattgg
    13681 cggatccagt ccttgcttta caatggcggc tatctctatc ataatggagg taacatgttt
    13741 tatcctgata ttgtcaaaat gaatgaaaaa ataatagaga cctatgaaaa agtctttaag
    13801 taaaatagca ggaaatgttc aatcatgaat aaaattaaat gaaagaagga gtgcgcaaat
    13861 agtggaaaaa aggcaaaaaa tggaaaatca agaagtgggg tacacaacca aagactggat
    13921 gttacggttt gtgaagggaa tgtttatcgg ttcaggattt atcttgccag gagtcagcgg
    13981 tggtgcatta gcagctgtct ttggcatgta tgagcggctg atttcttttt tagcccacct
    14041 tacaaaaaat ttcaaagaaa atgttttgtt ttttctgcct gttggtctag gcggagtgac
    14101 aggcatcttt ctactttctt ttttagtcag ttttttatta ggtgcgtatg aaacaacgat
    14161 attatggttt tttgtcggat gtatcgctgg aactgttcct gcgttatgga aagaagcagg
    14221 gaaaaaagga agagatactt ctgatgtgat catcatggtt gttacttttg ttctggctta
    14281 tttgttttta ttatacggcg ctcaattatt tgacgggcaa gtcgatcaga attttggaac
    14341 atggctgatt gccggcggat tgattggttt gggtatgatc gtcccaggat tgagcccttc
    14401 taattttttg gtgtatatgg gattgtataa agcgatggct gacggattta aagaagtacg
    14461 acttgaagtg attgtgccga tcggaattgg tgggattctt tgtgttcttg gattgtcaaa
    14521 aatcatggat tacattttct caaaggcata tagcaagcta ttccatttca ttttaggtgt
    14581 agtttttgct tcaacgatta tgatcattcc taccgattac gcgggattgg gagttgctgg
    14641 ggtactagct tgtcctgttc tttttatagc cggtacagca ctaggttggt ggatgagccg
    14701 tttggaggaa caatataagt aaagatagat tgataataaa aacagcacac gggtgattca
    14761 aacaaaaagg ttggcatctt ttttgtaaaa tcattcgtgt gtttttctat aaattttata
    14821 aaaacaagtt gtttttaatc ttggaacacg ctagacttaa acacgtgatt atttttgaaa
    14881 gttggcggaa aatggagaaa aagaaaatga atggacttac acagcaagag gtagttcaga
    14941 aaatggcttc tggccagcaa aatgactacc aggaagatgc atcaaaaagt acaaaacaaa
    15001 tattcagcga taatatattg acgttattca attttctgaa ctttggtatt gggatttgtc
    15061 tgttatttgt aggtgcgtat tccaacatgg cgtatttagc aattattgtc gtcaatatcg
    15121 ttattggaat ttatcaagaa atacatgcaa gaaatcttgt acggaaatta tcgattgttt
    15181 cccaatcaga agtactagtg atccgggacg gtaaagaaca atcgatttct actaaagagc
    15241 tggtattagg agatttggta aaaatcggag caggagaaca gataccagcc gatttacagg
    15301 ttatatctgg acgggcagaa gcaaatgaag ctcttttgac aggcgaatct gatttgattg
    15361 tcaaagaaac tggagctgaa ttgctttctg gaagcttttt gacaagcggt tctgtcatgg
    15421 ctgaagttat ccgagtaggt gcagataatt atgctgtccg cttgactagc gaggcaaaga
    15481 ctcacaaagc tattcgttct gaacttgtag attcgattcg caaagtatcc aaattcacta
    15541 gtttcgtgat tattcctttg gggatcatac tatttttgga agcactgttc atccgcgatg
    15601 cttctatcaa aatttctgtt attgcatctg cagcagcttt gttaggcatg cttccaaagg
    15661 gtttggtatt gctaataagt attgcattaa cgacaggggt aaccaaactg gcaaaaaaac
    15721 gaatccttgt tcaagacatg tattcaattg aaacgctggc ccatgtcgat acattatgct
    15781 tggataaaac agggaccatt actgaaggga aaatgagtat ccagcgggta gaagcactac
    15841 atccgctgta cgagaaagcc atcccagaaa ttatggggac gtatctggca gaaagcagtg
    15901 ataacaacat cactatgcgg gctatgagag attattttga gctttctaat cggtatgatg
    15961 tgacaaacaa agtcgcattt tcttctgatc gcaaatgggg cgcgatggaa tttccagaac
    16021 tgggagtcgt gtacgtagga gcgcctgaac gtttggtgga agaagatcgg cttccggaca
    16081 gtctaaaaac agcccaagcg aatggctatc gcgtacttat gttggggatc gctccaaatc
    16141 agcaattgaa cgaagaaaaa ccagcatttg ttgagccggt tgctctgttt gaaatcgatg
    16201 atccaatccg aaaaaacgct caagaaacac tagcttatct tcatgaggaa ggtgtggatc
    16261 tgaaagtgat ttctggagac aatcctttta ctgtctccaa tatcgctaga cgtgcaggat
    16321 taccgaatta tgatgcgtat gtcgatcttt ctcaaattac tgaagaaatg gaagtgcgag
    16381 aagctgctca tcgctatacg gtttttggac gagtgagtcc gcagcaaaag aaattgctgg
    16441 taaatgaatt gaaagaaagc ggcagaacgg ttgcaatgac aggcgacggt gtgaatgatg
    16501 ttttggcgtt aagagaagca gattgcagca ttgctatggc agaaggtgat ggagcaacac
    16561 gtcagattgc gaatctggta ttgttggact ctgattttac tacattgcca gaagtcctgt
    16621 ttgaaggacg ccgagtggtc aacaatgtga cgaaagtctc aggcatcttt tttatcaaaa
    16681 cgatttattc atttatttta tcgattatat gtgccgttac agcaattgga tttccgttta
    16741 tccctatcca aatcacgctg attgatttag cgattgaagg gtatccttcc ttctttttat
    16801 cttttgaaca agataaaaga aaaatcactt accgttattt gcctacagca cttattaatg
    16861 ctttaccaaa tgccttgctt gtagtagtga atatcattgc ggtgtatcta cttggaaaaa
    16921 cattcgcatg gggaaatgct gaaactacta ctttgatgta ttacttgctg attgggatca
    16981 gttgcatggc agttgtaaaa gcttgttatc ccttcaaccc attgcgcatc ttcctagcag
    17041 taacgacgat cgtagggata tatgtagctg caatgctgtt ccatggaatt ttggaagtaa
    17101 acttagggtt gggagagagc gtaggatact tctttgtatt catgctcgta aatattgttc
    17161 ttcgagtatt gtactggaag gctgatattc aaaatcagtt actgaaaaga gtagcaaatt
    17221 gatgcttaat gagatagaaa tgtgttatac ttgaaaaagc aatgaaatat gatacaatca
    17281 aacatgtata attgtgtcct ttaaaatcgc actgatttca gttagtgcga ttttttaccg
    17341 gttattctcc tgaaatatct tctgttaaac ccaaaagata tcaatagagc agggggtgaa
    17401 aaaatggctc aacggattcc tcaagaggtt atcgaaacag tacgcagtca aacgaatatc
    17461 gttgaggtga ttggacaata tgtccagttg aaaaagtctg gtaaaaatta cttggggctg
    17521 tgtccttttc atgaagaacg tacaccgtct ttttctgttg cagaagacaa gcagattttt
    17581 cattgctttg gttgtggtaa aggcggaaac gtgtttactt ttcttcaaga actagaaggt
    17641 ctaagttttc cagaagcagt ggtaaaagtg gcagacttcg aacagatacc gttagaagat
    17701 caatggcgtc agcgtactat agctgtccaa aatcctgaat ctgctacggg gaaactgatt
    17761 gctgctcatg aaaaagcagc ggaaatctat catcacatgc tattgcatac aaaagctgga
    17821 cagccagctt tagaatattt gcaaagtcgt gggttgtctt tagaattaat tgaagaattc
    17881 aacataggtt ttgcacctaa tgagcgttct tttcttcagc aagtattcaa gaatgaatca
    17941 atttcaaatg aattgttaga acgctctgga ttgtttgtca cgcatgatga tggccgtatg
    18001 tctgatcgtt tttatcaaag agtgatgttt ccacttcgta attctcaagg ccagacgatc
    18061 ggtttttcag gacgctggct tgcacctgaa aaagggcagg aaaaagatca gcccaaatac
    18121 ttgaatagtc cagaaacaga actgttcaat aaaagacaag ttttatttaa tcttgataaa
    18181 gcaagaagcg atatccggaa aaacggagaa attttgttgt tcgaagggtt tatggatgtg
    18241 attgctgcat ggcaggctgg tatcaaaact ggattagctt ctatggggac aagtctaaca
    18301 gctcaacaga tccaacaaat ggaaaagctg acgaaagaat tggtcttttg ctatgacgga
    18361 gacaacgcag gttttgaagc aaccaatcga gggattgaat tgcttcggca aaacagtcga
    18421 ttagcacttt ctgtcgtagt cgttccagaa aagcttgatc ctgatgaata tctccgtaag
    18481 tatggagaag catctttcca agaattagtg gctcatgggc gcgaaacagt ctttactttt
    18541 aaatcacgtt atttaaaaca agggaaaaat ttagataatg aaaaagacaa gatcgaatac
    18601 ctcgaagaat tactagttga acttagccga gttgtttcac caatcgaaca agatatgtat
    18661 ctatctcaac tgtcaacaga gtttcagatc ggcagagaga cgatacaaaa acaactgcgc
    18721 gatcttcgaa ggacaaatca gcaaaatcgt caagaacctc aggaaagtca acacactcag
    18781 gttacgagac aagaaactgc acggaaaaag agacctttga cgcaagtaga aaaggcagag
    18841 cagattctat tatatcgtgc atttaaagaa gtttctgtcc gtaatatttt aaaagaacga
    18901 gaatttcagt ttattcatga tgcatatgca gaagtttatt ttttattcga tgcatatgtc
    18961 tcacaacatt atgaattcaa ccttgcagag tttttagact ttttgcaaga tgaaaattta
    19021 aggcggttgg tggtggaaat cgaatattta ccggtggcag aagaaagttc gccacgagaa
    19081 atccaagact tgctagatgt gatcagcaaa tctggcttag ctgatgaaat aacattaaaa
    19141 aaacaaatgc agcaagaagc cagacgaact ggaaacaaac agcttgagct tgaattgacg
    19201 attgaaatca tcaatttaac gaaacagtta aaacaagcca aatagctact caaggggggc
    19261 cttcttttat ggcaaatgga acagataaaa aaacgtatga agcagcagta gcagctttca
    19321 tcaaagaaaa caaacccaaa cgacaagttg tatatgatga tttatccaat cagcttgcta
    19381 caccattcac tttgaatgca gatgaaatgg acaaattgat ccaaaagatt gaagacgcag
    19441 gtatcagtgt ggtggatgaa aatggtgatc catcaattca cagcttgaag agtgctgaga
    19501 aaaaagctga acaagcaaaa acagaagatc tttctgcgcc aaccggtgta aaaatcaatg
    19561 acccggttcg tatgtatctg aaagaaattg gccgtgtctc ccttttaact gcggaagaag
    19621 aagtagcgtt agcattgaaa atcgaagaag gcgaccaaga agcgaaacag cgtctggcag
    19681 aagcaaacct tcgtttagta gtaagtatcg ctaaacgtta tgtaggacga ggcatgcagt
    19741 tccttgactt gatccaagaa ggaaacatgg gactgatgaa ggcagttgaa aaatttgact
    19801 accgcaaagg atttaagttt tcaacctatg ctacatggtg gattcgtcaa gccatcactc
    19861 gagcaatcgc tgaccaagca cgtacgatcc gtattccagt tcatatggtt gaaacaatta
    19921 ataaattgat acgtatccaa cgtcaattgc tgcaagacct tggaagagaa cctacgccag
    19981 aagaaatcgg tgcggaaatg gatcttccaa cagaaaaagt ccgagaaatc ttgaaaatcg
    20041 cgcaagaacc tgtttccctt gaaacgccaa tcggtgaaga agatgactct cacttaggtg
    20101 atttcataga agaccaggaa gcaacaagtc cagcagaaca cgcagcttat gagttactaa
    20161 aagaacaatt ggaagatgta ctagatacat tgactgatcg ggaagagaat gtccttcgct
    20221 tacgttttgg tttagatgat ggccggacga gaacattgga agaagtaggg aaagtgtttg
    20281 gtgttactcg ggaacgtatt cgtcagatcg aggctaaagc attaagaaaa ttgcgccatc
    20341 cttctcgttc aaaacaatta aaagatttct tagaataagc gtggactatt gaccagaaga
    20401 gaaatcagta aaactgtttg gtttatttta aaagaaaaa
//
LOCUS       DACDCJ010000052        20385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDCJ010000052 DACDCJ010000000
VERSION     DACDCJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.052"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            40..768
                     /locus_tag="IV038_001846"
     CDS             40..768
                     /locus_tag="IV038_001846"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP8342051.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            768..1022
                     /gene="purS"
                     /locus_tag="IV038_001847"
     CDS             768..1022
                     /gene="purS"
                     /locus_tag="IV038_001847"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP8342052.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1024..1695
                     /gene="purQ"
                     /locus_tag="IV038_001848"
     CDS             1024..1695
                     /gene="purQ"
                     /locus_tag="IV038_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP8342053.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1696..3918
                     /gene="purL"
                     /locus_tag="IV038_001849"
     CDS             1696..3918
                     /gene="purL"
                     /locus_tag="IV038_001849"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP8342054.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3903..5342
                     /locus_tag="IV038_001850"
     CDS             3903..5342
                     /locus_tag="IV038_001850"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP8342055.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5374..6417
                     /locus_tag="IV038_001851"
     CDS             5374..6417
                     /locus_tag="IV038_001851"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP8342056.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            6414..6992
                     /gene="purN"
                     /locus_tag="IV038_001852"
     CDS             6414..6992
                     /gene="purN"
                     /locus_tag="IV038_001852"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP8342057.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6989..8524
                     /gene="purH"
                     /locus_tag="IV038_001853"
     CDS             6989..8524
                     /gene="purH"
                     /locus_tag="IV038_001853"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP8342058.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8540..9814
                     /gene="purD"
                     /locus_tag="IV038_001854"
     CDS             8540..9814
                     /gene="purD"
                     /locus_tag="IV038_001854"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP8342059.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9936..10850
                     /locus_tag="IV038_001855"
     CDS             9936..10850
                     /locus_tag="IV038_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP8342060.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10983..12548)
                     /locus_tag="IV038_001856"
     CDS             complement(10983..12548)
                     /locus_tag="IV038_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8342061.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12541..13992)
                     /locus_tag="IV038_001857"
     CDS             complement(12541..13992)
                     /locus_tag="IV038_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8342062.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14006..15022)
                     /locus_tag="IV038_001858"
     CDS             complement(14006..15022)
                     /locus_tag="IV038_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8342063.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15242..16330
                     /locus_tag="IV038_001859"
     CDS             15242..16330
                     /locus_tag="IV038_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8342064.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16384..17910
                     /locus_tag="IV038_001860"
     CDS             16384..17910
                     /locus_tag="IV038_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP8342065.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17912..19117
                     /locus_tag="IV038_001861"
     CDS             17912..19117
                     /locus_tag="IV038_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8342066.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19374..20351
                     /gene="guaC"
                     /locus_tag="IV038_001862"
     CDS             19374..20351
                     /gene="guaC"
                     /locus_tag="IV038_001862"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP8342067.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6526 a         3459 c         4676 g         5724 t
ORIGIN      
        1 tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa agaacttttg
       61 tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat ttggatagaa
      121 tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag tggaaaagga
      181 gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag aggaataaaa
      241 aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt acaaattatc
      301 cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt
      361 cccgaaggta aagaattcat caaaccaatc attgaattct actataagga tgaccagttg
      421 gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa gccggaagaa
      481 atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga attatttgct
      541 aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa tggacaaatt
      601 ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg tacgaatgaa
      661 catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa
      721 gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg tatttcgtaa
      781 aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca gtaaaaaaag
      841 cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa tactttgaga
      901 tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc
      961 tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg gaggaagtat
     1021 aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg
     1081 gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga gtcctcttta
     1141 gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta tttgagatgc
     1201 ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg
     1261 aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg acttttgcca
     1321 ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc attgaaagtc
     1381 aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta tcttccagtt
     1441 gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat
     1501 aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa cattgcaggg
     1561 attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg tgcagtagaa
     1621 gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa aaacttcgga
     1681 aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat caagaaaaaa
     1741 agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga agaagatatc
     1801 ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg gagcgaacat
     1861 tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg acctcatgtg
     1921 ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact agcagttgtc
     1981 ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg agctgcgaca
     2041 ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat tgcgttgcta
     2101 gattcattga ggttcggcga attagataat ccaagaacga aatatttact agaagaagta
     2161 gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg cggcgaaatt
     2221 gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt
     2281 gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat catgtatgta
     2341 ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga agaatttaat
     2401 caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat ggaaaagctg
     2461 ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat aggtatacag
     2521 gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa agctggctca
     2581 ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac accttatgaa
     2641 atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg agaggaagaa
     2701 aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg taaagtaaca
     2761 gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct gccagtcgat
     2821 gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc tcgaatcgta
     2881 gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca agtattgctt
     2941 gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac ttatgattca
     3001 caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga
     3061 gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct gtatttggat
     3121 ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat tgctagcggt
     3181 ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa accagaagtt
     3241 ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac gttaggaact
     3301 cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc aatctatcca
     3361 acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac tactcaggaa
     3421 ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga ttttgatggc
     3481 agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta
     3541 tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg attgattgaa
     3601 agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc agctttcaaa
     3661 catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt tgcagaaact
     3721 caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga agaaatgatg
     3781 ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga aatttcagca
     3841 actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga ggatgcaatc
     3901 gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt gtttggggtc
     3961 atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat cgaggacaag
     4021 aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga gatttggggc
     4081 tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga gaagctgcaa
     4141 tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt cagccttttc
     4201 tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg atcaatgctc
     4261 gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac tccgatacgg
     4321 agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa
     4381 gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa acggcaatga
     4441 ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg aaaaacggag
     4501 catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt tttataagag
     4561 atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt gattgcttta
     4621 cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc agaccagatt
     4681 caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg aatttagcga
     4741 aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct ttgtcagccg
     4801 caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta aaaaatcaat
     4861 acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt gttcgaatga
     4921 aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc gatgattcaa
     4981 ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca ggggcaaaag
     5041 aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac gggattgata
     5101 ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc aaagaatgta
     5161 tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg atcggtctgg
     5221 aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat tatccgacgc
     5281 cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta tcgttctttt
     5341 aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc aaaagctggc
     5401 gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt caaaaaaacg
     5461 gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga tttatcggtc
     5521 tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac gaaattagca
     5581 gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc gatgtgtgtc
     5641 aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt agcaacgggg
     5701 aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa
     5761 ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat
     5821 gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt attgacagga
     5881 gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat tcattcaaat
     5941 gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt atcatcaaaa
     6001 ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa aatctatgtt
     6061 tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca tatcactggt
     6121 ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa
     6181 cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg tgaaattcct
     6241 gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc agtcgaagaa
     6301 tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc
     6361 gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga acgatgagaa
     6421 tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat tatctatcga
     6481 aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa gcatatgttt
     6541 tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa gaatttgatt
     6601 caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag atcgatttga
     6661 tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat tatgataaaa
     6721 gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat ggcatacgtg
     6781 atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg
     6841 tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag gacacgttcg
     6901 attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa gtgattagtg
     6961 aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg tatcagataa
     7021 aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa tcatttcaac
     7081 aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga tcgaagaagt
     7141 gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta aaatccatgg
     7201 tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa cggaaaatat
     7261 ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga ttatgaaacc
     7321 agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta gtatgctccg
     7381 tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg actatgaaga
     7441 agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac aaaaactagc
     7501 ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat acttaacgga
     7561 ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa aacaaacgct
     7621 tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc ttctggaaaa
     7681 atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata ataatatccg
     7741 agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt
     7801 gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag cgtgggaagc
     7861 agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga atcgagaagt
     7921 agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca ttgccccttc
     7981 ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat tactgacagt
     8041 agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct taggtggact
     8101 tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa
     8161 acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag ctgtcaaaca
     8221 tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa tcggagccgg
     8281 gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga
     8341 aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg aatatgcagc
     8401 caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc aagattctat
     8461 tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc attttagaca
     8521 ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc
     8581 aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa
     8641 tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag atcattcagc
     8701 gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc ctgaagttcc
     8761 tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct tcggtccgac
     8821 aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa tggatactta
     8881 tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag cagcgtatat
     8941 caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt
     9001 agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt tgaaccagca
     9061 tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag gagaagaatt
     9121 ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt cgcaagatca
     9181 taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg cttattcacc
     9241 agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt taaaaccagc
     9301 ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg cgggtttgat
     9361 tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg atcctgaaac
     9421 gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg atttattaga
     9481 gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg ttgtggttgc
     9541 agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt ttctggaaga
     9601 agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag ttggagcagg
     9661 aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac agaaaaaaat
     9721 ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag atattggaga
     9781 aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat
     9841 acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag agtgtggtac
     9901 acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa ttaggtactt
     9961 ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat gcggaagatc
    10021 cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac catgcagata
    10081 tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa acatcaatca
    10141 aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt
    10201 tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg aaaatggatt
    10261 atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag gaagtagctg
    10321 aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa
    10381 aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta gctaatcagc
    10441 tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc aatatgacag
    10501 atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg catgatatga
    10561 cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt atcgggaacg
    10621 aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat caaacgacac
    10681 caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa gtgatcgccg
    10741 gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc
    10801 gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa agattgtcat
    10861 aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct gttttcagct
    10921 tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac ggatttcgtt
    10981 tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg agaaatccct
    11041 tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat ttcaaaagca
    11101 atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc gattctttgt
    11161 tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt actgaaatga
    11221 gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg atcggtaaaa
    11281 ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt ccttgaaggc
    11341 tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag
    11401 tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg
    11461 attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa taaacgatat
    11521 aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta cttagatact
    11581 tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac agaatcaact
    11641 ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt aagggaactg
    11701 gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct gattctttct
    11761 gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa ttcatggacc
    11821 aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga atcaactatt
    11881 aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt ctccctaaat
    11941 aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact gacttggttt
    12001 gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga ttcagcaagt
    12061 ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa atgatttttt
    12121 ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa atctaaagca
    12181 gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt aagcagataa
    12241 tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa tccactaata
    12301 aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc
    12361 atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat aattgaagac
    12421 agtgccatct caccatctcc agcttcgccg acataagtga ttggataata agaaccaagg
    12481 acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat gataaaaact
    12541 ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca gtgtgacagt
    12601 tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc cgtaataaag
    12661 aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc
    12721 tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa tcccgttgtc
    12781 tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa taaatccttt
    12841 gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct gcaagatcat
    12901 ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt caagcatatg
    12961 gccgtatctt atttttaaga tgactagata atcattgata tggctaattt ctttttcaag
    13021 aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt
    13081 catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg catccaaact
    13141 gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac tttgtgctaa
    13201 atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg ccattttatt
    13261 catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac gaaagccaag
    13321 gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt cttggacctt
    13381 tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa gaactccaag
    13441 ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa
    13501 gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag tgacagaatc
    13561 tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga tattattgat
    13621 cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat ctaacgtact
    13681 aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc taagttcttc
    13741 taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca agtcccacat
    13801 actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt ggatatttgc
    13861 gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca gtagcaaagg
    13921 gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa
    13981 attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta gctatattct
    14041 gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt cctgtttgaa
    14101 tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca ggtttacact
    14161 cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct tgtccatcaa
    14221 tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc gcagctaaag
    14281 caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat aaatcatttg
    14341 tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca ccgacaattc
    14401 ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt agggttggtt
    14461 tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg gagttctctg
    14521 aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag tgattgctga
    14581 caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa
    14641 gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc cctgctcgct
    14701 ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa atgatcatat
    14761 ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga ttcatgtagc
    14821 catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt gattcagtat
    14881 gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact agtgggtcag
    14941 gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca acaagggcta
    15001 aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag tactatctta
    15061 tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta
    15121 aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc ataaaaaaat
    15181 caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg ggggataaaa
    15241 aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg caagcattct
    15301 attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg taggaatcgc
    15361 tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg tctcggaatt
    15421 agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag aaaatcaagt
    15481 agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg cttctatcga
    15541 tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca aggtgatcgc
    15601 ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt ttgataattt
    15661 tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga aagaaggaaa
    15721 aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg cactaatcaa
    15781 ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt tggtcgtgcc
    15841 atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca
    15901 agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg atgctgtttt
    15961 gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg tggggtatgg
    16021 gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg ctggcgttaa
    16081 atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta tcttagcaaa
    16141 aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg tcaatgatac
    16201 tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata cagtatctgt
    16261 cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag aatccgatct
    16321 gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga gaggataaga
    16381 attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc tggaatccga
    16441 gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa
    16501 aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc atatggctcc
    16561 tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt
    16621 gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta tctttcaata
    16681 aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga ttgggaactg
    16741 acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga cccgaatctt
    16801 ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa agcattatca
    16861 aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga agaagaaagc
    16921 gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc catcattatt
    16981 tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt acgggatgga
    17041 cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat tcgcggaatg
    17101 gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg agaagtttat
    17161 tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat cataaacgac
    17221 cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact gatgggagcg
    17281 ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa gatatccgga
    17341 cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc cattgcaaac
    17401 ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct gatggatatt
    17461 cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt attagataaa
    17521 gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc
    17581 atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt tttatccaag
    17641 tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg gatagatgtt
    17701 ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg caaagctgtc
    17761 tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat ctatacgatg
    17821 aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga agtcttgatg
    17881 aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc ttacggataa
    17941 aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc gctacagcat
    18001 ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg gtattttctg
    18061 gcgtccattg aatataacta accttgttct gcaaaatagc catatattag tactagctgc
    18121 agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt cagtgatggc
    18181 gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac
    18241 agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt tttgggtagc
    18301 ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt ttcgtggact
    18361 gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt tccaaaaaat
    18421 gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct tgacattgat
    18481 tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg
    18541 gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg cggtatttac
    18601 agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt atccgattat
    18661 tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata aaacagtagc
    18721 aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa
    18781 aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag ctttagctgg
    18841 attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat cgatggaact
    18901 ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca ttggaacaat
    18961 catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca tgtcgatttt
    19021 aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt tagccgttgt
    19081 gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt attcagaaaa
    19141 aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac attcattgtt
    19201 tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa ttctcttttt
    19261 gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa aagccttgat
    19321 taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat gtaatgaaag
    19381 tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat agccgttctg
    19441 aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta gtgcctgcca
    19501 acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat ggttatttct
    19561 atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc
    19621 gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc tttatagaga
    19681 agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg catggacatg
    19741 ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa acttttgtga
    19801 ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg
    19861 caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag acaggctttg
    19921 ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct cgcaagccga
    19981 tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc cgttttggtg
    20041 cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa
    20101 aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa ttccaaaaag
    20161 gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga tctttgaaag
    20221 atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct ggaggaagag
    20281 atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt tttaatggag
    20341 acacaatcta aaaaataaat aaaatattag gatgttgtga aaaag
//
LOCUS       DACDCJ010000053        20198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDCJ010000053 DACDCJ010000000
VERSION     DACDCJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.053"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            72..506
                     /locus_tag="IV038_001863"
     CDS             72..506
                     /locus_tag="IV038_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8342068.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(584..1117)
                     /locus_tag="IV038_001864"
     CDS             complement(584..1117)
                     /locus_tag="IV038_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP8342069.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IV038_001865"
     CDS             complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IV038_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP8342070.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IV038_001866"
     CDS             complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IV038_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP8342071.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3286..4668
                     /gene="rlmD"
                     /locus_tag="IV038_001867"
     CDS             3286..4668
                     /gene="rlmD"
                     /locus_tag="IV038_001867"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP8342072.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4842..5420)
                     /locus_tag="IV038_001868"
     CDS             complement(4842..5420)
                     /locus_tag="IV038_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP8342073.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5742..6212
                     /locus_tag="IV038_001869"
     CDS             5742..6212
                     /locus_tag="IV038_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP8342074.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6320..7546)
                     /locus_tag="IV038_001870"
     CDS             complement(6320..7546)
                     /locus_tag="IV038_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP8342075.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8300..8500)
                     /locus_tag="IV038_001871"
     CDS             complement(8300..8500)
                     /locus_tag="IV038_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP8342076.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8634..9242)
                     /locus_tag="IV038_001872"
     CDS             complement(8634..9242)
                     /locus_tag="IV038_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP8342077.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9327..9797)
                     /locus_tag="IV038_001873"
     CDS             complement(9327..9797)
                     /locus_tag="IV038_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP8342078.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9845..10759)
                     /locus_tag="IV038_001874"
     CDS             complement(9845..10759)
                     /locus_tag="IV038_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP8342079.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10905..11708
                     /locus_tag="IV038_001875"
     CDS             10905..11708
                     /locus_tag="IV038_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP8342080.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11886..12833
                     /locus_tag="IV038_001876"
     CDS             11886..12833
                     /locus_tag="IV038_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP8342081.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12901..13194)
                     /locus_tag="IV038_001877"
     CDS             complement(12901..13194)
                     /locus_tag="IV038_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP8342082.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13222..13887)
                     /locus_tag="IV038_001878"
     CDS             complement(13222..13887)
                     /locus_tag="IV038_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP8342083.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14027..14659)
                     /locus_tag="IV038_001879"
     CDS             complement(14027..14659)
                     /locus_tag="IV038_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8342084.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14666..15733)
                     /locus_tag="IV038_001880"
     CDS             complement(14666..15733)
                     /locus_tag="IV038_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8342085.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15730..16461)
                     /locus_tag="IV038_001881"
     CDS             complement(15730..16461)
                     /locus_tag="IV038_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP8342086.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IV038_001882"
     CDS             complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IV038_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP8342087.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IV038_001883"
     CDS             complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IV038_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP8342088.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18624..19793
                     /locus_tag="IV038_001884"
     CDS             18624..19793
                     /locus_tag="IV038_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP8342089.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         6122 a         4028 c         3244 g         6804 t
ORIGIN      
        1 tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag
       61 gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg
      121 ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa
      181 tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc
      241 aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga
      301 ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact
      361 ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt
      421 tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc
      481 atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc
      541 ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt
      601 gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca tctccattat
      661 tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt
      721 aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt
      781 caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca
      841 aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc attgctatta
      901 cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc
      961 gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg
     1021 tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt
     1081 ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat
     1141 tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa
     1201 tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt
     1261 tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag
     1321 ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa
     1381 catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa
     1441 caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa
     1501 acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg
     1561 tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt
     1621 caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg
     1681 gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac
     1741 aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac
     1801 ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca
     1861 tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca
     1921 atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga
     1981 tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag
     2041 gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt
     2101 tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag
     2161 catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca
     2221 ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac
     2281 gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt
     2341 gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc
     2401 ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc
     2461 tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct
     2521 ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct
     2581 atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt
     2641 ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga
     2701 tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca
     2761 ggtttcaaac ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag
     2821 cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct
     2881 tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat
     2941 gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa
     3001 ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt
     3061 ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca
     3121 gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata
     3181 aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat
     3241 tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt
     3301 gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga
     3361 atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta
     3421 ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa
     3481 aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt
     3541 caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa
     3601 gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg
     3661 atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag
     3721 actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca
     3781 gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg
     3841 aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc
     3901 atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa
     3961 aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt
     4021 tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc
     4081 cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct
     4141 cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc
     4201 aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg
     4261 atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa
     4321 gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa
     4381 gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt
     4441 atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa
     4501 taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg
     4561 gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag
     4621 actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt
     4681 gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg
     4741 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
     4801 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
     4861 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
     4921 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
     4981 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
     5041 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
     5101 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
     5161 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
     5221 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
     5281 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
     5341 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
     5401 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
     5461 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
     5521 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
     5581 aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt
     5641 aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga
     5701 gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata
     5761 ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta
     5821 gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct
     5881 ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt
     5941 tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt
     6001 tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag
     6061 caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc
     6121 atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc
     6181 tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta
     6241 acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta
     6301 ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat
     6361 aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg
     6421 gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact
     6481 gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat
     6541 caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat
     6601 ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt
     6661 ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc
     6721 cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta
     6781 catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa
     6841 tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta
     6901 cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag
     6961 aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat
     7021 ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt
     7081 ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa
     7141 ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg
     7201 ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt
     7261 tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc
     7321 ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc
     7381 ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg
     7441 tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa
     7501 cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt
     7561 tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc
     7621 ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa
     7681 attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc
     7741 aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc
     7801 aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc
     7861 taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt
     7921 aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa
     7981 ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac
     8041 gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac
     8101 aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa
     8161 gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt
     8221 agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt
     8281 gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac
     8341 cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt
     8401 gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc
     8461 ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa
     8521 agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt
     8581 tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt
     8641 gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata
     8701 taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct
     8761 agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct
     8821 aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct
     8881 tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt
     8941 atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg
     9001 ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc
     9061 atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct
     9121 gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct
     9181 tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc
     9241 ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag
     9301 agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct
     9361 tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata
     9421 acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac
     9481 aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg
     9541 cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc
     9601 gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt
     9661 aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca
     9721 ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac
     9781 tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta
     9841 tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg
     9901 tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac
     9961 gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga
    10021 aaataattat cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat
    10081 cgattgcttc aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca
    10141 aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga
    10201 tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga
    10261 taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat
    10321 tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc
    10381 caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct
    10441 tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag
    10501 cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg
    10561 ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag
    10621 catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga
    10681 tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag
    10741 gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt
    10801 tttgatattt caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact
    10861 tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat
    10921 taattatcag ttcctttata cgattcaaag acctaaagga ggcgagaaat gtcccatttt
    10981 ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc
    11041 aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc
    11101 ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc
    11161 aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct
    11221 ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag
    11281 ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct
    11341 acaccaatga atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag
    11401 catctatacc actttggata aaagtcctga catttatatt ttctatttat cctagttttc
    11461 tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc
    11521 gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc
    11581 ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca
    11641 ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga
    11701 tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt
    11761 gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag
    11821 caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat
    11881 taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag
    11941 aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac
    12001 aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt
    12061 ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt
    12121 tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg
    12181 agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac
    12241 agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa
    12301 tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag
    12361 acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt
    12421 tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac
    12481 ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa
    12541 aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg
    12601 ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc
    12661 cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt
    12721 tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact
    12781 ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga
    12841 aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt
    12901 ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa
    12961 atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat
    13021 ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc
    13081 atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag
    13141 atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac
    13201 aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc
    13261 tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga
    13321 agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct
    13381 gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc
    13441 taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg
    13501 cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc
    13561 aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc
    13621 ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac
    13681 atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac
    13741 aacagcatga gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc
    13801 cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag
    13861 acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt
    13921 ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa
    13981 taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac
    14041 catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata
    14101 tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct
    14161 gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt
    14221 catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg
    14281 gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg
    14341 tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt
    14401 catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt
    14461 ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc
    14521 gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa
    14581 tgtcttcttg aatagataaa taagaagaga cgcctaagcg caccatttca tggtcatcta
    14641 ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta
    14701 cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc
    14761 gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg
    14821 ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta
    14881 attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca
    14941 agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct
    15001 ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt
    15061 ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga
    15121 tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg
    15181 cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat
    15241 catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc
    15301 catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt
    15361 tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac
    15421 ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct
    15481 tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta
    15541 atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg
    15601 cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg
    15661 aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata
    15721 tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag taataatctt
    15781 caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa
    15841 agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat
    15901 ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat
    15961 attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac
    16021 ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa
    16081 ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat
    16141 ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt
    16201 cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa
    16261 gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg
    16321 atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc
    16381 agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa
    16441 aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca tttcatcata
    16501 cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa
    16561 aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt
    16621 tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt
    16681 agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc
    16741 attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc
    16801 ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc
    16861 atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat
    16921 tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag
    16981 atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt
    17041 tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt
    17101 ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag
    17161 gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat
    17221 gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat
    17281 atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata
    17341 ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc
    17401 aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt
    17461 atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga
    17521 ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc
    17581 attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt
    17641 caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac
    17701 catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa
    17761 taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc
    17821 tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc
    17881 tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc
    17941 ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg
    18001 caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg
    18061 aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat
    18121 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac
    18181 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
    18241 cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat
    18301 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
    18361 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
    18421 aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg
    18481 gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata
    18541 agattaaaat aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa
    18601 gaaaggagac taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt
    18661 catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc
    18721 ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc
    18781 aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaatc gatcaactat
    18841 taagaaaaaa tgattatatt ggctcaattt acgtaaagaa aaataatcgc atcatcatgg
    18901 aaaaaggcta tggctatgcg aataaactca cgcaacaacc aaatgatcct tctctttatt
    18961 atcagattgg ttcggttcaa aaagcattga ctactctttt gattttaaaa caagtcgagc
    19021 gtgggcacct ttctttagat actaagctgg ctcaattcta tccacaaatt gctggcagcc
    19081 agcaaattac tatccgtgac ttgctgtata tgcgctcagg cttgcaccga actgcttctc
    19141 caaccatacc gatgactgat gaagaagtcg ttcaatttgc gattcagcac ttaaaattta
    19201 cagattatca gacctctcat tatgattcat tgaactttac catattgact ggaattttga
    19261 tcaaattaac aaatcaaccg tatgaaaaat tattgaccga tgatatcatc gaacctttag
    19321 gattacagca tacaaaattt tatgaccaag tcaaaaattc atccaatcat gcactatcct
    19381 atcaaatgtc taaagaggat gattacttta aaccactgac agaatctgaa acagagatcc
    19441 ataacgaatt agggactggt aatatcagta tgtccgtcta tgatttaaat acgctgttta
    19501 cacaagtttt atctggaaaa ctagtttcca aagacttact cttctcatta tggcgtgaaa
    19561 atgcgaatgg acatccatat agtggtgggg tatatagcgg agataattat atcctgtcgc
    19621 aaggaaatat cgactctttt gatacatctg ctatatttag caaagattca caggatgcta
    19681 tcatcatgga aagcaatata aaagctgaca aaactatcaa actgcctact gctgatctaa
    19741 gaaaccaaat ctatgacctg atggaaggaa ctactaagtt ggcaaatagc tgatatgatg
    19801 aaaagatagc aaatactact tctttgaata gcatttgctt aaaaaaagta ctcgactaaa
    19861 cgtaggaaag agtgtgggac aaatctaaaa atcaattttt gctacagact ttaattccaa
    19921 ataaacagaa cagaaagagg tgttcttctt tctgttctgt ccaaattatt ctaagatttc
    19981 tctttaccaa cattatttga caaagggcct attaaatcta aaatagtatc tttagattgc
    20041 taaaatatgt gctagcaaat cattcatggc ttgttcttat ttttaatttt atgcaataaa
    20101 aaagaagaac aattagcgtg attatagaaa taaataatga gattttagaa aaaaatgtgt
    20161 atgaatacct gattgttact ttatttattc cactttca
//
LOCUS       DACDCJ010000054        19427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDCJ010000054 DACDCJ010000000
VERSION     DACDCJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.054"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(451..1761)
                     /locus_tag="IV038_001885"
     CDS             complement(451..1761)
                     /locus_tag="IV038_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8342090.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IV038_001886"
     CDS             complement(1773..3191)
                     /locus_tag="IV038_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8342091.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IV038_001887"
     CDS             complement(3431..4999)
                     /locus_tag="IV038_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP8342092.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IV038_001888"
     CDS             complement(5003..5413)
                     /locus_tag="IV038_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP8342093.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IV038_001889"
     CDS             complement(5410..6333)
                     /locus_tag="IV038_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP8342094.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IV038_001890"
     CDS             complement(6330..7208)
                     /locus_tag="IV038_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP8342095.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IV038_001891"
     CDS             complement(7195..8532)
                     /locus_tag="IV038_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP8342096.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IV038_001892"
     CDS             complement(8535..9638)
                     /locus_tag="IV038_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP8342097.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IV038_001893"
     CDS             complement(9712..11220)
                     /locus_tag="IV038_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8342098.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IV038_001894"
     CDS             complement(11186..11878)
                     /locus_tag="IV038_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8342099.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IV038_001895"
     CDS             complement(11900..12874)
                     /locus_tag="IV038_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8342100.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IV038_001896"
     CDS             13174..14046
                     /locus_tag="IV038_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8342101.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IV038_001897"
     CDS             complement(14504..16702)
                     /locus_tag="IV038_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8342102.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IV038_001898"
     CDS             16934..17926
                     /locus_tag="IV038_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8342103.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IV038_001899"
     CDS             17986..19407
                     /locus_tag="IV038_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP8342104.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
BASE COUNT         5818 a         4012 c         3244 g         6353 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatga
//
LOCUS       DACDCJ010000055        19129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDCJ010000055 DACDCJ010000000
VERSION     DACDCJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.055"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            167..745
                     /locus_tag="IV038_001900"
     CDS             167..745
                     /locus_tag="IV038_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP8342105.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            745..1674
                     /locus_tag="IV038_001901"
     CDS             745..1674
                     /locus_tag="IV038_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342106.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1671..3407
                     /locus_tag="IV038_001902"
     CDS             1671..3407
                     /locus_tag="IV038_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8342107.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3470..4306)
                     /gene="pheA"
                     /locus_tag="IV038_001903"
     CDS             complement(3470..4306)
                     /gene="pheA"
                     /locus_tag="IV038_001903"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP8342108.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4303..4812)
                     /locus_tag="IV038_001904"
     CDS             complement(4303..4812)
                     /locus_tag="IV038_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP8342109.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4805..6100)
                     /gene="aroA"
                     /locus_tag="IV038_001905"
     CDS             complement(4805..6100)
                     /gene="aroA"
                     /locus_tag="IV038_001905"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP8342110.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6113..7204)
                     /locus_tag="IV038_001906"
     CDS             complement(6113..7204)
                     /locus_tag="IV038_001906"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP8342111.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7221..8387)
                     /gene="aroC"
                     /locus_tag="IV038_001907"
     CDS             complement(7221..8387)
                     /gene="aroC"
                     /locus_tag="IV038_001907"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP8342112.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8389..9453)
                     /gene="aroB"
                     /locus_tag="IV038_001908"
     CDS             complement(8389..9453)
                     /gene="aroB"
                     /locus_tag="IV038_001908"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP8342113.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9486..10508)
                     /gene="aroF"
                     /locus_tag="IV038_001909"
     CDS             complement(9486..10508)
                     /gene="aroF"
                     /locus_tag="IV038_001909"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP8342114.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11167..12141)
                     /locus_tag="IV038_001910"
     CDS             complement(11167..12141)
                     /locus_tag="IV038_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP8342115.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(12122..14005)
                     /locus_tag="IV038_001911"
     CDS             complement(12122..14005)
                     /locus_tag="IV038_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP8342116.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(14023..16248)
                     /locus_tag="IV038_001912"
     CDS             complement(14023..16248)
                     /locus_tag="IV038_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP8342117.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16363..17397
                     /locus_tag="IV038_001913"
     CDS             16363..17397
                     /locus_tag="IV038_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8342118.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17464..18477)
                     /locus_tag="IV038_001914"
     CDS             complement(17464..18477)
                     /locus_tag="IV038_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8342119.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18555..18743)
                     /locus_tag="IV038_001915"
     CDS             complement(18555..18743)
                     /locus_tag="IV038_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP8342120.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         5389 a         4125 c         3492 g         6123 t
ORIGIN      
        1 ctaaaataat aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac
       61 caaagatcga aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa
      121 ataagtattt ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt
      181 aattcttatc gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc
      241 tgagcacggg tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc
      301 tttggtacat gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat
      361 cgaattatcg cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag
      421 ctatgatgaa tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt
      481 aaagcctcga ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat
      541 caaaaaaaag cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct
      601 ttcacagcag attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac
      661 cttgtttttc tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa
      721 aaacagtcga tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt
      781 tctacctttt cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg
      841 aagtttgttc aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat
      901 tcttttgcaa tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga
      961 caacgtttct atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta
     1021 accaaccacg agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct
     1081 ttagctgtca aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca
     1141 gcttatttga aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac
     1201 gagcatgttg ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc
     1261 gatgctgagt atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc
     1321 cacttttctg caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc
     1381 gtccaggaaa attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt
     1441 tcatatagct atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat
     1501 ctaaaaaaag tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg
     1561 acggaaatct gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa
     1621 gaagagacag gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa
     1681 aaatcaaaga gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc
     1741 tgcaagccat cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc
     1801 tgttcaatca atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga
     1861 atgatacaat cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt
     1921 tttccaatga tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa
     1981 tgaatgactc tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag
     2041 aaaatggaag aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt
     2101 ttcaatctgc gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg
     2161 cacaccacgg attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac
     2221 tgaaaaatac attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac
     2281 tagcgaatct ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg
     2341 aagaacagac gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt
     2401 tcaaaaaact aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc
     2461 ctctgtaccg ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc
     2521 aaatgggcgt gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct
     2581 cattggtatt tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc
     2641 taaaacggct agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag
     2701 tccaattgct agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac
     2761 agacgatcca agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga
     2821 tacagcccca ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa
     2881 aaacagatga agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg
     2941 ggaaaaaaga agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg
     3001 atcttttgag tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac
     3061 tgcagtcatt atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc
     3121 atgcttttca caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc
     3181 ttcttttatt cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa
     3241 aaaatgatca tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt
     3301 atggcgatgc atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga
     3361 tttgtctacc gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc
     3421 gctgattttt ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg
     3481 tctcttctac tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct
     3541 tgatttcttg gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca
     3601 cgaaaaaata ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga
     3661 tttcccgcca gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta
     3721 gtgtcaagat gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac
     3781 cgacgatcca aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct
     3841 ccaatccaac atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat
     3901 gttctgctac atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag
     3961 gataatgcgt ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt
     4021 ttgtaatctt tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac
     4081 ttttaacttc gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt
     4141 ccaatgaatt ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca
     4201 aacaaacagg gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg
     4261 cctgataagt gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct
     4321 ttctataatg gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt
     4381 cgcactttct tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt
     4441 ttttccaaga gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca
     4501 gtccgcttct gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc
     4561 tgcactttgg acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct
     4621 taaaatatct gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc
     4681 aatcgacaat tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa
     4741 acattgagct aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa
     4801 aatgctagcc atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg
     4861 gataagagat tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag
     4921 cagctacttg cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat
     4981 gcagaggtgt cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca
     5041 ttttctgtaa ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt
     5101 ctgcgtcttt gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca
     5161 attcatcaat cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg
     5221 tcttgacact taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg
     5281 cgcccatctc ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt
     5341 ttaacaacag ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg
     5401 aaatatcacc tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga
     5461 tcgttttatc ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt
     5521 tacgggaacg ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca
     5581 gcaaagcaga tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag
     5641 agcgggttcc tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag
     5701 cgcccatctc gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat
     5761 ccccgaacag agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg
     5821 tcgtaccgga gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc
     5881 gaccatgaat cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg
     5941 catgtaaagt actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt
     6001 cagcaattgc tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa
     6061 cagtccctct taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga
     6121 tccagcaatg ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt
     6181 ggtttttaaa actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca
     6241 aattaatcaa agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag
     6301 gttcatctgg cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga
     6361 caggcaactg gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt
     6421 tctcagtcat gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt
     6481 ccaataatat ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag
     6541 cgatcctagt gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt
     6601 gattgaattg ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta
     6661 gcattcctgt tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc
     6721 cgcaaaatag ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga
     6781 tgtaataggc attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac
     6841 cagccattgg atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt
     6901 gctgcttcgt actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa
     6961 ggacatcaag ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt
     7021 gttctgcacc tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt
     7081 gctcacgggt ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac
     7141 gaatacaaag tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg
     7201 ccatcttatt tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca
     7261 gctttcaact caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca
     7321 gtggctacaa cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt
     7381 tctacacttg ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac
     7441 aatgtaggaa tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg
     7501 ccttcgaagc cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg
     7561 atctcatcca tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct
     7621 acgcctttaa aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg
     7681 tcccattgta cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt
     7741 cctccgatcg tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca
     7801 actgctggat caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc
     7861 gagatatttc caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg
     7921 atgatgtcaa gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc
     7981 gtctctcttg cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata
     8041 ccgcctacca aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct
     8101 ttttcaggca cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct
     8161 accactaaag tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga
     8221 acacgatctt tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg
     8281 agttcgtgat tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg
     8341 gctgttaatt ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc
     8401 taagtaatct ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc
     8461 tagcaagatg atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa
     8521 taattcatct ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct
     8581 ttctaaagca gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat
     8641 ggctaccatt ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt
     8701 ttcaattgca tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa
     8761 gacatcttct tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc
     8821 atgttccaga agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc
     8881 tgctatagct gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa
     8941 agtttttaga acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag
     9001 gtttttcgct ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc
     9061 aagtagcgta gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa
     9121 tgctgctaga tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa
     9181 atcattatct gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc
     9241 acccgccggt acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat
     9301 tttttctgca tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct
     9361 ccaaatcgtt tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc
     9421 gtatttttgg ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc
     9481 tggaattaat cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc
     9541 atcatgcgtg agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca
     9601 ggatcaggat ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc
     9661 attggtggta caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa
     9721 tgagttaatt ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt
     9781 tcgtaagtcc gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg
     9841 tattctgcag cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca
     9901 ggtttacctg ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca
     9961 atctgtagga tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca
    10021 gtaatcactt tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct
    10081 tctaaacctt ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt
    10141 tttgcgcccc cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata
    10201 gaacaaggac cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc
    10261 acgactgtat tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc
    10321 acagcatttt ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca
    10381 ccttttagtc caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct
    10441 ttttcgatat gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt
    10501 actatcattg tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta
    10561 ctttttcaag gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt
    10621 ttcgctcctt ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg
    10681 aaattgcctc tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa
    10741 tagccactat agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca
    10801 tcatagatac aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa
    10861 atagctaaag taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc
    10921 caaactgatg taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt
    10981 tatgaaactt tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg
    11041 ttttattgaa gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc
    11101 atgacatata gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa
    11161 agattgctac tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg
    11221 tgactcaaca tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat
    11281 cgtaagaacc gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc
    11341 ttctgctttg aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa
    11401 cgaaaaatct tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac
    11461 ccatttcgtt tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag
    11521 tcctgccact ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc
    11581 tgataggcct tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa
    11641 tgcgggtaat tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc
    11701 ctctccagcg atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag
    11761 tgtaaaaatg atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg
    11821 agcaatcggc aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg
    11881 atacaatccg gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt
    11941 atcggtcgtt gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt
    12001 tttattgagc gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga
    12061 tttgacacct aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact
    12121 tttatttact gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc
    12181 catctgcaaa aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt
    12241 caccgccata gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat
    12301 ctttaaaatc ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt
    12361 aagaatccat tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg
    12421 gtttttccgc tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc
    12481 tttttacatc tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga
    12541 caagtcgatc ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg
    12601 cataagattc atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga
    12661 aattcgtttt tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt
    12721 tgtaagaagg ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt
    12781 ccgccatcac atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt
    12841 aatgagatgt ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca
    12901 tatcttctat agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt
    12961 tgataatcat ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa
    13021 aaccgaagga atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt
    13081 caagcttata taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga
    13141 tgcgaatcgt catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag
    13201 gatctagtat cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta
    13261 cttcaagttt gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta
    13321 aatggttttg tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa
    13381 agtcctgatc ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat
    13441 tttcttcttc caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat
    13501 acccgataaa atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa
    13561 tcgagatttg ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa
    13621 ccggattgtc tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga
    13681 aagggggacg gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct
    13741 tgtcataatt tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat
    13801 caaaatcttt tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac
    13861 gccatgatcc atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg
    13921 cataacaaac atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat
    13981 ctaaccattt tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg
    14041 aagcagttct gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa
    14101 ttcggccccc gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa
    14161 ccccatcaga tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa
    14221 cacaatagct tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa
    14281 aggactcgcc aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga
    14341 tataatttgg tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc
    14401 ataaccaaaa acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc
    14461 ggtctgatga tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag
    14521 gcttgttcca tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga
    14581 ctgcggcata taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc
    14641 ccataagatt tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat
    14701 ataacgatgc gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga
    14761 aagcgaacgg ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct
    14821 catttgtttc tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga
    14881 tctggaagca gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata
    14941 cagttcagaa tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt
    15001 gctatgagca taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa
    15061 ccagtctcct aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac
    15121 gaacatttcg atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt
    15181 gaagtcaaag taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg
    15241 acgacctctt gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa
    15301 tccctgactt gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa
    15361 ttcccacgaa aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc
    15421 tttttcaagt tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc
    15481 agtattttct gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct
    15541 cgttcctcga cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg
    15601 tctttcattg acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc
    15661 aaagtctagc tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct
    15721 tatctttaca gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa
    15781 ataaatcgct gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc
    15841 catcacataa gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct
    15901 gtcatatctc agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt
    15961 tcgaaagtcc atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga
    16021 ccggtcagga tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt
    16081 attgtctttg tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc
    16141 ttctatcttt atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc
    16201 atcaaataag atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc
    16261 aacaaaacgg aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt
    16321 ttgtcaataa aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat
    16381 gtcgcacgaa aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag
    16441 acgatccatg tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat
    16501 tatgacatca gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct
    16561 acgatcggca aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg
    16621 gaagaagaag ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat
    16681 cctaaacaat gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg
    16741 aaagaatcga ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct
    16801 gacatccagc aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta
    16861 ctggaattgt tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc
    16921 gatatggatg aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat
    16981 cagcatttta tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg
    17041 acagaaaaac aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct
    17101 actgccattc ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa
    17161 aaaaatatcc aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc
    17221 gcaaacctct cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca
    17281 gctgttcgaa tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact
    17341 tatcctgcac gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag
    17401 agaggttgtg aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac
    17461 ctcttactta tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt
    17521 tagcgtgtaa tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa
    17581 ggattgcacg ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg
    17641 gaatgagtat gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc
    17701 agccacacta gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga
    17761 ccacgcaacg gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt
    17821 tagcttcaaa tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag
    17881 tagctgtttg attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg
    17941 caatgcgatt tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa
    18001 tttgtcgtct ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg
    18061 attagggtat tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc
    18121 gtctgaaact aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc
    18181 tccaccatgt acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat
    18241 gtcataagta ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt
    18301 tttttctgct tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg
    18361 gttgatcaag aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc
    18421 gatcccaaga atgattccga agaaccatcc gataaattta agaattttct ttttcataag
    18481 gctccctcag tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag
    18541 ctctcctaga tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga
    18601 aattttttcc attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat
    18661 tgaggactta agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca
    18721 atcacttgct ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac
    18781 taatttatct tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta
    18841 ttctaaataa taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat
    18901 cccccctgat gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt
    18961 tgaatcattt gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa
    19021 ataacgaaaa atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac
    19081 tcaacagtcc ttattggcaa atcgccaaaa taatgtagac taactaaaa
//
LOCUS       DACDCJ010000056        18929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDCJ010000056 DACDCJ010000000
VERSION     DACDCJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.056"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..462)
                     /locus_tag="IV038_001916"
     CDS             complement(<1..462)
                     /locus_tag="IV038_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HAP8342121.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKT"
     gene            545..694
                     /locus_tag="IV038_001917"
     CDS             545..694
                     /locus_tag="IV038_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HAP8342122.1"
                     /translation="MKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPL
                     ENELR"
     gene            complement(1443..2056)
                     /locus_tag="IV038_001918"
                     /pseudo
     CDS             complement(1443..2056)
                     /locus_tag="IV038_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3277..3981)
                     /locus_tag="IV038_001919"
     CDS             complement(3277..3981)
                     /locus_tag="IV038_001919"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8342123.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            complement(3996..4865)
                     /locus_tag="IV038_001920"
     CDS             complement(3996..4865)
                     /locus_tag="IV038_001920"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342124.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            complement(4973..5989)
                     /locus_tag="IV038_001921"
     CDS             complement(4973..5989)
                     /locus_tag="IV038_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8342125.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            6179..8902
                     /locus_tag="IV038_001922"
     CDS             6179..8902
                     /locus_tag="IV038_001922"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8342126.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            9030..9452
                     /locus_tag="IV038_001923"
     CDS             9030..9452
                     /locus_tag="IV038_001923"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAP8342127.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            9470..9940
                     /locus_tag="IV038_001924"
     CDS             9470..9940
                     /locus_tag="IV038_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8342128.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            9964..10746
                     /locus_tag="IV038_001925"
     CDS             9964..10746
                     /locus_tag="IV038_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8342129.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            10736..11560
                     /locus_tag="IV038_001926"
     CDS             10736..11560
                     /locus_tag="IV038_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8342130.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            11595..12593
                     /locus_tag="IV038_001927"
     CDS             11595..12593
                     /locus_tag="IV038_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8342131.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            12610..13464
                     /locus_tag="IV038_001928"
     CDS             12610..13464
                     /locus_tag="IV038_001928"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342132.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(14038..15288)
                     /locus_tag="IV038_001929"
                     /pseudo
     CDS             complement(14038..15288)
                     /locus_tag="IV038_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            15805..15888
                     /locus_tag="IV038_001930"
                     /pseudo
     CDS             15805..15888
                     /locus_tag="IV038_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            15977..17388
                     /locus_tag="IV038_001931"
     CDS             join(15977..16532,16532..17388)
                     /locus_tag="IV038_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8342133.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            17463..18746
                     /locus_tag="IV038_001932"
                     /pseudo
     CDS             17463..18746
                     /locus_tag="IV038_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         6228 a         3035 c         3643 g         6023 t
ORIGIN      
        1 tgtcttagta tataaaccca cactctgtaa ctttctaaag gcggagccaa gagaaggtgc
       61 tttatcggtc acaattgctt tcggctcacc aaactgttta tggagtcgtt ttaagaaagc
      121 ataggctgct tgcgtatccc gtttctttcg taaccagata tctaaggtta agccgtccgc
      181 atcaattgca cgataaagat aatgccaacg tcccttaatt ttgatatagg tttcgtccat
      241 tttccatgaa tagaaggatt gtctattttt cttcttccaa agataataga ggactttgct
      301 gtactcttgc acccaacgat aaatcgtagt atgacaaaca tttattccac gatcatataa
      361 caattcctga acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcgacaat
      421 aatgacgtct tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc
      481 ttttttctca attttacact aaaatagatt ttttggaaaa ctttgcaaca gaaccatttc
      541 taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca aacatccaga
      601 agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa tgttagatga
      661 tgagttgatt ccacttgaaa atgaattgag gtaaatgaga agagttaaga aaattgtacg
      721 attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct tttatttgtt
      781 cacattttat aacgaagtat ttaagacagc tattgaataa tgtttcctcc acctttttca
      841 ttcatttacc ccaccccgtt gttatatgac cgtgtttcga gagaaaccaa taaaaagcat
      901 aggctaacta ggggttcctt tttcttttga ttttttggaa tccgcttaag tgaaatgtat
      961 ctgaaatgaa acagagataa agtgattttt tgctgtaatg acaaggtttt agctgtttca
     1021 gataccatgt aacccaaaat agtttattga gaaaatagga gagtatttga tactaactag
     1081 taggaaaaaa tgagataaaa agtaaatgga atcatttcag gtaatctcaa ataaaaaata
     1141 tacaggcctg tggcacttta cctacgggac tgtatatttt ttactctcag cgggcttgtt
     1201 ttgactccgt gtttgactcc gtcgtattga ttttaaatga tattcgatga ccaattaata
     1261 ggtcgttttt gaaatttttg tctctatatg actcagtttg ataattattg atagatcgat
     1321 ttgggtctat atcgaacaag ggaagattgc aaaagtcgaa gcttaaagga ttgatattaa
     1381 aggattaaga ggtaaaaagg tatccccata atatcctctg actccgtttt tgacctcgct
     1441 tatcatccgt tcatgtaaga atcaaataat tcgactgttt cattttgccg attttttgat
     1501 actttaatat aaatgtccat gattgtttta gaagaagaat gtcctagcct atcttgtaca
     1561 tcttttactt gagcacctgc ttcaaagaga agggtagcat gagtctttct gaagccatgg
     1621 actttgatta tcttcatatc tgggaacttc cgtgctactt tatccatgac atggttaggg
     1681 tagcctttgt aaaggaagtc ccttttttta ttccctttga tagtgctgat tacaggtata
     1741 tcatctatat tatctatccg aatccctcta gaaaggttct tttatgttac agaacgtttc
     1801 cattttttag aatacgtaag gttccattat caattgaaat tactcccttt gatttgtctg
     1861 tttttgtagg attaatacat agagagccct cggatagttc tactaacgtt ttgttgaagc
     1921 agatagtttg attatcgaaa tctatgtctc tacatgtcag tccgagtatt tctccttttc
     1981 gttcccctgt ataagaagct attctaaaga atactaaagc acgctcatct tttaacttat
     2041 gcaaacattc aaaaaacaga tatttcacat atatctaaaa ttcggagatt gaaagaatga
     2101 atttatctaa gaattttcac taatgttgcg aatataatat cagaaaattt cgaaataaat
     2161 tttgttaaaa attagaaaaa tccgaatact tagtagtgga taaatttttt tcaggaatag
     2221 tacgttttat taaaatgaaa actaatccga catcaacttt tttatcgaag caaacatcaa
     2281 gcggtaaaaa gacctcttta gtttgaaaag tgttgctttc atattgattt aaattttggt
     2341 taagtaggta aattaagaaa tgatcagtgt aagtatttcc tgatttagcg caccttgttc
     2401 actttgaaaa tacaggtttt tgatggacag attttgcttc aaatgtttaa agaacatgcc
     2461 actgtacttg gtacaggtcg gcgcccccat ttggagttgc gtcagatcga gtcgtgacga
     2521 ttcattttgt acaaattaga atttaaaatt gaatacatga cgcgcacatt ttcccagaga
     2581 gccatattct gtaattaatc acagcaatta gtgattatgc acaatctaaa aaactgattt
     2641 ataataaaaa tattattcaa ataaataata ataactgccc gcatgatgtt gacagttaca
     2701 atagcacata gatagactgg caaagatggt tcttaatctt tcactttggt cagaattctt
     2761 gggttatcct tccagtgaaa gtcagcagaa aatccttaat gcttacaaat aatttttgcg
     2821 aaataggttt tattggtaaa ggactcaatc atgaatatga attactttta tctggtaaat
     2881 ttggaaaaat tttgcaaaga aataaaaatt agacaaattt gctacacata atcaaagatt
     2941 aaaaaagttt ttaaaatata gttgtttgaa tggggatatg aattagagaa aattggttga
     3001 ttcaacgggt gctatcattt ttgatagcgt catatgtgaa gtaagcacac ctaatgaaaa
     3061 aggttcaggc ttgctttgaa gacttattaa caactatgtc gactatcctt gacttagggt
     3121 agaggagtac ctccacacac cttaattaga tgataatgtt tggactgtag gctacttagc
     3181 ggattaagat aaagaggaag tagaaaaaga gccttgcgga aagacacttt atctgttatc
     3241 cgcgaggctc tttatacatt taagcatatt taaaaattat ttggaaacag tactttcaaa
     3301 ctttgtctta taagcagggt acttagatat ttcatattca aacggtttct gatttttagc
     3361 aaatccaata cttgtaaaga caaagactgg ctcatttttt tctaaatcaa gataaaggtt
     3421 ttctaaatct gttggcggtg taacttcaat tgagcgtttg gcataagcta atctaattcc
     3481 cctactttct aatactgaga acagggattc tttggcaaaa tcatgttcaa caatttcagg
     3541 taaaacagat aggggaatgc aactttttgc aatacaagtt ggatagtcat cgatgtatcg
     3601 aattctttcc aagacaagca ctaaagtatt ctgctcaatc tccaaacatt cactaattgt
     3661 tgaatcagca ggtatgatgg tttgttggat aatcttagaa ctgattttct ttccttgctg
     3721 aaccgcatct tggaagaatc cgataaaaga gtcgagttcg tggtcaacat atttttctga
     3781 agctggtttc actagatacc cgcgaccttc atttttagaa ataattcctt cgtcttctaa
     3841 agaagctaaa gctttccgaa ttgttggacg actcaccgaa aattcctggg ccaaatctct
     3901 ttccgaaggc aataattcag atttccgatc agtggtgata tacacttttc gaatttgttc
     3961 ttttactttg tcaaaaatca tagtaatcca tccttttacc aaggtattaa cattatatta
     4021 ccattttttt gacttaatat cccagatttt ccttcaatta attccttcgc cgctaattga
     4081 ccccacaaat agccaatata tcggtcataa aaagttggag atgatccacg ctgaagatac
     4141 gcaagattta agtatttggt ttcttttggt aaatttttgg taataaattc aatgattttt
     4201 tgtttgttag atataccttc tggtaaaagc aatataggcg agtagaactc cgactcaaat
     4261 tttttacata ttaagtgtaa gtattccttg tcgtctctct caacccaata agtattattt
     4321 gcaatggaag cgtactctgg aagagcaaag cagcctcggc ccatgacttc cattataata
     4381 atacgatcat gagcattagc agtagaagtt aaatcatcga ttgatcggat tatagtgttt
     4441 aaggcgctat cgaagccaag tgtataatcg gtattgggga aatcgttatc aatagtccct
     4501 gggatgacag atacttgtaa ttctgattca tttttcaata gttcagctcc tttcaaggaa
     4561 ccgttacctc caataacaat taagttgtcg atctccatct tatttaggtt ttcaataata
     4621 attttccgac tttcgagatc tctcattttc tcaaatctgg ctgttccaat aagtgttcca
     4681 gctttgaata cctcaacttc ttttagagaa actgaggcaa tacattcctt aaatattccg
     4741 gcataaccat tcttaaaaaa gactacttcc gaatctggtt cgctatcaat tgtttttttt
     4801 attgctaata aagctgaatt cattccggga gagtcgccac cactacagat aactgcgatt
     4861 ttcattttag atcctcctaa aaaaggtgac tgcgattcta ttctcccatt agagaaaatg
     4921 aagcaatcaa atgggcaatg gagtcgcggt cacctttttc atatttttat gattagtatt
     4981 ccatttgctt ataataacgg cgagtagtta aagggtggtt acgttcttct tctagaaaag
     5041 cactaattcg ttcaaagaca gtagctaaga cgactgggga aataattttt cgcgtgtctt
     5101 ccgaaatgcc aggtaagtca tagtcctttg tatcaaccgt tacgtgaaca tctgtaaact
     5161 taggaacgaa attcttcacg cgttctgtga gaggtcgaga atcatcttct ccttctgtaa
     5221 tgatgacact tacacccgct tcaattaatt caagagttcc atggaagaag tctgctgcat
     5281 gaacaggacg agtcttaatc cattgcatct cttctaaaat acacatgcca tagtaatatg
     5341 cttccgcaaa gctatcacca gcaccggtta aaatatgata tgaagtatct ttgtgttttt
     5401 ttgcaaaaga ttttgccatt ggttccgctt gctctttagc gtgcactaag gcttcaggta
     5461 ttttttttaa atcctcaata taggtattgt attgattgat atcgatttca tttctttgtt
     5521 tcattaagaa gagagcaatc agcagtcctt gaaggtaata attttctgaa gatgtatcat
     5581 ctaaaacgtc gttatagata gaatatgttg cagctttttc taggggcgtt ttaggacttc
     5641 ctaccaaagc taatgttttg acgttcattt cattacagta ttcgactaac tcaagtgttt
     5701 cttttgttgt accagataaa gatgggataa caaccaaaga tttgttagaa aatgttttat
     5761 tgggatgaat cataaattcg gcagcgataa cacgatgaaa agggaaagtg ctacgagttc
     5821 tcgcgaattc gattgttgaa tcaaaaagaa taccaactcc tccagaccca ataaagtaaa
     5881 tttcttctaa gtcagatgcg atggattcta aaatattgga aatttgttca tttgttgcaa
     5941 cggcaccagc ttgagtcttt aaaaatcttt cttgatcaaa attaaacata aaaatctctc
     6001 ctttgattac caatggtaat attttgtttg cataaataaa ataccacagg taataaaaag
     6061 tgtcaaatat tttttaggaa aaataccata ggtattgtta aagtggaaag atgtttataa
     6121 agaatattga attttatagt gaaaatcatt tattattaag taaaggaggt gggttcattt
     6181 gaaaacgaaa attttaaaag aattaaatga tctacttaac agctatgatt ttccacaata
     6241 ttttccagaa gaatgtaacg cgcaatatat tgcccaaaaa tttcaaataa aaagaaatac
     6301 ggctagtaag catctaaacg aactaatgga acagaaaaaa gttgtgaaaa tcaatggtcg
     6361 tcctgttctt ttttttccag tccatacaat tgaacggtat atatcacatt cattagatag
     6421 ttttgagttt tcttcaattg attcgtttag agaaaaaatt tcaaagaaaa aagcagatca
     6481 tttttttgat gatttaattg gtgcaaaaca aagtttaaaa gaagcggttc aacaagggat
     6541 atcagcaatg aaatatccag gtggattacc aataatgtat tttggatcgt ccggagttgg
     6601 gaagagttac ttagtggaaa aactagctga gtattgccga caaactggta ttattcatga
     6661 gaatgctccg ttcatcgagt taaactgcgc tcagtatttt aataatccag agttattaac
     6721 aagtcagttg tttggccata tgaagggatc gtttaccggc gccgattctg accatattgg
     6781 tataatagaa gcagcagacg gtggaatcgt ctttcttgac gaaattcatc gtttaccacc
     6841 agaaggacaa gagaaacttt ttattcatat ggataaaggc gtttttcgaa gagttggaga
     6901 tagtggtcct tggcgtttat cacatgttag atatattttt gcgacaacgg aacaggaaga
     6961 aaattttttt ctagaaactt ttcgacgaag aattccgatt gtttgtactt tgcctaatta
     7021 ccaagataga gaaattagcg aacgaaaaga gattgtcttc catttattag agaaagagtc
     7081 tactctgatc aataaagaaa tattagtttc tgaagccgcc cttagtttcc tgattcgctt
     7141 agatttgtat ggaaacattg gtgagctgga tggactaata aaacaaacta ttgcgaataa
     7201 actatttagt ggaagcaata aaaaatatat cgaaattcat atttctgatt tacctagcag
     7261 gtacttgcta acagaaaata ttgagttaaa aaaccaaatt gcgagccaac ggttctatat
     7321 ctttagtaat ggtaactatg ttcatgattg tgacgagact tcaactgtaa ctacaaattt
     7381 acgaggtttg attcatcgtg tttctaaata ttattcagag tgtcatgatg tgaatagaaa
     7441 tagtgaagag tttcgacgtt ttgtgaagga ccaaatggca gaatttaatg atcgttttat
     7501 ttaccaaaaa aattcagaag tcttagttaa gtattattta ccggaaatcc aaaatattgt
     7561 tcaaaaaatc tctcccagtc tttttgttca cctttctggc aatacggtcc aagctatagc
     7621 tacctatctc gctattaggg atcaatgggg gtatgggaaa gagaatgctt cagcgaatga
     7681 aattgaaaag ttatatgaaa acctaaagtg taactatttg acggatttaa atgtattctt
     7741 actgatcttc gagaacgccc tggatacaac attgactatt tgggataaac tgtatatcag
     7801 tttgtgtgtt attagtgaga ccggaaatga agcgaaagac attaacgcaa ttattttagc
     7861 ccatggatat gcgacagcta gtagtattgc taatgttgcc aatcgtttgg ttaatcagtt
     7921 tgtcatagat tctattgata tgcctctaga tatcagcttt agtgaagtaa ttgacagatt
     7981 acttcactat atagagtatc gaaagcctaa agatggacta gtgattttcg ttgatatggg
     8041 aagtttagct caaattaaga ctgaaattga acaagtaatc gaagttccaa caatgattat
     8101 taataatgtt acaaccgaaa tggctattga aaccgcacaa ctgattcaaa gtacgagtga
     8161 tatccaaaag gttgtaaaaa aattgccgta ttctcagttt gaaaagcaag ttctatatcc
     8221 catcaaaatt agaaaacgta caatcgttgt ttcctgcaat actggtttgg gtacctccat
     8281 aaaaattaaa gaaatgatgg aaaataattt gtcgaaagac ttgggcattg aatttctgcc
     8341 atacgaaaat gaaactctta gagatactca gcaattagag tttctcatta aaacaaagga
     8401 tatcattgcc attatcggaa cagatgatcc gcgtgtgtcg aacattccct atatttctct
     8461 tgaagaatta tttgatggtg aaattggcaa actctataaa gttcttttga gagtctatgg
     8521 aaaagagatt gctgaggcaa ttaatcaaaa tattgttaaa aatagctcat taaaccgctt
     8581 gattgacagt cttactattt tagatgccga aaaagttgtc aatcaaattg aggaaagttt
     8641 aaaagattat caaatgaggg tggggaaaac gttatcatca ttgtccaaaa ttaatcttta
     8701 tgttcatatc agctgtatga ttgagcgact aattcgtaat cagggagtaa gtagttatcc
     8761 agcaatagat cagtttctta agcgatacga aactgaggtt gcttctattc aaaaggcact
     8821 tagtaagatt gagaaacttt ataatattaa gattcctatg gaagagattg catatgtgtg
     8881 caacatcatt gtaagttatt gataaaataa agtgacacta ttcacagcga tggaaatagt
     8941 gtcactttat tttttgtacg ccctttcatt taagtggtga cagtaattgg cacagtattt
     9001 gctaataaca aagcgaaagg agttcgggta tgagacaaat attatgtatg agccacggtc
     9061 ctctagcaaa aggaatgatt gaaactttga gtattatcgt aggagatttg gataatattg
     9121 attttcgatg tgcctatgtc gatggaaata atgacgtaga aatgatcatc gatgattatc
     9181 taaaggaatc tatgggaaat gagacgattg ttgtaaccga catttttggt ggaagcatta
     9241 ataatgagtg gctaagacgg ttgccagatc tttctaaagt ctatttgatt tctggaatga
     9301 atttaagtct tttagtggat ttatatttaa aattccaatt agtttcatct gatcagattg
     9361 aagaaattat tgatcatgca attcaagcat cttctggttc gattaagttt tgtaatcagc
     9421 tagattatga gcaaacggtt gatgactttt aaataaagga gggaagagaa tgattaaatt
     9481 agtgaggatc gatcatcgtt tgatacacgg tcaagttgct ttaacttgga cgaacgcaat
     9541 cagtgcggat tgcattttag tagcgaacga tgaaattttg aatgatccaa ttcaaaagac
     9601 aactttgaaa ttagccgcac cacataacgt gaaggttgtt atcaaatcaa ttcaagattc
     9661 gattgatgca attaatagtg ggaagacaga taaatacaaa ctatttgtag ttaccaaaac
     9721 catttccgat gctaaacggt tggcaaatgg ggttgaacaa attaaatctg taaatatcgg
     9781 tagtgttaag gaaactagtg gcagtagcgc tttgacgcat acaatttttg taacagaaga
     9841 ggaacgagca gaacttaaag gaatggtctc agagggcatt gaagtgttcg ctcaacaagt
     9901 tccaacagat aaggcaatag atttaaaaaa atctttgtaa taaaaaaata agggggtaat
     9961 ttcatggata gtctcttagt acaagcaatt ttgcttggtc tcttgactgc gttcatcgta
    10021 attgactgga tgacaggtac gaataactta tcaagaccaa tcgtatcttc cgtctttgtt
    10081 ggtttaatta tgggcgatct taagatgggg attatgatgg gggcaacact ggagttagca
    10141 tttatcggag caataacaat cggagcggca cgtccgcctg atacggtatc tggtgggatt
    10201 ttagggactg cgtttgctat tgcaactggc aagggagcag aatttgcgtt gaccttggca
    10261 ttcccaatcg ctgcgctatt cttattagtt gataatctac taacattgct tgttctacca
    10321 atgattgcta gaaaggcaga tagatatgct gaaaagggcg aactaaaaaa tgtgtcgaaa
    10381 atgcatgttt taggttttat cttagtaaaa agtttaccac gggctttatt tgtcggattc
    10441 tcattttact taggaagtcc tgttatggaa cgaatcttaa atgctattcc aacttttgtc
    10501 caagatggga ttaacattgc tgcaggcatt atgccagcac taggttttgc tattttattg
    10561 caaatgcttc ttaagaaaga tgtctttgta tatttcgtaa ttggttttgc actctttagc
    10621 tacttacatg tgccgattct ggggattgca atcttcggcg catgtctagc ttatattata
    10681 gtaaacatgg aaaccaaagc tgcaatgggt tcaagagggg aggagatggt tgaagatgac
    10741 ttctagtggg aaagctttaa ctaaagatga tttaacaaaa atttgtattc ggtcatgtgc
    10801 attagaagca tcatggaatt ttgagcgcca acaacatatg ggctttgctt ttgctatgat
    10861 gccagcgtta gaaaaaattt atagtggcaa tgaagaagaa aagcaaaaag cagctcaaag
    10921 acatctagag ttttttaaca cagctccgcc attatcaaca ttcataatgg gaattgtggc
    10981 ttctatggaa gaagaaaatg ccaaaaatcc tgatgacttt gatacttcta caatcacttc
    11041 tattaaaact gcattgatgg gaccattagc tggtattggt gattcaatgt tttggggtac
    11101 cttattagtt atcggtatcg gtgtaggtgc ttcattagca aaagaaggca atattttggg
    11161 tccattttta tttttactaa tcttcaatgt ccctaatttt cttctccgtt actttggagt
    11221 tatgcaggga tataactttg gtacaaagct attagagaac attgaatcct ctggcgtcat
    11281 gaaaacgttg actcatgctg cctcaatttt gggtcttatg gtaattgggg gcatgtctgg
    11341 aagtatggta tcaatcaaag tagctggttc tattggacaa ggtgatgaag caacaaaaat
    11401 tcaaagtatc attgatggta ttttaccaaa tctgctgtca gtattatgtg tatttctgat
    11461 ttactggatg cttaaaaaag gctttaaaac aacttacgtg ttattcggaa tctttatctt
    11521 tggtatcata gcagccttct tcggattttt gacaacttaa ttgaataatc taaataattg
    11581 taaggagaga actaatgtta aactataatg caaaagaaat gaaaaaaaaa ggcaaacgga
    11641 tttacggtat tcgtgaagat gtagaaaaaa ttgcaaagca agcttatgat tctggaatta
    11701 gtaatatttt ctttactgct tctggtggat cggtagccgt aatgcaacca tttgagtatg
    11761 tattgatgac tgaatcaagt attcctgttt actctatgac tgcagctgag cttgtcttaa
    11821 tgggacataa acagttgaat gaaaaatctt tagtaatctt atactcaaaa tctggagata
    11881 cgaaagaaac tattaaggca gctgaatatt taaaagaacg aaacgtgcca accgttggta
    11941 ttgtaagtaa agaagataca ccgttagagg ctctatcaac ttatgcaatc aattacctag
    12001 acggccgccc tcaagaaatg ccactttatt tcttaattat gtctatcatg aatttgaatg
    12061 gggaatttcc tgaatacact cgttttgctg atgaactgaa aaacttacca acggctctga
    12121 attcagttag agaacaggct gatgaaaaag ggaagaagta tgccgaagaa tttaaagaag
    12181 atccttatca aatttggatt ggtagcggga atttatgggg aatcacctac tcttattcaa
    12241 tgtgtgtgct tgaggaatct caatggttgc gtacaaagtc tgtctcatca ccagagtttt
    12301 tccatggtac cctcgagtta gtcgaaaaag gagtttgtgt aacacttttg aaaactgagg
    12361 gggaaacacg gcttttagat gctcgagttg aaaaatttgc ggtccaatat acagataaat
    12421 ttaatgtttt tgatactaag gattatgagc taccaggtat tagtgacgaa tttcgaaagt
    12481 acttttgtgc tccagttatg tgggcagccc taggtcgcgt cagtgttcat cttgaagata
    12541 ttcggaacca ctctttggat aaacgtcgtt attaccgagt agttgaatac taaaagtagg
    12601 tgattaatta tgacagcgta tgtgattgga tttggcgata atgttgttga ttactatgag
    12661 aatttgaatc aaaaatttcc agggggaaat gcagttaatt ttgctgtgaa tgcaaaaaag
    12721 agtggtgtta aagcatacta tgtcggtagt ctatcatctg atcctgatgg cgtgttatta
    12781 caacacgcca tcagttctga aggtgttgat atctctttct cggaagttat tgacgagcct
    12841 actgagcaag cacatgtaac tattaaagat ggagataggg agtttatagg tggcgtgcgt
    12901 ggctcccgat tgacacctgc tctctcagca gaacttattg atttgatgaa gaacagttca
    12961 ttagttcata ctagttgcca tagtcgaaca gaaacaaaaa ttaaagaatt aaaaaaatat
    13021 gatgttaaag tttcttttga tttttctgac ttagataaat atagaacgca tgaatacttg
    13081 tcagatgttt gtccgtcaat ttatattgca cagttttcag tcgctacaga tgatcaagag
    13141 gaaattaatc gcttaattag tgaatgcaag gactttgagg ttccgtatct tctttttacg
    13201 cgtggtagta agagccctgt ttttgttgac actcgagaga ataagactta tgaaggattc
    13261 attcgcaaag ttgagtcacc tcatgataca atgggcgcag gtgatgcata ctttgcagct
    13321 tttgcaagta attttgtctt atctaaacgt gaaaaatcaa tgatagaaca tgttattgat
    13381 tgttttcaca aaggtgcaaa ccttgcatat gaaacattga tgattgatgg ctcctttggt
    13441 catgctacac ctattaaccc ttagctttgt gctttgggct tttattgctg gaatttgtat
    13501 agtagactca tcataactat atttttgtat gtgaaaaggc aagaggctat gatgaaaata
    13561 acactggcta attgcatgag ggagcatatc gttgttaaag cgtcattgat agtatgaata
    13621 aatatctcga taatattata ttgttattta cttaatttat tgattagtca agcaaagata
    13681 atcttctgtt acttaacgag actagtaggt acaagcactt catgttatgt acgacccaaa
    13741 aatattgctg ttgattattt gctgaaatta ttgcgagctt caatttattt ttaaaggact
    13801 tggtaggctg cgtgatgaaa taagacttgg aaggctgtct aaaccaatat atagtagact
    13861 aaatagtaaa aaagagcatg gtaattgtct ctttgtcaat aagtctaatg gtaataagac
    13921 aagttggaga ttgagaatat ttttattgtg taacctattt tgaagcgtac ttaactagcc
    13981 ccggccaaaa tcaaattttt tcggggtata tcaagggttt gcgtcaataa cagaatgtta
    14041 tttatctatc acgttccttg gtggcgtgta gagttgatgg ttacgtagta gcgtatctac
    14101 cagacgcgta aattttcttg cggttaagac gtgtgctcgt ttgtgtttat ttttgggacc
    14161 tctttacatt tgctttgata aaaagcttta tattgaggaa gatatttttt tacagagttg
    14221 gaagcttcaa ctaagtagta tctaaaatag cgactccttt gttttgtcag aggtgtattt
    14281 tgtgaccgat agttaacaga ttgtttgcct ttccaacata agcctgcgta ttttgccaat
    14341 tttgtttgat cttcaaatcg ttcaatttga ccgatttcag cgattaatcc agcggcgtgt
    14401 acctttccaa tacctggaac actagtcaaa tattgatatt ctggaataac gacaacaaat
    14461 tgctcggttg ctttatccaa gtctttgatg ttcttttcaa gcgcacgaat ttcacataca
    14521 aaaacattta gaacgacatt gatggattct tatgcaagac tatctaaccg ataactcacg
    14581 gcaattgcac gttgaatcga tttggcattt ttttctggat ctttaaagcg acatttcctt
    14641 tttcttttag taagtcacag aaagcttcca aaggaagcac agccaattca tctagcgtga
    14701 agtcttctgt cacaagagta atgacagtgg cactatagag acttgtcgat tcatcgcgca
    14761 tttatcgagt aagcgtattg catttgtacg ttagattttc cagaaagtgt tgttttgtac
    14821 gagtgaagtt gcttaatgag tcgatagcga gtgcgtgtta aacgctgtaa agccacgtac
    14881 ttctcttctt tgataggaga agttgtgaac cgttgaatac gcaataaatc agcaattctt
    14941 aacgcgttgt ttcgatcagt cgtatcttcc tcaaacatgc gactgaattg tttcatccga
    15001 aaaggatttt caatcctcac aagtgcattg agttttttta gctctgcatc ttcatggaaa
    15061 aacatcgaag gatgaaagct gtacatggac gtttaattcc atctcaataa cgatttgacc
    15121 gaagtgattg ttttcattga acacaagaat cagttctcgt gtcaatggag ccctttcaat
    15181 gtcattagca acagaatttt cagtcaatat agatagctga tcgtcgtctg ttaggaagca
    15241 aacgtctaat ttttcagaac taacgtcatt accaacaaat aatttcatgg aataaaaacc
    15301 tattttcgat ataaattcta gaaaatgctt cgatactgtg ggattccaca gaaactcact
    15361 gtgtggtcac gacctcgcct aatagaatac ggtaataaaa gagctaatga gttgatagta
    15421 aacacagcgc tttggataac agacttatga aggtagacaa agctacagga ctgattagca
    15481 aattctcgtt gaatcctttc catacatcca ttgtactctg agaaatcaca caataacgaa
    15541 ataaattccg gaaggaatat gttattcaaa aaaatttgtg ttttatagat actttaaaag
    15601 aaacttatta ggcaaaatat attttacgaa gactgatgag ttgtgcaaaa tcaaatctga
    15661 gtcagaatga acttcattct agctcagatt tttgtttaat gacttcagtc agtttcttgc
    15721 attacgtgaa tgaaaatggg actggtgatg tccaaaccct aaaaagttag accaaaatct
    15781 aacttcaggg gggatggtat ttttatggga aaatatagct ttgaattcaa gtcaacaatc
    15841 gttcaagagt atcttgaagg agaaggtgga ttaggatatt tagcaaaaat acaaggagtg
    15901 agatcaaaaa aaggtacacg gatggatcaa tcaaaagtta gaccaaaaaa tctaactttt
    15961 gggggtcgtt atttgtatgg caaaatatcc ttttgaatta aaactaaaaa tcgttaaaga
    16021 atatcttagt ggcgtgggcg aatatcggca tcttgccaac aaatacggta tttcaagcaa
    16081 gtctcaagta agtgattggg tgaatgccta tcgtgaatac ggagaggaag gtctattgaa
    16141 aaagcatcaa aatcagaaat attctgttca atttaagtta aatgcgctag agttatatca
    16201 aacaagtgag atgtcctatc gagaagttgc cagcacttta gaaatgaaca accctgcgct
    16261 tattgccaat tggatgcgta atttcagaga agcaggtatt gaggggctct caaaagagaa
    16321 agggcgacca cttactttga ctagaaaaaa agaaaacaaa aaagaaaata caacaactga
    16381 agaacgcgag cgtatcaagg cattagaaaa gcaagtccga tcccttcaaa ttgagaatgc
    16441 ctttttaaaa gagttgagga agctgcgaaa acaggaagcc cagcaaagac gcaagaatca
    16501 atcgcacgaa tcatcgccag cctccgagga tcattcaaac tagttgagct tcttgaaaca
    16561 ttgaaatttc ctaaagcaac ctatatgtat tggcagaaac ggtttgacag accaaaccca
    16621 gaccaagaaa ttgaagaaaa aatgcttgaa attcgcaagg agcataaaga ctatggctgt
    16681 ctgcgactaa aaaaagaatt agagaatcaa gggattcatg tcaataaaaa gaagattcaa
    16741 cgtctgataa aaaaactagg tatcgaagta aagtcttaca ctcgaaaatc tcgacggtac
    16801 aattcttatc atgggaaggt tggcactgtc gccaaaaatc gtattcatcg tcggttttat
    16861 acaaatatct gtcaccaaaa aattacaact gataccacag agtttaagta ttatgaagta
    16921 gacaccacag gtgtcgttcg tcagaaaaag ctgtatttag atcctttcat ggatttctac
    16981 aatagtgaaa ttctatctta tcggatttct gaaaaaccaa acgcactagc cataatggaa
    17041 ggattagaag aagcaatcca gatgacaaat gattgtccat ttcgtagaac cttccattca
    17101 gatcaaggat gggcctatca aatgaatgct tataagaata aattgaagca gcataaaatc
    17161 ttccagagta tgtctcgtaa agggaattgt ttagataact cacctatgga aaacttcttt
    17221 ggtttattaa agcaagagat ttttcatgga gaagtctatc ggagtttaga tgaattgaaa
    17281 acaaaaattg accaatatat ctattattac aaccataaac gcatcaaaaa gaaattaaat
    17341 tggcgaagtc cggttcagtt tcgagaagct gtaaaaacag ctgtctaaca gttaatttta
    17401 tcgtacaaaa aaggagtgga tttctccact ccataaaagt ctaacttttt ggggtcgcta
    17461 caaatgctta tcgagaattt ggagaagaag gcctcttgcg taagcgaaat aagcgaagtt
    17521 attctgttca attcaagata gatgcgatag agttataaaa aacgggtgag ctatcctatc
    17581 gtgaagtggc gaatcattta gggataaacc aacctgcgtt agttgtcaat tggatgaaaa
    17641 tttttgttca aaaggattgc acgggctctc aaaaatgaaa gggtcctcct ccattttgtc
    17701 taagaaagaa taaaaggaag aaacaagcaa actgacgcct aaagagcata atcgtatcaa
    17761 agaattggaa aaataagtgc gtttccttca gattgagaat acctttttaa aagaattgag
    17821 gaagcttaga aaacaggaag tctaacaaag acgaacgaat caatcgcacg aatcatcgac
    17881 aacctccgag gatcattcaa attagttgag cttcttgaaa ccttgaaatt tcctgaatta
    17941 acctttatgt attggcaaag acgatttgat cgaagaaatt cagatcaaga aatcgaagag
    18001 gaaatgctta aaataagaag tcaatataaa gattatggct atttacgtat gacaaaggaa
    18061 ttgtgggacc gtgggttcca tgttaacaag aaaaaagtac aacgtttgat tcgaaagctg
    18121 agtattgaag tacgatcatt tacgagaaaa tatcgccgct ataatactta tcgtggaaga
    18181 atcggcatga ttacaaaaaa tcgaattcat cgacgctttt acacaagcaa ctgtcatcaa
    18241 aaggttacaa cagatacgac agaactaaaa tattttgaaa tggataaata cggtgccaat
    18301 cgtgaaaaga agctgtatct tgatccattt ttagatatgt acaacagtga aattttattt
    18361 tatcgcattt ctgaaaagcc aagtgcgttg gctatcatgg aagctttaga ggaagctatc
    18421 tgcgcaacgg aatgtatcta tcgtcgaact tttcactcca aaaaggatgg acctaccaaa
    18481 tgaaagctta tagtcataag ttgaaagaac ataagatctt ccaaagtatg tcccgtaagg
    18541 agacctgctt agataattcg atcattgaga acttccttag tctattaaag caggaaattt
    18601 attatggaag agactatcgt atctttgaag aattgaagca agccattgat ggctatattc
    18661 attactacaa ccatgagcgt atgaaaagga aactaaactg gcagagtctg gtgcaattta
    18721 gagtaatagc attcaaagct gtctaaaatc atcaagaaaa gcaaagaaaa acggagtgaa
    18781 ttttctccac tccgaaaaca tctatctttt ttaggtcact acagaatcaa ttctgattca
    18841 tcagacccat aaattataga tcctaataat ttataccatg ctctttttac tcatatttac
    18901 atttatcttt gggtatttta tggtttaag
//
LOCUS       DACDCJ010000057        18534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDCJ010000057 DACDCJ010000000
VERSION     DACDCJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.057"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            234..551
                     /locus_tag="IV038_001933"
     CDS             234..551
                     /locus_tag="IV038_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8342134.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(642..1007)
                     /locus_tag="IV038_001934"
     CDS             complement(642..1007)
                     /locus_tag="IV038_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342135.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1215..1697
                     /locus_tag="IV038_001935"
     CDS             1215..1697
                     /locus_tag="IV038_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342136.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1894..2784
                     /gene="pstS"
                     /locus_tag="IV038_001936"
     CDS             1894..2784
                     /gene="pstS"
                     /locus_tag="IV038_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP8342137.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3071..4345)
                     /locus_tag="IV038_001937"
     CDS             complement(3071..4345)
                     /locus_tag="IV038_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8342138.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            4618..7089
                     /locus_tag="IV038_001938"
     CDS             4618..7089
                     /locus_tag="IV038_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8342139.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7192..8928)
                     /locus_tag="IV038_001939"
     CDS             complement(7192..8928)
                     /locus_tag="IV038_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP8342140.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(8925..9629)
                     /locus_tag="IV038_001940"
     CDS             complement(8925..9629)
                     /locus_tag="IV038_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8342141.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            9760..10263
                     /locus_tag="IV038_001941"
     CDS             9760..10263
                     /locus_tag="IV038_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP8342142.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10223..10561
                     /locus_tag="IV038_001942"
     CDS             10223..10561
                     /locus_tag="IV038_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP8342143.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10582..12000
                     /gene="ffh"
                     /locus_tag="IV038_001943"
     CDS             10582..12000
                     /gene="ffh"
                     /locus_tag="IV038_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP8342144.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12112..12690
                     /locus_tag="IV038_001944"
     CDS             12112..12690
                     /locus_tag="IV038_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP8342145.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            12694..13215
                     /locus_tag="IV038_001945"
     CDS             12694..13215
                     /locus_tag="IV038_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8342146.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13294..13848)
                     /locus_tag="IV038_001946"
     CDS             complement(13294..13848)
                     /locus_tag="IV038_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8342147.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14101..14376
                     /gene="rpsP"
                     /locus_tag="IV038_001947"
     CDS             14101..14376
                     /gene="rpsP"
                     /locus_tag="IV038_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP8342148.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14388..14639
                     /locus_tag="IV038_001948"
     CDS             14388..14639
                     /locus_tag="IV038_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP8342149.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            14764..15555
                     /locus_tag="IV038_001949"
     CDS             14764..15555
                     /locus_tag="IV038_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342150.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            15725..16246
                     /gene="rimM"
                     /locus_tag="IV038_001950"
     CDS             15725..16246
                     /gene="rimM"
                     /locus_tag="IV038_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP8342151.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16236..16997
                     /gene="trmD"
                     /locus_tag="IV038_001951"
     CDS             16236..16997
                     /gene="trmD"
                     /locus_tag="IV038_001951"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP8342152.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17133..17480
                     /gene="rplS"
                     /locus_tag="IV038_001952"
     CDS             17133..17480
                     /gene="rplS"
                     /locus_tag="IV038_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP8342153.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            17738..18031
                     /locus_tag="IV038_001953"
     CDS             17738..18031
                     /locus_tag="IV038_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP8342154.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         6154 a         3279 c         3751 g         5350 t
ORIGIN      
        1 aagataataa aatttgtatt tgtccttttt aggtaactta ttttaatcta tatacctttc
       61 tggatcaacg aaagtatact ttatatagtc ataacgccga tggtaactat gcactgtaac
      121 gataatgctg ctaaaatatc tttttacgaa aaacatcaca aggaaaaact taactatggt
      181 attatctaaa cataattttt tcggtattta atatttaatt aggaggtaat attttgcttt
      241 tttatagtaa ttttatctta atagtagcga tattattact tctgaatata tggatttttg
      301 ataggagtag aaattcaagt ataggtttta gaactaaacg aagtttgtct tctaaaaaaa
      361 attgggtata ctcacaaact attttttatg gaggaattgt tttaattagt ttactttctt
      421 caacacttta ttctttgaat attatagatg tttccacctc aaattcaata tctattattg
      481 gaatcattat tgctgcaatt attacacaac tgtttttagt gtttggagag aagaaaagaa
      541 gtaaaaaata gaaaaactag tgatagtttt ggtgatataa gaaaaacata tatgtataaa
      601 aagaagaaag tagcttctct ttgtacatat atggtttgtt tttatggatg ccgagctttt
      661 ttataagata aggctaccgc ccataacgac ccaaggattt gtggaactaa gtaaatacct
      721 gcaagaagca agatcagctg ggtcggtttt aaaggatgat agaagtaacg ccattcagta
      781 aaattggcgc aaaataaata ggaagtgaca gtagatataa tattggcaat gaataaagat
      841 ataatacgcc tgttcctttg aaaataatat ccagcaaaaa gagataaaaa tatcaatgtg
      901 atgaacccgc ttacggaatg ataacgaaaa tcaagcagta aggcgaaatg cacataagga
      961 acaaaagata aaagcaacgg aaggtttttc cagcaagttt ttatcatctt tcatcaccac
     1021 ctaacaagtg ttattatttt gttagtttta ttgtaaattg aatcctgcaa taatcaatta
     1081 ctttttcatt atttctagat taaatgaata aactctcatg ccattatctc taaaaaaaaa
     1141 gtttttcccc ctaatttttt tagatagaaa attgtatagt atgatgagag aaaaagtagt
     1201 ttgggggaac gttaatgaga agaaaagcag taatcgatcg gctttattta tttttttggt
     1261 tttttcctat cgttttgcta ccttttacat gggtaaatgc tgttgtacaa ggtgcggaga
     1321 tattaggcct gatccaagga agcggatttg tggtttctag gaattttcca gctttgattg
     1381 ttttctattt agtgataagt gtaggtctct tattaccaaa atctctgatc aatcactaca
     1441 attggcagct gctttacttt tcttgcttat tagtatttac atctatgttt tctttgctgc
     1501 ctcgcttgat tatcggccct aatggtttga gcgagagtgg attgaaggat ttagcttatt
     1561 cctccatgta tttcttgacg atcaaaccat ggttctattt agtaattgcc tcatttattc
     1621 ttgcaacatt tttttacttc tggacagaaa tcaggataaa aagtgaacga tctgtatcta
     1681 taaaaaataa ctattgatgg tggatcatca gtaaaaaaac atatgagctg cgtaaaatgc
     1741 atgtttctca tatgcttttt ttattattat gtaaagaatg cgaaaaacgt ttacatgatt
     1801 tttacataac ttaactatat ttttacagca accatacgag ggatttacag tcgactgata
     1861 aactttagtt gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt tactctaggt
     1921 atgatgggaa ttcttttagc cggatgcggc tcaggatcag gaaatacaga tggctctgcc
     1981 acacaaaata attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt aggttcgaca
     2041 gcattacagc cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa tccaaattat
     2101 caaatctctg tacaaggagg aggaagcgga actggtttga gccaagtcga agctggagca
     2161 gttactatcg gaaactctga tgtatttgca gaagaaagag acggagttga tgcttcaaaa
     2221 ttagtggatc ataaagtagc agtggtcggg atggcaccaa tcgtcaataa agatacagat
     2281 gtcaaagata tcaccaaaca ggaattgatt gatatcttta caggaaaaat cactaactgg
     2341 aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa cggcagcggt
     2401 acccgcgcga cattcgaaaa atggggactg gatggtgcta cccctgtgca gtcccaagaa
     2461 caagattcat caggaacagt tcggcagctg gtaagtcaaa caccaggagc aatcagctat
     2521 ttagctttct catatctcga tgattccaca caagcactaa gtatcgacgg tgtagaacca
     2581 aaagaagaga atgtagcaga taacagctgg ggaatctggt catatgaaca tatgtacacg
     2641 aacggaaaac cttcccctga agtccaaaaa ttcttagact acatgatgac agaagaaatc
     2701 caagaaggac ctgtcaaaga attgggctat ttaccaatca caatgatgga agtagaacgt
     2761 gatcacgaag gaaatatcaa ataactagca gataaacatg ccacggtagt cgttactatt
     2821 aaggaaaaat caactatgtt tccccacata tacatttttc ctttaattgt gaaaaatagt
     2881 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
     2941 ttcgaaaaaa agagctacga catagaactg tcgtaacttt ttttggctct tcgtcaaatc
     3001 gtgttggtga agacaaattt gaataaaact tagtccgata tgcggcgcgt tctgtgcgcc
     3061 gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt gcccgaaagt
     3121 tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg ttattcagcc
     3181 cctcagtgac gccgtttgag tgggacacct taaaggcatt tgaaataccc gattggtagc
     3241 aatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa attgttttaa
     3301 gggtttcttg aaagccttcc gtacctcgtt taacgaaata atagcgtaat aattgacaaa
     3361 aatgatacgc agttcgcaat tcttcgttgt agcttaacag ctcatcaacg atttctgttt
     3421 gactaattgg tcgtttaaac agtcgatgat aatgccgttt gagtggctcc catgtcgtgg
     3481 aatctttgag taagagtttc caatatcgct tgagccgacg gtacttcttc tgattttcag
     3541 agtgtgaggt tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag ctgcgattga
     3601 tgtgttggac aatatgaaag cggtcagtta cgatctccgc atttggaaag actgttttaa
     3661 gcaactgaca atagctggca ttcatgtcca tcactaggta cttgaccttt aaacgggcct
     3721 ttctaggaaa gcgcaggaag tattgagtta gtttgtaaag tctgcgatct tcaaggatcg
     3781 taaatagctg attggttgtc ccatcaacac aaataaaact catggaacca gaaacagaat
     3841 tcattgattt gaactcatcc atacacaaca ccgtcggaag gaagttcata cgtggctggt
     3901 aatgcttcgt tgcttcacgc aagatccgtg tcaccgtccc atccgagacg aagtacttct
     3961 tagcgattag tttacgtgat tgattttcgt acagttccat gagaatcgcc tgcttcagtt
     4021 cctttgttag ttgatgatgt tcatcaacta agtccgtttt cgcagaaaag gtccttccac
     4081 aatcatggca gagaaaacgg gtacgtttta attctaaaat agttaacttt tctttgactt
     4141 tcaacatctg agtgcgtgtc gtataggaac cgttgcggat aatacgaacc gatcgacagt
     4201 gttcacaata ggaagaagag tcatccaatt ttcctttaat aaactgcgct tgaacgcctt
     4261 tatattcttt ttcaatcagc cattcttcat caaaaaataa atgtttatct gttaatccga
     4321 gtaattttct agtatgatta tccattgaga gactcctttg gtaggatgac gttttggtcg
     4381 actaaatttt accaaaagtg agtctctttt tgagttgaaa cgaagaaaaa gtgttagtag
     4441 aaaatcgcct tcgattttcc accaacacaa aatattatag aacccttttt ttttgctata
     4501 tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat atctgttcta
     4561 tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg
     4621 aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa
     4681 gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt caaggaacga
     4741 atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa tcagcaaatc
     4801 gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc cattttgctt
     4861 tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc caatacagga
     4921 atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa tgttttaaat
     4981 acagcaaaag tcaatgtcat acgagacggg atagaacaaa caatagatat agaagacctt
     5041 gtaatagatg acattgttat tttagagact ggatatcaaa ttccagcaga tgcagaggtc
     5101 gtggaaggga aactgcaagt taatgaggct ttattgacag gcgaagcaga tgaaataacc
     5161 aaagaaatag gtgatccatt aatgtccggc agtttcattg tttccggaaa agcttacgcc
     5221 cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa agccaagaca
     5281 atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat caaatggatt
     5341 gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt ttttaatgga
     5401 aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat gatacctgaa
     5461 ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact agcaaagcaa
     5521 agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa tgtcctttgt
     5581 gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaaat gatcgtctct
     5641 aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc tgactatgca
     5701 aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt tacaaaaacc
     5761 actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt cagcagtgta
     5821 gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg cgatcaattg
     5881 ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt
     5941 ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac accattaggc
     6001 tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga ttacttcaaa
     6061 aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt atccaatgta
     6121 gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa attatcagaa
     6181 gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa
     6241 aagaaaatct tcgttcaatt gataaagcgt aacaatacag tagctatgac aggtgatggc
     6301 gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat
     6361 gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca
     6421 gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt
     6481 ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc
     6541 tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct
     6601 ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg
     6661 atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc
     6721 tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg
     6781 attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt
     6841 cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg
     6901 ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg
     6961 gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat
     7021 gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat atttaaacga
     7081 gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt
     7141 cgaagcagga tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt
     7201 cggaatggta acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc
     7261 attaaggagt tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg
     7321 tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat
     7381 gtcgatacct ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg
     7441 gataataatc tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat
     7501 aattggataa aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat
     7561 cgtcaaccgt ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg
     7621 ttctgtataa attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat
     7681 ttcccgaatc agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt
     7741 cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga
     7801 agtcacagga gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc
     7861 taaacgtcgc actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga
     7921 attagtaaag tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc
     7981 ctgatggatc agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc
     8041 ttgaatgact tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg
     8101 catctttgga ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat
     8161 catcagttcg ttcagtaacg tatataattg ctcttctgtg gtagtacacg cccgatacat
     8221 cttactcatt tgttcgctca acgcattaat cgtctgatac aattcttccc attgatcggg
     8281 tgtttgcatg attacttccg cctgattagg attttgcacc atttttttta gtacgggcag
     8341 cacggttttc aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac
     8401 aaggaagaaa gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa
     8461 aaaaacgctt gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac
     8521 tggtaatgcg acatataaaa gatcctcatt caaagtagta cttttgcgca aagcgctacc
     8581 taggttccca ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa
     8641 tgtttcattg tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc
     8701 gttcgactga tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc
     8761 gacatctaat tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc
     8821 cacgatctgc tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac
     8881 taaaagaata agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc
     8941 tgaaacttat agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc
     9001 tcgatttttt ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag
     9061 tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc
     9121 atgaaataaa caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg
     9181 gtcacttgat aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca
     9241 gcttcatctt ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct
     9301 aatacttcgc gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca
     9361 atgatccgat ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt
     9421 tcttgacgca gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa
     9481 atgatgaaat caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca
     9541 ctggttacct tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt
     9601 tcatcatcaa caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt
     9661 ttatatttta gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa
     9721 taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt
     9781 ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa
     9841 ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt
     9901 cctagagatc ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca
     9961 aaagtatgga cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa
    10021 gcctatcaaa aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct
    10081 tcatgcggaa cccattcgat ttatgacgga agtttttccg gaataaaaaa aaatggtgcc
    10141 ggagtggcta ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg
    10201 ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt
    10261 tgaattttat tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc
    10321 agatgatttt tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata
    10381 tgacaatatc aagcggacaa gcaagatctt ggaggattat gaaaaaaaac tccatctttt
    10441 ttctaattat attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa
    10501 gtacccggaa gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata
    10561 aataaaagga atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg
    10621 atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg
    10681 gaaatccgcc ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact
    10741 aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa
    10801 caaatcgtaa aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag
    10861 ttaaataaat caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt
    10921 aaaacgacct ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg
    10981 ctcctaatcg caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt
    11041 caacagctag acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc
    11101 cgtcaaggga tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca
    11161 ggacgtctgc atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg
    11221 caaccaaatg agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta
    11281 gctgatagct tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg
    11341 gatactcgag gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc
    11401 atcggttctg gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc
    11461 cgtatccttg gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactacgac
    11521 gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac
    11581 tttattgaac agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg
    11641 atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt
    11701 gaacgcaaaa aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg
    11761 ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg
    11821 aatcgaatga tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg
    11881 gatatgaaca tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc
    11941 aaaatggcga tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa
    12001 gatgaaaaaa acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag
    12061 caatttttat tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt
    12121 ccgtggagtg aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct
    12181 gaatatgatg accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg
    12241 agctggcaga cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat
    12301 atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga
    12361 attattcgta accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa
    12421 attcaagaag aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc
    12481 gttgattctt cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa
    12541 ttagcaaaag atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct
    12601 tttttagaag ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag
    12661 gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct
    12721 ataagtttag atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact
    12781 ccaacaggta agcaaactac ttatgaagct ttttatgaaa caatcgatac gaccatcatg
    12841 ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag
    12901 aaaaagaatt tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta
    12961 aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg
    13021 gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg
    13081 tatatccaga ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca
    13141 tatgaagcgc gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat
    13201 tatgtacgaa agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt
    13261 acatagttgc cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc
    13321 tagatacgca tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt
    13381 ttcatcaatt agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac
    13441 attcaaaaag acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc
    13501 tggtgatgca gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct
    13561 tgagcctaca tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt
    13621 aacgaaaacg acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg
    13681 agcctgttct ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa
    13741 tacactttcg tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta
    13801 agaatctttt cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct
    13861 ccttcaaact atctataata atcttacaaa agataagtaa ctggagcaaa taaaaacact
    13921 tactgacaaa agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa
    13981 tcttctattt tctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag
    14041 ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata
    14101 atggcagtta aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc
    14161 gtcgtagctg attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac
    14221 aatcctttga aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta
    14281 tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg
    14341 caaaaacacc acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa
    14401 gcgacttagt cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag
    14461 acgtcgtaga gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg
    14521 gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg
    14581 ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa
    14641 taaacgtgct gtactcgaat tttcgagtgc agcttttttg ttttttttat taattgaaga
    14701 aatgattatt tggcaaaaca agcaagaaag cgtatcctta taggaggagg gagtgaacag
    14761 ccaatgagaa agaaaatgtt atttattcca tttgctctat tgttaagcgc ttgtagttcg
    14821 gttactcaag aatcagacaa tacacatcta tcaagcagta tatctgaaac tgttaccagt
    14881 ccagaaaaaa gtagtaccaa aacggcttcc accactgaac aaacaatggc tacctcaaat
    14941 aatgagaaaa agactgcttt agaccagttg aaagaacaac agccaaatgt tcccatgcct
    15001 ctagatgtac cagtctcttc aggctatctc aatatcgcag cgacacatac aaagcaaggt
    15061 tattctattt tatattacag aacagatcgt cccctcgggt tgaatgctga cgaattgaat
    15121 caagaaacgc ctattgcaac ctatctatat caatatgggt ttgcttctag tcaagaaaca
    15181 atccaagttt tgcagccatt tgagatcgac acaaatggtc agcaagtgga cttaggttct
    15241 cgaatcacag ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga
    15301 aactggcgta ttcgaatccg tgggaataat atagaaggcc aagatccctt actattagct
    15361 aaagaaatcg tagcttattt ggaagaaaac agtctaccag cacctgaaca atttggtaaa
    15421 attactgttg atatgggtga tacgacgaat cgagccgtag aagtaagctg gcaagaaccg
    15481 aaaaatgcct atacgatcac tcatcaagat cctatgtctg cgttgaagat ggcagtttct
    15541 atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat catttaactc caagtcacag
    15601 gaagtaatca aaaatagcac gccgcatttt tgcataattt tggcttaata ccttaaaaga
    15661 aaactagaat tgcctcttca cacatgctaa aataaaacag acaaaaaaga aatggggata
    15721 taagttgact gaatacttga atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga
    15781 agtccgtgtt atctcaacaa cggatttccc agaagaacgt tacaaaaaag gaacggtgct
    15841 aacactattc caagacggaa aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca
    15901 taaaaatttt gacttattga gttttgaagg acatccttct atcaatgatg tggaaaaata
    15961 tcgggatggc atattaaaag tgtcaaaaga caactctgta gatctagctg aaaatgaatt
    16021 ctattaccat gagatcatcg gtctgcatgt attagaagat ggaactgagt taggtaaagt
    16081 aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc
    16141 tgatatattg cttccatata tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt
    16201 ggtccgtgta gaaattccag aaggactgat tgacgatgaa gattgatgta ttgactttat
    16261 ttccaagaat gtttgaaggt cctatggggg aatccatcat cggtaaggct gtagataaag
    16321 gattattaga tatcaatatc tctaacttcc gcgattattc agataacaaa catcagacag
    16381 tagatgatta tccatacggc ggtggagcag gtatgctgct caaagtccag cccatctatg
    16441 ataacatcca agccattgaa aaagctgcac cggatgtcaa aaaaagagta attcttcttg
    16501 acccagctgg taaacgattc gatcagaaaa tggcagaaga attttcacaa gaagaacact
    16561 tagtattcat ttgtggtcat tatgagggat atgatgaacg tatccgcaca ttagtgacag
    16621 atgaagtttc cttaggagat tatgtcctta ctgggggaga attaggtgcg atggtcatga
    16681 tcgatgcaac agttcggctt ttgccagatg tgttagggaa tcagacttca gctcaaacgg
    16741 attcttattc aaccggttta ttggaacatc cccaatatac tcgcccggca gaatataaag
    16801 ggatgaaagt accagaagta ttgacaaatg gaaatcataa attgattgaa gaatggcaat
    16861 taaaagaatc actaagaagg acctatcaac gtcggcctga tcttttggaa acacttgaat
    16921 ggacacctca aatgacgaaa tttttagaag aaatcaagaa agaagaacaa gaaaaaacag
    16981 cagatgaagc caagtgactt tttctaaaat cttctttaca gtaggttgat aatatgttag
    17041 tattttatgg tgagtgcaaa agcacttaac tattacgata ttccgctgtg agaaaagctc
    17101 ataagaatat ttggaataag gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa
    17161 aagaacaatt acgttctgac atcccagcgt tccgccctgg tgacactgtg cgtgttcacg
    17221 caaaagttgt tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac
    17281 gccgcggtgc tggaatcagc gaaacttata cagtacgtaa agtttctaac ggagtgggcg
    17341 ttgaacgtac attcccattg catacaccac gtgttgcaaa aatcgaagta gttcgctacg
    17401 gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa gctgctcgta
    17461 tcaaagaaat ccgtcgttaa tcattgttat cagactgaga aagaaccctt ggtattcaag
    17521 ggttcttttt tcttagcatg taatttgaac accctgatcc agttttttct ttaattattg
    17581 gtaggggaga ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca
    17641 ctatgataat atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca
    17701 taaatggagt attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca
    17761 agaaaagatg aaagttttag acacattcat gaatgatgga aaagtaaaac aagtaccaag
    17821 ttcaacaaaa aagaaataca tcctattgaa agaagtcttg aaacgctttg accatggagt
    17881 gacttataca gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta
    17941 tgtcgaacaa agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg
    18001 ggcatatcag atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag
    18061 ttcaagtatg cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct
    18121 ataatacaaa gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc
    18181 gattcgcagt taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat
    18241 caattggatg cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa
    18301 aacgtacctc ctaaaattag attttggaca gatgcccttt aaaaagataa caagatttcg
    18361 ttcttgaaac aatatatatg cttttattcc aatgaggttg tgaaaacagt attttgtcct
    18421 gagcgttaga aaattgaaaa tggggaatag caactcgtac tttaccattt tttaagtgaa
    18481 tacaagatca gctaattgaa caccgtttat tcgtgatgag ggacacaaca ctgc
//
LOCUS       DACDCJ010000058        18410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDCJ010000058 DACDCJ010000000
VERSION     DACDCJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.058"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..552
                     /locus_tag="IV038_001954"
     CDS             <1..552
                     /locus_tag="IV038_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8342155.1"
                     /translation="NAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSH
                     PEMGHMLIKRHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEA
                     FYLAQCAYNTTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVT
                     PELGDSAGIIGGLALARKAIRNK"
     gene            complement(2043..2708)
                     /locus_tag="IV038_001955"
     CDS             complement(2043..2708)
                     /locus_tag="IV038_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8342156.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2834..4870
                     /locus_tag="IV038_001956"
     CDS             2834..4870
                     /locus_tag="IV038_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP8342157.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4874..5512
                     /locus_tag="IV038_001957"
     CDS             4874..5512
                     /locus_tag="IV038_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8342158.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5540..5968
                     /locus_tag="IV038_001958"
     CDS             5540..5968
                     /locus_tag="IV038_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342159.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6425..7336
                     /locus_tag="IV038_001959"
     CDS             6425..7336
                     /locus_tag="IV038_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8342160.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7553..7990
                     /locus_tag="IV038_001960"
     CDS             7553..7990
                     /locus_tag="IV038_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342161.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            8085..8969
                     /locus_tag="IV038_001961"
     CDS             8085..8969
                     /locus_tag="IV038_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP8342162.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            9193..12333
                     /locus_tag="IV038_001962"
     CDS             9193..12333
                     /locus_tag="IV038_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342163.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            12380..12925
                     /locus_tag="IV038_001963"
     CDS             12380..12925
                     /locus_tag="IV038_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP8342164.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12953..13408
                     /locus_tag="IV038_001964"
     CDS             12953..13408
                     /locus_tag="IV038_001964"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP8342165.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            13405..14163
                     /locus_tag="IV038_001965"
     CDS             13405..14163
                     /locus_tag="IV038_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP8342166.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(14249..14989)
                     /locus_tag="IV038_001966"
     CDS             complement(14249..14989)
                     /locus_tag="IV038_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8342167.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            15050..16303
                     /locus_tag="IV038_001967"
     CDS             15050..16303
                     /locus_tag="IV038_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP8342168.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16625..16852
                     /locus_tag="IV038_001968"
     CDS             16625..16852
                     /locus_tag="IV038_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP8342169.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16975..17301
                     /locus_tag="IV038_001969"
     CDS             16975..17301
                     /locus_tag="IV038_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP8342170.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            17369..18082
                     /locus_tag="IV038_001970"
     CDS             17369..18082
                     /locus_tag="IV038_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8342171.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT         6272 a         2966 c         3694 g         5478 t
ORIGIN      
        1 aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca
       61 tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa
      121 gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt
      181 tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt
      241 gaaaaacgat tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt
      301 gaagcctttt accttgccca atgtgcatac aatacaacac tgatgctttc gccagataga
      361 attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa
      421 tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgctagcta cattgttacg
      481 cccgaattag gtgatagtgc tggaataatc ggcggattgg cattggctag aaaggcgata
      541 agaaataagt aattttaatg aataaatgtt tcattggtac tcatgtcaaa agtatttaac
      601 tgagtaatca aatgatgttt gataaagcga aaatgaattc aaataaaaaa ggatcgagca
      661 aactggaaca tttgtccgat cctttttcac attctattta tttcgtttat tcaaccaatc
      721 ggtagagaca tccattgcgc tcttgtcgcg tggctttgat ccatataatt gaccattacg
      781 aaatcatgga tcatcgcttt gaatgcgaac ttgtgtcaca tctacttttt tgaccagatt
      841 ttgaccagtc tattttacat tctaatgtac ttatatgagt taattttttg agtaaatggt
      901 aagtttttcc ttgtttgttt tatcttaaac tagtgtgtaa tttgaaaatc tatatggaag
      961 ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag gttcctagcc
     1021 tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt atcagctata
     1081 taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg tagctctgct
     1141 tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga agataagata
     1201 actgtgttat ataaactagg taactaactg taacaagcta attttaagag attcttatta
     1261 gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca tttcaataat
     1321 gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg gaaaaaagtt
     1381 cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca agagataaaa
     1441 cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa tcccatagat
     1501 taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa agtctcttcc
     1561 tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa caatccattt
     1621 tgtattttat tgagtactct tggataaact accagcgatt atcatattta tttatgttac
     1681 tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa gaactaaaat
     1741 tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact agattggtcg
     1801 ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct tgattatgat
     1861 tccaaaaaga atcattggaa ggctgtggcg taaatattat actataagcc agaaatacct
     1921 atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa ttaatttgta
     1981 cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg ttcatctcct
     2041 acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata gtcttggctg
     2101 aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac gttcatgacg
     2161 tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc tccagcgtga
     2221 aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc gatggaattg
     2281 tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa aggattgaat
     2341 cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac caaacaaaag
     2401 acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga tgaataagat
     2461 ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt agctgattca
     2521 atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag catttcaaag
     2581 tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac accaattttt
     2641 tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat tttttctccg
     2701 gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac atgacctata
     2761 cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag aatcatttaa
     2821 gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa tcatagtgac
     2881 cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct atcggacaaa
     2941 tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac attttattgg
     3001 gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag aaactgagct
     3061 ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt ggaacttcaa
     3121 gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat cttctcacga
     3181 gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat cgatcaaaat
     3241 aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct atgaacgggt
     3301 catttcacta ttcactgatc gattcaatca agcatttcac actgattatg ccactaatat
     3361 ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag acgtatataa
     3421 ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat catccttttt
     3481 cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat tacatcaagt
     3541 attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg caggaatcat
     3601 tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc agatgtacgt
     3661 tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag ttgaaaaaat
     3721 cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat ttggattgtt
     3781 gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt tttcttcttc
     3841 acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact atcatgtatt
     3901 ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc gttttgcatc
     3961 agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat tagtcaatgt
     4021 atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa taaatctgaa
     4081 ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa taaccgaaaa
     4141 agaaacgcac cctgttatat tgatccctga acgatataga catacagatt taaaaaatga
     4201 cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga aatttctatt
     4261 tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga tcgaacatta
     4321 tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa atattctcag
     4381 cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag aaaggttatt
     4441 tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc cgtatactca
     4501 gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga tacagatatt
     4561 tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca tatattttgt
     4621 ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct ttgaaacgta
     4681 taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa tcttcctttt
     4741 tcttatagaa agagagccta aatatctttt taatattttt ttctttagct tcattgattt
     4801 tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat tgctgatgaa
     4861 tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa tcattcgaaa
     4921 caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg tgactgttgt
     4981 tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga atgatcagtt
     5041 cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg ggagagagaa
     5101 gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt tgattgaata
     5161 tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa atgtttcaga
     5221 taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga agctaagtac
     5281 gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga gagtcttatt
     5341 gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata aaagaaataa
     5401 ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag ttatcgtagt
     5461 cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt aaaaatcaaa
     5521 aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag ggataggttt
     5581 tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg gtgaaatcta
     5641 ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata ttattaagtc
     5701 aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct ctggaagatg
     5761 ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc actatagtcg
     5821 tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat cagcaaatgc
     5881 aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca cattctcatg
     5941 ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa tagaaaaaac
     6001 atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca tttttagttg
     6061 tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt
     6121 gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac atttgttcta
     6181 agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta acaaaagaag
     6241 tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa ctttctggat
     6301 tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg ttcttgatga
     6361 ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt aggagaattg
     6421 ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct tattgtcagg
     6481 ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa tcagcgaaca
     6541 agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc aacagttgat
     6601 cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag aaatcgattc
     6661 tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat tgaaatctgc
     6721 aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct ttcaagaagg
     6781 tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg aatctttcca
     6841 tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa
     6901 gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt caactgatac
     6961 tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa cggttccggc
     7021 agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg tgatcacttc
     7081 taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa
     7141 caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa
     7201 attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag acgctaatgt
     7261 aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag attctagtac
     7321 agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc aataaaactg
     7381 ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta cttgatttag
     7441 ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat atttttctgt
     7501 ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat aaatggagtg
     7561 gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg tttttctcac
     7621 aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc atcaattttc
     7681 cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta ttcaaggccg
     7741 tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc ctctgatcgc
     7801 tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc tatttttgtg
     7861 tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat cattttcttt
     7921 tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt tttatcttgt
     7981 caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt ttttctttat
     8041 gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa aaagttttat
     8101 tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa ttagcgatag
     8161 caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg
     8221 caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt gtcatgggcg
     8281 gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat cggccaaatt
     8341 tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg ataccgctag
     8401 aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg gatattggaa
     8461 aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct gaatcaatca
     8521 atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc atgtcaggaa
     8581 tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat ggaaagaagg
     8641 aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc ggttttgaaa
     8701 cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat ttaaaagatt
     8761 cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta gatgaatcta
     8821 cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct gatgaggaag
     8881 aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca atcaaagtat
     8941 tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga gttggcggga
     9001 gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc cttttttttg
     9061 tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca tattttaaat
     9121 atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa acgtcagaga
     9181 ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt gtttgtcttc
     9241 cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga atcaacgcaa
     9301 tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac ttctgaaact
     9361 acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa gacaagtgag
     9421 acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc aagagctgtt
     9481 tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct tctcgcagac
     9541 gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga attagagatc
     9601 aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac acaccatatt
     9661 tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac agctgcacat
     9721 ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta tatgaccgat
     9781 ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac acttgatttg
     9841 tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac attagatggt
     9901 gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc tccgggtaaa
     9961 gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact ttatggaccg
    10021 tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag tggaaaaaca
    10081 gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga tcgggcagat
    10141 ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat gtttgaagtc
    10201 agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa tgacaacaat
    10261 cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa tggtgggaaa
    10321 atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc tagtcaaatc
    10381 catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga tcaacccggg
    10441 aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg agccggatca
    10501 gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga gaatggtacg
    10561 caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg tgatggcaaa
    10621 tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga cgcttctggt
    10681 tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc aaatatcgat
    10741 atacgcgcag attccggagc aactgtagat acaactggag atattgattt aaattttacg
    10801 gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa gaccggcatc
    10861 atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga tttccaaaac
    10921 aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg aaatggagat
    10981 tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg cgcaacattc
    11041 gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc gatcaatggg
    11101 accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga atttgctcag
    11161 acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct cccgatgatt
    11221 gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt cgttgttccc
    11281 gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt gactttacgt
    11341 attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac gcaaggacaa
    11401 acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca aggcggcttg
    11461 ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt gcaagttgtt
    11521 gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc caaaacgaca
    11581 gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa tgcagcttct
    11641 cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa aaatcaagct
    11701 gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat cccagaaaat
    11761 caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg agaagctact
    11821 gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca caatccaagt
    11881 gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa gatcgtagta
    11941 gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat caaagcaact
    12001 ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg gatttatgat
    12061 cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc tttcacaaat
    12121 gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga gtatcaatta
    12181 gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt gcaagcagat
    12241 tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa agaaaatcag
    12301 atagctggta attactcagc agagataaca tagacattag cagatgcgcc gacagaataa
    12361 agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa acaactgctc
    12421 tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc atgcctccga
    12481 attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga acaaaacata
    12541 tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg tctttaaccg
    12601 aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga attcaaacgg
    12661 tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt atgatatgaa
    12721 aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg tcactgagac
    12781 actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag ggatcacgtt
    12841 tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca atcagttttc
    12901 tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag atttgaaatt
    12961 aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga atcttcaaaa
    13021 tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca aaaaagaaac
    13081 aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca atacaaattt
    13141 tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc aagcggttgt
    13201 gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa taactgatga
    13261 tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag aaacatttga
    13321 ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg gattatttat
    13381 tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac cgttctagct
    13441 ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc agccactaca
    13501 gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt tgttccagtt
    13561 cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc aaatcaggga
    13621 acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac aatcaagtta
    13681 ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac tggagcacaa
    13741 acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc cggttggaaa
    13801 ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa actaactggt
    13861 tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag cagtggaatg
    13921 ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta
    13981 gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc attaggagag
    14041 aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa ttatgctgta
    14101 gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga agaggaagcg
    14161 taaaatcata tcaacgactt cagggagtgg agccgattca aggcccactc cctgaaatct
    14221 gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg aagtcatgga
    14281 aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca ccgttgaaaa
    14341 attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg atacagtaag
    14401 attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt tttaaagaag
    14461 tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc aaacctttga
    14521 tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct ttttgatcaa
    14581 taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt tctagcactt
    14641 gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga aggattttcc
    14701 gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata ttactatgtg
    14761 catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc ttcaagtcga
    14821 tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac tgattgattt
    14881 gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat tctctgatga
    14941 tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata tgcaggctcc
    15001 tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga tggtcgcaac
    15061 agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt tattcttttc
    15121 gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg tgacactttt
    15181 acttcctatc cctaaagtga gctttcctta cctaattggc ttattattga tgtttctttt
    15241 ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt tttttgctat
    15301 cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa agagacatct
    15361 tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat tagtaaaaag
    15421 tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg aacactattt
    15481 tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg gaagtgttca
    15541 cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca gttttttttc
    15601 tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac atcaggaatc
    15661 gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat tcaaacatga
    15721 ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa aagaaacgtt
    15781 ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac cgcaaataca
    15841 acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat ttctagaaaa
    15901 agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga aaacaatccc
    15961 gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg cattcgatca
    16021 tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag agggatcacg
    16081 ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga tggtcaaaag
    16141 aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa aaatgttgga
    16201 tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact atttttttgt
    16261 agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg tcaaaggata
    16321 agtttgagga aagtctacac ggagatagag acaacaacac agcatattca aaagaaacag
    16381 aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa caaagtagtt
    16441 gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa gtagataaga
    16501 agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca ccagataaag
    16561 ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta cgatcacaaa
    16621 aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc atcgaaaagc
    16681 ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa aatcattaag
    16741 agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac aaaaagcctc
    16801 aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt gattttctat
    16861 gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc tattttttat
    16921 gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa gcaaatgaat
    16981 agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt tcataacgaa
    17041 aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact ttttcaatgg
    17101 aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga actggcagat
    17161 gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga tgagatcatt
    17221 gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc aggtgtacga
    17281 aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc aaaagaaaaa
    17341 cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa aaagatggct
    17401 agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt tcaatgaaca
    17461 gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga atgtaacaaa
    17521 agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg cggtagaacc
    17581 aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac cagtgattgc
    17641 tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct tggataatac
    17701 gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa aaggaaatta
    17761 tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc tagaaaattt
    17821 agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat acaaaacatt
    17881 ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg agggaaaaga
    17941 aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag ttgtacaagg
    18001 agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat ctgctttcaa
    18061 tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt aaaaacagcc
    18121 aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa cgcaaagagg
    18181 tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt tattttttat
    18241 atgaaaaggt ttgcataaat aaaaattcga tggtatgatg attttgaata agaaatggat
    18301 tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg aattctaggt
    18361 ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt
//
LOCUS       DACDCJ010000059        18228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDCJ010000059 DACDCJ010000000
VERSION     DACDCJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.059"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            388..987
                     /locus_tag="IV038_001971"
     CDS             388..987
                     /locus_tag="IV038_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342172.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            1099..1323
                     /locus_tag="IV038_001972"
     CDS             1099..1323
                     /locus_tag="IV038_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342173.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            1675..1911
                     /locus_tag="IV038_001973"
     CDS             1675..1911
                     /locus_tag="IV038_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342174.1"
                     /translation="MLSDYKEELKLIENRVLGFVECKDNLDDTLEVLESIIFNTKVLD
                     LKAINGDMTTHIILDEISAKEIGETLIAWANKEL"
     gene            1991..2845
                     /locus_tag="IV038_001974"
     CDS             1991..2845
                     /locus_tag="IV038_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8342175.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            2858..3295
                     /locus_tag="IV038_001975"
     CDS             2858..3295
                     /locus_tag="IV038_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342176.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            3615..4058
                     /locus_tag="IV038_001976"
     CDS             3615..4058
                     /locus_tag="IV038_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8342177.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            4203..4526
                     /locus_tag="IV038_001977"
     CDS             4203..4526
                     /locus_tag="IV038_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342178.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            4944..8573
                     /locus_tag="IV038_001978"
     CDS             4944..8573
                     /locus_tag="IV038_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342179.1"
                     /translation="MKKLKLISIALFSLITMSTARQSIVSAEGTSTKAVVEESGDKKV
                     AIDDKVKNEAEKATVVQGADGLMYVVVDENDPEKLLEAESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFESE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRHALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVVFTIYNSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEISQDTPFTKPAKVKVT
                     NIYEVSISEKVSDMNESQVLKNNWSADPNDYLQYDITTGNLKKSGERNVQSFIIEGFY
                     DNKNVDVSKSEVYVGSTNVTNSFDINNDTNNGKVIAKAKTSVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKNLTIVKNSKVTVGSKISKSASSNDVETVLYSRKVTINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDVIHKGKIDGKDIVLNTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTKIELSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMSLDLPTEYLTKNKKINYSVDILLVDNPDKNKFETETKDLRTIGYTSS
                     EKVLTNKDMTNNQVNYSAVVRTVKERENNNPVKEFNESINYKFTDSTKTKTGYGISVD
                     LEPTYRNEIGKQIDMKVYSYPMNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGI
                     STQNLSFQFPHMNIERKTGNIYADSQRNDESIKYELIDGGRKLYVPIWCDLGNYSIAI
                     KSSNFGGNYISLDMNQNIDVYAYMYATIDSETKKDDELLLEPVYPESTLPDGWSKNEI
                     DWLNK"
     gene            8633..8995
                     /locus_tag="IV038_001979"
     CDS             8633..8995
                     /locus_tag="IV038_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342180.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            9229..9651
                     /locus_tag="IV038_001980"
     CDS             9229..9651
                     /locus_tag="IV038_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342181.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            9663..11078
                     /gene="cpaF"
                     /locus_tag="IV038_001981"
     CDS             9663..11078
                     /gene="cpaF"
                     /locus_tag="IV038_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP8342182.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            11095..11910
                     /locus_tag="IV038_001982"
     CDS             11095..11910
                     /locus_tag="IV038_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342183.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            11910..12707
                     /locus_tag="IV038_001983"
     CDS             11910..12707
                     /locus_tag="IV038_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342184.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            12725..13087
                     /locus_tag="IV038_001984"
     CDS             12725..13087
                     /locus_tag="IV038_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HAP8342185.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            13259..13480
                     /locus_tag="IV038_001985"
     CDS             13259..13480
                     /locus_tag="IV038_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342186.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            13499..13774
                     /locus_tag="IV038_001986"
     CDS             13499..13774
                     /locus_tag="IV038_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HAP8342187.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            13883..15709
                     /locus_tag="IV038_001987"
     CDS             13883..15709
                     /locus_tag="IV038_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342188.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            15722..16438
                     /locus_tag="IV038_001988"
     CDS             15722..16438
                     /locus_tag="IV038_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342189.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKR
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            16451..>18228
                     /locus_tag="IV038_001989"
     CDS             16451..>18228
                     /locus_tag="IV038_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8342190.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHF"
BASE COUNT         6935 a         2262 c         3475 g         5556 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac tcttattttt attcaaaagt
      121 gcccaaaata taggtatact gaattaagga taaatacaaa actagttaga ggacagttga
      181 aaaaatacaa acaaattaga ttgctatttt gttaaattgg aagtagaata ataccgtttt
      241 gatatgtata gtgtgtttta ccaataaaat tacataaacg tgaatatatt tttatgaatt
      301 aaatgtaaaa tgagagcaat tagttttaaa aaagattata ggctgttaat cttagcatat
      361 ataatccatt ggaaaggggg agatccaatg aatctaaaga atacaaactc aaacaaatct
      421 acactatatg taatttcaga tgattatata aataagctga aaaaggtgga ctatcgtgtc
      481 caaaacaact atcgagaaca acgattatat tttaaatcaa acttgaaaat ttctaacaaa
      541 aatgaagaaa taaattatta tgtaccattg gcttcagcaa aagaaaatca acgttctttg
      601 actaataaat cagtgtttaa gatttatggt gatcgagaag aaaaggactt tttaggagta
      661 cttcatataa ataatatgat tcctgtgcca aaagattgtg caaaagtatg gacacctaat
      721 agcccaaatg ttgatgagaa gtataagatg ttagtaatca aacaggcaaa atatttgaaa
      781 ttacatgagt ctgaaataaa tgaatcatgc gaattacttt ataattctaa gttaggtaaa
      841 cttgatccta gttactttaa gaaaaatcgt aaagaagtaa ttacttatca aaaaataatg
      901 acgaatgtag aatcattgga agctgttagt atagcagaat cccaaagaaa tattcatgta
      961 caaactcaaa gtatagatgt agagtaagat aggctaaacc tatcttattt tattattcta
     1021 ttggatatta tgatacattg aattttaatc gaatatgtat ttaaatgtta ttatactgta
     1081 gaagataaaa cttaaaggat gatttctatg gcgagaccga gaaaatacaa tacagaagaa
     1141 gaaagaaaag aagcaatgaa aaaggctagg aaaaaatggg ttgaaaaaaa tcaggaaact
     1201 attgcctata taagagccaa atcatttgct aaaaaattca ttgaaatgtc tgaagatgaa
     1261 gatattgaat tgttggaaaa atggttagaa gatcggaaaa ataacggtca aaatgatgaa
     1321 taataaccat tgactacaag tagtcaatgg tgtagaatgg ttttagggat agaaatatcc
     1381 cttttctaaa agtctaatga ctacaagtag tcgatagagg gatcgggaat aaaaacagac
     1441 gattatggcc taaagaaagg aggaatatga atattttgag gaacgattta ttaaatgagc
     1501 gttgataaat ttcactaaat attttcttaa tatctatgta ttgttcaagc gtagaaaaaa
     1561 atattgaaat tattacaaaa ccgctcataa tggaaaataa cgaattatat taatgaatcg
     1621 ttcacttttg acctttttta ctagattttt ttgtaggact tttagagaat ttttttgtta
     1681 tctgattata aggaggagct aaagttgata gagaatagag tactggggtt cgtagagtgt
     1741 aaagataatt tagacgatac tttagaagtc cttgaaagta ttatatttaa caccaaagtt
     1801 ctggatttga aagcaattaa tggtgatatg acaacacata tcatattaga tgaaattagc
     1861 gcaaaagaga ttggcgaaac tttaatagca tgggctaata aagagctgta aaagataatt
     1921 ttaaaataaa gtggccaatt gactacaagt agtctgtata gtataataaa ttatcaaaag
     1981 tgaggtatac atgatgaaat caaatttttt gggatatgta attgcaatca acatgtttaa
     2041 agggggagtt ggtaagtcaa ctcttactca gctaatggct gatattctat cggctagagg
     2101 ctttaaagtt ttagttattg atgttgatcc acaaggaaca gtaactaaaa agtttcaaag
     2161 acaatatgat ttaaaagttg ttgggaaaat gacattgttt gaagcagtaa ttaatatgtc
     2221 tttcttagac tgcatttctc ctgttaaaga aaacttagat attgtgcttg gttcagaaca
     2281 aatgtcaagg tttaccaaag tattaagaga tatgtggaga agtactggtt ttactgatga
     2341 aggtgaagaa gtcttttgga atttgtttaa ttatatgatc ggtaatgata atttaagaga
     2401 tcaatatgat tttattttgt ttgacactat tcctacagtt tcagaattta cagataacgt
     2461 ttacgtggcg agtgattttt tagtaattcc cacgcaaact gagcaagatt ctatagacaa
     2521 cttgcatagt acggttacta attatcaaac tgctgtaaaa gaagtaaacg gagatttaga
     2581 aattttaggt attgttccat atctagtgga tactaaatta aaaacaggat tagaactttt
     2641 atcagaactt caagaagaat atggagactt agtttattct catattgttt cagaacgagg
     2701 cgtaattaaa cgttggggta gagaaggact aaataaatct atcccacatt ctaaaactgc
     2761 ttatagcatg tatgtagaca ttgtagaaga aacgttagta cgtttagaaa aagctacagg
     2821 agaaaatatt ttagaacgtg tttaggaaag gtgttgcatg atgtcgaaaa ctaaaaaagt
     2881 aactagtgcc ggcagtaatg gttcgggtaa tccgttcgga ataaaaagcg gtagtggtgg
     2941 atttaatata ggacaacaaa tgttaaaagg taaagctggc attaagtcta ctggtgataa
     3001 acggaaatct gttagggtac cagttgaatt atacgatgaa gttgttaaag atttatcata
     3061 cggggctaaa tattcattaa tgacagaaat tttagcttta tttttaaaag aatttgaaac
     3121 acatggaaat agtatagtta ctgattttgg caagttgaaa acgagaccag taaaaatttc
     3181 tgaagaagcc tttaaagttt tgaaagttat aaaatttgaa acagatcaat ctaatactaa
     3241 tattattgct tcagctttga catggtatct tgataaaaga aaaggaaaag cataatgaaa
     3301 tgaggtaaga ctatggatca taaatcaaat tattcataca aagaaaatta tatttggaag
     3361 tatgtagtag ccgtttttct actggtaggt ggaattattg cattggttaa atgggctata
     3421 attcctaatg ctttagtatt tatcaattgg tttgtcccta atttttggag tttttttggc
     3481 tatatattga taatctggtt tatgtttaaa gtactctctt ttttctggtc gcctgtatat
     3541 tctttgcttg gaaagtggtc gtacctaatt ttaatagcga cagctattta tatcattatg
     3601 cagattaact aattatgcag attttagaag atattaaaat ttattggaaa aggataatag
     3661 gctatacatt agttcttctt gtagtaaatt tattcctttc gatgatagcg ttggtctttg
     3721 ttaatcaaag aaaagacaat gtaactgaat tagtaacgtt tatttcgttt ttcataagtg
     3781 ggatttttgc tcctttcttt gaagaaacct taatgagagg aattatccaa aaccaattgg
     3841 aattaaaaac aaatttgtct acaagaagca tatatataat tgttgcaatt ttgtttagtc
     3901 tatcccactt aaatttatat tttattcctt attttatgac gagtttaatt ttgtcttttg
     3961 tctatgataa ttcagataaa aggctgattg ttccggttac tattcattgt ttttacaatt
     4021 taattgtaat actgttaagt gtagtttttt atacttgaaa aaaatagacg aaaacctaaa
     4081 aatacaaggt ttttatctat tttttttatt ttgtttttat ctatcaggtt gatagaaatt
     4141 gtcataaatg atataattaa gtatgaactg atttagtaaa aaaattaaag gagtgatagc
     4201 tgttggcaag atcaagaatt gctaaaattg aacagactaa aaaggataaa aaaatttcta
     4261 aaaataaaac aaaattcttt aaagctgaag atgttaagag gatagcatgg tctgtgaaaa
     4321 catttgatga agatcgttat gttaaagttt caatgaatgg tctatttgta cagcttggta
     4381 acttcagtaa ttttaaaacg attgatgaac tttatccgga aataagaaat attgtggata
     4441 gtaaaggttt aatcgtggct aaacggaaat caagaaactc tggtcttgct tactgtaaaa
     4501 ataaagggat catgattccg gcttaaatca gtgaaattgt tctttgaaaa cttaatacta
     4561 ctcgtactgt agcaatgttg ctatgttaca gataaaagcg taaggctaat aagcaaaata
     4621 gcaaccaagc atactgttat ttcctttgcc aagaggtatg taaatacgtt agcaatagaa
     4681 gtgataaggt tagtatttca cgccaaaaaa tacaaaagtc gatccattga aactactaat
     4741 tatgaggtgg cgtaaatggt aattattcag aatttctatc taggaagaac ctatgaaaac
     4801 cgtcctagag atcagaatat tgacactaaa aaattcagca cagtaataaa tgtgctagat
     4861 acagggtaac tctttactac aagaaatgta gtaaagagtt gttatgattc tttattacat
     4921 ttctttctaa aggaagtgtg ataatgaaaa aactaaaact gattagcatt gctctattca
     4981 gcctaatcac tatgtcaacg gctcgccaat caattgtttc ggctgaagga acgtctacga
     5041 aagcagtagt agaagagtct ggcgataaaa aagttgctat agacgacaaa gtgaaaaacg
     5101 aagcggaaaa agcgactgtg gttcaagggg cagacggctt gatgtatgtg gtcgttgatg
     5161 aaaatgatcc tgaaaaattg ctagaagctg aatctccttt gaaaaatcaa caagaaaata
     5221 ccggcgctgt tgaagtcggt gttgatgaaa agagacaaga aaaggctgat aaagctaacg
     5281 aaaaagtgaa aaactttgac gatattgatt acaacgatat tagttcgttg cagtcagcgt
     5341 tgaatgatcc gaaattccct tttttatcgg aagatgaatt aaatgaaaaa ttgcaaaaga
     5401 caaagaatac atttgagtct gaagcgctaa tgaaaaagac aaggggatat ttcagtcaaa
     5461 ggattttaga gttaagcaat aatttaaatg acgtaccgaa aagagcgaaa gtgagtcggc
     5521 atgctttagt tagtaaccgt gcttcacaag taagtgaaac acgaataaac cctccaaatg
     5581 gttatccgtg ggtgatttat ttaattagga gcaatggtta tagttataca gaaaattatg
     5641 ctattagaag aactattgat gggaaagaag ctttttgttt acaggccggt gtacaagcga
     5701 cgaatacata cactggttca acaagtggtt atctttctaa ttcgcaaaga gaaatgatta
     5761 gttcgatttt atcagcatat aaagaattag gcggttgggc taataatgat gccggaaaac
     5821 aaagatggat agcaactcaa atgcttgttt gggaaaacag taatgaaaag cctaaaagta
     5881 taactgttac gccaaattgg aaaaacggag ctactaagtg gaaaaatgat ttagtggctc
     5941 gtgcaaatgc tattagagta agaacaaaat gggattctac gtctaagacg gttgatgagg
     6001 gagataaagc aacctttaaa gtaactaata cttatggaga tcataatttg catgtgaaat
     6061 ctgtatctgg tggcggcaaa gctaccataa gcaatggtac tttaactgtt gatactacag
     6121 gagccactgg aaacaaaatc actgtagata tgattaaagg ttctcaccct tcagacgcgc
     6181 accctacaac ggtatggagt aatggttctt atcaaaagct tgttaccggt tctttttaca
     6241 ttagtacttc tgttacggta aatgttaaga gaaaaggaac tgttgaagtt ttaaaagttg
     6301 attcagaaac aaaaaaacca ttatcaggag caacatttag atttagctac tcaggtaaaa
     6361 caaaagatgt tactactgat tcgaatggta aagcaaaatt aactgaaagg ttgaaagctg
     6421 gaactgtagt aaaagttaag gagataaaag ctcctaatgg ctaccaacta gattcaagcg
     6481 aattttcaat tacagttaaa gaaaatcaaa atgttactac cacaagaacc aataagaaat
     6541 ccacaggttc tgtagaaatt gaaaagatag gcgatttaga tggattagga ctaccgaatg
     6601 tagtattcac gatttataat tccgataaca aagttgtaaa agataactta aaaacagata
     6661 gtaatgggaa aataaaagtc gatgaactgc aatttggtaa atattatgca gtagaaaaac
     6721 aaggtgttac tggttatgat cccgatggta aaaaatacaa ttttgaaata agtcaagata
     6781 ctccttttac aaaaccggct aaagtgaaag taacaaatat ttatgaagta tccataagcg
     6841 aaaaagtatc ggacatgaat gaaagtcagg tattaaaaaa taattggtct gctgatccaa
     6901 acgattatct tcaatatgac attacaactg gaaatctaaa aaaatcggga gaaagaaacg
     6961 tccaaagttt cattatcgaa gggttttacg acaataagaa tgtagatgtg tcaaaaagtg
     7021 aagtatatgt tggatcaaca aatgtaacaa actcttttga cattaataac gatactaaca
     7081 atggaaaagt aattgctaag gcgaagacaa gcgtattaac gaataatgat ttttataata
     7141 agtcctataa tttacggatc actatgaaaa ttaaaaaatc tactcttgaa aacactgaaa
     7201 agcaaaaaaa tttaacgatt gttaaaaata gtaaagttac tgtcggttca aaaatctcta
     7261 aatctgctag ctctaatgat gtagaaaccg ttttatattc aagaaaagtt actatcaatc
     7321 atattgatga aaaagacaaa caccttttga aacaagatat tgattataaa tacgatgggg
     7381 aaacgtatga atataaacct agaaccgatt tatttgataa agatggaaac aattataaat
     7441 ctgatgtaat ccataaaggg aaaattgatg gaaaagatat agttttaaac actccctatc
     7501 atattccggt actttctgtg aatgtggatc ggattcaaat tgatacggct agagctgtta
     7561 aaaatggcta tttacctaca aaaatagaac taagtaaaaa agaagaatat gaaaaagaat
     7621 tagaaaaaat tgtattcaaa gttaaaatta cagatataga taacaataaa gttgtttatg
     7681 atgaaaactt taaatacaaa gaatttaata gtcatatgtc gcttgattta cctacagaat
     7741 atttaacaaa gaataaaaaa atcaattata gtgttgatat tcttttagtg gataatccag
     7801 ataaaaacaa gttcgaaact gaaactaaag atttaagaac gatcggttat acttcttctg
     7861 aaaaagtttt aacaaacaaa gatatgacaa ataaccaagt caactattca gcagtagtta
     7921 gaacagtgaa agaaagagag aataataatc cggtcaaaga gttcaatgag agtatcaatt
     7981 ataaatttac tgatagcact aaaacaaaaa caggatatgg tatatcagtt gatttagagc
     8041 caacatatag aaatgaaatt gggaaacaaa tagatatgaa agtctatagt tatccgatga
     8101 atgaattgat tgacagcaaa attgacgaaa tctatcaaag tgggggaaaa gaaacagaag
     8161 ttaagatgga tcgcttaaag aatgatggta aagttgagtc gggtattagt actcaaaatc
     8221 tttcattcca atttccacat atgaatatcg aaagaaaaac tggaaatata tatgctgaca
     8281 gtcaaagaaa tgatgaatct attaagtatg aattgatcga tggcggtaga aaattatatg
     8341 tgcctatttg gtgtgatttg ggaaattatt ctattgctat caaaagtagt aactttggtg
     8401 gtaactatat ctcgttggat atgaaccaga acatagatgt ttatgcttat atgtatgcaa
     8461 cgatagatag tgaaacaaaa aaagatgatg aattactgct tgaacctgtt tatccagagt
     8521 ctactctacc agatggttgg agcaaaaatg agattgattg gctgaataaa taagtttgaa
     8581 aagtttttat ctatctgata gaattaaata atgatatagg gatggtgata gaatgagggt
     8641 tcaaagctat ggtaacagtg tctttgatgt taaaagagaa tttcaagatt ctagaagaaa
     8701 aaccaaaaag ctgatagctt acttcattct atcagctatc gttttctata ttgtcaagtt
     8761 tgttttggaa ttaaactcaa tagtattcac agatatggtt cataaaatgt taaatggaga
     8821 acctattaat ttcaactttg attacatgct tgaaaaagca caaagcaatc aaattgtggc
     8881 ttttatcgtg agtatctatt attttattga aaaatctatt ttttctttaa aagatttttt
     8941 ctttgaaatg cttaccaaac taacaacgtt ttacaataaa atttcaaaaa attaaggagg
     9001 aagtttgaat gttagatcaa aatacagaca gaagttggtg gatgattggt gccgtgatcg
     9061 taggtgccat cttaatcggg acagctaaag tagcttttcc cgaagtgttt aatatcgtca
     9121 ttacattttt caaaaacatg atccctacaa acttaggata gttccactaa aaaattcacg
     9181 tagtttgatg cgtagaaaaa taataaagaa tgaaaaaggc tacataaagt gatttatgta
     9241 gccttttttt tggatggaag tgatgtaatg ctaagagata tgcccaaaat gattctttta
     9301 atgtttgttg ggttcttttt aattttagtg attgcgtact attcattaaa tggctataca
     9361 caagattcgt ctgtgaaagg tatgcaagaa acgttaagaa ctacagcaat agcaaacagg
     9421 gatgatagta ttcgagtaga aaaaggacga tttgctttaa ttaagtctga ttttgaaaaa
     9481 gattttaaag aaaaatttgg gaaaagtaaa aatctaaata atgaaaccgc tgattataaa
     9541 tttgattatc ttacagatgg gaaggacggt attaaagcaa ttaaagtgaa ggttgaaagt
     9601 ggatcaaaag aatatcaagc aacttgtatt ttaaatgttg caaatgatta gaggtgtgaa
     9661 atttgcgtgt tgtagaggaa aagaagattt ctccaaaaat cgaggaaact cctaaaagta
     9721 aaattaatat cgcaagtatt attaaaaaaa atggaattaa agaaaactct gaagcgatag
     9781 aaggaaacct tgatttttta actaagcttt tagaagaaga tagtggaaat tatgaaccac
     9841 caattgaaga tgcaaaatta aatatgattc gtgatgattt aaccagaaac tattcccaag
     9901 aagttttaca agctttaact gatgatgaaa ttaggcgaaa acttgtacaa gttgttttga
     9961 aagaacatgg tactttcttt aaaaaagata aagaggttgc agaatatgta gtacgtgaat
    10021 gtattggaac agggattatt gagcgaatta tgaccaacga tgaaattaca gatgttggtt
    10081 ggaatggctc tcaacttagc attgaaacta actctaacaa atggttgttt gacggtgata
    10141 aattaaatat cacgcaagag tatattgaac gtgttatttc taaatatgct gttgtaaatg
    10201 atcgggcttt taatagtggt aatcctatca tggatggaat gtttaaaaat gttcgtatat
    10261 cagctacaca catagataat tcaccagatg gctctacgtt ttctatgcgt gtggcaaggc
    10321 ctagattagc attgaataaa gaaaactttg aagattttgc tcctgattat attttggagc
    10381 tatttgagaa aatgatgaaa acaaaagcaa atatctgtat atcaggggag acaggtacag
    10441 gtaaaacgga acttcaaaaa ttactgctaa gttttattga taatcatgat cgtatgatta
    10501 tgattgagga cgttcaagaa actcacgcta aagaattatt tccggataaa gatattttct
    10561 cttggatcac tactcccaaa aaagcaattt cagatttagt aaaagcttct cttagaaata
    10621 atccacgatg gatcattgtt tcagaaacac gtggttctga agcttatgaa atgcttcaag
    10681 cggttttgtc aggtcactat gttgtaacga cacttcatgc ggttaatgca cgtgcgatac
    10741 ctagacgttt tgtaaatatg tgtgcaagtg gttttaaaat aaacgaaaat gcggttgagg
    10801 aagacataaa tcgttatttc gattttggtg tccatatcaa aaaaacagtg gttgatggta
    10861 aagtgatccg ttatttgaat gaaattgttg aattttctgc tagtgaaccg actacagttt
    10921 ttaagcaaaa atttatcaat ggaaagtttg tcattgaaaa tggtagtctt tccgaagatt
    10981 ttaaagaaaa aatggctgaa aaatttatta cgtttgaatt tcctgaagat caaactaata
    11041 cagaagaatc acttcctgaa gaagaagagg tgatttaagt tgaaagtaca ggctatgaca
    11101 aagcgaaaag gtctcatgga tgtttcggaa ctaaacgcct atcaagtagc gtatggtaaa
    11161 ccgttagatt atagagatta tttgaactat gttggattac cttgtttgtt tatgggtggc
    11221 ttttcttttg ctttatgtta ctactggtgg attgctttga tttgtggagt aattggaggc
    11281 gtatatggct ttaaagttat aatgcctaaa agtattaaac gctcttatga attaaagagt
    11341 ttacaggaaa gaaataaatt tattaacaac atgactcaaa ttttgaccga tgatagtaaa
    11401 acagttacta gagcgttatc tatagcaaaa cagcgaacaa aaggtgaatt aagagaagat
    11461 attcaaatct tagaatcacg tttacaaggt gctgataaat ttcagattgc tgaagctttt
    11521 cgtgaaatca acaataaata ttatagtgat gttgttttta cacaatactt tgagcaattg
    11581 gaaacagcta tttacgaagg tagaaataat tcagatacta tgaagcaaat aaagagctat
    11641 cataatgatg tgaaacgtaa aacagaaggt tttatgaaaa tcaaagacgg ctatcttcaa
    11701 gatatgaagc aaatgatgtt tattgtagct gtttttattg gagctataac ttttgctttt
    11761 ggtttttcta ctttctatga aggttttgct cgatcaccta tcggatgggt atttggatct
    11821 atttactttt tgattatggt tagcttcatg aagtcatttt tcaaacatta ttttgatgat
    11881 gaaataatga gtttgggggt aagtaattaa tgaaagttaa ggctttaaaa aagaaaagaa
    11941 aatcaagtat tagccccttg aaaattacga aaacagaaca taaaagcttt tggattaaat
    12001 tactaggtga tgtttattat caaaaattac gtgaaatgaa caattccaat attgcgatta
    12061 ttaagtggca aaaacaacgg ttaaaaaagt cattgatttt tatcctagtc ggattcttaa
    12121 tgtatctatt tttagggaaa gaatggtatt acttagccgg tggaattgct acttcagtag
    12181 gtttttatgt ttggcaagca aaatcaatca atggtatcta taatcaattt cggtttgaac
    12241 gacacttgca atttagtaaa tttacacggc tattgatccc ctatctgaaa caaaaaaaag
    12301 atggcggaaa tttatatggt gtttttgcaa aaatcgtaaa aagactagat tatgataccg
    12361 ataaaaagct attaatgaag cttatgcaag aaatgacaga tcagcctaac gatattagac
    12421 cttttacgga atatgcagaa cagacttctg gaacagacat gtccgtatta tttatgtcta
    12481 cagtttatga tattcgacaa ggttcggcag atttggatgt tattgaagag ttggatcgtt
    12541 tagcttctga agagttaatg gccggaatcg attcaatcat tgactataag tcgaggcgtt
    12601 ttgtcttctt tcctacaaaa attgcaatga cttcatttat tttagttgtc ggttttgcag
    12661 taggcgttct tttcatgaac ctaaaagata ttaactttgg gcaataaagg aaaggagaaa
    12721 gtcaatgcaa gaacaatccg ttatggaaat cattaaatgg tttataggtt taatgatgat
    12781 tatggcaatg gtttctatag gacttttttg tgttcaatta caagatatta atactttcaa
    12841 gcaacaagta aattatcaaa ttgaacgaaa aggaggtctg acgacagaag ctgttgaagc
    12901 aatcgacaaa tattcggcta ctaattatga tagacgtttt acaattgaaa gcgataagtt
    12961 aaatcagaaa gtatcttatg gagaagaaat tgattatacg atcaaagcaa ctattccaat
    13021 tcaaatttta ccgcttcctg atgtaacttt atcgtttaac ggaaatagtg tttctcaaat
    13081 aagatgagag gaaggataaa atgcaagaga ataatgtttc cagaataatt tatttgattg
    13141 gcggattgat aattttatcc ttagctattg ccggtcttgt agagcattgg ccggaaataa
    13201 agttattaat aagaaacttt tacgacaata aaatgattaa aatgtaggag gtatagattt
    13261 ggaagcaatt caagaaaatc gtaaggataa tcaagaaaca agtgaaaaaa acaaaaaaaa
    13321 aagaaaaaag aaagtagata aatttacgga aatgaccgat aaaatcattg aaaagaaagg
    13381 acttaaacct aaagactttg accgtcaagc aatcaaagaa gcacgacaaa aactgtatga
    13441 tgattttgtt aaagaaaaca tatcacagct tttagactaa catttagggg gaaataaaat
    13501 gaaaaattta gttatcagca agtttgcaat gttaccaaga atggattttt tcgataatgt
    13561 aagtaagttt atgtcaaata tgacttcaaa actacaagga atttcactat ctgtaattat
    13621 cttttgtatt gtggttactg gtttgatgtt tcttttcgga gaagggccaa gtcgtactgc
    13681 taaaaaatgg ttaacttata tcgtgattgg ggcaataatt atcttcggag cttcaactat
    13741 cggacaaacg atcaagtccg ttggtggttt ctaaggggaa gcttcttaga actaaaaaat
    13801 tcgcaagtat tgcatgaaag agcattgagt gattaatgct cttttttatt ttgaaaaact
    13861 ataaactaag gagatttaaa ctatgaacca acgattcgaa tataattttg aagatgtaga
    13921 acctaagtta ctgaaaaaag gaaaaggatt aaaaaagaaa aataaatata ctctcaaaca
    13981 agcagaagca ttagtaggat ggattcaaca caattggtca gacaagacaa caattacctt
    14041 gatctgttca gacacagaag aaggaacaaa gtatgctttt gatggaattg aaattactcc
    14101 attttcgatt ggcggacaag aaaaattatt gtttcctgat attgaaggac aaattttaca
    14161 agcgccggac agcatgattt ccgatcctaa taaactgatt ttggatatta acgatcaata
    14221 caaacacgaa ccagacgtag cagaaccaac aaaagaatca aagtcaaaat taaatttgaa
    14281 gctcccttca gtagtattac catttgctaa gaagaaaaat aaacttatga acgaattgat
    14341 tgaaccagaa gaaacggata tttatgaaga agaattagaa gaacaagaag agatcgaaga
    14401 attagttgtg tcttctgaag aaacagaaga agtagaagaa attgaaactg tttctgaaaa
    14461 tgaagaagaa acagaagaaa tctttgactt tgaagatgaa gaagaagtgg aaaatgtaca
    14521 gaaaggcgat ctatctacaa gtagtcaaaa tgggttggaa gctgtaaatg atccacaagt
    14581 tccaccatat atatcttcac aaactcccaa ttactctcca cagtatcaag cgcctaatgt
    14641 cttacaacca ttaagaaaac acgaaacagt gcagttagtt acgcttaaag agtactgtga
    14701 tttgggagaa gaaattgaaa cttcaatttc tcaaatggtc gaaaagttga aaccagaatc
    14761 tttattcaaa ttcataggga ttccttctac aggtcttaca aatactcgta ttgatgaata
    14821 tagaagtaac catgctatgt tgcgattagc tgaagtaaaa tttgaaaatt taagagagca
    14881 ttattcacgg caaattattt cttttaaagc agaagcccta gatcgtttaa gaactgcggt
    14941 agatattgct tggaagaagc cttacgattt agatgtgaag aaaaacaagg ccgaagagtt
    15001 atcaaaaatg gaagcagaag cagaagctaa attaaatgag ttcgtgaaac gtcaggaaaa
    15061 caatgtaaat gataaaatca aaaagtttga gatcgaacaa gaaatagatt taaaaaactt
    15121 tattgcaaga caagaggcag ataaagcggt atttattgct acagagaaag atcggactca
    15181 aaatttaatt gattctaaag cggaatcaat caatgcagat ttacttgaaa caaaagataa
    15241 atttattgat gaagaaatgt acaaaatgaa aattgaaatg aacaaaaatt tatttgatgg
    15301 caagcaaaaa attaaatcag aattggctac tgaactcgct aaggcaaatg ataatgtttg
    15361 ggagaatgcg ctgaaattta ttcaagaaat tcaagatgaa attgaagcta aaacaccaat
    15421 ttgggctgct gaaatcaagg agtttaatag tttagaagaa caagaacatg aacgtatgaa
    15481 gcaaactaaa gaacttcaat taaaagagga aagtattgaa atcaagcgtt tggaagctga
    15541 aatgaatttg aagcgtatgg aagaattaga tcatgaaaat aaaatgctta aaattaaact
    15601 tgaaactgcc ctttcaaaaa atgagttatt agctatggaa caagatacaa agcaagtggt
    15661 gaatggatca aacagagttg gtgcacttaa tttatttagt aaacgttaaa ggagaacgga
    15721 tatgagtgtt tttaataata aagttatgtt agggaactgt tatgtgaacc acattggttt
    15781 ttacgatgga ggaacataca gagttttatt cttacgttta tctgttttta gttactcaaa
    15841 agatggaaag aggcattatg aaaatattaa tgcacaaatt tcttgtcctt tagatagaaa
    15901 tggggaaacc acgactacaa gtagtcaccc taaagccttg atgaataaat ttatctttga
    15961 aaagttggat cgcaatttgg ttgaggggat gaagataagt attcatggaa atattcgtgg
    16021 aacagaacaa gtgcttctta acagtggttg gaaaaacttt ggtcaattat cgcctttaga
    16081 aaaagaagaa tatcaaaaaa ttcctaaaaa tgaattagta acaagaaatc aaacatatat
    16141 ctatatagaa gaggtaagta tgccagaaaa agaagaattt agaaagtctc aatctaaggt
    16201 aacaaaagaa gatgtggaag ttgcagaacc gaatcaagca gatgaaattt tatccgatga
    16261 tactgatcca gtgaatgatg aaggagaggt tattgaagtt tttgaagctg aaattatcga
    16321 agaggaagaa gaaattaaaa gttttgataa aagaatgtat cgagagaaac caaaaggtta
    16381 tgtgaatttt gaagatatgt attatgacga atattctaat gaaccaccga tcaattagtg
    16441 ggggtcaaat atgaagctag tacaaaacaa tgaagtgaaa gaaataaaaa acggctttgt
    16501 taaaaactcg aacaaaattt tgggaaagct gaaaaaagta ggtagtttca aaaaaaataa
    16561 atatcatact ggaaaaattg agggagaaga tttaccaaaa agtttacaag ataaattgaa
    16621 tgttccgctt gtatttatag gtgttttttt aggttcaata gtttttctag caataacgta
    16681 cttattgaac tttttaaaag ctttgttagt tgcaatcgga cagtcagaaa gtgggctact
    16741 tggtgtagag ttcgatccta agtttagttt ggcatatcta aaaccacttg gggatacgaa
    16801 acaatacttt atgtgtgctg tggttgcggt aataggagtc gcttatttaa tatatgggaa
    16861 attaaatcat gaaagtgaga aaaatcttgt ttacggtcaa aaaggagatt caagactttc
    16921 aactatcaaa gaaattaaga gagattacca tgagatacca gaaaaaacag aagtgtatga
    16981 tggtattggt ggtgtgccaa ttagtcacta tcaagataaa tattatattg atagagacac
    17041 agtaaatacc gttgttttgg gagcttcacg atccggtaaa ggggaaacat ttgttgttcc
    17101 tttattagat aatcttagcc gagcaaaaat acaaagtaat atggtcgtga atgatccgaa
    17161 aggagaattg tttagcgctt caaaggaaac acttgaaaaa cgaggttatc gtgtattagt
    17221 gctaaatatt gatgatccac ttgaatcaat gagttttaat cctttacaac tagtaattga
    17281 tagttgggct aacaaggact atcacgaagc tagtaagcga gcgaatactt taacttcaat
    17341 gctctttgct tcaggaatgg gtacagataa tgagtttttc tataaatcag ctaaatcagc
    17401 ggtcaatgcg attattttaa cgattgtaga acactgtttt aacaatgatt gcattgaaaa
    17461 aattacaatg tacaacgtgg ctcaaatgct gaacgaatta ggaagcttat tttacactga
    17521 tccaaagact agaaaagaaa aaaatgcttt agatgaatat ttcaatactt taccacaagg
    17581 gaatcaggcc aagattcagt atggttctac ttcatttgcc ggtgataaag ccaaaggttc
    17641 gattttatct actgcttcac aaggtattga aatgtttaca agtgatttgt tcgggaagtt
    17701 aacaagtaaa atgtctattg atttgaaaga gataggcttt cctaagtcca tacaatttaa
    17761 agtcaatacg aatttagttg gaaagaggat tagtatttca tttttgagaa aagaaaatga
    17821 tgtaattaga ttaattaaga agtatcgagt gaaagtaaaa gctttagggc tatgtgtttt
    17881 aaattttaat gaatatcttc aggatggaga tatgttggat atacgatatg aagaagagaa
    17941 atcgagagca tggtatcgta ttgaatttcc caaaaaacaa gcaaatagtc atgatacttc
    18001 tttagtcact aaaaaattca agtcaggtaa aaattattct gacttagaaa tcagcacctt
    18061 acgattaaaa tacacggatc agccaactgc tgtgttcatg gttattccag attatgatcc
    18121 gtcaaatcac gtacttgcaa gtatttttat cagtcaactt tatacagaat tagcaagtaa
    18181 ttgtaagcaa acaccggata aaaaatgttt taggcgagtt catttctt
//
LOCUS       DACDCJ010000060        17785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDCJ010000060 DACDCJ010000000
VERSION     DACDCJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.060"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1300
                     /locus_tag="IV038_001990"
     CDS             <1..1300
                     /locus_tag="IV038_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP8342191.1"
                     /translation="EKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKITE
                     KAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQLI
                     DWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLVN
                     LNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEIK
                     NVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQYG
                     DNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAPI
                     LGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFYR
                     NARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1369..1968)
                     /locus_tag="IV038_001991"
     CDS             complement(1369..1968)
                     /locus_tag="IV038_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8342192.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            2222..3103
                     /locus_tag="IV038_001992"
     CDS             2222..3103
                     /locus_tag="IV038_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8342193.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            3116..4726
                     /locus_tag="IV038_001993"
     CDS             3116..4726
                     /locus_tag="IV038_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP8342194.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(4803..5462)
                     /locus_tag="IV038_001994"
     CDS             complement(4803..5462)
                     /locus_tag="IV038_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP8342195.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(5745..6410)
                     /locus_tag="IV038_001995"
     CDS             complement(5745..6410)
                     /locus_tag="IV038_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342196.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(6557..7162)
                     /gene="dhaL"
                     /locus_tag="IV038_001996"
     CDS             complement(6557..7162)
                     /gene="dhaL"
                     /locus_tag="IV038_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP8342197.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(7190..8173)
                     /gene="dhaK"
                     /locus_tag="IV038_001997"
     CDS             complement(7190..8173)
                     /gene="dhaK"
                     /locus_tag="IV038_001997"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP8342198.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(8175..8570)
                     /gene="dhaM"
                     /locus_tag="IV038_001998"
     CDS             complement(8175..8570)
                     /gene="dhaM"
                     /locus_tag="IV038_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP8342199.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8724..9044
                     /locus_tag="IV038_001999"
     CDS             8724..9044
                     /locus_tag="IV038_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP8342200.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(9156..10025)
                     /locus_tag="IV038_002000"
     CDS             complement(9156..10025)
                     /locus_tag="IV038_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8342201.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(10121..11425)
                     /locus_tag="IV038_002001"
     CDS             complement(10121..11425)
                     /locus_tag="IV038_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP8342202.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(11422..11931)
                     /locus_tag="IV038_002002"
     CDS             complement(11422..11931)
                     /locus_tag="IV038_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342203.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(12106..14241)
                     /locus_tag="IV038_002003"
     CDS             complement(12106..14241)
                     /locus_tag="IV038_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8342204.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(14324..15979)
                     /locus_tag="IV038_002004"
     CDS             complement(14324..15979)
                     /locus_tag="IV038_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8342205.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(15981..16295)
                     /locus_tag="IV038_002005"
     CDS             complement(15981..16295)
                     /locus_tag="IV038_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP8342206.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            16465..16725
                     /locus_tag="IV038_002006"
     CDS             16465..16725
                     /locus_tag="IV038_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP8342207.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            16739..17053
                     /locus_tag="IV038_002007"
     CDS             16739..17053
                     /locus_tag="IV038_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP8342208.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         5017 a         3622 c         3229 g         5917 t
ORIGIN      
        1 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
       61 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
      121 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
      181 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
      241 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
      301 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
      361 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
      421 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
      481 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
      541 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
      601 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
      661 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
      721 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
      781 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
      841 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
      901 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
      961 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
     1021 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
     1081 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
     1141 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
     1201 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
     1261 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
     1321 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
     1381 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
     1441 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
     1501 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
     1561 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
     1621 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
     1681 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
     1741 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
     1801 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
     1861 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
     1921 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
     1981 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
     2041 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
     2101 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
     2161 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
     2221 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
     2281 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
     2341 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
     2401 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
     2461 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
     2521 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
     2581 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
     2641 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
     2701 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
     2761 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
     2821 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
     2881 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
     2941 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
     3001 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
     3061 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
     3121 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
     3181 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
     3241 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
     3301 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
     3361 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
     3421 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
     3481 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
     3541 gcttttagca atatcgatca gttgtacgat gatttttttc caagcggatt cgatcactgc
     3601 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
     3661 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
     3721 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
     3781 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
     3841 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
     3901 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
     3961 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
     4021 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
     4081 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
     4141 tgaaaaaaac tttacaagca cgatcatggt cgtcttgatt tgtctagctg ctattttacc
     4201 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
     4261 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
     4321 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
     4381 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
     4441 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
     4501 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
     4561 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
     4621 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
     4681 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
     4741 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
     4801 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
     4861 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
     4921 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
     4981 ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa
     5041 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
     5101 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
     5161 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
     5221 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
     5281 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
     5341 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
     5401 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
     5461 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
     5521 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
     5581 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
     5641 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
     5701 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
     5761 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
     5821 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
     5881 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
     5941 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
     6001 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
     6061 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
     6121 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
     6181 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
     6241 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
     6301 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
     6361 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
     6421 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
     6481 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
     6541 cgcagtttta ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg
     6601 cactagaagc agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc
     6661 gtcctttttt agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg
     6721 ttaaattccc tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat
     6781 cgtcagcttc tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc
     6841 ctaatttttc ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga
     6901 atgcagatcc gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga
     6961 atactttgaa tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag
     7021 ctgaaacccc tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat
     7081 cactgagata ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga
     7141 tttcttgaac ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa
     7201 tcgtctgtac attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg
     7261 ttagagaaag accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta
     7321 cattgacgcc tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca
     7381 tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt
     7441 aatcatctaa aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt
     7501 ctcgacgata gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta
     7561 gttcgaatcc tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg
     7621 tcttagttgc ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt
     7681 ttcttgctgc atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc
     7741 cagcagtata cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt
     7801 caatatcgtc catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat
     7861 aattcttaac gatcagcaaa actccttttc cactatcggc ttccttaata gccgtctgaa
     7921 tctgatctgg agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt
     7981 cccctacaaa cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa
     8041 cttgttccgc tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt
     8101 ctggatagct ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca
     8161 tgattttttt caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt
     8221 tgctgagtat catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa
     8281 ggcgcatcca ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt
     8341 tccgagttca ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt
     8401 gattcattga ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg
     8461 ccgagagaaa aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata
     8521 ccatctgtta ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc
     8581 ctttctttta catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat
     8641 ataataaatt tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt
     8701 aattaaaaga gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag
     8761 ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta
     8821 aagacttgga ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg
     8881 aactaatggg tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag
     8941 gagctgatgc gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca
     9001 tgctgatggt cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt
     9061 ttaataacta aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta
     9121 acttcgaggt actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg
     9181 taagctgctg cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt
     9241 tcatttgttt ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact
     9301 gtttctgtcg tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt
     9361 attcgttgga aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt
     9421 tgtgcttcgc tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt
     9481 tgacttagct ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt
     9541 gcttgttgga tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg
     9601 gcaagctttt tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa
     9661 tcttgatcga ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct
     9721 tcgagttctt ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta
     9781 gaactattta ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt
     9841 acattggaag tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt
     9901 gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag
     9961 aaaatactgg aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt
    10021 cccatcctta ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca
    10081 taaacacaac aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg
    10141 ggcttttcca tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg
    10201 tcacgatcgt tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa
    10261 ggtatttcaa ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc
    10321 ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt
    10381 cgtagtttcc ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac
    10441 aaaagaatat tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa
    10501 tatgtttaaa aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac
    10561 cttttgccca acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga
    10621 aaaagataat ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa
    10681 tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt
    10741 ttcgatacat cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg
    10801 ttcgtttaat gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt
    10861 tggtacgatt ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc
    10921 gaatgaaata attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat
    10981 tgcttaaaat caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc
    11041 ctttattttt ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta
    11101 atatttctgg tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat
    11161 aattcaattt cgtcatcaaa tactcgattg tatattcgat cacaatttct tcattatggg
    11221 ctggcaccat gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt
    11281 gcttatgttt gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta
    11341 aaatcggata agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat
    11401 tacctgtatg ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc
    11461 aatgaatgtc gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct
    11521 ctcttctttt gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt
    11581 caatttttca tcttcatatt caaaagattt atttactttt ttgtatagat ttcgattgtt
    11641 ccacctagtc aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc
    11701 gattaagaaa tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc
    11761 tctttctggt ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca
    11821 tcctaaaaga gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata
    11881 cttatctttc accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc
    11941 tcacgttttt ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag
    12001 tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata
    12061 ttttgttctt tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc
    12121 caatacgaca atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct
    12181 ttgctcatct ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt
    12241 tgctagactc aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga
    12301 ttcttgtagc gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt
    12361 tagagaaacc aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg
    12421 gtattcgggt tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac
    12481 agcataggtt ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc
    12541 ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt
    12601 ggaagaatta aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa
    12661 taattcgatc aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag
    12721 atagcgaaat gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat
    12781 gactgcttca tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc
    12841 ataaattctt tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt
    12901 tatttttcga tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga
    12961 atcatcagaa atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac
    13021 ctgattctgc acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat
    13081 cagtgccttt ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc
    13141 atatattttt tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg
    13201 gataaaattc ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt
    13261 tttgctacgc aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata
    13321 agcctcttca ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag
    13381 attcagtgga tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg
    13441 atgattaggt gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag
    13501 cagatacgca atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac
    13561 caagatattt tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc
    13621 agtgatagcc ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc
    13681 ttgaatagct acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg
    13741 tcgtctttct ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc
    13801 aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc
    13861 atctgcatca tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc
    13921 cagaaagtct acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc
    13981 tagctgattt tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat
    14041 ttcaatcatc gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc
    14101 gccagccatt tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg
    14161 cagttcgttt tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa
    14221 ctgtttttgt gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta
    14281 gtttacctct tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata
    14341 tcagttcttc aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt
    14401 ggtagccaga ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc
    14461 tgacgatata ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag
    14521 ggatatgttt ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta
    14581 ataaaatttt tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa
    14641 gcccttctgc aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc
    14701 caaatggcgg tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt
    14761 tgattgctgt tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg
    14821 caccataaat ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt
    14881 ctggataata gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt
    14941 tttctttggc tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta
    15001 taattgctgt tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa
    15061 cttgtctacc ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga
    15121 tttgctgttt tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat
    15181 aacgttcgtc tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta
    15241 gctggttatc aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc
    15301 catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa
    15361 tcacttctac tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca
    15421 gtgttggaag aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta
    15481 ggttttctgc catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct
    15541 gtttttctaa tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat
    15601 tgtcggcttc tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa
    15661 ccaatgcatc gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt
    15721 tttcgggaaa gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg
    15781 ataatgattc tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat
    15841 aataaggcaa gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt
    15901 ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc
    15961 cgacaataac gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg
    16021 ccaccacgta cattcactac atcataacca gatttcttta attctttagc tgcttgttta
    16081 ctcctcatcc ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg
    16141 tttatacttt gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca
    16201 ggtgttctaa catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact
    16261 gttatcgatt ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta
    16321 taaatacctc atggggtata ttaaacagga gctttattaa tatgtcaacc aaaatgactc
    16381 ttcatttttt tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat
    16441 tataaaaata gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg
    16501 aaacgtacag agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt
    16561 atggatgtca tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg
    16621 atcgttgcag ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa
    16681 gcggctaaac tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat
    16741 gaaagtcgtt ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa
    16801 aaacctgata aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat
    16861 taccggttat ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca
    16921 cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt
    16981 aatcgttgta ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta
    17041 tatcaagcct tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct
    17101 tatttttcga agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc
    17161 tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg
    17221 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    17281 ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt
    17341 ttgagctaat accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag
    17401 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata
    17461 aatttggctt aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa
    17521 tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc
    17581 gttttttgag ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag
    17641 cctaccattt aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg
    17701 ttcggttggc ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg
    17761 cgacataggt cacacccctt tttta
//
LOCUS       DACDCJ010000061        17397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDCJ010000061 DACDCJ010000000
VERSION     DACDCJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.061"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            223..591
                     /locus_tag="IV038_002008"
     CDS             223..591
                     /locus_tag="IV038_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP8342209.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            771..1712
                     /locus_tag="IV038_002009"
     CDS             771..1712
                     /locus_tag="IV038_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP8342210.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1781..2512
                     /locus_tag="IV038_002010"
     CDS             1781..2512
                     /locus_tag="IV038_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP8342211.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2656..4836
                     /locus_tag="IV038_002011"
     CDS             2656..4836
                     /locus_tag="IV038_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP8342212.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4856..5338
                     /locus_tag="IV038_002012"
     CDS             4856..5338
                     /locus_tag="IV038_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP8342213.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5354..6115
                     /locus_tag="IV038_002013"
     CDS             5354..6115
                     /locus_tag="IV038_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP8342214.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6246..7580
                     /locus_tag="IV038_002014"
     CDS             6246..7580
                     /locus_tag="IV038_002014"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP8342215.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7887..8372
                     /locus_tag="IV038_002015"
     CDS             7887..8372
                     /locus_tag="IV038_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP8342216.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8365..9048
                     /locus_tag="IV038_002016"
     CDS             8365..9048
                     /locus_tag="IV038_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP8342217.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9174..11819
                     /gene="polA"
                     /locus_tag="IV038_002017"
     CDS             9174..11819
                     /gene="polA"
                     /locus_tag="IV038_002017"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP8342218.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11864..12700
                     /gene="mutM"
                     /locus_tag="IV038_002018"
     CDS             11864..12700
                     /gene="mutM"
                     /locus_tag="IV038_002018"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP8342219.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12664..13293
                     /locus_tag="IV038_002019"
     CDS             12664..13293
                     /locus_tag="IV038_002019"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP8342220.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13345..13677
                     /locus_tag="IV038_002020"
     CDS             13345..13677
                     /locus_tag="IV038_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP8342221.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13989..14480
                     /gene="nrdR"
                     /locus_tag="IV038_002021"
     CDS             13989..14480
                     /gene="nrdR"
                     /locus_tag="IV038_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP8342222.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14503..15876
                     /locus_tag="IV038_002022"
     CDS             14503..15876
                     /locus_tag="IV038_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP8342223.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15876..16802
                     /gene="dnaI"
                     /locus_tag="IV038_002023"
     CDS             15876..16802
                     /gene="dnaI"
                     /locus_tag="IV038_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP8342224.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16931..17371
                     /locus_tag="IV038_002024"
     CDS             16931..17371
                     /locus_tag="IV038_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP8342225.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5936 a         3041 c         3818 g         4602 t
ORIGIN      
        1 tttttagtca cgacctcttt ttttatttat tccatataaa tacacattat ttattccgaa
       61 aagtgaaagc gcaaataaca aaaacaacca acttatttat tattaaagaa aagcgtattt
      121 aagtaggacg attctgttga gagctttcgt agattttccc attttcctat ggtaaactga
      181 caaagttgag aatgttagaa cagatcgttt aggaggaaac agatgtttac gaatgaaata
      241 aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa cgttttggag agcagttggc
      301 tttgcggaaa aagatcgtca agaaatggat gggacgctta taattgaagt aggagttgag
      361 gacagtcaaa cctcatttct gttatatgat cgagcatttg ttgagtccca ttcacctggc
      421 acagctacta gtgccccatc tttgatgttt tacagtaaag atatctttgg actatataaa
      481 aaaatgcaga cgcaatcagt cacattaggt gatatggtac agcttggaga agaatatgtc
      541 tttaactttg ctgatcccga tggcaactac tttgcggtta caggtaaata atagaaataa
      601 agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt tttaattcaa ataataatat
      661 tatgtaaaca aaaaactttc catgcgtaat tatacacaag caaataaatc gtcaaatgcg
      721 aacgccttct ttatactgtt taggaacgaa aagaaggaag gaagaaatac atggattatc
      781 aagtactgct ttattacaat tatacaaaaa tcgaagaacc agaggaattt gccaaaaatc
      841 atttagcctt ttgcaaatct ttggatctta aaggtcgtat tctagtagct gaagaaggaa
      901 taaatggtac tgtgtctgga accgttgaaa acacgactgc ctatatgaaa gcaatgaaag
      961 ctgatcctcg atttgctgat acgatgttca agatcgatcc aagcgaagga catgccttta
     1021 agaagatgtt tgtaagatac cgtccagagc ttgtaagttt aaagctagaa gaggacattg
     1081 atccaaacca agtgacaggt gcctatcttt caccgcaaga atttaaagaa gctattttag
     1141 atgaacagac agtggtgatt gatgcacgga atgattatga atatgacttg ggacattttc
     1201 gaggggctgt acgcccagat atacgcagtt ttcgagagct tccagactgg atccgcgagc
     1261 acaaagaaga atttatggaa aaacgggtag tgacctactg tactggtggc attcgttgcg
     1321 agaagttttc tggttggctt gtccgtgaag gattcaaaga tgtcggacag ctgcatggcg
     1381 gtattgctac ttatggtaag gatcctgaag tccaaggaga attatgggac ggcaagatgt
     1441 atgtgtttga tgaacggatc gctgtcgata tcaaccaagt agataaacaa atcgtcggtc
     1501 gcgactggtt tgacggaaca ccatgtgaac gatatatcaa ttgtagcaat cctgagtgca
     1561 atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca tttaggagca tgtagcatag
     1621 agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca tggaatctct ccagaagaag
     1681 cagaactgcg gtttacagct ttaattaaat agaaaacaat agttttgatt gtcgaccatt
     1741 tccaatatgc gctatactag cgttgaggat ggtgatccac atgaaatcat tgatttatgc
     1801 tattggggat gtccatggca aacatgagct ttttgaaaaa cttattcact attatgatcc
     1861 aaagatccat cagcttgttt tattagggga tctcaatgat cgaggtcctc gtacaaaaga
     1921 atgtttttta ttaggaaagc aattagtcga agaaacagga gccatttacc tccggggaaa
     1981 ccatgaagaa tattttttgc aatttctgca atcaccggag gactggtatg cgccttatat
     2041 gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac ccaggggcat cggaggaata
     2101 ctccccaact gaaatctcga tgatgatcca atcgagatat tcagaactga ttaaattttt
     2161 agtccagcgc cccttgtatt atgaatggga aaattatctt tttgtccatg ccggtgtgga
     2221 tttgacaaag aaagactggc gtaagacaag tctaagagac tttatttgga taagggaacc
     2281 atttcatgaa gggaaaaaca atacaggtaa gacaattgta tttggacata cgattacacc
     2341 aatgctccat ggagatatgc agacaacaga cctctggatt caggatcata aaatcggaat
     2401 ggacggtggg gctgtattcg gagggtcatt gcatggggtt gtttttgaca aggaaggtat
     2461 tcttcaagat attgaatatc agaacaacga aggaccttgg catggaatct gactaatgga
     2521 aagaatgaag gagctgtgac tttgttgcgg ctctttttat ttttatagag aatattgttt
     2581 ctttcagtag tttaacccag gataaaaagt atgatagaat aaagaagcaa tttttagcag
     2641 aaaagaggca ttttaatgac ggaaaatttg aaccagacga tcattcagct tgtacaaaaa
     2701 gaactttcag actatcgtcc taagcagttg actactgtac ttaatttgtt aaatgaagga
     2761 aacactgttc cttttatcgc gcgttaccga aaagaaatga ctggaagtct tgatgaagtc
     2821 cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa acttagaaaa acgtaaaact
     2881 gaagtgattc ggctaatcga tgaacaaggg aaactgactc ccgagttaga agcagaaatc
     2941 acccaagccg tgaaaatgca acaagtggaa gatttgtatc gtccatacaa acaaaaacga
     3001 cggacaaaag caacgattgc taaagaaaaa ggacttgaac cactcgcgaa gtggctgatg
     3061 cagcttacag atggagaggt gcaatctgaa gcagaaaaat atattgacaa agaaaaagaa
     3121 gtctcttctg ccgaagaagc gttgcacggt gctcatgaga ttattgctga acaagtcagc
     3181 gataatgcaa aatttcggac ttggattcgg tcttatacgt ataacaaagg tatgtatgtc
     3241 agcaatgtca aggatgagca agcagatgaa aaaggcgtat atgaaatgta ttatgacttt
     3301 gcagaacccg tccataaaat ggtttctcat cgaatattag ctactaatcg tggagaaaaa
     3361 gaagatattc tgaaagtatt ctttcaagta gatgaagcag caattttagc ttacttggat
     3421 cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat tcgtcagaga agcctatcag
     3481 gatagttata aacgctttat tcagccagca atcgagcgag aactgcgcaa tgaattgact
     3541 gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa acctccgtaa tcttttgttg
     3601 caaccgccac taaaaggtaa agttgtcctg ggatttgatc cggcttatcg tactggatgt
     3661 aaactagcag tcgtagatgc gacagggaaa gttttagcaa tcgaagttat ttatcctcat
     3721 aaacctgcag cgcaagcaaa aagagaagct gctggaccag cttttatcca attaattaat
     3781 aaatatcaag tagatatggt agctatcgga aatggtacag ccagtcggga atctgaactt
     3841 tttgtggctg agcagttgaa atccgccgac cataaaacct attacgctat cgtcaacgaa
     3901 gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa aagaatttcc gcatctacaa
     3961 gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc aagatccttt agctgaatta
     4021 gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc aacatgacgt ttctcaaaaa
     4081 cgtttggctg aacaattgga ttttgttgtg gaaacagcgg ttaaccaagt aggtgtggat
     4141 gtgaatacag caagcccgca gctcttgcag catatatcag gattgaataa aacaactgcc
     4201 caaaacattg tcatttatcg tgaagaaaac ggagaattca ccgcacgaac tcaattgaaa
     4261 aaagtgccac gcttaggacc aaaagcatat gaacaagcaa tcggattctt gcgagtccct
     4321 ggtgggaaaa acattttgga caacacaggt atccatcccg aaagttattc tattgcaaaa
     4381 gaaatattaa cttcagtcca actttcagaa aaagagcttg gtacagaaga agcagtagaa
     4441 aaattgacac gattatccgt agagaaactg gctgaaagcc tatcagtcgg agaagaaacg
     4501 ttagccgata ttttagctgg gctgacacaa cctggacgag atatgcgtga tgagatgcca
     4561 gcgcccttat tacgtacaga tgtattgagt atggaagact tgaaaccagg gatggaattg
     4621 actggaacgg tccgtaatgt cattgacttc ggcgcatttg ttgatatcgg tgtgaagcaa
     4681 gacggacttg tccatatatc gaaactcagc aaaaaattcg tcaagcatcc tacagatgtc
     4741 gtgtcagtag gagatatcgt aacagtttgg atagaacaag tcgatacgaa aaaaggtaga
     4801 atcagtttga cgatgctttc cccttatgaa gagtaacaat gaggagtaac aaaaattgac
     4861 acaagaagaa ctgcagcagt tagtagaaga gatatcatta aaaagcttcc agcttccttt
     4921 caagcaccaa gcaatgttca atccacgctt acgtacaact ggcgggcgct atcatttaaa
     4981 cgatcatcat ttagatttta atgcgaagct atttgaagag tcagacgaag agacgattgc
     5041 aggaatcatc aaacatgaac tttgccatta tcatcttcat ctagctggaa gagggtatca
     5101 gcataaagat atggatttca agtatctttt gaaaaaaaca ggagggttgc gctatgcacc
     5161 agccttgacg aagaaacaga taaagatgga gtattatcga tgccaagatt gttctgccac
     5221 gatctgccgc aagagaagaa tcgatgtacg caaatatcgt tgtggaagat gtcatggaga
     5281 aatcaaatgg attgagacaa aaagagaaga ctttaatgaa gcacaaaaaa tcagatgaaa
     5341 ggatagaagg cctttggaaa aaagtgagca gaggaatcta aaagactgga atcagctatt
     5401 aaatttttta gccgcacctg aagagggaaa ttttgaggaa ctgacggcac ctacacttat
     5461 tttagctgga aactgtcttc ctatcttagc tgataaagca gcacaaatgt atttaaatgg
     5521 ccaagtagat caattgtttt tggtcggagg ggtggggcat gcgactagaa tcctttatga
     5581 gaattttgaa aagcaaggat ttcattttga agatggcatg agtgaaagtg aaatctgtcg
     5641 tcagtatcta aaagaagtct atgatttgcc cgataaggct tttctgatag aatcgaagtc
     5701 tacaaattct ggagagaacg ccactttttc actggagatt cttcattcct taggagctgt
     5761 accagaaaaa atcttattga tgaatgatcc gacactgcaa cgcaggactc gagcaacttt
     5821 tgagaaagta tggcaaaatg aacaaactat tttcgcgaat gctgtgccat ttgtgccgga
     5881 aatccttcac ttttctgaag agatcatctt tacagcaaaa gaactgaatc atcaatggcc
     5941 taaggaatat ttccatgcgc tagtattagg agaaatggaa cgtctccatg acgatgagaa
     6001 cggctatggt ccgaaaggaa aagattttat tcctcacatt gatatatcag aggaagtttg
     6061 gtcttcttac accagaatta aagagtcaat aaaaacggat ttttctcgta cttgaccatt
     6121 ttagcaagcg aatcctcctt tccaaataaa tttggttgaa attttcaatc gtttataaga
     6181 taatgaggag cgtgttgaat gtaatagcta aaagaatgga cgctagataa gagaagagga
     6241 ttcaaatgga aaatcaaaat aaattgtatc attttgttgg tatcaaaggt tcaggaatga
     6301 gctccttggc tcttgtttta catgaacaag gtctaaatgt ccaaggatca gacattgaaa
     6361 aatatttctt tacacaaaga gatttagaaa aagcaaatat cacgattctg ccatttaacg
     6421 cagataatgt aaaaccaggg atgacgatca ttgcaggaaa tgcatttcct gattcacatg
     6481 aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat tcgctatcac gactttattg
     6541 gtcattttat ccaaaattat acgagtatcg ctgtaacagg ctctcacgga aaaacaagta
     6601 cgactggact gctctctcat gtattgagtg gtgtccgtcc aacaagttat ctaattggag
     6661 atggaacagg acatggtgat ccgcaagcgg aattcttttc atttgaggcc tgtgaatatc
     6721 gccgtcattt ccttgcctat tcaccagact atgcaatcat gaccaatatc gattttgatc
     6781 atccggatta ttatacaagt atcgacgatg tatttacagc tttccaaaca atggctggac
     6841 aagtgaaaaa agctattttt gcgtatggtg acgatgctta tttacgcaaa ttaaaagcaa
     6901 atgtgccgat ttattattat ggtgtcacag aaaacgatga tatccaagca cgaaacatcg
     6961 aacgcacaac ttctggttct gcttttgatg tctatcatgg ggatgaattt gttgggcact
     7021 tcactgtacc agcattcggt aaacacaata tcttaaatgc tttaggtgtt attgcagtag
     7081 cttatttcga aaaactggat ctgaaagaag ttgcagaaga aatgctgact ttcccaggag
     7141 taaaacgtcg attcagtgaa aagatcgtag cagacatgac cgtagtcgat gattatgcgc
     7201 atcaccctgc agagatcaaa gcaacgattg atggcgcacg ccaaaaatat ccagataaag
     7261 aaataattgc agttttccaa ccccatacgt ttacgcgtac gattgcattg atggatgaat
     7321 ttgcagaagc acttgattta gctgataaag tttacttatg cgatattttt ggttcggctc
     7381 gggaagaaca aggaaacgtt aagattgaag accttggagc gaaaatcaaa aaaggtggag
     7441 aagtcatcaa agaaaataat gtatctccat tgttagatta tcatgatgca gtagtaatct
     7501 tcatgggtgc tggagatgtt caaaaatttg aacaagcgta tgaaaaactt ttaagcagca
     7561 cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct tataaaaata gaaagagggg
     7621 gagtggcaaa aatcttgcca agcccccttt tttgcaactt atgataaacg gtgttcaaaa
     7681 gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca tgagaagctg ttttttcttg
     7741 gctgttctta ttatttgatg caggacagct ttttcacgac ctctgtaaac tataggaatc
     7801 tgttaaatca agggagcaga acttgccttt tacgctactt ttggtacaat aatgtatgat
     7861 taaaaataaa ctggagggtt tctatcttga acataggtaa gccgagaaaa ctaggaaaaa
     7921 caataaatga aatcgaggaa ggcgactcgt tatccttaac agaatctatt gaagataacc
     7981 agcttttgct gtatttggga ttgacgaatg atgcaaatcc tttgtatatc caacatgact
     8041 ttgcacaaaa aacagaatat caacagccaa tcgttccatc agtgatgctg atggggatca
     8101 tcacaagtgc aatttcaaag catcttcctg gaccaggatc acatgttgtc aatttttctg
     8161 tcaattttat tgaaccggtt ttccattatg aaacactgac ttttcagttt gaggtcataa
     8221 aagtagataa gatgaaggat gtggtgacga tttctgtaga agcggagaat acgaacgaag
     8281 atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca agttcctgaa ttggaaatag
     8341 caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta catcaatgtc aggtatcaat
     8401 cgtttttatg caaaagttta tggattttta ggtatgggac ttgcccttag tgcagtcact
     8461 tcttacttgg ctctttgggt atttcctttg caattagcaa cttttatcaa taattttcca
     8521 ctaggctttg tgggtctgtg gatcgttgaa ctgattttag taatggttct aggcgtaaaa
     8581 gcacaaaaga atccgagctt agcaatcggt ggatttattg cctactctct tttgaatggc
     8641 ataacattag cggttactct tgcatactac gatattggaa cagtaacgca agctttcttg
     8701 acagctactt tgatgttcat ggggatgtct gttgtcggag taacaacaaa aagagatctt
     8761 agcggggttg gaagagcagg acaggcagct ttaatcggag tgatcattgc actagtcttg
     8821 aacatcttca ttttgagaag tagcgtagtt gagctgttca tctccctact gttagtcgtc
     8881 atctttgcag ggattacagc ttatgataac caaagcatcc gtaatatcta tgtccagaca
     8941 aacggaacag cacatagtgg aattgctgta ttcatggcat tgaagctgta tttggacttt
     9001 atcaacttgt tcttggcgtt tctgagaatc tttggacgga acaactaaaa aagaaaaaaa
     9061 ttcatcagtt ggggctatac cgaattattt ggtatggtcc cgctttttct tttcgcattc
     9121 cgcaggattc tttgttagaa tgaataagaa ctttacagta aggagttaag aagatgacaa
     9181 aaaataaatt attactcgtt gatgggaaca gtgtggcttt tcgcgcattc tttgcgcttc
     9241 acaattctct tgaacgtttc aaaaatagaa acggtcttca tacaaatgca atctatgcat
     9301 tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc gactcatgtt ctggttgcgt
     9361 tcgatgcagg aaaaacgact tttcggacag aaatgtatgc ggagtacaaa ggtggaagat
     9421 ccaaaacacc tggcgaattc aaagaacaaa tgccatatat acgtgatttg ttgacaggac
     9481 taggggtaca gtattacgaa ttgcccaatt atgaagcaga tgatattatc ggtactttgg
     9541 cagaaaaagt agacaaagat caatttgatg tcgtcgtgct ttcaggtgat cgggatttga
     9601 ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt aaaaggtgtc agcgatatcg
     9661 aaagctatac accagaacat gtagcggaaa aatatgatgg gttgacgcca aaacagatta
     9721 ttgacatgaa gggactagcc ggagatgcct ctgacaatat ccccggtgtg actaaaatcg
     9781 gtgaaaaaac agctatcaag ctattgaaac aatatggctc agtcgaagga atttatgaac
     9841 atatcgatga aatgaagcaa agcaaaatga aagaaaatct gataaatgac aaagagcagg
     9901 ctttcttatc gaaaaaattg gcgaccatca atacttcagc gcctgtggat gtctctatcg
     9961 actcattgaa gtatgaaggg aaaaatctcg ataaactcgt tccgttttat aaagaaatgg
    10021 atttcaatca gttcctttct aaattgaata tcgtagaaga agaagtaaaa atggatgata
    10081 ttttgtttga agtcgttcat gaattcaaag aggaaatgtt tactacggat atggcgttgt
    10141 acgtggaaat gatgggcgac aattatcata cagaggaaat cgtcggagta gcgtggggga
    10201 cagaaaagaa aatttatgtc acgaacgatc tatctgtatt cgaaaaccga gcgttccatt
    10261 cttggatcac tgatccgact cggctgaaaa aagtatatga tgcgaaacga acttatgttg
    10321 ctttaaaccg ctatactgga aaaacgaaag gaatcgattt tgatgtcttg ctaggtgcat
    10381 accttctcga tacgaatgaa aaaagcacag atatcgaagg aatagctgct cattacggat
    10441 acaacgatat ccaatcagat gaagctgtat acggcaaagg ggcgaaaaaa ggtttaccag
    10501 aagaagagga actctttttt gctcatttag cgcgaaaagt agctgcaatc aatgcgttga
    10561 caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga agatcttttc cggaaaatgg
    10621 aactgccatt gtctatcatt ttggcagaga tggaaataag cgggatcaaa gtagatgcga
    10681 ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt aagagagatc gaacaaaaaa
    10741 tctacgaaga agccggcgaa gaattcaatt tgaattcacc aaagcaatta ggagtgatct
    10801 tatttgaaaa aatgggatta cccgttatca aaaaaacaaa aactggatat tccacagccg
    10861 tagatgtact ggaacagctt cgtgaacaag caccaatcgt agaggatatc ctcacctatc
    10921 gtcaaattgc aaaaattcaa tctacctatg tcgagggact gctgaaagtg atccaatcag
    10981 atggaaaagt gcatacccgt tatgtccaga cattgaccca aacaggacga ttaagctctg
    11041 ttgatcctaa tttgcagaac atcccgattc gattagatga agggagaaag atacgtcagg
    11101 catttgttcc tagagaaaaa gactggctga tttattcttc tgactattca caaatcgagt
    11161 tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa agctgccttc ctagaaggtc
    11221 aagatatcca tgccagcaca gcgatgcgtg tatttggaat cgaaaaggca gaggatgtca
    11281 caccaaatat gcgtaggcag gcaaaagcag tgaattttgg tatcgtgtat ggcatctctg
    11341 attacggact ttcacaaaat ctaggaatca gccgaaaagc agcacagcaa tacatcgata
    11401 cgtactttga aaaataccca ggtgtcaaag aatatatgga aagaatcgta cgcgaagcaa
    11461 aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg atatctgcca gacatcaatt
    11521 caagaaatta caatatccgt tcttttgcgg aacgaacagc tatcaataca ccaatccaag
    11581 gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt ggataaacga ttaaaagaaa
    11641 caggtcttca agctacgatg ctgttacaag ttcatgatga acttgtcttc gaggtaccgg
    11701 aaaaagaact tgaatcatta gataaattgg tgaaggaagt tatggaacaa gctgtttctc
    11761 ttcatgtacc actgatcact gacagcagtt ggggaaaaac gtggtacgaa gcaaaataaa
    11821 cattgtttaa gacagagcaa tataatgaag gtgaaaagaa aaaatgccag aattaccaga
    11881 agtagaaacc gttcgtaaag gattggaacg gttagtagta ggaaaaacga ttcaaaaagt
    11941 tcaggtcttg tggcccaaga tcatcgagca accagagaca cccatctttg aggcttctct
    12001 tgttggcgaa accatccaat ccattggacg tagagggaag tttttaattt ttcatctcga
    12061 tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa tatcaattta cgaaagaaaa
    12121 tacaccaatc gataaacata cgcatgtact tttctttttt gaggatggaa gccaacttcg
    12181 ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc gaaaaaggag caagtgcaac
    12241 ttaccgagga atcatgaaat tgggacctga gcctttgcca gatagcttct tattagcaga
    12301 tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag ccattgctgc tagaccaacg
    12361 tctagtgact ggtctaggta atatttatgt agatgaagca ttatgggaag cgaagatcca
    12421 tccagaacaa ccggcgaaca cactgcgccc caaagaggta gaacaattac gtctatcgat
    12481 cattgatgtt ttggatcgtg caatagaagc cggaggtacc acgattcgca gttatttgaa
    12541 tgcattagga gaatcaggcg gatttcaagt agcactgcat gtctatcagc aaactggcaa
    12601 accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa gtggcacaaa gggggacgca
    12661 ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag gatttgtttt aggtttgacg
    12721 ggtggaatag ctaccggtaa aagtacggca gccaaagtct ttcagtctca tggatttcct
    12781 ttgatcgatg gcgatgtgat tgccagagaa actgttgagc ctggaacgct tgctctgagg
    12841 aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg atgggcaact agatcgaggg
    12901 aaacttggga tgattgtttt tccatcaaag gaaaaacggc aaaaacttga tcagctatta
    12961 gatccgttta tccgaaaagc gataaaagat caaatcgcct ctctaagttc caatcatcct
    13021 ttagtaattg tagatatccc attgctctac gaaggtcatt atgaccacta tatggatgca
    13081 gtagctgtcg tttatactac gccagaaact cagcttcagc gtttgatgaa acgtaatcaa
    13141 ctgacgctag aacaagcgca gcagagaatt agtagtcaac ttccaatcga agaaaaaaag
    13201 cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag aaaaacttgt gggacaaatc
    13261 gaaaattggc tttttgctaa tcgctttctt tgagtatctg tgtcaatttt gctatcctga
    13321 gtatatagaa agtgagggat agcgatggga tcattaaaaa atcaaaagga taaagtcgta
    13381 ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag aaaatctaat cgatgaaggt
    13441 cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt accaagcaag aatcgatgaa
    13501 atagaagacc agcaggcaga agaagaagcg aagcggcaag atttgactca agaacgaaaa
    13561 aatccgacac ctgatcgtag taaaacaccg ctcccagaaa gtgaccacat tcccgatgag
    13621 caaaaaatcg aggaacagcg aatgaaacac tatacaggaa tcgaagagaa gttataaaat
    13681 agtaaaaaat cgagatgtga cttgaagcag atgagaaggt gttcatctgc ttcaagtttt
    13741 ttgtgctaga tgactaaatc agcttttgtg taagttattt ctcagtaatc aaggtgatca
    13801 gtattggaaa attgaaattt agtaaggaca ggggacacaa ttacgcatga aagtagaaaa
    13861 aattgttctt atatccgtgt tttttgggta ggacagtcgc gtttgtgatt tgtttcgtgt
    13921 tataatacaa ctagttgtaa agaaagacac atcctttata taaaatgcga tgcaagaggt
    13981 gagacaaaat gagatgtccc agatgtcaac ataataattc acgagtaatc gacagccgtc
    14041 aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg tgagaattgt tcgttccgat
    14101 tcactacatt tgaacgaatt gaagctgctc cgttattagt aatcaagaaa aacggggaac
    14161 gcgaagagtt caatcgcgat aaaattttaa gaggactcat ccgttcagca gaaaaaagac
    14221 ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt cgaaaaccgc gtacgcagtc
    14281 tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata tgtgatggaa gatttagttg
    14341 atttagacga aatcgcttat attcgttttg caagtgttta tcgccagttc aaagatatgt
    14401 ctgttttctt aaaagagttg caagatatcg tggataaagc aaaagaaaat aataaagaga
    14461 ataataaaga aaacaattaa gaaacaaaaa ggaggcattt ccttggaaaa tgcttggaaa
    14521 gagcttcaac caaaaaatat ttaccaagga agaaaagcgg ctccgctgag agaagacagc
    14581 aagaacgcct tgctgtatct ttatcagcca ctaatcggcg gagaagcctt gagtatctat
    14641 ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag gtcctgaagg gatgcatgca
    14701 gatctgctta gtagtctagg ctgcggtatc ccgcaatttt atgatgcaag aaaaaagcta
    14761 gaaggaatcg gcttactgga tgtttatttt aaagaagata gtgatctggg acctcgtttt
    14821 atttatgagc tgttagaacc actcattcct aaagcatttt tcaaagactc tgttctggct
    14881 tttcttcttc tggaaaaagt tggtgaacga cgattccaga agcttaccca attctttgaa
    14941 cctaaaaggc atacagcaga gggatatcaa aaaatcacaa aaaaattttt agatgtttat
    15001 cgcttcaatg aagcagtgta cgctgctaat caagaaaaca tcgaatcgct gaataaatct
    15061 tttgaagaat ctgaatcagt agcattactt gaagcagatt cgacattcga ttggcagttt
    15121 ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg atgatccaat catcaaacaa
    15181 gtcgacatgt atcatcagct ttacggtttc gatgaattga cacttggcga actgatcagt
    15241 caatcttatg actttagtga acaaaaagta tctgaaaaag aactgcaaaa agccattttg
    15301 gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag ttgcggtttc tgaaaacagt
    15361 aatgaaccct cgtcaaacga acatttgaca aacgcagcgc ttcagctgat aaaagaagca
    15421 cagagcattc caccgatgaa atatctggaa gcaatcaagt cagaaaaagg cggatatgtt
    15481 tctaaacaag aaaactggtt actgcaagat cttgtttctc aatcaggtct ttccagtagc
    15541 gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga ataatgctag cttaaatgcc
    15601 agttatgtaa atacagtggc aaatgaatgg gcacaaaaga aaatcgccac agcagaagaa
    15661 gcgatccagc acattcggca aataagtaat caagcaaaac aatcaaaaca aaagaagcaa
    15721 acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa agcttcctga ttgggtcaac
    15781 cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag cagagattga ccgccgtttt
    15841 aaagaatacc tggcaaaaaa ggagggtgat atctagtgga agacgtaggc aaagaattga
    15901 agaggatcat cagcaaacgc aattatcaag aaaagtatgc tgaaatgatc gctgaagtct
    15961 taaatgatgc ggatgttcaa gcttttctaa gcgcacacca agaagaattg acacaagcgg
    16021 atattgaaaa aagttacgct aaattgtatg aattcgtgca agaaaagcgt aaatatcaaa
    16081 aaaacgatcc aacgatgatc gctcccggct atgagccgaa gctgacgttg aattttcatt
    16141 atgtggatgt cacttatgta ccaacggaag cattgttagc acggcaaaga caagaagaaa
    16201 tcagaagccg agtaaaagca atggatatgc caaaagatat tcaatcggct tcttttgaaa
    16261 actttgaacg tacagatgga cgaggatatg cgagcttaga agccctagat tttgtagaga
    16321 aatatactgc tgatccaaaa gggtatcata aaggactgta tctagtagga agttttggta
    16381 ttggaaaaac atatttatta ggagcaacag cgagagaatt agcgattgct ggattcacca
    16441 cgacgctagt ccattttccg acatttgccg ttgagatgaa acaggcaatc ggaaaagatc
    16501 aagtggcaga aaagttagat gcagtgaaac gttcacctat tttgatgatc gatgacattg
    16561 gtgcagatgc gatgagtagt tggatacgag acgaagtatt cggtgttatc cttcaatatc
    16621 ggatgcagga acagttacca accttcttct catcgaattt tacaatggat gaattggaac
    16681 agcatttgtc cgtcactcaa cgtggagatg aagagccgct aaaagcgaaa cggataatgg
    16741 aacgcatacg ttatttgaca aaagaaatcg aaatgactgg tcgcaaccga cgcaatggtt
    16801 gaacagttga caagtccaac tgacatttga cctagtagta aaagcaggtc agcgaatcat
    16861 tatatggaca gaaatgaccg gagacaatca ccgcaacaag taatgcagaa acttaaacgg
    16921 aggatttttt atgagggcta ttttttctag tatatctgta gttggtctat tttttctttt
    16981 agcaggctgc ggtagcgcag atagcaacac aggttctgtt gattcgattg atgcgggtca
    17041 aacagtaaaa acaagcagta cgaaaacgga gagacaaagc atatttgtcg gaacgattga
    17101 aagtatcact caagtagaag cagaagaaga aacgcttcag ttgaaactcg tcgatgtcat
    17161 ggccaaagaa gatccagcga atatgggaac gtcattcagc aatgacggag tgatcttaaa
    17221 tgctactgct gaaaaaataa gcgtggacat aaatgaattg aaaatcggtg acaaagtcca
    17281 atttacatta gttgagatgc cgatcatgac gatgtctatc ccaccgcaag ttcctggaga
    17341 ttcgattatt gatgtttcat tgaacaaata accaataaca ttttttcaaa aataaga
//
LOCUS       DACDCJ010000062        16479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDCJ010000062 DACDCJ010000000
VERSION     DACDCJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.062"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(15..212)
                     /locus_tag="IV038_002025"
     CDS             complement(15..212)
                     /locus_tag="IV038_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8342226.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(606..2333)
                     /locus_tag="IV038_002026"
     CDS             complement(606..2333)
                     /locus_tag="IV038_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8342227.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2326..3519)
                     /locus_tag="IV038_002027"
     CDS             complement(2326..3519)
                     /locus_tag="IV038_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP8342228.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3706..4350)
                     /locus_tag="IV038_002028"
     CDS             complement(3706..4350)
                     /locus_tag="IV038_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8342229.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4444..4578)
                     /locus_tag="IV038_002029"
     CDS             complement(4444..4578)
                     /locus_tag="IV038_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP8342230.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4580..6712)
                     /gene="feoB"
                     /locus_tag="IV038_002030"
     CDS             complement(4580..6712)
                     /gene="feoB"
                     /locus_tag="IV038_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP8342231.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6709..7182)
                     /locus_tag="IV038_002031"
     CDS             complement(6709..7182)
                     /locus_tag="IV038_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP8342232.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7583..7807
                     /gene="nrdH"
                     /locus_tag="IV038_002032"
     CDS             7583..7807
                     /gene="nrdH"
                     /locus_tag="IV038_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP8342233.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7966..10125
                     /gene="nrdE"
                     /locus_tag="IV038_002033"
     CDS             7966..10125
                     /gene="nrdE"
                     /locus_tag="IV038_002033"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP8342234.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10175..11140
                     /gene="nrdF"
                     /locus_tag="IV038_002034"
     CDS             10175..11140
                     /gene="nrdF"
                     /locus_tag="IV038_002034"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP8342235.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11229..12368)
                     /locus_tag="IV038_002035"
     CDS             complement(11229..12368)
                     /locus_tag="IV038_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8342236.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12677..13390)
                     /locus_tag="IV038_002036"
     CDS             complement(12677..13390)
                     /locus_tag="IV038_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP8342237.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13644..14345)
                     /gene="nagB"
                     /locus_tag="IV038_002037"
     CDS             complement(13644..14345)
                     /gene="nagB"
                     /locus_tag="IV038_002037"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP8342238.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14579..16111
                     /locus_tag="IV038_002038"
     CDS             14579..16111
                     /locus_tag="IV038_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP8342239.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16302..>16479)
                     /locus_tag="IV038_002039"
     CDS             complement(16302..>16479)
                     /locus_tag="IV038_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8342240.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5108 a         3408 c         2860 g         5103 t
ORIGIN      
        1 catttttttt cattttagca cttccctgga attgctccac ctaaaaaacc acctatagac
       61 attcctgcaa tacaagtagt tgccttggcc caatcgaccg tacatttatt tttagtgcaa
      121 tacactccat ttccataata ttttccacta tgagtggtac ccccatagat aagttgtgtc
      181 tctttaatag acaaaatttt taaatgtttc ataatctaat cacccctaaa ttaatgtttt
      241 tagactatta ttctaacatt cattaaattt caaaaaacaa ttccttcttg aaacgtagca
      301 aaataattcc tgaataagaa tttaatcact tttaagtata taaactatgt atattcttta
      361 atattttttg agaatgcact tcgtacataa taaactttaa tcacattagt ctttcatacc
      421 tacgatcgat ctctttgact cgcacccata aattcataat agtttgtcgg cgtctttgaa
      481 cgtttcgaag atgagctacg cattctcttt ttttatagac aaaaaagata gagacgaaca
      541 tggagcttcc aaatttggat atttgatcca attttggaag ctccatttat ttcgtgtctg
      601 tctttttatt ttttccagct ttcgactaat tcttggttct ctttgatcca gtcttttgct
      661 gcttgctgtg gatcttttcc attgttgatt tccaacataa ctttttccat atctttttct
      721 gaccaatgga aattgtctaa tactttgtaa gcttctggct ggtcttcttt caatcctttt
      781 cgaaccatcg tatggatctg ctcttcgctt cccattccgt tttcaggatc ttctaagtat
      841 ttcagatcat atttggcaaa catccaatga ggtgtccatc ctgtaactac aattggttcg
      901 tgttttttga tcgcttgacc tagtgcaacg gtcattgctc ctgaagaaga tgtctctact
      961 ttccagtctt tcaagttgtc atatttttgg atcgtgtttt cagcagccgt aactacacca
     1021 gctcctggct cgattcctat gattttctta cctgcctgat cggttaactc atcgatcgaa
     1081 tttacatcca tgtaggctgg cactgctaaa ccaacttttg ctcctgtcaa attggcacct
     1141 aggtcttcga cttgatcttt atattgtgca tattgtgaac catgagtttt aggtagccaa
     1201 gctgaaacca tggcatctga ctcgccattt gaaacagatt tccacatgat tgaattatct
     1261 aatggcgtca tcgttacatc atagcccatt tgtttcaata cttcaccgac tacatttgtt
     1321 gaagcaactt ctgtatccca ttcaacataa gataagttga tacgttttgt ttctttggca
     1381 gatgaaaaac tccctatacc aatcgcacct aagatcacta ctactacagc accaatgatc
     1441 agtccttgac gttttttact tttctgtgtt gttgctcctg cagctttttt attgtttggc
     1501 tggttcaaat gctgtgtaaa acggtcaatg atgattgcta agataactaa tgcaaccccg
     1561 ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag
     1621 ccgggtgcac cgatcataga ggcaataacg accattgaaa gtgacagcat tgttgtttga
     1681 ttgatccctg ccatgatcgt acttttagct aaaggcaatt ctagtttgaa tagtttttga
     1741 cgacttgtgc tcccgaatga atcagaagct tctactaatt ctttaggaac ttgtcgaatc
     1801 cctaaattcg taaaacgaac ggttggtggc agagcaaaga tgactgaggc aaatacccct
     1861 ggaaccatcc cgataccgaa aaatgcaact gctggaatca aataaacgaa tcctggcatt
     1921 gtctgcataa agtcaaggat cggtttgata atactttggg ctttactgct ttttgccatc
     1981 aagatcccta atggtacccc aatgatgatc gagatcacac tagataaaag aacaagtgtg
     2041 acagtattca tcaaatcatt ccatagacct tggttataaa taaaccatag accgattaat
     2101 gtgaacaacg taagtcctgg acgtttctta gaaataaaga aagctgctac cgtcaataac
     2161 aagataaaca aaggagctgg aatcactaac agtaaatttg taatccccga catcactgcc
     2221 tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg
     2281 atattttcta cccagtttgc cactggtaat tgatagttca atagattatt catgttcact
     2341 cacctcgctt gtttctgcta atgcttcaat cacactacct cggatgacga caccttttaa
     2401 ttttccatcc tctacaactg caagtggagt cggtgagtca taaataatat tgaaaatatc
     2461 acttaccacc atgtctttcc caatcaccgt cacattttca tctaggaagt cgatcaatgg
     2521 acgtttttgc ttcctagctt ctaacgcttg atctgcagtg atgatccctt tcaggtgtct
     2581 ctttctgtca acagccatca gcatgctgac ttcttcatta cgcatccttg tcaatgcaac
     2641 gtttggtcca tcagattcga tattcgtcgt caatgcaggt accatgatgt tttgagccgt
     2701 aagcactttg gaacgatcga cttcttccac aaattcacgt acataatcat ttgctggatt
     2761 ggtcaatatt tcttcacctg ttccgatctg catgatttcg ccgtctttca tcaaggcgat
     2821 tctgtcccca atccgtaaag cttcattcaa atcatgggta atgaaaataa tcgttttttg
     2881 aacattggct tgcaggtcca gtaattcgtc ttgcatttct cgccggatca gcggatcaag
     2941 agccgagaac gcttcgtcca tcagcaagat ctcaggatca tttgctaaag ctctggctag
     3001 gccaacccgt tgttgcatcc caccagataa ctgactagga tattggtctt taaatgaaag
     3061 caaactagaa ttttctaatg ctttttctgc tttttcttgt cgttcttctt taggtacacc
     3121 acggacttct aacccgtatt ctgtattttc caagatcgtt cgatgtggaa acagaccgaa
     3181 gttttggaaa accatattga ttttatgacg acggacttct cgcaattctt ctttgcttaa
     3241 cttcgataca tcttctccat caatatagat actgcccgaa gttggctcaa tcagacgatt
     3301 caatagacgg atcaatgtgg atttcccact cccggaaagc cccatgatca cgaagatttc
     3361 tccttctttt acgtcaaaat tcacgtcata cacaccaacc gttgcaccgg ttttctttaa
     3421 aatctccgtt ttcggctggt gatcattcat catatctaaa gcggcttgtg tctttttacc
     3481 aaatatcttt gttaaatgtt ttacttctac ttttaccaaa caaattctcc ttttttatgt
     3541 aaattcattt ttctttctga aaaaagtgac cacactatgt taacatcatg tagtcacttt
     3601 gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa acttggctaa atctttgtaa
     3661 ataaaaggtt tagccaagtt aatatttttt taatatttca cacaatcatt ctgcaagctg
     3721 atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta tattcatttc caataaaatg
     3781 aacgatgtcc cctgccgtaa atttagcata ggggccaggt gaaacaagca gttcttcatt
     3841 atgagaaatt gcgataactg tggcgccagt cgcctgccag atattcaact cgctaattga
     3901 acggtccaga tgcattgctt tttctgataa agataattca aacggaagta aaggattcat
     3961 cttatgtatt cgcttcgttt gattgaccag ctgatccaat aattgagaaa aatgattcag
     4021 ctcatcttgt tgcttctcca cacttgccat gatttctgct tttgtatctt ggatggattg
     4081 tacatcttga tattgcaata ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc
     4141 acttccatgc cgtacttcca tgattccaag atctaccagc acattgatag ccttgcgggt
     4201 agtctcagga gaagcattgt acgtactggc aagtgttgag cgggcatgga tcttctcacc
     4261 cactttatat cggttttcaa cgatacgttc ggctaaatca acagcaatct gctggtagcg
     4321 cgggcggacg atttttttct ttccagacat tttctttcct ctttcttttt ttactttccc
     4381 tcatcttact atgctctagc aaaataagca aaaaagggga gaatagcctc ttgcaacgac
     4441 tgatcatcgc ttgatctgtt ccttttttac tgggcaagct gtttgacagt catcacaatt
     4501 cttaccggat tttaaacgag tatagacaat tgcgcctgca cttccaaaga ttaacacact
     4561 taataagaat gtcgccatcc tattcctctc cttctaatga caatatagta taaaactggt
     4621 tcttggattt tggctgacgg aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa
     4681 gatacgtccc taacgctacc tgtccaccct ccaataagtg tcccaactga taaatcacaa
     4741 aactaactaa ataagctaaa ccgcattgat agcctatggc gatccaagtc catttcttgg
     4801 cattcatttc ccgatggata gccccaattg ccgcaaaaca aggcgcacaa agtaaattga
     4861 aggctaacaa tgaataagct gctgcgggag tatatgtgtg ttgtagcact tgccagattt
     4921 ctctgccatt ttcggatact tccgcatgac caaacaaaat accaaatgtg ccaataatat
     4981 tttctttagc gaccaatcct gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa
     5041 gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc taagatactt tggtcttcag
     5101 aaacaggttg agcataaaaa ttataagaag agctgaacca gatcacgata ctagaaacaa
     5161 agataatcgt tcctgctttt ttaacaaaag acttgctgcg gtcataggtt tgtcttagga
     5221 ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat gataaaagga gctgtatctc
     5281 cagcaaacaa acgtgttttt ttcaagctga ttccagagaa aataattgca gtcataccaa
     5341 gaaaataggc agaaggagct acccaagatt gatgaggaaa aaaggcccct gctactaagg
     5401 caataatcgg caactttgca gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac
     5461 gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc tggcactcca catcctgtag
     5521 caatcagcat aggaatgaag gattttcctg aaagaccgaa tttcctgaaa agtcgatcca
     5581 tgacaaaagc gatcctcgcc atatatccgc agtcttccag aaagctcaag cataaaaaca
     5641 atataatcaa ttgcggtaga aatcctaaca ctgctcccac gcctgcgatg atgccgttca
     5701 agatcagctc ctgcatccat cccgctactt gccattcagc taacaatctg ccaacatgtt
     5761 ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc tgtccccatt gttcctactg
     5821 tttgaatagc cagataatag atcagccaca tcacaaatgc gaagatcggc aaggctaacc
     5881 aacgattcgt tgcaatctga tcgattttgt cactcatcga taattggaaa gaatcatttg
     5941 acacaacaca gagtgtacat agttgggtaa taaattcata acgttcattg accagaatcg
     6001 tctcactatc gtcgtccaat agtttctctg ttaactggat cagttgctcg atctcttttt
     6061 gctgagaaac tgctatctcg taatcctctc ttaccttctg atctcgttca aaatatttca
     6121 ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag
     6181 ctgcttctaa acgtggatca tatgtacagg gaacaggaat agaaggccgc ttttttgcag
     6241 actcctgaat cttttcgatt agtttttcga ttccttgctt tttcaatacg ctgattggta
     6301 aaactggtac accaagacca taggaaagct tttctagatt gatttttttt ccttcttttt
     6361 tgatgacatc catcatattc agaccaatga tcataggaat gtctaaatcc aataactgcg
     6421 tagttaaata aagatttcgt tctaaattgg tagcatcaat aatgttgatg atcagttcag
     6481 gctgttcctg aaccaaataa tctcttgcaa tcacttcttc tggtgtataa ggagacaaag
     6541 aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt ttttttcaac gatccttctt
     6601 ttttttctac tgttacgccc ggccaattcc ctacatattg attcgaacca gtcaagagat
     6661 tgaacaagct agttttcccg ctgtttggat ttccgactaa cgcgatattc attctttttc
     6721 ctccctttgt atcaagatta gttcggcctc tgactttcgt aacgtcagtt cataccctcg
     6781 cactgtcagt tcgattggat ctcccagagg ggctagtttt cggataacta tttttacatt
     6841 tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac
     6901 gacgatccca gtctctcctg cttttaattg atcaagagaa gtccagcttt cagaggaagc
     6961 agtcttttta ttgacaaaga tttgacctag tatttcccga cttactgcca ctcgatgact
     7021 ttttaccagt aaaatggcat tgttgcctga taaattgacg acgacgattt tacttccggc
     7081 aacgatcccg atattttgca aatggcgttt tgttttttct tctgtttcga ttcgttcgac
     7141 ttgataaacg aaattctgct ttgcctgcgc taaggtctgc atattgattc ctctcttgtg
     7201 aaataaatca tgtattaaaa tcattcatta tatgttctaa ttttcttacg tgttacgcaa
     7261 ataaatgaga atcattctca catagaatac tctcggtaat tgagaatgtc aattatactt
     7321 gtgatattat gagcgaatct ttagtattga tagataaaaa tgagaaaaaa atatttcttc
     7381 atagttttcc acaatttttt ctctattaaa tttgctattt tcccacacga tacttgtttt
     7441 cagctctttt ttcaaaaaca caaaacttag tattccacag tttccacaac acaatatgta
     7501 gtgtttgttg tttgactttt ttactaagag ttttatacta tctattatag ataataagaa
     7561 tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca
     7621 atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat
     7681 cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt
     7741 cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc
     7801 cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc
     7861 accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt
     7921 tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat
     7981 ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa
     8041 atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca
     8101 aatacgatga cttttccatc tattatggaa aaaatcaatt acttgatcga tcaagaatat
     8161 atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt
     8221 ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa
     8281 tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc
     8341 ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa
     8401 atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt
     8461 cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg
     8521 cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta
     8581 tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc
     8641 gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt
     8701 caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca
     8761 gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc
     8821 gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca
     8881 tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat
     8941 gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa
     9001 aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca
     9061 gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt
     9121 ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaaca
     9181 gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt
     9241 ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc
     9301 gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc
     9361 atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta
     9421 gactttacaa atatttactt catgctgttg aattattgga cacttgttga aagcaacaat
     9481 atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc
     9541 acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca
     9601 ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta
     9661 atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca
     9721 tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa
     9781 gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg
     9841 tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaacggca
     9901 caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa
     9961 ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga
    10021 cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat
    10081 gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt
    10141 cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat
    10201 aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca
    10261 attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc
    10321 tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt
    10381 ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc
    10441 cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt
    10501 tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc
    10561 ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga
    10621 aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc
    10681 tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat
    10741 ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga
    10801 gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct
    10861 ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga
    10921 agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc
    10981 actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg
    11041 cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga
    11101 agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa
    11161 atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt
    11221 ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca
    11281 tgttccattc ttctcaataa atagattttc cggatattta atattatcgt cttaatcact
    11341 gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc
    11401 aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg
    11461 atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga
    11521 aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca
    11581 tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg
    11641 aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg
    11701 ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga
    11761 atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata
    11821 aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg
    11881 atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat
    11941 aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc
    12001 aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga
    12061 attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt
    12121 acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa
    12181 acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaatt
    12241 gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg
    12301 ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt
    12361 gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt
    12421 tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag
    12481 tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc
    12541 ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga
    12601 aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt
    12661 tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt
    12721 gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat
    12781 agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga
    12841 ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga
    12901 taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga
    12961 gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg
    13021 cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt
    13081 gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa
    13141 tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc
    13201 ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa
    13261 ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg
    13321 ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca
    13381 tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa
    13441 aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt
    13501 tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat
    13561 atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa
    13621 cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca
    13681 acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca
    13741 atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta
    13801 cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta
    13861 tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt
    13921 ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag
    13981 atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc
    14041 agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat
    14101 tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca
    14161 tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat
    14221 aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct
    14281 tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt
    14341 tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt
    14401 tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga
    14461 atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag
    14521 caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat
    14581 gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg
    14641 tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg
    14701 taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca
    14761 ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt
    14821 aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat
    14881 tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg
    14941 gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga
    15001 gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag
    15061 tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct
    15121 gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt
    15181 tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca
    15241 gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct
    15301 aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc
    15361 tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg
    15421 atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc
    15481 agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc
    15541 agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt
    15601 gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac
    15661 tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga
    15721 taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc
    15781 actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact
    15841 agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg
    15901 ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct
    15961 cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca
    16021 gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt
    16081 gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga
    16141 gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga
    16201 ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct
    16261 tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga
    16321 tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc
    16381 ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa
    16441 ccagccgcac ctccaataaa gtacataaac ataaataca
//
LOCUS       DACDCJ010000063        16371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDCJ010000063 DACDCJ010000000
VERSION     DACDCJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.063"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..204)
                     /locus_tag="IV038_002040"
     CDS             complement(<1..204)
                     /locus_tag="IV038_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8342241.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTL"
     gene            complement(201..854)
                     /locus_tag="IV038_002041"
     CDS             complement(201..854)
                     /locus_tag="IV038_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8342242.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1142..2075
                     /locus_tag="IV038_002042"
                     /pseudo
     CDS             1142..2075
                     /locus_tag="IV038_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1983..2699)
                     /locus_tag="IV038_002043"
     CDS             complement(1983..2699)
                     /locus_tag="IV038_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP8342243.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2823..3008)
                     /locus_tag="IV038_002044"
     CDS             complement(2823..3008)
                     /locus_tag="IV038_002044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342244.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3235..4508
                     /locus_tag="IV038_002045"
                     /pseudo
     CDS             3235..4508
                     /locus_tag="IV038_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4615..5646)
                     /locus_tag="IV038_002046"
     CDS             complement(4615..5646)
                     /locus_tag="IV038_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8342245.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5653..6483)
                     /locus_tag="IV038_002047"
     CDS             complement(5653..6483)
                     /locus_tag="IV038_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8342246.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6480..7286)
                     /locus_tag="IV038_002048"
     CDS             complement(6480..7286)
                     /locus_tag="IV038_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP8342247.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7292..8116)
                     /locus_tag="IV038_002049"
     CDS             complement(7292..8116)
                     /locus_tag="IV038_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP8342248.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8106..9206)
                     /locus_tag="IV038_002050"
     CDS             complement(8106..9206)
                     /locus_tag="IV038_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP8342249.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9384..10169
                     /locus_tag="IV038_002051"
     CDS             9384..10169
                     /locus_tag="IV038_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP8342250.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10202..10585
                     /locus_tag="IV038_002052"
     CDS             10202..10585
                     /locus_tag="IV038_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP8342251.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10661..10792
                     /locus_tag="IV038_002053"
     CDS             10661..10792
                     /locus_tag="IV038_002053"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8342252.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10761..10997
                     /locus_tag="IV038_002054"
     CDS             10761..10997
                     /locus_tag="IV038_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP8342253.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11075..12046)
                     /locus_tag="IV038_002055"
     CDS             complement(11075..12046)
                     /locus_tag="IV038_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP8342254.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            12698..14320
                     /locus_tag="IV038_002056"
     CDS             12698..14320
                     /locus_tag="IV038_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP8342255.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRQFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(14606..15982)
                     /locus_tag="IV038_002057"
     CDS             complement(14606..15982)
                     /locus_tag="IV038_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8342256.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(15982..>16371)
                     /locus_tag="IV038_002058"
     CDS             complement(15982..>16371)
                     /locus_tag="IV038_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8342257.1"
                     /translation="NLIDYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGG
                     QQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKE
                     VADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT         4958 a         3539 c         2893 g         4981 t
ORIGIN      
        1 caaagtttgt tctatatatt ttatttcttt tccattcata ttactagaaa tttcatttgc
       61 catattatga atcttttctt ttctcgtctc caaatatgtt ttaggaaaga taaaaaaaat
      121 taatgaatga actaatataa ttattattcc aagaacagaa aatatttgta tgaacatttt
      181 tggaaatatc tttaattttt tcattttctc tccaatttat atcctacatt tcttatagta
      241 gttatacagt ctaattgtaa tttttttcta agttctttta tatatacatc tattactcga
      301 tcataaggag tttcttcgct atctttccat acataatcaa taatttgcat tcttgttaac
      361 acttgtccat tattatccaa taaacatttt aatacttcta gttcttttgc atttacatct
      421 atttcttcac catttatttt agctgtatag ctattaaaat taaccgatag atccttatat
      481 tcaaacttct ctaaatgtcc ataattcttc ttaattaaag aatctattct agcttttaag
      541 accggcaatg aaaatggctt ttctacatat ccgtctacta aattagtaaa tgcatcaatt
      601 ttatattctt catcactaaa tgcagtcaag attagaattg gtaaattgct tttctttcta
      661 atttctttca acacttctaa accatttata aaaggtatct gaatgtctaa gataaccaaa
      721 tttatatcgc tattaaattt tgacaaggct tctcttccat ctttagcttg aataacagta
      781 tatccaaatt ctgaaagata ttcacttatt ccctctctta tcatattatc atcttcaaca
      841 gttaatattt tcatatatac ctccacttaa gcctatacac tagatattat acagcatatt
      901 atggctctat aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttt
      961 taataaatgc aaacagacac cttgaaatcc tttagaaaaa aggcttctat aatattttgt
     1021 gttgttggaa aatcgaaggc gattttctac taacactttt tcttcgtttc aactcaaaaa
     1081 gagactcact tttggtaaaa tttagtcgac caaaacatca ccctaccaaa ggagtcgctc
     1141 tatggataat catactagaa aattactcgg attaacagat aaacatttat tttttgatga
     1201 agaatggctg attgaaaaag aatataaagg cgttcaagcg cagtttatta aagggagatt
     1261 ggatgactca ccttcccatt gtgaacactg tggatcgatt cgtattatcc gcaacggttc
     1321 ctatacgaca cgtactcaga tgttgaaagt caaagaaaag ttgactattt tagaattaaa
     1381 acgtacccgt tttctatgct atgattgtgg gcagaccttt tctgcgaaaa cggacttagt
     1441 tgatgaacat catcaactaa caaaggaact gaagcaggcg attctcatag aactgtacga
     1501 aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc tcggatggga cggtgacacg
     1561 gatcttgcgt gaagcaacga agcattacca gccacgtatg aacttccttc cgacggtgtt
     1621 gtgtatggat gagttcaaat caatgaagtc tgtttctagt tccatgagtt ttatttgtgt
     1681 tgatgggaca acccatcagc tatttacgat ccttgaagat cgcagacttt acaaactaac
     1741 tcaatacttc ctgcactttc ctagaaaagc ccgtttaaag gtcaagtacc tagaatgcca
     1801 gctattgtca gctgcttaaa acggtctttc caaatgcgga gatcgtaact gatcgctttc
     1861 atattgtcca acatatcaat cgcagcttca atcaattgcg tatccagatc atgaatcgtt
     1921 ttcgagcctc gcactctgaa aatcagaaga agtaccgtcg gctcaagcga tattggaaac
     1981 tcttactcaa agattccacg acattggagc cgctcaaacg gcattatcat cgactgttta
     2041 aacgaccaat tagtcaaaca gaaatcgtcg atgaaccgtt gtgcacaatg gtgtcctgaa
     2101 cgtacagcga caccttccat atccagagca gtcgccacat cacggggatg gatgccatcc
     2161 aaattaaaag caaccacgcc agtatgatgt tcaggatctt gcggtccgta aatggtgata
     2221 ccgccgatcg cttgtaattt aggtaaaacg taggcaacca gttcttgctc ataacgatga
     2281 atattctcca taccaatctc attcaagtaa tcgattgctc ctgcaatgtt cggagtacca
     2341 gcttcaaatt tccatggcag ctctttccat gaactatcgt aaagattgac aaaatctatc
     2401 atttcaccgc cgaattcgac tggctccatt tgttctaaca attgacgttt gccatataat
     2461 acaccgattc ctgtcggccc gcacatttta tgcccgctaa aggcgaaaaa atctgcatcc
     2521 aacgcttgta catcaactgt catatgcggt gctgcttggg caccgactgg actatcctca
     2581 actacaacag cctgttcaac cctcagccgt ttcaagcaca acaaataacc ttctggatct
     2641 ggtttgccga cctgaagatc atcgatcgtc acaaaggaat caattttttc cttaatcatc
     2701 ggatgtgctt caaaaaaata gtctagctcc cgcagttcag aattgagact actccgattt
     2761 tccagtgctg ctgatccgct gctcaaaatg ttcagcatcc actggtctcc caaccgcttg
     2821 acttatccct tcataaaagg acagttctgc tgccgtccca acaccttgtt gatagtctgc
     2881 aaaaggaatt ttatagccga actgctccag taaataggct tcagtaaata gtgatgaata
     2941 ctctcgtaaa acatccattc actgtcaatc atcgtcccat caaaatcaaa aataagtgct
     3001 ttggtcatct tctttccccc ttacccaaaa caagcaaaag ctgtgtcctg ctctctctac
     3061 agtatcagca taaaaactgc cgtctgaaaa gtcaaaggtg aaagaaatgt ggcgctataa
     3121 tttctaggac tgatagtaaa tcattctatc agtccttttg agcataaaaa aacaaaacat
     3181 acagaagctc cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct
     3241 gatcttcatc ttatcaaact attactagga atcaaagatg gaaacattca aatcactcat
     3301 gtcgagaaga aaaaaataca ttcaatcaac agtatcgttg tttctgccac gctcattaaa
     3361 acgattcagc cctgtcctca gtgtaaacag gtgaatcaac atgggctgat cgttaagaac
     3421 ggaaagaaaa aatcgctggt ccttctcaat aagtgtgcga atcaacggac ttatcttgct
     3481 ttggcaaagc aacggtacct ctgtcgtggc tgtcacacct attttacggc gaatacctac
     3541 attgttgaaa aaaactgttt tatcgccaaa caagtacatt ataaaatatt ggaggaattg
     3601 actgaaaaac aagcaatgac gacgatttct aaacattgcg gcgtttcttg gtcaaccgtt
     3661 tcgcgaacac tcgcttctct gctccccatg acaaacgtga aaataaattg gttgcctcgc
     3721 tgttcattgg tagatgaatt tcgttcattg aaaaatcaag tcggtcccta ttctttttct
     3781 tgtatggatg gcgatactgg gaaattactg gatattcttc cttcacgtaa gaagaaagat
     3841 ctcatttctt attttatgca atttgaacga cgtgcacgtt taaacgtaaa aatactcgta
     3901 acagatatga acgcctctta tgctttgctg ataaaagagt gctttcctca tgccaaactc
     3961 gttgtggatc gctttcacat cgtcaagcac ctcattcgca gttttaagga tatttgacta
     4021 tgagtcatga aaagcttcga ccgaaacgat ccaatacaag ccaaacacta tcgacaagtg
     4081 aaggctttat ctcgacttct gatcaaacga caggacacgc ttgtttatga taaatggaca
     4141 aaatggcgta attttggttg ggcctattta accgaaagtg aagtcgtaga acgtctactt
     4201 tctacttctg atgaactacg aatagcttat gcttactatc aagaaattct tcaagctttt
     4261 tatgacaaag aagcggacat cttctttcag cttgtaaaaa caatgcccaa gtcggttcca
     4321 agagaactgc atcatataaa aaaagcgttt ataaactatg aatccggcat tcgtttagcc
     4381 ttagagttgc cttactcaaa tggcaaaata gaaaatctgc atacccacat caaagcattg
     4441 aaacgaatag cttacgggtt aggatgagaa ccagaatttt cttaatcaat catctgatca
     4501 cttattaaac ataaaaaaac atctaaagca gaaaatcaat tctaccttag atgttcttat
     4561 atcactctat cagtcctatt tgacaaagag ccagaaatgt ttgccgacta tacactagct
     4621 ttgttgtgca aaatgctgag tatcaatttc cacagggatt gctagaatat ctttcagctg
     4681 aatgtataag gcatcgctaa ttttctcttc ccgatcgttc aaccgatcca ctaaaatttc
     4741 tttgccctcc agcgtttgaa aaacataccg ttgaatgttg cctaaaataa atgattcctt
     4801 ccatagaacc ggaatcttaa ccccctgagg aaccggttca aattgaatca gctccggtct
     4861 taaataataa atgcagcgat tgcccttagc ggaaaactcg tgggtatact gattcagctc
     4921 ataccattcg aaacgattat agttgccgat aaattccgca acaaatcggc tctgaggatt
     4981 ctgatacaat tccttcgagg tggaaacctg agccacccgc ccagcttcca tcacaaaaat
     5041 ccgatcactg attcgcatcg cctcttcttg gtcatgagtc acaaaaatca tcgtcatccc
     5101 tagcgattgc tggatagcgc gcaaatcccg ctgcagctgt tttctaattc gggcatccaa
     5161 agcacttaat ggctcatcca gcagcaggac tttcggttca gtgaccaatg agcgagccag
     5221 tgccacccgt tgtcgttgac cgccagaaag aaactggaca tttttatctg cctgctccgt
     5281 catatccacc aatgatagca tctcaaaaac cttttcctga atcacctcag gactcagctt
     5341 ctttaccttc aaaccaaagg ctacattttc aaaaacagtc atagttggaa acaatgcata
     5401 ctgttgaaaa acaaagccga tattgcgatc cttcgtaggc ttatcggcaa tattctcatt
     5461 gtccagcaca atctcaccgc tctttggttg ttccaatccc gtgatacagc gcagcaacgt
     5521 agactttccg cacccgctgg gacctagtaa agtaaccagc tcaccctttt caatctgaat
     5581 ttcatcaata gctaaaatct gtttttcatc aaaaaacaga ttgagatcat gtatttctaa
     5641 aaacattttc cgtcacaact cctttaaggg cttctttttc gactgcttct cttccctcgt
     5701 ctgcttaagg gacagctgtt gcttttgtcg ataagtcagc ataaaggtag ttaaagcaat
     5761 tcccaccata aacagcaaat acaaaaccat cacagcgcta gccaaatggc cgttttcgtt
     5821 catccgccgc agcatataga tgcgtaccgt ctgaaatcga ccgccaatca acaggttcgt
     5881 cagcatgtac tccccaaaaa ccgaagaaaa aatcatcaga gaaactaggg tcacaccgat
     5941 acggatgttt ggaaacagca cctggataat aatcgtagac atcggggcgc ccaaggtttc
     6001 tgctgcatcc actaattcct tcagattaat taagcgcatc tggttgttaa gactaagata
     6061 gacaatcggc aacgccgtaa taaacaatgc acctaccaac actacaaaca ttgggatacc
     6121 agtttcagca tacgttcgaa gcaaagccgt taccaaaatc actcctggta aagcatacgg
     6181 cagcaggagc agcttttcca gccaacgatt caaacgaggg aaatacaaat gaatccaaat
     6241 aatggtggga atcatcaaca acagaatcgt caccaagacc acacctgcta ataaaagtga
     6301 gcgacccacc gcagcaataa attccctgtc cgtcagcagc tgttggaacc attgcaaggt
     6361 aaaatcacta ggtaaaagcg attttgacca tttagtggag ctggcataca aaatcgttcc
     6421 aataatcggc agcaagtaat acaggacaaa gcagaacaaa atcggtttta ttaacttttt
     6481 cacgtctgca ccaccttgcc tttcttttga ataatcaaca ttgccgttac cagcaggaca
     6541 cctaaagcga ctgccaacgc actgcctaaa ttaggcttgg cgaacacatc tcccgacact
     6601 aaagccgcaa ttcgaatcgt taaaagattt acattactgc tggtcagggc atacgctgtt
     6661 tcataggtcc ccatgccatt agcaaataag atgacaaagg tgctggcaat cgatggtcgc
     6721 aaaaaaggca aagccacctt gaaccaagca tacgctgatg aagcgcccag caattgtgcg
     6781 gactccagcc attccttttt cacttccttc atgctgggat acaaaaacaa gacccctaac
     6841 ggaatttgga aataggtata cgtgatggtc aatccccacc aagaatacaa attgaagtcc
     6901 gctagaaatg gcaaaatcat tttcagcaca cctacattcc ccaacaaaat aataaaagag
     6961 aatgccagcg gaatcccagc aaaattagct gccaaattgg aaacaaaggt gattttctcc
     7021 tgcaatttag tcgatagatt cattaagcat aaacataaaa taaacgtccc gattaaccca
     7081 atcacactgg agctgacagc aatcagtacg ctgttaacaa aggcctgtgt gtaatatttg
     7141 ttgctgaaaa tatcactgta attggccaat gtccactggt tagttaaatc actgtgcaag
     7201 ctgcacagaa ccataaaaag cagcggcagc agtaaaaatg cacagaagat aaaggaggca
     7261 ggaaccagaa ccaagcgttt tttcatctct ttcatcccct catcaattct tgaatgtcct
     7321 gcattttctc tgacggtgca attcctaata accagcaaaa caatggagcc gccagcagct
     7381 gtggtacagt ttgttccttc agcttttggc gtttatttga aatcaaataa aaaggaactt
     7441 cacgatgtgc agccaagctg ccgccatgca gtccgtgcgc atccatcccg tgatccgcag
     7501 tcaccacgac ttgataacct ttttgcagcc attcaggaag gtaaatagcc aataatctgt
     7561 ccgcctgatt aaccgctgct gcatattcat gagaatcggc ggtaaatttg tgaccgacat
     7621 catcaatatt catcgaatgt acgaccaaaa agtctggctg atactgattc actaaataat
     7681 gggcatccgc aaataaatgc gaatcagggt aagtgtcctc actataaaag atgccatgct
     7741 gaatcagtcg ctcctcctga tgctggatgc gatcaaccaa acgattatag ggggcttgat
     7801 tgtacaactc gctgtaccag tggtaagcgg cggctcctgt ttttccgcca gcttccttaa
     7861 ctaattcaaa cagcgaccac tctttggatc gctgagcatg gtaattacta tagatgccat
     7921 tttcataggc tggaacgccg gtcatcagta cttcgtataa cggacgtgaa ttgctgggca
     7981 gctcactttt taccttcaat aagcttgctt gctggtgctc aactaaatga ttcatgaacc
     8041 caagctgggc gacggctgtg tcgtagcgac agccgtctag tataactaca atcagtttgt
     8101 caagtctact ttgcataggt cagtacctcc gcctgccaaa gctctggaag ctctacagag
     8161 gtcttgttcc aagcttcatt gtcttcaact ggcaccgccg cttcataagc atcctccggc
     8221 agtaattttt cagctacttc tttaggcagc tctaccttgc gaataggtcg cgcatagccc
     8281 tttgccaaat tgatttgccc ttcatcagat aaaatatatt cccgtgctag ctttgccgca
     8341 ttgggatgag gggcattttt attgattaag gttgtatacc cgctaatgat ggagccgtct
     8401 gttggcacag ttacctcaaa acgctcggag tcaatttggt cacgataatt tagtccattg
     8461 aagtcccaaa caactgccac atcgatttca cctttttcaa gatttgctac gctggcatca
     8521 acggtggaca aacggccttt ttctgccatt tttttgtagt aattcagacc tggctgaata
     8581 tccttttcgt cacctccgtt ggcaatcgcc gcagccaata cagcaaattg tgcttggttg
     8641 gcaatcgtca catcccccac gctaacttga tagtcaccct cttgcagctc cttccagcta
     8701 gtcggtgctt gcttcacatt ctctttatcg gtgataaagg aaatggttcc cgtatagctt
     8761 aacaaccaat ccccatcatc atcctttgcc caatccggaa tttcctccca atagttggtc
     8821 ttataaggta aggttaactc tttgctttca gccaacggac cgaagctgat accgacatca
     8881 ccaatatcgg cggttccatt ttttccttct gattcaaact tagccaattc ttctgcactg
     8941 gacatatccg tatccacgtg acttaagccg tattcttttt caaggtcgtt ccaagtgcct
     9001 acccaatttg cccaagtatc cggcattccc aagctggcaa tctctccttc ttccttggct
     9061 ttttctataa tcgtatctaa agagtcgtct gccgcagccg attgcttcgt tgcttcacct
     9121 gagccgcagc ctgtcagcag gatacttact gttgctgcac aaaataaacc gatagtcatg
     9181 ttacgaattt tttcattcat tttcattcat tcttctcccc tttcgataat tccattctaa
     9241 caaagctttg taatgagaga atcagtttct tgaaaaggtt tcatgaacaa tgtaaaggtt
     9301 gtatatggtt tgtaaatttc gacgaattcc cgttaacctt ctaaagcttt tgcggtatga
     9361 tagtagaaaa aaggaggctc cctatgcagt attatgatca gcatctccac acgtattttt
     9421 cctttgattc agaggaaaaa tttgaaaact atttagccta tcagccggag tttttcgttt
     9481 ctacggatca ctttgattta aacaatccat ctaccggaaa agatgacatt ccggattatc
     9541 cagcctatat aaacaaactg gactctttaa aagaaaccta ttccaccaaa tttctgcgag
     9601 gtatcgagat tggcgttgtt ccaggacaag aagagcggat cgtggattac ctcgctcagc
     9661 atccttatga tttaaagctg gtaagtattc atcaaaatgg ccgctttgat tatatggacg
     9721 atgtggtttt aaccaaagat cccattcaaa ctaccaagga atattttgat cagatgttgc
     9781 aggtcttgac ggattttcca gaaggagata ttttgactca ctttgattat ggattacgcc
     9841 gcttcacctt cacagcggaa gaattcgcgg agcattttga ggctcagctg ctaacgattt
     9901 tcaaaaaggt tgttgaacga gaattggcga tggagttaaa tgccaagagc ttcttgaaat
     9961 acggcaacga agacctttac cgttatgccg ttcctttgta tcaaagtgtc ggcggaaagc
    10021 tctttacctt aggctcggat gctcatgttg ccgaagatta tcagctgggc tttaaaaaaa
    10081 tgagtcagct gttggcagac tgtcaggtag aaaagttgct agtcattcaa ggaaaagaac
    10141 gccgcctgac gccgctgccg attttgtgat tcctatataa tagaagaaag tggtgaaaaa
    10201 aatgagcaac ctgtgggact acaatcaaga ggcacccatc cattatttaa ttgcgcgaca
    10261 ttgggatgcg ttgaaaattg aggcggtttg ccgatccttg ttagcagccg ttccaaaaca
    10321 acaactggaa aattttttgg tggctgacag cttgcagcgg gaaaaagttc aggcttattt
    10381 tgcagccttt aaagatcagc ctttggaata tttgcatgcg cagtttcatc ttttttatca
    10441 agtagccgcc ccagatgact acaacgattt acgaggacag ctgcaattga cctttcaagc
    10501 ggatgaaacc gcctataccg ttttattggg aatggctcgc cttggtgatc aggcgaaggt
    10561 cgagtggcgg attttcgata tttgatggca ggatcaaggc gtttcagttg caaaccttcc
    10621 tctctttcaa ttagaaaaac cgcagcagga agaagtttgt gtgctggtca ccgatgctgg
    10681 tgaaattcgt ctccgcttat ttcctgacca agctcccctg gctgtagcca actggcgctc
    10741 cctagctgag caaggcgttt atgacaacac acctttcggc tcgtgtcatt aaggaatttg
    10801 ttattcaagg tggcgctttg gatggttctg gtgatgaagc tgtttccagc tatgagggct
    10861 ttttcgctga tgaagtgcat cgcggacttt atcattttaa tggcgccttg gccttagggg
    10921 accacggccc ccacaccaac ggcaaccagt tttttattgt gcaaaacaca aaggcgcagg
    10981 ctgacctgct aatgtaaagc aagcatactt ggagcttgga ggactaccgg agctggatgg
    11041 tcgttatact gtttttggac aggtatacca agggttagaa atcgtagcag cgatcgcagc
    11101 tcagccgaca aacagtgaag atgtaccgat tgagccagtg ttcattcgac aaattaactt
    11161 cgaaaaaaca tcgtaaaaag acactcactg acttcagcaa aagctctatt cttgagctgg
    11221 ctgcagctag tgagtgtctt tcatttatcc ataagataaa gcttggattc aataaataaa
    11281 taaatgtttt ggttaaagct gtgaccagca aatgaggaac cgtgcgagcc agttgatctg
    11341 ccaatgcaaa catttcttta ggtgaaggcg ctggcgtgtc tgccaacgga tactccagtc
    11401 ccaatgcttg gtaaccgtgc ataccatagc agtggtacgg tagtaattct aacggtacga
    11461 accgatcaag atctgccaca aaacaagcaa tcgtcagcag ctcggtcagg ttcatattga
    11521 tacctggaac cacaggaact cgaattcgca cttcgcagtt aggatactca attaaaaagc
    11581 gacgaatatt cttcaaaatt tgttggttat ctaagccggt ccattgtttg tggagcttgc
    11641 tggtaaacat ttttacatcc acaaacaatg tatccacata gggagctacc ttttggattt
    11701 cattataagc accacaaaag ctggtttcca gtacggtatt gattccaatg tatttcgatt
    11761 tttgcaaaat ttctttagca aaatccgcct gaaccagtgc ctcgcctccg ctaatggtca
    11821 ctccgccatc cgagtgaaag taaaaaacag catccttctc aatctcatcc attacctctt
    11881 cagcggtcat ggtctggcca aatccttttt caatctgctt gctttgtgat tcgggaatcg
    11941 aacaccaggc acagcgtaat gggcagccct ttaaataaac aacggtccgt aaaccagcac
    12001 catcatgtag agaaccctgt tctattctta atactgttcc ttccatatag atttttgaat
    12061 tacacactag tttaaactaa aacacacaag gaaaaactta ccatttaatc ataaaattag
    12121 ctcatataaa tatactagga tgtaaaatag actggtcaaa atctggtcaa aaaagaggac
    12181 taatacaggt agtcctcttt tttgttattt taatattcta aatcattttt tattggttta
    12241 ttcccttatt cgatgtagac tcaaatcact atatcactag atatcaaacg gcgtcatacg
    12301 gaatcaaaaa catcaaattt gacctgttaa atggtcattg aataacattc aaaatcaata
    12361 aaacggagtc agacacggag tcaaaaacgc tgagagtaaa aaatatatac agccttgtag
    12421 gtaaagtacc acagggctgt atatttttta tttcagatta ccttaaatga ttccatttac
    12481 tttttatctc attttttcct actagttagt atcaaatact ctcctatttt ctcaataaac
    12541 tatttttgga tacatggtat ctgaaacagc taaaaccttg tcattacagc aaaaaatcac
    12601 gttatccaga ctgtagacaa agtccatttt atggatgatg tctacagtct tttcttttat
    12661 aatagattta tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa
    12721 tggtagaaat caatttgcga tgcttacgat tgatgatcta gtttctcaag atcatctcgt
    12781 tcgaaagatt gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta
    12841 ctccgattta ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca
    12901 gtatttattc ggtatccgtt ccatgcgaca aaccattaag gaagtcgata ccaatgtcgc
    12961 ttaccgctgg tttttaggct actccttcga ggagaaaatc ccccatttct caacttttgg
    13021 aaagaattat gttcgccaat ttagagaaac gacggtgttt gaggatattt ttgcctatat
    13081 tttagaacaa gcagttaaag caggttttgt aacagaggat aatttgtatc tggattctac
    13141 ccacatcaaa gctaacgcca ataaacataa gtttacgaaa gaaatgaccc atgatgaagc
    13201 gaaagcctat caggatgaat tagaagatga gatcaatcgt caacgaatag aggctggaaa
    13261 acgccctttt actttggact tagaaaagga agtgaagctg aaagaaagaa aaataagtaa
    13321 agctgatccg gaaagcggct actacgtcaa aggcgaacga gaaaaacaat ttgcttattc
    13381 tgcccatact tcatgcgacg acaatggatt tattcttagc acaatcatca ctcctggaaa
    13441 tattcatgac agtcaagtcg cctttcagct cgtcaaacaa tcgaagagac tttttcctga
    13501 aatcaattgt gttgtcgctg atgctgggta taagacacct aagtttgttc actttttgac
    13561 tcatttgaac cttcgacctt gtttgcctta ttcaaggccc aaagggaaaa aggggctatt
    13621 atcaaaaaat gagtttctct atgacgagta ttttgattgt tatatttgtc cgcaagatca
    13681 aatgctcgct ttttcaacgg ttactcgaga aaggtaccga gaatacaaat ccaatccaaa
    13741 agaatgtgtg aactgtcctc tgctaaacca atgtaccaaa agtaaaaatc atcaacgagt
    13801 catcacgaga catgtttggg gagatctgat ggatgaagtg gaacatcttc gcctaacaga
    13861 tctgaataaa tcaatttaca aaaaacggaa acaaacgatt gaacgaatat ttgcggatgc
    13921 aaaagaaaag catgggatgc gttggaccaa gtatcgaggc ttggaaaaag tcgcgacgca
    13981 tacgatgcta gtgtttgctg caatgaattt aaagaaatta gccacctggc tatggaaagg
    14041 caaggagcct ttattttttt gttcaaaaat tcgaaacgaa gtagataaaa agctgttcca
    14101 agctcgagtt acgagtttgg aacagctttt gtctacagtc tgcgatgtcg gagacatctc
    14161 gttttctatt aggaatcaac tcaggttcaa aagaagcctc ccggtctctg ggaacatcaa
    14221 tgtttacttt accaaagctc gttttaagcg tcttattgcc ataaccgttt cgtcgattgt
    14281 catgtttctt tggctcttta gaatgggcgt cataccctaa atggttattc aattctcctt
    14341 gaagcatttt ttcaaataag ggaccaaaca catcttttaa aacatcttgc atatcctcta
    14401 cagattcggg ttgataagta ttcaaaatgg attcagctaa cttctcagca tcaggatttc
    14461 ctttctttct agccatcgtg taatcacctc ttgatcttat tttagaaaaa taaaaaccgt
    14521 gaagcaacca cccgcctagg attgatggtt acttacacgg tttatattac actctcattt
    14581 ttggtgctaa aaagcaccga aaattttaat tattgtcaat taataattct tttggattcc
    14641 ttcttaatat attgaatgaa gaaataatca atgatacaag aagtactaaa ctcataaata
    14701 taactacata aatcatgaat ttaggtaata cattgatatc taaacttgat aatgtcttgt
    14761 taaatccttc agcttctgcc ccaccaccta attggctaga tgcagattgt ttagctattt
    14821 gtttagctat atcaccagta accttattca atatattatt cccaagcttg tcagctgtat
    14881 attgagctaa gaaataggaa ccaacgaatg cggggattga tataaatacc atctcaatta
    14941 ggaattgacc aaaaatttct aattttgata tacctaatga tagtaatact gctatttctt
    15001 tctttctagc attcatccat aagaataata ataatgaaac tacaactcct gcaaatatta
    15061 atgagccaaa aaataattta tttgaaattg agtaaatacc tgatatagat tgttgtaatg
    15121 cagggtaatt tgatgaactt ttaatcaaat tatattctct ccaatttata tctaatttcc
    15181 caagatcttt tattacacta tcaagattct tatcaccttt tacaaagaat gttgcatctt
    15241 ggtatacagc tgtatcttcg gtattaccat aaactttagc agctgtatga atatcagtga
    15301 ttaatgtatt ttcgtatagt tcttgtgctg cacttactac accgctatta tgcccatcga
    15361 atagaccttt aatttctact tctactgttt catctgcacc tttctcatta tcagcatcaa
    15421 ataaattaga ttttattttt atcttatctc caactttaag gttatttttt tcagctaaat
    15481 ctttatgcat taagatttta tttttatctt cattttctaa atgctttcct tctactaatt
    15541 tatatgcttc tgatacaaat tttgtttctt ttgttgagtc gttaacccca gttaacataa
    15601 ctgttctttt aaaattcttt gctctttcag gtgattgatt cgcaagagtc tcttgagttt
    15661 caataatatc ataatcaact aaatctgcaa cactgtttat tctttttaca taggagtcta
    15721 tactatctgt ttgagatatc tttttaatat cttcaccttt tacatttccc ccacctctag
    15781 gtgttcctgg atttacttgt ctatttatct ccatagaaaa actatttgtt atattagcaa
    15841 atgtttcttt tgaagctctg tccgtagcat ctttaataga taaactaatg agacttaaag
    15901 ttgacataga taaaataact aatatgataa ttaatgattt taaacttttt ctagttacat
    15961 aagcaaatgc attttttatc attattcaac ctccaaattc attttattta cttttttcaa
    16021 ctttttacca cttagttcta aaatgatatc cgcagaatct gctacttcct tactatgtgt
    16081 tacaactatt acacatttat ttctatcttt agctaatgtc tttaatatat taattatttc
    16141 tccagcagta acactgtcta gattaccagt aggctcatca gctagtatta ttggagcatc
    16201 tgataccaat gctctagcaa tagctactct ttgttgctgt ccaccagata atttcataac
    16261 atttcttttt atttgttttt tatctaaacc tagttcaaac aagatacttt catctgctga
    16321 tttatttact aatctaatat tttcaattgg tgataaataa tctattaaat t
//
LOCUS       DACDCJ010000064        16150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDCJ010000064 DACDCJ010000000
VERSION     DACDCJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.064"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(51..548)
                     /locus_tag="IV038_002059"
     CDS             complement(51..548)
                     /locus_tag="IV038_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP8342258.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(678..1388)
                     /gene="budA"
                     /locus_tag="IV038_002060"
     CDS             complement(678..1388)
                     /gene="budA"
                     /locus_tag="IV038_002060"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP8342259.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1401..3065)
                     /gene="alsS"
                     /locus_tag="IV038_002061"
     CDS             complement(1401..3065)
                     /gene="alsS"
                     /locus_tag="IV038_002061"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP8342260.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3270..3932)
                     /locus_tag="IV038_002062"
     CDS             complement(3270..3932)
                     /locus_tag="IV038_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP8342261.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3942..4757)
                     /locus_tag="IV038_002063"
     CDS             complement(3942..4757)
                     /locus_tag="IV038_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8342262.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5019..5462)
                     /locus_tag="IV038_002064"
     CDS             complement(5019..5462)
                     /locus_tag="IV038_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP8342263.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5596..5934)
                     /locus_tag="IV038_002065"
     CDS             complement(5596..5934)
                     /locus_tag="IV038_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8342264.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5922..6299)
                     /locus_tag="IV038_002066"
     CDS             complement(5922..6299)
                     /locus_tag="IV038_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP8342265.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6523..7716
                     /locus_tag="IV038_002067"
     CDS             6523..7716
                     /locus_tag="IV038_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8342266.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7882..8304)
                     /locus_tag="IV038_002068"
     CDS             complement(7882..8304)
                     /locus_tag="IV038_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8342267.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8894..9349
                     /locus_tag="IV038_002069"
     CDS             8894..9349
                     /locus_tag="IV038_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342268.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9511..10683)
                     /locus_tag="IV038_002070"
     CDS             complement(9511..10683)
                     /locus_tag="IV038_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8342269.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10889..12318)
                     /locus_tag="IV038_002071"
                     /pseudo
     CDS             complement(10889..12318)
                     /locus_tag="IV038_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(12315..13817)
                     /locus_tag="IV038_002072"
                     /pseudo
     CDS             complement(12315..13817)
                     /locus_tag="IV038_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(13814..16141)
                     /locus_tag="IV038_002073"
     CDS             complement(13814..16141)
                     /locus_tag="IV038_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8342270.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4598 a         3232 c         2863 g         5457 t
ORIGIN      
        1 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
       61 ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga
      121 aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg
      181 attaaattac cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag
      241 gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct
      301 gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac
      361 ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc
      421 aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct
      481 tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct
      541 gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt
      601 tgtaaacgct aggtttcctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg
      661 catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca
      721 atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg
      781 cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta
      841 tgtgtatcat cgataaaatg caagtgaaat cccgctgcag caactccttg gaataattca
      901 ggcgtaaaga acccgactaa tgttccttgg acattgcttt gttggaattc tggttgagac
      961 tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc
     1021 atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt
     1081 actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta
     1141 tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca
     1201 actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca
     1261 agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct
     1321 gttccatcca tcaagccagc catcaaagct cctaatgtcc cgtgctggta taagattttt
     1381 tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc
     1441 tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct
     1501 tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa
     1561 gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat
     1621 ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg
     1681 atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca
     1741 tcgccggaaa ctgatacgat ttgtgtattt ggacgcacca atgcagcaga aatcgcccat
     1801 ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa
     1861 cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata
     1921 tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct
     1981 tctccttttg attgaacaat gtcccgctcc gttaattctg cttgaagaga tgctaaatat
     2041 tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg
     2101 gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc
     2161 gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat
     2221 tcgattggat catagccaat agcaataact agatcacttc gtttcaaaag catatctcct
     2281 ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc
     2341 actccagctg cttggaatgt ctctacaaca ggcaatgctg tctttccaac taattgcctg
     2401 atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt
     2461 gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta
     2521 ggatcgctca atggcttgat gacattccct tggacaggtg catccactac atcttgcggg
     2581 atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc
     2641 acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac
     2701 agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt
     2761 ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag
     2821 ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg
     2881 attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct
     2941 tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa
     3001 tcaacatgat gattaatcaa actttctact acagatttcg aaccttgttt cattttttcg
     3061 tccattgaac atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa
     3121 ataattgata cagtttctaa acctgtttca ttcaaaagta tagcacgttt tatttcattt
     3181 gtttagccat atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa aaactgtcat
     3241 tcttaattga aagtttcaat ttataaagtc tacagatcca tagttctcat caatttacga
     3301 ttgttgaagt aaatcaaacc aaatacgata gaaggaccta gtagaataca ccattggggc
     3361 tttacgaaaa agaagagaaa caaacttgtc aatagtaaaa aatctagtaa actcacatac
     3421 cattttttca acgtatagta gaacgctatc cgtagaactt gatttggttt cgacgctgga
     3481 tttctcacct gaaaatacca gtagttgatg ctaaaggaaa ctgataaaca agcaagtaaa
     3541 acaaacaacg gaatcaacca ttttccataa gaaaaacgta taaaaaagaa acaatcaaaa
     3601 atcattatca cactaactat ccaagcaaat agccaataaa aaaatccctt tagccaaaat
     3661 tggcgaaacg tctttatgaa caaacgtact ggtgaaaaac ttttttcttt tgctaattct
     3721 tctgtcatac ctatcatcac gatgcaacct ggaccaataa aaagtaaaga aaaaataaag
     3781 gaaaagacat tccttccatc tatcgacaaa aggaacgcag ctgctatata caagatattg
     3841 acacaccaaa aacaaaggct acttgccaat gattgataca accaataaaa gaattccata
     3901 taaacatttt tgtcaaaacg ctgctctttc ataatcacct cctattcctt cattcctgat
     3961 gaagcgatcc cttctacaaa atatttttgc atcgctaaaa atataaaagc aacaggaaga
     4021 acagaaacaa ctgaggcagc catgattaat gcgtactctg catcaaatag cccaataaac
     4081 attttcaacc ctaactggat tgttttattt tccgtagaag acaaataaat gaatggcccc
     4141 atataatcgt tccatgtatt aacgaatgtg atgatcgtca aactggcaat cgccggtttt
     4201 gtaagaggta aaatgatctt gcgatagatt ccccactctg ataagccatc gatccgcgca
     4261 ctctcacata aagaatctgg gatactactg tagtattgct tcaacaagaa cacaccaaaa
     4321 gcattgaacg cttgtaatag caccaacgac cacaacgtat atgttaaacc cacttttctc
     4381 atcataatga actgcgggat catataagac tgccaaggga cagcaatcgt gccgatatat
     4441 gctaaaaata aaatatcgcg ccctttaaaa tttagtttag aaaacccgta agcagcaaaa
     4501 ctagaagtaa acaactgaat caacgtgatc acaacgctta aaaaagctgt attcttaaaa
     4561 aatgtcacta aaggaatacg agaccaaata accagataat tctctaaatg ccaagtttta
     4621 gggatccatt gaatcggaat cgtgaacact tcattatttg ctttgaatga agcagaaacc
     4681 atccataaaa aaggaatgat cgtcaataaa gctaatacaa tcagcagaat catacccaac
     4741 cattgaataa agggcatgtt ttttgaacga atttttgctt tttttctttc atttcgaaca
     4801 cctccaaagt tataatcttc atttctttgt aatgattaca atccagaaca acggttatta
     4861 tagctgattc atttccgttc ttcttatgtt tgtaagcgct tatttaacaa gttatgtaaa
     4921 tctctttatt ttttttgatt taaaaatgaa aaaatacaaa agcaagactg tcatacgtct
     4981 cgcttttgta ttgaaatttc ttcgattaga gatacttatt actgttcaaa agcttctttg
     5041 aaacggtgaa tcaattcatt gactacaggt gataaaatcc gattcttttt ccagactagc
     5101 atgcagttcg tttcaatcat tgggtcaagc ggaacgaatg tcactcgctt tgaaagatga
     5161 cttacagcac cttctatgac taaagcagca cctacctgat tctcaattag cgcaaataca
     5221 ttgaaaatca aattgtacgt tcctgtgatc agcaactgct ccttttttat gcctaaccag
     5281 tcagcaaaca ttcgctgtat ttctggacgc tttgagatca aaagaggtac attcgcaaag
     5341 tcttcttttg ttatcacgtc tttttttgct aaaacagaat tttctaaaat gagcaatccc
     5401 catttttcct ttcttggtaa caaaatcgtt tcgtatttct ctgtatctac tggatctagc
     5461 aaaatcgcta aatctaacag gcttttttct agtcactccg tgatatcatc acttgttccg
     5521 ctgtggatat gaaattgaac gctgggataa tcacggatca acttttctag taatagtgcc
     5581 aatgtattcg aattatcagc ttccacacag ccgatcgtaa atcggccgct gaataattgt
     5641 ttttttctgt tctcgaattc ttgagtggtt tgattcgtta aatctaaaat ttcttctgct
     5701 cggctttttt agaaaataac ctgcttctgt caagatcatt ttgcggtttt cgcgtacaaa
     5761 cagttctgtt cctagttcct cttctaactc cttcagttgt cggcttaagg ttggttgtgt
     5821 aagatgtaga acttctgcgg cttgagagat tgttccttct tctgcaattg tccaaaaata
     5881 ttttagtaag cgtaactcca tagcgatgct ccttgatttt tttacttgat acattccatt
     5941 ttatttctag ataacacacg atcatatttc ttgatttttc cttgtaaacg ttccaaacat
     6001 tcttgcattt cagcaatttt tttcgtcaaa agttcacttt cttcaatcaa aagcttacgc
     6061 cgctcaaata cagttgcttc tccttcgcta gataggcgaa tatattcaat caaagactcg
     6121 atcgataatc cggcatttcg catacagatc gtaaagtcga cccattttag gtcttcttct
     6181 gtataatcac ggattccgcc ttttgtccga ttaactggtg gaatcaaccc gattcgttca
     6241 taatagcgca atgtatcggc cgaaatgccc ttttcttcac tgacttgttt gatattcatc
     6301 tgatcactct ttctcttaat ttcttgccac tttacttatg tattttattt attctacaac
     6361 tttaagtgta ctccaagtca atactcattt ctattaaaac taagctggat tatctatatc
     6421 ggttgtttgt gtattatttc aaataaaaaa gtcttgtttt tttattcttt tttggatata
     6481 cttttaacaa tttagggaaa ttttaggaaa aggggtataa aaatgacaac gtatcagcga
     6541 aagttattat tatcgacatc ggcgggaatt gctttagaga atatggatat tatgttttta
     6601 gctttttcat tatcttcaat gattacttct tttcatatta gtggtacgca agcgggtttc
     6661 atttcaacga tcactaattt ggggatgctc attggaggta ttttttttgg catcatagct
     6721 gataaatatg gacgtgtgaa agtattttct caaacggtcc ttctgttttc gattgcttct
     6781 ttattgatgt attttgcttc aaacatctac ttggtttatc tctttcgctt tatcgcaggt
     6841 gtaggagcgg gaggagaata cggggcgtgt atgtccttga tttccgaaag tttttcaaaa
     6901 aaacaaattg gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg tgcggctttg
     6961 gctgcgattt tagctgctgt gatcattcca acgtttggtt ggaagatgtt gtatgtcgta
     7021 ggtgtactgc cggtgttgat ggtcttgttc attcgtcgcg gcttgactga accaaaggaa
     7081 ttcaaatctg tacaatctgc cgaaaagaaa accaaactta gcagactgtt tgaatcaaaa
     7141 catcttgctt ggcaaacatg tggccttagt atcatggtaa ctgtccaaat tgccggctat
     7201 tttgggttaa tgaattggct gccttcaatt atgcaaaatc gattagggct ttcggtttct
     7261 gggtcttcat tgtggatggt cagtactatt ttaggcatgt ctttggggat gatgactttt
     7321 ggcataatca tggataaatt ggggcctaaa ctagcctttt ctttgttttt aatttgttca
     7381 tccttatctg tctatctact tgtgttagcc catagccagt ggagtttggt agcagccgct
     7441 gtgatagtcg gctactttat caatggcatg tatggtggat atggcgcagt gattagtagt
     7501 ttgtatccaa cagaaatccg cgcgacggcg aataatttca ttatgaacct tggacgagct
     7561 gttggtggtt tttcttctat cgtcatcggt ttcttgatgg atcattatag tttaactgcc
     7621 gtaatcttgt ttttgagcgg tatttaccta gtcagtctgc tggtcttgac aactcttact
     7681 ggtgttacag cattaaggga aagcttgcag cattgatagt tgagtctgta aatgacaaga
     7741 aagaggtgtg gcaaaagctt gcatctgcgg taataagacc agccaaatca aaaacatgag
     7801 acactgtttc cgtttggctg gtcgtattac ttgatgcagg gcagcttttt caactgctaa
     7861 tttccttcta tctggcggct actattcttt gacgtccgtc atttcaaagt actttactaa
     7921 taacttagca taacgaaaaa ctgtggatac actcgtatat tgagcgtctg cataatcttc
     7981 caaatttttc tcaggatgtt gatacatgaa ttcaatcaaa tgataattat atgattttct
     8041 gtagaaatga agataaataa tgtagattgg gaagttctcc tgcttttcca tagaaaagcg
     8101 ccgattattc tcattcttca aaatgacctt atcaggatat ttttggaaca attcttctaa
     8161 atctgttaga taattcaaga tagacttact tgataccttc aattcgtttt gtagttcttc
     8221 tattgaacac caacgttctg tttcaagcag taaatgaatc attttaattt tcttttgaga
     8281 aacttttgtt aaaaaagtct gcatattttc tcctcctgta tctactttac tatactacat
     8341 acaagaaaaa aaacggatac aaatatcata ttgtcacacc gtcaatagtg cataaacctg
     8401 ttattatttc tctttttttc ctaagtattc tattgatatg aatcatttaa tcgtttatta
     8461 tataaatgtc aataatctaa taataaaata acgtacatat tttattattg catacttaat
     8521 aacagaacta tagcaaaaaa tcccgtgtat caaaacggga ttagattaag tttcctcaat
     8581 cactattcat aacacaatta ttataacaag taagtaaaca attgaaaagg acatttattg
     8641 aaaacggttg ttttttactt aaggaggaaa tttagtgaaa aaattagttg cattgttttt
     8701 ggtcgcagga agtttgttga gtgggtgtgg tgctactacg tctactactc aaagcacaag
     8761 ttcagctcag gcttccagca gcagtaccac gatctccgaa agtacggcag attcatcgaa
     8821 agaacaagaa caaattgaaa agaaaaaaga agaagagaaa caaaaagcgg aaaaagctgc
     8881 actagaaaaa aaagtgacag aagcggaatc tgctttgtca caagctgaag caaatcctac
     8941 aaaagaaaac tatgcaactg cttcagcagc tatccaagcg ctacctgaaa acaaacaaga
     9001 gttgacaagc cgtcttgctg caattgattc cacaatcaaa gcaaaagaag cagaagaaga
     9061 acggaaaaag caagaagaaa ttgcacgaca acaagcagaa caagcgcaac aagaagcaat
     9121 cgcacagcaa caagcagaac aggcgcgaca gcaagcagca gctgaacagg cctctcagca
     9181 ggcacaagcc gcagctcctg agcaaaataa tgaacaaact gtctatgtca caccgaccgg
     9241 ttcaaaatac catacacata aatgtggaaa tggtacgtat acccctgcca ctttaagcga
     9301 agctcaagct cgtggtttaa ccccttgctc caaatgttat ggaaactaat tcaaaagtta
     9361 tactataata ataaaattct tctgaaacat aaaaaaatag aagctggcgt tccagatagt
     9421 aaaccaaaaa tagcagttta cacgtccatt tcgtcagctt ctctcttttt tcatttagtc
     9481 tgttccctct gcttttttgg acatcttcat tcattctttc tcaatcaaat agtacacctc
     9541 tttttctttt cgcagtttat atcttccttt tttcaatttc attgaaaact gtttcgaatg
     9601 tttatgtttt acatggattt taagcaggag ctcctcatgt tgcaagcgat agttcctgcc
     9661 ttttaaatca gaaaatctca ctagtccgta ttcatcagaa gctttcacaa atggaacttt
     9721 ttctctagta atatagtgtt tgccgttgag cgtttttaca gaaaagacca ttccttcgat
     9781 tggtcgtccg tttgtgttct ttacataaaa agataaagga tagtttcgct tagcgatggc
     9841 caaagaatct aaataagcga tgagtagcca aactaagccg attgatccaa gtagtatacc
     9901 gataaaccaa ataaaaaatt tccatttttt ccattgatca attccttgga tcgctttttt
     9961 tgcctctttt gcttgataag gaatacgatg ccccgtcaca agcagtcgat gtgtattgac
    10021 catataagga gtacaggtaa gcaaagtcac gagatctctg cctttttgga tacccaaaga
    10081 atctacttca tcaggtaaga ccacttggat tttttctact tgatacgcca atgtctttcc
    10141 attgatctgg atgaaaaact ggtctccttt tttcaactta ggcaaatctg taaataattt
    10201 tgcttctggg agtccacgat gcccagaaag aacggcatgg gtgtttttcc cgccagtcgg
    10261 ataagaactt tcttccaata atgacgttcc tttttgcaaa aatatttccg ttgtctgatc
    10321 gaatataggt aagctgacat tgatttttgg gatttcgatg actccaagca tatgttccat
    10381 aaaaaaggca ttactaggta aatcttttaa tgcttttgca tcaactgctt ggttgaaact
    10441 gcctatccct ggtgatacgt tttgctctgc caactgttgg tttttctttg tcattttttc
    10501 ctgctgtttt tttatttcct ctgaattctt attcgatgct tgcttttgat aatggatgat
    10561 catttgctgg gcaagaaaat cattcagtga atcttctaca aaaggataag aaaagatccc
    10621 gatcccaacg actaaaatga ttgccatgaa gatgtccagt attcttattc tgcttttatt
    10681 catcatgtcc acctattttc taatatgtat atgaagaggt tgtgaaatca gcgccttgac
    10741 ctgagtattc gctcaacaaa cggcaaaata gttcctccta ttttgccgtt tgttgagcga
    10801 ataccaagag accgaccact tgtcacaacc tttctcccca tttgaatcac tatcgtttat
    10861 ttatcgtatg atttttgttt ccaatagttt aagcttctgt ttcttgatgg cgttttttga
    10921 agtagatcac tgctcctcca actaaagcag tacctaccaa gatgaatacg atgatcccca
    10981 taccaccagt tgaaggcaag attcctttag gttggttatc gatcgttgtt tcatcagaag
    11041 ttgttggatc atcatctact gtaaattcag tgtacgcagc ttcattctta ggtaatgcgt
    11101 agccttctgg tgcttttgtt tcttgaaggc agtatgcccc ttgtttcagc ccttcgattg
    11161 aaaatttccc ttctgaatca gaaatcaatt tcacttcttc ccagtcagaa cctgtgactt
    11221 catctgacca agcatatggg tattctcctg aggcaagatt tgctattttt ttcattaatt
    11281 taacagttgg atcttttgct cttagaatag aaaattctgc atcggctaat ttttcttgcg
    11341 tgttcacatc ttgtttgatg aagtggaaat caccgtagtt ttggacattt tctacagtaa
    11401 tcgtgttatc cgtagttgta ttttcccgat tgactacacc gtttggattc ccaagattgg
    11461 cttcatctgc gccatggatg ataccggttg tttgttgcaa ttttccgttt tttactataa
    11521 aagtagtttc tttttgtgga ttggttaatt gataaccatc tggtgctttt gtttcaacta
    11581 aagtataatt gccgtctatt aatccattga cactgatgga tccttcagta tcagttgtga
    11641 atgtcaacgc tgactcagca ggatcataga gagcgacacc agtgttttca tcaaaaccgg
    11701 tcacgaattg attctctgaa tttttgatct ggaattgcgc ccctgctaac ttatctaccc
    11761 ctgaaaagtt cccagctttc tggattttga tattgctttc tttgatgaca tttttagcat
    11821 ataggtgaat cgtgtctaaa ggtttgtcag ggtctgatga attcgggtta taaaagggta
    11881 agcttaacac gatatttggc gacgtttcga caccgggctt tggtgtttct acaaagacgt
    11941 atacagcatc tttcccatca gatttacctg gaagagtcag gttagctaat ccttcaccag
    12001 aagtcgttac acttgcgaag ggtgtagcgt ctgctggaac tgctgtgtct ttacttggat
    12061 agtacgtagc agtgatgtca tatacgttga attcaacgcc gttcaacggt tcgctattcg
    12121 gaaatgtcaa ttccgttccg ttgtttattt gattttctgg tgctgaagtg aatttcaatt
    12181 tatgcaaatt aaccgtaaca ttttctggtg taggtgatac ggcctagaaa cctctgcagt
    12241 tcctaggccg agaacgaaag gaagcagcat gatagcagtt cctaaaaacg tccatatttt
    12301 tgttttgatg tttttcattt tatttccctc ctattgtttc ctttttgttg ttttttataa
    12361 aaccaagctg tccctgttga aaggactagc aacaacccta aaattgagaa acctccaagc
    12421 atctcttcgc ttgtttttgg aaagcttcct ccattttttt catttgatgg accatttgtc
    12481 tgcatctgaa ctggcgtttt tccattattc gtcggatttg cttttggttc gttgatgatt
    12541 tccaatggta taggatgaag cggatcataa ctgccttttt caacttcaaa gagaatggtt
    12601 gattgactca atatatatcc tttaggcggt ttaatctcca ctagttcgta acttccaaaa
    12661 gacaatccat ggacagaaaa tgttccatct ttactggaag taaaaatcgt tgctcttgaa
    12721 tcatctttgg ctacccaatg atttatcccg ttttcttgcg ctaagtattg gtcatttttt
    12781 ttgactagaa attgagcatc tgctagtttt tgtttcgttt gccgatccac tttgacaaat
    12841 tgtttgcctc ctgtttggat ggcaagatgt gtttctactg tttgattgtc agtcgtcaaa
    12901 cgtacatcgt tatcaaatgt cgttttttca ctgttttctt tcaatgtcat ttggtaacgg
    12961 atcgttaatt ttttcccagc aaacgaacta agatcttttc cagaatctga taagacaaaa
    13021 ccatgttttg ttttttgtgt gtgaaatgta tgaatcttgt ctccaccaat caaaaaatcg
    13081 atcgtttctg gttctagcca taactgagga tcagctgtat ctttgatcgt ataggttcct
    13141 atctcgttga tgttctcagg aattgttacg gtgatgagat aaggaacttt atccccatct
    13201 gcaaaatctg tctgcttatt ctctactgtt tttgttaatg tgagtgcagc aggtattttt
    13261 tgttcgctct ttggatacaa gtggatggtc gtcaatttat gattagatga atctgtcaca
    13321 ggaagcacaa cgacaaatgg tgctgatttt tcttttactt aatctgaagt ctttatttcc
    13381 gcaaatcggt aaacagcgtc acgaccttgc tcatctttgt ccgatgcact aaaagaagca
    13441 atgccctctt cctcgtctac tgtttttgtc acattttctg ccagtatttt ttcactatca
    13501 cttttttgtg ctagttttct ttgtgcgtct tccacactac ttccttctga ccgcagcttg
    13561 taaaattcat ccgagacatc atatatagcg aaagtaacgc cgttcaaccc ttttactcct
    13621 tgaaagttat ccgggatatt tattccgtcg ttttgactga tacttggcat cttatcgctt
    13681 tcgaacattc gattatgcaa agtaaatgta ataggtgatt ctctagctga cacagtcgga
    13741 gaacctataa aaaagaccag taggataaaa aagagaacag caaatatatc cacacgtaca
    13801 ttttttgttt ttttcatcgc tgttcacctg cttttttctt acgatggata aaataaaaga
    13861 agccagaaaa aataaagatc agtcctaaaa agacaaaggg cagtagacct atcccacctg
    13921 tcgaaggaag caagagagtc ggattgatcg tcaacttagc ggtatctgtt gcgataatcg
    13981 tataattatt gtttacactt ccttcgccta aactaatcgt tactatttgt gatccaatat
    14041 ctgtccggct gttaactgtt agggtataat ccaatgattc gcctaagaca acaccggtaa
    14101 cgtggagatt tccaaccgaa atcgagtgac tttgggcatc ataattaaag gcagcgtcat
    14161 cgacagtaat cactgcgtta gctttataga tggtataagt tcctgatgtg ttggctatat
    14221 cagtaaatga caacttggga tttgctgctt tgactttgtc tattccgcta ttcgttagtt
    14281 taattgtata gctgccagca ttaaccatat tttttgctga ttgtgcatct gctgtgtaat
    14341 ccatactgct aagtgccact tgtactggag taagcgaact agtgacaccc tctgctagct
    14401 tgacatggat tgacgggaca cctgcttttc catcatatgt cttactgcca ctgagctcta
    14461 ccgcaccgct gatttgctta atcgtataga catcagatgt ctgcacgata gtaaataaat
    14521 agtttgttct ggcagctagt ttagctagtc cgctttttgt caattgccaa gacgtatagg
    14581 ttccaatttc agtaggcgca gttacttcaa ctcccttttc attcatcgct ttgaaatcag
    14641 ttggattcca tgtgatttga tcaatactga gatcattgtt taaaagggat gttgattctg
    14701 ttttatcatt aaaggcatat ttaacgatcg gtacaacaga aagaccatca tagtacttac
    14761 tgccatttgc attgattgat actgttgcat ttgtcttata atataggatg acttcagaaa
    14821 tctgttttcc atttgcgtcc tttacgatta catttccatt tgtatctaaa ctagctaaaa
    14881 cactcattgc tgattggttc tttgtttctg tcgctgaaac agctgtaaag gtatatccgt
    14941 tgatagtagg cgtattagtc gtcaggtctt gtgcagcact tccgtaagta agattcatta
    15001 ttttagaagg agcaatcatc tgtccaaaat agtcttgata cttaatagtg attggtgttt
    15061 gtttgcctgt ataggtcacc gtatcgacgt tactttctag ggctgtgaaa gaaccgtttg
    15121 tttgtgcaaa agaatagccc gttactgctg ttggagtgat aaccactttg tcacccacat
    15181 ggattccaga taatgtagtc ccgtacgttc cgatttttgc tgctccacca ttgactgtat
    15241 aagaaaaagt tcctgtccga ttcgcattga cctgaaccgt tgctgcttga taggttggaa
    15301 aatttgaaac atttccctta ccatcaacga tctgcccatt tttgatcgag taccccgtga
    15361 atggaatatc atttgctaac tgtataccga ttgaaaaagt actcatacct cctccggcag
    15421 gaagccctcc ccatgttgag ggtgtaaaag aataactttt atccgcattg ggtgtaactg
    15481 aatcgatcgt atttccagcg gtagccaccc ccatatagat actttttggg aatgtgatag
    15541 tagtaccatt tactgtggag ccaataaaca gattatggat atctggacgg ttatcaataa
    15601 aagcgattgc tttggttacg ccttgtaacg cagttttatc cgcactcatc gatccgttaa
    15661 ccgcaacaat gactccatct ttggcatata tcactgtata tccatctggt atggtatgtc
    15721 cgttgaaatt cgcagtggcc gcaacagtac taaatgtctg gcttctacct gtttctgaat
    15781 aagctttctc ccctgctgct tttgctgctg tgatttcttt ttctgtcgga tcaatcagtt
    15841 gagtcgagga tgtggttgag gttgtctcgt tgttgctaga ctgattggtg ctttctatgg
    15901 actgtatttc agaatagggt ggactatttg tctcctctgt cgtttggtta gcaaaatttg
    15961 acgtttctgt ataaacgttt acataaatac ttgataagct ccctaagaaa gtaaaaatca
    16021 agatgatcga aatcataatg ggatgtaagt attttctttt agatacagct ttgagtctag
    16081 aaacaaaatg tttttgacat atacgttgga ttttattttg agttttctta atcattttca
    16141 tccctcctct
//
LOCUS       DACDCJ010000065        16075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDCJ010000065 DACDCJ010000000
VERSION     DACDCJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.065"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(140..1126)
                     /locus_tag="IV038_002074"
     CDS             complement(140..1126)
                     /locus_tag="IV038_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP8342271.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1149..1715)
                     /locus_tag="IV038_002075"
     CDS             complement(1149..1715)
                     /locus_tag="IV038_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP8342272.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(1729..2145)
                     /locus_tag="IV038_002076"
     CDS             complement(1729..2145)
                     /locus_tag="IV038_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8342273.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2602..4536)
                     /locus_tag="IV038_002077"
     CDS             complement(2602..4536)
                     /locus_tag="IV038_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8342274.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4838..5773
                     /locus_tag="IV038_002078"
     CDS             4838..5773
                     /locus_tag="IV038_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP8342275.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            5863..6966
                     /locus_tag="IV038_002079"
     CDS             5863..6966
                     /locus_tag="IV038_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8342276.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            6979..7932
                     /gene="menA"
                     /locus_tag="IV038_002080"
     CDS             6979..7932
                     /gene="menA"
                     /locus_tag="IV038_002080"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP8342277.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(8867..10213)
                     /gene="gor"
                     /locus_tag="IV038_002081"
     CDS             complement(8867..10213)
                     /gene="gor"
                     /locus_tag="IV038_002081"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP8342278.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10347..11588)
                     /locus_tag="IV038_002082"
     CDS             complement(10347..11588)
                     /locus_tag="IV038_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8342279.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11606..12529)
                     /locus_tag="IV038_002083"
     CDS             complement(11606..12529)
                     /locus_tag="IV038_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP8342280.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(12750..15242)
                     /locus_tag="IV038_002084"
     CDS             complement(12750..15242)
                     /locus_tag="IV038_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP8342281.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15249..15716)
                     /locus_tag="IV038_002085"
     CDS             complement(15249..15716)
                     /locus_tag="IV038_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP8342282.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(15852..15925)
                     /locus_tag="IV038_002086"
     tRNA            complement(15852..15925)
                     /locus_tag="IV038_002086"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15889..15891),aa:Arg,seq:acg)
     gene            complement(15983..16056)
                     /locus_tag="IV038_002087"
     tRNA            complement(15983..16056)
                     /locus_tag="IV038_002087"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16020..16022),aa:Pro,seq:tgg)
BASE COUNT         4703 a         3351 c         2865 g         5156 t
ORIGIN      
        1 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
       61 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      121 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      181 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      241 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      301 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      361 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      421 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
      481 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
      541 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
      601 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
      661 ccatctgccc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
      721 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
      781 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
      841 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
      901 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
      961 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1021 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1081 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1141 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1201 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1261 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1321 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1381 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1441 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     1501 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtcttg ttttttcatg
     1561 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     1621 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     1681 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     1741 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     1801 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     1861 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     1921 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     1981 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2041 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2101 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2161 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2221 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2281 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2341 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2401 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     2461 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     2521 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     2581 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     2641 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     2701 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     2761 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     2821 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     2881 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     2941 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3001 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3061 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3121 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3181 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3241 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3301 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3361 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3421 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     3481 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     3541 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     3601 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     3661 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     3721 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     3781 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     3841 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     3901 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     3961 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4021 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4081 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4141 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4201 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4261 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4321 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4381 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4441 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     4501 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     4561 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     4621 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     4681 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     4741 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     4801 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     4861 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     4921 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     4981 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5041 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5101 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5161 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5221 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5281 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5341 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5401 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     5461 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     5521 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     5581 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     5641 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     5701 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     5761 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     5821 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     5881 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     5941 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6001 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6061 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6121 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6181 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6241 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6301 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6361 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6421 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     6481 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     6541 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     6601 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     6661 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     6721 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     6781 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     6841 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     6901 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     6961 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7021 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7081 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7141 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7201 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7261 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7321 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7381 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7441 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     7501 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     7561 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     7621 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     7681 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     7741 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     7801 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     7861 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     7921 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     7981 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8041 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8101 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8161 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8221 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8281 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8341 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8401 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     8461 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     8521 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     8581 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     8641 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     8701 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     8761 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     8821 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     8881 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     8941 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9001 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9061 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9121 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9181 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9241 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9301 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9361 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9421 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
     9481 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
     9541 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
     9601 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacgaa
     9661 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
     9721 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
     9781 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
     9841 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
     9901 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
     9961 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10021 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10081 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10141 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10201 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10261 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10321 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10381 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10441 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    10501 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    10561 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    10621 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    10681 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    10741 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    10801 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    10861 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    10921 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    10981 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11041 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11101 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11161 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11221 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11281 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11341 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11401 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    11461 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    11521 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    11581 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    11641 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    11701 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    11761 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    11821 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    11881 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    11941 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12001 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12061 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12121 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12181 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12241 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12301 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12361 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12421 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagta
    12481 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    12541 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    12601 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    12661 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    12721 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    12781 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    12841 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    12901 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    12961 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13021 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13081 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13141 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13201 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13261 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13321 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13381 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13441 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    13501 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    13561 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    13621 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    13681 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    13741 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    13801 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    13861 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    13921 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    13981 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14041 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14101 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14161 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14221 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14281 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14341 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14401 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    14461 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    14521 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    14581 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    14641 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    14701 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    14761 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    14821 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    14881 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    14941 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15001 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15061 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15121 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15181 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15241 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15301 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15361 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15421 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    15481 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    15541 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    15601 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    15661 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    15721 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    15781 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    15841 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    15901 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    15961 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16021 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DACDCJ010000066        16075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDCJ010000066 DACDCJ010000000
VERSION     DACDCJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.066"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IV038_002088"
     CDS             226..894
                     /locus_tag="IV038_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP8342283.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IV038_002089"
     CDS             complement(1094..1480)
                     /locus_tag="IV038_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342284.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IV038_002090"
     CDS             complement(1452..2834)
                     /locus_tag="IV038_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP8342285.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IV038_002091"
     CDS             complement(2990..4177)
                     /locus_tag="IV038_002091"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP8342286.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IV038_002092"
     CDS             complement(4184..5215)
                     /locus_tag="IV038_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP8342287.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IV038_002093"
     CDS             complement(5231..6685)
                     /locus_tag="IV038_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8342288.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IV038_002094"
     CDS             6860..7765
                     /locus_tag="IV038_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP8342289.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IV038_002095"
     CDS             7856..8533
                     /locus_tag="IV038_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8342290.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IV038_002096"
     CDS             8814..9710
                     /locus_tag="IV038_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8342291.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IV038_002097"
     CDS             complement(9765..11135)
                     /locus_tag="IV038_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8342292.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IV038_002098"
     CDS             11373..12119
                     /locus_tag="IV038_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP8342293.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IV038_002099"
     CDS             12116..12955
                     /locus_tag="IV038_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP8342294.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYIFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IV038_002100"
     CDS             12956..13906
                     /locus_tag="IV038_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP8342295.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IV038_002101"
     CDS             14047..14799
                     /locus_tag="IV038_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342296.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IV038_002102"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IV038_002102"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP8342297.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5216 a         3003 c         3231 g         4625 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgctagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagt tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacgggt gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatatt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaa
//
LOCUS       DACDCJ010000067        15900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDCJ010000067 DACDCJ010000000
VERSION     DACDCJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.067"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(52..522)
                     /locus_tag="IV038_002103"
     CDS             complement(52..522)
                     /locus_tag="IV038_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP8342298.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="IV038_002104"
     CDS             complement(681..1139)
                     /locus_tag="IV038_002104"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP8342299.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="IV038_002105"
     CDS             complement(1310..2455)
                     /locus_tag="IV038_002105"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP8342300.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="IV038_002106"
     CDS             complement(2468..3385)
                     /locus_tag="IV038_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP8342301.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="IV038_002107"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="IV038_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP8342302.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYGTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="IV038_002108"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="IV038_002108"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP8342303.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="IV038_002109"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="IV038_002109"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP8342304.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="IV038_002110"
     CDS             6911..7792
                     /locus_tag="IV038_002110"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP8342305.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="IV038_002111"
     CDS             7789..8505
                     /locus_tag="IV038_002111"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP8342306.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="IV038_002112"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="IV038_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP8342307.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="IV038_002113"
     CDS             9222..10223
                     /locus_tag="IV038_002113"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP8342308.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="IV038_002114"
     CDS             10220..11581
                     /locus_tag="IV038_002114"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP8342309.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="IV038_002115"
     CDS             11541..12692
                     /locus_tag="IV038_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP8342310.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="IV038_002116"
     CDS             12667..13836
                     /locus_tag="IV038_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP8342311.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="IV038_002117"
     CDS             14011..15390
                     /locus_tag="IV038_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8342312.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="IV038_002118"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="IV038_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2802 c         3385 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcatctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acggcacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagatt atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DACDCJ010000068        15881 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDCJ010000068 DACDCJ010000000
VERSION     DACDCJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.068"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1029
                     /locus_tag="IV038_002119"
     CDS             <1..1029
                     /locus_tag="IV038_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP8342313.1"
                     /translation="NFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLLKARL
                     EIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMGFNKQ
                     LGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAESLGA
                     QEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFETIMTN
                     DSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDSYYVN
                     AEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSESSPS
                     TITKDWLE"
     gene            1870..2202
                     /locus_tag="IV038_002120"
     CDS             1870..2202
                     /locus_tag="IV038_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP8342314.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            2821..3162
                     /locus_tag="IV038_002121"
     CDS             2821..3162
                     /locus_tag="IV038_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP8342315.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3181..3471
                     /locus_tag="IV038_002122"
     CDS             3181..3471
                     /locus_tag="IV038_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342316.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            3663..3896
                     /locus_tag="IV038_002123"
     CDS             3663..3896
                     /locus_tag="IV038_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342317.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            4302..4973
                     /locus_tag="IV038_002124"
     CDS             4302..4973
                     /locus_tag="IV038_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP8342318.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPID
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLTLPIFK
                     GANQYTLSLGAATYFYEDAEMRKGNYVLAGHNMEIPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            5026..7005
                     /locus_tag="IV038_002125"
     CDS             5026..7005
                     /locus_tag="IV038_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8342319.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDVDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQAL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNNS
                     KGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELT
                     KFGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVE
                     VPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVID
                     KTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDT
                     AGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASL
                     HGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLK
                     VDSGLFGTGIKATPLESAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKS
                     DKEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKAS
                     VPMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            7020..7772
                     /locus_tag="IV038_002126"
     CDS             7020..7772
                     /locus_tag="IV038_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP8342320.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7788..8549
                     /locus_tag="IV038_002127"
     CDS             7788..8549
                     /locus_tag="IV038_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342321.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            8562..8822
                     /locus_tag="IV038_002128"
     CDS             8562..8822
                     /locus_tag="IV038_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342322.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8819..10909
                     /locus_tag="IV038_002129"
     CDS             8819..10909
                     /locus_tag="IV038_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP8342323.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            10936..11400
                     /locus_tag="IV038_002130"
     CDS             10936..11400
                     /locus_tag="IV038_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342324.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            11397..11639
                     /locus_tag="IV038_002131"
     CDS             11397..11639
                     /locus_tag="IV038_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342325.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            11657..11959
                     /locus_tag="IV038_002132"
     CDS             11657..11959
                     /locus_tag="IV038_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342326.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            12029..14092
                     /locus_tag="IV038_002133"
     CDS             12029..14092
                     /locus_tag="IV038_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342327.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHEGRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYSTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEKENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQIT
                     SEALMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEE
                     AQEQARKEYEQQLEELLNRTNQATTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            14092..>15881
                     /locus_tag="IV038_002134"
     CDS             14092..>15881
                     /locus_tag="IV038_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP8342328.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYRQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHF"
BASE COUNT         5952 a         2402 c         3173 g         4354 t
ORIGIN      
        1 aattttttta aggcggatag agtttacaac gaattatttt atcaatttcc aaaagtcttt
       61 attgtttctg acgaatacaa aaaaatgaaa gattcaacta agattgccta tatgctttta
      121 aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg atgaagaagg taatgtttat
      181 tttacttata cgacaaatga actatgtagc gtattaaact gccaaaaaca aaaagcgata
      241 gcaatcaaaa aagagttgga atcctttggt ttattattac aaaagcagat gggatttaac
      301 aaacagttag ggaaaaataa tcctaataga ctatatctag cagaattaaa agtctcagaa
      361 aatgatatct acttactcga aaaatttgat agagagaata gggaaaatgt tgataaatca
      421 gagggtatga aaatcatacc caccctcgac gaaaaatcag acgctgaatc ccttggggct
      481 caagagggta tgaaaatcat accgtgccaa aacgttgata aatcagaggg tatgaaaatc
      541 ataccagaac ttaataataa tatattagac actaatagac acaatataga cactgaaaaa
      601 gaccgcctac aagatcaatt gttgttagac aattttgaga caattatgac aaacgacagc
      661 attgctacgt ttgtccctga acgagtatta aatttgataa aaacattttc ttcaagttac
      721 agtgaagctc aaaaaaccgt ccagactatt cataatgcaa agaaaaaagc tgaaatagaa
      781 agtggtattt cgatagtttt tgaagaactc gatagttatt atgtcaatgc agaacaagaa
      841 ttatacacga cactgttaaa agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc
      901 cagaacctga tttttgtcta tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa
      961 gtatcaagtg aaaaacgttt gaattctgaa agctccccaa gcactattac gaaagactgg
     1021 ttagagtgaa actttactga caaagtgatt gttgcttcat ggagtaacat taaagttaaa
     1081 aattttttat tattctaatt atattacgaa tattacaaaa taatagctat tttaatagaa
     1141 tttataagtt aaaatattat gataaaaatc acaaacgtag ccttttacgt cataagtgtt
     1201 aaaagaaata attaatttca aatataaaaa agggaacgta tgtttgcata ctcctttttt
     1261 ttgagttata ataatacgcg aagagagata atttcattat ttaaacaaac taaaaatctt
     1321 gatattactt cttttcaggc aatacgaata ttattcgctt acattttttt gtataaattt
     1381 agttgtttta aatttaattg tctgctatgc ctgtgatgaa ttgattttcg ttctgcactt
     1441 ttggacgata aatttggaca attgaaaata caaaaaaaaa gaaaggcggt atgaactcgg
     1501 aaggctggta accttcgtca gcgttcatac acggcattaa gatttggccc ttaatgcgtt
     1561 aacctttcag tgttacggta ttacccgcaa tggatttagt ataacagata ttcaaaagtg
     1621 ataccacttt ttttggctgt gttaaggtta aaaatacgtg atattacgat gaatctatgc
     1681 ctaagcataa tgttataaaa atgtccgtgt ggctctttgc cgtatgacga acgctgaaat
     1741 tagagggaac gcatgtttgg atgcgttccc tcttttttgt ttgtcatgaa cgaaaatcaa
     1801 ttctctattt ttgaaaaata acaagaaaaa cttgttaaaa atatttaaag aaattgggtg
     1861 gtttatttta tgtcatatac aggaattttg agtctcaaag atatttgtca ctacggtaaa
     1921 cggtgtaccg ctacagagaa aatcacgaag aaactatcaa ctggccaaag taaaaccgtt
     1981 gtgcagtgta agaaatatat aatccagaaa gacaaagtct cagaggaaat gatttattac
     2041 attgggaaac aaaagcaaat aattttaaaa gatcctatcc ccttaaaaga gctatatcca
     2101 acaatcaaac atgtttatga ccaaaacggt gtactgattg gtcgaagaaa aaatggagtc
     2161 ttgcgttgta ccgcaaaagg gatggggcgt ttaatcagct gaattgatca ttctcataac
     2221 ttgtatttta tcgtttttcg cttgcttgaa aatattgtcg agacttctaa aagaacttag
     2281 aatcattaga aaaataagga gtagacttga tctatcaaca atttccttcg gggagtgacg
     2341 gggtagattt ttacgattgg taacgtaaaa aattggtcaa tcgagtaaat gcgcttgttt
     2401 ggatagtggt gaaatgaatt tgtgtaaata atatacacag agtaaacaag agggaattta
     2461 gaggttagac aagttagaat agcgcctaga ttaatcatga gcagctacta agggatctag
     2521 gctttgacgc aaactcatgg aataaggagt aggtcttctc gtaatgggga ggacgcacaa
     2581 gaaattgtgt cgtgtgctgc agtacatgta gatacttgcg aggacgttgg tcattgggtc
     2641 aatcgagcat ttaccgctgt ctactagggt ttctcgtttc ggacgccata aacgagtcga
     2701 tattttttat gtatttttaa gtaaacgaaa aacgataaaa tatgaacgag tcctgaataa
     2761 agtgaaaaaa ttgatataaa acatagagaa aatgaaattt acgtaatgga ggaatgacaa
     2821 atgaaaaatt atgatccgaa tatacgattg ggcacacata ctattaaggt ttctttccaa
     2881 cgttgggatt acaaaggttt tgtgacgttt agaagaggcg gaaattgtaa aggattagat
     2941 gttttagcat taaacgagga tgatttatac gatcaaacat tgactgataa tccaattgga
     3001 ttcggcttgc tacctgaaga tgatgaaggt aatgaatggt ttaaaatgac gcttatgaat
     3061 gataatggag acgaattatc tgttgaagat acttggtcat atttgagtga ttatattgtc
     3121 agcgttgaaa tcattgaatt tgtagcggat aaagaagaat aaaaactgga ggaataaatt
     3181 atgaaatttt tcgaagtaaa atcaccatac tattatgcgt taatcgcagc aaagaatgaa
     3241 gaagaatgtg cagaattgta tgaacaagta gtaacagata tcgaaaatcg tgaagaattt
     3301 attcgatgtt tgcatgagtt aaatcgaaat gaagcaattc aagaggatgc gaaaaaaatt
     3361 agtgaagaaa cgttacaacc aatcggtatg gaggaagcta caaaagacat ttttacaatt
     3421 attgatgaac aaaaatcttg tgtattaagc attgattcta cattggttta atagatccaa
     3481 gattctataa tttttttcaa agtaaaaaga aattaggtaa tgattctcag accaattact
     3541 taggaaaata accatacaaa ttagatttta cagataaagg attagaagga atcacggcga
     3601 ttaataaaaa ggtagtatta ccttaggcat tgagtaagga atttatttag gaggaaaatt
     3661 agatggcgcg accagtttta gatgcgatgg atatggatac tttgacaaaa ggaaggtatg
     3721 tacagctgtt agctcgtggt tattcaccta aagatgtatt caaagcccta tctaaaggaa
     3781 cagatatgga aaaagaaaga ttacgaaaag aattcgacta ttggagagag cataacggaa
     3841 taaaagattt aaaaccagca cgtccagtta ttagacgaaa aaaattgaag aagtaagacc
     3901 aaagcgtatg gcgtacatag gcttttttat tttgataaaa ataatgaaag gaaggaaaac
     3961 tcatgaattg taaagaaatc gaaaatagaa aaaaagtgtc gaaagaaatg gaagaaaagt
     4021 tactaaaaac aatgaaacaa aaacaatgaa acaaaaacac cttaaaagat tatctgtcgc
     4081 gcaatatatc aatgatatgc agttaacagg aaaagagaaa gcttgtttgt taggttcaat
     4141 gaaaaacttt gaacagttga gacgaactta tgtgaaaaaa agttctaatt gtcaactatt
     4201 gttagaagtc tcatgacatt cattaataga attcattttt aaaacttttt ctatggaaat
     4261 gagaaaaagt ttttttgttt tgtctgaaag gaggcagatg gatgaaaaaa tggttatttg
     4321 gttttttagg agtcgcttta atcgtctttt gcagtgtgtt tggctatgtt agctaccaaa
     4381 aacatgaagg agaggtgttt aagcaaaata tagaaaaaaa gatgcccata gatcaaatca
     4441 atgaacatgc aaagagttat aaggaggatg caacgaacgt taataatgat atgtctttgg
     4501 gacaaatgtt gtccatacaa aaagaagcaa tcgaaatggg cgtcaataaa caagcctttg
     4561 cacaaattca gattcctgcg cttggtttaa cattgccgat ttttaaaggt gcaaatcaat
     4621 acacattgag tcttggtgcg gctacttatt tttatgagga tgccgaaatg cgcaaaggaa
     4681 actatgttct agcaggtcat aacatggaaa ttccaggtgt tttattttct gatattcaaa
     4741 agttgtcact aggggaggtg atggacttag ttagtaacga tggcgtctat cgttacaagg
     4801 taacgcgtaa atttatcgta cctgagtact ttaaattaat cgatggcgtt cctgaagaga
     4861 actctttctt gtctttacca aagaaaggag agaaaccctt attaacgttg tttacttgtg
     4921 tttatacgtc acaaggaaaa gaaagatacg tggtacaggg agagctacaa taacaaggtt
     4981 ttaatcaacg tgagtcgttg aaatatgaaa cgaggagagt aaaaaatggc aataaatcgg
     5041 tatgtaaaaa aagctggaat gcttgttgga atgttaggaa tcattgcagg ctgctttcag
     5101 ggatatcgag catatgcaga agatgtcacg caaaaaacgc caccagagaa ggtcaatatc
     5161 acggttcata aattaatgta tgatcaagga acgcaattga atgttgatgt tgacgggatc
     5221 aaaaacgatg gttatacgca cgatcaatat cctacgggtg taacgaaata caacaaggca
     5281 gattatggtg atgttgagtt tacgttagga aatattacgg atcaagcatt accgagggaa
     5341 gattctgatc taaccaacgc gaaagtggac gaaatcgtta aagatgtaga agacaaggga
     5401 tctaacagtg agtacgtcaa gaatgcaacc aacaaaatta cttcagctgt agatgaaaat
     5461 ggtgagatta cttttgctga tcaaccagcc tatgtcaaca attccaaagg gaatgtatat
     5521 gtggtttatg aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa accaatggtt
     5581 gtgattgcgc caatgactga taatacaggt tcaggttttt taaaagacat tcacttatat
     5641 cctaaaaata ttgtttctaa gttgagtttt gaattaacga aatttggtga cgatggcaca
     5701 gctcaaagca aacaaacacc tttaaaaggg gcaaaatttg agttatacaa gggcgaacct
     5761 ggaaaaggaa caaaattagg agatcttgta tcggatgatc aaggaaagct gactgctact
     5821 gatttgacgt taggaaaata ctattttgtg gaagttcctt cagaagttgt agtgggatca
     5881 gataaagaac caacagccga tcaatattta ttaggcgcag acgctcgcaa cgatgcgcac
     5941 aataaattaa cctttgaaat tacgaatgat ggcgtgacaa gtgacttaaa agcttcatac
     6001 gtaaactata aagcaccagt gatcgataaa acagtaacaa atggaacagg acaagaacat
     6061 tctttccaaa ttggtgacgc agtaaattat caaggaacga ttcatatccc aacagacatt
     6121 gcaggcggag cagacggtat cacagtaaac ggagtgaaaa gcgaaacttc tccttacagt
     6181 gtatttaaat ggggggatac agcaggacaa ggactttctt atgtcgctgc taaagcaaat
     6241 atcaaagtaa caaataaaga tggatctgtg gtattaaaag aaaatacaga ttataaaatc
     6301 caaaattcag aaaatggctt tgtgatcgat tttatcgtga ataatggcca agtaagtgat
     6361 actgtagcaa gtttacacgg acaagatctt caaatgactt ataacatgta tgtaaatgac
     6421 agcgcagctg ttgccaatcc attaacaaat agcgtggatt tcgtttataa caacaatcca
     6481 tttaatcagg aagaacacca cgaaaaaacg aaagctgacg ttgtgactta tggtgcaaaa
     6541 ttcttaaaag tcgatagcgg actatttgga acaggaatca aagcaacacc gcttgaaagc
     6601 gcagaatttg ctgctaaaaa cgcagaagga aaatattatg gtggattagt tgatacggat
     6661 aaaaacggtg tcaaagaagc agtatgggta gacgatgtag caaatgcagc gatcctaaaa
     6721 tcagataaag aaggtcactt tgaaatcact ggtttaactg aaggtgaata ttcactggaa
     6781 gaaacaaaag cgcctgaaaa ctatcaaaaa ttaacgaaag agatctcttt caaagtagac
     6841 aaagattcat acaaagaaga aaaccgaatt acgatcaaaa acaatcaaaa agcttccgta
     6901 ccaatgaccg gtagcaacgg ttttcaaacg tatgtattaa tctcttgctt gttgctagga
     6961 gcaggggcac tttctgcagt tgtttacttt aagaagaaag cctagaagga tcaaaaccaa
     7021 tgattatcag gaagcctaag aaaaagcgaa tcatgggtaa atggatcatt gctttctggt
     7081 tactttcagc tgtaggtgtc ttacttttga tgcctgctga agcgagcgtg gcgaaatacc
     7141 aacaaaatca gcagattgcc gcaatcgatc gaacaggaac ggcagcagaa acggattctt
     7201 ctttggatgt tgctaagatt gagttaggcg atcctgttgg tattcttaca attccaagta
     7261 tttctttaaa actaccaatt tatgacggaa cgtccgacaa aattttagaa aatggtgtag
     7321 gtattacgga aggaactggt gatattacag gaggaaacgg taaaaatcct ttaatcgcag
     7381 gtcatagtgg actttataaa gataatcttt ttgatgattt accttcagta aaaaagggag
     7441 aaaagtttta tatcaaagtt gatggtgagc agcacgctta tcaaatcgat cggattgagg
     7501 aagtgcaaaa agacgaacta caaagaaact ttgtcactta cttagaacca aatcccaatg
     7561 aagatcgagt aacgctaatg acatgtacgc ctaaggggat caacacacac cgttttctag
     7621 tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa agatgaagaa aataagaaac
     7681 aaaaattatc ttggacatgg cttttaggaa gtactgtttt tctttcagtc atgatcattg
     7741 gttcgctatt tgtttataaa aagaaaaaat aaaggagggt aaccaacatg agaaatagaa
     7801 aatggctatt tgtttgtgca gccgctcttt tttctttcac tgcctacaca cagttgacgt
     7861 ttgcagacag acaagaaagc acggatagca cagaacaaac aatccaaacg tcagatagca
     7921 gcacgattca aagcagcgat gaaaccgtcg attcgtcaac agttgattct tcagaagaac
     7981 cgaaaattgc tgaagacgat caaatagtca aacagaccat tcacgttcaa aaaattattt
     8041 cttccgaaca attagacaac caggggcaaa aacttgaaaa acaagaagga gtaaatggcg
     8101 cgaagttttc tgtttatgat gtatcggaca ttttacaaaa gatggatgtt aaggatctca
     8161 ctactgacca aattgaatcc caattaaaag atcgagtgaa aaaactattc agtgatcaat
     8221 tgaagctagt ttctaatgga aaaacaaaga caattgatca acaaacaggt gtctttgaat
     8281 tctcgataga agtacaggca aatcagaaac aagcttatta tattgtcaat gaatcaagtc
     8341 ctgaaaacat ttctaatagc gaggatattt tgctattaac acctgtttct gataaaaacg
     8401 gtgaattctt aaaggatgtg tggatctatc ctaaatcaga agcttcgcaa cctaaagaag
     8461 aagtaaaaaa aatcgtttct actggcgtaa agaaaaactt ctttgagaat tgttgggatt
     8521 ttgttactca cttgttttct agagattaag gaagaaataa aatgaaactg atcaaattat
     8581 tattattcaa tttttcttgt ttaaccgcag tctttttgta ctgcggttta ttattttcta
     8641 aactatctat tttgccaagc taccaactat acctaatgag gattttaaac ataccagctg
     8701 cttccatgcc gctagatatt atggaaaagc tcattaggaa tccattattt actacgatca
     8761 atttgatcgt tattgtcgga atttcactag tcatttataa aaaagagggg atgaagggat
     8821 gaattttaaa cactcgtgga caagacggtt aaaatatgtt tttcccataa tgatgatgtt
     8881 aggtacattg tttgggttaa aaacagccaa tgtctttgct gaaaaagtta ttgtcgatcc
     8941 gtcaaatcca atccagaacg taaagaataa ttttattatt ccaaaagatg ttcctaatgc
     9001 aaaaacaaat attactgtaa acggagcgtc aaaagtttac aaatatcatg ttgatgccaa
     9061 caaaggtggt tttacagacg actacgtagg attagtggag ggaaacaaaa atatacgcta
     9121 tccttctttc caaaaagaat acggtgaaac aaaccttgtt ttagaaggcg taacaaccaa
     9181 caccaaagtc aatattgact atggaaaaat cggtacgtat aacgggaaaa aagtcaatat
     9241 caagctagtt ctatctaata ttcacttata ctcggatacg ttgccgtgga atctactaga
     9301 taacaactat acaaaaactc attttcgaga cgatggctat aaaaatacga atggtgcaat
     9361 gagtaaatct aaaaaaagaa cagtactttg gatctcagac aatcttttct caggaattgt
     9421 ttaccattca acacaaatga atgtgcagct agtagccaca tatgaggacg gtagccccgt
     9481 tcaattttca ggagatacat ttatcagttt taactctttg aaccctgcag gaggaaaaac
     9541 cacggatctt aaaggagaat atgcccacta tgacaaaatg aatacgacag attggtatgt
     9601 cagaaaagat acggttcttt cagaatttaa atctttctac aacaatctga acgtggtagg
     9661 tggccaccca ggaggaagct ctaagctgac acaagcggat aatgatttca ataatttgca
     9721 tgataaatta ggagatccga agtttggtca aggtactgtg tcctttaaaa tatctgaagc
     9781 aaatccgacg tttgtgattg gttcttctaa tgttcaaacc tggtttactt tatcttctgc
     9841 gacaatcttt agtgttgttc ctgatcaacc tgaaaaaaca ggggttgata aaaatggaaa
     9901 taacgtgaac gataaaatgt tgcaagtagg agacacgatt caatatcgaa tcaaacagaa
     9961 ggtgaatcga ttaggagtgg atttactagc ggtttacgat cgatttgagt taatcgataa
    10021 tttaccaaaa gaagtaaatt acgtcgatgc acacgttgaa agtggtacaa ataaaaaatt
    10081 cgatgtatct ggtgaagtga cctatgacaa aacaaaacac caggtaaaat atgcagcgaa
    10141 agctgataca ttaaaaaatc gaatgaagta taacggcgaa acttacgaat tagtgatcaa
    10201 tgtaaaagtc aatgaattgg ccaatcaaaa tagtgtagcc aaaaatcagg gaacgtcgat
    10261 cattaataaa gtagaaaagg atacaaatat cattaccgtt tatttcccta aaattccagt
    10321 gaaagaagta caacaaaatg gaaaagatgt aaacggacga aatgacggta agaagggtac
    10381 gccgacagct cctttaaacg caggatctga agtacaatat ctagtcacac aaaaatggca
    10441 cacaaaaggc gtagacgctg cgtcagatca ctacaaacaa tttagtattc aagatccaat
    10501 tgaagctcgt ttgacatata aagaaggttc tgcgcaagta atagataaga gtaaaggaaa
    10561 agatattact tctgaaggca cactaaccta tgatagcaat tcaagaacgc tgaagtggga
    10621 agcttccgct gacttcttaa gtaacaatct tttagatgga cgagaaattc agctgatatt
    10681 tacagctaaa actccattac aatcagaaaa aaatattgat aaccaagccg tagtagcagt
    10741 agataatgtt tctaataaaa caaatgttgt gacaattggg gttgatccta acttgccaca
    10801 ggtcattgtt cctaaaacag gttctacaca tttagtaacg atttcagcgg tcagcttact
    10861 attacttgtg ttagcaactt ttagttatgc ggttctgaga tacatttaaa ataaaaaaac
    10921 gaaagaatga ataaaatgaa aaagttaata atagtagtta cttccttgat tgccatgatt
    10981 gttattttag tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga taatggcgta
    11041 ttgaaatcag gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa tttgaaaatt
    11101 atagacgaga ctacctggga atactctgag agtgtatggc atccagatcc tgtacaaata
    11161 acagtcaaga ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt agattcagct
    11221 ggtgttaggg aatttataaa caaatctgat tctttattta ttgttgttcc ctatgagaag
    11281 aatggcgtaa aaaaaataat ttttcttgga tctagcaagg atgaaaagcg aacgaaagag
    11341 aaattgattc acgatggatc acaatccaat aaatataagt tacaaaagac tagcgagtga
    11401 ttgctagtct ttttatttgt cctgaaggag gaatcacagt ggcaaaagct aaaacgccat
    11461 ttgaaaaagc cgcgtctaaa atcttagaac gccgcggtgt atctgaaaca cagtggaaac
    11521 aagaagtcct tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag
    11581 gatatatcta tcaacgtgaa cgtgaaaagc ttgttttcga cgaaattatt aaacactaaa
    11641 aggagcgaac aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt
    11701 agtagctgcg gttgatattt ttagagcatt acaaactgcc gcaactagtt taaaaaacaa
    11761 tctgatgatt tttgctgctg ctgcggccgt tgcttccatt gttattgggg gtcttttctt
    11821 cctatttgga cgaagaggag ctgaaaatgg gaagatgcac gtaggctatg tattgatggg
    11881 ggtaggagtt attttagcag cagcttctat tgtggtttcg ttatttggat tgtttggaca
    11941 aacacctcaa ggattataag aaagtaaaaa agtaggagta agaagtagtc gatacttttt
    12001 actccttttt tatgaaagga gaatactcat gtactttacg attgcaggca ataccaaaac
    12061 agataaagag ttaataaaaa aagcaacgaa ggaagtagga aagcgtactt cttttttacc
    12121 gaaaacaatt aaatattctg tcattgaaac ggaagaacag ctacatgtgt tagggaaagt
    12181 actggaacac gaaggtcgat cttctgttaa gttgactctc acgttacatg acacagaagg
    12241 aaaaatagag aaagctggta cagaatacgt ttttggccca ctggattatc aaatttcaga
    12301 aggtttctat tctacattga tggatttaat ccgccttgat gtgtttaaag aaacggatga
    12361 aactatgagt gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt tagaagaaga
    12421 gttagagagc gaccaaagaa aagtgaatca agggagtgaa gtcgaagtaa atgaagaagt
    12481 accatgggaa cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga cagtctatgt
    12541 tgacgaacaa gctgatattc cgactcagaa atcgatattt gagaaagaga acgatgtgat
    12601 gggtcagttg gaggtagatc cctttgaaga aacaatggag gatgaagaga cggccgaagt
    12661 agaagttgat ccagttgaag ctaatgaaga gtcaacatgg actgaagggg attctcagga
    12721 atatattgaa ccacttatca gtgaagaagt agaaaaaaac gaaatagaga tacaaaaggc
    12781 aactgtattt gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa
    12841 aaacaaatta tttttaaatg agctttattc gaaatatcaa ttaccccaaa cctatcaagc
    12901 atttttagag agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt
    12961 agagtctgtc caatcaacaa attggcatga gcttgcacaa gaagatttat tagccacctt
    13021 tgaagaacgt caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc
    13081 taattgggag aaagcaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga
    13141 agtacaaaaa attaaagagc gatacgaaaa gaaacgcgac aatgcgtatt ccttgtgtgt
    13201 agaaaagatc aataatttta ctcagacgaa tcaagaagcg ttatcgaaag aaaaagaaaa
    13261 cttgttaaaa gaacgtataa gagaacgaaa agaagaattt tatcaaggtt tgcgtacaga
    13321 taagctaaag attagcaaag aattaaatga gcaactgaat aagctctatc aaataacaag
    13381 tgaagctctt atggaaaaac acgagcagat ccagcaagaa ttacaagtac aaattccaaa
    13441 atggaaaaaa gagtatgaaa ttcaacaaca agaattgaaa gaacaagaag aaaaacaacg
    13501 gcaaaaagaa aaagagcgcg aacaattaga actaaaaaaa cgtgaacagg atcttgcgga
    13561 acaacaaatg gcaatagaaa aacaacggtt aaaccaagaa aaggaaaaag aagcagctgc
    13621 tcgtaaggaa cgtcaagaac aaatggatac attacgacaa gctcagttaa attatctagc
    13681 acaacctcaa ccaacgcctt cagtagctgc tgttcaaacc ccaatgaaca accaaacaaa
    13741 tctaactcct tcaccacagc gttcctttaa cggatggatg gtgggatgta ttgcaagttt
    13801 aagcttgctt ctaggtggag ggacagtatt tgcttttaat catatcaatc aatcttcaca
    13861 ggctgattct gtagccacaa cggcgtcaat caaggaagaa gcccaagaac aagcaagaaa
    13921 agagtatgaa caacaattgg aagaattatt gaatcgtacc aatcaggcaa caacagagtc
    13981 aagtatggaa aatagcaaag agagtgatcc tagtaaggct aaagaaggat caagcgattc
    14041 aactgtacaa tcaaacactt ccgaaagttc agaatcaaat gaggtgaaat aatgcgtgtt
    14101 agagaaacaa cgagacgaaa agacaaacga ttcaatttag aaaagaatag gtatctttct
    14161 catttctatc gctatactcc ttcaggagaa gagctaccag aaaaacatga caaacgtatt
    14221 tctcaaaaga ttttttatag tggcttagct tatcttcctt taaactatgt tgttacaaga
    14281 ataccagaag catttcatcg atttgagatt attccaggaa gtttacaagg agtgaatatt
    14341 tttcagttac aagaaatgcc gcagttattc cattttgatt caactggttt ttggaatcct
    14401 ttgaccccaa caacaagttt aggcacacgt ttagttgttt ctacgatttg tgttgttcca
    14461 gctatactta tgggattgaa aatggacatg tggtttcgtc ctctagcaga tgggcaaaaa
    14521 ggagataacc gattaaaaac gttaaaggaa atccaacaaa tttatccgca aataccagaa
    14581 tcaaagggag gttttcctgg ttatggcgga ataccaatta ctcattataa aaagtactgg
    14641 tatattcaga cggatacagt aaatacttgt attgttggta cttcccgttc agggaaagga
    14701 cagatagaag ttttagcaac cattgacaat ttgtctcgtg cagaaagaca aagttcaatg
    14761 gttgttaatg atccaaaaac agaattatat gttgctagta aagatgaact agaagctaga
    14821 gggtatgatg tgtatgcatt taatatcacc gatccactgc aatcaatgag tgataatcca
    14881 ttggcactca tcgtgaaata ctggaagcga ggagacatag atactgcaac gcagttaacc
    14941 aatactttta cgaatacgat ttattatgat gccaatgctt cagacaacaa atactttaac
    15001 gaaaatgcgc agaaagctgt aaatgcgctt atttttgcgt tgttagaaca agccgatcaa
    15061 acaggggatt actccaaatt aacacccaat aatcttgttg agcttctttc tgaaattggc
    15121 ggttttaatt atcaagatcc tgagaatgat tacagacaac tcaatgcctt agatgaattc
    15181 atgaatcagt taccacctgg aaacatagca aaaaaacaat atggggcaac aaaaattggt
    15241 tctgataaag caaaagggaa tattttatcg acggcaatca caggattgaa tccatttacc
    15301 ctaacgaaaa ttgcgaaaat gacttctcaa tcaaccattg attataagtc cattggtttt
    15361 ccgaagtatt tggatctcaa actacacgag agtctgttaa atcagcgaat tgttgtagag
    15421 ttttatcatc aagggaaaaa atttcatgag gaacgtgtca aagtaggtta tagaggtttt
    15481 tgtgaaatca actttgattg tcatttagag cagggagatc atgtttgctt aaaatacgat
    15541 catcaaggaa aagaatatgt tgcttggtat gagttttctc aaagagtatt gaaagacgaa
    15601 gagggaaata tcgtttatcg aaaaaaacga ggagaaaccc atttaccaga attagaaaaa
    15661 gaagctgttc taacattaga tgaggataaa agtaacttat tagtagagtc tattcggatg
    15721 cactataccg ataaaccaat ggctattttt atgctcacac ctgattatga tccatctaac
    15781 catatcattg ttagtatttt tcttagtcag ttgtataaag aattatctac gcaatgtgtt
    15841 aagactaaag gagataaatg tcaccgtagg attcatttta t
//
LOCUS       DACDCJ010000069        15402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDCJ010000069 DACDCJ010000000
VERSION     DACDCJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.069"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(32..364)
                     /locus_tag="IV038_002135"
     CDS             complement(32..364)
                     /locus_tag="IV038_002135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342329.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(378..608)
                     /locus_tag="IV038_002136"
     CDS             complement(378..608)
                     /locus_tag="IV038_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342330.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(830..1372)
                     /locus_tag="IV038_002137"
     CDS             complement(830..1372)
                     /locus_tag="IV038_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342331.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1393..2484)
                     /locus_tag="IV038_002138"
     CDS             complement(1393..2484)
                     /locus_tag="IV038_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP8342332.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2492..4702)
                     /locus_tag="IV038_002139"
     CDS             complement(2492..4702)
                     /locus_tag="IV038_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342333.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4718..7246)
                     /locus_tag="IV038_002140"
     CDS             complement(4718..7246)
                     /locus_tag="IV038_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8342334.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7298..7708)
                     /locus_tag="IV038_002141"
     CDS             complement(7298..7708)
                     /locus_tag="IV038_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342335.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7721..7939)
                     /locus_tag="IV038_002142"
     CDS             complement(7721..7939)
                     /locus_tag="IV038_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342336.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7936..8928)
                     /locus_tag="IV038_002143"
     CDS             complement(7936..8928)
                     /locus_tag="IV038_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8342337.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9007..9918)
                     /locus_tag="IV038_002144"
     CDS             complement(9007..9918)
                     /locus_tag="IV038_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8342338.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9937..10281)
                     /locus_tag="IV038_002145"
     CDS             complement(9937..10281)
                     /locus_tag="IV038_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342339.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10301..10857)
                     /locus_tag="IV038_002146"
                     /pseudo
     CDS             complement(10301..10857)
                     /locus_tag="IV038_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10938..11438)
                     /locus_tag="IV038_002147"
     CDS             complement(10938..11438)
                     /locus_tag="IV038_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342340.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11439..11984)
                     /locus_tag="IV038_002148"
     CDS             complement(11439..11984)
                     /locus_tag="IV038_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342341.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11981..13237)
                     /locus_tag="IV038_002149"
     CDS             complement(11981..13237)
                     /locus_tag="IV038_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8342342.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13598..13909)
                     /locus_tag="IV038_002150"
     CDS             complement(13598..13909)
                     /locus_tag="IV038_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342343.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13932..15242)
                     /locus_tag="IV038_002151"
     CDS             complement(13932..15242)
                     /locus_tag="IV038_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP8342344.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
BASE COUNT         4655 a         3330 c         2234 g         5183 t
ORIGIN      
        1 taaaaaataa gttacttttt aaatatcttg tttatgaatt cattccccaa aggcctttta
       61 agattgatac agcagtccgt tcacttcttt tagacttgtc atacaagtct tttaaatgat
      121 cccatatcag actagtttgt ctatctagct tcaatccatt aaacgctaat gaacggttca
      181 ttttttctat tactttagat aaagctacag aaatttttat accttcacaa cgcgcatctt
      241 ggatttcaat aatagtatca tcaattagtt tttgaatttc ttgcctatca acatattcaa
      301 tcttagaaag agaatctttt aaagctacta atgattcaac gatttctttt ttctcttcag
      361 acattttctt cctccttcta gtgacccgta aagccatgta gagcagagtc cattgaacca
      421 gtaattacac tatctacaac gccattccaa tttactctac attttccatt tttcagattt
      481 gtcggtccat acggatagcc tttttgacat tcaatgttcg gattatacca gataggatta
      541 taccattcag ccgcagaagc agattcagtc gatacaaaca atcccacacc aacaaatcca
      601 atacacatca cactatagcc aattagtttt ttcatctcgt atccatctcc taaaaaatag
      661 aatagttctt atagttattc ataagacata ttttttccgt ttttgtttta tcctttctgt
      721 cctataacaa cttttttcaa gtaaagaatg acatagtttt tcttaaaaaa acagtctgtt
      781 tttttctatt tcttcggtgt ataataaata tgagtgttca ttttttattt taatacgcac
      841 tccgtacata ataaatcttg atcgcatcaa tcttttgagc ttcttgatct actttcaatt
      901 gaagtaatac aaattgagta accttattca ttgtttcttc tccaaataac aaatattgat
      961 ccggattctc tatatcttgt gtcacctggt aaagtttccc agttgttttc atttgtacat
     1021 gcgggtctac atcaatacta ttgtctttaa tacaacgatc tgtcatatag tccctcacag
     1081 cttgattgcg tttataaaca tctgtgaaat tactccattt tctagcaaaa tcttttaaga
     1141 aattttttgt catttcatct atttgcattt gttgcgctgt ctcgtcttct aatgaaccaa
     1201 tttgactgtt actttcagta ctttgaagag ttccacttgt ttcttttact gcttcgattg
     1261 tttcttctgt cgttctcaat tgtgtttttt gtttttcaga agaaaaccaa tgacaaccag
     1321 taagacctat caaacataac caactaaata ctaaaattat cccttttttc acgtttctag
     1381 tcactgcctt tcttatttca ccttacgaac cagactacga atttttgttt gattgtacgt
     1441 gcgttgatac cgagccataa tccgtccttg ctcggcattc tgttccacac tagagaaatg
     1501 ttgtccgcca tatgaaacat ctgttataat tccggtgtga ccatagattc caggagacaa
     1561 tgcaccacct gcttgccaat tcaccacatc tcctgcttgt aaatcacttg gttttgggtt
     1621 catgattact tgccaaccat aggttgtcca gttataatct tcccctatta acatcgcaaa
     1681 catgtgtcca gaacccatta actgtgggcc acctaattga gatacatacc atgccgtacc
     1741 accataacat tccccattat ttacggatcg tccaatgatc cgatccaaaa ccttcgcatt
     1801 gcctttactt tttcctgcaa taagcgaagt tttttttgtt ggtaggctat tccaatgctt
     1861 gatcgcaaaa gtatcatatt ccgttaagtt ataggacgtt atgatactca ttgctcctcg
     1921 ataatacgga ctcccttcgg taatacctgc tgccgcatag cctccacgac ctagaatcaa
     1981 taaacaactt ccaggatctt tattgcccac acattgatta aagttgcttt ggctcgccac
     2041 cataaagcca taatagtcat aacaagcttt taagttcgga aacttcatat agaaaccacc
     2101 ttcagaacca ccaattccac gtgcagaacc ttgtgggtag ggacaaccag gaaaccaggt
     2161 aatcccaaaa aagttcgtat cgtttcttcc agattgtgac tgtccataag ctgattcata
     2221 gcctaattga gaaagaatca cactcggtaa taactgatac tttacagctg caccaactgc
     2281 tgcttgcgca tgttcatctg tgattggacc acgtccttta atctctgaaa aaatcggtaa
     2341 tccttcggta tattccccat taaaagcgac agctagacct gtagcttctc ctccatgctc
     2401 agaaagttct cctgaaaaaa agaatggcaa tagaaaaaag ggaagaataa agaaaagaag
     2461 acaacctaga caccctttct tcatttcttc ctcatccttt cttcgataat ggtttttgga
     2521 ctgttcgtcc ttttggtaaa gtaaaatcag ttcctggttt tgcgtccaaa atcggcagtg
     2581 ttttaggatc cttttctatt ttttccgatt gctctgttgg cttatctaaa gaagaaaatg
     2641 caggttgtaa cgattttggt gcggaaatcg acggtttact ttcagacgaa cgttgccccg
     2701 gttgttgcac agaacgattt cctgatagag tggctaccga cgaattagga acagagtatt
     2761 ggttcaataa ctgctgcatt gctggataac tcccattttt cgcctgattt ttcacttggc
     2821 gaatctgttg tgaaagagaa ccagcagctg ttaattgtgg cacgggtact tttccagaag
     2881 gcaacggaat cgattgacca acaaaagcga tacctttatt taacaatggt tttctttgat
     2941 tattcccttg ttgcttactc aattgtgcca atgattgtac ctgttgtttt ggtcttactg
     3001 aattcatgga cgaatttgga atcaaaccat ttgaaagacc agaattccac tctgttttcg
     3061 atttggtaga agacatttca ggctctaccc catgataacg aacatgacga ccactataag
     3121 gcaaggcttg atttgaacgc ccacctagtt caaagggatt aaccacttgc ctccctttta
     3181 ccgattcttg tggtgtacgt ttttgttgaa acttttgaaa tccatgtatc gctgcagaag
     3241 cgagtggatt cgttgctcga tacacattcc ctgctaatcc tgcacctttg attgccaaat
     3301 tttgtccagc agttgatagt tgaccaggca gttgtttcat ttgagacaaa gaatgacttg
     3361 ctatgttttt cgtgttgttt atagtttgat ctgcagctcc taaccctagt ttctttaata
     3421 aatctttccg ttttacaatg agtaaaattg ataggagtgc tttgacaaac atatttccta
     3481 aaaaaccaaa aacagtattc attggaatta aatcgtctac tatgttatag atcaaaaatg
     3541 ccaccaaaat aatcacactg taaaaagatt tttgaaacat caagcccaaa aaggacttta
     3601 aggtattcat gagcatataa tcaaatactg gaacaaaaga agcgagtaat gaaaaaggaa
     3661 tcacaagtaa taaacacaac accaacattt gaaagataaa acgaatcatg ccaatcacta
     3721 caagtggtaa tccaatactt agattaagca atggcaccat agaaccaacg actgctttat
     3781 aaacagcttt agttggttga atatagacac gataagcctt ctttttccct ctatcttctc
     3841 ctgcagcagt ggtcactttt gtctcaatat cagataattc ttcacttgac gcttccttgg
     3901 ctagaaattc ttgtggttca atctctgctt gatacaattc ttctaaattt gttgtcccat
     3961 aatttaacaa tatatacgga tcaacaacag ccgctttata ataaagttgg cgcacttttt
     4021 gaatggcttc cttttgtgtc gttggcacta aaacaccatt gacatctttt gtcgccaatt
     4081 catctagact atttgtagca gaaaaaataa agccttctac ctccacggac aaatcattta
     4141 accctttggt aaaagcttct cctttactag gtacgttact accatagcca aaccaaacca
     4201 ttgaaaaaat gacaacgaag agaaatcgaa taaacaccat ttttgcgtaa tgtttccctc
     4261 taaatatacg aagagtaaat agatataaca catacaagac gacaatcccc gcaccaatag
     4321 gatcgtatat ttttgaataa atagtatccg aaaaagtgaa aaactggtga attaactcgt
     4381 tcattgcagc tccttcatag agcttctcaa tgacaaaatc aaggccttgc caaatcaatt
     4441 tacttaaact aaagaacata ttcgtcatca tttttagaaa atctgtgcca tttgccccaa
     4501 ataacatttt gcctaatgaa aattcactat cagataaata agaagaaaag gtatcaatat
     4561 gaaatttccc aatctcatag atcgattgat ccacctgctt ttctgccgca tacaccaatc
     4621 caccaacaaa aagcagtccg aatccaatcg ttaaaaaaaa caaaacgcac catttctttt
     4681 ccttatgctt tatataagcc attgtttcac ctcatttcta ttgcatgatt aacgtatcct
     4741 gttcttctac ttgagagtga cttgtttttt ccgttgtttg taatgcagcg gtcatttctt
     4801 caaacaaact gtgtacattg agttttccta cacgtccata gggatctaac atccaacact
     4861 gtcctttaat catgtggctc atttcagcta agttcgcttc atttacttcc aaacgtaaat
     4921 gattcaaaat cttttctcgc tcactgtctt catcaaaagc aaagacgaca ccaaaattcc
     4981 ctttttcttt atctgaacct aagtcaccaa taaattgtgt atctaacact aattgattca
     5041 tctgggaacg ccccacacgt gccattgctg caataatact acgtcctgcg ttcgaattat
     5101 taaataccca tgcttctgta aaataaatcg ctgttttctt actactatct cgttttccaa
     5161 acatttcaca aaaacgacct aaactaatca tcaaacacaa agactttcga tccgcatctg
     5221 tatatagttc tggtcggaca ttgtcatttg gtaatttcaa atcttggatc tctaaaatcg
     5281 ttgttttggc attaaagtct aatcccgggt tttctccata agaaaatcct aaacgtaaaa
     5341 tcgaatcttc taccgtcaag cgtaaaaact caccatattc tcgaacctct ttttgttcca
     5401 tttgttctaa atagtcgacc acatgcatca agccaacttg ttgtccggct tctttttgaa
     5461 ctaaaacttc ttggattccc tttaataccg ccccttttaa aaggtgattg gtacctaacg
     5521 gacttaactc atcaatcata tcctgggcta cttgtttcgc atctactcgg gataaacaaa
     5581 caattggatc taatacacca taattacggg gatttgtagc atctaacgtg acataactca
     5641 ttttttgcaa atgttctaca aacaaagggt aatgtttctg ataatacggg tcttgaatga
     5701 gtttcgtaaa ccatttgcgc ttttctcctt tgggatccac aaataaactt tgtacattca
     5761 taaacgaact atacaaaaag agcaagccaa ctaaaaaggt cttcccttta ccggtttttc
     5821 cagttactgc tacatgaggc gcatcaaaca acgcaccttt gattccttcc gcaatggcta
     5881 gaagattcaa aaagacaaaa cgatttgacg cataaattaa ttcttctaat ggtgtttttt
     5941 ctcctcgaga accaccatcc cctaatgtat cttgccgacc aatataccag ccggattcca
     6001 tacctaattc tgatactgct gaaaacaacc attcgccaac tgcttctaca gttgttcggt
     6061 gcttccaatg taactgccgt cccaatggtt gccctaataa acatttatag aacaattctc
     6121 gttgatcatt ttttgcacgg tggatatgga taatcgtatg aagttgttga ataaccgttg
     6181 cactattttg acgaacttct tcaaccgttt gaccgtatac aataaaacat gcgatccaat
     6241 cgacgacagg ttgcccatta tctactcgtt gctttaaatg ttttactaat cctcgaacag
     6301 ttaataagcg ttcactaggt tcttcatcat tttgaaccaa ttccttatgc tctgttttga
     6361 accgttccga tagccaattc agttttaaac caatacctcc ctgtcctttc aagcgtggat
     6421 agtgaatttt cattcgcaat tctgctggga aagaaagatc ctgagcaaat tgaaatagat
     6481 cacaatttct catattttca ggtgtttccc caataggcaa caatgctaaa taactactcc
     6541 ctaactcatc ttctaattga agaaggcctt tatgttcctt cggtatacgt acatacgttc
     6601 cttgattgtc tttcagtact tcccaattat tttgttcttc tactttgtga gccattcctc
     6661 gaatatattg cagacgattg agatacaaca gctctttttt tactaaggga cgaatagaaa
     6721 agctataaag taattgtgtc acttctttta cataaggatc actttcagaa aacaaagttt
     6781 ccaaatcatc tacttctaat ccaaataagc gtgctaaact tcctgctaaa tctaaccaga
     6841 ttagatttat tacatcatga aaactctcca ccgttgcttt ttctttgact ttgattccta
     6901 aaataaatga aggacgatag acttgacctt ctcgttccaa caatgtcact gtttcttttg
     6961 cataatacgc accaattgct tgactatcgg gagaaaatcc ctttcctaat gtgtcaaact
     7021 tcgtccgtaa attcattgtt cgtggataca tcaccaactc cacatcttca taatctgcta
     7081 aaagcaacag taaacgagcc atcatttctt ttatttcctg tgtctttttt ggtgtgtttt
     7141 ttgaaattgt tcgtggaaaa acatgatagt aggcccagat acaatcctct ccatttaaca
     7201 aacaattttt cttgaatgct tttactggtt cttttacaag caccaatgaa tttcacctcc
     7261 caataaaaaa ggaacaacca gttgattgtc cctttttcta atcctctaaa taaacatact
     7321 tattcacaaa aagtaattct tccaatgcat tgtttgatac cttgtcccag ttataataca
     7381 cacaatcacc attcatcata cgaatcatat aacaaaacca atcccataaa taaaaatgca
     7441 ctttttttcc atcaggtttt agttttgcca gtgctaacac acataaatag gggagtccta
     7501 tataaaaaac aactcctgct ggtggaaaaa aagaaatgac ccaaccaata aatgaacgta
     7561 aaaataaata ttcaaagatt acgaccaaca aaaaagttgc tgcattgact aatgcaactg
     7621 gaaatggaag acgccaattg cttgtaatct gttgtacgcg ataaggttcc gcataaacaa
     7681 tcgaataatc ataaggttcc caatccactt cactatcctc ctaatccata aataattttt
     7741 caatgatttt accaaacgca tcaaatacac gtgttggatc atttaagaag aaataaactg
     7801 cggcacctgc tgcgatagat attaatgcat ttcctacttt tgatttcata aaatttcgca
     7861 atcccagata aacaaccacc attattaaaa aatagccacc ttgcgttaat ataaaatcta
     7921 caattccctt cagtgtcatt tccgttcttc tcctttccta tccaaccaat tatgtgttaa
     7981 tttttcaaca taatactgat ttgttttttt cgtaatgaca atcgtcattt gttctttttg
     8041 taaaagtgac gtatcaccat ctcgaaagac gaccgttgct tttacaataa aaggtttttt
     8101 cgtttcttta taacgcaata ctttcatctc tacttgatca actttagctg tttgattgag
     8161 tccttcaggt tctttcatca tgtatgacat atcttttgca ttgtttttcg catacttatc
     8221 taagaaatct tccacaaatc cttcaacatc tttcgcttcc gacgcatcaa ccgtttcgta
     8281 taatgtgtca tcataagtaa tcattcgttg tttttctgta ttctttggta gtgccgtaaa
     8341 ataaggataa gcaataatac gacagccctt gtttgaaata gaaaagggaa gattgagtac
     8401 ttgtgtgatt gtcacacttg tttctttctt actctctgga ttcatcgagc gaatatcata
     8461 agttacttta taactagcag ttggaatggt ttcaatttct tttaatccaa taaacgaagc
     8521 agcagttaat tttcgaacac ctgaaacact tcctggttct tgaataaagt tttgtgcata
     8581 aaactctttt tttaatgttt cttgacgttg ttgttcttgt tcatgattat cgaatgtatt
     8641 catatacaaa gcaacaaaac gattcataaa ggattcaatt tcaggtgtgt acgttaattc
     8701 tgaccgactt tgtacttctt tcttttgtaa ttgttggatt tcatctttca aaaacgacac
     8761 ttgaaccttc gttttcatgg attgaaaaaa tgccaagccc cctgaaaaac aaagaaataa
     8821 aaccaaacac caaatccatt tcactgcttt ttttgaatgg attttctttt gcttcggtac
     8881 tttctctgta taaacttttt ctttccgccg tagttgtcgt tttttcataa gctactcctt
     8941 ctagtatgtt tttactagta cgtacgattg acaacgataa gcttgttttt ctgaaaaata
     9001 gtccattcaa ttaccctcat tttctattcc ttttctcttt cttttatatc caacaaatcc
     9061 agcaatactt gtcaacactc ctcctagaac agaaagtgca acatgtattt cttcaccagt
     9121 ctttggtaag acttttgttt ttccatctgc tgttttgatt tcaatattgc cacttggaag
     9181 tttctttaca ggaatcgaca aatcctttac caaattccct tcactatctt ggattaatgg
     9241 aataccatct tttacaccaa caattgtttg atcacctgct acttttaatg gggtggttgg
     9301 tgttggaata tgggtcaatt cctctacatt actagtgaca atcggtacac tcattccttg
     9361 agaagtattc gtcgtaattt caccgcttgg ggttaccgat actgtgactt cttttgctgg
     9421 aacaataggt ttattttctt ttggaattgc ttctgatttt tcaacaggtt gtttcggttc
     9481 ttctttttgt tcttcactgg tagacgacat cgttggctgt tccgtacttg acgaatcagt
     9541 attcttttca gaagaactgg ttgttcctga ttgttccgga gttgtgacta tacttgaatc
     9601 cgttgttgtt gaactatgca caccttcttc ttcgatcgaa tcagtcggta aagttgattc
     9661 aggaaactct ggttctggtg tcactggctc tttttcaggt ttctctgact gcaattgttc
     9721 aggttctgct ggtttttccg ttgttcctcc atttagctct gtctcaacat ctggtaatcc
     9781 ttcccctggc aatgtttcta caatttctga cttaggtgtt tctacagttt catctgcata
     9841 aaccaatgaa ctccctaatt gaaacgaaaa taatgaaatt cctagtgttg ttacgactgc
     9901 taatgttgtt tttttcatga ttattcctcc aattttttat gcaattcgtg ttttgttcat
     9961 agacgccgca ctaatattat ctgaacgata aaagaactcc cgtccgtttt cttctaaccc
    10021 aacatatgga ttatttttga ataattgaat caatagttca ctggtgttgt attttggata
    10081 gtttgcaaaa aaataatact gtgcttgtcg cccatccgaa aattcaacaa gtaattcata
    10141 acgataccgc ttcaatcctt ttcgttgttt taaaggatct ctataccaac ttgtccatcg
    10201 ttccattcgt ggatagagat agacacgata cacatagaac aatgccatac cacataccac
    10261 caaaaataat aatcgccaca taaaaattcc cccttttctt ctacctttct tgaatcatcg
    10321 aacattcata ccctctatag agatttgaaa aatccatcat caattgttca tataaacgtt
    10381 gttgcaaata gcgattgttt cgaatacata attcttcata gccatcaatc acttgatctt
    10441 ctttttcata gcatcttgta gctctatgac gctcagcttg catgacataa ccaaactctg
    10501 caccatatcg ttctactgtt aataactgtt gactacgcaa taatgcaata taacaatcac
    10561 aaaaatgttc aaccgacatt tgatttttct cacgtttgtg aaataggtat tctttagaaa
    10621 gcacattatt cgttaaacaa tctgataaat agcgataatc tggcggtcgg ttattttcat
    10681 ctgtgaattt ttcccataat aaactgattt ttattcgttc agcggctaga tagtcttgtt
    10741 cgcgtggata aaattcatca atcgcacaaa gttttttatc acctgaataa acaaatccat
    10801 ataatgcgaa acgaactctt ccttttcaat tggctctcct aaaaattcca gtgacatacc
    10861 atcttctcct tcctaaaaaa aataaaaaag cagaaaccga tttggcttct acttttcttt
    10921 ccttttatag ctctttttta cggaagaatc cgcaaatgac attttttcaa aaaatcaatc
    10981 ggcacaagtt cacgaaaact tggagcaatt ttggatagaa tcacttcccc atcttgtaaa
    11041 atacaatcat aaaaatcatc agttacttga tacttacgct tcttacaata attgataaaa
    11101 tagtagcgat cattttctat ttccattcgt tgttctacta agcgcatacg aacatcatca
    11161 tagaaataag ccacaaatcg cccttcaagt tcatgttttt caatgggata ctcttctggt
    11221 aacaatctat tttttcgtcg tttatacatt gctggatacc ttagaagaag ttcatctaaa
    11281 cgtctgtcag tatatggtaa ctgacgaatc aacgattcta atcgttcttt atacagatag
    11341 aataattggc gcaagcaata gtgtaccgcc tctggatttt ctaatactgt tggatcacgt
    11401 gaccaatacg caccaacata aatcataatt tttttcattt agacaatcac accagcaata
    11461 ctaatgaccg atccacaata ggtagaacaa gtggcatagc gaccaatttc ctctaattct
    11521 atctcaatct cttctaaaga aagatatgtc tgttcttctg ttggctggcg actcatttcc
    11581 caagtgacag tcaccaatga aatttcttcc ataagaggtt tttcgctgct aagtgctgcc
    11641 acacagtttt catatatctt cattttatcg cagccttttt taaccgctaa aatcgcaggt
    11701 attggcaatg aaaatcgata aaagcgaatg aattctagcc ttcctttcct tcggctaaat
    11761 tccaactctt cgagttcttg ttcgtatccc ttacgaactt ctaataagcg tgcaatttct
    11821 tgccagttct ctttttcctg ataacgtcca gcttttaccg cttgttcaat ataaggaact
    11881 cgaaaacgaa aataagacaa atcaaccatc ttttcattta atgcttcgat tgcagccttt
    11941 aaatccaaca ggcgcagcca ttctgttgtc gtatatgcat tcatgcaatt ttccctcctg
    12001 acgttcccat ccattctaga tagttttgtt gctcgtcatt tagttcggca ttatcaatcc
    12061 cttcttgtac aaagtctctt cctaaaatac gatctgcttc ttgtaacaac tttaatgtcg
    12121 gagctacttg tttcaaaaac cagcgttctg aacgatctaa tgagaatttt tctggcttta
    12181 ctgtaaaatc caattcttcc atcgaacgaa ataattttgc ccatcgttta tcaacaatcc
    12241 gtgcatcact ttccagtcta tcccaaacaa taagttttgc attaattaaa ccaacgccaa
    12301 tctctcctaa acgtttcggt gaatcaccta aaataaatgc ttctactgct tgtgttgctt
    12361 tctcattggc taaccgtaac tcatatcgat tataaatacc atatttcgtt aatgcttcta
    12421 attctgaaat tttttctcgg ttcgcaattt caaaacgttt ctcataaaaa ttaaaaaaga
    12481 gaggactctt tcgcgatcca aaatacaaag ataatccttc acttcgaaaa tagccatcat
    12541 ctaaaaaaca accgccaata ttctggaatc tcttaaagtc catttctaat aaacctgctt
    12601 tttgtttttc tagtaattca aagagatcga aatgttcttc cgtttccaat ccccaaagtt
    12661 catctaacgc aatatcgaat cggctacatt gcacacgtgt ataaccagct tccaactttc
    12721 cttgattttt ccaaatggaa tatagccatg ttcgccaatc tgtttgttgt tcacgcatga
    12781 cttcctcata ttcccggcaa ccttgtcctg aaaattgcac aagtacattt tctgttacag
    12841 ttcgttttga atgccagaac acccagatat tcccacgaac agaaagacag tcaaaaagat
    12901 acaagcccgt tggacgtgtt tcaaattctg tcagatccat tcgtaataca cgttgaatca
    12961 ctgttttgta gtctgtccca aaaaaggaaa gacgcaaata atcaatcatt gctcgaagat
    13021 ctattccgct tttcatatac aatcgaaagc gttccttgat tcgttgttca aaatcctctg
    13081 ggatttttct tttcccttgt tccatctttc taatgtaatc ttttgagcag cccatcaatt
    13141 gcgcaaatct tgcttgtgat agcggctttt tatctccact tatattatga attgcactta
    13201 acttctttct gtagcttttt agctcaatcg gtgtcatttt ctcgctcctt ttataaaata
    13261 acaaaatcga ttttttttaa ccctttagaa acaccgatat atcaactagt ccccctcgtt
    13321 ttttgggaaa agtagtacaa ctgctgacct tagataatca cttgatacaa aagggtttat
    13381 aaaacttttt tctatgtgtt atgttatttc tgccccccat attatatatc ggggggcttt
    13441 acacaaagag gccgtgcgca agcgcacgac ctctttgtga gctgcacacg ctgctagcga
    13501 gtgcgcatta taggcccatg cttcgcagcc ttaatgcgca ccgccaggca gagtgcggct
    13561 accagactac gtctagagcc ctcccgatag ataataacta caaaattaaa tgatctaatt
    13621 ttgttatttc actatatttt tcaggagaaa aaacgcgata aattgacaaa gttaaatcct
    13681 cttctaaatc tactcgataa tgatacaatc gagtcaatcg ttctatatct tttttcgtta
    13741 gcaatacttg attaataact tttcctgtaa aaacacgttc tttgcgcaaa aagcggtaat
    13801 cagttacttg ttcaatacga ttcaaccaat cataaaacgt tctggaagaa agagaaggat
    13861 taatcattcg tattgctgta gctactctat aataacaact ctctgccatt taacttcacc
    13921 tccttagttg attacaaaat atgcgcatca gaaaaacttg ttccttttgg atcttttgct
    13981 aaatacattt ggcgctctag ttgttcttgt aacaactgat tatcatctcc catcgtttga
    14041 tcttgactat cctcttttac tgtaaaatca tagacaatca tttcagtaaa tttctcaaat
    14101 tctttgataa agtgataatc tttgtaaaca aatggcgcaa aaaactctac aggtacatca
    14161 aatcctggaa aagcatatcc acgaccaggt tgatctttgt tgtaaaatgg cttattttta
    14221 aaatcttctc caaaagtcat taaataacca atgggagaca atcgaccaac agaaatacgg
    14281 aatgttaaat tatcccgaac accacttgga aaatattctg catctggtcg ttgcattgct
    14341 aaaataatgt acatccctgc ttgacgaccc tttaatacta atggctgaat caatttatcg
    14401 actctatttc tcgcttccat atccaacgaa ccataaaaag ctgcccattc atcaaaaaag
    14461 ataaaatgcg gtggtaaccc ataataagca taatttttcc ctggttcata tttaggatct
    14521 ttcatggttt tcatataggc ataccgttga ttcatttctt ttaatgcatt tcgtaaacaa
    14581 ataatcattt cttcatttat tttgtaaaac acatgtcctt tgaatacatc gacgtcacct
    14641 aatgctgcta aatctgccgc ttttccatcg caaatatcta ctgtcccaac acttaaacaa
    14701 gcttgaatta aagaatacaa taaataggtt ttcccaccac cgattgcacc agcaatcaac
    14761 atatttggtg attgatcata tacccaatcc aaagttttca ttaaatgaat acgtccattt
    14821 tctgctatac attgatcaat aggaatccga ccagaatctg aaaggaatac ataattgatt
    14881 cgggcttttt ctcgttcttc acgaattaaa tcacgcaaaa atacttcaga taacaaatga
    14941 cccattttta aaaatcgttc gtgaaaacgc ccaccatcta aaggaaaaga aaccactaat
    15001 tcttgtttct tttgataaaa ataaactttt ggtaatctca ttttttccct tgtttgtttc
    15061 tcactaactt gtttctttgt ataaacaaac cgattgtcaa ttaacattcg aacaagtaac
    15121 tgacgccaat aaacagaggc aaaatatcct tcttttagtc gcgaccaatg catcaaccct
    15181 aaaattaacc cactaaccag caacaaatac aacacaatac ttattccagg aataagatac
    15241 atttgccaat tcgaaaacgt acttaaacgt ggataaaata ctttccaact aaaccaacct
    15301 aacagaatca aaaatggaaa caaaaagaaa ccataatagc aaaaccataa ataacgattg
    15361 aaatagcgaa ttcgccgacc acgatacttg aaaaaatgag ct
//
LOCUS       DACDCJ010000070        14397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDCJ010000070 DACDCJ010000000
VERSION     DACDCJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.070"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            85..603
                     /locus_tag="IV038_002152"
     CDS             85..603
                     /locus_tag="IV038_002152"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP8342345.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            731..1414
                     /locus_tag="IV038_002153"
     CDS             731..1414
                     /locus_tag="IV038_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP8342346.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1723..1796
                     /locus_tag="IV038_002154"
     tRNA            1723..1796
                     /locus_tag="IV038_002154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1757..1759,aa:Arg,seq:tct)
     gene            1955..2314
                     /locus_tag="IV038_002155"
     CDS             1955..2314
                     /locus_tag="IV038_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP8342347.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2437..4068
                     /locus_tag="IV038_002156"
     CDS             2437..4068
                     /locus_tag="IV038_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP8342348.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGELVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4130..4771)
                     /locus_tag="IV038_002157"
     CDS             complement(4130..4771)
                     /locus_tag="IV038_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP8342349.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            5053..5361
                     /gene="rplU"
                     /locus_tag="IV038_002158"
     CDS             5053..5361
                     /gene="rplU"
                     /locus_tag="IV038_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP8342350.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5388..5732
                     /locus_tag="IV038_002159"
     CDS             5388..5732
                     /locus_tag="IV038_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP8342351.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5745..6038
                     /gene="rpmA"
                     /locus_tag="IV038_002160"
     CDS             5745..6038
                     /gene="rpmA"
                     /locus_tag="IV038_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP8342352.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6131..7279)
                     /locus_tag="IV038_002161"
     CDS             complement(6131..7279)
                     /locus_tag="IV038_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8342353.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7291..7602)
                     /locus_tag="IV038_002162"
     CDS             complement(7291..7602)
                     /locus_tag="IV038_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342354.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7655..8050)
                     /locus_tag="IV038_002163"
     CDS             complement(7655..8050)
                     /locus_tag="IV038_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8342355.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8086..8430)
                     /locus_tag="IV038_002164"
     CDS             complement(8086..8430)
                     /locus_tag="IV038_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342356.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8731..8952
                     /locus_tag="IV038_002165"
     CDS             8731..8952
                     /locus_tag="IV038_002165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342357.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9079..9855
                     /locus_tag="IV038_002166"
     CDS             9079..9855
                     /locus_tag="IV038_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8342358.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            9852..10046
                     /locus_tag="IV038_002167"
     CDS             9852..10046
                     /locus_tag="IV038_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342359.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            10048..10332
                     /locus_tag="IV038_002168"
     CDS             10048..10332
                     /locus_tag="IV038_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342360.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10353..10541
                     /locus_tag="IV038_002169"
     CDS             10353..10541
                     /locus_tag="IV038_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342361.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10867..11106
                     /locus_tag="IV038_002170"
     CDS             10867..11106
                     /locus_tag="IV038_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342362.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            11112..11798
                     /locus_tag="IV038_002171"
     CDS             11112..11798
                     /locus_tag="IV038_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342363.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11801..12631
                     /locus_tag="IV038_002172"
     CDS             11801..12631
                     /locus_tag="IV038_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8342364.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            12648..13499
                     /locus_tag="IV038_002173"
     CDS             12648..13499
                     /locus_tag="IV038_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8342365.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13496..13855
                     /locus_tag="IV038_002174"
     CDS             13496..13855
                     /locus_tag="IV038_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP8342366.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            13870..14031
                     /locus_tag="IV038_002175"
     CDS             13870..14031
                     /locus_tag="IV038_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8342367.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            14028..14333
                     /locus_tag="IV038_002176"
     CDS             14028..14333
                     /locus_tag="IV038_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342368.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT         4833 a         2338 c         2851 g         4375 t
ORIGIN      
        1 aacattttta cagctataat ttgaaaaaaa tttatcgtta ttatatgatt aagaaggatt
       61 gaataaagaa agaagttgaa aacgatacct gattacaaaa aaataacttt tataagttct
      121 ataacgttga ttatattttt aattcttatt cttctcgtcg tcttggttcc ccgaattttt
      181 ggtttgactc cttatattat tgaggacaaa gaaatgagtc catattatgc acaaggaaat
      241 ctgatatatg tacgaccgaa aaatacaaat gagattttag tcggagatat tattacatat
      301 tatgaaaatt caggaaaaaa aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac
      361 gaagattttt atacaaaagc tgatacaaag acttatattg agcctggagt agtaaaaaag
      421 agaaatatta tcggtagtcc catctttcag attccatata ttggactagt cctttcaaaa
      481 acggcatttg catggattca ggggttattc tttacgattg ctttttgcct aacaggaatc
      541 acagcatgga atacctttgt agagttgaaa aagaagcgga gacgaacaca gaaatttttg
      601 taagaatgtt aaaaacataa atgtattcca gagatccaac gactcttctg aacatgaaac
      661 acgagaaaac actcatgcag cttgtagatt tagtcagttg ttcgtataat gtgtgaagag
      721 gaggagtagt atgcgcaatg aaatgatgca tcgtcctgta gtcaaagaag aactattgga
      781 ttttatgcgt acccagcaaa aaaaattacc gggtgagcta ggcaaactgg aagaagaagc
      841 tcatgtagca gaagtaccag ttattcccca tgaaacagtt gtatttttga aatttttact
      901 tggacagctt cagcctgaac gaatcttgga gatcggagca gctatcggtt tttcatcaag
      961 tgtcatggcc acgacaatcg gagaaaacgc tcatgttacg acaattgatc gtttcgatgt
     1021 catgatcgag aaagctaaaa aaaactatga acgtctagga ctgacagata aagtgacttt
     1081 gcttgaaggt caagcagcag atatcttgcc ggagttgtct ggtccgtatg actttatttt
     1141 tatggatagt gcaaaatcaa aatatattga atttttacct gaatgcttac gtttgttaag
     1201 aaaaggtggc gttttgatgg tagatgacat cttccaagga ggaacgatct tgctcccaga
     1261 tgaagagatt ccccgtggca aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt
     1321 tgtgatggat catcccgatt tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt
     1381 aatgacaaag gaatcagaaa caatcgagtt gtgatacatg attatggcag cctgtagata
     1441 atcgattgaa tcgagggctc aattcgaact tgatgtaaaa gtttctatat ttgtaagtca
     1501 attaaaggat acaggaaatc taaataaaca ggagtagcat gaagtcaatg tagctcctgt
     1561 tttcaattga taaccttaga acagctgaag gagaatgtct cgttttaaaa gattgattta
     1621 gtagttgggc caaaataatt cattatttgc taattaaata actatttcgt attgacagaa
     1681 gcaatttaat tatctataat tattgatgtt ccgctgattc ttgtcatagt agctcagctg
     1741 gatagagcat ccgccttcta agcggacggt cgggggttcg aatccctcct gtgacgctaa
     1801 aaaaccgtta gaaactgagg tttctaacgg tttttctttg ttttttgtgc caatattgtt
     1861 tgttttcaaa ggcaaataaa ataaaaaaac atccttatcg agtacaatta taaggagcaa
     1921 aaaaataaaa ctcatcagaa aagaggaaga agatatgacg atactatcta gaattttagt
     1981 gactctagtt gcaattgaat ttttttatat catgtatatc gaaacggtga gaactgattc
     2041 tgacacgaca agtagggttt ttaaaatgag taaagaagag ttaagcagaa aatctgttca
     2101 aacattgttt aagaaccaag gagtgtacaa tggactatta ggagtgggac tactttatgg
     2161 agcttatctt tcttccgcat ctaaagaaat cacaagtatg ctattaatat ctattttttt
     2221 tgttgcatta tatggcagtt tgactagcga tcgttggatt ttactcaaac aaggtgggct
     2281 agctctgttg gcattgcttt ctattttctt ataaatatag aaaaaataag aatttatcta
     2341 acaggaaact ttgtgaaaaa tattacttta tctcatgaaa tatgatattc taatgaatgg
     2401 tattttagaa aatttcatga aaaaggagtt agataggtgt tcaaaaatta tgtttcctca
     2461 ctaaaaaaag aattttcagg ttatgatctt tcaagatttc agaaagatct tttggctggg
     2521 atcacagtag ctgctgtagc attgccttta gccttggctt ttggggtatc aagcggagct
     2581 gatgcggccg caggtttgat tacggctatc attgccggta tcgttattgg cggtttatca
     2641 ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg cagctatctt gatgtctatt
     2701 gcggcaacaa agggaatgca aggtgtattg cttgctactt ttttagcggg tctattttta
     2761 cttgcagcag gacttttccg attaggaaca ctgacttctt ttatcccagc acccgtaatt
     2821 acggggttta cttcaggtat tgctatcatt attgcattag gtcagatcga taatttattt
     2881 ggtgttcact cggaaggtac aaacgttatt gaaaaactaa gtagttatca aacgatagga
     2941 tttccgataa acacagcctc tttgatcatg ggagagctag tcattttagg gatgtttatc
     3001 tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc tggctatcat tttggcaaca
     3061 gcagttatgc tattgttcca tttaccagta gcaactgtgg gtaagattcc acaaacattg
     3121 atcagtagta atcgtttgaa tatgggagat tttagctttt ctgccttgca acaagtggca
     3181 gttccagcta ttagcattgc tttattagga atgattgaaa gcttgctatg tggtgcttcg
     3241 gctggccgaa tggcaaataa gcccttagat agcaatcaag aattaatggc gcaaggaatt
     3301 ggaaatctgt tgcttccttt ctttggcggg attcctgcta ctgcggccat cgcccgtaca
     3361 agcgtggcca tcaaatctgg tgcacaaact agattagctg gtatgataca tgcagttgtc
     3421 ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac agattccaat gccagcactt
     3481 gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat gggaaacgat caaggagcta
     3541 tttactaata aatattggtc agcagttcta ttgtttatcc ttacgatgat ttgtacagtt
     3601 atctttgatt taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt
     3661 gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg attggcaaag aatggggctg
     3721 ccagaaacgg aaaagctgaa taactggaca gtagtgtata ttagtggtcc actgtttttt
     3781 atgtcttcag aaaaactcaa aaataaattg gaagaactaa atcatcacga tggtgtcatt
     3841 ttctccatgc gaggcgtacc aagtattgat ttgacggcac gtgcagtttt tgatgaattt
     3901 caagaaaaag cagagcaaag aggacaaacg attatctata cttccttgca gccagaagta
     3961 gaaaaacaac tttctcactt gtgggaacat acattcacgc atcaacatca aacagttgct
     4021 catgcgttga cagctttaca tgcgaaatat actgcaatta gccattaaaa caatgaaaaa
     4081 gaacagacaa gactgtaaac tgaagatttt atggtcttgt ctgttctttt taatattgct
     4141 gtaaaagtga agctagtact tgccagtctt gatggataac aggtacaagg gagcgattgt
     4201 agaaaatagc tgctggatga aaaagtggaa gaattcgata gtttttttct gaccattcgt
     4261 aggcatcctt ttgttctgtt aattgtaaga caggccctgt atagagttga ccatgatttt
     4321 tggtaaccgt atattctttg ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa
     4381 ttaattttgg ctggaccttc atcagttcgt aatccaatat tggtgcatgc gcaaatactt
     4441 cggaacgagt aggtgtccga tttggataaa cagtttctga ctgttgtgtc cgtttattga
     4501 tccgatgagt cacgcgatac gggcggctcc ttacagcact ggtgatatac acctcttctc
     4561 gtgttaagcc agttgaagaa agtgcctgca tcaattcttt tccagcttga ccgctaaaag
     4621 gaacatgcgt atcaatctct gttttccctg gggcttctcc cacgagcatc agtttaggat
     4681 gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg gccgttcatt cttaaagaag
     4741 cttcttgtat cagttctttt gggtattcca tttatgctcc tcctttttct tttattataa
     4801 gaataaaaga aacacaatgc gactatttta tttggtagaa ctgtgaggat agaaaagcag
     4861 aagtattgct tgaatttttg ttttactttt tgattttaaa tttagttgac taacggttgc
     4921 tgttctggta agctagtact gttgtaattg tggactccac agctacaacc gctcagaaca
     4981 agtaataagc acagcatatg ctgtcacttc ggatggcgag tctaagtata tcagaaggag
     5041 gtgctttata gcatgtacgc aattattaaa actggtggta aacaagtaaa agttgaagtg
     5101 ggacaagcaa tctatgttga aaaacttgac gttgaagcag gagaaaaagt tgtttttgac
     5161 gaagttatct tagtaggcgg agaatctaca aaagtaggcg ctccaactgt tgcaggtgca
     5221 actgttgaag gtactgttga aaaacacggc aaacaaaaga aagtcgttac tttcaaatac
     5281 aaacctaaaa aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt
     5341 atcgacgcga tcaacgctta atagcgtttg agaaagaagg cctatatatg atcaaaggaa
     5401 catttaagcg aaatgacgca ggtcagatcg tttcattcac tttgaccggg catgctgatg
     5461 ctggtcctta tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg
     5521 tcaacgggat tgcctcatta gccggttttg agccgattgt tgaaatgaat gaagaagaag
     5581 gcggttatct ttataccgaa gtcacttcag gaatgacaca agaacaaaac aatattgctg
     5641 agattctatt agaaaatctt ttattaggat tacaatcgat agaagctgaa aactcagaat
     5701 atatccaaat taaaacaatt aatgataaat aggaggtgca gaccatgttg ttatctatga
     5761 atctacaatt atttgcccac aaaaaaggtg gcggttctac atctaacggc cgtgactcag
     5821 aatcaaaacg tctaggtgct aaacgtgctg atggacaaac agttacaggt ggatcaattc
     5881 tttaccgtca acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg
     5941 acactttatt tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa
     6001 aacaagtgtc tgtttatcca gtagctcaag aagcttaata aataaactgc tctatctctt
     6061 gtgtagcaag ggatggagct tttttgtaaa ttatgggatt ttttttgacc atacttttga
     6121 ccatactttc ttagaagtta atataatttg ttagtttttg tgttgtttct tcttttttgc
     6181 tgttagtaat gtgtgtatat gtgtccatag tagtttttat atttgcgtgg cccattcttt
     6241 cttggacttc tttgtgatct gcgcctgcct catatagcat tgatgcatgt gtatgtctaa
     6301 aaccatgcag accgatataa ggtaagccgg cacgctcacg atatatattg tatctttctg
     6361 ccactgactg attaattgtg aaattatttt cgtcattggt aaacaaataa ttagctttag
     6421 taaatcctaa ttgaaataag tactttcttt gttcgagctt ccaaaatttt agttgcttta
     6481 tagttttttc atctaaataa attattcgat ttgatttttt ggttttagga gtttcagata
     6541 tataatattt atctgaacga gctacagttt ttttaatggc catttcattt gttttaaaat
     6601 taatattgtc ccaagtgagt gctaatattt cgccgattct acatccgctg aatgctaata
     6661 gtctaaagag cgtatagtct ctgtttttaa aatatggatt ctgttcttga gatacttcat
     6721 ttagaaataa ttccaactgt tcttttgtat ataattttaa tttcttttct gttttaattt
     6781 gtggctttgg aataagtaaa tttaatgttg gattatcaga tgttaaacca atatttatag
     6841 catacttaaa aactttattc atatagttta tgaaaagagg gtactgcttt gaagttcctt
     6901 tttcactcca atcatttact accttttgac aatatgccgt attaatttta gaaagcttaa
     6961 tatttccaaa tttgggatat atatgtttat taaagattat tttagtcctt gaatatgaac
     7021 tttcttttac agtttttttg tagctttcta gccataattc tgctgcttct ttgaaagtag
     7081 tatttgtact tgtgggcatt cctgtttttt gtaattctag ttctaaccta gaaagtgctg
     7141 tttttgcttc tttttgtgtt ctaaatcctc gtttagtagt atattttttc tttccagtta
     7201 gcggatcaat accgagataa gttttaaagt accatgcttt ttcgccattt ttctttttat
     7261 attgttttat cattgccatc aatattaccc ctattcaaat tctaattctg ccatgctatc
     7321 attgaaaaat tcgatcagtc cttgttcctc tataaattca agccatattt ccttcccgtt
     7381 aattaatgga actccttttt ctttacgaat tgtttgatac aagttagtca cgacttgttt
     7441 ccacatttct aatacagctt tatctatttt cttatcatcc cagcgttttt tttggggtct
     7501 atttttgttt ttttcaattt gataattagt agctttgatg ataaatgttt cgtatccggt
     7561 tgtgcttttt tctaaccatt ctttgaattc tgtatatatc atttttgttc acctttctat
     7621 ttttgaacgt atgttctttt ttgcttaaaa aattttagag agctgcataa caatagatgc
     7681 gagcttttaa taattctttc acaagtggtt cgaagcgaac gcttaaattg ttatcttcta
     7741 taaatttcat gtaattgata ttgtgtggtt ctaattcaga ctttgcaata tatctatcta
     7801 ataatttttc aatcatgaag cgatcagcct catattccat ttttgaatgg agagagaatg
     7861 ctaagttata taaaaaataa ttatcatgat gtttagaagc gtgtcctaat tcatgtaata
     7921 aggttctttg tcttctatac ttagataaat tagcttttat tactatggta ttcatacaag
     7981 caacataatg gccatcggca tcaaggtttt cacgctcttc taccttaacg ccgagttcat
     8041 taattatcat ttcaatttga ctgtccaaat gactcaccta cttatttagt tatctttttt
     8101 ctctaaatat gcttcaatca tagctgatag tatttcccta tcattatcgg tgagcggttt
     8161 tccgtcgcta ctcataacag atgctaaagc ttcttccacg gttagttttc tttcctctgg
     8221 tgcaactccc gcgttaggat catctgtgcg tcctagcaaa taatctgttg atacatggaa
     8281 gtagtcagca actttttgca aagtttctat tttaggactt tgtgtcttcc atttataaat
     8341 agagtttttt ccaaatccta ttctattttc taattcagaa atagaaatac ctcttttttt
     8401 acagagctct ttaacccttt cgaaaagcat gtcaaatcaa ccttcctaga gcgtacgata
     8461 ataatttata caaaaagata gaaaggtagt tgacaaatct atccaattgt cttatattta
     8521 actcgtaagc taatttaata agctaacaag caacaagaaa tacacctaat aaaataaacg
     8581 aattcgaggt cggcaaacta agaatgttaa tttttagggt ttttctaggc ttatttaact
     8641 gtatataaat ataagacagt tggatagaaa tgtcaataaa aattagctaa ttttattagc
     8701 ttacatattt aaagaaaagg aggaaacatc atgaaacaaa ataaaaaaac tccttccgca
     8761 aaaggagctt taataacttt aaattttaaa gaccaaaagt ttagtgctgt tgttgatgaa
     8821 attcatccac aagagcatca aaaaaattct tttgcaactg acgagaaaaa gactcagaaa
     8881 cgctctgaga aagttcttcg aataattcaa tttctgttgg aaaagattcg tcattttgga
     8941 agtctatttt gaattcttta ttaacagtct tgaatacggt ttgtttgaat aaggcgtcga
     9001 catccatatt aaattcttcc atactatttt cacctcgctt tctattcaaa attatatcaa
     9061 agaaaggaat gagcaaaaat gaacacaccg caaattttta actttgaaca acatgaagta
     9121 agaacagtaa caatacatga tgaaccattt tttgttggaa aagatgtggc taaagttcta
     9181 gggtaccaaa atggtagtag agatattaac agacacgtcg atgtagaaga tcggcaaaac
     9241 taccaaaacg gtacttttga atcgcctaga gggttaacta ttattaacga atcaggtttg
     9301 tactccttaa ttttaggaag caaacaacca aatgctaaga aatttaaacg ttgggtgact
     9361 agcgaagtgt tgccagcaat tagaaaacat ggaggttatc taactccaga aaaagtagaa
     9421 gaagctttgc ttaatccaga tacaatcatc caattagcaa ctaagttgaa agaagaacgt
     9481 actggaagac taatcgcaga acaaaaaatt gcagaatacg aacccaaaat ttcgtatttg
     9541 gatagtatct tatcttctac agattcagta actattagtc aaatcgcggc tgattatggg
     9601 atgtctccac aacagatgaa caaactactt cataagctag gtattcagaa aaaagtaggc
     9661 aaccaatggt tattatgcaa aaaacacatg agacagggtt acacaaaatc tcatacaact
     9721 gagattccga aatctgatgg cggaacaaaa gttgtgatga atacaaaatg gactcaaaaa
     9781 gggcgtctgt tcatttatga gtcgctgaaa aaagaaggat atattccaga aatcgattta
     9841 ttagaggagg aatgacgatg tcacaagaat ttattttgaa agtcagaatt caattagcaa
     9901 aatacggaaa gtctcagaac tggttagctg atactatcgg aatttctaga ccatatatgt
     9961 cggacattat gaatgggcgt agaaaaccag ataaacaaat caagccaatt gaggcagcac
    10021 tagcagagtt agagaaggag aaataacatg caaataacaa ttccagataa tttagtagtt
    10081 tccgaattaa ctacacagat tacgaatgct gttcttaatt cattggacga acgattacac
    10141 cttatgaaca aatcagtaga gcttcctcca tatccaaaca aatcagaggt aaaaaaagtt
    10201 ttaggcattg gtgatgacaa attaacacat tggataagcc taggcttaaa aacacagcag
    10261 tggagcaagt tagacatcag aattgaacga tcggaactcc aaagattttt gaaagaaaac
    10321 tttgagttct aaaggcaaag gagaatgatt ttatgtccta cacattgcaa caagaacatc
    10381 aaattctagg tctgattaaa cagcgtagaa aacaattaca agatgatcgt gcagcgctta
    10441 gaaaatccga tgagctatca gatagacaag ctgaactaat tgcttctgaa cttgaggatt
    10501 tgagaatgct agaaataaaa aatagggaga ttagattatg aagaagacag acacactttt
    10561 tataggattc attttggggt tattagtgat tgtagcgcac caaagtatta tcggaggaag
    10621 cttgttcgca gcattgatgg ttttaatcaa tctgcttgac tcaaaagaaa ggagcaacta
    10681 tggcacgaga agaagcgcta aaaatcggta aagtgattgc tgataattgg tgggcaaata
    10741 gccgttctat tattttaagc aagcaacata tcgaaaagaa aaaagcatgg caacaaataa
    10801 aaaagtgact ccgccgacca aagcaatgag tcactcaaaa aatcataact aaggagattt
    10861 taacatatgg aaaatgaact ttccactcta gatcaatatt tggccgatcc tgattggggc
    10921 aaatcgaatt tacaactggt tgctaacaag tacgaaaaat caattgatga agcaattgtt
    10981 gcattgactg atgcagcttc cataaccgct gatgacagta aatggaccaa gagagacttg
    11041 ggcattctaa aacgaggcgt taattggctt gaagatcagt acagagaaga aacaaaagag
    11101 aagtgatatg agtgtttaaa ccactaatag attcatactc ggcggtacta aagaaattta
    11161 aaggtaacga cattggtgca acaatcaacg aagaagtaaa tatcgaacgg ctgaaaacga
    11221 tgtatgacgg atatgatggc gatcgagtca ttgaaattcg ctttattgat ccacgacggt
    11281 tcacagtaca gcaaagaaac ttcatctatg cgctcatagg cgatattttc atcgatacag
    11341 gcatgccaac ggacttctgg aaggaattct tctacttccg ttttgaaggt gtcacagggc
    11401 gcgaaataag cctcaaagac gaatcgaata caaccgtgag tgatgccaat atcttagcga
    11461 atatcatcct agatttcatc tttgaacatc atattccttt caaagaagga tatgagattt
    11521 taccagcgaa tcaagagtat tacttctaca aatgcattac aaaaagagtc tgctgcatct
    11581 gtggcaaaac aggagctgat atcgatcact ttgacaaagc gctgggaaga cgaaagcgca
    11641 aagaagttga tcattcagag tacacatttg cagcactctg cagaatccat cacacagaga
    11701 aacacaaaat aggtgtgatc aatttcaaaa ataaatatca aatcaaaggg atcaagttaa
    11761 accaggaaac aattaaaaag ttaaggatag gaggataaat ttggctgaga taagttggat
    11821 caaacttaaa actactatgt ttgacgatga aaaaatacga ttaatccaag ctgttcctga
    11881 gtcggatgcc atcatcgtta tatggattcg attactagtt ttagcaggaa agactaacga
    11941 cgatggtctg atatatatcc agaggaacat gccttatacc gaagaaatgc ttgctacatt
    12001 gtttggcaaa aacgtaaata cggttcgctt agcgctaact acattggcaa atttcaacat
    12061 gattgatcta agcagtgatg gactaattgc catcagtaat tgggaaaaac atcaaaatat
    12121 cgagggtatg gataaagtaa ggctaaaaaa tgctgaaaga aaccgtaaat acagggaaag
    12181 aaagagacag gaacgtctca aattggaaaa tgacgttagc gtgacgtcac gtgacggtac
    12241 agataaagat atagaagaag ataaagatat agataaagaa gaaaagaaag gtaagtattc
    12301 tgacgaacac ttacgccttg ctaaaaagtt gcaaagtaat ttaactgaag attttccaaa
    12361 agaaatgaac aaagtagata tcgaaaaatg ggcagacaca atcaggttga tggaagaaag
    12421 agataaagca tctatagaag cgattgagta tgtgatcaat tggctaccta caaatgaatt
    12481 ttggtttgga aatattagaa gtgctaagaa attgagagaa aaatttgaga agctcaaatt
    12541 cgaaatcaaa gcagacaaga agaatcataa aaagcaaagt caaaaactac agtacagcaa
    12601 tcctagtgaa tatgacgact tgccaattta aaaaggagat gcatcacatg gaaagcctag
    12661 caaatgctat ggagaaacta ataagaagag tattagtgca aagcggaaaa tgtccagaat
    12721 gtagcgaacc tttgtatagt tggcgagcta aaaataagga tggttcagaa cgttgtaaac
    12781 caacatgcat gagttgtggt tataaagcgt tacgtgtgaa agaggatata cagaccgaac
    12841 ggatatataa cgatagctta aaagcacgag cgttgagttt ttttcaaaat ggttcggtat
    12901 taacagataa aactttgttt aaatgcaaaa tggaaaatta tcacgtagtg gatcaagaaa
    12961 caaaaatcgc tttagaaaaa gcaaaaagct atacgaatga ggttctgctg aaccatcctg
    13021 cacatttcat tctatcaggg aaatcaggaa gcggaaaaag ccacttatca atggccacag
    13081 cttgggaaat acttgagcgc tcaaattatg acaagaaaat actttttata agctatcaag
    13141 aattattaga gcaaataaag ttttcttata acaatactga actgagaaaa gaaattgaag
    13201 gatcgcttat agccgatatt aaaacaactg atttggtggt ttttgacgat attggagctg
    13261 aattaggtag tggggtatca aatagtaggc agtttacaaa caacacgtta aacacgctct
    13321 tagaagccag acagaacaag gcaacgatca tcacaacaaa cttatctggt cctgaactaa
    13381 gagaagccta cggtgaaaga attgtttcta ggatatttaa gaattcagaa ggttatgcgc
    13441 tgaaattcca acaaacagca gacaagcgca taaaaccagt gaaaggtagt atcgcatgaa
    13501 taaataccgt aataaaaaaa ctgttcatcg aggtatcaag tttgattcta tcgcagaagc
    13561 agagtattac gatctagcct tgtggcaagc tgaagcaaat ggctggaaag taaaacttca
    13621 ggaaagattt gagctgatgc cgaaatttga actagacgga aagaagtatc gcaagatcga
    13681 gtatattccc gacttcacat tttataaaaa cggcaaactt gtcaaagtcg tagatgtcaa
    13741 aggaatgcag acaaaagact ttaagatcaa ggcaaagttg ttctgtcatc aatatcaagc
    13801 gccgttgata ttagctaaaa aatatcggaa tacgttcaag gaagagcgtt tttaacgagg
    13861 tggtccatca tgacaacaga agaagtgatt caaatgcgta ttcgaaacat tcagcgtgaa
    13921 attgacgatc tagaacgaac aaaggcagtg atggtcaatg aaacggcgag aaaggcaatc
    13981 gatttgcaca tagagaactt aagaagggaa attcgtagat tggaggaatg agcgtggata
    14041 agaaagcgac aatgaaacga attgctgaat taaccaagtc agaatcttgg caggaagaca
    14101 aagaaatagt tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa aagcctaaac
    14161 ggaaaacgcc gagaaaaatt gcaatctggc atggtgatcg aattctagta acaggtacag
    14221 ctgaacagtt atctgaaatt actggattaa gcaaaaacat tatctgggat agagctagga
    14281 gcttatggat tgattcaaaa ggacgacagt ttaggtatgt ggaggagaga taatggatct
    14341 cattacacaa tacagtgaca tcatcctcaa gaaaatcatg atgaagattc agaaaga
//
LOCUS       DACDCJ010000071        14028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDCJ010000071 DACDCJ010000000
VERSION     DACDCJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.071"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..765
                     /locus_tag="IV038_002177"
     CDS             64..765
                     /locus_tag="IV038_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP8342369.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            762..1883
                     /locus_tag="IV038_002178"
     CDS             762..1883
                     /locus_tag="IV038_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP8342370.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1898..3130
                     /gene="pepT"
                     /locus_tag="IV038_002179"
     CDS             1898..3130
                     /gene="pepT"
                     /locus_tag="IV038_002179"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP8342371.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3262..4170
                     /locus_tag="IV038_002180"
     CDS             3262..4170
                     /locus_tag="IV038_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP8342372.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4205..4477
                     /locus_tag="IV038_002181"
     CDS             4205..4477
                     /locus_tag="IV038_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP8342373.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4488..5534
                     /locus_tag="IV038_002182"
     CDS             4488..5534
                     /locus_tag="IV038_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP8342374.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5785..8394
                     /gene="clpB"
                     /locus_tag="IV038_002183"
     CDS             5785..8394
                     /gene="clpB"
                     /locus_tag="IV038_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP8342375.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8545..9216)
                     /locus_tag="IV038_002184"
     CDS             complement(8545..9216)
                     /locus_tag="IV038_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342376.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9209..9703)
                     /locus_tag="IV038_002185"
     CDS             complement(9209..9703)
                     /locus_tag="IV038_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP8342377.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9723..10943)
                     /locus_tag="IV038_002186"
     CDS             complement(9723..10943)
                     /locus_tag="IV038_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8342378.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11090..12925
                     /gene="lepA"
                     /locus_tag="IV038_002187"
     CDS             11090..12925
                     /gene="lepA"
                     /locus_tag="IV038_002187"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP8342379.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13121..>14028
                     /locus_tag="IV038_002188"
     CDS             13121..>14028
                     /locus_tag="IV038_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8342380.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYG"
BASE COUNT         4677 a         2482 c         2914 g         3955 t
ORIGIN      
        1 ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag aaggagtgaa
       61 gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt tgtccctgaa
      121 tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc attgatatta
      181 agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta tgaatcagca
      241 cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct tgcaaatggt
      301 ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg tatgggaggt
      361 tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag cggacaagaa
      421 cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct gcaagaaaat
      481 cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata tgaaatcatt
      541 gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat gttcgggcca
      601 tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa
      661 agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa
      721 atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc agcggacgca
      781 ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa ggagacccag
      841 tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg acattagacg
      901 ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg attgctaaac
      961 atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa gaaaaaatgt
     1021 atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac atggatatta
     1081 tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg gagagttatt
     1141 tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct gtggatcatg
     1201 cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt gattataccg
     1261 gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt gcacaaccag
     1321 cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag gttattttgc
     1381 cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct tatgaggaac
     1441 ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta ggacgagtag
     1501 gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa gaagcgttcc
     1561 aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg
     1621 cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa agagcagatg
     1681 tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc gctgggttga
     1741 ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt atcgagaaat
     1801 ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct gaaacaaata
     1861 caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc ttttgccacg
     1921 ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa cgactccatc
     1981 gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag agttaggctt
     2041 agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac caagcaatgt
     2101 tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag attttaatgc
     2161 agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga tcgtactaga
     2221 tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa attatgaagg
     2281 ccacacattg atcacaacag atggcagtac attactggga gcagatgaca aatcagggat
     2341 tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc ctcacggtac
     2401 catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata aattcgatgt
     2461 tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag gtgagttgca
     2521 gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa atgtacatcc
     2581 agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt ttcataatca
     2641 gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct tccatttgat
     2701 ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg atcatgatcg
     2761 tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt tgaatcagcg
     2821 atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca tgaaagaaat
     2881 cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat
     2941 ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct cttacatggg
     3001 tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat tcgaatttgt
     3061 ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag aattgaacac
     3121 aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg
     3181 tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc tcttttttga
     3241 agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa aagttcaggg
     3301 aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga aaagcagcca
     3361 gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga attagtcaag
     3421 aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg cacgattcat
     3481 catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt acttccaggt
     3541 ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga agaagctgct
     3601 gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc
     3661 attatctcta cactgacaat cggaatctta gcagatacag cagtttggat cagtcaagaa
     3721 gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt attgctgaag
     3781 aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg aaaatcccaa
     3841 ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga cgactcagcg
     3901 accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat tttccgattt
     3961 attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg cattacttat
     4021 ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac tcgaacggat
     4081 ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg attaacagtt
     4141 tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag ttggaagaag
     4201 agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg tgaattttta
     4261 cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct ggcgtgttcc
     4321 tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat tgatctctcg
     4381 aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt
     4441 acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg tcgtctaaag
     4501 gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt gccattattt
     4561 attatttaat caatcaatca tttgcagata tctttgccga attgctgtct accagtgttg
     4621 ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa gggcgctcaa
     4681 tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg ttcttgacct
     4741 ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg atctcggaga
     4801 tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg acagcactcc
     4861 atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg gtcatccaat
     4921 tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct ggtatccttt
     4981 tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat gttcaaatat
     5041 tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa tgggtggata
     5101 catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc caagacagga
     5161 ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg tatgtacttc
     5221 cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc atttcgttta
     5281 tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt ggttcctttg
     5341 aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca gtttcttcgc
     5401 ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt gtgtatgtga
     5461 ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa gagaataaag
     5521 aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa gaaaaacgtt
     5581 atggataaga taattttacg tttgtttggc tcttttttta tatacataca atcaattttg
     5641 tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac
     5701 caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa gtgatcttac
     5761 ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac gcttcaagaa
     5821 gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat cgacattgcc
     5881 catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta tacggatgcc
     5941 ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga gtatccagtt
     6001 gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt tcgattattg
     6061 aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt
     6121 attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa taaaaacgga
     6181 ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga aagagtgacc
     6241 tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga tttagtacag
     6301 caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc
     6361 atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga acccggtgta
     6421 gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa
     6481 aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc tggagcaaaa
     6541 ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat
     6601 ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg aaaaacagaa
     6661 ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga attacatttg
     6721 atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa agcgttggaa
     6781 cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat cagtattctg
     6841 cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga caacgcatta
     6901 gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc ggacaaagca
     6961 atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc tatgccaacg
     7021 gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa
     7081 aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa
     7141 cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat
     7201 tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga agatgcagaa
     7261 aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa
     7321 aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat ggttcaagag
     7381 tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat ccccgtaacg
     7441 aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact tcataaacga
     7501 gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg ttcacgggct
     7561 ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc aactggtgtc
     7621 ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga agatcatatg
     7681 gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct agtcggagct
     7741 cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat
     7801 ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc
     7861 ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt agtagatttc
     7921 aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct ggaaggcgtg
     7981 actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct attacgtgga
     8041 aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac accattgagc
     8101 ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca acgtctggaa
     8161 catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct
     8221 tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga agtagaaaca
     8281 ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt aacgattact
     8341 ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc
     8401 attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct ccttttttca
     8461 aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc
     8521 ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt tataactttc
     8581 aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc
     8641 tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag
     8701 tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa
     8761 ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa
     8821 caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata
     8881 gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat
     8941 gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta
     9001 aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat
     9061 gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga
     9121 atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag
     9181 taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc
     9241 tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca
     9301 aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag
     9361 cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg
     9421 ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc
     9481 catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa
     9541 tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat
     9601 agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca
     9661 aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt
     9721 tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc
     9781 agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa
     9841 ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca
     9901 tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa
     9961 ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac
    10021 cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct
    10081 ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt
    10141 acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct
    10201 tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca gtagaacaga
    10261 acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt
    10321 tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt
    10381 tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc
    10441 ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta
    10501 tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa
    10561 gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc
    10621 aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc cattttataa
    10681 cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt
    10741 tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa
    10801 gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag
    10861 gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt
    10921 tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag
    10981 caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct
    11041 tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa
    11101 gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca
    11161 tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga
    11221 aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa
    11281 attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt
    11341 gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg
    11401 tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa
    11461 tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct
    11521 acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc
    11581 tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca
    11641 gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat
    11701 ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg
    11761 tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac
    11821 agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt
    11881 cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac
    11941 gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt
    12001 ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga
    12061 aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg
    12121 ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt
    12181 ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa
    12241 taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac
    12301 gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt
    12361 cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt
    12421 agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt
    12481 tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg
    12541 ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc
    12601 attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa
    12661 actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca
    12721 aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta
    12781 tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg
    12841 gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat
    12901 ggatgagcaa gatagtgtga aataacttat aatcaatcga aaaacctatt aaataaacct
    12961 ttttgagtag atgcaacact gaggattatc tgtataaaag attttaaact gtcatttggt
    13021 tatcacggcg atactaaaat taacaaaatt ggaatatttt tcttgaagat ttttagctgt
    13081 cagtatatga tgaaagcatc tcataggagg tgcttttttc atggttgata attcaagtgg
    13141 aagggttctt aaatctctaa gaaaagagaa aaaactatct caaaaaaagt tagctgattt
    13201 agcaggtatt tcacaaagta ctttagttaa atatgaaaaa gggtcaagaa aaatccctaa
    13261 agacgtagat aatactctaa gcaagattct gaatatagaa actctaataa aagatgagga
    13321 agataagatt gagattctca tcggtaaact aattgcatac agagatatga ataagttact
    13381 taacaaagag ttagctgata atataggaat tagtgaagta ttactcagtt atgtgttaaa
    13441 tagaaagaga aatccgtcaa aagaaatgca aaaaaaaatc gatatttttc tattaagtaa
    13501 tgaaaaagaa attttgaagg agattaatcg tgatagtgag atatttagtt tatcaaaaga
    13561 cgataaaatt gttatgggaa aaagaatacg agaagtccga aagaaccggg aagagacgct
    13621 tgaaaaattt ggtaaaaact ttaccatcta tacaggcaaa aatgttataa gtagatggga
    13681 aaaaggtatt aatattccag atattgaaaa actaatgaat attgcctatc ttggtaaggt
    13741 cactgttcct taccttatgt acggagaaga ttataagaat atacttccaa aagatgaaag
    13801 agttagcgat tttaaaaaga taaattcttt tagtatggga ttaaggatga ggaaaattag
    13861 gaaagattat tatctagaaa gggaggagtt tggaaagctt ttttctcctt caatttcaaa
    13921 atggtccata gataggtatg agaatggtag agatataccc aatactaatc gaattatcca
    13981 atatgcttat ataggaaatc tttctttaga atttttgatt tacgggat
//
LOCUS       DACDCJ010000072        14027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDCJ010000072 DACDCJ010000000
VERSION     DACDCJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.072"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1291
                     /locus_tag="IV038_002189"
     CDS             <1..1291
                     /locus_tag="IV038_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP8342381.1"
                     /translation="FLNLCDSNYEVILIDKKSEISYLECGTSLFIKREVSNLEDLFYQ
                     NRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQEYDKLILATGSKQIMLDV
                     PGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLF
                     DVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSM
                     DMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRS
                     SVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSE
                     SIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIE
                     EQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            1403..2854
                     /locus_tag="IV038_002190"
     CDS             1403..2854
                     /locus_tag="IV038_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8342382.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3203..3874
                     /locus_tag="IV038_002191"
     CDS             3203..3874
                     /locus_tag="IV038_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP8342383.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            4422..5861
                     /locus_tag="IV038_002192"
                     /pseudo
     CDS             4422..5861
                     /locus_tag="IV038_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6206..6373
                     /locus_tag="IV038_002193"
     CDS             6206..6373
                     /locus_tag="IV038_002193"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8342384.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            6632..6847
                     /locus_tag="IV038_002194"
     CDS             6632..6847
                     /locus_tag="IV038_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342385.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(7035..7322)
                     /locus_tag="IV038_002195"
                     /pseudo
     CDS             complement(7035..7322)
                     /locus_tag="IV038_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(7509..8420)
                     /locus_tag="IV038_002196"
     CDS             complement(7509..8420)
                     /locus_tag="IV038_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8342386.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            complement(8439..9443)
                     /locus_tag="IV038_002197"
     CDS             complement(8439..9443)
                     /locus_tag="IV038_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP8342387.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            complement(9440..11548)
                     /locus_tag="IV038_002198"
     CDS             complement(9440..11548)
                     /locus_tag="IV038_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP8342388.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNTQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            complement(11553..14000)
                     /locus_tag="IV038_002199"
     CDS             complement(11553..14000)
                     /locus_tag="IV038_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP8342389.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
BASE COUNT         4429 a         2570 c         2567 g         4461 t
ORIGIN      
        1 atttctaaac ttatgtgatt caaattatga agttattctg atagataaaa aatcagaaat
       61 tagttactta gaatgtggga catcgttgtt cataaaaaga gaagttagca atttagaaga
      121 tcttttttat caaaatagaa gagaactaaa ttctaagata aaaaagttgt ttttggagac
      181 agcagttgac aaaattgata ttcaaaataa aaagattatt tgtgtaaaaa aagatggcac
      241 aagattttcc caagaatatg acaaacttat actagctaca ggttcaaagc aaattatgtt
      301 agatgtaccg gggaataatt tagctaatat tttttcgata aagaattttc caaatgctaa
      361 agagattttt aaagctttag atgataatac tattaaaaaa gtaggaatta ttggagcagg
      421 atatattgga gttgaaattg ctgaagccat aagaaaaaga gggaaagaag tatatctttt
      481 cgatgttgca gatagagtgt tgtcgactta ttacgaccgt agtttttcag ataaagttga
      541 agaaatacta tcaaaaaatg gaatacatct ttgtctatca gaaagtccta aaaaatattt
      601 aggaagaaaa aagattgaaa aaatagtcac tacaagtggt acccaatact ctatggatat
      661 ggtcgtacaa gctataggat ttctatctaa tagtccaatt ggcaaaaagg aactcttgag
      721 agatgttagt ggagcatatc taactaatga ataccagcaa acaaatataa aagatatata
      781 tgctattggg gattgtgcta caactttttt tacgtccatt aatagaagta gtgttgattt
      841 ttctatttct aatgctctaa gaacagctta tattgcaact caacatattg acaatcaaga
      901 ttttacacct tctggttccc aatttacaaa tggcttttct atatttaatt acaattttta
      961 cgcagcaggt ttgaatttaa aaacagcacg attattaagt gagtctatag attatataga
     1021 aatagaagat ttcattagac ctgttttttt gagaaaaaat agtcgtgtaa aattaagaat
     1081 tagctttgaa aaaaatacaa agagaatagt aggtgtacaa ctttgtagtc aagaagattt
     1141 atctggacta ttatcaatgt tttctcttgc tatagaagaa caaattacat tagacaaatt
     1201 gaaaatgtta gattatttat ttcatcctag ttttagccaa ccctataatt ttattttaaa
     1261 ggcagtaaca ggattaaaca ttgaaacata atcgttacta aatgaaatca agaaatatac
     1321 tgtattactc aattcattga aaatatttat cataattttt aatagaaaac actattaaag
     1381 agaaaaaaga gaggtaaatc aaatgaaaaa atctttaaaa cttgcatcag cagttatatt
     1441 aatgtcccaa ctctttatag caacaccttt ccatgtgttg gcaagcgaaa attcaacata
     1501 caaaattgca aaagaaagaa ccgagacgac tgaaatgagt aattccgatg taacagcaga
     1561 gagtagtgaa cttttgacaa ctttatcaag tgaaagtagt acgacttcaa atgctgagtc
     1621 ttcaacaact tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc
     1681 gtcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag
     1741 tagtacgatt tcaagtgccg aatcttcaac aacttcaagt agtacaagtg aaataacttc
     1801 ttcgaaagtg acaagaccaa accatataga gtcttcaaaa aaggaagcgc caaaaacgga
     1861 aggacaaaca aaaaattttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc
     1921 acaaccaata ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa
     1981 ttttattatg aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc
     2041 ttctgttatg attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca
     2101 ggcaccgaat tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat
     2161 ggtaacaaac gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt
     2221 gtatgaaaac tatgaggaaa gttttgaaga ttatgccaaa ttattaacta agggaattag
     2281 tggaaataaa gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc
     2341 aactaaattc cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga
     2401 attaattaaa acctatgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag
     2461 taagactgga taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg
     2521 tggaaatgaa tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc
     2581 agttaaagct ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt
     2641 gatacaacac gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa
     2701 aactggagat aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag
     2761 tactggccca caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt
     2821 agttgatccg aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa
     2881 caagaaaatg gttggattct tagacctttg atcaggatat tgagactgga attgatatct
     2941 cgattacgat gagtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg
     3001 ccgtctttat gctcatattt tgagcaggtt ttactagatg aaaacaattt atctatcttg
     3061 aggatttcgt gtacgtatcg ttcagtcttg tattataaat tagtcataaa atcagactaa
     3121 gaaaaattga aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt
     3181 ctttcttggg agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga
     3241 gattactcat aatggatata aaaaatatcg ttacctatgt gactgtggga atgaaacgat
     3301 tgtatatcga tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa
     3361 aaataggcat aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa
     3421 gccaactaaa ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag
     3481 aaaaaagtta gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt
     3541 gaaatcaaaa aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc
     3601 tttggacaat aactgttcat ggaaaacaaa atgggtttgt caatgtgagt gtggcagtat
     3661 gtgtgagata agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa
     3721 aaggcaagaa acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt
     3781 accgaaaaat aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatggattgc
     3841 gtatataact ttaaaaaaaa gaggtactat ttaggatcgt atgattctat taagagtgca
     3901 gctcaagtta gaaaagatgc tgaaaataaa ttgtttcaac ctttgattga caaatatcga
     3961 ggtaatcatt aattattgtg gataataatt aacagtgata tataaacatc tataatatat
     4021 tattcatact attgacattt agtataatcc tatggaattg aatatcatag caatgtattt
     4081 gaaaagcaaa ttggaaaaca aataggtgca acaccataaa tatgaaaaaa ttgggaaaaa
     4141 gtagaatgga aattttctta cgtctcacga gatagctcac actattaatc tgaagaacaa
     4201 tgaagtcgac ttttagaaat tgctagaaga aagtaaagcg aattatcact agttactata
     4261 gagtaagttc aaataaagca tctcaaactt acatggttac atgagaataa caaatcgaaa
     4321 ttactcaagc ttcctatggc tagagtcgcg agaagttgtt gggcagtaga cataggttat
     4381 attgttctta gctacacgat cattagaaag gaaatcctat tatgcatagt tatcaagaag
     4441 atgcaaaagt ccaaaaaaat cgctggtgga ttttgattgc tatatctatg tttaccttca
     4501 tgtccacatt ggatggcagt attgtcaata ttgcactacc taccatctca gatgatatgg
     4561 gcataccaat gaatcaagcg gaatgggtag tgtccgttta tctgatgatt gtctgttctt
     4621 gtttgttgct gtttggtaaa attggcgata gctgtgggaa aatcaaagta tttcggattg
     4681 gtacgctgat ctttgtcttg ggctcgctgt tgtgtgggtt caatcattcg ctaggtattt
     4741 aattgttcgc acgggtggta caagccattg gtgctagtat gactatggct acaaatgccg
     4801 gaatcatcac ggaagtcttt cccatacatg aacgtggtcg ggcattggga gcaatcggtg
     4861 cgcttgtttc attaggctcg attgctggac caggtcttgg cggcttgatt ttgtcccaat
     4921 tttcttgggc gtacattttt tggatcaatg taccggtggg aatcgtaacg atgttgattg
     4981 ctgaaaaatt cttaccaaaa gatatcacca tgagcggcca tacaatcgat ggagggggct
     5041 ttgctgccat tgcggctacg attatcagcc tttttggaac aatctttttg gggcaagaaa
     5101 gtggcttttt ccatccagtc ccccttgtac tgttcgtaat ttcagtgatt gcctttgtca
     5161 gctttttaaa agttgaaaag aaagcagcct ctccattgat ttctttcggt atttttaaga
     5221 acaagatttt taccatgagc ttgattacag ccttgttgat tttttcttcc aacttctttg
     5281 ttaatgtagt gattcctttt tatctgcaaa atgctcgcgg gttgagtcct agctatgccg
     5341 gactgttgat gatggtcttt cccttgatga tggtcgtcgg ggcgccttta agcggatacc
     5401 tgaccgaccg ggttggcccg gagatttttg tattagtagg cttgggttta ttgtcggtaa
     5461 cccaagtcat gtatatgttt ttgtcagtgg actctccttt gtggctgtac attgtcgcta
     5521 ctgccatcat gggtgccggc aatgccttgt tccagtcccc aaataatacg atggttatga
     5581 gttctgtcac tatcgaaaat ttgggactgg caggctccat gaattccttt gcccgaaatt
     5641 tggggatggt catcgggatt tcgctttcta cgacggtctt atatcaagcg atgtctgcta
     5701 agatgggcca acgagtaact aactatatcg cagatcggcc ggatgtcttt ttatatggaa
     5761 tgaagattac ctttttaggc agctttgttt tgtgttttgt tgcctttttc ctgactatct
     5821 ggcgtatcaa aaaacaagga ggtattcaga aaaatgtctg aaaaaccatt gttgaagaat
     5881 ttttggaggc ctgcgtatgg gtgagtggga tttccatgga tgatggaaga tgaagtgaac
     5941 ttctgaaaac cgaaaaactc aagtattatc ttcaaaatac tagtactcaa ttagatgctc
     6001 gatttgatta caagaagact gcgaaagcca ctggcaaccg caagctaggt atccaccgat
     6061 ggactacgtt ttagaggcaa ctggttatga atacggagct aacgctctca tctgcataag
     6121 aatgcaacac ccagattatg taaagatttt cgatacttct gtcaaaaaaa tcatcaagat
     6181 agataaatga tttccgtcta gtcaaattgg ttcagaatgt ggacacacag atggtaagaa
     6241 gtcattcaca attcgagaat ggacttgtcc tatttgccgt acacatcatg atcgaaacgt
     6301 caacgttagt atcaatattt tgatcaaggg ctatgtatac aagcattgcc ttagaaaaca
     6361 tcaagaacct taggaacaac ggggatagat tggttaataa gagatatcgc tgttagtaaa
     6421 gacataggct tgcaagtata ctcttttatc aagaattccg tggctttagt catgataggt
     6481 ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct aattataata
     6541 aaatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta ccattttcaa
     6601 agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg atatgaattt
     6661 aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta ctggtatcaa
     6721 tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc tgaatctttt
     6781 agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga ttgaaatatg
     6841 gagataatta tcgtctagtg aactacttgc cacttagctg aatttaacga tccttagtgc
     6901 ttgaagtggg agattcttgg gaatagagca tacttgcaag cttatgtctt tattaacagc
     6961 gatatctctt attaaccaag ctatccccgt agttctaagg tttttacttt tatctaagct
     7021 aatgatcgta ttcaaaggaa atttttgacc cttagaaagt ctcattttat cagacatgag
     7081 cttaggcatt gctgaatttt gttaattcgg ttttaggttg tgctttgagc ataatatgaa
     7141 agtagtcttt atcacgattt cactcaacta aggtaatgtg atgtgattct gatattcttt
     7201 caaagatagt tttttgcata agctaacatt acatcatcaa tgagatgttt acaatgtttc
     7261 actactaaaa taaggtggta acaaagaaga aataatgaat gattatttgt atctaattcc
     7321 atggtatatc aaccatttct tatatagact gattatgaca tggattaaaa taaaaaggca
     7381 ctatgtctta attgtcggtt ttcgagttac cgaacggcat tcatcaccca cctatagagg
     7441 atgggcgact tctgcctgaa ataagttaaa ctacttataa cttctcataa atttagattg
     7501 agtcattatt atttaacatc aatgattttc cagtttccat ttttttgtaa tattaatttg
     7561 tactgaaagt agctagtggt ttttgataca ttatcaaggt attttatctt tgtactcact
     7621 agaatttttt ttccgttttg taggtaaact ggatcctcta acttcgaaaa ctttaagtca
     7681 atattgattg gtcgcatcgc atcattttca acataatact ccaattcttt ttcagaagct
     7741 gatggatata atttaaagaa cgtttccaaa aactcagcga tttcttctgt agttttggca
     7801 tcaattttcg aatcattttc tacttgtttt gtcttgtatt ttgccctatc aggttttgca
     7861 atcatagttg gcaaactcgt tactatataa ttcctgtttt catcttcaaa gatcgtcact
     7921 cggtatgcag aagaaatcga ttgtgccttt tttccctctg taatcttttg ctccactaaa
     7981 aatgaaacga caaattcctc cgagctcttt tcaacatcta agacttttac tgactgaact
     8041 tcggaattag tgggaatatc tgctcgaacc atatcttgac ttaaagcgaa accttcttct
     8101 gtaaggtact gttcgaggtt gttcattctc ttttcaattg cttctttgtt atttttccac
     8161 gaaaaatatt ctttcgaaaa atccttcgta aagttttcaa taccagaagt attcactact
     8221 ttctctttaa taaccacttt ttcatgagtt gtatgttgat caatggcggt gaaattttta
     8281 taaacgccaa aagtaagact acaagataat aaaagccaaa gggcaagcac catttttcga
     8341 tgcttaccca cggatacgac tcgttctttt ttctttttta ggctcttttc cttttgattc
     8401 cgttcaattt ttatcttcat tctttttcct cttttctctt atttgtaccg tccagcaccg
     8461 ataagatgct gctgccaata gctttctgtt agattcgtcc agcctactgg atctcctgca
     8521 tgaaacatgc gattctcccc tgcataaata ccgacatggg tcacataagt accggcatca
     8581 taagtcgaat gaaagaaaac caaatcccct ggtttggcat cttttaggtc aatatgggtc
     8641 atggcatcat actgatcttg agccacccga ggaagtttca gaccaacttt ggcataacac
     8701 cattgagtca agccactaca atcaaatcct gtttcgggtc ttgacccacc aaatacataa
     8761 gccgttcctt catacttaaa cgcctcgtca aaaatccctt ggactgtttt atcgtcaaac
     8821 tgtgttgtag ttaggtattg cttcaccaat aagcaataaa acatattgcc atagttatag
     8881 cgccatccac cattttcctt attcgcgatg ggattagggt aatcgatttt ttgaccgcaa
     8941 gatttttcct ttgaaaaaga ttcagctaaa gcaaaactat gcttcttccc gttttttgcc
     9001 acatagtcca aataaccacc gccaaagtta taggcctgaa tgatactatc ttgatcacaa
     9061 cctaattctt tggaacgctt aaccaaagac gcaaaataga gacatccttg tttgatggat
     9121 gcctcggtac ttaatgaatt cggtggtaaa ccggctgact cagaactttg catcacatct
     9181 tccattgtgc caccagattc cacttgaata atcgctaaaa gaatcggaac atactctgtt
     9241 atatccgatt ctttggcata tttttccacc attgcacggt gtttcaaaac ctcttctgag
     9301 acactactcc ccccaggagg agcacctgaa ctactacttc cttcactgtc ttcaccaaac
     9361 aataagccca cacacatcaa gatacccata aaagagattg ttaggaaagt taacgaaatg
     9421 gcacttaatt tctttacgtt cattttttct cacctgacct tggtttggga tgagggttca
     9481 aacttttttg tacttttcga ttggttcgtt tggacagagt agaccgccgt ttcacttgtt
     9541 gaaaagaatt tttcttactt gaggattggt ttattccttt tgaaatgatt tcttggctag
     9601 cgaattttcg tttgatttcg ggattcgaat ttgctctcac aatccgcttt ggtgctctat
     9661 ccttttgaga ttggaccttt gcttttatcg gaacattttc ccttcttttc ccatgaggat
     9721 tccctatttc gttcaatact ttgcgctttt cagctatttt ttcgtctcta cgttgttgtt
     9781 gtttttctcg caactctcct cgatttgctt tttcttggac cagtcctcgc ttaaactctt
     9841 ccggtgcttt tttcgttttt tcaattcctt tatggaagtt gtatttcaag ttggttggcg
     9901 tattccgtac tccatccttt acttgtttgg tcttatcttt caccaaagct tgggtatcca
     9961 gaaccttgcc gacttttctc ccggccaaat tcattcggga ttgtttctta ttatttggaa
    10021 ttggcttttc cttattcaag tcagatggta tctcgtgatc gttcggtaat cgtgaattct
    10081 ttcgtagaga agaaccggat ggcttaaatt tggattttga ttgttccttt gccaacttat
    10141 ttccaactgc tgcaccacca ccaaaagcca aagtccgtcc cacatttcga ttgagttgcc
    10201 gcaatgttcg attcattaac atttgcggtt ttcgcatgac ccgtcttccc attgattggc
    10261 tatcattact ctggagattg aacatattca taatgtcgcc caatttgaaa tagataccgg
    10321 caaaggtcac aatttgcaga aaagcaacca tgaaaaatgg ataggttgcc gaaatattga
    10381 aaaacatcgt ggagatgcta aatgcagtcg taatcaccaa agtaactcct gctcgcatca
    10441 taatcgtatt gaacaagcga ataatcgcct tcttgcccaa actctcataa gtcggcaaca
    10501 tggacaataa gaaactaatc ggcaaaaaca tcgcaaagat aataaagaga atttgcgaga
    10561 acaacataat ccctgttagg agaaagacaa agaaggatat gccaatattg aacaacacca
    10621 aaaagaccac ggtccctaag cggttcatgg tcttggtaat actgagattc gcattgtcat
    10681 tatcttcaat ctccgctttg accgcttctt cccgatcctt gcctttattt tcatctggac
    10741 ttacagacag gattttgtta acacgatctt cgccaacttc ttctatattg gaatcatcaa
    10801 attgcaagag taaccacggt tgttggactt gaatggaaaa caagctatct cggattaaat
    10861 ccacactgtc ctttccttgg ctttctgaat tcggcacgac aatctcggtt ccaaggctaa
    10921 gagccgcttc gctgacatct gagctaaaat cattgatttt tgtgatatag gtgggcgcat
    10981 acgcaatgaa cgaacccgaa agtagaaaaa tcatcacaaa attaatgaca gcccggactg
    11041 ctttggtagt ttctcgtttt aataatcctg tatacgccac ataaactcct agagctaaaa
    11101 tcatcaataa tagaaaccca aagtaaaagc ccgaggtctg gagtccattt tcggtaatgc
    11161 cggctaaggt ttgggtattc ttcccaatac tttcagccgt atctgaaatg aaatccagct
    11221 tataggcttc ttgaaccacg tagcccgtgg catttgataa gtagagacta actgtccaaa
    11281 taaaattagt aatggcataa agtccataca ttacgctctt ccctaggcca tcaccccaat
    11341 tccaaggcag ccaatcccac gaactatcca caaagaaatc caactgatag ttaccaagtg
    11401 aatattggga ataaagattt ccggctccaa ctgtatcatc aaccagacct gttgcctcag
    11461 ctaccgttcc aactaagctc aaaagtatta tcagagtgac caaactgatt aaaacaattc
    11521 caaagatacg aaaaagtttc tttttcatct cctcactcca ttctctcttg aactggcgga
    11581 cgggtatcaa aagcatggaa aagttcttca aagactggat gaacttggac cacacctact
    11641 cgaccataca aatcctgcat caagcattgc ccattttcca aatctcgcat gcatttttga
    11701 ttactttcat cttctttatc taatccaaag aattccaagg ttttcttgat ttcatgaata
    11761 tcggtactgc gaaatgcaaa ctttagccca atattattct tcaaacgttc atccaacaag
    11821 tcatcactgt tttgcgtgac gaagtaaaca ccggcattca tggcccgacc agcacgcact
    11881 aatttattcg ataacgtttt tccttgagct acttgaagaa aactccaagc ctcatccagg
    11941 tccacaatct tgaaaatttc tcggttgcta tggataaagt ccaaggcaaa agtggaaata
    12001 acaattagca tggaaacact cagcaattcc atggtggtat attcttcaaa agtggtttct
    12061 gcatctggta aaaccaaatc cgctacttga atgatattta actgcttatc taaactaatc
    12121 gactgggtaa tcgaaccatc tgagaaaagc aaatgggcaa aatcataatc ggtaaaggac
    12181 tcgatgtgat ccgcgatatt ttccgcgact ggtgagtcct ctttacgcaa ttcaacgatt
    12241 acttgtaata atcctcgctg atcactttgg gtaaccgttc gaatggcttt gcgtaacaca
    12301 gggaacttct ccccatcccg actggaaatc cctgttaaaa aggtcaggat atcaatcgcc
    12361 aaactttctg agtctttctt tttcttcata atcacatagg gatcaagtaa gccttgattt
    12421 tctggcttac tggtcaagtt aagaatatta atttcatccg caatttctgg caacgtttct
    12481 ttccaaccgc ctctttctcc ttttggatcg acaatcacag cttgcccacc aaaaaggacg
    12541 gcataataga ctaagagatt gttgctaaaa gatttccccc cacctaacga accaaggaaa
    12601 gcagatgcca aagcattggt tactgagcct ttgacccctt gtgatgccaa tgccggtttc
    12661 aggtaaacat tgcgccccgt atctacgtta tagccaaagt aaatgccttc taattctcct
    12721 agcatttgag tagccccaaa accaagtccc gccaaaaagt cagacgtaac atattgaata
    12781 tagtcattca tgtatcgctt acttgagggt aagaattctt tgtgtagacc catcatatcg
    12841 ccaaagggcc ggactagttt tacattggta tcgtcataaa aatcgagcac ttcatcacag
    12901 cggcgtttta attcatccac tgatttagca ctcactcgaa ccacatagct tagtttatac
    12961 atggattctt tcgactgatc caaggtggtt tccaattcat ccacggaatc caaagcatct
    13021 aatacattgg agttcgtttc attatcagat tgataggcat ggttgtctaa atcttttaat
    13081 tcctttttct tattacgaac ggtagctagg gcctttttat tcgtcacaat ttccacattc
    13141 attgaagtat caatcggaaa cgtaaattgt tgttgctggt aatagaaaag ttccgaggaa
    13201 ggaaactcca tttcccctac aatggtatta attgttaagt acgctacata agactcatga
    13261 ttttcatgtt ccatccgtaa gtaccgaggc ttttcttcaa tcaaacaacg agtgggacgt
    13321 aataagtcgt aacgtttcac cagcgtttct gatttcagct ttttctttgg tagctggaat
    13381 tcatattctt caaacggtgt gcctttttca ccatagatat gttcaattaa atacgtcaag
    13441 tcactcggcg ttactcgttt gactttaaat cttcgagcca atttactttc cattagtttt
    13501 tcaagcttgg tatagcgacg aatttcttca ttggataagg aaacaaaatc acccatcaaa
    13561 tgatgattca ctccatagac aaattcttga agtccagaaa agaaagattt cctgagattc
    13621 tttagattga cctcttcatc tgtcgaaatc agtttaaagc caataaagaa gcgataatca
    13681 atctgactat cgccaatcat ggatactaat gcatctgtct gtaaatcaat tcgttgcttt
    13741 gctacttctt tgagtctacc ggtaatttcc tttttcgaac gttcttgtgt ggcacgaata
    13801 ctactttctg tggcaatttg taaggcatgg atcttacctt cacgattttg cgcaattaat
    13861 tggcgaaagt tatcatgtac ttgatacttc tgttcaggtg acaaaaagga gtaattgtaa
    13921 ggcactaatt catagtaagc aaaacactcg ccttcttggt taaagacaag attattttca
    13981 atatacttaa tcgggtacat aattcacact ccttaccaag gtgattg
//
LOCUS       DACDCJ010000073        13809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDCJ010000073 DACDCJ010000000
VERSION     DACDCJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.073"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IV038_002200"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IV038_002200"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP8342390.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="IV038_002201"
     CDS             complement(2262..2513)
                     /locus_tag="IV038_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP8342391.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="IV038_002202"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="IV038_002202"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP8342392.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="IV038_002203"
     CDS             3463..3681
                     /locus_tag="IV038_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342393.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="IV038_002204"
     CDS             complement(3970..4482)
                     /locus_tag="IV038_002204"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP8342394.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IV038_002205"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IV038_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP8342395.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7369)
                     /locus_tag="IV038_002206"
     CDS             complement(6935..7369)
                     /locus_tag="IV038_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP8342396.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="IV038_002207"
     CDS             complement(7379..8167)
                     /locus_tag="IV038_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8342397.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="IV038_002208"
     CDS             complement(8164..9105)
                     /locus_tag="IV038_002208"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP8342398.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="IV038_002209"
     CDS             complement(9282..9869)
                     /locus_tag="IV038_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP8342399.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IV038_002210"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IV038_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP8342400.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="IV038_002211"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="IV038_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP8342401.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="IV038_002212"
     CDS             complement(12786..13721)
                     /locus_tag="IV038_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8342402.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3806 a         3096 c         2435 g         4472 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa tatcatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataac
//
LOCUS       DACDCJ010000074        13678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDCJ010000074 DACDCJ010000000
VERSION     DACDCJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.074"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IV038_002213"
     CDS             complement(<1..902)
                     /locus_tag="IV038_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP8342403.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IV038_002214"
     CDS             1185..2153
                     /locus_tag="IV038_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8342404.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IV038_002215"
     CDS             2266..2544
                     /locus_tag="IV038_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP8342405.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IV038_002216"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IV038_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP8342406.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IV038_002217"
     CDS             3010..3756
                     /locus_tag="IV038_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8342407.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IV038_002218"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IV038_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP8342408.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IV038_002219"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IV038_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP8342409.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IV038_002220"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IV038_002220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP8342410.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IV038_002221"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IV038_002221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP8342411.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IV038_002222"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IV038_002222"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP8342412.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IV038_002223"
     CDS             8493..9035
                     /locus_tag="IV038_002223"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP8342413.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IV038_002224"
     CDS             9058..10614
                     /locus_tag="IV038_002224"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP8342414.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IV038_002225"
     CDS             10628..11530
                     /locus_tag="IV038_002225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP8342415.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IV038_002226"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IV038_002226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP8342416.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IV038_002227"
     CDS             12973..13395
                     /locus_tag="IV038_002227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP8342417.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4521 a         2448 c         2980 g         3729 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca
     9901 ggtcgtgaag cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgcctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaagta attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgctgctc agccgtgctg tgaagaaaac tggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattacaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggggaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca
    13561 gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatt
//
LOCUS       DACDCJ010000075        13625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDCJ010000075 DACDCJ010000000
VERSION     DACDCJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.075"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..307)
                     /locus_tag="IV038_002228"
     CDS             complement(<1..307)
                     /locus_tag="IV038_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8342418.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     "
     gene            complement(307..537)
                     /locus_tag="IV038_002229"
     CDS             complement(307..537)
                     /locus_tag="IV038_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8342419.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            824..1162
                     /locus_tag="IV038_002230"
     CDS             824..1162
                     /locus_tag="IV038_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342420.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1367..1606)
                     /locus_tag="IV038_002231"
     CDS             complement(1367..1606)
                     /locus_tag="IV038_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP8342421.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1582..1929)
                     /locus_tag="IV038_002232"
     CDS             complement(1582..1929)
                     /locus_tag="IV038_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP8342422.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2661..3242)
                     /locus_tag="IV038_002233"
     CDS             complement(2661..3242)
                     /locus_tag="IV038_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP8342423.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3425..4051)
                     /locus_tag="IV038_002234"
     CDS             complement(3425..4051)
                     /locus_tag="IV038_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8342424.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4073..5404)
                     /locus_tag="IV038_002235"
     CDS             complement(4073..5404)
                     /locus_tag="IV038_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP8342425.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5559..5960)
                     /locus_tag="IV038_002236"
     CDS             complement(5559..5960)
                     /locus_tag="IV038_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP8342426.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6046..6252)
                     /locus_tag="IV038_002237"
     CDS             complement(6046..6252)
                     /locus_tag="IV038_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342427.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6347..7849)
                     /locus_tag="IV038_002238"
     CDS             complement(6347..7849)
                     /locus_tag="IV038_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP8342428.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7867..8322)
                     /gene="argR"
                     /locus_tag="IV038_002239"
     CDS             complement(7867..8322)
                     /gene="argR"
                     /locus_tag="IV038_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP8342429.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8420..9037)
                     /locus_tag="IV038_002240"
     CDS             complement(8420..9037)
                     /locus_tag="IV038_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342430.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9258..10631
                     /locus_tag="IV038_002241"
     CDS             9258..10631
                     /locus_tag="IV038_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8342431.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10706..11818
                     /locus_tag="IV038_002242"
     CDS             10706..11818
                     /locus_tag="IV038_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP8342432.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11890..13527)
                     /locus_tag="IV038_002243"
     CDS             complement(11890..13527)
                     /locus_tag="IV038_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP8342433.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         3708 a         2869 c         2399 g         4649 t
ORIGIN      
        1 ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta aaatcaagag
       61 attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt aatgagtaac
      121 gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac attgttttac
      181 ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt tcttttccta
      241 cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa attctttctt
      301 ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt ctttctcata
      361 atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta acgtaattgt
      421 tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct ttccgttatc
      481 agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa caagcattat
      541 catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat tcccctttgt
      601 aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa tagaaataaa
      661 ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat tgaacctatg
      721 gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt ttgcagataa
      781 aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg attataaaca
      841 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
      901 aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag aaacattggt
      961 tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg tagctatttt
     1021 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
     1081 tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga attttagatt
     1141 ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa aagactcgca
     1201 gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga ccctatagat
     1261 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
     1321 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
     1381 ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata tctgttaccc
     1441 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
     1501 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
     1561 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
     1621 cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat gaaaggtctt
     1681 tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt taaatccggt
     1741 aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc ttaacgtcac
     1801 aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct tcggttgttt
     1861 cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa aaaaataacg
     1921 accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac aagaagttcg
     1981 caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag tgacaaactc
     2041 tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca
     2101 tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca ggcttacgac
     2161 ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     2221 tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga ctttttcaca
     2281 acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
     2341 aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg gcagcttttt
     2401 cacaacctct ccactaaacg atgttcaatc agctaacctt cggtattagc tcaaaaaacg
     2461 gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca aagcgctgat
     2521 ttcacaacct ctaatacacg gtgttccaga agtagcttct tcgaaaaata agccgaaatt
     2581 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
     2641 tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag ttaacaatct ttctttgatc
     2701 aattgaaaat tctgcctaag tagtgataga tcctgtgtga ctttcaacaa taagatactt
     2761 ccctggattt cagcaatcaa cgctttggct tgacggtctg cttcttcatc ggacagtttt
     2821 tcttcagaaa gtgccaggat attttgtttc gtatacttga cccattgatt gactaattct
     2881 agtagcaaca gacggtattc ttcatccact gtactcaact caataatcaa attacctaca
     2941 ggacagccat gataagtgat ctgtgtctca tgatcagcga aaatccagtc caacatttct
     3001 tctaatgctt cttctggctg ttttccagca tttaagatgt cttcgaacaa ttctgtctgc
     3061 catacgtcca tatgcgcatg gatcaccgct agacaaactt ctttttttga agaaaagtaa
     3121 taataaaatt gaccttttcc tgtatcagaa gctgctagaa tatctcggat tgacgtttca
     3181 tgatagcctt ttttataaat aagatctttg gtcgcttcca agatcttttc ctttgttttc
     3241 aatgatattc tcctttttca tttctcatag tcctatatta tacagtaaat tcatcatttt
     3301 ttcttcttaa tcgtttaaaa aagactgtgc caaattatct gacacagccc tcactataaa
     3361 actgttctca tatagctaga tgttcattga ttcaagaact gcgaggacaa aagcaattcc
     3421 tatcttatga tagttgctct aatacttcct ctgttgtcat cacttgatct gcaataaagc
     3481 caaagttcgt caacgctgca tccattgctt cttggccagg agcaccaatt gcatctgtcg
     3541 ctaccacaac attgaatcca ctctctacca attcacgcat atgggaatcg ctacacaagt
     3601 ttccgttcat tccaccaaga attactgtgt cgattccatt ttttctcaat tggagcgcca
     3661 aatcattgct ttctggtcca aagattttat gaggagagct gatgatcgta ttttcatcca
     3721 aataaggttt caattcttcg ataaaatcgg ctcctgaacc ttcttccact gattcatatt
     3781 gactttttct agcaaacatc tgctgctcaa gcatcatatg ttctcctgca ccgccaaatt
     3841 gccatttatg gtcatgtggg taaaaataat gaggagaaat aaagagtttg tatccttttt
     3901 cttttgcttg ttttaccatt tcaatcatat tttcgatcgt attgtatttt tctaagatat
     3961 cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc gggtcagtga
     4021 gaactactgc tgtattttta ttattgaaca tggtggattc tccattcgtt atttatttta
     4081 atacatcatt tagagcttca ctcattgtcg gatgagaata gatttgatct ctcaataacg
     4141 tataaggaag ttttccattc atagctagcg aaatcaagtt gatgacttca tgagaatctt
     4201 ccgcatagat acttgcccct agaatcaagt cagtttcagg gtcgactaaa actttgaata
     4261 cacctttagg atcttctaat acttgtgctt tagggacacc gcttgtcata tacttgaata
     4321 atttgtaatt gacgccttgt gctttggctt gtttttctgt taaacctaca ttggataaag
     4381 tcggagtgat aaatacactg taaggcacag ctttccggtt attcgttgtt cgtttattct
     4441 cgcccttcaa ctgatccata atgattcgat aatcatctag agagatataa gtgaactgca
     4501 atccgccttt gacgtctcca atcgcccaaa tgttgtctgc atctgtacga aggtattcat
     4561 ccacagcgat tgctccgcga tctgttagtt taacatctgt attttctaat ccaagtgatg
     4621 ctgtacttgg ttttcttcca gttgcagcca aaacttttga cgcttggatt gttacttctt
     4681 ggccatcttc tgtaaaggtg acggcagctg ttgattcttg gtctgctact tgatctacag
     4741 atacacctag atggaattcg attcccgcat ttgtcatgtc ttctaaaatc atttgcgcga
     4801 tatcttcatc ttcacgtgga agaaattcag aatgggcatc aagaacgaca actttagatc
     4861 cgtattcatt gaacattgat gcaaattcca agccaatata tccagccccg atgatcacta
     4921 atttttccgg taattgttcc tgatccatcg cttgcgtaga atcaatcaag tagcgacttg
     4981 tattcaatcc tttgattggt aaaatgattg gttcagaacc agtattgatg aaaatccgtt
     5041 cgccttcaac aatcactttt tcaccattat tcgtcacttc gatttgatgg ttagaaagaa
     5101 aacgtgctgt accgtccatc actgtcccag ttgcctcatc gctgatcata tgataatttt
     5161 tggcgcgaag catgcccgtc aatttttctt tccgagcaac agcctctgta aagtcgattc
     5221 ctctttctcc attgaaaatc aatgatttag aaggaataca gccgatgttg atacatgttc
     5281 cgccatacat acgtgttgac ttttcaacga ctaaaaccga ttcacctttt gaagctaacg
     5341 ccttcgctaa agtcttaccg ccctttccaa aaccgatcac aatattttga tatttttcca
     5401 tcatttttcc ctcgcttctt ttttacctac cgattggtac aaaaatatga taaccgataa
     5461 ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa aaagcggtcc
     5521 tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt tacttctaca
     5581 tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc ttctgcaatt
     5641 ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac accgatctga
     5701 tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga ctctgctgtc
     5761 attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc ataacctgct
     5821 gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt gattttcata
     5881 tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt gatcattgct
     5941 gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct acttggctat
     6001 aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt cgaataccag
     6061 aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga acaacagcgt
     6121 catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat gattgatttt
     6181 aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa aaataaccat
     6241 attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga tttaaccata
     6301 ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat tctgctgtta
     6361 atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac gccaacttgt
     6421 tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt aagcttaaac
     6481 ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata ccttcttcat
     6541 tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt tcttctaata
     6601 tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt tcttgtactt
     6661 ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt ctttttaatt
     6721 ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga aaatagtttt
     6781 ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc ccaagacggt
     6841 taagttctaa tgctttattg attacatttc gatagccgga aatataacga acttggatgc
     6901 ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc gtcacgattc
     6961 ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa ttatttcgat
     7021 tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta tcgaaaatga
     7081 tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc gcttctttat
     7141 tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat tgggaacctg
     7201 ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca gcaccatttc
     7261 cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt ccaatcccct
     7321 ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct ttttctacgc
     7381 ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca atatcttctt
     7441 tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag ggaagatggc
     7501 tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg gacataaaca
     7561 tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct ttcagacttt
     7621 ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca atcccaatcg
     7681 tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt gctagtcccc
     7741 agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca actggtaatg
     7801 cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata taaaagtcct
     7861 cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt cttcttctga
     7921 gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt cagggtaagc
     7981 cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca atacgataac
     8041 catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac gcaaacgttc
     8101 ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt tgttttcatt
     8161 ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt gagtcacatc
     8221 ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag gttgttctgt
     8281 aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca cctctcaatg
     8341 ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt acttttattt
     8401 tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc gtcttcattt
     8461 tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct ttagaagctc
     8521 tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct ggttcttcgg
     8581 catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt ttgaatacta
     8641 cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct tgtgctatcg
     8701 cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt tcttttttca
     8761 tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt ggatagacag
     8821 tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt attgcatagc
     8881 tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg tcgccgatcc
     8941 ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt tttttcatac
     9001 tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg cgaaattcca
     9061 tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt tgacactatt
     9121 tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac gtttcggcgt
     9181 gtcgagagag aggcggtttc tatttttata atagaaatca ctctcttttt ttacgaaatt
     9241 ttttagaggt gatgtaaatg acaaaacaaa aacagctgct tttagtacct gcagtaattg
     9301 caacagggat gatgtctttt gcgggcgtat tgatcgagac ggccatgaat gtaactttcc
     9361 caactcttat cagacagttt gatttaacga ctgcacaagt ccaatgggta acaacgattt
     9421 atcttttgat gatttcgatc attgttccgc tgtctactta tttgaatcgc aattttagtc
     9481 tcaaaaaact ctttttagct tcaaatctgc ttttcttggc aggtataatg atcgatttct
     9541 tctctcctac cttcatggtc ctattgtttg gtcgattgct ccaaggtgca gcgacaggta
     9601 tcgctcttcc gttgatgttc catattatct tgacatttgc ccccctcaat cgacgaggcg
     9661 cgatgatggg tgtcggtact ttgacaacgg ccattgctcc agcaattggc cccacttatg
     9721 gaggcattat gacttcacat tttacatgga atcatatctt cctgtttctt gtgcccgttt
     9781 tgattctttc tttgattatc ggctggtttg ctattcccga gatggcagta caaaaaagtg
     9841 gttatttgga ttggataagc gttttaggaa tttgtctttt gtttagtgga agtctaacat
     9901 ttttgagtac gttgggaaca gtttccagct ggattgcttt aatcatcggc ggtattggct
     9961 ggttcctttt ttactatcga agtaaaaaag tggagcatcc actgatccga ctgtctattc
    10021 tttcaaataa aacattccgc ttgtttttga ttggcttctt agtttatcag tttttattac
    10081 ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt agtccttttg
    10141 tcgctggatt agcgatgctt cccggcgcag cggttggtgc tttgctttct cctttttccg
    10201 ggaaaatgct ggatcaatat ggtccgaaga agccaatttt tgctgggctg attctttctt
    10261 tgattggttg gctagcatta ttcctactca taggacacgc ttcagtgggt gtattgattg
    10321 cttgtcatgt gttttatatg atcggtatcg gattatctta cagcaatatg atgaccaccg
    10381 gaatgaatgc actgaatgaa gaactacaag gagacgggaa cgcaatgttc aataccttgc
    10441 agcagttttc tggcgctgtg gctacttcat tagtagcggt cattatcaat tacgtccagc
    10501 atcatacttc ccataactat gaagtgtcta ctacattagg atcaaaagca gcattaggcg
    10561 tcttgttgct gttgcttctc gtaagctttg ctcgattcgc ctattatctg ttctttgcta
    10621 aaaaagcgta gattccatat acggcaggtg ttgcatctgc cgtttttttg tgtgaaaatg
    10681 gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg gcgggatcat
    10741 tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt tatttgatga
    10801 agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt tgtcgaaacg
    10861 cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc ctaaacttat
    10921 aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt tgcttcgacc
    10981 tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag aagcgccaga
    11041 aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc cgttattgaa
    11101 accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag catatttaga
    11161 acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa aagtcacttt
    11221 gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca cagctgatcg
    11281 tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag gctatgctgc
    11341 agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata agatgagtca
    11401 agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca tggatggaaa
    11461 gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc ctactagcga
    11521 agcttataag ggattgacta agagtacaga acagttcttg actgatccag atcagataaa
    11581 aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt tttccccatt
    11641 tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag gctcttctgg
    11701 cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata ctggtcattg
    11761 gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc catcatagaa
    11821 aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact tcttgaaaaa
    11881 gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg cttcatctgg
    11941 caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa gtcgttgttt
    12001 caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta taggtaaata
    12061 gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca cgattctttc
    12121 aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc cagtattcag
    12181 gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat agtcttccct
    12241 aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat ctagctgtct
    12301 ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac caaaagacgc
    12361 tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt tcatgatcca
    12421 aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg cataacgtga
    12481 tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt tttctccttt
    12541 acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg ccggatcatc
    12601 agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag caaaaccatt
    12661 ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac cttccgtatg
    12721 ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag ccatcgtata
    12781 ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa agccattgcg
    12841 atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa agtaacgtaa
    12901 tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat ttggaaatgt
    12961 ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa aggaaacaga
    13021 cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca aataagaata
    13081 caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa attcttcaga
    13141 taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat acacagcttc
    13201 tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga tccgccgagc
    13261 acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa catcagctgg
    13321 taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc ctggactccc
    13381 ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat taccgtaaaa
    13441 tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg tgatttcgtt
    13501 taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca aaaaaacgtt
    13561 ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg aagcaaacga
    13621 gttat
//
LOCUS       DACDCJ010000076        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDCJ010000076 DACDCJ010000000
VERSION     DACDCJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.076"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(78..150)
                     /locus_tag="IV038_002244"
     tRNA            complement(78..150)
                     /locus_tag="IV038_002244"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="IV038_002245"
     CDS             531..1100
                     /locus_tag="IV038_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP8342434.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="IV038_002246"
     CDS             1087..1947
                     /locus_tag="IV038_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8342435.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="IV038_002247"
     CDS             2137..2841
                     /locus_tag="IV038_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8342436.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="IV038_002248"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="IV038_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP8342437.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="IV038_002249"
     CDS             4678..5994
                     /locus_tag="IV038_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342438.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="IV038_002250"
     CDS             5995..6864
                     /locus_tag="IV038_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342439.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="IV038_002251"
     CDS             6919..7728
                     /locus_tag="IV038_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP8342440.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="IV038_002252"
     CDS             complement(7831..8460)
                     /locus_tag="IV038_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP8342441.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="IV038_002253"
     CDS             8630..9922
                     /locus_tag="IV038_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8342442.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="IV038_002254"
     CDS             complement(9998..10660)
                     /locus_tag="IV038_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP8342443.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /locus_tag="IV038_002255"
     CDS             10885..11169
                     /locus_tag="IV038_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP8342444.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="IV038_002256"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="IV038_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP8342445.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2264 c         2833 g         3679 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaaata
    13141 tccatt
//
LOCUS       DACDCJ010000077        13090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDCJ010000077 DACDCJ010000000
VERSION     DACDCJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.077"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(286..900)
                     /locus_tag="IV038_002257"
     CDS             complement(286..900)
                     /locus_tag="IV038_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8342446.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1506..1883)
                     /locus_tag="IV038_002258"
     CDS             complement(1506..1883)
                     /locus_tag="IV038_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342447.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2183..3064)
                     /gene="galU"
                     /locus_tag="IV038_002259"
     CDS             complement(2183..3064)
                     /gene="galU"
                     /locus_tag="IV038_002259"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP8342448.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3107..4126)
                     /locus_tag="IV038_002260"
     CDS             complement(3107..4126)
                     /locus_tag="IV038_002260"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP8342449.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4759..5763)
                     /locus_tag="IV038_002261"
     CDS             complement(4759..5763)
                     /locus_tag="IV038_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8342450.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5776..6825)
                     /locus_tag="IV038_002262"
     CDS             complement(5776..6825)
                     /locus_tag="IV038_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8342451.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6842..7663)
                     /locus_tag="IV038_002263"
     CDS             complement(6842..7663)
                     /locus_tag="IV038_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8342452.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7650..8429)
                     /locus_tag="IV038_002264"
     CDS             complement(7650..8429)
                     /locus_tag="IV038_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8342453.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8446..8916)
                     /locus_tag="IV038_002265"
     CDS             complement(8446..8916)
                     /locus_tag="IV038_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8342454.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8928..9347)
                     /locus_tag="IV038_002266"
     CDS             complement(8928..9347)
                     /locus_tag="IV038_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP8342455.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9428..12196)
                     /locus_tag="IV038_002267"
     CDS             complement(9428..12196)
                     /locus_tag="IV038_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8342456.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            12396..12620
                     /locus_tag="IV038_002268"
     CDS             12396..12620
                     /locus_tag="IV038_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342457.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3837 a         2740 c         2124 g         4389 t
ORIGIN      
        1 agaaagaatc catatggatt ctttcttgtc tctttttcca ctacttttta tttttctgac
       61 actacatatt cttataactc atttctcata actgtgaaac aacttataaa aataacaaac
      121 tttaaacaaa acaagcttaa aatcaaagat gatccatctt aaaataacta aaacaaaaaa
      181 agtagtcgtt gttaatcctc attaataatc gttttctttt tttcaaacgc tccattttta
      241 tgtggaacat tattttttta aggaactttg tcaacatgtg gataactatt ttccacttgt
      301 gtcctggact agctgttttg ctgtaaaata caaaacttgc tgcagcaaaa agatcgttgc
      361 aagagaggct gttccgcaga tcgctggatc tagatttggc aaagtcaaga ctgcatcatg
      421 taaattggcg tggttgtcca aaaccaaatc agcaaattca aataatcgca aaccactgct
      481 atgttgcgat ttgatacttc gtgacaaatc aaatccagta acaatgatta atggatgacc
      541 attttctttg atccactgag ctagttcgat gattgccggg tttcttcctt cattcgaaag
      601 aagaagaaaa atgtcttctg atttgacaga aagctgatca ataattgcct gtcctgcacc
      661 ttctagcgtt tcatagacac ctccggctgg attttcaatc aatatacttg gtacaagatc
      721 cggggtttga tcccaaaatt caaatgctac accacttagt gtgcggctag catatatctg
      781 caatctacca ccttgattaa tttgtttcgc gatccaagtg ccagcttgaa gcatcagcgg
      841 ctcttctatc cttaatttct cttccaattg gtgataatag tcaaaataac ataaactcat
      901 ttatctctcc cgcctagcta tacttgacat gaatagaaac agctgcctaa tctaaggaag
      961 tacataaaaa aacagtagtt agacagtaac acatgtggtc tttgctgttt caaacacaaa
     1021 aaaggcattg aacgaatacg cccaatacct tgacatagca aataaaacaa cgtgcagacc
     1081 atggaaaata tctacatagg ggtagaattt tcaccctaga ttaggtacaa taaattaggt
     1141 cgatgcactg cattgactat ggcaaaatga tcggtcaggg tgtatgtgtg tgactggtca
     1201 ttccctgatt cttagcgcca actaagggtc agggagcctt tttttattca gctgtttcta
     1261 tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc aagaaaatac aaaaaaacat
     1321 tggtatgatt tatatatata taaataacat aaaaaaagga atggcttatc ccgatacaac
     1381 aagcattcct ttaaaaaaga ttcttttata taagtgaaaa aagctttttt ccaccaatta
     1441 aataaatctc tcatctcaat agtttatcca ttattttatc tttttttgcg tatacctact
     1501 aaatattacc gtcttcttct cgttcttcta ctttgttcga gagaatctgt actagaaaaa
     1561 gtagttgtat gtctaccttt tctgcctatc cgagactttc ttttcatata tgggatataa
     1621 atgattagat acagtacagc aaaaaataaa gcaaatacgc aagcttccca actacctaac
     1681 gccaattcaa ccatcaaggt agcaacccca acggatgctg cataccagat aaaaatcaga
     1741 aacgtttctt tttttctgac catgcgaaac atgataaata gactaataag caatagtggc
     1801 aacacaaata tccaattgct tccttccatc agccacttga gtatatcaag cagcgactgt
     1861 gcaacagcga ctaattttcc catagtgaac acacaccttt ttttataatt aaaaaattcg
     1921 cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg taatcatttt
     1981 cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga atctattcac
     2041 gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc aaaaaactaa
     2101 aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag cctttcattc
     2161 tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc aactggataa
     2221 tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca atatttgtct
     2281 tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct ctcgcataga
     2341 ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg tttcctgctc
     2401 ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa cgtccgataa
     2461 tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct tttactctgc
     2521 aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat gtttcatttt
     2581 ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc atcatcaatt
     2641 gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg acaaacggtt
     2701 catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt ggatgagatt
     2761 gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt aattctgttt
     2821 tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga tcttcaatgg
     2881 gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc aatgcttctt
     2941 ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt gccatggctt
     3001 ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct tttttgacct
     3061 tcacttgctg ttcctcctaa acgacgatta actattgatc atctatttaa cgaagagaaa
     3121 attcattctc catttttccg tcacgtaaca tgatttcttt tgctgctttt ttgatatctt
     3181 ctccattata caaaacatta tagattgttt gagtaattgg catctcaaca cctagctgtt
     3241 gagctagctc tactgctgct tttgtcgttg ccacaccctc aacgaccatt cccatgtttt
     3301 ctagaacttc ttccagctta tgtccctgtc ctaacaagtt accggcacgc caattgcgag
     3361 aatgaacact tgtacaagta acgatcaagt cccccacacc actcaaaccg ataaatgtca
     3421 gaggattggc acccatcgcg acaccaagac ggctgatttc tgccaatcct cgagtcatga
     3481 ttgctgcttt tgcatcatct ccgaatccta atccatggat agcgccggca ccaattgcaa
     3541 tgatattttt cagtgctgca ccagtttcta cgccaatcac atcattattt gtataaatcc
     3601 tgaaataatc attcatgaac agtttttgta cataggctgc tgctttttga tcaatacttg
     3661 cagctgtaat tgttgtgata tcatgtactg ccacttcctc agcatggcta ggaccagata
     3721 atacaacaat tgcctgtctt ttttcttctg gaatttcttc agccaaaact tctgagattc
     3781 gtttatgcgt atcttgttct agtcctttac ttgcatgtat aatgattggc ttagtattca
     3841 ttttaccaat aagttcctgt gcgactgaac gaatcgcttt tgttggaaca acaaataaaa
     3901 tagcatctgc gttatccact gcttctgcca attctttata tccgataatc gattctggga
     3961 tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt attgatttca tcaatctgtt
     4021 caggtacatt tccccaaata cggacatcat gcccgttttc tgccaatgtt tgtgctaaag
     4081 cagtgcccca agatccagga ccaagaactg cgattttttg tttcatggat attcctccaa
     4141 ataaataatt gataatttcc attatagtat acatttctta aagttgggac taatttccat
     4201 tgtttttaag tgtttgttta cttcttttat cgtttttact tgaaataatc aatgatatgt
     4261 tacaatagtc tacattttat ttttacactc gttatgcttt gcttgttcct aagaggaagc
     4321 agttttgaca atgttactac cagcattact attaggaggt taggaaactt ctcctagtga
     4381 gtattgattt aaaatacata agagacagat tgaacatttc taaatctgtc aggtcctgca
     4441 acggacagat tgtctctgtt gacggttgac ataggaactt ttgttttagt caacattctg
     4501 gatacaaaac gctatgtaaa ctggcgcaac tcgttgtact tggactttta ctacggatcc
     4561 atgcaacaac taaatagtac tattgcataa caaaaaatgg ttggcaccca catgtcccat
     4621 taaacagatt ataagttgaa ctgtagataa tgcaaaaaat gtgttttata acgtaattga
     4681 tgccaatatt ttaaaaaaat aaagggagca gttatcgctg ctctctttat tttttatatt
     4741 ctttttatcc agtctatcct aataatcaaa ctgacgatag tagcggcgta tctctaatgg
     4801 atggcagttc aactgctcca catgagcatc gatgcgttgt gtcactacat gcatcaataa
     4861 gaaagacaaa acacgtccgc gatatttttc actgatacct tctactggat agtcttttgt
     4921 gtctatcaat gtataattgc tgcaaatttt tggcaaaagt ttagccacac gttcagataa
     4981 cactctctgt tcatcttcgc ctaaaaataa tgtgactggg gttgtttctt ctacgatctc
     5041 taacgtcccg tgaagaaact cggcagcatg gatagatttc gatggaagcc agctttgttc
     5101 ttcccagtaa cacatggcat atgaataaac agctccccat tgattccctg ctccaataaa
     5161 ataatgcata ctatcacgtc gatgtttttc tgcaaatgat aagccaaatg catctgcttt
     5221 tttctctgct tccactaacc ctgttggtaa ataacgttcg agctgttcat agtattctga
     5281 ataagcttcg aattctccat tcaaatacat caatcgatct gctgccataa aaaatttgat
     5341 ttgttctgtt ccaggagctg gataagtgac cacaaaatcg catagctgag acaacttact
     5401 gttcgcttta tcgataaatc caatcagtgt cgctcctact ttcttgattt gttctactgc
     5461 tttcactact tcttgagttg taccggtaac agaggacaaa ataacgatag aatcttttgt
     5521 taatcttttg tttccagtag tgtaatattc tgcagcatgc tgtacaaaga caggcaagtt
     5581 gcttttccca ttcatataag tgacagcttg catcgaggaa gcatacgttc caccgattcc
     5641 tagataatag acagcgtcaa atttttccgc ataaagttta tcgatgattt cttctacttg
     5701 cggacgtaaa gctagtgcgc cttttattgc tttgatttgt tcttcttcat tgaatttcag
     5761 catgatttcc acctcttata atttactttt caagactgta tcaaagtctt caaaaatctt
     5821 tttgttcaag tcaatacctt tagctactgc ggttttataa gcgaatagtt gaaacggaac
     5881 gacaagcgct aatacagaca gatattcatc acattctatt gctaatccaa gtgttttctc
     5941 gtttcttgct ttttttgtgg taatggtgag agtctctcca acgtaccgac tcatatatct
     6001 ttgcaacttt tgcgaacgtt ccttatttac actttcccct tcaataaaga atagcaaatg
     6061 cgaagcatct gcctccaggt atgggccgtg catataagct tctaactcaa atccttgtga
     6121 cggaacgcgg attgtttcag tcaatttcgt ctctgcttct ttagctgttc cccagtttgg
     6181 accatatcca atggtgacga aacgctgtgc taaacgaaat aaactttgat atccatcaaa
     6241 aaattcttcc gttttttgaa taatcgctgg taaatggttg attattttgc gcaactgttg
     6301 catgtagtct tgttctattt ttttgctgat tttattttta cttattccaa ttcctatccc
     6361 taagagtagt aactgtaaaa cagtcgcaga atagcctttt gtaacgaagc ctactgattc
     6421 taccccggca cctaaatcaa taatttgtgc agcttctaaa gcaagtggac tttgcacgtc
     6481 atttgtgata gcaacaacag gtaacccttg tttttgtatc atttttactg cttcaacagt
     6541 agaagcactt ttcccgcttt gtgacacagc aatcacagta tcgattgaag gagaaagttt
     6601 tccataattg aaaaaataaa aaggttcttg aatatcgaca gtaagatcac cataagattc
     6661 caatgctggc ttcgctgcta aacaagcgtt ataagatgaa cccgttgcta agatcagtaa
     6721 attcactgtt tttttcatat gttctcttgt cgaaaaatca ttctttttca agatcgtact
     6781 tagtacttcc ttttcttcat agatataatc ttgcatcgta aacattttct ctgctccttt
     6841 tttaaaaaat ccctatccac gaaccaaaaa ttccaaagaa agctagtccg atcaagatcg
     6901 ttaatggttt cacttctttc ttcaatagat aaaacactat gccaaatgct ccaagacata
     6961 agatatttgg aacgatatca tcaaaaatac tttgaacagt cacagcattt tctcctgaac
     7021 cgatttttaa aggaatattg atggtgatca tcgtggcagt cattgctcca acgaccatca
     7081 gtccaataat agacgcgcct aatgtcaaac tttccatcat accgtttgcc tggatctttt
     7141 tcaaaaaacc cgttcctaag cgatatcccc aacctgttgc tagatagcga aacagaatgt
     7201 gcggaatatt gaaaatcaaa atgaacaaga tagggcctaa aatatttcct tgcatggcta
     7261 aagaggtacc cacacctgtg gcaatcaatc gcaaagttcc ccaaaagaaa gaatctccta
     7321 accctgcaag aggtcccatc aaagatgttt tgatactatc gatcgcagtc acatcaaatt
     7381 caggatcatc cttattttct tcctccattg cagccgtgat tcctaaaatc agtgtaacga
     7441 tatgtggggt cgtgttaaaa aactctagat gccgtttcaa agctgctgcc atttcttctt
     7501 tcgactgata taactttttc aaaatcggaa tcattgcata aacataagcc atattcatct
     7561 gacgttcata attccaagaa aactccattt gaaaagaacg ccaaaatacg tgatttagct
     7621 cttttttcgt tattttgctg tttttgatct cagaattctt catcttcttc tacctcccct
     7681 gcagttgttt gaagtggtag ttgacggaca ttcataatat tgactaccac aactgctaaa
     7741 atcgcaccaa aaatagctac tccggtcaca ggaatctcta agtaagaagc tatagcaaaa
     7801 cctaagaaaa aatatggtgc aacttgtttg ttgatcagca aacgagcaag catcgcaaaa
     7861 ccaagagctg gaatcaaccc tgtagctact gacaacccct tttgaacgaa gtcaggaatc
     7921 atatttaata gatttccgat cgtattgctt cccaccatat aagaaaccat taccacaaca
     7981 ccaagcatca aggaaagacc gataccggca agcaagtgca tatgctcgac tcctttataa
     8041 tttccttctt ctgcataatt gtctgcttta tggttcataa ttggaatcaa aagccctaag
     8101 taaatatttt tcaatatcaa tgtcaacgta gcaatcggca aacctagtaa aagcgcggtt
     8161 tctgtaccgg ctccagctgt aatagcaaaa gccgtcccta aaatcccacc ggtcacaaca
     8221 tcaggtggaa tcgaagcacc tactgagaac gatccgataa atgctaactc taaagttgcg
     8281 cccataatga tcccagtttt tacatcacct aacacgattc ctgtaaacag ccccgtcaca
     8341 atcggacgag aaagcaacga tgtccctagt gcatattctg attgtgcgac aaatgcaaca
     8401 attcctaata agactgcttg tacaatcatt gaggttcttc ctttcttaca aaacttctct
     8461 tacatccact tttttatctg ctggtacttg acggatttct acttctattt gtttgtcaat
     8521 taatcgtttc aagcgttcct cctcttctgg taaaatattt accgcttttc cgatgctgcg
     8581 agtaccaggt tttgccttga tccctcctaa gttgatggtt ttgatttctg gatacgcttc
     8641 tgccagctta tatgcatcct cgatcgattc cactacgatg aataacttat atttatctgt
     8701 caccccgctt ttcaacgcgg caatcgagtc atcaattgat ttgatcacta gtttgactcc
     8761 ttgtggcttg gctaatttga tcgttgtctt tcgtatctca ttcgtaggta catcatcatt
     8821 agcaatcaaa atacagtctg cgcctattgt ttgtgtccat gaaaaagcta cttgtccgtg
     8881 taataaccga tggtcgactc ttgtcaatac aatcattttt cttcctccta aaattcgtct
     8941 tcttctattt tctgattgag ccgctcgtta caaaactgga tcattttttt tgaatcagtt
     9001 agcacctgct ggatcaaaac agttgtctct acttcagcgt ccaaccgaga tactagctca
     9061 atcaatagtg gcaaattcaa tccggcaacg agatagacat gatcccgatt gatatattgt
     9121 aagaattcgt tgttgacact tccgccaaaa agatccgtca gaacaatgat ttctttatct
     9181 ttgtacctca taaaaagctg ccttactgtt tccgctaaat caaaattctc atcagtatag
     9241 caatcgaata cagtaataga atgatttttc ccatagatca gctctaatga atctaatatc
     9301 cctgaagcaa attttccatg ggaagctaag ataatttctc tatccacttt ttcatctcct
     9361 cttttcactt acacatacta aaaaagcaag ttgcatgcca aaattagtgg caacaacttg
     9421 cttatgttca aaattcttct tctatcgaga gaaactctgt atcttcactc aaaatatcat
     9481 agatataggc aatctcagaa ttggggatca tgacactata atgctcttca atgacactaa
     9541 acgcagattt aatagcagat aacccttctt tttgacactg cttccatttc tctaatcctt
     9601 gataggtttc gattggcatt tgtcggatca atcgttcaat gagacagctc acatggacat
     9661 acaacgctaa ttttttggga ttgctgatgc ttcgattcaa ctgcacctct aattctctca
     9721 taaaatgatc gatttctccc atcaccttct ctgtatctag aatagtcacg gaatccagta
     9781 ctttttccaa tgaaaaatta cgaatgatgc gctgattgaa tatttccttc tctgaagcat
     9841 tcatagtagt gtccaaccat tcagctagta cagacatctt ttcccccgag atgaggtctt
     9901 ctaatgaaag atagtcgata ttggtgatat caggatctgt ggtgccaatc attcctacga
     9961 cttcgtatat ggaaaataat gggtcactgt ttttcttatc ttttaattgc tgatattcat
    10021 atgggatgat tttcaactga caagccgtag gtaagctttt ttctagcagt tcacttaagt
    10081 ggaccgccgt accgattcca gtgaagcaag tagtaatgag tgctttgggt ttgttttcct
    10141 ctggatagag cagtcgccat tctggctgga tctgtcgaac agtctcttct gctatctctt
    10201 ctaatgatac atttttttgc aggcattccc ctactgtgat tgctaaaggt gtcgtcacat
    10261 tattcaaaat caaaagcggt acggataact gttttttaaa aaactgatgg atttctttca
    10321 gagatcccat gtctactaat atgatcaatc catttgaaac atcgctgttt tcgcaataat
    10381 caaggatttc tgaagcaatc tgctgtggtg tgacatctag tggcatatca aatgattcaa
    10441 atagatcctc ttgtaataaa cgatttacta catttgccat actgctagca gttgcataac
    10501 cgtgagcagc aatcatcgct tttggcagtc gtttttcttt tgaccatttc gcttttttta
    10561 gatagatcgt cagtaaaatc cgatccatac ttgtgtcttc aatatctaat ttgatcttac
    10621 ttaattccag aattcgttgg acatagtgat aactagatgg ataagactga gaaacttgtt
    10681 gttctaattc tttgattagt tggataatat catgttcttc cggtaaccat ttgatagtgc
    10741 ttcgctgata gagatagtaa ctcaaggcat agacactgtt tccatcgaat ttgatttgat
    10801 aggcagtttc catttgtcga aaggtctctc ggatagattg tgtccaataa accagcagct
    10861 cgtgtttttc ttgtctactg gtctcaaaca gcaaaaaatc aaataattga atcacttctt
    10921 gctttaattt tccttcgcaa gaatatagca gacagttatt tttcttgaaa tcaattagga
    10981 tttgctcaaa acttttaacg attcgctgct gttcgggctg ttgcttctcc attaattgct
    11041 ctaaggtctg gtcttgcgta atatagattc cttgctgtgc tgtattttta gtaataggct
    11101 gcgcaagtag ttctttttgc aaatgatgga tcgtgacatg gatttcaggc tgttcacgct
    11161 gttcagcata tgcttttgct gctgaaactt tgacgatatt tttcagctct cctatgtttc
    11221 cggataattt ctgacttgtt aaaagtgaca aagctggtcc actgactgac aaagggctcc
    11281 ccatttttct ttgttcttcg agaaaaaaag agtaaatcag ttctagccgt tcattacgac
    11341 tacgctgatt aagtgcaggt agttcgattc ggataggtat tcttcgaatg aaagttgtta
    11401 agaaatgact agtaatctct tcagtcgtag caaaaaagag acgtgttttg atccggcgtg
    11461 acaaattagg ttctcccata cgataaatca caccttgatc aagataggtg aacagttttt
    11521 cctgaccttc tggtgttaag cgatgtacct cgtctaaaaa caagatccca ccatctgctg
    11581 cttcaaaagc ccctttttta ttttcttctg caccagtgta cgctccctta acatgtccaa
    11641 acaaattgct agtcaaaagt tcaggattat ctgcatattg tgcacaatta aaagtgatga
    11701 atggtgcaga atcttctagc agatcatgaa gcagacaata ctgatagacc aaattaacta
    11761 aataactttt acctgtgcca ctttcgcctg taatcaaaag tggtaatccg ccatctggat
    11821 agtaaagagc tgttttgatt tgttcgatac tttctttcaa actggcgtcg tatccgatca
    11881 taaaagagaa taagtctttt ggttgcgcga aaaaaggctg ttcctctttg agatcatcga
    11941 tagaaaaata aaaattcgtg cgtaacttaa aaaattgttt ttcaaaagct tctttgtgga
    12001 aataaatcac tggtcgagaa ttgattttga ctagtagttg ctcatcattt aactggttca
    12061 aataatgact gactgtgttt ctttttaccc caaaatgatc agctaacctt tgtgctgtaa
    12121 agacctcgtt catatgttct aaatcgaaaa aggctgtctg gttctctaag taacataaaa
    12181 tttccttctt caacaactta ttcacccctc ctccatcata gaaaacggat tcattttgtc
    12241 aataaaatct ttaataaaga attgaaaaat atatcttatt atagacagca gcctacttat
    12301 cccttataat ttttattcta aaaaacgtac agttccttcc agtttttata ctggaatttc
    12361 aacatatgaa agaataagga ggctatgact attcaatgaa atcggataaa tataaaaaca
    12421 ggaaacatac ttcctggaaa acttttcgag aaatcatcgg atcttatctc gctgtagttg
    12481 tatttttgcc tttgctttat atggctatga cagaacaaac tcgttttatt ggcgtagata
    12541 cgcaatatat agccgaaaca ggatttttat tcatctttat cttgattttt gcgttctata
    12601 aatccttcca tacaaaataa gattcacaga tttcaacttt cctgtctgtt tttagctatt
    12661 acaaatcctt atatgtggta taacaacaat ttattggcat aatgaggaaa aacgagttat
    12721 gatgctttca gagagtaaag aggtactgtt gtcaccatcc tttttattga ttacgtacac
    12781 cactcttagt tttttcccat tgattacgaa aacaaggtag aaacattgat gttcctacct
    12841 tgttttcttt ttttattttc agatcaaaat ctcaagtagc ttttgacttg atgtcgggag
    12901 gttgtcttcg ttccatcaaa cctgatagaa tctgcgccaa accatagttt tctccactat
    12961 tatttattcc tctaagatag tacccacaat aaacgaataa atatttttct cattttcata
    13021 ctccttcttt gaaaatcaat tagatttctc tcaacccact tttttacaaa aattacgtag
    13081 caaaaaagat
//
LOCUS       DACDCJ010000078        12510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDCJ010000078 DACDCJ010000000
VERSION     DACDCJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.078"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            53..526
                     /locus_tag="IV038_002269"
     CDS             53..526
                     /locus_tag="IV038_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP8342458.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            535..762
                     /locus_tag="IV038_002270"
     CDS             535..762
                     /locus_tag="IV038_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342459.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1398..1769
                     /locus_tag="IV038_002271"
     CDS             1398..1769
                     /locus_tag="IV038_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342460.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2025..2267)
                     /locus_tag="IV038_002272"
     CDS             complement(2025..2267)
                     /locus_tag="IV038_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8342461.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2299..3201)
                     /locus_tag="IV038_002273"
     CDS             complement(2299..3201)
                     /locus_tag="IV038_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8342462.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3214..3402)
                     /locus_tag="IV038_002274"
     CDS             complement(3214..3402)
                     /locus_tag="IV038_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8342463.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3416..3979)
                     /gene="amaP"
                     /locus_tag="IV038_002275"
     CDS             complement(3416..3979)
                     /gene="amaP"
                     /locus_tag="IV038_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8342464.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4017..4910)
                     /locus_tag="IV038_002276"
     CDS             complement(4017..4910)
                     /locus_tag="IV038_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8342465.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4988..5926)
                     /locus_tag="IV038_002277"
     CDS             complement(4988..5926)
                     /locus_tag="IV038_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP8342466.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5960..6310)
                     /locus_tag="IV038_002278"
     CDS             complement(5960..6310)
                     /locus_tag="IV038_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP8342467.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6343..7245)
                     /locus_tag="IV038_002279"
     CDS             complement(6343..7245)
                     /locus_tag="IV038_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8342468.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7238..8095)
                     /locus_tag="IV038_002280"
     CDS             complement(7238..8095)
                     /locus_tag="IV038_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP8342469.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8429..9238
                     /locus_tag="IV038_002281"
     CDS             8429..9238
                     /locus_tag="IV038_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8342470.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9278..9775
                     /locus_tag="IV038_002282"
     CDS             9278..9775
                     /locus_tag="IV038_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP8342471.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10160..10233)
                     /locus_tag="IV038_002283"
     tRNA            complement(10160..10233)
                     /locus_tag="IV038_002283"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10197..10199),aa:Ile,seq:cat)
     gene            10420..10743
                     /locus_tag="IV038_002284"
     CDS             10420..10743
                     /locus_tag="IV038_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342472.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10907..11161
                     /locus_tag="IV038_002285"
     CDS             10907..11161
                     /locus_tag="IV038_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342473.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11231..11476
                     /locus_tag="IV038_002286"
     CDS             11231..11476
                     /locus_tag="IV038_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342474.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11552..11917
                     /locus_tag="IV038_002287"
     CDS             11552..11917
                     /locus_tag="IV038_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342475.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3608 a         2332 c         2135 g         4435 t
ORIGIN      
        1 acaataatta ttctgctata ttttagctat caaagataaa agagggataa aaatgaaatt
       61 taaaacattg ctattaaaat ttgtcgtttt aggtataagt gccgtattta ttttgttcgg
      121 cgtacttttt tatactcaga tcgctacaag cgaaaaagga ttcaattttg attggcagac
      181 cacattattt gtttctgtca tttactttgc ccttctccta gggatcgtta ttgcttattt
      241 cttattgaaa ctattgaaaa aaatcgaaga taaccaagct ttttccactc aaagcttaaa
      301 catactaaaa aaaatcgaac gaaccatttt gtttattggt ttcgcctttt ttggcttact
      361 tcctaaattt atgaaagtag cggatttaga cgatgctcca ggtttgatgt tggtaggaat
      421 cgctgtcgta ttcattcctt ttgcagtata cacattaaca agcgtgctgg aaaaactgct
      481 tgaacatgcg attttactta agacagatca tgatttgaca gtgtgaggtg cgacatggca
      541 attatcgtta atttagatgt agtattagct caaaaaaaaa tgagtgtgac tgaattgagt
      601 gagaaagtgg gcattacgat ggcaaacatc tctattctaa aaaatggaaa agcaaaagcc
      661 atccgttttt ccactctcga aaaaatctgc caagttttgg agtgtcaacc aggtgatttg
      721 ttgacctata aaaatgactc gaatactata ccagaagaat aaaataaagt agaacaaaca
      781 gtaaataaaa aataatctga atgatagatg tgtgagactt ttagaagtta tttacgtcta
      841 ttattcagat tttttatttc gattgaatac cgttttatct ataaataaga tatgtactga
      901 ctcatacaaa gggggccttt tcttctccat gtactatcac ttttcctgat acaactggtt
      961 tcctgttacg ttgtagttgg atctgctttt agtataatac tttattcatt ccaccagact
     1021 gatagcagtt tgagcagtct aaataatcat gtagtattac aggccattct gtttcatacc
     1081 ttgaagtgct taaaacttca gctaatggcc cattgtgtta actcttactc cattcttttt
     1141 tagtaagctc ccatatatac tgtaaagcct tctatatatt atgtcatgtt gctttttaac
     1201 atttactatg tagttttgat aaaatgattt gaataacagt gatggacaat tcttttttat
     1261 ttttaacatt attttgataa taatgattat ttatttaaaa atacattaga taacatttgt
     1321 tacgacacga aaaagcttca caaacaaata tcgttatttt aaaatgaggg cgaactcaca
     1381 caattaggag gaatagtatg ccatttattg tttacttttt tctttcatta ctcgtgatct
     1441 atattccatt accaacattt ttcctgctcg tcaatcgtct agcagagcaa catgatacga
     1501 ttgctgctct tacgaaactt tgtttacttc tatttgtttg tctagattac tttacgagct
     1561 attattggct aaaacagaca agagtaaaaa agagatatta tttcttcctt gtctttttca
     1621 atctctttga gtttctgatc cattgctttt tacttcatca gtatcatacg atccatttgt
     1681 ggatcatctg ggttttcaaa gtggtcgtat tattttgtat gttcaccttt cctatctcaa
     1741 gaaaatttag ggaggcattg ttttattaaa gaagcttctc tgtatcaata aagacacatt
     1801 taaggaaaaa cttttaaaaa tctaattggg aatttgcccg cataaataag aggctgtgac
     1861 aaaagtcttg tcacagcctc ttattccgta taaacagtgt tcaaaagctt gcatcttcga
     1921 taatatgccc aaccaaatga aaaatatgag aagctatttt tgtttggctg ttcttattac
     1981 ttgatgaaga acagcttttt cacgacctct ttttaaaagt ctttttacag ttttcgagca
     2041 aagaatgata cgattagtac taaaatgatg gcaccaatca atgatggcac gattgccatt
     2101 cctgcaatac ttggtcccca agaacctaat aatgcttgac caagtgctga tccaactaaa
     2161 cctgctacga catttgcaat aatccccatc gaagaacctt tattcgtgat tgctcctgca
     2221 attgctccga taattcctcc gacgataagt gaccaaataa atcccatgtt tcgttcctcc
     2281 taatgtttta cttccttatt actgaacccg tggttctgta ttttctttta ctttttgtgc
     2341 gccgcttcca gctgctgatt ttactttatc tgtttgattc gaagcaaatt caccggtttt
     2401 tttcgctgcg tcagttactt tgtcttgcac cgtttcttga tctttttcaa attcttcttt
     2461 tgttttgatg tccactacgt tagcgttgac ttcgatcact tctaaatgtg tcatattttt
     2521 tacttctttg ccgattactt cttgcatttc ttcaaagatt ttttcgatat ctttgccata
     2581 ttccacaatg acatccatat caactgctac ttgtttttta ccgacttctg tttcgatccc
     2641 agccgttaca ttatctgtat tcacaagttt tcctgccaca ttagagaaga aaccaccgtt
     2701 tgcagataat aaaccgtcta cttgttcaat tgcgattccg atgatttttt gcacaacttt
     2761 atcttcataa ttcagctcac cgcgcattga atcttcctgt tcagaatcat gctttccttg
     2821 gttatttgta tgatcagaac caagttttcc ttgtccgttt gtgtggtcag aactaagttt
     2881 ttcttgtgct ttcgcatggt cagaaggatg tttttgctct tccacaggtt tagaaggatc
     2941 gattgcatca tgttgttctg gaactttaga ttctggagtt cctggatgat gaggtgcatg
     3001 cttttctgca tcatgtttct tctcttctac tccttgttga acgttaggga ctactggatc
     3061 tggatgtgca gatactggtg ctgttttgtt cttttcatcg ttcacaccat ttgattggtt
     3121 tggtgtcgtt gggtctggat gtgcagacac tggtttttgg ttgttcattt tattgttatt
     3181 atttttgtta ttgttattca tggatgtttc ctcctaagat ttaaaatagt gatctaagat
     3241 tttcgtttgt ttcagataaa aaccgatagc gacgccgatc gctgccatga tcaagatcaa
     3301 taaggtcttg aaaaagccca cggtgatcca taaaacggct aatatcaatc caagtacccc
     3361 aaagatgata gggagcttgt atgtcgaaaa tagttctttc atttttggtc cccccctatt
     3421 ctactcgttt gcggtcattg cttcgtttag ctggttcttc aaagcctgtg tatttcaccg
     3481 atactttcac tggctctttt gttcccaata tgtgttgtac ttcattttcg atttctttca
     3541 tcaatgttcc tgttttacca atgagtgctg aagttcgttt caactcccct tttacattga
     3601 cggcgatctt gtttttcgtt gctttgacat ttacttttgg tgaatggatg aattcatctt
     3661 catgcaagtg agaacgaacc attccttcaa ttgctttttt atccaacgtc aactccccat
     3721 caccatgttt cagtagaaag tttccgattg tttttggata aaacaggaaa acaagtaacg
     3781 tgataattgt caaaatagca aatgcaacgg caagccaaaa tacatattgt tgcacgtatg
     3841 aattagtgat ggaaaagaat cgaaacttct ctagctgaaa aggcaatgga taggttgcac
     3901 ttaatagtat cgcaaacaaa agcacagata gcaaaagtaa tgttccgatc actccgatcg
     3961 tttttacacc tttattcatt ttcgcacctc ctcatagggg tgaaacgatc aacttcttat
     4021 aagttgatcg cttctccacc agttactccg taaatttggg ctgtaacata acttgcttta
     4081 ttcgatgcta agaaaacata aactggtgct aattctcctg gctgacctgc acgttctagt
     4141 aaactttgct gaccaaattc agggatcgaa tcagaaggtt gtccttcgtc tagttgtaat
     4201 ggtgtccaga ttggaccagg tgctacacca tttacacgga ttcctttagg tgcaagctgt
     4261 tttgctaaac cgatcgtaaa gttagcgatt gaagcttttg ttgctgcgta atctaataaa
     4321 tgttcgcttg gattgaatgc ttgaacagaa gttgttgtta cgattgctcc tcctgctggg
     4381 atatgcggaa cagcttcttt cactaaagca aacatagcga tgatgtttat cataaatgtg
     4441 tctttgactt gctcaagtgg taactcagca atagactgac aagaaatttg ctgtcctgcg
     4501 tttagtacca tcgtatctag cccgccaagt tcactcacgg ctttatctat gatttcttta
     4561 ggtgcttgtt cgtcacgaag atcaaaaggt agtaagacaa tcttacgtcc tgcatctttc
     4621 acgtattttg ctacttcctc tgcatctttt tcttctcctg ggaagaattg aagagcgata
     4681 tctgcccctt ctctagcaaa tgcaatcgct gctgcacgac cgatccctga atctccacca
     4741 gtgattagta cacggcggtt ttctagttga ttattgcctt cataggattc ttctccacaa
     4801 tcaggaacag gaatcatatt ctgctgtaaa gctggtgtat cctgatcttg tttaggaaat
     4861 ttctccgtat ggtacaagtt tcgcgggtcg attaattttg gttctgtcat tgctgtattc
     4921 ctcctagttt ttaaattgta ttgccaaaac aaatttcttt gtttgatacc tgttttaaat
     4981 agattcttta atttgtataa tctttccggt ataaatggta acctaaaact actgctgcta
     5041 tcaaagagcc aaagactacg agtataaagc ctagagcaga acttttaccc ggtaatcctc
     5101 ctgtattcat tccccaaatc cctgcgaaca acgccggaac ggaaatgatt aatgcagctg
     5161 aatctagata tttcatgata tgatttagat tattgtccat catcccgttg aatagatcac
     5221 caatactctc taataaatct cgatagatct cgatcatttt ttctgtttgt cgctgtctca
     5281 aatggacatc atagagtagc tgttcatcgt ctaaacgttt gatgaaagtt tcagaattcc
     5341 ataattgatc cattgtttcc cgttgatctc ttaaggtatg atcgatatat acgatgtctc
     5401 tttcaagatc tgcctgttta tacagttctt cattttccgt tgttttacga gctgctttat
     5461 ccaaagcgtc aattcgcttt ttcatctctc ttagttcttt cacgtaatgg gtgtaaatat
     5521 ctaataatgt atacgtgatg atcttttcaa acgagtttaa atgatttcca tgctttgtca
     5581 gcagccgatc cacaaattcg gtttttctat cgatacaagt aatcaagaga ttatcggaaa
     5641 gtacaaaaga tattggcgtt aatttttttt cgatacgatt atctgactga tctagattaa
     5701 tcactaccaa agaaaatgga tgctctagtt tcgtctcata aaggcgctcc atccgagaaa
     5761 cttcttccgg atattcggtt cctacgaaaa tatcatcagg aagctcgaat tcttcgattg
     5821 cttgctcaat ctcttcgggc tttgcactat caagtacgag ccaattatat tttttttcat
     5881 ctgtttctat tacattcttt tcttcatcca caaagtagta tctcaatgta tacctccttt
     5941 ttggactttc tcgaaactat tagctgaaga agcagttctt taatttgata ttataatcga
     6001 gtactgcgat ttcattttct aatgaaaagc tgtcaagctg tttgttgaag tccatgttca
     6061 atcgtttacc gaaataattt tgctctaatt tagatgtata gatgtccaat aatggatgct
     6121 cgattttagt cgcatattct ccaaattccg aaacaaaagg aatgatttga aaaaagctgc
     6181 cttttgcaaa agcacctttg tcccttagaa atggatcgga atcattgata agcgcgagtg
     6241 cgatttttgt attttcccca accttttcaa ttaacgtctc tactgcattt ttagacattg
     6301 taatttccat tcctactccc cctcttcagt tgttgtcttc tattatttaa caactacatc
     6361 tgtttttggt tgaacttttg catattttgg atatttaggg aatttagcta cacaacggaa
     6421 agccgtcatc cctgcatatt tttcgtaata tttcttcgtt ggcccttcaa aatccttgct
     6481 ccacggtttc acatgtcctg aaaaatggat aatgtaagga ttgttgcgag tttcttcgta
     6541 ttcacgttca cctgctgctg ttggatgttt ttttgctttt gccatgatat agccttgtgc
     6601 attccaacgc ggatgaagtg gtaaccaacg gtcatgtaag atagcattta atgcatcttg
     6661 gtcatggaat cgtaattttt ctggattatg ttcgatgaaa tctaatactt tttgcgtaat
     6721 attttcatct aaccattttt tcacattgat cagcatcaaa cctgagttga aataacgcat
     6781 tgatttagct gaaattttca ttttttccag tcgttgatgg aacccagcgt cttcaacagc
     6841 tgctacgatt gaatcaccga agtcaagacg ccataattta gagatatctt gtaaagcgat
     6901 catgtcgcaa tccatatata agatacgttc aacttctgtg ccgcggaaca attccggaat
     6961 cgcaatacga taataagctg tttctggaat acgatcgctg attaccacat ttgtaaagaa
     7021 ttctttattg attttcaaga attccacatc tgagttcatt cgtgcgtttt tcactgagaa
     7081 gcgtaaaagc tgtttacttt caaaatcgat atcatcgtca atcacataga accgaacaaa
     7141 tttagacggg ttgcaattat ctaatactga gacaaataat gcagctaaat gtggtacgaa
     7201 ttttgtgtta caacttgcga ctactgctat ttctttttta ttcattgatc tcgcctactt
     7261 tcgttagagt actgtgtgct aattttttta agtataaatt ggtatacgga tgatctttca
     7321 acgtattcca aggtttatca tgtcctgtaa agtgaacgat cgaaggtttt tcatttcctt
     7381 ctttatactg acgttcatat ttttcatttg gtgctggatg acgttcaaaa atcagcgaag
     7441 tctgcatgtt ccattttgga tgaagttgtt cccaatcttc ataaagtaca gcattcaaag
     7501 catcttggtc gtgatagatg attcgatcgc cattttcact gaggtaatgg atagtttttt
     7561 ctgtaatttc ttttttattc cattgatcaa tgtcaatcac catcactcct gaattgaagt
     7621 acaagtcatc ggaatcgatt cctaatcttt ccaaagcttt cacttggcct ggatcgataa
     7681 tagcgccgat tgtttttcca tttaacggct catcatatag ttttacgata tcgtctagta
     7741 ctaacacatc tgaatctaag taaaggactt tcttataatc ctctttagct aataagtttg
     7801 gtaaagaaat tcttaagtaa gcggtagttg tgatatggtc actgactagg aagtcttcgt
     7861 agatatcttt ttccactgtc aggaattgga tacttgcatt acttgaatat ttatttaccg
     7921 tttcttccaa tccttcttta ctatattctg atagaccatc gtcaatcaca taaaatttta
     7981 tgcgtcttgt tttattgcaa ttttctaatg cagttgcgat catcacgctc aagtaaggag
     8041 cataattttc gtctgaagca gttaccacag gaactacgcc gtatctcttc tccatcaaaa
     8101 tcactccttt ttctaatctt tttctaacct gttctacatc tagaatattc ctcatttggt
     8161 tttaaagcaa aatatatgct ttgttttcat tgaatatttt aaaatttttt tatcacaaaa
     8221 cgcatcggta agcgctaact attcgttttt catggctttt tattcaaagg tattgaccaa
     8281 aaactactat atatagccac ctttttctta agaatgtttt ttgaaaaaat tcataatgac
     8341 aaagcaagtt agtttcattt ttttaaattc tcatttaatc ttaaggtaac aggaaaatag
     8401 atatgtaaaa agaaagtagg aagataaaat gaaactagca gcgattgatt tagacggtac
     8461 cttgcttgat cgaaatggaa agataccaga agaaaatatc caagcacttc ggaaatttga
     8521 tgaaaatggc ggaatcgtaa ccgtcgctac cggaaggaac agcatttctg ctaaagatat
     8581 tttttccgaa ttaggaatcg gtggctattt gatttcttcc aatggttcac tgattgctga
     8641 aatgaaggat ggaaaaatgg cgaaagttct cagacgttca aaaatagata gcgatacact
     8701 gagaaaagcc ttcttctatg ctcaagaagc gaaaatatcc atcattgcta gccgagaaac
     8761 ccaagatgat cagctgacat tcgatgaatc aaatctagtc aaagatgacc cttattatga
     8821 acatttcaat ttacagaacc attcttttga tcaaatttta aatatgttgg aagatccttc
     8881 tcttagctat cttaaattag cattaacaga taagagtgaa gaaaaactac agaagctaca
     8941 aagagatctg caagcagatg gtatccaatc agtattctct gatccgcatt tcttagaaat
     9001 cacgcctaca ggtatcacta aagctcattc acttgttttt ctggcggatt accttggaat
     9061 cgaatcgagt gacatcatgg cttttggcga tcaagaaaac gacattgcta tgttgaatta
     9121 cagcgggttg agtattgcga tggggaatgc acagcgacaa gtaaaagaaa ttgcaaacca
     9181 agtaacactg acgaatgaag aagctggtgt tgcttatatt ttaaaaaact tctattaaaa
     9241 agaataaaat gacgatatga atgtaggagg aaagaaaatg ttagttatta ttgatatgca
     9301 aaatcatatt cttgatccaa acagcgaatt ttatattcca gagtcagatc aattggcaga
     9361 acgtattgct caaagattag ctaaagcacg ccaaaataat gaatatattc tcttcacacg
     9421 ggatatacca gttgaactga aggacaaaga agaagaacga gaagatttgc aattgatccc
     9481 tcttctttct ccacaagaaa atgaacgagt gattaaaaag tattacttta cgattccacc
     9541 tgaaacttta gctgaaatcc aggaaacttt atttgaaagc aaagaagaac aaaaagagat
     9601 cgaagtagtc gggattgaaa caaacttgtg tgtactctct aatctgatag gcctgcagag
     9661 tgcttttcct gaagcagact tttttgttga tccaatgtta gtctccagta gaaaacacgg
     9721 agaatccgct ttagagctgt tgaaagattt caacgtctct atcgtcgaat cataaaaata
     9781 tctatcttca tttacgatat ttaacacaca ataaaaaaag cacgcagaac tgttttagtc
     9841 ctacgtgctt tttctatcaa agtagattgg cttttttatg acacagagtg aaacggcagg
     9901 ctcacgacct ctttcttaaa tggtgttcaa tcagctgacc ctcggtatta gcttaacaaa
     9961 cggcaaaata tgaggaacta tttttccgtt tgttaagcta atactcaggt caaaacgctg
    10021 atttcacaac ctcttcctta aacggtattc aaaagcttgc atctgcagta ataagaacag
    10081 ccaagtaaaa acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
    10141 gctttttcac aacctcttat gtaccttgta ggactcgaac ctacgaccgg acggttatga
    10201 gccgtctgct ctaaccaact gagctaaagg tacatcataa ataaaattta tttttgttac
    10261 ttttcaacta tagcacgaat gacaaactct cacaagtttc aaaatataag taaaataata
    10321 ttgttattct tatttctttt acttgtgtca taactatttt gttactttaa tttttatttg
    10381 cttattttag aaaaaatgtt attcttttat ttattctcaa tgttaggagt gattattttg
    10441 ccatttattg cgattttatt cgatttagtt gcggcagcag cttattttct ccaattaaat
    10501 tatcaaacct caggaattct gtttattgga atgatttttc aaggtgtgat tacccttttg
    10561 cttcttgtca tgatgctcag ctataaagga aaaagatatg cccgtattca aacaaaagta
    10621 tttgttaaat atgtttccat tcgctatggg atcatcattc tttccttttt agtgaatgcg
    10681 atcgtcctct ttttatatgt attgaattac ttagatatca atccattgat tttttctcga
    10741 tagataagac acattcagcg gaacagttcc tgcttatcag cagaaaactg ttctgctttt
    10801 tcatttttca ttgatctatt cttcttttat tttcctagca tttcatttgt gatgtatgca
    10861 ataacatgtt atcttttatt atatgaatcg ttgaggagtc cgttacatga ttacgttggt
    10921 gagagttttg ttttgggtgc ctgcggtcgc actggtcgca agtattgttt atcttatgaa
    10981 ttggaacaaa gaacggtttt atttagctat tttgactctt ccggctatct actttatgtg
    11041 gaaagttttt aattacaact actttgaacc cgatagtgtt tttatagaag aattatcagg
    11101 actggttttg tctttattga tcgtcattct ttatttgata cgtttaaata aaaaacattg
    11161 agtactattg attgcatgtg tagctttcta tagtcattat aactattcta ctataatgaa
    11221 aggtgattaa atgagtatcg aagaagctat tttactggat actttacgtt gtcgtctgaa
    11281 tttcttaaga actggccgat ctttatctca agtagctgtt tctagcgcgt taagtattcc
    11341 tttattcaaa tatcgtaagt acgaagaagg agaagaaatt ccaagcatca ttgaattaat
    11401 ctatatcgct gacttttacg atatttccgt tgattaccta attggtcgct cagaaacagc
    11461 aagcaaactg aagtgatttt ttccagcctt ggtgctggtt ttttcattta acgtctaagg
    11521 ttaacaatct atatataaag gagacatttc tatgcgatta tggcatgaaa aattgatttc
    11581 aaagcttcct cgtcaacagc ttttaggaca acatcgggaa tgctgtgcat taagaggaaa
    11641 tggctgggga aagaaacatg ctactgtgga ttatgttttt cactattctc cttatagatt
    11701 attccaatat catcagcttg tcatggatga aatggaaaaa cgtggttaca gcccagcacc
    11761 agagtggaaa aatcctatgt atcgtggcaa atcatgcgaa gcattttttg atttacctac
    11821 tgtagagcgc aacgtgccga tttatccgga acacgatgaa gtttatctag cagaatgttt
    11881 agcaaatttg gagcaaaaag gaatttatct tacctaatat aactagaaaa gccttctatc
    11941 tcctcttact tcattaaaaa gctttaacca tcttagatca agctaaaatg tcgtaagttt
    12001 tagggttatc ttcatcctca cgtgcaaaac taaaagcgta ctctagtttt ggttcatcgc
    12061 caacttccct agacataaat gaacatctcc tgacgtggtt taaaagtacc gcatcttcga
    12121 cgtctgtata cgatgcacag cacagattgc ttcttttgat ctttcttttg atttttctgt
    12181 tgcttcttct acaccttctt cgattactac atcgatgctt acaattgctt ctgcctaata
    12241 ttcccatgca tcgtcgattt aagcctctgt agtcactgtg atcgtttcca cattttaact
    12301 cctctgtcgt ggcttcagtt gattttgtga tcagttcagt tgctaagcca gagtcagaac
    12361 aacctgccat tgctagcgtt aatattgata gtaaaaatag tttttttatc ataaggttcc
    12421 ccccctgaaa atagactaaa ataataacgc tttctttttt tgttttcaga cgaaaattaa
    12481 taatttctaa atattcttta aaaaagctta
//
LOCUS       DACDCJ010000079        12100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDCJ010000079 DACDCJ010000000
VERSION     DACDCJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.079"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            713..2629
                     /gene="ltrA"
                     /locus_tag="IV038_002288"
     CDS             713..2629
                     /gene="ltrA"
                     /locus_tag="IV038_002288"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8342476.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            2746..3546
                     /locus_tag="IV038_002289"
                     /pseudo
     CDS             2746..3546
                     /locus_tag="IV038_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            3543..5939
                     /locus_tag="IV038_002290"
     CDS             3543..5939
                     /locus_tag="IV038_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342477.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            5991..6323
                     /locus_tag="IV038_002291"
     CDS             5991..6323
                     /locus_tag="IV038_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342478.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            6324..6917
                     /locus_tag="IV038_002292"
     CDS             6324..6917
                     /locus_tag="IV038_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342479.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            7080..9104
                     /locus_tag="IV038_002293"
     CDS             7080..9104
                     /locus_tag="IV038_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP8342480.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            9104..10309
                     /locus_tag="IV038_002294"
     CDS             9104..10309
                     /locus_tag="IV038_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP8342481.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            10325..10969
                     /locus_tag="IV038_002295"
     CDS             10325..10969
                     /locus_tag="IV038_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342482.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            10980..11786
                     /locus_tag="IV038_002296"
     CDS             10980..11786
                     /locus_tag="IV038_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342483.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            11809..12039
                     /locus_tag="IV038_002297"
     CDS             11809..12039
                     /locus_tag="IV038_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342484.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKIRSFEALYLAAKKYFTAERK"
BASE COUNT         4425 a         1922 c         2545 g         3208 t
ORIGIN      
        1 gtgacctgtt tagcctaagg ataaaagagg ctagttccaa cgaacattgg aataacttta
       61 ctatcgagaa atcaaatgac ggggtaacgc cctgaagggt ttccactgat actgcgacat
      121 gcgttagtag gatgtgcatc caaacgcgta tgaagctcgc tgaagtcggc agaagggtac
      181 cgtaaggaaa gacacgatac gtatctacca cgcaagtgct ctagaggtag ggtatgtacc
      241 tgatatgtcg ggtgtcctaa aaagttcaat aaggtgagaa tgatttgatt atctgacaaa
      301 cttgcgaatg gaatgtctga aacgaataac tcatagaaat gtgaggggga agggggatac
      361 gtcctctttc cattgactcg aggttgagta aaaatgtgtt tctatgaaaa accttgcatg
      421 gttaaaggct atggagtagt caacaggcaa aagcaagcac ctaagttgaa atctatggat
      481 agatagtaag gaacagggga agcatctagc acggagggag cataccgaag atgtgtttct
      541 aaggaaaaac cgaaatcggt tataacttag gtgaatagat gtgaaagtag agccacgacc
      601 agtgaaaatt tcgtaatgaa attggaggaa caggctcaag tcgttattta taatataaga
      661 aacctattta aaatcatgga ttctcgtaag actaaaaagg tcacgaacca gaatggagcg
      721 tgataaaacc atgactaaca caaaaggaat tagcttaaag aacaaacaac tcagacacgc
      781 cgaatactac gacatgactg agactttcga tgaattgcac gcaatgagtg ttgcaaaatc
      841 aaattttaaa aatctcatga aattcattat tgatgacaat aatatctttc tagcgtatag
      901 aaatatcaaa aagaataaag gaagtgcaac ggccggatac gatggtgtca ctataaaaga
      961 cattgaaaaa ttggcatgtg gtgacttttt aactattatt aaaaggcgat ttaaggatta
     1021 cagaccaaga aaagttaaaa gagtagagat cccgaaacca aacggaaaac ttcgaccttt
     1081 aggaattcct tcaatatggg acaggatagt ccagcaatgt attctacaaa ttttagaacc
     1141 aatatgcgaa gcaaaattta ataagcatag ctatggattc agaccaaatt gttctacaga
     1201 aaacgccata gcagattgtc ttgctagaat caatcaaagt aagatgaatt atgttgtaga
     1261 tattgatata aaaggattct ttgacgaagt aaaccatacc aaacttatgc gtcaaatatg
     1321 gacaatgggg attcgagata aacaactcct tgtaattatt cgtaagatgt taaaggctcc
     1381 gattgttatg cgaaatggca atgttgtttt tccaaccaag ggtactccac aaggagggat
     1441 tttaagtcca ctgcttgcga atatatgcct gaatgaattt gattggtgga tagctaatca
     1501 atgggaggaa cgaactgtca aagagctgaa acaccactat cgaaaaaacg gagcatggga
     1561 taaaggaaat atctattcga aattaagaag ccacacaagt ttaaaagaga tgtatctggt
     1621 acgctatgca gatgatttta aaattttctg ccgaacagga gaagaagccc aaagggcttt
     1681 ttatgccaca caaaaatggc tcagagaaag attaaagctc ccaatttcaa tggagaaatc
     1741 taaagttacg aacctaaata aagctgaaag tgaattcttg ggtttttcaa taagactcca
     1801 caaaaaggga aaaaagaaag tggctttcac gtacgtttgt cgcaaagcag aagaaaacat
     1861 taaaaataaa ctaaaacaac agattaaaaa aattcaatac gctggagcaa aaaaaaatag
     1921 gcaccgagca ataggtgact acaacatgat ggtcatcggt atccacaatt actatcggat
     1981 agcttcacat tgtagtgtca atttcactga gatttataga agtgttagga cagtaataga
     2041 agcaagactg aaagctagag gaatgacgaa aaatggaatc tatggacaag atcatgaagg
     2101 ataccgtccg tatctaaagt ctaagcaaat gagatatatt gaaggttttc cgatacttcc
     2161 aattggctat attaaaaaaa gaactccact caacaagcgg agaaatctaa ataaatatac
     2221 caaggaaggg agaaagctca ttcatcaaga gcaacaagct atagaaagct ggaaaatcaa
     2281 gtatcttgta aaaggtttta gtaacaataa gaaatacacg gtggaatttt ctgataacaa
     2341 aatatctaag tttatttccc aaaaagggaa atgcgctatc acaggaaacg aacttttgat
     2401 ggaagaaatt gaatgccatc acattaaccc atatcatttg tccaaagacg atagttacaa
     2461 gaaccttgtc attatctcta gagaaatcca tagattaatc catgcaacta gtgaagaaac
     2521 tattctgaaa ctgcttcggt tactgaaact aaatcactat caaattagaa aactaaacac
     2581 gtatcgaaaa aaagcagcta attttccaat atcaatcaaa atgaattaga taggaaacaa
     2641 attatattgt aattaacgat ggaacgccgt gtgcggtgaa agtcgcacgc acggtgtgga
     2701 ccgggggaaa agacggagat aatatcaaac tcttacctat cggaattgcc accaatggat
     2761 aatatggaag gaatcatgac agtcacagct ggtagaaaca tgttgtggga tctgtttatt
     2821 caatcctatc agcaacttta tgcgaagtat ggggaacaaa acgcagcgat tatcaaagaa
     2881 aattgtcaga cacatattta tattatgtcg acagacgata ataccatcga agaattttcc
     2941 aaaaaagtgg ggaataagac ggttgaacaa gagaacagta ccaccaattc attaggtatg
     3001 aatactcata tcaatcgcaa tgttgattca gatcgtatct taactcctga aagaatttct
     3061 acttttttag aaggtgaaac gattgtactc gctcctttaa aacgacaaga tttgaaaggt
     3121 cataaaatac ggccgtatcc tatttttaat catggggaaa cgaatcttcc ttatgcttat
     3181 caatatattg aagatttcaa tccatcgtgg gatatcaatg atatagacat acattgtaca
     3241 catgcggact tggatcttaa ggctttacaa attgattacg attattttct acctgatatc
     3301 tatgcacgtc gactttatca caatatgcac gatcaaatag aagaggaagc taaagaaaac
     3361 atttttatta aaatcgttca atctttagac aactcaacgg cacaaaagtt acttattcaa
     3421 ttttatgaag agaagcaaat agatcgtttg caagaatacc ttgaaaaatt gcatcaaaat
     3481 gaagaaatta cagaggttat aaggaaacga cttttgctag aagctgaaaa ggcggtgaaa
     3541 gaatgatcat tggaggaata ttttccggca ttgccaactg gtggtcaggc ttatttgggg
     3601 atggggatgc agatcccaaa accgtaacga aagtcttaca agattatggt aattatctgc
     3661 agtatcaaga tgtattcagt tggatttgta atactttttt atggggtttg attaaagggt
     3721 tatataatct caatacttta ctagaaaaaa caatttatca aagctttgat ttaaaagact
     3781 tattgaacgc tgctggtgtt aatgaactgt ttcaaagttt gattagtaag gtattggctc
     3841 ttttttgtgt cgtcacatta atttatttag gcttaaaatt ttttatgagt aaacatccac
     3901 caaaaatcaa aaatatcttg gttaatctat ttatggccat gattttgatt tttggtggta
     3961 gttctttaat cgatgaaggg ctaaatatta gtaaaaactt ttacggagac gtgactgcag
     4021 caaataaagc agataaaact ggatcacctg cttttcaaat tattcaaaat aatgtgatcg
     4081 atgtgagtac gctactagaa actgatccga ataaagtaga atccctacca acagataaaa
     4141 gaaatgctct cacggcgaaa aactttaaga cagcaaatat taattcagtg attattccta
     4201 aggaagccgc taagatggcg gatgaagata agagcggtct atcagatata caaaaggatc
     4261 ggctaagagc gttacaatac cgactggaac tggatgataa tggaaaagaa atccctgtag
     4321 agattagtga tgaggggtta gcaaaatatg tgtatacttc aggctatcgc cgatacatta
     4381 gtagtccagg gattatttta gctggtgaat cttctttgtc ggttgcctat ttatttatct
     4441 tgtttacgat tgtaacttgc attattgagc tggtatttaa aaaattctat ttagtaattg
     4501 ccgcgtcaac ggactttgag acaggacaac ggatgaaaag tgctatagaa gatatttctc
     4561 aatccttctt gttattggcg tttacgattt tagaattacg aatctataca ttaatgctgt
     4621 caggtatcgg tgatttgcac gcagcaggca agatcaacgg atttttgtat gttgtcgctt
     4681 taattgtact aaccattgcg ctattcaaag gctctcaatc tgttacgaag attttcggtg
     4741 ttgatacaag tctgaaaaac ggcagcaatt ctcttcttag catgtttgcc ttaggaaata
     4801 ttgcgaaaaa tgtgggtagt ggggctaaaa accttgcaag tgctggtaag agtggtttaa
     4861 gcaatttaaa tgatattcgt aaaaatggat ttggcaatcg aaaagatgtt attcctgata
     4921 acggggaaga acaaccagac gataaagcta caattgtacc taatagtaag aaaacagttt
     4981 ccgaaatgtt cgggaaagcg aaaaatggtg tttctaaagc cgcagaaggt cttggttacg
     5041 tcaatgaacg tgggttaaaa ggaacagctg aagatcttac agaaaaagcc aaagacacct
     5101 tagaagagaa attagacggc acgagagcta aagaattggc tgaagctgtg aagaatccat
     5161 ccgaagcact agaaaaacaa aaagaaaaag cttcagaagt caaagaagga ctaaaagatg
     5221 cgtatggtga aggacaagtc aatgcagcag tgaaggcaaa tacaacaagt gaaaaagaaa
     5281 atcaagaagg tgcagcgatt ccttcagcca aagatcacca agatgagtta cattctaacg
     5341 aaaattcgat cggagaaaaa gattcagaag aaggacaaca tggacagtta ccaaaaaccg
     5401 atccagaaca atatcgttat aaatctaaca ttaattctga aaatgaaaag cgcatttcag
     5461 atttaccaaa acgaaatcaa gaagtacaaa cggatgagga ttccatcgat ttgccaaatg
     5521 ataatggaat cgaagttgga actgaaaaat caatgaaaat tggtttgcct aagacacaaa
     5581 atttaccaat gaaagatagt cgtttgaatc aattcaagga gaatgatcaa agctttgttc
     5641 accaatcaac tgcaggtact gttcaaatga aaccaagtcg tttgcctgaa aatggaaatc
     5701 tgacaactgt ttcttacaat ttatctgaaa gtggtgagac agggaatgtg tccattcaaa
     5761 atgttgtgac aagagaagct gcaccagaac ctattcaaaa agttcacaca ggttacaaac
     5821 cagcaaatgt ggcatttact gcaccaacaa aaataaatgt aacaagagaa actaaaaaca
     5881 ggaacaatga attgaaagaa caactattga gggatatcaa acaggatatc aaaccataaa
     5941 aggcctacga gttaggcctt ttttctatgt atgaaaagga gaaatcattt atggataaat
     6001 atattaacat gaaaaatcct ccaagattta gtggggaatg gaagtttttc gcctttttct
     6061 ttttagatct attaacgctt ggtggttgct ttttttgggc gaaaatagtc aataatcttg
     6121 tccacttacc actctttttt gcactcttga attatggctt atggattgcg atagctctag
     6181 tgattgtttg gcgaccttat aataacccag gtcaacgaca aatttttgcg attatcgaat
     6241 ctattttctt tgcggataag aaccactatc atgcgttaga tcctaaccga aattatcatg
     6301 gtacgtataa ggaggatgaa taaatgcgag tcaaaggaca aaattcagtt gataagaaat
     6361 ccattttgca gtttggaaaa ccagaagaac gatctatctt ggatttattt ccttataaat
     6421 cctgtacaga agagggagtc ttagttacta aagaagatca ttttcaacgt tattatcgtg
     6481 tagtttctac agacgtggaa ggattaaacg aacaagagaa actagaacga atgaatcagt
     6541 tgaccaccgt catgcgtacg tatgtgcaaa caattaagtt agttagtttg accacagaga
     6601 cggatctaac ggaacaaatc gtacaaaagc ggcaattatt agataagaat cgtatggaac
     6661 aatccttagg aagaaatcta cagcgattaa gaaaatatga aaagaaatta gtagaggaaa
     6721 tccaggaatt gaaacgagca gaacaagaac gtcctgactt aagcttcttt ttcgtcattg
     6781 aaggaaagac aatcaaggat ttacaaacca gaaatcgtca gttaatgcga tctagtggca
     6841 ttttagggtt aaaaccactc tacaaaaaag aattgatcaa gattctttat cgaatgaaca
     6901 atatgaatga tgagtaggaa tggagaagag agaatgaaat atccgttgat atggttagaa
     6961 aatgatgttc cgaaggaaga gatcactaga aaattttctc acactttaag cctgggtaag
     7021 ttgggaaaag ggcaatcttt taaccaagta aagaatcaaa caagaagagg tgactgtaca
     7081 tgttagaagc gattaaaaca gctttttcac cacaaaatga tcaaaaagta cttgatccat
     7141 taaagcaact aggaaatcgg gaaatagaaa ttttaaaatc caaagggtat gatttagatt
     7201 ttttaaaaca agtgatgcca caaggtggat taaatttcga tgaagagtat gtgacctatg
     7261 gaaatggtta ttcacgatgt gtgacagtct atcactatac aaaaaatcct actctttttt
     7321 ggttggccaa tctcatgaac aatcctcata cagtggccac actagatgtt agcacagatg
     7381 aaaagcaaaa agtgattgat caaatcaacc gcgcattaga tgaattaaat gatcgatcag
     7441 ataatgaacg aaaagcgact gaccgaaata aaagtgcgga agaatacctg gaactagcgg
     7501 aatatgcccg aaaattgact tctaatgggg aaatttctaa acaagtcaaa gtgagagtac
     7561 atgtttacgg aaatacgata caagaagtgg atgaacgggc gcaggaactg atcgaaaatc
     7621 taaaagggat ggattatcgt gcggtgactt atctgcataa aaccaaacga gattggcaat
     7681 cgatgtttac catgttcgat gaacaagctg aatggttcgg aaatatcaaa gggcaatcac
     7741 taccatcgtt aaacattggc ggaggtttcc cgttccacca ccaaacgcta aaagatccag
     7801 gaggtacaca atatggggaa accatgacag gcggcccatt tgtctggaat gcgacgtata
     7861 aaaatagtgt acggttgtct actaatacct tgcttctagg aatgatggga gctggtaaat
     7921 cgaccgttct taaaatgatc gcggaagccc atttagctgc aggagacttt atttggggct
     7981 ttgaaaaggg aaaagacttt attcctttct taaaggaata taacggtatt atggtgcgtt
     8041 tagacggatc tgacgggatg atcaatcctt tagagatatt tgccacacgg acgtatgatg
     8101 aagcatccag gttatatgac gatggatcag tcaaagattt gaagatcaat gaagcagcgt
     8161 cctatcaaac gcacttagac aaagtagtct atcaggtaca gttagtgagt cctcagctaa
     8221 aaggaacgat gaaagcagaa tttaagacct acttaaatcg tttttatgaa gagtatggaa
     8281 cagtaccacg tggctttact tcctcaaatt cgcgttcaaa tactgatacg caagtcacag
     8341 gtaaagatcc agaagcttat ccgacgttca aagaattttt agacttttta ggacaactgg
     8401 aattaccagg tgcttcccaa gagaaaaaga atcgaaaaga agaaatggaa tccatcgtag
     8461 aatccttatg tgagacgtat ggcatgattt ttgatggtca cagtacgatt cgacaccttg
     8521 accaacaaca gttggtcttt tttgacattg actctattac aggtttagat cgaggggtgc
     8581 aacaatgtca aatgtacatg gctatgacgt tgatttggaa tcaggcattg atccagggaa
     8641 gaagacaacg acgtttaatt aaagaaaaac aaatggaaga acaagacttc aaacgcttct
     8701 tggcgatgat tgatgagtgc cataatgcga ttaatccaga ttttattgaa acggtaaatt
     8761 atgtcacaaa ctttcaacga gaaatgcgta aagtagaagc aatgactgtt ttagctacac
     8821 aatccccaca agaaatggtg ccagaaaata tgtcttctga taacttctcg aaactgaaga
     8881 aagtctttga attttcttct acgaagatct ttatgcgtat ggatgaatct attttacgtc
     8941 atatagaagg actagtgggt aaatcaatga cgaactcaga attagaatcc attcctcaac
     9001 aaaatcaagg agacgctgtg atcaactttg gatcaaaaga aaccattcgc gttcatttta
     9061 gtccgaacaa acgtcaatta aaattgtttg atggaggaaa ataatggaaa atcaagatga
     9121 aagtttaatc aagcagtatg ttaagcgtcg cgcaaagcgg cgcttatttt tgtggttgtt
     9181 tggtacttct gcaggtttga ttactatttt gatcactgtg tttgttaccc tatttctaat
     9241 acttgcggcg ggttcgattg ataattcgga ttcagattca agcagtggtg gcgaagcttt
     9301 tacaggagaa tattcggaag gcttaccgat ttataaagaa atcaaaggaa gaggcccttt
     9361 ttctgatgag attgcgcaat atgccgtagg tgcagcagtg aaatataaat tattaccaag
     9421 tgtgattctt tcgcaatacg gttatgaatc agcgtttggt acgtcggcgt ccgcaagaaa
     9481 tgatctcaac tattttggga ttacttggtt tgatggttgt ctgtttccta aaggaactgc
     9541 acgaggaatt ggtggcattg aaggtggctg gtacatgaag tttcccaact caaaagctgc
     9601 attctcctat tacggcttta tggtagccac acaatcaaat tttaatgcat gtgtaggaaa
     9661 taaaagccct ggcgcaagct tattgatctt aggacgaggt ggttatgccg ctgcaggaat
     9721 tactgaagat agtccgtatt atacaggttg tatgtcgatt attacctcaa ataaattaac
     9781 agaatatgat gaatttgcga tcaaacattg gggagaaggc ggaaatgaca acggtacgat
     9841 tactggtgaa tggacgaatc ctttccctgg cagcagttta gataaaagtt ccttcagcgg
     9901 tggtcaatta tttggaacga atccaggcgg tgaattccgt ccgaatggtt tccatgatgg
     9961 attagacttt ggttcagttg atcatccagg aagtgagatt catgcggttc atggaggaaa
    10021 agtcgtgtac gttggaaatc ctggtatttc aggtttgggc gcgtgtgtga tcgtgattaa
    10081 ttacgacggc ttgaatatgg tatatcaaga atttgcaaat agtacaggaa attcccgtgt
    10141 gaaagtggga gatcaagtga aagttggtca agtaatcggg atacgagata cagcacactt
    10201 gcatttaggc ttcactcgaa tggattggcg tcaagcacaa ggacacgcat tcacagatga
    10261 tggtacatgg atcgatccat taccattttt gaatagcagc aaaaaataga tcaggagaaa
    10321 aaatatggaa aataaaacgt taaaaatcgt cttaacagct gcactcagca ttagcttaat
    10381 tggcaatatc gcgctctttt ggcagctgaa agaggaacaa gcgacaaata cctataaaat
    10441 tgaagaagtg gctaaaaacg cgtctgagaa gctcaaaaaa aatttctctg aggcacaggc
    10501 gaagatcgac aagttaaatc aaaccatcaa tgagaaaaac caagaaatcg atcagctgaa
    10561 gcaacaagac ggtataaaag caaaagaaga agtcacagat gcccaaagaa agacggcaga
    10621 agagttcggt caattggcca ttgataaatc actaagcaga aatgaattaa cggaaaaact
    10681 aaaagatgtc gcaacccaag aagtgatcga caaattaagt cctgcagatg atgatcacca
    10741 acatgatgat tatggaagtt actcctttac attaggtgac gtgcaaactt acgctgaaac
    10801 aagcagtgta aaagaggaac aaaattttgt cgtatttgtt gattatacaa tcggcaatcc
    10861 tcaattcaaa gatgtcaaac cgcaaaaaat aaaaggtgga ttaactgtca ctgaaaaaca
    10921 agtagatggt caatggaaag tcacagactt tagttatttc actcgttagg aggaagaaaa
    10981 tgagtaagaa aaaaggagca agacaacgtt ttaaaaaacg gaaaagttat cttagatggc
    11041 tgggaatctt tacgattctc ggctttttga ttcttgctag tccgatttat acgcataaga
    11101 aggtaaacat accagatgag gtaagcacga acgctttttt aaacacgaaa gaactgacaa
    11161 tggtatcaaa agaaaaagga gcgaatcagc aattagtttt tgaatttgcg attgacgatg
    11221 aggatcaagg cttattaaaa gaactggcta atcttgatta tcgcgtagaa gtaaagacag
    11281 ctaaaggtga ttatcaagca ataaaagcca aagtgatcaa ggtgtctgat gattattttg
    11341 tagtgaaatt agcacatgta ccagaagaat atatcgccat gcgtttaacc attgttcctg
    11401 aaaaaattga tccaaaagtg gatatgcaag aaccacaaga tctgatttat tacgttcacg
    11461 aagataaggt taaggatcaa gtgaaagatg ataattatga acaacatgcg atcaattaca
    11521 aagtgaaagg atacaagaaa gaacaaaaag cagcacaaaa acagattgaa tccttgcaag
    11581 caaccattga tttgaatgaa gaattagtga ataaactaaa agaccaattg ccttatcaag
    11641 tcgcagatga tcaggaaaat actgaaaata agattaatga ttaccagcaa gaaattgaaa
    11701 catcgaaaac gcaaatgaaa gatcaagaag agatcattca aaaaattcaa gaaaaaattg
    11761 atcgtatgac aaaagaaaat atttagtaga acaggtggag tgaaagaaat gaagggagcg
    11821 aacttggtcg gcaaagatat tttcgctgag cagaaagtat tgattaaagc ctatctcaaa
    11881 gttgtaaaag ctaaagaaat atgtcaagaa gtgaatttat ctaacacaac aatatctgag
    11941 ttgaagaatg gtagaaagag tttagatacc ttaaaaatca gatcatttga agctttgtat
    12001 ttagctgcta aaaaatattt tacggctgaa agaaaataaa aacaggagga ttttatgtga
    12061 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt
//
LOCUS       DACDCJ010000080        11745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDCJ010000080 DACDCJ010000000
VERSION     DACDCJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.080"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(179..586)
                     /locus_tag="IV038_002298"
     CDS             complement(179..586)
                     /locus_tag="IV038_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP8342485.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(775..1602)
                     /locus_tag="IV038_002299"
     CDS             complement(775..1602)
                     /locus_tag="IV038_002299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP8342486.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1911..2129
                     /locus_tag="IV038_002300"
     CDS             1911..2129
                     /locus_tag="IV038_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8342487.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2150..2785
                     /locus_tag="IV038_002301"
     CDS             2150..2785
                     /locus_tag="IV038_002301"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8342488.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2978..3217)
                     /locus_tag="IV038_002302"
     CDS             complement(2978..3217)
                     /locus_tag="IV038_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342489.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3241..4764)
                     /locus_tag="IV038_002303"
     CDS             complement(3241..4764)
                     /locus_tag="IV038_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP8342490.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4782..4904)
                     /locus_tag="IV038_002304"
     CDS             complement(4782..4904)
                     /locus_tag="IV038_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP8342491.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4934..5917)
                     /locus_tag="IV038_002305"
     CDS             complement(4934..5917)
                     /locus_tag="IV038_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP8342492.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IV038_002306"
     CDS             complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IV038_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP8342493.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6111..6488)
                     /locus_tag="IV038_002307"
     CDS             complement(6111..6488)
                     /locus_tag="IV038_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342494.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IV038_002308"
     CDS             complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IV038_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8342495.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IV038_002309"
     CDS             complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IV038_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP8342496.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7506..9278)
                     /locus_tag="IV038_002310"
     CDS             complement(7506..9278)
                     /locus_tag="IV038_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8342497.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSRTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9232..10986)
                     /locus_tag="IV038_002311"
     CDS             complement(9232..10986)
                     /locus_tag="IV038_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8342498.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11096..11665)
                     /locus_tag="IV038_002312"
     CDS             complement(11096..11665)
                     /locus_tag="IV038_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP8342499.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         3704 a         2265 c         1695 g         4081 t
ORIGIN      
        1 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
       61 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
      121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
      181 agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac ttatggggta
      241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
      301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
      361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
      421 tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt gtttcattct
      481 ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa atgccaataa
      541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
      601 taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
      661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
      721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
      781 cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
      841 taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa aatcactatg
      901 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
      961 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     1021 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     1081 gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     1141 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     1201 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     1261 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     1321 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     1381 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     1441 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     1501 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     1561 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     1621 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     1681 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     1741 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt catagggaac
     1801 ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga
     1861 tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt
     1921 attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa gaaggatttt
     1981 ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta catgaactga
     2041 gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg cttatcgaag
     2101 aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca
     2161 tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga agcatgaaca
     2221 tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg atcttttcga
     2281 tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa
     2341 aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta ctcattcgat
     2401 ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat
     2461 tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa aatcatgctt
     2521 ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat
     2581 tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa
     2641 agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct tttattttcc
     2701 agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa
     2761 tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt
     2821 gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc
     2881 tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc agatcaaatc
     2941 caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc
     3001 gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc
     3061 gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta
     3121 atgtactccc atttaatctc atttgctttg caaattgata acgccgataa gcatcaatat
     3181 tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa agattggatt
     3241 ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg gatagaaagt
     3301 aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa agaaaatatg
     3361 gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat atttaaaacg
     3421 accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata
     3481 attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac
     3541 aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt
     3601 ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt tgtaatcgaa
     3661 aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata
     3721 atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt
     3781 ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttctc
     3841 taacgctttt agattgtctt ccattactga aacataatct tcaccattat ccatttgttc
     3901 ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt cattggccaa
     3961 agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt
     4021 tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag
     4081 tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa aggctgcatg
     4141 ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa
     4201 ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg
     4261 atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga
     4321 aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc
     4381 ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc
     4441 tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac
     4501 aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg
     4561 ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt
     4621 gaaatcatac attggataga atgttgttac aatttctaat tttccatctt tatcattcga
     4681 tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat
     4741 aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt
     4801 ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt gatattcggg
     4861 cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa ttacccctaa
     4921 tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt
     4981 cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt cgagtggtgt
     5041 attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct tgatcttgca
     5101 aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccggtcaa
     5161 ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa
     5221 actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt tctgatgaat
     5281 cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc tttttttgaa
     5341 tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc
     5401 tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat
     5461 ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac
     5521 tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta
     5581 aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg
     5641 aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat
     5701 cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat
     5761 gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt
     5821 cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc
     5881 ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag
     5941 ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc
     6001 taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc
     6061 gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct
     6121 tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt
     6181 gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat acattgaaat
     6241 aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag
     6301 ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa
     6361 cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc
     6421 gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata
     6481 atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat
     6541 tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc
     6601 attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg
     6661 tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa
     6721 cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg
     6781 gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc
     6841 agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct
     6901 tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt ctttgcttta
     6961 gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat ttagacatct
     7021 caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa
     7081 caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc
     7141 ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct gaaaaataag
     7201 aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact
     7261 taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt
     7321 tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga
     7381 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
     7441 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
     7501 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
     7561 tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt accttgatca
     7621 actatttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
     7681 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
     7741 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
     7801 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
     7861 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
     7921 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
     7981 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
     8041 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
     8101 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
     8161 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
     8221 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
     8281 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
     8341 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     8401 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     8461 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     8521 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     8581 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     8641 tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg
     8701 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     8761 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     8821 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     8881 acgtcagaca tgatagttcg agataaatct gataaattat gttttgaaaa ataggataat
     8941 ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc tttataagta
     9001 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaacg
     9061 aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa ttcgtctatc
     9121 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa gctagaaata
     9181 gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt tttataaaaa
     9241 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     9301 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     9361 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     9421 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     9481 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     9541 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     9601 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     9661 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     9721 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     9781 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     9841 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     9901 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     9961 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
    10021 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
    10081 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
    10141 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
    10201 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
    10261 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
    10321 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
    10381 tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
    10441 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
    10501 tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata aaacaggtgt
    10561 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
    10621 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
    10681 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
    10741 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
    10801 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagacttaag
    10861 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
    10921 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
    10981 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
    11041 tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact acgttctatt
    11101 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
    11161 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
    11221 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
    11281 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
    11341 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
    11401 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
    11461 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
    11521 tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac gccccaccaa
    11581 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
    11641 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt
    11701 aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttat
//
LOCUS       DACDCJ010000081        11644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDCJ010000081 DACDCJ010000000
VERSION     DACDCJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.081"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(274..759)
                     /locus_tag="IV038_002313"
     CDS             complement(274..759)
                     /locus_tag="IV038_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP8342500.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1239..1880
                     /locus_tag="IV038_002314"
     CDS             1239..1880
                     /locus_tag="IV038_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP8342501.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1899..2534
                     /locus_tag="IV038_002315"
     CDS             1899..2534
                     /locus_tag="IV038_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP8342502.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2552..3367
                     /locus_tag="IV038_002316"
     CDS             2552..3367
                     /locus_tag="IV038_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8342503.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3686..5122
                     /locus_tag="IV038_002317"
     CDS             3686..5122
                     /locus_tag="IV038_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8342504.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5311..7758
                     /locus_tag="IV038_002318"
     CDS             5311..7758
                     /locus_tag="IV038_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP8342505.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7988..9055
                     /gene="rlmN"
                     /locus_tag="IV038_002319"
     CDS             7988..9055
                     /gene="rlmN"
                     /locus_tag="IV038_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8342506.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9248..10351)
                     /locus_tag="IV038_002320"
     CDS             complement(9248..10351)
                     /locus_tag="IV038_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8342507.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10904..11452
                     /gene="aac(6')"
                     /locus_tag="IV038_002321"
     CDS             10904..11452
                     /gene="aac(6')"
                     /locus_tag="IV038_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8342508.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3873 a         1957 c         2507 g         3307 t
ORIGIN      
        1 agttagaata taatattttt tgttaaatat gactgaaatc aaaagaataa aattccgatt
       61 tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat caataaatat
      121 ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata ctagttgtta
      181 cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg
      241 cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg
      301 ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt
      361 gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg
      421 gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc
      481 taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc
      541 agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt
      601 tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac
      661 atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt
      721 tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt
      781 tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa
      841 agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg
      901 gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc
      961 gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata
     1021 aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt
     1081 tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt
     1141 tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt
     1201 gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta
     1261 tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt accttgattg
     1321 gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac
     1381 gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga
     1441 tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac
     1501 tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac
     1561 aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa
     1621 cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg
     1681 aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa
     1741 tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg
     1801 caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa gttgaaggaa
     1861 aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga
     1921 acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga
     1981 aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg
     2041 ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat
     2101 tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc
     2161 cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa
     2221 aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat
     2281 tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg attgccagag
     2341 cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg
     2401 aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa
     2461 ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt
     2521 ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt
     2581 gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg
     2641 ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat
     2701 gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt
     2761 ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt
     2821 tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag
     2881 agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc
     2941 taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa
     3001 tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa
     3061 aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag
     3121 acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa
     3181 cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg
     3241 tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga
     3301 gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag
     3361 aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac
     3421 agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac aacctcttgg
     3481 taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa aaatgtagaa
     3541 agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc acaacctcct
     3601 ttacataatt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg
     3661 aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga
     3721 ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg
     3781 gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac
     3841 gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa
     3901 agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg
     3961 acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat
     4021 tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca
     4081 agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga
     4141 aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa
     4201 tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa
     4261 acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt
     4321 tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc
     4381 aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa
     4441 ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga
     4501 agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa
     4561 accagatttt cttgggatca attactatca aacggcgacg aatgcattca atccattaaa
     4621 cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg
     4681 agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg
     4741 ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat
     4801 gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca
     4861 aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc
     4921 aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag
     4981 ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca
     5041 agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga
     5101 agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg
     5161 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
     5221 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
     5281 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
     5341 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
     5401 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
     5461 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
     5521 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
     5581 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
     5641 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
     5701 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
     5761 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
     5821 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
     5881 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
     5941 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
     6001 gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag
     6061 aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat
     6121 acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct
     6181 gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt
     6241 tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag
     6301 gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat
     6361 caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa
     6421 agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc
     6481 gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc
     6541 gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg
     6601 attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa
     6661 gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga
     6721 cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt
     6781 agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc
     6841 catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc
     6901 atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa
     6961 aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat
     7021 atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca
     7081 accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc
     7141 aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat
     7201 atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat
     7261 gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat
     7321 tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag
     7381 aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt
     7441 caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa
     7501 gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca
     7561 cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt
     7621 tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca
     7681 gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg
     7741 gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa
     7801 agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt
     7861 atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag
     7921 tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga
     7981 aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt
     8041 tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa
     8101 acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc
     8161 ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac
     8221 agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca
     8281 agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt
     8341 ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca
     8401 gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt
     8461 cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca
     8521 tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag
     8581 tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat
     8641 ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt
     8701 cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat
     8761 cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt
     8821 agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc
     8881 agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga
     8941 tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga
     9001 tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta
     9061 tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca
     9121 acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa
     9181 ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag
     9241 caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt
     9301 ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa
     9361 tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat
     9421 tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa
     9481 acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc
     9541 cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca
     9601 tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat
     9661 ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt
     9721 gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat
     9781 ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag
     9841 catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa
     9901 tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt
     9961 tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga
    10021 agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac
    10081 cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat
    10141 ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca
    10201 aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta
    10261 cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg
    10321 tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg
    10381 ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc
    10441 taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc
    10501 ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta
    10561 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
    10621 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
    10681 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
    10741 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
    10801 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
    10861 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
    10921 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
    10981 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
    11041 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
    11101 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
    11161 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
    11221 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
    11281 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
    11341 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
    11401 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
    11461 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt
    11521 gaatttttgt gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca
    11581 gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt
    11641 ctac
//
LOCUS       DACDCJ010000082        11616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDCJ010000082 DACDCJ010000000
VERSION     DACDCJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.082"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            109..480
                     /locus_tag="IV038_002322"
     CDS             109..480
                     /locus_tag="IV038_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342509.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            565..765
                     /locus_tag="IV038_002323"
     CDS             565..765
                     /locus_tag="IV038_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342510.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            899..2125
                     /locus_tag="IV038_002324"
     CDS             899..2125
                     /locus_tag="IV038_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342511.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2975..4299
                     /locus_tag="IV038_002325"
                     /pseudo
     CDS             2975..4299
                     /locus_tag="IV038_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            4292..4639
                     /locus_tag="IV038_002326"
     CDS             4292..4639
                     /locus_tag="IV038_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342512.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            4990..5811
                     /locus_tag="IV038_002327"
     CDS             4990..5811
                     /locus_tag="IV038_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP8342513.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            5831..6391
                     /locus_tag="IV038_002328"
     CDS             5831..6391
                     /locus_tag="IV038_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP8342514.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            7364..8131
                     /locus_tag="IV038_002329"
     CDS             7364..8131
                     /locus_tag="IV038_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP8342515.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            8157..8702
                     /locus_tag="IV038_002330"
     CDS             8157..8702
                     /locus_tag="IV038_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342516.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            8703..9269
                     /locus_tag="IV038_002331"
     CDS             8703..9269
                     /locus_tag="IV038_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342517.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            9284..9532
                     /locus_tag="IV038_002332"
     CDS             9284..9532
                     /locus_tag="IV038_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342518.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            9551..9790
                     /locus_tag="IV038_002333"
     CDS             9551..9790
                     /locus_tag="IV038_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342519.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            9807..10391
                     /locus_tag="IV038_002334"
     CDS             9807..10391
                     /locus_tag="IV038_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342520.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            10498..10779
                     /locus_tag="IV038_002335"
     CDS             10498..10779
                     /locus_tag="IV038_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP8342521.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            10769..11110
                     /locus_tag="IV038_002336"
     CDS             10769..11110
                     /locus_tag="IV038_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP8342522.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         4424 a         1484 c         2299 g         3409 t
ORIGIN      
        1 tcatgtgaat atgaacttaa caattaaacg tatttacgat cttttatcac ttatataaag
       61 agagaagtga taaataggac acaaaatttg gaaagtaggt aagtcactat gagagaacta
      121 actttatctt ctaaggaatt agaaaagaaa actgctttat taaacgaaat atttagtgct
      181 gattgtataa tccaaagttt aaaagaaagt tcattttcaa aactaacaga tcaggaaaaa
      241 atttcttata tgctaagttt agataatcta ggaactaaat atgatgaatc agcaagtagc
      301 gataagcttt tgattgaatt agtatcaagt cttagtagtt ttggaaaaat gaaaagattt
      361 aatgtgatat tagaaatttt tgcttggtta atcccttcat ttatttggtt tttttatatt
      421 ttcatagttg aatctgagtt aatagccaag gtctctcttg gactgttaat tccgatataa
      481 ataggttcag caatttactt taataacttg tatacacgta ttcaaataaa aaagcagtta
      541 ttacatgaag gattaagtgg taatatgtta aaaagaattg catgggataa agataataaa
      601 acagatattg agcttaaaga atataaatac aattatttct cgagatgtat agatagaaag
      661 ttactgttta aaaaagaagg attagaattt agttttgatg aagatgaatt tgaaaaatta
      721 gtgatggact ggcgtaagaa tttactggat acggtacaat tctaaaagca attgaggtcg
      781 aaattccgat acttgaatca tgatctatat tatcgtgtta tgagtgaaat ttctaataaa
      841 gataagatat ttcatttgga cagtcatgtg aatatgagct taacaattaa acgtatttat
      901 gatcttttat atgtttataa acattaatga ggtgctaaaa ttgacaacaa taactatgac
      961 aaaaaaaata gaagtatatg ttgatggatc acaagaagaa agggattcct tttttgattt
     1021 tttttggaaa aatagtagag aatatgtaaa agcgttgaat ctggtatata aacagctaat
     1081 gtttgaagag ttgataaaag atgcaattaa agaaatggat caaggttatg ctgagagaga
     1141 agaaagtatt caaaatcgga ttgaaaagtt agcatatact gatccaatag atcaaaaaaa
     1201 acttgataaa gcaaaagctg aattatctaa attgagaaat gaaaaaaata aagaagcgag
     1261 agaaatatta acgcaagcga tagggttgaa gagtcaaacg agaacacgag atgtagcaaa
     1321 aaaattagac tttgactttg gagattttgt tgattccgca aatatgaaag ctagtaaaga
     1381 ttttggcaat gattttatcg gaattgtgac cggacaaaga acgcctagaa tatataaaaa
     1441 tagaaacaaa tatggattac taccttttag aggtcgagat attaaaatcc ataaagatga
     1501 taagttttat atttatttac ctaaaggctt taggtttaaa gtgaatttag gtagcaagcc
     1561 aagaaaagca agagatgttg cttcagtttt acaacacatt atggatggcg attacaaatt
     1621 aaatcaaagt aatcttattt ttgatggaag aaaattattt tttaatctaa caatttcttt
     1681 tgaaaaaagc tataatgaaa tggaattcaa gaaaggtaat gaattgatcg taaactttgc
     1741 agatttgtgt agcggtgaat gtttaattaa tgataaatct gtaatgtctt ttggtgatag
     1801 ccaatatata agaaacttca aaagtaaaat tttagcaatg aaatcaaaag aacaaagccg
     1861 aggagtcttt tctaagggcg gtcatggtcg aaaaagaaaa ttagatcaag atcgatggga
     1921 agcaataaag aaaagagaaa gtaattttgt taaaacttat aataatcaat gttctgcaaa
     1981 aatcgttaaa actgctattc gcaataaagc tcaaattatt cgtatcattg aaccagataa
     2041 ggcttatggc gaatgggctt actatcaatt gaaagaacaa ctagtaaaca aaggaaaaag
     2101 atatgggata gaagtcagaa gttagtaaat taaacaatcg ttgatttggc aacgaaatag
     2161 tgagggaaaa tccactcact gaagggtaaa ctccgtcaag ttgtgtgacc agacggaagg
     2221 acgaatgttg ttgaggtttt agaaccatat aaaacagcat gtttctcaaa tttttaggat
     2281 aagggaaatt gctagcagtt tgttttagaa ccatataaaa cagcatgttt ctcaaatgag
     2341 tactttaaat taattgatgg cgttcctgct ttagaaccat ataaaacaac atggatttaa
     2401 aacaagaagt aagtcagttc tccttttact ttagtaagga aaaataaagt attctcaata
     2461 aaacttaaaa taagcaaacc tttgttttaa tagctaatcg aagtaaaacc caaagccata
     2521 tgaacccctg aatcagcgaa agtttatgat gatttggatt actaatagtt taggaattag
     2581 atggtaaatc taaattaaac aatcgttgat ttggcaacga aatagtgagg gaaaatccac
     2641 tcactgaagg gtaaactccg tcaagttatg tgaccagacg gaaggacgaa tgttgttgag
     2701 gttttagaac catataaaac agcatagttc tcaaattgtt taaaatgatt gtcaagttaa
     2761 aaaaatctgg gatcatataa aatagtatag ttctcaaata tgattagaaa ccctattaaa
     2821 gagcaatatt aagccaactt acttaaatcg tttaattaag aacaataatc tcttttagtc
     2881 agacttttag tctgactttt ttgtatttcc aaaatttatt ctttacaaaa acgaacatac
     2941 gtttgtataa ttgcttgtga atgggggaat aaaaatgttt gattacagta gagaaccggt
     3001 gaatgactat tttttaatcg acatgaagtc tttctatgct agcgtggaat gtattgaacg
     3061 aaatttagac ccaatgacta caccgttaat tgtcatgagt agatcggaca acactggttc
     3121 aggtctgact ctagcttctt cacctactgc aaagaaaaaa tatcatatta ctaatgttag
     3181 tagagctact gatttacctt atccattacc aaaagaactt catattgttc caccacgaat
     3241 gaacttatat ataaaaaaaa tatggaagtt aatcagattt ttagaaagta tgtcgcagat
     3301 gaagatttat tgatttattc gattgatgaa tcaattttaa aagtgacaaa atccttaaat
     3361 ttatttacta gttcaggaac aagaagcgag agaagaagag aattagctaa aataatcaaa
     3421 gatgagattc agaaagaaac aggtctaata gcaacagtcg gaataggaga taacccatta
     3481 ttagctaaac ttgcactgga caatgaagcg aagagtagta aagaatttat agctgagtgg
     3541 acttatgaga ctgtacctga aaaaatatgg aaaataaacg agatgactga cttttggggc
     3601 attggatcac gcatgaaaaa aagattgaat catatgggaa ttatgagtat agaagattta
     3661 gcaaattgga atccctacac aattaaaagt cagttaggtg taatcggact ccagttatat
     3721 ttccatacca atggaattga tcgtacggat attgctattc caccagaacc aaccaaagaa
     3781 aagtcttatg ggaattcaca agttctacca aaagattata cgaggagaga agaagtagaa
     3841 ctagtagtca aggagatggc agagcaagta gccattcgaa ttagacaaca caattgcaaa
     3901 actggttgtg tacgcttagc aattggtacg tcaattttgg aagaaaaatc aggtttctcg
     3961 caccagatga aaataccaat tacagataat acgaaagaat tacaaaatta ttgtcttttt
     4021 ttattcaata agttttatga ggggcaggaa gtacgacata taggagtaac ttatagcaga
     4081 ttagtataca cagattccct acaattagat ttatttagtg atccaagaaa aaagattgct
     4141 gaagaaaatt tggataggat tattgataag attagacaaa aatatggatt tacagctatt
     4201 atacatgctt catctatgtt agaaggtgca agaagtatta cgagaagcac tttaatagga
     4261 ggacacgcag gaggattagg aggtttgcat aatgattgaa tataacgata gaggacgttt
     4321 aaaatggtcg ggattttatt tgtctgatca tactgaaaga atcaatgcag acaatgtaca
     4381 acgtgctaat cataatttgg caaaagaaca aatgacgact gaagagattg cgaaaatact
     4441 gagaactgct gtcataaaaa atagacccgt ctgcattcaa aaagaggaac gaaacgctga
     4501 aggttactat ccagatgatg tagtaggaaa aattattgga tatgacgagc ttggtgtatt
     4561 tgtggaagaa gaaaaagttc actatgatga aattaggaat gtttctttct cggaattatt
     4621 aaaatggagt cagatatgaa cattattaaa aaatacgaat tcggttatat tacgtatgaa
     4681 cagctaatag aggaattatg gggatatggt caacaattaa ttaatgaagt ggggattgat
     4741 tgcttttgtt tctatattga atccggagca ggataccata agaacagata ctatatattt
     4801 ccatatccac caaaatagtc tcttagatat ttttaaattt tctccaaata ctttcaatta
     4861 ttgtgaaata tttcccttta attaatcaaa ttttttataa aagtaatata taaaataaat
     4921 tgcttgaagt attatattta gatatcataa tttatggaag aaaaattaat taaaaaggat
     4981 gagattataa tggggttact ggttccgaga gctaatactt atactatacc tttaacgaaa
     5041 cgccaattaa ttgctaaaag atatcaaagg attactagag ccatcaatag agagttttgg
     5101 aattctgaaa gtgatactgc tcacagttta tacgtaggtt cttatgggag aggaacggca
     5161 attagtacta gtgatataga tataatagtt gaattaccga tggcagaatt tgacagattc
     5221 aaaaattatt tatcaaatgg cccgtctaag ttattacaag ttataaaaaa cgcatttcag
     5281 gaaatactac caaatagtga tattagagct gatggacaag ttgttaaaat aaatttccat
     5341 gatggaatca aatttgaaat agttccagct tttaatgaaa aagattattg gggtgagtca
     5401 aaagggttta tttatccaga ttcaaacatg ggagggaact ggaaagctac aaatcccaaa
     5461 aaagagcaag aagccatgaa attaaagaat acaaaaagta ataatttatt atatgctacg
     5521 tgtaaacatt ttcgacatgt acgagataca gaatttacga gttatcatct ttcaggaata
     5581 gtaattgaca gtttcgtata tgaggctatg ggaaattgga aattcgttga aaataacagt
     5641 gggggtcaaa acattagttc tgtatcttat gagacagctt tattagaata ctataactca
     5701 cataaagtta tgggcggttt aaatttatac tctcctggaa gcaatcaatt tgttaattct
     5761 gatagtagta tcatatgttt agaaaaagta ttaaaaaaaa tagctttata aagttttatg
     5821 gaggaacaac atgggagacg aaaaaaagcg cttggaagtt caattacgtg aagcatatgg
     5881 acgagtaacg tatacttaca ccactcattt aaagcaagcc gataatatta tttctgacaa
     5941 taaaatttta aggtgtcttc aaattggttt gtcagccctt actactggag gggtacttag
     6001 taccattatt actgataata gtgagttaac ctttgttgct ggattatgtt caacaatttc
     6061 tcttggatta aacttatact ttaagaattt tgatcttgct gaagaagcta aaaatcatca
     6121 aattgttgcc gataaattat ggttgatcag agaagaatac gtaagcttac taacagattt
     6181 aaatcgttta agtgttgaag atattgtgaa aaaaagagat caattaatga acgaaactca
     6241 tgaagcatat aaaaatgccc ctaaaacaaa tagtaaaagc tatgaatcag ctcagaaagc
     6301 attaaaatct gaagaagagc aattttttac agaagaggaa ttggataaga tgttaccaga
     6361 gcatcttaga aaaaagagtt ctaaatattg aactcttttt ttatgcttaa aattctcttg
     6421 taatttttct attcatctat atttaagaag aaatatgatg atagaaacat ctcctaaagc
     6481 caccgcaagc cgttctaagc atagcttagc ccgacattgc tagatatgct catctcacca
     6541 tgaacgtttc ctaaagccac cgcaagccgt cctagccatt gcctagtccg acatcgctag
     6601 attagtgcat ctcgccataa aagttttcta aagccaccgc aagccgtcct agccattgcc
     6661 tagtccgaca tcgctagatt agtgcatctc gccataaaag ttttctaaag ccaccgcaag
     6721 ccgttctaag catagcttag cccgacattg ctagatatgc tcatctcacc atgaacgttt
     6781 cctaaaacca ccgcaaaccg tcctagccat cgcctagtgc ctagtccgat tttgcttgat
     6841 cggttccatt tctgtttatt tagtgggtca acaccgtaat cttaaatagc catcgcctat
     6901 ttaagattac taggtctgct ttgaaggtaa aaagtctttc attcttcaag acttttaggc
     6961 tttcaagcta cgcctgaaag cattgaaatt catcgctctt tatttggttt tgccatttat
     7021 aattccttac gcccattatc tttttcgcct gtcttgcctt gtcagaagtg tttagagctt
     7081 atctcatctt tggattaata caagtggcgt aaattttttt ctaaacgcaa gcgttacgaa
     7141 aataatttac ttaaacactt gtattaatcc ggcagataag aacgcattct aatcactccc
     7201 gacaaggcaa gacaggcgaa aaagataaat aaatgaacga aaggaagaaa cataaaatgc
     7261 aaaaccaaat agcgatgaag aaaaaaccac ctccctctaa aacaatccaa acaaaaaaga
     7321 attttgaaaa accaaaaatc aaaaacagaa agaagcgttc aacatgagag atagaatttt
     7381 agaagttaat caagaaatgg taaacaagtt aacaaatatc agtgggaact atgaaaagta
     7441 cgatggtact ttacttgata attatatttt tcacgataca caaaatatca gaattggcag
     7501 aagtaaaccg agagcgttca ttctcatcaa agagaaatat ttgaatgaat ggaatagcac
     7561 ctatgaaatg gttatgactg ataactttga aaaggtattg gatttcagaa atgctttgtt
     7621 gggttctgat atgggaaata gcagtcttga atctattgaa gttgaaaaca agaccgagaa
     7681 tgaaggtgaa ggattgatta agaatgtcct gaaaatcgaa caaaaatata tcagtgaaga
     7741 tagaagtttt tatagagtca caagctcggt tggtctagca aactttgcta ctaacttaat
     7801 tggaaacaat gctagtgaat ctttgctagt gatgtgtctg aatacaaaga atgaggtcat
     7861 tgcttatagc gaagttttca aaggtggaat taatcaatca gtagcgatac caagagagat
     7921 ttttcaacta gctttgctaa acaattcagc aaaaatcgca gtatcacata atcatccgag
     7981 cggatatgaa tttccaagtg aaaatgacaa aagttttact acgaatttga aaaacgcagg
     8041 aaaaacaatt ggtattgaat tgttagacca tatcatcgta acaagtaaaa acacaagcta
     8101 ttactcattt caagaaaatg gttctttata aaaattagag aaaagaggaa aacaagatgg
     8161 gcgaaagaac acaagcgtta attacagtca aatatggtaa cagcaaaacg aaagtaagcg
     8221 tgtcaattca ttatcagtgg ggatatggtc gattcatgtt gatggacgtt ttgaatcaag
     8281 gaatatattt aatcaataaa gcatatgact tacgagatga atcaagagaa gaattagcaa
     8341 aaggaatctt tgatagaagt acaggagtta tcaattttgt tacctatgaa tctggagtga
     8401 gtgcttatga tgaaacaggg tttggtgggc gctatgaacc ttatcaagaa acgattgaag
     8461 atgtgatgaa gttttcagat aacaatgatg gctatgctca tttagagatg gtgttcgatg
     8521 aaaatggatt tagtctagat gataaaacca gattgcgatt gtttgatttt gagggtcaac
     8581 agataggact aaacaagttt tgtgaaagcg gaaaagagtt tgctacgaaa gattttatta
     8641 agggatacag attattgcta gaaaattatg gaataaacct agaagaagag agcgtgaaat
     8701 aagtgtttga aaaaagaaaa gaccagttcc ctaatttaga agcgaaaagt caaaaagtga
     8761 ttgatggtga aatgattgaa ttattccaaa aaagttatgg aaccatagaa actaccgcaa
     8821 gttgcaaata ctactttcaa ggaaagagaa acacgtttaa aggctttgcc gattctgatg
     8881 aagaattggc aaagaaaatc attgattggc ttgtcagcag tgaacacgta gcaagagaag
     8941 ataccgttag aacaacgatt gagggtgaag tcctaacagg tactagatgt gtttgttgta
     9001 atcatgaatt tgtagaagat gaaggttgct accgaatcaa tgaatatggt gattttattt
     9061 gccagagttg ccacgagata gaatttccaa ccgaaagaag ttggtttgaa tacatgctac
     9121 aagattatga gatggaaaca gaaaatggtg aagtagtttt ttgtaccaaa gaggaattga
     9181 gtaagagaag cgatgatgaa attgaagatt ggtgtgtgtg gttagtaggg acagatgatt
     9241 gcgttgcata ttacacaaca gcagaatagg agtgtgaatc agcatggaag gaatggattt
     9301 tatagatcat gaagatttga ttgattttgg ttatacatgg aaaggaatgg taggtattag
     9361 cagaagtctt gctaatgcct tttacgaaag aaattatgca gtctatgtcc tttatgatga
     9421 caatacagaa agccttgttg atgaagagta taaattggat ttagagaatg ttttatatgg
     9481 gatagaaaaa gaagatttag caaaatatat tttttcatgg ttgggtcaat aaacgaaaga
     9541 gggtagcaac atgacaaaat tcaaaagtaa aggtatgcaa tggtgttctt attattatgt
     9601 tgggaatgat gaagagcttt tgagaaaagc caaacaaaaa ggttatccag ataagatgtt
     9661 aaaacagatt ggctattaca tgccagaaga gagaatagaa aaagaatctt gtaaggttct
     9721 ttttgatagt gaacagctat tagttttgtt aactcaaaat aaattgattt gtcaatttga
     9781 aaataattaa tatggggtga agaaccatga atgagaataa gatggtatta agtgaatacg
     9841 caagattgag aaccattaat tggttgaatg gtatgcaaag tagtggcgct tttgatggat
     9901 ttgatgagga gaatatggac tttgaaagtt tatcgaatga ggatattaga tattatttta
     9961 tcgaatggta ttttacagaa ccagatgaag aggtggaaga tgaaccagaa ttaattttag
    10021 aacgtcaaag aattttattc agtgaacaag atagtaagag gttgtatggt ttatatgact
    10081 tacaactgaa aggattaaaa gcgcagtatg ttgctgatag ttacgatgat atggtagaac
    10141 agggggcaga tagggttgtc acttattcag aagatgatga agaggaatgg gaaaactatt
    10201 cagatgatga gatatgcttt aatgatgggt atatgcctat tgaaatcacc gaagaagaat
    10261 atgataaagt gtcggatagc gatgagattg gtatgtgtac ggttgtaagt ggattggata
    10321 tagatgtcca aagagtagag gttgcatacc agaagatgaa agaacgtata aaaagtaaag
    10381 tagaatcttg atttgattgg attgtttgca aagtagctaa ttaagagtat aatataatca
    10441 taaatgtagt taatggatat aataatgagt atattatact caaaagaggt gaacgaaatg
    10501 aaggtaaaaa atccaaaaga aaaaagcaga attcaagtcg gaatagacaa ggatttaaaa
    10561 gaaaacgcag aaatgatatt ggaagagcta gggttaaatc caacaacagc aatcacaatc
    10621 ctttataagc aagtggtcgc tagaggtgag tttccagtag agataaagct aagcgaagag
    10681 gaaaagcaag gaatcagatt gcaacagcta actatggata tgccagttga tgttctagat
    10741 acagatgaaa agttagaaga gtggttcaat gaagcctaat gagatagtaa cggtctatgt
    10801 tgcatttaca gataaaaatg gtggtaaaag aagacctatt ttggtggtta gtgataaaga
    10861 agatagagta gaattttttg gaatcacaag tcaatatgag aataaatcag accaaataaa
    10921 gagagtgtat tttcctattg cagaatggga aaaagtaggt ctaaagaagc aatcatggat
    10981 tgacgtaggg tctttaaagg ctattcccaa aagccgagag aacatatcat ttaaaaaggt
    11041 tggtatgcta tcagctattg atacgaaaaa gctaaatagg tttatcgaga gattacaaag
    11101 aaaagtctaa caaggctttt ctttttttgt ttatatgtat attaagatat acgtttatat
    11161 aaacgtattt atgtatatta aaatacacgt aaatatatac gtgattataa aaattaagaa
    11221 aatattaagt attgttaaat agtgataatt agtaatatac gaattataag atacatttta
    11281 tatttaatgt atgtaaataa ataaaatgac tgacggatat tgaatagtaa taaataacaa
    11341 taattcagac ggatacagtg agtgaagtag cttgctacga acgaacaggc agaatgaaac
    11401 ggtagtttca ttagagcaag tcgcttgcga cgtagcgtta ccactgatgt aactggcaag
    11461 ttacatagag agagtttgct tgcaaacgaa cgttacccac ttgaatagaa aacaaaaata
    11521 acatattagt gaattttgaa taggagccac aactcaacca accgcttgcg gtgggtgagt
    11581 cccacggagg aaaagacgaa cgaacggtcg cgtagc
//
LOCUS       DACDCJ010000083        11587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDCJ010000083 DACDCJ010000000
VERSION     DACDCJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.083"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(118..1248)
                     /locus_tag="IV038_002337"
     CDS             complement(118..1248)
                     /locus_tag="IV038_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP8342523.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1789..1995)
                     /locus_tag="IV038_002338"
     CDS             complement(1789..1995)
                     /locus_tag="IV038_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342524.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2011..2625)
                     /locus_tag="IV038_002339"
     CDS             complement(2011..2625)
                     /locus_tag="IV038_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP8342525.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2772..3455)
                     /locus_tag="IV038_002340"
     CDS             complement(2772..3455)
                     /locus_tag="IV038_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8342526.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3564..4022)
                     /locus_tag="IV038_002341"
     CDS             complement(3564..4022)
                     /locus_tag="IV038_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP8342527.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4106..5869)
                     /locus_tag="IV038_002342"
     CDS             complement(4106..5869)
                     /locus_tag="IV038_002342"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP8342528.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6090..7550)
                     /locus_tag="IV038_002343"
     CDS             complement(6090..7550)
                     /locus_tag="IV038_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342529.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7758..8684)
                     /gene="glsA"
                     /locus_tag="IV038_002344"
     CDS             complement(7758..8684)
                     /gene="glsA"
                     /locus_tag="IV038_002344"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP8342530.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8707..10134)
                     /locus_tag="IV038_002345"
     CDS             complement(8707..10134)
                     /locus_tag="IV038_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP8342531.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10165..11379)
                     /locus_tag="IV038_002346"
     CDS             complement(10165..11379)
                     /locus_tag="IV038_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP8342532.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3507 a         2403 c         1912 g         3765 t
ORIGIN      
        1 aatatgattt tttgagatac tttcattgcg aattcctctt tctaaaagaa cctctcgctt
       61 gaaaaataaa agattgtact ttatgataat tcaaactgag agtttctcag tttctcttta
      121 ttgtttttca ggtcgataga gcgccaactc ttcggcctgt atctttgaag aaatattcaa
      181 cgtgattccg attcctaaag aaaggatcag ataacttgtt cctccgtagc taacaaatgg
      241 caatggcaca ccagtcattg gcatcagccc cgcgatactt cccacgttca ttatcgtctg
      301 aacgaatagc aaagttccga atcctaaaag aaacaatcca gcttgctgat ttttacagcg
      361 actgcttaaa caaaagattc tcatgcataa tgagaacaat aagaaaagca cacacaatgc
      421 accgattagc ccaagctctt ctgtaataat cgaaaaaata aaatctgttt ctgtctctgg
      481 aagataccct ttttttgtga tactatttcc taatccccgc ccaaatatgc caccattata
      541 tagtgcataa aacgaatggg tcatttgata gcccgcacca tgactatcga taaaagaatc
      601 tcttaaagtc gcgatccttt cataagcatg attaaacatc tgtggaagcc aacctttatt
      661 tcctaaatat aataccccgc ctgctgctcc aatcagcaat gcagaaaaag taacgattag
      721 atagatccct tttttaaatg gaaccgccgc tgcccaaaat atgacccacg caatcgaaag
      781 gatcatcaat gctccagcaa tctttggctg aaataaaatc agaaaagtta tacctactgt
      841 aatcaggaat ggttttttta gatcttttgg gctactattt ccgtcacgaa aaaaccagct
      901 taaataaaaa atcaaaaaaa gatttgccag ttcagaaggc tggaattgaa tgccaaacag
      961 agagatccaa cgttgtgcgc cgttgacagt gacaccgaat atccctattc ttactaatac
     1021 taaaaagaaa atcgataagg ctaaaccgta tcctgctatt ttagggtgaa gtaaatagtg
     1081 tagtttgact gaacgagcta agaagatcac gctccaactc aaaaatatga aaattagttg
     1141 acgcaataat agactttttg tattttcatc ggcttgcaat aaacgataag aactggcact
     1201 gtatacttct aaaagaccga tcatggacaa agtcaaataa ggacccaaaa tccaccaatc
     1261 gatctttttt caccgcatct cctcctcaca cagcaatcca cttgtcaggt gaggagcgag
     1321 tttgtctcat caaataagtc agtcacaaaa atataaagaa aaggtcagaa accttttcga
     1381 acaagaattc tgacctagca tcatctcatc gtccgagttt tagcactata caacgtaaag
     1441 aaaacattct tagctattgg aaaaagcctt aatccaacag tttctgtttt acccattggc
     1501 gactttcgac gtttctgggc aacagcctat gtttgtatag gagcctcacc taacaaggtt
     1561 attactatat tctatttacg agttcatgat agacttttcc taatgattag gcaatggaat
     1621 ttgaaatgaa atagtgaaat ttaacaatct ttaatatttt ttagaaaaaa ataacaaata
     1681 agttgaaaga atcccgttgc aaattctttc agttgcacca ggattctttg aaaatttttt
     1741 agttttatgt gtttttttat atgttttttc acttttcgga ataaatattt atttgttaaa
     1801 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
     1861 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
     1921 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
     1981 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
     2041 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
     2101 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
     2161 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
     2221 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
     2281 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
     2341 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
     2401 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
     2461 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
     2521 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
     2581 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
     2641 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
     2701 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
     2761 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     2821 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     2881 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     2941 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     3001 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     3061 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     3121 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     3181 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     3241 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     3301 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     3361 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     3421 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     3481 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     3541 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     3601 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     3661 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     3721 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     3781 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     3841 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     3901 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     3961 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     4021 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     4081 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     4141 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     4201 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     4261 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     4321 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     4381 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     4441 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     4501 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     4561 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     4621 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     4681 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     4741 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     4801 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     4861 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     4921 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     4981 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     5041 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     5101 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     5161 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     5221 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     5281 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     5341 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     5401 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     5461 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     5521 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     5581 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     5641 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     5701 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     5761 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     5821 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     5881 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     5941 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     6001 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     6061 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     6121 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     6181 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     6241 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     6301 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     6361 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     6421 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     6481 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     6541 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     6601 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     6661 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     6721 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     6781 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     6841 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     6901 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     6961 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     7021 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     7081 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     7141 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     7201 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     7261 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     7321 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     7381 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     7441 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     7501 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     7561 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     7621 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     7681 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     7741 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     7801 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     7861 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     7921 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     7981 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     8041 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     8101 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     8161 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     8221 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     8281 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     8341 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     8401 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     8461 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     8521 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     8581 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     8641 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     8701 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     8761 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     8821 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     8881 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     8941 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     9001 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     9061 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     9121 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     9181 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     9241 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     9301 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     9361 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     9421 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     9481 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     9541 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     9601 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     9661 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     9721 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     9781 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     9841 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     9901 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     9961 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
    10021 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
    10081 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
    10141 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
    10201 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
    10261 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
    10321 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
    10381 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
    10441 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
    10501 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
    10561 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
    10621 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
    10681 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
    10741 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
    10801 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
    10861 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
    10921 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
    10981 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
    11041 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
    11101 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
    11161 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
    11221 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
    11281 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
    11341 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
    11401 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
    11461 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
    11521 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
    11581 ctttttt
//
LOCUS       DACDCJ010000084        11504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDCJ010000084 DACDCJ010000000
VERSION     DACDCJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.084"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..2128)
                     /locus_tag="IV038_002347"
     CDS             complement(53..2128)
                     /locus_tag="IV038_002347"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP8342533.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2130..3047)
                     /gene="glyQ"
                     /locus_tag="IV038_002348"
     CDS             complement(2130..3047)
                     /gene="glyQ"
                     /locus_tag="IV038_002348"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP8342534.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3431..4243)
                     /gene="recO"
                     /locus_tag="IV038_002349"
     CDS             complement(3431..4243)
                     /gene="recO"
                     /locus_tag="IV038_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP8342535.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4386..5285)
                     /locus_tag="IV038_002350"
     CDS             complement(4386..5285)
                     /locus_tag="IV038_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP8342536.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5300..5701)
                     /locus_tag="IV038_002351"
     CDS             complement(5300..5701)
                     /locus_tag="IV038_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP8342537.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5679..6155)
                     /gene="ybeY"
                     /locus_tag="IV038_002352"
     CDS             complement(5679..6155)
                     /gene="ybeY"
                     /locus_tag="IV038_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP8342538.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6172..8364)
                     /locus_tag="IV038_002353"
     CDS             complement(6172..8364)
                     /locus_tag="IV038_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP8342539.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8388..9359)
                     /locus_tag="IV038_002354"
     CDS             complement(8388..9359)
                     /locus_tag="IV038_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP8342540.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10065..11444
                     /gene="brnQ"
                     /locus_tag="IV038_002355"
     CDS             10065..11444
                     /gene="brnQ"
                     /locus_tag="IV038_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP8342541.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3105 a         2422 c         2120 g         3857 t
ORIGIN      
        1 aaacagagac acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg
       61 tgatgatttg atccaagctt gctaatgaca aagccatggc agttatttga ctaagttgtg
      121 ttagtcggtt cttacgtact gctggttcat cagccatgac catcgtttga tcaaaataat
      181 tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag
      241 caaaacttgc tttcattgct tctactgctt tgtgtaattc tttctctgcg tcgttttcga
      301 acagctcagg gtctacttct gattgttttt caagctgcgc ttttttcgct aagttcacga
      361 cacgtgtcaa tgcttccatt gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca
      421 attgtgctgc tgcaaatact tttgtcagat ctttttgttc tgcattcaaa acagcttcga
      481 tgacatcatg acggatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt
      541 cgatcacctg ttgttgtccc gctgacaagc caatgccaaa acgatccgta tctttactga
      601 taacttcact aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga
      661 tgattcgaac gacaccatat gcttgacggc gcaaagcata tggatcgttt gagccacttg
      721 ggatcatccc aactgcaaag aaagaaaaga tactatctaa tttatcagca atcgctaaaa
      781 cagaacctac tgtagtctca ggtaaatctc cttcactgga tataggcatg taatgttcac
      841 ggatcgctgt tgctacggct ggtttctcgc ctttaagcag agcatatttt tctcccatga
      901 tcccttgtag ttcagggaat tctcctacca tgttggttac tagatcaaat ttatagattt
      961 cagcagcgcg ttgcagatcc atcaattctt cctcagatag tcctacttgt tcgcctatga
     1021 tgcgagcgat gacttgtacc cgttgcattt tctcatagat gctaccgatt ttttcatgga
     1081 aagtaacagt ttttaatttt tcaacgtatt ggtcgattga agctttcttg tcttcattat
     1141 aaaagaattc cgcatcttct aaacgagcag tcaatacttt ttgattgcct cgtaccacat
     1201 tttccaattt tacccgatcg ccattacgag cagaaataaa atgaggaagc aacatacctt
     1261 gatcgttccg tacttcgaaa taacgttgat gttctttcat agaagtgact agtacttctt
     1321 caggaacaga tagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta
     1381 gattgttgac ttcttctagt aaatcttcat ctagatcaat gatccagtga tttttttctg
     1441 ctaattcgtt tgcttgtgtg atgatcattt cttttctttt ttgcggtgaa gcaataacaa
     1501 attgttcatt caatttttct tcgtattctt ttgcatgcgt aaatgtcaca tcttcgccta
     1561 agaaacgatg acctcttgaa actctgtctg ttttgacatc caagatctca aacgggatta
     1621 tttcgtcatc taacaatgcc acgatccagt gaatagggcg aatatattca aaatcgtatt
     1681 ctgcccagtg catcgttact gggaaagtga ggcttttaat gacatctgct aaacctgtca
     1741 atacttcttt tgcttctttg ccgtgagtgt atttcgttac atagacatat tccacgcctt
     1801 ttaattctct aaaagtgata gcatctgtcg tcagaccttg tccgcggaca aatccttcag
     1861 ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt
     1921 cttcttcgct gtctgcttga cgttctggaa tgtttgttac tttgattgct aagcggcgcg
     1981 gtgtagaaaa cgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg
     2041 ttttttcttc taattgtttc atgcttggtg tgactacatg agcaggcatt tcttccagcc
     2101 cgatttcaag taataagtct tttgccatct tattcagcct ccttcacgtt ttctggattt
     2161 gctgtgtttt tattcaataa agggaatcct aatttttcgc gttctgccac aaagattttg
     2221 gctacagcac gagccatgtt gcggatacgg gaaagataac ctgcacgctc tgttacggat
     2281 actgcaccgc gagcatctaa taaattgaat gtatggctac atttcaaaat ataatcatac
     2341 gctggatgaa caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaaa
     2401 ttgcttagta gcaggtcttg gtcgcttact tcaaaagaat atttagaatg ttcatattct
     2461 ggttgtttga agatctcacc atatttcacg ccatcagccc attccagatc ataaacactt
     2521 tcaacttctt ggatataaga agcaagacgt tctagtccat aagtgatctc agaagtaact
     2581 ggtttacaag gtagtcctcc gacttgctgg aagtacgtga attgtgtgat ctccatccca
     2641 tcaagccata cttcccagcc cacaccggca caacccatcg atgggttctc ccagttgtct
     2701 tcaacaaagc gaatatcgtg ttctaatgga tcaattccta aaagacgcaa gctttctaaa
     2761 taaagctctt ggatattttc aggtgaaggt ttcattacga cttgaaattg atgatgctga
     2821 tacaaacggt ttgggttttc tccataacgc ccgtcagctg gtcggcggga tggctctaca
     2881 taagcagcat tccatggttc cggtccgatt gcccgtaaga atgtatatgg gctcatcgta
     2941 cctgctcctt tttctgtatc ataagcttgc atcagcatgc agccgtttga tgaccaaaat
     3001 ttttgtaatg ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct
     3061 ttcaattttt aagcgttgct gtcaataaga atacaggaaa gaaaacgaca aaaagtccct
     3121 atgtctgatt acagacatag ggacgaataa tttcgcggtt ccaccctact tccgaataaa
     3181 gattcggcag cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg
     3241 cttccactct cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt
     3301 attcattttg cagttttctg gtttgtataa tagatcctct cagtgaacga tctatttaat
     3361 ctacggttaa taatagacgg aagtccctgt atttgtcaaa tagttttgat ttttttgttg
     3421 tatctttcat ttattcttct ttttcaggta tctctttttc aggcatattt ttctctggcg
     3481 tctttaatac gtgtgcccaa gacttcattt gatcaatgaa ctttttgctt tttagatgaa
     3541 taccgacata ttcatcatac aactggtcaa tgaggtcacg gattgcctgc ttcgtttctt
     3601 ctttcaagtg gattccactg atcttatcat aagaaatagc cgaaaacatt cgaacgaaat
     3661 aaacagcgcg aggatctgca tgataacggt gttcgtccat tggccagtgt ttctcgcata
     3721 tcacaccgcc atatttggaa gaatagtcaa attttccttt tgtttccccg catacgacac
     3781 agtgcgtcca aattggcgcg attccgaagc gttgcagtat ttgcacttca aagatattcg
     3841 tgatgatttc tgcatcatct ccttggtcca tccgctgcag agcttgttgc gtaaattgga
     3901 ataaatgcgg gtcatattga tgatcttcga ttgctgcatc tactagattt agcaaatacg
     3961 taccataacc gctcaaaaag atatcttctt ggatgttccg aaacgcatgg acgtctttga
     4021 cactgcttaa aaacgacaaa ccttcacttc gaaaattacc gatatagact gcttcggtaa
     4081 aggggagcaa agccggagct aatggctgat tttgccgatg gattcctttg atataaaaca
     4141 tcattttgcc agaagattcc gtaaagattt tgactaattt atctttttct ttgtaattac
     4201 ggctagataa aatcaagcct ttcgattcta caagctggct catattctcc ctcctaaaaa
     4261 ataaatctgg aaaagcagat tctgccgatc cagattattt atgtttcgat cttccgctgt
     4321 ttttctcaac gtacggattt ttatccagtt tatttctata cctaacctat aagaagtcca
     4381 gaatatcagt actcgtcttt tcggtagccg aagtcttgca gatagacttt tttgtctcgc
     4441 cagtcttttt ggactttgac ccaaagttct agaaatactt tgtctcctaa aagattttcg
     4501 atatcctttc ttgctttcgt tcctacttgc ttcagcattt ttccgccttt gccgataatg
     4561 attccttttt ggctatctct ttcgacgatg atcgtggcct ggatatggat tttatcattc
     4621 tcgtttcgct tcatcgaatc aacaacgact gcgacagaat gaggcacttc gtctcgtgtt
     4681 aaaaacaaga ctttctcacg gatcaattca gaaacgatga agtattcagg atgatcagtc
     4741 acttgatcat ctggaaaata ttgtgggccc tctggcattt cactgactaa tacatccatt
     4801 aagcgttcga cattgtttcc ttctgtagca gagatcggaa tcacttgtgc aaaaggcatc
     4861 tgcgtcgaat aatcttcgat gattcccagc aactcgtctg gatggatctt atcgatttta
     4921 ttgataatca gataaacagg agaatgcacg ttttttagac gttcaataat aaaatcgtct
     4981 ccccggccac gtttttgatc agcactgatc ataaataaga ctgcatccac ttctttcaac
     5041 gcactgtaag ctgcttctac cataaaatct cctagacgat gctttggttt atggattcct
     5101 ggtgtatcga taaaaatcag ctgagcttct ggtgtagtat atactccttg gattttattt
     5161 ctagttgtct gtgccttgtc actcataatg gcaatttttt gacccacgat tcggttcaat
     5221 aatgtggatt ttcctacatt cgggcggccg acgattgcga caaaccctga tttatgttct
     5281 ggcatatacg ttctcctttt taattaaaca acaaatgata tattttaggg acaaataaaa
     5341 tcaacccaat gatgacagca aaacaagcgg tcactaaaac tgctccagct gccatatctt
     5401 tgattttttt cccaatcgga tgaaaatgaa aatccgtaaa catgtccact acattttcaa
     5461 aaacagtatt gatgatctct actatccaaa cgagaaaaac agctaaaagc agccataacc
     5521 actctatccg atcaagctgg aaaatcacgc ccataaataa agcaaggata ccgaacgcaa
     5581 catgtttcct catattgcgt tcttcctcaa aaacggtttt gactccttgg acagcaaatt
     5641 ccacagacat caggaaatgt ttgtttttct ctacgttttt atcttttgag tccataggca
     5701 tccaagatct ctttctgtaa accaaacatt tctttctcat cttctggtgt catatggtca
     5761 tacccgttga tatgcaaaaa tccatgtacc gctaaaaagc caagttcccg atcatatgaa
     5821 tggccgtaag cttctgcttg ttcagcagca cgttcaatag agatcatgat gtctcctagt
     5881 acacgaggta atggattctc tccatcttca aaaatcactg ggatttcgtc ttcgccttct
     5941 tcttccagag caaaactgat gacgtctgtc ggtgcatctt ttccacggta atcacgattg
     6001 attacttgaa ttgccgcatt gtccatgaaa gtaacagaca tttctgtttc ctctggcagc
     6061 tttaaataag atgctgcaaa ttgcagcaag tcatcgattt ctttcatttt ctcttctgag
     6121 acttggtttg tttcatccat aaaggtaata tccattatac atcgcttctt tctattcttc
     6181 ttgtacttcc tttatcttct caacttctgt tttcatttta ggatatttga tccgagaatg
     6241 gaaggtgcta cttaaaccgc tgattaatga tttttccact tttcggattt ctttcaaggt
     6301 cagtccacta tcgtctaatt gaccatcgga aatacgctct tcgataagat tatggacaaa
     6361 actttcgatt ttttcgctcg tcggatgatc cattgcccgt actgcagcct cacaactgtc
     6421 agcaatattg acgatccctg cttcacgact ttgcggtttt ggtccaggat aacggaattg
     6481 ctcttctgtt acttccggat tgcgttcttt cgctttgatg tagaaataac gcattagcgt
     6541 ggttccatga tgctgtttgc aaatatcgat caccatttgc ggcatattat aatcttccaa
     6601 tattttggct ccatctgtca catgtccaaa gataatatgt ttactgtctt ctggcaataa
     6661 gaaattgtgc gggttttccg ctcctgaagg caagttttca acgaaaaagc tagcatgttt
     6721 gatcttgccg atatcatgat aatagcaagc tactcgagtt agcaaagaac gtccgccgat
     6781 ttctgctaca gcgttagcac ttagattagc taccatcata ctatgatgat aagtacctgg
     6841 cgcttcttcc agcaactgtt tcaataaagg atgatttgga ttgctgagtt catttaatac
     6901 aattacactg tcatcattga ccatcaattc aatatatggg tgtagaccga tcgtcagcaa
     6961 gaatgagaag agtgcaccag tgaacccgca gacgagcatt aaccaagtcg ttgcatcacc
     7021 aaagctcatt ccttgataga cgatcaaaat gacatccatc atcacaggaa aaataatgac
     7081 ccacatggca gcagataatc cttgttctga gatccgtttg cgtctgacaa tcgttgccat
     7141 gagcccagag aaaacataag acatcaagat aatcgttaga aagttcgtgc caatcgaatc
     7201 ataaaaaatg aaaagtgcgg atactgcttg gaaaatacca gccatgatcc ctgctcttcg
     7261 attcaagaaa aagcttaaaa caagtggtgc aaaagcagcg ggataaaaca gcgggatata
     7321 agtagcttgt tccgtttgga ataattggaa aaatttcatc agcactatgc tgactgacat
     7381 agctgtcaca tagaaaagca cgtattttgt tctttctcct gcattttgga attgaagtga
     7441 attataaatc agtacgccga tttgaagcaa aacagctaaa accatggcaa ttagcggaaa
     7501 aatcgaagtt gtttgattag tcagtcctag taattccaat tttttcatag ctgaagcatc
     7561 gatttgatta ccttcacgaa caatgatttc tccttggaag atcatcactg gttgtacagc
     7621 ttctcgtgcg gattgtttca attcttctgt ctttttctca ttcaatacgt cattcactac
     7681 gataccttga tccactaagt agcgaatcat ttgttgttgt gctggtgtga cattcaaata
     7741 ttggatttgc tcttctgctt cttgtttaaa agcttccaat tcactttcac gaacttgttt
     7801 gctcatctgt tcgtcaatga gcttcagact ctcatccctc acagtatgaa tctcagtttc
     7861 agtcatttca aatatcgtcg aataaaaatt gttcggtagt ttttgataaa acacaacatt
     7921 ttctgcattc acattttcaa aatttttctt cagcgcggcg atccgctctt ctgtagtagg
     7981 ctttgggata ttttcatctt cctttgcctt atcaatcttt tcgtcataag atttgttgat
     8041 ggcatcattc gtcttatcga tcaattcaaa tagttgcttg atccgattgt tctggtcatc
     8101 tgcaagatct ttttggtaag tgtattctgg agtgactgct tctgctgcca gttttctttt
     8161 ttgttcggtt tcttccgtgt tctcgatcgt tttgttcgca cggatacttt cttctgctac
     8221 ttggccttct ctgaaatcat tggccttttg tttgacacta ctaaatgtaa cgccgcagat
     8281 aatgataaaa aacaagaaca gcaggaaagg caatgctgcc tttccaagtt tttcttgtag
     8341 tttctgcagt gttttattca acatgtgctt atcctcctaa gcaaagctta ttcttgatgg
     8401 agatctgcct tttcataagc tttgatgatt tcggcaacta cagggtgccg aacaacgtca
     8461 cttgcttcaa aattaacaaa atcgattttc ttgatttgct ttaatgttcg ttctgcatga
     8521 acaagaccac ttgtcgtccc tcgaggcaag tctatctgac ttgtatcccc attgacaatc
     8581 atttttgatt gataaccgag acgggtcaag aacattttca tctgagcgac tgtcgtgttt
     8641 tgtgcttcgt ccaagataac gaatgcatca tccaatgttc gcccccgcat gtaagctaaa
     8701 ggtgcgattt cgattacgcc tcgttccatc aatcggttcg tatgatccaa tccaaaaatt
     8761 tggtataaag catcataaac aggccgcaga taaggatcca ctttttcttt tagatccccg
     8821 ggaagaaagc ctagattttc acctgcttcc acagcaggcc gcgtcaaaat gattttttgc
     8881 acttcacctt ttttcaaggc agcgattgct aatactactg caagaaaagt ttttcctgtc
     8941 ccggctggtc ctacgccaaa tacgatatca tgtttggcta ccgattcgac atatttcttt
     9001 tggccgctgt tttttacccg tataggtttt ccattacggt ctttgacgat ctcttcctca
     9061 tacatctcaa caaaataatc taatgtacct ttttgcccca ttttcaatgc cgtcacaaca
     9121 tcgggcgtac ttacgtggat tcctcgtttg atcaattcct gtaatgtccg aatgactgag
     9181 gcagccaagg aaatctggga ttgctcacca atgatctgaa tgatttctcc acgtgtatgg
     9241 atggttgttt ccgttgtttc ctcaatcagt tttatatgct tgtcgtgtgc acctaaaagc
     9301 atgctgacat catcagcagc cgtcaatcta atctctaaag aactagactc ttccgtcaaa
     9361 aatagacatc cccttttttt cgattaattc tttcctattt taccatactc agaagaaact
     9421 atatagcaaa taagaaaagg ttctaaaaga gttgcttaac cataagtacc aagaaacgtt
     9481 aaaaaactag ccgcttccat tctctaaagg tctcgtttga ataaaaaaag ctgatcatgt
     9541 aagctatgca taagatagag gacaagtatt ccgtctaact tagcaagtat tctctgggta
     9601 tttttcttgt ttcaaaaaaa gaaaacaaaa tatttgaaat attcttgcat ttcaaaaaaa
     9661 acaggtgtat tataacgtca acaaataaat atacagcaaa gcgtcgagga aaacagtagg
     9721 ctattcagtg atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag
     9781 acgaacatca tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc
     9841 aggctagcgg ataactttat cgaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa
     9901 aaaggtggta ccgttctaga atctagagcc cttttcccaa gtggaaaagg gcttttttta
     9961 tattttaagg tggtggtttt catggaatct gatattagtg gtatttctag tttctgcaac
    10021 gtttattttg ttactcaaaa tgataattat tggaggaaaa atttatggaa gaaacaaaaa
    10081 cttattcatg gaagcaatta ttgatggtca gttcacttat cttcgggatg ttcttcgggt
    10141 caggtaatct gatctttcct gtacacttag gtcaaatggc tggatcaaac tggatcagcg
    10201 ctggtgtagg atttgccatc tcaggcgctc ttttcccatt attagctatc ttagctgtag
    10261 tagtgacaaa aagtgatggt ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg
    10321 ccttgttctt gatccttgtt catttaacga tcggaccatt ctttggtaca ccgcggacag
    10381 cagcaactgc ttttgaaatg gctgctaaac cctttttgcc tgaaaaatat gattcattag
    10441 gtatgttttt attctctgct cttttctttt tacttgctta tttccttact gtacatccaa
    10501 cacgtttggt caaatatgtc ggaaagtatt taaactcctt gtttcttgca ttattagcta
    10561 tcatttttgt cgtttcttta ttccaaccca tggggaatct aaaccaatct gctgcagatt
    10621 cttatcagac gaacgctttg acaaatggcg ttttagaagg atacaacacg gttgatgcag
    10681 tagctttact tgctttaagt gtcacttttg tccatgcagt tcgtgatctt ggttttaaag
    10741 ataaaaaagt cacagaatta acagcaaaag ctggtagcct gagcgttgcg ttagaagtca
    10801 tgatttattt tggtctggtc cttctcggtg cgatgagttt gaataagatg tctttatcag
    10861 aaaatggcgg aactgcactt tctcagatca ctaattttta tttaggtaac ttcggtgcta
    10921 gcttcttagg ggtaatggtc acattaggtg tcttcactac tgctatggga ctcgttgttt
    10981 cttttgcaca agatttccat aaactgttcc ctaaagtcag ttatatgaca tggctgcggt
    11041 taacgacttt tgtctctttt gtcgttgcga atgccggatt ggataatatc atccaatggt
    11101 ctttaccagt attgatgctt ttgtatccat tgtcacttgc gttaatctta ctttccttaa
    11161 cagcaaaatt cttccaaaag agtccatttg tctatcaagt aacgatgttc ttttctgccg
    11221 ttccagccgt attggatatg ttggcaaatt cgcctgcatt agtgagtcag caaactgtcg
    11281 tagcaagctt actaggattt tatcatcatt atgttccttt tgctgcatta ggtcttggct
    11341 gggtcgtccc aacattgctt ggttacgctg gcagcttgct cttttattat gtatatcgcg
    11401 tatcaggata taaacaagaa gcaagtgagt taccagaaga ataaccaaat caaacacaat
    11461 ttaacataag agatattaaa caggaaaaaa acagctagaa gaaa
//
LOCUS       DACDCJ010000085        11378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDCJ010000085 DACDCJ010000000
VERSION     DACDCJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.085"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1056
                     /locus_tag="IV038_002356"
     CDS             <1..1056
                     /locus_tag="IV038_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP8342542.1"
                     /translation="AIDNMDGIMTVCLGRNMLFDLVIQSYSQLETRYDKAFKTIKENC
                     QNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVERAS
                     QLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDI
                     ACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEE
                     ELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKEAIELVKVINGRLADENSADMV
                     IKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMILEQQLANAYELAVFKTKNSILN
                     NKIIKLKNAMTKLAKAI"
     gene            1107..3758
                     /locus_tag="IV038_002357"
     CDS             1107..3758
                     /locus_tag="IV038_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342543.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTLIGSTEK
                     LSGIMFAKTLYNDATSINNQDGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNAL
                     KEKNFFSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFS
                     DSFKGGYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIE
                     TGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVF
                     VLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSDS
                     GEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMR
                     DMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAKV
                     ENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELIN
                     QKRQEAKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGLEKLVKE
                     NLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIGVRENLQS
                     SSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQG
                     FKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKP
                     RRRFTYEDNELFRDTLNDPNPLFDNLN"
     gene            3782..4117
                     /locus_tag="IV038_002358"
     CDS             3782..4117
                     /locus_tag="IV038_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342544.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            4114..4731
                     /locus_tag="IV038_002359"
     CDS             4114..4731
                     /locus_tag="IV038_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342545.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            4748..5215
                     /locus_tag="IV038_002360"
     CDS             4748..5215
                     /locus_tag="IV038_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342546.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            5231..6808
                     /locus_tag="IV038_002361"
                     /pseudo
     CDS             5231..6808
                     /locus_tag="IV038_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
     gene            7544..8584
                     /locus_tag="IV038_002362"
     CDS             7544..8584
                     /locus_tag="IV038_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342547.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEDCSVAMRKIARELRPSFEIRLR
                     EQGISKEGQYGKYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEG
                     REAIHKKQQTVEEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILS
                     EMHCHHIIPYHESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLN
                     ELRLKVGNEEIS"
     gene            8718..9092
                     /locus_tag="IV038_002363"
                     /pseudo
     CDS             8718..9092
                     /locus_tag="IV038_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
     gene            9114..10250
                     /locus_tag="IV038_002364"
     CDS             9114..10250
                     /locus_tag="IV038_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342548.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            10265..10915
                     /locus_tag="IV038_002365"
     CDS             10265..10915
                     /locus_tag="IV038_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP8342549.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            10929..>11378
                     /locus_tag="IV038_002366"
     CDS             10929..>11378
                     /locus_tag="IV038_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342550.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYKNLPKDF"
BASE COUNT         4298 a         1511 c         2333 g         3236 t
ORIGIN      
        1 gctatagaca atatggatgg gattatgacg gtatgtctag gaagaaatat gctttttgac
       61 ttagtgattc aatcctatag tcagctagaa acaagatatg ataaagcttt taaaacaatt
      121 aaagaaaatt gtcagaatca tattttaatc atgtcgaacg atgaggaaac gatagaagag
      181 atttcaaaaa aatgtggtca taagacaaca ataaatagaa gtgctagttc taaacattta
      241 gatactgata gctcggtcac tagttcggca gagcaagagc gagtaattac agttgagcgt
      301 gctagtcaat tgattgaagg ggaacaaatt attttaagaa accttcatag acaagataca
      361 aaaagaaata agattagacc atttcctatt tttaatacta aagaaacaaa tatgccgtat
      421 agataccaat ttttagcaga agactttgat actcaaagag atattaatga gattgatatt
      481 gcatgtgaac atatcaatct atctttacaa gataaccaaa ttccatatgc aaggtttatc
      541 aaagatttaa aaacacgttt ggagtattca attattaata atattcctat tagtgaagaa
      601 gactatcagg attatctaaa tttatcagga tcacaacaaa caattaatga agaagagctg
      661 ttaaaactgg taaatacgga agtaaaaagt caagatatgg aaggtcaagt tttatcagaa
      721 caagaaatga aaattaatat ttatctaaag gaaagtcaaa aggaagcaat agaattagta
      781 aaagtaatta atggaaggtt agctgatgaa aattcagcag atatggttat aaaaaccatt
      841 aattcacaaa tccttgatac gcctttcatt caatctttaa tcaatgattc tatagctatt
      901 cataatgttc aagagagtaa ggatcaaatt atgatcctag aacaacaatt agctaatgct
      961 tatgagttag cagtgtttaa aacgaaaaat tcaatactga ataataaaat tatcaagttg
     1021 aaaaatgcta tgactaagct agcaaaagca atttagagag aagggaagaa atattgactg
     1081 aatctattaa ttggagaaaa ctaaatgtgc aatggttaaa gtataaatgg ccaatcatag
     1141 tttccctaat aatcattcta tttatggcct atccgcaacg agtttttgcg ttgccgtgga
     1201 ttacagacaa taaagaggta ttaaaatttt taacaaaata tcaagattat cttcagttta
     1261 ctaaaggtgg ttttggaagc attgtcgttc aaagtttatc gtgggggctg attagaggtg
     1321 catattttgt ttgtaaatca gcagaaaacc ttgttccaca atcgctaagt ttgtttagct
     1381 ttgttgaaag cagtagttta ggtgctgttt atcaatctgt attaaacacg gtggttgtag
     1441 gattaatgat cctttcattg atgtttgttg gttacaagat gattactgga aaaggaacag
     1501 tggatttaaa atcggttgga atgaacgtag tgatgtctat agcattaata cttttaatgc
     1561 cgactctgat tggatcaaca gaaaaacttt caggaataat gtttgcaaag actctttata
     1621 atgacgcaac tagtatcaat aatcaagatg gcggtgtagc atggtcattg attgaggacg
     1681 gtgtaactga tttagtatat atcaataaaa acgatgctta tgatcggttg ggtaaagatg
     1741 gcgtacaaaa aaatgcgtta aaagaaaaga atttcttttc ctacgatttc agtgaagtaa
     1801 tcacagttga tacagctaaa gatttagaag atgataataa aaaagctgaa tatcttcaat
     1861 ctaggttgac accagatggc gaaggaaata tgcaagccgt taagattgat gatggttgga
     1921 tgtcgatgtt ctctgatagt ttcaaaggag ggtactacag gtttactaaa aatacttttg
     1981 cattgacttt aggattactt tcgttagctg tagcctatgt ttttagtatt tttatcatag
     2041 tcaccgctat tttagaactg gcatttaagc gtgtgttagg ggttcttgtt tttgcaacag
     2101 atattgagac aggacagcgt tcaaaagttg ttctatcaga tattctacag tgctatttga
     2161 ctgtaggctt tcaaggattt ggattatcca tgtttgcaat gtttgtttcc tatttaggaa
     2221 caggcggagg gtcttccgtt aatccgttta ttaaggctgt agcttatgta tgtgccgttt
     2281 ttgttttaat caaaggcagt ggaacagtca tgagatactt tggtgttgat attggactca
     2341 aagaaggcta tgggcaactt gcttctgctt ttggaatggg tgcaatgctg tttagaaaag
     2401 gatcaaatgg atttaatcgt gccaaaggaa gcggaaacgg aaatggatca gacagcggag
     2461 aaggagaaga tcgaaaacca gaaaagaatt tcggtgaaac tctatcaaaa aaagctggca
     2521 aaactggaag agctttggga tatgcccatg aacgtggatt atcaggttta gcttcagatg
     2581 gagctacaat ggctagtgaa agagcaacaa aaccatttaa gagtatgcgt gatatggcta
     2641 atgatactaa gaataagttc aaagaaggtt tagatgatgg tacagtgtcc gctattaata
     2701 aaaatagcaa acctatgtta gctaaaaata aagaagatga aactggaaaa tatgctgatt
     2761 ccatgccaac acgtctttcg gatagaaaag atggtgctaa ggttgaaaat gccgatagaa
     2821 ttatgtctag ttctgaacgt atgcgtgaag caatgaaaaa taatgctgaa tcgaataata
     2881 atcctgtaag tgcaattcag caaaaagttc aacaagatat tgaagaacgt aaaaatgcta
     2941 tgcacggtca agctaagtct gctgaagaat taattaatca gaagcgacaa gaagctaaat
     3001 acacgcctga agcaatgaat cgtgaggaaa tgttaaggaa acgtgtagaa gggcgtacag
     3061 gaactaatac ggacgaaaaa gaagcgttga ccaaagaaag aatccaagaa gctaaaaact
     3121 caaataccgg attagaaaaa ttggtcaaag aaaatctaca aaattctaca agtagtcaag
     3181 gtggtcgatc agtagatgtc cgagaaaata ttcaaggttc attgaatggt catgtcggac
     3241 ggaatgttga tgtaagagag aatttacaaa gttcttcaaa tggtcaaggt actaagacaa
     3301 ttggtgttcg agaaaatctg caaagtagca gtacaagtca accaaaaaca gtagatgttc
     3361 gagaaaatgt acaaaattct acaagtagtc aagggtttaa atcagtggat gttcgagaga
     3421 atgtgcaagg tagcagtgca agtcaaccaa aaacagtaga tgtccgagaa aatgtacaaa
     3481 gttctacaag tagtcaagga ttcaaaacag tagatgttcg ggagaacgtt caaaaagata
     3541 gtggagaaat gaaagaaaaa acacaaaaaa tcaatatcgt tgaagacaga aagtcagcga
     3601 aaaaatttga ttcaaatcat gaaacagtga ttattgatag tgaaatccgt gaaaatgata
     3661 agggatcaaa accaagacga agattcactt atgaagacaa cgaattattc agagatacac
     3721 ttaatgatcc taatccactt ttcgataatt taaattaaat taaaagccgg catgagtgat
     3781 tatgccggct ttttatgagg tgaaaatgat gtatagaaat cccaaaaata taaaacaaga
     3841 gataaagcta tttttcttct atttagtcga tttaggaata gtagccggaa gcgtgatggc
     3901 aagtgtttac tttgcaaaat taatgccaat ttcaccaatg atgaaatttg cactgtactt
     3961 attagcattt gtagtgggtg tttggatgtg cgttagaacg cctaatcacc ctaaagagcg
     4021 aaatatttta atgctgtatt atctctttaa aatggataga aacagatacc acgaagtaga
     4081 gtttaaaaga aatagtaggg ggaaaattga attatgattg ttgctgaaag taatgaaaat
     4141 aaattaagtg gtgtttttga caaattctta gaactagtta ttccaaaaga taaagatatg
     4201 tgtgaacttt taccatataa aaaaatattg aaagacggaa cgttagtgac taatgacaat
     4261 aagtatcaag ccttttttaa agttaaaaca actgacttaa cttctatttc agaagatgga
     4321 tttatgcgta tggtaaacaa actcacttat ttaaatcgag tatatttaga accatataaa
     4381 atttattcat taacttatcc tactgaaaca gtaaatcaag cgatttattg gaagcataga
     4441 atacaggagg caaagagaag tttattgagt aataatgatc ccaatttaga aacgtatctg
     4501 aagtggaatc taaagctagc ttacgataat tatgaaagag ttgatctagt atcgacttta
     4561 ccagagttga tgttcgttat ttgtgtttat ggaaaaacac aaaaagaagt gaaaaatcat
     4621 gccgatgtaa tgaaacaggc cggtggtaga gaattagatt tacaaagaat tactgaaggg
     4681 gatttaatca atgtgataac taaattgatg aatcccaata cttctttata aggcggtgct
     4741 tattattatg ttaaagcact gtagaaaagg attttgtaaa gtaattaaag gtaaaaatgc
     4801 tgctgattta atctttgaat ggaaagaatc taatttaatt aatgtacgtg ggaaaaatgg
     4861 aagcggaaag acagaaagag tgatggctag tattgataat tcaaggccta gatcaagagc
     4921 attagtaata actgatgaaa aaaatcacta taaaggcata cgaacgatag aaaaaaaata
     4981 tttaactaac aatgatttaa aagtagtaga tgatatttta aatatttatc ctgattttaa
     5041 tttagttgtt tttgatgaaa caattcctgt gacagaaata aaaggattct atcaaagctt
     5101 ttccaatcta gcaaataaat atgaggaagt tctgtttgtt tggattacag aagaagatag
     5161 taatagcaag tcagcgttct ttagtgaaaa gaacgctttt ttacattcta tttaaaaagg
     5221 agaattgaac atgattgtaa gtaaaaaact agcaagaaat ctagtaagtg aagggtatga
     5281 cttaagatta gtagaaaagt atcaaccgag cggtggaatt aaatttgatg agcgttatat
     5341 ttctactggt gatggatatg taacttcgat tgctgttatt gagtttgcag aagatgtaaa
     5401 tagtcgatgg ttaacggata taatggcaat tccaaatact attgcaactt tcgatgtatg
     5461 tacggcggat aaagaagact tattacacaa aatcaatacg tctattggag aattagatta
     5521 ccgatcaaca aatgagaata gaacaactga caaaacagac gctttactag ataaatacaa
     5581 tttagaagat tttgcacaag aaattacgaa acgtggtgaa gttgtcaaaa ctgttcatgc
     5641 tcgtattttt gtttatagcg attctttaga agaattagag aaaagaatta gtgagattcg
     5701 taaagatttg gctagtgtca atcacaaggc aattgttccg caatttagaa tgaaggatga
     5761 atggatgtct ttatttcaag atggcatgga acaagaatca aaattatttg ccaagaaagg
     5821 cttaactgtt ccggcaaaaa atattggtgg cggtattcca tttcaccaca agagtttgaa
     5881 agatccacgt gggttttatt taggaactac gtcaactggt ggtgctttta tatttgatcc
     5941 attttattct acgaagcaaa gacggtcgtt taacggcttt attttaggaa agatgggttt
     6001 tggtaagtca actttaatta aacagctaca ggaaggccta caagcgaaaa attgttttat
     6061 tcgaggattt gacaagtcag gtgaatttga aggcatggtg aagaaagcag gcggtgtata
     6121 cgtcgattta ggcggtggtg atatggtagt aaatccattg caaatttatg caacagtcat
     6181 gaaaaaagat caatttggaa atgatgttgt agatgaatca aagagttttc aaaatcatat
     6241 cactaagtgt agtaatcaaa ttcgcttttt agaacctgaa attgatacgg caactgtacg
     6301 tgaatatact ttgatgttac aagactttta tattcattat ggaattttgc caaaagaatg
     6361 gagagaagcg agagacgaca acgaacgaaa tcaattaaat acaaatattt gtaacttgcc
     6421 taatgattca tatccaatct atggagagtt tcttaaattt attgaaaatc aacaattcaa
     6481 aattaaaaat gctactcctg aacgtgtccg ttctattgag ctaattaagc ttatcgttac
     6541 ggatatgtgt acaacttatc cgcaaatttt caacggagta acaacaatgc caaactttga
     6601 tacaacaaaa attgtttact ataacattga ttctattagt aaaatgagtg ccccagtatt
     6661 ccattgccaa ttatttacag cgttaacgac tatttggaac agtgccttgc aaaacggaaa
     6721 gattcaaaaa gaaaacttta aaaaatatgg cagtgattat tataagtatg cacaagattt
     6781 catggttttg atcgatgagt gtcacaatgg tgcgacctgt tctgcagtga aaagctgtgg
     6841 gttgcaagta gtctaaaaac tattgtaata actcttataa cgagaagtca aatgagtggg
     6901 taacgccaca aagggcttcc actgatactc cgacatgcaa caagtagttt atgcagacca
     6961 acgttgtgtg aagctcggtg aagtcggctg aaggataccg taaggtaaaa gaaatagtga
     7021 ataccacgaa agttctttag aggtaaaggg tgtatccgat aagtcgggtg tccaaaaaaa
     7081 cttaatatgg tgagtatgta caaacgtact gacgaactag cgaatgtact gtccgaaagg
     7141 catagctcct agaaatggga gagacagtga gcaaaaaatc actcattgtg ctaacttgtg
     7201 gttgagtaaa agtcgttgtt atgaaagacc atgctgcgtt acaggcgtag gagaagttag
     7261 ggggcaaaag ctagcatcta agttaaggca tgtggataga tataagggaa caggggaagc
     7321 catcaacatg gagagtgcat actcgatgaa gtggtagtaa gaaatatgtg aatgacaaat
     7381 aacttacttt agtgatggtg aaagtagagt cacgactgat gaaggttctg taatggaact
     7441 ggaggaacag gctcgattcg tctgtaaaag tcatgaaacc cgttcaacca tgggattctc
     7501 gtatgactaa aaaggtcacg agccaattgg agcgtgataa accatgacaa aagaaacaag
     7561 tccaaaagat aggaagttgc ggcatgcaga atattatggc atgacggaaa tcttcgataa
     7621 gatatactcc aaaagtttga agggagataa tttcaagaat ctaatgcaga ttatcatttc
     7681 tgataacaac attgtcctag catatcgtaa tatcaagaga aatcacggta gtgtgacaag
     7741 aggagaagat ggagtaacga ttaaagatat tgaaaatctc aaccgtaatg aattcgtaga
     7801 aattgttagg aagcgattcg cacattataa ccctcggaaa gtaaggcgtg tagaaattcc
     7861 taaatataat ggtaaattaa gaccgttggg tattccatca atatgggata gaatcgcgca
     7921 gcaatgcatc ttacaagtat tagaaccaat atgtgaagat tgtagtgtag caatgcgaaa
     7981 aattgcaaga gaactcagac caagttttga aatacgacta cgagaacaag gaatctcaaa
     8041 agaaggacaa tacggaaagt acgaaggata caagccttac ctacggtcag gacaaatacg
     8101 atatataaga gggtttccaa tattaccagt agggtatatt caacacagac atccgatgtg
     8161 taaaaaaaga aatattaata aatacaccga agaaggtagg gaagccattc ataagaagca
     8221 acaaaccgta gaagaatgga agatatcgtg gttgtgtagc aatccagtgc ttagtaagag
     8281 agcaacgatt gaatttgcgg acaatcgaat ttcaaaattc atcgctcaaa agggaagatg
     8341 tgcagtaaca ggtgaggagc ttatcttatc tgaaatgcat tgtcatcata ttatccctta
     8401 tcatgaaagc aaaagtgata gctatgaaaa cctagtgatt gttactgagg aggttcatcg
     8461 ggtaatacac gctacacaaa gtgaaacgat tgaggaactt ctaaaatatc taaaattaaa
     8521 tccaaaacaa aaagaaaaac tcaatgagtt gagattaaaa gttggtaacg aagaaatatc
     8581 ttaataaata tttgtaggac gggaaaacag aatgtctttt atagacgagg gaacgccgta
     8641 tgcggtgaaa gtcgcacgta cggtgtggac tgggggaaaa gacggagata atatcaaact
     8701 cttacctatc agtatttatc aattcaaata acaagtatgc ttgtgaatat gtactaagtt
     8761 ttgagcgaga aatgcgaaaa atgcgtgcag gtgtttattt tgcaactcaa acgcctgaag
     8821 aaattatccc taaagattct ggatcagaag tagcagatgt gattagaaac atttttaatc
     8881 tatgtacttt taaatgcttc tttaatttag attctgcatt gttggaagac ataaaaaaag
     8941 ttttaggaaa tacaattact gatacagaaa ttatgctttt accagaatta gaagtaggac
     9001 aagctgtagt tcaaacttcc agtgaagaca cctatatgat taattttgat ccatatccag
     9061 agcaaattga acgattcgat ggtggtcaat aatagaaatc ggaggtgaat ttaatggagc
     9121 agaacaaaaa taataatgtt gcttcaacac tcgctcgtca agggatgaag caagtatcaa
     9181 agaaaatagg tcttgcttta aaaataaaaa taggggtagt tataggactg ataacattgg
     9241 ttggcctttt agttataggt tctttagcta cattacaagg aacacaaact gcaactaata
     9301 caggaggtgc agaagtcatt ggtggcaaca aaccgttgtc acctgaagtt caaaaatggc
     9361 aaaaaacggt agaaaaatat gctgaaaaat acggaattaa agaattagta ccttatgctt
     9421 tagcaattat gcaatgtgaa tccggaggta aattgccgga tttaatgcaa agtagcgaat
     9481 cagccggatt gcctgtaaac acacttcaat atgaagaaag tatcgatcaa gggctaaaac
     9541 atttgagtaa tgtgtataac attgctaaaa gttatggcat ggaaagtaac tatgatggaa
     9601 tagttcaagc ttacaattac ggaatagctt acatgcacta cttaggaaaa aacaaaaaaa
     9661 cacatactat cgaaacggct gaagcctact ctaaaaatgt tgttgctcca agtttaggaa
     9721 atactacagg acagacttat tcatatgtaa atgaagtatc aattaaatat ggaaaaacat
     9781 atttgtattt aaatggtggt aatttttact atgcagagtt agttaaacag tatatcggtg
     9841 ggggtagtgg taatccggct aatccttcag aattttgggg aaaaatgaaa acagaaatgg
     9901 aaaaatatca aggttggcct tatgtttggg gcggtaaaaa tccaacgaca ggttttgatt
     9961 gtagcggatt aacagcttat ctttatcaat ttagcgccgg aattacaata gatagttata
    10021 cagtaacgca gtatgaagca actgaagcag tatcaattaa agaagcaaaa gctggtgatc
    10081 tgattttctt tagaggaaca tatggagcgc ctgatttcat ttctcacgta ggtatttatg
    10141 tcaatgaaac aactatgtat gacagtaatt caagcggaat tggttatcac aattggcaag
    10201 attcatattg gcaatcacat agtccagaga ttcgtagagt caaaaaatag gaggaagaaa
    10261 atagatgatt aagagaaaaa atgataataa atggaaatat atttgtggtg ttttaattat
    10321 tattttagtt ttaggtggat tgttatctca acttcataca agtaacattc aagcaaaaaa
    10381 taaaaaattg gatcaagaag ttacacaact agaagaacaa aatagcaagt taagtaaaaa
    10441 tattagtgat ctaaatgcaa atgtagaagc gaacttaact aatgatgatg gcagtgaatt
    10501 aaataaacaa attgctgaag ttgctaagaa atttgttgag ctatatccgg tatatgatat
    10561 tgaaaaagta gaagaaaaga aagctgattt agagaaaata gctactcctt ctgtagctga
    10621 ttctatcgtg ccggacgaca tgattacaac gagtaaaaag actttagaaa gtaaatctac
    10681 tactcaaaaa ggagaagcat attcaagtga tccaacattc aaaagtaaat ataaagattc
    10741 aaaattatat acgaattatg ttaacgccaa tactattaat tattttgctg aagtaaatta
    10801 taaaacagaa tcttctagtg gagatacaga aaatacagtc tatatgcttt ttgaagtaac
    10861 caataaagac ggccaagtgt tggtagataa ttatgaaatc aagtacttaa aatagtgagg
    10921 tgaagtatat gaataagaaa tattccattc ataataagaa ggaaccaaat aaaaaaaata
    10981 aggcacaaat tgacaaagta tataaaaaaa cgaaaggagt aaaattatat ttaggtgcta
    11041 tacttggatt tttagcaata ggaatgataa tcgttttttc tccttatgtg atggggaaaa
    11101 gttatgattt tgaaactgta aaaatgagtt cagcccaaaa tgtaacgtct tctttgaata
    11161 tgtatgttga tgatatggaa ataaataggg aagccggact gtttaaatta gtattgcggt
    11221 atcaagatga tacaggtact aaatcactaa gtaatattaa aagtgactat aaattaaatt
    11281 ttataacgaa caaaaataat gaagatgtta agacaaaagt tgtaaaattg tcagatgaat
    11341 acacggtaat ttattataaa aatctgccta aagatttt
//
LOCUS       DACDCJ010000086        11145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDCJ010000086 DACDCJ010000000
VERSION     DACDCJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.086"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IV038_002367"
     CDS             6..305
                     /locus_tag="IV038_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8342551.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IV038_002368"
     CDS             378..1739
                     /locus_tag="IV038_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8342552.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IV038_002369"
     CDS             1761..2021
                     /locus_tag="IV038_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342553.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IV038_002370"
     CDS             2035..2787
                     /locus_tag="IV038_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP8342554.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IV038_002371"
     CDS             complement(2881..3486)
                     /locus_tag="IV038_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP8342555.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="IV038_002372"
     CDS             complement(3542..4516)
                     /locus_tag="IV038_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP8342556.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5673)
                     /locus_tag="IV038_002373"
     CDS             complement(4894..5673)
                     /locus_tag="IV038_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8342557.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPPDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="IV038_002374"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="IV038_002374"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP8342558.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="IV038_002375"
     CDS             7164..8561
                     /locus_tag="IV038_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8342559.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="IV038_002376"
     CDS             8642..9082
                     /locus_tag="IV038_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP8342560.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="IV038_002377"
     CDS             9229..9555
                     /locus_tag="IV038_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8342561.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="IV038_002378"
     CDS             9556..10305
                     /locus_tag="IV038_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342562.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="IV038_002379"
     CDS             complement(10331..10741)
                     /locus_tag="IV038_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8342563.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3535 a         1948 c         2291 g         3371 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat tttta
//
LOCUS       DACDCJ010000087        11020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDCJ010000087 DACDCJ010000000
VERSION     DACDCJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.087"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            38..126
                     /locus_tag="IV038_002380"
     tRNA            38..126
                     /locus_tag="IV038_002380"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            141..212
                     /locus_tag="IV038_002381"
     tRNA            141..212
                     /locus_tag="IV038_002381"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:174..176,aa:Glu,seq:ttc)
     gene            228..300
                     /locus_tag="IV038_002382"
     tRNA            228..300
                     /locus_tag="IV038_002382"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:261..263,aa:Val,seq:tac)
     gene            310..382
                     /locus_tag="IV038_002383"
     tRNA            310..382
                     /locus_tag="IV038_002383"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Asp,seq:gtc)
     gene            418..493
                     /locus_tag="IV038_002384"
     tRNA            418..493
                     /locus_tag="IV038_002384"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Phe,seq:gaa)
     gene            495..575
                     /locus_tag="IV038_002385"
     tRNA            495..575
                     /locus_tag="IV038_002385"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Tyr,seq:gta)
     gene            582..655
                     /locus_tag="IV038_002386"
     tRNA            582..655
                     /locus_tag="IV038_002386"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Trp,seq:cca)
     gene            658..730
                     /locus_tag="IV038_002387"
     tRNA            658..730
                     /locus_tag="IV038_002387"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:His,seq:gtg)
     gene            742..813
                     /locus_tag="IV038_002388"
     tRNA            742..813
                     /locus_tag="IV038_002388"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Gln,seq:ttg)
     gene            859..929
                     /locus_tag="IV038_002389"
     tRNA            859..929
                     /locus_tag="IV038_002389"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:891..893,aa:Cys,seq:gca)
     gene            950..1034
                     /locus_tag="IV038_002390"
     tRNA            950..1034
                     /locus_tag="IV038_002390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:984..986,aa:Leu,seq:caa)
     gene            1096..1168
                     /locus_tag="IV038_002391"
     tRNA            1096..1168
                     /locus_tag="IV038_002391"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1129..1131,aa:His,seq:gtg)
     gene            1178..1249
                     /locus_tag="IV038_002392"
     tRNA            1178..1249
                     /locus_tag="IV038_002392"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1210..1212,aa:Gln,seq:ttg)
     gene            1267..1351
                     /locus_tag="IV038_002393"
     tRNA            1267..1351
                     /locus_tag="IV038_002393"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Leu,seq:caa)
     gene            1606..2481
                     /locus_tag="IV038_002394"
     CDS             1606..2481
                     /locus_tag="IV038_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8342564.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2585..3349
                     /locus_tag="IV038_002395"
     CDS             2585..3349
                     /locus_tag="IV038_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP8342565.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3366..4778
                     /locus_tag="IV038_002396"
     CDS             3366..4778
                     /locus_tag="IV038_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8342566.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            4795..6119
                     /locus_tag="IV038_002397"
                     /pseudo
     CDS             4795..6119
                     /locus_tag="IV038_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            6132..6590
                     /locus_tag="IV038_002398"
     CDS             6132..6590
                     /locus_tag="IV038_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8342567.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            6882..8183
                     /locus_tag="IV038_002399"
     CDS             6882..8183
                     /locus_tag="IV038_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8342568.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            8210..8470
                     /locus_tag="IV038_002400"
     CDS             8210..8470
                     /locus_tag="IV038_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP8342569.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(9469..10704)
                     /locus_tag="IV038_002401"
     CDS             complement(9469..10704)
                     /locus_tag="IV038_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP8342570.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         3373 a         1775 c         2359 g         3513 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg caaactgtct caagggttcg aatcccttac
      121 tctccgtagc aaatttacct ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
      181 cggtaacacg ggttcgaatc ccgtacgggt cattttaatt gaatgttgga ggtttagctc
      241 agctgggaga gcatctgcct tacaagcaga gggtcagcgg ttcgatcccg ttaacctcca
      301 taatgaactg gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg
      361 ggttcgattc ccgtcggaac cgtcgttctt gttattcaat taataatttt aaaatatggc
      421 gcggtagctc agttggtaga gcaacggatt gaagctccgt gtgtcggcag ttcgattctg
      481 tcccgcgcca ccatggagga gtagcgaagt ggctaaacgc gacggactgt aaatccgttc
      541 cttagggttc agtggttcga atccactctc ctccatttta taggggcata gtttaaaggt
      601 agaacagcgg tctccaaaac cgttagtgtg ggttcaattc ctgctgcccc tgccatggcg
      661 aatgtggcga agtggttaac gcaccggatt gtggctccgg cattcgtggg ttcgattccc
      721 atcattcgcc ctttttattc ttggggtata gccaagcggt aaggcaaggg actttgactc
      781 cctcatgcgt tggttcgaat ccagctaccc cagtttctat aatttattct ttattttgta
      841 gattgataat agtataatgg cggtatagcc aagtggtaag gcagaggtct gcaaaacctt
      901 catcaccggt tcaaatccgg ttaccgcctt agtactaata aatcaacttg ccggcgtggc
      961 ggaattggca gacgcgctgg actcaaaatc cagtgcccgt tgagggcgtg ccggttcgac
     1021 cccggccgcc ggtattgact aagatgtctt catcttagtt ttttattgag aaattaaata
     1081 acatctttta tgatggcgaa tgtggcgaag tggttaacgc accggattgt ggctccggca
     1141 ttcgtgggtt cgattcccat cattcgccct ttattcttgg ggtatagcca agcggtaagg
     1201 caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt tattattttt
     1261 aaatttgccg gcgtggcgga attggcagac gcgctggact caaaatccag tgtccgttga
     1321 ggacgtgccg gttcgacccc ggccgccggt ataattatca atatagcaac tttcttatag
     1381 agagttgcta tatttttttt gtttttatgt aattacctcc aaatcaattc gatacaagta
     1441 ttatcataaa acgagtaaaa aagatattga taacgctttt attatgtgtt aaactatttt
     1501 tgtaaattat gtagggtgct actattcaat taggcacgac ctaagaaagg gttctttctt
     1561 aggtcgtttt ttgtctttaa aaattgtttg gagacaaaaa tatgtatgaa ggagtctgca
     1621 agttgttttg ttttatcata tagtggaggt gaattaacgg tgtatgtgat gaaaaagctg
     1681 aatcataatg ctgtattgat cgatgacgat ggaatagaaa aaattgcgat ggggaaaggg
     1741 attggctttt ctgctaaagt gagtaagcca tttgatgttg aggatgctga caaattattt
     1801 gtattagatt ctaaagaaaa gatcaaacga ttcagtgaga tagtctcgca gattccaatc
     1861 gaatttatgg attttgcgga agaagtgatt gaactgataa cagctaggat ctcctctcca
     1921 ttagattcaa atatctatat tacattgaca gatcatattt attttgctat tcaacgaaaa
     1981 agagaagata gagatgtcac agcaattatg ttgccagaaa tgagattcct ttatccaaat
     2041 gaatatgata tagcaataga agtggtcgag cgaatcaatc atcgatatca aaccgagtta
     2101 gggtcaaatg aagtaggatt catcactatg catatcatta atgctgagct aggagagcct
     2161 aatagtttaa agagcttaaa aatagtagaa atgacgacca cgattttgtc ccatctagaa
     2221 aaatcctatt caaacgttta tgataaagaa tcactgactt atcatcgctt aatggttcat
     2281 atcaaattcc tggttaaacg cttgatttat caggaagaaa cgccagtaga aaatattggt
     2341 ttttttactg aaacatttaa acatagcaaa gcgtattgta cagcactaaa gattaaagat
     2401 atcatcgaac agcaatttca acttgccatt caagaaaatg aaatcatcta tttggcaatt
     2461 catctagcac gaatctatta aaataagggc gtaactatct ataggcgcaa ccaaatgaca
     2521 catagtaaat ggactatgat tatttggttg cgcctatttt agtagaaaaa agaaggagaa
     2581 tgaaatggga aaagtgattt ttaattcgga tgattttggc tatagccatg gtgtgaatta
     2641 tgggattatg gatgcttatc agcgaggaat tttgacatca actacattga tggcaaatat
     2701 gccgggtttc gagcacgctg tgaaactaag aaaggaaatg cctaggctag gtgtaggagt
     2761 ccatttaaca ttgacgtgtg gcaaaccact tctcaaaact gtcgatactc ttatcgaaag
     2821 cggagagttc agatccttat cttattatca gcatccattt aacattgaca aagatcaact
     2881 gtatcaggaa tggaatgctc agattcaaaa aatttatcat gcggggatca taccaactca
     2941 tttagacagt catcatcata ctcatacttt ggatgaaaac caggaggtag ttgttacatt
     3001 agcaaaaaaa tatgatttgc cggttcggag caactttgaa cgaaaagatg aagttcgtca
     3061 tgtcgattat tttgagcctt gtttcgatgt cgtaggagaa actgatgaaa aaaaacgaca
     3121 aatcaatacg agtgtggaag aatatttggc aagtttactc atgatattga gagaagataa
     3181 aacgatcgaa gtcatgtgtc acgcagcgta tttggatcag gaattgttaa atggttcaag
     3241 ttttgtgtat cctcgtactc accaagtcga atttttaatt cattcagcat ttgctcaaaa
     3301 agtgaaaaat gatcctttga ttgagttagt aacatatgag gatatttaat agaggaggaa
     3361 taaaaatgaa tgttaaatca aatgtgcaga agcttggaag agcgatgcta cttccagttg
     3421 cagcgatgcc attagcggga ttgatcatgc gtttatcagc tgatgatatg ctgaacatcc
     3481 cagtaattgg tgcagctgga aatgccgttt ttggaaactt ggatatctta tttgctatag
     3541 gagttgcaat cggttttgtc aaaaataagg acaaaggaat acctgcactt actggttttt
     3601 tagcgattgc taccttgaaa aaaggcttag aaatcatgga cccagaagtg aatatgggga
     3661 tttttggtgg aattatttcc ggtttagtag cggcatggac gtataatcgc tttaaagaac
     3721 aaaaattacc agtagttttt tcttattttg cgggcgaaaa attcccattg actatggtga
     3781 tgattctaca aacaatcgca gctatttttt tgggctttat ctggccgctc atccaaattg
     3841 ggattgataa ttttgcaaaa ctgctcgttg atatgggggc attaggtgtg gggatattta
     3901 tgtttttgaa tcgtttgctg atcccctttg gactgcacca tgtgttgaat acgtatattt
     3961 attacgattt aggaagctat acatctccta gcaaggaagt tttcagagga gagatgacac
     4021 gctttatcaa cggagatcct caggctggac tgtttttatc aggttttttt gtagtaatga
     4081 tgtttggtat accagctata tgtctagcta tctataaagc agcttataaa gaaaataaag
     4141 agatggtcaa aggaatcatg ggaagcggtg caggaacttc atttattgca aatatcactg
     4201 agcctgttga atttagtttc atgtttcttt ctcctatgtt gtacgtgatc catgcacttt
     4261 ttgccggttt agcagggatt gtctgttact tattcaatat acgaattggt tttacattcg
     4321 gagcatgtat tgttgattat ctcatcaatt ttcgaatcgc taccaatgct attttgattc
     4381 ttccaatcgg aattttcttt tttgccttgt attacgttac tttttattat ttgatcaata
     4441 agagaaacat tcagacatta ggacgtgaag ctaaggcaga gtttggcaat gaagtaacct
     4501 tagaagaaac agagttaggg ttagcaagta aaaattatga atatatggca acaaaaatgc
     4561 tacaagcgtt tggaggaaaa gccaatatcc tggatgtcta tagttgtaat acacgcctac
     4621 gggtagaggt agtggatccc accatggtcg aggaacagcg aatcaagcaa ttagggataa
     4681 gcgggatcat taaaccaact gaaaaaaatt atcaaattat catcggctta gaagtgactt
     4741 atgttatggc agaattcaat aaattattag aagaatagaa aggaaaagat gatgatgaaa
     4801 aaacagatta tcactatagc tggaggcggg agtacgtata caccaggaat cgttcaagcc
     4861 attttgaaca atgaaaaacg gttgccatta tctgaaatac gtttatatga tattgatgaa
     4921 gaaagaaata tggatatgta cttgattgtt aaatttatgc tgaaaaaaaa ggtttttcaa
     4981 atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc gattttgttt
     5041 tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag attccactta
     5101 aacatggatt agtaggacag gaaacgtgtg gactcggagg atttgcttat gggatgcgct
     5161 caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc aaagcatgga
     5221 ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga
     5281 atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg attgcagtga
     5341 attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac cactttggct
     5401 ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag attattgaaa
     5461 aactaataac aaaagaaatg caagtcgccg attttaatat aggagacaaa acttggcaaa
     5521 aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc ccgaataatt
     5581 acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa gagtatacac
     5641 gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct gataaaatac
     5701 gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga caatatatcg
     5761 tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta atcgttccta
     5821 atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg gcatatgttg
     5881 gtgcaactgg tgtggagaca attacactaa gaaaaccaat cagtgatttt cataaaggat
     5941 tgatggaggc acaagttgca gcagagaagt tactagtaga tgcttacttt gaaggttctt
     6001 atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc agagtcgcaa
     6061 aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca ttaaagtaga
     6121 ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta gctagtggtc
     6181 aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg ataggagttg
     6241 gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga ataatcgata
     6301 atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga aaaatactga
     6361 tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata cgggttgaaa
     6421 gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag aagttggagc
     6481 aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa ggaaatttat
     6541 taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag tgtacgatga
     6601 aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg aaatgataag
     6661 tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt tttgttgctc
     6721 ctatgattta gaaaacttat tttcaggatt cttaaaaata aatttgatta tttaagagaa
     6781 tatattgaaa taaaatttct taattatata attagttatg aacattcttt gcgttgtcat
     6841 gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag ctgatgaaat
     6901 gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt catttattag
     6961 caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca ttttttatgg
     7021 tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat tcggcaacga
     7081 ttgttttacc ttttaggatg acgatgggat tgatggctat ttatgcaact tttgttatgg
     7141 gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata ttatctttag
     7201 gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaaaag gcacctcttg
     7261 gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg atcttatcta
     7321 tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact atcaaaatgc
     7381 cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca ggtgcagtca
     7441 ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac gctaccttat
     7501 tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt gttttactac
     7561 caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt attgtagggg
     7621 ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg
     7681 gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa tggacggtaa
     7741 caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc ttctgtaaat
     7801 caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc aatatcaatg
     7861 agcccatgat ttttggagct cctatgatgt tgaatcctta catgttcatc ccgttcaact
     7921 tagttccttt agttttgact attatcagtt attttgcagt caaattagaa atggtcaata
     7981 gatttactgt tttgccaact tggacattac cagctccaat cggcggttat ctttcagcag
     8041 gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca tttattatct
     8101 attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa agtcgtggag
     8161 aaaacgaagt ccaaactgtc taagtttggt ttttagaagg tgggaaacaa tgtattggct
     8221 gattttattt tttgttttta tctttttatt aacggctagt caccttatat tgaatatgct
     8281 agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat ttttgatcgt
     8341 gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg ggacatatgt
     8401 actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt ttgtcacatc
     8461 gaagctttaa atgttctaaa agtggttgtg acagaagtgg ttagcttcaa gaaataagaa
     8521 gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt
     8581 tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata tgggactgtg
     8641 acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta actgttttgg
     8701 cagaaagaag gaggcaaaaa tgaaaatttt atcagcaatg gattaagaat tagtcgatct
     8761 actaccaaag tgatcaaggc aaatatagat ttgggagctt acaaccatat agtaggggct
     8821 gcagtatggt gtatgaatgg taatatgtat gatgagagtg tttattcaaa tcatgaaacg
     8881 tgtatagaaa taattgcgct gggaatagca atcagtcatg gtgaacgtga cttttagttt
     8941 atcgttgctg acgacataga acattctgac atgatctact cagcatgtgg ggattgctgg
     9001 cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga agagggaggt
     9061 tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt gaagggacta
     9121 gcagcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt taagaaaatg
     9181 tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc caaccagaga
     9241 aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct catttggatt
     9301 gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta atttaagtaa
     9361 ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg gtctcgaata
     9421 gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact atattgggtg
     9481 gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta ttggaaaaac
     9541 ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat
     9601 ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt attttacaca
     9661 atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg gtttgccgga
     9721 ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat caatgaattc
     9781 atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt cagattgaat
     9841 gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt tttgtaagaa
     9901 gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga aatagataat
     9961 acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag gcaaggaagt
    10021 actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct gttcttcttc
    10081 caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt gtaaacttgt
    10141 ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg
    10201 ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa
    10261 acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata
    10321 gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt
    10381 cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt cactagtgat
    10441 acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt
    10501 tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat actcaaagcc
    10561 actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac agtaataacg
    10621 gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc
    10681 aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct atcccttcgt
    10741 ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt ttgaatatta
    10801 aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcaa gttgaaactc
    10861 caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg ccagataaat
    10921 acgagaagtt gtgtcattaa aaataaacat gagaacataa aaaataaaat aattatatta
    10981 attgataggt gtgctgtcgt cattatacac acgtaagaaa
//
LOCUS       DACDCJ010000088        10749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDCJ010000088 DACDCJ010000000
VERSION     DACDCJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.088"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(23..1807)
                     /gene="pyk"
                     /locus_tag="IV038_002402"
     CDS             complement(23..1807)
                     /gene="pyk"
                     /locus_tag="IV038_002402"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP8342571.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(1881..2843)
                     /gene="pfkA"
                     /locus_tag="IV038_002403"
     CDS             complement(1881..2843)
                     /gene="pfkA"
                     /locus_tag="IV038_002403"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP8342572.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(3011..6325)
                     /gene="dnaE"
                     /locus_tag="IV038_002404"
     CDS             complement(3011..6325)
                     /gene="dnaE"
                     /locus_tag="IV038_002404"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP8342573.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            6556..6741
                     /locus_tag="IV038_002405"
     CDS             6556..6741
                     /locus_tag="IV038_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP8342574.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(6803..7999)
                     /locus_tag="IV038_002406"
     CDS             complement(6803..7999)
                     /locus_tag="IV038_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP8342575.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(8169..9605)
                     /locus_tag="IV038_002407"
     CDS             complement(8169..9605)
                     /locus_tag="IV038_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP8342576.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            9898..10707
                     /locus_tag="IV038_002408"
     CDS             9898..10707
                     /locus_tag="IV038_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8342577.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3208 a         2249 c         1918 g         3374 t
ORIGIN      
        1 ttttattttt tattaagcta aattagatag ctgttgtttc gccacggtaa acgatgccgc
       61 gacgtgaatc aacggtaatc aattcacctt ctgtaattgt tgttgttgcg cctttagcac
      121 caacgattac aggaatatct tgtgcaattc ctacaactgc agcgtgagaa gtcaatccgc
      181 cttcttcaac gataacagca gctgcttttt cgattgcagg catatattct ttgtcagttg
      241 atggtacaac tagaaccatt ccgtcttttg ctttttttac agcttcttca gcagatgaag
      301 caataactgc atttgcaacg acagattttt gtccaacgcc ttgagcttct aataatttag
      361 aaccaatcat ttgaattttc atcacgttag ttgttccgcg ttcaccaact ggtacaccag
      421 cagtgatcaa gattaaatcg ccttctgatg cgaaacctaa ttcaacagct tttttagctg
      481 ctagttcaaa catttcatca gttgttgatg gtttttcagc aactgttggg taaacgcccc
      541 agtttaacat caaaccacgt tttgtgcgtt cgtcaaaagt aacagccaag atatctgcat
      601 ctggacgata ttttgaaatc atttttgcag tgtatcctga ttcagttgca gcaacgattg
      661 ttttcacgcc tagatttttt gcagcacgag ctacagataa accgattgtt tcagttacat
      721 ctgttttatc gaattcgttc aattggaatg tacctaattc tgataatgca ttttcagctt
      781 cttggtcgat acgtgccatt gttgaaacag cttcaactgg gtagtccccg tttgcagatt
      841 caccagatag cattgttgca tctgtgccat caaatacagc gttagcaacg tcagaagctt
      901 ccgcacgtgt tggacgtggg ttttcttgca ttgattctaa catttgtgta gcagtgataa
      961 ctgggatacc agcagcatta cattttttga taatgcgttt ttgaaccatt ggtactagtt
     1021 cagctgggat ttcaacaccc atatctccgc gggcaatcat aacaccgtca gaaactttga
     1081 tgatttcgtc aatgttgtcg ataccttctt gagattcgat tttagggaag atttgaacat
     1141 gagtcatgtc tttttcttct aagatttcgc ggatatctag aacgtcttgt gctttacgta
     1201 caaaacttgc agcgataaag tcgatatcgt tgtctaaacc aaaacgaata tcgtcagcat
     1261 cttttggagt aatacctggc aaactaatag aaacgcctgg tgcatttacc cctttacgtg
     1321 atcctagcat acctgcattt ttaacttcta cgactaattc acggtttgct tcgtcttttt
     1381 caatgatttg catatcgatc aaaccatcat caaataaaat gtgtccgcca acgtgaacat
     1441 cgtcaaataa tcctgggtaa gtaaccgcga ttttttcttt tgaacctaca tgttcaggat
     1501 ccatagagat gcgtgtttga tctcctactt caaatttgat gtatcctgca cgaccaaaat
     1561 cagcttctgt tgtgccttga acagttgtac ggatttcagc tccttttgta tctaaaagaa
     1621 taccgacatc ttttcctgtc tttttaactg cttcacggac catagtcata cgtgataatt
     1681 gttcttcatg gtcaccatga gaaaaattaa agcggaagac atttgcccca gattcaatca
     1741 attgagaaat aatttctact gagttacttg ctggtcctaa tgtactaacg attttggttt
     1801 ttttcattta aaaaactctc ccattcaatt ttgtttattt aaagctgtac acagcttaaa
     1861 tagcttggtt tctttcactc ttaaaatgaa agtgaattat ttagatcata tagtgataag
     1921 tctggtttat gtttgttgtt ttcaagtgta tcaatgatgt cagacgcaac gatttcgttg
     1981 tcatggatac cgacacataa tccacctttt ccttcttgaa gcaattcgac agcgtaagca
     2041 ccgaatttac ttgccaacac gcggtcgcgt gcgcttggtg ctccaccacg gacaacgtgt
     2101 cccaagatag atacacgtgt atgatagtca ccaaattctg aaagtttctc agcgaattca
     2161 tttccgccca ttacaccttc agccaagatg atcaagcaat gtttcttccc gcgatcgcgg
     2221 ccttcttgga tttttttagc aactgaagcc atatcaaaat catgttctgg gatgatgatt
     2281 tcatccgctc cgccagccac acctgaccat aaagcgatgt ctcctgcatt gcgtcccatt
     2341 acttcgataa cgaatgtacg aacatgagaa gtcgctgtgt cacgaatacg gtcaattgat
     2401 tctagtactg tattgattgc tgtatcaaaa ccgattgtga aatcagtccc aggaatatca
     2461 ttatcgattg ttccaggaat acctactgct gggaaaccgc gttttgtcaa agccattgcg
     2521 ccatgataag agccgtctcc tccgataacc actagacctt cgattccgaa ttttttcaat
     2581 tgttcgattc cttttaattg accttcttca gttgcaaatt ctggataacg tgcagaatac
     2641 aagaatgtac caccacgttg gatcttgtca cctacatctg ccacatcaag acgacgaata
     2701 tctccagcaa ctaaaccggc aaaaccataa ttgatcccgt atacttccag tccttcgtaa
     2761 atacctttac gaacaacggc acgaacagct gcattcattc caggtgcgtc tccgccactg
     2821 gtcaaaattc cgatgcgttt cataataaac attcacctca ttaaaatact ttatcctaag
     2881 tatgacttag gtttacaacc atttttgcgt taaccacgac taacattcta acattatttt
     2941 tctcacttgt cttgatttac ggataaaaaa atgaaaaaaa atgaaagcaa gtagtatttt
     3001 ttaccgtaag ttatttgaaa accacattat cttcgccaag cagataaata agttgccgct
     3061 ttatactctt gctgtcatcc acccaataag catctgacag aactactttt cgttgcgttt
     3121 tttcgtgata cataactact ggtatatatc ctgggtgttt ctgaaaaatc acttgtaatt
     3181 gtttcattat ctcgggatct tcccgttctt ctgtaatacg caaatagcaa gtttgtgaag
     3241 gatactgggc ttctaactgg ttaggatcag caaagctttc tccaattagt tgcagttcct
     3301 gattatattt gctgatttcg ctccgccctt gaacaaagat cactcgatca acggcaaaca
     3361 aattacgata tttacgataa agagttggaa aaactgtgac agaaatttct ccactactgt
     3421 cggttccttc aacaaaggcc atcagttctc ctttttttgt ccgaatttct ctaacttcct
     3481 tgatcatcac taataaattg actggttgat tagggacgat atctgtaatc agctgaactt
     3541 gtttagctaa acgaagacga tcaaaccctt ctgcaggatg acctgacaaa taaataccta
     3601 agtactcctc ttccagcttc aagcgttctt ctagtgtata gtcagcgact tcttcttctt
     3661 ttaatgccat gattcccagc aagtcaagac ttccaccact gtaaacgata ttttggattt
     3721 ttccttcgag atcaagcatc aattgtttcc tgttaggaga taattcatca aaaactccta
     3781 ctgctgtcag tgattgcagt aactctaatt tcagccaacg cttatcgatc cgtatgagga
     3841 attgatccac tgactggaac gggccatttt gttttctttc gtttaatata tcttctacaa
     3901 aatcacgccg aatacctttt atcgctgtca gtccgaatct gatttcttcc ttattgacca
     3961 actcaaaact ggaattactt tgattaatgg acggcgagga aagtttgagt ttatttttac
     4021 gtgcttctgc aatatattct ttcaattttg atgtgttatg ccgaacagaa ttcatcaggc
     4081 tggtaaagaa agcacctgga tgatgggctt ttagatacgc catttggaat cccacgaatg
     4141 aataagcaaa tgcatgtgac cgattgaatc catagtttgc aaatttttcg atatattcat
     4201 atacggtcgc tgctgttttt tccgaatatc cctgttgtaa tgctcctgat acaaaatgcg
     4261 acttttcttc atctaatacg tcttttttct ttttactcac tgcccgtcgc agaatatctg
     4321 actgccctaa tgtaaatcca gccatagtcg aggcgatctg catgatttgt tcctgataga
     4381 cgatcactcc atatgtgttt ttcaaaatcg ggatcagtga atcatctggg tagcgaatgg
     4441 cttcttttcc tttcttcctg gcaatgaagg tatctatatt ttgcataggg cctggtcgat
     4501 acaatgcatt gactgcggca atatcttcga tgttttctgg atctaatctt cgtaaaacgt
     4561 tccggatacc agcagattca aattgaaata ctcctgaggt ttcgcctcgt tgaaacaaga
     4621 gcagcgtttt tgcatcattt agcgggatct cttttaatcg gatctcatac ccttctactc
     4681 gcttaacagc tttcaaagta aaatcaataa tcgataagtt gcgtagtcct aaaaagtcca
     4741 ttttcaaaag accgattgct tctacctcat tcatcgtata ctgggtcaga aaagcttcat
     4801 cagaaccgcg ttggagcgga actagttcaa gtagattctt atcgctgata actacacctg
     4861 ctgcgtgagt cgaaacatgt cttggcaatc cttctaaaat cactgcagtt ttgaataaaa
     4921 gttggttttt ttcgcttgcc tgaactaatt ctactaattg atgcgaagtt ttataagctt
     4981 gggtcaatgt gattttcaat tgattgggta ttgcttttga ccagcggttt gcttcacttt
     5041 gcgataaacc aaacactcta gctgcatctc taagaaccat ttttgctgcc attgtgccaa
     5101 atgttgcgat ttgtgccata tgatattgtc catacttttc tcgaacataa ttcaatactt
     5161 cttcacgtcg attgtcagga atatccaaat cgatatctgg catcgtaaag cgttcggggt
     5221 ttaaaaagcg ttcgaacagc aaatcatatt ctaatggatc cacatctgtg atttctaaca
     5281 cataggctac taatgaaccc gcagcagaac cacgacctgc accggtaacg attttgtttc
     5341 tatgagcaaa agccattaca tcccatacga tcaagaaata atcatcgaat cccatttgat
     5401 ggatgacaga taactcgtag tccaaacgtt cttggtattc ctttgtatag tcagtgattc
     5461 ggcgttccac ttgcgaatag cataactctc ttaaatattc tccagcttgt tttccattag
     5521 gaactggata atgcggaagt aatttctgat ggaactggaa ttcaaatgaa atcgattcag
     5581 ctaaacgctg ggcgcctatt acagcttggg aaagacccgc tttttcgaat cgtttgatca
     5641 tttcttgttc tgaaagaaga tagacatttg ctgttagtgg taaagatgca gataaatttt
     5701 ctaaaggtat ttggcttccc tctttgatat gtttcatcac ttcaagtgca aaaagttctt
     5761 cttgttgtaa actagcgatg ggatgtaatg cacaaagcga ataattttct tttatcacat
     5821 actgatacca ttcaggcaat tgatcaggcg tgttttctgt caagccgata taaaaatttc
     5881 tagtaaaaac attttcaagt aaaaactgcc aatgaccgaa aaagttatcc tctttattga
     5941 aagcctcaga aacagaatga tttgccggaa caacaaacgc tagatggtcg gtttctgtca
     6001 tttctgcaag ttccaatttt tcttggatca tttttcggct ggaaagtttc atcagctgct
     6061 gataacctat ttcatctttt gcgtacgcaa gcaagccata atttttttct tcataagtat
     6121 aactaagatg taatccaatg attggctgta ctccttcttc tacacaaata cgatagaatt
     6181 caggtgcacc gtataagaca tctttatccg ttatggctaa tgaagtgtag ccttgttttt
     6241 ttgcttcttg tacatattct cgaatacgta tcgtactttg taaaagagaa taagaagtaa
     6301 ttgtataaag ttgtgggaaa aacatcttgc attccgcctt ttttttcgtt aaaattcact
     6361 agcgttaaaa ggtttgatcc ttttactttc attaagatag caggcgctga agttgattat
     6421 tccacttcat ttgcttgctt gtaatctgta ggaatgagtt ctttcgcaag aactcaaatt
     6481 ttattgagat ttggtctcgt ttctttacat tcaaagaaaa tctggtaaaa ttaagataga
     6541 aaagaggtgt tctttatgcg atatatcgtt acattattct gggctgtcgt tctaggtcaa
     6601 gtagtcggtt atattggtgc tgcattaaca agcggaacat atgattttac tttgacaaca
     6661 atcatttcat tcattgcggg tgtcattatc cttttgattg gtgcagttgc gccaagaaaa
     6721 gaaacctctg cccattcgta aaaaaacagc caagagcttg agatttctca agctcttttt
     6781 tattttctac tcctcaccac cattataatc tgctgcgaag aactttacga aagttgaata
     6841 aagaaagaag aatatttgac agaacaaaca aacttgtcac aataaaaaca gcactgtaat
     6901 tataagcatg cgcgactgtc gatcctagca tgggacctaa aacttgtcca aagttgctaa
     6961 acatctgatt gtagctatag attcggctga ctccttcttg aggcgtaatc ttactgatca
     7021 gtgtattgac agaaggcatc aaagcgccag ttgaaaagcc taaaataaag cgaagtatac
     7081 ctaattggag cggtgtagtg acgaatgcca taggtaaata acagcaaaat gataaaatga
     7141 gtcctccaat tagaactttt tgattcccaa tcttatcccc tagttttcct agtttcggtg
     7201 aagaaaagac agcggaaaca ccagctatcg aaacaatcaa gccgctgaca aaaaggatat
     7261 tttctgtact tccgctcaat tcacgaatat ataaggtcaa aatagggctg atcgtagtga
     7321 ttcctatctg taaaatcaag gtcgtaatga agagaccgat caaaatagag agatgatcca
     7381 tttttgaaaa aatttctttg gtgcttatca gatctttttt ctcgattgga tggaaatctt
     7441 ctttgactaa gaaaatggtc aataccgttg tgatcatcaa caaagcccct gtgatcaaga
     7501 atacattttc cataccaaac cactgagcca gtgcaccgcc cattgaaggt ccgatcagat
     7561 ttccagcaat cgctccggta gacaatgttc ctaacgccca tccgctcttt tctctaggtg
     7621 cctgtgaagc aatcatcgct gtggcatttg gaatatatcc agacaaaatc ccattccaaa
     7681 accgcatgat caaaagccaa tacgcgtttg gtacaaaagc caaagatccc attgttaatg
     7741 tcatccctgc tgctgcacgg atcatcatga gttttcggcc ttttcgatca gctagatttc
     7801 cccagattgg tgcaaccacg gcagatgcaa aagcagtcac agagattgct agtcccgaaa
     7861 acaattcgat ttgatctttt ggtgttccta gttgctcaac ataaactggt atgaatggca
     7921 taactaaact aatacttgca ccagtgaaaa aacagccaat ccacgcaatc actaaatttt
     7981 ttctccaatt aatctccatc tatcatccct ctttcgtctc ttttatcata cctattttcg
     8041 aataaacaag caacgatctc gccagtaaaa agagaaaaat cccgcaaagg tcattttata
     8101 tgtaacgttc tttttatgta aaacaaaaag agatcagaac aaatactgtt ccaatccctt
     8161 ctctttagtt aaattgttgc tagaatgatg aagttgataa tgaataaaat atcaacgatc
     8221 cacaaagcaa cagaaacttg attgtatttt cctttgatta ctttcacaat tgtatagaaa
     8281 ataaatcctg ctgcaatccc ataggaaatg ctatagcaca atcccataaa gatcgatgca
     8341 aagaaggccg gtaaagcttc ttccatatct agccatttga tgtctgcaaa agaagccatc
     8401 atcattactc caactaaaat caatgctggc gcagttgctt gatttggtac aatggcgatc
     8461 agtggagaga ataaactact taatgcgaat agcactgcca ctactactga agtcaaacca
     8521 gtacgtccac cggccccgat cccagctgca gattcgacat aagttgttgt attcgatgta
     8581 ccaaagatag cccccacaga agtagcgatc gcatcggcaa atagtgcttt atccattttt
     8641 gttttgaacc cacgaccatc ttccaacgcg atctcgtctt cttttgagaa aatacctgtg
     8701 cggcgtcctg taccgataaa tgttccgatc gtatcgaaag tatcagataa actgaatgcg
     8761 atgatcgtca tcaatacttg aggaatcttg gaagaatcac tgaataaaga ttgcatgcct
     8821 tcagaaccaa atgctgcacc gaaagttgtt cctaattctt taaatgaatt acctaacgaa
     8881 ttcgtttgcc aatcaatgga acttaaatca acaacaccca taaaaatacc aaggactgtg
     8941 gtcgctacga taccgataag aatagcccca cggacatttt ttacaactaa aatggctgtc
     9001 agtaccaaac caatcacagc caataagata ggagcgttgt caaagttggc taacgcaggg
     9061 acgattccac catttatcgt aacatttgtc gcttttccgc cttcaacgac agagctcgaa
     9121 atagctgact ggtctgctga aaaagataag aatcctgcat ttttgattcc gacatacgca
     9181 acgaaaatac cgatcccgcc accaatcgca tgttgaagac tttcgggaat cgcccgaata
     9241 atcatttttc gaatatttgt gacagtaata aaaatattga ttaatccgca aataaacacc
     9301 attgccaagg cttgttgcca agaataacca agcccaaaaa cgaccgtaaa agtgaaaaat
     9361 gcgttcaatc ccataccagg tgcctgagca tatggtacat ttgcgaacaa tcccattacc
     9421 agcgtaccaa taatcgatgc aatgattgtc gctagaaata ctgcttggaa gggcatacct
     9481 gatgctgaca aaatcgtcgg atttacgaat aagatataac tcatggcaaa gaatgtcgtt
     9541 actcccgcca ttatttcagt tgatacattt gtattgtttt tctttaaacc gaaaaacttt
     9601 tccataaaaa gttccactcc ctgaattttt ttctgatgga aaactataat aataaaaaat
     9661 aagtaaaata gggcaaatcc atcgaacaat ttcaaattat atagacgttt cattcgcttt
     9721 tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt tagcttatgt
     9781 ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt gctcttgtta
     9841 attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg acccttattg
     9901 aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga ttcgatgatt
     9961 acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca tgtcagtatt
    10021 gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga attaacaaca
    10081 ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg ggatggtgaa
    10141 agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa aaagcagcta
    10201 aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag ttttgatttc
    10261 tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct ttctcctaaa
    10321 aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt tgcaggagct
    10381 gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac atggggtgga
    10441 cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg cgtgcaagag
    10501 attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga cgagcataat
    10561 gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg tactgaccaa
    10621 ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg tttagctgtt
    10681 tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa agaggttgtg
    10741 acaattttt
//
LOCUS       DACDCJ010000089        10613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDCJ010000089 DACDCJ010000000
VERSION     DACDCJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.089"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            239..2470
                     /gene="pflB"
                     /locus_tag="IV038_002409"
     CDS             239..2470
                     /gene="pflB"
                     /locus_tag="IV038_002409"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP8342578.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2714..3475
                     /gene="pflA"
                     /locus_tag="IV038_002410"
     CDS             2714..3475
                     /gene="pflA"
                     /locus_tag="IV038_002410"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP8342579.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3560..4486
                     /locus_tag="IV038_002411"
     CDS             3560..4486
                     /locus_tag="IV038_002411"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP8342580.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4668..5561
                     /locus_tag="IV038_002412"
     CDS             4668..5561
                     /locus_tag="IV038_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP8342581.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5695..5820
                     /locus_tag="IV038_002413"
     CDS             5695..5820
                     /locus_tag="IV038_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8342582.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5954..6586)
                     /locus_tag="IV038_002414"
     CDS             complement(5954..6586)
                     /locus_tag="IV038_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342583.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(6734..7276)
                     /locus_tag="IV038_002415"
     CDS             complement(6734..7276)
                     /locus_tag="IV038_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP8342584.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            7411..7800
                     /locus_tag="IV038_002416"
     CDS             7411..7800
                     /locus_tag="IV038_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342585.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            7804..8568
                     /locus_tag="IV038_002417"
     CDS             7804..8568
                     /locus_tag="IV038_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP8342586.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            8697..9644
                     /locus_tag="IV038_002418"
     CDS             8697..9644
                     /locus_tag="IV038_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8342587.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            9774..10514
                     /locus_tag="IV038_002419"
     CDS             9774..10514
                     /locus_tag="IV038_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342588.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         3729 a         1782 c         2111 g         2991 t
ORIGIN      
        1 aatacaggtg gcgtctcttt ttcattcatt tttaataaat ccttatacgt ttgcaaaaaa
       61 agatgaaagt gttcacaatg cttaaatgtt ggtatgtcaa cgttgattta aatatatgta
      121 aataaaacat aaaaattgtt aaataaaaaa caagaaaacc cttgcaaaga tagggtgctg
      181 tgttatacta atcacgtaaa aactgttaca taaaacagaa atttaggagg tccatacaat
      241 ggagcaatgg aatggattta aaggtaccaa atggaaagaa ggcgtagaca ctagagactt
      301 tattcaaaat aactacactg aatatcgcgg tgatgacagc tttttggaag gcccaacaga
      361 agcaacagaa aaattatggg atgaattgca aaagttaaat gatatccaat tcgagcgtaa
      421 cggtgtctat gatatggaca cagaaatcgt atcaactatt acttcacacg atgcaggcta
      481 tttggataaa gatttggaaa aaatcgtcgg tctacagaca gacaaacctt tgaaacaagc
      541 attcatgccg ttcggcggaa tcaatatggc taacaatgcg ctaaaaagca atggctatac
      601 accaaacgat gaattgacac atattttcac tgactggcgc aaaacacata atcaaggtgt
      661 atttgacgct tatactgctg aaatgcgtgc tgctcgtaaa aacaaaatca ttacagggct
      721 tccagacgct tacggtcgcg gacgtatcat cggtgactat cgtcgtgtgg cattgtatgg
      781 aatcgactat ttgatggaac aaaagaaaaa agatcatgat atgactggtt atcatacaat
      841 gagtgaagaa gtgattcgtt tacgtgaaga aatcactgaa caatatcgtg cattagcgca
      901 aatgaaagaa atggctgcga aatatggcta tgatatttct aaaccagctg caaatgcaaa
      961 agaagctgtc caatggctat acttcggata tttagcagca atcaaatcac aaaatggtgc
     1021 tgcgatgtca atcggacgta tctcagcatt ccttgatatc tatatccaac gtgatttgga
     1081 taatggagta atcacagaaa aagaagcaca agaattgatc gaccacttag taatgaaatt
     1141 acgtatggtc aaattcgcgc gtacacctga atataaccaa ttattctcag gattccctat
     1201 ctgggctact ttatccattg caggtatggg attagatggt cgttcattag ttacaaagaa
     1261 tgatttccgt ctgcttcata cattaacaaa tatgggacct tcaccagaac caaacttgac
     1321 tgttttgtat tctgaacacc ttccagaagg attcagaaca tatgctagca gaatcgctat
     1381 cgaaagttct tcgattcaat tcgaaaatga taatcagctt cgtgaacaat ggggttctga
     1441 tgattgtgcg attgcttgct gtgtatcagg aacagttgtc ggaaaagaca tgcaattctt
     1501 tggcgctcgt gctaacttgg ctaaaacact attatatgcc atcaatggcg gagtcgatga
     1561 agtgactggc gcccaagttg gaccagaata tcgtccaatc acatcagaaa tactagactt
     1621 tgaagaagtg aaacataact tcatgaatat gatggattgg ttagcagaac tatatgtcaa
     1681 tacgttgaac atcattcatt atatgcatga taaatattct tatgaagcag ctcagctggc
     1741 tttgatggat tctgacttgc aacgcacatt tgctacaggt attgctggga tctctcatgc
     1801 ggcagacagc ttgtcagcga tcaaatatgc aaaagtaaaa gcaatccgta atgaaaaagg
     1861 aatagcagtt gattttgaag tggatggcga cttccctaaa tacggaaatg atgatgaccg
     1921 tgcagatgaa ttagtttcat ggatcttgga atacttcatg ggacaaatca aacgtcataa
     1981 aacttaccgc aactcaaaac caacaacttc attattgacg attacttcaa atgttgttta
     2041 cggtaaaaat acaggaaata ctcctgatgg ccgtgaagct ggaaaaccac ttgcgccagg
     2101 agcaaatcca agttacaatg cggaacaaag tgggttatta gctagcctga actcaacagc
     2161 taaaatgaat tacggctgtg cattggatgg aatttcaaat acccaaacaa tcaatccttc
     2221 aggtcttgga aaagaacaag atacaagaat cgaaaacttg cgtaatgtct tagatggata
     2281 cttctcaaaa ggagcttatc atttgaatgt caatgtattc agtaatgatt tattgcgtga
     2341 tgcaatggaa aatccaatga aatatccgaa cttgacgatc cgggtatctg gttatgcagt
     2401 taaattccgt gacttgacaa gagaacaaca attagatgtc ttgatgagaa cttcacacga
     2461 cagaatgtaa aaagttcgtg aaaaaagttt ttcgacttag cgtcgaaagg acgttgaata
     2521 gccatcgcat caaaatcgaa taatcagtat gcttatttga aagaaacgat ttacaggggc
     2581 aggacttgta tgttccgtcc ctgtaaattt tacacataac gaagcggcaa ttgcgaaaaa
     2641 ttttgttcag aaagcttcgc ttcgacagct aacttgtact taaaatgaaa tcaaagaaaa
     2701 gatgtgaact taaatggaag aaaaaacaat cggctatgtt cattctatcg aaactttcgg
     2761 ttcagtagac ggacctggac tgcgctttgt cgtgtttatg caaggatgcc gcatgcgttg
     2821 ccaattttgt cacaatccag atacttggaa tattggcgga gggaaagaat acacagcaga
     2881 tgaactttta gataaagcag aacgcttcag accttactgg ggagataaag gtggtattac
     2941 tgttagtggt ggagaacctc tattgcagat cgattttttg atcgaactat ttaaaaaagc
     3001 aaaagagcgg aagatgcata ctacattgga tacatgcgga aaacctttta cgtatgaaga
     3061 acctttcttt tcacgttttc aagaattgat gaagtataca gatttactgt tatttgacat
     3121 caaacacatc gataatgaag agcataaaaa actaacgcat tgggacaatg aaaatatttt
     3181 agaaatggct caatatttgt caaaaatcaa caaaccggtt tggattcgcc atgtactagt
     3241 tcctgagcga agtgattatg atgaatattt gatccgacta gacaacttta ttcaaacctt
     3301 gtcaaatgta gacagagtag agattcttcc ttatcatacg atgggaaaat acaagtggga
     3361 aacacttggt ttgaaatatc cattagaagg gatcgaacca cctacaaaag aacgtgtcga
     3421 aaatgctaaa cgattgcttc atacagaaga gtatacaaaa tatttaacgc gataggtcgc
     3481 atcggaaaga agataagtat tgcttctttc cgtttttttg ctattattaa ataggagaaa
     3541 tcacaaagga gcgaatgaca tgtcaaaaat tttagttttt ggtcaccaaa atccagatac
     3601 agatgcaatc ggggcagcca ttgcgttttc acacttgcaa aatgaattag gtaaagaaac
     3661 agaagcagtt gctctaggta cacctaatga agaaacacaa tacgcattag accactttca
     3721 gctatctgca ccacgagtag tagagtcagc taaagcagaa aatgcagatg tcatgcttgt
     3781 ggatcataat gaattccaac aaagtatttc agatatcagc gaagtaaata ttttagctgt
     3841 agtagaccac catcgtattg caaactttga aacagctaat ccattgtact atcgtgcaga
     3901 accagttgga tgtacaagta cgattatttt aaaactatat aaagaaaacc aagtaacaat
     3961 tcctaaagaa atcgcaggaa tcatgctctc agctatcatt tcagatacat tgttgttcaa
     4021 atcaccaaca tgtacgcctg aagatgaagc agctgcaaaa gaacttgcag caatcgctga
     4081 agtcgatcta aacacttatg gacttgaaat gttgaaagca ggaacaaatc taagtgataa
     4141 atcagctgcg gttcttcttg atttggatgc aaaaagtttc ccaatgggca gttccaacgt
     4201 tcgtatcgct caagtcaata cagtggacct taatgaagtg atggatcgac aagcagagct
     4261 agaagcagca atggaagatg aaatgaaagc aaatggctac gatctgttcg tattagttgt
     4321 cacaaacatc ttagacagtg attcagaatt acttgttgct ggtaaaggaa aagaaaaagt
     4381 agaagaagct ttccaaacaa ctttgaccaa caaccgtgct ttgcttaaag gtgtcgtttc
     4441 acgtaaaaaa caagtcgtac ctcaattgac ggctgtattt agctaatcaa atggtagtat
     4501 gaagcattca aaatgaatgg ataagcgatt gatataaaaa gaaacataga acatactaaa
     4561 ggccgaagaa atggaggaga ataacagtca attatgactc ttgttcttcc tcctttttac
     4621 gtaaaagcct tttaagtaaa ataaaacgga tagaaaagag aacatatatg aactattatt
     4681 caaaaaataa ggaaaacttt tatcaaaaac tgaccgatga tccgcttttt tcttcattga
     4741 ccgattatct atatgaacat cgagaacaag aaacgattct aagagaattg aaaaaagaat
     4801 tttcgcaaaa taaattttcc cattttcttg atttattgat cgatgcagga ttaatcaaga
     4861 gggaagaacg aagatatcat ttgaactttc caatatttga tcctaaagat tacttgcaac
     4921 aagccacttc ggctgctgaa accatagctg aacaattgaa gagactctct gttgatgaac
     4981 aaaaattagc gatgggagaa gttatttggg cgtattgttt cgaagatgaa cggaaagaag
     5041 cgtattttta tggagtaaga aacagccgag agacagaatt gctgaggaca actgcaggaa
     5101 atgagaagta ccgttttatc acactttcct ctatagaaca cttcccgctt actttggcta
     5161 attacttttt tgtacagaag aatcagcttc ctgtaacgaa ggcattcaaa gaattagccg
     5221 aacttattgg agacgtaaat gaatcgtact tttttgatca aatagaagtt atcgttgaca
     5281 ggatacgtaa gaataaatat aaaaatcgcc ggcctagtat tttccatcag tctctacttg
     5341 tgacgaatac tataaaggaa gaagaatcat tcacattaga attacctatt gtcgaaaaaa
     5401 acaattttga gattgaatta ccaacattgg atccttcatt gacaatggag gaaatggcct
     5461 ttcttaaacg acaaatcttt tctgagctga gcaagaaatt tataccgcat gcattttctt
     5521 atatcaaaga atatggaaca gttttagtga gtaaaaccta agagagcagt aagcaaaaat
     5581 tatactgact cttgcaggag gagcctttct cttcctttat ggttaaaaga attatttgag
     5641 tattttgcct atctaccacc tattagattc actacatggt ctggaaacaa aggaatgcaa
     5701 aaaaatggtg agaaatgtgg aatgacaaag gaagtagtga taagaaaagt tagattttta
     5761 aataatcaat attatgattc agtcaagtat ggcattttat gggaagaact agctgattag
     5821 attaggaagt ccatttgata atccagaatc actaagaaac gaatggcgag tcattaataa
     5881 tttatccaat cttgggttat tcttttagat tgaagtgttt aaaactaaaa aagcatttat
     5941 gaagtcgtaa tagtcaggct tcaaaatgct ttttattttg tttgtttttt ctttgccaca
     6001 taaatacacc tagactgacg atgaagagca tatccaacag atttcctgcg acttggatcg
     6061 tcgaagggtt ggtcagtggc attaaagcaa ggacaaaaaa ggaaaacaag ctgtaaataa
     6121 aacaggtgac aggaactaat cgaaagaaga gcggacattt caattggcta aaaatgagtg
     6181 aaaaaataaa ataagtaaaa atggataaaa tcacgactaa accattcatg ataaagaaga
     6241 aaagcgtatt gtgattaaac gaataaaaaa gaattgctaa aaaagaaaac gttttaaatg
     6301 caatcaatac gcttgaagct tcgtttaacg taccaggatg tcgatgaaaa aaataggcac
     6361 cgaaataaac agaaaacagt aagccgcaaa gagaagacaa aatcaaaaaa aacggatttt
     6421 cttcggcttc tgtgatatca gaatgatcca aaagcaacca gcctaatgca ctagaaggcg
     6481 catgtgaggt cttaaggtat gaataacata gaaaaaataa gatcgtcaaa gttgcaaaaa
     6541 aagagcctaa atgtaaaaaa ttagtggttc tacgttgaat ttccataata tctccaaatt
     6601 aaattgataa gtatataaag tataataaca aattgatgag tatatgtcga tgttttttaa
     6661 tcaaaataat aaataaaaac tgtctaacat ttaaaattta atttagatat tagacagttt
     6721 ttaagtttgt gagttatttt ccttgtcgtt ttctcacttt tttagtccag aaaccacctg
     6781 aaatatattt tggctcataa gaaatgataa aagcacgctc ttcgatttct ttgatcaggc
     6841 gatacagttc acgttcgctt tttctaggag aaagtatttc gagaactagc cgctcacctt
     6901 ccagccccat tgcagaagtc tgtgtcacac cataaccgtg ttctctcagt atacccggca
     6961 aacttttatc ttctgcgcta tttgctggca aaatcgttgt gaccatgatg tatcctaatg
     7021 ccaatttgtc ttcaatcaaa attccgacac tgatacctac tgcgtaacct aacgcataga
     7081 caaataaatt cagtggatta tctaaacgat ctaaaacaag ggataagccg acgatataaa
     7141 tcgtgatctc gaccatactg acaagtggag caatcagtcg atagcctttc attgtcaaca
     7201 tgaaacgtaa cgtgttcaga gtgatataag caaaattaat gaggaagatc atccccaaca
     7261 tttttggatc aatcatttgt aaaactcctt tcatttatat aacaacaata tcaaaaaaaa
     7321 tcgtaagtga agaaaaattg aaactttata catacaaaaa aatgataaaa tgattataat
     7381 agaattataa agataggtta ggtgattaaa atgaaaacgg tcaatcgtgt gttttgtgcc
     7441 agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat tcaacagcca
     7501 attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat tttaagcgtt
     7561 tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag ttttttgaat
     7621 ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg ttctctgtgc
     7681 ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa cctgttccag
     7741 acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat aggtgagtaa
     7801 caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt agaagttgtt
     7861 ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg atggcagaca
     7921 agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat tcgtgtgatc
     7981 ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat tccatcctat
     8041 acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat caagcattta
     8101 gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat ggggggaatg
     8161 acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg tttgatgggt
     8221 tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga atataattat
     8281 gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga tttgtcattg
     8341 cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc tgacgaaaaa
     8401 atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc ttacggaaaa
     8461 gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc actaatggaa
     8521 gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt gaaacggaaa
     8581 atcactcatg acgatagagg attttccgtt ttttattttg ggctataata aactctgtta
     8641 ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa gagagcatgc
     8701 ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc tctcgagtca
     8761 tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca gcaatggctg
     8821 aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg tcgcagatca
     8881 tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg aatttgctga
     8941 tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt acaaatgatg
     9001 ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa aaagacttcg
     9061 attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct gaaggatatg
     9121 attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat gccttagaaa
     9181 gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt gaaatttcaa
     9241 gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa atcttgcggt
     9301 ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca gattttacag
     9361 ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta agagggatta
     9421 taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat ggggtatttt
     9481 tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata cgtatgggtg
     9541 aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt caaggttatc
     9601 gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta ttactatata
     9661 tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca
     9721 atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag
     9781 aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa
     9841 ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc
     9901 agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc
     9961 gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa
    10021 agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag
    10081 gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag
    10141 agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt
    10201 ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg
    10261 aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag
    10321 aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa
    10381 caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta
    10441 tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata
    10501 gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg
    10561 cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca aat
//
LOCUS       DACDCJ010000090        10605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDCJ010000090 DACDCJ010000000
VERSION     DACDCJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.090"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            278..2224
                     /locus_tag="IV038_002420"
     CDS             278..2224
                     /locus_tag="IV038_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8342589.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2227..2682
                     /locus_tag="IV038_002421"
     CDS             2227..2682
                     /locus_tag="IV038_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8342590.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2696..4024
                     /locus_tag="IV038_002422"
     CDS             2696..4024
                     /locus_tag="IV038_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP8342591.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4058..4342
                     /locus_tag="IV038_002423"
     CDS             4058..4342
                     /locus_tag="IV038_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8342592.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4344..4859
                     /locus_tag="IV038_002424"
     CDS             4344..4859
                     /locus_tag="IV038_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP8342593.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4875..5537
                     /gene="rpe"
                     /locus_tag="IV038_002425"
     CDS             4875..5537
                     /gene="rpe"
                     /locus_tag="IV038_002425"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8342594.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5544..6116
                     /locus_tag="IV038_002426"
     CDS             5544..6116
                     /locus_tag="IV038_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8342595.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6303..7823)
                     /locus_tag="IV038_002427"
     CDS             complement(6303..7823)
                     /locus_tag="IV038_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP8342596.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8066..8251)
                     /locus_tag="IV038_002428"
     CDS             complement(8066..8251)
                     /locus_tag="IV038_002428"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP8342597.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8235..8417)
                     /locus_tag="IV038_002429"
     CDS             complement(8235..8417)
                     /locus_tag="IV038_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342598.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8658..8876
                     /locus_tag="IV038_002430"
                     /pseudo
     CDS             8658..8876
                     /locus_tag="IV038_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9371..9970
                     /locus_tag="IV038_002431"
     CDS             9371..9970
                     /locus_tag="IV038_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8342599.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10034..>10605
                     /locus_tag="IV038_002432"
     CDS             10034..>10605
                     /locus_tag="IV038_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342600.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         3502 a         1670 c         1983 g         3450 t
ORIGIN      
        1 aaatacgaat tttagtgacg ccattatatt gtttttattt ttcaattgta atatttcata
       61 ttacttccat aataaggagt attttctatt ggaatatctt agctctttta aggatttctc
      121 cttttttgtt tctcattttt taagattatt tggatttcct ttacaacaaa cttccacaag
      181 tattattgac atagatgtaa ttgagggaag ggggttccag tgctagaata aataatgaaa
      241 agaggtaaca aatgctagcg ttaagaggtg aacaagtttg attgaaagac atgtaaattt
      301 attaaaatta ttaagtcgac aagaagactt taaacccgct tcttttttta gtgataaatt
      361 ggcagtgtcg accaagacca tttatcaaga tattgaattg ttggaagatt atgtagttaa
      421 ttaccaagta acaattgagc gattaccttc tttgggtatc aaactcatag gtgataggaa
      481 aaacgttgaa catttgattt catcggtaga aaattatctt gaaaaagata tttattctcc
      541 agaaaaaaga cgtgtcgaga tcattaaaga agttttgttt ggtgataagc ctgtttcttt
      601 aactcatttg tctcaaagtt ttatggttag taaaacatcg ttatacaatg acctgagaat
      661 tatcaataaa attattggca gtggagaagt taagttgcag tcatctgcag aaggaattag
      721 ttttactggt aatgaacaag caattcaacg agcaatcaag caacttgtat tctattatgc
      781 gaatgacatg gaatcaaaaa cattttttca agttttaaat atgttgtttg attcaaaagt
      841 tattagtgtc attcatgatt tgctattcaa tgattacagt gaactgactg aaaaggtttc
      901 tgattactat gttcggtcat tgatttctac tttagttatc caatgtaacc gtttactgaa
      961 aggtcatcat ttaactgttg aagaagaatt cctcttcaat agcattcgct atatggaaac
     1021 gtacattgtg gcaaatggtt taattgaaga attgaaggca aaattgaatc ttccatttta
     1081 tgacgcagat aaagaatatc tatgtcgtca actatttgca catcgtgtga caaataattt
     1141 aaaaaccagc aatgaagagt acgctatgag cgttcataaa ttgattgaaa gaatgagtga
     1201 gattgaaaaa attgatttga ctcaaaatga tcgattatat caatccttat tgtatcatgt
     1261 gccagcaatg gtccttcgtt taaaaaaagg tattaaaatt caaaatccgt tattagaaaa
     1321 tatcaaatct caatattcag aactctttag catagtatgg tacgccatgt ccatccttga
     1381 agaagaattt gatgtgactt taacggacga tgaagtttcc ttaattttga ttcattttca
     1441 gattgctatt gaagatcagt ccaaagcaaa taatatcatc attatttgcc aatacggtat
     1501 ttcttctgcg caattaattt acaataaagt tcgacggttt ttaccagcaa aagataatgt
     1561 agaaatatca actttagata agtttcattt agctgaaatt ggggaagttg atttgattat
     1621 ttcctctctt tcctttgatg caggtggtat acccttagta agggtatctc cattggtaag
     1681 taaaacagat tatatgaata ttatggaagc ttatactaaa tatttaatag aagaaccttc
     1741 gcgatttatt caaaatgaac tgattcctaa agcggttaaa gttcccacca tatctaaatt
     1801 tgttcaatca tctttagtaa aaacaggagt acaggtggaa agcaaagaag aatgtttgga
     1861 tttgatgatt tccgaactag aagccaattc attagttact gaagagtttc gtgcttctat
     1921 cttctctaga gagaaaatgg ggaatactaa tttagaaagt ggtgttgctt taccgcatgc
     1981 gaatccatca atggtcattg aatccaacat ttttattgta gcattaaaaa atgcgattaa
     2041 ttggggcgat cgaaaagtca aattagtcat tatggttagt ttagctgaag aagatgtatc
     2101 tgagattaga ggagtatatg aagaacttta ccaatttatt gggcaaaaag aaatggtcga
     2161 tcgcttgtta caagttcatg atagcgaaaa attactaaga atttttcaac aaaaaggaga
     2221 ataaaaatgt atctagataa agaattatgt ttatttcaat tagaagtgaa agatcaaaaa
     2281 gaattgtttc aagtaatgtc tgaacaatta aaaaacgctg gttgcgtgaa agatagtttc
     2341 ttagaaggta tcaccaaccg tgaacaagaa tttccaactg gattagaagt aaatcaaatt
     2401 gggtttgcta tcccacatac agattccgca catgtaaata gctctcaaat ctgttttgcc
     2461 tcattaaagg aaccactggt attttcagat atgacagata aatctcatga aatacctgta
     2521 cgtttagtct ttatgttggc aatgtcacaa cctcatgaac aaattgatac actacaaaac
     2581 ttagttagcc tatttcaaaa tgaagaaaaa gtaaatgaat tacttgcatg taccacgaaa
     2641 gaagcattta ttgagttagt acattctgca ggagtttatt aggaggaata gagagatgga
     2701 tattgtatta aatgcctttg aatggtttat tggattaggg tcaaacgtct ttttaccact
     2761 gattattttt gtaatcggga tattatttgg attaaaacct ggtaaagcat ttgtttccgg
     2821 cgttactgtt ggtatcggtt ctattggttt aggattagtt ttaagtttat tatcagatgg
     2881 tttaggaaca gcaattcaac aaatggggga acaatacggt ggtggcctga atatcctaga
     2941 tattggcgta ggtgttggtg gacctttggc ttactctaca tcattgggaa ttttaatgat
     3001 tccaatttca ttaattctta acttcatatt tgtggttttg ggttggacaa aaacattgaa
     3061 tgttgatatt tggaactttt ggttcgcaat tttcctaggt atgattactc aatctgttac
     3121 gggaaacttt tggtttggct taattggcgc gattgtcgca gtaattttac aatggttctt
     3181 agcagatcgt acacaaaaaa ctgttagcga atttttcggc tatccaggaa tctctatcac
     3241 acatatgatg ggattatcag gagtactatt tgccaaacca gtaaattggg tttttgatcg
     3301 tattcctgga tttaataaaa tagatgcaga tgcagaaaca ttaacaaaac gtttcggtgt
     3361 tttaggtgac acagttgtta ttggcctaat tatcggtatt gtagtgggta ttatggcagg
     3421 ctatgacgta gcaggaattg gaaacttagg aattatgact gcggcagtga tgaagattat
     3481 gcctaaaatg gtagcaatgt ttatggaagg tttaatgcca attgcagaag cggcgaaaga
     3541 atttacgaat aaaaagttaa atggtcgcca agtaaacatt ggaatggatg cagcgttaac
     3601 tgtgggtcat ccaactatta tgtcaacttc tctattgctt gtaccaatct cattattgct
     3661 agcactaatt cttccaggaa ataaagtatt accttttgga gatttagcat tctttgcttt
     3721 cgccatctgt ttaatgattc cttattttaa aggaaatatt gtgagatcat tagtaggatg
     3781 ttcgttgtat gttgttacaa cattatatat ggcaacatgg ttggcaccaa ttatcactga
     3841 tgtgtttaca ttagcaagtt tcgatgtggg gacagcagga ttagtaacta tgttgttatg
     3901 tggattatgg ccagtagggt tgtttgtttt attagcatct tactttggtg taattggaat
     3961 aggtggatta ggagttgttt tagtaggtgc ttctatctac atgaacaaag tgaagaaagc
     4021 ttaatcataa aattaaaata agaaagaaga gatcaatatg aaaaaattat tagttatttg
     4081 tggagcaggt catgctactt ctacaattgc agtagcaaaa ttatcaaaat ggttggaaga
     4141 agaaggatta acagatcaag tgaaaattta tcaatctaaa attgctgatg agctaaataa
     4201 aattgatgaa tatgatgctg tagtaagtac aacaattgtc cctgatgtaa ttaaagctaa
     4261 agtaacgaat ggcttgggct tattaacagg tattggtaca gatgccattt atgcagatgt
     4321 aaaagaaaga ttaggtgttt aatatgcgtt ttcatcatga cactttgact attgaaacag
     4381 tttcagggcg tccttcgtat catgatattc gaaaagaact tttagataca gttgatacag
     4441 caggtattga gaatggaatg ttagtaattt cgagcccgca tacaacttgc tcactctttt
     4501 ttgaagaaac gatgcatgac acaaactttt ttggtgatga ttatttacat gtagacatca
     4561 acaatgtaat ggaaacgatt attccaaaaa tgacgagtga aaatcaatac aacagtccgg
     4621 ggccaaaaca cattgatttt ggaacaagat tatctgatcc caattaccct gcagaaaaat
     4681 gggtcatgct aaataccgat gcacatttac gttcatctat ttatggaacc aactcaatga
     4741 ctttaattgt tcaagaaggt aaacttttat taggagaaat gggccgtgtc tattttgtcg
     4801 attgggatca actaagagag cgaactagaa caatcaatct catgattatg ggagaataaa
     4861 cgggggaata gagaatgaca attgctccat cattgttaaa tgcacctatc gcagaagtta
     4921 aagagttatt tgaaatctta aaagaaaaga atgttgatta tttgcatatt gatgtaatgg
     4981 atggctgttt tgtaccagat atggcttttg gaccaagtgt tattaaagat attcgtccac
     5041 taacagacgt tgtgtttgat gttcatttga tgattgatac accagaaaaa attgtcacaa
     5101 gatttgtaga agcaggagca gatattgtta ccttccacat tgaagctacc aaacacgcaa
     5161 tgaaggtcat tcagttagtc aaggaagcag gagcaaaagt agggattact tttaatcctg
     5221 caacaccagt cagttttatt gaaccagtat tatctttagt cgatcaagta ttagtaatga
     5281 caatcaatcc aggaacagag aataagcatt ttattcaaga gacagtagtt aaaatcgaac
     5341 agttagatgt aatacgcaaa caaaatgatt atacgtatga tattgaagta gatggcaaga
     5401 ttgataatca aacgattaaa gtttgttcta aagcaggggc ggatattttt gtatcaggcg
     5461 gtttcatctt caatgggaaa aataccccgc aagaacaaat tgataaacta aatcaatcat
     5521 tagaggaagt gaagtaaaag atcatgacca ataaagacca aaataaaaac tatttagaag
     5581 cgattatctt agaattacaa aataactcaa aacgtattaa ttctgtagag ttagaacaat
     5641 ttagtgagaa aattgttcaa gctaaacaca tctttctagc tggtgcagga cgctctggaa
     5701 ttgccatgca agcatttacg aatcgcttaa tgcatttagg tttttcagtc agccttgtcg
     5761 gtgaaattag ttcaccacat tcgcaacctg gagatttatt aattatctgt tctggctcag
     5821 gtgaaacagg tagtttaaaa aatttagcgg ccaaagcaaa tcaaagtggt gtaagcattg
     5881 ctttagttac catgaaaaaa gactcgtcga ttggtcaatt agcagattct gttcttgtat
     5941 taccaggtat taccaaagat gaaaatcaac gagaagaaga tactttttca caacctatgg
     6001 gttcaagttt tgaacaatta gcatttctca cctttgatag tttagtgtta tctttaatgg
     6061 ataaaacagg tgaaagctca gaaacgatgt ttgctcgaca tgcagatttt gagtagtaga
     6121 ttttaaagat aaaaaaccat tctctaaatg ctaattattc agcaaatatg agaatggttt
     6181 ttttatttga ttagacataa cttaataatt cagtagtttt gagcgtaagc gtgtcataga
     6241 atggtaccta aacacaaata gcccgatttg atacactcaa atcaggttat ttgtggctta
     6301 tattatttac agatttcttg tatctgctga ttccatggca aataagcgcg tagcgcttca
     6361 ggtgtcatcg aagacagatt gggaatctct gtaaaaagat gatttaaata tttgcggatg
     6421 tttaacccat tcgcaacagc tgtctggata atactcaata taattccatt tgatctagcg
     6481 ccttttaagc ttttagaaaa tagccagttt tttcgaccga tcactaactc tttgatggca
     6541 cgctctgctc ggttatttga aagttccaag cgtccatcaa gcaacacaat ctgaagtccg
     6601 tctttatgat tgactgcgta ttgaaccgcc tttccaagct gcgattttgg caaataagca
     6661 aaactctcac accaatgaaa aaattcagtc atcacaggct gcaacgtttc cttcctcgct
     6721 acaaaccttt cttctgcgct aagcttatta aaggtccgtt cgattcgaaa catcttgtca
     6781 cagaagtcta atcctatttt ggcatgactt tttggggacg gttgtttacc caatgcttca
     6841 aaaaactttc tgcgaacatg cgcccaacaa taaacattgt ttaccggcag ctttccatag
     6901 gccgcgtaac catcagtatg caagaaaccg ctaaatgatt ctaaaaaact ttttggctca
     6961 tcaacggaac gcgaatttgc gtgctcgtag agcacaactt gtttttcttc gcttttacct
     7021 gaagcatata accagtaaaa ggtcttttct ttatgagtat tcaatacccg atagcttgtc
     7081 tcatccgcgt gtaagacctc ctgattgctt aattcttgat gaaggcaatt gtagattggc
     7141 tctaaaacca tttcacttac cttgatatgc caattagtga tttttttacg atcaagaggg
     7201 aaaccaagtg catgccattc tttttcttgt cgatatgccg gcaccttttt aacaaatttt
     7261 tcccaaatac tctgtgtcac aattgaagag gaacctagac tattattgat caacggttta
     7321 ggaacaggcg cttttttgat ttttgtttct cctaatgctt cacattctct gcaatgatag
     7381 gcatgttggt agatgacttg tttttctata tgggcaggaa catacaatgg ctcttctcta
     7441 agccttgtcg caccgatatc atgtaaagta tgaccacagt cttcacacaa acaatcggca
     7501 ccttttaatt cataatgaat aggaacttca ggcagacctt ctaatgaaag tgctttgagt
     7561 cccttgcgtt ttctgcgttt aaaagaagat accgtaatgg gttcctggct ttcttgtcca
     7621 gtgtgctctg gttcattaaa agttgagtcg tctaataaat cgatttgccc atctacgggt
     7681 ggtgtttctt tttttcgtcc aaacaagcgt cgattcattt cagcaatttg ttctctaaga
     7741 agagtatttt cctgttgctg gatttcaagc aactgtgtta attgcttgat ttgtacaaga
     7801 agttcagatt ccatttttgt catttttaca ccaccattct agcgattttc ttacatctat
     7861 tataccattt tatgacaacg aaaaacagac aaaatccttg atttaacaag gttttcgtct
     7921 aatatatcgc accttttttc gtttctttta gcgtacattt ttcttcgatt gaaaggccag
     7981 tcagtaagac ttcttttctg gaacgcggcc attgaaggta cccactttca aatcttttgt
     8041 acattaatac aaatccattt tgatcctagt aaagagcctt gaagcgatct ttcttcgtac
     8101 cgcaaaataa gaaaagcgct tgatcgtaaa catcaaggtc atattcttct ataacgaaag
     8161 ctgctagtcc atcaattcct tttcgaagat ctgtttttcc acaaacgata aaaatatgtt
     8221 tcaatgcagt tgcatcaatg agcattggca ttcaactctt ttaacaaggt atacaaaaga
     8281 tatttatcca ttccattata aataaatatt tccttatctt ccgatttgat tctgcaagct
     8341 agttttggct tggtgtcaat ttgcgatttt tgagccgcac ttgaaaattg aactggtaca
     8401 atatcttttt tattcattcg tttttctcct tctatatttg atagtttaat tatagaagaa
     8461 agataatcgg cgtacgagat actatcctat gacacgctta catttaattt aatattttgc
     8521 ctcaaattag ccacactttt tatagaacta agaggaaaaa tgaagttttc ttcctattaa
     8581 tatagttgtt tgaaatctta aatatagcgt tttacgatgt aatgggaagt atggtgaata
     8641 aatcaactat gttcgtacga agcatttact caactaattt aatcgaatct ttcaataaac
     8701 aaatcaagaa atacagccac cgcaaggaac agttccaaaa tgaagagtcg atggaacgtt
     8761 tcttagtctc gtcttttgat acttacaacc aaaaattcct aggtcgcagt cataaaggtt
     8821 tccaacaggc cgaaggcgag ctcgaacaaa tgctgagtca accgatggag aattagaaac
     8881 aatctacagt agggaagaac cattgacaca aaattattga cgctcccaac taaaagtgtc
     8941 aaaataatga tgaaatcatt tagttcttat aaattagaaa agagaaaata aatattccat
     9001 atctacttta ttggatccgt aacatgacta aattctgaat actttttaaa gcttatcatt
     9061 tggtaaaact gaatataagt taattatata gactgaaaat tttgaatttt cagaatagaa
     9121 taacttaaaa gtactgacaa aattttttaa aggggcaata ttaatgttct gagataatca
     9181 aatcctaccg aaaatatcaa aaatccaatg tgacacaatc ggattgtgtc tcattcgagg
     9241 gaatcacaca atcccacgtg gctctaggca gaattgaatt tttgcttggg gctttttcct
     9301 tatattgatt ttgaatgaga cttattatat gttataatgc aattaagtat cattggagag
     9361 ggcgaagaaa atgaagacgg tagaacctat tcgagacaaa aaaaagatcg atgcgatgaa
     9421 ggcaatcttg gcttcgggaa aatatggcca acgaaatttg gttctttttt cgattgggat
     9481 caacaccgcc tatcgcattt ctgatttgcg tcaattgaag ctggccgacg tgctggaaat
     9541 ttcacgcggt cgcgtggtgg tcaaagatcg attagcgatg aaggaacaaa aaacagctaa
     9601 acataattcg gtctatattt ccaacaaatt gcgaaaaacg attttggagt acgttcaaca
     9661 ggaatttcct gatcaactcc ttcagcaaga ttttgacggt tatttgttcc ctagtcgaaa
     9721 agggacgaat gaaccgctca accgccaaag tctgtggcga atcattcatg atgctggcac
     9781 ggcaatcgga ttgaaagaca ttggcccaca ttccatgcga aaaacgttcg gttactttct
     9841 ctataaacag gggaccaaaa cagaaatcat tcagtccttg ttgaaccatt cttctcagcg
     9901 cgaaacattg cggtatattg gtattacaca agaagacaaa gatacagctg taaaaagctt
     9961 agatttataa tataaaagtt aagtttaaac atggatagtt ttaaattagt agattgagtg
    10021 ttgggaggaa agaatgagta cattatatat tataggaaac ggttttgatt taagtcatgg
    10081 tttaaaaact agatacactg atttttcaaa atgggttgaa aaaagtcata gtgaaatatt
    10141 cgataaaata gacacaatga taaatgaaaa taatgaggtg agaagtacag ctgatgataa
    10201 gataggttgg tttaattttg aaagtagttt gcatgagtta attcaaagtc cagtctttga
    10261 agattatgta gaagagtcta aggattatct cataggttat ggacatgaag attggtcaga
    10321 ttctgcccat catgattttc aaaatatggt ttatgattat gttgtttctc tagaagaatt
    10381 acaacaactt ttcaaggaat ggatacgcaa agaaatacag ccgacaatag aacataaaat
    10441 tccaaaatac caattttcgt ccgatgtact attctttaca ttcaattata cagatgtttt
    10501 ggaaattcta tacaagcaac cgaaagaaaa catatatcat attcatggca gttatgacga
    10561 tataataatc ggccactccg agaattttga caactttata ggaga
//
LOCUS       DACDCJ010000091        10473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDCJ010000091 DACDCJ010000000
VERSION     DACDCJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.091"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IV038_002433"
     CDS             23..646
                     /locus_tag="IV038_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342601.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IV038_002434"
     CDS             1096..2145
                     /locus_tag="IV038_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8342602.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IV038_002435"
     CDS             2142..3023
                     /locus_tag="IV038_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8342603.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IV038_002436"
     CDS             3026..3796
                     /locus_tag="IV038_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8342604.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IV038_002437"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IV038_002437"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP8342605.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IV038_002438"
     CDS             5107..6270
                     /locus_tag="IV038_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP8342606.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IV038_002439"
     CDS             6387..6797
                     /locus_tag="IV038_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP8342607.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IV038_002440"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IV038_002440"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8342608.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IV038_002441"
     CDS             9337..9654
                     /locus_tag="IV038_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342609.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IV038_002442"
     CDS             9814..10050
                     /locus_tag="IV038_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8342610.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IV038_002443"
     CDS             10047..10445
                     /locus_tag="IV038_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP8342611.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3648 a         1763 c         2221 g         2841 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa tta
//
LOCUS       DACDCJ010000092         9873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDCJ010000092 DACDCJ010000000
VERSION     DACDCJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.093"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(96..2216)
                     /locus_tag="IV038_002444"
     CDS             complement(96..2216)
                     /locus_tag="IV038_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8342612.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2446..3624
                     /locus_tag="IV038_002445"
     CDS             2446..3624
                     /locus_tag="IV038_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP8342613.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3640..4398
                     /gene="aroD"
                     /locus_tag="IV038_002446"
     CDS             3640..4398
                     /gene="aroD"
                     /locus_tag="IV038_002446"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP8342614.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4421..4906
                     /gene="ybaK"
                     /locus_tag="IV038_002447"
     CDS             4421..4906
                     /gene="ybaK"
                     /locus_tag="IV038_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP8342615.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4977..6230)
                     /locus_tag="IV038_002448"
     CDS             complement(4977..6230)
                     /locus_tag="IV038_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8342616.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6347..7696
                     /gene="gdhA"
                     /locus_tag="IV038_002449"
     CDS             6347..7696
                     /gene="gdhA"
                     /locus_tag="IV038_002449"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP8342617.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7808..9154
                     /locus_tag="IV038_002450"
     CDS             7808..9154
                     /locus_tag="IV038_002450"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP8342618.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9462..9848)
                     /locus_tag="IV038_002451"
     CDS             complement(9462..9848)
                     /locus_tag="IV038_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP8342619.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3148 a         1793 c         2023 g         2909 t
ORIGIN      
        1 ttctattacc gtagatgcaa gcttttgaac actgtttatt cgaaaagagg agcagcgaca
       61 ataattttgt cgctgctcct tttaaattta ttttatcatt cggaagaact tgtggtcgtt
      121 ccgtgaagtt cttggtacgt ttgcgtttta taaagatcaa cagttgattg tccgttgtgt
      181 tcactaaaga cactagttga cttgtcacca agagattttt cagcttcttt taatttctct
      241 aagccattct tgtagtcata atcactggta tcaattgggt ctaatccact attcgtatag
      301 aaccgaagaa gatcaccatt attgatttgg tcagaaacag ctaactgttt atcaactttt
      361 tctttcagtg aatcaatttc attctgtact tcctcagtag gttcagtaat agcttcacca
      421 gtttcgttac tatagatcgt accaccgtag taagtatatt ttggtgtaac aaaatcacca
      481 tcacggaaag aaacaagctg ttcatgatct ttcgagaata gatcttgtcc taactgaata
      541 taattttttg tatctacacc tagtaagtga agtaatgtag gcaaggcatc aacttgtccg
      601 ccgtaagtgt gattgattcc gccctgtgtt tgtccaggga tatggatcat ataagggaca
      661 cgctgcattt gtgcattgtc gtagtttgtc caagtagaac ttgtttttcc aactaattca
      721 gctagatttt tatttcttga attcgagaca ccgtaatggt caccgtaaag gacgatgact
      781 gaattatcat acaagccgct ttctttcaaa taattgaaga attcttgtac ggcggtatct
      841 aaataattgg ctgtcgcaaa gtaaccgttg atcgtttcat catttgtatt ggctaacggg
      901 aatcctgctt cgtcattggt gaattttgaa taaggataat ggttagatac agcaatgaat
      961 tttgaataaa atggttgttg cagacgttcc aagtattgta cggattgttg gaaaaatggt
     1021 ttatcatgca acccgtattg gaacgaattg tcatcattaa catcataata agaagaatca
     1081 aagaaataat catatcctaa attcttgtat gtctcgtttc gattccagaa tgtaccagcg
     1141 ttcccatgga ataccgcact aatgtaacct tgtgtttgac ttaaaatatc aggtgcagct
     1201 tggaaagtat tttttccgcc taattgtgtg aacaaggagc cttgattcaa gccaaacaat
     1261 gaattttcca tcagcgtttc cgcatcgctt gtttttcctg cttttacttg atggaaaaag
     1321 ttgtcaaaac tgaacgtgct gttgctgtgg taaagactat tgatgaaagg tgtaacttca
     1381 tgctggagac cattctcatc tgtcaattta taatcgatca ggaattgctg gaagctttct
     1441 aaatggatat aaatcacatt ttttcctcta gcgagcccga acaaatcgtc atttggtgct
     1501 gcataatgtt cttttacata attttctact tctttcatat cattgggact tgcttctgcc
     1561 cgcacttggt ttgtctggta agtttgaaca ccgtcataaa ccatgaaagc attcaagcca
     1621 agatatttga ccaaataatc acgagagaat gttcgagtca gcaattctgg acgatcagct
     1681 tccgccataa aaagatttcc tgagaaaatc ataactgcta atgtagagat cgcaaaagcc
     1741 attcttgctc ggatcgggcg agggtccaac ttgatttttt ttgcaatcag cagaacagtc
     1801 aagataataa aatcgatcca atagatcaca tcatatgggc gaaacagtcg aatggcactt
     1861 tctcccagac cactcgctac tttcccagca cctaaaatcg tgttgatcgt aatgaagtcc
     1921 gtgaattctc gataataagt gacattcgaa aaaacaagca atgtcaaaag aaaataaatg
     1981 accatcatcg tgatataagc aactctaggg cgtctgatat aaagagcaat tccaagtaat
     2041 aacaatgtag ttgcaatagg gttgataaac aagataaagt attgaatgcc actttcgatt
     2101 ccgagatgga aatcaactaa ataggcaaaa atcgttttca accaaagtaa aacgactagt
     2161 aaagaaaaaa atccgagcct cttattcaaa aaggcaggtg tttttataca tttcaagata
     2221 atcttccttt ctgcacttct taatcacaga agtgtcctga aaccatgtta cacagctcac
     2281 ttattctcat ttattttact agtatgacaa tatagtgtca attgaaacca gaagtttatg
     2341 ctgtttcatt aaggttttct ttaagcttca tgaaaaaatg agcagtaaga caagtcctat
     2401 gttttttgtt atacttggga agagaaagct aaagcaggtg aaggaatgaa agtaaaagta
     2461 aaaaaaagag caagcaatcg attgaaaaaa ggatatccac tgatccaaaa agaagacctt
     2521 gctcatcccc aaaaagaaac cacgcaatgg gtggaattag ttgattcgca aaaccaattt
     2581 atcgcaaaag gatatctagg aaagcagaat aagggaatcg gttgggtatt gagccagcag
     2641 aacatcccat ttgatcaatc tttttttgaa gaattttttg tgaagtcaaa agaaaaaaga
     2701 actttctttt tttcagatga ttctactacc gcttttcgta tattcaatgg agaaggagac
     2761 gggatcggcg gtctgattgt tgattattat gcaggttatg ctgttttttc ttggtataac
     2821 gaaacgttat atgcacatcg tgaattattg attcaagctt ttcgtgaaat ctacccggaa
     2881 atcataggag gctatgagaa aattcgattt gaatcagtaa atctgcctga gagccagttc
     2941 atttacggaa aagaggcacc ggaacccttg atcgtaaaag aaaacggagt aaaatttgcc
     3001 gtttatttaa atgaaggatt aatgactggg atctttttag accaaaaaga agtacgggga
     3061 tcattagcag aaggtttcgc ttctgggaaa acagttttga acatgtttag ttataccggc
     3121 gccttttctg ttgctgccgc aatgggaggg gcaatcgaaa caacaagtgt cgatctagca
     3181 aaacgcagtt taaaaaaaac acaggaacag tttgaagtga atggtttgga tgtcgatcag
     3241 caaaaaatca ttgttatgga cgtattcgag tatttcaaat acgccaaaag aaaacaattt
     3301 ttatatgaca tgattgtatt ggatccacca agttttgcca gaaataagaa aaaagtcttt
     3361 cgcgtagcaa aagattatgg ggaactggtc aaggatagtc tggatatttt agcagaagac
     3421 gggatactta ttgcgtctac aaatgctgcg aatgtctcga tagaacaatt ccaaacaatg
     3481 attgaagagg aattccatgc caaaaatgtt tcttttgaac aacttgcttt atatcgtctg
     3541 ccaaaagatt tccagacgac ggataccttt ttagaaggca attatctgaa agtgtttgtt
     3601 tatcaaatta aaaataacca ttagaaggag cgatgcagaa tggttcgggt aaagggattg
     3661 aatataggaa gcggtagacc aaagatttgt gcgcctctta tcggggccgt ccgagaggaa
     3721 gtattacgtg aagcagttct tgcacgtgaa gcaggggccg atttagtcga atggcgagtc
     3781 gatcattatc gtgaagtttt ttcttttgat gaagtagcag aaacattggt tcttatccat
     3841 gagatgcttg atggactgcc gttattattt acgtttcgta ccttagctga aggcggagaa
     3901 caagacatat cgatccgtca gtatcgcgac ctttatgaac atgtgataca cacaggatta
     3961 gtagatatgg tcgatatcga gttgttcaaa gtagaaagtc taggaaaaag actgatagag
     4021 gaaatcaagt ctttaaacat tcctttaatt atcagtagtc atgattttaa agaaacacca
     4081 gcagaccctg tcttgcttta ccgattgaat gttatggaac attttggagc cgatatcgga
     4141 aaactggcag taactcccaa taatgaacga gatgttttgc ggttgatgga gttgactaga
     4201 cgggcaaatg cttttgtctc catgcctatt attacgatgt ccatgggaaa tcttggtaaa
     4261 atttctcgtc tggttggcgg aacgacgggt tccgttatga catttggttc cttaagcagc
     4321 gattctactt ctgctccagg acaattaccg gtcaaagcat tgaagcaaat tattcaatta
     4381 ttaaatgaac cgttgtagaa caagtttgag gtgatatttt ttggcaaaga aaaaaatagt
     4441 aaaaacgaat gcaatccgta tggtcgaaca aaaaaagatt ccctatacag agcacgaata
     4501 tgagtgggac gaaagccacc taagtgcgtc atcggtcgct gaacagcttc cagaaagcca
     4561 atctcgtata tttaaaacac tggttgctgt gggcaatgtg acggggcctc ttgttgctgt
     4621 gataccggga gaagccgaat tgaatctaaa aaaattagca aaagtttcag ggaataaaaa
     4681 agtagaaatg ctccatttaa aagatttgga agcaacgacc ggatacatac gtggtggctg
     4741 ctcgcctata ggtatgaaaa aactatttcc tacttattta gatcaaattg cagaaagcta
     4801 tgaacaaatc attgtctcgg ctggcagaag agggctacag atggagttag cacctcaaga
     4861 tatctgtgca ttaacgagcg gacaatttgc agatataaaa caataaatca tacgtataaa
     4921 agaaagaagg aggccgggat gttgtttgtc cagtctcctt ctttcttttt tatacattat
     4981 gctctttgcc cggcattcat tcctgctaca tgccctgtag cgaaagccgc tgtgatatta
     5041 aagccgccag tataaccatt gacatccagt aattctcccg caaaatacag accatcaatg
     5101 attttactct ccaatgtttt aggataaatt tcctttagag aaacccctcc gccagttaca
     5161 aatgatttct caataggaaa agttttgttg attgatagct taaactcttt gcatagttga
     5221 acaaaatctt gaatttgttt ttgagaggct tgttgtcctg tcagatgatc catttcaagc
     5281 cgctcaagaa agaaaactaa caatcgctca ggcaatatac catgccaagc attgaccaag
     5341 ttctttttgg ttgctttact tttttctgtc aatatatgaa taagctcttg tttggtttgt
     5401 gtagggaaac aatccagaga aagtgtcact ggttctcccg ttttctctaa ttctttattg
     5461 acaaaacttg aacaacgcag tgcagccgga ccagaaatac cgaaatgggt gaaaagtaaa
     5521 tccatcgtat gttcagtcaa tacacgacct tttttattta aaacgcgtaa agtgatgtct
     5581 tgtaaagaaa gcccttgaag tgttttctcc tgaatatacg gttcatccga tatcaagggg
     5641 gactctgtcg cataaagagg cgtcaccgtg tgacccactc gcttcacgat cttgtaccca
     5701 tctccagtag aaccagtaga tggataagtc cgtccaccag ttgttaagat aatggcagga
     5761 gcatggaact cttcgaattc cgtgcgtata ccgtaaattt tatgttcttt gtggatcaat
     5821 ttctctactc gagtagagaa tagaagcgtc acatctagtt ctttcagacg attgagcaat
     5881 gcttcgataa tcgtttttga tttatttgtt acaggaaaca tccgtccatg atcctcttct
     5941 tttaaatgta cgccttgtga ttcaaagaag ttcatgatat cgaaattatt ccattgcgca
     6001 aacgtactat ataaaaactt tccatttcca ggaatatgtg caatcaaatc atcaacagga
     6061 cgattgttcg taacattgca ccgaccacca cctgtcatca gtagtttttt tcctgctttt
     6121 ttgttttttt cgatcaagag tacgcgtgcg ccttgttcag ctgcactgat cgctgccatc
     6181 atcccgcttg ttccagcgcc gactacgatc acatcataca gtttttccat tccctcacct
     6241 caaggaaagt gtagcatatc tgaagaaaga aaacagaaaa aaatgttttg agccaaaaga
     6301 gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga caaacgcaac
     6361 agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc aagaagaatt
     6421 tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca ctcatcccga
     6481 atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta ttcaatttcg
     6541 agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc gtatccaata
     6601 caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg tcaatctaag
     6661 tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg gtctgccaat
     6721 cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata atgagatcat
     6781 gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat cgattgatgt
     6841 tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg gcgaatataa
     6901 acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt tttggggaag
     6961 taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac atctattgaa
     7021 cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc
     7081 catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat gttcagattc
     7141 tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga
     7201 aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta tttatcatgc
     7261 tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat gtgctactca
     7321 aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca aaatcgtagc
     7381 agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg aagcaggcgt
     7441 gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg ctttagaaat
     7501 gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc agctggatca
     7561 aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa
     7621 aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag caatggcagc
     7681 agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt ttgtttattt
     7741 gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc tataaggagg
     7801 agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg tcaacgaaca
     7861 agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac gggaaggtac
     7921 tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg acaaagaaga
     7981 atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag tattagttgt
     8041 tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct tgaatcacac
     8101 attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct ttgccggaaa
     8161 ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc gcgacttttc
     8221 agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt tccgtgtatt
     8281 caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta tctacgctac
     8341 aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt gggaaacttt
     8401 cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg gtttgttacc
     8461 aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa
     8521 agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag caatgcgcaa
     8581 cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac caggaatgca
     8641 atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag accaaaaagg
     8701 catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac aattcatcca
     8761 agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat
     8821 cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag gaaaagaagt
     8881 ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag acggtgacgt
     8941 gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt atacaatgta
     9001 cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc cattcgacca
     9061 accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac caggctttga
     9121 agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc tagatttaaa
     9181 taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt taggtactca
     9241 aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt cacagtccct
     9301 tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga ctttaagaac
     9361 agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca gtaagcaaaa
     9421 aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag gcctaatttt
     9481 tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat gatccgcttt
     9541 tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt ggcagagtca
     9601 ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc ataagcatct
     9661 acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat atcagtttct
     9721 gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt agactgatag
     9781 tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa ccctaaaatt
     9841 tttttcatga aagatctcct tttttatttt tat
//
LOCUS       DACDCJ010000093         9822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDCJ010000093 DACDCJ010000000
VERSION     DACDCJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.094"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            113..892
                     /locus_tag="IV038_002452"
     CDS             113..892
                     /locus_tag="IV038_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342620.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            933..1877
                     /locus_tag="IV038_002453"
     CDS             933..1877
                     /locus_tag="IV038_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8342621.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1893..2672
                     /locus_tag="IV038_002454"
     CDS             1893..2672
                     /locus_tag="IV038_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP8342622.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2685..3383
                     /locus_tag="IV038_002455"
     CDS             2685..3383
                     /locus_tag="IV038_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP8342623.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3411..4175
                     /locus_tag="IV038_002456"
     CDS             3411..4175
                     /locus_tag="IV038_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP8342624.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            4188..6005
                     /locus_tag="IV038_002457"
     CDS             4188..6005
                     /locus_tag="IV038_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8342625.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6019..7242
                     /locus_tag="IV038_002458"
     CDS             6019..7242
                     /locus_tag="IV038_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAP8342626.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            7256..7990
                     /locus_tag="IV038_002459"
     CDS             7256..7990
                     /locus_tag="IV038_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP8342627.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            8007..8618
                     /locus_tag="IV038_002460"
     CDS             8007..8618
                     /locus_tag="IV038_002460"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8342628.1"
                     /translation="MVIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRS
                     YTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNIL
                     MRLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIAL
                     ETDALNNDVVNNFYKKNEFILERKYSTNEGRIMNEYRFYMEKN"
     gene            8621..9766
                     /locus_tag="IV038_002461"
     CDS             8621..9766
                     /locus_tag="IV038_002461"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8342629.1"
                     /translation="MKLLYILNVANKVNNFSKASMMAAKQTGLEFYIAGNWKYKSDEE
                     RKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRI
                     AGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
BASE COUNT         3513 a         1586 c         1995 g         2728 t
ORIGIN      
        1 atatttctat gaatatatta gtttaattat gcttttaaaa taaaaagcgc tcattctgat
       61 gtgaatgaat cacatcttta tttatctatc atcaatattt aggagtgaat ctatggaaaa
      121 gccgttcgtc aagttattgg ctactgtggc ggtaggcaca ggcgctattg tgatttgctt
      181 gtttggatac catctcaata atcagcggca acatcatcaa cggatcaatt acgcagaatc
      241 tgccatcacc aatcaaaaag acaccgtgac ctctttatca aaagaagtag ataagctgta
      301 cagtacaaaa gaaaaaattt tcctaaatcc tgaaataaca gaagaaacag tttcaaattt
      361 gtcacataaa ctatcttcta tcaaactttc agcagatgat tatgacatca aggaatcaga
      421 attacctaaa gaagctgctg cgatccaaga agaaaagaaa gcagtcttaa cacagttaga
      481 ggatgcagaa tcaaaactga agatccaaac ggctgtcaat aaattattta ctaaaaacgt
      541 atcaaactgg cagcaagctg tggatgatgt catcatcaaa gaaaagttgg caagtgcaga
      601 tgtcgcccat gtacgtgaaa atatgagctt ttttaaggat agtgcttgga aaacggtcgt
      661 tatgcaatat ctaggattcg ctgataccca aattgcacaa gtcacgcaac tagatcagct
      721 attcgatacg atgctgaaag acggtcaagt aacagctact gctacttacg atcaatattt
      781 gaccgcactc agtctcatcg aacagatccg gaatgaaaaa ataagagccg catacgcgtc
      841 aaaagcagaa acagtcgctc agcaaatggg ctatagcaat acgagctatt aataagcgag
      901 aattcaagca aggctagcaa acggaggaaa ctatggcaaa acgaagaaga agatcaagag
      961 ggaaaaagat atttctaatt attttagcaa ttcttgtcgt attagtatta gcaatcgtag
     1021 gagtaggagc aaaattatat ctagatctct cacattctat tgaaaaaact tacgaatcag
     1081 tggaacgaac ccaaggagaa cgaacaaaac cagttgatct ggacaaacaa gaatctttct
     1141 ctgttctatt gatggggatt gatacgggag atttaggtcg ggtcgatcaa ggaagatcag
     1201 atacgatgat ggttgccaca gtcagtcctg aagacaaaca gacaacgatt gtcagcattc
     1261 ctcgtgatac gtatgtcgac atcgttggac atgacacaaa agacaagatc aaccacgctt
     1321 acgcattcgg cggagctgcc atggcgatgg ataccgtaca aaaatatgta gacatcccaa
     1381 tcgatcacta tgtttcaatc aatatgaaag gtctaaaaga actcgttgat gcagttggtg
     1441 gtatcgaagt caataatgac ctcaccttct cacaagatgg ctatgatttt acgatcggta
     1501 aaatcacatt gaacggggat caggcattgt cttattctcg tatgcgttat gaagatccaa
     1561 atggtgacta cggcagacaa gaacgtcaaa gaaagatcgt agaaggtgta gcgaaaaaag
     1621 tgttgagtct gaacggtgta agcaactatc aatccgtttt gaatgctcta gagtcgaata
     1681 tgaaaacaga catgagcttt gatgaaatga aaaagattgc tttagattat cgggatgcat
     1741 ttggtacgat caagcaagat cagctgcaag gagaaggctt catgcaagac aatatctcat
     1801 accaaaaagt cagcgatcaa gaacttgatc gtgtgaaaaa agaattaaaa gatcaaatga
     1861 atctagagaa taaataggaa agtggagaaa aaatggaaga gacaatcagc ttgaaagaaa
     1921 tttttgaaac attacgcaaa cacgtgataa caatcctcat ctcaatgttt gctggcttgg
     1981 ccatctcagg catcgtgacg tttttcatct tgacgccaaa atacagctca caagcccaat
     2041 tgatcgtgac actgccgcaa tcagatactg caaatgtcaa tgatgtcaat acgaacctgc
     2101 aaatgatcaa tacgtataag gatatgatcg tcagtgatct agtactaaat gaagtcaaag
     2161 atcgtttaga aacagaagac aatgtaaaaa tgactgctgg acaaatcaaa gatgccatct
     2221 ccgtcaatca gtcacaaaat tcacaaatgt tctcgatcca agcaacaagc acaaatgcag
     2281 tgacggctca acaaatcgct aatacaacgt cacaagtatt ccaagagaac gccaaagacg
     2341 tgatgaatgt cgataaaatc tcgatcatct caggtgcagt tgccaatacg acacctgttt
     2401 cgccaaataa taaattgaat ctagtgattg gcttagtgtt aggaatgatg gtaggcgtag
     2461 gtctagcttt cttatttgaa ttactcgacc ggacagtaaa agatgaaaaa tatgtgacgg
     2521 atacattggg cttcccgatc ttagggactg taccagaaat gtcagcgaaa gaattgaatg
     2581 ctggtatccg taaaaaagga ccagctccga cacaaccaaa agctacaaac gataaaacag
     2641 cgccatcacg cagaagccgc tcaagagtct aggaggaaag aaaaatggca cgaacacaga
     2701 aacaaaaagc agcccaaact catgcagtca gtttggtcac actcacaaac ccctcttcac
     2761 caatcgctga acaatatcgt acgattcgga cgaatatcca gtttgcctcc gcagctggtc
     2821 agcaaattaa aacgattgtt gtgacttcat caggacctgg agaagggaaa tcgaccactg
     2881 cagcaaacat tgccgttgtt tttgccaaat ctggtcaacg ggtgctgcta gtcgatgcag
     2941 acttacgtaa gcccgtcatc tataaaacgt tcaagttgaa caacgcaagc ggattaagta
     3001 ccgttcttag ttcatcagga agtgtagcag atgagatcca acgaacacca gtcgagaatt
     3061 tgtcgattct gccaagtgga cctaaaccac caaatccatc agaactacta agttcgccaa
     3121 gaatggatca gattttagca gaagcaagac aactatttga tgtagtgatc ttcgacatgc
     3181 cgccagtagt tgccgtaaca gatgcacaga tcatgtcttc caaaacggat ggcaccttat
     3241 tggtcgtacg tgaaaatatg tcacggaaag aatcattgac taaagcaaaa gaattgctgg
     3301 atatggtaca agcgcgagtc cttggtgttg tatacaatgg ggcagaacac agcaaagatg
     3361 ctggctatta ctacgggaat taagaaataa aaagagagga gacaagagaa atgatcgatc
     3421 tacattgcca tatcctgcca ggaatcgatg acggagcaga aaacatagat gccagtatcg
     3481 ccatggctga aaaagcgatt caacaaggga tcacacatat tttatgcaca ccccatcata
     3541 ataatggaaa atacagcaat gaaaaatcac aagtcatttc tctagtcgct tcgttacaag
     3601 cagaattaga gaaaagacaa ttgcctttga cgttattgga aggacaagaa gtacgtatta
     3661 ccggaacact gatcgaagac atcgaccgag atgaaatttt atttacagat ttagatgata
     3721 catatcttct aatcgaattt ccgacactgg aagtgccgtt atatgcagaa cagctctttt
     3781 ttgagttgtt acaactagga aagacaccag tgatcgtcca tccagaacgc aacgcgcatt
     3841 ttcgtaaaga cccgaatcat atgctgccat tcctagatat gggatgcttg gcccaactca
     3901 ccgcaccaag ttatgtcggt gtatttggaa aagacatcca gaaaacagcc aaaatgatgg
     3961 tcaaacataa tcttgtgcag atggctgctt ctgatgccca tggggtctct aagcgcacct
     4021 tttacttaaa agagtgctat aagcagattg ccaaggactt tggtaaggaa aaagtagtac
     4081 agatgaaaca agtcgcaaag gatctgatca acggggatga aatcagttat ccagtctatg
     4141 aagagataaa aaagaagaag tttggtttat tttaggggga gtgtaaggtg tttgtattaa
     4201 caagaagaag aaaaattgcc acattggttg ttgtggacag tttgttattg atcgctgcaa
     4261 atattgcagc agctaaattc atgaagccgt ttgtagctat accaatggac cttattttaa
     4321 tctctaccgg tttgtcgatt ggtttttatt tattttatgg tagtttattc aaagttttca
     4381 ctcggatcaa tcgctataca aatttaagag agatcattgc aatttttggt tcactatctg
     4441 caagtgacgt ctcaagtatc ctcatactgc tgttcattaa tcggcaatac agtttacggt
     4501 tagttatctt tgcttatctg ctttcgctct tattgatcat cggtagccga ttgatctggc
     4561 gtatctacat cgaaacaaaa aatatgagat atgtatcagc ggattctgct aaaaatacat
     4621 tgatcgttgg tgctggcgaa ggcgggcgta ttttatacaa cagctttctg ggatccaaaa
     4681 cggctcaaga tatccaagtt gtcggatttg tagatgatga tccgaataaa agaaatacgt
     4741 atctttcagg aaaaaaagta ttaggtgcat taaaagatat tccggaattg attgaaaaat
     4801 atgacattca aatggtaacg atcgctatcc cttccttatc aagaaaaaaa ttacgaagaa
     4861 tcttcgagtt agtcgaatcc gctcacgtga aagtcaacac gatgccttct atagaagaac
     4921 ttgcttcagg aaaaattagc gtatcaaaat taaaaacaat tgacgtagtg gatttactgg
     4981 gaagagatga ggtaaagctg gatattgaat ccatcaaaga tcagatcaca gacaaagtca
     5041 ttcttgtcac cggcgcagga ggatcgatcg gttcagaaat ctgtcggcaa atcattcaat
     5101 tcaatcctgc aaagctattg cttttaggac atggggagaa ctcgatctac ttgattgacc
     5161 gtgagttacg cacgcatcat caaaattgtc ctacagaaat cgtaccgatc atcgcagata
     5221 tccaagatcg cgagaaaatc aatgagatca tggaacaata tcatccagat atcgtctacc
     5281 atgcagctgc tcataaacat gtaccactga tggaatataa tccaaaagaa gcagtcaaaa
     5341 acaatatttt cggtacaaaa aatgtagcag aagcagctaa agcagcaaaa gtgaaaaact
     5401 tcgtcatggt atcgacagat aaagctaaca atcctccaaa tgtcatgggt tcaacaaaac
     5461 gaatcgctga aatgatcgtg actggattga atgaagaagg ctgtacgaaa ttctcagctg
     5521 tcagatttgg gaatgtcctt ggttctcgcg gtagtgtgat tccagtattc agagaacaaa
     5581 ttgctaaagg tggtccaatc actgtaacag actttcgtat gacccgatac ttcatgacga
     5641 ttcctgaagc tagtcgttta gttattcaat ctggcgcatt agcaaaaggt ggcgaaattt
     5701 ttattctaga tatgagtgaa ccagtgaaga ttgtagacct agccaaaaac atgatccgat
     5761 tgagcgggta tagcgaagac gaaatcgaaa tcattgaaac aggtatccgt ccaggggaaa
     5821 aactatatga agaactactg ttggataagg aaagaaatga tgaagcagtt tatgagaaaa
     5881 tctttgtagg aaacatcaaa ggttactcaa tacaaaaagt gatggatttt gtcaagagcc
     5941 tgccgcaaga cgatgaacaa ttggctaaag atatcgtcac ttttgcaaat gcttcgaata
     6001 aatagggggg aaatataaat gaataaatta gaaaaacgtc agataccatt ttcaccgcca
     6061 gatattactg atttagaaat aaaaaacgtt gtagaagtat taaaatctgg atggataact
     6121 acgggaccaa agacaaaaaa attagaaaaa aaaattacaa agtggttagg aacttctaaa
     6181 gtagtttgtt taaattcaca aactgctgct gcagaaacag ggttacgttt gctgggaata
     6241 ggagaaaatg atgaggttat cgtcccagct tatacttatt ctgccagtgc atcggttgta
     6301 tgccacatag gcgcgaacct agtattaata gactgtcaac aagattcttt ggagatggat
     6361 tacgatgcat tagaagcagc aattacggag aagacaaaag tgattattcc tgttgatctt
     6421 ggtggtatac cttgtgacta cgaccgtatt tttgagattg tggagaaaaa aagaaattta
     6481 tttcaaccta gtaatgagat tcagaaagct tatggtcgta taattgttat ggcagattct
     6541 gctcatgcat ttggagctaa ttggcatgat cagatggttg gtaatgttgc tgacttgaca
     6601 acattttcat ttcatgctgt taagaatttt acaactgcag aaggtggagc tttagtatgg
     6661 aaagagcacg acaaactaga taatgaggca atgtatcatc agtgccagct gctttctttg
     6721 catggacagt caaaagatgc acttactaaa acacagttgg gtgcatggga atatgatatt
     6781 gtgggtccat ggtataaatg taatatgact gatgttactg catcaattgg gttggcacaa
     6841 tttgaacgct atcctaatat tctgaaaaga agaaagaata taattaagag gtatgatgaa
     6901 gcctttcaac aactaggaat tgaagttctt atgcattata cggatgatta tagttcttca
     6961 ggccatttat atattactag agtacctaac attagttctg agcaaagaaa tgaaataatt
     7021 ataaaaatgg cagaacaagg gattgcttgc aacgttcact ataaaccact tccaatgttt
     7081 acagcatata aaaatatggg gtttgatatt aataactatc ctaatgccta tgctcatttt
     7141 gtaaatgaga ttacattacc attgcacacg aaacttacag atgaagaagt agaatacgta
     7201 attaataact atagtaagat tgtaaaggat ttggttggat gataggagag gcaaaatgag
     7261 aaagtgggaa gatttgccaa aagaaatgca gaatgatgaa atacggtatt attatgatat
     7321 cctcagtaaa aaaaaattca gcttagtggt aaaaagagta tttgatattt tagtgtctgc
     7381 aactttgtta ctgacacttt catggttatt ttttattctt gcaatagtga taaaaattga
     7441 ttctcctgga cctgtatttt atagacaaga acgtattact cgttatggaa aaaaatttaa
     7501 aatttataag tttcgtacta tggtaataaa tgcagataaa gatggcactc ttgttactgt
     7561 gaaaaatgat gatcgtatta caagagtagg aaaggtaatc cgtaaatata gattagatga
     7621 aattagtcag ctaattaacg ttttggtcgg agatatgtct tttgtaggaa ccagaccaga
     7681 agttggaaaa tatgtaaagt cgtattcacc attaatgtta gccacattat tattaccagc
     7741 cgggattaca tcagaaacta gtattttata caaagatgaa gataaattgt tgcatgataa
     7801 aaataacatc gacaaaactt atatagaaaa aattcttccc agtaaaatgt attacaattt
     7861 gaaaagtatc agtgaattta gttgtatgaa tgatttgaaa ataatgtgta aaactatttt
     7921 tgttatattt ggtaaagaat atcacgccga cttagaaggt gaacgagagt ttattaatca
     7981 gataaaatga atggaggata agagttttgg tgattgaaga gataaaaagt tcggataaag
     8041 agttaattaa ttcgattgtc gatattcata tgagaacatt tacaaatttt tttttgactt
     8101 tcatgggaaa aaagtttttg gaattgatgt atcgttctta cacagaatat aatgattctg
     8161 gtatattggt tgctattcaa aatgataagc caataggatt tcttgcatat tctggagata
     8221 tgagtggttt atataagtat atgataaaga aaagaataat accgtttgga ttttatagtt
     8281 gtgtagcctt tataaagaaa cccaatatcc ttatgaggct ttttagagct tttctaaaac
     8341 caagcgaaac aaaacgtgat gaaaattatg tggaattagc atccattgga gttaatccga
     8401 aagagaaatc gataggagta ggttccatgc ttatagataa cttaaagagt agaattgatt
     8461 tttcaaaata tgaatatatt gcactagaaa cagatgcatt aaataatgat gttgtgaata
     8521 atttttataa aaagaatgag ttcattttag aacgtaaata ttctactaat gaaggaagaa
     8581 ttatgaatga atatcggttt tatatggaga agaattaatt atgaaattat tgtatatttt
     8641 aaatgtagct aataaagtaa ataacttttc taaagctagt atgatggcag ctaaacaaac
     8701 gggtttagaa ttttatatag ctggtaactg gaaatacaaa tccgatgagg aaagaaaagc
     8761 tgatgaagtg aaatatggaa taaaaatata ccaagttgat ttttatagaa acccactaaa
     8821 tttaaaaaat ataaaagctt atcatcagat tgtagagatt ataaaaaatg aaaaaataga
     8881 tattatccat tgtaatacac ctattggagg tgttgtcgga cggattgctg gtcttaaatg
     8941 taaagtcaaa aaagtaattt accaggcaca tggatttcat ttttattcag gtgctcctaa
     9001 aataaattgg tttatatatt atcctatgga acgttggcta gctcattata cagatgtttt
     9061 gattacaata aatcatgagg attatacttt agctcaaaaa cttcgtctta ggaaaggtgg
     9121 aaaagcttat tatattccag gtgtgggtat agatacaaat aggtttatgt cttccacatc
     9181 aataaggaat tttagagaaa gtatatgtaa agaacttaat atacctgata cagcaaaaat
     9241 cattttatct gtaggagagc taaatgagaa taaaaaccat ttatcagtaa tacatgcatt
     9301 acaaaaaatt ccgctgcata cagatatcta ttatattatc gctggggtag gagaaatgag
     9361 ggagacacta caaaatattg ctaatgaatt aggattacat cagcgagttt ttttactagg
     9421 atatagaagt gatataccca aattattagc tgctgcaaac atatatattc taccctcatt
     9481 aagggaaggc ctcaacgcct ctttaatgga agcgatggct agtggtttac catgtattgt
     9541 cggtaatata agaggaaatg tagatcttat agaagatggt aagggtggat ttctaatcaa
     9601 tcctaaagac gaagaagaaa ttgctggtgc gataaataga ttggctgata acaaattatt
     9661 gtgtgaatct atgggggcac ataatttaga caagataaaa gattttgatt tatcagttgt
     9721 gataaaccaa ataaataatg tttaccatga aattgtaagt aaataattga attgaaagtg
     9781 atattaatat gaaaaaaata aaaattatgc atttgttgca aa
//
LOCUS       DACDCJ010000094         9778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDCJ010000094 DACDCJ010000000
VERSION     DACDCJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.095"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IV038_002462"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IV038_002462"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP8342630.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IV038_002463"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IV038_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP8342631.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IV038_002464"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IV038_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP8342632.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IV038_002465"
     CDS             2857..3732
                     /locus_tag="IV038_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8342633.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IV038_002466"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IV038_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP8342634.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IV038_002467"
     CDS             4629..5063
                     /locus_tag="IV038_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP8342635.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IV038_002468"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IV038_002468"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP8342636.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IV038_002469"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IV038_002469"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP8342637.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT         3427 a         1687 c         2192 g         2472 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaata
//
LOCUS       DACDCJ010000095         9563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDCJ010000095 DACDCJ010000000
VERSION     DACDCJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.096"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(223..1005)
                     /locus_tag="IV038_002470"
     CDS             complement(223..1005)
                     /locus_tag="IV038_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342638.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1070..1906)
                     /locus_tag="IV038_002471"
     CDS             complement(1070..1906)
                     /locus_tag="IV038_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8342639.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1924..3333)
                     /locus_tag="IV038_002472"
     CDS             complement(1924..3333)
                     /locus_tag="IV038_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP8342640.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3347..5170)
                     /locus_tag="IV038_002473"
     CDS             complement(3347..5170)
                     /locus_tag="IV038_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8342641.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6013..6339)
                     /locus_tag="IV038_002474"
     CDS             complement(6013..6339)
                     /locus_tag="IV038_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342642.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6385..6747)
                     /locus_tag="IV038_002475"
     CDS             complement(6385..6747)
                     /locus_tag="IV038_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP8342643.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6784..6999)
                     /locus_tag="IV038_002476"
     CDS             complement(6784..6999)
                     /locus_tag="IV038_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342644.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7126..7461
                     /locus_tag="IV038_002477"
     CDS             7126..7461
                     /locus_tag="IV038_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342645.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7537..9534)
                     /locus_tag="IV038_002478"
     CDS             complement(7537..9534)
                     /locus_tag="IV038_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342646.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2941 a         2007 c         1431 g         3184 t
ORIGIN      
        1 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
       61 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      121 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      181 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      241 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      301 cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca ctgcattact
      361 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      421 atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag gtggtatagc
      481 tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag tatctacttc
      541 tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg taactgcttc
      601 tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt gtccatcttt
      661 agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac cagttctttc
      721 aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga catgtaaaag
      781 cattttgttg tttacatcta cataataatc ctttgactct acttgagacc ttcctatacg
      841 gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa tctgccaatc
      901 tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca cttcactggt
      961 agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat caggagcctt
     1021 agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt cagtacaccc
     1081 ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca cccatctctt
     1141 cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa cgctgcttca
     1201 ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc attcgggcaa
     1261 taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta tcaacctttt
     1321 tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt accgagagca
     1381 tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac gcaatgtttc
     1441 cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg atagcatagc
     1501 agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg cgattttgaa
     1561 aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc aaggtaaaaa
     1621 atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata attcgcttga
     1681 cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc ttttttagag
     1741 catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga accaaatcgc
     1801 ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg ccttgcttat
     1861 ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga ttcaccgttc
     1921 tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc tttctttttt
     1981 tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa aaccataacg
     2041 cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta aataaccaaa
     2101 aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg ctagataatc
     2161 aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg ccccatagcc
     2221 attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca atgttaatcg
     2281 tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag gattttcagc
     2341 tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc tagcggaaac
     2401 atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat taatcgcaat
     2461 aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt taggatataa
     2521 tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa ctgctacatc
     2581 taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat cttctggact
     2641 ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc ctttaagatt
     2701 caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt gattaatttg
     2761 aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg ctccaggatg
     2821 aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt caccatactc
     2881 agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat ttaaccaacc
     2941 gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa tcggttcgat
     3001 tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct tttgattggt
     3061 tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa aagcttttaa
     3121 gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat ccgaggcaac
     3181 ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg gttgtttccc
     3241 atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc ataaaaactt
     3301 ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa ctaatttgca
     3361 tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg taattttcac
     3421 tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg gcagcgaaat
     3481 cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca aatacttcaa
     3541 agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat tcaatacctt
     3601 ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact tctccctcaa
     3661 caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc aatagtccct
     3721 tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt accggatttt
     3781 caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta tcgtcaaatc
     3841 caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta ataattgcgt
     3901 gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag ccaggggaaa
     3961 caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt gcaccaccaa
     4021 tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt gaaggctcag
     4081 taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac tctttgtttt
     4141 tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa gccagcattc
     4201 ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg attggcgtca
     4261 ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc aaaaatggaa
     4321 ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga tcgttcagca
     4381 acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa aataccagca
     4441 tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag ggctctacat
     4501 aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa tctgcagcta
     4561 ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt gaaatatttg
     4621 gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca aactttatgg
     4681 ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca atagtattca
     4741 aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag acggccaaaa
     4801 tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag gtggtagaaa
     4861 tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg ccctttttaa
     4921 ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca ttgccaatca
     4981 ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt ttttctaagg
     5041 ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg gcacaatggg
     5101 ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt cctacttctc
     5161 tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa ccacgcccca
     5221 atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa catgccaata
     5281 acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa cagggaattt
     5341 aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc acatcaacta
     5401 gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac tataccagta
     5461 aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc aaaaatgcat
     5521 ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga atttggtgag
     5581 taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata cttgtttttt
     5641 tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa ggtatcgata
     5701 gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact gatttatttc
     5761 taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata ccaaacatat
     5821 ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa taacataaaa
     5881 attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa tttataggaa
     5941 actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga cggtatgcat
     6001 aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca catatatacg
     6061 gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt ctaatatagc
     6121 tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat cacatgatcg
     6181 atttaagttg tactgttctt gtaaaatctg tgttaccaat ggttttgatg acttctctaa
     6241 tccagatgta cctgtcattt ttgaaaaaat aatacctata atagccaaga caacaatacc
     6301 tataatgaca aacttcttgt tcatatttac tttattcaac tttttatctc ctatctccaa
     6361 aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta caccaaagat
     6421 tgctccagct acccctgcta tcatcaaaat cgtacccatt ttctcatctt tttctcgata
     6481 taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg ctaacgatac
     6541 taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc catgtacgat
     6601 ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgtgt ctgttccggt
     6661 tgtctcatct gtttcttgta gtgctccaca aaactggcaa tacttacttt catctgttat
     6721 ttctttgcca cattttctac aatacatgtc ttctcctcct aaaatcattc aattgcttat
     6781 tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg gtacacttaa
     6841 tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg tttgccacga
     6901 acttacacat tgctgtgtaa cattcatttt ttttgctaac tccgtttgag aaagtccttt
     6961 ttcttttctg tatcggctga gattacttac ttttttcatg attctcccct cctgttttat
     7021 tatatacaac aatatgatgt atttcaactc ataaaatata aaaaacttca aataccatta
     7081 caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa acagaaacaa
     7141 tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa agaattagct
     7201 gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt aacagagcct
     7261 aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag ccattttcta
     7321 gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc tcttagcact
     7381 gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga aaatgaacgc
     7441 aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg tgggtgtatt
     7501 gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa ttgggtcttt
     7561 tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat acagaggaac
     7621 tatcccagct cctgataata tcaattcttt tattgtttca tctatccctg cccctttcgc
     7681 taaatatatt aaaaaactac tacttgcttt cacattgtat agtttcttaa tatccttaga
     7741 agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgtttttta ttaaagcatt
     7801 tttcacgctg ttatccccaa cattacgtag gcgttcacaa acagtgttaa taagttcttt
     7861 ctctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat gtgcttcatc
     7921 taaaatcaat tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa atactctttt
     7981 agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt gatatgtaaa
     8041 atgaattttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca cagaagttgc
     8101 attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc ctaacgcttt
     8161 ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt catcatctaa
     8221 tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt tatctatgtc
     8281 ataagtttta ccatagtaag aactcatttt tttagctaat tgttcccccg acaaaataca
     8341 agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga catcatagac
     8401 acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt tcattgattc
     8461 aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg taggatacaa
     8521 gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct ttaaaattag
     8581 catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac aataaaaaat
     8641 ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt tgtgaaacag
     8701 acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta acaatctttc
     8761 ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa accactgttt
     8821 catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat tgactagttc
     8881 atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa aatcaatcgg
     8941 aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat ctgacttata
     9001 tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg ggatgttctt
     9061 tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt cttttaatag
     9121 aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt gtatcgcaaa
     9181 cgcagaatta tgaactaaat tgatagataa aaccttttct acgcttaagt atctactatc
     9241 atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat cgacatccag
     9301 aataattact ctggtttcca caaaactctc cttgtcattg cctttattct ttgtcttgct
     9361 tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg taacttctct
     9421 atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa ctggcttctc
     9481 taataatttt tcttgatcta cataaattgg aatattaatc attgatgttt ccatttgtgt
     9541 ttcctcctta taaaattctg tta
//
LOCUS       DACDCJ010000096         9419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDCJ010000096 DACDCJ010000000
VERSION     DACDCJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.097"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(150..818)
                     /locus_tag="IV038_002479"
     CDS             complement(150..818)
                     /locus_tag="IV038_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP8342647.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            952..1239
                     /locus_tag="IV038_002480"
     CDS             952..1239
                     /locus_tag="IV038_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342648.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1655..3193
                     /locus_tag="IV038_002481"
     CDS             1655..3193
                     /locus_tag="IV038_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP8342649.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3190..4155
                     /locus_tag="IV038_002482"
     CDS             3190..4155
                     /locus_tag="IV038_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8342650.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4142..5464
                     /locus_tag="IV038_002483"
     CDS             4142..5464
                     /locus_tag="IV038_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP8342651.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5527..5631
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5705..6448
                     /locus_tag="IV038_002484"
     CDS             5705..6448
                     /locus_tag="IV038_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP8342652.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6463..7455
                     /locus_tag="IV038_002485"
     CDS             6463..7455
                     /locus_tag="IV038_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP8342653.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7467..8204
                     /locus_tag="IV038_002486"
     CDS             7467..8204
                     /locus_tag="IV038_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8342654.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8194..8916
                     /locus_tag="IV038_002487"
     CDS             8194..8916
                     /locus_tag="IV038_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP8342655.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9000..>9419)
                     /locus_tag="IV038_002488"
     CDS             complement(9000..>9419)
                     /locus_tag="IV038_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP8342656.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3097 a         1614 c         1978 g         2730 t
ORIGIN      
        1 aataatggca aaataaaaaa acaaactatt ttcgcagatc gtttgcaaaa atagtttatt
       61 tttttataaa gaagatttta ctgaataata tcattttagt ttacataatg atatttctgt
      121 aaattaaata aagtagtgaa aaataagaat cagtgctcta actgttcaag aggatcaagt
      181 ggttcaaatg tttctgtagt ctgttttgct tccaattggg gcaaaacaga tccaatttcc
      241 agatacttca ctttattcaa tgtataagat agaatagaac agctaagtaa tgtatacaga
      301 aaatgtgtga gatccaaata aagcaaggag acaactaaaa taccaaaagt aatcaagaaa
      361 agacttttgc caatcttaat atgaaaatat ttttcaatgg cttgataaat gatatctgtt
      421 ccgcctgtgc ttccgccgaa tcgaaagacg agaccgcaac caatgccaga aagaatacct
      481 gtcatcaaac tattgatcag tagactatct acagcaaaat gtatatgaag gctctcccat
      541 aaatgtaaaa agaaaactag cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt
      601 ccaaaaagaa acaatgagat aagcaataaa ggtaaattaa aaagcagtga agtgacagaa
      661 ggagcgaatc caaaaagatt atgcaaaagc aaagcaatac cagtaacccc accatctgcc
      721 agcttagatg gaatattgat tcctgtcaca gctaatccgt agattcctgc gcctaaaatg
      781 attccgcaca attctgtaaa taaatttaat tttttcattt ctagcctctt ttcttttgaa
      841 aattttcagc aacttcttta ttatactgac ttatactctt acttcaagag gaaaatgaaa
      901 aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa aatgcgagca
      961 gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca aggagttttt
     1021 ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa agcaaatcaa
     1081 tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat ctttcttttt
     1141 ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact cattcctctt
     1201 tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag aaaacaagac
     1261 gtttatgatg tcaaaacaac cattttttac atttttggca aaaaagtgtc atgatacagt
     1321 ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc tactttattg
     1381 gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc tgattccaag
     1441 tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg
     1501 atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat ttttttcttt
     1561 tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg
     1621 tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac aaacattaag
     1681 tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt ctctcatttc
     1741 tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca atcataaaaa
     1801 aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt cacttgcctt
     1861 attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga ttgcacttgt
     1921 tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg aaattgaccc
     1981 gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct tgctgaaagt
     2041 agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct tagttttaat
     2101 catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga catttatttt
     2161 gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca gttctgcctt
     2221 gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac ctcgatattc
     2281 catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat atcagtctgc
     2341 cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac ctgaaatcct
     2401 gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg aattaaaacc
     2461 gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa tcgatatcta
     2521 tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag caatgaaaag
     2581 tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga ataagcaatt
     2641 cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg cagtcaagag
     2701 atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta tctctgatca
     2761 gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata aaggaatcca
     2821 agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt tgcgttacca
     2881 agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata gccagctcaa
     2941 ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt atcaaggcgc
     3001 accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag cactgaataa
     3061 attaagcgga aaaataacag aggatcaaat gattgatatg aattaccaag tcaatgtcga
     3121 aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat tagtaaagga
     3181 ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag
     3241 tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt tttggttggt
     3301 ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc taaaccatca
     3361 gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg
     3421 tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa
     3481 aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc agaccgtgta
     3541 gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg tatgcctaga
     3601 gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc tgcaagccca
     3661 gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag aaactcatta
     3721 caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt cgttacgcac
     3781 aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga tggagaaatt
     3841 atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta cgttcggcat
     3901 ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt cgtcaatgga
     3961 tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga tacgcctatg
     4021 aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga
     4081 agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga ggaggaagga
     4141 aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta
     4201 tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat ggggcgggac
     4261 ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa
     4321 tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca actgggaaga
     4381 acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa aaaaacaatt
     4441 agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg attcatctat
     4501 tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc
     4561 tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact ttttatcatt
     4621 gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt ttgagctagc
     4681 tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc aacggccatt
     4741 aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag catctggcat
     4801 tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc gctatcgttt
     4861 agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg ggagacagcc
     4921 gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac acggaattgc
     4981 agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag atattgtttc
     5041 aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg tagctaagcg
     5101 gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga tcgtctacgc
     5161 cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt cagatcaagt
     5221 ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac cagttgctaa
     5281 ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga ttagcgagca
     5341 agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga ctaaaaaaga
     5401 gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct atttattgaa
     5461 ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta cactttaaaa
     5521 aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt gaacttagac
     5581 ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc ttgaagcggg
     5641 ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga aaggaggtaa
     5701 caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa ttatttggca
     5761 gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg ctgctgtatt
     5821 ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga cattaaatga
     5881 agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct tggcaatgga
     5941 ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac aaatgttgcc
     6001 aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc ttccaaaagt
     6061 gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg atacattgag
     6121 gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata cagggaagat
     6181 ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt taaaagtggc
     6241 tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt
     6301 agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt ttgctgcgat
     6361 catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat tgaaaaaagg
     6421 gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa attatggctt
     6481 ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac aagtagcaaa
     6541 caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc gaatactaat
     6601 catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg tttaaatgtc
     6661 caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc ggacaaagca
     6721 caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg acagaatata
     6781 ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc caccctatca
     6841 gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt
     6901 ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga cttttcaaaa
     6961 ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc ctcagatatc
     7021 caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat tgaactaaac
     7081 tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt aattattacg
     7141 aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga tgccacgaaa
     7201 aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt gcaaaaatat
     7261 gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc aaaaaattat
     7321 acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa ttatacagct
     7381 tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact attcactaat
     7441 cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg aatgtagtca
     7501 ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat acctttacat
     7561 cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta acaggagtcc
     7621 agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt ctccatacag
     7681 catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc tatcaaaacg
     7741 ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta agggattatc
     7801 tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta tctggcggga
     7861 tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg
     7921 atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac tggatgttga
     7981 agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc gaagaagcaa
     8041 tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga
     8101 ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca gagttgaagc
     8161 aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca ttgtcattta
     8221 gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac gatcatcaat
     8281 tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa gactcaagcc
     8341 ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca agttgagcct
     8401 tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg gacgaatgta
     8461 ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc cataaatgag
     8521 aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta tatccaagct
     8581 taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca agactatatt
     8641 gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc agtctggcaa
     8701 ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact acatgcagca
     8761 gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct tgaagaacag
     8821 ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa aaatacttat
     8881 caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac acaactagtg
     8941 tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat aaatgaatct
     9001 tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa
     9061 gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc atttaagtaa
     9121 gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt
     9181 gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt agcagcagtt
     9241 tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga
     9301 accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac ggctgcttga
     9361 ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DACDCJ010000097         9337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDCJ010000097 DACDCJ010000000
VERSION     DACDCJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.098"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IV038_002489"
     CDS             complement(<1..281)
                     /locus_tag="IV038_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8342657.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLY"
     gene            complement(429..987)
                     /locus_tag="IV038_002490"
                     /pseudo
     CDS             complement(429..987)
                     /locus_tag="IV038_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302360.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1156..3267
                     /locus_tag="IV038_002491"
     CDS             1156..3267
                     /locus_tag="IV038_002491"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF019826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8342658.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            3245..3895
                     /locus_tag="IV038_002492"
     CDS             3245..3895
                     /locus_tag="IV038_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8342659.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3882..4334
                     /locus_tag="IV038_002493"
     CDS             3882..4334
                     /locus_tag="IV038_002493"
                     /inference="COORDINATES: protein motif:HMM:NF012579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8342660.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4351..4656
                     /locus_tag="IV038_002494"
     CDS             4351..4656
                     /locus_tag="IV038_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HAP8342661.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4668..5945
                     /locus_tag="IV038_002495"
     CDS             4668..5945
                     /locus_tag="IV038_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IIABC"
                     /protein_id="HAP8342662.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5964..7112
                     /gene="dgoD"
                     /locus_tag="IV038_002496"
     CDS             5964..7112
                     /gene="dgoD"
                     /locus_tag="IV038_002496"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP8342663.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            7124..8026
                     /locus_tag="IV038_002497"
     CDS             7124..8026
                     /locus_tag="IV038_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryldialkylphosphatase"
                     /protein_id="HAP8342664.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8695..9081)
                     /locus_tag="IV038_002498"
                     /pseudo
     CDS             complement(8695..9081)
                     /locus_tag="IV038_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9141..>9337)
                     /locus_tag="IV038_002499"
     CDS             complement(9141..>9337)
                     /locus_tag="IV038_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8342665.1"
                     /translation="NNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRN
                     GTLFGFSVSTEIKVLMGITA"
BASE COUNT         3012 a         1447 c         1904 g         2974 t
ORIGIN      
        1 ttgtacaggt gtagataagt tggtacttct tgatacggaa gcaactgaat ccaactatca
       61 tgataagcat gacggatgac agaatcaaac ccacaacatt cgcaacgatg cgcttctcca
      121 gttaataagc cggtaaatac attggccata cggccatgaa cgcgctcttt actaatggca
      181 tcttttgaaa aggtaatatg ggaatctttg atgttcaaaa tttgtttgat ggaatgactt
      241 gtcttttcat ccgtacattt gatattctta gttatggaca tttggttatc ctcctatgat
      301 tgtttttgcc gatttaatta taggacaacc agatgtcttt ttgttataaa aaaatatttg
      361 gcgtcactac tcagtttgag taacaaaacc aaatattata gaaccggctt tttcagtttt
      421 ttcccttcac aaacaacatc gatgattttc tcggcatggg tacgggtatc attcattaat
      481 tctgtatcgg ttggatattt gatgttttgt ggagcacaag tggcatccaa aatcaatgtt
      541 ccggaattga aatcgtcata agaatcgttg tccttatcgt cttttttctt gtcgaagacg
      601 agtatttttt cattgatttc tatcaacgtt gtttcgtcca agcgtttccg gaaatagacc
      661 attgtagagc atcgaaaggc ttggcatctt gatagcccgg caaaccgatg aagaactata
      721 aatacgggtt ttcttgaatt tgtaagaccg tttcttcgtc gctatagcta tattcctttt
      781 gaatcagtag agctccgaag gtcatgcgaa agggttttgc gacgtttcct tttttttacg
      841 aaaattttta gcgtagtctt tttcaagttc agcccatgga atgagctctg ctttttgaat
      901 ccaacggtta gacggattca ttttgagacc tatcggttga ttgaaatctt caaaagccaa
      961 ttgaacggtt gtttgtaatt tatacatgaa aagcctccaa aattgagtgg tactccacac
     1021 cattctgatg gtgcataaga taaaaaatta aagaagtgcc tgtcatttgt ctgatttaag
     1081 ccaaggtttg acaaattttg gatattccaa ttaatactgt aagcggttat atttaactca
     1141 gggaggtaga tgaatgtgaa tacaaaagat ttaaaactgt tagaaaccat tgtacgatca
     1201 catgtgacaa actctcaaca attgaaatca ttagtacatt tttcgactag gcagataact
     1261 tactctataa aaaaaattaa tgatgaatta tcccttcatg gattgagtct gataaagcga
     1321 agtaactcag gggagtggtt atatgaagcc aatgatcttc aaaaactttt gatatacatg
     1381 aatcaagcaa tggtttttaa agaaggtgcg attcctgaat atataaacga tgatttggct
     1441 tatagtgaga taagaagata ttcggaactg ttatttattc tagccaaccc caaaaaagtg
     1501 acaacccaag aaataatggc gtttattggt atcagtaaaa atacggctag aaacgacttt
     1561 tctaaaataa aaaaagagtt gccaccagac tacactttga tatttgatcc ccaaaatggc
     1621 tatatgattg aatcaagcat ggcaaagctt catacattgc tcttagaagt tttcaattat
     1681 ttatttgaaa ggcatgcaac agttaaccgt tttgagtcac tcttttctga agaaagggaa
     1741 tttgttggtt tttttattca cacctacgaa caaactttta aattgaaatt ttctgataat
     1801 tcactaacca aactttatta tttgataggt ttatcacttt ctagttttaa aagaaggacg
     1861 aaaggacaga ttgattttag ttatgattta caggaattag atgagtttca aatgattcga
     1921 agctgcattg atcaacatta tcaacatgct gagatagcta tagattatga atggttgacg
     1981 cttctgtttt tatcattcaa tacaatttca aatgagtttg aaattttaga ttcaagaatc
     2041 tatcaagtaa ttatggagat gattacctta tttcaaagta aatcagctat tcggattagt
     2101 aataaagaag agttgaccaa tcgattgatg aatcatttac gacctgcaat ctatcgaatt
     2161 cgctacgaat taccatttga aatagatgat ttagctgaaa ttgttaagga aaaccatgaa
     2221 gttagtgcgg tacttaattt tgttaaagaa tccattgtac ctttggaagc atttttgcgt
     2281 aagacgattc ctgaaactga attatatttg atttctgttt atttttgttc ggagctatta
     2341 aattcttcta ttatcgaaac acctgttaga aaaaaggcaa tcgtcgtatg tagcaatgga
     2401 ttgattatgg ccaatgtaat ggcgaaaaca ttgaaagaaa tttttccaga tattcagttt
     2461 gttgctagtt cttcggtcag agagatggat aagtttgtcg aagatgttga tttgattttt
     2521 agcaatcaac cattgaaaag tgaaattcca ttatatatta tacaaccgat tatgaacgaa
     2581 gaagaaaaaa accgcttgcg gaaccgtgta ctcattgatt tttctacgaa taatatcagc
     2641 aaagagacta ggaaccttac tgaaattatt aagcgttact ctgctgttca aaatgaagaa
     2701 agtctacaaa ctgatattga gagattttta attaattatg caaattcact aatatatcta
     2761 gatcgtcaaa gtaaacaggc acaaaagata tcttttgtgg aattgcttgt ttctgaaggg
     2821 attattcttg tatctacgaa acaacttact tggcagaacg cgatggaatt gtcttgtcag
     2881 cctttattaa aggagcaggt aattaatcag aactatcaga ctattttaat gagtcaaatg
     2941 gcggatgaag aggcttatta ttttcttaat aatcgtattg ctattccaca ttcatcgcca
     3001 gaaaatgggg taattaaggg cggttgctcg ttacttatat cagttgatcc aattattttt
     3061 cctggaaata ggcctatcca ttttatttcc ccttttgcaa ttggaaagaa ggaaaattat
     3121 ttacttgcga ttaaccaatt actacaactt tctaaggacg aggaatatca gcaagaaata
     3181 cttctttgca cgacggaaga tgaagtgaga aaaaaaataa aaaatttttt aaggaggaag
     3241 caatatgtca ataacgaatt ttcctgaatt aaccatcatt ttaagaggat atagttttga
     3301 agaggcgatg tttgtcatgg aagaattggc tgcgatgaac acttcggtag gaattgaagt
     3361 aacaaccaat aatgatgact ttgcacgtat catcacagaa ggcaacaaaa aatttgggca
     3421 acaattattg attggtgcag ggacagtttt aacattttca caagcagatc aagcaattgc
     3481 tggtggtgcg aaatttttat taggaccaaa aaaattttca acaccaattt tttcgttggc
     3541 taaagaacgt ggggtggtaa ctgtgccatc agcttttacg ccatcggaaa ttcaagttat
     3601 gtttgatgaa ggtgccgata ttgtaaaggt ttttccagca gtcactgtag ggccaaccta
     3661 ctttaaacaa ctggccggtc cttttggtat acggccttta atggcagttg gaggagtgac
     3721 gattgataat attgcggctt ttaaaaaagc aggagttgtg tactttgggg taggatcaag
     3781 catgtttcaa aaagctgata tacaaaaccg caatgggcaa gctattcgaa aatcaattca
     3841 atcatattta gataaactag ctgaagcagg aagtgaagaa gatgagtctc attaaattag
     3901 aagaaaaaaa tattttgtta aatcaaagtt tttcaaacca agaagaatac tttcaaaaga
     3961 ttacaaagaa actgatgacg gaaaacatgg taaaggaggg atatctcgag tcaatcacta
     4021 agagagaaac aacttatcca acggggctat atattgggga acgctcaatc gcaattccac
     4081 atacagatta tcagttatca aatgttaccg ctattttagt atccgttctt aaagatagtt
     4141 tgcattttca gcgaatggat gatcccgaag aaatggtttc agtgaatgtt attattcacc
     4201 ttttattcga tagtcccgat aagcaaccgc aattattgaa ggagttgatg agtaaagtta
     4261 ccaatcagga tttcttacaa gcaattatgg aagcaaagag taaaacagaa gttttaactg
     4321 catttaatca ataaattgga gggaaataca atgagtaaaa aaacaattat cgtcgcatgt
     4381 gcatcatcaa tgattacatc tacaatggca gctaataaaa ttcggaaaat agcaaaaaca
     4441 aatggattac cagaacctcg aattttgcaa ctaaaatttt cagaagttgc aggtgcttta
     4501 gaatcaaatc aagttgactt tatcgttcct acagggaaat tacaagaaaa tgtaaccaaa
     4561 ggaattaaag ttatacctgg aacaccattt gttactggag tgaatgaaga aaaatctgaa
     4621 tctttggtgt tagaagcatt gaaagagctt gactaggagg gtgaaaaatg aatattgtca
     4681 ttgatgcgtt aaactggatc tcaagtttgg gtccgatgat tatgatgcct atcattatct
     4741 tgattttagg attatttttt agaattaagc taaatacatt actaaaatca gcgttgactg
     4801 tggggattgg ttttgcgggt gtaaatatta ttattaattg gtttatttca caggtaggtc
     4861 catctgttca gttaatggtc aaaaattggg gtattcaaac aagtattatg gatgtcggct
     4921 ggccagctcg tgcagcagcc acttggtctt ttcctctagc agcagttatg gtatttatta
     4981 ttttgggtat taatgtgttg atgctgattt ttaaaaagac caatacagta atggtcgatt
     5041 tctggagtta taatcactat atttttgtag ctgctttggt ttattatgca acggataaaa
     5101 atattatatt ggcggtaatc ttcggggcga ttgatgcaat tattagtttt aaactcgctg
     5161 attggacaac gccgttagta gaggattact ttgaactaga aggggttaat ttcccgactg
     5221 cgaactcagt tggttgggca cccttggctt ggctacttaa caaattgtgg gaccgcattc
     5281 ctggattaaa taaggtagat gctcgtcctg aaaaaattca agagcggttt ggttttgttg
     5341 gtgagcctat gtttatggga tttgtcatcg gcttggttat tgggttatta gcaaaagaac
     5401 ctgttgataa ggtattgata gtgggaatga gtacttctgc aacaatggtt ctaacaccta
     5461 aaatgatgca aattttaatg gaaggattat taccctttgc taattctgtt aagaaattct
     5521 tgaatgtaaa atttccagga aacaatttta caatgggtgt ggatgctgcg ttgacagttg
     5581 ctaattcaag tgccattgct acgggtatta tcatggttcc gattacctta gtgttggctg
     5641 ctattttacc aggtaacaaa ctcttaccta tttcagatat tgcctaccaa gcaatgtggc
     5701 tatctgcatg gccggtcgcc ttttcaaaag gaaatattat tcgcggaata ctttccacca
     5761 cgattataac agcttgtgtg cttttaattg ctactagttt agccggcata catacacaat
     5821 tagctttggc tgggggattc caattaccag caggaatgac acaaatatct accgaagatg
     5881 caggtacaca cattattggt ttcattttaa tgaagattgc tgagtttatt tcaaatattt
     5941 tctaatattc aggagggcat acagtgaaaa tttcaaaaat tacagtctat aaagtaaaac
     6001 caagatggat ttttgtcaaa atatctacag atgaaggtat tgaaggctgg ggtgaaatga
     6061 tttctggaac taagactgaa acagttgtgg caggagctta tgagatagga aatcgattct
     6121 tagttggtca aaacccattt gcaattgaac gtctgtttca ggaaatgcat cgttcatttt
     6181 tccgaggtgg tcctattaat ggaacgattg tctctggact tgaaatggct ttatgggata
     6241 ttaaagggaa agccttaaat gttccagttt atgaattact aggtggagca gcccgtgata
     6301 aaatcaaagt atattcttgg attggtggag atcgtccttc tgaagtagta gagcaggcac
     6361 aagatcgagt agatcgtggg tttaccgccg tgaaaatgaa cgcgacagaa gaattacact
     6421 acattgattc ttatgaaaaa gtagctgaag tagtggaacg tgttgcttca attagagaac
     6481 gttttggaga tacattgagc attggtgtcg attttcatgg tcgtgttcac aaaccaatgg
     6541 ctaaggtttt agcaaaagca ctggaaccct atcatccaat gttcttagaa gaagtggttt
     6601 tgccggagaa tgaagaacat ttcagtgaag tagccaatgc agttgctgta ccactagcaa
     6661 cgggtgaacg tttgtataca agatggcaat ttaaatcaat tcttcaacag ggagcgattg
     6721 atatcattca accagatgta gctttatgtg gagggatttt agaaacaaga aaaattgctg
     6781 caatggctga agcttatgat atagcagttg ccccccatgc accatatgga ccgattgcgt
     6841 tagcagctac attacaagtt gattcttgta cacctaatgt gtttattcaa gaacaaagct
     6901 taggtattca ttacaacaaa gggtttgatt tgttagattt tgtaaagaat aaagagatat
     6961 tccaatacaa agatggcttc gtagatcttc cgacaaaacc aggtttaggg ttagaaatgg
     7021 acgaagatcg agtgaaagaa attgcacaag aaggtttagt ttggaccaat cctaagtgga
     7081 aaaattatga tggtacaatc gctgaatggt aggagatgag tgcatgatca atacggttac
     7141 aggcccaatc gaagctgagt ctttgggtac aacatttatt catgaacatt tatatgtcat
     7201 tcctaatgag ttaagaaagt atgaagatta taccttggat gatatttatc atagtattgc
     7261 ggaagttatg gcttttaaac aagctggtgg aaaaactatc gttgatttga caccaattaa
     7321 ttatggacga aatccgttag ctttgaaaat gatatctgaa gctaccggag tgaatgtagc
     7381 atttgttacg ggattccata aggaagagtt tcagccaaaa tggttgacaa cgctcaatga
     7441 tgatgagatc tttgagttgc tttattccga aataactcac ggagtcactt cacaaaacct
     7501 tttgccagga gcaatgaaag taggtacgag taaaaatgag gttacattgg ctgaacaacg
     7561 aatactagcc atagccggtc aagtacaaaa aaaaacaggg attcccatta ttacgcattg
     7621 tgatgggggg acgatgggac tggagcaaat taattgttta acaaaaaacg gtgcgaaagc
     7681 aagtaatatt tgtttgagtc atgtagattt aactaaagat tttcactatt tagctcaaat
     7741 ctgtgaaacg ggagcaagta tttgtatgga tcatattggc cgagagttgg cgaatcatga
     7801 tgcagaccgc gtccaactaa ttaaacgact ggttgatgcg ggttatgaaa atcagatttg
     7861 tctttcaggt gatatgggac gaaaaaaata tttgccaact tatggaggaa cgcctgggct
     7921 tacctatatt ttaacaagtt tgaaagaaga actattaaaa gtaatgagtg aaccaacttt
     7981 taataaaatg attgagatga atccaattaa tatactgagt aagtgaagtc aattgaatca
     8041 ttcagaagaa ctaatgaaat tagttgcttt cgcagggtta attcttctct ctgggtttgg
     8101 aaaagagggc ttagcttcgg attccctagt taaactaggg gttttatcag gttattgtac
     8161 tgattataag tacctgaatt attcatgaaa tactttttcc gtcgttttat aggcagttgg
     8221 gtcaataaaa agatcagaaa aacgaacgag agactctcat aacgtgccac taaataaaaa
     8281 tcgtacgtta ttttaaaaaa tgggttgatt ctaggcagta ctttgtcggt ctatttttag
     8341 aaaaagaggt tctttgtcaa acatacatga attcgcaagc tcattcaccc tgaaggatga
     8401 aaggcagaaa ctgtagtata ctaatggtgg aaaggaaaaa aagccgccag caatttggaa
     8461 aagaacgtta tgtcgattgt caaactggct tgctttattt ctcgagaacc ctgaaatata
     8521 gccaatgagc atgtgtataa aaaattgatt ttatatgaaa taaagcactc ctttcctaaa
     8581 attattggaa aggagtgcgt tttttatgga aattatttag gttctttgtc aattgctatt
     8641 gttttgctca aatcgtatgt taggaaatgc ttcggcattt ccttttttgc gttattaaat
     8701 gatttcacag atgttctgtt gaatcaggac acgcatccgg agatgacgaa aagtccgaaa
     8761 tccaaaagca attcttttga ttactttgat gaggttattc tttccctcca attttccgtt
     8821 tgaataggga tattttaagg agtttgtgat tgcttctgtg tgctttttca gataacaaat
     8881 tgccttttta aacccatcat tcaaggaatg tggcaatgat tcaatgagat tgaaaaattc
     8941 agtaaaatct tttgcatcaa aagcagctaa taactcttga tagacttcat agctttgtcg
     9001 tagttggtta tctatagaga gtaggtaatc aagcacctcc gactccgtca tgtatttccc
     9061 ttggaagagg ggaaattgct taaaagggtt ctgttgcgaa gtttgaaaat caaacgcgcc
     9121 ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat ttcagtagac
     9181 accgaaaagc cgaagagcgt tccatttctt cggttctttt tatatattcc tcgaatggtc
     9241 tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt atttcgtcgt
     9301 ttaataggtc gatggtcttg ttctattaaa ttgttaa
//
LOCUS       DACDCJ010000098         9272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDCJ010000098 DACDCJ010000000
VERSION     DACDCJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.099"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2180)
                     /locus_tag="IV038_002500"
     CDS             complement(<1..2180)
                     /locus_tag="IV038_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP8342666.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEK
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKYENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     EYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDIL"
     gene            2663..2878
                     /locus_tag="IV038_002501"
     CDS             2663..2878
                     /locus_tag="IV038_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP8342667.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            complement(3119..3305)
                     /locus_tag="IV038_002502"
                     /pseudo
     CDS             complement(3119..3305)
                     /locus_tag="IV038_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            3739..4698
                     /locus_tag="IV038_002503"
                     /pseudo
     CDS             3739..4698
                     /locus_tag="IV038_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            5229..5639
                     /locus_tag="IV038_002504"
     CDS             5229..5639
                     /locus_tag="IV038_002504"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP8342668.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            5632..6087
                     /locus_tag="IV038_002505"
     CDS             5632..6087
                     /locus_tag="IV038_002505"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8342669.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            6104..6835
                     /locus_tag="IV038_002506"
     CDS             6104..6835
                     /locus_tag="IV038_002506"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8342670.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            6852..7664
                     /locus_tag="IV038_002507"
     CDS             6852..7664
                     /locus_tag="IV038_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8342671.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            7812..8999
                     /locus_tag="IV038_002508"
     CDS             7812..8999
                     /locus_tag="IV038_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP8342672.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         2976 a         1706 c         1638 g         2952 t
ORIGIN      
        1 tctaaaatat catatacatt gatattagga attgaaaagg aacgtggaag atccgaagtc
       61 gtgatgacca attttgtctt gtcaaaataa gtttcatcat tttggtcgat taattcacgt
      121 agttctctgt attccttcgt gaaaacttcg atggactcat ccgataaatg atgacgaaaa
      181 acatccttca gtttatccga gatacccaat cccgacatgc aggaaatgat gatgttgcta
      241 ctttgagaaa agccttcaaa gtattgaaca ttgattgaat acttgtctgc tgcatcctcc
      301 gcaatctgtt taaatggcgc attcatcgtc atcttcaggc caatatcaag tgcaatggag
      361 gtcgtcaaat tattcagaag aagtaattct ccttttaaat cgttcttgat ctgtgtgtag
      421 atttgattta aagagcccat atctacgatc agcactagac tatctgtatt cacttgccgt
      481 ttcacgaaat tccttgtcga ttcgacaatc tctgacacat tactctctac tggcatatcc
      541 aacgcttcaa aaacataatt gccgcataat tgattcacta ctgcttgaat gctgctagcc
      601 gtactatctc cgtgagcaag caacaggccc ttcagctcga tggattcatc tacataatca
      661 gataaagtca cgacaataag cagtcttaga aagttctcgt acttttctga gagcggtggt
      721 aaatgctcca agaactgatt acataagtaa gtcgtccgag gtagatttgc agctaatgta
      781 cgatttatcc cggttaagtc tgtcttatag cttggggaaa tgacatagga tctgctaata
      841 atttctgcaa caggtccact cgtcgtcaat ccgtacgttt ttcctaaaac ggatacccag
      901 acctttctaa acatctgaat catcaatttt ctactcattg ataaaaaaga atccttatca
      961 atgtgatgca ccaaatgctg tacttcattc tttatcgaag aaaacttttc attgaaatta
     1021 ccttcattta tcagctgagt aattttttcc aaaatcgaat cataatcaaa cggtttgatt
     1081 cgatccgttt ttatcttttg atttttcttt atcaataaac tagcaatcgg caagctttct
     1141 acttcggatt ccagcatatt taactgaatc aagatctcat ttttcccaat cgttaatggc
     1201 tcttcccgtg ttcgactgta cacatccgca caactgattt taatgatatt cttcagctta
     1261 ccaatattcc cactaaaggg atgagataac attagagaca aagcttcttt tgaaacaata
     1321 atatcttttt gtaaaatatc cgcttcattt tgaaaaaata attgaatcag ctctaaccgt
     1381 tctcgaatgg gacgatcttc aaaagttggc aacataacac tgatttgaat acgtcgatca
     1441 aaggtgtcta ataaataatc ttcacttttt tccgtcgtgg cgaagataaa acgaacctta
     1501 gcagaatgcc agtttacatt ttcaccgact ggacgataat ttccagtatc aataaaaaca
     1561 aagagctttt cttgattttc tttcgacaaa cgatggacct catcaagaaa aagatagcca
     1621 ttgttggctg ttgccaataa cccctctgta tccttctcag cccccgtaaa ggcccctttc
     1681 ttgtatccaa acagtgttgc cgaaagcagt tcgggattgt ccgcataatc ggcacaattt
     1741 aaaatagcaa atggtgcatc ttcttcaata atttgctcgt ggatagcgta ttcaaaaatt
     1801 tttgtggcta aaaaactttt tccaaccccc gtggctccat ttattaatac actgagtccg
     1861 ttaggcggat atttgacagc tgcaatacat ttttgaatga cctctctcag acttccgtca
     1921 tgacccacca cagaatgaaa ggcttcgttt atttgctccg tattatctac aaccgcatat
     1981 tcatctaaag gcttccacaa aacaggcttt cccggtaatt tctcgacctt tttatcttcc
     2041 actaatctcg tcaaataatg gctggcattc tgacgagaga tacctagatt cttcgaaatt
     2101 ttctcggcag tgacagaaga catattccct gttagtcgta tcagctcatc aaaaactcga
     2161 tctttagcgc tctttttcat tttcccctcc agttcaacgt gtctagaagc ataataaact
     2221 atcggctaca atatgtctat tctatatttc tgttccaatt ttactttgaa acaaaaataa
     2281 tcgagcttaa actgatatta gttataaaac tatagttaga ttaccaatct tcctctcata
     2341 caataaactt ctctcacctg catccaacct acttatgtga aagaataact gccgattatt
     2401 tatctttttt acagttactt tcgaacataa aaaaatcacc ttcaaatact agaaaaacta
     2461 ctatttgatg gtggtttaga acaatttccg ttgattcagt atcgtctggt cccacatcct
     2521 ctgtctcatt aatcacaata gcatactcca aaggataaag ctggttgata tctcaatgcc
     2581 tttcacacct atcaattttg tcacacttat cttttcctag gttctattct atataatcta
     2641 gaaaaacaaa gaaggagaca ccatgaaaaa attcattatt ccttgtatat ttcttttctt
     2701 gagtattttg ttattttcaa cttccatcct agtcggttct tacctcgatt caagcggaat
     2761 gctgattgaa ccagcgttct tttgtgttcc gttgggctac ttaacattag gacttgcttt
     2821 ctttactgcc ttatacagtt tcgtcaaaca aagattattg aagaaatact cggcttgatt
     2881 tgcacgtaaa tcctctgttg aagaaaagtt agttttttca acaacgaacc ttcgagtatt
     2941 aagaatggat gttgatgtag acatcaaacg tctgcataaa cattcatttt attcctccat
     3001 taccaacaat tattcccaat cgccaaaaga agcttttttc ggaattacct tctccatttt
     3061 ttcacctgct ggttatattg tcttttgtaa taccgatagt cctcttcaat cattttcaat
     3121 atgcgaaaag aaccaatttt aacatggcac tcaaatcaca attcctaggg cggccaaaaa
     3181 gatacggctc gttgatttca agggtttcaa cgagttcatt gacaaaccca gcggtgctat
     3241 cttcttttgg aataagaagt tgtgatatct aaagtgagtt gattcatgtt ataatttgta
     3301 tgcatgattt tgatcctttc aagggttgaa tgatttagag tgcgagtatc ctctcgtact
     3361 cttttatttt acctaaaaag ctccagaaat gacgaatgac tcgtcatttc tagagctttt
     3421 ttcagagaat tatgtcacaa tctctttttg ttatattgat attctatatc tttttttatt
     3481 ggttttattc ccttgttcgt tatagactaa aatccttctt tcagcattat tcaaatcaaa
     3541 tggcaacata atagagaaat ttatagtatg tataatcatc tgaattcaat caaatcaacc
     3601 ggagtcatat acggagtcaa actatcccac tgagagttta ttaaacgcag attgtaaaat
     3661 taagctagac aactaaaaaa gtcatttgtg ctacactcaa aatagttccg accaaagaat
     3721 tataaggagt gaagacaaat gacctatacc catcttacaa caaatgaact cgtgatgata
     3781 gaagcctatt tcaatatgca tcaatctgtt accgtagtcg ctaagacctc aaatcgttcc
     3841 agacagacca tctacaatgt gtacaacttc ctgaaacaag gccaatctat ccatgactat
     3901 taccaacaat atcaaaaaaa taaaacacgg tgcggccgac gacccatttg tttgccagca
     3961 gaacaagatg cgtatatccg aaacaaggtg gcgcaaggat ggacacccga tgtgattatc
     4021 ggtcgtgctg agttttcaat ttcatgttct gttcgaaccc tttatcgcaa gtttaaaaca
     4081 aactaatttg attgctcgaa actacccatg aaaggaaaac ggaaacccaa cgggcataaa
     4141 gaacgtcgag ggaaacaagc cttcaaacgg aatatttcag aacgtctcac ggactatcct
     4201 gaatttcaac aggaatttgg acatttggaa ggcgacacta ttgtgggtgt ccatcataaa
     4261 agtgctgtca tcacgctggt tgagcgcttg tcaaaagtga ttattacttt aaaacccgaa
     4321 ggacggaaag cactagatat tgaaaaagtg acaactgact ggctacaaaa gcttccccga
     4381 aacttgttca aatccattac ctttgattgc ggaaaagaat tttcgaattg gaaaaacatg
     4441 agtaatgcta acgatatttc tatttatttt gcagatcctg gaacgctgtc acaacgggga
     4501 ttaaatgaac attctaatgg attgttacgt aaagacggac ttcctaatac aatggatttc
     4561 aatcaggttg accaaaaatt tgtggcggcg gttgcctcca aaagaaatca tataccaagg
     4621 aaatctttaa attatcgaac atcgttagaa gtatttttga gttacataag tgaggagcaa
     4681 ttgtctaact taatttgaca tttagaaaat aataaaccat atcccatcta ttcaaataat
     4741 attttaccga ttgaaagact tgctaatcaa agcagaatag tttatataga taaattattt
     4801 tctttaaact tatactcatt tttagatatt ttataagaat acttcatgtc ttcgccagta
     4861 atataaagaa catctgtctc tccatttgga cttgggttat ctattttggg tgtaacatac
     4921 tatacataca accgatacat aaaagatttg acatctggac aaaacacaat gctttacttc
     4981 atttttatag gctttgttta caccatcaca aaaatcacct cctaatttac aaattcatta
     5041 aacaaacaga aataaaaaat caccacgata gccgcgttca aaaaaattta caaaagagaa
     5101 ttttagttaa agtttttaga ttcgccccac taattaccaa aggtatttta tttattctta
     5161 atacgaaata atttattgac atttagatta ccaatggtat attatttata aataaatgga
     5221 ggtggattat gtacacaatt attcttgcaa gccacaaaaa tttagcgaaa ggaatgaaag
     5281 agacagtcga atatatcctt ggccctgcac cattcataaa agtaatgact gcatacacag
     5341 acgataacta tgatattgaa aaagatacga atattcttct cgatggaact gataaagtcc
     5401 ttgtcatcac tgatttactt ggtggtagcg taaataatca ttggatgaat tatgtttacg
     5461 aaaaaaaatt aacaaaaaaa attacagtaa ttgcaggtat gactttgtcc ttaattatgg
     5521 aattaagtat gaacattgaa gattataaat tgagagaaaa aatttccatg attattgctg
     5581 aaagccaaaa gagcattata aactgttcag aattaatgga ggtagaagat aatgattaaa
     5641 ctattaagaa ttgacgatcg cctgatccac ggtcaagttg caatggcttg gactaagtcc
     5701 gtatctgctg actacattat tgtagttgat gacgcctctg ctaatgacaa aatgaaacaa
     5761 atgattttat cattagcaaa acctgtatca acgggactag atattattac ttcagcagat
     5821 tttttaagta cctttgaaaa aaggaaaaat caaaatttga tgatagtcac tagttcccct
     5881 aaaagcgcat ttgaaattgt aaacgctatc ccaggggaag tcgattcaat taatatcggt
     5941 ggtttacggt acgccgaggg aaaagagaaa gttaacgagt atgtggctat cacatcagaa
     6001 gacaaaaact acttagcaaa attaaaggag tccggcttca aattaattat gcaggctact
     6061 ccaactggaa aagcgaagat ttattagaag gtggggaata agaatgattg aatgcatttt
     6121 aattggcata gttgggtatt taacgacaat agatgaaaga tattttgggg catctatgat
     6181 gaatcgaccg ataatagttg gacctgttgt tggacttatc ttaggggatc tccatcaagg
     6241 catcttgata ggagcagcat tagaagcaat gtttattggc attgtaacga ttggtgctgc
     6301 actaccacca gatgtaggtg tagctggtac tattgcaacg gctctagcaa taaaaagtgg
     6361 agcaggtgct gatgtagctg ttacattggc gatgcctttc gctgtattgg cacaaggatt
     6421 aaacatgttg gcttttacga ttaactcctt taccgtttca caaggtagaa aagcaattga
     6481 aaaccgcaaa ttatcatctt ttcagaagtg gcattttttt ccattaatcg ttcgcttacc
     6541 ttccgcagta cttacatttt tagtagtata ttttggtgcc aatgctacag aaagttttgt
     6601 aaatattatg ccagaaaaat taatgaccgg tttcaccgta gcttctggtt tactccccgc
     6661 tgttggattt gccatgctaa ttcaaggaat gattgacaaa aaatcagttc cttatttttt
     6721 cattggatac cttttagcgg catatttagg agtccccgta gttggtgtag ctgttgctgc
     6781 aattatggtt gctttattag ttatccaaac taaacgcaat cctgtaaata tttaaaggga
     6841 gagtgataat tatgggagat tctaaattaa ctaaaaaaga aataaacaag gttttctggc
     6901 gttcatttgc tttacaggga tccgagtcat acactgatat gcaaggaatg ggatatagtt
     6961 tttctatatt acctgcttta aacaaaatat atcaagggaa tgatgaaaaa tattatgaag
     7021 ctgtttaccg aaatacggaa ttttttaata caacgcctca tatttcaacc tttattatgg
     7081 gtatgtcatt agcacttgaa gaagaaaatg caaaaacaga tcaatttgaa actgaaacaa
     7141 tttcatcgat aaaagctgca ttgatggggc ctgtagctgg tattggtgat agtttttact
     7201 ggggaacatt tcgcgttatt gctgctggca tcggcttatc tttagctgcc caaggaaaca
     7261 ttttaggtcc aatattgtat ttttcaattt atacgctgtt acattttctt ggcagatatg
     7321 gctgcttacg tattggatat cggatgggag caaacgccat tgaaaaagca tttgagaaca
     7381 attaccttga aagtttctcg ttagcagcat ctatcgtagg tttaatgtca attggagcca
     7441 tgacctcatc tctggtacgc tttactattc ccgccgtatt taaatcaaaa ggtggagaaa
     7501 taaatgttca gcaaatgctc gatgatattt ttccaggaat tctctcattg gggctaacat
     7561 tctttgttta ctttctatta aagaaaaaag taaaggttct ttggattata cttgctatct
     7621 tcgtctttag tattgttgga gtttatattg gattattagg ttaattaatc cagaataaaa
     7681 ctagatcaga catcaactta aacgattacg tccatataat tgtgtaaaag tgaagcatca
     7741 aaaaagccat tgatctcagt taggagtaca atgttttttg cagaaaacat actcgaatag
     7801 gactgatatc aatgactcaa gtacatttta cttttgaaag cgaagaaatt caagcaatta
     7861 taaacgaaag cggtgcgaat gataccgcaa aaacacttat gactatcatg tttaatcagc
     7921 tgatggaaga acaacgaaat caatatattc aggcaaatgc ctatgaacgt tcagaagaac
     7981 ggcaatcaca aagaaatggt tactacgatc gttcgtttac aactcgaatt ggtacgttag
     8041 aacttcatgt tccacgaacc agagacggta agtttagtcc tactatcttt gaaagatatc
     8101 agcgaagcga aaaagctttg atcgctgcca tgattgaaat ggtcatttct ggtgtctcca
     8161 ctcgcaaagt aactaaaaca gttgaactgc taacggatgg tgcgactgtt tctaaatcat
     8221 tcgtttcaaa tctgatgaaa cagttggatc catttgtttt tgagtggaga aaccggagcc
     8281 tagaaggctc ggaatatcca tttttcatgt gtgatgccct gtatatgaaa gtaagggaaa
     8341 atcatcgtat tgtctcaaaa ggtgtatata ttggtatcgg cattgattct gatggacgac
     8401 gtacgattct tggttttgat gttcaagacg gcgaatcgga agataattgg gataccgttt
     8461 ttcaatcgtt cgttcaacga ggtttgttcg gtgtaaaact ggtcatttct gatgcacaca
     8521 aagggttagt taaggctgtt cgcaagaact ttttaggtgc gagctggcaa agatgccaag
     8581 cccacttttt gagaaatatt tttgataagc tgccaaagaa agtttcttct gatgtaaaag
     8641 acgagttgaa gagtatcttt aaagcctctg aacttgaatt gacacgtgaa agaaaagagc
     8701 acttcttgga aaaatatggt tgtgattcaa aattaagtgc tgcttgcgac attttagaaa
     8761 acggctttga agatgctatt caaattttgt cttttcctga aaatatccgc cgaaggattc
     8821 gtacaaccaa tgtgttggaa cggttgaacg aagagattcg ccggagagaa cgagtgattc
     8881 ggatttttcc aaatatcaat tccatcactc ggataattgg aacacttttg atggaaaaag
     8941 acactgagtg gctggcttct ccacgaaaat atttagaatt taattcgaat aacatttagc
     9001 accatgaacc tgtgcgagtc tcttctttct gaggagagac atcaagagtc gctgcgctac
     9061 tgctcctgac ctctctcctc agaaagaaag gcatggcagt taggttcaca acgcaaaaca
     9121 ttgtgctttt gatgaggttg aacttttaca caagattgtg gacttgactt agtatcattt
     9181 atttttttaa aacactagat tagtcactat ataaaatgtg attaatttta ttattaataa
     9241 aatatttttt atattacaaa agtagtgttt ca
//
LOCUS       DACDCJ010000099         8899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDCJ010000099 DACDCJ010000000
VERSION     DACDCJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.100"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..267
                     /locus_tag="IV038_002509"
     CDS             <1..267
                     /locus_tag="IV038_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342673.1"
                     /translation="LKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAKGIYE
                     MKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(364..798)
                     /locus_tag="IV038_002510"
     CDS             complement(364..798)
                     /locus_tag="IV038_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8342674.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1030..1740)
                     /locus_tag="IV038_002511"
     CDS             complement(1030..1740)
                     /locus_tag="IV038_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP8342675.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1730..2590)
                     /locus_tag="IV038_002512"
     CDS             complement(1730..2590)
                     /locus_tag="IV038_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP8342676.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2595..3233)
                     /locus_tag="IV038_002513"
     CDS             complement(2595..3233)
                     /locus_tag="IV038_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP8342677.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3248..3547)
                     /locus_tag="IV038_002514"
     CDS             complement(3248..3547)
                     /locus_tag="IV038_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8342678.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3590..5074)
                     /locus_tag="IV038_002515"
     CDS             complement(3590..5074)
                     /locus_tag="IV038_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP8342679.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5094..5555)
                     /locus_tag="IV038_002516"
     CDS             complement(5094..5555)
                     /locus_tag="IV038_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8342680.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5573..6640)
                     /gene="ulaG"
                     /locus_tag="IV038_002517"
     CDS             complement(5573..6640)
                     /gene="ulaG"
                     /locus_tag="IV038_002517"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP8342681.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6858..7628
                     /locus_tag="IV038_002518"
     CDS             6858..7628
                     /locus_tag="IV038_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8342682.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7901..8728)
                     /locus_tag="IV038_002519"
     CDS             complement(7901..8728)
                     /locus_tag="IV038_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342683.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2704 a         1871 c         1388 g         2936 t
ORIGIN      
        1 cttaagcaat ttccacaaga agaacatttc atgtataaag aagcaatttc ccacaaagtg
       61 acagagtatt tggagcataa tttttttcgt ataaagccag tgcgtactcg ttcggcaaaa
      121 ggaatatacg agatgaaagt caaaattaat aaaaaaaatt atcgcatagc atttgcttta
      181 aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat tgtatttgat
      241 aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga gtgatactaa
      301 gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt acgttattat
      361 atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga cataagaaat
      421 accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt ttgttttctc
      481 agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct tctccaaagc
      541 ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa tgaggatatc
      601 ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga taatcttccc
      661 ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta accttgcagt
      721 gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct catcgatcat
      781 aagggaatca atattcattt ttttcttcct ttcttataca attctccctc taataataca
      841 gttctttgac tatttaatct atcacatata actctcctgt aaagattctc tcgattcctt
      901 aaaatctctc tttcctttta gaatgcatcc tttttgataa ataaaaaggt tgtgataaat
      961 tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc tacccttacc
     1021 ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat aatgtttatc
     1081 tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt ctttggccat
     1141 cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg aatcccccca
     1201 tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat ctaattttct
     1261 ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt ctcttacttc
     1321 ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag catgagtcgt
     1381 gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg tggaatgagt
     1441 atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg tggggagatc
     1501 tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg ttaccaccat
     1561 ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga tgactcctag
     1621 ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt atcgcggcaa
     1681 tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt cacaagacat
     1741 catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg atttctcttc
     1801 gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca taatctagac
     1861 gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa ggaacatcac
     1921 gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg atagcagtga
     1981 tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat gcggacaaat
     2041 tgcctaggtc tggatatact tgtagaaaag gggaatgaat ctgttctttg atttctaaaa
     2101 attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga atgatgccat
     2161 atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct ctggaattca
     2221 ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg acgcttagat
     2281 tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc ttttctggat
     2341 cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg atacgtacac
     2401 cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa tctaatcgtg
     2461 ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc atttctttta
     2521 caagggaaaa acgttctttc catgaaatat ctttgggtaa tgctttttca taaatcccaa
     2581 tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt ttgtgcggcc
     2641 ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata cacatcgatc
     2701 ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac attaaagcct
     2761 agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc accagataac
     2821 agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg ccaatccatg
     2881 gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat ttctttttgt
     2941 gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc tgctcctgct
     3001 tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc agcgactagt
     3061 tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc ctgtaagcaa
     3121 agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac atctcctaat
     3181 gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct catcttagtt
     3241 ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca ctgatttctt
     3301 tggctgacat cacatttttg ataccgataa ctgtaacttc ttttttctct gcctgagtaa
     3361 acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct gaactttttc
     3421 cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct ttcattgctt
     3481 tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac gatactaaaa
     3541 ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt tatactctct
     3601 gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccctca ggatttttcc
     3661 gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt ccagcataac
     3721 ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg aacatcccga
     3781 tataaccacc atatgttgac atcccaatcc atgtagcgaa taatgctgaa ccgcctactt
     3841 gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct cggttattgg
     3901 cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct atgacgatcg
     3961 ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa gcaccaaaca
     4021 agaacccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct acgtcaattc
     4081 ctggaacagc tcctggtaaa attgtgtttg agattccttg gaaagattga gttagttcat
     4141 cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa tttaaagaag
     4201 tcgtcataat gtagaataag aaactctgtc cttcttgcat gaattgcgct tgaatcaaat
     4261 aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt aacgaagtag
     4321 caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct tcgatttttt
     4381 tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac tcagctaatt
     4441 ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg gcaccttctg
     4501 tcaattcctg cgtgattcct actgttaagt tagaagctac tgcccaataa caacctaaaa
     4561 tcagtcccat aaaaatcaat acttgtatcc tacctagatc aggaaagcaa aacagtaaaa
     4621 tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa acggaacgca
     4681 gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca atcagtaata
     4741 aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca tccacagcat
     4801 tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc tttagtccaa
     4861 ccaaaatcgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa ataaagtaac
     4921 cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc agcaaaatat
     4981 agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata tttgttgcaa
     5041 aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat caattaatta
     5101 tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag acgctcaacc
     5161 aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg ttcttctgga
     5221 tcttttgctg caagtgtaaa aaacaaacta gccgttttct cctcatcctc cccttcactt
     5281 tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa aacaccatca
     5341 cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata aggaccgtat
     5401 ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc cttatcgatc
     5461 aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt atctgggtaa
     5521 tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt tcttataaaa
     5581 acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct cgataaaaat
     5641 taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact tgccagatat
     5701 aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca gtgatctctt
     5761 ttgaatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact acttttgcat
     5821 tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt atccctcgtg
     5881 ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca tgtttggcaa
     5941 ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat gtttcgaata
     6001 aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat acctcttctg
     6061 gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact acattgatgt
     6121 ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca ggaattcccc
     6181 atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca ggacaatttt
     6241 gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt gtaacgatta
     6301 aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta cgtaaattag
     6361 gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc atcatgccat
     6421 tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc gttccttcat
     6481 gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt ccaggatcca
     6541 ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat gtttctacta
     6601 accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc cccccatatc
     6661 gttgttccaa cttacaagta aatattaatg tgtatcagca tccatggaaa cccttttata
     6721 tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc agtataataa
     6781 agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat caagatgtcc
     6841 ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt caaaaagaaa
     6901 ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct gaatcccttg
     6961 atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa aacgagatca
     7021 tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt gatcaatctg
     7081 gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt aacgattatg
     7141 atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt cttcaatcta
     7201 accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac aaagataatt
     7261 gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta gtgggcgaca
     7321 tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc tgtagcggag
     7381 tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc aacgaaatga
     7441 tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa atcggactga
     7501 catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact gataaatttt
     7561 gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt caagtagatt
     7621 cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg aataacaaat
     7681 ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca agatgtgtcc
     7741 atccaatcga taccatcata atctattgct tcttttataa ccgttatatg ttgcctaaat
     7801 aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc ttttttcgaa
     7861 taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg tcgttttcaa
     7921 tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg tccatatatc
     7981 tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca taaagcaagt
     8041 attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc tcacataatt
     8101 ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca taatatacaa
     8161 tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta cgctccacat
     8221 aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg tttgcagctg
     8281 cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta aaataaaata
     8341 atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa aataaaattt
     8401 gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa attttataag
     8461 attcttggta ttttgataca gaattcaatg attgaatctc atttattttt ttcctgattt
     8521 tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt ttgcaaagcg
     8581 cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg acagttgctt
     8641 gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt cggtattctt
     8701 ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaaaa aattatttct
     8761 cctttatatc atacacaaaa aaaagttcta aacaaccaaa aatgtttaga acagaataaa
     8821 aattttaatt ttaaaataat attcaaatta aacaactggc gtatctgctc cttgatttct
     8881 ttttgtgtta tctgaataa
//
LOCUS       DACDCJ010000100         8526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDCJ010000100 DACDCJ010000000
VERSION     DACDCJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.101"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            488..913
                     /gene="lacA"
                     /locus_tag="IV038_002520"
     CDS             488..913
                     /gene="lacA"
                     /locus_tag="IV038_002520"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP8342684.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            930..1445
                     /gene="lacB"
                     /locus_tag="IV038_002521"
     CDS             930..1445
                     /gene="lacB"
                     /locus_tag="IV038_002521"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP8342685.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1456..2388
                     /gene="lacC"
                     /locus_tag="IV038_002522"
     CDS             1456..2388
                     /gene="lacC"
                     /locus_tag="IV038_002522"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP8342686.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2391..3371
                     /gene="lacD"
                     /locus_tag="IV038_002523"
     CDS             2391..3371
                     /gene="lacD"
                     /locus_tag="IV038_002523"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8342687.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3399..3716
                     /locus_tag="IV038_002524"
     CDS             3399..3716
                     /locus_tag="IV038_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP8342688.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3716..5422
                     /locus_tag="IV038_002525"
     CDS             3716..5422
                     /locus_tag="IV038_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8342689.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5564..6970
                     /gene="lacG"
                     /locus_tag="IV038_002526"
     CDS             5564..6970
                     /gene="lacG"
                     /locus_tag="IV038_002526"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP8342690.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6993..7265
                     /locus_tag="IV038_002527"
     CDS             6993..7265
                     /locus_tag="IV038_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP8342691.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            7286..8185
                     /locus_tag="IV038_002528"
     CDS             7286..8185
                     /locus_tag="IV038_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP8342692.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         2718 a         1439 c         1746 g         2623 t
ORIGIN      
        1 tgaagaccct tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc
       61 ataaaagaca atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg
      121 atgaaataag aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa
      181 aataaagtag gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg
      241 aatagatatt tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta
      301 acggtgttat ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaaagtg
      361 ttgctttttg tttgaatgct tgatattata taaacaaaga aatgatgaaa acgttttttc
      421 gaacattttg caaaatgttt tctacttcta cgtagcattt ctttctaaaa tttaggaggt
      481 ggtcaaaatg gctattgttg ttggtgcaga tctcaaaggt acgagattaa aagatgttgt
      541 gaaaaatttt cttgtagaag aaggttttga ggtaattgat gtgactaagg acggacaaga
      601 ttttgttgat gttaccttgg cagttgcctc tgaagtaaat aaagatgagc aaaacttagg
      661 tattgtaatt gatgcttatg gagctggtcc attcatggta gcaactaaaa ttaaaggtat
      721 ggttgcagca gaagtttcag atgaacgctc agcttatatg acacgtggac ataacaattc
      781 acgaatgatt acggttggcg ctgaaatcgt cggtgatgag cttgctaaaa acatcgctaa
      841 aggtttcgtc aatggtaaat atgatggcgg acgtcatcaa atccgagtag atatgcttaa
      901 caaaatgtgt taaggaaagg agttaaggaa tgagaatcgc aattggatgt gaccacattg
      961 tcacagatga aaaaatggca gtatcagaat tcttgaaatc aaaaggacat gaggtccttg
     1021 atttcgggac atatgaccac gcacgtactc actaccctat ctatggtaaa aaagtcggtg
     1081 aagcagtagt aagtggtcaa gcagacttgg gagtatgtat ctgtggtaca ggtgtcggca
     1141 ttaacaatgc agtcaataaa gttccaggtg ttcgctcagc gttggttcgt gatatgacat
     1201 cagctatata tgcaaaagaa gagttaaatg cgaatgttat cggtttcggt ggaaagatta
     1261 ctggtggtct tcttatgaat gacatcattg aagctttcat tgaagctgag tacaaaccaa
     1321 cagaagaaaa taaaaaattg attgcaaaaa tcgaacacgt tgaaacacat aatgcacatc
     1381 aagcagatga agatttcttt acagaattcc ttgaaagatg ggaccgtggt gactaccacg
     1441 attaaggtat aacaaatgat tctgacagtc acactaaatc cttcagtaga catctcttat
     1501 cctctagaaa cattgaaaat tgatacagtg aatagggtga aagatgttag taagacagct
     1561 ggtggaaaag gcttaaatgt tactagagtc ttgtatgaat ctggtgataa agttactgct
     1621 acaggtttct taggtggtaa aattggtgaa tttattgaaa gcgaattaga acaatctcct
     1681 gttagtccag ctttttataa aatctcagga aacactagaa actgtattgc tatcttacac
     1741 gagggtaacc aaacagaaat tttggaacaa ggaccaacag tttctcatga agaagcagaa
     1801 ggttttcttg accactatag caatcttatt aaacaatcag aagttgtgac aatttcagga
     1861 agtttacctt caggacttcc caacgattat tatgaaaaac ttatccaact tgcttcggat
     1921 gaaggagtag cagtcgtttt ggattgttca ggtgcacctc ttgaaacagt tctaaaatcg
     1981 tcggctaaac ctactgccat caaacctaat aatgaggaac tttcacagtt gcttggaaaa
     2041 gaagtaacaa aagatattga agaactcaaa gacgttctta aagagtcact ttttagtggt
     2101 atcgaatgga tagtggtctc attaggtcga aacggtgcct ttgcaaaaca tggtgatgtt
     2161 ttctataagg tagatattcc tgatatccca gttgttaatc cagtcggatc aggggactca
     2221 acggtagctg gtattgcatc agctttaaat agtaaaaaaa gtgacgctga cttattaaaa
     2281 catgcgatga cattgggtat gttaaatgct caagaaacaa tgacagggca tgtgaatatg
     2341 actaattacg aaacactaaa ctcacaaatt ggagtaaaag aggtataaaa atggtactta
     2401 cagaacagaa acgcaaatct ttggaaaaaa tttcagataa aaacggtgtt atctcagctt
     2461 tggcatttga ccaacgtggt gctttgaaac gtttgatggc acagtatcaa gatacagaac
     2521 caactgtagc tcaaatggaa gaactaaagg ttttggttgc tgatgaactt acaaaatacg
     2581 cttcttcaat gttgcttgac cctgaatatg gacttccagc aacaaaagca ttggataaag
     2641 aagcaggtct tcttcttgcc tatgaaaaaa caggttatga tacatcaagt acaaaacgtt
     2701 tgcctgactg cttggatgtt tggtctgcaa aacgtatcaa agagcaaggc gctgatgccg
     2761 ttaagttctt gctttactat gatgtagaca gctcagatga acttaaccaa caaaaacaag
     2821 cttatattga acgtgttggt tctgaatgtg tcgctgaaga tattcctttc ttcttggaaa
     2881 tccttgctta tgatgaagaa atctcagatg caggttctgt tgaatatgca aaagtgaaac
     2941 ctcgcaaagt cattgaagcg atgaaagttt tctctgatcc acgctttaac attgatgttc
     3001 ttaaagtaga agtaccagtt aacgttaaat atgtcgaagg ttttgctgat ggtgaagtgg
     3061 tttacagtaa agctgaagct gctgacttct ttaaagcaca agaagaagcg actaaccttc
     3121 catacatcta cctaagtgca ggtgtatctg ctaaattgtt ccaagaaaca ttgcaatttg
     3181 ctcacgactc aggtgccaag tttaatggtg tgctttgtgg acgtgctact tgggcaggat
     3241 ctgtagaacc ttacatcaaa gaaggtgaaa aagcagcacg tgaatggttg cgtactactg
     3301 gattcgaaaa tattgatgaa cttaacaaag ttcttgttaa aacagctagt ccatggactg
     3361 ataaagtata gtaaaaacat aaaacggagg atattgttat gaacagagaa gagatgactc
     3421 tcttagggtt tgaaattgtt gcttatgctg gagatgctcg ctctaaactt ttagaagcgc
     3481 ttaaagcggc tgaaaatggt gatttcgcta aggcagatag tcttgtagta gaagcaggaa
     3541 gctgtattgc agaggctcac aattctcaga cagctatgtt ggctcgagaa gcttctgggg
     3601 aggaacttcc atacagcgtt accatgatgc atggtcagga ccacttaatg actactgtct
     3661 tattaaaaga tgtgattcat cacctcatcg aactttataa aagaggagca aaataatgca
     3721 taaactcatt gaacttattg agaaagggaa accgttcttt gagaaaatct ctcgaaacat
     3781 ctatcttcgt gctattcgtg atggatttat cgctggtatg ccagtcatcc ttttctcatc
     3841 tatctttatc cttattgcct atgtcccaaa tgcttgggga ttccactggt caaaagacat
     3901 tgagaccttc ttgatgactc cttatagcta ttcgatgggt atcctagcat tctttgttgg
     3961 tggtactacc gctaaagctt tgacggactc aatgaaccgt gacctacctg cgaccaatca
     4021 gattaacttc ttatctacga tgctagcgtc tatggtaggg ttccttttga tggcagctga
     4081 gcctgcaaaa gaaggaggct tcttgacagc cttcacggga acaaaaggtc ttttgacagc
     4141 atttattgca gcctttgtga cagttaatgt ttacaaggtc tgtgtgaaaa ataatgttac
     4201 tattcgtatg cctgaagagg ttccaccaaa tatctctcaa gtatttaaag acttgattcc
     4261 attcactgta gcggttgttc tcctttatgg atttgaactc attgttaagg gaactcttgg
     4321 tgtaaccgtt gcagaatcaa tcggtaccgt tcttgctcct ctcttctcag ctgcagatgg
     4381 ttaccttgga attacactta tctttggtgc ttatgccttc ttctggtttg ttggtatcca
     4441 cggtccttca attgtcgaac cagcaatcgc tgctatcact tatgccaata tcgatgccaa
     4501 cttgcatctt atccaagctg gacaacatgc agataaagtt atcacttctg gtactcaaat
     4561 gtttattgtt accatgggtg gaacaggagc tacattgatt gttccattct tgttcatgtg
     4621 gatttgtaaa tcagaacgta accgtgccat cggacgtgcc tcagttgttc caacattctt
     4681 tggggttaat gagccaatct tatttggtgc accaatcgta ttgaatccga ttttctttgt
     4741 accgttcatt ttcgctccga ttgtcaacgt ttggatcttt aaattctttg ttgacacatt
     4801 gaacatgaac tcattctctg ccaaccttcc atgggttact cctggtccgt tgggaattgt
     4861 actcggtact aacttccaag ttctatcatt tatcttagcc ggactcttgg tagttgttga
     4921 tactatcatt tattacccat ttgttaaggt atacgatgaa caaattcttg aagaagaacg
     4981 ttctggtaaa acaaacgatg ctcttaaaga aaaagtagct gcaaacttca atactgctaa
     5041 agcagatgcc gttcttggaa aagcaggcgt tgctaaagaa gatgtagcag caaacaataa
     5101 tatcacaaaa gaaactaatg tccttgttct ttgtgcaggt ggaggtacaa gtggcttgct
     5161 tgccaatgcc ttgaacaaag cagcagcaga atacaatgtt ccagtcaaag cggcagcggg
     5221 tagttacggt gctcaccgtg aaatgttgcc agagtttgac ttggttatct tggctccaca
     5281 ggtcgcttca aacttcgatg atatgaaggc tgaaacggat aaattgggca ttaaacttgc
     5341 aaaaactgaa ggagctcaat atatcaaatt gacacgtgat ggacaaggtg ctcttgcatt
     5401 tgttcaacaa cagtttgatt aataaatttg taaaagttat ttgagacgag attattcttt
     5461 cagttcctta taatattgtt aaccatatca ttgcatttca tttccttggt ttccggaact
     5521 cagaatatcg tctcaaatgc tttttttgaa aggatttata gttatgacta aaacacttcc
     5581 taaagatttt atttttggtg gtgctacagc tgcttatcaa gcagaaggtg cgactcacac
     5641 ggatggtaaa ggcccagttg catgggataa gtatcttgaa gacaattatt ggtacacagc
     5701 agagcctgca agtgattttt accataaata tcctgttgat ttagaattag ctgaaaaata
     5761 tggcgttaat ggtattcgta tttcaattgc ctggtctcgt attttcccaa ctggctatgg
     5821 cgaagtaaat gaaaaaggtg ttgaattcta ccataatctt tttgccgagt gccacaaacg
     5881 tcatgttgag ccattcgtga ctttgcacca ctttgacaca ccagaagttc ttcactcaga
     5941 tggagatttc ttaaaccgcg aaaatataga acactttgta gattatgccg ctttctgttt
     6001 tgaagagttc ccagaagtaa actactggac aactttcaat gaaattggcc caattggaga
     6061 tggtcaatac ttggttggta aattccctcc aggcattcaa tatgatcttg ccaaagtctt
     6121 ccaatcacac cacaatatga tggtttctca tgcacgtgct gtgaaattgt ataaagataa
     6181 aggttataaa ggcgaaattg gtgtagtaca tgcgttgcca accaaatacc cttatgatcc
     6241 agaaaatcca gcagatgttc gtgccgctga gttagaagat atcatccata acaaatttat
     6301 cttggatgca acctaccttg gacactattc agataaaacg atggaagggg tcaaccacat
     6361 cctagctgaa aatggtggag aacttgatct tcgtgatgaa gacttccaag cacttgaagc
     6421 agctaaagat ttgaacgatt tccttggtat caactactac atgagtgatt ggatgcaagc
     6481 ctttgatggt gagactgaaa tcattcacaa tggtaagggt gaaaaaggaa gttctaagta
     6541 ccagattaaa ggtgttggtc gccgaatcgc accagattat gtgcctagaa cggattggga
     6601 ctggattatt tatcctgaag gcttgtatga ccaaatcatg cgagtgaaaa atgattatcc
     6661 aaactacaag aagatttaca tcactgaaaa cggtcttgga tacaaagatg agtttgtaga
     6721 taataccgtt tatgatgatg gtcgtattga ttacgtgaag aaacacttgg aagttttatc
     6781 agacgcaatt gcggatggtg caaatgttaa aggttacttc atctggtcac ttatggatgt
     6841 cttctcatgg tcaaacggat acgaaaaacg ttatggacta ttctatgtag actttgacac
     6901 gcaagaacgc tatcctaaga aatcagctca ttggtataag aaagtagcag aaactcaagt
     6961 gatagagtaa tattacttga aaatgtaaaa aattgttatc cgatacttta gaaaaagtac
     7021 aacacttatt tagaccaggc tttgaaaagg tatatattgt tagtttcgaa gattgtccta
     7081 tgattcctga gcttgaagct accccgttgc taaaatgtgg taaatggtat gtttcaactg
     7141 gaaaagagtg gatttgccat tctgatttag agttatcagc atttgaatgg gaatttttac
     7201 aatcactcga tgtagaaata cgtgagacta ttcacttcga ggtaaattat ttgcctttcc
     7261 aataataaaa agaaaggaat tagttgtgac tatcgaatta aaaaacgagt atcttactgt
     7321 tcagtttaaa acacttggtg gtcaactgac ttcgattaaa gacaaggatg ggatagagta
     7381 tctctggcaa gctgatccaa attattggaa tggacaggcg cctattctat ttcctatctg
     7441 tggtagttta cgaaatgatt gggctattta tagacctcaa gaaagacctt tttttacagg
     7501 tcttattcgt agacatggtt ttgttcgaaa agaagaattt attctggaag aagtcaatga
     7561 taatagcgtg acctttagta ttaaaccaaa tgctgagatg cttgataatt acctatatca
     7621 gtttgaactg agagttgttt ataccttgaa tggtaaatca attagaaccg aatttcaggt
     7681 aacgaacttg gaaatggaaa aaacaatgcc ttactttatt ggagcccatc cagcattcaa
     7741 tagtccttta gtagaaggtg aaaactatga ggattattat attgaattta gtgaggttga
     7801 gtcttgctcg ataccaaaga gttttcctga gacaggatta ttagatttgc aagatcggac
     7861 accttttttg gaaaatcaaa aaattctcga tttggactac tcactttttt cccatgatgc
     7921 cattacactt gaccagctca agtctcgtag cgtaacgctt cggtcaagga agtctggtaa
     7981 aggtcttcga gtagattttg atgattttcc gaacttgatt ctgtggtcta caactaataa
     8041 aagtccattc attgctttgg aaccttggag tggtctctcc acttcacttg aagaaggtaa
     8101 tttcttagaa gataaaagac aggtaacgaa aatttcacct caagaaactt ctagaaaaat
     8161 ctatgatatt actatcctta attaaaattt gttattattt gagtcttcaa taagatttga
     8221 atactcttac cttgtgatga tagattggtc ttctccgtgc cagtcttaca tcgcaaggtt
     8281 tttaagtttt ttgtgggtct atgtcaactg tagtgggtga cgaaaagcta atatctaaag
     8341 acagtctggt agtaaaaaat aagattattt actacttctt gatttaaaat aatagtggag
     8401 ccattaattt gtggcaaatt taatttaata ttttgcctca aattagccac actttttata
     8461 gaactaagag gaaaaatgaa attttcttcc tattaatata gttgtttgaa atcttaaata
     8521 tagcgt
//
LOCUS       DACDCJ010000101         8430 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDCJ010000101 DACDCJ010000000
VERSION     DACDCJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.102"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(177..971)
                     /locus_tag="IV038_002529"
     CDS             complement(177..971)
                     /locus_tag="IV038_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAP8342693.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1590..4424
                     /locus_tag="IV038_002530"
     CDS             1590..4424
                     /locus_tag="IV038_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8342694.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4615..5106
                     /locus_tag="IV038_002531"
     CDS             4615..5106
                     /locus_tag="IV038_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP8342695.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5209..6198
                     /gene="manX"
                     /locus_tag="IV038_002532"
     CDS             5209..6198
                     /gene="manX"
                     /locus_tag="IV038_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP8342696.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6231..7034
                     /locus_tag="IV038_002533"
     CDS             6231..7034
                     /locus_tag="IV038_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP8342697.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7055..7969
                     /locus_tag="IV038_002534"
     CDS             7055..7969
                     /locus_tag="IV038_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP8342698.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8052..8426
                     /locus_tag="IV038_002535"
     CDS             8052..8426
                     /locus_tag="IV038_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP8342699.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
BASE COUNT         2741 a         1396 c         1739 g         2554 t
ORIGIN      
        1 ccacacctta taaaaatctt tgacaaatct attcgaagga tcgaatttaa accataattg
       61 tgtttttcct tccattaaca tataaagttc acctcaaaaa gagtatacga acaaatgttt
      121 gtattgtaaa tagaaaaaga tgagctaaat taaatgccca tcttctttga tagatattat
      181 aaatctaata attcttgttt ctttttatta aattcttctt ctgtaatgat tccatcatcc
      241 aatagttctt tatattttct tatttctgaa gctgaggaaa ttgttgatgt attggaattt
      301 gtagcagaaa tatttcttct ttttaaatta taaataattt cgtttaaaga atcacttatc
      361 ctatcaacag tatctttatc aactgcaaca ttcaatactt taccaagagt atcaatagtt
      421 aatgtaccca acagcactcc tcttttttta ctaatattat ttaaattgtc taatataata
      481 ctttgtacat tattgcctat cattttttgt tgagctacaa ttaaacgttt attagttaat
      541 gcgtatgcat aattattgtc atgcttagtt gatgagatat aattatgcaa tcctacaaag
      601 cagaagatta cgtattcgtc actgttaagt tgatctgata ttaagttaaa atgatgagtt
      661 gtccattttt tagtcattcc tttaccgtag ccgtatttaa gacaaaattc taccatctct
      721 tcagtggttc tcattctatc attacctata tcatgattgt tttgtgcagt atgatcagat
      781 atgtttaatt ttcttttttc tgatggatcc ttatctaaat cttctaaatt caaagatctt
      841 attattttat aaggagtagt cataccgtaa ccagcttttt tcatacaatt gccgcaaaca
      901 atttgtttat ctttgatttt atatgaagtt aagccaattt tacctccgca ctttgcacat
      961 tgtttactca cattagttcc tcctagatat cagctttcta ctactattaa cattctatca
     1021 atagtgacca tactttgacc atacttttta gaaagaatag aaaattccaa gatatttaat
     1081 aatagaagaa aagcactaac atcatgggtt cagaagaatt gagagcttat caggaaacag
     1141 tataattgaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     1201 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     1261 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     1321 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     1381 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     1441 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     1501 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     1561 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     1621 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     1681 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     1741 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     1801 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     1861 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     1921 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     1981 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     2041 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     2101 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     2161 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     2221 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     2281 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     2341 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     2401 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     2461 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     2521 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     2581 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     2641 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     2701 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     2761 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     2821 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     2881 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     2941 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     3001 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     3061 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     3121 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     3181 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     3241 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     3301 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     3361 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     3421 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     3481 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     3541 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     3601 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     3661 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     3721 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     3781 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     3841 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     3901 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     3961 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     4021 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     4081 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     4141 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     4201 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     4261 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     4321 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     4381 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     4441 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     4501 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     4561 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     4621 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     4681 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     4741 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     4801 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     4861 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     4921 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     4981 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     5041 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     5101 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
     5161 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
     5221 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
     5281 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
     5341 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
     5401 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
     5461 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
     5521 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
     5581 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
     5641 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
     5701 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
     5761 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
     5821 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
     5881 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
     5941 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
     6001 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
     6061 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
     6121 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
     6181 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
     6241 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
     6301 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
     6361 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
     6421 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
     6481 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
     6541 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
     6601 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
     6661 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
     6721 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
     6781 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
     6841 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
     6901 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
     6961 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
     7021 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
     7081 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
     7141 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
     7201 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
     7261 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
     7321 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
     7381 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
     7441 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
     7501 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
     7561 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
     7621 agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt
     7681 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
     7741 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
     7801 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
     7861 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
     7921 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
     7981 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
     8041 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
     8101 cgtttacggg tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt
     8161 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
     8221 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
     8281 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
     8341 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
     8401 ttcgtagtat gtttaaacgc aagtaaaaaa
//
LOCUS       DACDCJ010000102         8235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDCJ010000102 DACDCJ010000000
VERSION     DACDCJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.103"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            620..1483
                     /locus_tag="IV038_002536"
     CDS             620..1483
                     /locus_tag="IV038_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8342700.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1612..3234)
                     /locus_tag="IV038_002537"
     CDS             complement(1612..3234)
                     /locus_tag="IV038_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8342701.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3483..4004
                     /locus_tag="IV038_002538"
     CDS             3483..4004
                     /locus_tag="IV038_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342702.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4190..5920)
                     /gene="spxB"
                     /locus_tag="IV038_002539"
     CDS             complement(4190..5920)
                     /gene="spxB"
                     /locus_tag="IV038_002539"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP8342703.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6085..6903
                     /locus_tag="IV038_002540"
     CDS             6085..6903
                     /locus_tag="IV038_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP8342704.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7006..7203)
                     /locus_tag="IV038_002541"
     CDS             complement(7006..7203)
                     /locus_tag="IV038_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342705.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7372..7950
                     /locus_tag="IV038_002542"
     CDS             7372..7950
                     /locus_tag="IV038_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP8342706.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2455 a         1506 c         1623 g         2651 t
ORIGIN      
        1 cgtgtcacag tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa
       61 gcccaatcag atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg
      121 cagagcagct ttttcacaac ctctccacta aacggtgttc tcctgccaac tcctcggtca
      181 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
      241 gagctaaacg gcaggctcac aacctcttgg taaacggtgt tcaatcagct gaccctcggt
      301 attagctcaa aaaacggcaa aatatgagga actatttttt ccgttttttt ctctaatact
      361 caggtcaaaa cgctgatttc acaacctctg attcacggtg ttccagaagt agcttctacg
      421 gaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
      481 cttgaagctg accacttctg tcacaacctc ttattttgat tagaacttgc gaaaataaac
      541 cggttaaagt gtgtctagcg caagagattt gctataatag acgataaggg taaaatgtaa
      601 cgagaagggg ttgaaaaatg tgtatcaaat tgtaacagat tcttgttgtg atctgccata
      661 ccatgtatta gcaaaagaaa aagttgattt tttatcaatg aatctccaat tgaatgatca
      721 agagttagta gatgacctcg gaaagacatt caattatgac cgctacatga atcagctgaa
      781 aaatggtgcg atgcctacga catcacaaat caatgttggc cgatatttag aatttttccg
      841 ttcttatgcg gaaaaacaga ttcctgtttt atatgtggct ttttccagtg gattaagcgg
      901 ctcttacagt agtgcgcttc aagcggttga attgctgaag gaagaatatg aagaccctga
      961 gatctatgtt tttgatacaa aagctgcaag tattggggaa gggcttttag tattggaagc
     1021 tgtaagattg aaaaaagaag gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa
     1081 ttatttgaaa gtccattcat gggtaacagt agacgatctg aaatatctag aacgaggcgg
     1141 taggatttcc aaagcaagtg ctactcttgg cagtctattg agtgtaaagc ctattattac
     1201 ggtggataaa gaaggaaggc tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat
     1261 ccagacaatc gtcaatgaga cggtaaaaac aatcgttgca ccacttgatc aaatcgttta
     1321 tgtggcttac gctggggatc tagagagcgc ggagaaggca aaacggcttt tggaagagca
     1381 gataaagatg aaagatgtga agctttatcc cttaggacca acgatcgcca gtcacacagg
     1441 ctatggctgt atcgcgattt tttcgatggg aatcagtcga taaaagcagt aactcattct
     1501 gtaaaaaagc ccagaaattg attgtaacaa aaacagcgac tcttaaaaga tagatgagca
     1561 tcctatctct tagaggtcgc tgttttttta ttttttaagc taaataggcc attatgccca
     1621 taactcgttc aattgttttt gaatatctgc attttccaaa aattcatcat atgtcgtatc
     1681 agctcgatcg atcacacctt tatccgaaac ggcaatgatg cggtttgcca atgtttggat
     1741 aaattgatgg tcatgtgacg caaataagat tgagccagta aatgccatca agccgtcatt
     1801 caatgctgtg attgattcta agtctaaatg gttcgttgga tcatctaata caagtacgtt
     1861 agctttcgaa agcatcattt ttgaaagcat gcaacggact ttctctccac cagataaaac
     1921 attcactggt ttcaatactt cttcaccaga aaacaacatt cttcctaaga agcttcttaa
     1981 gaaggtgtta tcatcttctt ctttacttgc atattgacgc aaccagtcta aaatcgttag
     2041 atcagtatcg aattctttcg ttgtatcctt tggtaaataa gaattacttg ttgtcacacc
     2101 ccaacgtact gttccagtat ctggcgtaat ttcacccatg ataactttga atagcgttgt
     2161 tgttgttata tcattttctg cgacgaaagc gaccttgtct tctttgctca aattaaaaga
     2221 tatatcatct aaaattttct ttccatcaat cgttacactt acattttcta cctgcaataa
     2281 gtcgtttcca atctcacgtt caggtttgaa tccaacgaat ggataacgtc tagaagaagg
     2341 ttggatatcg tctaaagtaa ttttttcaag cattttttta cgtgaagttg cttgttttga
     2401 ttttgatgca ttcgcactaa agcgggcaat aaattcttgg agttctttga tttgttcttc
     2461 ttttttggca tttgcgtttg cttgaagttt agccgctagt tgactagatt ccaaccaaaa
     2521 atcataattt cctacataaa gtttgatttt accaaaatct agatccgcca tatgtgtaca
     2581 tactttgttc aaaaagtgac ggtcatggga tacaacgatc acagtatttt caaaattgat
     2641 caaaaattct tccagccaat tgatcgattg gatatccaga ccgtttgttg gttcgtctag
     2701 caaaagaaca tcaggtttac caaatagtgt ttgtgctaat aacactttta ccttttgtcc
     2761 accagttaat tcactcattt gctgatggtg caattcttca gggatgttca gaccttgcag
     2821 taaaacagct gcttctggtt cagcttccca tccattaagc tcagcaaatt ctccttctaa
     2881 ttcagctgct cgaataccat cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt
     2941 ttctttcagc acttcataca gacgcttgtg tcccatgatg actgtttcaa gtacagtata
     3001 gtcttcatag tcgtaatggt tctgtttcaa tacggccatc cgttcatctg gtcccatagt
     3061 aacggaacct gtggaaggct gcagatcccc tgataacact ttaaggaacg ttgatttacc
     3121 tgcaccattt gcaccaatca agccatagca gtttccgggg gtaaatttaa tattcacatc
     3181 atcaaatagt ttgcggtctg aaaagtgcaa actcacatca tttacagtaa tcaatttttt
     3241 tcctcacttt tatcattatt agttttgaca agtcttatag aattatatag gggaaaggta
     3301 aaagattcaa ggcaatcgaa cattctttgt gtttcacgtg aaacagaatg aaaaaaataa
     3361 aaataaaagc ggagcgaaaa tagctactat aggatttatt agtttgcaac agcataattt
     3421 ttgacgttat ttataagagg gagtactata aaactgtaaa agaaatgaaa gaaggattga
     3481 aaatggatga aaaaagaacc atcgactggg gctcattgat tttgggaatt ttatttgtac
     3541 ttgtctcatt gttatcattt caagaccctg tcggaaattt agttgcaatt gtagtagtct
     3601 ttgcgatttt tgcctttatt aaagggatat ttgaactgtt tgtacggaac agattaaaag
     3661 agctaacagg atataaagga aagatgccct taataatcgg gatcatcgat attctagttg
     3721 gcgtcttttt cttattcaat attggtgcag gagtcgcagc attgcctttt gtttttgcag
     3781 tatggtttat tgctgattca gttttagcgt tatttactgc tgacttggca agaggagtaa
     3841 gtgaaggata ttattggttt acgatcatcg tcaatatttt aggaatttta ttaggaattt
     3901 tcttattgtt taatccaatt tcttctgcgt tgacattaag ctttttagtt ggattctatt
     3961 ttatgttatt tggtatcaca catatcgtct atgcatttag ataagtaaaa tcaaacaaat
     4021 aaaaaagccg gcttccgatt acagttttac tgttgtaatt ggaagccggc ttttttatca
     4081 tggacaaata tgcacgaggt gatttttagg tgtcgagaca aatttatgca aaaatgtgag
     4141 aagctatata cacaaatttt atcttaatac tcgaagcata gcgcagttgt cacaacctct
     4201 tttcatataa ttcattcaat actggcaaat catggacttc atatttttct ttgaatgctt
     4261 ggatttcttc ttcagaatat tttcttggat ctaaatgcag atcttctact ggcaatgggc
     4321 gcttgttatt gatcttcaca tcgattacta caggacgatc tgattttgca gctgcatcaa
     4381 aggcaaatcg aagttgcgag tattctgtga ctgtaaagct atttgccccg agcgcttctc
     4441 caactttacc gaaatctgca tcttctaaaa atacaccaaa tttcttttgt tccgtatctt
     4501 cttgttctgc ttcgatgaaa ccaaaagaat cattcgaaaa gacaacatta ataattggaa
     4561 gctgatattt gacttgtgta atgatatctt gcatgaccat agcaaacccg ccatcaccac
     4621 ttaaggtaaa tacttgtttt tctgggtagc ttagttgtgc ggcaattcct cctggcacac
     4681 cattacccat agttgcgaac catccagatg tcgtatgttt ttgtttaccg ttcatattca
     4741 ataaacgaat agaatggata gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt
     4801 cagcaatacg attgatttct ttatagacag cctctggacg aatcggttct tcctctcgat
     4861 cttcaaaaga tttcaaccaa tttacccatt tttctttatt ttcttgattt gctaggtacc
     4921 agctatcagc agggcgagca tcccctaact cgacaagtct ttgcaaagct gtgtttgcat
     4981 caccgaggat cgacaaagat gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga
     5041 tttggataaa ttcagcagtt ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga
     5101 caaataagat cagatcggct tctgccaatg cttcatttcc gggtttagta gcgacacgtc
     5161 cagcaaaacc aagaaagttt tcataatcat caggaataat tccttttgct agaacggaag
     5221 cgacaacagg aactgaaaaa tgttctgaga attctttgat ttgcggaaat ccgtttcgcg
     5281 ttccttgacc aacataaaga actggttttt ttgctttttc aagataaggc aaagcagcta
     5341 gcaaatcttt ttcttctggt aatatcacac ttgttttgtg agtgtgagcg gtggagaaag
     5401 gttgttcgtc aatttcttca aaaccaagat caacagggat agtcacgata gccacgcctt
     5461 tatgctcata ggcggcttta atggcttcat ctactacatg cggaagactt tgtggtgtca
     5521 tgactgtacg attgtatacg ctaacatctg cgaaaatagg attttcattt agttcctgga
     5581 aagagttata gttcatggat gtagaagcaa cttgtcctaa gaacgctact agaggcacat
     5641 ggtccatctg tgcatcatac aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc
     5701 cgaagacagc gccgactttc cccgttaatt ttgcatctgc agcggctgcg agtgcgccga
     5761 cttcttcatg acgaacttga atgtagttga tttcggtttt ttcttgatat agcgcatcca
     5821 taattgaatt gaaagatcct ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa
     5881 gaacttttac cattgcgatt cctgcattga tttttgacat ggtgtttcac tcctttagca
     5941 atgatgatac ataaattttt tgtatctaga gtatagaact ttttatcgaa aatcccaact
     6001 aattcgtctt ttcacaaaaa tgtagtcatg tttttgggaa tatgctagag tagtacatag
     6061 atagattcaa gagaggggaa tggcatggtt gaatggctat tgagacttga accttggcag
     6121 caagcacttg ctgggacagc ttttacttat tttatgacag ctcttggtgc cgggttggtt
     6181 tttttcttta aagaaatcaa aaaagaagtg ctaaacttga tgttaggatt tgcttctggt
     6241 gtcatgattg ctgcaagttt ttggtctttg ttagatccgg ctattacaaa agcagaagaa
     6301 aatggagata ttgcttggct agttgtaagt attgggtttg gtttaggcgg tgtgttttta
     6361 tatatggcag ataaaacact gccccatatg cattttggac cacaacatga agcagaagga
     6421 ttgcctaccc atttaaaacg gacgatcttg cttgtttttt ctattacatt gcataacatt
     6481 cctgaaggtt tagccgtggg ggtagcattc ggtgcagcag caactgcgga taatccgaca
     6541 gccgctatat tagccgctgt ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa
     6601 ggagcagctg tatcaatccc gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt
     6661 tacggacaag cttctggaat cgttgaacca attgctggag tgattggagc gctactagta
     6721 acgaaagtgg agctgctgct tccttatgca cttgcttttg ccgcaggagc aatgatttat
     6781 gtcgtggtgg aagaattgat ccctgaagca cagcagacat tgtccagcaa acgtcatttt
     6841 gcagtttttg gcgtcatgag tggatttatc attatgatga ttttggatgt ggcattagga
     6901 taaataaaaa aaatatatat gatatatcaa atagggccgc gaacttatat cgcgacccta
     6961 tttttttagt aagttatatc ataatgaagt gaatggtatc atacattaat aatagttttt
     7021 tctatgtgta tttcctttga tccagcgaac aagccagatg ataagtgcga ttgggattag
     7081 aactttgacc gcaaaccata aaagcccgct taggacactg acaaaaagac tgatgacgaa
     7141 gctagctgca attagcacta aaagaaccat gaagatattt aagaacatat ttttttgacg
     7201 catacaaaac cctcctcttg ttcattcttt actatagaaa aagtataaca gggattgttt
     7261 aaaaacacct ccaactgaag actgattttt acttcatcta cttaaatgat caaacaattg
     7321 ctttttctct ggtttatcat ctaaaataga gcggaaaagg aggagaatat catgcctaaa
     7381 aatcgtcttg aagcatttac agatgctgta atcgcgatta tcatgactat tttagtttta
     7441 gaacttcatg aacctaacgg agacacgtgg gaggcgctcg ctgcattagg ttaccgattt
     7501 tttatctata ttgtcagttt tatcatttta gcgatttatt ggaacaatca tcatcattta
     7561 tttcaagttg tacataagat aaatggacgg gtcttatggg cgaacagttt ctttgttttt
     7621 gcgctttcgc tttttccgtt tgcaacttca tggatcagtg agtacatcaa tagcctggct
     7681 ccagaaatga cttatggttt tgttaccttg accgctaata ttgcgtatta tatcttggca
     7741 aaagaacttg tcaaagcaaa taaaggccac gagaaagcat tgaaggcgat gttgaataat
     7801 tacaagaaat cgtatctatc gattggtttg aatgtgttag gaattgtctt agggtattta
     7861 gtagcgccag tagccgtgct gatcgcaaac atcgtgattc tagctgcttg gatcatccca
     7921 agtcgtacca tcgaatcaca atatcggtaa aatgatccgt gtttaaaatt taagagaaaa
     7981 atatagaaaa aataaataaa cgatcaaata tccgagtgat actagtattc aaagagggat
     8041 gcagatggat caaaaaagaa ttcatgtagg actgattact agttttgtca cgatgcttat
     8101 ttatttggat caaggtcatt tgacttggac agtagttggt gtcgtaggat ttttgacatt
     8161 attcacagca ataaaaaaat aaacatcttt atgtaatctg atgttctatc tatttaagaa
     8221 aagcatgttt acagc
//
LOCUS       DACDCJ010000103         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDCJ010000103 DACDCJ010000000
VERSION     DACDCJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.104"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IV038_002543"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IV038_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP8342707.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IV038_002544"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IV038_002544"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP8342708.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IV038_002545"
     CDS             2591..3376
                     /locus_tag="IV038_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8342709.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IV038_002546"
     CDS             3389..4240
                     /locus_tag="IV038_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP8342710.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IV038_002547"
     CDS             complement(4364..4816)
                     /locus_tag="IV038_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8342711.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IV038_002548"
     CDS             complement(5281..5649)
                     /locus_tag="IV038_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP8342712.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IV038_002549"
     CDS             5799..6281
                     /locus_tag="IV038_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP8342713.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IV038_002550"
     CDS             6318..7328
                     /locus_tag="IV038_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP8342714.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IV038_002551"
     CDS             complement(7443..8156)
                     /locus_tag="IV038_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342715.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACDCJ010000104         7933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDCJ010000104 DACDCJ010000000
VERSION     DACDCJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.105"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2008)
                     /locus_tag="IV038_002552"
     CDS             complement(<1..2008)
                     /locus_tag="IV038_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP8342716.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2024..3020)
                     /locus_tag="IV038_002553"
                     /pseudo
     CDS             complement(2024..3020)
                     /locus_tag="IV038_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3218..4459
                     /gene="sstT"
                     /locus_tag="IV038_002554"
     CDS             3218..4459
                     /gene="sstT"
                     /locus_tag="IV038_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP8342717.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4606..5241
                     /locus_tag="IV038_002555"
     CDS             4606..5241
                     /locus_tag="IV038_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP8342718.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5432..6172
                     /gene="truA"
                     /locus_tag="IV038_002556"
     CDS             5432..6172
                     /gene="truA"
                     /locus_tag="IV038_002556"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP8342719.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6317..7915)
                     /locus_tag="IV038_002557"
     CDS             complement(6317..7915)
                     /locus_tag="IV038_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP8342720.1"
                     /translation="MLFLSFAYVIPLFMSMGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2625 a         1234 c         1489 g         2585 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt atctgcagct
     3841 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     3901 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     3961 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     4021 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     4081 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     4141 agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     4201 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     4261 gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta ttatcggtgt
     4321 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     4381 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     4441 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     4501 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgctt
     4561 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     4621 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     4681 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     4741 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     4801 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     4861 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     4921 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     4981 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     5041 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     5101 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     5161 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     5221 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     5281 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     5341 ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     5401 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     5461 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     5521 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     5581 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     5641 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     5701 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     5761 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     5821 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     5881 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     5941 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     6001 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     6061 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     6121 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat aaaaatactt
     6181 tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata atgtatgaag
     6241 caaattaaag aaatataaaa aaagactttt tagcctgttt atgtgtacag ctaaaaagtc
     6301 tttcttaaat aaaaatctag tgagaatgtt gtacttctct tctcggttgt ttttgtttag
     6361 aaaaagtaaa tcgataaagt aaaaataaaa tcaaagtaac gatagataaa tatcttgaag
     6421 caattgctag ttttgtgtac tgataagtaa taacgacttt atttatacct ggaggaatag
     6481 ttaattcagt tgtgccaaac ttagataatt ttgtttgtac tagtttgcca ttaatagaag
     6541 ccacttgccc tttataacga taaataggtg tcgtcaagac agtattttta ttattcgaat
     6601 tatctaattc aattgtaagt ttgttactca taaaatgagt tgttggattt atttcctggt
     6661 tacctagtaa atattgatca ttattaatca tatcaggatg atagattgac tctttgtttg
     6721 cataatcagt atgattgtaa ttttcagcta tagcaacagc gtcttcaggt gctataagtg
     6781 ttaatgaatc tttgttagca tggatagaat tgcttactcc tgagagatgc aatattagtg
     6841 caagaaacgt aaagaccatt attttccatg aattattttt agtagaaaaa atataataga
     6901 tcactatact catcgtatag gctataaata aagaagaaaa accatttaac cgccagacaa
     6961 attgtagact cttaactggt gtatggttaa aaagttgcca aggaaataaa ttggttgaac
     7021 aaaaaagaac aaatacacca agaaagaaaa taaataaatc atcagcagtt aattttttta
     7081 taaaaataaa agcaccaatc aatccgagaa ataagaataa tccaatagta tagttgttat
     7141 agttattatt taaaatgtgt gtcagcatat cactaggagc cataccactt aattcttttc
     7201 ccagtgggac tttcaattct tgtgctctag attgttgaat aaaaggaatt aagaatcccg
     7261 cacttaaaaa aaaggtgagg acggtagctt ttaaaagact taagagtcta gcgatttttt
     7321 ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat aaatacagaa accatagcga
     7381 cagaaagtaa atgagtatac actactagcg tcatgccaat agacaaccag taccattttt
     7441 ggtaatctct aatataaatt tcgtaacacc ccattagtat caaaggcaaa aatgttaaag
     7501 cgacagcttc tcccaatgaa gcacgatgga atatgtcaat ggcacgatag gcggaaaatg
     7561 tataaataat cgaaaataac agcgaaatat aacgattgtt ttttatttgt ttcatagaaa
     7621 aataagaaac gaccattgtt aaaaaagtga taaaaaaata gtagatatta taacttaaaa
     7681 caagatttcc catcatttta taaaataaaa aagctggata aagagtaagc catggataaa
     7741 aaatattcat cattgttcca tgatgatcga agttattgaa gtttactgga gaactccata
     7801 cattatctag tcctatgatt cgagataaat gaaagtaagt atcctgattg acactatgat
     7861 agattcccat agacatgaat agagggatga cgtaagcaaa acttagaaat aacataaaaa
     7921 tgatatctat tat
//
LOCUS       DACDCJ010000105         7863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDCJ010000105 DACDCJ010000000
VERSION     DACDCJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.106"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1495)
                     /locus_tag="IV038_002558"
     CDS             complement(<1..1495)
                     /locus_tag="IV038_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342721.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYT"
     gene            complement(1515..1682)
                     /locus_tag="IV038_002559"
     CDS             complement(1515..1682)
                     /locus_tag="IV038_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342722.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            complement(1719..2408)
                     /locus_tag="IV038_002560"
     CDS             complement(1719..2408)
                     /locus_tag="IV038_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAP8342723.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2663..2983)
                     /locus_tag="IV038_002561"
     CDS             complement(2663..2983)
                     /locus_tag="IV038_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342724.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3016..4134)
                     /locus_tag="IV038_002562"
     CDS             complement(3016..4134)
                     /locus_tag="IV038_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342725.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4163..4957)
                     /locus_tag="IV038_002563"
     CDS             complement(4163..4957)
                     /locus_tag="IV038_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342726.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(4967..5842)
                     /locus_tag="IV038_002564"
     CDS             complement(4967..5842)
                     /locus_tag="IV038_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342727.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(6059..6316)
                     /locus_tag="IV038_002565"
     CDS             complement(6059..6316)
                     /locus_tag="IV038_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342728.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6319..6543)
                     /locus_tag="IV038_002566"
     CDS             complement(6319..6543)
                     /locus_tag="IV038_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342729.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(6716..7759)
                     /locus_tag="IV038_002567"
     CDS             complement(6716..7759)
                     /locus_tag="IV038_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP8342730.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2196 a         1626 c          991 g         3050 t
ORIGIN      
        1 ctgtatatag aaaaatcgca tcacacgtat tcaaattttt aagtatcttt ccattatttt
       61 cgcttacatc aaacatttta atatttggta atagttcagt taatttattt tgaaaattta
      121 catgtcctcc cactaaaaga atgtttttat gttttagata agctatcatt tcagacagtg
      181 aaggctcttc agttttacca tttaatgatt gtctatgttc ttgctcctgc aattgcttta
      241 atactttatt ttcaaacctc agctgttcaa gttcttttct gattcctttt aattctttaa
      301 tctcttgctt caaaagttcc tcttcttttt ttgccgattt gactaaagat tctaaccgac
      361 cgttttcttt actcgcaata cttaattctt ttcttaattt gattacttca gatttgtttt
      421 cttttctaat acttgcaatt tgttttgcta tttcataatc agaattcagg atcaattctt
      481 ccttagcatt tttaaacaac cgagttaaaa cgaactgata taacaaacta taaaaaacta
      541 gcaaatagtc aatttgtcgg tactcggtat tctgatcaat tgcatataga gagattaaaa
      601 cgaaatacac aatatcattc agctcgctac ccttgatctc aaaattattg actaagtaac
      661 tatcaatacc catcaaatct gcaaaatctc catacaaaga aaaatatgat tttgacaata
      721 tatctatatc tgaatcgtca aacccattta ctattttccc atcattgtta gtagttgcta
      781 acgaagcaat agtgactttt gaattgggtt ttacaaatcc tatacctaat agttctttca
      841 tctttttaat ttcaagtttg ttatcctttt gttcggcttt atgctgtttc atattgtaaa
      901 gcggataaaa tgctgttccg taagcacctt ttagtacgtt atctataaaa taatcttcag
      961 aacctgtttt gaatagaaag ttaatcgcag ccaaattaga taacagctct tcagtgttgt
     1021 gcatcaattt tttatcattt aataaattat ccccaagata tttcattgca taataagggt
     1081 ttgtttcgct acttattttt aatatttttt gatagctatt cctcacgata ttaaccaaaa
     1141 tatctgtctg attaattgaa ttacagtaca aagccactcc taatgccata taaacattca
     1201 aatcatgttt tgccgtaccg ttgaataaaa cgtcttcaac ttttgaatta tctcgtctat
     1261 actttttgat catatttttg atattttgat cattctcttg gtatattttt ttcactcgtt
     1321 ccaatgcctt gggtttgttg gaaacagcaa cagcgaagtc tagttctttg aaatcaatat
     1381 ttagtatctg tacctcatca cctagattat aatctctatt tacgctcgga aaactgctga
     1441 aatctaactg ttttttacct atttttggcg aaaatattcc ttcgatttct ggcaaatgta
     1501 atcacactct ctccttattt atctttagaa aatttgtcac tattctcctt atatttatca
     1561 ataaaccttt ttaatcgagc aatataagcg ttaacccttt taggttcttc ttttcgccat
     1621 tcatcgttta cgtgaccatt ttcaaagaaa gaagctaaaa gagtttccac ttcattaacc
     1681 atatctgcga tcgtatattt ttctgtaccc tcactcaatt attcatgaac tgttaagtgt
     1741 tcatagctaa gttcattcat aattttgtta tagcgttttc ttgctttttc aaattctttc
     1801 tgttcgtcaa attccacttg attcttcatg ttgttcttcc tctctgtttg atattttttc
     1861 acttcttcaa gtttagcatc tagttccgct ttaacttctg tcacttcttt aagcgcttgg
     1921 gataacttat gagacaattt cgcataagtt tctctcttgt attcatttcc tactcccaac
     1981 tcttcggagt agaactcttc tagtagtctt gcaaaattaa catttctcct gccagcatag
     2041 ttttttagct tctcaaaatc agattgaaag acagcaacac ttttgtaatc cttttcaccc
     2101 tcaaaatcaa aagcatagtc atactttcct gaaatcaatt catcaataac tgtagcaata
     2161 tattgagaga tagaaatatt atcttcactt ctatgtcgct ctataagatt atatatatca
     2221 gggctgacat gaaacgtact tctctttttt gtttgccttt ttctaactgt tacgaccact
     2281 tttggaactt cactatctcc attgatctct ttgtattttt caataaataa ttttctagct
     2341 tgttctcgac tttttgccgg aatttcatct acaattttat cttctaaata aatatagtgt
     2401 gttctcatac attcgtcctc tatcttcttt actctcaatc ttcatgatct tagttcttac
     2461 cttgtttact cttaaacatt gcaaaaaaat ctagtcaaaa tcaaacttga aatcatcgtc
     2521 tatcacaaca cccttaactt catctttaga tttcccaaag gaacccttat tttcaaatcc
     2581 tttatttttg aatgatgatg gttatggaaa acttcctttg aaattctgac aagcataact
     2641 agtcagaact gttttactta gattaagctt tcaaaagata ctgatgaaga actaagttct
     2701 cagtaccttc ccattcttca ataaaaaata tttctccttc ttttacccac tctatagcta
     2761 atttcttata atcaaataaa ccatatgtgt ttaagccttt gtctttcatt aaactcaaaa
     2821 actgatcccc ttttaatctt tctgtatcat ccatcagaaa ttcaactaaa tctttatcca
     2881 tgcgtgcaac tttttcgtct tttagtgcca ttgatttagc atagtcagtt gaatctttta
     2941 gaataataac acctacattt ccttccacaa ttttcttctt catatttacc tcctaccatc
     3001 ttttccaaag agtatttagt caaaatcaaa ctcgaaatca tcgtctatca caacaccctt
     3061 aacttcatct ttagatttcc caaaggagcc cttattttca aatcctttgt ttttaaattc
     3121 taaagctttt tcgacttctt ctttggatat tgtatgaact tttttcttaa ctactccaag
     3181 tttatacagc tcttcaaact ctggtacttc ggatctgaaa tacaatccat cagtacgaat
     3241 ttttaatagc ccatatgcgc tatgaacgta tttctgttca tatctaacca tacgatctct
     3301 aaatacgcct gctaccatgg aggaatacgc tcgattatac atactgtatt tatcgaaggt
     3361 gcttgtttct ccttctctta atgatttttg tccgatagca ccaaatactt tcgttctaag
     3421 atttttataa agcttcaacg tatcaggatc agtttttaat ggctcgattc tttcaaaata
     3481 ttctttgagt aatccttttt tcgtcaaaaa tgtgtggcta tctaggactt ccatctcttt
     3541 aatatcataa taatcaagga taagtgttag ctcaatctcc gataaccaaa taggctcatt
     3601 agctgttttg ggattttcgc cataatccgc aaacatcgac tgtgattttg gattagcata
     3661 tgttccttct ttggctgttc cttcaaaatt aaccaaaaag aacatatacg tttttaaatt
     3721 ttcatacggt cttacttttg gtctatttat cgctctcaaa atagcttgat ctgtattata
     3781 ttttgctttc aaataagtgt ttgacggcaa aggataattt ctcatgatcc tcgaataggc
     3841 ttcgttgaaa tcatattcga tcatctttgc tccttcctca aacaaatcca agttatacat
     3901 gctaccggag gtactggctc tataggctga acttggaaca aagaaaggtg catcttccgg
     3961 cttatcatag cccaaaaaat gtattcctgt ttgcttgttc tgacggtaaa tgtccatcaa
     4021 aagatatttg atcgtactaa aagaatctaa ttttcctctt gtcatccacc ctaatccgac
     4081 aagtctttta attttattct gcatttcagg tgtgtatatg ctattgtttc ccatttatct
     4141 gctcatctcc ggctttttta ttctagaccc tatatttgtt cattacacca cttatctcgt
     4201 ctacaaaggc tcccagatcg tgtgtaaagg catcatagta atcttcaatc tcactcgatt
     4261 cagttctccg gtttcttccg tttttatttc tgaatagata catctgcgtt tctcttaaat
     4321 cccttgcttt tttcgcaagt gctaataaat ccgtaaaatt atcaataacc ataatgtcat
     4381 tgatcataaa accttcttca tgaccatact tttcagccat ctcgataaaa tcattaacat
     4441 cgaacaactc caataattct gctttatatt tctctaacaa tgtatttaaa tagcgatttc
     4501 tcaattccca tgaactttca ccagttccgt attcaacctt gatccctaaa ttcttagtgt
     4561 agccttttgc tgtgtatcgt ccaatgaagt ctcccttatt ccgattgcta tcttcttctt
     4621 tgaaaaactc acgccaaatc ccctttttct tcttaggaat tagacctctg ctcttgaaat
     4681 catcatattt ttttgaagct tctcttcgtg tatctaagta cctttgcttc agctctgtag
     4741 ctgattcatt cgttccatat tcaacagtga tccttaagcg cctgctataa ccttttgctg
     4801 tatatcgtcc aacaaagtca ccgccacttc gatcactact gacttcctta acaaattctc
     4861 tccatatacc ttttttgtcc tttggtatta atcctcgtct tttcatatct tgatattttt
     4921 cagaagcttc ttttagagta ttcttttttg ctaccatata ttccactcac tctttgttat
     4981 ctagtatttt aataactggt ttcatttttg taatatcttc tttttcagct atatacaact
     5041 ccgttacggt aacgatctta gtatgattta gattaattac tttcacttga tcgccaattt
     5101 tgatttttcc tcgcaaccgt ttgggaataa accagttgta taatcgtgat ttattattat
     5161 ctacataagc ttttattgca ttttcaggaa acaggttatt tagtctttca atttttttac
     5221 ttttaaaaaa ttcattcgca aacttttcat caacaactaa attaaataaa gtttcagaat
     5281 ttacagcttc catatctatt cctttgtaga taggagaaag caatttttta tattttgcca
     5341 gtaatgactt tccagaatta ctaatcttaa tttggttact taaatcttct tgataaagaa
     5401 ccccatatcg aagtaacgcc ttaatataat gttttaatat gccgttgata ccagaataaa
     5461 tatccatgcc gttctcaata tagtagaaag tgtcgtctct ccaaaacaat ttgaagctat
     5521 tattcaaata aagtaacaag atcaatatat gtttgtcaga aattctgaca aatctattac
     5581 tttttagata ctcaccaatg gcttcactta actctttgaa gccattgtat tttcccgata
     5641 aaaatgtcgt aacattatct tctctctttt tcctatggtg tgaacttacc ctgaaataat
     5701 agagtctatt ctcatttaat attgaaaaat aaatactttc agttgtttgt gagcattgta
     5761 tatagttgaa atgatatcca tctggcatga cttttttaac tgtattttgt actagctctg
     5821 accacctttt gttagtagtc atatattttt ttccattaaa ccaaacataa atatcttttt
     5881 taaaaatttt gttccccccc tattgcgttt tctttgacat taaagccctt tgttttcatc
     5941 acattcgtat agccctagcc actcataacc tgatgctttt gcgaagttta tttaaaagtg
     6001 ttttcctttg taatctacct ttcccctcgt cctggtactt ttcttggcga ctattaaact
     6061 attcaacttt tttagaatca ttgtcagctt gaaatccttc acgtttcatt atgtcttctg
     6121 cttttccaat gctgattcct ttccaatcac gccatcctac gcttccctta ttttgacgat
     6181 aggctttaat agctgattcg gatatttgcc atcttgtagc caactctttg gctgtgtact
     6241 tatttgaaaa aagtagcttc tctatcagtg aaatatcaat cattttattc tcactcctga
     6301 aatcttcatt aatcatctct agctagctat atcttcaatc aattctaata tgtcggataa
     6361 accatcaaca gcttcttcaa gttgatcaac ggattcttct gctttttcat atctttctga
     6421 tccctgtaga tttttaggta tattttcttt gtactcttct tcttgaatta cttctaactc
     6481 atcctttagc tcggaaagtc gctcgcttat ttcttctaac ctttttcttc ttctttggtt
     6541 catcaaaatc acctcttcgc ataatctcta cgattttaat ataccacttt tgtgtactac
     6601 attcaatatt ttttgttata gattcgtatt acaaaaattt cctctaaaaa aataagagcc
     6661 tatttaccat ttcagtaatt aagctcttat ttcatttagt tgataattct atcaattaac
     6721 gtacagcaac cgccattcca gtcacattaa atgctctctc atacgtgctt gcattgatcc
     6781 attttgcgcc aaatacaggg tcaacaacat ggtatgcacc atttctgaag ccatccacga
     6841 gaactacatg tccattccat actgcacgac cccaaggcat ttgtttgaac tctggcgtag
     6901 catagttata agtaatccaa gttacaaccg gattaccatt gcgaatttca ttcttgattc
     6961 cattcgttcc tgaaccagtg atattgacgg cattaccgcc aacgtttttt gcccatttaa
     7021 ctactgggtc agccatcatg ccttgatacg ttccattaac gttatgacgc cactctcctg
     7081 agtaaccatg ataaggatta ttgtcattag cttttggcat atagtttaca aaactggtta
     7141 agctttgatt tgtggctaca cctttgtagt gtaaaccttc taataaagaa gctccttcgc
     7201 ataacattgt atttccttgc catacaacag gaacgttcaa cactttttca gtgcttggag
     7261 caggtgtagg atttggtacg ctgtttggca ttgcatacca gcctgtacct tcaatcttcc
     7321 aaccttgttt actaattacg ttcttttcat tttgatctaa cgtgtagtga tgccaattca
     7381 cgtatttgtt atataaacgt gttacaggaa ctctatttgc tccgttgtag gaatagaacg
     7441 caacaccttc gtacgtccat cctgtgtgtg aaatcttatc tctttctccg gcatcaactg
     7501 tgtaaaaatg ttcaccgtta taccgattat acaaacgata aacaggagta ccacttgttg
     7561 gagcttgcca tgcgacacct tcgtaatgcc atccataacg tttcagattg tctctttctc
     7621 caacgcttct agtgtataga tgctctccgc tattgggatt atacatacga tacatggaaa
     7681 tagagctttc tgctgaaact gaaggtgata ataatactag ccccattgtg aatagaattc
     7741 ctactaaaaa ttttttcatt tgattttacc ccttttcatt ttttattgtc attattgcat
     7801 gaaaattatt cgtaagattg ccaattacat aaacgattgt tttaagatca taatcgttgt
     7861 tgt
//
LOCUS       DACDCJ010000106         7654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDCJ010000106 DACDCJ010000000
VERSION     DACDCJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.107"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(41..733)
                     /locus_tag="IV038_002568"
     CDS             complement(41..733)
                     /locus_tag="IV038_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8342731.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            818..994
                     /locus_tag="IV038_002569"
                     /pseudo
     CDS             818..994
                     /locus_tag="IV038_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1170..1739)
                     /locus_tag="IV038_002570"
     CDS             complement(1170..1739)
                     /locus_tag="IV038_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8342732.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1813..2136)
                     /locus_tag="IV038_002571"
     CDS             complement(1813..2136)
                     /locus_tag="IV038_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342733.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2290..3102)
                     /locus_tag="IV038_002572"
     CDS             complement(2290..3102)
                     /locus_tag="IV038_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342734.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3456..4304
                     /locus_tag="IV038_002573"
     CDS             3456..4304
                     /locus_tag="IV038_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP8342735.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4449..5390
                     /locus_tag="IV038_002574"
     CDS             4449..5390
                     /locus_tag="IV038_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP8342736.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5380..7551
                     /locus_tag="IV038_002575"
                     /pseudo
     CDS             5380..7551
                     /locus_tag="IV038_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2278 a         1516 c         1378 g         2482 t
ORIGIN      
        1 gttcaacttt tggggttcag ttcaaagagg gggggatttt ttatcgttgt ggaatattta
       61 aataccaacg cttgtcatga aaatcttgtg ctccaccctt agtatttcct tctggatcgt
      121 ttgtcgcacg catcatgacg tatactttct tattagggaa atcacgcata ttgaaagata
      181 catgatagcc aacatttcct aaagtattat aagcttgatt tacgtctggt ctataaatcc
      241 catcagctct tactctagct agttcttttc cagtattgta gtccataatg aaaatatact
      301 cgtatttata attagcaatg tgccatccag ctacatgcaa gtttgcgttt tcgatttctc
      361 cgaactgatc aatgtgggcg taatttgttc catctgtcag cgtagaattt gcagcacctg
      421 ctcgagttgg atcaattact ggtttatcct ccgaagtagt tggattatca tccgtaaaac
      481 catgagccaa atcataggct agtttttctt tacttacacc tatttcagaa agataaccgt
      541 aaggatctgt atgatcgccc caaatatttt gtgttaccca taaatgcgat ttgattcctg
      601 gttggttata aggagtgtct aatgttaatg gaataccata tttcattgct gaatctcttg
      661 ccaattcaac gtatacttta tagttctttt caaaagtttc tttatcatgt gtgtgttgta
      721 actcgatttg cacaggactt ttggctcacg aggttgtcct actctacatc ttgctgatag
      781 atatgctgaa aggaaaacac caacattatg ttttgctttt tcagctagaa cgagacaagc
      841 aggaatacct ttagaagata tcaaaggttt tttaggtcat aaagatgttt ccactacaca
      901 agtctatgcc catatttctc ccgaagtgaa aaaaagatca atgaatcaac tagaaaacta
      961 tatagaagag caaataaaaa agcactcaaa ttgagtgctt ttttaagctc actatcacta
     1021 aaactatcac taattttatt tataagcgaa catgtttcta tcactgattt tttaaatcta
     1081 gcgaaaaacc cttatcttaa aggcgttcgt tcaattcttt ggcaaggtct tcaaagcctg
     1141 gcttgcctag aagtgcgaac atggttttct tatagctcat gtagaaaaat tttcttgatg
     1201 atgattttat ccatcaattg agattgactg ttttgctctc ctatgatttc agctatgatt
     1261 gtccgatttt gcggttttct tcttaactgg tagttgattt tcccagtttc catcaactcg
     1321 ctaagcatat ccacgactcc gccaaatccg gtctttttcg taataaaacc tagtgctacc
     1381 attcgttcat ctagattttt tgctgtaatc gtcacaccag taatgagcga gtgctgtaat
     1441 gtttgggcca gctcttccat cccttcagca ttggatacga cggaagctgt tttgttaata
     1501 ggttcttcgt ttatttcttt tggttctttc ctctccactt tcactgtttg acgaaggaca
     1561 tctagttctt gcaagagcgt ttcgtattgt tcttgggtca gcatcacgcc agctactttt
     1621 tcacgattaa atatatatac gccagttccc gcgtctcgcg cttgagcaaa tacatccatc
     1681 ggcgatcgtt tcacttctgt gatcgaagat gtgggaactt ctaatttctt taattccaat
     1741 ggatttcctc ctttactaat cgctttttat tatagcacag actgacttgt tttgatcttt
     1801 tttccaaagg ttttatcggt ttttgcctaa ccagtaattg atatcggtaa gaagtctttc
     1861 gataccaaat gaaggatggt acagatacgt tttttgatct tcgttatggt ctagtggaaa
     1921 aaaatgatcg atgatcacta gatctgcata ccgaggcggc ttatttcgag tacatacatg
     1981 tatagagatc cgttttcgat gtcttagcgt tttgttcagt accccagcaa gtcttcttgc
     2041 ttcttggtgt tcatagcgaa aaaaaaagac gattttcact tcaggagggt ggacatctaa
     2101 ataccctcgt tcctggagtg ttttcagtaa aagcatcccc cactctactg cggggtgtgc
     2161 aagtgcttgt tggatcgcta gccgaaattg atatagttta tctttggaat tgggaaattt
     2221 ttctaatgtt tcctgataat aacgaacttc ttctatcagc cggtcatgga taccaattac
     2281 gtaacgaagt cattcttggt aaagtacttt tgtcagcttt gatttgcttt cgctctcaaa
     2341 ttcgctattc agccaaaaca gcgaatcaag cttttcagtc acaatcgtca atttccgata
     2401 gtcttgtgtc tcttctatcc gtttccaccg cttttcagta atctggttac agctcaagcg
     2461 caaaattgct agataatttt tttcttttat tggcaaaatc tgctcctctc taaatagcaa
     2521 ttcaggaaac aattcaaaga atagtgttcg actgtctcct aaaatctcga tttctgccgg
     2581 atcctttctt tgcttcaata attggatgaa catctcagaa aaggactgct ctccatcatc
     2641 tgcaaaaaaa tgcggaaaat ctttcaaaaa gcaaaacagc agatatctta cttttctttc
     2701 tgaaccaaag atctgtgctt ttttataaga aaaagaaagg tggtactcgc caaaccactt
     2761 tctcaatcgc tcttgcgtag aacgatcgta cttctgctcg taaggttcat tcaatagcag
     2821 atttttcagt aaacaaaacg ggggtgattg gatcagaaaa acaaacaaga gataaggttc
     2881 gctatatcct tgatcataaa ctaactgaac ggttttctta tgaagaatca gctgaatggg
     2941 acagtcgctt tccgccaaac gtttcagatg ccgatccact gttttaacat cgattttcag
     3001 ttcttcagct aaatcagctt ttcgccagat caaatctttc attagattga ttagtgaaaa
     3061 taaattctgt gcattttttg ataatagttt ttgccatctc atcgatcatt tctcacctga
     3121 tttctaaaca tattgtttga tcaaaatggg ttcactccta aaaagataaa aaactccctt
     3181 tctatttatt taaatacgca aatcttcctc attatttttt attttactca atttaatcgt
     3241 attctcattt caaaacgaat gtactttaaa aaaacataat aagatcactt tgcttgtagg
     3301 atgtattccc ttccattaca atgaaataaa tagcgatttt atgagaaggt gatgtgaaaa
     3361 tggaagaaaa aatagaagaa tccaaacaaa aaatcgatca gttgatataa gaattagaaa
     3421 aagcggtcat cggcaaacgt tcaactatcc aattgatgat cgtggcccta ttagcaggtg
     3481 gtcatgtctt attcgaagat attcccggtg taggtaaaac gttactggtc aaagcactag
     3541 caaaagctgt acagggaacc ttcagcagag tccaatgtac tcctgatctg ctgcctagtg
     3601 atattcttgg tttttccgta tacaattcac aatcaaaaga gtttgaattt cgtccgggac
     3661 cgatctttac cacgatcttg ttagcagatg agatcaatcg tacgactcct cgtactcagt
     3721 cagcactttt agaagcaatg gccgagaatc atgcaacgat cgacaacaat acataccctc
     3781 tagacaatca tttttttgtc ttagctacgc aaaatccaat cgagtacgaa ggaacatatc
     3841 ctttgccgga agcacagctc gatcgttttt tattccgact acaaatcggt tatccttctt
     3901 ttgatgacga acttttgcta cttttggata aattcgaaaa agtgttggat aacttaaatg
     3961 aagtattgaa cagttatgaa atagaagaaa taaaaacaaa tgtggcgaat gtctttgtca
     4021 gtcccgaagt ggcatcttat gccttgcagt tagtgtcagc aacaagaagt catgctgcca
     4081 tccagcttgg catcagtcca cgaggaagcc tttcctttat tcaggcagca aaagcttatg
     4141 ctttaatcca tgggagaaat tacgtcacgc ccaaagattt gcaggattta gttccttacg
     4201 tgttttccca tcgcctgatt ttttacgatc gttcattgaa ggaagacgaa aagaaacaaa
     4261 tcattcatac tcttgttgca caaattccta ttcctgtgag gtagattgag gtagacaatg
     4321 gaaaaaatca aagattattt cattaatttt gtgttatttc tggtatattt catcattgtg
     4381 ctttttgctc ttgtattcaa caattcttta ggctggttct tgttggcctt ttttactctt
     4441 tatgtatcat ggagcttgat ttcattaagc agctttctaa agaaactttc ggttacttgt
     4501 acattgcagc cactcgttta tcgcaaaaaa agtatgcagg ttattttttc catagcaaaa
     4561 aagagaactt tttctttacc tctccctcgt ttacaaatcg gcttcccagc aatctttgca
     4621 gaaaaaaaca agagattttg attcttacaa ataaaccgca agaagtccaa ttgctttggc
     4681 agccaaaaga aagaggatgc catgaaacat taactgtcat ttttacagcc tatgatagtc
     4741 taggactttt cagaaaaagt gcaaaaagag agatctcttt tcctgtgact gttctgcctg
     4801 ctttttgtaa gattcaagca gaaaaattac agagagttct ggaaaaaaaa cagcagctga
     4861 acgcgtacca aagaggtctt gattttcggg aacatcgcgc ttatcgtcca ggtgattctt
     4921 tcaaccgaat cgattggaaa ttgtctgctc atactcaaga gtggttatac cgtgaatacg
     4981 attatcaaga agaagaatgt tctcctcttt tttgcttttg gggttcgccc agtccaaaat
     5041 ttgaacctct gctagacttg tatttttctt tatggcattt gagaaaagaa aagcagccgg
     5101 aacttctgat attaggcaac cgcgcctttc atggcaaaga ccccggtcat acctattttg
     5161 cttctttgga agctggcgat gagaaaactt ttttcgcaca cttgaaaaaa tatgcaaaaa
     5221 aacaaatcgt cctctttgtt cctgagaatt ctcctgaagt ctcagaagct attgaaacat
     5281 tgtcaaaaga tcgaacggtt tatcttgttt atttcgtaga aaaacaactc atggtccaag
     5341 aaaaagacaa gatatgttca ttgccaggag gtgaatggat tgacgattaa acgggaaaaa
     5401 tggctgcgtg gcgtttactc ctttgtgata ctaagtgtag gtgctccgat atttgtagta
     5461 gctaatcaat tgcaaaacag ctaccttctt atcctaacta ttggtgtcct atgccttatt
     5521 gtctgctgta ttcctaaaaa atgggtacgt ttgctgcttt ctttgccagt aatgataggt
     5581 tgtttgtttt tatattttcc ttcgaagaca ttttccatat tatggatctt acagtttttt
     5641 tcgtcagtta cagaggaatt cttccatcta tcccaaggta aactttatta tttgccagaa
     5701 aagacagctt tttttctcat aatgttgctg atctatttat ttattacatt gaccgtggat
     5761 tacgactatt ggtacgcacc ctttctttct ttgatggctt acttcatgat gcttaccgtt
     5821 tttcggaaaa aagatttact gtacgaaatg attgcggtag taactgtttt gttcgtgtat
     5881 ctggcagcaa ggcaattttt ctccatccgg ttgttgtcga gaaacactcg attccagtca
     5941 ttgactacag ttctgcttat ttgtttctta acttttagcg ctatcttgcc tttatatctt
     6001 ccgaaagtac accaatctct cgaggaaacg agtgaaccga tccgagaata tcttaatgat
     6061 gaagggcttt atgacacact ccatgagtat caattaaccg gaagtgcaag aacaggcttc
     6121 agtgagaatg atgaacagct tggtggacct ttatatgata atggcgaagt tgtttttaca
     6181 gcgaaccaaa aaggaaatgg ctattggaag atcgaaaata aaaatcatta caccggttct
     6241 ggatgaatag aagatcagga cgcacccatc caaccaatcc aagaattccc tgttgacatc
     6301 acgagctacc aaaccgttat agaagacaaa gaaaacatta cattgaccct aaaaaaacaa
     6361 gctttcttac cttatcctta tggaaatact accttgactg atttttcatt agctagtgga
     6421 caaacgcttt actatttgcc tgaaagccag cgattcattc ctgtcccgaa tgaatcgaca
     6481 cgttctgcaa ctttacaaac agaaaggtta gtttttacga gagaagaatt aaaaagcagt
     6541 cagttaaaag ctgatgattt agaaaatcag cagacaaatc ttcaattgcc aaaaagtttg
     6601 cctaaacgta ttgaggactt ggcggtcgaa ttgaccaaaa atgaagcaac tttaattcgt
     6661 aaagtcgaag ctgtctaaca gtatttaaaa acaagcggaa atttccgcta ttccaaaacg
     6721 gataccgggc atactccaaa agaacacgat tatgtggatt atttcctctt tgattcaaaa
     6781 gtcggctatt gtgacaactt ttcaacttct atggtcgtcc ttcttcgaac acttggtatc
     6841 ccagctcgct ggacaaaagg ttttacaaat gggctcagaa caggcacaga taatcaaggg
     6901 aatgaggaat acagcattct caatagtcat gctcattctt ggccagaggt atactttcct
     6961 ggatttggct ggcttccatt tgaaccaacc ccttcatttg ccaatccaat gggttcacaa
     7021 ccagttgctg aaacaaccga aaccgtccag cctgctatcg aacaaccaaa tgaaagcgaa
     7081 aatatggcag aaactatttc cagtgagaga tctgaggtca ttccatcaga agaaacaaca
     7141 ctcacaccag cttataaaat cgtcttattc agccttggag tgtgtttatc cgtcttgttg
     7201 ttctttctat tgttattcag aaaatctctg cgctggacaa gttatcttat ctatcttcat
     7261 ttttttgctt ctactaatac gattgcatat caaaaacttc ttaacccttt ggaaacattg
     7321 gtaccgcgca aacccgcaga atctttgcag gaatatagcc tgcgaataga acaagaattc
     7381 aagctagatc tgcgtttcat tcgattaact actcaatatg aagcagaaat atatggacga
     7441 caagcagatc cggtaaaatt cgataaaaaa acctatttag aaataacgca tataataaga
     7501 aaaacggtat acaaaaaaat tctgacaaaa gggagccgct tcaaaaaata agataaaatt
     7561 tctggagaat ctacttctcg aatgccgaga accagctgat tgagtatcga ttactcgaaa
     7621 aaaaggttgt gacacgtttt ttgtcacaac cttt
//
LOCUS       DACDCJ010000107         7609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDCJ010000107 DACDCJ010000000
VERSION     DACDCJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.108"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            905..1156
                     /locus_tag="IV038_002576"
     CDS             905..1156
                     /locus_tag="IV038_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342737.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            1239..1511
                     /locus_tag="IV038_002577"
     CDS             1239..1511
                     /locus_tag="IV038_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342738.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            1601..1840
                     /locus_tag="IV038_002578"
     CDS             1601..1840
                     /locus_tag="IV038_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342739.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            1987..6270
                     /locus_tag="IV038_002579"
     CDS             1987..6270
                     /locus_tag="IV038_002579"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP8342740.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            6369..6734
                     /locus_tag="IV038_002580"
     CDS             6369..6734
                     /locus_tag="IV038_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342741.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            6736..7188
                     /locus_tag="IV038_002581"
     CDS             6736..7188
                     /locus_tag="IV038_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342742.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
BASE COUNT         2689 a         1171 c         1649 g         2100 t
ORIGIN      
        1 aaaagggttt gttgaaagtt gctttactgg cagctttttt ctaagtaact ttttgacaag
       61 ctcaatggca acacaagcgg aagaagtaat acaagaacta actgttatga ataacagcta
      121 gataaaatgg gctatgtatg ttatccaaca atttagtaat cactagaagc ctagcgaaag
      181 ttcgcttgat aagttataga tgagtattga tttgtagtgt ttccaaatca aaagggtaca
      241 gagccacatg aaatatgttg tcctctgcag cgtcaggatg ggaatccata ccatggagat
      301 tcggagatct gtgagggaat acttacgtgg ttaatatgtc agttgtgttg gacgtagtaa
      361 tggtcatagt gggtagattg actgaattct tatatgagaa aatgaaacca acaagaagat
      421 agggttattt atacagaatt ggggatcttt tctggaaaag taatcggata atgcgagatt
      481 cgtacggtct tttttaaatt tctctaaaaa aaacggcatc gaatgggttg ggtgattgat
      541 aagcaatgaa tcatagactg atatagaata aaaaatgatt ctatttatct ttttacatct
      601 atcgtttttt caagtagccg caaattatat cagcattcaa caatttttgt atacaagttc
      661 tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga taaaaaaaga atcaggtttt
      721 actgagtttt tcttcaaaat ttgtgttatt aggttaggac aaagataaga agaagtatga
      781 agatttaaaa aaggaatgag gaaaagatag taatttccga ttattggagt ttttttattt
      841 ataaagaaga agcgaataag ttgtgattcc tagatttcgc gcgtgattaa ttaatgagca
      901 aaaaatgcgg aaagttttag tgtttaatac acgaggacaa agtttcttag gtattgaagg
      961 cacacgtgta acggttgatg ggtggtgtga ttgtttcgag gttggaaaac atgttgaact
     1021 tatggcattt actgagctaa aagataagaa caacgtggaa tttttcgaga gtgatattgg
     1081 ttgggacgat catgaagaag tttatggaaa atttattttg cggaagggaa attcttatac
     1141 gaatggggaa gcataagtag cgatttgtta gagatcattg ataggattga aattgtcggg
     1201 aatatttatg agaatctaga gttatttgag gtgatagaat gagtgatggt tttgaacaag
     1261 tatcaataat taaaaatcaa gttcgggaga ttcttagaaa aaaaggctat ttggtagata
     1321 gttattttga aggagattac gaaacatggg taggtgtcta tgctcgtcca gagaataaac
     1381 ctacctattt agatcctacg acgtcagaag atgcctattt acaaaatagg tatcgagtag
     1441 atggttttaa gcaagacttt gcggagtggt ttgaatggga aattgaaaac ggagaagtaa
     1501 aagaagaata agagtacttg ttgtatcaaa ataagccagt agaacaacca aaccagtagc
     1561 tagtaatgac aataatactg aggaatcgga tcattatggt atgattgata tttctcttcc
     1621 agaagatctt aagccagatc ctgtaacaat tggcaatagt gtcatgattt ttgattcatt
     1681 agatgaagct atgtcatatg gttggaaaga aattaaggat gaaggtagta attggtatgg
     1741 catgagattt ggtgatggtg aagttgccaa aaaagctggt attccaattg ggaaatggac
     1801 agttgatttc cacgaagcag gttctttttt tgttctgtaa ttatagtggg agggtaaaat
     1861 cattgatgaa atgtgttcag ataagagcgc cagataaaaa tagaagagac agttcacgtc
     1921 gttatcactt ttggtaacgg cgttttgttt tattatataa aattctcagg aaaaggagga
     1981 attcttgtgt atgcaaaaac gaaaatgaat tggttgaaag cactcgcatt ggtggctttt
     2041 tctcaattct gtttcttggt gggaactgct caagcacatg cagaagaaac gacggctata
     2101 caacctacga tggaagccat tgcccaagtc gcttctgaag tgacagaagt ccaaccagca
     2161 gaagccgggg tcccagctgg tgaagccact gttgaacagg cccctgtgac agaagcgcca
     2221 acggtcaatt ccaaaacgac tctcgatcag acgattgggg aaacacaaca cccagggaat
     2281 agtcctggta cttcacaacc agttgaagaa actgcctcaa ttacaacccc tgcttcagaa
     2341 acgtccactc tagaaggaga aaaagctgcc gaagcaacgc cttctgtgca gccagagtct
     2401 tctgagaaac aagcggaaga aaagaaacga atagaagaaa cagtggaaca accagcggct
     2461 actgctgaca aagaaggaaa aaatgaggaa aacgttgaaa aaacaatctc ttcaaccgca
     2521 gctagtcaaa atcaaccaac gaaaccaaac aaagaatcta cggctagtat tgaaaatcaa
     2581 aagaaaaaag agcagaacat tccaaaaaca ggtacaccta cactgttagt aacagtacca
     2641 accaatcgtt cggttcgtgt tcgccgtgca accaatgcag ctgttttagg tggtcggaaa
     2701 attgattggg gcgaaaatat tggtgttact tctagtccga acaatctaaa cttcacttta
     2761 aattatatgg ttcgttttga gattaatgga caagtggcgt tttgtattga gcctggtgtc
     2821 tcagccgcag aaggtcaagt ttatacagct actgctttga aaaactattt gaaaaattca
     2881 aatgtacgga aaaaaacaag cttgatctct tatttaggat atgtcacaaa taaagacaaa
     2941 tccgatgaac aatacattgc ggcacaattt atgatttggg aagctttggg cacaaaaatc
     3001 ccaatcaaaa atatccgtgt tgattatgat cgtcgaaaag cagaaattca acgtttgatt
     3061 aataactttg gtgaaaaagc aacttttaat aatcaaacac atacggtaaa aattggcgaa
     3121 acgttgaaag taaagaataa aaatgatttt ttgaaatatg tgaaagacat tcaaaaacca
     3181 aaaggtgtca atgtatctat tcacggcaat gaagtatgga ttacagtcac aaaagaggca
     3241 ccggataaag caaaaattca attgaataaa attaaagttc aaggtgcgcc attagcctat
     3301 cgtcgtccag gagctcaaac aattggtgtg ttacatccaa cagaacctaa tgcgtctgtt
     3361 attaatttac aaattctaaa acaaggaaac attcgtgtgt ttaaagaaga tgcggaaact
     3421 ggtgtgaaag cacaaggtgc agcttcttta gaaaatgcgg tctatggttt gtatcaagca
     3481 gatgggaaaa agattaaaga aattaccttg aaaaaaatta acggcaaagt acaggcagaa
     3541 attaaagatc ttgatcctgg caaatatgtt ttaaaagaaa tcaaagcgcc aaaaggttat
     3601 gtgctttctg atgaagcaat tgaaattgtt gttgaacctg gaaaaactgt caatgcgacc
     3661 gcaaaagatc aagtgatcaa aggaaccatt gatttagtga aagttgctaa taaagattta
     3721 gtcgattcaa cgaatccaga taacaaacca aaattagctg gcattgaaat cactttgacg
     3781 tcaaaaacaa ctggcaaagt ggtgaaaacc gttgtgacag acaatgatgg ttatgctact
     3841 tttggtaaaa ataccgttgt ctttgatacg tacattcttt ctgaaaccaa aggaaaagaa
     3901 gggtataaac tatttgaacc acttgaagtc acaatttctg aaaatgggca aactttccat
     3961 tatgttttag aagataaagt aattgaacaa cgtcttaaag tggtgaaagt ggatcaagaa
     4021 acaggaaaag tgattccact tgctaataca gaattcaaga tttttgatac tttagccaat
     4081 cgttatgtca caatggagat tccaaatgat acagaaacaa cggatatctt taaaaccaat
     4141 gaaaaaggct ttttcctaac taacggtaca cttacttatg gaaaagatcg ctatcgtttg
     4201 gaagaaatca aagcccccgt aaattacgtt ttgaatcaaa acccattagt gttctcgatt
     4261 accaatgata cagaaccgat caaagtgatc cattttgcga accgtcaagc acggaaaagt
     4321 gtaaaattat tcaagtatga agaatggaac aagaagaaaa cgccgctcgc aggtgtgaac
     4381 tttaccttgt ataacggtaa aaatcaagca attggtgaat ataccactga tcaaaacggg
     4441 gaaattttag tgaaaggtct agttgccgat gactattact tcttggaaaa tgccccatta
     4501 gaaagtttcc aaccaaatac agataaacaa gcattcacgg ttgcattcaa acaaaatgcg
     4561 ccagatgatg gggaaacgtt agtggtttct gtacgtaatt acttgattcc accaaaacta
     4621 gaaacacaag cgtcaaataa agccgatggt gaaaaagtgg ttgatccatt agaaacgatc
     4681 gtcattaaag acagtgtttc ttataccaac ttgtttgtcg gaaaagaata caccatttca
     4741 ggagtattga tgaacaaggc aacgaatgcg ccaattttac aagatggcaa agaaatcaca
     4801 agtaccttgg cctttattgc agacaaagtc aatggcacaa aagaccttga atttgtggtt
     4861 aatgctagtg ttctgaaagg tcaaacgatc gttgtttttg aaaaactttt ccgtgatggc
     4921 attgaagttg cagcacatgc ggatatcaac gatgaagccc aaagtgtgaa agtgaataat
     4981 ccaaccgttc ataccacggc tgtctttgaa gacggattga cagtcgcaga cccaacagga
     5041 ttgtttacct taaacgatac cgttgaaatt accgagattc ttccaggaaa agcatacttg
     5101 gtcaaaggga tcttaatgga taaagcaacc ggtgaaccct tgcttgtgca tggaaatacg
     5161 gtagaaacga gtgttggctt tattgccaaa gcagaaaatg aaacggtgat cgtaccattt
     5221 acctttgatt tgacaggtat aaatggtcgt gaaatcgtcg tgtttgaaag tctttttaga
     5281 aatgatgaag aactagcaaa ccataaagac atcaacgaca aagggcaaac ggtacgagta
     5341 actaacccaa gtgtacgtac aacggctaca cataaaccca ctggcttgaa agaggttaat
     5401 ccattaacga aagtcaccat tacagacgct gtgaaatacc acgatttggt cgttggaaaa
     5461 acatacacag tcaacggtgt tctaatggac aaagtaacgg aaaaaccagt gctagtagat
     5521 ggaaaagaag tcacaagtac cttaacgttt gtggcagaaa caacggatgg tacagtcaat
     5581 ctagacttta ttctagacgc acgtgcgttg cgaggcaaag aagtcgtggt ctttgaagat
     5641 ctgtttagag aaggcgtatt attggcttct cacgcagata ttaccgacaa tgaccaaacc
     5701 atcaaaatca gtaatccaac cattacgacg aaagcgacga ataaaaatgg cggaaaagag
     5761 atcttggcct tgccaaatca aactgtgctt gaatgggtca aagtagacgg tcttgcgatt
     5821 ggtcaaatct atcgcttaat tgttcaaggc tacttaacac cagacggcac accatttgaa
     5881 ggcactcatg cggaaaaagt ctttgcggcc gataaagaaa tgatggaatt cttatttgaa
     5941 ttcttagtgg acggtcgcaa tcttgcagga aaaggcttga gttttgcgga atggctccaa
     6001 gcgaaaacgg aagatgagaa agaaacgttt gaagaagtgg cgaaacacaa tgtagatcta
     6061 acaaacaaag atcaaacggt tcaaattgtg gaagcgccaa aaccaccgca accagtgaaa
     6121 ccagaacaac gggttaaacc agctactgtt gtaccaatgt tgccaaaaac aggtgaacaa
     6181 acaagtagta tacttatggt tcttggatta gtagtattag tcattgcttt aggtataaca
     6241 ctttataaag tacgtaaagc caaagattag tcatgaggaa caagcggtta ggatttttaa
     6301 ccgcttgttt gattgtaatg tagaaatatt tagaatttat tttggttttt aagaggagga
     6361 aaaactttat ggcagaaaaa gcattgcgta tgaagaaaga gtttttaggt caagtatttt
     6421 tattgggttc acgggaacct aatccaccaa aggaaattaa tggtgtgtct gggcgatatg
     6481 ttcaattaca agcagagtgt tcgcgagaag aacagcctgc tttttttgtt tatctcacag
     6541 ctgaagagga taaaagatta cgattaccag aaaaagaggt gcgtatttca tttgaagaag
     6601 cttattttgt ttttcgtaaa gaacgagtga atagtcgtga aacaaatgat atgggacaaa
     6661 cagagcgtcg aacaactggc tatcaagatg ttggtgtctt tttaactggt gtaaaaattg
     6721 gggaggtttt gtaagatggg attaaaattt ccaaataatt atgacattac agaagcatta
     6781 gatgaaactt gtttaggagc cgtacgcttt ggcgcattag aagagtctga tcgtgtattt
     6841 gataatgaaa caggcgatta tacgggagaa attcgatcac agcgagcaca attagttttt
     6901 ggaaatccag atgaaccaaa gattgcacga cgagttaaat ttccagcaga tgtagatttg
     6961 actggtttta aacgacgtga tcctgtgaaa ttaattgatc cagaatatca tgtggtatat
     7021 attccagcac aagatggcaa tcgtgaatat tcagatttag tgatttatgc gaaaggatta
     7081 gagaaagtag aagctccaac aaataatcct aatatgaaac aatcacaaga aaaaggttta
     7141 gatcaaaaac aaccaagtgt tgattctaag gaaaaagaga aaaaataagg tttttttgaa
     7201 agaataggaa ggagtcttcc tattcttttt atctaagata gaaggaaggg gagattttga
     7261 attcagataa aaccaatcaa atactttgag agattgtgct tgttatcaaa atatccaaca
     7321 attgattgag tattttggag acaatccaac gatttttgta caaacaaatg catatatttg
     7381 tgcggttatt gggcagtgta tgtttttacg tgatcttgtt cgtttttcat taccgtacca
     7441 gtggaaacaa gatcatcagg aagtgatttg gattcgtata gtacgaatat gtaatatgtg
     7501 tgcacaccct tataaaaatc gattaaatct ttttcgatta tgttttttta ctaaatgtga
     7561 tattcctgta ttagttgctt tttgtcaaaa gtttttagca caggaaaag
//
LOCUS       DACDCJ010000108         7449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDCJ010000108 DACDCJ010000000
VERSION     DACDCJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.109"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            77..1225
                     /gene="tnpB"
                     /locus_tag="IV038_002582"
     CDS             77..1225
                     /gene="tnpB"
                     /locus_tag="IV038_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP8342743.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            1747..2028
                     /locus_tag="IV038_002583"
     CDS             1747..2028
                     /locus_tag="IV038_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342744.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            2030..2446
                     /locus_tag="IV038_002584"
     CDS             2030..2446
                     /locus_tag="IV038_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342745.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            3068..4222
                     /locus_tag="IV038_002585"
     CDS             3068..4222
                     /locus_tag="IV038_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP8342746.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            4216..4638
                     /locus_tag="IV038_002586"
     CDS             4216..4638
                     /locus_tag="IV038_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342747.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            5808..6776
                     /locus_tag="IV038_002587"
     CDS             5808..6776
                     /locus_tag="IV038_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8342748.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            6793..7317
                     /locus_tag="IV038_002588"
     CDS             6793..7317
                     /locus_tag="IV038_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8342749.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT         2803 a         1257 c         1244 g         2145 t
ORIGIN      
        1 ggcgcaccaa ttgatgtgat taagaagtat atccaaaatc aaggaaacaa tcataaatag
       61 aaagtaggtg aactttatgg aacaactaaa agcatacaaa ttcagaatct atccaacaga
      121 ggaacaagaa atcttctttg ccaaatcttt tggttgtgtc cgtaaggtct acaatctaat
      181 gcttgatgac cgaaaaaagg cgtatgaaga agttaaaaat gattcttcta aaaaaatgac
      241 ttttccgaca ccagctaaat acaagaaaga atttccgttt ttgaaagaaa ttgatagcct
      301 tgctttagcc aatgcccaac ttaatttaga taaagcatac aagaattttt ttcgggataa
      361 atccgttgga tttccacgtt tcaaaagtaa gaaaaatcct gttcagagtt atacaacgaa
      421 taatcaaaat ggtacagttg ctttgattga tagtaaattc atcaaagttc ctaaattgaa
      481 gtcattagta agaattaagc ttcatagaca accaaaaggg attatcaaat ctgctacaat
      541 atcacgtcat tctagcggta aatactatat ttctttgttg tgtaaagaag aaattatcga
      601 attacctaaa actaattcca caattggtat tgacttaggt attatggact ttgcgattct
      661 ttctgatgga caaaaaattg ataataataa attcacgtcc aaaatggaaa agaaactaaa
      721 gcgtgaacaa cgcaaattgt ctaggcgtgc tttattggct aaaaataaag gtaccaatct
      781 atttgaagct aaaaattatc aaaaacaaaa acgcaaagta gctagattgc atgaaaaagt
      841 aatgaatcaa cgtactgatt ttctgaataa gttgagtaca gaaatgatca aaaatcacga
      901 tattatctgt attgaagact taaacacaaa aggtatgttg cgaaatcata aattagctaa
      961 aagtatttct gatgtttctt ggtcaaagtt tgtgactaaa ttacaataca aggctgactg
     1021 gtatggacga aaaatcatca aagtagataa atggtttccg tctagtcaga tttgttcaga
     1081 gtgcggacat aaagatggca agaaatctct tgaaattcga gaatggactt gtcctgtttg
     1141 tcatactcat catgatcgtg atatcaatgc tagtatcaat attctgaccg aaggtctaag
     1201 actacattct atgggattgg cttagatcca atcatcaaga accgtaggaa ctacggggat
     1261 agcttggtaa ataagagaca ccgctgttag taaagaaata cgctatcaag tatgctctat
     1321 tcccaagaag ctcccacttc aagcgttaag aaccgtcagg tttagctaag tggtgagtag
     1381 ttcacattaa taaaaattgc tggttttatt cttctttttg cttttgcgag cagagcaaca
     1441 tggacccatg ttgcgatatt ttggtggcgc cccaatcccc gttttttcgt tgaatcttac
     1501 tcggactgca ctcgttttac agctgatttt tctgaatttt gaaagagcat aaggaaacgt
     1561 ggtatcactt tcttacgaaa ggtgctagat aggctgtgcc tatcacgttt ctcagaaaaa
     1621 aatttgctat cacttttttg ccattgtggc taggctatgt atagtgtttt ggcgaaaaat
     1681 agctatacaa atactatatt tgtatggtat acataaatta tacgctgata cgaaaggaaa
     1741 atttccatgg acattttagt tcgaaatatc aatcccaaaa ttattgcaag aatagataaa
     1801 cgttgtcagg aactcacaac caaaacgggc aaaaaatgga gtcgaaataa atatctaaaa
     1861 gttctaatcg aaagtgattt tgaccgtcaa cttatgaatt acaaaaaaga tcaattcgat
     1921 ttattactcg aaaaatttat tgccattcaa acagaaaata cgaacatact gaatgactat
     1981 gttcacacaa acaaccaact cattgaaaca cttatcggag gaatataaca tgagaaaaac
     2041 ttctgttcaa aaacacgtaa ccctttatcc cagtcacgtt gacgttttag attccatcat
     2101 cgaaaaaaat ccagacgtca acaattattc agaagccatt cgttttttat gtatgagtta
     2161 tgaacctgat accaatacaa ccacaaccag taaccaagca aaactcaatg ccatgggaaa
     2221 agaaatttct atcatgacag aaatcatggc agaatttgct gatttatatc taaaagatac
     2281 tggtgttctt atcggtacaa attctaaagt gtatcaagaa gtgaaagaaa aaattgaaaa
     2341 tcaactcaat ccttcccgac gaaatcaacc caaaaatcgt caaaaagaaa ccaatcaaac
     2401 ctttacttct ggaaattttg acccaacaaa ctttctaaaa agataaaaaa agaaaccgat
     2461 ccctaaaaaa attcctcgat ttctttttag ggatcagtct tccagcaaga tttgcaagtt
     2521 ggtaccaact tgcgaaaaaa aagaaaaatc cgcttatgca acttcttctt tttgtatttt
     2581 gaggcgctac cccaatcccc aagtatcggt tatttaatag cgcggaaatc caaagaatag
     2641 gacgtaccct gaaacaaaaa aagactgctc tcacgagcag cccaaaatga gattatccta
     2701 ttcatcaaca taaaaatatt acaaagtgaa gaactagaga aagacaaaaa aagcatatca
     2761 aatcctctca aaaataacaa gtctttaaaa aatgacagat aaaaatgcac aaagaactaa
     2821 aaaagagaaa tcgcgtaaaa tcaagcatta gaagctagta actagctaga ccaattaaaa
     2881 atcacaaagt gagcgtctct atccattggt agaggggcta cttggcatag tgtacattat
     2941 gttaactaac gctataaata acgattatgt tcccaaaaca atattttatg aactataaca
     3001 aaaatttgtt atcgcatatt gtatatttta attataggtg tataccactt aaatttaaca
     3061 caaaggcgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc tcttttcctt
     3121 gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa attctaattt
     3181 caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa ctgattttga
     3241 acactccata acttcaattc ttttaatgga aaataacacc gaaactgata ttaactatac
     3301 tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt ctattttctt
     3361 tccagtattt tcagacgctg ataatacgat taaatatatt tataccgtca aaaaaaatac
     3421 aaatggagaa tattcaggaa gtatttcaca atttttagca aaagaacttc aaaatctagt
     3481 agatagtaat ataacagata caaccttatt ttttcaagat aatagcattt attattccga
     3541 taatggacaa attaaactac tttggaaatc acctttaagt acagaaaaaa atggtgtttc
     3601 ttcctttaca gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa ttaaaaattt
     3661 ctctaaaaaa caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct ccggagataa
     3721 ctttattatg attaattggc gtatagcaga aacccaaaca agttttccgt ggtgtgctgc
     3781 ttacgtagaa gctgctatct tatgtaacaa aacagatgta cgtgctactt cagctagagc
     3841 cattatgcat tatacctatc ctaatttaaa caacagtcaa attgaatcaa aaagtcttaa
     3901 tcaagatcaa ataataaaat atgcaaatta ctgtggtgtt tatcctaaaa aagtagcacg
     3961 aatactaaac ctttcagagg tccaaacaca actaagaaaa ggaaatgcca tttatatggg
     4021 aactgctggt tatggaaatt atagtaacag ccgtcatgca cttgcattat ttgggtgggt
     4081 caatcaaggt ggttatcaaa cttactatgt gtggaatcct tggtataact acccaactgt
     4141 aataagtgca aatgcaggac cagttacaat tcctgtacct ggaggtggtt ttacttggga
     4201 caataccatt acaaattggt aaataaaccc attgctttag ttatgggtct agtgttagtt
     4261 cttttcttaa ctagttgttc aaaaaaacaa gaaatacaaa agactgatct gattcctttt
     4321 ctaggaactt ggcaaactaa agatcattca gaacgtctag taatcactaa tttaaatact
     4381 tcaaaaaata tagaacaata taccatctta gtaaaagaga acaatcataa aattcaaatc
     4441 attgataaaa atttaaatat aaatgaacaa gttttcattt acaaagcaac aaataattta
     4501 ctaacactgc gctctaataa aaaacaatta gattacactt ttagtttgga ttctccaaaa
     4561 aagctcgtat ttattttgac aacgaatgat gggacagaag ggacagctaa accaattgaa
     4621 ttttttaaag ctgattgatg aatagttgtt tgatattaca aaacaataat ttcaccgaag
     4681 gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagcctttt ttgtattgtg
     4741 gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaaa agcggttatt
     4801 tgcaccaaat acgtaggtat ttggctagca aatatagagc ttgccaaaca ctttagtgag
     4861 ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc cgtaggacgt
     4921 actgaaaggg tgggatttat gagcgaacta atgcaaaatt tggctagcaa aaaaagttaa
     4981 aaaatatatg ctaataatcg agcgaaatga acactttttc aagtgagcga atccgccatg
     5041 acaaagctga agcagtttat ttcagctttg tcatggcgtg tgcctagact tttaaaaaag
     5101 tcggcacacg agcaaatatc tttatcaagc tctaatttgc gttttaagac gtttgaaacg
     5161 tctaagtata taaatatata agagtaggtt taagagtgct taaatcgaat tttagagggc
     5221 attcagacaa ctttagggtt gttagggctt gccctgaccg cctgtaaggc gctttaagaa
     5281 caagataagg gatttatact aagactgtct taaaacgtct caaacgaagg ctcatagctt
     5341 ctcaaagagt tccttatgaa catttttagc aaaggagccg gaagcgactg cgcagtataa
     5401 tgtgagagca gagcgatcac acctttttaa gcgactgtag ggagtaagat tcccagttga
     5461 gaaaaatgcg cgaatctgtt tggagaaaaa agaagtggtt ttttaggcgg gaaattttgt
     5521 ccccctcttt tataatctct ttttaaacct ctttttaaac ctctttaccg tcggctatat
     5581 ccttactctc ccaagggaat gacgctttta tctacatcgt tttgtctgtt tatctacatc
     5641 gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttgta
     5701 cattgaatta gtacatcgac gatgttaata taagatgtag ataaacaggc attttgtatt
     5761 tcataacgtg tatctcttgt gcgagtaaga aaggagctat tttaaatatg tctaatgaaa
     5821 tcgtaaagta tcatcatgaa ttaaatacaa ttccgttgcg aaaatttaca ccagtcgaaa
     5881 tgaatttgtt cttttctatc gtatcaagaa tgcgtgatgt aggtgataaa aaagtacaat
     5941 ttacattcga gcaattaaaa gatttaagca attataaagc aactgcaaat gttcgattca
     6001 tagatgattt agaaacaacg tatgacaaat taatggattt acgttttggt agaagaagtg
     6061 ctgacggttt gcaacgtgaa cgttttgttt tatttaatca atttaaaatc gacggaaaat
     6121 ctgatattcc ttttgctgaa atacaagttc atgaaaaagc cttaccctta cttaataatc
     6181 ttgaagaatg ggtgcgctat tctctccaac aattcaatga attagaaagc agttattcaa
     6241 aaacaatgtt tcgattacta aaacaataca gaaccaaagg tttcgcttat ttttcaaaag
     6301 aagactttca tgaattactt gatattccta aaagttacaa ccaaggtacg attgatcaac
     6361 gtgtgttcaa tccaatacga caagaactaa ccgcaatctt taaaggttta acaatcaaaa
     6421 agaaatacgg aaaaggacgg ggaaagcctg tcattggcta tcaatttaca tttaaacctg
     6481 agataaaaaa atcagacgac tttaacaaac atgccccaac aaaagaagaa caaaacaaag
     6541 cattagaact agctaaccaa caagttcaat ctgaaaaagc acctacttca ttacaagaaa
     6601 aaatcaaaga acaaaagcga aaattggaac ttttacaaca gttagaaaag ttagaaagag
     6661 aacaaaatac ccaaaataaa gaaaataatg cacaaaaatc agagataaat tccgatattc
     6721 cttcagaact aaaaaaagac ttattagaca atcttcaaaa cttattcaaa gattgagggg
     6781 tttttagatt ttatgacaga aaaaacaatc aaagaacttg ctgaagaatt aggagttagt
     6841 aaaacagcaa ttaacaaaaa agtttctgac gaaaatcgaa aactttggtt ttcgaaaatc
     6901 ggaaacaagt ttgtgattaa tgaaaccgga caaaaggcta taaaatcaat gttttttaac
     6961 gaaaacgaaa actcaaatca acaactagtt ggcgaaaaaa cgaaaaccga aaaccacaaa
     7021 aacgccaacc aagttcccga attagaagtc ctttctctcc tagaacaaca gttaaatcaa
     7081 aaagatatgc aactacaaga aaaagacgag caaataaaac aaatgcaaaa attactcgac
     7141 caccaacaac aactcaccct gcaagccaac caacaaatcg agaaactgca ggaacaacta
     7201 cagttgactt atacggaaga atcttcggaa aataaatcaa cgactctttc tgaagaaaca
     7261 gaatcgatag aaaaacaatc aaaggcggag aaaaaatggt ggcaattctg gcaataaaac
     7321 cagcaattta acagaattag gcagaagtcg cccatcctct ataggtgggt gatgaatgcc
     7381 gttcggtatg agggttaccg ttggtaactc gaagaccgac aactaaggcg tattgccttt
     7441 ttattttgt
//
LOCUS       DACDCJ010000109         7277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDCJ010000109 DACDCJ010000000
VERSION     DACDCJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.110"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(32..1438)
                     /gene="lpdA"
                     /locus_tag="IV038_002589"
     CDS             complement(32..1438)
                     /gene="lpdA"
                     /locus_tag="IV038_002589"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP8342750.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            complement(1446..3089)
                     /locus_tag="IV038_002590"
     CDS             complement(1446..3089)
                     /locus_tag="IV038_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP8342751.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            complement(3114..4091)
                     /locus_tag="IV038_002591"
     CDS             complement(3114..4091)
                     /locus_tag="IV038_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP8342752.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(4094..5203)
                     /gene="pdhA"
                     /locus_tag="IV038_002592"
     CDS             complement(4094..5203)
                     /gene="pdhA"
                     /locus_tag="IV038_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP8342753.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            complement(5664..7088)
                     /locus_tag="IV038_002593"
     CDS             complement(5664..7088)
                     /locus_tag="IV038_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP8342754.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         1889 a         1630 c         1307 g         2451 t
ORIGIN      
        1 tttcagttgc gtttcgcctg tattattcaa atcaaatatg gattggtaaa cctaatgcta
       61 attccgaagc atccatgacg atttcgccaa gagatggatg aggatgaatc gtcaaggcga
      121 tatcttcagc attcattcct gattcgattg ctaaagctaa ttcagagacc atatcacttg
      181 cgccgactcc accgatttgc gcgccaataa gcacgttatc ttcgatcgtc gtaactagac
      241 ggatgaatcc ttctgtttta ccaagcgata atgcccgacc gttccctgaa aatgggaatt
      301 tatatgcttt tgcttcgata ccagcatctt ttgcttcttt gatcgtcata cctacagaag
      361 ccaattcagg atcagtgaag gctactgccg gcattgcttt ataatctaca gctacttttt
      421 tgccagagat tgcttcagca gcgatttttg cttcataact ggctttgtgt gctaatgccg
      481 cccctggaac gatatcaccg atggcataga tgttaggtac gttcgtacgt ccttgtttat
      541 ccaccgtgat caatcctcgt tctcctactt cgacacccgc ttgttctaag cccatatcat
      601 ctgtattcgg acgacgacct acagtgacca tcacataatc agcagtaacg gattcttctt
      661 tcccgtccac tgcatatttc actgtcacgc tgtcaccgtt atcgactgct tctttcgcca
      721 ttgcattcgt tacgactgtt acgccttttt tcttgaaatc atcttctact aattttacta
      781 gatctttttc gtaagttggt aaaatttgtg gtgagccttc aagaatcgtt acttctgctc
      841 ctaagtttgc gtacgctccg cccaattcgg caccaatcac accgccaccg atgatcacaa
      901 atttcttagg tacttctttc aatgctaatc cgccagtaga atctaacaca cgtcctccga
      961 atttaaatcc tggaatctcg attggacggg aacctgttgc gatgattgcg ttattgaacg
     1021 aataagtttg agctgaatct ggatgaatga cgcgtagtgt gtgatcatct acgaagaacg
     1081 cttctccttc gattgtttca accttatgtt tttttaatag gaaaccaaca ccagaagtca
     1141 acgtatggac aactttgttt tctttccatt cttgtgtttt tgcaaaatct agtttgacgt
     1201 tttcactagt tactccaaac atttcagaat ctagtgactc ttgataatgg tgtcctgcag
     1261 ctatcagtgc ttttgaagga atacagccga cattcaaaca aacaccgcca atatactctc
     1321 tttcaatgat tgctactttt tgtcccattt cagcagcgcg gatggcagct acatagccac
     1381 caggtcccgc accaattaca actgtatcta attctacagc aaaatctcca actaccattt
     1441 gtttatcatc cttccatcat taatagctct ggatcagcta aaagacgttt gatattattc
     1501 attgcttgtt gcgcagttgc tccatctacg atacggtgat cgaaacttaa tgacagtttc
     1561 atcacacgac cgacaacgat ttcgccttca gcatttacga ttggttgttg tgcaatcgtt
     1621 cctacaccta aaatagctac ttctggatag ttgatgactg gtgtaaacca accgccacct
     1681 acagatccga tattgctgat cgtaattgtt ccattgcgca tatcttctgc agatagtttg
     1741 ccgtcatgag ctaattttgc tttctcattg atttcgtctg caatagcaaa cattcctttg
     1801 cggtctgcat ctttcacatt tggtacatat agtccgtgat ccgtatcagt tgcaataccg
     1861 atattgtagt aatgtttata aacgatttct tggtttgcat cgtcgataga tgcattcaat
     1921 actgggtatt tcttaacagt cgctgttaaa gctttcacga catatggcaa gaaagtaagt
     1981 ttcgtaccat ttgcagcagc aacttcttta aatcgtttcc gattatccca tagtttagac
     2041 acttctactt catcatgcaa tgtcacgtgt ggagctgtat gcttactgtt gaccattgct
     2101 ttagcgatag ctttacgtgt tggcgtcatt gctacacgtt cttccaagtc tcctaagttg
     2161 gacttgaatg gttttgctgg tgcagctggt ttgctttctg ctgcaggagc tgcttctttc
     2221 ggtgctgccg cttctggcgc ttctgatttc gctggtgcac tgcttggacc acctgccaag
     2281 aagttttcga tgtcctcttt tgttactctg ccaccttttc cagttgcagt tacttgtgag
     2341 atatctacat ctttttcacg ggcaaattga cgaacagatg gcatagccaa tacacgttta
     2401 tttggatcgg cagcttcaac gacacttgct gaacctgatg tttccacttt ttcttttggt
     2461 gcttcagctg atggcgcact agttgacggt gcactattgt gtccaggagc atcgatttct
     2521 actaatacat ccccaacatt tgctacagtg ccttctggaa caatcacgtt tttaactgtc
     2581 cctgtgacag gtgacggaat ttcttctact gatttgtcat tttgaacttc tagtaatgta
     2641 tcatcttcat tgatcgtatc tcctggtttg acgaaccatt tgacgatttc accttctgcg
     2701 attccttcac cgatatccgg taacttaaat tggaagactc cttcgcttga cgatccagct
     2761 gattctgtat caacagtggg ttcagcagcc ggtttagctg gagtttggga ttctgcggct
     2821 acaccactgt ccccttcatt gtcttcgtgt cctggtgcat cgatttctac taatacatcc
     2881 ccaacatttg ctacggtacc ttctggaaca atcacatttt taaccgttcc tgtaacaggt
     2941 gacgggattt cttctactga tttgtcattt tgaacttcta gtaatgtatc atcttcattg
     3001 atcgtatctc ctggtttgac gaaccatttg acgatttcac cttctgcgat tccttcaccg
     3061 atatccggca atttaaattg ataagccatg actttgtttc gtcccttctt ttcttaaaat
     3121 tccgcgattt cttttacttt gtcttcgata tcttttgcat ttggcaacca gacattttct
     3181 gcttgtccaa atgggaagac tgtatcaggt gcagaaactc gtccgattgg ggcttctaat
     3241 gaaaggatag cgcgttctga gatctcagaa actacttgcg cggcaacgcc cgcttgtcgt
     3301 tgtgcttctt ggacaacaac tacgcgacca gttttttcca cggattgaat gatcgtttct
     3361 acatctaatg gcgcaactgt acgaagatca atgatttcaa cagatatatt ttcttttgca
     3421 agattgtctg ctgctttgat tgcttcacgc accattgctc cgtatgtgat aatagataca
     3481 tctgttccct cacgagtaac tgctgcttta tccaaaggaa cttcgtaagc ttcatccggc
     3541 acttcttctc ggaatgagcg atatagtttc atgtgttcta ggaaaacaac cggatcgtta
     3601 cttctaatag aagaaatcaa tagtcctttt gcatcataag ggttggaagg aatcactact
     3661 cgaataccag gagactgagc gatcaaacct tctaaattat ccgaatgcaa ttctggtgta
     3721 tgaacaccgc caccaaatgg cgaacgaatt gtgatgggta gatttctagt gccgctcatt
     3781 cgatatcttg tacgagccat ttgagctact acttcgtcca ttgcttcgaa aataaaacca
     3841 aagaattgga tttcaggaac tggacgaaat ccttctaacg caagaccgaa actcaatcct
     3901 gcgattccag attcagctaa aggagtatcg aatactcggt cttctccgaa cttttcctgc
     3961 aatccttcag ttgcccggaa aactcctccg tttttcccaa catcttcacc aaaaacaacg
     4021 acattctcgt cattttctag ttcaagtgct aaggcatctg tgattgcttg gatcattgtt
     4081 ttttgtgcca tgattatttc gactccttcg cttcataaat tgcaatttgt tctttgattg
     4141 attgtggaga tacttcgaac atgtttttca agaaatctga aaccttttgt tttggtactt
     4201 tgtcggcttc agcaatagct gctttgattt cttctttagt tgcttcgatg acttgttctt
     4261 ctttttcttc agaccaaagt cctttttccg tcaaatattt acggaagcgg atcaatggat
     4321 cttttttctg ccaatcgtca tctgtgtctt ttgtacggta gcgggttgga tcgtcaccag
     4381 atagtgtatg tggtccataa cgataagtca gtgtttcgat taggactggt ccatttccac
     4441 tgatcgccca ttcccgtgcc attttagata cagtatatac tgcaagcgga tccattccat
     4501 cgacttgaat accaggaatc ccagctgcga cagctttttg tgcaagtgtt tttgctgcag
     4561 tttgtttttc acgaggtgta gagatcgcat acccgttgtt ttggatataa aatacggcat
     4621 ttgcatggta agcgccagcg aaattgattg cttcgtaaaa atcaccttgt gatgaaccgc
     4681 catcaccagt ataagtgaag acgacatttt tcttgtttcg tttcttcaaa cctaatgcaa
     4741 caccggctgc ttgtacatat tgtgcaccga tgataatttg tggtggtaga gctttcaatt
     4801 cttccggata agaattacct gcagcatgtc ctcgagacca taaaaaagct tgagataaag
     4861 gtaaaccatg ttttactagt tgaggtacat cccgataacc tggaagaaga acatcttctt
     4921 tatcaaatgc gtaagcacta gccagctgac ttgcctcttg tccagcagtt ggtgcaaaaa
     4981 agcctaggcg tccttggcgg ttcagcgctg ttgaacgttg gtctaataca cgagaccata
     5041 ccatatgagt cattaattca acgagttcat catcggataa atcaggtact aaatctttgt
     5101 taactacgtt tccatcttga tcaagtgctt gatatactgg gaaatctgca ttgacttcat
     5161 ctagcagtgc ttggaagtcg atcggtgctt ttttgtttgc catttgtcac acattcctct
     5221 ctcatctata agaaaatctc ttcattctgc gattgaaaca gtgaataaaa ctgtatcact
     5281 atttaaccgt cttcattaat aaattatcat ggatttatga gaaatacaag ctaaaagcac
     5341 aaattttcac gatgaaagga aattatgcca atccgcatat taacagcatt agtgaataaa
     5401 atcacaataa ggaaagcgtt ttatatttca tctgcatttt ttctattttc taatcaaact
     5461 tacaaccatc tgtttctatt taaaaacgtt ggtgttatac agttttgttg caaacattta
     5521 ttttatttac acatcgtgaa cggacgtaca aataatggtt ttttcttagt ttctttatat
     5581 cgtattcttt ttccgaaaaa aaagaaacag cttgaaattt aaatccaagc tgttatattc
     5641 tttaatctat ttactttgtg aaattattct gttaaccact tttctagaat tttcatcttt
     5701 gaataaaaca acggtatttg gttttcatcc agcctactgt aattgattct gattccattt
     5761 atatcttgcc caaaaagtag acttggtgca attccaagac ccatatctac tactttttct
     5821 gctagttcgg ggtctagttt tttcccatcc caagtaatcc aggcatagta accacctaaa
     5881 attgaatgaa gcttgaaaaa cgattgtgaa tctccccaag cgcttatcag tcggcttttt
     5941 tctttcaact tttttcttac atcttgcatt ttcaaatcaa ttgcttcatt aaatacatta
     6001 gccactgtga tttgtgtaag gatattcatc gaaaactcca tcatttcttg cgcttgagct
     6061 agttgcgaga taaaagtctc aggtcctatc acccaaccga ttttcgttgt ttttcctaaa
     6121 accctagata acgaaccgac gtataacaca ttatccggat caatcgttcg tataggaagg
     6181 acttcttctt ggtttagaaa tgaaagatcc gtaaatacat cgtcttcaat aattggaagc
     6241 cgatactgtc gacacaattc tactagcttt tctcgtcgtt tttgactcat cacttttcct
     6301 gtagggtttt gaaaattcgg gttgataagc actgctttca atttattttt atggatcgat
     6361 tctttcagtt tttctaagtc gatcccttct tcatccatcg gtatcccaaa taaacgcgtt
     6421 cctgaagctt tgaaaagagg taatcggtaa aaaaaagaag gtgactctac agctatcccc
     6481 tctccagctg aaagaatcgt ttgtaaaatc aaaaagattg cttgctgacc gccaccagtc
     6541 acaagaagct ggttggggga aaatgccaat tcaaatttac tttccaaata ccgtttgatt
     6601 ttgttacgta acggagtata gcctaaaaga tccatttctt tttcttccgc taagatttct
     6661 tcaagagaat aagaaggaaa ctcaaaattc ggcaaaagct catctggcat ctcgctagcg
     6721 tataaatcaa gaaacgatgt tttttggatc tgctttgctt ctcttatttt ttgctcatac
     6781 cagtccgtct gctcataacg aggagagatc atttgccgcc aatcgattct tggaacagcg
     6841 aatttccccc attcagtctg attaacaaac catccgctgc cacgtttgct cgttagtacc
     6901 ccaagctccc taagctcttc caatgcatgg acaacggtgg aacgattcac accaaaaagt
     6961 tgccctagtt ttcgttctga ttcaagttga cttccaggat tgatccttcc gttttcgatt
     7021 tctctcataa tctgttccat aatttgacga taaatcggtc cttgtttttt ctctactttt
     7081 tcccacataa gcttctcttt tctattgtct attttttctt ttgattttac cgaatttacg
     7141 agaaaaaaga aaaacttttt ttgcaaaaca taagggactg tgacaaaaat cttatcacag
     7201 tcccttattc tgaataatga tgttcaaaag cttgtatctg cgataataat tccagccaaa
     7261 caaaacatat aaagaac
//
LOCUS       DACDCJ010000110         7197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDCJ010000110 DACDCJ010000000
VERSION     DACDCJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.111"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(16..768)
                     /locus_tag="IV038_002594"
     CDS             complement(16..768)
                     /locus_tag="IV038_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8342755.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(1132..3798)
                     /locus_tag="IV038_002595"
     CDS             complement(1132..3798)
                     /locus_tag="IV038_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP8342756.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4140..5591
                     /gene="cls"
                     /locus_tag="IV038_002596"
     CDS             4140..5591
                     /gene="cls"
                     /locus_tag="IV038_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP8342757.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(5796..6611)
                     /gene="thiD"
                     /locus_tag="IV038_002597"
     CDS             complement(5796..6611)
                     /gene="thiD"
                     /locus_tag="IV038_002597"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP8342758.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            6905..7180
                     /locus_tag="IV038_002598"
     CDS             6905..7180
                     /locus_tag="IV038_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342759.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         2106 a         1507 c         1231 g         2353 t
ORIGIN      
        1 cataaaaaga acccttcacg agtcgtgaaa ggttcttttt ttatctgcgg accaaaggag
       61 cagtctatag gtcagcttag tcgtactaag gatttctcgt ttttcactga atagtttgcg
      121 aatgtacctg gtcacaacag gatcttttgc gtacgcgtca tagtatagct tcatcaattc
      181 ttcagtaatt gattcctgtg taaaataagt aaaatctttt ttcttcactt tgaatccttc
      241 atcaattaag tatgtttcaa attgatccat tacaactggc gatgtattag gatcttcttc
      301 tatcattcct agttgctttt cgataggata aaaaacatca tcgattgatt ccactaccct
      361 gattatcacg caccatttgc ttgacttttt catcaatttt tctaattgaa taagatgtac
      421 caatgctgga tttgctgctg taaaaaccat ttcatatgtc tcatttgatt ggataaattc
      481 ttctaacgac tgtttgataa aggaaacatt tggcaactca tttttttctg cttctttttt
      541 tgcattttct agcattcctg aagaaaaatc gatcaaatca tagcgattaa tttcttttgc
      601 taaggaaaga aatctgccag atcctccagc aacgtctgct gctgtttcag caggcaggac
      661 tttctccgtt ttcagataat tttttacatc ttcagcaata gttacttgcg attcttgctg
      721 gatttcataa tattcttcag caaattcatc ccattctttt tcattcattt gatttcctct
      781 aattttttgt tatatgtttt cacttttcgg aataaattat tctcctcttt tatctattct
      841 atctcttttg atgaataata aaatgattgg ctttccgtct atcataaata aaaaaaggga
      901 ctgtgaccaa agtcgtgtca aagtccctct ttccgaataa acagtgtcca atcagatgac
      961 cctcagtatt agctcaaaat gctgatttta caacttcttc cttaaacggt gttctcctgc
     1021 caactcttcg gtcacaagtc acaatattca acaaacatga gaagctgttt gttgaaattg
     1081 caccttgtga ctcagaatta aacggcaggc tcacaacctc gttgattcgt tttatttatt
     1141 tccttgtact cgtacaatga tcaatttagt gatttccatc aaaacaattg tcacaagtgc
     1201 gattcccata catgtaaaga cttctgtcaa tccaaatgct gatgggatag agaagacttc
     1261 tttcagtcct gggatcattg ttaagcagta aagcgcgcta catacaacta cagctagtaa
     1321 gacacttctg ttagagaaga accctgcttg gatagctgtt tgtgtgtttg aacgtgctgg
     1381 gaatgtttgc aatgtccgac accaaatcaa tgttgaaaat gacatggcta caccgaattc
     1441 aggtgaatgc tgcatgccaa gccattgagc aaagataaca gcaatcccaa tcagcacacc
     1501 acgatacgtt acagaaatca atgttttgtc cgtaaagatt cctgaatttg gatctcttgg
     1561 cgggcggttc atcgtagcag gttcagcttt ttctgtacct aatgcaattg ctggcagtga
     1621 atcattgact aaattgatga acaacagttg taatgctgta aatggattgg accagtcagc
     1681 aactaaggca aagatgatcg caatgatcgc acctaaattt cctgcaaata gataagaaat
     1741 agctttcttg atattatcaa acacattacg tcctacttct actgcgctga caatagaagc
     1801 aaagttatca tccgtcaata ccatcgctgc tgcatctttt gccacatctg tacccgttcc
     1861 catagcaatt ccgatgtctg cttgtttcaa tgctggtgcg tcatttacac catcacctgt
     1921 catagctgag atatgacctt tattttgcca agcacgaacg atacgaattt tattctcagg
     1981 tgaaacacgt gcataaacag tgactttttc taaaacttcg tttagttgat cttctgtcaa
     2041 ttcatctagt tccgtcccgg tcaaagccag gtttccttct tcaaaaatcc cgatttgttt
     2101 tgcgatagca acagctgttg ttttgtggtc accagtaatc atgattgttt tgattcctgc
     2161 acttcttgct tctgttaccg cctgagtcac ttcagcacgt ggaggatcga tcatagcaag
     2221 caaaccaaca aggatcaaat cgtcttcatc ttcaaatgat aaatcaaaat tatcaagtgg
     2281 acggtaagca aaagctagta cacgtaatgc gcgtgttgaa aaagcttcat tttgctcttg
     2341 gaattttttc atcaattcat ccgtcatcgg aacgacttca ccatttagca atactttttt
     2401 actgcgttgg aagacgatat ctggtccgcc tttgacaaac aagtaatttt cgttatcgat
     2461 cgtgtgtcca gtagacatta attttcgatc agaatcgaaa ggaagttctg cttgtcttgg
     2521 gtagttgttt cggatttcct gataaggctg gtttaatttt tcactaaagt cgataaacgc
     2581 tacttccgta gggtcgccca atttagtacc gtcttcactg atcgatgcat cattcgctaa
     2641 aacactgatt tcgatcaagc gacgttcgtc agctgtccat tttgaagggt catttgtgaa
     2701 atccccagta tcgccattac ctaaaaagta gtcaacaact gtcattttgt tttgtgtcaa
     2761 tgtaccagtc ttatctgtac agatgatact tgtcgaacca agtgtttcaa ctgctggaag
     2821 cttccgaata atggcatgac gttttgccat tttattcgtt cccaaagata acacgattgt
     2881 tacgatagac tgtaatgctt ctggaatagc agctacggct acggctacgg caaacatcac
     2941 tgcgctgacg atatctgcag tcatatctcc tgtaccttct ccaagataaa tacgaaacag
     3001 ttggatacct aaaatcaata aagacaatgc caaaatagcc agacttagtt ttttactgaa
     3061 tttatccaga cctcgttgta acggagtgac ttgctcagtt gttgattcca aaaggccagc
     3121 aacttgtccg atctccgtat tattcccagt tgaagtcact aagaatttac cgcgtccgta
     3181 agttacgatc gttccactga ataccatatt tgtacgatct ccgattggta ccgtacttgt
     3241 gatatccgtt acttcttttt ctgcagaagt cgactctcct gtcagcatac cttcatctac
     3301 ttttaatgat cccgcatcta gtaaacgtcc atcagcaggt acataatcgc ctgcgtctaa
     3361 caacacgata tctcctacca caagttcgtt tgccggaatc gttttttcct taccatcacg
     3421 aatcactcga gcatcgggcg ctgaaaggtt tttcaacgct tctaaagaac cttctgcttt
     3481 ttttgtttgg acaacactga ttatagaatt caacatcaat actgcaaaaa taactaagaa
     3541 ttcgatgtag tcacccatga ccatctgaat cagtgcgaca atcagcaata cgataaccat
     3601 tgcatctttg aaagtttcta agaacaattt ccaagttggc ttcttcggct tttcttttag
     3661 ctggttcaag ccatctcttt ctaatttttc cttcgcttgc gtggtgctca atccatcggt
     3721 tgagctctgc gtttgctgca tcaacgagtc gatcgtttct ttatagaccg cttgttgtgc
     3781 ttgttccttg ttttccaaat ttcatccccc attttttgat cttggcccta gtcctgtctg
     3841 aaaaaaaaag agacctaaga caaatacgca tttgtcttag gtctcaccat ttcatacaat
     3901 accagaaaac catttggtta acttactgtt gatattatcg caacaaacgt tgccggttac
     3961 tcccctaagg agatattcaa ctgtactaat atcctacctt attaacctac aaaaagcaac
     4021 tactttagcc cgatttacct acattttcat gaaaggagtg aaaagtatat tcaccttggt
     4081 aggcttcttt cttctatcat gctatactaa tgttattgta tgtaaggagg aacatttttg
     4141 tggtatctag tattataacc gccctttatc tattaaatgc acttattgct ttggtggcta
     4201 ttttgatcaa accccgagat gtagcagcca tttgggcatg gctcttagta ttgtttgccc
     4261 ttcctggggt gggctttgta ttatatttat ttttcggacg tggattaacg gataagaaaa
     4321 aattctatct ccgacaaagt gacttgaaag aattagaaaa ctttcagtct tttagaggag
     4381 ataccattga acattacgat cctgacatgg gcgataaaga caaacagcaa tttgtcgact
     4441 tcttctcttc attaaatcgt atgccgctga caagaatgaa ttctgtcact cttctcacag
     4501 acggacaaga gaaattggat tcactgcttc aagatctaaa aaaagccaaa cattcgatcc
     4561 atatcgaata ttacgcattt gtgacagata atatcggcca gcaagtctta catgttttag
     4621 aagaaaaagc cgcagaaggc gtggaagttc gaatattata tgatgcattt ggctctcatg
     4681 gtacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg tggacatgtc catacatttg
     4741 ttacctcaca aaaggcatta cttcgtttcc gattgaatta ccatgatcac cgaaaaatcg
     4801 ttgtgatcga tggaaagatt agttataccg gtggtttcaa tattgccaat caatatgtaa
     4861 atacaacaaa aaaattcggc tattggcgcg atacgcatat acggattttc ggtgccgctt
     4921 cttctttgct ccagcttcgc ttcttaacag actggaacgt ctcggtacct gaagaaaaaa
     4981 aggtcggcta tcatttgaat tatttcttta aaaaagcaga tcgagatgaa tctaagcttg
     5041 ctgatacatc catccagctt gtttcaagcg gaccgaataa cgaaagggaa caaatcaagc
     5101 tttcatttat caaattgatt acttctgcta aaaaacgtgt ttggatacag acaccttacc
     5161 ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc aactgcctct ggtgtagatg
     5221 tgaaaatcat gattcccaac aaaccggatc atccttttat ttatcgagca acacaatatt
     5281 atgctcggca gctgatcaag gaaaatgtac aaatccttgt ctatgagaac ggcttcctcc
     5341 atgcaaaaac attgataatg gatgatgaaa tctgcatggt aggttcagca aatcaagata
     5401 ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat ttacgatcct gagtttttag
     5461 aagaacttgc tactcagttc aaagaagatg agacacattg ttcatccatg acaactgaaa
     5521 cagtcaagga aatgtctaac tggctattat tcaagcaaca aatttctcga ttattttctc
     5581 caatcctata atttttttat tcaagtagag aaaaatatac taagattaaa gggactgtga
     5641 caaaaatctt gtcacagtcc cttcttccga acaaacggtg ttctcctgcc aactcctcgg
     5701 tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaacttgt tccttgtgac
     5761 tcggagctgg acggcaggct cacgacctct ttcttttact ctaattcacc agctgcccaa
     5821 tgattgatgg gtccatattt gtgccctaca tttatttctt cacgaattgc acggtttaca
     5881 tagtcttttg ccaatcggat cgcttgttca actgtttttc ctttcgctaa ctcagctgca
     5941 atacatgcag atagcgaatc gccagttccg ttgatccggt cggtaggata aaatggtgca
     6001 tttaaccaaa atgattttcc tgattcgagc aatacgtagt cattgactgt ttcttgctgc
     6061 gcatctttag catgatgtcc tttgatcata acgttttttg cacccatatc ttgcagttct
     6121 tttgctgctt gcacaaaatc ttcttcagct gctaatgtcc gtccagtcag cttttctgct
     6181 tcataaaaat taggtgtcag tacttcggcc aaagggacaa gcttttcttt caagctttgc
     6241 aaagcagatt cttctagtaa aagatttcca tgtttcgtta cgattacggg atccacgact
     6301 aatggtccaa aatcatattt ttggtaattt tttacaactg tctcaatcaa ctttgaatct
     6361 gctagcatac ctgttttagc tgcacgtatt tgaaagtcct tcgcaatcag ttcaaattct
     6421 ttgtcgataa aatctgttgg caatgtcaca cttgctccga ttccgtaaga attacctgca
     6481 acacatgcgg tcatgacaga aacaccgtaa acatttcttg taaagaatgt atgcatatct
     6541 gcttgcatcc ctgcgcttcc atcgctatct gatcctgcga tcgtcattgc ttgtacatac
     6601 tgatcttcca tcaaatcgat tcctccattt ttataaaaag atttttcata atgctgttac
     6661 tgtttctatc atatgttttt caaagtatcc ctcagtaaat ataccactac cttttcttgg
     6721 taatcaaatt tatttgaaaa aacatttgta ataaatcaga aaatagttgt catttgtttg
     6781 tattcgctat tctaataata aataaccact atttgattta tatatatttc agtatccttc
     6841 ttgttttcat ataaccaaag gcgctatgat ttccatatga aaaaaataaa gtcaggtgaa
     6901 aaaaatgaaa cttgaggata aaaaagtcat tttgaagaaa atcaaacgac taccagacca
     6961 aatagatggc tatacgatct gttctttcaa aaaagacagg agtataaaag cttatacgca
     7021 aaacacagaa aaaggaataa tctatttcct acatgaagaa ggttacgata cccaaacctt
     7081 tatgtttact gaaaaaaaag aatttcataa acagatgaaa aaactgattg aaaaagaatt
     7141 tccaagaagt cataaactct atgtgaatca gcaattttaa cttctaactg ttcttta
//
LOCUS       DACDCJ010000111         7059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDCJ010000111 DACDCJ010000000
VERSION     DACDCJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.112"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            138..569
                     /locus_tag="IV038_002599"
                     /pseudo
     CDS             138..569
                     /locus_tag="IV038_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            734..961
                     /locus_tag="IV038_002600"
                     /pseudo
     CDS             734..961
                     /locus_tag="IV038_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1087..2058
                     /locus_tag="IV038_002601"
     CDS             1087..2058
                     /locus_tag="IV038_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8342760.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2198..3511
                     /locus_tag="IV038_002602"
     CDS             2198..3511
                     /locus_tag="IV038_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP8342761.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3554..4420
                     /locus_tag="IV038_002603"
     CDS             3554..4420
                     /locus_tag="IV038_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8342762.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4417..5265
                     /locus_tag="IV038_002604"
     CDS             4417..5265
                     /locus_tag="IV038_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8342763.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5279..6751
                     /locus_tag="IV038_002605"
     CDS             5279..6751
                     /locus_tag="IV038_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP8342764.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6768..>7059
                     /locus_tag="IV038_002606"
     CDS             6768..>7059
                     /locus_tag="IV038_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8342765.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEF"
BASE COUNT         2222 a         1189 c         1499 g         2149 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaattgtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac tagttttact gttatcatag
      121 catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt tgaatgaatg
      181 aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc tcggaacagt
      241 taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt agaaaaggca
      301 aactttatca aacttattac gacggtatgg aggaacagct caacgcatta ggattagtaa
      361 ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa caaattaaaa
      421 cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta attagtgatc
      481 acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg aatggaatac
      541 tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc taccgtgcat
      601 tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac acaagaatac
      661 taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt cgtttctatg
      721 ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca ttgacgactt
      781 agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg gtcctgttta
      841 ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg aaataaaatt
      901 gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg aatacgaaaa
      961 tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat tcctgctaat
     1021 ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag aggagttgtt
     1081 cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg tgtgggtacg
     1141 gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa agtaatggct
     1201 gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa aacgaatagt
     1261 agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc cgagtttgcc
     1321 tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg taatgcgaac
     1381 ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa agtagacgga
     1441 attataggta ttacctattc agacattgat aaatatgtat catcaaattt accatttgtc
     1501 agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa ttatttaggt
     1561 gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg ctatgttggg
     1621 ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta caatatttgt
     1681 gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag caatttagag
     1741 gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt taccatcaat
     1801 gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga agcacctaag
     1861 gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga ctatttacta
     1921 tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac actcatggcg
     1981 atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt tcacgctgga
     2041 gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag gttgacagaa
     2101 agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt aattgaaaaa
     2161 taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa tgttagcaat
     2221 tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta aggagaaaag
     2281 ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg atgaaacgcc
     2341 tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg agggtgaata
     2401 taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg aggataaagt
     2461 aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg gaccgaatac
     2521 tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga aagatgcgga
     2581 catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca aaatgtatgc
     2641 gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt tcaaagatgc
     2701 tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga cggaatttca
     2761 agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata tgcaaatggc
     2821 gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga atggcggcga
     2881 tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa aatccgttga
     2941 agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca gtcctattga
     3001 aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga caatggcaga
     3061 tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg tttcagatgc
     3121 aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg agaaaacaga
     3181 gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat ctagtcgtat
     3241 tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt taattaaaga
     3301 tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg gacatgcccg
     3361 cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag ttcaagatat
     3421 tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg ctgctgaaat
     3481 gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca atcaagtgtt
     3541 aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc cagctttgct
     3601 gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg cctttactga
     3661 ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt ttaagcgact
     3721 gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg ttgtttggat
     3781 tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca aaaatcgtaa
     3841 aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct tagtcgttat
     3901 ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata cgctatttgc
     3961 aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa tgtatacgct
     4021 tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt tagggggaat
     4081 gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt ctaagtttca
     4141 gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta ttttacttac
     4201 cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc aaggtggacc
     4261 aatgaattct actatgacaa tggtttatta catttaccaa actggattta cagaccgtat
     4321 ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt taattacaat
     4381 tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt tggaccgcta
     4441 atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt cccgatgctg
     4501 tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt agcttccttc
     4561 aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca agaggttatt
     4621 tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt aacctttgcc
     4681 tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt taatttttca
     4741 ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat ggagacggtt
     4801 ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg cttggcagtg
     4861 attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt ctttatcgcg
     4921 attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt taaaattttc
     4981 tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt cttatccttt
     5041 atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag cagcaaattt
     5101 tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat caatcaagtg
     5161 atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt tgcacaaaaa
     5221 tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg aatagtgaat
     5281 gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa cagtcaatca
     5341 agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg atccaaatgg
     5401 ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt acgacagcat
     5461 ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact gggagcattt
     5521 gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt ccggcagtgc
     5581 catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg tggaagggca
     5641 agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg aaaaatatgc
     5701 cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg aagatttccg
     5761 cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta ctaaaactgc
     5821 agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt gggagtttta
     5881 ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc cagacttgtt
     5941 tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc cacaaggcaa
     6001 tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt ggcaaacagg
     6061 caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt atgcacccca
     6121 gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa tgtggcagcg
     6181 taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct tgcctagaga
     6241 gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt atcaagtaat
     6301 tgatccgcag aaaaggattg aatgggcgaa gactgaagag aatcgcttct tactgaagga
     6361 cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg caacgaaact
     6421 agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg atgttgaacg
     6481 ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag ctgaaaagga
     6541 agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa ccatcgaaat
     6601 tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta tgagtacaac
     6661 tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg aagtaacctt
     6721 ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg atttatggtt
     6781 caatcgaagc aggcggcaca aaatttgtgt gtgccattgg cgatgaagaa atgacgatca
     6841 aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta gtcattgact
     6901 tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt gggcctatcg
     6961 atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa gtggcatggc
     7021 aaaattttga tttcatcggt accatgaaaa aagaatttc
//
LOCUS       DACDCJ010000112         6994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDCJ010000112 DACDCJ010000000
VERSION     DACDCJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.113"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            18..152
                     /locus_tag="IV038_002607"
     CDS             18..152
                     /locus_tag="IV038_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAP8342766.1"
                     /translation="MKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK
                     "
     gene            398..994
                     /locus_tag="IV038_002608"
     CDS             398..994
                     /locus_tag="IV038_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8342767.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDTERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1007..1906
                     /locus_tag="IV038_002609"
     CDS             1007..1906
                     /locus_tag="IV038_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8342768.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            1909..2393
                     /gene="ssb"
                     /locus_tag="IV038_002610"
                     /pseudo
     CDS             1909..2393
                     /gene="ssb"
                     /locus_tag="IV038_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2415..2672
                     /locus_tag="IV038_002611"
     CDS             2415..2672
                     /locus_tag="IV038_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342769.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2831..2965)
                     /locus_tag="IV038_002612"
     CDS             complement(2831..2965)
                     /locus_tag="IV038_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8342770.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            3108..3368
                     /locus_tag="IV038_002613"
     CDS             3108..3368
                     /locus_tag="IV038_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342771.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3817..4023
                     /locus_tag="IV038_002614"
     CDS             3817..4023
                     /locus_tag="IV038_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8342772.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            4023..4274
                     /locus_tag="IV038_002615"
     CDS             4023..4274
                     /locus_tag="IV038_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342773.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            4290..4727
                     /locus_tag="IV038_002616"
     CDS             4290..4727
                     /locus_tag="IV038_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342774.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            5408..5803
                     /locus_tag="IV038_002617"
     CDS             5408..5803
                     /locus_tag="IV038_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342775.1"
                     /translation="MKKKQTKLSLADILTQLTATEDGVVEFERSEISVVDDRYFKMPY
                     FFDQAKVICLCGYDGVRDYFGIRITEEKVVWVNNHTELGALAFEGTVLDNISIVFEEE
                     SFTLECDKLTRYIDPKFYEDKNLAWELAL"
     gene            5785..6051
                     /locus_tag="IV038_002618"
     CDS             5785..6051
                     /locus_tag="IV038_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342776.1"
                     /translation="MGTGFIDRVLVQVKEERKITMKYNLLDHKRMWVEKLEEDLEQLE
                     LLGHNKTSTVYKEGLKRTKKAQKELATLESRVQKKEVDLTHTYL"
     gene            6061..6294
                     /locus_tag="IV038_002619"
     CDS             6061..6294
                     /locus_tag="IV038_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAP8342777.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            6637..6819
                     /locus_tag="IV038_002620"
     CDS             6637..6819
                     /locus_tag="IV038_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP8342778.1"
                     /translation="MSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSEIVDRGVK
                     AYVNGFTKVLGGVGGR"
     gene            6809..>6994
                     /locus_tag="IV038_002621"
     CDS             6809..>6994
                     /locus_tag="IV038_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342779.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEER"
BASE COUNT         2540 a         1099 c         1427 g         1928 t
ORIGIN      
        1 gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa gggttcattt gaagcaacga
       61 ttgttttaaa agaagacttc actacaggct ttgaattttc tgaaaagaaa aaaacgaact
      121 tcaagaaaag aacacgccgc actgcaaaat aatggagggt taatcttatg cgtaatacca
      181 cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa
      241 tgcgtgccag caaacgaaga ctgcagcaat tgaagaacca gcagaggatt caaaaagaaa
      301 tcgaattaga tcgataaaat cttcctaact attgtatacg tataaactta tgttatactg
      361 tttttataag tttatacgta tacaaaggag ggagaaaatg acaaaagcaa tggtatacaa
      421 gctgctagaa gaatttaatg gaactttggc tttgaaggat gccaaaaaag cgggcatttc
      481 tcctgtaacg atcaaacgta tggtagatcg aggggagctg gatcgagagt atccaggttt
      541 tttcacctta cctggtcaat ttcctgatga attgtttatg gcgcaaaaaa aatatgaacg
      601 cggcattatt tcacatatta ccgcgttaga tttgtatgat ttaacagata tgatccctag
      661 acagatcgat ctgactgtac cttacggtta tcacgtttct gaaaaaggat tgaaggaatt
      721 cgcagttgag ctacactata caaaagcgga atggtatgaa ttaggtaaga ttgaaataaa
      781 aagtcgctat gggaatccta ttatagctta tgatacagaa agaactttat gtgacatttg
      841 gaatccctgg tataccgttg aggatgaaat caaagtaaaa gcaatcaaaa actatatgga
      901 gtcggacaga aaaaatctta gaaaattgaa tgagtatcga cgaattttac caacagacaa
      961 aacgatgcgt tcttatatta tggctttaaa ttaaaggaga agatacatga cacctacgca
     1021 gttaaaagca aaagtgaaga atatttctag agaaaaagaa gttgatccgc agcttgtttt
     1081 aagacatttt atgatggaaa aattcttgga aaaaatctca gagtctcctt atcgtgaaaa
     1141 ttttgtgtta aaaggcggct tcttgattgg ctcaaagtat ggcattgaaa atcgcacaac
     1201 caaagatatt gatacgactt tacgggaaat gaaagtaacc aaagaaacat taacaacggt
     1261 tctaaacgaa attttttcaa cgccgacgaa agaaggcatt caatttgaga ttcaaggaat
     1321 gaaagaaacg agagaagccg attactatcc aggatttagc ttacgtgtgc tggcacattt
     1381 ggaaaacatg cggccagatt ttaaggtaga tgtgactaca ggtgactcga tctatcctgc
     1441 aacgatcact cacagtcata aattgatgtt tgaggatcga acgattcctt tggaatccta
     1501 tcctacagaa caaattattg ctgaaaaatt aagtgcaacc tttgattttt taacagataa
     1561 ttcgagagga aaagattttt atgatctcta tactattcct aaaatggaga agatcgatga
     1621 gaagaccttg aaagatagtg tgagaaatac atttacacgt cgaggaaaaa ctgatccgtt
     1681 aaagatatac tatgaaaagg atatggatgt actgaaaaat agtcacgagc taaaagatac
     1741 ctggaagaaa tatcaaaaaa gcaatcctta tgctgcatcg atcacgtacg atgagacaat
     1801 ggattcgatt agtaatttga tggaaaaagt gattcacgtc gaagaaaaag agcaaaaact
     1861 agcttatcaa agacaccaac agatgaatag agaaatggag agataaagat gattaataat
     1921 gtgacattag taggacgctt aaccaaagat cctgatttac gttataccgc aagtggtact
     1981 gcagtagcga cttttaccct agccgtcaac cgaaatttca cgaatcaaaa cggcaatcga
     2041 gaagccgatt ttattaactg cgtgatttgg cgcaaaccag cagaaacaat ggcgacatta
     2101 gctaaaaaag gtagcttgat tggtgtagtt gggcggattc aaacgcggac ttacgacaac
     2161 cagcaaggac aacgtgtata tgtgactgaa gttgtagcag ataactttca actattagaa
     2221 aataaagcag cgacagagag ccgtgcgcac gctgatcaat cgtccaccag tccaagcacc
     2281 gccacctttg aacaacggat acagcgacgc ctaacaacaa tggattgaac gcttcacaga
     2341 atccatttgg tggacaatca atcgatatta gcgatgatga tctaccgttt tagtttgaga
     2401 aagaaggagt aagcatggca aaaaaaatta gtctgacgat ggatctattg atacccgtaa
     2461 ttgcatttat agacgggatt tctttgggcg tcactgaaaa tcctttggta aatagaattg
     2521 cgaaaggatc actatttctt gtattcgtgt atcttgtctg ttcttatctt ttaaaaagag
     2581 caggaaagga taattccttt tggcgattga ctaaagtcac agtagcagcg acagctgtta
     2641 tacttacggc tattctaccg ctttatatgt agagttaacg ggaaatcgtt gaacttcttg
     2701 cgaaatattg ctataatgaa catgaaaaag aggagttgcg ctaacaaccc ctcattaaca
     2761 gcaccgatta aagaacggtg gcagaatatt agttatttgg aaataaccgc tgacctgcta
     2821 aagtagacgg ttatttcttt ttgtcatttt taagcatatt tacaatcaaa ctaatcaaag
     2881 caatggtaaa cataccaaaa ccaagaatgg tttgaattgt ctcatatgcg gacaaaaggc
     2941 ttactccttt tatagatttt agcacttaca ttcataagca ccaccacctt tccagaagtc
     3001 gccaccgtct tcaaacttta ctgtcttgac tattatagca tatctaaaat cgaataaaaa
     3061 ttctcaattt taactgtttt tgctataatt ttagcgaggt gatcgtgatg aacaaatatg
     3121 ttggtttgct tgataaaatc cgagtaataa aaacacaacc attattggtt cgctttactt
     3181 tacaaacaat tcatgaatcg attaattgtg tagttgcaga tattgaaatt atcgataaat
     3241 tgttaattat ggatgatgga aaatacaata ttgccgtcac aggtcatttc aataaacgga
     3301 atcaattagt cattgaatcg atgcaaatta gaaaccctga tcattttacg cgatcaatgg
     3361 gcatataaat aaaagaagag ctgcaatcct aatgtaggac tgcagctctt tttgttttac
     3421 atagaaacag gaagaaaggg aaaggcctgt ttaggaaggc acaagtgcct tctcaattga
     3481 aaaacctttc tctcactcca accgccaccg ccgttcgaga agaaaggaag tgaacgttgc
     3541 acgttcacta agataagggg tcttgctttg taagacaaaa taggaaaagc ttcctctcac
     3601 caaaaggttc gttcctgttc gctatctctt tattgatttg ccagaccggc aacaagttgc
     3661 cttttatcac caaaaagcga caaggacaag ggtaaatggc acgctgcgcg cccttgtcct
     3721 tgtcgctttt cgttgattca ttctggccaa caaatcaata aagaaagaac taacgaaagg
     3781 aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc gaaagaaatt tgattatttg
     3841 ggtgttccct tcagcgaatt attcccaaac tatcatgcac cacatactgt cgaatgcgat
     3901 tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt atcaatgtcc aacatgcggg
     3961 aaaaaatata ccttgtctta tggcgattat attttgattg aggagaaagg aaagaaacgc
     4021 tgatgaaaac acaacacaaa ttattgactg taaaagaagt cgttcaaatt ttggtggaca
     4081 aagaagtcat ttctgaaaaa gtagcagaag ctttacttat tccgcggtac tggacgatag
     4141 aaaccccttt agtagaggat gcaccgacta tgctatttga agacctagtg aagattgcag
     4201 gatgtgaact gcattatcaa gaaaacttgg aaacttatga aaacgaatat tggttaacca
     4261 aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa ttactatgac gaatttgtca
     4321 aattaccttt ggataaaatg gcacaaaaaa tggaggatat gacgttccta tacaacgaaa
     4381 cacgtgttcc aaaaaaacat tataaagaaa aattgtcggt agctgtggaa gaaatgattg
     4441 aatcaggtgt cgaaatgaat cttattgcga cgtactatcg aacactggaa gaactgaaaa
     4501 agcaaaatgc aaaatggttt ttccaagcct tactatgctt tgaaataggt gtcaaaccaa
     4561 gcactattaa accttctgaa taccaggctt tagaactaac gtatgcgaaa tttatcgaaa
     4621 cgaaaaaagc caagactgtt tcaagtgaat ggttagatta ttttgaaaat atcaataagt
     4681 atggcgcata ttacaccatg aaaaaggaag acaacgaaaa tgaataaggt gatttatcaa
     4741 ctaacaacgg attccatatc aacgatttga aagaaaaaaa ggtcaataat catttatacc
     4801 gaaaagtgag aatgtgtgga ataaggacaa aaaaagccta gtaactaagg gaatggttct
     4861 tatttcgcac caaccgcccc cgccgcttag gtggtgcgaa ataaacgggc aaagcccgat
     4921 aacttaagag aaagaaggtg atccatttga aaaagattat tcccttattg ttagtactgt
     4981 ttgttggcga aatacttatg cgagatagtg cagctgtaca gttaaacctc gctgtatttt
     5041 tggtaatact ggtgttttgt ttgatctttt ggcggtttgt attagtcctc gtgagtctac
     5101 tattcaagat gattgcagcc atatttttca tggggttatt tcgatgaaag gagagagtac
     5161 caatgccaat tgcaatagga aataaacggt tacccgtgac attagatgaa aagagacaaa
     5221 aagaattgca gcaactaaag cagaagtacg gcaaaagtga atccaggatt atgtgtattg
     5281 cgttagattt attgattgcc aagaaaaagc aggatttgag gtaccagcac taagaaagtg
     5341 acgtcacttt ttatactaaa acctaaaagt gatatcactt ttagacaatg gaaaaggtgg
     5401 aaataagatg aagaaaaaac aaactaaact atcgttagca gatattttaa cacagttaac
     5461 cgcaactgaa gatggtgttg tagaatttga acgatctgaa atttcggtag tagatgatcg
     5521 ttactttaaa atgccttact tttttgacca agcaaaggtc atttgccttt gtggctacga
     5581 tggggtacgt gattactttg gaattagaat tactgaagaa aaagtcgttt gggtaaacaa
     5641 tcatacagaa ttaggtgcgc tagcctttga aggaactgta ttggacaata tctctattgt
     5701 attcgaggaa gaaagcttta cgcttgaatg cgataagtta acccgatata tcgatccaaa
     5761 attctatgag gataaaaacc tggcatggga actggcttta tagacagagt gctagtgcaa
     5821 gtaaaggaag aaaggaagat caccatgaaa tacaatttat tagaccataa gcgaatgtgg
     5881 gtagagaaat tagaggaaga cttagaacag ttggaattgt taggtcataa taagacttca
     5941 acggtgtata aagaaggact aaaacgaacg aaaaaagcac aaaaagaact agcaacactg
     6001 gaaagtaggg ttcagaaaaa agaagtagac ttaacacaca cttaccttta ggagtagaaa
     6061 atggcagaca agagaaaaga aaagattcaa aaattactgg cgcttgcggc ggatgtaaat
     6121 gatgaggaat cagtgagcgc attagccaaa gcccagcaat tgatgatgga ctatgatctt
     6181 actgaagatg agattttcaa ttatcatgag cagcaacgga tagaagcagt agaaacgcac
     6241 gtgatctatc gtggacgtcc gcaaaaatgg ctgtatcgtt tatttagaaa ttaagataaa
     6301 aaaggctagt taacaggttt gttgactggc cttttttgat atgtttgtac atgttttatt
     6361 gtgttttaat ttttctcttt tttatagcaa gctcctttta taataaatat tccgaaaagt
     6421 gaagaccatt ttttatttat taagactatt attattagtg agataaaatt tgattgatct
     6481 tacagaatat tggtgacgat tatgacacta aagatactat ttatgaaaat tttgcaaaaa
     6541 tgttctgaaa attacatata attgttttat aaaaaaggga ggcaattata tgaagaaaaa
     6601 atttgttagt attttttgat tttaggaatt gttttattga gtgtatctac tttaggaatt
     6661 acagtagatg ctgcaactta ttatggaaat ggtgtttatt gtaatactca aaaatgttgg
     6721 gtagattgga gtagagctcg ctctgagatt gttgatagag gagtaaaagc ttacgttaat
     6781 ggttttacaa aagtgctagg tggagtcggt ggaagataaa aaagaaaaac tttttgactt
     6841 aattgcgaat gcatataatt caaagtcata taataatgta gaagtaaaag aaggatttct
     6901 tcaagatgct aagaaaatac gagaggataa accatatcct gaaattatta tatcagtgta
     6961 caaacattta tctaaattaa aagctgagga acgc
//
LOCUS       DACDCJ010000113         6775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDCJ010000113 DACDCJ010000000
VERSION     DACDCJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.114"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(416..1123)
                     /locus_tag="IV038_002622"
     CDS             complement(416..1123)
                     /locus_tag="IV038_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342780.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1116..1553)
                     /locus_tag="IV038_002623"
     CDS             complement(1116..1553)
                     /locus_tag="IV038_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342781.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1568..1819)
                     /locus_tag="IV038_002624"
     CDS             complement(1568..1819)
                     /locus_tag="IV038_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342782.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(1819..2025)
                     /locus_tag="IV038_002625"
     CDS             complement(1819..2025)
                     /locus_tag="IV038_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP8342783.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            complement(2465..2725)
                     /locus_tag="IV038_002626"
     CDS             complement(2465..2725)
                     /locus_tag="IV038_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342784.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRSMGI"
     gene            2800..3003
                     /locus_tag="IV038_002627"
     CDS             2800..3003
                     /locus_tag="IV038_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP8342785.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3144..3629)
                     /gene="ssb"
                     /locus_tag="IV038_002628"
     CDS             complement(3144..3629)
                     /gene="ssb"
                     /locus_tag="IV038_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8342786.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3632..4531)
                     /locus_tag="IV038_002629"
     CDS             complement(3632..4531)
                     /locus_tag="IV038_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP8342787.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFADTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4544..5137)
                     /locus_tag="IV038_002630"
     CDS             complement(4544..5137)
                     /locus_tag="IV038_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP8342788.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5377..>6775)
                     /locus_tag="IV038_002631"
     CDS             complement(5377..>6775)
                     /locus_tag="IV038_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP8342789.1"
                     /translation="GTIHQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAIQ
                     GLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKK
                     VQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQ
                     LQLKAIGKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQ
                     PYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNK
                     LVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLL
                     EVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACD
                     FKLWTTIAKKKLTATQLKEIIQNGRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSE
                     KKKTNFKKRTRRTTK"
BASE COUNT         1739 a         1393 c         1161 g         2482 t
ORIGIN      
        1 acgggtaacc gtttatttcc tattgcaatt ggcattagta ctctctcctt tcatcgaaat
       61 aaccctgtga aaaatatggc tgcaatcaac ttgaatatta gactcacaag ggttaacaca
      121 aagcgccaaa agatcaaaca aaataccagt attcctaaga atactacgaa gtttaaatgt
      181 agagctgcac catgacgcat aagtatctcg ccaacaaaca gtacgaacaa tagaggaatg
      241 attttattca tatggatctc cttctttctc ttaggttatc gggctttgcc cgcttatttc
      301 gcaccaccta agcggcgggg gcggttggag cgattggtgc gaaataagaa ccagtccctt
      361 aatttcaagg ttttttgttc ttatttcaca catcctcact tttcggtata aatggttatt
      421 gacctttttt cctttcaaat cgttgatatg gcattcgttt acggtcaagt tcattttgga
      481 tttcttctgt atcgtcgtaa gtgtaggtgt ctaaaaaatc agttaaagtc gtgtaccagt
      541 cgtcgcctgg gttatctact tcttctacat attccctcaa aaatgcttcg ggtacagcga
      601 gttcataatc catccccatt aacacgatag attccgtccc acacgtcaaa aatctagtaa
      661 atacaaggta ataatcgtct accgtcgcat ctttgctgtt tagctcttcc atttcttctt
      721 ccattaattc catgctctca aaaattgaat aatattctga ttctgagtaa tagtgtttcc
      781 tgcaaatcgt gtagctgaca aagttttcga ccagctcact cctttctagg taaacattca
      841 tgatatattt ttctgtttct agtcgtagaa gctccgctcc tttttttaat gtgtcaggac
      901 gacgtttata cacgtctaaa cgattatggc tgactgtaac cttcgtttga ctcgtttcac
      961 tttccatata caactgttct tccactcttt gaataaagta cttcatttct tccatacaac
     1021 taaagtcttt ttgaatccgt ctgattactt cattcaagtg ttctttgttt gttaaaaatt
     1081 gcgaaacatc ttctgttgtt agctgataaa ccaccttatt cattatcttt ctcctccgat
     1141 tcaatcgtat aaaatgcacc atttttttcg atattttcaa aagtatctag ccagtcgcta
     1201 gccattgctt tagctttctt aatctgtgcg aattttgcat aagtcagttc taaggcttga
     1261 tattcggaag gtttgatagt gcttggtttg acacctactt ccaagcatag taaggcttgg
     1321 aaaaaccatt ttccattttg ttttttcagt tcttccagtg ttcgatagta cgtcgcaatg
     1381 agattcattt cgacacctga ttcaatcatt tcttccactg cgactgacaa tttttccttg
     1441 taatgttttt tgggtacacg tgtttcatgg tataggaacg tcatatcctc cattttttgt
     1501 gccattttat ccaaaggtaa tttgacaaat tcgtcatagt aattttgagc catatttttt
     1561 ctccttttta gttctctaat ttggttaacc aatattcgat ttcataagtt tccatgtttt
     1621 cttgataatg cagttcatat cctgcaatct tcactaagtc ttcaaatagc atagtcggtg
     1681 catcctctac taaaggggtt tctatcgtcc agtaccgcgg aataagtaaa gcttctgcta
     1741 ctttttcaga aatgacttct ttgtccacca aaatttgaac gacttctttt acagtcaata
     1801 atttgtgttg tgttttcatc agcgtttctt tcctttctca tcaatcaaaa tataatcgcc
     1861 ataagacaag gtatattttt tcccgcatgt tggacattga tacaaacgat agcttttgat
     1921 acatttcgct ctttctccac aatcgcattc gacagtatgt ggtgcatgat agtttgggaa
     1981 taattcgctg aagggaacac cccaataatc aaatttcttt cgcatgattg attctgactc
     2041 ctcttaggaa agggtttact tcctttcgtt agttctttct ttattgattt gttggtcaga
     2101 atgaatcaac gaaaagcgac aaggacaagg gcgcgcagcg tgccatttac ccttgtcctt
     2161 gtcgcttttt ggtgataaaa ggcaacttgt tgccggtctg acaaatcaat aaagagataa
     2221 cgaacaggaa cgaacctttt ggtgagagga agttttttct attttgtctt gctttgcaag
     2281 acaccatatc ttagtgaacg tgcagcgttc acttcctttc ttctcgaacg gcggtggcgg
     2341 ttggagtgag agaaaggttt ttcaattgag aaggcacttg tgtcttccta aaaaggtctt
     2401 ttcctgtttc tatgtaaaac aaaaagagct gcagtcctac attaggattg caactcttct
     2461 ttttttatat gcccatcgat cgtgtaaact gatcgggatt tctaacatac atgctagcaa
     2521 taactagctg atttcgttta ttgaaatgac ctgtgacggc aatattgtat tttccatcat
     2581 ccataattaa caatttatcg ataatttcaa tatctgcaac tacacaatta atcgattcat
     2641 gaattgtttg taaagtaaag cgaaccaata atggttgtgt ttttattact cggattttat
     2701 caagcaaacc aacatatttg ttcatcacga tcacctcgct aaaattatag caaaaacagc
     2761 taaaattgag aatttttatt cgattttaga tatgctataa tgatcaagac agtaaagttt
     2821 gtgacggtgg ctatccgatt gaaagtgagg tggtgcgtat gtgcgtactt ccaaaatctt
     2881 acagaaagga gaagcctttt gcctgttgaa acggcgctag gattaatgat tagtttcggt
     2941 tctttgatcg caacaatcat ttttggaatc ttagcagttg taaacaacaa tcaaaaaaaa
     3001 taaccgtcag ctttagccgg tcgacgatta tttttcaaaa ataatattaa gctggtcacc
     3061 gtcttaaacg gtgctgttag gggggttgtt agcgcaactc ctcttttcgt atccattata
     3121 acaactttat tgatctttta caatcagaat ggtaaatcat catcactaat atcgattgat
     3181 tgtccgccaa atggattttg tgaagcgttc aatccattgt tgttaggcgt agctacatcc
     3241 cgttgttcaa aggtggtagg gcttggactg ctggacgatt gatcagcgtg cgcacggctt
     3301 tctgtcgctg ctttgctttc taatagttgg aaattatcgg ccacaacttc tgtcacatac
     3361 acacgttgtc cttgctggtt gtcataagtc cgcgtttgaa tccgcccaac tacaccaatc
     3421 aagctacctt ttttagctaa tgtcgccatt gtttctgctg gtttgcgcca aatcacacag
     3481 ttaataaaat cagcttctcg attgccgttt tgattcgtga aattacgatt aacggctaag
     3541 gtaaaagtgg ccactgctgt gccacttgcg gtataacgta aatctggatc tttggttaac
     3601 cgtcctacta aagttacatt attaatcatc tttatctctc catttcttga ttcatttgtt
     3661 gtgatctttt aaactgcctt tttctttctt gattttcaat tttgatcaca cttttcatta
     3721 ggtgatcaat ttcgttgact gtatctgcaa atgaaatgga attcgcaaac gtgttaactg
     3781 agcgatattt ttcccactgc ttttccaaat agtcgtttcc cctgatctta tccatttctc
     3841 tttcataata aaaggataaa ggctttgtat ctccacgttt tgtaaatgta tttcgaacac
     3901 ttttgtataa ctctttcgta tcaatatttt ctagtttcgg aatggtaaat aagtcgtagt
     3961 aatcttttgc tctcgaatta tctgtgccaa agctgaacgt agcacttagc ttttctgcaa
     4021 taatttgtgc tgttggataa gctggaatct ccactttttt atcttcaaac attaattggt
     4081 gatagtgtgt atctacttca ggataaatag aatctcctgt tgtcacatcc attttaaacg
     4141 gcacacgtac tttctctaaa ttagccacca atttaatttg aaatccagga taaaaatcgg
     4201 cttctcttgt ttccttcatt ccttgaatag aaaaaacaat gccttctttc gtgggtttcc
     4261 tagttaaatc attaaaaata ctcttcaatt tttcttcagt taatttgcta tttctaaatg
     4321 tcgtatcgat atcaattgtt gtccttctat cgattccata ttttgatcct aataaaaatc
     4381 cacctttcaa aatgaagtca tttttgtact ttgattcaga aatttgttca attaaacgat
     4441 ccatcataaa atgccgttgc actaactgtg catttaatcc tgtactcttt gctaaatttc
     4501 ttgtttttgc tgcaaattgt gtcggtgtca tctttttcct ccatcaatat aagctttgca
     4561 tgtggtattt catttctttt tctacatgta gttttgtcat gtaatctctt aatttacttg
     4621 gatctctcaa tgggtcttcc atgtactctt ttaatgcttc taatttcatt tcatattcaa
     4681 tttcatatct cggattccaa atatcacaaa gtgtacgctc tttatcatac atagtaatag
     4741 gatttccttg aaaggaagtt ccctctactt ttcctagttc ataaacttct ttcttcacga
     4801 aatgaaattg taactgctgc tcttttgtaa ttgaacgatc aacgttatag ccatggggaa
     4861 aagttaagtg aatgaatttc ggcatattcg tagaaagctg gtaaatatct aaagtcgttt
     4921 catgagagaa gattcccctg gaaaatcgtt gctgaagcca aaataaatca tctattccat
     4981 gaccatcctc tacataataa ccacgatcaa ctttgatcaa ttttccttga ttcaccaatc
     5041 tttgtacagt ggttgctgcg attcctaatt ttctggcttc cttcatagat aaatttccat
     5101 cgcgaatagc caacaattct ttcagtttct cttgcattta ctcccccctt ttcgttacaa
     5161 atacactaca ttaaatatat catgatagtg tatttgtaac aaggttaatg tctatcgatc
     5221 taattcaatt tctttttgaa tccgctgctg gttctttaat tgctgcagtc ttcgtttgct
     5281 ggcacgcatt ttttcctgtt ctgaacgaat tttggctttt tgattttcga tttcttgttg
     5341 tttgtgtgtt gtattacgca taagattaac cctccattat tttgtagtgc gtcgtgttct
     5401 tttcttgaag ttcgtttttt tcttttcaga aaattcaaag cctgtagtga agtcttcttt
     5461 taaaacgatc gttgcttcaa acgaaccctt ctgacctttt aaccctttca ctggttggct
     5521 tgttcggcca ttttgaatga tctccttcag ctgtgtcgct gtgagtttct ttttggcaat
     5581 cgttgtccat aatttaaaat cacaggcttc agacgtacaa gctgccacct tttgatagag
     5641 caagacttct tgttcctggc attttggaca aattcctaaa tgacgcgcgg cttcttgctt
     5701 ttcactggct gctttcactt cttgtaacgt cgatccaaaa tcagtggatt ggatttgccc
     5761 tggtacgact tccaataagt gagagacaaa tttttggata ttggctagaa aagtggtttg
     5821 tgttcctttt cgttcaccga tctttaacaa gtagctttcc catttggcgg tcatttcggc
     5881 gctcgtaagc agcggctctt tttccacggc ttggcaaagc aaaatccctt tattcgtcac
     5941 cacgagctta tttttctcta ccttgatata ttccttttgt ttcaaggttt caataatatt
     6001 cgcccgtgtc gcttctgtac cgattccttc cacttctttc aatatggatt gcgcttcttc
     6061 actatccacg gttttcccag ctgttttcat tgcagtgatc aacgtacctt cggtataagg
     6121 ttgtggtggc ttggtttctt tttcaagaac ttgtagatcc accgtcacca tttctccttt
     6181 tgtcacttta ggaagtagcg gctcttcttc cttctctttt ccgtcgctct cttttccaaa
     6241 taacttcttc caacctagat ctagcggcac ctttccgatt gcctttaact gaagctgctg
     6301 aacttgcgtc aagatcgttg tttcttcata ggtatatttt tccgcaaaca tggccaccgt
     6361 tgtttttaac acttgttcat aaatggcctg ctgcagtgga cttaattgtt caaaagccgc
     6421 tgccgtcatg accgtttttg taggaatgat cgcgtaatgt tcctggactt tcttattatt
     6481 cacatatcga ctattcggtg tatgaatagg agtaggaatg gcttcggcct ttaaaaagtg
     6541 tttgtattcg tctaagtgat ccaacagata cgcatactcc ccttctgtaa tataaggggt
     6601 atctgttcgt gggtagctta gatatttccc ttcatacaac ccttgtatgg cttccaaagt
     6661 atctttggca ctagctttca tgagctgatt cattttagac tgaaggctgg atagagaaaa
     6721 aagacgtgga cttgccgcac tttttttctc ggtttcaact tggtggatcg tacct
//
LOCUS       DACDCJ010000114         6703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDCJ010000114 DACDCJ010000000
VERSION     DACDCJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.115"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(46..459)
                     /locus_tag="IV038_002632"
     CDS             complement(46..459)
                     /locus_tag="IV038_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP8342790.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(915..1400)
                     /locus_tag="IV038_002633"
     CDS             complement(915..1400)
                     /locus_tag="IV038_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342791.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1539..1754)
                     /locus_tag="IV038_002634"
     CDS             complement(1539..1754)
                     /locus_tag="IV038_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP8342792.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1978..2778
                     /locus_tag="IV038_002635"
     CDS             1978..2778
                     /locus_tag="IV038_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP8342793.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2797..4665
                     /locus_tag="IV038_002636"
     CDS             2797..4665
                     /locus_tag="IV038_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8342794.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4658..5149
                     /locus_tag="IV038_002637"
     CDS             4658..5149
                     /locus_tag="IV038_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP8342795.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5137..5691
                     /gene="srlA"
                     /locus_tag="IV038_002638"
     CDS             5137..5691
                     /gene="srlA"
                     /locus_tag="IV038_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP8342796.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5709..>6703
                     /locus_tag="IV038_002639"
     CDS             5709..>6703
                     /locus_tag="IV038_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP8342797.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2335 a         1072 c         1317 g         1979 t
ORIGIN      
        1 caaatatata aaaatcgccc ctcattcatc gagaggcgat aagttctaat ttaactcaat
       61 tttttttgtt tcttttggct gataagctgt tttaggtaat accaatttca gcacaccgtt
      121 tttaaaggtt ccagtgattt tttcttcatc aacattagga agatagaatg aacgtttgta
      181 agagaccgaa tgtctttctt tccgtacata gttgccatct tctttatctt ctgtatggga
      241 ctcatgattg gcggagatcg tcaaagtatt atctgaatat tcaactgtcg tgtcttcttt
      301 gtcacagcca ggcatatcag cagttagttt gtattctttt tcattttcga ctaaatctac
      361 ttttggataa cttgaaacac ctaaaaaatc attgaatgct ggactaaaca cgtcattgaa
      421 atcagggaac atgtcgcgaa tgctaggtac atttgccatt tttgatcatc ctttcaataa
      481 gtatttttta tttatcccta tttacgtaat aactctattt tgaaaaaaat gcaagtataa
      541 tggttagctt tgtgaatatt tcatctaata cgatacaaag gctatctttc taatttgaaa
      601 aagggatcat taaaataata agaagaaaat atgtcaaaag gtaaaaaagt aaattcttct
      661 atccaaatat agaaaaattg aatctagaaa accgcccgac aataaatttc attaactcag
      721 ttgaccgaaa atgaagtaga taccaaaaat ttatcaagag agcaagtaga gaagtgagta
      781 tattcataag agaaatagct gtaacgaatt attaggttaa tgcaaatgct tatttatact
      841 ccgtataact ttctcagaaa aaatgatacc aataatgact tgctagtact gtaaatccgt
      901 ataatatcat ctaatcaaaa tggtgttcca tttgcatctg tcatcgtaat cgatccttcg
      961 ggtgacactc gataaaaacc aacgcttcca gatcctcctt gggaacggat ttgtttgctg
     1021 gaagctgtga acaaataatc tcgatcaatt ttttgataaa agctaataac aatatcgtca
     1081 ctttgtaatt cggaacggtg ggcaataaga aatagaagtg ctttgtcaga gttttctaat
     1141 ggaagagaag gtacagtgct ttcttgcatt tcagatgttg aagaaatgct aaacgactga
     1201 atacttgatt ggctatgttg cgaatctaca ttaccagtag aagtattagt aatggaagta
     1261 gtagaagtac ttttagttga accaacctca gttgttgaaa tggctgctga agattcttta
     1321 acagaattct ttgttttata tccacaggca gaaaaaagta ttacagttcc caacaagcct
     1381 gcgtaaatcc ctttatgcat gatagctcct cctaaaatag attatcttta ttcagtatat
     1441 cacttaaaca aagaaaagca gatggtttaa aatgaaaaaa ccttcccaat aaagggaagg
     1501 ttcatgaaga taataacttt tttaataatt atagctttct aatatctttc atcaactttt
     1561 ctagtaatca ttgggaaaaa tttcaagcag tagatcgcac aaatacctac gacgatataa
     1621 atgatcttcg caaaaatagt tgttgaacca ccagcaatca tagctactaa atcaagttcg
     1681 aacaagccta ccaacagcca gttcaaccct cccacaatca ataaagttaa tgcaatagca
     1741 tctaaagttt tcattagaat tctcctttct taaatgataa aacaattata tatcaaaaag
     1801 agaaacattg cttatataac gctcaggaat tttagcaatc tatgcaaaaa gatgaaaatt
     1861 ttttttgctc ttccattagt acacattttt tagctgaagc tgtaaatttc atgtcttaat
     1921 tttttttagt gagcttttat actggatttg taagaaaaaa gaaggagagg atcacatatg
     1981 tcggattggt tggatattgc aggaaaagta gtaattgtaa ctggaggttc ctcaggaatt
     2041 ggaagaagta tcgtcgaaaa tttgttgaag cagaatgcac aagtagcgaa ttttgatgta
     2101 actgaatgcc gtatacagca tgaaaaccta ctatctttaa aagtcgatgt gtcttctaaa
     2161 acggacatag aagaagggat atataaagta atgaaacatt tcaagacgat cgatggtcta
     2221 gtcaataatg caggaataaa tattccttct cttctaatag atagaaatca tccaaaaagc
     2281 aagtatgaat tatcagaaca agtttttgat aagatgatcg ctgtcaatca aaaaagtgta
     2341 tatttgatga gccaagcagt tggaagaatc ttggtgcaaa agggatcagg agtgatcgtg
     2401 aatctttctt ctgaatcagg attggaagga tcagaaggac agagctgtta tgctgctaca
     2461 aaagcagcga tgaacagttt tactcgttct tgggcaaaag agttaggaaa gaggaatgtt
     2521 cgtgtggtag gtgttgcacc agggatactg gaagaaaccg gtttgagaac acaagaatat
     2581 gaagaagctc tttcttatac gagaggtata agcgtcgaac agctgagaaa cggttacagt
     2641 cgaacaagta cgatccctct aggaaggagt ggtaagctgc aagaagtggc ggatctagtc
     2701 tgctattatc tttcagaaag atcaagttat atcactggag tcacgactaa tatttcagga
     2761 ggtaaaacaa gaggataaag gatagggggg acgcacgtga cgctagtaaa cagatggtac
     2821 caattattga aattactagt cactcacaag aaagtttcaa tccaggaagt gcaaaaagaa
     2881 atcaaaacca gtaatcaaac cataaaaaaa gatattgggg agttgaatga agaaattgcc
     2941 ggtattgcaa aaatcatcca aaaaaacaaa cattttgaac ttgaaattta tgattttgat
     3001 ttttttgatg agatcatgca aggaaaattg aaaacagcca gtgatttcaa ttcggcatcc
     3061 aaaagaatcg cttatttact aaataggctg atcgaaacag aggatttctt acgtatggat
     3121 gatttgtctg aagaagtcgg tgtcagcaga ggaacagtgg tcaaggatct aaagacatta
     3181 aaagagatga tcaatgattt tgatgtaaag attacaggca caccaaataa aggactaaga
     3241 ataataggag atgagctaga attacgacta cttttatttt attttgtttc tccctacttt
     3301 tcagagacga aaacagaaca gtttctagaa gaaaaacaag caatcaaaca ttttcagaag
     3361 aagacgaatg ctagtaaaca agaaatcacc cttttaacga aagttatcgg tatttctttg
     3421 aatagaatct ctagtaacta tctgttgaag aagcccatca atcgttacag tgggtgtttt
     3481 gaatatatcc aagaattcaa tgaactgatc aatcagttgg aagaaatcta tgaactaact
     3541 ttgagccaat atgaaaaaga ttttatcagt tttcctttga acatcatcaa cacaagtata
     3601 acgatgataa ggaaagctaa ctctgatttg cgcttatatt tcgaccccat gatgatgcgt
     3661 attcgacgat tagatctcat tgatctggat gagtccttct tatatcaaga gatgaaacag
     3721 cacctctctt tattgataaa tcgggtaatt tttcgttatc gattgactga tttattttat
     3781 ggtgagatag aaaaaaagta tcctttttct taccaaatcg ctagcgaatg catacaagca
     3841 ttagaagcta tcttgcaaca taagatctct aaagtagaga tcagctacct aacgttgtat
     3901 ttggagttag catttcgata tcaaaaagag catttttcct ctaaaaaagt agccattgtt
     3961 catacgacag gaaaaggaac agcattaatg atccaaagac aaatcaaaag gatattgggg
     4021 agggatatac aaataacaca tgtttcagaa gaacaatatg aaaaagtaga tctaaacaaa
     4081 tatttcgctg tttttacaac gattccgtta aaaaatgtaa atgaagagac accggtgatc
     4141 catttgacta atctttttga tgactcttgg ttaagggcac aatggcaaaa agcaaaaaaa
     4201 aagcgattag ccgggtttca ttctactgtt tttttctatc aaatatggaa agaaaaaaag
     4261 acgtatcaag aatatttaaa caaattagtc gctcctttga tcagaggcta ttatgtggat
     4321 gaagaatttg aaaaaaggat cttgaaaaga gaatatcagc aaagcacatt tgttaacaaa
     4381 gaatttgctt ttcctcacac aaccaaccag ctatctcaac acattgtttt atcagtaggg
     4441 ctaataactg atcaaggttt agcagaaaca ggaggaaatg gagttatttt tttatttgcc
     4501 atacctgaag aaatgacggc tgaagttgag aatgaattat ttgagctata cgatatccta
     4561 tttgaactca taaaaatggg aatagatgag tttattgaat tagcaccgac acaagaatca
     4621 tttttaaaac taatcaaaag taaaggagag agacttcatg aatgatatgt atctattcat
     4681 agttttcgca gcttcagctt atgtgctgca agcagttatc agttggaagc aactaaagca
     4741 tttcaatcaa acatatagcg aactgcgaag aaaaggaaaa gtagcgattg gtaaacgacc
     4801 tgggaaaatc cgttcaggca cgattgttat gctagctata gacgatcatg atcaagtttt
     4861 agaagtcaga aaaatgcaag gggttacatc tttggcaaaa tttcattcat tacctgtcta
     4921 tgcgggtgaa gatatccatt acatcgatcg ttaccatccc tcagttcgag cggaagatcg
     4981 tttgacacaa gcagctttag aaaatgccag agaagtatac ttacgaatag aggcaggaaa
     5041 tgaaaaaagt gaacaacctt cattgccttt tattgctatg ctcaataatt ttcggatatg
     5101 gaaaagtacg atcaaagaaa aaaggagtga caaacgatgg atatcataac aaaatttgct
     5161 gagggattta tgaggctgtt ccaaacaggt gcagaaacat tcataagctg gatgacagga
     5221 atcgtaccag ttgtcttgat gctgatggtt gcaatgaata cgctgattgc ttttttaggt
     5281 gaagaccgag taacgaaagt agccaaatta tcagcgaaaa atccgcttac tcgttacctt
     5341 gtgttaccgt tcttatctgc ttttatgttg ggaaatccta tgtcatttac aatggctcgt
     5401 ttccttccag aatactacaa accaagctat tatgctgctc aagctcaatt ttgtcatacg
     5461 agcaatggtg tattccccca tattaatcct ggagaattgt ttgtatggat ggggattgct
     5521 caagggatcg aaaccttagg gctaaattcg atggagctgg ctatccgata cttgcttgtg
     5581 ggactgctga tgaactttat tggtggttgg ataactgatt ttactactgg atttgtatgc
     5641 agacagcaag gaatcgttct tagcaaaaaa gtagagcttt cagttgatta gagaaaggag
     5701 agttttaaat gtatggtgat attaagatcg taagaggaaa cggcgggtat ggaggtcctt
     5761 tgattattag gccgacaaaa gataaacaca agttcatcta tgtcacaggt ggaggtgaga
     5821 ggccaatgat cgtagataaa atagaagaac tgacaggtat ggagtctatc aatgggttca
     5881 aaacatcaat acctgatagt gaaatcgcgc ttgcaatcgt tgactgtgga gggactctta
     5941 gatgtggaat ttatcctaaa aaagggatac caaccatcaa tatcgtttcc acaggaaaaa
     6001 gtggcccatt agcacaatat atcactgaag atatttatgt ttcagcagta gggttagaac
     6061 aaatacagcc actaatggaa actgatgata acaaagaaac agtaaatact gcatctccag
     6121 ataaaaagta caaatatgat acttctaaaa agataaccga gcaaaaagca gagcagcaaa
     6181 actggatagc aaaaattgga atgggcgcgg gaaaagtagt tgctacattc aatcaatcag
     6241 caagagaagc agtagatacg atgatccgga cagtgatccc ttttatggct ttcgtttccc
     6301 ttttgattgg tgtgatacaa ggttctggga tcggtaatgt atttgctaac attatggcac
     6361 ctttagcagg aaatgcagtt ggattaacaa tcattggctt tatttgttca ctgccatttt
     6421 tatctccttt gcttggtcca ggggcagtta ttagccaagt gattggaaca ttgatcggtg
     6481 tagagattgg gaaaggaaat attcctcccc aattagcatt accggcacta tttgctatca
     6541 atactcaaaa tgcatgtgat tttattccag tcggtctagg tttggaagaa gcaaaagctg
     6601 agacagtaga agtaggtgta ccatcagttc tttattcaag atttcttaac ggcatcccac
     6661 gagttatcat tgcttgggta gcaagttttg gattatatag ctg
//
LOCUS       DACDCJ010000115         6612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDCJ010000115 DACDCJ010000000
VERSION     DACDCJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.116"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..284
                     /locus_tag="IV038_002640"
     CDS             <1..284
                     /locus_tag="IV038_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342798.1"
                     /translation="AMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIF
                     ITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(410..1087)
                     /gene="nth"
                     /locus_tag="IV038_002641"
     CDS             complement(410..1087)
                     /gene="nth"
                     /locus_tag="IV038_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP8342799.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1094..1792)
                     /locus_tag="IV038_002642"
     CDS             complement(1094..1792)
                     /locus_tag="IV038_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP8342800.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2060..3244
                     /locus_tag="IV038_002643"
     CDS             2060..3244
                     /locus_tag="IV038_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP8342801.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3250..3693)
                     /locus_tag="IV038_002644"
     CDS             complement(3250..3693)
                     /locus_tag="IV038_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP8342802.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3919..4206)
                     /locus_tag="IV038_002645"
     CDS             complement(3919..4206)
                     /locus_tag="IV038_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342803.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4377..5729)
                     /locus_tag="IV038_002646"
     CDS             complement(4377..5729)
                     /locus_tag="IV038_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP8342804.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6002..6448)
                     /locus_tag="IV038_002647"
     CDS             complement(6002..6448)
                     /locus_tag="IV038_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP8342805.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1791 a         1310 c         1085 g         2426 t
ORIGIN      
        1 atgcaatgat tctcactttt ttatggttgt tgttcaccag aatctatatt tttctgatga
       61 gcctgaaacc gcccaaatcc attagtttat gtttagttat aaatggtatt tttttattat
      121 cacttatcat ttttattaca cctttagata gatacgtcac ttatttatat aatcctttgg
      181 aatactggac ttattttata ggtatattgg aaggcattat tttctacatc gggtattttc
      241 ccaataaaaa taacaacttt tatttttatc gcaataaatt gtaataagtt accatcctag
      301 ctactgagta tgtacattta ctttttcatg caaaaaagat aaacgaaaca gcttttctcc
      361 ataataacaa gataattcat acatttattt gcaattaaag aaaccacgtc tacaggcgtg
      421 gtttcttttt tagcgccttt tcttttagac gcatccgatt tttcccatct tgacatattg
      481 atagcaaagg acatacctcg cacttgggat ttcttgccgt gcaatgatat ctaccaaaaa
      541 aaatcagtgt atgatgtgtc tttacccata attcttgtgg tactttccgc atcagtgttt
      601 cttctacttc catgaccgta gcatctagtt tgcagatcct caggcgtttt gagacacgtt
      661 ccacgtgggt atctactgcg attgctggta tcccgaatgc atcccccaat acgacatttg
      721 ctgtctttct gcctacacca ggaagagaca tcaattcttc tcttgacgtt ggcacttgcc
      781 catcgaaacg ttcaatcagc tgctgtgcac acgctttgat gttttttgct ttatttcgat
      841 acaaaccaat cgtttttatt ttaagaatga tttcatcgat tgaagcttct gctaacgcat
      901 cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt attcactgat acatctgtcg
      961 cctgggcact taagatcact gcaatcaata actcaaatgg attattgtgc ttgagttctc
     1021 catgtgcttc gggaaacatt ccatacatcg tttctagagc ttccattgtt ttttgtttgc
     1081 ttaacatatt ccgctactcc tccaaccaat ttttcagtga gactttcggc aattcttctt
     1141 gttgagctga ctgttctact tctctttgaa gaagctgctg ttttcttcgt ttttggtctt
     1201 cttccacttg ttgttttgta cgtagatttt ttctttccca cgatagcaaa atgcggtcaa
     1261 cgtaattaaa actataagcc tgatttaata cggcttcccg cagtgctagc ttcaaaattt
     1321 ccggctgata atggtcttct tctagccatt gtccgatccg ctggaactcg atcgaagaaa
     1381 gcgggcgtcc aaattcttgt tcaaataatt gatacactga ctggatttct ttttcttgtg
     1441 ttttttcttc gtattttttt tcttgtgttt ttaaatactc acctaataga tcatagatgg
     1501 ggtaaagatt atatcgatct gcttttttac cgttgacaat cgtcgtttct atcttgataa
     1561 atccatttgt tacgagacga tccagtattt gaaaaatctg atcttgtttg attcccatat
     1621 ccatagcaat catctgcaaa tcagggaaag tatctccttc aagctgtgcc atatgcaact
     1681 gtagaacaaa taaaaattct tcagattgta gaccgatcct atgataattt ttaagtaaaa
     1741 gattagatat gactgtttgt ccagcgctta agtaattttc taaattcaac atgatttctc
     1801 ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag caagaacagg tttcttttct
     1861 atttacttca ctgatcggtc agatttacaa tgtcgtctac ttgatttttg tttgttcatg
     1921 tttttgatgc gtatcttcac ttttcggaat aaataaatta agtagatttc gctgttcctt
     1981 agaatgttga caaggaaaca aaattactat acactatttt tgtttccaag ttaacattat
     2041 tgcataaagg agtttttaca tgtacagttt cacaaataaa cattctaaag tttttggata
     2101 cgtctctgtc ttattcactg gattaggatt tactatcgtc agtcctgtac ttccgttttt
     2161 aacgctgcct tattctcact ccatacatca gcaagctttc tatatcactt tgctcatgtc
     2221 tgtttatgct ctagctgcct tcttatctgc ccccattctc ggtagcttaa gtgatcattt
     2281 tgggcgtcat ccgatcctta ttatcagttt attaggatca agtatcggat atttgctttt
     2341 tggtttagga aattcgatat ggatgttgtt ccttggtcgt atcattgaag gattgacggc
     2401 aggagaaatc tccaccttat atgcttactt tgccgacatc acggaaccta atgaaagaac
     2461 caaagttttt ggatggatgg gtgctttagt cggtatagga acaacgctgg gtcctattat
     2521 agggggtcta ttagctgagt tggggaattc tgttcctata tttattggag cactctttac
     2581 tttcttaaat gccgtttatg gctacacatt tatgctagaa agccttccaa taaaaaaacg
     2641 ttcagtagat ttaagtttct cccatgtaag accttttcac caattacaac aattattcaa
     2701 gatttcatcc gttattcctt tattgacagc aggttttgct gtttggttgg cagctggctc
     2761 tcttcaatct atcttttctc aattttcgat tgacgccttt cagtggaagg ctgggttggt
     2821 tggtttaagc ttctctttga ttggaatctt ggatactgtc tcgcaattgt tcattatgcc
     2881 tcgattgctc aaaaaatttt cagaacagca gatcacaaga atagggatgt tcagtgaaat
     2941 cctcgcctat ctattcatca cattatctgg aattcttcta ctgccgattt tgtttttatt
     3001 tgggattatc tgttatggtt tcggtgattc tatttttact cctgtattca atggccaact
     3061 atccaattct gtctcagata accagcaagg tcttgtaatg ggcggtaccc aaagtataca
     3121 atctctatcg cgcgtcatcg gcccgctaat cgcaggacag ctttatgctg cagcaccatt
     3181 tcttccgacg gtattcggat ttttgcttat gatttttgcc tattatcact acaataaaaa
     3241 atagtagctt catttgctac ttcgtataaa ttctctgtat cgaccgataa aacgaaatac
     3301 tgtgtcaaat tcttcttttg acatattttc aaatacagta gcatctcgct gtacccattt
     3361 ttgatggagt tgctcgtgtc tattccttat tttttgtccg attggcgtca attgaaaata
     3421 gatttctttt ttattctctt cgctttgata agtgtttatg atccctctag ctatcagttt
     3481 ttttgttagc ttactgatgg ctcctctagt catgtaacta gcattcgcta attttgtcac
     3541 gttaggatca gggattttcc cgatatgttc aatcacttct agttcattta gagtaaatcc
     3601 tgcaaattct ttttccattt ctggtttatt acgatgaata atctcattag ctaaatccag
     3661 atattcttct attagcagtt gtcttttatc catttctaca ccttcttttt ctttcattat
     3721 attgtttttt tgtttcctat tcaacaaaga taaaaatatt ttgtaggaaa acccaatctg
     3781 aaaccaagaa aaaaaggggt tgacaaagct tgtcataacc cctttttctg attaaaagat
     3841 agtaaagaga cagtcatcgg taattgtgat gattttttgt catcacttaa ggatgagcgg
     3901 ccttttcaaa aactgtcttt aaagatcatc atcagtttct agcacatatg gctgaccttc
     3961 atatgtgtac tctgtattca atgtgccgtc attttcatct gagtagatgc gtctactgaa
     4021 aggaaaacga tgagaaacat tatctttggc gtcatttgct ggcatcgtgt aatattcctc
     4081 ttgcgtttca ttcaactgag agagtacttg atctgccaac tcatcaatat tgatcgttaa
     4141 tttcttcgaa gcacttttat aatcttctaa aaatgcatct tgttggacaa aatattcttt
     4201 attcatcttc tttctcacct ttcttttcaa aatcaaataa agctgcttgt tcttttatgt
     4261 aacagcttta tctttgctgc ttattgtgac aaaaaattcg aagaaagaca actaattgta
     4321 cttttcacaa aaaaagacag agggtcgtga caaaattgtg tcacaaccct ttttctttat
     4381 tgtgcagttt cgatttcgcc tagcgctgct tgtgcaagta ggttcaagta attccatggg
     4441 cggtcaaaat gtggttggaa gaagaaatct gaaagtgcga gatcttctac agtcatttta
     4501 ttttgaacag ctaaagacat ggtatttgct gattgtgtaa tgtcgtattt ggacatgaac
     4561 tgagcaccta cgatacgatt cgttcctttt tcatagacta gctccatcat tactttttca
     4621 gtagttggca tgaactctgg acgataatta tcttcaaata cagtcgcttg gacttctaga
     4681 tcgttcaaag ttgcgctttc tttggtcaca cctgtcgaac cgatcgtcca gccaaatagg
     4741 taaagtcctg aagttccttg agtaccacga tacgccattt tatgatctgt caagttattt
     4801 cccacaagca aaccttgacg aacggcattt gttgccaaag gaatatagtt ttgcgtgcca
     4861 cttggattgt agttgactac tgcagagtct ccagctgcaa aaatgtctgg attacttgtc
     4921 tgcatgtatt cattcacttt gattgccccg tttggcagtg tatctacttt tccttcgatc
     4981 agttttgtat ttggacggaa gcccacacat aaaatgacca tatctgtatc aaatgtatct
     5041 gttggtgtag ctacttgttt cacttttcct tcttcatctg tgataaattc agtaacattt
     5101 tcgcctagcg caagagtaac accgcggtct gtcaattctt tttctaagat atctgtgaat
     5161 ggtttatcta gatatttatt caagatgcga tctaatccat caatcaatgt cacttgtttt
     5221 ccagattcga caaaagcttc tactaattca ataccgatat atccaccacc aacaattact
     5281 acacgttttg cttctttcgc ttgtgcaata atttcatttg cttgattgta gtttttgcat
     5341 aggagtacat ttttcgcatc gattccacta atcggtggga taataggcca tgaaccagtt
     5401 gtcatcacta atttatcata agaaaccgtt tctgtttctc ctgttttcag atcttttgcc
     5461 gttactgttt tattttctgt atcgacgtct gttacatcgt gttccatttt aactgttgct
     5521 cctaatgaag ataattcttc aggatttgag tagaacaagc ctgctgggtc ctttactact
     5581 ccgccgacat aaagtgcgat accgcaagat aagaaagaaa tattgtcatt tctttcatat
     5641 actgtcactt ctgcttctgg atgatttttt aaaatacttt ttacagctgc agtacctgca
     5701 tgagtacagc caattactac tactttcatt tatggtccct ccgattaaca ttttgtcctt
     5761 cgacaaaact actataacag aaataaaacg gatacaacag tttaatgctt gtgaattcac
     5821 aaaggtttgt ttgtagtttc attccttttc aagcataaga acccaatatc tacaagattc
     5881 ttattttaat aatattcact tttatatcta agattgtgaa taaaagacaa aaaaataaaa
     5941 aagctagcgt tttttttaca cagctagctt tttttaggta taacacttgt agaaactgtt
     6001 tttacatttg cgcctttttt gctgactctt tacaatccgg acagagtcca tacaattcca
     6061 accgatgttc attaatctca aaatcagtca gtctgcttgc agccatttct atatcttcaa
     6121 gccctgggta atgaaaatca acaatcttac cgcagttttc acaaattaca tgataatgac
     6181 gctttgaact gaaatcaaat cggctagaag catcgccgta cttcatttct tgaacaaatc
     6241 caatcgaagt aaataagcga aggttattgt aaacagtcgc aacactcata tttggaaacc
     6301 gatgttcaag tgctttataa atctcatcag ccgttggatg ggaatgatgt tcaatcaaat
     6361 attctaaaac cgcgtaccgt tgaggtgtga ttcgaatgtt ctcttttttt aacttttcga
     6421 taacctcttc gactaactta ttttccatgt aaacacccca cgttgttatt ctcgtcttat
     6481 catactattt ttacatttta aaatccactt aacatgaaaa gaaaattcaa ttttattgta
     6541 ttttaatgaa aaaagacgta aaacagtcgc gttcactatt ctacgtctct attcaaataa
     6601 tttttctcat ca
//
LOCUS       DACDCJ010000116         6545 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDCJ010000116 DACDCJ010000000
VERSION     DACDCJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.117"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..395)
                     /locus_tag="IV038_002648"
     CDS             complement(<1..395)
                     /locus_tag="IV038_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP8342806.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFT"
     gene            845..1459
                     /locus_tag="IV038_002649"
     CDS             845..1459
                     /locus_tag="IV038_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8342807.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1624..1866)
                     /locus_tag="IV038_002650"
                     /pseudo
     CDS             complement(1624..1866)
                     /locus_tag="IV038_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2070..3389)
                     /locus_tag="IV038_002651"
     CDS             complement(2070..3389)
                     /locus_tag="IV038_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP8342808.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(3544..4131)
                     /locus_tag="IV038_002652"
                     /pseudo
     CDS             complement(3544..4131)
                     /locus_tag="IV038_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4769..5026)
                     /locus_tag="IV038_002653"
     CDS             complement(4769..5026)
                     /locus_tag="IV038_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP8342809.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(5019..5288)
                     /locus_tag="IV038_002654"
     CDS             complement(5019..5288)
                     /locus_tag="IV038_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP8342810.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(5419..5598)
                     /locus_tag="IV038_002655"
     CDS             complement(5419..5598)
                     /locus_tag="IV038_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342811.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(5979..>6545)
                     /locus_tag="IV038_002656"
     CDS             complement(5979..>6545)
                     /locus_tag="IV038_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342812.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         2041 a         1198 c         1025 g         2281 t
ORIGIN      
        1 gtaaaaagat tcagtgatcg ggtcactttt aagattgatt catcgatcga gtaaatcagt
       61 agatcttcat cagccacata tcttctgaaa atattattta cctgcatatt tcgcttgata
      121 tataggttca tacgtggtgg aacaacgtgt agtgttttag gaaatggttg tggtaagtca
      181 cgtggtctac tcacatttgt aataccatac cgcttttttg cttcaggaga agaagctaat
      241 atcaatcctg aaccagtatt atcagctcga ctcataacaa caagttctgt tgttaatgga
      301 tctaaatttc tttctataca ttcgacactt gcgtaaaagc ttttcatatc gattaaaaag
      361 taatcattta ctggctcttt tgaataatcc atcataaata acactccatt ctttcacatt
      421 atacaaacat atgttcgata ttgtaaagaa gaaatagcta aatacataaa aaaagaagca
      481 taggtatgct atcaccctgc ttcttctttt gatttgtatg atcaatttat tctatcactt
      541 tactaattaa tttgaaaatc taataaaaac tctcactcta aaagagtgag agactgcaaa
      601 ttagagtacg gacttttttg gagttccgtt ctttaatatt aacaaatgaa taaaatcctt
      661 acaacatttg agaataagtt atcagtagac acttacaaaa aattctcata ctatttcctc
      721 catcattagt ttttgtctat ataaggttta ccctaaatag acactggtat aacattgaaa
      781 aatatggctt cctcgtgtat gattagggtg tattttaaat agttgcagaa agtaggtaaa
      841 agttttgaga gttgcataca tacgtgtctc atcgatcgat caaaatgaac aacgccagat
      901 tgaagaaatg aaaaaattcg gtgcagagag aattttcatc gagaaacaat ctggcgcaac
      961 gattactcat cgacctgttt tccaagaagc attagattac gtccgagatc aagatatttt
     1021 tattgtggaa gctattgatc gacttggaag aaattatgac gagatcatcg cttcagtcaa
     1081 ttacttgaag aagaaaaatg tgaagctgat cattactagt ttaccgatca tggctgaagc
     1141 aattggcaat cctctcctgg ataaattcat taaggatctg atcatccaaa ttttggccat
     1201 gatcgctgaa caagaacgga ctgaatcgaa acgtcgccag gcacaaggaa ttaagatcgc
     1261 aaaagcaaac ggcgtctaca aagggcggcc caaattatac agtgcagacg caaaagatcc
     1321 ccaacgtcgc cttgtttaca aaagcatcgt tgaagatctt aaaaatggtg ttgcgattgc
     1381 taagattgct aaagactaca atgttacgag acaaaccgtg tatagaataa aaaaagacag
     1441 catggtcaat gacaaatagt tgatcatgct gcttttatta tcttctcatc tttaatttcc
     1501 tacatataat tactatctat aaaattaagt cttactcaga acagataaag cataaaattc
     1561 caacaaacca ttttttactt aactaaataa agagtctgtt aatataatct tcacttattt
     1621 acgttaatcc cctactgtat ttgttggttc cttatctata ttttctacaa aatccttatt
     1681 atcttgtgat ttagcttcgt ataattcttt gtatgtcgac acattggtta tactaattcc
     1741 tatagctagt tcttgagact ctacttttct ctctagctcc tttgacaaag tgatttgaga
     1801 tttatttggt tccgttggag tcaattctat agattcttca taaaaatctc tatacataaa
     1861 gctatccctg aataattcat agctttaatt ctttggcaca ttttattgaa atatgtatca
     1921 ctaatatccc tatcaactga ttttgactaa aaagttactt caaacattca ctgttcaaaa
     1981 atattgagga ataaattttg ggtgtaaggc attttgagca tacttctccc tggataactt
     2041 cccagtaaaa aaatcgttag attgttggat tagatccatt gactggctcg cctcaggcga
     2101 gcaaagaccc tgtagaattc attctgatac acatgataac tagataattt gagatagact
     2161 cgtctacccg tttggactaa tttcccagca actttcagaa acttcaatcg aatagaatga
     2221 atggtcattc ccttttggac ttcttcaaag ccaatcgttc ttaagaagtt gactaagttg
     2281 taagctatca agctgagcat cattcggaca tgattctcca aaaagcgagg actgtctgtt
     2341 ttgtcaaagt aaaagccagc tttcgcttct ttaatgaagt tctccattgt gccacgtttg
     2401 gcatagatag aaaagatggt atcaggagaa acattttctg atagattcgt cacgataaat
     2461 tcatgtcgaa agagtagttc gcccgcttca cgtgttgagc gtatacacac gcgacgactt
     2521 tgtgaccagg attgtgcttg ataagtggtc gagaagtact gaacttctcg ttcttcccac
     2581 ttttgattat cgccataaag tactgatttc tcagctattt gacctagtct acgattattc
     2641 ttcagtcgaa taacataatg actctttttt gattcacacg aatcatacac accaggtgta
     2701 gcgaacccgc tgtctccacg aaccaagatg tcagtgtttg gtagagagtg attatagtgc
     2761 tctaataaag gtgtgagaaa ctcctttacg ccttttgatg tatattgatt tcctgaacgt
     2821 agttcagctt ttaagaagtc accagtcaat ccgtcaaaag ctaccaatgg atggtaacca
     2881 taggtttggt agtgtgtatt ataattcgtt tgttcttggt gaccaaatgt atctgaatgg
     2941 gtcgaatcta aatcaatgat taattccgta tcattacgga tgaggcgtgc tttatcgata
     3001 agttcttggt tcaaagcttg aagttcatgg atattctcct cagatagtcg atctaaaaat
     3061 cgggaaagtg aagattgaga agcgagttct ttcctatcta aaacagcttt aaacacagga
     3121 tcttgtctca ggagattagc tgcagaatca gctgagtaac cagcaattaa ttgcataatg
     3181 aactgctcaa gtatggataa gttgtcatga gtaaaatacg ctcgttcgtc ttcaatgtga
     3241 atgtgttgct tagccagttc agaaaaacca aagatgttca tcagctcttt cactaggacg
     3301 agacccgaat cactcgataa ttgaccacct gtatgagaaa tagtgatatt tgaattgaat
     3361 tttacttggt ttttgtgtaa gctagtcatg aaaagaactc ctttcttttg gttggtttag
     3421 tcactttaac catagcagaa aggggttctt ttttcatcac ttaacgggtg aagatgaaaa
     3481 agtaattgta agctgccgtg aggcagtttc acatggtttt aattaaaagt atgaattatt
     3541 caggctatca ttaattttat aaaaaaaatt aaatgttttt agcactgcca tataacattc
     3601 aaaacattct tctatagttg gtttacttcc ttgaggagaa tgaaccaatt tattacgttt
     3661 ttctatgaaa tttttaattt cctttcttat ttctttcgga aactcaatat taaagtaccc
     3721 tttaaacatg ctccctaata atttgtctac agggggagat tgtaaattct caaatattag
     3781 agttaaatca ggtttctctt gttgataaaa ttcctttacc cctatttcac aggctgtaat
     3841 tatcgaaata aacctatctc tagtatttga tatattttta cttcttttaa ggagtataag
     3901 agaaaagggg atactttcgt gtccatgcat gtaattaact aattgttgag cttcagaagt
     3961 tatatctcta attatatagt taacggaatt aggaagtgtc tttaactcta atgaaatttc
     4021 tatccaatta ctatcttcta atctgtatat gctcgaagta taatttgatt cgtcagcaag
     4081 catcgctaca tcccaaacac tgatcataaa atcttttaaa cgttcaatca tttcagcaat
     4141 attgctaaaa tcagctaatt tttctttaga ataaaaaaaa acatttatat cagaatcaat
     4201 aaattctatt tttttattac tatatcgtca tcattatctt gaccatttat gtttattgtt
     4261 ccctgagtgg ggagtacttc tttcaaattc aagttaaatt tatattccat tacttctcct
     4321 ccaatatata aaaattactt aactgtatcc aaatttaata tgtaaataaa tcattaattt
     4381 ttagctaaat tctatgagaa gtttattttt gttgtttttg ctaattaact aaaactagta
     4441 caaaaaattt aaaatagatt attaaaccta ttgcttattc tattcagtca tatatataag
     4501 cgtcatcctt tagtaactaa catcccacac tacaatagta ttataacata tctattcaac
     4561 ttcaaattgt tgaaatttaa gccatttata tttacttttc ctcaacttta gaaatgattt
     4621 tactaacctt atatccgtaa tacgcgtaac aaacacattc attaataaca atcaacacta
     4681 agctatatta gctttttaaa tgactagggt aaataaaata aggatcaaaa acttttaagt
     4741 ttctgaccct ttccttactt ccgattggtt aatagtgatc ttttgcagaa taaataaata
     4801 tcgtttcatt ttcaactcta tatatcagtc tatgttcatc tgtaattctt ctggaccatt
     4861 ttccagataa atcatgcttt aatggctcag gttttcctaa tccagcaaag ggggaacgat
     4921 cgatatcttt tattaacttg ttaatctttt ttatattgct tttgtttcct tgctcatgcc
     4981 aataaagata atcatcccaa gcatcatcag accaagcctt aatcatcaga ttcaacctcg
     5041 attaagtcat gtgttttaaa tgcacctttg gagaattgtt catctcctcg acgaattttt
     5101 tccatgacgt aattattaga aagtgttctc aacgtttctt gcatagaatc ataatctctt
     5161 tttgataata caacaactgt atcttctaca tctttacttg ttacaataag tgtttcagca
     5221 tcctcattaa cttgtttcat ataactacgt aaattttggc ggaaatttga ataagctact
     5281 gcttccattc ctttcacccc ttatttattt ctataaaaca attgtacatt atattgtaca
     5341 attaagcaaa tgattaatat tctaacttct tataattaaa agtgctatca cttttagttt
     5401 ttaggataaa aagtgacgtc actttttgag tgctggtacc tcaaatcctg ctttttcttg
     5461 ggcaatcaat aaatctaacg caatacacat aatcctggat tcacttttgc cgtacttctg
     5521 ctttagttgc tgcaattctt tttgtctctt ttcatccaac gtaacgggta accgtttatt
     5581 tcctattgca attggcatta gtactctctc ctttcatcga aataacccta tgaaaaatat
     5641 ggctgcaatc atcttgaata gtagactcac aagggctaat acaaagcgcc aaaagatcaa
     5701 acaaaatacc agtattgcca aaaatactac gaagtttaaa tgcacagctg cagcatggcg
     5761 cataagtatt tcgccaacaa ataggactaa caatagagga atgattttat tcatatggat
     5821 ctccttcttt ctcttaggtt atcgggtgtt gcccgcttat ttcgcaccac ctaagcggcg
     5881 ggggcggttg gagcgattgg tgcgaaataa gaaccattcc cttagttact aggttttttg
     5941 tccttatttc acacattctc acttttcggt ataaatgatt attgcctttt tttcctttca
     6001 aatcgttgat atggcattcg tttacggtca agttcatttt ggatttcttc tgtatcgtcg
     6061 taagtatagg tgtctaaaaa atcagttaaa gtcgtgtacc agtcgtcgcc tgggttatct
     6121 acttcttcta catattccct caaaaatgct tcgggtacag cgagttcata atccattccc
     6181 attaacacga tagattccgt tccacacgtc aaaaatctag taaatacaag gtaataatcg
     6241 tctatcgtcg catctttgct gtttagctct tccatttctt cttccattaa ttccatgctc
     6301 tcaaaaattg aataatattc tgattctgag taatagtgtt tcctgcaaat cgtgtagctg
     6361 acaaagtttt cgaccagctc actcctttct aggtaaacat tcatgatgta tttttctgtt
     6421 tctagtcgta gaagctccgt tccttttttt accgtttcag gacgacgttt atacacgtct
     6481 aaacgattat ggctgactgt gaccttcgtt tgactcgttt cactttccaa atacaattgt
     6541 tcttc
//
LOCUS       DACDCJ010000117         6500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDCJ010000117 DACDCJ010000000
VERSION     DACDCJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.118"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IV038_002657"
     CDS             complement(<1..142)
                     /locus_tag="IV038_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8342813.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IV038_002658"
     CDS             complement(183..437)
                     /locus_tag="IV038_002658"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8342814.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IV038_002659"
     CDS             complement(548..802)
                     /locus_tag="IV038_002659"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8342815.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IV038_002660"
     CDS             complement(995..1270)
                     /locus_tag="IV038_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP8342816.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IV038_002661"
     CDS             complement(1242..2195)
                     /locus_tag="IV038_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8342817.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IV038_002662"
     CDS             2807..4300
                     /locus_tag="IV038_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP8342818.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IV038_002663"
     CDS             4435..4731
                     /locus_tag="IV038_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8342819.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IV038_002664"
     CDS             complement(4755..5714)
                     /locus_tag="IV038_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8342820.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..>6500
                     /locus_tag="IV038_002665"
     CDS             5897..>6500
                     /locus_tag="IV038_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342821.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT         2082 a         1092 c         1144 g         2182 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagcaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga
//
LOCUS       DACDCJ010000118         6427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDCJ010000118 DACDCJ010000000
VERSION     DACDCJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.119"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(18..920)
                     /locus_tag="IV038_002666"
     CDS             complement(18..920)
                     /locus_tag="IV038_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP8342822.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            1106..1675
                     /locus_tag="IV038_002667"
     CDS             1106..1675
                     /locus_tag="IV038_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP8342823.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1733..1912
                     /gene="rpmF"
                     /locus_tag="IV038_002668"
     CDS             1733..1912
                     /gene="rpmF"
                     /locus_tag="IV038_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP8342824.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            2082..3503
                     /gene="gndA"
                     /locus_tag="IV038_002669"
     CDS             2082..3503
                     /gene="gndA"
                     /locus_tag="IV038_002669"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP8342825.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            3696..4382
                     /locus_tag="IV038_002670"
     CDS             3696..4382
                     /locus_tag="IV038_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8342826.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            4379..5884
                     /locus_tag="IV038_002671"
     CDS             4379..5884
                     /locus_tag="IV038_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8342827.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         2187 a         1061 c         1345 g         1834 t
ORIGIN      
        1 taaaaacagc tcttctttta cgaagagctg tttttttgtg ctgtaacgaa ttcatggata
       61 acttggacta cttgttcttg aagaggcgtg acgatctgct gtgttcgttt tgccaatgaa
      121 atcaagaagc gaggctgaac ttcatctgaa agagggaggc aaactaaatg atcttcagga
      181 tggacagcaa tctctgccaa aaaaccgata cccacatttt ctcggaccat actctttaaa
      241 atcgtcaagt cattgctttg ataaacaact tttggatgag tatgtgtttg tttagaaagc
      301 ttagtgaatg ctgcggggtg gacataatgt tcgttcaata gtacaaactg ttcatctgct
      361 atatctttga aagagacagt atcctgcttg gaaagaggat gtgtaggtgg cacgacgatc
      421 atgaaacgtt tgtccaaaag taactctgtt tctaaatatt gatccacgat tggctgtgtt
      481 gagcctaata cagctaaatc gatcctccct tgtcggatca tcccgtataa gtcccgagag
      541 cctccatcgg taatctcaat ttgattcatc aattcatgct tgataaagta acttgacagt
      601 ttaggaaagt attcattgcc gataatagga ggcatgccaa aacgaagctt tttcttttgc
      661 aactgagtga tttcagctct tgcagtagaa atctcttgga gaatggtttg tgcatggaga
      721 aacagctgct ttccagaagg cgtgacgatt aattcgtgat gcgatcgatc tcttgtaata
      781 agtacggtgt caaactcttt ttcaagacgt tgtaatgcat atgtaatagt aggttggctc
      841 acacggaaat aattagccac tttcgtaaaa tttttttgtt tgacgatttg gacatagtat
      901 tcgatatctt tcgtgttcat gaagatcatc cgctccttta atggaataat gattattgta
      961 aatgattctg aggatgatag caaacagagg ctttctaata aagatttttc tgttttttta
     1021 tgaactgcaa ggtgtcaaga aaatttcatt gacaacatgc tttttgccct gtataattgc
     1081 ttttgttgct taggagtgat gaaaaatgaa atggtcttta ttggaattga gaaaatacca
     1141 ggagacaccg ttaacttttc atgagacgct cgacttgaaa gccgctctca tgaagcgaga
     1201 caatttaata cttgatgttg cgccagtgga agtcgaaggt ttggtctcag ttgataagaa
     1261 aggctatatc gttcattaca ctgtgcagac gactttgaca gttccttcaa cacgttcact
     1321 agaacctgtt gaactgccca tggatttttc tgtagacgaa gtattcatga cagaagaaca
     1381 attccaaagt agagatgaac aaatgtctgc ggatgagatt ttgctaattg aaggtccatt
     1441 attgaattta gcagagtcga ttgaagacaa catcttattg gcgattccaa tgcgggtatt
     1501 gacaaaagaa gaggaagcag caacagaact tccaaaaggc aatgattggg aagttgtttc
     1561 cgaagaagaa taccagcgca gaaaaatgga agaagccgag aataaaattg atcctcgtct
     1621 tgcaaaacta tctgaacttt ttgattctaa atcaaaagac gacgataaca agtaagattg
     1681 gaatatattg tgtctaattt agatacaaga ctttacaagg aggtgtagca aaatggcagt
     1741 accagctaga agaacatcaa aagctaaaaa agcaaaacgt cgtacacact acaaattgac
     1801 aatcaaaggg ttaaacgaat gtccaaactg tggtgaaatg aaaaaaagcc atcacgtatg
     1861 tgcaaattgt ggttactatg atggtaagga cgtaatgtca aaagaagctt aatacatttt
     1921 aattagaaaa tgtaaaacct caaggctaac catggagcct tgaggtttat ttttttgtag
     1981 tcggtaaata aaaaatatgt tgttctttag tttgcaatgt agccgtgtta agctataatg
     2041 taagagatta gggagaaatc ccgtatgtag gaggagttca tatgtcaaaa caacaaatcg
     2101 gcgttgtcgg tatggccgtt atgggtaaaa atctagcctt gaacatagaa agccgcggct
     2161 attcagtggc tttattcaat cgtacagggg ctaaaacaac cgctgtagta gaagaacacc
     2221 cagataaaaa tttcaaagca acatatacaa tcgaagaatt tgtagaatct atcgagaaac
     2281 cacgtcggat cttgctaatg gtcaaagcag gaccggcaac agatgcaacg atccaagagt
     2341 tgcttcctca ccttgataaa ggcgatattt tgattgacgg aggaaataca ttctttaaag
     2401 atacgatgcg tcgtaatgaa gagttagcca actctggcat caactttatc ggtacaggcg
     2461 tttccggtgg tgaagaagga gctttaaaag gaccttcaat catgcctggt ggacaaaaag
     2521 aagcctatgc gctagttgca ccaatcttag agcaaatttc tgcaaaagca gaagacgaag
     2581 caccatgtgt cacatatatt ggcccaaatg gagccggaca ttatgtgaaa atggtacaca
     2641 acggtattga gtatggagat atgcaactga ttgctgaatc ttatgactta atgaagaata
     2701 ttttaaacct ttcagtagaa gaaatggctg atatctttaa agaatggaat caaggcgaat
     2761 tagacagcta tttgatagaa atcactgctg acattctgac acgtaaagac gatgaaggaa
     2821 caggtaaacc agtagtagat gtgatccttg atgcagctgg gaataaagga acaggtaaat
     2881 ggacaagcca aagcgcgctt gacttaggtg tgccacttcc attgatcact gaatctgtat
     2941 ttgcccgcta tatttctgct tataaagaag aacgcgtaca agcaagcaag attttatctc
     3001 gtacgaacga ttttgagttc acgggagaca aaaaagaatt ggtcgaaaaa atccgagaag
     3061 cgttgtattt cagtaaaatc atgagttatg cccaaggatt tgctcaatta cgtgtcgctt
     3121 caaaagaatt tgattgggac ttgccatttg gtgaaatcgc aaaaatctgg cgcgctggat
     3181 gtatcatccg agctcgattc ttgcaaaaaa ttacggatgc ctatgacaaa aatcctgaga
     3241 tcgaaaattt attgctagat gattacttcg ttgaaatcac gaaaaaatat cagcaatcag
     3301 tcagagacgt cgttgccttg gctgtccaag ctggcgtacc tgtaccgact ttctcatcag
     3361 ccatcgctta cttcgattca tacagagcag aacgtctgcc tgcaaatata atccaagccc
     3421 aacgcgatta ctttggcgca catacctatg aacgtgtcga caaagaagga atcttccatt
     3481 actcatggta ccatgaagaa taatagtctt tcggctgtta ggaaatgaga aaataaaaag
     3541 aggacgaaag agctgaagtc gattgttatg agtagacttc agctcttttt taaaattgct
     3601 atccatgata aaatgactat agatttttaa gcatattatc ggtataatga tagagttttt
     3661 aaaaagccag tagaatagaa aggataaaat aataaatgag caatattcta atcattgaag
     3721 atgaaaagaa cttggcacga tttgtcgagc tagagttgaa gcatgaaggg tataacgcag
     3781 aagtccacta caacggccga actggtttag atgcggcatt aaataatgag tgggatgcca
     3841 tattgttgga tctgatgctc ccagaattga acggtttaga agtctgcaga cgtgtacgcc
     3901 aagtcaaaaa tacaccaatc attatgatga ctgcaagaga ttctgtgatc gatcgtgtat
     3961 ctggacttga ccacggagca gatgactaca tcgtcaaacc atttgctatc gaagaattat
     4021 tagcacgtct tcgtgcattg ttgcgtcgaa tcgatattga aggcgataaa aatgtggcta
     4081 aacaaactac tattacttat cgcgatttga ctatcgaaaa agaaaaccgt gtcgtacgtc
     4141 gcggaaatga agtaattgaa ctaacaaaaa gagaatacga gttgctgctt actttaatgg
     4201 aaaacgtgaa cgtggtatta gcacgtgacg ttttactaaa taaagtgtgg ggatacgaaa
     4261 cagaagttga aacaaacgta gtggacgtat atattcgtta tcttcgcaac aaaattgacg
     4321 ttcccgggga ggaaagctat atccaaactg tacgcggaac aggttatgtg atgcgctcgt
     4381 gataaagccg aaaaaatcgg aaaaaagaga actgaaaggg ccctccttga caatcaagtg
     4441 ggcctttgcg agttctttct tcatattcgt tgtgtttaca attttcgctg tcattaccta
     4501 taaatcatct atcaatctga ttgtggcaaa agaaagaaac aatgtagagc gtacgatttc
     4561 agaagtagct tctcgtttgg ctaactcgga tgaagaactg acattagtaa atacgtatcg
     4621 taatctgacg aaagcgacaa atgacggaga tgaggtcgta gatcatactt cactggtcga
     4681 aggaaactta atgaagatcg attcctttat agctgagcta gggcagccat caatggatct
     4741 ttatgtttat gatttggacg agaacttgat ttttaaaacc catgaaaaca gtcgaaacct
     4801 gattcagact acacgtaaag ttccaacgat cgttacacta gaaggaaaaa ctggcttttt
     4861 atctatccag cctatatatt ctaaaggtac aagagaaaag atcggctatg cgcaaagctt
     4921 ctatgaatta tcttctttct atgatattcg aaataagcta ttattgactc ttattgtttt
     4981 agaagttgtt tctttgatct taagtagtat tttaggtttc tttttatctt cttatttctt
     5041 aaaaccactc aaggtactca gagatacaat ggatactata cgtaaagatc cgcaatcaga
     5101 tatccacatg cctgaaatcg acacaaatga tgaattggca gatttagctg aaatattcaa
     5161 tgagatgctt gaccgtatgc gtttgtatat tgaacaacaa gagcaattcg tggaagacgt
     5221 atctcatgag ttaaggacac ctgttgcaat tattgaaggg catcttagtc tgctaaatcg
     5281 ttgggggaaa gatgatcctg agatattaga agaatcatta agcgccagca tgcaagaaat
     5341 tagccgtatg aaaagcttag ttcaagagat gctagactta tcaagagcag aacaagtaga
     5401 tgttcattat gcgaatgaaa caactaaagg aaaagaagtc acctatcaag tttataataa
     5461 tttcaagata ttatatccag actatgtgat cactttagat gatgatttga caaaagaagt
     5521 gaccctgcaa atttatcgta atcactttga acaattaatc attatcatat tggataacgc
     5581 agtaaagtat tcaactacac gaaaagaagt ccatatctct atttcttcca ctctgaatga
     5641 atttgagatc gcgattcagg actttggcga aggaataccc aaagaagacc ttaataagat
     5701 cttcaatcgt ttttatcgag tggataaagc aagagctaga acgaagggtg gaaatggtct
     5761 ggggttgtct atcgctaaac aactggttga aaactataaa ggacgtatct tagcagaaag
     5821 tgtagtaggg caagggacaa tttttagaat ctttattcct atcgcaaagg cagaagacaa
     5881 gtaaagcatg tgctttactt gtttttttat atatccaaca aagaataagg tacaaaatac
     5941 aagcaagtta gaaattcagg gatgtcatta taaaaaaaac tagcgaacaa ttaaatagaa
     6001 taacatccgt taattaaacg taagtagctt ttagatgaac gaaaaaacaa tgaaatgctg
     6061 taatattcgt gtttaaaaat aataaataaa aatagggaat tttctattgc aattttataa
     6121 agagattgct atattattat ttgttctaaa tgattccttt aaggtgtcac taaaaattat
     6181 aaaaaatagg gttgacgcac ttttgatgtc atgttataat aacgaagtcg tttggatgag
     6241 gtagctaata aagcttgatt ttatcgaaaa aaacttcgaa aaagttgttg acaaataaca
     6301 ataaagatga tatgataatc aagttgctgc taatgagtga caaagacgtt tagaaaaaat
     6361 aaacaaagaa gttgttgaca ttctcttctg aatctgataa gattaaagag ttgttgaaac
     6421 aaaaaga
//
LOCUS       DACDCJ010000119         6367 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDCJ010000119 DACDCJ010000000
VERSION     DACDCJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.120"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            71..1666
                     /locus_tag="IV038_002672"
     CDS             71..1666
                     /locus_tag="IV038_002672"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP8342828.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            1656..2873
                     /locus_tag="IV038_002673"
     CDS             1656..2873
                     /locus_tag="IV038_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP8342829.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2886..6035
                     /locus_tag="IV038_002674"
     CDS             2886..6035
                     /locus_tag="IV038_002674"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP8342830.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2230 a         1050 c         1320 g         1767 t
ORIGIN      
        1 ttataaaagg gggtatgcta aaataagagg gtatatagca acttaaaata agagatagga
       61 gtgtatcttt ttgggtgcag aattaaatca aaaattattt agtgcggcag ataatctccg
      121 tagtaaaatg gatgcgtcag aatataaaaa ttatttattg ggactgattt tctataaata
      181 tttatccgat cgattgttgg aacaagtggt tcttttagca gatgaatcct tagaggaata
      241 tgacacagta agcaaacaaa caatgttgta tcgtgaatta ctttcagatg aagaatcaaa
      301 agaagatctg attgccacaa tcgttgatat tttgggatat gcaattgcac cagagtatct
      361 tttcaacgtc ttagcggatc aagcaaaaca agcaacgttc caattgaacg atctaaacaa
      421 agcctttgtg caattggcat ctacctacaa tcaatttaat gggttatttg atgatgtgga
      481 tctccaatca aaaaaactag gaacagatga gcaacaacga aatgtgacga ttaccgaagt
      541 catcaagaaa ttgaatgatg tagatgtttt agaacacgat ggggacgtga tcggggatgc
      601 gtatgagttt ctgatcagtc aatttgcttc agaagcaggg aagaaagccg gagaatttta
      661 cacacctcat atggtttctg atatgatggc gcaaatcgta acgctcgatc aaaaggaacg
      721 acgattcttt agtgtttttg atccaacgat gggttcaggt tcattgatgt taaacgtacg
      781 taactactta acacacccag ataacgttaa atatcatggg caagaactaa atacaacgac
      841 atacaatcta gcaaaaatga acttgatttt acatggtgtc gacgccgaag aaatgaatct
      901 ccgcaatggg gatacgttga ataaggactg gccaacagat gaaccttaca catttgatgc
      961 agtagtaatg aacccacctt attcggctaa ttggtcagca gatacgactt tcttagatga
     1021 ttctcggttt aatcgttatg ggaaattagc gccaaaatcc aaagcagatt ttgcgtttct
     1081 tcttcatggg ttctatcatt tgaaggaaac aggtaccatg gccatcgtct taccacacgg
     1141 agttttattc cgtggcgctg cagaaggggt catccgtcaa aaattactag aagatggcag
     1201 tatttatgct gtgatcggta tgcctgcgaa cttgttcttt gggacatcga ttccaacgac
     1261 ggtgattgtg ttgaagaaga atcgtcaaac tcgagatgta ttattcattg atgctagtcg
     1321 tgaatttgtt aaagggaaaa atcaaaataa actatcggaa gaaaacattc aaaaaatcct
     1381 cgaaacctac gctgaaagaa aagatgtcga gaaatacgct catttggcaa cctttgatga
     1441 aatcaaagaa aatgactata acttaaatat cccacgctat gtagatactt tcgaagaaga
     1501 agaaccaatt gatatggttc atgtcggaaa tgacatcaag aagatacgac aagaacagca
     1561 agtgctagaa aaaaaacttt tagaagctat ctcttccctg caaacaacac ctgaaaatga
     1621 agcgtggtta caaggtgcgc tggaggtgtt caaacatgag caatgataca caaccagaaa
     1681 tccgtttccc aggatttaca gatgattggg aacagaggaa gttgggggac ttcgttgtag
     1741 attatgttga aaagacaagt gtacaaaatc aatttcctat gctaacttca tctcaacaaa
     1801 aagggatagt tttgcaagaa gactattttg caaatcggca agtaactaca gaaaataata
     1861 ttggttactt tgtactacct agaggatatt tcacgttccg tagtcgaagt gataatgatg
     1921 tgtttgtgtt taaccgtaat gacattattg atagagggat tataagctat ttttatcctg
     1981 tatttacttt aaaatctgct gattccgact tcttcttaag acgtattaac aatggaatcc
     2041 aacgacaatt atctattcaa gcagagggaa caggtcaaca tgttctgtct ctaaaaaagt
     2101 ttaaaaatat agtagcaatg tttcctagtg aggaagaaca acaaaaaatc ggcaccttct
     2161 tcaaacaact tgacgacacg atcgctcttc atcagcgtaa gttagatttg ctgaaggaaa
     2221 cgaaaaaagg attcttacaa aaaatgttcc ctgaaaacgg agcaaaagtg ccagaaatcc
     2281 ggtttcctgg ttttacggaa gattgggaag agcgtaagtt gggagaatta ggtaaaactc
     2341 aatcgggaat tggatttcct gatgccgagc aaggtggttc tgaaggtact ccatttttta
     2401 aagtttccga catgaataac attggtaatg agtatgaaat gagaaatgca aatcattatg
     2461 tatctaatga gcaaattgag cgtaaaaaat ggaagccaat aaaagatgtg ccagcgatta
     2521 tatttgctaa agttggcgca gcaataatgc ttaatagaaa acgtttggtc acaagccctt
     2581 tcttaataga taacaatacc atggcttatt tatttaataa tacttgggat atctattttg
     2641 gtaaaatatt gtttgaaact ataaatttgc caagatactc ccaagtaggg gcattgcctt
     2701 cttataacag ttctgatatt gagaatatca gtgttaaggt tcctgtaaag gatgaacaac
     2761 aaaaaatcgg tactttcttc aaacagctag atgatacgat cgctcttcat caacgtaagt
     2821 tagatttact aaaagaaacg aaaaaaagct tcttacaaaa gatgtttgtc taggagggaa
     2881 gaagaatgaa aattagtcac cgtgaggaag cagaagtcga agaacagttg attcgtgtac
     2941 ttggagaagg gcataatcag tggacgtatc gtccggatct aaaatcagaa gaagaccttt
     3001 gggtcaattt acgccaaaaa attatctcaa ataatcaagc agaactgaat gattttcctt
     3061 tgaccgataa agaatttgaa acgatcaaaa cggaattact gctgcgaacg aaaaccccat
     3121 ttgatgctgc gaaatggtta aaaggtgaaa atgggatggc tcggatcacg attgaacggg
     3181 aagattcgca attgggttct gtctcactta tcctttattc caatcaagac attggtggag
     3241 gaatctctac ttatgaagtg gtccatcaaa ttgcaaaacg tggaagtaac atagaagcac
     3301 gggatcgacg ttttgacgtc accttattaa tcaatggatt accgattgtc caaatcgagc
     3361 tgaaacaagt aactgcaaaa gatggtgttt atcaggcatt taaccaaatc aaaaaatatg
     3421 cagaagaagg tatgtttcgg aacaatatct tttcgacctt acagttgttt gtggtttcca
     3481 atgaacaaac cactcggtat tttgcaaatg ccttaccgaa agacttgcac cctaagtttt
     3541 tatttagttg gcgaacgaaa gacaatgaaa aagtagaaaa cttatatgag ttttgtaaac
     3601 aagtgttaaa tatcccggat gctcatcgtt tgattgcgga ttatacaatc gtcagtgaag
     3661 atcaagacaa taaaaccctc atggtgttac atccttatca agtacatgcg attcaagcct
     3721 tgtttattgc cgcaaataaa catcaatcag ggtatgtgtg gcatgcgact ggttcaggaa
     3781 agacgttgac tagttttgta tctactaagc ttttagcacg aaaatcgggg atcgatcgaa
     3841 cgatcatgtt agtggaccgc aaagatttag ataatcaaac caccacagag tttacaaaat
     3901 ttgcttcgga atttaatact gggatttctt ccgggaacgc caaagcgaat agcttgatcg
     3961 ttggaacggg aagtgccaaa gaattaagtg aaaccctgtt ggcagatgcc aatgcaaatg
     4021 ttgtaatcat tacaacccgt caaaaactag atgcggcgtt gaaatatgca aagaaacaag
     4081 aagagaaaaa aggaaccaat cgcttccaaa aattaatggg ccaacatatc gtgtttgtag
     4141 tagatgagtg tcatcgagcg ttaagtgctg aaaatatgga agaaatcaaa aaaatgtttc
     4201 cgaaatccac ttggtttggc tttacaggta caccgatttt tcctgagaat cggaaacaag
     4261 ccaaagggca attggcacgt acaacccatg atcagtatgg agaggtcttg catacctata
     4321 ccattaagaa tgcgctagaa gatggctctg tcttaggatt tcaagtagag catgaaaaca
     4381 ccgtggagcc gacttcacta gagaataaaa tctatcggaa attaaaagaa gtagaaacat
     4441 atgcagagta ttcatcagaa cagatcaatc gtatgattga tcaaatggaa cctgtcaaaa
     4501 aagaaagtta tttggaccca gctgtttttg aagcagatga acatatccaa aaagtcattc
     4561 ataaaatgtt ccgtccagat aatgcctata caaaatttga ttttcgaaat ggcaggccaa
     4621 cgaaatctgc aatcttaacc acgagttcca ttgacatggc caaaaagtat tatcgggcca
     4681 tcaaagagat gacgaaagag cctgattggt taacaaaaga gtttcccaat cagctcattc
     4741 gtgaaggacg aacgatggag gatcctgatt ttccacgaat cgctattacc tattcattag
     4801 aagaaaatag ccgagattca agtgcccaac aagaagaaat gcaaaaaatt attgaggaat
     4861 ataatcagta ttatgatact tcctggtcat tagcagacat tgagcgttat aacggagaca
     4921 tcaataatcg tttggcacga aaaagagcgg aattcaaaca atttgggaaa caaatcgatt
     4981 tagcgattgt agtggatcgt ctattaacag gttttgacgc ccctacgatt caaaccctat
     5041 ttgtggatcg taatctagag tatgcaggat tgatccaagc tttttcacgt acgaatcgta
     5101 cgtatcctga taagacaaaa ggattgattg tgacgtttcg taagccagcg acaatggaga
     5161 agaatgtcga ggatgcgaca aaactgtatt caaaagcaaa agaagaatca ggtttgattt
     5221 atccaagtta tcaggaatcc aaaaaaagat tcaaacaagc atatggtaag ttaaaagagg
     5281 tttcatccat ggaatccatt gatgaacata cgccacttga aatccgtatc gagtacgtca
     5341 aagcatttca agaattgaat cgtgcttatg aatcgttagt gacttatgac gattacaatg
     5401 atgacatggt gaaatctcca actttgaaca atcaaattca acttttggaa gaacaaattg
     5461 gcgtgtacga gaccgtgaag ggttcattaa tagaggaaga accagaaaaa gaaggagaag
     5521 atttttcaac gatcgaattt tatagtgaaa ataccactaa gttgtatgat attgattcaa
     5581 cttatattga tcaattacta ggaacttatg ctgcgaatag ttcagatgca cgagaagaaa
     5641 ttgaaaaagc tttggctaaa ttgaataaaa cagaaggagt caaagaagtt taccgacaaa
     5701 ttttaaatgc atttgatgtg gggactttag atagtaatga agatatcttt gtcattaaac
     5761 gtcgctattt tacacaagcg agtgatgatt tgattcatgt cttttccaat gagtggtttg
     5821 tttctgagaa agaattacat gcttctaatc tgcaatatct accaggtgaa gatccaattc
     5881 ctaatatgaa agcaattatc aacagtaagg attacgaagg atataaagcc aaacatccag
     5941 aagcgaagcc atttaaatac ccacaagaaa tgaagcgagc atggcgtaaa atgttagatg
     6001 atgagttgat tccacttgag aatgaattga ggtaaacgag aagagttaag aaaattgtac
     6061 gattcaaaga tggtagtcaa gtggaagtgt agaaaatagg gatagatgtc ttttatttgt
     6121 tcacatttta taacgaagta tttaagacag ctattgaata atgtttcctc cacctttttc
     6181 attcatttac cccaccccgt tgttatatga ccgtgttttg agaggaacca ataaaaagca
     6241 taggctaact aggggttcct ttttcttttg tttttttgga atccgcttaa gtgaaatgta
     6301 tctgaaatga aacggagata aagtgatttt ttgctataat gacaaggttt tagctgtttc
     6361 agatacc
//
LOCUS       DACDCJ010000120         6348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDCJ010000120 DACDCJ010000000
VERSION     DACDCJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.121"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1464
                     /locus_tag="IV038_002675"
     CDS             <1..1464
                     /locus_tag="IV038_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP8342831.1"
                     /translation="RRDSVVVALTPIPKQTQLTIDQQTITTLEDIPQGHKIALGDLKA
                     GDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVHPSHYLFENRTFQ
                     GYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFDHITILKHPYGCS
                     QLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGEYDEERVKFLVAQ
                     DVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLSGITANPLLGAFS
                     DYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFKQYFLSYGEPVYE
                     NPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLSLLQAPGNDLVAA
                     SALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDFNAGVLLEQPMEV
                     ILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            1503..2897
                     /gene="uxaC"
                     /locus_tag="IV038_002676"
     CDS             1503..2897
                     /gene="uxaC"
                     /locus_tag="IV038_002676"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8342832.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            3056..3739
                     /locus_tag="IV038_002677"
     CDS             3056..3739
                     /locus_tag="IV038_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP8342833.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            3741..4577
                     /locus_tag="IV038_002678"
     CDS             3741..4577
                     /locus_tag="IV038_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP8342834.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            4588..6342
                     /locus_tag="IV038_002679"
     CDS             4588..6342
                     /locus_tag="IV038_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8342835.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         2025 a         1227 c         1273 g         1823 t
ORIGIN      
        1 cgacgagaca gtgtggtcgt cgcattaacc cctattccaa aacaaacaca actgacgatt
       61 gatcagcaaa cgatcaccac tcttgaagat atcccgcaag gtcataagat tgcattgggt
      121 gatttgaaag ctggagacaa cgtgatcaaa tatggctatc caatcggtca tgtgacgaca
      181 gacgtaactg ccggtcaatg gctgcacact cataatgtaa aaaccaatct ttctggtgaa
      241 ttggattatc gatatgaaaa agatgtccat ccaagtcatt acctttttga gaaccgaaca
      301 tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc gtaatgattt gtttttagta
      361 cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg tcaaacagtt caaggaaaaa
      421 catccagatc tgggacagtt tgaccatatt acgattttaa agcatcctta tggctgttcg
      481 cagcttggaa aagatcatca aaatactcga gaaattttag cggacgcagt aaatcatccc
      541 aatgccggag gagttttagt atttggatta ggttgtgaga acaatacagt tcctgaattc
      601 aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat ttctagtggc tcaagatgtt
      661 tacgatgaga tcgaacaagg tgtggctcta ttagaagagc ttttaacagc ggcggaaaat
      721 gaccagcgca tatcagtccc gctctctaag ttgaatgtcg gtttgaaatg cggcggttct
      781 gatggtctat cgggaataac tgctaatcct ttacttggtg cattttctga ctacttgatt
      841 gctcaaggag gcagcacgat cttaacagaa gtgccagaaa tgtttggcgc agaacaagta
      901 ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa tcgttgactt gatcaatgac
      961 ttcaaacagt atttcttgtc ttacggggaa ccagtttacg aaaacccatc tcctggtaat
     1021 aaagcaggtg ggatcactac attagaagat aagtctttag gctgcacgca aaaatcagga
     1081 agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa tcagaaaacc agggcttagt
     1141 ttattgcaag ctcccgggaa tgatcttgta gctgcctctg ctcttgcttc atccgattgc
     1201 cagttggtct tgtttaccac aggaagaggg actccgtttg gctcatatgt cccaacggtc
     1261 aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc attggatgga ttttaatgca
     1321 ggcgtcttat tagaacaacc aatggaagta atcttagaat cgtttgttca aaaaatcatc
     1381 gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg acgttcgaga aattgccatt
     1441 tttaaaaacg gagtaacact ttgattttca taaaacagta taaaaagaaa gaaggaagat
     1501 ttatgttttt atctgatgat tttttattaa aagacgattg ggcgaaaaag ctttaccatt
     1561 cctacgcaaa aaacatgccc attattgatt atcactgtca cttagatcca aaagaaattt
     1621 acgaaaacaa caatttcagc aatctgacag aagcttggtt aggaggcgat cattacaaat
     1681 ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat cacaggaaat gcttctgatt
     1741 ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat tatcggaaat ccgctttaca
     1801 gttggtcaca tcttgaattg aagcgtttct tcggtattga cttgttgata aatgaagaaa
     1861 atgcagagaa aatttggaca caagcaaatg aaattttggc agctcctgat tttcgccgtc
     1921 gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac aacagatgat cccgcagacg
     1981 acttgcacta tcatcagttg cttgctcatg aagaaccaga tttgaaggtt ctaccttcat
     2041 tccgtccaga taaagcactc aatattgaaa aagaaggatt taatgcatgg gtcacactac
     2101 tggaagaagc agctgaccaa acgatcacca cctatcaaga actgatagaa gtcttagggc
     2161 agcggataga atacttccat caacatggat gtcgtgtttc cgaccacgct ttagacatct
     2221 tacgctataa agaagcggat gaaacagtat tagaagagat ttttcaaaaa gcacgagcta
     2281 acaaactatt atcttcagat gaaatcgatg cttatcgtac agaaacactg atacatttga
     2341 cccacttcta tcattctcat aattggacga tgcagctgca catccatgct tatcgtaact
     2401 gtaatacaca aatgttggaa aaattagggc ccgataccgg ctatgatgga atgaatgatc
     2461 tttctttgac tattcctctg caaaaattat tgaaccgtgc agaagaaacg gatcagttgc
     2521 caaagacgat cctttattca ttgaatccga atgactatcc tgcgatttta gctttgatgg
     2581 gctgtttcca aaaagaacag aatggaaaac tgcagttagg ttctggatgg tggtacaacg
     2641 atactcgagc tggtatgcgc gatcaattga cacaatttgc tgatggtagt gctttgggga
     2701 attttgtcgg catgctgaca gattcaagaa gtttcctgtc ctacactaga catgaatatt
     2761 tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat gcgaggagaa gcacctgaag
     2821 atgaacaact tttaggaagt ctagttcaag cgattagtta tacaaatgct aaaaattact
     2881 ttggcttttt tgcctaattg attaatagta acagtaacga atcaaaaaca ggatagtgaa
     2941 ataacggaat ccgtttatcc acgttttaga gaagaagacg gaccaaattt gctccgtctt
     3001 cttctcttgc tgttctgctt cattgcctat aaacctttat gaaagaagag ataacatgcc
     3061 tactatccat attgccggtg attctactgc ggcagaaaaa gatgaaaaca agcgtcccga
     3121 gacaggttgg ggagaaaaaa tctctgctta tttttcaccc gagatccaga tcaataacca
     3181 agcaaagaat ggacgcagca ccaaatcatt tatccaggaa ggaaggttag cccgtctagc
     3241 ttcacagttt cagccgaatg atttcttatt tgtacaattt ggacataacg accagaaaat
     3301 ccaagaagaa agaggtacgc ttcccttcgg tagctattta aaaaatctgc agatattttt
     3361 tgaatgcgca aaaaaagcga acgtccaaat ggtcttactg acatctgtga cgcgacgaga
     3421 ttacttagaa aacggaacat tgaatccaga tatacttgga gactatccaa aagcgatgcg
     3481 tgcatttgct gagaaacacc atattcctct tttagatgtg ttctcaagat cacaagaatt
     3541 gttccagacc ttctcaaaag aagagacaaa aaaattctac ctgcatctta tacctgatac
     3601 ttgtaaaaat tacccagaag gactaaaaga caatacccac tttagtccag aaggcgctga
     3661 taaagttgcc cgaataattg ttgaattgat aaaagaatcc cgtctgcctc ttgctaatta
     3721 tttacaaaaa ggagtttgag atgtttccat taaatttagg catccgtgcc cacgatttag
     3781 atgttcgaaa tcgtgaagaa atcgttcaaa aagtatcaca gcttcacttg tcccatatcc
     3841 aatttgcacc acaaaaagca tttcctgaac aacttaaatt aactgacatg acacttggga
     3901 gtgctgccta ttttggtgaa tacttccaaa aaaacaatat cgaactttcc attttaggat
     3961 gctatatcaa cctatccagc aaaaatcaag aagttcgtgc acaagcattg gcaactttca
     4021 aacagcaaat tgctctgtgc tctgcctatc aagccaagat gattgctacg gaaaccggta
     4081 gtgtctctgt cggttacaca aaagaaaatt ttactgaaga agcttatcag atcgccagaa
     4141 attctatcat tgaattagtc gctcatgcag aaaattttgg cgtaactgtt gctgttgaag
     4201 cagggatcaa tcatccaatc tataactatc agttagctaa acgtttaatc gatgaagtag
     4261 cttcaccaaa tttaaaaatc attttagatt gtgcgaattt gatgcataaa gaaaatcatg
     4321 ctgatcagga aaaagtgatc cgaggtgcct tggacaattt acatggttat attacagcgc
     4381 ttcatctcaa agactatatc atggaaaatg gaaagatccg catggtacct gttggcaagg
     4441 gctgtttaga tttccgcccg gtactgcatt atatcaaata tgagcgtcct ctaatgtacg
     4501 ccactcttga agctactcct gagatttatg ttcctgaagc aatagaacac ttgcagttta
     4561 tttaccaaga ttgttaagga gaagattatg gaggaacgaa tacagaaaag ttatcaaaaa
     4621 tatcatgcaa atgcttttca gacattgctg catctttatc agggaaatta ttggcgtttt
     4681 gccatgtcag gactcttttt tattatcaaa catcttccat cttggttaat gccgatcgct
     4741 attgccaatg taatcaatgc agcttcatcg aaagattctt ctcagttgca ggccatttat
     4801 ttcaacgctt ttttgatggc ttgtctgatt ggtcaaaata tattgacgaa ttattttcat
     4861 gtaaaatacc atagcacttc gatccgtcaa gtagaagctg gactaagagc gacccttatc
     4921 aagaaaatcc aagaactatc tattccttat caaaaagaat tgcaatctgg acgaatccag
     4981 tcaaagatca tccgtgatgt agaagctatc caaacgttgt cctctcaagt atttgtcagc
     5041 ggattaaata tcgctgtcaa tttattcgta gctttaggta tcacgcttta taaaagtcct
     5101 gttgttttca tgtttttctt attgactgtt cctctcgccg cattcgttac tctctttttc
     5161 cgtaaaagga tcagacagtc caactcagac ttcaggcaag aaatggaaga gacttctgca
     5221 aaagtgatcg aagtgatcga attagcaccg attgctcgag ctcatgggtt agaaaaacac
     5281 gaaacaactc gcttaaataa gcattttact caaatctatg aaaaaggatt ccacttagat
     5341 atcctgcaat ctgttttcgg atcagtcagc tgggctagtt ttcagatatt tcagctgatt
     5401 tgtttgattt tcactgggat attagcttta caagggcgca tccaacccgg cgatgtcgtg
     5461 atgtatcaga cgtatttcac gacgatcgtc aattccgttt ctggaatgat cacgctgatt
     5521 cctacgattt caaaaggcat ggaatctatc acttctattg gcgaactgtt gattgctggc
     5581 gatgtagaaa cttatcacgg taagaaaaaa atccaacatt tggatggaaa ttttcgtttt
     5641 gaggatgttt cttttcttta tccggactca ctcaaaccga ttatcgatca tttttctcta
     5701 gaggtgaaag ctggagaaac aattgctttt gttgggccat ctggctctgg aaaatctaca
     5761 ctccttaact tattgattgg atttatccag ccgacttcag gaaaaatttt tctagatgat
     5821 cagcctttgg atgaactgga tatgcgaagt gtgcgaaatt atttagccgt tgttcctcaa
     5881 accactttgt tgttctccgc ttcgatcaaa gagaacatca cttatggctt gaaaaacgta
     5941 tcaaaagaac gtttagatga agtcatcgaa gctgcgcaac tttcttcact gattgaccag
     6001 cttcctgatg gtctggatac cttagtaggc gaacatggca acaagctgtc tggtggccaa
     6061 aagcaacgta tctccattgc acgagcgttg atacgtcagc caaaaatcat tttgctagat
     6121 gaagcaacct cagctttaga taaccagtca gagaaaaaaa tccagcaagc cctgaattac
     6181 ctgacagaaa cacccactac ttttattgtg gctcatcggc tttcaacgat caaagaggca
     6241 gataaaatcg ttgtgatcga tgaaggaaaa atcgttgaaa taggtactta cgaaaaactg
     6301 ttagctcaaa aaggctattt ctaccgcttg aaaaatgaat gacaaaaa
//
LOCUS       DACDCJ010000121         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDCJ010000121 DACDCJ010000000
VERSION     DACDCJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.122"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IV038_002680"
     CDS             725..811
                     /locus_tag="IV038_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8342836.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IV038_002681"
     CDS             complement(932..1621)
                     /locus_tag="IV038_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342837.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IV038_002682"
     CDS             complement(1628..1906)
                     /locus_tag="IV038_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342838.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IV038_002683"
     CDS             complement(1866..2780)
                     /locus_tag="IV038_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP8342839.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IV038_002684"
     CDS             complement(2762..3142)
                     /locus_tag="IV038_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP8342840.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IV038_002685"
     CDS             complement(3592..3879)
                     /locus_tag="IV038_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8342841.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IV038_002686"
     CDS             complement(3899..4123)
                     /locus_tag="IV038_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP8342842.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IV038_002687"
     CDS             complement(4763..5296)
                     /locus_tag="IV038_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8342843.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IV038_002688"
     CDS             complement(5317..6159)
                     /locus_tag="IV038_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP8342844.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1041 g         2348 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtacactatg ccaagtagcc
     4321 cttgtagcaa tcgatacagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDCJ010000122         6118 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDCJ010000122 DACDCJ010000000
VERSION     DACDCJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.123"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(20..706)
                     /locus_tag="IV038_002689"
     CDS             complement(20..706)
                     /locus_tag="IV038_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342845.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(712..1383)
                     /locus_tag="IV038_002690"
     CDS             complement(712..1383)
                     /locus_tag="IV038_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP8342846.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(1376..1717)
                     /locus_tag="IV038_002691"
     CDS             complement(1376..1717)
                     /locus_tag="IV038_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342847.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(1895..2587)
                     /locus_tag="IV038_002692"
     CDS             complement(1895..2587)
                     /locus_tag="IV038_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP8342848.1"
                     /translation="MLKNKEAVTTSLQVAETFRKEHRAVLKAIDDLKDGLAQKYADLF
                     WEDVYIHPQNKQAYRIVYMNRDGFTLAVLGFNNTQKVLEFKLKYIEAFNEMEKAIRKN
                     TAFDTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNTKVMEVLGGKES
                     RAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQL
                     NRQQRLFGDENE"
     gene            2653..3435
                     /locus_tag="IV038_002693"
     CDS             2653..3435
                     /locus_tag="IV038_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342849.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            complement(3617..3937)
                     /locus_tag="IV038_002694"
     CDS             complement(3617..3937)
                     /locus_tag="IV038_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP8342850.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            complement(3953..4156)
                     /locus_tag="IV038_002695"
     CDS             complement(3953..4156)
                     /locus_tag="IV038_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8342851.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(4171..4947)
                     /locus_tag="IV038_002696"
     CDS             complement(4171..4947)
                     /locus_tag="IV038_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP8342852.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            5246..5566
                     /locus_tag="IV038_002697"
     CDS             5246..5566
                     /locus_tag="IV038_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342853.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5584..6015
                     /locus_tag="IV038_002698"
     CDS             5584..6015
                     /locus_tag="IV038_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8342854.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         1784 a         1139 c          962 g         2233 t
ORIGIN      
        1 tgttgtcagc caatattttt tatcctccta ttctaagttt cttaattgtt tcctggttta
       61 acttgatccc tttgatttga tatttatttt tgaaattgat cacacctatc ttgtgtttct
      121 ccgtgtgatg gattctgcag agtgctgcaa atgtgtactc tgaatgatca acttctttgc
      181 gctttcgtct tcctagcgct ttgtcaaagt gatcgatatc agctcctgtt ttgccacaga
      241 tgcaacagac tctttttgtg atgcatttgt agaagtaata ttcttgattc gctggtaaaa
      301 tctcatagcc ttctttgaaa ggaatatgat gttcaaagat gaaatctaag atgatatttg
      361 ctaagacgtt agcatcactc acagtcgtat tcgattcatc tttgaggctt attttgcgcc
      421 ctgtgacacc ttcaaagcgg aagtaaaaga attccttcca gaagtccgtt ggcatgcctg
      481 tatcgatgaa aatatcgcct atgagtgcat agatgaagtt tcgttgctgt actgtgaaac
      541 gtctaggatc aataaaacga atttcaataa ctcgatcacc atcgtagccg tcatacatcg
      601 tctttagtcg atcaatgttc acttcctcat tgatggttgc acctatgtct tttcctttga
      661 actttttcag aaccgctgaa tatgaatcga ttaatggttt aaacactcat atcacttctt
      721 atctaattct tttctcttag ctgctattgc tcgctccatc aaggcacatt gctcatagct
      781 taactgttca atagtttcaa cgttatcagc taagagccct aatttatctg tctgctcatt
      841 aacatattcg attaaggttt tggtcatatc tttacccatc tgctcattga aagcttctag
      901 aatcgtctct agcatgttta atttcttcgt atcgattcta ggtggtgttg gaatatcttc
      961 cccttgaaat acatataatc ccagtccgtg tagagccaat gctttcacaa agcatcgctt
     1021 caatgagtta ttgatttgca ttgcatttgg tttaacaact ggttggtttc gataatctaa
     1081 aacaggaaat aattcggttt ccgtgtgtcc tttaaccgtt actgagacag atacataagt
     1141 cccagtttca tccataagaa aaggtttata ttcctcaaca agaaagtctt gatgagttcc
     1201 agaaacaact ctgtagtgtt tatactcatt aatagttacc gttgcctgtg gatcattctt
     1261 tttcataatc tcccatgcgt gagcccaaga taaataatca aaatttcctt ttttcttgag
     1321 tattttattt aacttgcgac taaaaagttt ttcaaaagtc gttgtccctt tgttttcact
     1381 catcaaattc tgcctccatt tcagcaatgt atttcttacc tgatccgtaa taagagatat
     1441 caatcaagtt atctctgtca tactcttcta gcgcatcaat caagccatct tcgatgacat
     1501 agatatattc aggttttttg gacttcctcg ataaatggat aagatagaca tgatcccaaa
     1561 tactcacaaa atttcccaaa tcgtcttgat cacatgctag ttcttcatcc gtcaaaagat
     1621 ttcgtctgat ttttcgatta tttgtttcct tgatattcga tttgccccaa ctaggatcag
     1681 tcaaatattg atctagagtg gaaagttctt tttccatatg ctaaaatctc cttatgatgt
     1741 gttttctttg tgactctttg cttgccggcg gagtcacttt tttatttgtt gccatgcttt
     1801 ttgcttgtca atatgttgtt ggcttaggat gattggttta ttgtgtctcc accagcgatt
     1861 agcaattacc gtcccgattc ttagcgcttc agctctattc attttcatca ccaaaaagtc
     1921 tttgttgtct gttcagttga tcgatttcca tgcgaatcgc agtttctggt aaccacattt
     1981 caataaatga aacagcatca tcgaatctct tacgaggtaa ctcgccatat cttgggattg
     2041 aaaaggtacg tttaaattca gaccaaaatt ttgagaatac ttttttgctg atttcttcat
     2101 aagctcggct ttcttttccc cctagaactt ccataacttt cgtatttcct ttttgcttaa
     2161 tttcaaactc ttgttgtccg ctaattcgca tagtattttt aagcatggaa acatcttttt
     2221 taacatcttt catttcttct aattgataga tcatcatgtc ttcaattgtg tcaaaagctg
     2281 tatttttgcg aattgccttt tccatttcgt tgaacgcttc aatgtatttc aatttgaact
     2341 ctaaaacttt ttgagtattg ttgaatccta atacagcaag agtaaagcca tcacgattca
     2401 tatagacaat tcgataagcc tgtttgtttt gcggatggat gtacacatct tcccaaaata
     2461 ggtctgcata cttttgtgca agcccatctt ttagatcgtc aatcgccttt aaaactgctc
     2521 tatgttcctt tcggaatgtt tctgcgactt gtaagctcgt agtcacagct tctttatttt
     2581 tcaaaattac taattattgc atttttttct ctcccttttg gtataattta ggtaaaaagg
     2641 tggtgtatat aattggatat ttcagatact ccttattatc aaaaattgat agcttcttca
     2701 aaattaatcg gtgaaaccat tgctccttct ctagatgtcg ttaacgctat gcagcctgcg
     2761 atagaacaag ctaaaaaatt aattgcaatt caagatactt ttatagcttc gcaagcaata
     2821 ctcaatgatt ctctcattca aattgtcgag aaacgtaaca aatctattgc taatatgatt
     2881 cctccttctt tgttcgcaat acagaaacaa ttagaatctc caagttttaa tattgctaaa
     2941 aaaatggcag ctactatgcc aaaaccttat ttttctgata tctctaaaat cacagaaaaa
     3001 tttgccgttg acatgtctaa gtacgacttc agaatagggg tagctaccca agaattagaa
     3061 aaagcatttt cttccgaacc ggaagttagt acaaaaaatc tactttttaa agacacttct
     3121 aatccatctg atgagtttga ggctaagttt tttgatatcc ttaatgagca aaggaaagtc
     3181 cttgatgatt ctataaagct ttttgaagaa ataaatacag ccaagaacaa agatataact
     3241 aatgaaccat ctgaaaaaca acaagcttca cctgctttct acaaagataa aatgtggtat
     3301 ttagagcaaa ctggggcagc tttgatagga ctagtagtaa ccgaaattgt aaatataact
     3361 attggagtag atcctaacaa tacaatttca ttagctttat ttttgcgtta tttgttacaa
     3421 tttttaatta attaggttaa gtcagtccct cccgactggc tttttcgctc tatattcagc
     3481 ttcatcaagc cccataaaga tccagaccat atatacaatt gtgccgatta atgcttgtct
     3541 gcttccccaa agacctaaag cgtagataat tagtggtgcg ctgaatacta gtgctctatt
     3601 aaatttaccc atccgcttac ctccttaaga tttccgaaaa atttgtttct aaaaattcaa
     3661 gtgttttact tcttaaaaat agatatgtgt ctcttccttc aactggataa tagacaaatc
     3721 catttttgtt tttctcgata tcgataatat ttctatatct tgggttcttt aaaactctag
     3781 aagtaaacca atcatatttt ctgttaatcc gttctagcac ttctggcaac gtcatccatc
     3841 taccagtatc atcagctttt tttaactcct cataatccac ttgggagata attacatagc
     3901 cttctggaat tgggattttt gcttctagat attgcattag ctgttcctcc ctttacaatt
     3961 cgtacatagt gataatcgaa tctataattc tgtttgcttc tgcagaagtc tttttaccgt
     4021 ttaaaattaa agataagtag cttttgctaa ttccaaatct ttcagcaagc atggtgtaag
     4081 ttaagaactt tgaactttcg acatattctt tgattttttc tctatctcgt tgagtgattt
     4141 ctgcaatgtc agtcatacta aaactccttt ctaaccaatt tcctctaaat ccatttgagg
     4201 gtaatatcct tcttttttta gtaattcgta gataaataga cgccctttct gtgtccattt
     4261 ggtattcatt acaattttag tgccaccatc ggctttcggg atctcagttg tatgagattt
     4321 tgtgtatcct tggttcatgt gttttttgca caataaccat tggttaccga cttttttctg
     4381 aatacctagt ttatgaagta atttattcat ctgttgtgga gacatcccat aatctgctgc
     4441 aatctgacta attgttactg aatctgtaga agataatatg ctatctaaat aggagatttt
     4501 aggttcgtac tctgcaatct tttgttctgc gattaatctt ccagttcttt cttcttttag
     4561 ttgagttgct aattgaatga ttgtatctgg attaagcaaa gcttcttcta ctttttctgg
     4621 agttagataa cctccatgtt ttctaattgc tggcaacact tcacttgtta cccaacgttt
     4681 aaattttttg gcagagggaa gttttgattt taagattaaa ctgtacaaac cagattcgtt
     4741 gatgattgtc atctctcttg attgacctga ggtcgtgatt cgcgacccca tcttatcttc
     4801 caaatctaca tgccgcgaca aagcatcttt agtgtttgaa taacccaaaa cactcgcaac
     4861 gtctttgcct acaaaatatg gttcatcatt tactaaaatt gtccgaactt cgttttgttc
     4921 gaaattaaaa atttgcggtg tgttcatatt tctcattcct ttcttttata ttcgtaaaca
     4981 aatttaacaa cttttttcta aattcaattg acagtatctg agtaatactc tatactaaga
     5041 gcataattaa ataagacata aaaacattga ttttaaagct ttcttggcgg tctgcattta
     5101 catatcaata gtgttttttg ttgtcttttt agttgttaaa tctgtttaca agatatagta
     5161 tagctcatta ctcagaatta gtcaaattat ttctgcgtat ttttctaaac tttatttgta
     5221 aacatttaga aaggttgttt tatcgatgaa tacctatgaa ataataaaag acttggcgaa
     5281 gaggaaaaag atatctatac ggcagttaga aattaatttt ggatattcta acggttatct
     5341 agcaaagtgg aaaaataata ctcctaattc agatgaatta agtcggattg ctgactattt
     5401 cgatgtctct gtagactacc ttctaggtag agaagaaaga gaaaccccta aacatgtgga
     5461 tttatcagaa gacgatactg ttttttcttt tgatggaaaa gaaatatcta aggagacaat
     5521 gcgtaaagcg attgcaattg ctaaagcttt agaggaaaat gaatagttgg agtgatggtt
     5581 tgtatgtatt tgaaattgaa agaaatgttg agcgaataca atttgaaagt agtttatatg
     5641 gagatgaaag agcctggttt ctactatcca aaacccagaa tcattttttt gaatgaaaat
     5701 ctatacggtg aaactgcaga agcctttcat ttatctcatg aacttgcaca tttcagcgcc
     5761 tctcattttg aattttcagt tttgtacgat acttctacaa cttttcactc aaaatttgaa
     5821 acagaagcag ataaaatagc tattctaatt ttattaaata tctatattga gaacgaactg
     5881 actgatgaat ctcagtttaa tctcgaaaaa tttatggagt attattctat tcagaataag
     5941 ctcagataca cttgttatgc tgtctgccaa tgctatttta agaaaaaata ttcttacgca
     6001 aggcaatatg tatgaataca tcaagaatta ttaaaaaaac cgatttcagc aaaacattaa
     6061 aaaagcccgt gctgcaacac gggctctttc ctcatatatg agcttctaca aaaaaaat
//
LOCUS       DACDCJ010000123         6065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDCJ010000123 DACDCJ010000000
VERSION     DACDCJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.124"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            832..1566
                     /locus_tag="IV038_002699"
     CDS             832..1566
                     /locus_tag="IV038_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8342855.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IV038_002700"
     CDS             1582..2751
                     /locus_tag="IV038_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8342856.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IV038_002701"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IV038_002701"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP8342857.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IV038_002702"
     CDS             3841..4767
                     /locus_tag="IV038_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP8342858.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IV038_002703"
     CDS             4786..6036
                     /locus_tag="IV038_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8342859.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQAITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1245 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattta
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagcaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DACDCJ010000124         6037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDCJ010000124 DACDCJ010000000
VERSION     DACDCJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.125"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IV038_002704"
     CDS             <1..376
                     /locus_tag="IV038_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342860.1"
                     /translation="EWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTP
                     EEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIV
                     KAKVSKRFIFNPKLEIYSVVIE"
     gene            701..1318
                     /locus_tag="IV038_002705"
     CDS             701..1318
                     /locus_tag="IV038_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8342861.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1359..1658
                     /locus_tag="IV038_002706"
     CDS             1359..1658
                     /locus_tag="IV038_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342862.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1692..1859
                     /locus_tag="IV038_002707"
     CDS             1692..1859
                     /locus_tag="IV038_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8342863.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1904..2509)
                     /locus_tag="IV038_002708"
     CDS             complement(1904..2509)
                     /locus_tag="IV038_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8342864.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            2634..5621
                     /locus_tag="IV038_002709"
     CDS             2634..5621
                     /locus_tag="IV038_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP8342865.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            5770..5979
                     /locus_tag="IV038_002710"
                     /pseudo
     CDS             5770..5979
                     /locus_tag="IV038_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2067 a          992 c         1095 g         1883 t
ORIGIN      
        1 agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa ttatctttat
       61 gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag tagattactt
      121 tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa gagtaccacc
      181 ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc caaatgaaat
      241 gtggtttgaa gttacagttg aatcaagcga cttcaaagaa aaaaagaaag aatacaatta
      301 tattgtaaag gcaaaagtat caaaaagatt tatttttaac cctaaattag aaatatatag
      361 tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt taaagaagtc aaattctgat
      421 gtgaaaaata aaatatacca tggaagacat aaaagaaaaa ctggataact agtttattta
      481 atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat ataatcatgc
      541 gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga ttttctttgt
      601 ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt aatctgattg
      661 ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat ttttttatat
      721 ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga aggaattagg
      781 aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta aacgccctga
      841 atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac aatcattaga
      901 tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa
      961 gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt acaagcaact
     1021 agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct atatggccga
     1081 agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg cgaaacaaaa
     1141 gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg cgatctattc
     1201 tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg gcatgaaacg
     1261 aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact tttcttgata
     1321 gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg
     1381 tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt ttggttcact
     1441 ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc
     1501 agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa accgcgcggg
     1561 acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt tatgcgttaa
     1621 ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa tttacaagga
     1681 ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata actagtttta
     1741 atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa agtgtttatc
     1801 aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat ggaacataga
     1861 aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa tcattcgctt
     1921 tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg
     1981 gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt tgcggatctg
     2041 ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt tgagccaata
     2101 atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca atataactta
     2161 gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca gtattaaaat
     2221 ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata aaagcaactg
     2281 tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg atgacgcaat
     2341 caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta tctttaccac
     2401 tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc aattgtcgat
     2461 ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata aaaaactcct
     2521 ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc tatcatagca
     2581 tttttaaaaa taaattttat gtagacttaa aatttacttc atggagggtt taaatggatg
     2641 aaatcaaaag taacaaaaaa gcatttggca tttatttagg agaacctagt acagaacaat
     2701 taaaccaatt tttttatttg catgaaaaag atttagaatt gatggagact atacgacgac
     2761 cagctactag attaggtttt gcactacagt tagtctcact acgtttttta ggaactttta
     2821 tattagatct taccaatatt ccccaaaatg tattgagtta tgtagcaact caactaaata
     2881 ttgatcctgg agaaattatc tactatagca ggagacagac caggtatcag cacacaaaat
     2941 tgataaaatc acattatgga tatcaagatt tcaaccaacc ggaaatagaa acatacctag
     3001 ctaactggct tttaaataga gcgctgtata caacagaatc ttcagatatg ttgtttgata
     3061 tgcttctaaa aaaatgtctg gatgaaaaga ttgttttacc tggaattact actttttctc
     3121 gttttgttgc tagtgcaatt gaaaaagctg aagagcattt ataccgacaa ttgtctttgg
     3181 ttccaactga agatgagaga aagcggcttc tcaatcattt agaattaatt ggaactccag
     3241 tttatggggc aataatcaaa atggacattt tgagaacccc gttagctgac gatagtcata
     3301 aagaaatagc cagaggtttt gaccgactaa agcaatttca agctttctct actgaaaaat
     3361 ggaagatgga atctattcct gaagggaaaa taaaagttct tgcaaattat gcatttaaag
     3421 ctaaagctca attaattcaa cgaatgagtg aacagaaaaa actggcgttg ttagtggcat
     3481 ttattttcat atacaaacgt aaggctatgg atgaacaaat tttagcacta tctaattttt
     3541 ttgaaactgt ttttagacgc gccaaaaata aggaatctaa agaacgtctt cgtaccataa
     3601 aagatctaga tcgagccgca tcgacacttt cagatattgt agatttagta atgtccagtc
     3661 attctgatac taatgaacta tcccttcgcg atcgtatctt agaaaaatat tctgaggaaa
     3721 atgttaccga agctgtatcg caagttcggc tattggttaa aaatgatcgt gaaccaatag
     3781 ccatttctga acttctgaac acttatcgaa agttcagaaa atttattccg gatattttaa
     3841 ctacactaac atttgacagc aatgactacg gtaacaactg taaggaactc tggaccttca
     3901 ttaataatag atttcctaag ccgattactt acaaaatgtt tggagctatt aaacatactc
     3961 ttccaaagaa atggatttat tacattcatg aataccctga acaaactaat aaatgtgtat
     4021 tgattgcagc tgtcgaattg ttaattcaat ctttaaaacg ccatgatatt tatattcctc
     4081 agagtgtctt atataacaat cctatggatt gcttgattga tgagaaaact tgggttcaac
     4141 aaaaatacgt tttattagaa caactagatc ttccttcttc agcaaatgaa atgtctttat
     4201 ggttaaagaa cgatttggaa ctgtcttata aagaagcgct aaatcgttgg cctacttcta
     4261 aaatggccag aatagaaaat gcagatcgcc tggttgtctc tagacttaaa aagacatatg
     4321 aaaaaaagga agataagtct tttagacgtc gcattcagca gttacttcct aaaattgatt
     4381 tgtcagattt gttattggaa gtaaaccaac agttaaatct tactcaatca tttaaccact
     4441 taagtgacaa ggacaccaaa atgaaagact tggatattag tttattagca gttcttttat
     4501 cagagtcctg caatattggt ttttccccgg tttcaaaaga aggatttgat tctttaaagt
     4561 atgatcgtct tgtctatgtc aatcatcatt atattcgagt ggatacactt tcaggagcta
     4621 atcaaaaaat aatacatgca cacaggaaac ttgccacctc taagatatgg ggtgatgggc
     4681 atatggcttc agctgacggt attcgttata tcacaccaca acgttcccta tattctagaa
     4741 gtaatccaaa atattttggc cgtggtcgtg ggatcacatt ctacaatttt gtctctgatc
     4801 aatatatagg gttccacgga atggtcgttt cagggacact gcgagattcg ctttaccttc
     4861 tagaaggatt cctcaatcaa cccagcgaat taactcctaa tcagatcatg actgatacag
     4921 ctggttatag cgatatgatt tttggtctct ttggcttatt agggtttcaa tttagtccaa
     4981 gaattgccaa tgataaagga accaaacttt ggcgaatcga cgctaacgct cactacgggg
     5041 aacttgatac gttgtctcaa aattcaatta atatggaact gatttatacc cattgggagg
     5101 acattcttag ggttgcaggt tcgttgaaat ctggaaaagt gaatgcaaca gaattaacga
     5161 aatctcttca acggaatggg caacccacgc ctttaggaaa agcaataact gagtatggaa
     5221 aagtttataa gacgaaacat cagcttcgtt atatttctga cgaaatatat gcacggcaaa
     5281 ttttagaaca attgaataag ggggaagctc ggcatgcctt atgccgtaat attttttatg
     5341 gacgaaaagg aaaactctac cagacttatt atgatgggat ggaagagcag ctcaatgcat
     5401 taggattagt tactaatgcc atcatatatt ggaactcact gtatttagaa aaagtatttg
     5461 aacaaatgaa agctgaagga ttcgactgtt ccgaagaaat gatacgcagg caatctccac
     5521 taattagtga tcacatcaat tttgttggta agtatacgtt tagatacgaa gctgaattat
     5581 ccaacggtaa gttacgtcca ttgaatgtag aagaattcta gcaatgttcg ggttttgcat
     5641 gctaccgcac tatttcgtat gactgttcca gcgaatcact tcgaatagaa aaagagaaaa
     5701 acacgagaac agcgttaaat caacgtttct tgtgttttta cttttgtgct accgcactat
     5761 ttcgtttcta tgttccatgt acccctttat caagaagcgc aaacacgcca aattgccgat
     5821 tggttgatcg gcatgaacgc aagcccttta tataccttga atttacaaca aaaaggcgta
     5881 caaggtacat tttcattagg acgtgtacaa acaccgactt tatatctcat ttatcaacgc
     5941 caggaagcaa tagaaaactt taaaaaagaa ccttttttcc tgaataattc atagttttac
     6001 taatagtctt tcctaacatt gatttaatag tctttaa
//
LOCUS       DACDCJ010000125         5920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDCJ010000125 DACDCJ010000000
VERSION     DACDCJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.126"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(26..328)
                     /locus_tag="IV038_002711"
     CDS             complement(26..328)
                     /locus_tag="IV038_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342866.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(548..914)
                     /gene="ssrA"
                     /locus_tag="IV038_002712"
     tmRNA           complement(548..914)
                     /gene="ssrA"
                     /locus_tag="IV038_002712"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1085..1858)
                     /locus_tag="IV038_002713"
     CDS             complement(1085..1858)
                     /locus_tag="IV038_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP8342867.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGTGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2002..2352
                     /locus_tag="IV038_002714"
     CDS             2002..2352
                     /locus_tag="IV038_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP8342868.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2333..3049
                     /locus_tag="IV038_002715"
     CDS             2333..3049
                     /locus_tag="IV038_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP8342869.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3049..4497
                     /locus_tag="IV038_002716"
     CDS             3049..4497
                     /locus_tag="IV038_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP8342870.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4567..5076)
                     /locus_tag="IV038_002717"
     CDS             complement(4567..5076)
                     /locus_tag="IV038_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342871.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5066..5791)
                     /locus_tag="IV038_002718"
     CDS             complement(5066..5791)
                     /locus_tag="IV038_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342872.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2015 a         1031 c         1151 g         1723 t
ORIGIN      
        1 ggctaccttt tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat
       61 acttcaaata gtcataacgc cgatgatcgc tccgtgcgtc tggcacatca gtcacgatat
      121 caaacaaaaa atacacatct ttcttcattc tggtttttgt tgccggaatt ttaaagtagt
      181 tcttattaga gtaatagaga ttgattaata agctatcttc gattgctaaa aagaaaactt
      241 ctgaatccca caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc
      301 ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta tacgaactaa
      361 tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat atattaaaaa
      421 gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa aatctgcgaa
      481 aataaaaaat gctcaaaccc ttataaaata aggatttaag catctagcga aatctgctga
      541 aatctcacaa tggagacggc gggagtcgaa cccgcgtcca aacacatcgc cacttaaaaa
      601 tctacgttca tagtcaactc atttaaggat tcactttaca gcttgccgag tgacaggcat
      661 tctgtatcgc tagtctgatg ttctcttttt caacttacag acggaaagtt gaaacgtatc
      721 ccactaaagt taggaccctt actcgagcac atgggcgatg ccgagaggat ctacgctaac
      781 tgtttttagg cagctaaagc gtaagagttg ttttcgtttt tagcagttat atttaactgt
      841 aacgttttta cgtagacgta acctacgaaa cgcaattcaa gctcgactgt gcctgtcgaa
      901 tccgtaacgt ccccgttagc taacaagggt tacgtgtatt tttcatacaa aattagtata
      961 gcatatattg ggataaattc aataaaaaag cgaatcatat cccattattt atataataac
     1021 tgcccctttc ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa
     1081 gatcttaaat cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct
     1141 ttgtgataaa ggtagaaagt tcttttaatc cgtcataggc gttgcccata gcgattttgt
     1201 tgtcgcaagc ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa
     1261 ctttttccag ctgaagttct ttttgcagac gtttgacacc tgtccctttt gagattcctt
     1321 tcttcactgt atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt
     1381 cgtgatagta ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt
     1441 taaagcctaa aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca
     1501 ttgtttggct atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac
     1561 cgttcaactg tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat
     1621 agatttcttc gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc
     1681 ccgcatcttc catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga
     1741 gaacattgtt ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt
     1801 tcgcatctag taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa
     1861 ataaaactcc ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac
     1921 ctttcgtgaa gaattcctga acatattggt tcagtcaaca atttagagta tactataaga
     1981 agagaattta ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt
     2041 ggcagtcttt ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg
     2101 cctcttgccg caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg
     2161 ttttttctta tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat
     2221 acgaattatt ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt
     2281 atgacagaaa agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat
     2341 tttaatgatt gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga
     2401 aggttgggaa gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa
     2461 cccacaacat tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct
     2521 agtaggaaga gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag
     2581 agattctgaa agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa
     2641 acctttcagt cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt
     2701 ggcagaagca agagaagaag cagtagtttc agatgatcat tttgatatcg agactagtca
     2761 tttcaagatg aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact
     2821 gacgcctaaa gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc
     2881 tcgggagcag ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt
     2941 ggacgcccat atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca
     3001 aactgtttgg ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg
     3061 tatcagcaac tcttagcttt ctgcggaatg gtcgcgttga tcttattgat cgttggagtt
     3121 tcttttacac aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga
     3181 tatgctgaat cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt
     3241 caagatcagg cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat
     3301 gtgaacttta tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta
     3361 ctgaatttta ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa
     3421 ttcacatcga ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca
     3481 tttaatttga atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc
     3541 gataacagcg ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc
     3601 gttgcagtta ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta
     3661 aggaatgcga cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac
     3721 aaagatgaat ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa
     3781 tctcaagcag aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct
     3841 cacgagatga gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat
     3901 gcaatccctg aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt
     3961 ctgatccgtt tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt
     4021 atggtcatta aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa
     4081 gctaaagcag aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt
     4141 tacgctgatt atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag
     4201 tttactgaaa acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc
     4261 acgattgaag atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt
     4321 tattataaag tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta
     4381 ccaatcgtgc agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta
     4441 ggaaaaggaa caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa
     4501 acaagcggta taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg
     4561 attgatttag cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc
     4621 atgagtaaag agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag
     4681 tagctcttgg atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt
     4741 tcgcttggaa agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc
     4801 agctgccgca caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg
     4861 atcttgtttg atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata
     4921 aggaaacatc ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac
     4981 ctcttttttc aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt
     5041 caagtgttca gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca
     5101 aatgatggat aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc
     5161 caataagagt ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat
     5221 cgatggtaaa aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga
     5281 tatcacggat atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa
     5341 gcgcttcata aactgactgg aagcttacga tccacaataa aaccaagata caacaaagaa
     5401 agagaggaaa gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag
     5461 tcaggtgcca attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga
     5521 taaaaaggaa ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag
     5581 ctaattctaa agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat
     5641 cttggacttg attggctaaa gccgattgca aatggtaaag agtcaggcat gctatcaata
     5701 aaaaagcaga aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta
     5761 atcgagcagt agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa
     5821 atgaatatga ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata
     5881 ttttgtaaaa agcaagaggt tgtgaagaag ctgccctatt
//
LOCUS       DACDCJ010000126         5904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDCJ010000126 DACDCJ010000000
VERSION     DACDCJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.127"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            108..1739
                     /locus_tag="IV038_002719"
     CDS             108..1739
                     /locus_tag="IV038_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP8342873.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1742..2821
                     /gene="uxuA"
                     /locus_tag="IV038_002720"
     CDS             1742..2821
                     /gene="uxuA"
                     /locus_tag="IV038_002720"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP8342874.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2895..4322
                     /gene="uxaC"
                     /locus_tag="IV038_002721"
     CDS             2895..4322
                     /gene="uxaC"
                     /locus_tag="IV038_002721"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP8342875.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4406..5053
                     /gene="eda"
                     /locus_tag="IV038_002722"
     CDS             4406..5053
                     /gene="eda"
                     /locus_tag="IV038_002722"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP8342876.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5173..>5904
                     /locus_tag="IV038_002723"
     CDS             5173..>5904
                     /locus_tag="IV038_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8342877.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSII"
BASE COUNT         2022 a          801 c         1232 g         1849 t
ORIGIN      
        1 tggtagaaaa aagcaagttt attccctttt tatattgaca acggttacaa tgggttttaa
       61 aatataaaat gtatcaaaac taacatatca gtttgaggag gttaattatg gtttcgatta
      121 aaaattggca aaattctatc gaagaattca atgaaatgca gattctactg ccgtgttttg
      181 atgtcaaaga agttaagcaa gaaggccagc aacatccagt atggcttcat tttggtggag
      241 gaaatcttta tcgaggattt catgcagaaa ttgctcaatg cttagcagat caaaatgaat
      301 tagaaacagg agtagttgtg tgtgaaacat ttgatgaaga agtagtggaa aaagcttatc
      361 atgactataa caatgacatt ttggaaattg ttatgagtgg agatggtgaa ctcaaaaaaa
      421 gacttttggc agtaacggct gatagtttat attgcaatcc gaagaatata gcggactatc
      481 aaagagtggt taaatatttt gaagatccta aattacagtt tacaacgttt actattactg
      541 aaaaaggtta cagtttaaaa gattctaaag ggcaattctt ttcggtagtc aaagctgata
      601 tcaaatctgg acctagcaat cctcaacata cgatgagtat tgtaacatcc ttgttatata
      661 gcagatttaa tgctggagag ttgccgattg cgatggtttc tactgataat ttctcacaga
      721 atggtcaacg ttttcaagat agtattttta cgattgcaga agcttggcaa aaagaaggat
      781 atgtttctga aaaatttatt gcttatttaa aggataaaga aaaagtgact tttccttgga
      841 gtatgattga tcgaattacg ccaaatcctt cagagaatgt aaaggaagat ctattgatga
      901 tggggctcga ggatttagat atcattcaca caaaaaaaca taccaatatt gctgctttca
      961 ctaatactga ggtagttcat tatttagtta ttgaagatag ttttccaaat ggccggccta
     1021 aattggaaaa ggctggtgtg attatgacag atagagagac ggttgataaa gcagacatca
     1081 tgaaggttac gacaagcttg aatccacttc atactgcttt ggcagtaaca ggttgtctgc
     1141 taggatatca atcaatttca gcagagatgc aggatgatga tttggttggt ttaatcaaag
     1201 gaataggtta tttagaaggt ttaccagtag ttgaaaatcc aggcattatt gatccaaaac
     1261 aatttattga tgaagtaatt aataaacgtt tgcctaatcc ctatattcca gatacaccac
     1321 aacgaattgc aacagatact tctcaaaaag tagcgattcg ctatggtgaa acaattaaaa
     1381 agtatgtcga aaaagacgga aatgcagaaa atctaacatt tattcctatc gctattgctg
     1441 cttggttacg ttatttactg gcagtcgatg atcaagggga aaattttgtg ccaagtcctg
     1501 atccgttatt ggtagagttg caggataaat tatcagtatt gaaattaggt gaacaagatc
     1561 cttcaaagat tcattgtgcg gtacaggaaa ttttagaaaa taaagaaatc tttggtttag
     1621 atttatatca agttggggta ggaaagaaaa ctgaaaaaat cttttgcgaa atgctagtag
     1681 gtcctcaagc agttcgtaaa acaatcaata aatatataac agaagttgga ggaaagtaac
     1741 tatggaaatg acttttagat ggtatggggc aaatgaagaa aaaatctcac tacaagaaat
     1801 tcgtcagatc ccagcaatga aaggaattgt tggaacatta atggatatac cagccggaga
     1861 ggtttggccg agagaacgaa ttaaatcttt aaaagatgaa gtcgaagcca atggacttac
     1921 cttgaaagtt attgagagtg ttaatgttca tgatgatatc aaaattggtt taccaacacg
     1981 agatcaatac attgagaatt ataaacagac gattcgcaat ttggcagaat ttgggattga
     2041 ggttatctgt tataatttta tgccagtatt tgattgggta aaatcggatt tggattatcg
     2101 tttagatgat gggtcttcta cgttagccta tatttcaaaa aatatcccta ctgatccgga
     2161 ggaaattatt agagaagtag aaaattctac taataatttt gaattgccgg gttgggaacc
     2221 tcatcggtta tcacaaatta agcaattatt agaagcctat aaagaagttg atgaagagaa
     2281 attacgagag aactttgcat attttctcaa agcaattatc ccgacatgtg aagaagttgg
     2341 gattaaaatg gcagttcacc cagatgatcc accatattcc attttcggat tacctcgtat
     2401 tgttaaaaat cgtgacgatt tagattggtt gtgtaatgta gtggattctc cttctaatgg
     2461 tattacatta tgtactggaa gtattgcaga acgaccagat aatgatgtct atgaaattct
     2521 atctgaattt tgtcaacggg atcggattcc ttttgcacat gttcgtaata tcaaatattt
     2581 aagtgacaat ggactcgatt tttacgaagc gccgcataaa tcagaatatg gttctctaga
     2641 tatgtataag atattaaaaa caatgtacga taatggattt aatggctata ttcgtccaga
     2701 tcatggtcgg atgatttggg gagaaaccgg tagacctggt tatggattat atgaccgagc
     2761 tctaggtgca gtttatttaa atggtatttg ggaagcgctt gaaaaggggg cgagtaaata
     2821 attatataag ggagtttatt tagaggaaaa attttctctt ctaaaaacta acggaaggaa
     2881 aggagtgttt aaatatgttt ttagataaga actttttatt agagaatgaa atagctaaaa
     2941 gactttttca taaatttgca aaacaccaac caattatcga ctatcattgt catttagacc
     3001 cagcatattt gctagaaaat aagcaataca agaatttgac tcaggtatgg ttaaatgata
     3061 aaggagctgg agatcattat aaatggcgat tgatgcgagc taatggtata gaagaagagt
     3121 tgatcagtgg tccaggtgat gattatgaaa aatttttagc ttttgtaaaa acagtggaga
     3181 aggcgccagg taatcctatt tttgaatggt ctcatttgga attacaacgt tactttgata
     3241 ttgatttgac aatttctgag aagaatgcac cagaaatttg gcgacgtgct aacgaaaaaa
     3301 ttcaaacgga ttcctatcgc ccgaaagaat tgttggagat gatggatgtg aaagccatct
     3361 gtacaacgga tgatcctaca gatgacttgt ggtatcatac tgagctttct aaacaggata
     3421 attctttcaa ggtattacct actttccgtc cagataaggt ctggagtatc aaatcatcag
     3481 gcttttcgaa ctatatttta aaacttagtc aaacagcggc tataccaatc aaagacttta
     3541 atgggttgaa gcaagcattg agccaacgag tggatcattt tcatcaaatt ggctgtcgat
     3601 tagcagatca cggtttgaat acttattttt atgaaatagc agatgaaaaa atactcgaca
     3661 atctgttaat aaaagcttta gcaggaaaaa cggatttcca tttatcagaa attgcacaat
     3721 ttgttacagg tattcaatta catttgatgt ctgagtatac aagatatggt tggacgatgc
     3781 aaatgcattt aaatgcttta agaaataatt caagtatatt aaaggaacaa attggagtag
     3841 atgtcggtgg tgattcgatg ggggatcaag ctagtttgac acaaaatgtt gtagcattgt
     3901 tcgataccgc tgaaaagagt gggtgcttac caaaaacaat tttatattca ttaaatccaa
     3961 ctgattggct tccactagca acagcaatgc aaagcttcca agggggtatg attcaaaaaa
     4021 tccaattggg atgtgcttgg tggttcaatg atacagctga aggaatgcgt caacagttga
     4081 ctacgatggc acaacaaagt ttgttgtcga attttacagg tatgttgact gattctcgaa
     4141 gttttctaag ctatcctcgc catgagtatt ttcgtcgagt attatgtcaa ttactaagcg
     4201 agtgggttaa agcaggccga attccagatg atgaaatgta tcttagtcag atagtggaga
     4261 atatttgtta tcgtaatgcg tataattatt ttaatttttt tgaaatcgga agtagaaaat
     4321 aatacttgaa ataaaagctt aaatttattc tttgtaagtt agtacagcta gacaaagtat
     4381 gtatccataa aggaaggaga tagatatgaa aaaagtagaa attttaagta agttagaagc
     4441 ggctggagtc attgcggttg tacgtgggaa aaataaggat gaggccatta aagcaataaa
     4501 ttcaattatt gcgggtggaa ttaaagggat tgaactaact tttacagttc ctaatgctga
     4561 agaaataatc aaagaaattg ctaaaaaata tgctgatcaa aaagatgtaa taatcggagc
     4621 cggtacggtt ttagatgcgt ttactgctcg tatcgcaatt atggctgggg cagaatatat
     4681 cgttagtcca tcttttgatc gacaaacagc tgagatttgt aatttatatc aaattcctta
     4741 tttaccaggt tgtatgacaa ttactgagat aaaggaggca cttactgctg gcgtagatat
     4801 cattaaacta ttcccaggtt ctgcttttgg tccatccatt gtaaaagcct atcaagctcc
     4861 attaccacac gtaaatataa tgcctactgg cggagtaagt atagagaact tagaagattg
     4921 gtttgaagta ggagttgttg cagttggtgt aggtgggaat ttattggctt ctgttgaaaa
     4981 aggtgatttt gaagaagtaa gtcgagtagc tagtaaatat atagataagt tttatgagat
     5041 aaagaattat taggagaaaa aacaaataat actctgaaat gtaaaaaata aatactgact
     5101 ctttacgctt cagagtatag tacgtacttt catcagaatg aaaacgctta acaacagaag
     5161 ggagagaatt gtatggaaag aaaatttggt ataagagaca aaataggata tatgtttgga
     5221 gattttggaa acgattttac tttcattttt gctagttctt ttttaatgat cttttataca
     5281 aaagtattgg ggataagcgg agctgccgtt ggaacattat ttttagtagc aagatttatc
     5341 gatgcagtta ctgatgtaac tatggggcgt attgttgata gatcgaaagc aactaagcag
     5401 ggtaagttta aaccgtggat tttaagaatg agtggtccag tcgctttagc aagtttttta
     5461 atgtatcaaa cttctatgat ttcagcttct atgagcgtta aggtagtata catgtatgtt
     5521 acgtatatat tatgggggag tattttttac acatctataa atattcctta tggatcaatg
     5581 gcatcagcta ttacttctat tacagaagaa agaacaatgc tatcgacatt ccgaaatgtt
     5641 ggagctactt tagcaagttt ggtcattggt gtattaacac cattatttat ttatacaaga
     5701 gatgattcag gcgctcaaat agttcgtggt ggatcaactt ttacaattgt tgcgggaata
     5761 ttttcgatat gtgcacttat ttgttattta atatgttata aactaactac tgaacgagta
     5821 agaattgacc aatctcaata caaagtttca ttgttaaaat ctttaaaagg gttagttaaa
     5881 aatagggcat tactttctat aatt
//
LOCUS       DACDCJ010000127         5897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDCJ010000127 DACDCJ010000000
VERSION     DACDCJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.128"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(118..1080)
                     /locus_tag="IV038_002724"
     CDS             complement(118..1080)
                     /locus_tag="IV038_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP8342878.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1082..2521)
                     /locus_tag="IV038_002725"
     CDS             complement(1082..2521)
                     /locus_tag="IV038_002725"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP8342879.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2706..4652
                     /locus_tag="IV038_002726"
     CDS             2706..4652
                     /locus_tag="IV038_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP8342880.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4916..5788
                     /locus_tag="IV038_002727"
     CDS             4916..5788
                     /locus_tag="IV038_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8342881.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1866 a         1017 c         1041 g         1973 t
ORIGIN      
        1 gtctaacttt tgggggtcac tacaagttga caactccttt ttgtttacaa atcaattgga
       61 tatctacaaa ttgtattttc taacgaattt tatagacatt aaaaaattaa tttaacgtta
      121 aatacttcct ccagaaagta ttgagattgg taaaatataa tccttatttg tcttttcgcc
      181 tttaattttt ttcagaagaa tatcaacaat aagacttgca atttcatcaa ttggttgtct
      241 tattgttgtc aattgtggga aaaatttctc aataaaagag gtaccatcgt atccaataat
      301 ttttaaatct tctgggatat taagtttaag ttgatgagca attttcatag tgagaatggc
      361 tgttaaatca tcagaaacaa aaataccatc tggtttgtga tttataatag ttgactttat
      421 ttccatttca cgacggatag tagacagatt attaggaatt ttgaaaattt ttccatttgg
      481 attttgaaaa gaaaaaccta gtgcacgtaa ttctgtggga gaatccgtgt tatcatttcc
      541 tgtaatcata atggtatttt gacaaccatt tttttgtaaa gtcttagctg caagttttcc
      601 cccttcaaag ttatctgaag aaacaatgga aacattagga gctaaatttc tatcaaaagc
      661 aacaataggt gctctcacac gctcataatc agcaattcca agattatggc ttgatgagat
      721 tatgccatca acttggttgg cttcaagcat ttcaatgtat tctctctctt tagcaggatc
      781 attttgacta ttgcaaataa tcgttttata tccatgttta aataactcta tttcgagata
      841 ctcaatgagt tcagagtaaa agatattact aatattagga aaaataagac caataagctg
      901 aggtgacttt ccttgaagtc cacgtgctaa attatttggt ttataaccaa gagttttcat
      961 agcttcatga actttttgtt ttgttttttc tgataaatat cccttattat taatgacgcg
     1021 tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca tcagttaatt ttgctaccat
     1081 tttaatattt taattcaaag tattttccag taggttcgcc tgatttgatt tgaataccat
     1141 tttggtgatt ttcagggaat acacgactag taaacacttt ttctccttta ttaataaaaa
     1201 tctcaatgat tgagttatct acaaaaatgt tagcagttgt ttctttagta tcaattgagc
     1261 attcacgaat cgtaccaaag tcagtggcat attgaatgcc tgctttgctg cgatctaaaa
     1321 taatttttcc ttctttggta tctacaatca agctaagtcc atttccttta ctatcagaga
     1381 aaagaagtaa ctccgatttc tgatttggag gaaaagttag ttcaagctca taagtattat
     1441 ttgtttctgc ttttgctgta aattgttcag aactagcacg taatgatgta atagcttcta
     1501 ctggatattg gtaaagttta ccatctttta ttgaaagttc ttttacaaga ctcattgctc
     1561 cctgataatc atacttatca gttggataat cgatatcagg tagtccaatc caactgacaa
     1621 tcagagcacg accatcagga gtattgaatc cttgagtcgc atacgcttca aatccataat
     1681 caagattttg aatttctgaa gcgcccacaa gttttacttt ttctatgtca agcgattgac
     1741 agatcttaaa agtattcgga tagatattcc cataatttag ctcagattta tccaaccctt
     1801 gaggacaata aatgagaaca ggttttttgt ctacaaatac tagatttgga cactcaatca
     1861 tgtattctga accagtgccg ccaaaatcaa ggttgccaac ttcctcccat gtttccacgt
     1921 tattgtcatc cgctttgtaa agtttgataa agcctgattt ttctaaattt tgtgctccaa
     1981 caatcgcata caattgtcct tcataattaa acatttgtgg atcacgaaaa tgatcagtta
     2041 catcagcagg tttatgaata agaacattct cacctttcgt aatgttgtag tctttatcca
     2101 tccatgctcc gatttgaagt gtatcacgtt cccaattttc atttcgaacg tttcctgtat
     2161 agagtaagaa tagattcccc tctatttcat atgcagaacc ggaataagcg ccgtggctat
     2221 ctcgttttgt gtctggaaga agttctgctc ctgttacatg aaaatgaact aaatcatctg
     2281 attcattgtg tacccattct ttcaacccat gagcagctcc aaatggccaa ttttgataaa
     2341 aaagcgtata ttttccgtta aaaaaagaaa aaccattagg atcgtttaac aatcctgttt
     2401 taggttcgat atgatagcta gcacgccaag gtgatcgttt tacattatcc ttaattttat
     2461 ccatctcatc tttagtccaa tcagaataag cacgatatcg tatttctcga ggtaagttca
     2521 tattttactc cttcaattta tctttttaca actagtatgg taaacgtttt ccgttgaaaa
     2581 atcaatcatt tcatttaaaa aaataaacta tatgacaaac gtttgacata tttttaagat
     2641 atgctaaaat aaatttgcat ttcagtgaac cgttttcacg taaaaataaa aaaggagtga
     2701 ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca agcggtcgga aaagataacc
     2761 ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt actaaaagat gacacgaaag
     2821 tgaatcaaaa agctttagac gaaaatccag atgtgaaaag cacatttaaa attgatggac
     2881 aatatcaagt gattatcggt gcaggtgatg ttaatttcgt ttatgacgaa cttatcaaaa
     2941 aaactggact ttcggaaatt tcaacagatg atttaaaaca aattgctagt aaagataaaa
     3001 aattcaaccc cattatggca ttaattaaat tattatcaga tatttttgtt ccaattatac
     3061 ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa ctttttaaca gctgctggac
     3121 tttttggacc aaaatcaatt gaagaaatgt atcctgcgat tgaaggtatt tcctcaatga
     3181 tccaactgat gtccgctgca ccattcatgt tcttaccaat tttagtaggt atttcagctg
     3241 ctaaacgttt tggagcaaac caatttcttg gtgctgcaat tggtatgatc atgacaacac
     3301 cagatcttgg tggctcaact gaatattgga atatttttgg ttatcatgtt tcacaaacaa
     3361 actatgctta tcaagttatt ccagttttag cagctgtttg gatattatct atattagaaa
     3421 aattctttca taaaaaattg ccatcatcta ttgactttac attcacgcca ttattatcag
     3481 taatgattac tggatttttg actttcacag ttattggccc tgtaatgtta ttgttgtcta
     3541 atggaattac agacattatt gtttggctat ataacactac tggatttatt ggaatgggta
     3601 tttttggagg aacatattca cttatagtca tgactggttt acatcaatca tttccagcta
     3661 ttgaaactca attactttca gcgtggacaa atggtatcgg acatggagac tttatctttg
     3721 tagttgcatc tatggcaaac gttgctcaag gtgctgcaac atttgccatt tggttcttaa
     3781 ctaaaaattc taagacaaaa agtcttgctt cctctgcggg tgtctcagca cttcttggaa
     3841 ttacagagcc tgccttgttc ggagttaact taaaataccg ctttccattc ttttgtgctt
     3901 taattggttc aggaattgct acagcagtag caggattact taaagtagtt gctgtttcac
     3961 ttggttcagc aggtttcttg ggatttcttt caattaatgc aacatctatt ccattttacg
     4021 tattgtgtga attgattagc tttgtagttg cttttgcaat tacttacttc tacggtaaaa
     4081 caaaagctgc tggtatcttt aaagcagaag aaattgctga acaaacacca gttaattctt
     4141 caaaagtagc aacaacagat ccaatatcta ctgatgttga atcaacacaa acaaaagttt
     4201 catcagaaac aattgttagt cccatttctg gtaaagtgat tagtctttct tcagtaaatg
     4261 atccagtctt ttcatcagaa gctatgggaa aagggattgc cattaaacca aatggaaaca
     4321 cggtccattc tccagtagat ggtactattc aactcacgtt tgaaactgga catgcttatg
     4381 gtattaaatc aaataatggt gctgaagttc taattcatat tggtattgat actgtatcaa
     4441 tgggaggaaa aggatttaca caaaaagtta ctactaatca aaaagttaga aaaggcgata
     4501 ttcttggagc atttgaccgt tcaatgattg agaaagaagg tcttgatgat acaacaatga
     4561 ttattgtgac caatacttcg gattattcag aagtaaatat agtagctgaa aatacagtca
     4621 ctgagggtac tgatttactt gaaatcaaat aaatatattt ccctgagtaa agatggaatt
     4681 ttctttccaa aatcaaataa tatagattaa tcaaatattt gagaataaca ttttatttaa
     4741 tcttttcata tatcaattgt atgaaaaatt attagaattt taaaaaatga taaaaattca
     4801 aaaagaaaaa atagtagtta tttacttgtc atgttactaa ataaaaaaat aatttaaata
     4861 tgatatatag tcatttattt agtgatttca ttctaaaata aagggagaaa tgtctatgct
     4921 atacggtgca attgaagcag gcggcacaaa atttgtgtgt gccattggcg atgaagaaat
     4981 gacgatcaag gaaagagtga gctttccgac aacgacacct gaggaaacaa tgcctttagt
     5041 cattgacttc ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg
     5101 gcctatcgat atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaatt
     5161 ggcatggcaa aattttgatt tcatcggtac catgaaaaaa gaatttccga tcccgatttc
     5221 ttggacaaca gatgtcaatg cggctgccta tggtgagtat gtcttcggca gtggcaaagg
     5281 attgagcagt gtcgtttact atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga
     5341 agggcgcttt atcgaagggt ttagtcatcc agaaatgggg catatgctcg tcgtccctca
     5401 tccaaaagat gactttgctg gcagctgtcc atttcatggc aactgtttag aagggatggc
     5461 cgctggaccc gccatcgaaa accgcttagg gaaaaaagga caagaggttg cagaagatga
     5521 tccttattgg gaaatcgaag cctcctatat tgctcaatgt gcctacaata caaccttgat
     5581 gttttctcca gatgtgatca tttttggcgg tggtgtgatg aaacaacgcc atatgctgca
     5641 aaacgtccac gacgcatttg caaaattggt caatgggtat gtcgaaacac ctgagttaag
     5701 caaatatatc gtgaccccag cattagaaga caacgctgga acgctcggtt gtttggcatt
     5761 ggctcgagaa gcggtcttac attcataaac gaaaaaaggc tcttcgacat ttagtgttgg
     5821 tagagaaaga tacaactgaa aatttagtca taaccagtta ggcagagtgc ttaactggtt
     5881 tttgttttac ttggaag
//
LOCUS       DACDCJ010000128         5862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDCJ010000128 DACDCJ010000000
VERSION     DACDCJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.129"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IV038_002728"
     CDS             complement(99..1229)
                     /locus_tag="IV038_002728"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP8342882.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IV038_002729"
     CDS             complement(1878..3080)
                     /locus_tag="IV038_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8342883.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IV038_002730"
     CDS             complement(3140..3343)
                     /locus_tag="IV038_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8342884.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IV038_002731"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IV038_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IV038_002732"
     CDS             4880..5692
                     /locus_tag="IV038_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8342885.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1209 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa tt
//
LOCUS       DACDCJ010000129         5844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDCJ010000129 DACDCJ010000000
VERSION     DACDCJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.130"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..308)
                     /locus_tag="IV038_002733"
     CDS             complement(<1..308)
                     /locus_tag="IV038_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342886.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     T"
     gene            complement(461..1372)
                     /gene="vanY"
                     /locus_tag="IV038_002734"
     CDS             complement(461..1372)
                     /gene="vanY"
                     /locus_tag="IV038_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8342887.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(1800..2408)
                     /locus_tag="IV038_002735"
     CDS             complement(1800..2408)
                     /locus_tag="IV038_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8342888.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(2414..3445)
                     /gene="vanA"
                     /locus_tag="IV038_002736"
     CDS             complement(2414..3445)
                     /gene="vanA"
                     /locus_tag="IV038_002736"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8342889.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(3438..4406)
                     /locus_tag="IV038_002737"
     CDS             complement(3438..4406)
                     /locus_tag="IV038_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8342890.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4738..>5844
                     /locus_tag="IV038_002738"
     CDS             4738..>5844
                     /locus_tag="IV038_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8342891.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTL"
BASE COUNT         1702 a         1297 c         1035 g         1810 t
ORIGIN      
        1 gtcgctccaa tagcgaagat aaattgaatt atttcaaaag taagacttaa aaccagtaca
       61 aaagcaaact taggtaaaaa tccgatttct ttaaaattga cattcaaaag caagccaaat
      121 ggaataaaga ttataacatt atctatcatc tctctgaaat tcccagtagc agtaaatgga
      181 gtcaagttaa gacttctttg atgataatta aatactgata aaatattgta ttgtaatttg
      241 aataacacta accagattag tgtcactaaa tataaagcta gcaatcctct agataatatt
      301 tttcccaata taatttcctc taattctttt gtttaagttg tatcattagt atagcaatgt
      361 cctagttaac gtgggtttta aattaaaaag ctaacgttac cccaaaagtg ctagtacaat
      421 aatgctgttc attaatttcg ttggtttcat tccgccatcc ttacctcctt gaattagtat
      481 gtgttgatcc gggaaacact gtcacaatta caccgtctat attgtttcct gatatctcat
      541 aacgaagatt agtcggcaca tgaatggtgg tatttttagt aacaggataa taaaagatct
      601 catatttttc cccatttaca ctaacagaaa tggttttttc ttcttttagg taatccatat
      661 attcctcgag aacgaaattc ttttctttca taatcgcact atgtggtaaa ccaacatagc
      721 gaatatgcca tagttcatat tgaattcctg ttaactctgt tttgtcctct ggataacgta
      781 aaatgaaccc gtatttccaa gcattttctt ctatccactt tccttcaggg gctcgttcca
      841 ttttcgtcaa gcttgatcct acatctagtg ataaacctga attatgctca ctataacctg
      901 ctggtaaggc atactcagcc cccatttctt ggtaaagcac actttgctca tcaaagtctc
      961 gatagccact attaataata aaatgactaa cgccaccctt tacagcatca ttgaccatct
     1021 ctgaaaattt ttgtgctatt tcttttgaca tataaatatt actatcaagc aacccgtatc
     1081 catttattaa ttcgtcatgt ttagataaat tcacgatatc tgacttcaca ctttcttggc
     1141 gaacaggata tttactattg attaatagca gatttccttg ataaacctgt tcttctgtaa
     1201 ttgttttctc ttgggtattt tcagaagtcc cactattttc taaatgttct ttgggatttt
     1261 gatcataatt ttgaaattcg actttttcct gagaaaacag tgcttcatta acgtagtcat
     1321 aacctaagta tattaagaat aacaataaca ataaaaaaaa caacttcttc attttcagtc
     1381 tcctccttaa ctatatagtt ggaagtatga aatatttcta gaatcttccc cttaaacggc
     1441 acaatctcgt ttgagattgt gccgttttgc cccgtaaatt acagtcaatt atttcgccct
     1501 actggattta ctattatcac caatgtagat gaattttcat aagtgatacc catgctgaat
     1561 ttaacgaggc tgaactgcct gttgacaatg ggcggtgtga aataccaccc ctctatgcga
     1621 tcagtcttat tatcaggcta tcttgccgca gcgcacgcag ataatgaccg caatatcgct
     1681 ctgccttaca tcttcaatac tatctactat atataaattc cctaagaacc agttacatac
     1741 gtcgggtttc ctgccgaaag agttagctta tatagtttgt ccgtgcaacg gttaaaagtt
     1801 tatttaacgg ggaaatcaaa atagctattg gggtatggtt cgtctcttaa tacatagtgc
     1861 caccattcga ggctatatgc ttcaaaccca ctgttttcca tgatggagcg caaacgtctg
     1921 cgattttgcg cttcattgca tgatattcca tttgccgcat gatgagagcg ttcatccata
     1981 aaatcaaatc ggctccccat tggtacaagc tcacccgtgt ctaatcgata aagcgtaaga
     2041 tcaatggcac tgccgcggct atggcttgat tttgaagcca cgtatccttt tgaaatcatc
     2101 tcagttcggt caatattggg ataataactt tcctttgtca ggttattttc cggctgtgca
     2161 gcccattgca taaaacagtt tacagcacga ttaggacggt aaccgtccca tagaagcaat
     2221 ccgtaccctt gggtagcagc cagttctttt gccttcaaaa gcgattcagc caactcgtat
     2281 gtccctacaa tgcgatttac ttcataaccg tcaaccggtt ttccggtgaa attatcccaa
     2341 gtggcatatt tagcgtccca acgaacaccg tgtactattt catctaaaaa agtaaatcct
     2401 atttccatgc ttatcacccc tttaacgcta atacgatcaa gcggtcaatc agttcgggaa
     2461 gtgcaatacc tgcagcggcc atcatacggg gataacgact gtatgacgtg aaaccgggca
     2521 gagtattgac ttcgttcagt acaatgcggc cgttatcttg taaaaacata tccacacggg
     2581 ctagacctct acagccgagc gctttatata ttttttttgc cgtttcctgt atccgtcctc
     2641 gctcctctgc tgaaaggtct gcgggaacgg ttataactgc gttttcagag cctttttccg
     2701 gctcgacttc ctgatgaata cgaaagattc cgtactgcag cctgatttgg tccacctcgc
     2761 caacagctaa cgcggcactg tttcccaata ccgcacaacc gacctcacag cccgaaacag
     2821 cctgctcaat taagattttg ctgtcatatt gtcttgccga ttcaattgcg tagtccaatt
     2881 cgtccgcgct attgactttt ttcacaccga aggatgagcc tgaacgcgcc ggcttaacaa
     2941 aaacaggata ggtaaacgta gctgccaccg gcctatcatc tttattaata acccaaaagg
     3001 cgggagtagc tatcccagca tttttcgcaa cgatgtatgt caacgatttg tccatacaaa
     3061 ttgctgagct ttgaatatcg cagcctacaa aagggatacc ggacaattca aacagacctt
     3121 gtatggatcc atcttcacct gacttgccat gcaaagctga aaatgctaca tcaacatggt
     3181 tgatttcata ttcatggttc tttttaacaa gtaatccgtg cattttttta tccggcgaga
     3241 gtacagctga atagcaattg tcgttttccc attccgcgca aggtttttcg cacattttcc
     3301 atacaccaga tttcgtaatt ccaatgtata acggctcgta tttttcttta ttaatgttag
     3361 cggctatctc tattgcagat tttaccgata cgtcatgctc ctctgagcaa cccccaaaca
     3421 gtattgcaac ttttattcta ttcatgctcc tgtctccttt caaaatccaa acagttttta
     3481 atggtttttt caacggtatc acgcaacgct tgctcggtat aataggccgt atgcggtgtg
     3541 attatcacgt taggcattct ttgaagttta agtaaaaatt gattatcaat tggtttttgg
     3601 gtgcaatcag agtagaaaaa ctcttcctct ccttccaata catccaatgc ggcaccgccc
     3661 agtttcccgt tttctaatgc tttaaccaac tcataggtat ctacaagtgg accgcgccca
     3721 gtattgataa gaaatgctcc ttgcttcatt ctctgtattt gttcgtggct gataatatag
     3781 tgcgtatccg tattgagcgg cacatgaagc gtaacgatat cgctattttg cagcaactca
     3841 tcaaacggta catagtttac ctctatactt cggctgcgac tataagccaa cactttacat
     3901 ccaaatcctc gcagccgctc aataaccgct ttgcctatct ggcccgttcc caccacacca
     3961 actgtcatgt cgctgagtac cttgccacgg tcgctgtcca acctgaaatc atgtttttcc
     4021 acagagcgca caatcgattt tacgttgcgt actgccataa gaattagcat catagtataa
     4081 tcggcaacgc tatccggcga gtacgccaca ttgtcgacag tgatgcccat tctcttagca
     4141 gcagttgtat ctatatgatt gcagccgatg cttcgggtag aaatatattt cacaccggct
     4201 ctcttcagcg caagaagaat agaggcggaa atctctgatt tatgtcccac actgatacat
     4261 tgattgaaag gcgcggattt ggcgttggat tccgacacgt tggcgttaat tatcgttgcc
     4321 ataacgccaa agcgaggcga aagagcatgg aatgcatctg cctcatcctg ctcacatcca
     4381 taaacagtaa tgccgatgtt attcataatt aagaccaacc ctttctgtga aaggcaccaa
     4441 ataaaacggg gatgccaatg gtaatcttgt caaatctttc tctgaagaac gaaaacggct
     4501 cgttcttacg gtattaagtc taccgtgtaa gaacgagccg tttcttaata ttaaattgtt
     4561 aatttcctaa gaaaaagtaa agataacctt gagattttcc taaaaaatta tggctctata
     4621 ctttttgatg ttggtggatg acaccagcat cttttttatt ttccaaaaca tatagaaaag
     4681 acaccttgaa atcctttaga attaatttgg cgaaccaacc caaaaggagg aatcaagatg
     4741 tcttacactc accttatcaa agaaacatta gatattctag acttatcagt tacttttaac
     4801 gaaaattgtc tcacaaaaga aaaatacaaa gggcaaatct gtcatattta tcgtggaaac
     4861 ttgatttata cagcccaaga atgcatccac tgtaaacacc agattgcttc agatattgtg
     4921 cgttggggta caacaaccgt ccggttgtta atgaacgatg tttctgaata ccgtacctac
     4981 ctcgaattga aaaagcaacg cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat
     5041 acttctgttg cgaaaaaaca ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact
     5101 cgtctgaaaa aaaatacctc aatgacagag atcgccgctc agaaaaatct atcggtctct
     5161 tctgtttacc gtattatgaa gcgattttat cgtccgttga atccttttcg gacaccgtta
     5221 cctaaggtac tttgttttga tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt
     5281 ttcatcatga tggatggaca aacacagcgc ttattggaca ttgtcgagaa ccgacaactg
     5341 ccttttttaa aacgttattt tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg
     5401 atcgtgattg atatgtacgc tccttatgta tcgctcgtta aaaagctgtt tccaaaagca
     5461 cagctgatta ttgaccggtt tcacatcgtc caacatattg gtcgtacctt tcgcaatcat
     5521 cgaatcaaag aaacgaacca actattaaaa agcaaagaac aaaaacacta tcaattaggt
     5581 aagcaattaa aaagatactg gaaacttctt caaaaagatg aacgcaagct tgattatact
     5641 cgacgccttt ggcgacctgg cttcaaagct catttaacag agaccgatat tgtggatcgc
     5701 ttactgaaag ggagccctgc tttgcgtgta ggctatcagt tatatcaaga ctttctttac
     5761 gctgtaaagg aacgagacta cgtatcgttt gaagaactac ttaccaataa tatcatgctt
     5821 cccgaaggct atcagacgac cttg
//
LOCUS       DACDCJ010000130         5720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDCJ010000130 DACDCJ010000000
VERSION     DACDCJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.131"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..770)
                     /locus_tag="IV038_002739"
     CDS             complement(<1..770)
                     /locus_tag="IV038_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAP8342892.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHFFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
     gene            864..1607
                     /locus_tag="IV038_002740"
                     /pseudo
     CDS             864..1607
                     /locus_tag="IV038_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
     gene            1619..2050
                     /locus_tag="IV038_002741"
     CDS             1619..2050
                     /locus_tag="IV038_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP8342893.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            2064..3509
                     /locus_tag="IV038_002742"
     CDS             2064..3509
                     /locus_tag="IV038_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAP8342894.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            3795..5420
                     /locus_tag="IV038_002743"
     CDS             3795..5420
                     /locus_tag="IV038_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8342895.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         1857 a          882 c         1163 g         1818 t
ORIGIN      
        1 atattacggg aaacgtgaac acagcaatgt tggtatttag ctttaggata aatctggtga
       61 atcgtatctt tcatgccttt taagccatcg gtaataaaca gtaagacatc atgaacccct
      121 ctggagtaaa tgtcctgtaa caactcattc caaacgtagg tcgattcagt cggagcaatc
      181 gcataactta gtacttcttt agtgccgtct tctcgtatac caatggcaat atagattgct
      241 tctttggaga cggtttgccg ttttaatgga atataagtag catccatgaa aatagcgaca
      301 tacttatcat ttagagttct aactttaaat gcatttactt cttcagtcag cactttagtc
      361 atgttggaca tggtttgggg cgtgtagtga tgcccataca ttttctcaat caagtctgcg
      421 atttccgaca tggtaatacc ttttcggaag aagtgaatga cggtctcctc taaggtatca
      481 ttcgtccgtc tataagcagg aacagtctgt tgcttgaact cgccgttgcg gtcgcgcgga
      541 atctggagat gaagttcccc gtactcggtc ttgaccgtac ggtcatagga gccgttacga
      601 gaattacccg tgttaaaacc aatgcgatcg tacttttcgt aatcgaggaa agccgttaac
      661 tccgttttga gaagcgtatt gacagctttt tccaaatgga cacggaataa ttcattcaaa
      721 tcgcctttgt tcgctagagt ctttagaatt tctgtagtaa aatcgttcat atggaagtcc
      781 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      841 ttacacaaaa tattttacac tctcaaattt attctttgct gtcagaaaaa gaacaagaga
      901 attttgatga atatgcatat tttgtgaata cggcaatcga tcgacaatct ttaggaaaag
      961 ttcttggagg aaaaatgatc tctgttgtcg aaccttatta tgaatggata atggatcgac
     1021 agcagttaga tccaactaca tcttttgata tgaaaaatgt aaatttggtt gatgatatgg
     1081 agccttacat tgagcgaaag ctttatatcg ttaatgctgc tcatgcggca gtagcttatt
     1141 taggcgcact gaaaggatat aagacgattc aagaagcaat ccaaaatatg gagattttca
     1201 atctagtcaa gcaattttta atggagaata tagagtactt tgtacaaaaa tatcattttt
     1261 ctagagaaga attaacatct tttgtggaaa aaacattaaa ccgtcatggg aatcagaaac
     1321 tctccgatga aattactcgt gtaggaagtt cccctattcg aaaattaggc cctaatgatc
     1381 gtttaattgc tcccatagta aaactagatt catatcattt accttacgat gcaggaataa
     1441 aagtaattgc tgcgggatat cattatcgga atcttgctga tccagaagct gaacaactac
     1501 gtcgaatgat tgtaaatgat gggttgaaag caacgattaa aaagatttct cgtttggaag
     1561 gtaagctgct gaatgaagtt gtcgctgctt atgagtccca gatttagagg aggagaatat
     1621 gctaaaagaa caattgtcag gaaatacaat gtttatggat gcgatcgatt catggcaaga
     1681 ggcaatccga gtaagtgcgc gtcctttgat aggaaaggga ataatagagg aaagctatgt
     1741 acaagcgatg atacaaaatg tgttggataa cggaaattat attatattac tgccactagt
     1801 agcaatgcct catgcgcgtc cagaatttgg gagcaaaggg gtcgggatga gctttttatg
     1861 tttagataag ccagtcatgt tcccaagtga agaaccagta aaattcttct ttaccttttc
     1921 ttctgattcg ccaaagggac atttagactt aatggccaat cttggagaat tgctgagtga
     1981 tgaaaaaatg tatcaaaaat tattcgatgt gaagacagaa aaagcattgt tagatttgat
     2041 aaagaagtaa aggagagaag aaaatggagg tagcaaacaa aaaaaaacga gtctccatgc
     2101 gagctaaagt tcaagcgatt ggcggtttct taacaaatat ggtattgcca aatataggcg
     2161 catttattgc atggggaatt gttactgcat tattcattcc aactgggtgg ttcccaaatg
     2221 aatctctgaa tgaactcgta agtccgacag tcagctatct attgccgtta ctacttgcct
     2281 atactggtgg tcggatggtt ggtggtacac gtggagctgt tttaggtaca atcggaacaa
     2341 taggtttgat tattggtgct gacataccaa tgttcatggg agcaatgatt atggggccac
     2401 ttggtgggtg gatgatgaaa aaaacagatg attttttaga tgggaaagtt cctgctggtt
     2461 ttgaaatggt cgtcaataat ttttctatag gaattattgg tttcatactg atgtgccttt
     2521 cttatctggt agttggacca ataatccaat cagctaatca atttgtaacg agtgcaattg
     2581 aagcattagt ttcaactggt tttttaccga tactgtcctt aatcaacgaa ccggcaaaag
     2641 tcttattttt aaataatgtt attgaccaag gtgtttatta tccattaggt atgcaacaaa
     2701 cattagaggc aggcagatca atttatttca tggtagcatc aaatcctggc cctggcttag
     2761 gactattgtt agcttattct ttatttggta aaaaggcagc aaaacagaca gcaccaggtg
     2821 caatcatcat ccattttttc ggcggaatac atgagattta ttttccatat gtattaatga
     2881 aaccactcac tatattatct atgattgctg gtggaatgac aggaatcatc acttttgaat
     2941 tatttgatgc gggcctggta gcaggtccaa gtccaggttc tattttttct tatttagctc
     3001 taacgcctcg gggaaatttt ttgggtgtca tggcgggagt ggtcacagca acactggttt
     3061 ccttttttgt aacatcagta attttaaaag cagataaatc aacagatgaa gacagaaatt
     3121 ttgaactatc agttcaaaaa tcgaaaacga tgaaatcaga aggaagaaaa ttattaaaag
     3181 aagaaaatgc tagagaaata tgtaccgaga aaaaagagat gaataaaatt tcctttgctt
     3241 gtgatgcagg aatgggaagc agcgcaatgg gcgcaacaat attcaagaaa aaattgcaaa
     3301 aagcaggtat tactgatgtt gagataaaaa attatcgaat tgaagaggta ccagaagata
     3361 gtgatttggt gatcgtacat caagatttgg aggaacggac tagaaaagca catcctaaca
     3421 tgaaaattgt gacattaaag aattatttgc aagatccagt aatcaatgga ttagcagaag
     3481 aaatcattgg attaaaaaca gaagactaat ttatgggaat ttttgattaa cctttattaa
     3541 aattattttg cctaatataa aaaggttgtg acatggcttg tgtcgcaacc tttttttgtc
     3601 gcacaaataa ttaatcggat attttatgtt catatttttt gtgatcaacg agaactgatg
     3661 gggcaaagat acattttaga tacttttaac tgttaattta caaaacaagg tgaaacgaat
     3721 agaaaaatga aaaaatcgat acggaagaat atgtttgatt aaaacatgca tgaagagaag
     3781 gaggttgatg atacatgaaa aaaatatatt caaaggaaat cgattgtttt agaggcattg
     3841 ctgttttagc agtgatattt tatcatttgc caaatagttt gctgccaggc ggattgcaag
     3901 gagtaacgat cttttttgtg gtttctggtt ttttaatggc taaaaatacg ataagagatg
     3961 aaaaaaacag acaattcagt tttttacatt tttactataa gcgtatcaaa cgtatttatc
     4021 catttttact gttttctttg gtcgtattca ttttcatact agggattagc aacattcgat
     4081 tgataggaaa cattcgtagt gagctaccca gtttgatttt tggctataac aattggtggc
     4141 agctgaatca aggagtatct tattttgact cttactttaa tgcttcttta tttaaacatt
     4201 attggtcgct ttcagttgaa ctacaattct atttgatatg gccatttttg tttattgtca
     4261 ttaagcgtat gagaagaaaa caagtgtttt acttaatcta tacattgatt tttgtttcta
     4321 ttctttttag tttgttttta ccttcaacta aggcatatta tcatacgata gctaaacttt
     4381 ttccattttt attaggagtt tgggggtatt ttaatcgaat aactattggc cgtttctttg
     4441 aacaaaacac ttttagtaaa atctggcttt tattactagc tagtctttgt ctgattctgt
     4501 tcccgatttt tccttataca ctaaatgaac tactgatcag tatttgtttt gctctattgt
     4561 tagccagtgt cgatgatatg aacattgcaa aaaagaccaa aatcgctagt acaaaacttt
     4621 cttttgtagg aaaaatcagt tatgaacttt atttggtgca tttcccagta ttgattgctc
     4681 tgacaaatct attcaaacat caccattgga aatatttgga tatcgcaggt ctattcactg
     4741 tgtttttatg tacattactc atgattactt gctttagaaa aggagtgcaa aagatgagat
     4801 cacccaaaat ttggacagct atggcactaa tgataatcgg agtttctgta ctggtattac
     4861 gtgctcctgc cagtcgactg accgctgacc aaaatcagtt gcaaagttta ttaaaagaaa
     4921 actctagtaa agcaaatgca ggagaaaatc tgttgtcaca agagaagaaa atattattta
     4981 tcggtgattc tgttatgctg ggggcgtatc aggaactaaa ggagacattt aaaaaagaag
     5041 cattggtaga tgcaaaagaa tcaagacaaa tcactgcgtt accagaaatc ttaaaggagc
     5101 ataaagattt agtttcttac caacaaatag tgatcgggtt aggaaccaat ggtgtgataa
     5161 cagatgaagc tatagaacaa acaatgtctt tgctgaaaga taaaaaagta tactgggtaa
     5221 atataaaagc accaactggc tggcaagata cgataaatgc cacattagct acattgccaa
     5281 atcagtattc taatataacg atgatcgatt ggtatagtga gagcaaaaat catcctgaat
     5341 ttttttatga tgatgaaacc catttgaatg aaagtggacg tacagcttat gcaaagtata
     5401 ttgcttcagt tattcaataa ggtagtgatt acgtgtgtta accttacttt ttcctaatag
     5461 gtgaatcaat ttaaataaaa aatacttcat atgataaacg gtgtttaaga ggaggagctt
     5521 tttaatattc gtggtaaaaa agagatgggc gaggtcgtta actgagagct tcttggaaag
     5581 aaatcgcttt ggatttatcc tctttgttat acagaacatg agcgagacga caatactagg
     5641 atgtatgttc tgatcgaagt tttatggtta caagcagcaa tcagcttaga taaaattaag
     5701 gatcgtagga accaagtaaa
//
LOCUS       DACDCJ010000131         5686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDCJ010000131 DACDCJ010000000
VERSION     DACDCJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.132"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(58..2421)
                     /locus_tag="IV038_002744"
     CDS             complement(58..2421)
                     /locus_tag="IV038_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAP8342896.1"
                     /translation="MATVILAEKPDQAEKFAFALADKKIPKSGGKFEFQSEVFGDTIV
                     TWGIGHLVGLSMPEKYEWLPNKEKWDLANLPFLPKMDELKYEVSKGKSQQFTVVKSCL
                     ENADQIIIATDPDREGENIAYNIFKLCKQSIFKKPMKRLWINSLVKSEIIKGFKNLRN
                     AEETISFFEEANARQIADYLVGMNYTELYTLKLQQKGLSGVVSLGRVQTPVNTLVVEN
                     DLSIKNFKSEKYKVIECHTVDREPKVIFKNSREYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            complement(2436..2654)
                     /locus_tag="IV038_002745"
     CDS             complement(2436..2654)
                     /locus_tag="IV038_002745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342897.1"
                     /translation="MELIPYVYQHLYKAKSMKDGNEVVGSLICCPPFSYIATIESMKK
                     MCVDELNDGEVKNLKLTRVLERSIEKFK"
     gene            complement(2671..3030)
                     /locus_tag="IV038_002746"
     CDS             complement(2671..3030)
                     /locus_tag="IV038_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAP8342898.1"
                     /translation="MNIDGIYNESDFKKGDIAVIRSEYDFPSEIGIHTVEGETVCRYW
                     GRKGNLILLLDTDSGEKLKFCFWHNTNYCCEPIPKRLIDVNKESVRYLADGSRIRLTY
                     QKSENSSITRVRDIVLI"
     gene            complement(3166..3465)
                     /locus_tag="IV038_002747"
     CDS             complement(3166..3465)
                     /locus_tag="IV038_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8342899.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            complement(3699..5300)
                     /locus_tag="IV038_002748"
     CDS             complement(3699..5300)
                     /locus_tag="IV038_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8342900.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILR
                     TSRGRYGSTKIATSKFVEAKILNTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
BASE COUNT         1783 a         1081 c          690 g         2132 t
ORIGIN      
        1 aattatacgg actttatcaa aataagcgga aaaacaaatg atttccgcca ctaagaatta
       61 aaattctttc tttataactt tgtttttatt atcaaagaca agataagcac tatatttttt
      121 attgtcttta cttataaaac ccgttacttt ctctttagtt ttcccatttt ctaacaattc
      181 tttgactgtt tttgaactta aagttttccc actattattt ttatagaaaa taaaactttc
      241 acctgttgct aattctttta tttcaaatac ttttgctttt tcaactattt cataaccatt
      301 tactgtatcg tgtttgctct ttttatcgcc ttttggactg ttcttttcaa actccattac
      361 cactttcatc acatttggat cgaaaagtaa tcttgcatta aaagcttttc cttcttttga
      421 cttaaagccg gaaaggactt tatctgtacg attatttagt aatagttctt gaagaacttt
      481 tttattgatt gcttttcctg atatattttt aaatatcacg aactgttcac cactagtaat
      541 atttaaaata gaataaactt ttttcttttc ttccacttga taactaccta tatcaaattt
      601 attttgattt acagtctttt ctaaataatc cgaagtaaat tctacttttt tcaaatctga
      661 aacagtctta ttgatatagt tatagatatt tcttaaaaaa gcttcctgtg tatagctacc
      721 ttgcgcaatt ttcccaagtg attcttccca tatcgctgta ttttctggtt ttccaatcaa
      781 taggtcttta ttctttgtca aatcccataa taaatatccc tttggagtag gataaacatt
      841 cgtgttttct atcttaatat attccctatt tagaagagtg tctacaatat tagaacgtgt
      901 agcgggcgtt cctagcccta atttcgccat aataccggtt ttacctaata atttaccttc
      961 agtcaacctt ttaggttcct tagttaattt atcaataaat ctaatatcta tatttattgt
     1021 ctcttgttca tgaaatttag gtaatgtttc ctctttatca tcagattcat ctaaattagt
     1081 atctacttgc ttccaaccta aagatatagg tacattacca cttgtggtaa attctaagcc
     1141 gtttacatct aatgtaactt ttgtattttc gtacttgtaa ggttctgcaa acattagaca
     1201 cgcacgccta gtaacgatct cataaatatt tttttcatct aatgtcagat ttgctatatc
     1261 aggaatatcc attgttggta ttagtgcata atgctctaat actttgtcat tatctacaaa
     1321 ccgcttttta ggctctaaac aaggtgtatc aatactgatc cctaaaacac tcttatacac
     1381 gtctaaattc gcttttaaat actcaaattc atttgtagtt atcaattcac tatcggttct
     1441 tggataagat aaatatccac gatcatatag actttggata attttatctg tttttttagc
     1501 agaatatttc cattttttat tagcgtattc ttgaatacca cccaagctaa acaatttttg
     1561 tggttctgtt tgctttttac taacctcaat tttttttatg attccagtac tggcggaatc
     1621 aaggccgaat tttttagtgt cttcttcaaa tttttctaat ttaaagtatt ctcgactatt
     1681 tttgaatata acttttggct ctctgtcaac agtatgacat tcaatcactt tatacttctc
     1741 agacttaaaa tttttaattg ataaatcatt ttcaacaacc aatgtattaa caggtgtctg
     1801 aactctacct agtgatacca ccccagataa gcctttttgt tgcaatttta atgtatacaa
     1861 ttctgtataa ttcattccca ctaaataatc tgcaatttgt cgtgcatttg cttcttcaaa
     1921 gaaggaaata gtttcctctg catttctaag gtttttaaat ccctttatta tttcactttt
     1981 aaccaaagaa ttgatccaca accgcttcat aggcttctta aaaatagatt gcttacacag
     2041 cttaaaaata ttataagcaa tattctctcc ttcacgatct ggatcggtgg ctataataat
     2101 ttgatcagca ttttctaagc acgacttgac tacagtaaat tgctgactct ttccctttga
     2161 aacttcatac ttcaactcat ccatttttgg taaaaaagga agattagcta aatcccattt
     2221 ttctttgtta ggtagccatt catatttttc cggcattgat aatccaacta aatgtcctat
     2281 accccaagtt acaatcgtat ctccaaatac ctcactttga aattcaaact tccctccact
     2341 ttttggaatt ttcttatcgg ctaacgcaaa agcgaatttc tctgcttgat ctggtttttc
     2401 tgctaaaatt acagttgcca taaatactct ctccttcact taaatttttc aattgaacgc
     2461 tctaatacac gtgttaattt taaattttta acttcaccat cattcaattc atcaacacac
     2521 atcttcttca tagattcaat ggtagcaata taagaaaatg gtggacaaca aatcaacgaa
     2581 ccaacaactt cattaccatc tttcattgat ttagccttat acaaatgttg atacacataa
     2641 ggtattaatt ccataatcct tcacctccca ctagattaaa acaatatctc ttacacgagt
     2701 aattgaagaa ttttcagact tctgatacgt taatctaatt cttgaaccat cagctaaata
     2761 tcttacactt tccttattta catcaatcaa tctttttgga ataggttcac agcaataatt
     2821 agtattatgc caaaaacaaa actttaattt ttcacctgaa tcagtatcta ataataatat
     2881 taaattccct tttctgcccc aataacgaca tacagtttct ccctctactg tatggattcc
     2941 tatttctgaa ggaaaatcat actctgaacg tattaccgct atatcgcctt tcttaaaatc
     3001 tgattcatta taaatcccat caatattcat taatagcccc cttttttatt tacctgatat
     3061 atattaattc taacaaaaaa acagccaata tcaaactatc aaaaaacaga gtagctatct
     3121 gtctacttgt agtcaaaaaa aacaaattat tcttttcttc tttctctaat cttcaatata
     3181 ttttttattc cgttttgggg ctgaaatttt caaatactct ttatactctt tttcatactc
     3241 acacaagcaa tcataaacct cattcatatt ccctcctgaa gctagagaca aagcatacaa
     3301 aaaagaaata ggcaaatcag ctacctcacg ctctctagta atccaagacg atagagttcc
     3361 ttgtttaaat ccatataact cacaaaacac ctcaacagat aaacctagct taccgataat
     3421 aaatacattg atcgggtgtg agtaaacata tttcttaatt accaaagaaa atcttcctcc
     3481 ttcaaacata actgcatacg cagttataag ctgtattccg tacacgtgga ttaagggaaa
     3541 agacacaagt aaaaatagtt ctatacactc catacataaa agagagtact aactactaat
     3601 cattagtata cgctaaaaca ggaataggag tgggcagaaa cactagatat gttaattggc
     3661 aatttagcta tttaaaaatc aaaaaaataa atagaactct aacctgtatt agctcctcta
     3721 attggcgcat gtttgctctt ttctctttct ttcagtatgt tcgcatggaa cggtactgtt
     3781 ttgtcttcat aacgtcttaa aatcgtttct acgtggctat ttgctgttcc taataattct
     3841 aagacaaata cttgtttatc ttttttattc tgaataactg tatttaatat tttagcttcc
     3901 acaaatttgc ttgttgctat tttagtcgat ccataccgac cacgagacgt tcgtaatatc
     3961 actgtattcg ccgttctaag ctcttttaaa atcaccttta acgtagatac aggaatttgt
     4021 gtttcttcag atatagttcc catagagaca cttaaaaaca actcattttt tttcaatgat
     4081 ctttgttttc tttctaagta agctaataaa tcagctttac tttcttcaaa atgactatat
     4141 tttcgttctt ctctaggttt agcaaatttc acccaattag acggatcgac acgattccct
     4201 ttacgtttaa cagcttgtct atttacattt attgagtatt tttcaaactc ggtaggtgta
     4261 gcataatttt cgataatttc attgacatat gaacttttag cacctttgta atcgccacga
     4321 taagcacttc ttattgttct ctcaacttca gaataggaca aatcattatt gaattgaagt
     4381 tgatccaatt gatccataca ctcgccaata gataagccac ttgatttaca tgccagggct
     4441 aaggtaaaaa tagttttatt tctatctcct tcagcaatat ccgtatgaat taaaaagtcg
     4501 taccatttag tatcaatttg tcgtttagac cctttcttgc cagtagaatt aggagctaat
     4561 aatgacaagt tagacctctt taaagcatat tctttttctt caaagtcttt tgaccatttc
     4621 aaataccctt caaacgtatc tacgaaatta ggttcataat gtacaatatt ttttttagtt
     4681 ggaaatctaa aaataccaaa atgattacag cccatatcga cttgtggtaa acgattttgg
     4741 atagattttc tgatattttc tgatattctt ttagcaactc tcaaagattt aaaatcattt
     4801 gaattagaaa tatagcttgc cttatcaaat gaatcagttt cttggttata gttttgaatc
     4861 agaaaatagc aatgataacc ttttggcgta tctacaatca gtgtaggcaa taacccttct
     4921 tccaatagat aaagaactag taaattcaaa tccgttttct tttctcctac tggatagtca
     4981 atatctgcta caaaagtatt aatctgaatt aagttagctt ctttgtgacc aaaaattgtt
     5041 ttgttttcac cgccaccaag ccatgaatac acgttaggag tccagtgtgt taatacatct
     5101 gaatcttcca agacacgatc aatagaacga tatataagac ctttagtctt tatcatgttt
     5161 gttttagaag taaatccggc aatagcacca atcttattgc tattcttttt aaaagcaact
     5221 agatttttgc tattaaactg tttaattcca tttttaagaa ttttagctaa aacgtccgca
     5281 tactcgcaac tattcaacat tgcactttct ccttgctatc aattttgaat agcaaaaaag
     5341 acatagggta actttagatt ccgtaataag gagtcctaaa attacactat gtctttagag
     5401 taagcactta aatgctaaac tttaaacgtg caaaaattaa aatgagttgg tagctcatct
     5461 tggtggactt cgtggattgc caaatcgctt ttcttactac agaagttcca ttaaataaga
     5521 ttggtagtct tatttaatta tctttttcgc tatttttttc gttacattca tacatagtag
     5581 agagtctgta actaactttt ctactagaat tttttagtgt tcctttggct tcagaactaa
     5641 taccaacatt ataagctaat agaattactt ttaacaagag tttttt
//
LOCUS       DACDCJ010000132         5446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDCJ010000132 DACDCJ010000000
VERSION     DACDCJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.133"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            137..718
                     /locus_tag="IV038_002749"
     CDS             137..718
                     /locus_tag="IV038_002749"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP8342901.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            721..2028
                     /locus_tag="IV038_002750"
     CDS             721..2028
                     /locus_tag="IV038_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP8342902.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2444..2932
                     /gene="purE"
                     /locus_tag="IV038_002751"
     CDS             2444..2932
                     /gene="purE"
                     /locus_tag="IV038_002751"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP8342903.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2916..4049
                     /gene="purK"
                     /locus_tag="IV038_002752"
     CDS             2916..4049
                     /gene="purK"
                     /locus_tag="IV038_002752"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP8342904.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4062..5357
                     /gene="purB"
                     /locus_tag="IV038_002753"
     CDS             4062..5357
                     /gene="purB"
                     /locus_tag="IV038_002753"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP8342905.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT         1689 a          934 c         1280 g         1543 t
ORIGIN      
        1 ataagtaaaa gattggctat ttcacccttc ttttcttatg ataaagtgga gggtgttttt
       61 ctttaacaga aacatagtag gtttttgcac aaaagcggtt atttcggtat taataaatga
      121 taaaggagca aataaagtga aagaattagt tgaacgtatt caaaaagacg gacgtatatt
      181 aggcgagggc gtattaaaag tcgacagctt cgtcactcac caagtagacc ctgtattgat
      241 ggaacaaatg ggaaaacggt ttgcagaagt atttaaagaa caaggaatca cgaaagttgt
      301 gaccattgaa gcttctggga tcgcaccagc tttatttgct gcaagagaat tagatgttcc
      361 aatgatcttc gctagaaaag caaaaagttt gacgatggat gaagaattat tgacagcttc
      421 tgtttattct tttacgaaac aagtgacaag tacgatttct atttctcgta aatttttatc
      481 ttcagaagac aaagtattga ttatcgacga tttcttggca aacggacaag cggcaaaagg
      541 attgatcgaa ttatgccagc aagctggagc gcaagtagaa ggaatcggga tcgtcatcga
      601 aaagtctttc caagatggac gtcagttact tgaagaaatg ggattgagag tcgtttctct
      661 tgcgcgtatt gcttccttga cgaatggaca agttgagttt cttgaggagg acgcataatc
      721 gtggaaaaag aaacacaaaa tggaaaagct gcggttttag ggctgcagca tctgctagcg
      781 atgtatgcag gagcagtagc ggtgcctctt ttgatcggta caggactagg ctttaatcaa
      841 gaacagatga cttatttgat ttcaattgat atctttatgt gtggattagc gacattattg
      901 caattgacag ttaaccgatt tttcggaatt ggcttgcctg tcgttttagg atgtgcgatc
      961 caagcagtag ctccactgat cttgatcgga accaatgaag gagtaggagc tatctacgga
     1021 tctattatcg cttctgggat ctttgttgtg ttagtttctg gtgtcttttc taaaattaaa
     1081 aaactattcc ctcctatagt aacaggaacg gttatcaccg ttattggttt gactttgatt
     1141 cctgtagcaa tcgagaaaat gggtggaggt aatgcaacag cagctgattt tggtgacaaa
     1201 acaaatctgc tacttgcatt tgtgacgatt ctcctaattg taggcgtgca aatgctggca
     1261 aaagggttca ttcgttcaat cgcggtattg attggtttag tcggaggaag catcttagct
     1321 gctttcatgg gaatgattga tctgagcgcg attggagatg cgccgttgtt ccatgttcca
     1381 caaccctttt attttggtaa accaactttc gacgtatggt cgattttact aatgatcatc
     1441 atttccatgg tcagcatggt agaatcaaca ggtgtatatt ttgcacttgg tgacattact
     1501 gggaaaaatg ttggagaaga agaattgaaa aagggatatc gtgcagaagg acttgccgtt
     1561 atcttaggcg gtattttcaa tacattccct tatactggct tttctcaaaa tgtcggattg
     1621 gttcaacttt cgggaatcaa aacaagaaaa cccatctatt tctctgcatt cttcttgatt
     1681 gttttaggat tattccctaa aatcggtgcg ttagcacaaa tcatccctga gccggtactt
     1741 ggtggaggaa tgttagtcat gttcgggatg gtggcagtcc aaggaatccg tatgctgttg
     1801 gaagttgatt tcagcaatga taaaaatcta ttgattgttg ctgtttctgt tggatttggg
     1861 ctgggtttca atgttacgcc gacattattc agtcaaatgc cgcaaacatt gcaaatgttc
     1921 actggaaatg gcatcgtaat gagtagcatt acggctatca tattgaattt gatctttaat
     1981 ggattgaaaa aagatgaaga aatattggaa gaagccttca agaagtaata gagcaaaaga
     2041 aaggttgtga aatcagcgtt ttgacctgat tgttagagaa acaaacggaa aaatagttcc
     2101 tcgtattttg ccgtttgttt gagctaatac cgagggtcag ctgattgaac accgttgaag
     2161 gaagaggtcg tgagcctgcc gttcatctcc gagtcacaag gaacaatttc aaaaaaaagc
     2221 ttctcatgtt tgttgaatat tgttccttgt gaccgaggag ttggcaggag aacaccgttt
     2281 atccataaaa aggaatgtga cctaactttt gtcacattcc ttttttttat ctttaataaa
     2341 cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca tcaaaaaaag
     2401 tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag caaaaatctc
     2461 tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc aaatattgga
     2521 tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc cagatttgat
     2581 gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg caggagcagg
     2641 gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg ttatcggtgt
     2701 accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg tccaaatgcc
     2761 tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta atgctggact
     2821 acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat tggctgaaag
     2881 acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt aaacctttgt
     2941 tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg atggcgatta
     3001 gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat tgtccagccg
     3061 cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg ctgaaagagc
     3121 ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta gaggcgttaa
     3181 atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg attacgcaag
     3241 accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct ccttatgcaa
     3301 cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat ccttgtgttt
     3361 tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa agtcgggcgg
     3421 atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa gcatggatcc
     3481 cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat ttcacgactt
     3541 ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt gctccagccg
     3601 cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt gcagaagcag
     3661 tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc gggctatatg
     3721 tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa gcttgttcta
     3781 tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga gaaaaagtac
     3841 gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg gaaagctatc
     3901 gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa caagcaaaaa
     3961 aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa acgctggaag
     4021 aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa cgttatacac
     4081 gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg ctcgaagtcg
     4141 agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa gatgtgaaaa
     4201 aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa aaattaacac
     4261 gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa gaaagaaaat
     4321 gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc tatctcatca
     4381 agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc attggcgaaa
     4441 aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc catgctgaac
     4501 caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg aacatcgaac
     4561 gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca gtgggaacgt
     4621 tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg atccgtccac
     4681 aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc tcagcgatgg
     4741 cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg caaaaatccg
     4801 aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct gctatgccgc
     4861 acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg gtgcgcggac
     4921 acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt tctcattctt
     4981 cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg ttaaatcgtt
     5041 ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg aatatgtctg
     5101 caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat aagggaatga
     5161 caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg gacaaccaag
     5221 tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta tctaaagaag
     5281 aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg atattcaaac
     5341 gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag aatagcaaaa
     5401 ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttag
//
LOCUS       DACDCJ010000133         5330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDCJ010000133 DACDCJ010000000
VERSION     DACDCJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.134"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(153..551)
                     /locus_tag="IV038_002754"
     CDS             complement(153..551)
                     /locus_tag="IV038_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP8342906.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IV038_002755"
     CDS             complement(548..1321)
                     /locus_tag="IV038_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8342907.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IV038_002756"
     CDS             complement(1318..2001)
                     /locus_tag="IV038_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP8342908.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IV038_002757"
     CDS             complement(2168..3055)
                     /locus_tag="IV038_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP8342909.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IV038_002758"
     CDS             complement(3052..3921)
                     /locus_tag="IV038_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP8342910.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5326)
                     /locus_tag="IV038_002759"
     CDS             complement(4058..5326)
                     /locus_tag="IV038_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP8342911.1"
                     /translation="MAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDETFNIKAV
                     NEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQGYLTSY
                     PDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEAGYTEED
                     MENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGETVTIQD
                     NASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQGAENQS
                     GKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNEELMGTL
                     AKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETYAIEAVV
                     AEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1646 a         1191 c          894 g         1599 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac
//
LOCUS       DACDCJ010000134         5011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDCJ010000134 DACDCJ010000000
VERSION     DACDCJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.135"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..2018
                     /locus_tag="IV038_002760"
                     /pseudo
     CDS             6..2018
                     /locus_tag="IV038_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2286..2696
                     /locus_tag="IV038_002761"
                     /pseudo
     CDS             2286..2696
                     /locus_tag="IV038_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2757..4217
                     /locus_tag="IV038_002762"
     CDS             2757..4217
                     /locus_tag="IV038_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8342912.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGNPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4228..>5011
                     /locus_tag="IV038_002763"
     CDS             4228..>5011
                     /locus_tag="IV038_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8342913.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGAEQGKTFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAEVIAQNGAFDAKLDPNETQATQEAFEDYLKEK"
BASE COUNT         1949 a          826 c         1065 g         1171 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg attgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaatcga aaagatggtt gaacaccaaa
      121 gaaagaccaa cggtcaacca atgaacgata agagaaatgg gcgttcttgg tttaggcaac
      181 aagagcgaat cgaaaataaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggctataac aagtacggcg
      301 gactggatat gacgatcgag aatattccaa gaatcagaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gtctttttca gccgccacga tccgaaaata tcaacggaag ttggaaacat
      421 tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa tatcctccct gaagtacaag
      481 cgatcattga cagcggacga gtaacgcaat ggaaaaaaaa tccgaccatt tattttctga
      541 aaggctatcg aaaagtcgct ttagaactga atgaaaacgg ggaatttgag gaatcaaaaa
      601 aataccaagc cacgtcggaa gaggaaaaag cagctattcg tgaattactt gaagaggctg
      661 cgccaactga ttctctgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga gatcgtaaca ttcgaattga
      781 tcgatcaact taaaacgttg gataaccaat acgctgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tagatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccg atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 agaaggaaca aaaacaaacg gttaaaaatg aacctattac gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac ggcagaagag attaaacaag
     1081 aagttaaatc cctttcgcaa caggcactgg atgcggtgaa aaagcacact cattctcctg
     1141 aagacgtcaa agaacttctg aactttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gccgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaaggaaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgcg gctttgttcg agaaacagag gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaacac agttgaaacc tgaagactat ccgaaaatct
     1501 tcccgaaccg tgtctttgac tttcaattag atactaaagg acagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatattggga tacaaattcg agacatgtca gagagtgaaa
     1621 tctatcaaag agaattagga caagcacgtg gggcgtacgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagcg acaagtattc
     1741 acgaattagc acatgctaga atgcacaaat tttcagaatt agacaccgca accaaagaac
     1801 tgcaggcaga aatgacttct tatattgtat gcaaacattt tggaatggac acgtcggaaa
     1861 aggctattcc ttacattgca gcatggacga agaatggtca gactttggat gataaggaag
     1921 ccgcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctac ggaaatcaat caatacagag aaaaagaata tgaccaagaa aaaaaacgac
     2041 cacaaagcgg taatatagaa ctggataatg caacaaaaga actacaaaaa caagcaggag
     2101 tggttgtctc aacttaccga cattggtttt cagatcatga agaaacacgt ccgattttaa
     2161 taaaagagtt tgaaaaacta ttgacggtct ataaagagaa tcaacaggta ctgacaaaag
     2221 aaaacttagg agagaaaaag gaagctttag caattacaga acaattaaac cattacgaca
     2281 atcattcttc ggaagatgct ttatatgtag taagtgataa tataagtcaa tactatttta
     2341 gaaatagaat ggaagttcct gtatttgaga aacttaaaga tagatcagaa atagagaagg
     2401 agaaaatagt caaaataggt actctatgga ttgataaaaa agatggaaaa gtcatggaag
     2461 aaaatacagg tcgaacatta cacctgaagg aagcggcgtt tattgaagat cctcagcata
     2521 atcaagtgat tttaaaagga aaaagttatg gaaaagagat ccaaacggcg ttatcttcag
     2581 aagagtttgc gctaggaaat ccattccgat tttcacttga aacagtgtca gaagtggcca
     2641 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc gtataaaaca
     2701 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt ttgaaaatga
     2761 ccgagaaaaa aatgagtctc attgatcgat gtaaacagat cgatatagtg gattttgctc
     2821 gaaacaatgg aatggctgta gtcaataaag gacgagacta tcgcttagaa gaccatgatt
     2881 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
     2941 acattattga attagctaaa ctttttttca tagacaaaga gattcaagat cctaagcaac
     3001 aatttaaagc agctactgat tttattctaa agaatgaaga caaaacagaa cgagtggaaa
     3061 atcttcattt tgagacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
     3121 aaaaaggaag gaattatctg aaggaagaac gaaaactgcc cgattggttg attgattatg
     3181 cagaaaaaga aggattgatt gccgaactaa agccaaaaca tgaacggcaa aatttcttag
     3241 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaaag
     3301 agacagtagg ggctagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
     3361 gcacatataa gcatatagat aaaaattcga cagcgaatca tggatttaac ctaaagatcg
     3421 gtaaccctaa acagttaaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
     3481 tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta aaacaccatg
     3541 tgattagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
     3601 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
     3661 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
     3721 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
     3781 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
     3841 cgaaccaact ggtttccgct cacaaagtaa atgcgtcttt tggccagcag ctttctgtta
     3901 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
     3961 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
     4021 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
     4081 atcatgaaca tgaatttgta cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
     4141 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
     4201 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
     4261 caagcgaaag cttatgccga tagcttctct aaagccactc gaaaagacgg gtattttgaa
     4321 atccaagatc gattgtttcc tggtgaaact gtgatcacgt atggttttgg tcatttagtc
     4381 gaattagatt cgcccgatat gtacgatgaa aattggaaac aatggtcatt agaacatttg
     4441 cctattttcc ctacacatta tcactatcat gttcctaaag ataagaaaaa gcaattcaag
     4501 gtggtgaaac agcaacttca atcggccgac accattatta ttgcgacaga tagcgatcgt
     4561 gaaggggaac tgatcgcttg gacgatcatt caacaagcag gcgcagagca gggaaagacc
     4621 tttaaaagac tttggatcaa ctccttagaa aaagaagcta tttatcaagg atttcagcag
     4681 ttaagagacg caggagaaac ctatccgaaa tttgaagaag cacaagcacg ccagatcgct
     4741 gattggttga tcggcatgaa cggcagccca ctgtatagtt tactcttgca gcaaaaaggc
     4801 ataccaggaa gtttttcctt aggcagagtc caaaccccaa cgctttacat gatttatcaa
     4861 ttgcaggaaa agattagaaa tttcaagaaa gaaccttatt ttgaaggaaa agccgaagta
     4921 atcgctcaaa atggcgcatt tgatgcgaag cttgatccga atgaaaccca agcgacgcaa
     4981 gaagcatttg aagattattt aaaagaaaag g
//
LOCUS       DACDCJ010000135         4994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDCJ010000135 DACDCJ010000000
VERSION     DACDCJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.136"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            24..695
                     /locus_tag="IV038_002764"
     CDS             24..695
                     /locus_tag="IV038_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8342914.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            754..1194
                     /locus_tag="IV038_002765"
     CDS             754..1194
                     /locus_tag="IV038_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342915.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1206..1874
                     /locus_tag="IV038_002766"
     CDS             1206..1874
                     /locus_tag="IV038_002766"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP8342916.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1938..2561
                     /locus_tag="IV038_002767"
     CDS             1938..2561
                     /locus_tag="IV038_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP8342917.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2617..3003
                     /locus_tag="IV038_002768"
     CDS             2617..3003
                     /locus_tag="IV038_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP8342918.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3216..4607
                     /locus_tag="IV038_002769"
     CDS             3216..4607
                     /locus_tag="IV038_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP8342919.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1655 a          830 c         1065 g         1444 t
ORIGIN      
        1 atgatgaaaa aagggggaga aaaatgaatt ttttagttgt tttaaaagat actctgattg
       61 agcgatctac atttctatat gttcaattgc aggaaaataa aacattgctg catttttcac
      121 cagagttcca tctagagggg ctaacattag gagaaatcta tcaagcagaa gggctgtcgg
      181 ctgttttgca cttttttaag gtagaattgg aattaccagt aaaagattat atcgagcttt
      241 ctcttgaatc ttgggatcga gcagtggttg aactgtttcc agaaggttta atgatcgata
      301 caaacgatgg gaagatccat ctgactgcag cagaactgca aaaacaaatg cgttatcaaa
      361 tagatgacga aaattctttt tctattttta gacgtcagca aaagattcta aaaagctttt
      421 taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac acagctgttg aaaaaatatc
      481 caggtctgct tcacacgtct attcaatttc ctcaattgat tacgatgatc catcgtttta
      541 tacgtatgca acagctacca tctaagaagc tgtttcttcc attgacggac acatttcgag
      601 tagtcaacag agaagataaa aagaaagtga ttgtgattga ctttaccaga aatcggaatg
      661 tccttcacca agttgcaatg gaaaaggcca attaaggctt tttctttttg gatttttttg
      721 tcataataaa tattagatga tggaggagat aatatgatgc aaacatggtt agaaaggcta
      781 atacgatttc agcagaacta tcacaaactt atgcagggaa gatatgcgaa atttgatcag
      841 ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt tgctaagtcg ttggttgccc
      901 tatagaatcg gacaattgtt gttcatcgtc tttttcggat ggatgatatt tcgtttccta
      961 tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt atttgaaata tcttgataga
     1021 gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag tcaaaatgga tcgaaaaagt
     1081 tatacttttt ttgagtgccc gaattgtcat caaaagcagc gtgcacctaa aggaaaagga
     1141 cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg aaacgaatgt ttaagggaga
     1201 aaagaatgaa attagtatta ttacgtcatg gcgaaagcga agcaaatttt gaaaattact
     1261 ggacaggatg gctagatgtc gccttaacag aaaaaggaaa agaacaagca gcagttgccg
     1321 gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt ttatacctct gttttgaaac
     1381 gtgcgattct taccggacaa gtcgcactag aagaaatggg tcagttatgg cttcctataa
     1441 taaaaacatg gcgtctaaat gaacggcatt atggggatct tgtgggtaaa aacaaagata
     1501 agatgaaaga aatatacgga aaagatcaag taaaaacatg gcgcagagga tatacggaag
     1561 ttccgccgtt aaccgcagaa aatagatttg accgcagata tgatcacttg gatccgcatt
     1621 tgatcccata cggagaaagt ctggaaatga cagtgaatcg gatcattccc ttatggcaag
     1681 atcatctagc accaaaacta aaagatcggc aagatttact agttgtgggt catggtaata
     1741 gcatccgagc actgacaaaa tatttagaag atgttcctga agatcaaatg gatacgatcg
     1801 atatcccaaa tgcacaacca atccagtata cattagcaga tgatttgaca attctggaca
     1861 aaaaaatact ttaaagaaca cctttatcta aaagtgagag atagaaaaaa gaagcaaagg
     1921 aatggaacaa aaggaacatg aaaaattata tatgggactt tgatggtaca ttgtttgata
     1981 cgtatcccgc aatggtagat ggtgcatggc aagcactaaa agattttggt atcagtatgg
     2041 acaaaaaaga aatctacttc aaaatgaaaa agtattctac tagttatttg atcaatgaat
     2101 caaacttgaa tgccagagag tttaatgaat tatttcaccg atatgaaaaa gaaagtacgg
     2161 aagtttcccg tccatttcca gaaacgaaac aagtactgga aatgctaaaa gacaacggcg
     2221 gtcgtcattt catcctgacc caccgtttga cagaatcaac atggggcctg ttaaaagaac
     2281 atcgattggc acatctgatt gaggaagtag tagggatcga tcaggacttt cctcgtaaac
     2341 ctgatccagc atctttaaat tatttgatcg atacttttca tttagaaaga acagacacga
     2401 tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa aaatgcagga gtcgctactt
     2461 gtctttacga catcgatcat tttctgggtg agatcccggc agattatgtg gtaggaaatc
     2521 tgaatgaaat ccttaccttg cagagcatca ttaaaaagta gtatcccctt tcaaaggaga
     2581 tatgttataa ttttcataaa ggagtgataa aattatatgg aaatcagaag acaaaaagaa
     2641 gttgtagaag catttcatta tgatatgaga ttgccgaacc aagaagtaga aacagatttg
     2701 aaagtaggct tctcgccttt aaaagaaacg atcgaaaact atccgaaaga acatagcatc
     2761 gtaggagcac gcttagagtt ccgtttagtg tttgatacat atgttttatc tggaagtgtg
     2821 agtcagatca atcatatcct caatcggaaa attgaaggtc aaaatgatgt cacacaagaa
     2881 gaagtggacg agctagtagc accgttattc gatatcgtac gccgaatgtc ttatgaggtg
     2941 acagagatcg ccttagatca gcctggggta aaattgaatt ttcaatcaga aaaacaagaa
     3001 tagacagtaa aaacatacct gcgatagatt aaagctatca caggtatgtt tttttattac
     3061 taattgtaag caaaaagaat gtcatttcga taaaaatcgt ctaaaatata aaagtcactg
     3121 aaaaaaattt tttcaggagg atggttgaag aatcattctc gggaaccttt acttccttcg
     3181 atggacttgt cattaataat atgaggagga gaaaaatgac aacactcatc acagttttag
     3241 tggttctagt ttttgcggca ttattatatg tgttctatcg tatgcaaaag cgccatacaa
     3301 aattttcaac acgcgtcttt acagcattag gagttggtat cgtctttggc ggtctgcttc
     3361 aattgatctt tggtgtagac gacaaagtcg ttactcaatc aatggaatgg atcgggattg
     3421 taggtcaagg atatgtatca ttcttgcaaa tgctagttat gcctttagtg tttatttcga
     3481 tcattggtgc attcacaaaa atgaaagttt ctgaaaagct agggaaaatt agtgcgactg
     3541 tattgacttc tttacttgga acaacagcta ttgcagcatt gatcggaatt ggttcaacaa
     3601 tgctgttcgg actccaagga gctaaattca cacaaggagc tgcagaaaca gcacgaatct
     3661 ctgaactggc aacacgtcag gaaacagttg aaaacttatc gattccacag cagattttat
     3721 cctttatccc taaaaacgtt tttgctgatt tagcaggtag tcgtgcaaca agtacgattg
     3781 gagtcgttat tttcgcggca tttgtcggaa tcgcttatct aggcgtgaaa agaaaagcgc
     3841 caaaagaagc agcattcttt tcagatttag tcgacagtct gtataaaatc attatgcgga
     3901 tcgtgacgct tgttttgcgt ttgacacctt atggagtatt tgctttgatg acaaacgttt
     3961 tggcaacaag tgattttgca gctatcgtca atttaggtaa atttgtcgtt gcttcttacg
     4021 tagcattgct tgctgttttg attgtccact cactgatttt gattggtgca aaagtcaatc
     4081 caatcaacta tttcaaaaaa gcctttccgg tattgagctt tgcgtttact tcacgttcaa
     4141 gtgctggagc tttgccattg aatatcgaga ctcagacaaa agctttagga gtagatgatg
     4201 caacagctaa tttctcagca agttttggtt tgtctattgg acaaaacggc tgtgctggcg
     4261 tttatccagc tatgttggca acgatcgtcg caccagcagc tggagtgaat gtctttagtt
     4321 tggaatttat tttgatgctt gtggcaattg taacaatcag ttcttttggt gtagctggag
     4381 tcggtggggg cgcaacattt gcttcactga tcgttttagg ggcaatgaac ttgccagtag
     4441 caatcgttgg gttagttatc tctgttgaac cattaatcga tatggcacgt acagcagtaa
     4501 acgtcagcgg tagcatggta gctggtatcg tgacaagtgc acgcatcaaa gatttagatc
     4561 gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa tgcatagtca aaaagaggtt
     4621 gtgatagaag tggtcagctt taagaaataa gaagaagttt cctaaaaata gtttctcaaa
     4681 tttttgtgaa atttcggctt atttccgaga agctacttct gaaacaccgt gaataagagg
     4741 ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4801 attttacgtt tggttgagct tattaccgca gatgcaagct tttgaacacc gtttaagaaa
     4861 gaggtcgtga gcctgccgtt tcactctgag tcacaaagag caatttcaaa aaatagcttc
     4921 tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg tcaggagaac accgtttacc
     4981 agaggttgtg actt
//
LOCUS       DACDCJ010000136         4919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDCJ010000136 DACDCJ010000000
VERSION     DACDCJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.137"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IV038_002770"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IV038_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP8342920.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="IV038_002771"
     CDS             complement(2064..3062)
                     /locus_tag="IV038_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP8342921.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="IV038_002772"
     CDS             complement(3288..3941)
                     /locus_tag="IV038_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP8342922.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IV038_002773"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IV038_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP8342923.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1458 a         1023 c          756 g         1682 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa catatgaaac agcgtataa
//
LOCUS       DACDCJ010000137         4854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDCJ010000137 DACDCJ010000000
VERSION     DACDCJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.138"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(143..1183)
                     /locus_tag="IV038_002774"
     CDS             complement(143..1183)
                     /locus_tag="IV038_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP8342924.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1532..1858)
                     /locus_tag="IV038_002775"
     CDS             complement(1532..1858)
                     /locus_tag="IV038_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342925.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1845..2633)
                     /locus_tag="IV038_002776"
     CDS             complement(1845..2633)
                     /locus_tag="IV038_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8342926.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3001..3291)
                     /locus_tag="IV038_002777"
     CDS             complement(3001..3291)
                     /locus_tag="IV038_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8342927.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3427..3606)
                     /locus_tag="IV038_002778"
     CDS             complement(3427..3606)
                     /locus_tag="IV038_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342928.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(3603..3953)
                     /locus_tag="IV038_002779"
     CDS             complement(3603..3953)
                     /locus_tag="IV038_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342929.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3946..>4854)
                     /locus_tag="IV038_002780"
     CDS             complement(3946..>4854)
                     /locus_tag="IV038_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP8342930.1"
                     /translation="RRKKLAQMIQENIKEELGLIATVGVGDNPLLAKLALDNEAKHNE
                     GFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRL
                     GVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQV
                     AIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEG
                     QEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSM
                     LEGARSITRSTLVGGHAGGNGGIKND"
BASE COUNT         1408 a          880 c          697 g         1869 t
ORIGIN      
        1 cgtttgtgat tgttttcata aacttttaat ctaaaaactc cggaataata gctatttatt
       61 tgtaacattt gtaataaaat taaaatcata aaaaagttct aatttaaaat tgatcatttt
      121 tatagtgatc gctttttatc aactactcta gccaattttc cgtactaact gttggtaatt
      181 cgttaaatgt ttctctttgt tcgtttgcaa gctttgccgc aatagctttt tcgacaaacc
      241 aattttttac ataaacgaaa attagattct gcaaattgtt tactttttca gttttttgtt
      301 tttgataagc tttcagcaaa gtcatatata atcctttgtc agcatcaatt ccataagatt
      361 ccagttcttc ataaacaaca actgtttgct ctaattcttc agcttttttc tttgcattat
      421 gaatagtttg gacggttttt tgagcgtctt cgtaagtact agaaaatgtt tttatcaagt
      481 ttaatacatt ttctggtaca aatgtattta tactgtcctc tttcatgatg tcgacgaagt
      541 tatctagtaa aacttgattt tgtaactgat cagtttcagt gtctattatg tgtctattag
      601 tgtctataga attagaatag aatagattgt ctgcgatttt cgcaattacg gttgagtcct
      661 tgatatgact ggattttgcc gtatttgcga ttttcgcaat tacggtggta tcattgattt
      721 ctcgcggttt tgccgtattt gcgattttcg caattacggt ggtatcagta ttgtcttcct
      781 cttccatgat tttaaagacg tcttgattcg ttacttctgg cttgcctaag tacaataaat
      841 taggcatgga ttcaatctta ccgtcacgtt tatttactct tcctctcttc tgtttcagaa
      901 gacctgcttt ttctaattcc tttttgattt tagatacttt accttcgcgg cattgtaaca
      961 atttcattaa ttcttcaact gtgaatatga aaaatatatt atcatcagaa tctacccagt
     1021 tatttttgac tgaataatca aatcgatctt taagtagcat ataagcaatt tttgcatcat
     1081 ttgatagatc catgtatttt tcgttagtaa aaaatacctt aggcatttga taatattttt
     1141 cacgataaat ttcttttgat ttgtaataat taaaatcatt catagccaat cctctttctg
     1201 taggatagcg ttttgttcaa aactatgtta caatgaatta aacaaatata tctatccact
     1261 atatattttt ctactgctga taagtcgcca aactttgtta aagcagtagt ctttttttat
     1321 ctttttttag tttaccttaa aaaataaatt aaagcaaacc gctctatttc aaggtttaag
     1381 ctcatagtaa ctatatcacg atattacaat aatattatat cacgatatcg taatattaca
     1441 atatgaagat aagcgactta tcagctttga tactactgat ttttataaaa aaaatatcac
     1501 gatattatga tatcgtgata ttgaatgtaa attaaatttt attttgtttc tttgctagtt
     1561 tatatccttc aatcaagtat tcttcaggta tggactttaa tagcgcatct tctataacgt
     1621 ctggtttact tttgtctgat tcagcaatag tcgtccaata tttgagtgct gacattagaa
     1681 ttggatttat acgaatgcta tctgctgttc ttacactact tccttgatct tttatgactt
     1741 tacttatatt tttcttggtt aattcgattt cttctacttc ttttgattta aaattctttt
     1801 tatttttatt tcctagtaat ctacttactg cagcttgact gcgattatct tctgccatca
     1861 aaatcaccaa cttcttttaa atattcttct gtaaatgctt tatagtcatc ttcagctgtt
     1921 gagggtttat taaaattgat tagatctgat ccagcagctt gcgcttcgtc aacggttatt
     1981 gtcctacgaa tataagtttt gaaaattttt gcaccaatga ctctagaaat ttcggttgct
     2041 gcagacgtca tttctttaga aatgttataa cgcttattat acttagtaaa tagaactcct
     2101 cgtacttcaa gcttgtcgtt cgtatattct tttaccgttt caatagtttc acttaacttt
     2161 attagtcctt ttaatgagaa atacgcagct tcaactggaa tgataatttc atcagaaaca
     2221 gtaaatgcgt tgactgtaag cattcctaaa cttggtggac aatcaattat aatataatca
     2281 tagttgtcct tgattggttg aagtactctt ttaagcctgt gttcacttcc tacttgagta
     2341 aattccctct ctgcagaaga aagggctatg tcggctggca aaacatcaat tcctttatag
     2401 tccagaatag catctgtaat gtcagcctcc ccttttaata cttcataaat tgtaggttgg
     2461 tttgttccat ctatatcgaa acctacatta tctgtagcat ttccctgagg atcaatgtcc
     2521 actaaaagta catttttacc gtaaacaaat ttcaaacatg ctgctaaatt aacgctagtc
     2581 gtagttttag aaactccacc tttttgattg gctactgaca atattcttgc cattaaaata
     2641 tcccccattc atcttatatt ataatataat tatatcataa tattgtgata ttgcaatatc
     2701 gtatatgcat tatattgtaa tattgtgtga ttattttgtt ttaaagatat atatcggttc
     2761 ggatataaag attattcata tgttattcga aattaaaata atcacttttt attactttaa
     2821 aattttttat taatgataag aaatatgttt tgcgagcagc aactagaata atttaaacat
     2881 tactaaactg tctatattgt tactaataat tcccttctta tagtgcattt tcttagaaaa
     2941 aaacaatatt acgatatcat gatattaaga tatcgtaata ttatacaatt gtgattgata
     3001 ttatatatgt ttttttattt ttttatcatg tttaatcttg aaggaatcat attctttttc
     3061 taaagacagg agcttttcat aaacatcaga catgtttaaa ctagaggcca ggcttaaatc
     3121 ataaacgaat gaagcaggta atgattttat tctacgttgt cgggttatcc agcttgcaac
     3181 cgttccttgt gggtaggcat ataactcaca aaattgttct acagtgattc ctaaattctt
     3241 gataatgtaa acattgatcg ggtgcgagta aacaaatgta ttgatagaca tggtgcgtaa
     3301 gcctactttc ttttattatt gcgtttgcaa taatagttta acacacgcat tattctagta
     3361 tctactccta tttgtaaaaa caaaaaagga ttcatagaga taactctata gaatccgtaa
     3421 aataccttat atatataacg ggaagatata aaacctaaaa ttatgataac ctgctgcagc
     3481 ttctacatag aaagagaagc gctcaacacc aacctcattt atcaaacgtt ggccataacc
     3541 ccaaatctct tctgatagct gatcaaaggt tatatacttt agctcatatt gctggataat
     3601 gttcattcta atcgactcca tttattatcc tggtaaaatt cgatatgacg tatttcgtcg
     3661 tagtgaattt tgtcttttcc aacgtagatc cccagctcat cactacctaa gatcttgcca
     3721 atgatatctt ctggatagta tccttcagcg tttcgttctt ctttttgaat agatactgat
     3781 ctatttttta tgactgcagc gttcaatact tccgcaatct cttcagtcgt catttgttct
     3841 tttgctaaat tttgactggt tcgttgttca ttgtcggcat caatttcttc agtatgatca
     3901 gataaataaa accctgccca ctttaaacgt ccacgatcat tgtaatcaat catttttaat
     3961 tcccccattt ccgcccgcat gaccaccaac aagtgtgctt ctagtaatgc ttctagcgcc
     4021 ttctaacatt gaagaagcat gtacaatcga cgtaaaacca tatttttgcc gaatcttgtc
     4081 aataatttta tctaaatttt cttcgtcaat ttgcttttgt ggatcactaa ataaatccag
     4141 ctgaagcgaa tcggtgtaca caagcttgct ataggttatt ccaacatgcc ttacttcttg
     4201 tccttcataa tacttatcaa acaagaaaag acaataattc tgcaattcct ttgtattatc
     4261 tgtgataggg atcttcatct ggtgtgaaaa ccctggtctc gtttctaaga tcgacgtgcc
     4321 aatgtgtaaa tggacacaac ctgttttaca gttatgctgc ctaatgcgta tcgctacttg
     4381 ttccgccatt tcttttacca ccagctcgat ttcatttctt ctagtataat ctcgtggtaa
     4441 cacttgtgaa tttccatatg atttttcctt tgtcggttct ggtgggattg ctatatctgt
     4501 tcgatcgatc ccattagcat ggaagtacag ctgtaagcca atcacaccta gtctattctt
     4561 aatcgtataa ggattccagt tagcaagatc tctaatgctc ataatcccca tctgattgag
     4621 tctttttttc attcgagagc caattcccca aaagtccgtc atttcaggaa tattccatac
     4681 tttttcagga acgttttcgt acgtccattc cgcaacaaat ccttcattat gctttgcttc
     4741 gttgtctaag gctagtttag ccaaaagggg attatctccg acacctactg tagcaatcaa
     4801 tcctagctct tctttaatat tctcctggat catttgagcc agcttctttc tccg
//
LOCUS       DACDCJ010000138         4792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDCJ010000138 DACDCJ010000000
VERSION     DACDCJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.140"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(76..1074)
                     /locus_tag="IV038_002781"
     CDS             complement(76..1074)
                     /locus_tag="IV038_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8342931.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1149..1445)
                     /locus_tag="IV038_002782"
     CDS             complement(1149..1445)
                     /locus_tag="IV038_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8342932.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1438..1731)
                     /locus_tag="IV038_002783"
     CDS             complement(1438..1731)
                     /locus_tag="IV038_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342933.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1877..2428)
                     /locus_tag="IV038_002784"
     CDS             complement(1877..2428)
                     /locus_tag="IV038_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP8342934.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2602..2901)
                     /locus_tag="IV038_002785"
     CDS             complement(2602..2901)
                     /locus_tag="IV038_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP8342935.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2928..3194)
                     /locus_tag="IV038_002786"
     CDS             complement(2928..3194)
                     /locus_tag="IV038_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP8342936.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3481..4224
                     /locus_tag="IV038_002787"
     CDS             3481..4224
                     /locus_tag="IV038_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342937.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(4261..4758)
                     /locus_tag="IV038_002788"
     CDS             complement(4261..4758)
                     /locus_tag="IV038_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP8342938.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1429 a          912 c          696 g         1755 t
ORIGIN      
        1 ataaaaaaac tgattctaag aaaattaaat aatttttctc aaaatcagtt tttctttgat
       61 aaataatatt ttcaattatt taatcatttg aaaattgtta gtcattccaa tacgttgaga
      121 aattcttcca tctgtataaa cacgaaattg ttttccacca gccatggtcg tgtttccatc
      181 atcatccgtt acaatattaa ttgaatagta gcttccattt tcatcttgca ttagatcttt
      241 catatattca tttgtgctaa ttaaattagt atttgataca tttttatcag gagaatcata
      301 caaccataaa gctttattat ttagcacaga ttctttagca gcttcccaac ttgaaattcc
      361 tgaactactt gtagattgtg tcgttgtagt tgttgcttgt tgagtagatt cttctactac
      421 tggtgtttga ggagtttgtc cattcacaat atcactgaaa ccttgtttta actgttgatt
      481 ttgagtttcg ttaaagtaca gataattatt ttcattacct tgattttgtt gcgttaaata
      541 aactttaggt tgtccatttt gaaaaccgaa aaagtaaaca tgctgcccta aatattctcc
      601 agtttctaca tcagaataaa ctgctactaa attgaaatct gcatgaccag ttccatcttc
      661 tgaccattgt acggtaactg gtgcttcatt gattgccatt ttccaatcgc cattaatcac
      721 ggcttgtggt aaaggagtgc catacagact tacattattc tgaagattgt agcttttata
      781 ttcttgacct aatgtctttc cccattgtgt gacaaaagtt tctaatttgg aagctttatt
      841 cgtatcccac aaagtattct taacctcttc agaagaagat tcagtgattg aacttgcact
      901 tgtttccaca gattgcttca tttctgttgt agtttgttca gttgattgcg ttttactttt
      961 tttagtctca attgttgtat cagtttctat agtcgcttta gtcctcgtat tctcagatgt
     1021 acaaccagtg aataaaattc ccattcctat tagtaataac agccattttt tcattatatt
     1081 tccccctttc aatttctccc tcacgtactt attctatcag acaattaatt gatctaaatg
     1141 gtaatctttt aatcgattct ttcccaataa tgtcgatagc ccgtatggca gcggtctctt
     1201 ccttgaggac tctcagatgg attaaaacta gtagaaattt cccttccaca tcctctacag
     1261 cgataagttc tcattatttt tgggctatta gtattatttt tcttagttag agctatgagt
     1321 ccaactatga ttaccccaac tgtcttaatt acttttcctt tatctatatt cggatgcgca
     1381 agcatatatt tatataacca atatccaccg taaattaaac cacctaatac aatcagctta
     1441 gccatgtact cgtctccttt ctttttgttt aattactttt tgaatccatc ttgcagagaa
     1501 accataaact tctgtcaatt gacggatatc tacagttttt tcctgttcta tcatgttttc
     1561 gattcttttt ctaactaact cacgatcgta cattttcatt ggaaaagaaa tctgttgccc
     1621 tcgaaaagca agataaaatt gatagagcaa ttctgaatcc agttccattt cctcaaaaaa
     1681 gtcattcata cttttataag agttactcaa acaagtttca tcaatctcca tctacacacc
     1741 ccctgttatt ttttacataa tgaaaatagc atattactgg gtacattgat aattcatata
     1801 acatgtatca tcacatgtat ttttaaagtc aaaaagcatg taaaactact tataagtaac
     1861 atgagaaaat aaaatgttat ttttcttgaa ataataattg actatctgcc aattgccatt
     1921 cattacgaac atgaatactt agtgggtggg ataacttacc ttctgcattt atcaatactt
     1981 tggtcatctt cttttgtttc gcactcaatt gatcgtaaat agtctttgct tgttcataag
     2041 ccacttgact cgtcgctgta atcgaaccac aagcaaaaat aatggtattg attttctttt
     2101 ctgtaaaaac agtcgttaat atttccaaag attgttcatc atttcctcga agaaataccc
     2161 gaggactttt cgcttttgtg tacgaaaaaa gattcacagc aataaaacca gtaaatccta
     2221 attgtcggat attcttttcg attaataaca tactcaaatc attcgtaaat ggttctgtat
     2281 ctgttggacg aatagtaatg actactgcta atttatcttc cgcttctttt cctttgatcg
     2341 cccattgtcg tttgaaaaca tagcgatggg tatgatgagg atctcgccaa acttctgttt
     2401 gtgtggtcac taattctttt tcaatcatct ctttttctcc tatgctattt tttgttcaag
     2461 cacttcaatt accattgccg ttataagtgt caatactagt tgaaaatatt tcatattctt
     2521 tctccttttt tcttcagaat ttgatgaatc gcggtaagga tatcttctaa acatttcgca
     2581 atcattctca aataatcgat cttattcatc cgttcctcca atctgactcc aaaccaatcg
     2641 tccttctacc agtaacccac gaacaaattc tcgctggctg gtaatatctg taatgaaata
     2701 aactacatag tcttttccat cttctggtgc gcaataaata taaagatgtg tcatcactaa
     2761 acgtggtaga tgaacatctt caggaagcat acccatttca atttcctctt tttccatcgt
     2821 aacggattca tagccccact cttcagaagc agtgaccagc tgtgcacact tttccagtac
     2881 ttcgatagcc gtttctaaca agtcctttcg ttcctttctt ttagatacta gtattcttct
     2941 ggtaataaca tcgtttgata ctcacccgaa tcaatggccc atattttttg agacactgat
     3001 ttcaaacgaa gcttaggtga aatcagatac tcttttcttt cttccggttc ttcttgacga
     3061 tgaaccaact tttgatttcc tttttccgta gaaaattcaa aaatttgaag atagtctaaa
     3121 ggaaattgct tttgaacgtt ttgatcgatt agattccaac ataaagcttg taattccacc
     3181 ggcaaactgt ccattactcc ctttgtgaca taacgttatc ctttattttg aaacatttct
     3241 tttccctcct ttgccataaa aatagcccaa tcttctatta aagaagattg ggcttcatgg
     3301 atataatata tatttgtaaa taaagttaat tcagttataa ttatcaaaac tacacaatta
     3361 ttaaagtttt tcatatctct ttgtttagta agtttttcta aatcttaacc cgtggtgcta
     3421 gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat
     3481 atagtagtct caaaactccc aagaaatgag gactatttaa caaactacga tggttacgtc
     3541 gtgacaactg aacattttga cttagccaat cctgttacta aagtcgatga tatccccgat
     3601 tatgaaatgt acagtcaaac aatcgatagc ttaaataagc gttttggcaa tcgtgtttta
     3661 aaaggcatca aaatcggtta catcgcttct gaaaaggatc ggatcatcga ctatttagcc
     3721 gataaagatt acgatttaaa attactcagt gtgcatcata atgggcaatt tgattattta
     3781 gatgacgaag tcaaagatat ggatcctgct attgtgattc ctcagtattt tgcacaactt
     3841 tcagaagcac tagttgttat agaagcagat gtctttgctc attttgacta tggttttcgg
     3901 gtatttggtc tatccgttgc tgaatttaaa caatatgaag cgcagttttt accgattcta
     3961 gatcaagtaa tcaaaaataa attggcattc gaattaaatg ctaagagcgc ttacttgtat
     4021 gacaatttag cgttatatga atatgtcatc gatttatatc tttcacgtgg tggtaccttg
     4081 tttagtgttg gatcagatgg gcattacctt gaacatttcc gtttccattt cgatgatctt
     4141 tttgctctgt taaaagcaaa tggtgtgacg gaattagcga tttatcaaaa aggaaagcga
     4201 attatggttc cattaccagt ctaaaaaaac gtcctcagca tcaaaagtga ggacgttttt
     4261 ttagatttct ttttctaaaa tcggacaaat acgatcttgt tttcctaaag gaaaatgttt
     4321 gatcccgatt tttttaaatc catgacgttc ataaaaacga atacctgcat gatttttttc
     4381 ataaacagtc aaacgtaaat aacgtttttg tttcgtttta gcataacgat tcgcttcatc
     4441 gagtaaccgt tgccctaacc ctttgttttg ataatctttg agcagataga gccgttgaat
     4501 ctctatagca tctttttcat cattgacttt catataacca atgagtattt gatgatcctt
     4561 aataccaata aatttactgg ttgactgtgc caattctact gccaatattt cagttgtcaa
     4621 ttgtgtctgt aaatatcctt gcatatcaag tggatctgcg ccatcgataa acgattcata
     4681 aaaactgaca acacttacaa cttgtaaagc atctagatca tcataatcta aatacgctag
     4741 tgtgatttga ttgttcatct aactcctcct tttgacatag tatactataa at
//
LOCUS       DACDCJ010000139         4760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDCJ010000139 DACDCJ010000000
VERSION     DACDCJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.141"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            71..1189
                     /locus_tag="IV038_002789"
     CDS             71..1189
                     /locus_tag="IV038_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP8342939.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1317..2693
                     /locus_tag="IV038_002790"
     CDS             1317..2693
                     /locus_tag="IV038_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP8342940.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3094..4674
                     /locus_tag="IV038_002791"
     CDS             3094..4674
                     /locus_tag="IV038_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP8342941.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT         1561 a          785 c          990 g         1424 t
ORIGIN      
        1 aataaaaaaa gaataaaaga atttttaacg aatataatat atactagtaa gaaagaaagg
       61 aggaaatcta ttgtttgaaa aacttagaca gtctaaacta atgttttggt cgcttgaatt
      121 attgatcata gctgcattga tcatgatttc atcagaaata aactttattt tcgaaccgat
      181 cgggacattt ttttccacgt tatttgcacc tgtactgatt gcaggctttc tttactattt
      241 attgaatcct attgtcaagc tgttgatgaa agcaaaaatc aaaagaatat gggctgtcgc
      301 aattgtgctg ctccttttag ttgcagcaat tatttggatt ttacttagtg tcatcccaag
      361 tctagtacag caaatttcct cattggcatc taatatgcct gatttcatca aacaggtaga
      421 aagctggtta aaagaggtag ccgaattgcc tttgttcaag gaagtagata tcaataaata
      481 ttttgagcaa ttagacattt cttacgggac aatcattcag caattcctta gtggagtatc
      541 caatagttta ggttcgatcg ttggaacgat cgcttcggca accgtagtaa tcattacagc
      601 accattcatc ttgttctata tgttgaaaga cggagaaaaa ctagtgccaa acatccaaca
      661 gtttttccca gaaaaaagaa gaaaccaaat tgtggagtta ttggggcaac tgaataaaac
      721 attggctaat tacattagcg gacaagcaat cgaatgttta ttcgttggta ccttcacatt
      781 tttaggatat atggtaattg gtgtggatta tgctttctta tttggggtca ttgcgggatt
      841 gacaaaccta attccatatt taggacctta tctaggacta gcacctgcat ttctagtaac
      901 agtcttcaat gatccggtaa aagcattact ttgttgtgtg gttgtgctaa tcgtccaaca
      961 gttggatggg aatatcattt atcctaatgt aattgggaaa tcactgaaaa tccatccatt
     1021 aacgatcatt atcgttcttc tagtagctgg aaatattgcc ggattgcttg gtatcttctt
     1081 aggtgttcct ttttacgcaa tctgcaagac gatcatttcc tatataatca aaatcgtcaa
     1141 agaagacaag caaaatgaaa acaagaagaa aattgctacg acctcgtagg tagttttagt
     1201 acacttgttt ttattgcaaa gaaaattgct ttgtgatacc attttaacgt ggctatagtg
     1261 ccacgttttt ttctatcgta aactaaaaat taattttata aggagagaat gcgattatga
     1321 atgctgaccc tgagagtcag tcgctgttag cgcagatttt attattgatc gttttgacat
     1381 tgatcaatgc ttttttagca gcttccgaaa ttgctgttgt ttcgatcaat aagaatcgaa
     1441 tcgagcaaaa agctgatgaa ggagatgtga aatctcaaaa gcttttaaaa atattgcaga
     1501 atccaaataa tttcctatcg acaatccaag taggaatcac tttggtcaac attttgtctg
     1561 gtgcttcact agctaatact ttatcggaac ggttagctcc ggtattaggt ggcggcacag
     1621 cggcaaaaaa tattgcaaat atcattgtgc tggctatttt gacctatgta tcgattgttt
     1681 ttggagaatt gtaccctaaa cgtattgctt taaataaatc agaagaagtc gcaaagttca
     1741 cttcagggct gatacgcttg attggtgtag tagctaagcc cttcgtctgg ctgctttctg
     1801 cttccacaag tttattggcg cgtatcacgc cgatgacttt tgatgatgaa gattcaaaaa
     1861 tgaccagaga tgaaatgcgt tatatgcttg aaaacgaagg cgtattgaat aatgaagaat
     1921 tagaaatgct ccaaggggtt ttttctcttg atacgaaagt tgcccgagaa gtcatggtcc
     1981 cacgaacgga tgcatttatg attgatatca acgacactgt ggaagaaaat gtgaatgaag
     2041 ttttatccga aaactattca agaatccctg tttataacga agacaaagat aaagtagtcg
     2101 gaatccttca tacaaagaat ttactaaaag ctgcccataa attcggcttt gataatttag
     2161 atatcaagaa aatcatgcag gagcctttat tcgttcctga aacggtcttt attgatgatc
     2221 tgttatatga aatgaaaaag acgcagaatc agatggctat tttattagat gaatatggcg
     2281 gcgtcgttgg gttagcaaca ctagaagacc ttttagaaga aatcgtcgga gaaatcgatg
     2341 atgaatcaga tgaagttgag aatctttatg aaaaaatcga tgaacacgaa tatatcatcc
     2401 aaggacgtat gctgattgat gagttcaatg aagcttttga cagtgatctg catatgagtg
     2461 atgtagatac gatggctggt tatttgatta cagcattagg catgattccg gatgaaggcg
     2521 aaaagttatc gtttgatgtc gataatatta cgctggtttc tgaagaaatg gaaggttctc
     2581 gaatccttaa aatccgtgtc atttttcatg atccggaaga aacagaagcg gaacctgaag
     2641 aagaacgaag atacttcaga aaagaattcg aagatgacga gccaagaaga taattggaaa
     2701 cttggttatg aaagacgtgt tgcttgaata aacaagcaac acgtcttttc ttttagtcag
     2761 ctgacaaaaa aatatttgac tcttatggtt tggtggcttt ttcattcgct gaatccatgc
     2821 tatactgatt cagttgtagt agttgacaaa ggttgtgaaa aagctatcct gcattaagta
     2881 ataagagcag ccaagcaaaa acagcttttc gtatttttgg cttgggtggg cttattgctg
     2941 ataggctgtc tctagaacgc cgtttactcg gaaaaagaga ctgctacacg gcttttgtca
     3001 cagccctata tgaaattgtc tttctttaag agatcggaca atctgtgcct agagaaaata
     3061 taaataattg aatggaagaa atggagttag gttatgaata atccaaaatt aaagcaacaa
     3121 gtggacaatc gcagaacttt tgcgatcatt tcccatccgg atgctgggaa aacaacgatt
     3181 acagaacaat tactattgtt tggtggggct atccgtcaag ccgggaccgt aaaaggcaaa
     3241 aaaacaggta attttgccaa atcagactgg atggaaatcg agaaacaacg tgggatttct
     3301 gttactagtt ctgttatgca gtttgattat caagggaaac gagtaaatat cttggataca
     3361 ccaggacatg aagatttttc tgaagatact tatcgtactt tgatggccgt ggatagtgcg
     3421 gtcatggtaa tcgatagtgc aaaagggatc gaagcacaga caaagaaatt attccaagtt
     3481 gtaaaaaaac gtggcatccc gatatttact tttatcaaca aactagatcg tgatggtcgt
     3541 gaaccattgg aattgttaga agaattagaa gagctattag atatcgaatc ttatccgatg
     3601 aattggccaa tcggtatggg taaagggcta gaaggtcttt atgatatcta ccataatcgt
     3661 gtagagtttt atcgtccgga aaattatcag gatgaacgat tagtagaatt ggatgaagat
     3721 ggactgcttc ccgaaaatca tcctctaact gaaaattcct tatatgaaca agtcttagaa
     3781 gaagtagaac taatcaaaga ggctggagat gcatttaatc aagaaaaaat tgctcgtggc
     3841 gatcagacac cagtattttt tggttctgca ttgactaact tcggtgtaca gactttttta
     3901 gagactttcg ttgaattggc accagctcct tatggacaca aaacacaaga tgatcagatg
     3961 gtaagtcctt atgaggaaga attttcaggt ttcgtattca agatccaagc aaatatgaat
     4021 ccagcccatc gtgaccgaat cgcttttgtc cgaatctgtt ctggaacatt cgaacgagga
     4081 atggatgtct ggttggaacg tacaaataaa aaactgaaat taagcaatgt cacacaattt
     4141 atggcagatt cgagagaaaa cgtcgaaaaa gcagtagcag gcgatatcat cggtgtatat
     4201 gatacaggca attatcagat tggggataca ttgttcgaag gaaaactaaa agttgcttat
     4261 gaagaactcc cttcatttac tccagagctg tttatgaaag tgactgcaaa aaatgtcatg
     4321 aaacaaaaat ccttccacaa aggaatctat caattagttc aagaaggagc gattcagctt
     4381 tataaaacat atctaacgga agaatatatc attggagctg ttggtcagct acaattcgaa
     4441 gtattccaat atcgtatgtt aaatgagtat aacgccgaag taatcatgtc tccaatggga
     4501 cacaaaatcg ctcgctggat cgatccagaa gatttggatg agaagatgag ttcaagtcgc
     4561 aatatcttgg ctcgtgaccg tttcgaccaa ccgttgttct tattcgagaa ccaatttgca
     4621 gaacgctggt ttgcggataa atatcccaat gtaaaattaa aaagtttgat gtaaagaagt
     4681 cttgacacaa gctgccctgc atcaagtaat aagaatagcc aaacaaaaac agcctctcat
     4741 attttgttta gagccgtgac
//
LOCUS       DACDCJ010000140         4719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDCJ010000140 DACDCJ010000000
VERSION     DACDCJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.142"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..1533)
                     /locus_tag="IV038_002792"
     CDS             complement(<1..1533)
                     /locus_tag="IV038_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8342942.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQ"
     gene            complement(1584..2405)
                     /locus_tag="IV038_002793"
     CDS             complement(1584..2405)
                     /locus_tag="IV038_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP8342943.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(2402..3211)
                     /locus_tag="IV038_002794"
     CDS             complement(2402..3211)
                     /locus_tag="IV038_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP8342944.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(3232..3726)
                     /locus_tag="IV038_002795"
     CDS             complement(3232..3726)
                     /locus_tag="IV038_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP8342945.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(3738..4226)
                     /locus_tag="IV038_002796"
     CDS             complement(3738..4226)
                     /locus_tag="IV038_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP8342946.1"
                     /translation="MFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(4303..>4719)
                     /locus_tag="IV038_002797"
     CDS             complement(4303..>4719)
                     /locus_tag="IV038_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP8342947.1"
                     /translation="KHILSSEMKPVEFNRLIQELIKFFSLEGIADRLNFLNPTIVMSE
                     VETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKAEEPNYDMKPDEDEFY
                     YVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         1452 a         1082 c          759 g         1426 t
ORIGIN      
        1 ttgataaaaa ggcatcaacg tagtgtctga ttcttctcca tcatgcatca ataaattaat
       61 ttttccatcc acaatcacat cacttaaaaa gattaaccct tgctcaagaa tcggtttttg
      121 atagtgcttc actgaatagg ttgttttctc ctcatcaccg attccctcca aaaattgctc
      181 accaaaagga ccatcgatag tcatttgttt acaaattgat tcctttagtt tacaggcaat
      241 gtttttgtat cgcttgctaa gactcagttg ttctagggct tcaaaaattt ctgcaagtcc
      301 aatacaggcc ttccaaagac aaagattgct gccggtatga tactttccaa gtgcaaaggc
      361 gtctgataac caaacagatg gaaaaagcat tggttcccct gaccgacgtt gagctaatat
      421 gtcctcaata attcttgaag cattcagtag aacctgcgga caccgctcaa agaattttgt
      481 atccccagta tgttcaaaat aaagactagc cagtaacgca ctggataacg aattactcag
      541 agaatgattg atacctcctg taaattttga accaggaaac ttaacgccat attgatcaaa
      601 ccatagaacc gttttttgac ataactccgg ttcaaacatg gtgaatggca atgatgcgta
      661 atacatatcc ttattccaaa ttcgattagt agcaggatag ctgccccaat tgctgccgac
      721 gacctgtccc tgtcgattca taccgaaact agaaaagctt tgataaagtg cacgacgata
      781 gagatggaca actctatcgt ttggcattga cagctgccca aatagctgag caaaatagtt
      841 caatgtttcc ttcagccatt tttcgatatc agattcctga aaagtcatta cttcttcaaa
      901 agcatttggg tccacaaaag caatggcgaa gcttgccact tcctgttgtg cgatcgtgtc
      961 cttatttcct ttttgagaaa tcaacacatt ttgctgatcc gaatacttct cttggtacaa
     1021 aggcacatct ttcactgaaa cttgaaggct ttcgtgtgtt tcattcacaa tgtaaacctg
     1081 ttgaacaagc atacttaaac gcttattagc cagtattgga gcaaaggata ctacaatgac
     1141 cttaaacgtc gaataatgat gaatatatct aggcaaacag tcttgaatca catcgacttc
     1201 aatacgatga tccgattctg acaaatggta gacaatcccg tttacttcaa cctttaagga
     1261 taacgaattg gtttgagaaa gctgtttatt gacccaaaca cctggttttt cctcagttag
     1321 taaattttct cgataaaagg tcaacgtttg aatcgtcccg caaacatcaa ggtttcctaa
     1381 tgtcttttga ttacttacat cgaaggtcaa tccctttaca tcaggaatat tccgatagta
     1441 gctgtattcg tttgaagtag tcacatttcc gtcgtttaac tcagcattaa aaaatgggtg
     1501 tttgggtgaa taaattactt cattaatagt catcttttcc ctccttgcta aaaaaactgc
     1561 agcctagtag ctgcagcttc ctactatgcg attccaagta atgacaataa aattcccaag
     1621 acgaccattc caaaaataat tttgatgacg ccgacttttt tatctagtaa tttgaaacag
     1681 ctcaatgtta ccaataacgg tacaattcct ttaaaaatac tatcgaggat ttcttggaca
     1741 ttcattagtt cttcgccctt cattgaaaag ctcaaaacgg tattgaattt gaccatactg
     1801 atcgtcattg cacctaccat gatcagaccc aagatactag cagcttttgt cagaatttca
     1861 attaacccgt tcgcatacag cttttcaata tatttagagc ctaatgaata gcctaaaacg
     1921 ccgccatagt atcgcatcaa gtaattaggt atattgaaca taactaaaaa gataattggt
     1981 gccaaaatat ttccggcatt ggctaagttg atcgctagtc ccgcggtaac gacacgccaa
     2041 actccccaga acaatgaatc gccaattcca gccaatggtc ccatcaaact cgttttgatc
     2101 gcgttgatac tttccacgtc aaaaccagct ttttcagaat tttccttctc catcgatgcc
     2161 gcaatcccca tgacaaagga cgcgcaagga atggtcgtcg aaaaatactg tgtatgacgt
     2221 tccagcgccg cagctcgatc ttcttcactt ttgtagaaat gattaatgaa gggcatcata
     2281 gaataggcga aaccaccgcc gcccatcgtc accggattca ccgaagcata catagtcatc
     2341 gagcgccaaa acatcttccg aaccaatttt ttctcatctt tagaaattcc atcagctaat
     2401 ttcattgaaa gaaatcctcc tcttcttggt caagcgttgc atttacagca gcatcacttt
     2461 gaactgcatg aacagatata ttttttagtt gtagatctct ttgggctata acaattgcaa
     2521 taatcgttcc aactgcggca attgcaatca atggtaactc gagatacgcc ataacaacgg
     2581 ccccaaagaa aaaccacaca gcaatatttt tactccaaag catccgtaac agcatcgcca
     2641 tcccaactgc cggcagcaga tttccagcga ctgttaatcc gttcatgatg acctctggaa
     2701 tattatccaa gacgcttttg acaacatctg ctccaaataa aacggcaaag aatggaataa
     2761 gagcataaag cgcatagtgg acaaaccaca taaagaagtg cagattgatg attccttttt
     2821 gatcaccaga ttttgcaaat ttgaaaaatt tagcagccat cggtccacat acaaaagaaa
     2881 aaatcatatt cattaactgt aatcctaata ctgctagtgg aaagacaatg ggaataatcg
     2941 catccacgtt tcctcccatt ttgattgcga acgctgtagc cagcccagca gccaaagaag
     3001 gctccgccgt actagagatt ccgatattga cgacacccat aaagatggct tctagtgacg
     3061 ccccgacaat caaccccgtc tgcaaatccc ccatcaacgc tcctgctaac ggagcaacaa
     3121 caatcggacg actcagcatg gacatcccga acatttcatg tccagcaact ccaataaaaa
     3181 cggccaaagc gactacaaat gcatcataca taatttttct ccttcctttt tctaaaaatc
     3241 actcaaaata gagatagctg aacgtttttc catcgccggt gtaggctgaa aaacggtatc
     3301 tggataaagt tcaactaatt cttttaagtc ttgaatttct gacgtcgtta acatcacttc
     3361 ctttgataag gttttcttat cttccgatgg cggtgtcatt ttcccagcat tccccacgtt
     3421 cattaattcg atttccgttg ggaaagcttt cgcaactttt accgcatctt tgacattagg
     3481 gactaaaacc atcaagcgca tgtccttcgc ccgcggatct tttaacaaat gaatcgcgtc
     3541 atcaactgtt ttaataatga ctttgacatt cgatggtgca gccatcttca atgtcatctt
     3601 ttgaatatcg ttggtagccg cctcatcgct tgctactact aacccggtta aatttagact
     3661 ctttgtccaa gtcatcgcta cttgtccatg aattaatcgt tcatcaattc gcactgcttt
     3721 aatcattttt cttcctccta aaaaaaatct tcctcttcat ttactacatg tacctcatca
     3781 agaatggtca tctttaattc ctgttggcat ttatttacca gttcctgaat atcctccttt
     3841 gttaattcct tggttgccaa tactatttcg agcagaattg gcagattaaa gccagacaaa
     3901 atagttacag caatctcttt ctccaaaaag gttttggtaa tcttctgatt gacacttccg
     3961 ccaaataaat cggtaaaaac aaaaatctga tcctgctcgt tcacctggtt tacaaataca
     4021 ttcaactcct gttcataatc acactcatcc acataagcat taatcaccgt aatctcttga
     4081 accttccccg tcagtaattg aatactgcta agaaagcctg aagctaagtg accgtgagtc
     4141 gctattagaa ttttcttcat atggttaatt cctttctttc tgttcacttt catataagca
     4201 attcctgtgc caacttttaa aaacattggt ttaatcacgt taattcacat aaaaaaaagt
     4261 gtctaacctg aaaaaatcag attagacact tttttagaca ctttattttt gaatatacgg
     4321 tcctaataat tcatagagca gcgaaagctc ataagcattg actttaaaat tatatttcat
     4381 ctccattgat tgaaaaatac ttctcgaaac ataataaaat tcatcttcat ctggtttcat
     4441 atcataattt ggttcttctg ctttttctgt ccgcgccaag aataaacgtt ccatcatcaa
     4501 tgccgtatgc atataaagat ttagttttac gcggccattt aaagtgaaat gatagtaatt
     4561 ttcatattta gaaatgactg tttccacctc actcatcacg attgttgggt tcagaaagtt
     4621 caaacggtct gcgattcctt ccaatgaaaa gaattttatc agctcctgaa tcaaacggtt
     4681 aaattctacc ggcttcatct cagaactcaa aatatgctt
//
LOCUS       DACDCJ010000141         4652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDCJ010000141 DACDCJ010000000
VERSION     DACDCJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.144"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..600
                     /locus_tag="IV038_002798"
                     /pseudo
     CDS             1..600
                     /locus_tag="IV038_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
     gene            634..2337
                     /locus_tag="IV038_002799"
     CDS             634..2337
                     /locus_tag="IV038_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP8342948.1"
                     /translation="MKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDN
                     QYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQT
                     VKNEPISKVQNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKE
                     LLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVK
                     GFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIF
                     PNRVFDFQLDQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFT
                     GEEEIVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMD
                     TSEKAIPYIATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPE
                     IQPAEVKQDEKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQ
                     HNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKER
                     V"
     gene            2398..3858
                     /locus_tag="IV038_002800"
     CDS             2398..3858
                     /locus_tag="IV038_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP8342949.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            3869..4312
                     /locus_tag="IV038_002801"
     CDS             3869..4312
                     /locus_tag="IV038_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAP8342950.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQ"
     gene            4388..>4652
                     /locus_tag="IV038_002802"
     CDS             4388..>4652
                     /locus_tag="IV038_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP8342951.1"
                     /translation="MIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIR
                     NFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEK"
BASE COUNT         1777 a          776 c          999 g         1100 t
ORIGIN      
        1 ttgggaaaat ccaaaagaaa attagtacga ttgcaaaatg aattaaatga tcaaatcgaa
       61 aagatgtttg aacaccaaag aaagaccaac ggtcaaccaa tgaacgataa gagaaacggg
      121 cattcttggt ttaggcaaca agaacgaatc gaaaataaag tccaaagtct gagagaagag
      181 atcaagcaac aagaaaaaca ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg
      241 ggttataaca agtacggtgg cctggatatg acgatcgaaa atattccaag aatcaaagaa
      301 gaaatcgaac gctttgaaaa aggtgaatcg actttttcag ctgccacgat ccgaaaatac
      361 caacgaaaat tggaaacatt agaacagctg aaagaaaaat cagagaaagg aaaagaaaat
      421 attcttcctg aagtacaagc gatcattgac agcggacgag tgacgcaatg gaaaaagaat
      481 ccaactatct attttctgaa aggctatcga aaagtcgctt tagaactcaa tgaaaacggg
      541 ggatttgagg aatctaaaaa ataccaagcc acgtcggaag aagaaaaggc agctgttcgt
      601 gaattacttg aagaagctac acctactgat tctgtgaagg agaaagaaac cgatcatttc
      661 tatcgagtag aattcaacga aaatgatcca gcaaaagggt tgaaaagtta tggtggggag
      721 atcgtaacac tagaattgat cgatcaactc aaaacactgg ataaccaata cgccgcagaa
      781 gcaggctatt ataaattcta ttttgaagaa gtgaagaatg gagaagtagt agatcagata
      841 cgaatggatc taggggatgg cgtcaatagt aatcacccca tctatttaga gttagctaga
      901 ttgtgcgaac aagtacctga gaaggaacaa aaacaaacag tcaaaaatga acctatttca
      961 aaagtacaaa atgagattaa agagacaaaa acaaaacaaa agggttatca aaaacgaacg
     1021 gcagaagaga ttaagcaaga agtcaaatca ctttcccaac aggcgcttga tgcggtgaaa
     1081 aagcatactc attcacctga agacgtcaaa gaacttttga attttatgtc tcgttttcca
     1141 gaacgatcct ttagaaatca attattgatc gaggaacaat ttccaggcgc aacggcttgt
     1201 ttaggtcgtg ccgcgttgaa aaaagaagga atctatatca aaaaagggga acaagggaac
     1261 aagatctttg tcagaaaaac ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga
     1321 gaaacagagg ctacaccaga agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc
     1381 aaaaaacctt actacacgat tgaaaaagtg tttgatatca cacaaacaca attgaagcct
     1441 gaagactatc cgaaaatctt cccgaaccgc gtctttgatt ttcaattgga tcaaacaggg
     1501 catacagaac ttcaagttgg gattcaagcg gtagccaaaa atattggaat tcaaattcga
     1561 gatatgacag aaagtgaagt ctatcaaaga gaattaggac aagcacgtgg ggcgtacgtt
     1621 cgtaacgaat ttactggcga agaagaaatc gtcatgaata ctcgaaatac tcctacgcag
     1681 catttagcca caagtattca cgaattagcc catgctagaa tgcacaaatt ttcagaatta
     1741 gatactgcaa caaaagaact acaggcagaa atgacctcct atattgtgtg caagcatttt
     1801 gggatggaca cctcggagaa agctattcct tatattgcga catggacgaa gaatggtcag
     1861 acgttggatg ataaggaagc tgcggaacgt gggaaaatca tgaatgatgt cagtcgtgtc
     1921 gcaaatgaat tcattcaaac catttctaat gaaatcaatc aatacagaga aagagaacca
     1981 gagatccagc cagcagaagt gaagcaggat gagaaaaatg ttgtagaaac agcagaacaa
     2041 aagaatattg tgctcgttgg ctcactttgg atcgacaaga aagacgggaa agtcctggaa
     2101 gaagacacgg gccgaacgtt acatttgaag gatgcggcgt ttattgaaga tacgcagcat
     2161 aatcaagtga ttttaaaagg aaaaagttat ggaaaagaga ttcaaacggc gttaccttca
     2221 gaagaatttg cgctaggaaa tccattccga ttttcacttg aaacagtgtc agaagtggcc
     2281 aaacgaaaaa atgaaatcta ttatgaacaa caattgtcac aaaaagaacg cgtataaaac
     2341 aagtaaaaaa gactatctga aaaacggata gtcttttttc ttttgggagg tttgaaaatg
     2401 accgagaaaa aaatgagtct tattgatcga tgtaaacaga tcgatatagt ggattttgct
     2461 cgaaacaatg gaatggctgt agtcaataaa ggacgagact atcgcttaga agaccatgat
     2521 tcatttgttt ttgatcgaag aaaacaacgt ttttattgga acagtcaaaa tattagtggc
     2581 gacattattg aattagctaa actttttttc atagacaaag agattcaaga ctctaagcaa
     2641 caatttaaag cagccacaga ttttattcta aagaatgaag acaaaacaga acgagtagaa
     2701 aatcttcatt ttgagacaga aaagtacaaa gatcaccccg ttgattatca accactcaca
     2761 gaaaaaggaa ggaattattt gaaggaagaa cgaaaactgc ccgattggtt gattgattat
     2821 gcagaaaaag aaggattgat cgccgaacta aagccaaaac atgaacggca aaatttctta
     2881 gttcaagacg atcgtttgga tcatgcggtc gcctttttat ggaaagatcc acagacaaaa
     2941 gagacagtag gggcgagtta tcaaggaaca ttcattgatt atgaacgttt tggtgagaga
     3001 ggcacatata agcatataga taaaaattcg acagcgaatc atggatttaa cctaaagatc
     3061 ggtgacccta aacagttaaa attttttgag agtagtattg atttacttag ctatgctgca
     3121 ctgaatcgtg atcagttaaa cgatacgtgg ttagtctcta tggaagggtt aaaacaccat
     3181 gtgatcagtc attattttgg tgaggcagta tctgaattga gaaagaaaca agcctttcct
     3241 caatccattg agatttgtgt ggataatgac cgtgcaggac atattttcta cgaaaaagaa
     3301 caattaatgg gcgcagttga tccatttacg aatcagaaag tacgttgtga acgaggaatc
     3361 gctaatgatt ggcaagtgcc aaaagaatac aaagtcattt atgaagaagt tgcaaaagaa
     3421 atgaaagttg aacctgaagc aattatggcc atacacaaga cagaaaacaa tctgcaatta
     3481 acgaaccaac tggtttccgc tcacaaagta aatgcgtctt ttggtcagca gctttctgtt
     3541 aacgattcaa tagaagctat taatttgaaa gatatttgta gagaagtcgc aaaagaatta
     3601 aaaggttgtg aacgcgtgga tggtacgtac gattttgatc gtttttatca agaaaaaggg
     3661 gatattaatg cgcagattct tttctcttat aaagcagaac aatactacaa aggctataaa
     3721 aatcatgaac atgaatttgt acctgaagta aaaaaggatt ggaatgatca attgaagcat
     3781 gaaatccacc aacaagagat cagaaagcaa aaacgtgcga tgttgtttca acaaggcaaa
     3841 caacaagaaa gggagtgaaa tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc
     3901 acaagcgaaa gcttatgccg atagcttctc taaagccacc cgaaaagacg ggtattttga
     3961 aatccaagat agattgtttc ctggtgaaac tgtgatcacg tatggttttg gtcatttagt
     4021 cgagttagat tcgcccgata tgtacgatga aaattggaaa caatggtcat tagagcattt
     4081 gcctattttc cctacacatt atcactatca tgttcctaaa gataagaaaa agcaattcaa
     4141 ggtggtgaaa cagcaacttc aatcggccga caccattatt attgcgacag atagcgatcg
     4201 tgaaggggaa ctgatagctt ggacgatcat tcaacaagca ggcgccgatc atggaaagat
     4261 atttaaaaga ctttggatca actccttaga aaaagaagcc atttatcagt gattccagca
     4321 attacgagac gcagaagaga cctatcccaa atttgaagaa gcacaagcac gccagatcgc
     4381 tgattggttg atcggcatga acggcagccc actgtatagt ttactcttgc agcaaaaagg
     4441 cataccagga agtttttcct taggcagagt ccaaacccca acgctttaca tgatttatca
     4501 gctgcaggaa aagattagaa acttccaaaa agagccttat tttgaaggaa aagcccaagt
     4561 aatcgctcaa aatggcgcat ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca
     4621 agaagcattt gaagattatt taaaagaaaa gg
//
LOCUS       DACDCJ010000142         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDCJ010000142 DACDCJ010000000
VERSION     DACDCJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.145"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IV038_002803"
     CDS             574..1008
                     /locus_tag="IV038_002803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342952.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IV038_002804"
     CDS             2298..3221
                     /locus_tag="IV038_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8342953.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IV038_002805"
     CDS             3237..3776
                     /locus_tag="IV038_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP8342954.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1668 a          671 c          755 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDCJ010000143         4414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDCJ010000143 DACDCJ010000000
VERSION     DACDCJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.146"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IV038_002806"
     CDS             complement(37..1257)
                     /locus_tag="IV038_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8342955.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IV038_002807"
     CDS             complement(1277..1882)
                     /locus_tag="IV038_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP8342956.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IV038_002808"
     CDS             complement(1931..2863)
                     /locus_tag="IV038_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP8342957.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IV038_002809"
     CDS             complement(3284..4144)
                     /locus_tag="IV038_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8342958.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
     gene            complement(4291..>4414)
                     /locus_tag="IV038_002810"
     CDS             complement(4291..>4414)
                     /locus_tag="IV038_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8342959.1"
                     /translation="STTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1254 a          888 c          729 g         1543 t
ORIGIN      
        1 tttattcttt atttaaagct ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa gatatcggtt ctgttgcaaa gttttaaata
     4261 aagaataaaa tcccttacgg tatctatgat ttaagctggg attcccaata ataccttgat
     4321 ttcagtacag accgaaaacc cgaagagagt gccttctttt cgggttttct tatataatcc
     4381 tcgaatggct tccatgcctt taatcgtggt agag
//
LOCUS       DACDCJ010000144         4277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDCJ010000144 DACDCJ010000000
VERSION     DACDCJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.147"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..182
                     /locus_tag="IV038_002811"
     CDS             <1..182
                     /locus_tag="IV038_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342960.1"
                     /translation="ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRN
                     SSYGSDYTFAITYTI"
     gene            345..791
                     /locus_tag="IV038_002812"
     CDS             345..791
                     /locus_tag="IV038_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342961.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            793..930
                     /locus_tag="IV038_002813"
     CDS             793..930
                     /locus_tag="IV038_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP8342962.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            968..1261
                     /locus_tag="IV038_002814"
     CDS             968..1261
                     /locus_tag="IV038_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342963.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            1258..1482
                     /locus_tag="IV038_002815"
     CDS             1258..1482
                     /locus_tag="IV038_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP8342964.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1479..2504
                     /locus_tag="IV038_002816"
     CDS             1479..2504
                     /locus_tag="IV038_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP8342965.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            2693..2766
                     /locus_tag="IV038_002817"
     tRNA            2693..2766
                     /locus_tag="IV038_002817"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2727..2729,aa:Ile,seq:cat)
     gene            3443..4277
                     /locus_tag="IV038_002818"
                     /pseudo
     CDS             3443..4277
                     /locus_tag="IV038_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1468 a          738 c          864 g         1207 t
ORIGIN      
        1 cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct
       61 cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta
      121 aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt
      181 agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag
      241 caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg
      301 attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga
      361 aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg
      421 ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa
      481 tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag
      541 atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg
      601 atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata
      661 ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt
      721 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg
      781 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttc
      841 actaacgaac aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa
      901 atcattagca aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag
      961 ttgtcacatg attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt
     1021 ttctttgatt ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac
     1081 tctttcaaaa gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc
     1141 agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc
     1201 agaaaaattg gtagaacaca cgcaacaaat taaaacttta tttagggaga gaagaaaatg
     1261 aataataaaa cgttcgaagt actaaaatgg ttcgcactgg taattattcc cgcactagct
     1321 actttcgtgg ggttagttgg taaagcgctc aattggcagt acacagatat ctgtgttgtc
     1381 atcattactg gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa tcgaacctac
     1441 aaaatgttct cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa attactatta
     1501 ctgcgatgag cctgttaacg gctctttttt tattacccat taatacgttt gcttatacta
     1561 ttaatgacga gtataattta gcgccgaatc aaggagactc cagattagca attcctaaca
     1621 aaattatttt gcacgaaact ggaatagatg caccagcaag aaacgtagcc gccaacatga
     1681 aaaataatta taacggaagc aattcttata ctacagatgt tattggtgac ggtgggattg
     1741 tttaccgtgt gggtgagcaa ggatatgttt cgtggggagc tggtaacgct aatccttatg
     1801 cgcctgtaca gattgaatta cagcgcacat atgataaagc attgtttgaa aaaaactatc
     1861 gagcttatat tgaatataca agagatagtg caaaaaaata tggaattcca ttgactcttg
     1921 atcaaggaac ttctttattt acaaaaggaa tcatttctca tttgtgggtg acaaattatg
     1981 tttgggggaa ccacacagat ccatatggtt acttatcgca aatgggagtt agcaaagaaa
     2041 agcttgctta tgatttagct catggattta ccgatgaaaa tccaacaact tctgaaaaca
     2101 agcctgtcat tgatccaaca cgagctggtg cagctaatcc tacgctgaca gatggaacaa
     2161 atcacgccca cattgatcag tttggggaaa tcgaaaacgc aaacttgcat gtggctggat
     2221 ggcacattgc taactataaa tacgagtata ttttcattat ggactacaat actgggaaag
     2281 aattagctcg agtaagagct gatggaattt atagaccaga tgtaaaccaa gcttataata
     2341 cttctggaaa tgttggttat catgtatctt tcaatatgcg taattttcct agtaagaaag
     2401 tctatgtaat gatgcgggca acgaatgatc cagagggaaa cactaaaggc ggtgcgcaag
     2461 atttccatga caaacgttgg tatttaaata ttcctaaacg ataaaaatag ctcctcgttg
     2521 aggagcagta cataactata ttgacaacta taaaaattat tcgataaaat agtgatgtta
     2581 tcgcatatct tcactatcac ccataatagt cacactccaa gctatgcgat aacaggtttg
     2641 ttgccacaca ttctactggt tgattgttta tggctttatg tggcaacaac cagtaccctt
     2701 agctcagttg gtcagagcag acggctcata accgtccggt cgtaggttcg agtcctacag
     2761 ggtacattaa cgtagccatt tgaatcgttc tgtgttagaa tttttttgaa gagtattata
     2821 caagctaaag cttttcttca ttgccactca aatgagtggc ttttttatgt atccttttat
     2881 ggattaatga aaggatgttt cacatagtta tacttctgta tatttgaaaa gttttacttt
     2941 gatttttaaa tagaaagaca tttgggttaa attgtgagat aataataaag aagagtttaa
     3001 agcgcacccc aaaccacttc cccataagtg tgttacgctt taaactcttt tatatttgaa
     3061 gccattaaaa agcataccat atttttgaaa aaaagtgaga aaaaaggctt acaattggag
     3121 tggtagttaa ttagtgactt atttttgatt ttatagcact gatactataa aatatagata
     3181 tcatcatatt acacaatctt aatactaact taaaaaatat ctcctttcac aagtatggtg
     3241 ataaaattcg ttccgggcta cctttttagg tagcctactt taatctttat acctttctgg
     3301 atcaacgaaa gtatacttta tatagtcata acgccgatga tcgctacgtg cgtccggcac
     3361 gtcagtcatg aatcggctac actagactag acagaaaaaa taaggtgtgt agaataataa
     3421 aaaacacact ggaggatttt tcatgtcaag acgtcaacga agaacctatt caaaagaatt
     3481 caaacaacaa atcgtcgatc tctatctcgc tggtaagcct cgcgcagaaa ttattcgaga
     3541 gtatgagctt acgccttctt ctttcgataa atggatgaag caagcacaat caacgggctc
     3601 attcaaagaa agagacaact taacaccaga acaagcagaa ttgatcgcac taagaaagaa
     3661 aaataagcaa ctcgaaatgg agaatgatat tttaaagcaa gcggcgctga tattcggacg
     3721 aaaagacaag taattgatgc caacaagcat aaatattcca tatcagcgat gtgcaaaatt
     3781 ctaaatattt ctcgtcaaac ctactattat caagcgaaac cgatcgaaaa tgagtccgac
     3841 ttagaagaga tcgttcagga agagtttatt cgaaaccgaa aggcttacgg tacccgaaaa
     3901 ttgaagaagt gtttagcaaa gcgtgggctt caactcagtc ggcgccgaat cggtcgaatc
     3961 atgaaacgcc gcggattgac atctacctat acgatcgctc attttaaagg gcaacgaaca
     4021 gcttgtaatg aagcgaaaac agcgaatgta ttagatcgga cctttacaca agaacagcca
     4081 ttggaagcca tcgttacgga tcttacttat gttcgcgtgg ggaaaaagtg gcattacatc
     4141 tgcttaatac ttgatttgtt taatcgagaa attattggtt attcctgtgg tgagaagaaa
     4201 gatgcctcat tggtaaaaga agcctttgga cggataccgt attctttaac agacgtcaag
     4261 ctttttcata cagaccg
//
LOCUS       DACDCJ010000145         4147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDCJ010000145 DACDCJ010000000
VERSION     DACDCJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.148"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..388
                     /locus_tag="IV038_002819"
     CDS             80..388
                     /locus_tag="IV038_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342966.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            392..754
                     /locus_tag="IV038_002820"
     CDS             392..754
                     /locus_tag="IV038_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342967.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            975..2381
                     /locus_tag="IV038_002821"
     CDS             975..2381
                     /locus_tag="IV038_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8342968.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            2828..3802
                     /locus_tag="IV038_002822"
     CDS             2828..3802
                     /locus_tag="IV038_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP8342969.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIACDIVDIPDDFVSQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRESFAESADDLKRELDTWLQGLDVTEVGAE"
     gene            3799..>4147
                     /locus_tag="IV038_002823"
     CDS             3799..>4147
                     /locus_tag="IV038_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342970.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQ"
BASE COUNT         1445 a          602 c          928 g         1172 t
ORIGIN      
        1 aaaaaaacga tcagttgtta ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat
       61 tctggaaatg aggtgtgaca tggataaaat tgatttgaga gaagaaacag tcgatagaat
      121 tgaattgatg tatgaacggt tagctgatta tttgaatttg ccggatgaag ataattatag
      181 tgaaatgctt gaacgaatta tggatgttct caactcggtt atcaatgctg atttagcaaa
      241 atcttcagtt ttgactaatg aatttgttca taagcattta gcgcaaacaa ctgatgaatc
      301 agaaaaagaa ttttggttga aattaatgag tcatgttatg cagttaaacc aaatttccgt
      361 tattaattcc aaagaaaagg agatgtagtt tatgggaatg actttagcag atattaaagc
      421 gcaagaagaa aaattaaaaa aagaacaagc gaaacttcgc caacagaaaa aacgcttgat
      481 tgagaaagaa aatgctgcaa tgggcaaggc tcttcgcaag cgttttgggt ttgaaacttt
      541 tgctgatttt gagcggtggc tatcgtcgat ttctgacgat cagcaacacg aaaaatcagt
      601 ggataatgag tggcttgctg atgaaaaatc agcgccaagc gaacgagatt atcccgatga
      661 atctgacggc ttattcggag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
      721 tacgtcaata tttaatgacg tgaatttacg ataagcgatt attttgcgtg aagcaaaata
      781 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
      841 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
      901 tgttactctt tgacaaagag taacagaaag gtttacactc tttttattag cgacagccga
      961 aggagggttt taacttgggg ttcagtattt cgttcaaaaa gggaacaaag aatacaagca
     1021 tttctcataa taatcgtgag ttaactgata gccaaaaaat caatgactgg cataagcata
     1081 ttgatttcac aaaatcggat gaaaatatct atttagaaca aacaaatatt cgtgaaaaat
     1141 atgaagaatt attcggtgaa gccgtagaaa aatataatgc aaagcagaaa agagccgata
     1201 gaaaaatcga ggactattta gcaaaggttc gcaaggataa aaaattggaa ccacagcgag
     1261 aatttatcgt gcaaattggt actcttgatg attttcgtac tactcgtgat gatggttctt
     1321 ctactggcat atcggaacaa caagcagaac aaaatcgagt gattgccaac aaaattttag
     1381 ttgagtattt caaagaattt aaagaacgga atcctaattt atccgtctac aatgctgtta
     1441 ttcataatga cgaagtaagt ccgcacctgc atttgaatat tgttcctgtt gctgaagggt
     1501 ataaacgggg tgttcaaaag caaccaagtt ttaacaaagc cttgcaacag cagggtttac
     1561 gagcagataa agagaatggt cgagtgattt ggaataattt taggaatcag gaagttgagt
     1621 caattgaacg tttgatggca gattttggtt gggagcgtga gttagtcggt actaacaaaa
     1681 tcaaagatgt tcacgaatat aaagaaatca tgagcgagat ttcagaattg cgtcaaacgg
     1741 ccgtaaaaga gcgtgagagc gtttctgatg agttgaggta tatttctagg caaagagaag
     1801 aaaatgagcg agaaaaggct ctggtggctt ctgagagagt gaagattgaa gaagagaaga
     1861 aaaatatgct gaatgtaaaa aaagagatgg cggtcatttt ccaaaaagat attgttcctt
     1921 tagaaagttt caagaggctt cctgatggtt cgtatcattt atctgggaat gattttgttg
     1981 atttgtatca acgagctacg agagcaacaa atgccaagag aaatgctgat aacatgagtg
     2041 agcaatattt acagatgctt gagcaaaata cagaattaaa tgagtatgta gctacattgg
     2101 aagaagagcg tcatttgtct ggtagaaaac aattagcaga tttacaagtg gaaaataaaa
     2161 agctgaaaga agaaaatcgc tctttacgcc agcgtttaga gcgattaaaa aacgcatttc
     2221 aaaaatcgat cactcgtttg tctatccgtt ttggtcttgc agaaaaggat atggggcttt
     2281 ctgaggaatt agaagtgtta gagcaggaga aaaccttatt ttatcgtcaa aacgtctcac
     2341 agcgtaaatt agagcgttct gaagatgaga tcgagtggta gttacgatgt gcagtcaacg
     2401 gtaccggagg cggtttgtgc cgaggataag gagttgacgg ctaaaccact agcctattaa
     2461 gctgacattt gctaaaaaat ggccagtcca ttttttggcc aacctagcgt tagcgaagtt
     2521 tgggggcgcc cttttcaaag ggtgcggttt tattttttgg atatttgcat agctaaagag
     2581 aagtgctatt attgagttag caaacaaatg aactgaaaaa agagtaaaaa aatagtcgag
     2641 caacgctacc aacgttctcg acctttacgg aaactaatcg cactagctcc gtaacttctt
     2701 aatcattata cgaatatttt tagatttctt caagactaga gattttttgg taatgaattt
     2761 ttaagagtta cgtgaacttt tgaaaattct ttccagaagt ctctttttct ttgaggagga
     2821 acaaaatttg agtaaacgtg gcacgatcgt gaatgagtgg gaaaaactta accgcatttc
     2881 taacaggcgg ttaaagtatc aaagtttgac ccttccctta aaaatgcagt ggtcaattga
     2941 ccgaattacc atagtcggaa aactgaaaga aaatatttat tatcacactc caaatgatgt
     3001 gttgatttta aattttgaac agcttatgcg tttgaacgaa ggcaatggtt atttaaaagc
     3061 tgttgggaat aatgggtggc aattattaga ccaacacgag gaaaatatag cttatattga
     3121 gattcttaaa tttcatgaag gaaaagggcg gattgacttt aatcctaata aaatcaatca
     3181 atttttatca ggttccatga aaaatttcat tcatgattta tttctagaac ctcatttttc
     3241 acgtgcggat attgcttgcg atattgtaga tattcccgat gactttgtta gtcagtatcg
     3301 tgtagttgat cctgtgtctt ttaaaccaat ttatgggcgt tctggtaagt tagaaacggc
     3361 atattggggt agtcgtgcca gtgaacgaca aattcgaatg tacaataaaa aattggaaca
     3421 agaaagaaaa cgtaaaattg ttccgccgga aataacgaca tggtggcgac tagaattgca
     3481 gttacgaagg ggaaaagcaa ctgattggca tgagatggtt catgaaagtt tggatagctt
     3541 tgctagtcca cattattttc cgccaaatac taaagttact gaacggatta tggtgaaagg
     3601 tttgctttat gatcattctg agtggggaaa tctagctaag aatacaaaga aaaaatacag
     3661 ggatttgttg aagcaagaaa gtcaaaatga tgaattgacg aatcatttgc gagagtcatt
     3721 tgcggaatcg gcagacgatt tgaaacgaga attagataca tggctccaag gacttgatgt
     3781 aacggaagta ggtgctgaat gagtaatcca gtagaaaaaa tgaccataaa aaatcttgct
     3841 gacgaattgg gtgtatcgaa aaatacagtg cgcaatcaac tggtcaattc agggtttgac
     3901 cttgctcagt ataaagaaaa tggcattatc atgctagata aagagttgat tggtgttgtt
     3961 cggtcaattt atcggtcaaa aagtgatcaa ttgactggtc aattaccggt caataacaca
     4021 ggcagtgatt ttcctttaga aaaagagaat gagtttttgc gtgatcaatt agctgagaag
     4081 gacaaacata tttctgaatt aacagaaatg attcgtgatc aacaaaaatt gaccctgctt
     4141 cagcaac
//
LOCUS       DACDCJ010000146         4123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDCJ010000146 DACDCJ010000000
VERSION     DACDCJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.149"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..439)
                     /locus_tag="IV038_002824"
     CDS             complement(<1..439)
                     /locus_tag="IV038_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8342971.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNI"
     gene            496..1623
                     /locus_tag="IV038_002825"
     CDS             496..1623
                     /locus_tag="IV038_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8342972.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1841..2983)
                     /locus_tag="IV038_002826"
     CDS             complement(1841..2983)
                     /locus_tag="IV038_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8342973.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2988..3173)
                     /locus_tag="IV038_002827"
     CDS             complement(2988..3173)
                     /locus_tag="IV038_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP8342974.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3527..3772)
                     /locus_tag="IV038_002828"
     CDS             complement(3527..3772)
                     /locus_tag="IV038_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8342975.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3766..>4123)
                     /locus_tag="IV038_002829"
     CDS             complement(3766..>4123)
                     /locus_tag="IV038_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8342976.1"
                     /translation="AYCKKVLRNEAKSIRKRNANIKDNEEPLADSNEGRYSQTHFDEQ
                     DVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDAEIGKILNMSTSGIWY
                     QRKKAVEQLKMGYDLW"
BASE COUNT         1313 a          753 c          652 g         1405 t
ORIGIN      
        1 aaatatttct tttgaatgcc tgttttccac gtttttcctt ataaccattt ggttttcgtt
       61 ttccttgcat cggtaaatgt aaaacattga attctcccgt cttaaaccgg cgatagaggg
      121 tacttactga acaagagaag gttcgctctg cacggccaat aatgacgtct ggggtccaac
      181 cttcagttga tctttttgca atgtattctt tttcctcagc aggaaaaatg atttcggttc
      241 tcccacaacg tcgcttattt tctttgtatt gttcaaagta ttcaagtgct gttccaccac
      301 atttgagaaa gttatagaca ttgtaaattg tttggcgtcc acgtttaagc tgcttcgcaa
      361 cgatagcaac cggagtttct tgatgaaaat atgcttctat cattacaagc tcgtttggtg
      421 taagatgggt ataagtcatt tatgttcact ctccttgtat gctttagcgg gtattacaat
      481 tggagtgtaa cataaatggc ttttttattt gtctcgctta attatacaaa cggcgaagat
      541 atcaatgatg atggctcaag aaaaaaagtg gagaaagttg gcggagacac ccgaccagag
      601 gccgaagctg ctttacgaaa agttttatca gatattgacg aaacaggaca atacttttta
      661 ggtacggata ctcgagtaaa acaatacctt gatttttgga tggaggaata cgttaaacta
      721 aatctaaaat acaataccta tgaaaactac cgatttacca tcaaaaatca tataaacggt
      781 tatttaggaa agaaaaaact tacggatctc tcccctgctc ttttacaaaa tttcatcaat
      841 gctgaattta aaaagggtta ctcgaagaaa acaatgacta ttactcactc tgtccttaag
      901 aatgcgctga atatggcggt ttatccttgg ggattaatca agcaaaatcc tatgctgtat
      961 gtaaaaatac caaaatatga agcacgacca acgactaaaa aagatctaaa aatcatttct
     1021 cttgaggact ttgatcatat gctagaaatt actcctgaag gacatccttt ctatattcct
     1081 ttgaatattg gattttatac gggaatgcgc gttggcgaag tttgtggtct gacgtgggat
     1141 gatgtcgatt tttcaaatgg aacaattact gtagagaaac aaatggtaaa gaatgacggc
     1201 gcatgggtat atggtacgcc aaagacaagc agttccaatc gaacgatttt tattggacaa
     1261 accttgctag caattctgaa aaaacataag aaacaacaat tagaaaatcg aatgaagtat
     1321 ggaaagctct acattgattc aaatgcagta tgtacgaagg aagacggtga gctagttacg
     1381 ccaagtgtag tgaaatggaa tactaggagg atatcgaatg cactctccct ctcttttaac
     1441 ttccattctc tcagacatac tcatgctaca cttcttctcg aaaatggcgc aaaaatgaaa
     1501 gaaatctctg aacgattggg ccacagcaga atttcaatta cgatggatac ttactcgcat
     1561 gtaacagata agatgagaaa tgaaacggtc gatatcatgg aaaatcttag aaagaattct
     1621 tgatttttgc caccgaaaaa aaccaccggt ggcaaaccgg tggcaaatcc atgtaaacat
     1681 ggtttatttt ttacactgtt ttcaaacaaa cccttgctat ttcttcggtt cgtcctcgtc
     1741 cattttcgat accgacagct agcttataac ccaaggtata tcaacataaa aaaataaaaa
     1801 taggctatga cagcagcgtg acagcaaaaa agatggaaag ttaaatatca aacctcgtaa
     1861 tttcatttgc gagtgcttga tttgtttctg gatataagtg tccatatttg tccaaagtag
     1921 ttttaataga ggcatgacct agacgcttag atattgcata gatattttta ttatgttcaa
     1981 ttaaaaaggc aacatgcgag tgtcttaatc catgcatatg aattggtttt actcctgcaa
     2041 tttcagctag tttttttatt ctgcttaaaa tagttcttgg agatggcggt aatccatcaa
     2101 aagaaaaaac caattctata tttttcccaa ttgattgttg agtttttttc catgttttta
     2161 aatcttcaac ggtttgtctg tctaacgaaa ttatccgaat acttgatgta ttttttgttt
     2221 cagcaaattc ataatcttgt cttgtacgga tgtaaattga cttatttacg ctacatctgc
     2281 catttattaa gtcaaagtct ttccattgta acgctaagag ctcctcactc cttacacctg
     2341 taacaaaaat aaaacgcatc aaacgtttat aaaattcttc ataaaaatcc ccgtgataaa
     2401 cactattata tactttctta aattcatcaa ctgtccaaaa ttctacttga ggacgctgag
     2461 tttttatttt acctatagta tctacaggat tttcttcaat aatttcaagt acaattgccc
     2521 ttttaaaaac tactctcaac ttattgaata tctgtttaat ataatttggt gataatggtt
     2581 gctcaatgcc cttttttaca acatggattt tcgataaaaa ctgttgaaat tcctgtatat
     2641 gaattggtcg aatctttaca acctgcattt ctccaaaaaa atccaatgca cgttgtaaaa
     2701 tatgatctgt tttcatatat gttttttcta cagttcccaa cttataccaa ggaaaaaaat
     2761 actctcgata aaaatcatga aacgttattc cttgcagata aattccttgt gaatcagaaa
     2821 aactttgttt taactcttcg agtccccttc tagcttctcc ttgagttttg aagcctcttc
     2881 tggtcacttg aattcttttt ccaaagcgat cttgtcctaa agttgcagaa aaaaaccaag
     2941 tgcctctttt tctgtcttta taaacattcg caattttcct cattttacta ttcctcccaa
     3001 gaaatatgga gaacttcttc taaaattgaa ataggaattc tttttatttt tgaattacga
     3061 taaaaaaagt atcctttctc aactaaaagt tctttacatt ccttgaagat tttattagca
     3121 tagtactgag aaaaacctag attaatcaaa tcttttcgag tagctgtttt cattatagca
     3181 ccttccttac tttttttcat ttatttctat cagctgcttt tgtcaaaagc acataggaat
     3241 ttcacctctc cccaagttta cggcgagcct tacggaagta tcattatccc aactttaaat
     3301 catcgctttt tattgactat aaaaattatt ttagaatatt tatcgctcct tctttacagc
     3361 aagaaatcat ggcgcaacta aaatatgctc cttaaagtgt taacgtactg atagaatcta
     3421 tttaattttc aatgaacaat aggttttcta cctaaaagaa tctactaatt ccttaactat
     3481 tagaagattc ttttaaacag aaaaagtgga aaggggattt ttttaattag ttaattttaa
     3541 attttaagat cgcttctatt aatttaattt ctagctgact tatcatatat tcatctacat
     3601 aggtaacgaa ttgacctacc tcgttaaatc catttgtcaa acataactta gccatataag
     3661 aatgataatg tttgagtatc atcttgatcg aaattggatc tccttctaga gcagataaga
     3721 taatggatag tggaactaat aggtactgcc gctttttaga tttatctacc ataggtcata
     3781 ccccattttc aattgttcaa ctgccttctt tcgttgatac cagataccac ttgtgctcat
     3841 attgagaatt ttaccgattt cagcatcatt aaaacctgca aaataataaa gtaggataat
     3901 ctttcttctt atctccgata actgatcaat tgctgctgat aacttttgat ctgaaattaa
     3961 tatttccatt ccaaataaga aatagacatc ttgttcatca aaatgagttt gggaatacct
     4021 gccttcattt gaatctgcca atggttcctc attatccttt atgttagcgt ttcgttttcg
     4081 aatagactta gcttcgtttc gtaatacttt tttacagtag gca
//
LOCUS       DACDCJ010000147         4040 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDCJ010000147 DACDCJ010000000
VERSION     DACDCJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.150"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            180..776
                     /locus_tag="IV038_002830"
     CDS             180..776
                     /locus_tag="IV038_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP8342977.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            900..2699
                     /locus_tag="IV038_002831"
     CDS             900..2699
                     /locus_tag="IV038_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP8342978.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2977..3189)
                     /locus_tag="IV038_002832"
     CDS             complement(2977..3189)
                     /locus_tag="IV038_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342979.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1187 a          688 c          712 g         1453 t
ORIGIN      
        1 ttttaaaaaa aacaagttat taaataagtc tttagttgga tgtttttttg agatcaatct
       61 tttatactta tgatgaactt acaaaattgt ttgttttctg tcatatcaaa aaatggcgca
      121 gttagttgct tttctagtaa agtatagtta acaaagaacc tctaaggagt tgatgaataa
      181 tgaatattgc tgcctctcga aaactgataa atttcatatc cattatcgga ttaggcctat
      241 caatcgcgct gacgatttat tttattaatt taggtgtttt caaagatttg aatgcgttaa
      301 gaggattagt aggtgattcc atcattttag gaccgattat ttttgtcttc atccaaatcc
      361 tgcaagtagt tattccaatc attcctggag ggatcagtac tgcagctggt gtcctgatat
      421 ttggccctta tgctggattt atctataact atattggaat ctgcattggt tcgattatca
      481 tttttctgct agggcgtcgt tatggaaagc cattcatact ttccatgatt agcgataaga
      541 catataacaa atatattggc tggctggata atcaaaaccg ttttgagaag ctgtttgctc
      601 tagctatttt tcttccagtt gctcctgacg atgcgttatg tttgatggct ggattgacaa
      661 acatgtcagt gaaacgatat acactaatca ttttgattgc taaaccactg tcgattttct
      721 tatatagtat ggccttgatt tatggaggtc aatatctaag tggtcttctt ggataatatt
      781 tattttgtaa aattagtcat tgattgacaa tgttaaatct gggaaagggt ctgcaaaaca
      841 cactgcaccc tttccttttt cattagattt gcgctataat gtgaagagca ggtgatagga
      901 tgaattattt gaaacgtagc ttaggtaatg cttgggactt tttaaaagga acacaagctt
      961 atttccgtga tgtcttgctg atgcatggct ttattttatt tatttgttta cccttattga
     1021 gcagtacgac ccgttttatt ttacaccgag gggcaattga ctatttgtct tatgacaata
     1081 ttccttcaat tttttctcaa catcctgggg ttctactctc gttaatcgta gttttgatga
     1141 cgattttact gttagtttat ttcgaattta cttttctttt gatgagtgtt ttttttatta
     1201 aaaagaaaga accaatctct ttgaagcaac tcttacactt gacgatactt cagctgaaga
     1261 aagtgagacc cattactttt ttgtttttcc ttgcttattt tttattgatt ctaccgatca
     1321 gcggattaag ttttaattct gatctactgt caaaaataaa aatccccgct ttcattatgg
     1381 attttatttt tgctaatcgt tggatcatcg tcagttcctt tttactcgtt tatattttcc
     1441 ttggctatat tgggattcga ctgatttttg ctttaccgga aatgattcta cgggatcgtc
     1501 cttttcgagc agccattcgg gaaagctggt cattgaccaa atcgcgtctt ttggccatca
     1561 cgggccaatt tattgtgatt ggcggtacga ttttactcct ttcttcttta ggatatatcg
     1621 ttgttatttt ggcacaaagt atggttgaac aatttttccc agactatgca ttgatcagcg
     1681 ctgtgtttgc tatgacgctt ttacaaggga ttttattatt caatatcgtg atgtctaccg
     1741 tagggatttt ttatatcatt gttgatttca tggatgacga aggcttcttg cctgagatcc
     1801 ctaagtggtt tatcccacaa gcccccaact tgcgtttctc tgcgctgaaa aacactggtt
     1861 tgactctttt tgcagttttt ttcggtattg gcgtctgtct ttacaatatg gattatctga
     1921 cttcagctgt tcagacaaaa ccagtgactg tctctcacag aggtgtgagt acccaaaatg
     1981 gggcgcaaaa tactttagaa gcacttgaaa aaacaagcag agactatcat cctgattatg
     2041 tagagatgga tgttcaggaa acaaaagatg gtcatttcgt agtgatgcat gatgcaaatc
     2101 ttcgtcactt aacaggggta aatggaacac cacaggatct tacattgaaa gaattgacaa
     2161 atcttacagt tactgaaaac ggagtgcaag cccctgtttg ttcttttgat tcttatttgg
     2221 aaaaagcaca taagctaaac caaaaattac ttattgagat caagacctct aaaaaagatt
     2281 cggatgacct tgtcgaacga tttgtccata aataccgcaa agttatctta gagaaccatc
     2341 atattttgca aagtttgacg taccaaaccg tttcagattt aaaagaagca gaaccaaact
     2401 tttatgtggg ctacatctta cctttcaata ttgttgggcc ccctgtgacg cctgctgatt
     2461 ttctgacaat ggaatatagt acgatcaacc gtaacttcat taattctgcc caccaagacg
     2521 gaaaagaagt atatgtatgg acaatcaacg attcagatgc tatgagtcgc atgatgtttt
     2581 atggtgtgga tggtatcata accgatgaga tgggactact aaatgaaagt gtgaaacaaa
     2641 cagatgaaga aatcacttat tcagataaac tgctaaattt tgttttggga gtcggctaat
     2701 caaaaatcgt taatgaaaaa agtctttata caacaaaaaa cgcataacct gccaccgcag
     2761 gtatatgcgt tttttgtttg acgctcataa tgtgtgctta actttctctt gattccttca
     2821 tcgcacgaac aatagctaaa atagcaggaa ttgcagagat cactgcaaga ataattccca
     2881 ttttccctga actttttctt tttggcatat cttctcgctc ccttctgtct ttatttatat
     2941 tataagggag ttcttgcgaa ttctcaaaaa tactgtttaa gaaaccaatt cagcaggtgc
     3001 gctaatatca cagaatttta cgagcatgcg atacttgctt tcattgataa gttgatagtc
     3061 tcgttggtca taactttcaa catacacgac agccgtgttg atatatgtac gttcaataca
     3121 gccgattagt tcatgagata ctccgatcgg tttgcatata taagatcttc ctacttctgc
     3181 ttttaccatt tcattccctc ttttctttat ttttattttg ttttttcttt attttataat
     3241 aacaaattac cttgtatttg aaaacgtttt tatccatttt tttatttttt attttgagta
     3301 tctttctgct ttttagataa ctatttttta acacgatctt agacgttgtg acaaaaacgt
     3361 ttgtttatat tcctatgttg ttcgtgcgtt tttttcttta tattgctaag ttatttataa
     3421 agaggtgagc atatgattta tattcaagca atagtatttg taaccttgat gattgggcta
     3481 cttatcggat taaaagaaaa tagctaaacc atttaagaaa aaataaataa ggaaaatgat
     3541 aaatgctttt taattgcatc aaaaaacgta cgatgctaaa cttggtataa gttcgaaagt
     3601 ttaaaggaaa ctttcaagaa ctaaattaca ttacaaagga gatgaatttt atgacgttta
     3661 caaaaacaaa tatgcaatac cgcgtagctt ttgatacaac ttctaaccgt tttatggcga
     3721 ttgatgcaca aaacgaaaat aacgttgcgt ttggtattac aatcgaacaa gcagtccgag
     3781 cattaaacga atctagataa ggagtgaacc attccaaatg gcgactgatg agatctcgga
     3841 aaatcaataa aagacgtttg taacggtaat ccgcgacaaa cgtctttttg tattcattac
     3901 atatatttgg aaattgtctc tctttatctc tagacctaaa caaaaacaca agttctaata
     3961 tcaaccatct cgttttttat gacaattttg ttagctgttt cgatgataat attccgttat
     4021 tatctgtata atatttaata
//
LOCUS       DACDCJ010000148         3937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDCJ010000148 DACDCJ010000000
VERSION     DACDCJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.152"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IV038_002833"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IV038_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IV038_002834"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IV038_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP8342980.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IV038_002835"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IV038_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IV038_002836"
     CDS             complement(2143..2373)
                     /locus_tag="IV038_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP8342981.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLRYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IV038_002837"
     CDS             complement(2603..3421)
                     /locus_tag="IV038_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP8342982.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..>3937
                     /locus_tag="IV038_002838"
     CDS             3582..>3937
                     /locus_tag="IV038_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8342983.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINS"
BASE COUNT         1279 a          670 c          674 g         1314 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatatctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttt
//
LOCUS       DACDCJ010000149         3914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDCJ010000149 DACDCJ010000000
VERSION     DACDCJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.153"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..292
                     /locus_tag="IV038_002839"
     CDS             <1..292
                     /locus_tag="IV038_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8342984.1"
                     /translation="LVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAAN
                     KNKVFFYPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            305..2071
                     /locus_tag="IV038_002840"
     CDS             305..2071
                     /locus_tag="IV038_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP8342985.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            2094..3902
                     /gene="uidA"
                     /locus_tag="IV038_002841"
     CDS             2094..3902
                     /gene="uidA"
                     /locus_tag="IV038_002841"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP8342986.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1423 a          473 c          797 g         1221 t
ORIGIN      
        1 attagttgat gaactgtgtt gtcaagataa aggaaaaaaa gaagatatta ttcgaagtgt
       61 aattgaaaat ggttgtgatt tggataagat attaatgatt ggtgattctc ctggtgatct
      121 agaagctgca aataaaaata aagttttctt ttatcctatt ttggtcaata aagaaaaaga
      181 atcatgggaa aacttaagaa ctgatgtttt atttaaatgt ttatcgggag attatgtaga
      241 tattgaacaa aaatatatag atactttttg gaaaaatctt actaataagt agaggagaaa
      301 gaaaatgact ataaaagtag atttacgaaa aaaaccatat aatttagatg aagatgctat
      361 ttcttggatt gaagagacga ttgattctat gactttggaa gaaaaaatag gtcaattgtt
      421 tgttaatatg ggatctagtc gtgataaaaa ttacttgagt aatatggtca atcagtacca
      481 tattggagct gtacgatata atccaggaac tgctgatgag gtatatgatc aaaactatat
      541 cttacaaaca gaaagtaaaa ttcctctatt aattgctgct aatacggaag caggtggtaa
      601 cggagcttgt acagatggta ctgaagttgg tgtagaagta aaaattggtg caacaaatga
      661 tgcgaaatat gcttatgaat tagggagagt ttctgggatt gaagcatcgg ctattggatg
      721 taactggagt tttgctccta ttgtagatat taatagaaat tggagaaatc ctatcatttc
      781 tagtcgatct tttggacaaa accctgataa agttttagaa cttagtttag cttatatgaa
      841 aggtattcag gagagtggaa ttttgcctgc agctaaacat ttccctggtg acggtattga
      901 cgaacgagat cagcatctat cttttagtat taatagttta ggtacggaag aatgggataa
      961 ttcttttgga aaagtatact caggcttaat agaagctgga ttaccttcaa ttatggcagg
     1021 tcatatccat atgccagctt attcaaaaca ttttaatcct gatctgaaag atgaagattt
     1081 acttcctgca actttatcaa aagaattaat cactgactta cttagaggaa aactcggatt
     1141 taatggatta gtagttactg atgctagtca tatgttagga atgactgggg caatgaaacg
     1201 atcagaatta ttgcccactt ctattgcagc tggatgtgat ctatttctat tttttaatga
     1261 tccagatgaa gactttaatt atatgatgga aggctatcgt aaaggaatta tcactgatga
     1321 tcggttatta gatgccttaa aaagaatttt agggttaaaa gctaaattgg gattacacaa
     1381 aaaagcaaaa gaacaaattt tattacctaa gcaagaagct ctggaaaaaa ttggtttaat
     1441 tgaaaataaa tcaattttta aagaggtggc tgaccaagca attacattag ttaaagataa
     1501 acaagatatt tggcctattg taccagaaaa atataaaagg attttactag tagatgtaca
     1561 tggggtaaaa ggtgggtttg gtgaagtagt tgctggaaat agacctcaag ccattgattt
     1621 attgaaacag attttgtcag caaaaggatt tgaagttgaa aaatacattt cacctgaaga
     1681 agaattgaaa ggattatcgc aagaagaaaa aattaaaaat atagctggaa tatatgctgc
     1741 taagagacca attaaaaatt taactaaaaa atatgacttg atcattaata tagctaatgt
     1801 taatccaggc acagttcaac gaattgtctg gccagcaagt aaaggaactc cggatatacc
     1861 gttttacatt catgaaattc caacaatttt tgtatctgta cagtgtccgt ttcatttggc
     1921 tgatgtacct caagtaaaga cttatattaa tacttatgat ggaaaacaag aaactttaga
     1981 aatattggtg gacaaactaa tgggtctttc tgaatttaag ggagtaagtc caatagatgc
     2041 ttattgtggt ttaaaggata catttattta atatttagat aggagtggtt tatatgttgt
     2101 atcccattat gacagagacg aggcaagtaa ttgacttaag cggtctctgg agatttaaat
     2161 tagatacaaa tgatgactta caggaagaac ttgctttaga accaataaat gaaaaagaga
     2221 gttatccgat ttcagttccc tcttcttata atgatttatt tgaaacagag aacattagaa
     2281 atcatgttgg atgggtgtgg tatgaaaaag aagtgactct tcctaaaaga ttattagaag
     2341 aaagattagt tcttcgtttt ggatctgcaa cacatgaagc aaaagttttt ttgaatggaa
     2401 agtttattac tcaacacaaa ggtgggttta caccatttga gtctgaaatt aatcggtttg
     2461 taaaagacgg gaataatcga attacagttg ctgtaaacaa tattattgac gatagtactt
     2521 tacctgtggg gattatgaag gaaacagtac aggaaaatgg ggatataaaa aaggaaaact
     2581 tagttaattt tgatttcttc aattatgcag gtattcatag acctgttaag ttatatacaa
     2641 ctccgaaaga ctatataagt gatattacta ttaatacaag aataactgat ttggatgcaa
     2701 gtgtagaatt caatatcgat acagaatgga ccggaaaaat cttaatagcg atatatgacg
     2761 aagaggctca acttgtagct gaagctgaag gaaagaaagg aatgattcag cttaagaatc
     2821 caattttttg ggaacctcta gacgcttatt tatatagtat gaaaattagt ttaattgatg
     2881 ataataaagt gatcgatacc tatacagaag aatttggtat tcgcagtgtt caggttacca
     2941 atggcaaatt tttgattaat aataaacctt tttattttaa aggatttggt aagcatgagg
     3001 attcgtatgt taatggacgt ggtttgaatg aggctgtaaa tattaaagat tttaatttaa
     3061 tgaaatggat aggtgctaat tcttttagaa catcacatta tccttactca gaagaaatta
     3121 tgcgattagc agatcgagaa ggtattgtag ttattgatga agtacctgca gtagggttac
     3181 atttaaattt catggccact ttattagatg ataatgtgga aaaacataat acgtggaaag
     3241 agatacgaac agaaaattct cataaaacgg tgatcaagga attgatagaa cgcgacaaga
     3301 accacccaag tgttgtaatg tggtctattg caaatgaacc agattcggat tccgaaggtg
     3361 ctaaagaata ttttgaacct ttagttgaat taactaaaga acttgatccg cagaaaagac
     3421 ctgtaacaat cgttacttat ctaaaatcaa cgccagatgt ttgtaaggta ggagatattg
     3481 tcgatgtttt atgtttaaat cgttattatg gttggtatgt ttcgggaggg gatctggcac
     3541 atgcaaaata tctgttgaaa aaagagctag atggatggat ggaaagatgt cctgatacgc
     3601 caataatgtt tacagaatat ggagcagata cagtagctgg atttcatgat acagtaccaa
     3661 caatgtttac agaagagtat caagtggctt attacgaagc aaatcacgaa gtaatagata
     3721 catgtgaaaa ttttgttggt gaacaagtgt ggaattttgc agattttgca acaagtcaag
     3781 ggattttaag agtgcaagga aataagaaag gaatctttac tagagataga aaacctaaaa
     3841 tgattgctta ctatctacag aaacgatggt catcaatacc caatttcagt tataaaaaat
     3901 aatttggatt tttt
//
LOCUS       DACDCJ010000150         3862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDCJ010000150 DACDCJ010000000
VERSION     DACDCJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.154"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IV038_002842"
     CDS             <1..391
                     /locus_tag="IV038_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP8342987.1"
                     /translation="RKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSYHVRNIDLMQS
                     TGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLEYQIF
                     NTELFEEYTYEVTGNIYENSELLEEQR"
     gene            388..945
                     /locus_tag="IV038_002843"
     CDS             388..945
                     /locus_tag="IV038_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP8342988.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            942..1238
                     /locus_tag="IV038_002844"
     CDS             942..1238
                     /locus_tag="IV038_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342989.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1315..1728
                     /locus_tag="IV038_002845"
     CDS             1315..1728
                     /locus_tag="IV038_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP8342990.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(2186..2461)
                     /locus_tag="IV038_002846"
     CDS             complement(2186..2461)
                     /locus_tag="IV038_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342991.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            2915..3121
                     /locus_tag="IV038_002847"
     CDS             2915..3121
                     /locus_tag="IV038_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8342992.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            3317..3484
                     /locus_tag="IV038_002848"
     CDS             3317..3484
                     /locus_tag="IV038_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8342993.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            3510..3854
                     /locus_tag="IV038_002849"
     CDS             3510..3854
                     /locus_tag="IV038_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP8342994.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         1336 a          599 c          828 g         1099 t
ORIGIN      
        1 acgaaagaac gtaatgagag atgtagccgt cttgcatttt actaaaaacg gcaaaacaaa
       61 ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc gaaacatcga
      121 cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gatatatttg aaggggatat
      181 agtattagtc agcgtgcgaa atggcttcga ttacttagat aataaagtct gtattgtcaa
      241 aaattcaata gattattccg gattagtttg tgccactgtt gatgaagact tagagtatca
      301 aatttttaac acagagctgt ttgaagaata cacgtacgaa gtcaccggaa atatatacga
      361 gaatagcgag ttattggagg aacagcgatg aataaacagg aagcaatcaa aaagttagaa
      421 agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag tataaattca
      481 ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac cagttattcc acaactcgtg
      541 gccggttggc ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat agcgtattta
      601 atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg agatggcata
      661 gtgacgcaag agcaaggaga agaattactc gcttgggcaa caagacaatc atatgaaaca
      721 ctattgagcc tatacaacgg ctacgaggtt gagaaagagc cgttatgggc aataaagaat
      781 gccgatggaa actatcttac taaatgtgct ttatggggaa aagatggagt aaattatagt
      841 tttgaatgca atccatctca tcgattgctt ttcactgata aagcaacagc ggatgctgca
      901 gcattgttgg tgaatggaac agtggaagag gtggtagaaa gatgaaacta aaagacggat
      961 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaacccta
     1021 aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa gcagaagagt
     1081 tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca atcattcgta
     1141 ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat ttagctgtag
     1201 cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat cgctccgact
     1261 gattcaataa atccaacaca tttattatat cacataaagg agcggtttga cttgatgcaa
     1321 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
     1381 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattata
     1441 acggatatgc cgaaggcacc gaagcatggc aataaggcag aagacgcgat tattcagatg
     1501 atggatatag aaacggagag agatgcgatt ctagcggctt tgatggctct tagtctgatt
     1561 agtcgtcaga tactctacta cagcttctgt gtgccagata gcttctcaaa ctacagaatt
     1621 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga agctctaata
     1681 gagtttgcag aagcatataa acacggaaaa ataattgctt ataaataatt tggcggtttt
     1741 ttggcggaat gatggcggtt tttagacatt taccagtgtt attatggtag tgtcgaaaga
     1801 ttaggaaaca ggatcgacaa aataaacgaa gggaggcaat ctccctcatc gttttaaact
     1861 tcttcttaga tagacagcaa ctaattatga agaaaaggat gtgaattcaa ctcctcctaa
     1921 attcttcatc ggttgctgtt tattttgatt agacagcagc actcacaaaa ctaaacatgt
     1981 tcttgtcgtc tttcaactgc tgctgtctat taatttatgt attggaggga agaaaaatga
     2041 aattatcagt agaaggcact ccagaagaaa tacaagaatt gctccaagct attggtagta
     2101 gcaaggagca aaaggtaagt gtttctaatt cagatattga cggtatttgc aattgtttaa
     2161 acagagaaaa aatatctaat ccttactaag agaaaatatg ttcgttaatg aatttccaat
     2221 tttcctctgt agctctacct tgataatttt tttgcagttc acagatgaat aaggaatcgt
     2281 tattatcaag tactgtattt aatttttcac acatttgatt tggattcaaa ttagttttaa
     2341 ctaaccaaaa agatttttgt agctttatat atgccccaaa ttcttttatt atttcaaata
     2401 ctttatcata cttttgccct gggttattaa gatcataact aatcatgtag ggtttataca
     2461 tgttgtcttg cacatccttg ttttatattt cagcggacca ctcgctgata aataaaatta
     2521 tacgcttagt atttattttc acaatattaa tttatcgtaa taacttttga gtaaatagaa
     2581 caaaaaacct gcactagttt ccgctagtac aggcagtgac tatatcggtt gataatctag
     2641 catatattaa aaataattgc aagaaaagaa atttatagtg gtttatacca aattatcaaa
     2701 gcaatgttat tttgttgctg tctattgttt tttaattatt cacacgataa ctaaaggtgg
     2761 gtgaagagaa atgattccat taataatttc aatttttgcg ctctgtctta atgtctatat
     2821 gattggattt aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg
     2881 tcactgtggc ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca
     2941 tgcaaggttc gattccttgc cagcgacatt aaatgcctat gacggttacg actaccgaaa
     3001 aaagatcgtt aagaagctat acggtgctac gtacggcaat gtagtaagtg tgctatctgt
     3061 acaccaccaa gcttcggtca ctgtggcgga agtagaagac gcagcggtaa atggcgagta
     3121 gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat cccactccag cgactttaag
     3181 caaccgaggc atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc
     3241 atcgtgcaag ttactattac atattagatc actctttgag tggtcttttt atttttgcac
     3301 aaaggaggaa acaacaatgt atagaccaca atacttagaa cagaagtatg aagtaatcac
     3361 tgttcataaa ggtaatggcg aaaaagtata tgagtataga agaccaataa agagcgatac
     3421 atataaacga aaggaaaaca atgaagttat tccattgtat ggcaaaagaa tagctaagca
     3481 ttaaataaga ttgcgaaagg agacggaaca tgaccgagga attctataga tggctattac
     3541 agttgacaag agaagatcgt ttggttaagt tctatcagtc tcctaaatgg cgcaggctta
     3601 gagagaaagc gatgaaacga gatcactatg aatgccaaga gtgtagaaga ctaggtaagt
     3661 atcatagagt agagaacgtt catcatataa aggaagtcaa ggatagacct gacttagctt
     3721 tagatttaga taatcttatt tgtttatgtg ttgaacatca taatgaagtt catggcagat
     3781 atcttacagc attagataaa caagagaaga agatagaaag ctttgctaac ttcgatgcaa
     3841 gtgaaaggtg gtaagtgcat ga
//
LOCUS       DACDCJ010000151         3764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDCJ010000151 DACDCJ010000000
VERSION     DACDCJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.155"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..305
                     /locus_tag="IV038_002850"
     CDS             <1..305
                     /locus_tag="IV038_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8342995.1"
                     /translation="MIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENE
                     IETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            278..955
                     /locus_tag="IV038_002851"
     CDS             278..955
                     /locus_tag="IV038_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP8342996.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            945..2087
                     /locus_tag="IV038_002852"
     CDS             945..2087
                     /locus_tag="IV038_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP8342997.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2100..3077
                     /locus_tag="IV038_002853"
     CDS             2100..3077
                     /locus_tag="IV038_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP8342998.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3077..>3764
                     /locus_tag="IV038_002854"
     CDS             3077..>3764
                     /locus_tag="IV038_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8342999.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPT"
BASE COUNT         1406 a          460 c          707 g         1191 t
ORIGIN      
        1 ttatgatcgg aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa
       61 taaatttagg aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc
      121 taaatgaaaa tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa
      181 gcttgaaaaa tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa
      241 cattgaatca aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt
      301 attaaaatat actagtgatc catacattat tattggggct ggcggacacg caaaagtcat
      361 tattgaaacc ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa
      421 taaaatgcaa gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag
      481 caaaaaaaaa atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat
      541 tataaaaaaa ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat
      601 agtggcaaaa gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa
      661 cccagatgct gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga
      721 ttgtagaatt aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca
      781 tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg
      841 atcttggtgt atgataggcg cagggagcac tattgttaag gatattccct cacattcctt
      901 agcttatggc aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt
      961 tattatcaag tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag
     1021 ataattggat agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat
     1081 atactggttc taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg
     1141 ccttaagact gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta
     1201 tagctacagt aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac
     1261 ctaatacatg gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa
     1321 acaaccaatt acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg
     1381 aacgaatttt agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat
     1441 cgttaggttc tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt
     1501 catttaacgg taataagatc atcacaacct caggtggagg agctctagtg tctagtgata
     1561 aaaaatatgt tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt
     1621 atcaacatag cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac
     1681 gtggacaaat ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat
     1741 ataaggaagc tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg
     1801 tgccaaatcg ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag
     1861 ttaacgtaat aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac
     1921 tacatttgca accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg
     1981 tgtcagaaaa gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata
     2041 gtgaacaaga atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta
     2101 tgaacatagg aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag
     2161 aacactttaa tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag
     2221 ttgaaaagga atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg
     2281 aaataaattc agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg
     2341 ctttaaattt agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg
     2401 aaataatcga agtagctaaa aagaaagata gattacttta tctatcttct acatttttga
     2461 agagaaaaga aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc
     2521 aataccatgt aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt
     2581 ttgtttcaaa caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga
     2641 ttatagatac atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc
     2701 ttgatattga ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg
     2761 gaactttaag tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg
     2821 aaaagatttt aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa
     2881 cttctagtat gacagatatt caattgtacg atgaggttat gcatgataat aattattcac
     2941 atactattat tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa
     3001 agaaaatgga accccaatat acttttgaaa aagataagta tattattaac ttaattaacg
     3061 acattgaggt aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag
     3121 acatctaaaa aatctaataa gctatggaaa aaagaattct attaattttg atataacagc
     3181 ttatagtagc agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga
     3241 tttggataca cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac
     3301 actaaatgta attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga
     3361 gagtattgat cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact
     3421 gaggtataaa aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc
     3481 tgtaagagct atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa
     3541 taattattct gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt
     3601 ggattatatt ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata
     3661 ttcgaatctt gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa
     3721 aattatagaa attcatttag attattttgg aagagttcct acaa
//
LOCUS       DACDCJ010000152         3684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDCJ010000152 DACDCJ010000000
VERSION     DACDCJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.156"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            54..506
                     /locus_tag="IV038_002855"
     CDS             54..506
                     /locus_tag="IV038_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343000.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IV038_002856"
     CDS             520..1209
                     /locus_tag="IV038_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343001.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..>3684
                     /locus_tag="IV038_002857"
     CDS             1334..>3684
                     /locus_tag="IV038_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP8343002.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1254 a          478 c          769 g         1183 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta atta
//
LOCUS       DACDCJ010000153         3680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDCJ010000153 DACDCJ010000000
VERSION     DACDCJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.157"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..276
                     /locus_tag="IV038_002858"
     CDS             <1..276
                     /locus_tag="IV038_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP8343003.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IV038_002859"
     CDS             419..640
                     /locus_tag="IV038_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8343004.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IV038_002860"
     CDS             656..1498
                     /locus_tag="IV038_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343005.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IV038_002861"
     CDS             1561..3363
                     /locus_tag="IV038_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP8343006.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3680)
                     /locus_tag="IV038_002862"
     CDS             complement(3422..>3680)
                     /locus_tag="IV038_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8343007.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1311 a          521 c          750 g         1098 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcctatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaatggatat agtaatgccc
//
LOCUS       DACDCJ010000154         3638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDCJ010000154 DACDCJ010000000
VERSION     DACDCJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.158"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..330
                     /locus_tag="IV038_002863"
     CDS             <1..330
                     /locus_tag="IV038_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8343008.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="IV038_002864"
     CDS             complement(844..1011)
                     /locus_tag="IV038_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8343009.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(1099..3039)
                     /gene="tet(S)"
                     /locus_tag="IV038_002865"
     CDS             complement(1099..3039)
                     /gene="tet(S)"
                     /locus_tag="IV038_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP8343010.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            complement(3040..3126)
                     /locus_tag="IV038_002866"
                     /pseudo
     CDS             complement(3040..3126)
                     /locus_tag="IV038_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
BASE COUNT         1169 a          606 c          563 g         1300 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatgtaagtt gattttatta aacatatgcc ttactttgtc
     1141 tattctatta tttggacgac gtggttggaa agcagactta atgttagtaa cctgataacc
     1201 ttttaactct gttaaacaga cactgcgtcc atttgtaaag aaagttaaac tgtttcgata
     1261 ctcttgaata caacgggctg gaatttcacc aatgagaatg acctcgttac cttttaactt
     1321 agtatttaaa atatttgcac aatatttgga agcatcatta tatgctctcg aaagatattc
     1381 ttgtggtaca taaatttcga agctaagata tggctctaat aactctgtac cactctttct
     1441 aaaagcctgc tctagtacaa taggcgcaag cattcggaaa tctgctggcg tactgacagg
     1501 gctgtaatat agaccatact taaaacagat cttacagtct gttaatttcc aaccgtacaa
     1561 tccttgttca cacccatagc gtataccttc cataactgca ttttgaaatg attgatttaa
     1621 ataacctaga gaaaccaggc tctcatactg aatgccacta cccaaaggaa gtggtgttac
     1681 agataaacca atagaagccc agaaaggatt tggaggtact tcgatatcaa tggtaaattc
     1741 agattttttt aatggtcttt ccatataaat gacagttggt tttcttgttt ctatctcaat
     1801 atgatatttt tcttgaatca gagtacaagt tacctccatt tggacctcac ctaaaaaaga
     1861 tagcacaatt tcgtgagtta ctgtatctac ataatattgt agaaggggat cactatcgga
     1921 tatttcaaaa agtgcatcta gtaacttttc tctttgtact gatttacatg gttcaattgt
     1981 tgtttgtaac ataggaagag gattctcaag aatttctcta tgtggtaatc tttttttatc
     2041 tccaagtacg ttatttagtt ttaaaagctc attttttaaa ataataatct caccaggctc
     2101 tgccttatct atctggcgta attctccatt tattgaagta tacatttctg taacttttat
     2161 tttttccttt tctgatatat tgactgagtc tcgcaaatgt agcgttccac tataaagacg
     2221 tacatagaca agccgttgac catcatctga atattctact ttaaaaacat ttccacaaag
     2281 tttatctgaa ttgagttgtg tgggtgaaaa taatttgcta gtaattacct ctataagttg
     2341 tttaattcca atattattct ttgcacttcc gtgataaaca ggatataagg agcaactttg
     2401 aattctttcg ttttcctcct tttcaagttc tgcaatattc aatgttttcc caatggtata
     2461 tttttctaat aaataatcat ttcccacaat tactgtctcc cattgttctg gttcagtata
     2521 atctattaca taaggtttca aatttagatt cacagtctgc ttaattatga tgtcgtcaga
     2581 aagtttgtcc ttaatatctt gataaacatc tggcaaattt attccatttt gatcaatttt
     2641 gttaataaaa aatattatag gtatgttcat ctttctaagt gcatggaata gtatacgagt
     2701 ttgtgactgt actccatctt ttgcagagat tagcaaaata gctccatcca aaacagataa
     2761 tgaacggtat acatctgcca aaaaatccat gtgtccagga gtatctacaa tatttacttt
     2821 aacattttcc cgttgaaaag atgttattgc ggtctgaata gtaatacctc tctggcgttc
     2881 caaaaacata gtatccgttt tcgttgtacc gctatctaca cttcctaact ctttaattgc
     2941 tccgctactg tatagtaagc tttctgtcaa agtagttttt cctgcatcaa catgtgctaa
     3001 gataccgata ttaataattt tcaattttat ttcctccatt caaaagctca aaaagcagaa
     3061 aaaatcccag tgataaatac tcttatcact gggattttaa tacaaaaata gctgtatact
     3121 acacactgat agccacagat gctttatgat aaaatgataa aagtattctt aaactgggca
     3181 caaaaaacca gtccttattt taaataagga tgcttaatta attattcccg ctatctaatt
     3241 gacagtttat ttaagaatac cttgccgcat ttttattatc tccttttgaa tgttatctta
     3301 attctatcat atgatagaat gcttgtaaag ccaaccatat tttttgaacg aaagggaaat
     3361 taatgagaaa ttaatggaag ttagtgtagt agttgaagat aactcttcag aatggctatc
     3421 aaaattcagc gatttaataa cgatttacaa gttcataagg gattctttat tcatccagct
     3481 attatattac ttttcaggtc aataggacaa ctgtccacaa cctttctaca aatatattac
     3541 gtataatttt tgaaatatat actgcttaac aaaatatttt gcagtggttc caatagtttt
     3601 gaattgttaa tattaagttt gacttggaaa atatcatt
//
LOCUS       DACDCJ010000155         3625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDCJ010000155 DACDCJ010000000
VERSION     DACDCJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.159"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            251..529
                     /locus_tag="IV038_002867"
     CDS             251..529
                     /locus_tag="IV038_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343011.1"
                     /translation="MDIIIRDINTKYINEIDKRCEELRTKTDSKWSRNDYLKLLIEND
                     FERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILITH"
     gene            547..969
                     /locus_tag="IV038_002868"
     CDS             547..969
                     /locus_tag="IV038_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343012.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            989..2581
                     /locus_tag="IV038_002869"
     CDS             989..2581
                     /locus_tag="IV038_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343013.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMENGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKVAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIEGVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2593..2928
                     /locus_tag="IV038_002870"
     CDS             2593..2928
                     /locus_tag="IV038_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343014.1"
                     /translation="MKQYLNQWKVIEGSLKKERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLVPYYTTWEMNEEGINNFPCKSI
                     KESMSFTEC"
     gene            2971..3324
                     /locus_tag="IV038_002871"
     CDS             2971..3324
                     /locus_tag="IV038_002871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343015.1"
                     /translation="MIENKLTEKQEKFIDKFYFVTEKYLFPKTKGKYFLKGSIGAILF
                     FIVEFFSVFKSFGGYQKQIQESKELQHYFQMLTHSPLYFVMWIALFLLAFIIGYIGLT
                     LAHILIDWVRGNLLK"
BASE COUNT         1438 a          547 c          708 g          932 t
ORIGIN      
        1 cgacaaagtt agaaggattt tttattgtct aaatacacaa ataaccttaa tttttaaaag
       61 ggcataagga aacgtgatat cactttctta cgaaaggtgc taggtaggct gtgcctacca
      121 cgtttctaag ggaaaaatct gatatcaaaa attgagtttc atggatagtc tatggatagc
      181 aaaaaaccat attatggata gcttatggat accatccata gactatccat aatgtaaaaa
      241 ggagaatcat atggatatca ttatcagaga tattaatacc aagtacatta atgaaattga
      301 taaacgttgt gaagagttaa ggacaaaaac agactcaaaa tggagcagaa atgactactt
      361 aaaattattg attgaaaacg actttgagcg tcctttaatg gactacaaga aagatcaatt
      421 tgatcgttta ttggaaaaat ttacagatgt tcagctgcat aatacaaaag ttttggaagc
      481 atacacgaac gaggtcaaga acttaattga aatattaatc acacattagg aaggagataa
      541 tgttctatgg ttaaagcgat agaaaagcat attacatttt atgaagcgca cgatgaagtt
      601 ttaaaacata ttatggaaac tcaacctacg gtaaaaaatt attctgaagc cattcgattt
      661 ttatgtatga attacgatcc agaaattgaa aaaaataata atatgacgaa cgcaaagttg
      721 aatgcaatgg gaaaggaaat ttctatttta accgaaatgg tttcagagtt tgcggatctt
      781 catttgaaag atacaggaat actaattggt acaaattcaa ctgtctatca agaagcaaaa
      841 gagcgtgtgg aagggaaaat aaaagcgaat caaacgaaaa agtattctcc taagatcaag
      901 aaaccaataa atgaatctgt tcatgccaga gtagacccgt ttgatccaac aaagttttta
      961 aaaagataaa aagtagaagg ggaacatgat gaataaatcg cctgcagtca ttctcacttg
     1021 tcaatttacc ttgcccaata aaaaatcttt ttcaacatac attgattata tgacaagaga
     1081 aaaagcgctg gaagaaaagg aacgacgcac gcccgacgaa gaacaggaac tgcaacgaat
     1141 caagaatgct ttagaggatt tttatattcc tgaaggagaa actttttcgg agagtaagac
     1201 ggataaagaa ttcagcgaaa cgtcgaatga agctcaatcg ttgatggaaa atggaatcaa
     1261 ctttgacaag ttagaggaac aagattttac caaatatctt tcttatatga cccgtcacta
     1321 tgcgttagaa caaaagaaag aactgacgca aagtgaacaa aaagaatacc aacgcttgaa
     1381 ccaagcaata aaaaaatatc aaacagagcc acacaaaaag gcttcaggaa accttcctgg
     1441 agttttttct atggcttcag acgaagtgaa aggaagtgat ctgaaggaga ttaagcaaat
     1501 cttccaaaga ggacaacaaa aaggctcaat catttatcaa gatgttgtct ctcatgataa
     1561 ttcgtatctt gaaaaaatag gattgtacga tcccaaaaca gatactttag atgaagaagg
     1621 attaaaagta gctggaagaa aaatgatgga gacattattc gagaaagagc aactgaacga
     1681 aacaggctac tggatggcaa cgattcaccg taataccaaa catatccata ttcattttgc
     1741 agttgttgaa aaagaaaata cgcgtaaacc ttacacggag gttgaaaatg gtgtgagtta
     1801 tatcgtaccg aagggtaaga gaagacaagc aacattagac gcaatgaaga ccaattatgt
     1861 acatgagtta gaacgttttt cttatgaaaa ggatcgaatg attggtatgg agaaacgaaa
     1921 tttattgacg cggaaaagtg atttgcgtaa caccttaaca aaagtggtaa aagagccagc
     1981 gcgttacgat cgacacgcaa tgaacttatt aaatgaagta tataaaaccc tacctgacaa
     2041 acgttcggaa tggaattacg gggatgaaaa acgaaccaaa ctttcaccat acacaagaga
     2101 gaaactcgat gagttaaccc aatatgtgtt aacaaaagaa cctgaatacc aagaatatat
     2161 acagatttca aaagcattga aagaagaagc aaaaagtctt tatggggaaa gcaagagaga
     2221 atcaaaggat gcagaaaaaa acgccctctt tgatttaaaa aagcgcacag gaaatgccat
     2281 cctaacagaa ctgaagagac aggatagtca aataaaacca ttaatagaag gtgtcaatca
     2341 gatgcaatct atttacaatg aacgatcctt tcgaacgctt aaacaattgc caacgaatga
     2401 atacaaatat agttacgaag aattcatgaa gcgcaggaaa caattccaac aaagagttgt
     2461 cacgcagcgg agtttgcgtc aattaaatcg aacgattaat gcccatgaag aaaaatacca
     2521 tgcaagtaaa gcatacgaag agcaacaacg aagaattcaa atggaacaag aaagacagta
     2581 ggaggaatag caatgaaaca atatttgaat caatggaaag tcatagaagg atcactaaaa
     2641 aaagaacgaa tcgaacaact tcccgattgt ttggaaaaag agcatttgtt tcagataaga
     2701 gaaatgttga gaaacgaaca gtttgatcca aatcaatttt tagttgttga gtacccagct
     2761 actggcgtgt actgttgtaa tcatgtaaat ggtgaaaaat attttatcat tcaagaatat
     2821 gagggaaaat tagttcctta ttataccacc tgggaaatga atgaagaggg gatcaataac
     2881 ttcccatgta aaagcattaa agaatctatg tcattcacgg aatgctaatt ggataaaaag
     2941 aagaggcttg aaatagatgg aggaaaagta atgattgaaa acaaattaac agaaaaacaa
     3001 gaaaaattta tagacaaatt ttattttgtc actgaaaaat atttgtttcc taagacaaaa
     3061 gggaaatact ttttaaaagg gagcattggg gcaattctct tttttattgt ggaattcttt
     3121 tcagtgttta aaagctttgg aggttaccaa aaacaaattc aagaatcaaa agagctgcaa
     3181 cactatttcc aaatgttaac tcacagtccg ctttattttg ttatgtggat cgcgctattt
     3241 ctactcgctt tcattatagg atatatcggc ttaaccttag cacatatctt aatcgactgg
     3301 gtcagaggga atctactaaa gtaaaagaaa attgttctac ttacggaatg ttaattgaat
     3361 aaaagaagta aagagtcctg aacataattt gtctaggact cttttttcag tgaattgttt
     3421 tccgccagtt cactgagtgg tgcggtttcc aattttatta tatcaaatgg aagtgagatt
     3481 ccaaagggaa gggaggaaga agaaatgact cattcaacta ttggtgtcgt tgttgaaaaa
     3541 agcagagaca atctcgtttt tgtgacagaa attcaaacag atagagcttt tgtagtcaca
     3601 gacaaagctg caaaagcgta tcaaa
//
LOCUS       DACDCJ010000156         3524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDCJ010000156 DACDCJ010000000
VERSION     DACDCJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.161"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(1965..2483)
                     /locus_tag="IV038_002872"
     CDS             complement(1965..2483)
                     /locus_tag="IV038_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP8343016.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1218 a          593 c          518 g         1195 t
ORIGIN      
        1 aaaaagcaga taaataggta tagatttaat aatttaaaat aaatggtata catgaatagg
       61 aaaggaaaca gaaggaggca atcttaattt tttaaattgt ctaaattata tagaaagaac
      121 aatctttcta tataattaga cataagaggg actacggttg atttgatatt aacaagcgta
      181 tatgaatgta tatacaaata aaaaagggga gggagaagtt aaagaaaatt cagagaagtg
      241 gggcactcag tagtattaac aatttaaaag gaatttctta aagaaaatgg gttgcatcct
      301 ggagatacaa tttttttaga taaagaaaaa attcaagcaa caattcaaaa aaatcttaac
      361 tgctgagatt gatgtagcaa tatctcaatc atcaacaaag tacgatcctg cctttcaaaa
      421 gcttattgat aaatgtttat tcattataaa aataaaagaa aaatttatgt cagctttttt
      481 catcaaagtg gtaatgataa ttcgtctatt aattgtattt tttaggaaat atggtataat
      541 tgttttaacg aaaaggtaat tcatgataca ccaaaaacca ctagctggcg ggctagtggt
      601 tttcttttac gttctattca gattaacggg taggcttact gtcttttttg gcaataattt
      661 tgcaatcaag ccatttacag atgtaatgcg cggatacttc tgctaagatt gcaaaaaaca
      721 tatcgaaaag gtaattcata atacaacctc ctccccttgc cataataggc cggggtagca
      781 gacagcataa atattatacc ataactgaaa atttcatttt agtacatttc aatatattga
      841 tgaaccagca caataaaaca tgccatgact aacagaaaaa aacttattaa accagatata
      901 acttgtaaac atccaactat atagaaaacg taaattttct atatagttgg attaagataa
      961 cgactattta ttttatcata tttttactat accctatact tttacttggt atatttatga
     1021 ataatcgcaa atttacccct atactactag tttgtaagtt agattaataa aaatactaaa
     1081 gttatttgtt cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca
     1141 cgaaagaata aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa
     1201 gtgagttagt gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc
     1261 ttgcagaaat cggtactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt
     1321 tgaacttcta ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt
     1381 caaatcccgt atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag
     1441 gccacatacc ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt
     1501 agcatattaa caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca
     1561 atttcaattc ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc
     1621 atgtgggttg ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa
     1681 aacatcccaa aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga
     1741 tatggcacca taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa
     1801 attgatacaa tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac
     1861 ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat
     1921 acaagaggtc tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc
     1981 ttccattttc ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata
     2041 aatttatata cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt
     2101 gcagtgaata gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta
     2161 cgattgagaa tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac
     2221 ttatataact ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat
     2281 ccatttcttt catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct
     2341 aaattttcag caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga
     2401 atagtttcaa attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct
     2461 tctttatttt tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa
     2521 ttcatctata atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca
     2581 ttgactgtct aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa
     2641 atgttgatcg tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg
     2701 actggtttgc atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac
     2761 taataaaaac taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt
     2821 cacaaaatcc attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat
     2881 tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg
     2941 acgatgacac gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac
     3001 tcgaagaaaa tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta
     3061 ttaaaggtca agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg
     3121 cacaatgcat ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg
     3181 taatgaaaca gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata
     3241 attatgttaa tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat
     3301 tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg
     3361 ttaagttggt tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg
     3421 gacagtacct aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct
     3481 gctcatttca acttatatgt tttagtgaac tacccccact tagc
//
LOCUS       DACDCJ010000157         3487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDCJ010000157 DACDCJ010000000
VERSION     DACDCJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.162"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(160..567)
                     /locus_tag="IV038_002873"
     CDS             complement(160..567)
                     /locus_tag="IV038_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343017.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(586..1404)
                     /locus_tag="IV038_002874"
     CDS             complement(586..1404)
                     /locus_tag="IV038_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8343018.1"
                     /translation="MDIFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(1630..1923)
                     /locus_tag="IV038_002875"
     CDS             complement(1630..1923)
                     /locus_tag="IV038_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP8343019.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFTSNQQRKVKKQID"
     gene            complement(2060..2239)
                     /locus_tag="IV038_002876"
     CDS             complement(2060..2239)
                     /locus_tag="IV038_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343020.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
     gene            complement(2236..2586)
                     /locus_tag="IV038_002877"
     CDS             complement(2236..2586)
                     /locus_tag="IV038_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343021.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMTTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPDDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(2579..>3487)
                     /locus_tag="IV038_002878"
     CDS             complement(2579..>3487)
                     /locus_tag="IV038_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAP8343022.1"
                     /translation="RRKKLAQMIQERIKEELGLIATVGVGDNPLLAKLALDNEAKHNE
                     GFIAEWTYKNVPEKVWNIPEMTDFWGIGSRMKKRLNQMGIMSIRDLANWNPYTIKSRL
                     GVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQV
                     AIRIRQHNCKTGCVHLDIGTSILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEG
                     QEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEKLDKIIDKIRQKYGFTSIVHASSM
                     LEGARSITRSTLVGGHAGGAGGLKND"
BASE COUNT         1013 a          649 c          519 g         1306 t
ORIGIN      
        1 aacttatact ttaaataata attttataat gttgatttaa tcgcaaattt acacgttgtt
       61 tattaagtta taccttaaat atttaatgta taacttatac tttaatattc atttatctag
      121 tataacttat actttaattg aataccgcat gaaaaaaaat tattttggta atttttcaaa
      181 ctcttcttgt atttcttctc ttatgtgttc ttcttttgct actcctagtc tatgctggat
      241 atatgagtct gctaatgtat taacaatttt ttcgaaagaa attctccctt tttcactttg
      301 taactccgtc atatagtctt gtaaaattga gagtttgcta tttaatgccg gagagatctt
      361 tttaggaact gataccttgt ttcttttatc aggaattgaa actggtcggc ctcttttttt
      421 caatgtaata tttcttgtgt tactcaatgc actattgcta aagttatatt caccgcttat
      481 tttcacatct tggcgtttgt tattgggtaa taatggctca aattcgccat tctccatcat
      541 tttaggaata tcaaaatctt taggcattac ttatgcaact ccttattatt tatttaaaaa
      601 ctcttgtcta gcatctagtt ctgccaaaat attgataaat acttggtgtg ctttataatc
      661 ccattgatca aaatttccat ttttgtattt tcttaaatgt ataccaattt tagaatacct
      721 tctcaatctt ttttgataat gaatgagatt tttggatacc actgttccgt atttttcaat
      781 tgcttcttct aggatttctt catcaaaatc atcatttggt tctaccatac aaggtacaat
      841 atctataatt tgcaaatcaa gatcgtatct atcgaccatg aatttttgat aatttacata
      901 tttgttgact ccctctaagg attcttcagc tgtttgaaat gaaattatcg aatagtctga
      961 agctgccata gcattatcgg aaaattcgct aatggttggt ggtacatcaa taaatatttc
     1021 ttgataattg ttctttattg gaagaagtaa ttttttcaag acagtaactt ggtctaattc
     1081 attttcatgt ttattagcaa aagcggagaa tgatcgaaaa ctagtgtcac atgccagaag
     1141 gtcaagattt tcatttacct taataatact acttgctaat tctccatttg cgacaccatt
     1201 tgttatagtg gttttgatat cggttatacc gaaagttctt gctatgactt gtgttaagtt
     1261 cgcttgaggg tctaaatcaa taataagagt ttttctattt cttatcattg cattgtcata
     1321 agcaagcatg ctgactatct tagttttacc tactcctcct ttaaaattgc caactgtaaa
     1381 tgtagtccct tgaaaaatat ccatgtaaat cctcccaatc tttaattgat aattaaagta
     1441 taacttatat atcggaaatg gtcaacatta aaagtataac ttaaataata atatctaaaa
     1501 acgtttggat atagcggttt ttaagaataa aagtataagt tataccatat atatttaggg
     1561 tataacttat acttttaata tttaaggtat aacttatacc ttaaatattg cttttcgaaa
     1621 atagctattc tagtctatct gtttctttac ttttcgctgc tgatttgaag tgaattgatc
     1681 atattcattt tctagtatta ataacttttc gtatacatcg gtcatattca tactagaagc
     1741 aagacttaga tcatatacaa atgaagcagg taatgatttt attctacgtt gtcgggttat
     1801 ccagcttgca accgttcctt gtgggtaggc atataactca caaaattgtt ctacagtgat
     1861 tcctaaattc ttgataatgt aaacattgat cgggtgcgag taaacaaatg tattgataga
     1921 catggtgcgt aagcctactt tcttttatta ttgcgtttgc aataatagtt taacacacgc
     1981 attattctag tatctactcc tatttgtaaa aaacaaaaaa ggattcatag agataactct
     2041 atagaatccg taaaatacct tatataaata acgggaaaat ataaaaccta aaattatgat
     2101 aacctgctgc agcttctaca tagaaagaga agcgctcaac accaacctca tttatcaaac
     2161 gttggccata accccaaatc tcttctgata gctgatcaaa ggttatatac tttagctcat
     2221 attgctggat aatgttcatt ctaatcgact ccatttatta tcctggtaaa attcgatatg
     2281 acgtatttcg tcgtagtgaa ttttgtcttt gccaacgtag atccccagct catcactacc
     2341 taatattttg ccaattatat cgtctggata gtatccttca gcatttcgtt cttctttttg
     2401 aatacatact aatctatttt ttatgactgc agcgttcaat acttccgaaa tctcttcagt
     2461 cgtcatttgt tcttttgcta agttttgatt ggctcgctgt tcattgtccg catcgatctc
     2521 ttccgtgtga tcggataaat agaatcctgc ccatttcaat cgtcctcgat cgttatagtc
     2581 aatcattttt aagcccccct gcgccgcctg catggccacc aaccaacgtg cttctagtaa
     2641 tactacgagc gccttctaac atcgaagaag catgtacaat tgacgtaaaa ccatacttct
     2701 gcctaatttt atctattatt ttgtctagtt tttcgtcatc aatctgtttt tgtggatcac
     2761 taaacaagtc cagctgaagc gaatcagtat aaactagctt gctgtaagtg attccgacgt
     2821 ggcggacttc ttgtccttca taatacttat caaacaagaa aagacaataa ttctgcaatt
     2881 cctttgtatt gtctgtgata gggattttca tctggtgtga gaaccctggt ctcgtttcta
     2941 agatcgatgt accaatgtct aaatggacac atcctgtttt acaattatgc tgtctgatac
     3001 gaatggctac ttgttccgcc atttctttta ccaccagctc gatttcattt cttctagtat
     3061 aatcacgtgg caacacttgt gaattaccat aggacttttc ttttgtcggt tctggtggga
     3121 ttgctatatc agttcgatca ataccatttg catgaaagta tagctgtaag ccaatcacac
     3181 ctaaacggct cttaatggtg tatggattcc agttagcaag atctctaatg ctcataatcc
     3241 ccatctgatt gagtcttttc ttcattcgag agccaatgcc ccaaaagtcc gtcatttcag
     3301 gaatattcca tactttttca ggaacgtttt tgtacgtcca ttccgcaata aatccttcat
     3361 tatgctttgc ttcattgtct aaggctagtt tagccaaaag gggattatcg ccgacgccta
     3421 ccgtagcaat caaacctaac tcttctttaa tacgttcttg gatcatttga gcgagcttct
     3481 tccttcg
//
LOCUS       DACDCJ010000158         3448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDCJ010000158 DACDCJ010000000
VERSION     DACDCJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.163"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            68..151
                     /locus_tag="IV038_002879"
     CDS             68..151
                     /locus_tag="IV038_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8343023.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="IV038_002880"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="IV038_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8343024.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="IV038_002881"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="IV038_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1606..2271
                     /locus_tag="IV038_002882"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="IV038_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="IV038_002883"
     CDS             2307..2552
                     /locus_tag="IV038_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8343025.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..>3448
                     /locus_tag="IV038_002884"
     CDS             2655..>3448
                     /locus_tag="IV038_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8343026.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLY"
BASE COUNT         1241 a          519 c          636 g         1052 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag gggtttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataa
//
LOCUS       DACDCJ010000159         3412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDCJ010000159 DACDCJ010000000
VERSION     DACDCJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.164"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..203
                     /locus_tag="IV038_002885"
     CDS             <1..203
                     /locus_tag="IV038_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP8343027.1"
                     /translation="PLNENSNGILRRNGLPKSMDFREVNQTFISSVSNQRNHIPRKSL
                     NYRTPIEIFLSYVQEAFYSSLI"
     gene            697..984
                     /locus_tag="IV038_002886"
     CDS             697..984
                     /locus_tag="IV038_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343028.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            1002..1424
                     /locus_tag="IV038_002887"
     CDS             1002..1424
                     /locus_tag="IV038_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343029.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            1444..3036
                     /locus_tag="IV038_002888"
     CDS             1444..3036
                     /locus_tag="IV038_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343030.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
BASE COUNT         1366 a          525 c          649 g          872 t
ORIGIN      
        1 gcccattaaa cgagaattct aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg
       61 attttagaga agtgaatcag acatttattt ccagtgtcag caatcaacgt aatcatattc
      121 caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat gtacaagaag
      181 cattttattc tagcttaatt tgacaaatca tatgaaaaaa gtatataaaa aatgtttatg
      241 gatcagctat atcaccctag gtatagctgt taaagagatg ggagaaaaca ttaaccacga
      301 ttctaacaca aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt
      361 tcaaccaaac aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt
      421 tcatatgaca aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa
      481 tacacaaata accttaattt ttaaagagca taaggaaacg tgggaacact ttttaaaaaa
      541 agtgttccaa taggctgtgc ctattacgtt tctctaaaac aaaagtgggt acaaaatatt
      601 cgatttatgg atagtgtatg gatagtaaaa cagctataca tggatagcat atggatagac
      661 tccatagact atccatactt ttttcggagg gtgaaaatgg aaattaaaga gattaaagta
      721 agaggtatta ataaaaaata tgttcaagaa attgatcata gatgcgtaga attaacagaa
      781 caaactggtc aaaaatggag tcgaaatgat tatctaaaac tattgattga aaacgacttt
      841 gaacgtcctt taatggacta caagaaagat caatttgatc gtttattgga aaaatttaca
      901 gatgttcagc tgcataatac aaaagttttg gaagcataca cgaacgaggt caagaactta
      961 attgaaatat taatcgcaca ttaggaagga gataatgttc tatggttaaa gcgatagaaa
     1021 agcatattac attttatgaa gcgcacgatg aagttttaaa acatattatg gaaactcagc
     1081 ctacggtaaa aaactattct gaagccattc gatttttatg tatgaattac gatccagaaa
     1141 ttgaaaaaaa taataatatg acgaacgcaa agttgaacgc aatgggaaag gaaatttcta
     1201 ttttaataga aatggtttca gagtttgcgg atcttcattt gaaagataca ggaatattaa
     1261 ttggtacaaa ttcaactgtc tatcaagaag caaaagagcg tgtggaaggg aaaataaaag
     1321 cgaatcaaac gaagaagtat tctcctaaaa tcaagaaatc aataaatgaa tctgttcatg
     1381 ccagagtaga cccgtttgat ccaacaaagt ttttaaaaag ataaaaagta gaaggggaat
     1441 atgatgaata aatcgcctgc agtcattctc acttgtcaat ttaccttgcc caataaaaaa
     1501 tctttttcaa catacattga ttatatgaca agagaaaaag cgctggaaga aatggaacga
     1561 cgtacgcctg aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat
     1621 attcctgaag gagaaacttt ttcggagagt aagacggata aagaattcag cgaaacgtcg
     1681 aatgaagctc aatctttgat gggaaatgga atcaactttg acaagttaga ggaacaagat
     1741 tttaccaaat atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg
     1801 acgcaaagtg aacaaaaaga ataccaacgc ttgaaccaag caataaaaaa atatcaaaca
     1861 gagccacaca aaaaagcttc aggaaacctt cctggagttt tttctatggc ttcagacgaa
     1921 gtgaaaggaa gtgatctgaa ggagattaag caaatcttcc aaagaggaca acaaaaaggc
     1981 tcaatcattt atcaagatgt tgtctctcat gataattcgt atcttgaaaa aataggattg
     2041 tacgatccca aaacagatac tttagatgaa gaaggattaa aagcagctgg aagaaaaatg
     2101 atggagacat tattcgagaa agagcaactg aacgaaacag gctactggat ggcaacgatt
     2161 caccgtaata ccaaacatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt
     2221 aaaccttaca cggaggttga aaatggtgtg agttatatcg taccgaaagg taagagaaga
     2281 caagcaacat tagacgcaat gaagaccaat tatgtacatg agttagaacg tttttcttat
     2341 gaaaaggatc gaatgattgg tatggagaaa cgaaatttat tgacgcggaa aagtgatttg
     2401 cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt acgatcgaca cgcaatgaac
     2461 ttattaaatg aagtatataa aaccctacct gacaaacgtt cggaatggca ttatggggat
     2521 gaaaaacgaa ccaaactttc accatacaca agagagaaac tcgatgagtt aaccaaatat
     2581 gttttaacaa aagaacctga atatcaagaa tatatacaga tttcaaaagc attgaaagaa
     2641 gaagcaaaaa gtctttatgg ggaaagcaaa agagaatcaa aggatgcaga aaaaaatgct
     2701 ctctttgatt tgaaaaagcg cacaggaaat gccattctaa cagaactgaa gaaacagaat
     2761 agtcaaataa aacctttaat agaaagtatg gatcagatgc aatctattta caatgaacga
     2821 tcctttcgaa cgcttaaaca gttaccaacg aatgaataca aatatagtta cgaagaattc
     2881 atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc aacggagttt gcgtcaatta
     2941 aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa gtaaagccta cgaagagcaa
     3001 caacgaagaa tccaaataga acaagaaaga cagtaaagac agtaaagtta gcgccttatt
     3061 atactacttg ggaaatgaat gaagagggga tcaataactt cccgtgtaaa agcattgaag
     3121 agtctatttc actcacagaa tgttagttgt taagtgaata aaagaagtaa aagagtcctg
     3181 aacgtaattg tccaggactc tttttttcaa tgaattgttt tccgccaatt caatgagtgg
     3241 tgcgatttgc gactacatta tatcaaaaga aagagaaggg aggaagaaga aatggcttat
     3301 tcaacgattg gagttgttgt tgaaaaaagc agagacaatc tcgtttttgt gacagaaatt
     3361 caaacaggca gagcttttgt cgtcacagac aaagcggcaa aagcgtatca aa
//
LOCUS       DACDCJ010000160         3375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDCJ010000160 DACDCJ010000000
VERSION     DACDCJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.165"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1073..1399
                     /locus_tag="IV038_002889"
     CDS             1073..1399
                     /locus_tag="IV038_002889"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8343031.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1407..2315
                     /locus_tag="IV038_002890"
     CDS             1407..2315
                     /locus_tag="IV038_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8343032.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            2944..3195
                     /locus_tag="IV038_002891"
     CDS             2944..3195
                     /locus_tag="IV038_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP8343033.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
BASE COUNT         1058 a          674 c          589 g         1054 t
ORIGIN      
        1 ctcctcttta tggtcttgtc cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa
       61 agcatttcta ggttttggac aataaggaat gacttttttc ttcatataaa tacctcattt
      121 aactatttga tatcatcaca ttatcaaaaa aaataggttt tttattaaaa ctatcttgac
      181 ctttcgggag atttcactgt gatttatttt tattcccata cttttttcgt tggtcacttt
      241 cgtataccta atctaagcga tcatcggcga cggtgctttt acaacgaatg gcaggacaaa
      301 acaaggggcg gaaaaacttt tttgcctatt tcataaaaaa ctttttccta cagcaatccc
      361 ttgttttgtc ctaccacacg ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg
      421 aaaatgacct ccaaaaacaa aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc
      481 ccaaaatgtc gtccgcctcg gagacaactt gctgctgaaa atggttggtt aattcattta
      541 agtattaaat aagataaatc atcaaattaa tcggaaaaat agcatgattg gccttatcaa
      601 aaataaactg taaaaactta tatttctatg agtggtggaa caatgctaat accaaattaa
      661 tttatcgatt acccacctgc acaaaccaaa gatagcatta ttaggaaacg aatcaatcag
      721 cgaatccagc attataatga ggatactaaa aataaagttt tcataattat aaatccgcct
      781 tcctacgaaa ggcaccaatg aggtgatgaa aaccttagag cgctttcttt ctttttttat
      841 tccgtgttac cctttccttg caggtccctg gtacactaca gaacccgagg aggtgagtgg
      901 cggggctgta tagcggcaac cccgcataat tatatggtgt cggtcatccg ttaaggcatc
      961 aatgaatggc gcaaacccgt agcccgtaaa cttatctctt ccgaagttga ctcgatttcg
     1021 ccggatgacc agtccctacc aaacctgtac tattttttta caggaggttg ctatgttcaa
     1081 aatctttcgt aaaaattgtt gtggcttgga tgtccacaaa acgtggatct atgcgtgtat
     1141 cggaatcaca gatgcgaatg gtcgtacagt gtataagcaa gctcgcttct cttcctttac
     1201 aaaaggtctc aatgaattag ctgaatggct tgctaaatac aactgtaagg atgtgtgttt
     1261 ggaatcttcc ggtaaatttt ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt
     1321 cacactggct catccgaagt acaccaaacc ccagaaagga aataaaacag atcgcaaaga
     1381 tgcaaaaatg gatttgtgat ctctttatgt gcgacatgat caaacccagt tttattcctg
     1441 gtcttgaaat ccgccagctt cgtgatctaa tgcgttatcg gacgaaactg accaacatga
     1501 aaacaagtga aaagaatcgt gctttaaact gcttaaccgt ttctaatctg aaactagatg
     1561 atgtttttag tgatgtgttc ggaaaatcat ccacttctat catccaatac ttgttagaac
     1621 atcccggtga atcatttgat gttcgaccat ttatccatgc ccgttgtaaa actcccgttg
     1681 aagaaatcca agcggcagtt gagggggcga tttctgtacc tcaagcgatt aagcttcgcc
     1741 aatgcctcaa ccatattaac gagattgaga agcatcgaga agagatcaat ctagagattc
     1801 aagctcttgc agaacccttc tcccctgttt tggaactact tcgtaccata ccgggcttag
     1861 acactcagcc aataacagcg attgcgattc tttcagaaat aggaccggat atgtccgtgt
     1921 ttccaacttc gaaacacttt atttcttggg ctggatgctg tccaagaaac gatcgcagtg
     1981 cccgacgaac aaaatcaaca cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg
     2041 tacagattgc caatgctctt ctcagatcca aagaacatcc agaatttaaa aatcgctatc
     2101 gtagaatcaa agcgaatcgt ggacacaaga aagctatcat tgctatttgt aagatgctcc
     2161 tgaccgctat ctggaatgtc ttatcaaagg tggaacccta ttctgctaaa ggatatctgg
     2221 agccaaaacc tgcgaaagaa gagaaagttc tgacgcaatc ccaagctctg gaccttttaa
     2281 gaaaacgcgg atataccatt atagatagtg cttaaaccaa agttacgttg attcttccta
     2341 aatagactcg ttgtcttcca tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc
     2401 gttcttcagg tatcttcaaa tagatttatt ttcaaactta tttcctttct tcttttactc
     2461 tcctatagct tagcatttaa gtgggcactc gtgtcacctt gctagatagc tatgaatgcc
     2521 gtaataagcc tcctagtata taggcagact gccaattata actgattcaa tccacattat
     2581 gataaaatcc accttttcgt ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg
     2641 agtcttttca ttcagtgctt tgtaaatact caaaattatt ccttttcact ttaaaaaaaa
     2701 gataattagt acaaaatcag aaatgaggaa agtattagga tggactagaa aggagttgtt
     2761 gaatttgttg caacagattg gtcatttatc aattaaaaat taggtgaagt tttattcatc
     2821 actgcagctg ctgtttccca gagaataaaa cgtctagaag aaactggtca gttactgtat
     2881 tatcaagcag caattgacta cgaacaagct tgattactaa ccaaagcgtt tatccacctg
     2941 ttgattaaat caaatcaaaa atcaggtttt tatacgttca taaaagcaat ctccagtgta
     3001 ctcgaatgtg actgtatcat aggtccttat tcgatgttaa tcaaaatggg gtttccttct
     3061 accgaatcat tggacagttt tatcaatgat ttatcaaagt ttggacgaac caacacccaa
     3121 atcgttttct cgactcttgt ttccttaatg gtttatcagt tctggaatgt ttatcatgcg
     3181 cttttattaa aatagaaaag agcttttttc atgcgacaaa gcagtgatca tttattggca
     3241 catgcaatct ttgtggatcc attttttgaa aatacgatat ttagttttga cccaaaaatc
     3301 tttaaatacg tatttgataa tttatcttaa caaccagata attttattca gcaccgtatt
     3361 atctatattg aattc
//
LOCUS       DACDCJ010000161         3287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDCJ010000161 DACDCJ010000000
VERSION     DACDCJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.166"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(200..949)
                     /locus_tag="IV038_002892"
     CDS             complement(200..949)
                     /locus_tag="IV038_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8343034.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1135..1839
                     /locus_tag="IV038_002893"
     CDS             1135..1839
                     /locus_tag="IV038_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP8343035.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            1879..2436
                     /locus_tag="IV038_002894"
     CDS             1879..2436
                     /locus_tag="IV038_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP8343036.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2444..2848
                     /locus_tag="IV038_002895"
                     /pseudo
     CDS             2444..2848
                     /locus_tag="IV038_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
     gene            complement(2901..>3287)
                     /locus_tag="IV038_002896"
     CDS             complement(2901..>3287)
                     /locus_tag="IV038_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8343037.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         1085 a          484 c          659 g         1059 t
ORIGIN      
        1 taataaaaag aaataacgta gtatttcaaa agacaaagaa gtgggatgat gattgctata
       61 gtatgttata cgtggtgcta ggtaagtgag aataaacaaa aaaggaaaca cgcaataaag
      121 tttatttcaa aactttattg cgtgtttctt ttttagattt tttatatctg ttgctttagt
      181 agttaccaat agttttcgct taatcaatca aaacgatttt ttgtttattg tattctttac
      241 gaaccttttc aggaatttct tctgaaataa gataggtaag gtcttcaatt ggaccgacga
      301 tatgattact gatggtgccg aatttatcat tagttatcat cccaagtact tcagtagaaa
      361 tacgtatcat ctcacgttta atgtcggatt cttgcatagt tggcacgctc gttccaacat
      421 ctagatctat gttataaata cccataatat aaaggtcggc tcgaatgttt tgtagttgct
      481 tataagcttg taccccaata ttgatcatag attgtttata aaaagtaccg ccaatattga
      541 taatttccaa attaggatgc tgtgctaatg ctatggcaat cggcgggcta ttggtaatga
      601 ttgttgcacg aaaatcttgt gggatttctt ttgctaaagc caagttagtt gtacttccat
      661 caatgatgat gacagattct tcttttaaaa aagggagggc tttatgagct aatgcagttt
      721 tttgttcgct agctacagtt tctcggtagt taaaattggt aattggcgga ccaacacgta
      781 aagcgccgct gtgtactctt tttaagagac cttggctatc caaatctttc aaatctcgtc
      841 gaatcgtgtc ttcggataca ttaaaacgtt gacttaaatc actagcgata actttttgtt
      901 ctgcgtttaa taaatctaga attttttgat gacgttctaa tttcaacatg ttgcgtgctc
      961 cttcttattt attcttgttt atcatgtcat ttatgcgttt ttatgtcaat aatttccgtt
     1021 tttaatacac taaaaaggca taaacaagca aaaacaaaat atttttatgc agttttatgt
     1081 tgactagtat ttgttaagcg gttacaatga gggggtaatt aaaggaggga atgtatggag
     1141 aaaaaaatta ttttgtcgcc ttcacttatg tgtggggatc tagcaaattt ggaaggaagc
     1201 atccaagaac tagaaagtat tgggatggat tccttgcata ttgacgtgat tgacggttct
     1261 tttagtccca gtatgccttt agggatcgaa acaatcaaaa gaattagaga aatcacttca
     1321 atgaattttg atgtacacat catggctaag gataacgata acgaattttt tattggagaa
     1381 atgttgaaaa ttggcgtaga gagcattact tttcattatg aaacaagcct ccatattgat
     1441 cgatatatcc agctcatcaa gaattctgga acaaaagtag ggatagcttt gaatccagca
     1501 actccgttaa atgtattaca atatatttta cccgagattg ataggatttg tttgatgctg
     1561 attaatccag gttttgcaac aaacaagtca gaaaaacaag tttcttacgc gctaaaaaaa
     1621 attacagatc tttgccagtt agtaaatgag aaagagttag cagtagatat ccaagtggat
     1681 ggacgggtat cgttagaaac gatcccggga ttattagatg caggcgcaac aaatctggta
     1741 ttaggaagta caagtttgtt tcttaaagga aatacattag cagaaaatca aaaactactt
     1801 tatcaagcga tgaagcaatc agttaggaaa aatcaataga tgtagtggtc ctgaataata
     1861 gaaatggagg ggaaatagtt gagttataag gcggcgaaac aaaatattgt aagagaaatt
     1921 actgaaacat tagaccgtat ttcttcaaaa gatgtggaac aattgataaa gaaaattgaa
     1981 aaagcggaac gagttttttt cgtgggggtt ggcagagtac tgttagcgct agaaggaatg
     2041 gcaaaacgct tagggcatct aggaattcag acagtagtcg taggacaaat cactgaaccg
     2101 gcaattacag ataaagattt attgattgtt ggttctggta gtggggaaag tgctttccca
     2161 ctaactattg caaaaaaagc aaaggaattt gggtcccaag ttgcccatat aggatcaaac
     2221 ccagacagca caatgaaaga atattcagat ctttttattc gtatacccgt acaaacaaaa
     2281 ttagcgttac cagaggaaat ttcttctata cagccaatga ctagcctgtt tgaacaaagt
     2341 gttcttttgt taggagatag tattgcttta gtgatgatag aagaaaaagc aattgatatg
     2401 tcaaagttgt ggcaatacca tgctaactta gagtaggtga aaaatgaaag cggtacattt
     2461 tggagcaggg aacatcggca gagggcttat tggacaaatt ctacatcaga atggattcga
     2521 tctatgtttt attgatacga atcctgaact gatccatcaa ttaaatcaag ataagggtta
     2581 tactatcgaa attattgatg aaatgaacag gagtgatttc attgatcatg ttgtggcact
     2641 gaatagttta tccgagctag aagaggtgat tacagcaaca aaaaatgcag atatcttaac
     2701 aacatctgtt ggcgcaataa atttagtaaa aattgcaccg atcattgcgc aagccttaag
     2761 taagcgattt gaacaaaagc atagcatcaa tatcgtagct aatgaaaatg tgataaatgc
     2821 aagtgatgtg ttgaaaaaag aaatttatgg gagcgtcaat aattttgtgt aaataaattg
     2881 tcctcctgca aaataattag ttactcagta aacattgaaa ctaatgtatc ggttacctgt
     2941 tgaaaacctt tatggcttct gtttagaaat ttttgattgt atgtatcaaa aatgctgact
     3001 agaaagcgtt ctagtgattc ttcattttga aactgctctt ttctacggct gtatctttta
     3061 atttgcttat tgaaagactc gattagattg gttgagtaaa tggttctacg aatgctaggt
     3121 ggaaaatcat aaaaagttaa taagtcttgg ttttctatga gtgactgcgt cactttagga
     3181 tagtttttct tccatttctc aatcatgccg gataagaagg tattcgcttc ttcttttgag
     3241 ttagcttgat aaacagcctt aaagtcatca cagatttctt ttcggtc
//
LOCUS       DACDCJ010000162         3265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDCJ010000162 DACDCJ010000000
VERSION     DACDCJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.167"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IV038_002897"
     CDS             complement(<1..2232)
                     /locus_tag="IV038_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343038.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3265)
                     /locus_tag="IV038_002898"
                     /pseudo
     CDS             complement(2904..3265)
                     /locus_tag="IV038_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          896 a          728 c          535 g         1106 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aacta
//
LOCUS       DACDCJ010000163         3222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDCJ010000163 DACDCJ010000000
VERSION     DACDCJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.168"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            67..483
                     /locus_tag="IV038_002899"
     CDS             67..483
                     /locus_tag="IV038_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP8343039.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            503..2047
                     /locus_tag="IV038_002900"
     CDS             503..2047
                     /locus_tag="IV038_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8343040.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            2190..2414
                     /locus_tag="IV038_002901"
     CDS             2190..2414
                     /locus_tag="IV038_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8343041.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            2429..>3222
                     /locus_tag="IV038_002902"
     CDS             2429..>3222
                     /locus_tag="IV038_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8343042.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLY"
BASE COUNT         1134 a          526 c          714 g          848 t
ORIGIN      
        1 tccagggatg agctgacatt caatttgaaa tgcggtcttt ccctgaaaga aaaggtggtg
       61 agataaatgg catatattcc atatggatac aaaattcaag atggagtggt tactgtcgat
      121 gaaaaggcag caggtcaagt aaaggtattc tttgagaaat acatatcagg actatccctt
      181 acagtggctg gcgaacaggc aggtattgaa aagacacatt ctgtgatggg gcgcattttg
      241 aaaaacgtca actaccttgg aaatgatacg tatccagcaa ttattgataa agagatattt
      301 gataaagctg aagaagttag agataaacgt gcaaaggatt taggacgagt ggtagagctt
      361 gccgctttca cctctccccc tcccaaagaa cgattcaaaa tgaaaaaggc agataataag
      421 atgccagtgg atccttttga acgagcagaa tacttataca gtctgataga aagcgaggaa
      481 taaagtgaca gagaaaaata taatggttat tcctgctcgt aaaagagtag gaagtacagc
      541 cgcaaaagaa aagataaaga aacttcgtgt tgctgcctat tgccgtgttt ctacagaaac
      601 agaagaacaa aattctagct atgaggttca ggtctcacac tatacagagt ttataaagaa
      661 aaataatgaa tgggagtttg ctggcatctt tgcagatgat ggtatctctg gtacgaacac
      721 taaaaagcgt gacgaattta atcgcatgat tgcagagtgt atggacggta acatcgacat
      781 ggttattaca aaatccatca gccgatttgc gcgtaacacc ctagactgcc ttcaatacat
      841 tagacagctc aaggataaga acatatcagt ttattttgag aaagagaaca tcaacaccac
      901 ggatgccaaa ggtgaggttt tgctgactat tatggcatct ttggcacagc aggaaagcca
      961 gagcctttca caaaacgtta agcttgggct acagtaccga taccaacaag gaaaggtgca
     1021 ggtcaaccat aagcgattta tgggctacac gaaagatgaa gatggaaatt taactattgt
     1081 ccccgaagaa gctgagatta tcaaacgcat ctaccgagaa taccttgagg gtcagagtct
     1141 agtgggcatt ggtcgagccc ttgaaaagga tggtatttta acagcagcgg gaaaaccaag
     1201 atggcgacca gaatcagtta agaagatact tcaaaacgaa aaatacatcg gagatgccct
     1261 tctgcaaaag actgtcacag tagattttct gaccaagaaa cgagtgaaaa atgaagggca
     1321 tgttccccaa tattatgttg aaaatagcca tgaagcgatt attcctaaag agttattttt
     1381 gcaggcgcaa gaagaaattc atcggagaag caatatctac acaggagaag gcaagaacaa
     1441 aagaatttac agtagcaagt acgctttaag tgccatcacc ttctgtggag attgtggcga
     1501 tatttataga cgggtctatt ggaatatcca cggaagaaaa gaatttgtct ggcgatgcgt
     1561 gactagaatc gagcaaggtc ctgaagtttg taagaaccga accgtaaaag aagatgaact
     1621 ttatggtgca gtaatgactg cgattaataa gctacttgca ggtgggaaca atatgataaa
     1681 gacactggaa gagaatattc atgctgtgat tggcgaaacc acagaatacc aaatttcaga
     1741 gattaacaac tcactagagg aaaaacaaaa agaactaatc aagctggcga acaagggtca
     1801 agactatgac cacttagcag atgagattga tgagctgaga gacaagcgac agatcctttt
     1861 agtagaagat gcctccctca gtggcgagaa cgagcgaatc aatgagctga ttgaatttat
     1921 ccgcaagaac aaattccgca ccttagaata tgatgataag cttgtaagga agataatcca
     1981 gagcgttaca gtctatgaag gccacttcgt catagccttc aaatctggca tcgaaatgga
     2041 aatatgaaaa ccagaagtaa atagcccatg actctacagt agagttgtgg gttttctttt
     2101 gcttgctgat tataataata ttttaataac tattgtgttt atcaaccaaa tggtttataa
     2161 tattcttagc gtaaacttgg aggtgctgta tgactacggc agaaatgatt aaagaactgt
     2221 gtgagcaaat gaatataagt gtttccgaac ttgctagacg tattggccag actccacaga
     2281 atttcaataa aaaattacaa cgagaaacag taaccttgga tgagttgaag gccattgctg
     2341 atgtgctagg tgtcaagttt gtgcaggcat ttattttacc agatggagaa gaaataaaaa
     2401 tatctaacga ataaaggagg atgtttatat ggtaggtaat gttattcaaa tagtaacaga
     2461 gaaattatca tctttgcctt ttatagaagg catcgtatta gggggctcac gtgcaagagg
     2521 cacccataca gaaaattctg atatagatat cggaatctat tacaattcag actcatttga
     2581 cctgacagcg attaatcaaa ttgctacaga attggatgat aaaaatagaa acaaccttgt
     2641 tgtgcctccc ggagcgtggg gtgattggat taatggcggc ggatggttat ttataaatgg
     2701 gtatcatgtt gacttgattt tacgtgatat aaaacgggta gaacaaataa tcaaagatac
     2761 ggagcaagga attgttactg ccaattatca gaccgggcat ccccatggtt atataagtgc
     2821 aatgtatcgt ggagaattag cgattagtaa aatactatat gctaagaacg aaagcttatg
     2881 cgaattaaaa aagcaggcag aaatttaccc tactgctcta aagaaaagct tgatgaactt
     2941 ttttatattt gaagctgaat tctctttaat gtttgtaaaa gcaaatgcgg gggtagagga
     3001 taaatattat attgcaggcc atgtttttcg tattatttca tgcttaaatc aagtactatt
     3061 tgcatgtaat aatgcttatt gcattaacga aaagaaagct ataaaactgc ttgaaacttt
     3121 tgaatataaa cctgaaaaat atgccgaaag agttaatcat atttttgaag tactcggtct
     3181 ttctcttttt gaatgctacg atatgaccga gaaactttat aa
//
LOCUS       DACDCJ010000164         2989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDCJ010000164 DACDCJ010000000
VERSION     DACDCJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.171"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            29..706
                     /locus_tag="IV038_002903"
     CDS             29..706
                     /locus_tag="IV038_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP8343043.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(843..1259)
                     /locus_tag="IV038_002904"
     CDS             complement(843..1259)
                     /locus_tag="IV038_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343044.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1262..1546)
                     /locus_tag="IV038_002905"
     CDS             complement(1262..1546)
                     /locus_tag="IV038_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343045.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(2246..2341)
                     /locus_tag="IV038_002906"
     CDS             complement(2246..2341)
                     /locus_tag="IV038_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP8343046.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          952 a          474 c          565 g          998 t
ORIGIN      
        1 ggaattaaca gtcaaatatc aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc
       61 taagcagatg gatttatttt ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa
      121 agttagattt tctttcgagg aattaaaaga gttaagtgat tataagatga ctgctacaaa
      181 agcctttgtc ggtgatttag aaaagctcta taaagatatg cttaatctta cttaccgtac
      241 tgaagatgag gattctattc gctactttgt tttgtttact ggttttgaaa tcaataaaac
      301 aaaaaagttt gttgaagtca gtgttaatcc ggacctagaa tatattatta atggtttaac
      361 agctgaattt agtagatttg aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa
      421 aactctattc cgactgctta tgcagtaccg ttctactggt tattatgtag ttcgtattga
      481 agagtttagg agcttaatgg atatcccaga atactatcaa atgggtaaca ttgatcaaaa
      541 agtattaaat cctgcattac gagaactaaa aaaatacttc aatgatctta aagtcacaaa
      601 aataaaagca aaaaagggca ataaaatcgc caaattagaa tttacttttt caagcctaac
      661 gtcagatttg ccaaagattt ctctgcgtaa ctggttagaa gaataaataa aaaagaactc
      721 gcccccctta cattaagcaa gtagacaagt cccttacaac cgactaagat tgctagtaca
      781 ttctagcata ttgtatgggg cttctaaata aaaaacagat aggaataact cctatctgtt
      841 ttttatcgtt ttagaaaatt tgttgggtcg aaatttctag aagtgactgt ttgactggtt
      901 tctttttgat gattctttgg tcgatttcga tttgatggat ttagtttagc ttcgattttt
      961 tctttcactt cttgatatac tttagaattt gtgccaataa gaacgcctgt atcttttaga
     1021 tataaatcag caaattctgc gatgatttca gtcatgatgg aaatttcttt tcccatagca
     1081 tttagttttg cctgattgct ggtagtggtc gtgttggtat cgggttcata actcatacat
     1141 aaaaagcgaa tggcttccga gtagttattg atatctggat ttttttcgat gatggaatct
     1201 aaaacgtcaa catgactagg gtacaaagta acatgttttt ggacagaagt ttttctcatg
     1261 gttatatcat tcctccgata agcatttcaa tgatttgatt atttgtgtag acatactcgc
     1321 ttaatatttg agtattttct gtttggatag caataaattt atcgagcaat aaatcgaatt
     1381 ggtctttttt gtaagtcgta agttgacggt caaaatcact ttcgattaaa acttttagat
     1441 atttatttcg actccatttt tctcctgttt tattcgtgag ttcttggcaa cgtttgtcta
     1501 ttgttgcaac aaactttgga tcaatattcc gaactaaaat gtccataaaa aatctccttt
     1561 tgcaattatg tataatttat gtataccata caaatactat atttgtatag ctgttttttg
     1621 ccaaaatgct atacataagc tacccacgat ggcgaaaaag tgatatcaaa tttttttctg
     1681 agaaacgtga taggcacagc ctatctaccc acttttttta gaaagtgggt acacgtttcc
     1741 ttatgctctt taaaattcgg gggggagtgc tgcacgtgta atcgataacc aaatttaaca
     1801 gacgaaaaat tgttggaata taaaaatatg acacccgaaa gaaaacgatc gttggaaaaa
     1861 ttaatgcaac tagtgaacga agacggcgga atttcagacg cagcttttga cgcatggagc
     1921 gaatttcatg gagaatataa ttaagtgggt ttaaatggct ctaaaatcgc ttctaagagt
     1981 tttttagctt ggaccaatga aatacaccaa aaactaaata aaacgtctta aatcgaaaaa
     2041 tacgagctct cagagcgact ttatttgtaa atgttgcata taagaaataa ttacggattt
     2101 tagacttttg taggatattt ttgaaataaa tatttatttc tgttataatt tgattgcaaa
     2161 taagtgaaca cgaaacacac gaaatcccca gagaggtcgg attctctggg gctttttttg
     2221 tgggctgagt cgccttaatt aagttttact tcttgtttcg gttattcaac cagtaactga
     2281 acaaagcgct taaaagccca acaagaaggg agcaaataaa gttagtgaac acgaaacaca
     2341 cctccttcca gtcgcaagac tgtcggatag gcgacaatca attatacaat aaaaaagcaa
     2401 aaataagcct aagtttttta ttgccttggg gttgttaggg cttgccctga ccgcctgtaa
     2461 ggcgctttag aacgaaggac atagttttta tcgaatcttg tcttagaacg gcttagacga
     2521 gctctcagga cgccacaaag gagtccctaa tgaattattt ttggcgaagg agcctaaagg
     2581 cgactgctgc aagtaaaatg tgagagcgta gcgatcactc cttcttacag cgaccatagg
     2641 gagctagatt ggcagtttaa tatcatgcgc gaacctgttt attttaaaaa gtgggtgatt
     2701 ttttggcggg aaattttgct cgcgcgcgcg cgtttatttt atttattttt taaagatttt
     2761 ttagaaccct ttttaggggg cagttgagcc ttactctccc aacggataca gaagggtact
     2821 aacaactttt tatctgagta ctaacaactt tttatctgag tactaacaac tttttatctg
     2881 agtactaata tccttttgta taataacaac caacctattt acgagttggt tgttaaaggt
     2941 tattatttat ttgtaagggg ggaagaaagg aatggatgaa aaaacggaa
//
LOCUS       DACDCJ010000165         2736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDCJ010000165 DACDCJ010000000
VERSION     DACDCJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.174"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            35..769
                     /locus_tag="IV038_002907"
     CDS             35..769
                     /locus_tag="IV038_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8343047.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            802..1710
                     /locus_tag="IV038_002908"
     CDS             802..1710
                     /locus_tag="IV038_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8343048.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1707..2249
                     /gene="sat4"
                     /locus_tag="IV038_002909"
     CDS             1707..2249
                     /gene="sat4"
                     /locus_tag="IV038_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8343049.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2342..>2736
                     /locus_tag="IV038_002910"
     CDS             2342..>2736
                     /locus_tag="IV038_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP8343050.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCP"
BASE COUNT          951 a          406 c          634 g          745 t
ORIGIN      
        1 gagataaata actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa
       61 tatatttttt caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg
      121 agaatgggag actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt
      181 aggatgcggc tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt
      241 aggtgttgat atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca
      301 gattgaatat gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt
      361 aatactaagt tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat
      421 atataggatg ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac
      481 tgctcatgga acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt
      541 ggacaattat tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa
      601 atatcataga acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa
      661 tcagattgtg gagccacagc cgccagagaa catgatggat attccgggga tggcggatga
      721 aatgcgacgc ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa
      781 aaataaacga ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct
      841 ttagcagaac aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt
      901 aatataccta aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa
      961 ccgtttatat ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag
     1021 ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt
     1081 gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta
     1141 aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata
     1201 gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc
     1261 tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt
     1321 ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca
     1381 tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt
     1441 gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc
     1501 tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt
     1561 gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat
     1621 accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc
     1681 gctgatatag aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag
     1741 atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     1801 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     1861 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     1921 agataatata tctttactac caagacgata aatgcgtcgg aaaagttaaa ctgcgaaaaa
     1981 attggaaccg gtacgcttat atagaagata tcgccgtatg taaggatttc agggggcaag
     2041 gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg
     2101 gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat cataattgtg
     2161 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
     2221 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
     2281 tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata
     2341 aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt
     2401 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa
     2461 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
     2521 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
     2581 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
     2641 agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag
     2701 gctctttcac tccatcgaca tatcggattg tcccta
//
LOCUS       DACDCJ010000166         2726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDCJ010000166 DACDCJ010000000
VERSION     DACDCJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.175"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            611..997
                     /locus_tag="IV038_002911"
     CDS             611..997
                     /locus_tag="IV038_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP8343051.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1095..1286
                     /locus_tag="IV038_002912"
     CDS             1095..1286
                     /locus_tag="IV038_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8343052.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1800..2132
                     /locus_tag="IV038_002913"
     CDS             1800..2132
                     /locus_tag="IV038_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8343053.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2042..2245
                     /locus_tag="IV038_002914"
     CDS             2042..2245
                     /locus_tag="IV038_002914"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8343054.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2429..2584
                     /locus_tag="IV038_002915"
     CDS             2429..2584
                     /locus_tag="IV038_002915"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP8343055.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         1003 a          447 c          431 g          845 t
ORIGIN      
        1 aacttacttc attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt
       61 acattttatc accgagaact catcattttt catcatgatg aatactattt acatatatct
      121 agttcaaaga caataattat tatgatccgt tattatctaa gcactataca gataatagga
      181 atttttcgtt tctaaatact ccaatttggg aatccaataa aaattttcgt tcaaatacgt
      241 gtctattcga actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat
      301 ctatacacga ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa
      361 aaagctcaag acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa
      421 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
      481 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
      541 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
      601 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
      661 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
      721 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
      781 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
      841 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttaataaaac
      901 tgataaagaa attggaaagg ttctgggtat tacaagacag ggtgcgacaa atttaaaaca
      961 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
     1021 tatgcttctt tgctcgtcct cctccttccc aaaaaaataa atagattata agttcatggg
     1081 aagaaggtaa atttatgaat actttaaaag aagatttcat tttagcaaag aatggaaatg
     1141 aagaagcagt tgtggccatt ctcaaaagat ttagcgcttt ggtccataag caatcctgga
     1201 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
     1261 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
     1321 atagtttcaa atttcgaaca ataaatttga agccatcttc tttataatta taaagtatcg
     1381 attttacagc gttctataat gattcataga atctaaaacg aatgatttta attattaacg
     1441 ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac
     1501 tttatttacg tacgaagtgg gcaggaggtc gaatccagaa ctgggccatg gtacttcaac
     1561 gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt
     1621 tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg
     1681 gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag
     1741 ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat
     1801 tgaaaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa
     1861 aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat
     1921 gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac
     1981 ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg
     2041 agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga
     2101 tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg
     2161 cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat
     2221 ggtaaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg tttgggtgac
     2281 ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct atattgactt
     2341 agcaactcgt attctacgtt actatatggt ggattcacac atgaaaaaag agattgtcgt
     2401 ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg atagaggtag
     2461 ccagcatcgg tcttttattt atcaagagct gttgaccgac caccacatca cacattcaat
     2521 gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc gatcaatcaa
     2581 atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg tgttaatcca
     2641 agattattta gaagactact acccaaacaa aagaatccat acaaaattta tgatgactcc
     2701 ttgacaattt gaagcgaaga aaataa
//
LOCUS       DACDCJ010000167         2720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDCJ010000167 DACDCJ010000000
VERSION     DACDCJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.176"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..418
                     /locus_tag="IV038_002916"
     CDS             <1..418
                     /locus_tag="IV038_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8343056.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="IV038_002917"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="IV038_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8343057.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="IV038_002918"
     CDS             1305..2459
                     /locus_tag="IV038_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8343058.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(2557..>2720)
                     /locus_tag="IV038_002919"
     CDS             complement(2557..>2720)
                     /locus_tag="IV038_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP8343059.1"
                     /translation="QIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMI
                     RHGNALIFN"
BASE COUNT          927 a          476 c          571 g          746 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat aattttggct ctttgtcaaa tgatgttggt aaagagaaat cttagaataa
     2521 ttggagatca agtggatcag ttcctcttga tcttttttag ttgaaaatca atgcgttgcc
     2581 atgacgaatc atgatgcgca atttgaaatt ataaaagcta cgaaagccat atgcatttcg
     2641 ttttaacact ttgatatggt tgttcagaca ttcaagtggg ccatttgaat aactttgttg
     2701 taaagcattt ttgatttgtg
//
LOCUS       DACDCJ010000168         2682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDCJ010000168 DACDCJ010000000
VERSION     DACDCJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.177"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..310
                     /locus_tag="IV038_002920"
     CDS             <1..310
                     /locus_tag="IV038_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP8343060.1"
                     /translation="PHQLFMADISTFEEGKNAYKAGIDFVGTTLSGYTEESKKQDGPD
                     IDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS
                     "
     gene            324..1826
                     /locus_tag="IV038_002921"
     CDS             324..1826
                     /locus_tag="IV038_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8343061.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            1839..2315
                     /locus_tag="IV038_002922"
     CDS             1839..2315
                     /locus_tag="IV038_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP8343062.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2412..2522)
                     /locus_tag="IV038_002923"
                     /pseudo
     CDS             complement(2412..2522)
                     /locus_tag="IV038_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          877 a          361 c          528 g          916 t
ORIGIN      
        1 tcctcatcag ttatttatgg cagatatttc tacatttgaa gaaggcaaaa atgcatataa
       61 agcaggaata gattttgtag gaactacttt aagtggatat actgaggaaa gtaaaaaaca
      121 agatgggcca gatatagatt tgattaaaaa attggtagaa gaagggattt gtgtaattgc
      181 tgagggaagg attcatactc cagaacaagc aaaagagata aaaaaaatag gagttgcagg
      241 tattgttgtg ggtggtgcta ttactcgacc acaggaaatt gcatcaagat ttgttaaagc
      301 attaagttag ggggagaaat aaaatgttta aaaaattttc acaattagga cgcgctttca
      361 tgttaccaat tgctatatta ccagtagccg gattactatt gggactggga ggagcgttaa
      421 caaatgagag tgctataaat gcttatccat ttttagatca accgtggttg catacaattt
      481 taagtattat gagttatgct ggaaatgcag tttttgcaaa cctagcacta atttttgcaa
      541 ttggtattgc tgtaggactt gcaaatggag ataaaggaac agctggttta gctggtggag
      601 tagcttatct agtatatact gcaactatta gtgggtttct tgaacttttt tcagcaaaag
      661 atgcaacact agatacaggt gtagtgggat caattgttgt tggtggtact gttgcatttt
      721 tacacaatcg ttatagaaaa attgaattac cacaattctt aggttttttt ggaggatcaa
      781 gatttattcc gattgtttct agtttagctg caatcattat tggttctttc ttttatttaa
      841 tttggccacc aattcaaaat ggtttagtag ttgctggtga acaaattgcc caaatgggta
      901 gtttaggaac ttttttatat ggttttttat tgcgtttaac aggtgctgtt ggactacacc
      961 acaccattta ccctttgttc tggtatacat ctttaggtgg taccgaaact gtagctggat
     1021 caacaattgc tggagcccaa aatattttct ttgcccaact agctgatcca aaccacactg
     1081 gtctgtttac ttatggtact agattttttg caggaagatt tgcaacaatg atgtttggat
     1141 taccagcagc atgttatgca atgtatcgtg caattcctaa gaaaaatcgt aaaaagaatg
     1201 gtgggcttta ttttagcggg gcccttacat catttttaac tggaattact gaaccagttg
     1261 aatatatgtt tttatttgtt gctccttggc tttatgtgat tcatgcattc ttagatggat
     1321 tatcttttta ttttgctgat atcttaaata ttcgtgtagg gaattccttt tcaggtggtt
     1381 tgatcgactt tttattattc ggtgtacttc aagggaatga caaaactaat tggttaaaat
     1441 taattccttt tggtattgca tgggcattta tatatttctt tgtttttaca ttctttatca
     1501 aaaaatttaa ggtagctatt cctggaatgg aagaagaatc agaagttcat tctgaaaaag
     1561 taataaacaa aaatacatca ttacatgaag agtctcttat cattattgaa gctcttggtg
     1621 gaaaagaaaa tattgagtct gttgaagctt gtgcaactcg tttgcgtgtt gctgttacga
     1681 atggggagat tattcaaaaa caaacaataa aagacactgg tgcaactgct gtcttcgaag
     1741 tgaagggtgg catccaagct gtttttggag ggaaagctga cttattaagt caagaaatca
     1801 atcaaatatt aggaaaagat aattaggagg tttttaatat gtttgaaatt ttcaaaaaga
     1861 aaaaacaaga aactttttat gcaccttgtt ccggaaaaat catttccatc acagaagtaa
     1921 atgatcctgt cttttcacaa aaattactag gtgatggttt tgccgtcata cctgatggtt
     1981 caaaaattta tgctccattg gctggtcgag taacaaatat atttcctact aaacatgcta
     2041 ttaatataaa aagcaactca ggcattgatt atttgattca tattggaatt gatacagttg
     2101 aattacaagg agagcctttt tcgatttgtg ttcaagaaaa tgatgaggtt actcctgaaa
     2161 cctcgttggt tgatgtggat tttgacaaaa taataaaatc tggtaaagat acttctgtta
     2221 tagttgtttt tccggagaaa aatcagttca atgatttaat aatagataat ggtcgaattg
     2281 ctcatgggaa ttcatgtgga aaaatcactt tttaaaaaag tgtatataaa aagctataaa
     2341 ataagctaaa taggatattt gtcaaatgaa gttggtgaac agaaagaact gttttctttc
     2401 tgttcatttt tttattttat tagtaatgat tccccatgcc aaatcatgat ccgtaattta
     2461 aagttataaa agcttcgaaa tccattggca tttcgttgga ataccttgat atgattattc
     2521 aaatataaaa tatagagaga agagagagag ggaagatatt tataaagggc aaagttatgt
     2581 ttactcaact taggaaatag aaatagatca atttatattc tcttgtcaat cgaatatgaa
     2641 ccttagcact atataccaaa tatagtaaaa tgctgaaaga tg
//
LOCUS       DACDCJ010000169         2664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDCJ010000169 DACDCJ010000000
VERSION     DACDCJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.178"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(248..796)
                     /locus_tag="IV038_002924"
     CDS             complement(248..796)
                     /locus_tag="IV038_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343063.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(797..1654)
                     /locus_tag="IV038_002925"
     CDS             complement(797..1654)
                     /locus_tag="IV038_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP8343064.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1940..2227)
                     /locus_tag="IV038_002926"
     CDS             complement(1940..2227)
                     /locus_tag="IV038_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8343065.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKIIPVLLCGTHEKFYEQLKKLI"
     gene            complement(2217..2546)
                     /locus_tag="IV038_002927"
     CDS             complement(2217..2546)
                     /locus_tag="IV038_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8343066.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          793 a          528 c          346 g          997 t
ORIGIN      
        1 tttttctcaa ttttacacta aaatagattt tttggaaaac tttgcaaaag aaccgattat
       61 catacacttg ttaatgaaat cacaatatct cattaaaaca caccctaaaa tcataaccgt
      121 tcctatgctc attttttgga ttttttaatc acgtaaccag aaaaataaat cccctctatt
      181 tcacgcaaat caaagaagaa ctaagtaact accgtttaaa ggtagttact tagttcttct
      241 ttgattatta tctaacttta ttattcattc cccacccttt gcgcagtgag tcttcggtat
      301 cttgttttat ctgactggta gttcttatta tttctccacg atcatctaat tctggaacaa
      361 ttggatattt ttctctgaac aagtctagtt ctttcaccac gtcattaaac aaatcttgct
      421 tatcttcttg cgtaagattc tgtttaatat actcatctaa agcttttcta agcaattcat
      481 ctcttgtcat attagtctca tacttttcta ctaaaactct ggaagtcgca tttaacaaag
      541 tcgacagttt taaaggatgc ttggtggaat aaaccttatc aaactttctg attggtgcgc
      601 caactttttt tgttcttcct ctactctgtc tttcaacatt ggaggaagtt tttatttctt
      661 gaacttggtg cacttctaat tgcttatttt cagatggtgt aattgcttgc tgctttttct
      721 ttgcttctgc ttcttttttt attttggcga gtcctttatt gttcccaaaa atatcaagtc
      781 ctttgatgtc agccaattac tccacctccg cataccaatt ctcgtgttct tccaattctt
      841 tcaaaatact gataaataat tcgtggcttt tgcgatccca ttgttttaag agacctttct
      901 ttgtcttctc catcgtaata cctgatccgt cgtatcgttt taagcgcgca tttttgagta
      961 caacattttt aatcaaatgt tccccataaa tctcttgggc ttgtttatac atttcctgat
     1021 ctactgcgtc accgctttcc aacatgaaag gaataacacc agcaacatat aaatttgtat
     1081 caaattcttt cacaaaaaag tccatatact caagatattg catcgcattt ctcatcgatt
     1141 ttacttgggt ctgtaagacg acgattgccc agtccgcaat gtacatcgcg gatttactat
     1201 atacagaaat agaaggtggg acatctaaat agatgacgtc aaactcatca aataatggtt
     1261 ctagcttttc tttcatatat ttgaattggg ataatgaatc attatattgg tctttgatcc
     1321 aatcatcata catttcaaag gacatgtctg ctggaagtat atagaggtta ttgattactt
     1381 tatagacagc ctctttaggt ggaagattgt ttatcaacac gtcagtcaca gtattttcgt
     1441 aaacaatcgg ttcaccttct ccgcctcctt cttcgttagt gaagttcata tgcgttaagt
     1501 tcatcacatc agaagtattt ccttgcatat ccaagtcaat tatcaaggtt ctttttcctt
     1561 cattaactgc tgattcaaac cctaacattt gcgcgcacgt acttttacct acgccacctt
     1621 taaaattacc gattacatat ttcttcgcta ccacgttcct acctcttttc ttttagcaat
     1681 attcatttga tactattata acatatttat ttttagttac taccaaaaaa aagatatata
     1741 tgtactacct tataattaat aaggaaaagg tagtacataa ataaggaaaa gctgaaatat
     1801 attgatttat agctttcctc aaagtaatga cgttatttaa tttatcgaga agcagagaat
     1861 actcgcgact ttagtcatgt agacgaatca gattttcgat aaaaaagaag ccgtccttct
     1921 taagaacgac aacaaatcac tagattaatt ttttcaactg ttcataaaat ttttcatgcg
     1981 ttccacataa caatactgga ataatcttgt tttctataat ctcgtaagct actctatatg
     2041 ttgtgcccgc atatttaaac ccactagacc aaatacctga taaatcccca gttttttgtt
     2101 cacccttata tggatcaatc gcaatttcat cataaattaa attcaaaaat ttttctttca
     2161 atggcttctc ttttagcttt tttaaatact taatgacatg ttttgaagga acgatttcat
     2221 aagccaattt ctgttgccaa ctcctcttta gtcattactt tagaattagt aagtgtatct
     2281 tcagcaatat ctctaaagga agaatgcatt ttgttttttc tattcttaaa ttctacaaga
     2341 atctcatctt tatcgtagcc ttctgcaatt atatcagaca aaatatcttc gctaaaatca
     2401 taaaattctt tttgtggttc aacaaattca taaagaatac cattctctac caactttgct
     2461 tcaacttcga ctccattagg aacattaaaa tctttaggta ctgtcaacat gattgaattg
     2521 ccttgttttc ttgtttttgt aatcatttaa atccctcatt tctaacagta tgtaattact
     2581 tagtaaatac attataccac gagataatgt atttacaagt aattactgtg actcgtaatg
     2641 cgtccttgct gaataaattt caac
//
LOCUS       DACDCJ010000170         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDCJ010000170 DACDCJ010000000
VERSION     DACDCJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.180"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            281..973
                     /locus_tag="IV038_002928"
     CDS             281..973
                     /locus_tag="IV038_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP8343067.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1180..1470
                     /locus_tag="IV038_002929"
     CDS             1180..1470
                     /locus_tag="IV038_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343068.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          943 a          349 c          458 g          733 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt tagtacatgt ccttcatctt cgcaggaagt gccaatatga
     1081 ttttaagaaa ggtaatagac aaaaagtcaa aaagataaaa cagagtttct tctttacaaa
     1141 gggaatgtat gttcgtataa tggtattgag gtgaaggtta tggagaaaaa atatggaaca
     1201 gtagataaga tccgtacttt gcaagttagt gccagaccgt tgatccgttt cagtttaaat
     1261 ggggtcaatt gtttgattgc ttcccatagt ttgaacttcc tagctgaagt agacgaaggg
     1321 atgaagttag tcgttacagg ttattataat aatagaaagc aatttgttgt gagatcctat
     1381 catgtgttag ggaaacccaa aattgtggta gaatatgaga aaagtctata cccaagaaaa
     1441 aaggtccgac aagatgaaag aataaactag aaaacaagcg gctgagacaa aaatgtccta
     1501 gccgcttgtt ttggaaagat ggatgcagac gatgggattt gaacccacac gagcatacgc
     1561 tcacaagaac ctcaatctat atataactac atgtatttaa gtatttgtga tgaatcacta
     1621 aactttccaa actcatttga gggatattgc ttaaagattg aacagatcaa ttcgaagccc
     1681 tattagtatt cgctcaatgg ggggatcaga ggccataaaa agagactagt cattactagt
     1741 ctcaggtagt ggaattgtag gattttaaac gttcctggag agtaccggta tgtaactcga
     1801 agaggtgatt atcatagtcg taaaagtaaa ttgaagaagc ctcgcctttc acacgagaac
     1861 gtcctggttc gacagttaaa cctaacattt ggatttttga gacaaacgaa tctatatctt
     1921 gttcgtcaat tttaaaagca atatgattgt atgtctttgg cagtttattc gacgaattct
     1981 gcattactgc aatccatagg tccttaacta agaaaaattt ttctggatat aatgaaaatt
     2041 tttttgttga ctatcgtaga tttcttttgc atcaaataag gtatttaaca attaagtatt
     2101 taaaagataa cttttaagtt atctttaagt aatgcttaaa gctatgttat actctattag
     2161 gaaatataag aaggagtata aaaatgaaca aacataatag aaaaataacg aataacaaag
     2221 aactgatctt gttactaata gggatttggg gtatcttttt actctttaat atgtatatgc
     2281 cgactatgag agcggatgac gtagtttatg caagtagatt agatgaactt ggctatttgg
     2341 gtgcttcgat agaacattat aagacatgga gttcaagaat tattatagaa ctattcttaa
     2401 tgttcttttc taaacacttt atgctatgga aattattaaa ttctacgatt atgttaggaa
     2461 ttgtagtatt actttgcaag tat
//
LOCUS       DACDCJ010000171         2481 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDCJ010000171 DACDCJ010000000
VERSION     DACDCJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.181"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..97
                     /locus_tag="IV038_002930"
     CDS             <1..97
                     /locus_tag="IV038_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8343069.1"
                     /translation="ILGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(127..1542)
                     /locus_tag="IV038_002931"
     CDS             complement(127..1542)
                     /locus_tag="IV038_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8343070.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKNKVLLQSFK"
     gene            complement(1535..1837)
                     /locus_tag="IV038_002932"
     CDS             complement(1535..1837)
                     /locus_tag="IV038_002932"
                     /inference="COORDINATES: protein motif:HMM:NF025668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343071.1"
                     /translation="MTTTVSNKGSRDGYEVVQLYIGAQYGRYIRPTKDLVAYKKVFVK
                     SGDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMVGNDSSQALTNKEEIEVRINE"
     gene            complement(1895..2146)
                     /locus_tag="IV038_002933"
                     /pseudo
     CDS             complement(1895..2146)
                     /locus_tag="IV038_002933"
                     /inference="COORDINATES: protein motif:HMM:NF014026.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
BASE COUNT          824 a          515 c          392 g          750 t
ORIGIN      
        1 cattctagga ttatataaga aaacccgaaa agaaggcact ctcttcgggt tttcggtctg
       61 tactgaaatc aaggtattat tgggaatccc agcttaaatc atagataccg taagggattt
      121 tattctttat ttaaaacttt gcaacagaac cttatttttc ccgtaaaggt aagttgctcc
      181 aaatgagacg acacaacttg ctagcataca aataaagaat attggccaga attcaaacgg
      241 aatagacaag aaaccaggaa ttccactgat accaacagaa gttgcaatga cgtgttgtga
      301 cgccataaat cctccaatta ttccagaggt cagaatggaa accacgaatg ggaatttata
      361 ctttagatta accccgaaca ttgctggctc agtgataccc atataagcag acaaaccggc
      421 agccagtcca acacctttca actttttatt ttggattaag aaattcattg caatagcagc
      481 agaaccttgt gccaaacttg acatgactcc aataggccaa atataatctc caccgttcgc
      541 catcaattga atatgaactg gtaaaaacgc atgatgcatc cctgtcacga ctaatggcgc
      601 aagaccaata ccataagcaa atccgccaat aaatggaact ttttggaata accatacaat
      661 acctgcgaca atcgtattac caatagcaat tgtaatcggc gctataataa taaatgttat
      721 aacactagta aaaagaattg caactggtgc aacaaccaac agttggattg tttcaggcac
      781 acgctttttc aagaatagct ctgttttagc taacacatag ccggaaacta gagctggcaa
      841 tacttgtgct tgtagtccta tcttagagat ggacaatcca aagattgtcc aatgtggaat
      901 atctcccgtg gttactgctt gcccgtaatt ccaggcattc atcaaatctg ggtgtacaaa
      961 gactagacct aacacaatcc caagaagagg atttccttta aagtatttta cagttgacca
     1021 tgttattaac gcaggcagaa attgaaagga agtgtttgcg ataagattta tcatatcagc
     1081 gacatcagca atattaggat atacttcaat gagtgactgc tcaaaaaaga ttcctgggtt
     1141 tgttaataaa ttattcagtc ccatcagtaa accagctgta acgatcgctg gtaaaatcgg
     1201 cacaaataca tcacccaata ttttaactgc acgttgaaac caattcaaat tcaactcagc
     1261 cgctactttt ttaacgtctt ctttgtttgc cccactcagc ccagattggc ttataacctc
     1321 ttcatagata tctgctactt taggaccgat aacaacttga aactgtccat tagcattgaa
     1381 ggtacccttc acatcttcca ggttttccaa tcctgcctta tcaactttag tgtcatctcg
     1441 caaggcaaac ctcagacgtg tcacacagtg agtcagtgct gcaatattct cttttccacc
     1501 aacaaagtta atgattgatt tagcttcttc tttattactc attaattctt acctctattt
     1561 cttctttatt agttaatgct tgagatgaat cattgcctac catgatccaa taagcaccat
     1621 catctataat ttcgttgaac tcttcatcat aatacttaaa atctttgttt gtaagttcaa
     1681 aagaaacagt cacactatcc cctgacttaa caaaaacctt cttgtaggct accaagtctt
     1741 ttgttggtct tatataacga ccatattgag ctccaatata tagttgaaca acctcataac
     1801 catctctact tcctttattt gaaacggtag tagtcacctt caaacagttc ttacccttct
     1861 ctttaatgct aattggtgac atagaaaaag tggtataact caacccatac ccaaagggaa
     1921 attgtggttc attggataca tctaagtatt tggtcgtatt aaacttttca cttgaatacg
     1981 gtctaccagt attgaaataa ttataataaa tagggatttg tcctaaatgt ctcggaattg
     2041 ttattgtaag ctttccggct gggtttattt tgccacaaag gacgtccaaa atagcattgc
     2101 cagcctctgc tcctagatgc caagcctcaa ctaatccctt acatatagat ttttaaatta
     2161 cacactagtt taaactaaaa caaacaagga aaaacttacc atttaatcaa aaaattagct
     2221 catatgaata tattagaatg taaattagac tggtcaaaat ctggtcaaaa aagaggacga
     2281 atacaggtag tcctcttttt tgttattttg atattctaaa tcatttttta ttggattatt
     2341 cccttgttcg atatagaccc aaattgatct atcaataatt atcaaactga gtcatataga
     2401 gacaaaaatt tcaaaaatga cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg
     2461 acggagtcag acacggagtc a
//
LOCUS       DACDCJ010000172         2476 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDCJ010000172 DACDCJ010000000
VERSION     DACDCJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.182"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IV038_002934"
     CDS             <1..544
                     /locus_tag="IV038_002934"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8343072.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IV038_002935"
     CDS             complement(1017..1589)
                     /locus_tag="IV038_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8343073.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IV038_002936"
     CDS             complement(1605..2210)
                     /locus_tag="IV038_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP8343074.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          780 a          492 c          381 g          823 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta gttggttctg ttgcaaagtt ttaaataaag aataaaatcc cttacggtat
     2401 ctatgattta agctgggatt cccaataata ccttgatttc agtacagacc gaaaacccga
     2461 agagagtgcc ttcttt
//
LOCUS       DACDCJ010000173         2422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDCJ010000173 DACDCJ010000000
VERSION     DACDCJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.183"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..707
                     /locus_tag="IV038_002937"
     CDS             <1..707
                     /locus_tag="IV038_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP8343075.1"
                     /translation="VMGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKL
                     IIAANQYKGKTETIFSCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSI
                     PDAARLVLSAAMNSKFGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIR
                     PGEKLYEELMTTDEIERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMS
                     GQEFIRIAKENNVVTW"
     gene            719..1723
                     /gene="neuB"
                     /locus_tag="IV038_002938"
     CDS             719..1723
                     /gene="neuB"
                     /locus_tag="IV038_002938"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP8343076.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1720..>2422
                     /gene="neuC"
                     /locus_tag="IV038_002939"
     CDS             1720..>2422
                     /gene="neuC"
                     /locus_tag="IV038_002939"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP8343077.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQ"
BASE COUNT          899 a          311 c          472 g          740 t
ORIGIN      
        1 atgtgatggg aactcaaaat gtaatagatg cagcgatcaa aaataatgta aaacattttg
       61 taggtatcag tacagataaa gtagtgagtc ctactaacac catgggtgcg acaaagttat
      121 tgagtgaaaa attaataata gctgccaatc agtataaagg aaaaacagaa acaatatttt
      181 cttgtgtaag gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta
      241 aacaaataaa aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga
      301 tgtcaatacc ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag
      361 aaacatatgt cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa
      421 aaatatataa tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca
      481 ggccaggaga aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg
      541 aaaatgataa aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag
      601 attatgaaaa aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac
      661 aggaatttat tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat
      721 gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt
      781 agctttagaa agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac
      841 atttaaaacg aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa
      901 tcttaaagag gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctcaatctga
      961 ttttattaga ataaaagatt attgtataca aaataaaatt gattttttgt ctactccttt
     1021 tgatgaagaa agtgcagact ttctaaaaca aattggtgta caaggattta aaattggctc
     1081 aggagattta acaaatttac cttttcttag aaaaatagat atgtatgggt tgccagtttt
     1141 attatctaca ggtatggctg atattcatga ggtcagtgag gctgtagatt gtttcataga
     1201 tagtccagtt actattttac attgtacttc aaattatccg gctagttctg aagatattaa
     1261 tttattggcg attaacacat taaaagaaat gttcgggata cccgttgggt actccgatca
     1321 ttcattggga tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg taattgagaa
     1381 acattttacc ttggataagg aactacctgg tccagatcat aaagcttctt tagatcctca
     1441 ggaatttgca gattttgtaa agcatattag aaacactgaa atatttttag gtgatggtgt
     1501 taagcgagca atgccttctg aacttagtac aaaagaagta tcaagaaaaa gcctagtggt
     1561 cactgagaat ttaaatgtgg gagatattct tacagagaaa tctcttacaa taaaacgtcc
     1621 tggaaatgga atagctccta agtttttata tcaatatatt ggcaaaaaag ttaaaagacc
     1681 aataagtgca gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat acttatagta
     1741 agtggaacac gagctgatta tggaatatat aaacctgttg ctgagaattt actacaagat
     1801 aattgggatg tatcatttgt tgtttctgga atgcatttat cccataaata tggatttact
     1861 aaagaattga tcttagctga cggatttaga attagtggag aagttcaaag tttgttttta
     1921 gagaacaata agggaaacat ggctcggtct gtggcattag agataaatgg tttcacaaat
     1981 atatttgaac aaactagccc tgattttgta ttattactag gggatagagg agaaatgttg
     2041 gcagctgcga tagcttgttt gtatcttgga atcaaaacag cacatattca cggaggcgaa
     2101 gtgtctggta ctgtagatga atcaataaga catgccataa cgaaaatagc atctgttcat
     2161 tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga ttcttggaga
     2221 gtttttcccg tcggagctcc tagaatagat actattttaa ataatagatt acctagtttt
     2281 agagatataa aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt tttgcaagta
     2341 tttcatccag ttgtaaccga actagaaaat atagaaaaac aaattttagc tttgatcaat
     2401 tcaatggaac aacaatctca ac
//
LOCUS       DACDCJ010000174         2401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDCJ010000174 DACDCJ010000000
VERSION     DACDCJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.184"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..101)
                     /locus_tag="IV038_002940"
     CDS             complement(<1..101)
                     /locus_tag="IV038_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8343078.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQE"
     gene            complement(175..456)
                     /locus_tag="IV038_002941"
                     /pseudo
     CDS             complement(175..456)
                     /locus_tag="IV038_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            918..2299
                     /locus_tag="IV038_002942"
                     /pseudo
     CDS             918..2299
                     /locus_tag="IV038_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          872 a          413 c          402 g          714 t
ORIGIN      
        1 aattcctgaa cttcacgata gcttagattg taacgcaggt agtaaccaac agcgacaata
       61 atgacgtctt ttttgaattg tttgccttta aaatgattca ttactctgcc ctctctgtct
      121 tttttctcaa ttttacacta aaatagattt tttggaaaac tttgcaacag aaccgaaatg
      181 agtatagtat gaattagtaa tttggttttc atctaagttt agctttgatt tactaaaatt
      241 gatatctcga tacatgttca atacatctaa tacaaatcga gaatcttctt tcggaaatgg
      301 ctcagaaaga tcactccaaa tttcatcatc ataatgatac tcgtaacctt ctctcaatat
      361 ttcgattttt ctttcataat aagatctgtt ttggtcctca aatttagcca ataactcaaa
      421 ttgatttact agtgacaatc tctgaaaact gtccattaaa tgtttcttcc tatcttgttc
      481 attaacaaaa aattataagc ccttacaacg aatattatat attaaattta attgtaggtg
      541 aacacgaatt ttataaatat ttatggtgtt aatgacaaat gatatccgac tcctcttcat
      601 acgttctgga ctatttgcta atagattaaa atttcagcat ctatttctga ccataaataa
      661 atgagctgcc gaaaagctgc caaatcagac ttgaaaataa aggatcaaaa acttttaagt
      721 ttctgacccc gtaactcaca cttcaaaatc ttacttaaaa aggagtctga aagaaaaaaa
      781 gagtattcaa aatttttttc taactagcta catcacatct atgcattgac actcaaagaa
      841 ccagaataag tcatcagaaa gcaagggggt gcggacataa tcctggacga aaaaataaac
      901 ttaggaggaa tgtccgtgtg ttttcccaca aagagcgtgt aagggcaatt cagttatttt
      961 taaaatacga ttgctcttat gctgcaacca ttcgagaatt agggtatccg tcgattggtg
     1021 cactacggaa atggtataaa gagtatttgg aatccggaga gctccatcag gagcaccgaa
     1081 aaaaatcaaa gtattctgaa gaacaaaaac gaatagcggt gaatcattat tttgaatatg
     1141 gtcaatgtta tgcacgaacc attcgcatgc ttggttatcc taatagagag tccttgcggc
     1201 agcggtgtga agaacttgca ccaggagcta gaaaactccg aaaaagtgcg gtaaagttaa
     1261 cacaagatca aaaagaagct gtgttgaaaa aattctataa accgcaaact aataggaaaa
     1321 atttagcaga atctgaagga attagtagag ttactctata tcaatggaaa aacacggttc
     1381 ttggaaagga ttttccattg cgtatgactt taaaaaatca ggaaaaacaa aaagagctac
     1441 ttttaaagga agtagaagac ttacaaaaac aagtacacca attacagctc gaaaaaacat
     1501 tactcgaagg agcggctgaa ctgctaaaaa aagaaaaagg cgtcaatctt ctttgtctat
     1561 caaatcaaga aaaaacgata ttgattgatg ccttacgaaa tcagttcacc ttgaaagagc
     1621 ttcttcaaca gctccagttg cctaaaagca gctattttta tcaaaaacaa gcacttgaga
     1681 aaccagataa gtattacaaa gaacgtcaat taattatcac gattttcaat cataacttct
     1741 gtgcgtatgg ttaccgtaga attcaccaag ctttaaaaaa tatgggtaaa aagctttctg
     1801 aaaaagttgt acgtagattg atgactgagg aaaatctatt tgttaaattt tctagacgaa
     1861 aaaagtattc ttcgtatgct ggggaaattt ctcctgcaca ccccaattta ttaaatcgga
     1921 attttaaagc aaagaagcca aatgaaaaat ggttaacaga tattactgaa tttagaattc
     1981 ccgcagggaa aatctactta tcaccacttg tcgattgtta cgatggtgct attgttagtt
     2041 ggactattgg aacaaagcca acttgcttca ctctatgtct aaaaagggtt attcaccaga
     2101 taattcagcg tgcgaaggtt tctttggacg attaaaaaac gagatgtttt atcaacgtga
     2161 ctggaagaat acaacaatca atcagtttat aaacaaattg gatgattata ttcattggta
     2221 taataatcaa cgaattaagt tatctttagg agggctaagc cctcttcaat atcggaagaa
     2281 gcaaagatat atttgttaaa aaggtccaaa aaaatatccg cacccccttt ttttgtaaaa
     2341 aactattgca caatacaagt ggcctacggc tatcgaaatt tccataactt tcgagcaaga
     2401 a
//
LOCUS       DACDCJ010000175         2323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDCJ010000175 DACDCJ010000000
VERSION     DACDCJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.185"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            55..>2323
                     /gene="esp"
                     /locus_tag="IV038_002943"
     CDS             55..>2323
                     /gene="esp"
                     /locus_tag="IV038_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP8343079.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT          839 a          353 c          472 g          659 t
ORIGIN      
        1 atttatcttt tattcttact aagaaaataa ctattaaggg agttgatttg aataatggtt
       61 agcaagaata ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt
      121 atcaaaaaat taagtgttgg ggttgcatct gttttggttg gtgttggtct tgtcttggga
      181 acaactgaac tagttcaagc acaagatgaa atttctccaa gcaccccact tgaaacagcg
      241 ataagctccg tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct
      301 ggttatacga aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga
      361 gacacactaa aaggcaatac aattggattt gaagtttacg gaaaacataa tattgcagca
      421 tcaactaaaa actgggaaat ccgtcttcaa ttagatgaac gattagctaa atatgtagaa
      481 aaaattcagg tcgatccaaa gaaaggaatt ggatcttcta gacggacctt tgtaagaatt
      541 aatgattcgc tgggtagacc tacaaatatt tggaaggtca attatattcg tgcaagtgat
      601 ggattatttg ctggggctga aacaactgat acacaaactg ctcctaatgg ggtaattacg
      661 tttgaaaaga gcttagatga aatttttaaa gagataggca tagacaatct caagaccgat
      721 cgtttaatgt atcgaatcta cttggtaagt catcaagacg atgataaaat tgtaccaggt
      781 attgatagta cgggctactt tttaacagat tcagatgatt tctataatag cttagatgta
      841 tctgaaaata atcctgacca gtttaaacat ggatcagtaa gtgcaaaata tgaggaacct
      901 aatactcaaa caaaagatgg ttcaggatcc acaggtgcta atggagctat aatcttagat
      961 cataagttaa cgaaaaatta taatttctca tattcagctt ccgcaaaggg cacgccttgg
     1021 tatgctaatt acaaaattga tgaacgatta gttccatatg tagcgggtat acagatgcac
     1081 atggttcagg ctgacaaagt aacatatgat gtttcttttg aatcagggaa aaaagtggct
     1141 gatttagcaa ttgaacggcg caaagatcat gaaaattatg gtgtaggttc aattactgat
     1201 aatgatctta ccaaacttat tgattttgct aatgcaagtc cacgtccagt cgttattcga
     1261 tatgttttac aattgactaa gccattagat gaaatcttag aagatatgaa agcaacagca
     1321 caagttgaag aaaataaacc atttggtgaa gatttcatct ttgattcttg gttgtcggat
     1381 acgaataaaa aattaatcca gaacacttat ggaacaggtt attattattt gcaagatatt
     1441 gatggtgatg gaaaccctga cgataaagaa gagagcggag acacgaatcc atatatcggg
     1501 aaacctgaat tagaagaagt atatgatgtt gacacaacag ttaaggggaa agtattcatc
     1561 cacgagttag cgggaacagg tcacaaagcc caacttgttg ataaagaagg tactgtatta
     1621 gcagaaaaaa ctatcgctcc aaatgaaaaa gatggggctc caatttcaga tactgtagaa
     1681 tttgaattta cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa
     1741 atcgtttctc caggttttga taaaccagaa gaaggttcaa ccgttattaa ggaatcacca
     1801 aaagcggttg ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt
     1861 cggaataata aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact
     1921 tctaacgtta ctgatagtac gaaaggtatt gtaactgtta agatcggaaa tcgaactttc
     1981 gacgtggatg tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac
     2041 gtacctataa caacaactcc agaaacgaca gttcaaagtg gaaaacctac atttgataaa
     2101 ccagatgttc ctttagctaa agatgccttt tcaattttag atgtctataa taaggacttc
     2161 ggtaatgcaa gtgttgatgc aaatactggt attgttacat tcactccagc taaaggtgta
     2221 ggagaatcgg agccgattac tggaacaatt cctattaaaa ttgtttacca agatggttct
     2281 gtaggcacga ccgatttagc agtaactgta agtaaagata ttt
//
LOCUS       DACDCJ010000176         2319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDCJ010000176 DACDCJ010000000
VERSION     DACDCJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.186"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            19..2172
                     /locus_tag="IV038_002944"
     CDS             19..2172
                     /locus_tag="IV038_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP8343080.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          795 a          414 c          519 g          591 t
ORIGIN      
        1 taggcggaag gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg
       61 ttagaaaaaa taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat
      121 gttccttttc gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt
      181 gctagattgg gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct
      241 acttctacga ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga
      301 aaagctttag ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt
      361 acggctaaag atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta
      421 gatgaaaatc tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg
      481 aaagaagcca caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat
      541 agtgcgatgg tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa
      601 attgctacca tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa
      661 aactctggtg ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca
      721 ggagtttcga ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt
      781 attcttggca gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac
      841 ctagcaaaag gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat
      901 gaagatgtcc tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat
      961 gtttcgaatc aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg
     1021 aatgcagaat tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc
     1081 aacaacggca aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca
     1141 ggagaagtgc tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag
     1201 aatgctttag gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact
     1261 ttgacagcag gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg
     1321 attcgtctgc aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct
     1381 cttgatgcag tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta
     1441 aagatgttag gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct
     1501 aaagcttatc agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg
     1561 atcgatttgc cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc
     1621 aatgcggata atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga
     1681 cagtttgaca catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc
     1741 gggaaacgag tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac
     1801 ttgggtgaag tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca
     1861 gaaaatatgg cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac
     1921 acaacagcca aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca
     1981 gaaacggttg cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac
     2041 ggaccaacag acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa
     2101 caagaccaca taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc
     2161 atggcaaaat aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt
     2221 ttgtgaaaat agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt
     2281 ttgaacaccg tttactcggc aaaaaaggtt gtgacaaaa
//
LOCUS       DACDCJ010000177         2301 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDCJ010000177 DACDCJ010000000
VERSION     DACDCJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.187"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            196..>2301
                     /gene="acm"
                     /locus_tag="IV038_002945"
     CDS             196..>2301
                     /gene="acm"
                     /locus_tag="IV038_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP8343081.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVII"
BASE COUNT          909 a          350 c          445 g          597 t
ORIGIN      
        1 aaaaaataat ttacattagg aaaaaccttg taaaaacagt tttttggcat atttattttt
       61 tataaatatt tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg
      121 aggaattgaa gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata
      181 ggggagatag aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt
      241 actaatataa catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc
      301 agtaatgtca cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa
      361 gtaaaattct cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc
      421 aattggccaa gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg
      481 attgaaaata aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt
      541 aatgaaaata ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc
      601 agaaacgtaa ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat
      661 aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat
      721 tataaaacag gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt
      781 aacaataacg gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga
      841 caaagattag atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt
      901 tatagaggag aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat
      961 ttttcagtaa ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg
     1021 aaaataatgg tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat
     1081 cattcggaag cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat
     1141 catacagtta aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg
     1201 aagattttca aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta
     1261 agacgagcag atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa
     1321 cagggagaag ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa
     1381 gcacccaatt ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac
     1441 gaaaatgata cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc
     1501 acagcaacta aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta
     1561 ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac
     1621 aatggaataa cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa
     1681 tatacgttta aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat
     1741 caaaaaattg aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca
     1801 ggacaaaaaa catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca
     1861 gtaaatctat tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat
     1921 tggcgctatc gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact
     1981 gtaacggaag acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac
     2041 agttatacac cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa
     2101 aattttccag agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa
     2161 atatttgctc atacaggaga atccagcgaa caaagaagca attatattag taagagagct
     2221 gtacctaaac agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa
     2281 tctagttttg ctgtaatcat t
//
LOCUS       DACDCJ010000178         2290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDCJ010000178 DACDCJ010000000
VERSION     DACDCJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.188"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(289..606)
                     /locus_tag="IV038_002946"
     CDS             complement(289..606)
                     /locus_tag="IV038_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP8343082.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYEMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(607..861)
                     /locus_tag="IV038_002947"
     CDS             complement(607..861)
                     /locus_tag="IV038_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP8343083.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1121..1339)
                     /locus_tag="IV038_002948"
                     /pseudo
     CDS             complement(1121..1339)
                     /locus_tag="IV038_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          744 a          417 c          336 g          793 t
ORIGIN      
        1 tctcatgagt aaagtcacga aatttttggg aatagagcgt acttgatatc ttattttttc
       61 actaatagca gtgtctctta ttcaccaagc gatccccgta gttcctacgg ttcttaattt
      121 ttctctagac taatgattgt atctttagac cttcggccat aacatggagc aaaataaaaa
      181 ggtactaagt cttatatatc aatcttcgag taaccgatgg ttaacaacca tatcgagcgg
      241 cattaatcac ccacctgtag aggatgagcg acttttgcct aattattgtt aaaagcctag
      301 tttcatttgt ttagattgga aaatacgtcc tacaagtatc tctttttttt catggtcgat
      361 ccgataaaat aaggcatagc ttttgccgat cacgatccga tatgttggga tatccatttc
      421 atagatttcg gaaacttcct catgcatagt tggaaaattt ttggttagtt ctaaagcctt
      481 ctggatcgca cctacaaatc tttttatgct ggctggagaa agagcaagct cattttccca
      541 ttcttctatt atcgtttcaa ggctggtctg gaaactttta gaataaacta gcgtgtactc
      601 tttcatctat tcactccagc cgtattttcc gaaagctttt accatttcaa tttctgaaac
      661 tgttaaacct ttctcaaagt cttggaaact ttcttcaatc aatttgcgat cgtgttcttt
      721 tgctatttga gtagtatcta atatgaataa gttgccttct tgtttggctt ggatagtgtc
      781 tccgcttttt aatccaagag agcttggaag gatcactccc acgctattgc cgatctttct
      841 aacctttagt tcttttccca tgttatcaca ctccttttag tttttgataa ataaattata
      901 acatgttata acaacaattg ccaacggtgt ttcttaaggg attccactcc atattattat
      961 tggacaaaat aagccactta gaacgctgaa aacatataca aacgatatca aaatacgagt
     1021 gaactactca ccatacactt ccgttaacat ccatttaagt aggtattttt attatatata
     1081 gatttaacat atgtcggtcg gttgattgat atagctaaag ttataactca accgaagccg
     1141 atttatctac acgaataaag ttgcgagtgt tctcggctaa ttcataaaaa ttgttatgtg
     1201 ttccatatag caatactgga ataactttat tttcttgaat ctcagaagca actctataca
     1261 cggtgaccgc atacttaaat ccattagacc aattacctga taaacctcca gttttttgct
     1321 cttacttaaa tggatcaata gaaatttcat cacaaaccta tagaggatga gtgacttctg
     1381 cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag caaaaaatat ctagaatttc
     1441 atttcctcaa aactgatcga tattgtatcc aaaaatatat actacctaaa ataggtagta
     1501 tatattaaaa tatgtgcttt tgttagaaaa atcaatacat aaattatcat atctgtatat
     1561 gtcatttagg tattttagtt atgttataat agttttgctg tctgctaccc cggcctaatc
     1621 tggcaagggg aggaggagat ataatgaaat acctttttga ttttgttttt gcaatcaagc
     1681 catttgcaga ggtaactgca cactacatct gcaagtggct tgattgcaaa attgccaaaa
     1741 aagacagtaa gcctacccgt taattctata atatagaaac gtaaaaaaaa accccatcag
     1801 cctgcacgct gatggggttt ggtgatataa tgaaatacct ctttgttact cataattata
     1861 ccataattag tcacgaatgc aaccaataga aaaaactact tagaacggta gatcatcgct
     1921 aataacaatg cattgtccac caaatggatt ttgtgaagca ttaaatacat tgttattaat
     1981 tgtcgctgct tttaactagg cctctcccct tctattataa aagaagatat atatcatttc
     2041 ttaattacct actaccttaa accaaaattt aagtactacc taatttaagt agtacttaaa
     2101 taatctaaaa aatagttatc ccaattgtta tgattttata atacgattat ttgtaacaaa
     2161 atgaatatta tataattaaa cactaccata tttaggtagt gtttaattat tagcaaaaca
     2221 aaaaatattt attctattct cttaacttca actaagttat gctgcacatc tttatataaa
     2281 tcatatgcaa
//
LOCUS       DACDCJ010000179         2188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDCJ010000179 DACDCJ010000000
VERSION     DACDCJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.189"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(500..1792)
                     /locus_tag="IV038_002949"
     CDS             complement(500..1792)
                     /locus_tag="IV038_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8343084.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          707 a          417 c          346 g          718 t
ORIGIN      
        1 tatccactta tattataaat tcacatgatt atcgaatttt attaagttag tttttcttgt
       61 aaatctctgt tcccaatgaa actcgagaca cttattttaa agaggaaaca atgtggtatt
      121 taaatagccc tcttgtatgg gctactctgt gatacttatt agccattaaa ctattttacc
      181 cacatagcat atattttgaa atcgttcatt ccgtaaattc aactaaatat gcctaaaaaa
      241 ataaaaaatc taagatagaa taagatttat tcagtcttag atggatttat agcaactttt
      301 tcgtaacaac tttaaaaaat acgaaaagaa agagctgttt tactctgact acgttgctaa
      361 attgtcgttt aatcattcat ttaaaccaaa tcttttttta tagaattcgt tttaatcctc
      421 ctgaatccaa aaactatgaa aaccgtaatt aaatagttaa gagtttttaa ccaccataat
      481 tctctcggct attttgaaat cagataaatt ttgatcggac acatagttga ataaacctac
      541 cgttgttacg gaaggtaaat cgcatacttt tcattctaga ggagaaggtc tcgttgatca
      601 ttaactgatt aagcaccata gcccagtttt gaattcgacc acctgaccac ttcgcgtgta
      661 attctttcgt gcgtaaataa agtagtttta ggagtgcatt ctcattagag aacgctcctt
      721 tttttgtgac ttttctgaag ctggagtgga cactttcaac cgcattggta gtgtacataa
      781 tttttcgaat ggcactacca taatcaaata gttgttcaac atgtgcaaag ttccgtttcc
      841 agacatctac agcaccagaa taatgagacc accgattttg aaaactgtca aaagcagcat
      901 gtgcagcgtt tagagaagaa gcaccgtaga actttttcat atctcggcag acttccttat
      961 agtccttact tggaatatag cgcaatgcat ttcgaacaag atgaacaata cagcgctgaa
     1021 caattaccga tggaaagatc gcttttgcgc cttcttcgag gccagaaaca ccgtccatcg
     1081 aaatgaaaaa gacatcttcg acaccacgtg ctttcagttc atcaaatact tgcatccagc
     1141 gatttttaga ttctgtttga tttaaccata atcctaaaat ctccttattt cctttgagat
     1201 catagccaag aatggtgtat acagcatatt ctttggcttc ataattttct cgtaaagtaa
     1261 catacataca atcaacgaat agaaaggcat aacactttgc tagggggcgg gcttgccatt
     1321 cttccaattc aggaaggaca gcgtcagtga tatctgaaat catttcatgg gaaatatcaa
     1381 agccataaat agcttcgacg gttgcggcaa tatctcgttg actcattcct cgtgcataca
     1441 tggaaagaac cttcccttcg atgtcggaga catctcgttt tctcttagga attaactctg
     1501 gttcaaagga agcttcccgg tctctaggaa catcaatagc tacttcacca aaactggttt
     1561 taagcgtttt agttccatag ccatttcgac ggttatcgtg ttccttaggc tctttagaat
     1621 gggcatcata acctaaatga ttattcaatt ctccttgaag cattttttca aaaaggggcc
     1681 caaacacatc tttcaaagca tcttgcatgt catcgacaga ttcaggttga taggcattca
     1741 gaatggattc agctaacttt tcggcatcag gatttctttt ctttctagcc atcgtgtaat
     1801 cacctcttga tcttattgta gaaaaagaaa aaccgcgaag caaccacctg cctaggatta
     1861 atggttactt acacggttta tattacactc tcatcattaa aaaagtatct ttcgatatta
     1921 ctaaacaatg aaattatcta tttgaaattt aaatctgata atatattgag tgttccaatg
     1981 agggctttga tcctattatc catatctttt tcagttttaa tattgataca cattataaga
     2041 aaaagaaact tttggttaat ttatttcatc ccagtgcaaa atataaaaaa gtaaaataga
     2101 ccataggcaa tagattaaca attaatataa tacaagcaaa tattctattc attgtatttc
     2161 tgctttcaaa aagttctaat attgaaag
//
LOCUS       DACDCJ010000180         2114 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDCJ010000180 DACDCJ010000000
VERSION     DACDCJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.190"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            57..845
                     /locus_tag="IV038_002950"
     CDS             57..845
                     /locus_tag="IV038_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP8343085.1"
                     /translation="MVLLESMPDGYIYSNILLKLYLRSLKHEGKLMFNDRIPFNSTML
                     ATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDIQSFIGKSSTEADRKRKYRKEI
                     EEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEKDIENVTPSKKSKAKPVRHKYG
                     EYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSE
                     SKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            857..1126
                     /locus_tag="IV038_002951"
     CDS             857..1126
                     /locus_tag="IV038_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343086.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1288..1590
                     /locus_tag="IV038_002952"
     CDS             1288..1590
                     /locus_tag="IV038_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343087.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1587..1748
                     /locus_tag="IV038_002953"
     CDS             1587..1748
                     /locus_tag="IV038_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP8343088.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1745..2050
                     /locus_tag="IV038_002954"
     CDS             1745..2050
                     /locus_tag="IV038_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343089.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          821 a          327 c          485 g          481 t
ORIGIN      
        1 acaacaaacg atactactat ttaaaactaa aagagaactt tttcgatagc gacgagatgg
       61 ttcttttaga aagtatgcca gatggataca tttattctaa tattcttctc aaactttatt
      121 taagaagtct gaaacatgaa ggcaaactaa tgtttaatga caggattcca ttcaattcta
      181 caatgcttgc gactattaca agacactctg tgggagttgt agaaaaagca gtacaaatat
      241 ttcgtgattt acaacttatt gaggtattag ataacggagc aatttatatg tctgatatac
      301 aaagttttat tgggaaatct tcaactgaag ctgataggaa gagaaaatac agaaaagaaa
      361 ttgaggaagc aaaacagaat ttaataacaa gtggacaaat gtcggacaaa tgtccggaca
      421 aaactacacc agagttagag atagagttag agaaagatat agagttagag aaagatatag
      481 agaatgtaac gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
      541 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaatagaa ttccctaatg
      601 actaccaaga gcgaatagaa cggctatctg agtattgtga atcatctggt aagacttata
      661 aaaactattt ggcaactatt cgaagctggg caagaaaaga aaaaagtgaa tctaagaacg
      721 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgagtgg gcaatcgacc
      781 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
      841 tctaagaggt gggaaattga aaatcgatta tctagaacta attaatgaaa tagcaaagta
      901 taaaactggt gaggaaatag aaatcctgag agacgtatat gatcaactcg aagaagctgg
      961 aatcgaagga attaagaatg atcgttcgag ttggagtaag ctcagatact atttcgcact
     1021 ttatatcgat acaacacaat taagaaattt agcatatacc aaattactat ttgttgattg
     1081 tgttaagggc ttgcaaaaac atcttaatga acttgagcag gtgtaatcag atggacctaa
     1141 agacatttac agcacagatt gaactaatgc atcaagaagc tttaagacaa agtgtatcgt
     1201 acgaagacaa gtggctcaac acgttccacg gcggacgtga gagcgcactt gatcaagtac
     1261 tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca gcgaattatc
     1321 gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga agtgatgagg
     1381 cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa aatcgcaatt
     1441 tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag tctcacaggc
     1501 ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc taagaagaga
     1561 acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa atgcgtattc
     1621 gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg gtcaatgaaa
     1681 cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc catcgattgg
     1741 aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa ccaagtcaga
     1801 atcttggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta aatcaatgtg
     1861 gactgaaaag cccaaacgga gaacgccgag aaaaattgca atctggcatg atgaccgaat
     1921 tttagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca aaaacattat
     1981 ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta ggtatgtgga
     2041 ggagagataa tggatctcat tacacaatac agtgacatca tcctcaagaa aatcatgatg
     2101 aagattcaga aaga
//
LOCUS       DACDCJ010000181         2102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDCJ010000181 DACDCJ010000000
VERSION     DACDCJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.191"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(117..707)
                     /locus_tag="IV038_002955"
     CDS             complement(117..707)
                     /locus_tag="IV038_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP8343090.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="IV038_002956"
     CDS             1230..1391
                     /locus_tag="IV038_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343091.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="IV038_002957"
     CDS             1410..1904
                     /locus_tag="IV038_002957"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP8343092.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          753 a          330 c          360 g          659 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aa
//
LOCUS       DACDCJ010000182         2077 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDCJ010000182 DACDCJ010000000
VERSION     DACDCJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.192"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(40..966)
                     /gene="cysK"
                     /locus_tag="IV038_002958"
     CDS             complement(40..966)
                     /gene="cysK"
                     /locus_tag="IV038_002958"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP8343093.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1106..1993)
                     /locus_tag="IV038_002959"
     CDS             complement(1106..1993)
                     /locus_tag="IV038_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8343094.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIRTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          574 a          467 c          354 g          682 t
ORIGIN      
        1 gttttttaca taaacctatc tggagaaaaa ccaagtgcct tatatctctt ggtaaagggc
       61 tgtagataaa taacgttcac cattgtcggg aaccacagct aataccttct tgccttttcc
      121 taattctttt gcgactttta gcgcggcagc aattgcagcg ccagatgaga tgcctactaa
      181 aatcccttct tttcgcccaa cttctctagc tgtttccatc gcttcgtctc cactaataga
      241 aaggacttta tcatacacag aagtatccag tgttttagga acaaaacctg taccgattcc
      301 ttggattttg tgaggtcccg cttctttccc ttctaaaata gcagattctg ctggttctac
      361 cccgattaat tcgattttcg gatacacacg ttttaattct cggccagcgc cagtgatcgt
      421 tcctcctgta ccgatacctg caacaaacgc gtccaagcca tttactccaa atgcttgatg
      481 tatctcagga ccagtcgtct tctcatgcac aagaggattt gcttcatttt caaattgtaa
      541 tggtaaaaag tagccgtttt cttttgccaa acgttctgct tctctgattg atccagaaat
      601 accatccgca ccaggagtca aaatcaactc tgcgccgtac cctttcatca ataaacgtct
      661 ttcgatactc atcgtctcag gcatcacgat gattacttta tatccttttg caactcctac
      721 catcgacaat ccgattcctg tatttccaga agtaggttca atgatagtat cacctggttt
      781 cagtagacca tcgtgttctg ctttttctat catacttaaa gcaatacgat cttttacact
      841 tcctcctgga ttaaaaaact caagctttac aaaaacatct gctgaatctt ctggtacgat
      901 tttatttaat ttgacgattg gcgttttgcc aatcaattcg gtgactgagt tatagatagc
      961 tgtcatcttc aattcctcct tgtctttgct cttcttactt atcataatac aatctgttca
     1021 tttatggcga gtgattcgtc tttgactagc aaaaaaaaga caaagatata ccatatatgg
     1081 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
     1141 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
     1201 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
     1261 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
     1321 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
     1381 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
     1441 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
     1501 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
     1561 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
     1621 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
     1681 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
     1741 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
     1801 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
     1861 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttcgaatt aaaggagcaa
     1921 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
     1981 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
     2041 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DACDCJ010000183         2029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDCJ010000183 DACDCJ010000000
VERSION     DACDCJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.193"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..424)
                     /locus_tag="IV038_002960"
     CDS             complement(<1..424)
                     /locus_tag="IV038_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP8343095.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="IV038_002961"
     CDS             complement(533..1564)
                     /locus_tag="IV038_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8343096.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          626 a          330 c          349 g          724 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgt
//
LOCUS       DACDCJ010000184         2014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDCJ010000184 DACDCJ010000000
VERSION     DACDCJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.194"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IV038_002962"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IV038_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP8343097.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IV038_002963"
     CDS             852..1040
                     /locus_tag="IV038_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP8343098.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IV038_002964"
     CDS             1053..1610
                     /locus_tag="IV038_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP8343099.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IV038_002965"
     CDS             1642..1884
                     /locus_tag="IV038_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP8343100.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          737 a          309 c          407 g          561 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaa
//
LOCUS       DACDCJ010000185         1975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDCJ010000185 DACDCJ010000000
VERSION     DACDCJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.195"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..195
                     /locus_tag="IV038_002966"
     CDS             <1..195
                     /locus_tag="IV038_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343101.1"
                     /translation="SFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSFYNAFV
                     SKHVEEYEVLMYLKEKKTSN"
     gene            402..602
                     /locus_tag="IV038_002967"
                     /pseudo
     CDS             402..602
                     /locus_tag="IV038_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1089..1268
                     /locus_tag="IV038_002968"
     CDS             1089..1268
                     /locus_tag="IV038_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343102.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1314..1919)
                     /locus_tag="IV038_002969"
     CDS             complement(1314..1919)
                     /locus_tag="IV038_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8343103.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          635 a          343 c          350 g          647 t
ORIGIN      
        1 tcatttctaa tactaggtct tccacaagca gtgtttcctg aaatgttttc tggagactgg
       61 ggtattgtca gctattctat attaacattg attgctatgg ttttaggtac ttcattttat
      121 aatgcttttg taagtaagca tgttgaggaa tatgaagtat taatgtactt aaaagagaaa
      181 aaaacaagta attaatatga gaacaccacg catagttttt attttgagat attctagaat
      241 gaaacacatt aagaacgaaa gcaaagcaaa agacgccaga acttctacta ccttctaagt
      301 tttttcatat ttgtttgtta tgctatacat agatctgcat aattagactt gcgctgcccg
      361 ttattgaagt ttcaggcagc gctctttttg ggggatcgat aatggaagaa gaaaaaatat
      421 ttgagaaacg ctggcagcta gcatcaagtg aacaaagagc aagatacaat aatttgatca
      481 cctcctaccc cactgttgac tagacatata aggaaaagaa atacttactt tggctgtgcc
      541 agttagatat agataccttt gaaacatttg aagtgatcct agataaaatt aaacagaatt
      601 gatcagaaac aagcgaagcc ttctctcggt tcgcttgttt tgctatttat tagttggctc
      661 agggcaactc taagctcgtt tgaaagaaaa ccgatggata atccccttgt ttgtaccact
      721 ttagaataca tggatgtacg tattgccatt gactcctatt agttactgaa aaaaaattta
      781 aacaagttga gtattgcaga taaatagctc cagtgaggtg aataactgct ccactcaagg
      841 caatagatag ctccatccag ggcaacaaac tgctccactt tcatatttaa tagatgaata
      901 accttctgta taatagaaag gcataccttt cggtatgaca acttatacag aatgttattt
      961 tattggttca ctatcgaaaa attttggagc tttatttttc cggtatttct caacgaacga
     1021 ttagtttaac tgtgggaagc tccagacata cgattcgaga agttacttct cgagcagaca
     1081 ggaagggggt gactgaacta accgctgata tgagcgatac ttggctgcag gaatatcttt
     1141 ttccggaaaa acaaacaaca gcacgaggtt accttcctgt agactgggaa tatgttcata
     1201 aagaactaat gaaaaagaat atgacattga aactccttca tagagaatat ggggtacatg
     1261 gaacatagaa acgaaaatgc acggtagaaa agcgaatgaa tatagtataa attctacaaa
     1321 tcattcgctt tctttatttt atatatggtt ggtcgagaga gaccattttc tttggcaatt
     1381 tctgcaactg gaactccttg atttaacatg tccaccacag ttttaaagac taatcgtttt
     1441 tgcggatctg ttgagttctc agaatattca attggtcgcc ccttatatcg accagctttt
     1501 ttggccaata atactccttg ccgctgacgt tcacggattt tctcacgttc gttttgtgca
     1561 atataactta gcaatgataa aaacatatcg aacatagcag tatctaggag ttcatttcca
     1621 gtattaaaat ctaaaaactg tgcatctagt atttttaatg ctactttttt ctgtttcata
     1681 aaggtaactg tattttttat gtcttcataa tctctgccta ggcgatccag tgatgcaaca
     1741 atgacacaat caccttctcg gatataattt agtaattttt gaaactcagg tcgctggata
     1801 tctttaccac tggctttttc ttggaataat ttatcaatgt taaggtcttc catcattgcc
     1861 aattgtcgat ctaaattttg atcttgtgaa gacactctaa tatatccaat ttttgccata
     1921 aaaactcctt ttaaaaaatg atataattta catgtaaaat ttacttgtag tttta
//
LOCUS       DACDCJ010000186         1859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDCJ010000186 DACDCJ010000000
VERSION     DACDCJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.196"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IV038_002970"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IV038_002970"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP8343104.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DACDCJ010000187         1838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDCJ010000187 DACDCJ010000000
VERSION     DACDCJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.197"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            36..1799
                     /locus_tag="IV038_002971"
     CDS             36..1799
                     /locus_tag="IV038_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343105.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT          595 a          343 c          312 g          588 t
ORIGIN      
        1 tacgtataaa gtggagcata atcattatat tatcattgtt atttttaaca ggaattgctt
       61 atttctttta ttttattttt tctcctgcgg acagcaatca aatgaaagaa ataaaagatt
      121 cgtgggtttt ctatacaaaa gatgatcctg attttaagtt tgacagcaag tacactcgta
      181 cgattcctac cgtggataaa gacgaaacat tcattatgga aacgacactt gaaaaaccgt
      241 tggacgaagc aaatttacta atcaaaggaa accatcaatg gattactgta tatctaggcg
      301 aaaagatttt ataccaacgg aaagattatc aggaagaaag caatcccgga ctctcattgg
      361 ctgtgataga tttaccagca aattatatcg gaaaaaaatt gacccttaca gtctcatctc
      421 cttatcagaa ctatgctgga ctgccaccga aagtttatct aggaacaact ggaccattga
      481 ttagttttat ttattcacaa tcagtgccgc aagtattgac gatgataatc gctgttttta
      541 tcgctatagg gacctttttc tatacgatct acttgatgta taaacaaaaa cgtcttgaat
      601 actcacttat tctcttaagt tgctttgctt tggcattagg atttgaagct gtatcagaag
      661 atattttaag tggtattctt tttgaaccat tcgttcattc ttttttatct catttattta
      721 ttatcttaac gtcagctttc atcatctgct actattggag ccgaatgttt tactataaaa
      781 aatggtacgg aattttttgt acgttccaac ttttgatttt tatcggcgtt ctgctttatg
      841 caacttttac tcctgcagaa cttcctgaat taatgccgat tgctaatatt gctagtgtta
      901 taagcacttt agtcacttca cttgcatgca ttggcgaagc atataagaat aaccgttttt
      961 atgtagcttg tacaccttgg atcgtcttgg tagccattat ccattgcttg atttacatcc
     1021 aaacggcatt aggtatctat caaacaacga tcaattggtc tacgatttta tatattatca
     1081 ttctaatcgt tattatcagc tacaatctaa tcgatcacat cttaaatacc gacaaagatc
     1141 ggagacaagt agatttctta aaaatcaaaa atgatttgtt ggagcaacat tatgagcagc
     1201 ttcgacagca tatccaagaa gtaaatacac ttcgcctgga atttgtccaa gagatggaga
     1261 atctgcaaga tttaatgcat acggaccaaa caaaagctaa aaactatgtg gaaaagatat
     1321 tagaagaagc aaaaaatttc gaaatgctgt gttctttttg caaccaccag gtgacaaatc
     1381 ttatcttagc gcgttaccag aagttagctg ccaaacggaa tattcagata accttccaag
     1441 cagatcttcc cgaagaactt cctatcaacg atgacgatct ggctcaactt ttgatccacg
     1501 ctttagagca ttcctttaga gaaactcatg ccatcgaaaa tccactgtat cgaaaaattt
     1561 atttgtcgat acacgaacaa gaggaacatt tcgtcatttg ttgcgaacat agtgcacatt
     1621 acgatacaaa cctatttagt cgaggcatca cagaagaatt agatgatcag gaacgttttg
     1681 acttaatgat gatgaaagac gtagcggatc gttacacagg aacactaacg caggaaaaag
     1741 atactttcat tgatcggatc actgttcaac tctctcgaaa ttacccgaat cctgtataaa
     1801 aaatagcgga atccatatat gtggattccg ctattttt
//
LOCUS       DACDCJ010000188         1787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDCJ010000188 DACDCJ010000000
VERSION     DACDCJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.198"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            123..1292
                     /locus_tag="IV038_002972"
     CDS             123..1292
                     /locus_tag="IV038_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP8343106.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1617..>1787)
                     /locus_tag="IV038_002973"
     CDS             complement(1617..>1787)
                     /locus_tag="IV038_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP8343107.1"
                     /translation="KISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQD
                     EEFFIFKIKIFS"
BASE COUNT          644 a          285 c          266 g          592 t
ORIGIN      
        1 ttttacactc tcataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacacataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1381 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1441 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1501 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1561 ccatggataa taataagtct ctatttttca ataactattg caaaattata tcttaattaa
     1621 gaaaatattt ttattttaaa gataaaaaat tcttcatcct gcaatacttt atattctata
     1681 ttgtaattat tcagaatatt acttacaata tataaaccta atccattact attttcctta
     1741 tttaaatcaa atttaacatc aaatatttta tccatattcg aaatttt
//
LOCUS       DACDCJ010000189         1781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDCJ010000189 DACDCJ010000000
VERSION     DACDCJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.199"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(628..1512)
                     /locus_tag="IV038_002974"
     CDS             complement(628..1512)
                     /locus_tag="IV038_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HAP8343108.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            complement(1499..>1781)
                     /locus_tag="IV038_002975"
     CDS             complement(1499..>1781)
                     /locus_tag="IV038_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343109.1"
                     /translation="YRTNKELLEKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNV
                     EKFFYFNDSAYIAVKDSKRIPEATLEYLEPIKLSVFYSLYEKEIADEAV"
BASE COUNT          577 a          314 c          209 g          681 t
ORIGIN      
        1 ccaaattaga atacatcctg attcagtatt aaaaattatt tcagtttctg aaatatagta
       61 cttactttta acaagtttaa cattacctat ttttacgatc tcatctattt gatctcctac
      121 gttatacatt tttgtttctc ctttcttgat atatatatta tatcaccttg actacttgta
      181 gtcaacttgg aataaaagaa atagtctact tgtagtcatg ttttcaattg gaaaaaaatt
      241 tatttaaaga ttcttttaag gaaacgctta aacaatattt cttagctttt ttacttgcaa
      301 attcaggaaa gtgactgtat aatcatttat ataaattatg aaataaagac ttcgtggatt
      361 gccaaagatt tttcttacta cgaacagtct tacgagtcaa cgattgttgg tagctttcgt
      421 tggctcttta tttttgccac aaaagactac ttgtagtctt ttgaaatttc attttcaggc
      481 tacttgtagt caaaaaaaaa ggaaagacat tttcattgtc tttccttttt tttacgatct
      541 ttcgttagtc ttctgtacgg cccaatcgtt cttgtacttt cttttgaagg cgttcttctt
      601 tcagataatg tttttctgta cactatgtta gtgaacttca gcaaattcat tttctgcccc
      661 ctgttcacta agctgttttg gattccaaac tagttcataa tctccaaaga aatctttcaa
      721 aaccataggt tcattattaa atattgctcc tgtgtcgtac aggctctcct gaagagtttt
      781 gttataacta ccagttaaaa atacttcttt acttctatag atatatttat cccacttact
      841 atccatttta aataaaaaca gctcaacatt ttttaaattt ccataaggtg tttttaaaca
      901 atagctttcc ttttgaatat cagaaaagac ttgctttaca atatgtttaa acagcatttt
      961 ttcagctata ttactaacta aatataggag taaaaatgca ccaagtttag ttgtcgtatt
     1021 ataataattt tgaaaatgaa tttcatgctc atacaggcta agtagaaata aaactgtgcc
     1081 ctgtctacaa atgattttta ttcctttttg aaaaggcttc aaataattaa tataattgtc
     1141 atttgttgac atcattattt ttcctacttg taagataaat aaagcaaaaa taaacaggct
     1201 gaaagcaaga cttcctaaaa tatttaaggc ttctttcacc tctaatgatt cttttaatcg
     1261 atcagttccg gcgaacagtt tataaaagct ataagcattg ctccatccta ctctagcaat
     1321 gaatagccag accagataac ctaaacaact aaaactaatt ttcagccctc tcactatatc
     1381 taattgaaaa atgagtctaa attccggctc tctatacata cttttaaatt taacaatttc
     1441 ttttttcaat ccgagcgata atgactcttt gcctattttt ttactcattt tactcccctt
     1501 aaaccgcttc atcagcgatt tctttctcat aaagtgaata aaaaacagac aatttaattg
     1561 gctctaaata ttctaaagtt gcttcaggga ttcttttact gtctttaacc gcaatataag
     1621 ctgaatcatt aaaataaaaa aatttttcta cattactttt tgcactaaaa ccatcataaa
     1681 aaattccaaa aagttcttta ctacgttttt ttgaaaatgg attcaccaat tcaaaaacac
     1741 gctttgaaat tttttctagc aattctttat ttgttctata g
//
LOCUS       DACDCJ010000190         1640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDCJ010000190 DACDCJ010000000
VERSION     DACDCJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.200"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..152)
                     /locus_tag="IV038_002976"
     CDS             complement(<1..152)
                     /locus_tag="IV038_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP8343110.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVT"
     gene            complement(211..672)
                     /locus_tag="IV038_002977"
     CDS             complement(211..672)
                     /locus_tag="IV038_002977"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP8343111.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            complement(683..1459)
                     /locus_tag="IV038_002978"
     CDS             complement(683..1459)
                     /locus_tag="IV038_002978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8343112.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFN
                     INRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLI
                     GYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWL
                     FLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
BASE COUNT          585 a          312 c          188 g          555 t
ORIGIN      
        1 atagttacag tggctactac tccaaaagca tctggagtca ataatctagc taaaatcata
       61 ttcgaaattg gagttataat ctttgcaatt atttcagcta aggatgacca ttttaaagct
      121 tttgctgtct ttccatgaat attatttccc atttattacc ccattttgct aaataaatat
      181 agcaaccttt cgcatattaa acattacttt ttaatcaaat ttataatcaa atttcaactc
      241 tgttatgatt gctggatttc catatactaa gctatatggt ggaacacttt ttgtaactac
      301 agcatttgca cctatgattg aatcatgtcc gatattaatt ggtcctaaaa cacatgcacc
      361 agcgaaaaca gccacatttt cctctagcgt tggtgcccca acagtttctt ttccatttat
      421 tattttgcca tttccaccca aagtcacatt ttgatatatt ttacaattat taccgatttt
      481 tgttttaggg tggaatacac aaccaagacc attatggact agttgagcat tagagctaaa
      541 atctacctgt ccgtagaatt cacatccata aataatcctt aaaatatagc aggatatttt
      601 tgacatttta ttttgaccat tcctgtattg tctacaagct ttatctgccc atttctctgc
      661 ttttcttctc attttttcta cctcaacttt ctttttttat agttctttct gatgcagaaa
      721 tcatacacaa aacaactatt gcgtaaagaa aacgcgaaga tacaatatcc ggtataaaca
      781 ttgctcccac tactatcatc aaaaataacc atttatagca acctgatttc tttaattctt
      841 catacgtaaa tttaaagaag ccacttaaaa taattagcgt accaataatt cctgtctgta
      901 ataaatattg aataaaaaaa ttatgtggta ctgctacacc tagtagcgtg tataaattat
      961 ttaatcctaa tccatatcct attaagtatt tatcactaaa agcatttaaa gaggacatat
     1021 agttttctaa tctgcctgaa gaatccaaaa gactttgtcc agaacgacct gccattaaca
     1081 ttactagtat ataaggtgta gttatgattc cacctattag aactaaaaga tgaggaacac
     1141 taattctatt tatattaaat agataataca atgtcattgt gacaaacaag gaaactaacc
     1201 ctgttcttgc actaacacat aaaattgcac ctaataaaaa tacttctatt attataaaga
     1261 ataataattt ccaatttttt aaatccacat attttagaaa agcatataga cagcctgtag
     1321 caagatataa ccctgcaaaa ctaaaatctc ccatcaaacc tgcatatgca attctattta
     1381 aacccatttc tgcataatgg cctattatta caccagtaga taaaatatat actcgttgca
     1441 aatatacctg aaacgccata gatactactc ctatgaaata gaaattccac ccaatcatag
     1501 ttaagttttt gctatacatt ttaaaacgtt ttcctattaa aaaactacag ataaacaacg
     1561 aaatagtcat taattgcttt aatgcatcta ttaaaaaagg taagaacata gacgtcgata
     1621 ttccaaaaca aataaataaa
//
LOCUS       DACDCJ010000191         1625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDCJ010000191 DACDCJ010000000
VERSION     DACDCJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.201"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..376
                     /locus_tag="IV038_002979"
     CDS             <1..376
                     /locus_tag="IV038_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP8343113.1"
                     /translation="HSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNILKIE
                     ELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNINKL
                     EQTISSIGKKYTKKKMKGGDTI"
     gene            415..1557
                     /locus_tag="IV038_002980"
     CDS             415..1557
                     /locus_tag="IV038_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP8343114.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT          654 a          252 c          281 g          438 t
ORIGIN      
        1 acactctaat ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga
       61 atttattgct aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat
      121 cttaaaaata gaagaattat tcatcaatgc tattgtagaa aaatttgaca ataacattct
      181 tttcctcact agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat
      241 tcaattgaaa gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa
      301 caagctagaa caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg
      361 aggtgatacc atctagtggt aagtactcct tgtaaactaa gaggagatga tttaatggca
      421 aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt
      481 gccaccgatc cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa
      541 caagaagctc aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag
      601 aaagcacctg atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc
      661 agtgttaaag cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca
      721 gcatttggta agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat
      781 gaatggcaca gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta
      841 cgctacgggg ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca
      901 cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta
      961 ctggatgctt ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca
     1021 tacacaggta tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt
     1081 aataaagaac tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt
     1141 caatctccga aaacaagagc ttctagacga gttatctctt tagatactga gactctttct
     1201 atcctcaaca actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact
     1261 agctctaaag agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact
     1321 gttaatgatt ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat
     1381 gggtttagac atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt
     1441 caacaacgat tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca
     1501 aataatgcac ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag
     1561 agctggtcaa aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta
     1621 tatat
//
LOCUS       DACDCJ010000192         1567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDCJ010000192 DACDCJ010000000
VERSION     DACDCJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.202"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1567)
                     /locus_tag="IV038_002981"
     rRNA            complement(<1..>1567)
                     /locus_tag="IV038_002981"
                     /product="23S ribosomal RNA"
BASE COUNT          330 a          482 c          341 g          414 t
ORIGIN      
        1 tctctcaggg atcttacttt cttaaagaaa tgggaaatct catcttgagg tgggcttcac
       61 acttagatgc tttcagcgtt tatcccttcc ctacatagct acccagcaat gcccttggca
      121 gaacaactgg tacaccagcg gtaagtccat cccggtcctc tcgtactaag gacagctcct
      181 ctcaaatttc caacgcccgc gacggatagg gaccgaactg tctcacgacg ttctgaaccc
      241 agctcgcgtg ccgctttaat gggcgaacag cccaaccctt gggaccgact acagccccag
      301 gatgcgacga gccgacatcg aggtgccaaa cctccccgtc gatgtggact cttgggggag
      361 ataagcctgt tatccccagg gtagctttta tccgttgagc gatggccctt ccatgcggaa
      421 ccaccggatc actaagcccg actttcgtcc ctgctcgact tgttggtctc gcagtcaagc
      481 tcccttctgc ctttacactc ttcgaatgat ttccaaccat tctgagggaa cctttgggcg
      541 cctccgttac cttttaggag gcgaccgccc cagtcaaact gcccatctga cactgtctcc
      601 caccacgatt agtggtgcgg gttagagtgg ccataacgca ggggtagtat cccaccagcg
      661 cctccatcga aactagcgtt ccgatttcta cggctcctac ctatcctgta catgcggtac
      721 agacactcaa tatcaaacta cagtaaagct ccatggggtc tttccgtcct gtcgcgggta
      781 acctgcatct tcacaggtac taaaatttca ccgagtctct cgttgagaca gtgcccaaat
      841 cgttacgcct ttcgtgcggg tcggaactta cccgacaagg aatttcgcta ccttaggacc
      901 gttatagtta cggccgccgt ttactggggc ttcaattcgt accttcgctt gcgctaagca
      961 ctcctcttaa ccttccagca ccgggcaggc gtcagcccct atacttcatc ttacgatttt
     1021 gcagagacct gtgtttttga taaacagtcg cttgggccta ttcactgcgg ctgatcgtat
     1081 gatcagcacc ccttctcccg aagttacggg gtcattttgc cgagttcctt aacgagagtt
     1141 ctctcgctca ccttaggatt ctctcctcga ctacctgtgt cggtttgcgg tacgggccgc
     1201 tgttttctca ctagaagctt ttctcggcag tgtgacatca ggaacttcgg tactattatt
     1261 tccctcccca tcacagcttg tccttaagaa aagaagcatt tgactcctct tcagacttac
     1321 tgcttggaca gacatttccg atcgtctgca ttccttagcc tcctgcgtcc ctccattgct
     1381 caaacaaaaa caacgggtac aggaatatca acctgttatc catcgcctac gcctgtcggc
     1441 ctcggcttag gtcccgacta accctgggcg gacgagcctt ccccaggaaa ccttagtcat
     1501 tcggtggaca ggattctcac ctgtctttcg ctactcatac cggcattctc acttctaagc
     1561 gctccag
//
LOCUS       DACDCJ010000193         1517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDCJ010000193 DACDCJ010000000
VERSION     DACDCJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.203"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(606..962)
                     /locus_tag="IV038_002982"
     CDS             complement(606..962)
                     /locus_tag="IV038_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP8343115.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IV038_002983"
     CDS             complement(976..1386)
                     /locus_tag="IV038_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP8343116.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          466 a          274 c          216 g          561 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgcttttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctat
//
LOCUS       DACDCJ010000194         1456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDCJ010000194 DACDCJ010000000
VERSION     DACDCJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.204"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            76..1416
                     /locus_tag="IV038_002984"
     CDS             76..1416
                     /locus_tag="IV038_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8343117.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          462 a          322 c          284 g          388 t
ORIGIN      
        1 ctaataaacg caaacagaca ccttgaaatc ctttagaata aagtcgacga aaaccattca
       61 aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga tattctagac
      121 ctaaatctta cctttcatga agatgccttt cgcaaagagc gcattaatga cgaaacatgt
      181 tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg ccactatcaa
      241 aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt gaatgatgtc
      301 agtaactaca agaccattgt gcggattaac aaacagcgct tcaaatgtaa acactgtgga
      361 aagacttttt tagcagaaga ttcagttagc gatcgtcgct gctccattgc ccgcagagtc
      421 aaacaagcca ttcttgagct attaagtgag cctatttcta tgtctttgat tgcacgtatg
      481 aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc taaaaccgtt
      541 tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc ggtgaaaaac
      601 gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact gattgatatt
      661 gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc tagaaaagtc
      721 cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac attggtgaaa
      781 gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca acacatcggt
      841 cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg ggatcgtcgg
      901 tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga attgaatggt
      961 cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcgga gatagtcgat
     1021 cggctattat attatgatga ctccttaaag cgaggctacg aagtctacca ggtttttctt
     1081 tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga agactacaag
     1141 gagttgccag agcactatca accagtgttc acgaccttca agaaatatcg aacagaaatc
     1201 aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa caaccatatc
     1261 aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactaccg agaacgaatc
     1321 tttttatatc gaggaaaata tttcaagaaa actaagaaca ctacccaact gactaaagcc
     1381 agaacaacaa cacggctaga caagatagct gtctagccgt gtgagtatct cgattattct
     1441 attgaatttc ttcacc
//
LOCUS       DACDCJ010000195         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDCJ010000195 DACDCJ010000000
VERSION     DACDCJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.205"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            306..1354
                     /locus_tag="IV038_002985"
                     /pseudo
     CDS             306..1354
                     /locus_tag="IV038_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          542 a          170 c          275 g          430 t
ORIGIN      
        1 aggcttacca gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct
       61 tcgttgacgt cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta
      121 ttttttctgt ctagtctagt gtagccgatt caagttcttc gtgttctctt agacataaaa
      181 attcctcctg gtatgttttc ttctagtcta cacaccttaa tttttctgtc tagttaattg
      241 tagcctatcc agtttggaaa aataaaaaat tatatttcat caacgtttca taaaaggtgt
      301 gtagaatgtt attgaaagta gctataaaat gctattgtgt aaaaaataaa ggaggaataa
      361 tttatgaaaa aatagtacca agaggagcat gcctattact tgcattatgt ttgagcttaa
      421 gcgcagttgg gtgtcaaaaa agtaatgaaa acaataaaat aaaagaagga caatccataa
      481 gttctgaaga ggaaggcatg tcaattaatg caaatggcaa aaatgaaact tttaagccat
      541 cagattatac attggaggca aagaaagaat atgtttatga atatttaggg cttaaattta
      601 aactctcaga caaatttaga aattatatag ctgataaaaa gatagcgatg ttagatgatc
      661 aaagtcctat agataaagaa ctaaagtatg ctattttgac atttgagaaa atgacagaag
      721 aacaaaagaa tgcagttata gaaaagatgg gcgatggata taaaaattgg caaaatgagc
      781 ttgaaagaat tgggactatt ggtatatttg aaaaaaatac atcagaagaa aaaaatctaa
      841 aattataaaa tgtgatactc atactaaaat aggagtttca agtgatggaa aatacgattg
      901 ctatttaagc acaaatagcg gagctgaaag taatcttctt gatgaattaa aaagaactga
      961 gattcaaatt atagataaaa aagaacgtcc taaaaatggt tttgtgctat cagaaaaaac
     1021 tgatttagaa aatacagaag catttaacaa agaatcggtt aaagatttac gtaaactacc
     1081 aacaaaggac ataaatggca aggattttac aagtaaagat tttgagaagt atgatcttac
     1141 tatggtaaat gtatttgcaa cttggtgtac agcttgtgta aaggaaattc ctgatttggt
     1201 tgaggttcag aatgaaatga aaagcaaggg tgtaaatatc gttggagttg ttacagatcc
     1261 tgttgatgat aacggtgaaa acaaggaagc aattgaaaag tcaaagttaa tacatgaaaa
     1321 gacaaaggct tcatatcctt tcttaatgcc agatggaatg gcaacacttt ttttgacaaa
     1381 aattataagg tgttgtgaat gctgcggtgt tatggct
//
LOCUS       DACDCJ010000196         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDCJ010000196 DACDCJ010000000
VERSION     DACDCJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.206"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            84..1379
                     /locus_tag="IV038_002986"
     CDS             84..1379
                     /locus_tag="IV038_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP8343118.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          446 a          279 c          267 g          425 t
ORIGIN      
        1 cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat taatttggct
       61 accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat accctaaata
      121 ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa tacaaagggc
      181 aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt tcccattgtc
      241 tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg ttgttactga
      301 atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt aaatgtaagt
      361 cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt ttcattagtc
      421 aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg acggaaatcg
      481 ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga ttctaccgcc
      541 cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa ttcaaatcgg
      601 ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc catgcattat
      661 tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct cggttttcat
      721 tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct tatgtttcgt
      781 tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac atcgtccaac
      841 atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga cttaaaggaa
      901 attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa ctcctacaaa
      961 aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt aaagattatt
     1021 tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta cgccaaggct
     1081 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ctatttgagg
     1141 attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact ttacgaacgt
     1201 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aacggaccat
     1261 tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcaact
     1321 tttacaattt caaactacga attatgattc gtcatggcaa aacattttta accaaataaa
     1381 cagaacagaa agaggttttc ttctttctgt tctgtcc
//
LOCUS       DACDCJ010000197         1398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDCJ010000197 DACDCJ010000000
VERSION     DACDCJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.207"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..737
                     /locus_tag="IV038_002987"
     CDS             6..737
                     /locus_tag="IV038_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP8343119.1"
                     /translation="MLKIDGTITIKEIIDELDISDMTARRDLDALEADGLLTRTHGGA
                     QLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPGTTLEQLALELKG
                     RKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMASTNLKAIRFAKA
                     FVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYDFFNFYNLDQLDT
                     IITDNQISPQHLEEFSQYTTILKAD"
     gene            742..1398
                     /locus_tag="IV038_002988"
                     /pseudo
     CDS             742..1398
                     /locus_tag="IV038_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
BASE COUNT          502 a          247 c          235 g          414 t
ORIGIN      
        1 tagatatgtt aaagattgat gggaccataa ccataaaaga aataatagat gaactagata
       61 tttccgatat gacagcccgt agagaccttg atgctctaga agctgatgga cttttaacac
      121 gtactcatgg tggtgcacaa ttactttcct ctaaaaagcc acttgaaaag acacatatcg
      181 agaagaaaag tctaaataca aaagaaaaaa ttgacattgc taaaaaagcc tgctctttaa
      241 tcaaagatgg cgatactatt tttattggac ccggaactac acttgaacaa ctggcattag
      301 aattgaaagg tcgtaaaggt tataaaattc gtgtcattac aaatagtctc cctgtgttct
      361 tgattctaaa tgatagcgaa accattgatt tattgcttct tggcggtgaa tatagagaaa
      421 taactggagc ttttgtaggt tcaatggctt cgacaaattt aaaagcaatt agatttgcca
      481 aagcttttgt tagtgcaaat gctgttaccc ataattctat tgctacatat agtgacaagg
      541 aaggtgtgat tcaacaactt gccctaaaca atgctgtaga aaaattctta ttagtagaca
      601 gtactaaatt cgatcgatac gatttcttta acttctataa tctagatcaa ctcgatacca
      661 tcattacaga taaccagatt agccctcaac acttagagga atttagccag tacactacta
      721 ttttaaaagc ggactagaat tatgacttat aaaaatattg tttttgacct tgatgacact
      781 ctttatgacc atcaactccc ctttaaaagt agcgttgaga aatgttttcc aacgattgat
      841 atccaacaaa tagataacat ttacaaacgt ttcagatgtt ggtctgatgt tgcttttcct
      901 aaatacacga aaaactcatc agtattgagc agcttagaat ttttcgctgt cagaagacta
      961 tggaagaatt tggcatcgat agtatttcta ggacagaagc tctagacttc caagctgact
     1021 atgaatatga actcgatcag attacgatga ttcctgagat acatcaacta ctactggctc
     1081 tacataaaaa ccgcatccca ataggtattc taactaatgg ccctgttgac ttacagagtc
     1141 gaaaactcca aaatattggt gcctatcaat attttgaaaa gcaaaatatc atcattagtc
     1201 agtccactca cggaggagct gcagaaaata gctatgaagg taatgtaaaa aacttacaaa
     1261 gttaccaaaa tgtccaatta tctaaaaggg ctatagaaat tcttaaagag gaaattgagt
     1321 taaatcacca acatataaga ttcaatcctg attataaaga taatggatgg atttttactt
     1381 ccaaaagtcg tcataaac
//
LOCUS       DACDCJ010000198         1391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDCJ010000198 DACDCJ010000000
VERSION     DACDCJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.208"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..334
                     /locus_tag="IV038_002989"
     CDS             <1..334
                     /locus_tag="IV038_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343120.1"
                     /translation="NEPTTVVDNNQELTPLDRDGIAIGNPTNDLTVTENYLIVSPLLA
                     LAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNWIAT
                     YSGTLTRK"
     gene            344..547
                     /locus_tag="IV038_002990"
     CDS             344..547
                     /locus_tag="IV038_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343121.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            758..1342
                     /locus_tag="IV038_002991"
                     /pseudo
     CDS             758..1342
                     /locus_tag="IV038_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          496 a          206 c          261 g          428 t
ORIGIN      
        1 aaatgaacca actactgtag tagataataa ccaagaatta actcctttag atagggacgg
       61 aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt taatagtttc
      121 tccattactt gctctagcag cttctaaaac gatacgagtg acaggaacga tgatttttga
      181 taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa aaagaaatgg
      241 ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa acaattggat
      301 tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag aatttgtgaa
      361 cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac cgaaagaaga
      421 atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa aatcaatttc
      481 taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat atcaaaaaga
      541 aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta attgaaagtg
      601 ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc aaaaaaacaa
      661 ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta tatggataaa
      721 gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat acgcccgggt
      781 atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat ttggttgtga
      841 aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat tagaaatggc
      901 cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc gtttaggtag
      961 aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg tgagttttca
     1021 tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc tcatgtttca
     1081 tttgtttgcg gcatttgcat aatttgagcg taatttgatc ctggaacgat ccgcagcagg
     1141 acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat tgtctgaaca
     1201 atatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga ctattgcgga
     1261 acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg aggagaaaaa
     1321 tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt gccatttatc
     1381 tagaaaagtg a
//
LOCUS       DACDCJ010000199         1315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDCJ010000199 DACDCJ010000000
VERSION     DACDCJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.209"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            53..814
                     /locus_tag="IV038_002992"
     CDS             53..814
                     /locus_tag="IV038_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAP8343122.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1042..1236
                     /locus_tag="IV038_002993"
     CDS             1042..1236
                     /locus_tag="IV038_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343123.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          419 a          207 c          250 g          439 t
ORIGIN      
        1 cactatattt tatcacatga aaattcatta agaaagtagg aatcgaagta ttatgcacac
       61 accagaagaa attatggaac tcagtattca tcttggaaca aaaaaaatac ataaacctat
      121 cttagctaaa ctgattttag gttttatcgg aggagccatg atttctctag gttatttggc
      181 atacgtacga gtctctgctt ctatcccaga atctatggct agcttgacta gtttagttgg
      241 tgcctcggtt tttcctatcg gcctgctggt catccttctt ggcggtggtg aattgatcac
      301 cggaaatatg atggctgtgg cgattgcctt tatggataaa aaagtcacat tctcacagct
      361 ggttaagaat tggtcagtga ttacttttgc aaatattatt ggtgcaattt tcgttgccta
      421 tttctttgga cactttgttg gtttaactca ttcaggtgtc tatctcgacc aagtgattca
      481 tattgctgat cataaaatcg ctgtttcttg gtggcaagca ttaggctccg gaataggttg
      541 taattggttt gtcggtttag cgttatggtt atcatatgga gcaaaagatg cggccggcaa
      601 agtactaacg atttggtttc cagtcatgat ttttgtggca atcggtttcc agcatagtgt
      661 ggccaactgt tttgtgatac ctgctgccat atttgaaggt caagcgactt ggttagaatt
      721 tctctcgaat tttatccccg tttatattgg aaatatcatc ggcggagcta tttttgtttc
      781 aggtttttat tacgttagtt ataagcatca ttaagattga aggtaatgtt cattcgagca
      841 ttaccttcaa tctctttata tactatttat attttaagaa caatagcctt ttattatata
      901 tattattcaa attagttata tcgagtgaaa aaaactttca aattgattct ttttatttat
      961 cataaagata tagatacata atcattttca cttacatata tttgaatatt aactaaaaac
     1021 agcaagaaag aagaggatca aatgtattat gtagaagtaa aaacaaaagg cgtcaaaaac
     1081 aaacaatatg tgaaaggtat cagtaacgaa tatcctttac ttggttcatg gaaagaagct
     1141 gctccttttt ccaaaccatg tgccataaaa ataaagaacg agctagaaaa agaactgact
     1201 tgtggcaaag ctgttgtaga tatcatcgaa aagtaaatcc aatgtataga aaggaagatc
     1261 acacatgaaa agcgacgtac aaattgctaa gattatagaa ttagaagaag aagac
//
LOCUS       DACDCJ010000200         1301 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDCJ010000200 DACDCJ010000000
VERSION     DACDCJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.210"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..297)
                     /locus_tag="IV038_002994"
     CDS             complement(<1..297)
                     /locus_tag="IV038_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343124.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSE"
     gene            complement(442..>1301)
                     /locus_tag="IV038_002995"
     CDS             complement(442..>1301)
                     /locus_tag="IV038_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP8343125.1"
                     /translation="QNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT          449 a          226 c          141 g          485 t
ORIGIN      
        1 ttcactcaaa tacttataat ctattaactt gtagttgttt aacttttctg aaaaagttag
       61 acccacgtaa taatcttccc agctttctct ttcaattaac tgatccaatt tttgataatc
      121 cataaattca tcatgtatta aaaagaataa ataacttgat attttagcat ctagcttaat
      181 cagattaaat aatgtttgct taaaattgct accattaata tgactgatag attctctagc
      241 ttcttcacat aaacttttta ctttattaat tactactttg tcatattcat ttaacacact
      301 attattacca ttacttagac gctcagattc tacgaaacaa ctcaaatcag aaatttccca
      361 aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa atgatatagt
      421 ctcataataa aataaaacct ctcagctaat gttgttgaaa ttatccacta aacctaattg
      481 atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt gtgaagaatc
      541 tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca acattatttt
      601 agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca tttcattctt
      661 aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt cctttacagt
      721 aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta agggagaaag
      781 atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag aatccctcac
      841 ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag catctctact
      901 ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta acttttcaaa
      961 aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac ttggaattat
     1021 tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat ttttaacata
     1081 ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat tttgtgataa
     1141 cagattatct attttgattt gctcgatagt tttacttaga tttcctgtat attcaattaa
     1201 cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta aaatcagaat
     1261 atctcgaatg tctcctaaag aatacaaagt caaattttgc a
//
LOCUS       DACDCJ010000201         1289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDCJ010000201 DACDCJ010000000
VERSION     DACDCJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.211"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(15..1154)
                     /locus_tag="IV038_002996"
     CDS             complement(15..1154)
                     /locus_tag="IV038_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP8343126.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          336 a          281 c          239 g          433 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttatt
//
LOCUS       DACDCJ010000202         1278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDCJ010000202 DACDCJ010000000
VERSION     DACDCJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.212"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..240
                     /locus_tag="IV038_002997"
     CDS             <1..240
                     /locus_tag="IV038_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8343127.1"
                     /translation="RQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLIL
                     DIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            245..1192
                     /locus_tag="IV038_002998"
     CDS             245..1192
                     /locus_tag="IV038_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP8343128.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          419 a          184 c          267 g          408 t
ORIGIN      
        1 aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa
       61 tttctaaaaa aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat
      121 tatctaattt tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct
      181 aaatttttag aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa
      241 aaggatgagc tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa
      301 tcatttagga gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga
      361 aaatcttgat atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt
      421 gatggaagag atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc
      481 tagtgtggca gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt
      541 attaatgatt ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag
      601 ttcttcagcg gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat
      661 tcgcccattg acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta
      721 ttgtcattta tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa
      781 tcaaaatccg aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa
      841 acaaatcgta ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga
      901 ctttgtttat atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc
      961 actggggaaa caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca
     1021 tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg
     1081 tgatatccat gactctctag cagatattac taagatccag tcaattggct atcaaccgaa
     1141 atatggtatt ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact
     1201 aagataagat tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa
     1261 aatcaaatct gggataga
//
LOCUS       DACDCJ010000203         1255 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDCJ010000203 DACDCJ010000000
VERSION     DACDCJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.213"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(335..889)
                     /locus_tag="IV038_002999"
     CDS             complement(335..889)
                     /locus_tag="IV038_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP8343129.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          406 a          218 c          132 g          499 t
ORIGIN      
        1 atttagccca taatctaata caacttcttc atactcatat ttctttaaat atttaatatg
       61 aaaatatgct gaattaatca aaactcgagt gttactgttg ttatttattg ttgttctatt
      121 gtgtataaaa ttgtataggt tgtcatattt tcttggttta ttaaaatcat tctctatata
      181 ataatcatta tcaactacaa cattttgttc tatctctggt attctattgt aaatgttact
      241 aaacgtttta aatacttccg aaaattcagt tatcttcatt aatctcacct tctaagtttt
      301 cattatacac aaaaaatata tcctaatatc tcaattagcc tcccaattta aaaccacgta
      361 acgatgcccc tatttcatct tcagttatac caatgtacct ttttgtaatt gcttgcgagc
      421 tatgattaaa aataaacatt aaggtagcaa tgtctctagt tttcttgtag tagtgatacc
      481 caaaagtttt tcttaatgta tgtgttccta tatcatctct tcctaatttt tctgctatat
      541 ctgcatatat ctgatagaca cgatcccttg ttatatgtcg accttgttgc cgacttggaa
      601 ataaccaatc attttcttct ttaccttcag tatataataa aatctcttct ctcaaattat
      661 ctataaaaat cggttttcgc ttacctgttt tatgttcaac aataataggc ttacttttac
      721 ctcgtatgtc tccaactttt aatcctataa tatctgacat acgtaaccca ttattgattc
      781 caaacaaaaa taaaaaacta tctctttctg gattacttgt tcttcttagc caaaactgaa
      841 aatcttgtat ctcttcattt gtccttaacg gttgtacatt atagctcact attgtttccc
      901 ttcttaaaaa tattctgagt tcatgattat atatatattt taccataatc atgaacttat
      961 atatatactc tgttaaaagt gaagaaaatc aaggggttta gtttatgatt ttatatcatt
     1021 tcagtaactc agaatattcc gacttttttg tacaaaaaaa ttaaccttac ttatatgaag
     1081 attctaagct cttatttaaa ttttagacgg gcagttatcc gtagaagttc ttagaatagc
     1141 taatttacat aattaaatta tatgaaattt aagtttgaac aatatactaa gaactcccct
     1201 tactataaag tgaaataaat tagcatatga ataaccgtgc taccaaactt ttcat
//
LOCUS       DACDCJ010000204         1195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDCJ010000204 DACDCJ010000000
VERSION     DACDCJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.214"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IV038_003000"
     CDS             <1..1076
                     /locus_tag="IV038_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP8343130.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACDCJ010000205         1189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDCJ010000205 DACDCJ010000000
VERSION     DACDCJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.215"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            16..1101
                     /gene="xerS"
                     /locus_tag="IV038_003001"
     CDS             16..1101
                     /gene="xerS"
                     /locus_tag="IV038_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP8343131.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          480 a          195 c          185 g          329 t
ORIGIN      
        1 gaaaggatga ataacatgaa aaatacagaa atatatcaaa aaatagatac aatcatactt
       61 catatgcccg attatataaa agattttgtt caagatcgtg aggataaaga tcaatcacct
      121 cgaactactt tagaatatct aaaaaattat aagctctttt atgaatggct tctgagtgag
      181 tctatcgttc ccgatacttc aatcacctcg ataagacagt taactgccta tgatctaaac
      241 ttgtacaaat ctcatttgaa acgtcgcaca aaggaaaatg taaaaaaaga gacaacaaaa
      301 actaagctca aagataataa taacttaggt ctatctactt ctactatcaa tagaaatatc
      361 acagcattaa aggttctatt taaatatctt tcgaaaagtt ccaataatcc tttaggaaaa
      421 ccatacttag aaaataatcc tatggatcaa gtagcaacta tcacagataa aactacgtta
      481 gctgcccgtg caaacgcaat cgagaaaaaa ttatttctag acgaagatac acaaaattat
      541 ctagattata ttgccaatga atataaaaaa actttgagca aacgagcttt gatctattat
      601 caacgagatg tagaaagaga cctggcgatc aatgcattga ttttaggatc tggattaaga
      661 ctctccgagg ttgtaaatat taatttagag gacctatctt tagaaaagaa taatgtggta
      721 gtaactagaa aaggaaataa gagagacgca gtaaatatag cagcatttgc aatggaatat
      781 ttagctaatt atctagctat tcggaaagaa cgatacaaag taacagaaaa cgaaaaagct
      841 ttatttctag caatttatca aggagaagcc aagcgtatat ccggtattgc aatcgaacgg
      901 atggtagcaa aatattcaaa aggattcaga gtccaagtca gtccacataa attacgtcat
      961 actttggcta ctagactcta tcaacagaca aattcattgg tattaactgc ccaacaattg
     1021 ggacatacaa gtacaaatac aacaacttta tatactcata tagacaatgc agctacaatt
     1081 gatgctttga acgctttgta aatgtagtaa aaaatagtat aataatgaag cctgtctcta
     1141 aaattagata aaagcccaac tttaaaggca agcttcatta tcattttta
//
LOCUS       DACDCJ010000206         1187 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDCJ010000206 DACDCJ010000000
VERSION     DACDCJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.216"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            44..304
                     /locus_tag="IV038_003002"
     CDS             44..304
                     /locus_tag="IV038_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343132.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(666..905)
                     /locus_tag="IV038_003003"
     CDS             complement(666..905)
                     /locus_tag="IV038_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343133.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          421 a          165 c          219 g          382 t
ORIGIN      
        1 ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata acgaaactat
       61 cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag tggttggaga
      121 agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc atgacgtcga
      181 tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc acgtttatga
      241 gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata ctttagtttc
      301 ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga aggcacttta
      361 tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc ctagagaatc
      421 tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc cagaactgtc
      481 ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa tcatttcaaa
      541 aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa gcatttgtaa
      601 aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg aagaagatga
      661 agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt ctttaccctg
      721 ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt tttctcattt
      781 gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat gttctttgat
      841 gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa cagaattctt
      901 ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc agtattaggc
      961 aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt aatacatata
     1021 tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt attaacgatt
     1081 atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg tatgatatta
     1141 tctagaggta atttttttgg gtatttaaat agttgctagg aggatag
//
LOCUS       DACDCJ010000207         1147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDCJ010000207 DACDCJ010000000
VERSION     DACDCJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.217"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..357
                     /locus_tag="IV038_003004"
     CDS             <1..357
                     /locus_tag="IV038_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAP8343134.1"
                     /translation="LNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGD
                     LGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIK
                     PDWEKIKYYILLDELF"
     gene            complement(465..653)
                     /locus_tag="IV038_003005"
                     /pseudo
     CDS             complement(465..653)
                     /locus_tag="IV038_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            633..>1147
                     /locus_tag="IV038_003006"
     CDS             633..>1147
                     /locus_tag="IV038_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP8343135.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT          378 a          183 c          269 g          317 t
ORIGIN      
        1 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
       61 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
      121 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
      181 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
      241 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
      301 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
      361 ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg
      421 aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga
      481 tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt
      541 ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac
      601 tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta
      661 ttcttattca gctgcaatca aaaaaaataa ttcctgccaa agaaattgca cagcgattta
      721 atataagctt aaggacagtt tacagagata tccggacact tgaagaagcc ggaataccaa
      781 ttggatctga ggccggaaaa ggatattttc ttgtagaggg cttcctactt ccgccggtaa
      841 tgtttacagc ggcggaagtg ggtgcattga ttacagcagg gaaattttta aattgccatg
      901 gagatgaatc atttataaag gattttgatt cagctatgta taaaatcaaa tctattttga
      961 agcatggtga aaaaaactat gcacaggagt tggagaacag tattaacgtg tacagcacat
     1021 ctggacagaa aaatacattg gcggataacg ttattgcagc aattcagact gcaatctgca
     1081 ataaaagggt aatatcaatt caatatcctg catcaggagg gcaagaaccg gaaagcagaa
     1141 tgataga
//
LOCUS       DACDCJ010000208         1102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDCJ010000208 DACDCJ010000000
VERSION     DACDCJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.218"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1102)
                     /locus_tag="IV038_003007"
     CDS             complement(<1..>1102)
                     /locus_tag="IV038_003007"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP8343136.1"
                     /translation="EFDNEEHMFSSTNVAFTSNLGISLLLWLFICVFNNKIAHLVGNS
                     GLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALIPLVITVPLAIIGL
                     SYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSAWSLVESLGTWLSS
                     YIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTLSRLQNSKTEYDKT
                     YLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIYGLMSCFTLVLGQY
                     ASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRSFVKIEQIIVFWII
                     LWFGFKFNPFKILKQVWKSCASALLMGVLAKI"
BASE COUNT          418 a          197 c          137 g          350 t
ORIGIN      
        1 ataatttttg ctagaactcc cattagcaat gcactagcac aagatttcca aacctgcttt
       61 aaaattttaa atggattaaa cttaaaacca aaccataaaa taatccaaaa gactataatc
      121 tgctctatct taacgaaaga tctaccatat gctagttcta taaacccttt ccttgaatag
      181 attattagaa ctggaattaa tactgctaaa tgtaggatag tcataagaac atttgctttt
      241 ggttttccta aaccacgata gtactcagag gcatattgtc ccaagacaag agtaaaacag
      301 ctcattaatc catatatccc aacaaatggt acagcctctt tccattgact cccgagtaga
      361 atatttgtta ctagttccct atatacatat atacctatac ccagaggaat aataaaaatg
      421 gaaacaattt taataaaatc taaatatgtc ttatcatact ccgttttact attttgtaaa
      481 cgagataatg ttgaaaataa tacagttgtt gttgctgaag ttacaattgc aaaaatacca
      541 ttaaccgttg tcattgtagt tttgtatatt cccaaatagt atacgcttaa aatgcctcct
      601 acgataaaag tacctatata agatgacaac catgtaccta atgattccac tagtgaccac
      661 gcactaaagg aaaacatttc gtaaaattct ttttttgaaa aaaaaagttt tggtttccac
      721 tctgataact tagttaatat tgcagctgta actaaatttc ctattataga tccaattact
      781 agtgaccaat aacttagtcc aattatcgcc aacggtactg taataactaa aggtatcaat
      841 gcagatatta ttctagcata acacaaaatt ttgtaatcaa aatttcgtct aaaaattgcc
      901 gattgtatac tggaaaaaga agtaattgga agagaaactc cagctactat aattactaat
      961 ccaagtccag aatttccaac taagtgcgct attttattat taaacacaca aataaataac
     1021 cataataata aagaaatacc taagttacta gtaaaagcta catttgttga tgaaaacatg
     1081 tgttcttcat tatcaaattc at
//
LOCUS       DACDCJ010000209         1091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDCJ010000209 DACDCJ010000000
VERSION     DACDCJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.219"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IV038_003008"
     CDS             6..980
                     /locus_tag="IV038_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP8343137.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACDCJ010000210         1085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDCJ010000210 DACDCJ010000000
VERSION     DACDCJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.220"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..>1085
                     /locus_tag="IV038_003009"
     CDS             80..>1085
                     /locus_tag="IV038_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP8343138.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     P"
BASE COUNT          365 a          208 c          212 g          300 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccct
//
LOCUS       DACDCJ010000211         1083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDCJ010000211 DACDCJ010000000
VERSION     DACDCJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.221"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            80..>1083
                     /locus_tag="IV038_003010"
     CDS             80..>1083
                     /locus_tag="IV038_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP8343139.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     P"
BASE COUNT          363 a          199 c          212 g          309 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcacgacaa aataaacaac
       61 tgagaggacg atatgctata tgaatgattc tatcaaaaaa atgctgcgat taatagagaa
      121 agatttgatg attacagagg tctcttacga gacctttcag aagaaaaaga cgttgatcgt
      181 cgatgctgtt ttctcacctg ctccccatac ctgtagaaat tgtggttcta ctgtggtaga
      241 tggaaacggg aaagtaattg tagtgaaaaa tgggaagaag gaaacgattg tccgctttga
      301 acaatacaat cacatgcctt tggttatgcg cttaaaaaag cagcgctata cctgtaaaaa
      361 ctgtcgaacc cattggacgg ctcaaagtta ttttgttcaa cccagacatt caatcgcgaa
      421 tcatgtcaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaaattgt caggtatctt tgaccaccgt tattcgtacg ttgaaagagt ttaaaagcta
      541 cttaccaaag caatctaaga ggattctccc cagagtattg atggttgatg aatttcgttc
      601 gcacgcttcc atagaagata aaatgagctt tatttgcgca gatggcgaaa caggacaatt
      661 aattgatgtc ttgcctacgc gtaaattgcc tcgattaaca agctatttct taggctgcgc
      721 taatcctgaa gaagtaaaat ttttagtcac agacatgaac gccgcctact tccagcttac
      781 caaacgtatt ctgccaaatg cgaaagtagt gattgatcgg tttcatatta tcaaacacat
      841 gaatcaagcc tttaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagtaggaca
      901 gaaaagtcag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagttttcga gcaccaaaat acccatttct
     1021 tacggaagcg atgatgattg atcgattgct tggcttttct acgtccctga aggaggcgta
     1081 tcc
//
LOCUS       DACDCJ010000212         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDCJ010000212 DACDCJ010000000
VERSION     DACDCJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.222"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            2..>1073
                     /locus_tag="IV038_003011"
     rRNA            2..>1073
                     /locus_tag="IV038_003011"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACDCJ010000213         1048 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDCJ010000213 DACDCJ010000000
VERSION     DACDCJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.223"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..299)
                     /locus_tag="IV038_003012"
     CDS             complement(<1..299)
                     /locus_tag="IV038_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8343140.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIP"
     gene            complement(338..>1048)
                     /locus_tag="IV038_003013"
     CDS             complement(338..>1048)
                     /locus_tag="IV038_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8343141.1"
                     /translation="EMPFQTSAKITIENLHPKDMSSFFYTINYALVDELEENSLYFHA
                     NWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPT
                     VTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAV
                     HAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGNDDVRLFERQDDVASVAYWYST
                     EASGNTKRHFTDRERLPR"
BASE COUNT          304 a          234 c          200 g          310 t
ORIGIN      
        1 ggaatattga aatattgctt tagcgttcct acaaaattga aattttgcca agcaagtttt
       61 ggtgttgatg tgatatgacc gaaactcgtt gatttgcatt gaatatcaat tggaccaaag
      121 cagccgaccc cgatgcctgc caattgttcc ttaaaaggtt taaaaaaatc aatgacctgc
      181 ttcattgttt caactggagt tgttgtggga aaactgactc tttccaaaat cgttaattcc
      241 tcagtaccga ctgcacagac aaatttagtc cctccagctt caattgcccc atatatcatc
      301 ataggttcct cccttcgaat atgtgaaaaa cacttcatta acgcggcaag cgttctcgat
      361 cagtgaaatg acgtttagta tttcctgaag cttctgttga ataccagtaa gcgacactag
      421 caacatcgtc ttgccgctca aataagcgaa catcatcgtt gccgatttgt tgtaaagtga
      481 ccgataaatc ttcggtaaaa cagatggggt cttgaagatg ccaacgataa agaccatgtc
      541 gtggcattgc gtcgtcacca aaggcatgaa cggcattcaa ttttcccgtt tcaaaacgat
      601 ctcttgtggc atctctattt gattggtaag gataccctaa aaaggctgtt gaatagtttt
      661 tcgcatgtgg acgtccgaac tcatcgtact catgaaaggc ccacgccccg ccaaaataat
      721 cttctgaacc tgttgaggtt acggtcggat actcactatc cccatcaatg tagaatttaa
      781 actcgccttc gccccaccaa taacgttcta ggcaggtaag cgcaaggtaa gtacccacgt
      841 aatagccttg accctttaat cgatcaatta acttataatc ctcctgtaat ttggtaatgc
      901 gttcccgatg ccaatttgcg tgaaaatata ggctattttc ttccaattcg tccaccaaag
      961 cgtaatttat cgtataaaaa aatgaggaca tatccttcgg atgtaaattc tcgatcgtaa
     1021 ttttagcgga tgtttggaaa ggcatttc
//
LOCUS       DACDCJ010000214         1001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDCJ010000214 DACDCJ010000000
VERSION     DACDCJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.224"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1001)
                     /locus_tag="IV038_003014"
     rRNA            complement(<1..>1001)
                     /locus_tag="IV038_003014"
                     /product="16S ribosomal RNA"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DACDCJ010000215         1000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDCJ010000215 DACDCJ010000000
VERSION     DACDCJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.225"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            369..1000
                     /locus_tag="IV038_003015"
                     /pseudo
     CDS             369..1000
                     /locus_tag="IV038_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          343 a          181 c          182 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccactact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DACDCJ010000216          956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDCJ010000216 DACDCJ010000000
VERSION     DACDCJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.226"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(852..>956)
                     /locus_tag="IV038_003016"
     CDS             complement(852..>956)
                     /locus_tag="IV038_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8343142.1"
                     /translation="MEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          323 a          203 c          156 g          274 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acccacaatt tcatttgcca taaaatcctc
       61 cttcaagatt aactacttaa caaaattata aagagctttc taataggggt caagccccta
      121 attaaagacc cctaaaaaca gacaaaaaga cccccaaaaa cagacaaaaa gacccctaaa
      181 aacagacaaa aagaccccca aaagattctg cacaccttgg gagagtaagc atacagccga
      241 cggtaaagag gtttaaaaag aggtttaaaa agagattata aaagaggagg caaaatttcc
      301 cgcctaaaaa actcctgctt ttttcctcaa acaagttcgc gaatttttct aaactgggaa
      361 actcgctccc tacggtcact aaaaatgtgt gatcgctacg ctctcacatt atactgcgca
      421 gtcgcctaaa ggctcctttg cttaaaaaca gttcataaag aactcctttg aaaagttctg
      481 agcgtttgtt taagccgttt taagacagtc ttagtataaa acccttatct tgttctcaaa
      541 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aatgttctat aagacctcta
      601 aaattagatc taaggcgttt taaagttgtt ttggtataat tatataccag aacgtttaaa
      661 acgccttaaa acgcaaatta tagccatatg tatttttacg gaagccggca cactcatttg
      721 caaagcttca gctttgcaaa ttcggattcg ctcactcgaa aattgatttg ttttgactct
      781 gatcgatagg tgtatttggt tctgttgcaa agttttaaat aaagaataaa atcccttacg
      841 gtatctatga tttaagctgg gattcccaat aataccttga tttcagtaca gaccgaaaac
      901 ccgaagagag tgccttcttt tcgggttttc ttatataatc ctcggatggc ttccat
//
LOCUS       DACDCJ010000217          954 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDCJ010000217 DACDCJ010000000
VERSION     DACDCJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.227"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..819
                     /locus_tag="IV038_003017"
     CDS             <1..819
                     /locus_tag="IV038_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP8343143.1"
                     /translation="GTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGS
                     KEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKN
                     GNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSK
                     QNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          379 a          133 c          202 g          240 t
ORIGIN      
        1 ggtacgattg ataccaacac accaggtaat tacgaagggg ttatcgaagt aacgtatcca
       61 gatggtacga aagatacagt gaaagttcca gtagaagtga cagacaatcg ttccgatgcc
      121 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagacgca
      181 tctgagggaa ttaagaacaa agaagatcta ccaaaaggaa ctacttatac ttggaaagag
      241 aaagtagatg ttagtacagc tggaaacaaa aaagggattg tagtagtaac atatccagat
      301 ggatctaaag aagaagtaga ggttactatt tctgtagaag ataaaaaagc accgaataaa
      361 cctcaagttg atcctattac agagggtgac caaattgtta ctggtaaaac tgaaccaaat
      421 gcagaggtga cagttacatt accaaacggg agtcaatacc atgggacagc tgataaaaat
      481 ggtaatttta cagttaaagt tcctaaatta gaggcaggaa caaaagtgat agtaactgca
      541 actgatgaat ctggtaatac tagtgaacct actaatgtag ttgtttcttc aaacgaaaaa
      601 gacagtgaaa aagccgttag taaagataat aagactgaca atcaaggtag caagcaaaac
      661 aaaaatagag gcaaaagcag tcctcaaaag cagtcctcaa aagcttatcc taagacggga
      721 gagattgata gtaatatctt tactattagt ggtggtttaa tcctgttagg aactttaggg
      781 ttattagggt ataaaaatcg aaaaaaagag aatgaataga aaatcaattt cttaaatctt
      841 ctctgaaaag aggaagaatc tttttcttcc tctttttgtt ttttattttg ggagataggt
      901 tgttagttaa aaatgaagat acttttcata gaagggaaaa gaatgtgatt aagt
//
LOCUS       DACDCJ010000218          924 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDCJ010000218 DACDCJ010000000
VERSION     DACDCJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.228"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(24..176)
                     /locus_tag="IV038_003018"
     CDS             complement(24..176)
                     /locus_tag="IV038_003018"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343144.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(367..>924)
                     /locus_tag="IV038_003019"
     CDS             complement(367..>924)
                     /locus_tag="IV038_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP8343145.1"
                     /translation="NAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSH
                     PEMGHMLIKRHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEA
                     FYLAQCAYNTTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIIT
                     PELGDNAGIIGGLALARKAVRNKQP"
BASE COUNT          296 a          211 c          139 g          278 t
ORIGIN      
        1 ttttatacgg ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc aaggattatg
       61 aagtcaacat ttcgaaaaat gaacatacat tggtaagcaa ctataactat taccacagac
      121 ataaagtaac acaatatatt actatttaag tgtacgccac cagtgaatat cctcaaggta
      181 tacagcgcac aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca gtgatttaat
      241 gaaaacaaca cgatttgact tagagccttt ttatttgaat tcattttcgc tttatccaac
      301 ttcatttgat catttgatta ctcggttaaa tacttttgac atgagtatca atgaaacatt
      361 cattaattat ggttgcttat ttcttaccgc ttttctggcc aatgctaatc cgccgattat
      421 tccggcatta tcacctaatt cgggcgtaat aatgtagcta tcaattggtg gtatttcaac
      481 atatccattt ataagctcaa caaatttatc ctgaactttt ttcttcatgt gttcttgttt
      541 cattacacca ccgccaaaaa taattctatc tggcgaaaac atcagtgttg tattgtatgc
      601 acattgggca aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg
      661 gccttttaca cccaaccgtt tttcaatcgc tggtcctgct gccatcccct ctaaacaatc
      721 ttggtggaag ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg
      781 ccccatttcc ggatgactga acccttccac gaaagtccca ttgaacaagg caccacctcc
      841 gatacctgtt cccacagtga agtatgcaac acctgctaac cctttgccag aaccagcaac
      901 atattcacca tagcaagccg catt
//
LOCUS       DACDCJ010000219          912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDCJ010000219 DACDCJ010000000
VERSION     DACDCJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.229"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..912
                     /locus_tag="IV038_003020"
                     /pseudo
     CDS             1..912
                     /locus_tag="IV038_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          336 a          155 c          174 g          247 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg ag
//
LOCUS       DACDCJ010000220          909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDCJ010000220 DACDCJ010000000
VERSION     DACDCJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.230"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..527
                     /locus_tag="IV038_003021"
     CDS             <1..527
                     /locus_tag="IV038_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8343146.1"
                     /translation="FSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNHQAF
                     VTRLEELICLADLDGSWWWWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQYFGL
                     KFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILH
                     VEKGKTIYIQRRKR"
     gene            524..>909
                     /locus_tag="IV038_003022"
     CDS             524..>909
                     /locus_tag="IV038_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP8343147.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPT"
BASE COUNT          270 a          154 c          221 g          264 t
ORIGIN      
        1 ccttttctgc tagttctttc aatccaactt atagcgagga aatcaaaggc atcacttggg
       61 gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagt gacatgcaca
      121 accatcaagc cttcgtaacc cgattagagg agctgatttg tttggcagat ttagacggtt
      181 catggtggtg gtggccgtat cgtttagagg ctaaacaagg agaagttgtg cgagactttg
      241 gctgcggcaa atgcggctgg gcatcgggcc tttttgtcag tataatgatc agtcaatatt
      301 tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt
      361 cttggaaaaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt
      421 tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc
      481 acgtagaaaa gggaaaaacg atttacattc aaaggagaaa aagatgagta tactaaaaaa
      541 tattacccaa tttaaaaaaa tccagagtcg agcaatcact cccgaaaatc caacaggcga
      601 gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca agccgcaaag gtcgtggcgc
      661 aattccgctg acttctggtg aagaaactgt gattgcggat attactggaa cgggaataat
      721 tcgtcatatg tggatgacga ttcgtgatcg aacagaattt ggcagttttg ttttacgtga
      781 tttaatctta agaatttatt gggatgggtc ggatagcccg gctgttgaaa gtcctttagg
      841 ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg aactcgttgc ctatagtggt
      901 taatcctac
//
LOCUS       DACDCJ010000221          888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDCJ010000221 DACDCJ010000000
VERSION     DACDCJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.231"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>888)
                     /locus_tag="IV038_003023"
     CDS             complement(<1..>888)
                     /locus_tag="IV038_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8343148.1"
                     /translation="FAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANI
                     RSFFYYFYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAIN
                     IERSSFRKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVID
                     GNAIYENSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSY
                     LFYGNPDLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDY
                     YYVLDYYDLFLTNVKPIKI"
BASE COUNT          323 a          146 c          111 g          308 t
ORIGIN      
        1 aaaatcttaa tcggtttaac atttgttaaa aataaatcat aataatctaa cacataataa
       61 taatccaaaa ataattgtgc ccagttttct aatttctttg atacatccaa atccgcattt
      121 gcttgtaatt cttttttgag tttttccatt atgtgataag ttcgaggttc ttcttctttc
      181 atttcttcga tatacgaacg attaaaaaaa agatctggat taccataaaa tagataactt
      241 ctttcatgaa atgcaataaa attgaatatg agttctggat cctttatatc taaagaaaaa
      301 cggaacatgc tttgtaaaac tttctccaaa ttctcaactg ctcgataaga acaagtattt
      361 tgtcgctgat gtgcatatgc atgactattt tcatatattg catttccatc aattactcca
      421 aaagaataaa ttattcgata aaggaaacac agttcatcgc tgttaaaaga aagattgatt
      481 tgttttgacc acttcttcaa aagattaaaa tgcggatcat ctgagataac tgatttatat
      541 tcattatcta ctcgtacttt tctaaatgaa cttctttcta tattaatggc taaccaaaaa
      601 gaaaatacac gtttttgaaa aatagttaac gtaatatcat aaatcccctc aaaagcaact
      661 atatacttct ctatttcctt ttgttcgata tgaaatggcc acttatcact actttcacgt
      721 gtagaattcc aatagaaata ataaaaaaag ctacgaatgt ttgcctctga tgcattcaat
      781 tctaactgaa atggatgaaa tgttatatga taacgcctaa aatatggaat taagagcttc
      841 gcatatctga aaacagtaga tacactagta aattgctctt tagcaaaa
//
LOCUS       DACDCJ010000222          888 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDCJ010000222 DACDCJ010000000
VERSION     DACDCJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.232"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            64..735
                     /locus_tag="IV038_003024"
     CDS             64..735
                     /locus_tag="IV038_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP8343149.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          345 a          130 c          135 g          278 t
ORIGIN      
        1 gatattatat ggcatgaaca gaattaaaat atcagttaaa ataaaagtta aggagcattg
       61 tgtatgacaa ttaaaactag tctccaaaat caagcttacc aatctattcg ccgacaaatt
      121 atctatgctg acttagaacc tggaaaaaaa gtatcagaaa aaaaattaga agaaacactt
      181 tctattggtc gtacaccaat acgtgaagca ctgattcaac tccgacaaca aggtcttgtt
      241 gatacaattc ctcaaagcgg aaattatatc tcattaattg atttagattc ggccaaaaat
      301 gctcgatttg tcagagaaca tttggaacga caaattatgt tagagtgttg tgccaaaatg
      361 aataaccaaa aaaaacggat tttagaaact ataatttcag aacaagaaaa agctgttagt
      421 caaaaaaatg ctcgtagttt ttttcataat gataatttat tccataaagc ttgttttgaa
      481 attgcagagc gagaagaagt atggcaatgg ttagaaaata acaatacaca cttagaacga
      541 tttcgatggc tacgagtaaa aacggaagga ttaaagtggg aaaaaattat ggaacaacat
      601 tatcaactat ttgaggcatt aacaaaacaa gatccagaag aagctaactt tctaacatcg
      661 ttgcatcttc atttaatgct taatgagcaa aatattgttt tagctactca tccaaaatat
      721 ttcaaaaata tataactttt aactcttttt aaattaaatg gttgttctga tcagaaaaat
      781 atcgcttgtt ttcttattat gtaatatatt tcataaacta ttctgcataa caactattgt
      841 ttgtactttt tacattggat aactaattat gactgctgat aacttttg
//
LOCUS       DACDCJ010000223          874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDCJ010000223 DACDCJ010000000
VERSION     DACDCJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.233"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            82..>874
                     /locus_tag="IV038_003025"
     CDS             82..>874
                     /locus_tag="IV038_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP8343150.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFT"
BASE COUNT          323 a          162 c          162 g          227 t
ORIGIN      
        1 gtaaaagtaa aaaggccata taacagtcct tttacggtac aatgttttta acgacaaaaa
       61 catacccagg aggactttta catgacccaa gtacatttta cactgaaaag cgaagagatt
      121 caaagcatta ttgaatattc tgtaaaggat gacgtttcta aaaatatttt aacaacggta
      181 tttaatcaac taatggaaaa tcaacgaaca gaatatattc aagcaaaaga atatgaacga
      241 acagaaaacc gacaaagtca acgaaatggc tattatgagc gcagctttac gacacgtgta
      301 ggcacgctag aattaaaagt acccagaaca cgtgatggcc atttttcacc cacagtgttt
      361 gaacgttatc aacgaaacga aaaagccctc atggcttcaa tgttggaaat gtatgtatca
      421 ggcgtttcaa ctcgtaaagt atcaaaaatt gtggaagaac tttgtggtaa atccgtctct
      481 aagtccttcg tttctagctt aacagaacag ctagaaccta tggttaacga gtggcagaat
      541 cgtttattat cagaaaaaaa ttatccttac ttaatgaccg atgtactcta tataaaagta
      601 cgagaagaaa atcgagtact ctcaaaaagc tgtcatatag cgattggaat aaccaaagat
      661 ggcgaccgtg aaattatcgg cttcatgatt caaagtggcg aaagcgaaga gacctggaca
      721 acattttttg aatacctaaa agaacgcggt ttacaaggta cggaactcgt tatttctgat
      781 gcgcacaaag gattagtctc tgccattaga aaatccttca ccaacgtaag ttggcaaaga
      841 tgccaagttc acttcctaag aaatatcttt acca
//
LOCUS       DACDCJ010000224          863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDCJ010000224 DACDCJ010000000
VERSION     DACDCJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.234"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          289 a          143 c          105 g          326 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat att
//
LOCUS       DACDCJ010000225          856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDCJ010000225 DACDCJ010000000
VERSION     DACDCJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.235"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(23..298)
                     /locus_tag="IV038_003026"
     CDS             complement(23..298)
                     /locus_tag="IV038_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8343151.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            530..>856
                     /locus_tag="IV038_003027"
     CDS             530..>856
                     /locus_tag="IV038_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP8343152.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNL"
BASE COUNT          302 a          140 c          129 g          285 t
ORIGIN      
        1 tgttttagtc ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag
       61 attttttaaa tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat
      121 attttctaat cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat
      181 actctagagt gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct
      241 ctttcacttg ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat
      301 ttccccctcc tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg
      361 ctcaaaataa aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc
      421 atcgacatac acataatacc tcgataaaaa aggggtgtca acatctttgt aaaatttatt
      481 tacaacaccc cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg
      541 tccaccaata aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag
      601 aaagagtaaa aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat
      661 tgacaaaaaa actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt
      721 atcccgaatt gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga
      781 agaatatatt ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt
      841 tccagataaa aacctg
//
LOCUS       DACDCJ010000226          849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDCJ010000226 DACDCJ010000000
VERSION     DACDCJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.236"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..369
                     /locus_tag="IV038_003028"
     CDS             <1..369
                     /locus_tag="IV038_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP8343153.1"
                     /translation="SVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFA
                     RKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFL
                     YPLSKDKVLENCELLKQKHK"
     gene            379..>849
                     /locus_tag="IV038_003029"
     CDS             379..>849
                     /locus_tag="IV038_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP8343154.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAV"
BASE COUNT          300 a          103 c          167 g          279 t
ORIGIN      
        1 tcggttggct atatgggttt gggtgtgttt aatactgtta tttgggcaaa tattactgat
       61 gttattgatg atcaagaagt aaagtctgat caaagagaag atgggaccgt atatgcggta
      121 tattcttttg ctagaaaaat aggtcaagct ctagctggag gcgtcggagg ttggactctt
      181 tctattatag gctatgactc tttagcaaat gtacaaacgg gagatgtttt agaaaagtta
      241 tataacgctt caacattaat tccatcaatt tgttttctga ttgtaggatt aattttattt
      301 ttcctttatc cattatctaa agataaagtc ttagaaaatt gcgagctttt aaaacaaaaa
      361 cataaatagg agagaaatat gaataattgg atattttgta ttgattcaga tggctgtgtt
      421 atggatacga tgacttataa acacaatctc tttttcggac caattgctgc agagattttt
      481 gatgttcatc atagagaaaa attcctacag gattggaata atattaactt atattcagag
      541 actcgtggaa taaacagatt tttaggttta gttaaaacat tagaaaaaaa tgatgtaaca
      601 tcgattaatc gattaaagca ctgggtatct ctaacaaaag aattgtctaa tccatctttg
      661 aagagggaaa tagaatattc tcagtcagag gatttattaa aagctttaaa atggtcggaa
      721 gaagtcaatc aacagattag tcaagcaaaa ggattagata aacccttttt aagtgctaga
      781 gatactataa aagctctgaa aaaatatggg aagataatta ttgtaagctc agctaataag
      841 gaagctgtt
//
LOCUS       DACDCJ010000227          817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDCJ010000227 DACDCJ010000000
VERSION     DACDCJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.237"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(90..380)
                     /locus_tag="IV038_003030"
     CDS             complement(90..380)
                     /locus_tag="IV038_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343155.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(498..758)
                     /locus_tag="IV038_003031"
     CDS             complement(498..758)
                     /locus_tag="IV038_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343156.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
BASE COUNT          341 a          134 c          115 g          227 t
ORIGIN      
        1 gatcttttta tacttcaaaa aagataacac ccaactttca aaaatatgtt ttggaagttg
       61 ggacaaacga gttaatacat tttaatacat tattttttat tcaaaaaaaa tagcacaatg
      121 gcaaagatcg taagagagat cataagcccc aatgaatgac taaaaagaaa agcagaatca
      181 catgccatat catcccatat ttcttcaaga aaataaataa gcgggaaaaa gggaaggaat
      241 agtagaacat cgatccagca ccattttttt cttcttttga aataacataa aacaataaac
      301 atgaacgtgc agggaagtaa aaacaaagct ttactcataa ctaacaaaat accagctcct
      361 tttatttttt tctcccccac attcaaagag taacaatggt atgtacttaa taatagaaaa
      421 atggatcaac ttgtatgaat aacagctgaa aagatctgca ccaaataaaa aacttccagc
      481 caaaactgac tggaagttta tttttttctg aaatattcta acacaaatat gaaaaagatg
      541 gccgaagcta ataagccgat ttcgtagcaa aacaatgaga taccagagtc gtaataataa
      601 aatcctctaa tcttatggat aaaatagatc atcggacaag cagataagag taagtatttg
      661 ttaggccttt tgaataagta aaagaaagca ctggtcatta tgataaatgg tatccaaaac
      721 aaaagtttac ttaagacaat caaatgatca actcccatat tttttaccaa gagagatcat
      781 aacatcttta tcgtaaaaac aaaatatttt tttgctc
//
LOCUS       DACDCJ010000228          792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDCJ010000228 DACDCJ010000000
VERSION     DACDCJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.238"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..717
                     /locus_tag="IV038_003032"
     CDS             1..717
                     /locus_tag="IV038_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP8343157.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          200 t
ORIGIN      
        1 ttgaaaaaaa ggattttagt aacaattggt ctagcttttt tgttagctgg ttgtaccaac
       61 gggaatgaat cgaaaaaaga atcaacaaaa gaaacagcga aagcaacagt tgcaactact
      121 acaacaacta gcacagagag caagtcaact gcagcaagta cagaaacagc agatacctct
      181 acttcgtcag agtcttccaa aacgactgaa acaactcaaa catctacttc aagttcttct
      241 gtagaggctt catcttcctc ttcagaaagc agcgaacaag ttgaacaaac actgtggaat
      301 gcagacaaat ccgcacaatt agaggcattt atgaccaaat ggggccaaac aatgggacaa
      361 caatacaaaa gttatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa
      421 gctattttga acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg
      481 tcagaaagtg ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag
      541 acagaaccat atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa
      601 gtattggtga cacaacaaaa tcaggggaat cccgataatt atctttactt caacgagaca
      661 gcgaataatg aattgaaaaa tggctttaat caaatcgttt atggacaagc aaattaatga
      721 gataaacaag gggttgtgac atgtctttta tcacaacccc ttgctcagtt tatttgttag
      781 tcttttattt tt
//
LOCUS       DACDCJ010000229          769 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDCJ010000229 DACDCJ010000000
VERSION     DACDCJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.239"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..501)
                     /locus_tag="IV038_003033"
     CDS             complement(<1..501)
                     /locus_tag="IV038_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP8343158.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTGNPKNIRLFL
                     YNLFNST"
BASE COUNT          240 a          129 c          104 g          296 t
ORIGIN      
        1 tgttgagtta aaaagattat ataaaaaaag ccgaatgttt ttaggattcc ctgtcaactt
       61 tctcttttca aaaaaaattt gatatttttt aagatttctt tttagtcttt gtaatacttt
      121 ataagccaca gaataactca cgtgatgctg tttagcaaaa ttttctactg agatatattt
      181 ttcctgaaag aaagccaata ccattttcat agataaagaa tccgttatat aacgagataa
      241 cagagctttg ctagaacatt ttccattaat ttccagacat atatccaatt cactgacggt
      301 tactcgcaaa tttttctcta gatcgaactt ttgtaaatca tgtttcagct tatcgatcgt
      361 ttttttcaag agaaatgtcg atagcgacaa ctcttcttga acattcgata ttgactctga
      421 taaatttgag cttgcttcta aatatttaag taatgaataa cttctgtgat cttctttgct
      481 taaaaaaaac tcataattca ttagtcatca gctctttcta cgtatattac tttccatgca
      541 ttcaccaact agctggggat aaaactgatt tcctggatcc gagtcactgt tgtcagcgat
      601 tcttgtttgt ataatataca tcgtcctgtc ttttttttaa cattttggag agcgttttat
      661 ttggcctttc tcgcgatcaa acctgttata agaattatat catttactag tatgcatttt
      721 tattaataaa atggtatatt ttatattatt ataaaaagag cccaaccat
//
LOCUS       DACDCJ010000230          740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDCJ010000230 DACDCJ010000000
VERSION     DACDCJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.240"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(214..738)
                     /locus_tag="IV038_003034"
     CDS             complement(214..738)
                     /locus_tag="IV038_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343159.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          249 a          100 c           83 g          308 t
ORIGIN      
        1 ttgttaattg tttatttttc tattattaga tatgaaaatc gcatttagat aaaatagaaa
       61 aatatgaata tattttaaat attaagatga gagtcattaa cttatgatag tagtcaattt
      121 gcttttcact aattacaaaa tatgtgatat aggaagttta gttattgttc tcataatgcc
      181 atcctgtttt tagattatct agtaattttt ttgttattta aaattaggag cagtgccaat
      241 tgttttaatt ttaaaggctt tttttatatc ttctaaatta gaagatattt gtatatcaat
      301 atggaatata ttaccttttt tgtatttttt tatgttatct ttagctaaaa catcaaatac
      361 atgtccttga aattgtatcg aggcagctgg atttagttta aattgaggaa actttaccac
      421 agctgattca aaacacttgt cacatttttt tattttttta cctttgtaaa aatattcgat
      481 tgaatattct atttggcacg actcacttgg cagtatatgt atgtcaatat ttttcatctg
      541 tttcataggt atttcaacat ctaaactatt aaaaacatat aattccctgt aattatttac
      601 atctaactct actaaattta tctttggaac aatagatcct ccacccattc caccataata
      661 aaaaaaatct tgttctctat caaaatagca catattaagc tcagtttgtt tatcatcctc
      721 aataatttta taaatcaaac
//
LOCUS       DACDCJ010000231          723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDCJ010000231 DACDCJ010000000
VERSION     DACDCJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.241"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>723
                     /locus_tag="IV038_003035"
     CDS             <1..>723
                     /locus_tag="IV038_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP8343160.1"
                     /translation="FKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNR
                     TVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFD
                     VKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFT
                     ESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTN
                     LFLFDEPTSGLDYINMERIADL"
BASE COUNT          283 a           84 c          129 g          227 t
ORIGIN      
        1 acttcaaagc gactaagaaa cttgctgact tactttgtga tttaaaaaat aaaggattta
       61 ctattgttat agctgaacat cgtttttact atttgaaaga attaattgat caagttctat
      121 ttgttaaaaa tagaactgta aaaactacaa ctattcagtt gttaaaaact gaaaacaata
      181 acgcattaag gaaatttgac atttttaata gttcgattga agttccctat atcgaaaagg
      241 taagtgcaga tgtattaaaa gttgaagaaa ttacttatga aaatattcta aaagacataa
      301 attttgatgt caaagttgga gatgttattg gtttggtagg gaaaaacggt gtggggaaaa
      361 caaccttact aagaatactc atgaagatta ttcgccctac caaaggaaag ataatcgaaa
      421 ataaaaaaaa tttgtcatcg ccgtttttag taatgcaaga aatggattac caatttttca
      481 cagaatcagt gaggagcgag ttagccctgg ggaatgaaat agttagtaaa gatcagcaag
      541 aaaaaatttt aaaaaaaatg gaactttata aaatgaaaga tcaattaccg tttgatttat
      601 ccggtggaga aaaacaaaga ttattggttt ctatagcttc attgtcaaaa actaatttat
      661 ttctatttga tgaacctaca agtgggttgg attatattaa tatggaaagg attgctgacc
      721 tta
//
LOCUS       DACDCJ010000232          717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDCJ010000232 DACDCJ010000000
VERSION     DACDCJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.242"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(74..649)
                     /locus_tag="IV038_003036"
     CDS             complement(74..649)
                     /locus_tag="IV038_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP8343161.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          240 a          153 c          126 g          198 t
ORIGIN      
        1 cttaatttga caaataataa aatgaaaaaa cagagccaga ttagcattat tttgctaatc
       61 cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgg ggcaacatag
      121 tgatgactaa tgaaacaacg acgatagaca acaagcgatc aaggtagtct gtacccgcct
      181 gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca
      241 tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga
      301 tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt
      361 tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg
      421 aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg
      481 tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga
      541 gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac
      601 acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc
      661 atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DACDCJ010000233          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDCJ010000233 DACDCJ010000000
VERSION     DACDCJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.243"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..>704)
                     /locus_tag="IV038_003037"
     CDS             complement(<1..>704)
                     /locus_tag="IV038_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP8343162.1"
                     /translation="SVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVL
                     FLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKV
                     EVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFY
                     GNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSL
                     LKTFFRSKVNYAFLGI"
BASE COUNT          265 a          120 c           85 g          234 t
ORIGIN      
        1 aataccaaga aacgcataat tcactttact tctaaaaaat gttttgagta aactaaagat
       61 atttaacaca tagaaagcat agatacatat aaaagcaatt ataccacttg atacaagtac
      121 ttgaagaaat ccattatgca tgtttccatg tgctcttttt agttcattca catctgtagt
      181 atcaagatct acttttttca aaaattttgc atcgctattc ccatagaaat cggtctctcc
      241 ataaccaaat aacaaagttg gtcttataac tttaagcgca gctttccaaa tcgtaaaacg
      301 accagctgaa atttcggagt tatcactaga atgctgtaat attacttctt tttctggaag
      361 tggagtttta tctgattcat ttgactctat aacttctact tttctatcaa tcgatacagg
      421 aattgttgct aaaaaatcct tagcataatt agtaccaaaa gaaaaatata ccaatagaac
      481 taataatatg cttaccatcc atttgatttt tacaggtagt acttctctaa tgctctttct
      541 aaattttgaa ttggcaaaca acaaaagaaa taatactaaa aaagaacaaa ttactacctg
      601 ggcacctcta gacgaagaaa gtgtaatata aaatatttgt acgataaaat tcatcatata
      661 aaaaacacga atcgtttttg ttatttttga aattaataca ctaa
//
LOCUS       DACDCJ010000234          677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDCJ010000234 DACDCJ010000000
VERSION     DACDCJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.244"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(74..>677)
                     /locus_tag="IV038_003038"
     CDS             complement(74..>677)
                     /locus_tag="IV038_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP8343163.1"
                     /translation="SFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKISQ
                     KFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTETAT
                     QEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQLDA
                     SYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT          174 a          125 c          138 g          240 t
ORIGIN      
        1 ttatttttca cacaaaaaaa ccgagaactt gaataaacaa gtttctcggt ttcatcattt
       61 tccttttatc ggcttagtac cagccgtttg ctaaccagaa tgttttagca gcatcccatg
      121 aaccataacg agatgttaca tattgttctg ctacacgttc ttgattggca gctgaatagt
      181 cgccgtttaa gtatgaagca tctaattgat aacggccgat gtaacgtccg ttagtagctg
      241 tgtatgaacc gcctgattct ttttgtgcga tccattcttt tgcagagctt gaagttggcg
      301 ctgttgcagc agctggggca gcaggagcta cttcaactgt ttcaacaggt gcaacgtatg
      361 tatgttcttg cgttgctgtt tcagttgtag atgctgtatt ttctgttgtt ttgttttctg
      421 ttgttgctga agaatcattt gaagttggga ttgttaattg ttcaccaaca taaatgcggt
      481 tgatgtttgc gatcgaattt ttttcagcaa ttgcatcgat cattgagttg tctcctgcaa
      541 atttttgtga gatcttagat aatgaatcac ctgattttac tgtataaact tcatcagcgt
      601 gagcatttgt agttcctaca aacattgcag tagcaccagc agcaaaagtt gtacctaaaa
      661 caattttctt aaatgaa
//
LOCUS       DACDCJ010000235          673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDCJ010000235 DACDCJ010000000
VERSION     DACDCJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.245"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            447..>673
                     /locus_tag="IV038_003039"
     CDS             447..>673
                     /locus_tag="IV038_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP8343164.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAH"
BASE COUNT          274 a           95 c          100 g          204 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat cgcgaattgt
      361 aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga
      421 aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga
      481 caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg
      541 gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaaggt aagactgctc
      601 ttgattattg gcaccagtat aaagaaaata agaaaaaatg tgggagaaaa gtcattcaat
      661 tacctgctca tga
//
LOCUS       DACDCJ010000236          662 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDCJ010000236 DACDCJ010000000
VERSION     DACDCJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.246"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          207 a          100 c           99 g          256 t
ORIGIN      
        1 ttattaatta tacgggatgt aatcaattag agaataaact aatcataaaa ggtactttta
       61 cattcataat tgtcgctttg agattctact ttggttcact atctgaccca attgcacatt
      121 ttcgatcatt acaagtattg gtgtcgttag aacaactaaa taaaaactag cggtagattt
      181 ttcagaatct atgctaattt ctgtatggat gttccagtga tggcaaattt ctcttttcat
      241 tgtttttctt gtcgcatgat atgccttttt gtgttattca gttaaactat agaaattctt
      301 tcctagacag atcttgatcc caaattttac gtttttgagc atcgatatag tttaaaatga
      361 cattactcat atctaaagag tttagatttt ttaggactaa aactgtcagt tgttcttttt
      421 cttctttctg ttaaagtatt gtcgcgactt ttcgtatacg ttaagatatg tcgtgaaaaa
      481 acaagttttg tgacacaaaa aaacgaatga cttgaggaaa tcacctcaat cattcgttta
      541 aaatgctata tattctttag tttaagatac ggaaattaat gccatcgacc acactagtag
      601 aatgaacgac atttacttgg ccttttttta atttcgttat gtttagcata taaaaatatt
      661 at
//
LOCUS       DACDCJ010000237          656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDCJ010000237 DACDCJ010000000
VERSION     DACDCJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.247"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          235 a          114 c           79 g          228 t
ORIGIN      
        1 taattcgctt gtggcgaaac ccgacctgga gtttgtctct gtattttgaa ccctaaaaat
       61 taatcaaaca gactgatatt ctcataatca gcgcgaaata agccattaag tacagttttc
      121 ctacctattt ttgataaccc gctggttagt taatattcct tttccgagag agataaatga
      181 ttggttaaat ctatatacat ctcaaccttt aaaaatgttt tcgaaatact tcatcagtac
      241 atgtcataca aattaccagt gcattctcaa attctagttc tttaattgat ttcagttgag
      301 gagcagttac ttttggcgga aatttccatt tttgtccaaa cctcttcccc cgatgaaatt
      361 tctttgttgc aattattgca atgaaaaatt tttgtttcaa aaatatacat aattacttcc
      421 tccaataaaa actatctacc tctcaaatta tttttctcta ataagaaaaa cagaaaaggt
      481 aataagatac aacagtgatg attcctaata aagaaaagac tacaataata tttttctaac
      541 ttatcgcctt caaagaaata gtttcttatt tttcactaaa ataaaaaatt tattgcaaaa
      601 aaaatagaag tgacaaaatt atcaatcatt tcaaggtaat ataactacct gagttt
//
LOCUS       DACDCJ010000238          653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDCJ010000238 DACDCJ010000000
VERSION     DACDCJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.248"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..466)
                     /locus_tag="IV038_003040"
     CDS             complement(<1..466)
                     /locus_tag="IV038_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343165.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYL"
BASE COUNT          213 a          115 c           66 g          259 t
ORIGIN      
        1 ccagatatgt gtgttctttt ggtttaaatg tttcaaataa atgaatcttt acaaaattac
       61 tttttgaatt ggctaaaact ttattttgat tcttatccaa tacttgatcc ccttttagcc
      121 ccataccggt atattgtaaa ataccattta accacttatc tttataaaca ttacttcctg
      181 tttgatcact aaacagtaca agagtattag tttgatgaga tcttctcatt ccaccctgtt
      241 tggaacattt aaacttctct gaaatttctt tgttagtata tactctacca acttctaact
      301 cacctaatat gtttttagac caattttctc gccatgtaat aatcttatca agattaaaca
      361 tttctccctc ttttggcatg ccatttctaa ttaaattagc taatacacca gtagaaatat
      421 ctaatttttt tagtaattct ttcttagtaa tcaatgttgg attcataaca atgcagctcc
      481 taatgatatc aatataattg cattagataa cttgaaaaat cgaaaaaaac tcattttgtg
      541 tcaagttatc tgtaatatat aaaaatatca ttattatacc tcatctatgt gaatttttac
      601 tattcttacc ttagcatatt ctttataggt atatcagagc taatcaaact aaa
//
LOCUS       DACDCJ010000239          644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDCJ010000239 DACDCJ010000000
VERSION     DACDCJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.249"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>644
                     /locus_tag="IV038_003041"
     CDS             <1..>644
                     /locus_tag="IV038_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP8343166.1"
                     /translation="LDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEF
                     KQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTE
                     EVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPT
                     ESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT          230 a          118 c          112 g          184 t
ORIGIN      
        1 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
       61 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
      121 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
      181 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
      241 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
      301 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
      361 atggacgcta cttacattcc actaaaacgt caaaccgtat ccaaagaagc gatttatatt
      421 gccattggta tacgagaaga cggcactaaa gaagtactga gttatgcgat tgctccaact
      481 gaatcaacat acgtttggaa tgagctgcta caggatatta actccagagg agttcaagaa
      541 gtcttgcttt ttattacgga cggcttaaaa ggcatgaaag atactatcca tcaaatttat
      601 cctaaagcaa aatatcagca ttgttgtatc catgtatctc gtaa
//
LOCUS       DACDCJ010000240          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDCJ010000240 DACDCJ010000000
VERSION     DACDCJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.250"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>630
                     /locus_tag="IV038_003042"
     CDS             <1..>630
                     /locus_tag="IV038_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP8343167.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQM"
BASE COUNT          273 a           89 c          104 g          164 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg
//
LOCUS       DACDCJ010000241          614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDCJ010000241 DACDCJ010000000
VERSION     DACDCJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.251"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(90..>614)
                     /locus_tag="IV038_003043"
     CDS             complement(90..>614)
                     /locus_tag="IV038_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP8343168.1"
                     /translation="GVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNKLVDELTFY
                     FDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKVDWKRYAGC
                     VAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAAKELAKVNI
                     VAGIAAAAASCALE"
BASE COUNT          196 a          136 c           67 g          215 t
ORIGIN      
        1 cgaatatagt tatggtttaa atagatattt tctttaaaca ttgcttgttt aaaagccata
       61 tgctcttgtt ctaaactttt cataaacttt tactctaatg cacagctagc agcagctgct
      121 gcaattcctg ctacaatatt taccttagct aactctttag ctgcagcttt tcctaattgt
      181 ttttctgcaa tttttaataa taaatccgca gctttttcaa aattaaaagc ttttaatgct
      241 tcaataaaat ctccctgcgt acctatagcc caagccactc ctcctccagg aagagaatta
      301 atggctacac atccagcata tcgtttccaa tcaactttct ccattctagg aacaataata
      361 tgttcataaa agactcttgc tgctcttcct tgttcgttat cttgactgat tcgttcacga
      421 atagcaccaa aatcctttat atggtataca ccatgttcgt ctaaaaaacc aactgtatca
      481 aaataaaaag ttaattcatc tactaattta ttctttattt ctaaatcaac tgtattcact
      541 ttttctgtgg catacacact tgttccagta ctaacaaaag tacttaaacc taataagaaa
      601 acacctaaaa cacc
//
LOCUS       DACDCJ010000242          609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDCJ010000242 DACDCJ010000000
VERSION     DACDCJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.252"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          222 a           78 c          111 g          198 t
ORIGIN      
        1 taattatgtg attgaatgcc gatttttgtt acgcgctatt tttaattatc tgtaagaaga
       61 ataattttat tttactcata ttcgttttca ctttgaagaa gtaaattgca atttcttata
      121 agttaagcag ctatgcaaaa ggctatttat tctattgtga aatataagat gtagttgaaa
      181 caggatatat agaatcaaaa tacgaatctt attttgattt ttctagtttt ttgaaaaaag
      241 caccctttga tcgttgtatt agtggaaaag agaaaaagga gcatacttat catgtcacaa
      301 aactacaact aaatatctaa acaacaccaa tttgatgcct attgaaaaaa agtattgcga
      361 aatgaagcca agtctattag aaaatgaaat aatagaataa aagaaaatgg gaaaccgctg
      421 aatgatgtaa atgaaggtaa gtatccacga actaattttg atgaacaaga tatctatttt
      481 ttatttgtat tggagatact agtttaacag aattaggcag aagtcgccca tcctctatag
      541 gtgggtgatg aatgccgttc ggtatgaggg ttaccgttgg taactcgaag accgacaact
      601 aaggcgtat
//
LOCUS       DACDCJ010000243          598 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDCJ010000243 DACDCJ010000000
VERSION     DACDCJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.253"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(53..>598)
                     /locus_tag="IV038_003044"
     CDS             complement(53..>598)
                     /locus_tag="IV038_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP8343169.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          169 a          110 c          106 g          213 t
ORIGIN      
        1 ttttttggag tggagaaatc cactcctttt ttcatgctct aaaatgatat ttttacacaa
       61 ctgttacggt tgttttacga aattggacgg gactttgcca gtttaatttc tgttttatcc
      121 gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc tcgaaacagt
      181 tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc tccataagtg
      241 aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac tccttcaatt
      301 tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac gtacgacgat
      361 agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc gctaatgcat
      421 tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc ataaaaggat
      481 ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg tatttaaatt
      541 cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga cgatggat
//
LOCUS       DACDCJ010000244          571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDCJ010000244 DACDCJ010000000
VERSION     DACDCJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.255"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..178
                     /locus_tag="IV038_003045"
     CDS             <1..178
                     /locus_tag="IV038_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP8343170.1"
                     /translation="LLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWND
                     LYHANEVLKIAEEK"
     gene            179..>571
                     /locus_tag="IV038_003046"
     CDS             179..>571
                     /locus_tag="IV038_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP8343171.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHL"
BASE COUNT          222 a           65 c          107 g          177 t
ORIGIN      
        1 cttattaaat aataaaataa gttttgaaga aaacattgtt aaagaaatgc ctgaatctgc
       61 taatgatatg tatgagaagg agttgaaata tttctttgaa aacgtagtaa caggcaaaga
      121 aaactggaat gatttatatc atgcaaacga agtattgaaa atagctgagg agaaataaat
      181 ggaaatttta tttactattt gtggtagagc tgggtctaag gggtttaaaa ataaaaattt
      241 gaaatatttg gcaggaaagc ccctagtcca tttcgcttta gcaatgatag atatctatca
      301 acaaaaacat cctgaacata attgcacgac ttgtttgaat acggacagta atgagttagt
      361 agaattgacg actaagttaa atccgtctgt gaaaattgta gaacgtaaac ctgaattagc
      421 tacagatact gtagggaaaa ttgaagtgat acgtgattca ttcttacaac tagaaagaga
      481 agataatata tttgatttaa ttatagattt agatttgact tcacctttac gtgaagtttc
      541 taatcttgaa gaattaatag atgaacatct a
//
LOCUS       DACDCJ010000245          567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDCJ010000245 DACDCJ010000000
VERSION     DACDCJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.256"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(87..464)
                     /locus_tag="IV038_003047"
     CDS             complement(87..464)
                     /locus_tag="IV038_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343172.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          172 a           98 c          100 g          197 t
ORIGIN      
        1 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
       61 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
      121 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
      181 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
      241 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
      301 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
      361 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
      421 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
      481 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
      541 cgcttgttga tggtataaga ttttcgt
//
LOCUS       DACDCJ010000246          566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDCJ010000246 DACDCJ010000000
VERSION     DACDCJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.257"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          201 a           74 c           61 g          230 t
ORIGIN      
        1 taaaatattt ttattaatat tttataactt tacgcaagat cataattttg tcgtgtagat
       61 atttcattat atcactaaag ataataagat ttaatgacct cattaatatt gtttgctagg
      121 tccaaactct tattaaactt tctttgctat aaaacccaaa catagtaata aaagaataac
      181 ttattacctt taaattatac taccaacaaa tggtagtata ttattaattt atataatatg
      241 aaacctaaaa tatagtagta tgttaattta tactaaatta aataaagtac tacctttaat
      301 aggtagtact ttattatcaa gccattattc cttcttctac tagttctttt tgtcacataa
      361 ttttgcaatc aataaatgtt gcgcaataag tataagaatt ccattttcta aatttgaaag
      421 gtgatccata atttcatgtt caagtagttt attaattgtc cacttgtcgc ttcatctaaa
      481 aattttatag gttttaggaa tgctattcgc ttattttagg aagattttaa ctgtttgctg
      541 tagatatata tcatactagt atcgta
//
LOCUS       DACDCJ010000247          563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDCJ010000247 DACDCJ010000000
VERSION     DACDCJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.258"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..128
                     /locus_tag="IV038_003048"
     CDS             <1..128
                     /locus_tag="IV038_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP8343173.1"
                     /translation="NNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKN"
BASE COUNT          202 a           87 c           88 g          186 t
ORIGIN      
        1 tgaacaattt aatagaacaa gaccatcgac ctattaaacg acgaaataaa ttttatcaaa
       61 gtctccgtac agcctcttcc acgattaagg gcatggagac cattcgagga atatataaaa
      121 agaactgaag aaatggaacg ctcttcggct tttcggtgtc tactgaaatc aaggtattaa
      181 tgggaataac agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc
      241 gcaacagaac ccattagcaa tatataaaag aaggtatcaa gagtcacagt gaatttattc
      301 cgaaaagtga aaagagcaaa tataaaattt aataaaaata agaactaaat aggaaaacgt
      361 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
      421 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
      481 tctatttaac tcattagaca ttctatgctc atttttctcc aaatttgttt caactctttc
      541 gtacttaatt ttgcgtattt gaa
//
LOCUS       DACDCJ010000248          556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDCJ010000248 DACDCJ010000000
VERSION     DACDCJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.259"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..539
                     /locus_tag="IV038_003049"
     CDS             <1..539
                     /locus_tag="IV038_003049"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP8343174.1"
                     /translation="ISMLKNKDNVVVYILGHGTKKELLENLIKAYQLQNVIFLLGGQS
                     NPYKYMSRADLYVMPSITEGFPNALVEAMSTETAVLCADCKSGPREILSNRTLDTICE
                     NIEYAESGILVCPMTDSRNYSPQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAEL
                     YSYDAFEHNLLQIMKTFN"
BASE COUNT          222 a           73 c           99 g          162 t
ORIGIN      
        1 ctatatctat gttaaagaac aaagataatg ttgttgtgta catcttagga catgggacaa
       61 aaaaagaatt attagaaaat ttaataaaag catatcaact acaaaacgta atatttttgc
      121 taggtggcca atcaaatcct tataaataca tgtcacgtgc agatttatat gtcatgccat
      181 ctataactga aggttttcct aatgcattag ttgaagcaat gtctacagaa actgctgtct
      241 tatgtgcaga ttgcaaatct gggccaagag aaatattatc gaatagaacg ctagacacga
      301 tttgtgaaaa tatagaatat gctgaatcag gcatattagt atgtccaatg acagacagta
      361 gaaattattc gccccaatat gaagaatgtg ataaaaattt agcgaatgct atagaagaat
      421 tgattgaaaa cgataatttg aggaataagt atgcaaaagc agcaaaaaag agagcagaac
      481 tatatagtta tgatgccttt gaacataact tgttgcaaat tatgaagaca tttaattaat
      541 tcatatgatg aaataa
//
LOCUS       DACDCJ010000249          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDCJ010000249 DACDCJ010000000
VERSION     DACDCJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.260"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IV038_003050"
     tRNA            complement(5..90)
                     /locus_tag="IV038_003050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IV038_003051"
     tRNA            complement(107..178)
                     /locus_tag="IV038_003051"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IV038_003052"
     tRNA            complement(190..262)
                     /locus_tag="IV038_003052"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IV038_003053"
     tRNA            complement(280..361)
                     /locus_tag="IV038_003053"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IV038_003054"
     tRNA            complement(369..441)
                     /locus_tag="IV038_003054"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IV038_003055"
     tRNA            complement(445..517)
                     /locus_tag="IV038_003055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDCJ010000250          527 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDCJ010000250 DACDCJ010000000
VERSION     DACDCJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.261"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..283
                     /locus_tag="IV038_003056"
     CDS             <1..283
                     /locus_tag="IV038_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP8343175.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          182 a           79 c          102 g          164 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tcccttt
//
LOCUS       DACDCJ010000251          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDCJ010000251 DACDCJ010000000
VERSION     DACDCJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.262"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          169 a           87 c          117 g          144 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctacta
//
LOCUS       DACDCJ010000252          511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDCJ010000252 DACDCJ010000000
VERSION     DACDCJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.263"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>511
                     /locus_tag="IV038_003057"
     CDS             <1..>511
                     /locus_tag="IV038_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343176.1"
                     /translation="VSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKL
                     DDKSDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKG
                     TVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPTLP
                     EGTTVTDVTP"
BASE COUNT          209 a           82 c          112 g          108 t
ORIGIN      
        1 cgtaagctta ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc
       61 gaaatggcca gaagaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag
      121 tgcaacaaaa ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt
      181 gactacagaa ttgaataagg aaccagacgc atctgaggga attaagaaca aagaagattt
      241 accaaaagat actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa
      301 aaaagggact gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt
      361 tacagtgaca gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaaaa
      421 agtagaagtt ggaggcacag tagatctaac agataatgtt actaacttgc caacattacc
      481 agaaggaaca acagtaacag atgtcactcc t
//
LOCUS       DACDCJ010000253          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDCJ010000253 DACDCJ010000000
VERSION     DACDCJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.264"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(<1..366)
                     /locus_tag="IV038_003058"
     CDS             complement(<1..366)
                     /locus_tag="IV038_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP8343177.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMD"
     gene            complement(368..>510)
                     /locus_tag="IV038_003059"
     CDS             complement(368..>510)
                     /locus_tag="IV038_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP8343178.1"
                     /translation="EEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSI
                     LI"
BASE COUNT          168 a           91 c           69 g          182 t
ORIGIN      
        1 atccatatgc tcttttgtaa atccaaagtt ttccatcgaa aatactaact cactcgttgt
       61 atttgttgta aagaattgtc ctctaggatc ttgacttacg tagccaatat tttttgatat
      121 actttcttgt gttaaggatt gtagcgagtt attttcaaat aaaatttccc caaccaattt
      181 tccgtcataa agtcttggaa taagtccatt cattaacttt aatagtgtgg atttcccact
      241 cccagaagaa ccagtaatca atgtaagact accttttttt attgaaaaat taaggtcaac
      301 aagcgcttgt tccttaacat ccttatatga aaaagaaaca ttgttaaatt caaacaattc
      361 tttcacctca aatcaaaata cttaagaaaa aaatagtaac ggaaaacagg atgatatagt
      421 ctaataagct catgtttgtt ttatagtaag aacttctttt cacaggagat tctatcccct
      481 ttgttattgc attagcggtc atttcttcag
//
LOCUS       DACDCJ010000254          500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDCJ010000254 DACDCJ010000000
VERSION     DACDCJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.265"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(165..464)
                     /locus_tag="IV038_003060"
     CDS             complement(165..464)
                     /locus_tag="IV038_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP8343179.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          157 a           93 c           62 g          188 t
ORIGIN      
        1 atactagctg tttttgtgtt aattaaagga taaaatacaa tataaagaaa catctgtttc
       61 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
      121 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      181 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      241 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      301 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      361 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      421 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      481 aaatatattt cttcccacta
//
LOCUS       DACDCJ010000255          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDCJ010000255 DACDCJ010000000
VERSION     DACDCJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.266"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>493
                     /locus_tag="IV038_003061"
     CDS             <1..>493
                     /locus_tag="IV038_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP8343180.1"
                     /translation="FSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNPQAF
                     VTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQYFGL
                     KFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILH
                     VEKG"
BASE COUNT          139 a           91 c          115 g          148 t
ORIGIN      
        1 ccttttctgc tagttctttc aatccaacct atagcgagga aatcaaaggc atcacttggg
       61 gattagaatc cggcgccact tttcctggct ttatcactgt tttaatgagc gagctgcaca
      121 accctcaagc tttcgtaacc cgattagaag aactaatttg tttggcagat ttagacggtt
      181 cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg agaagtcgtg cgagactttg
      241 gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag cctaatgatc agtcaatatt
      301 tcggattgaa attcaaaaaa ggagtattgc aaattgatcc tgttgataat ttaacgtttt
      361 cttggaagaa tttgaagttc ggtcaagcct tcatttcgat tgaatgtacg caatccgagt
      421 tgagtatttc aaatttgtca gaaaagccat tgaaaataag tgatacgaaa aaaattcttc
      481 acgtagaaaa ggg
//
LOCUS       DACDCJ010000256          490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDCJ010000256 DACDCJ010000000
VERSION     DACDCJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.268"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          161 a           96 c           57 g          176 t
ORIGIN      
        1 gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat tacgttatta
       61 aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact tgaagggatt
      121 taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc aaacagtagg
      181 atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt agatgtgccg
      241 ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc aatcgaacga
      301 aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa gacatctcga
      361 ttcatattca agataggaat ctttttatca acatcacttt attcgtattt tcctctacca
      421 taaaagcttc acgctccaca aaattgtttt atacactact attcagccca ttcaacgacc
      481 aatgaatttt
//
LOCUS       DACDCJ010000257          483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDCJ010000257 DACDCJ010000000
VERSION     DACDCJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.269"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
BASE COUNT          179 a           64 c           77 g          163 t
ORIGIN      
        1 ttataaagaa aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg
       61 ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca
      121 ttcaggaatg ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga
      181 agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata
      241 aggctcctta ttccgctatt tgaatactct gaagtttaat ctaagaaaga gtaatatttt
      301 gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt
      361 tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag
      421 aaatgtactt tttttgttgt aatgtgcatg aaattcttta agtaaactag ttaacataag
      481 ttt
//
LOCUS       DACDCJ010000258          478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDCJ010000258 DACDCJ010000000
VERSION     DACDCJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.270"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..270
                     /locus_tag="IV038_003062"
     CDS             <1..270
                     /locus_tag="IV038_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP8343181.1"
                     /translation="ELVKLEMAETGAGVRSSRHWKAAANIEFYYNEIQKGFDQMRELD
                     RQTNWSKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            230..475
                     /locus_tag="IV038_003063"
     CDS             230..475
                     /locus_tag="IV038_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343182.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
BASE COUNT          181 a           58 c          116 g          123 t
ORIGIN      
        1 gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
       61 aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg
      121 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
      181 gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa
      241 actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga
      301 atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga
      361 tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga
      421 aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgatac
//
LOCUS       DACDCJ010000259          451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDCJ010000259 DACDCJ010000000
VERSION     DACDCJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.271"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(36..272)
                     /locus_tag="IV038_003064"
                     /pseudo
     CDS             complement(36..272)
                     /locus_tag="IV038_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          148 a           75 c           77 g          151 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaataagg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaataac gccgtttgta taattaagcg agacaaataa
      301 aaaagccatt tatgttacac tccaattgta atacccgcta aagcatacaa ggagagtgaa
      361 cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc
      421 atcaagaaac tccggttgct atcgttgcga a
//
LOCUS       DACDCJ010000260          443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDCJ010000260 DACDCJ010000000
VERSION     DACDCJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.272"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            complement(145..>443)
                     /locus_tag="IV038_003065"
     CDS             complement(145..>443)
                     /locus_tag="IV038_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP8343183.1"
                     /translation="DVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLE
                     KVPFWGEFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
BASE COUNT          174 a           83 c           36 g          150 t
ORIGIN      
        1 aattatacgg actttatctt gttcttctaa acaatcatct atactcatat aatcactcca
       61 ctaattaatc tccccaaaaa gacacagcat taagagatat accaacacaa cagtgcaaat
      121 aactctacat cttccttcgc aaccttattt caataaacca taaaaattat tttttaatag
      181 aaactgaaaa ttaaaactag aaaaagtagt tttaccgaat gtaagagcat catcaccatc
      241 aatatagaaa tacattcttt tccagttaca tttaattttt aaacaaaatt ctccccaaaa
      301 aggaactttt tctagtgaaa acttggtaat ctctttgtaa ttaattcttg ttaaagaata
      361 attaaacact tcatttttac ttgttatttt ttcattgact ataactagtt catctggtaa
      421 aaaaattaac caacaaacat cga
//
LOCUS       DACDCJ010000261          412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDCJ010000261 DACDCJ010000000
VERSION     DACDCJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.273"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>412
                     /locus_tag="IV038_003066"
     CDS             <1..>412
                     /locus_tag="IV038_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP8343184.1"
                     /translation="EYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDAD
                     GLTLDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGT
                     EHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          155 a           74 c           75 g          108 t
ORIGIN      
        1 aagaatatgg caaactactc tatcaaattt ggaaaaagaa aaataaaaaa tccttttatt
       61 catggaaaat ggatgaaacg tacatcaaaa ttaaaggaaa atggcattat ttgtatcgag
      121 ccatcgatgc agatggttta accttggata tttggttacg taaaaaacgg gacacacaag
      181 cagcctatgc ttttcttaag cggttagtga agcagtttga tgaaccgaag gttgtagtca
      241 cagataaagc cccctctatt acaagtgcct ttaagaaact aaaagaatac ggcttttatc
      301 aagggacaga acatcgtacc attaaatacc tgaataattt gattgaacaa gaccatcgtc
      361 cagtaaagag acgcaataaa ttctatcgaa gtttacgcac tgcctctacc ac
//
LOCUS       DACDCJ010000262          380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDCJ010000262 DACDCJ010000000
VERSION     DACDCJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.274"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            1..313
                     /locus_tag="IV038_003067"
                     /pseudo
     CDS             1..313
                     /locus_tag="IV038_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          146 a           47 c           55 g          132 t
ORIGIN      
        1 agaacaaata agattatttt taaagttttt aaaagttaaa gttcaaaata actacttcac
       61 atgttatagt tatgcacacc ctgttttaaa tagtcttcct tttaattctt tagtgcaagg
      121 attgattaca aatattgttt tattagtctg tacaccaaat gaaatatttg taaaaaagat
      181 aggcagtggt atttcttcag taagattaaa agagaaagaa tatactcaaa agattattca
      241 tattcctaag cctgaaatta agcgttttga gataaaagac tattctgctt tagggctgtt
      301 ctcaattggt tactggtgat aaaaaataag aatactaaaa ctatacgtag aatataagta
      361 atcggcatca atcctaatta
//
LOCUS       DACDCJ010000263          380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDCJ010000263 DACDCJ010000000
VERSION     DACDCJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.denovo.275"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..>380
                     /locus_tag="IV038_003068"
     CDS             <1..>380
                     /locus_tag="IV038_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP8343185.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEANLPNILGDKGYISHK"
BASE COUNT          133 a           71 c           62 g          114 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      361 aggatatatt agccataaaa
//
LOCUS       DACDCJ010000264         9930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.092, whole genome shotgun sequence.
ACCESSION   DACDCJ010000264 DACDCJ010000000
VERSION     DACDCJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.092"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..387
                     /locus_tag="IV038_003069"
     CDS             <1..387
                     /locus_tag="IV038_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP8343186.1"
                     /translation="LRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESM
                     YFQKKLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAEN
                     WAKRFFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(530..1471)
                     /locus_tag="IV038_003070"
     CDS             complement(530..1471)
                     /locus_tag="IV038_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP8343187.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1597..2718
                     /locus_tag="IV038_003071"
     CDS             1597..2718
                     /locus_tag="IV038_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP8343188.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            2976..4478
                     /gene="lsa"
                     /locus_tag="IV038_003072"
     CDS             2976..4478
                     /gene="lsa"
                     /locus_tag="IV038_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8343189.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYRLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4545..5072
                     /locus_tag="IV038_003073"
     CDS             4545..5072
                     /locus_tag="IV038_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP8343190.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            5284..6345
                     /locus_tag="IV038_003074"
     CDS             5284..6345
                     /locus_tag="IV038_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP8343191.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6348..7244
                     /gene="murQ"
                     /locus_tag="IV038_003075"
     CDS             6348..7244
                     /gene="murQ"
                     /locus_tag="IV038_003075"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP8343192.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            7258..9240
                     /locus_tag="IV038_003076"
     CDS             7258..9240
                     /locus_tag="IV038_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP8343193.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGARQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9310..9756)
                     /gene="nrdI"
                     /locus_tag="IV038_003077"
     CDS             complement(9310..9756)
                     /gene="nrdI"
                     /locus_tag="IV038_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP8343194.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
BASE COUNT         3224 a         1774 c         2167 g         2765 t
ORIGIN      
        1 cttcgaggaa atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg
       61 gaatcgaggg ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg
      121 tttgaatcta tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat
      181 cagttttatc attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg
      241 gaatttttgc atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca
      301 gaaaattggg caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac
      361 gatctttcct ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa
      421 gcgcaattct tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa
      481 aaaaatgaga accagctgct gatccagctg attctcatca gagagccttt tattctgcgc
      541 caaggattcc ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa
      601 tatcatattt ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac
      661 gatggttttt gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat
      721 cataagtaga agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg
      781 ctgcgataga agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg
      841 cacctaattc agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta
      901 cacaagcttt tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg
      961 cttcttgaaa gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct
     1021 ctagttcgat ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg
     1081 tccacatcgt gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt
     1141 catgacctaa tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga
     1201 tcgaacgaaa catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt
     1261 gtttgttgct ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt
     1321 tgccattaaa gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc
     1381 gatcgatcaa gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca
     1441 ttgctccggc tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc
     1501 ccttttagta tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa
     1561 aaagtgtctt acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat
     1621 tcatttaaag gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa
     1681 gaccgtaaag gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa
     1741 gcaatctatt acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt
     1801 cgtcagcgaa aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt
     1861 gaatctgcac aagtattagg attagattta cttgataaac caacggatca aaccatccga
     1921 cttgcttcta gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa
     1981 caaatcattg tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa
     2041 tctttgggtg cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa
     2101 cttcatttgg atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac
     2161 gtgaccaacc catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga
     2221 ggaacggata cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata
     2281 aaagatgttt gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt
     2341 ggcggagccc tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa
     2401 atcactggat tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg
     2461 attgatcagc aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa
     2521 tatcgaaaac caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt
     2581 gattttctcc ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg
     2641 gaacacaggc aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc
     2701 aacattctcc cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat
     2761 aggacaaagt gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca
     2821 gactgtacct tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt
     2881 tcgatgtatg ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa
     2941 ataggactgg tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc
     3001 tgacattcgg ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg
     3061 acacacaatg gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga
     3121 atatcctgca aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct
     3181 atttcccgca gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta
     3241 tggattttga gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa
     3301 tcttatggag agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac
     3361 tcttatttgt ggatgacact catttcccgt taatcgatga accaacgaat catttggata
     3421 tctctggcag aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg
     3481 tgagtcatga tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga
     3541 gccaactgga actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg
     3601 atgaatttga aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa
     3661 cagcagctga aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag
     3721 tcggattcat cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg
     3781 cacggacgat gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga
     3841 aagaaaaatt attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt
     3901 ctcatcataa acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat
     3961 tcgagccagt tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg
     4021 gcgcgggaaa gtcatccatt atccattatc gtctggggac attcaacggc aaggttgtag
     4081 gagaaaaatc ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca
     4141 atcgagggac attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga
     4201 ataatctccg aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga
     4261 gtatgggaca acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct
     4321 atatatggga tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac
     4381 tgattttgaa cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata
     4441 aagtatctac tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac
     4501 aaggtatggt taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga
     4561 gacacggaga aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc
     4621 tgactgaaaa aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg
     4681 ttccaatcac gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag
     4741 tagttgatcg tctcaatttg ccgatgaccg ctgatgagcg attggtagaa atggattttg
     4801 gggattatga cggactgcca agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg
     4861 ctgtacgttt cccaaatgga gagtcggtct tagatgtgta cgcacggatc gttcctttat
     4921 tgaaagaatg catggaagac gaagaaaatg tctacttgtt agtctgtcat aatgccttga
     4981 taagagtgat caatgcttat tttcatccaa tgcctaatga agggttcttt acattcatgg
     5041 tagacaatac agaattaatt tcttatgagt aattagactt ttgtgttatt taaataaaat
     5101 gtttcaaggc actgcttttg cttagcagtg ccttttttgt ggctgtgtca attcttttgg
     5161 cattataaat ccaattttta gaaataaaat ttaattattt taacaaaagt attgaaattt
     5221 tgtttcttta gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat
     5281 aatatgtttg gcttttcaat atttatgaat gattctttgt cagaagagaa gaaacagtat
     5341 atccgccaaa tggcatccaa tggctttgtc ggtattttca cttccatgca tataccggaa
     5401 gatgatgttt cggcttatca aaatcgattg atcgaactag gaagcgaagc gaaaaagaat
     5461 aaactagagt tgatggtcga tatttcagga gaggcattga aacgagctgg cttttcatta
     5521 gaagatctaa cacccttgaa agcaattggt gtaacagggt taagaatgga ttatcatatt
     5581 tctaatcagc agatcgccga ctggtcacat caattgaaaa tcagtttgaa tgccagcacg
     5641 atcacgccta atgatatcga tgagttgaaa gaagcagcgg ctgatttctc acagattgaa
     5701 gcttggcata attattatcc acgtcttgaa acagggttgg acaaagaatg gtatcaggaa
     5761 aaaaatcaat ggctcaaaac tcaaggattc cttgtacaag gctttgtacc aggagataca
     5821 gagttgagag gtccactgta tcaaggatta ccaactttgg aagaacatcg agggatccat
     5881 cctctggcag gtgctcttga tctattagca agtaacacgg atattgttta tatcggagat
     5941 gcaggtctgt cagaacacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc
     6001 ttgctccata cggaacctgt agatgaagtt ttttacgaat atatattagg aaaacatacg
     6061 aatcggcaag atgatgcaag agatgtcata cgaagcgcag atgcgcgttt tcgagagatt
     6121 ccgccaatcc cagtaagaaa tacagctaag cgtatgaaag gatctattac tttagataac
     6181 gaaaagtatt tgcgctatat gggtgagatc caattaacta aatatgattt gccagcggat
     6241 gaaaaagtga atgtagtagc caaagtcatc aaagaagaac taccgctgat caaccaaatc
     6301 aaagctggta tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca
     6361 atttagagaa cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgaaatga
     6421 gtatttctca agcagtccaa ctcatgaaca aagaagatca gaaagtggcc gctgctgttg
     6481 aaaaagaact gccaagtatc gagccagtga tcgccaacac gattgaatcc tttaaaaaag
     6541 gtggtcgctt gatttatatg ggagctggaa cgagtggtag acttggtgta ttagatgctg
     6601 ctgaatgtgt accgacattc ggtgtagaac cggaaatggt cataggccta attgctggtg
     6661 gagaagaagc aatgactgta gccgtcgaag gagccgaaga ttcttatgaa ttagggcaag
     6721 aagatctaat caagctgaat ttaacaaaaa atgatatagt tattggtatc gcagctagcg
     6781 gtcgtacacc atatgtgatt ggtggattag attacgcaaa atcgattggt gctgttacag
     6841 gaacgatttc ttgcaataaa caagcagaaa tcagcgagca tgcggatttc ccaatcgaag
     6901 tcgatgcagg accagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa
     6961 aactgatttt gaatatgatt tctactatca gtatgatcgg tatcggtaaa gtattcaata
     7021 acttgatggt cgacgtcaaa ccaacaaatg aaaaactaat tgaacgctca aaacgaatca
     7081 tcatgcaagc gacagaagct gactacgaaa cagctgaaaa gtactttgag gaagcagatc
     7141 aaaatgtcaa actggctatc gtcatgattt tgacaaatag tgataaagag gcagctcaag
     7201 caaaattagc tgaagcaaac ggttttgtaa aaggaacctt atgaggggga aaagaccatg
     7261 gcaaaaaacg aattgacttt ggaagaactg gcaaaacaaa tttatcaagg agcaggcggt
     7321 atgggcaatg ttgaaagcat tgtccactgc atgactcgtg tacgtatgaa ggtcatcaat
     7381 gatgatcttg taaatgtctc ggaattaaaa gcgattccag gagtacttgg cgtagtagag
     7441 gatgaacagc tccaagtcat catcggacct gggaaagtga acaaagtagc gcaggaaatg
     7501 gttgacttag caggcgtgaa attaggagaa aaacttcctt ctgctagtgc aactacagca
     7561 agttcttctg catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca
     7621 caaaaatcga aacaaaaacc ctctaaaatc aaatcggtat taaaagacat ctctaatatc
     7681 tttgtaccaa tgatccctgc atttgtcggc actgggatcg tggcagggat tgccgcagtt
     7741 ttagcaaact tagtcacagc tggaacacta gacgcaggaa catggcaaca atatatcgat
     7801 gtgatgaaca tcttgaaaaa cgggatgttt agctatctag ttatctatac aggtatcaat
     7861 gctgcacaag tttttggtgc aacgccaacg cttggtggag ttatcggggc tgttgtgatg
     7921 ctgacaggga tgaatccaga agcaccactg aaaaacttgt ttaccggtga agctcttgca
     7981 actggacaag gcgggatctt aggtgttatc ttcgcagttt ggttattgtc aatcgttgag
     8041 aaaaaactgc ataaagtagt acctgatgcg gtagatatca tcgttacgcc aactctttcc
     8101 ctgattgcca tcggtctgat cgaaattttc ttcatcatgc ctttagcagg gttcatttct
     8161 gacggcatgg tcggtgggat caactgggta cttggtgttg gaggagcatt tgccggcttc
     8221 gttctaggta cattattctt gccaatggtc atgttcggac tacatcagat cttgacgcca
     8281 atccatgtac aaatgattga tgaaactggt cgtaccttat tgcttccaat ccttgcgatg
     8341 gccggagcaa gacaagtggg tgcagcattg gcattatgga tccgctgcaa aaaagacaaa
     8401 gaattaacag aaatgatcaa aggggcttta ccagtcggaa tcttaggaat cggcgaacca
     8461 ttgatttacg gagtaacatt acctctagga cgtccattta ttactgcttg tatcggtgga
     8521 ggtatcggtg gggcagtgat cggaatgatc ggaaatgtcg gagcaatcgc tattggacct
     8581 tcaggagccg ctttgattcc attgatctct gatggtaaat ggttaggcta tatccttggt
     8641 ttattggctg cttacgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac
     8701 cgtttagaaa aagaagaact agcgaccgaa acagccacag taaatgaagt acctgaaaaa
     8761 gcagtacctc aagcttcagc tgaaagtgtg actatcttat catcagttgc tgacggaaca
     8821 gttgtttctt tagataaagc aagtgaccca gtttttgcac aaaaaatgat gggtgaaggt
     8881 tactttgtag aaccagaaaa cggacatatc tattccccgg ttactggagt aataagttct
     8941 gttttcccaa caaaacatgc tatcggcatt acgacaccaa atggcttaga aatcttgctt
     9001 cacatgggca tcaacacagt tgacttaggc ggtacgccat tcgatgtgaa agtaacagaa
     9061 ggacagcaag tgacacctga tacgcttgta gcagaagctg acttagaagc aatcaaagca
     9121 gcagacaaag aaacctccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt
     9181 gtcttggaaa aaacaggtaa agcgaaagca aaagcacctg tgatggatgt caaagcttaa
     9241 taagaataga aacaatagag aacataaaaa gaagaagccg aaatttattt ttcagcttct
     9301 tcttttgtgt tatttatttt tttctgccaa aatttgataa acgcgtgtca catcagatgg
     9361 tgttccccgt aattcataat ctgccaagaa aggaaagccg aatctttgtg cgtattgttt
     9421 ggctgtcaga caatattgat tgttgaagtt tttattaccg ctaccgacta caccaaggca
     9481 aagtttatga ttgtcttcgt attccagata ttcgcgcatc gtttcggtca agatttctgt
     9541 gtcaccgttg tctaacccat tcccgccgtc taaataagta ggaacgaaag taaaaaatgg
     9601 ttctgtttct ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt
     9661 gacaggatct tctgcatgca ttttttcagc atattctgct agatgtttgg caaaagcacg
     9721 cgtatttcct gaaatcgata tatataaaat gttcatggat catcccccaa ttctttccct
     9781 ttagtatagc atctttttaa gtttttttgg tggtgtaatt tggaaaggga ctaggcgctt
     9841 tgcttgtata atgagaaaaa agtcggtaga attatatttc tattaaagtt tttattaatt
     9901 aagtaaaaat aaaaaaggtt tgttatactt
//
LOCUS       DACDCJ010000265         4830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.139, whole genome shotgun sequence.
ACCESSION   DACDCJ010000265 DACDCJ010000000
VERSION     DACDCJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.139"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..427
                     /locus_tag="IV038_003078"
     CDS             <1..427
                     /locus_tag="IV038_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP8343195.1"
                     /translation="RFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAYESGKMPKEVR
                     DQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPIGGFEADQLAE
                     AFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            781..2259
                     /gene="msr"
                     /locus_tag="IV038_003079"
     CDS             781..2259
                     /gene="msr"
                     /locus_tag="IV038_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP8343196.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2306..2752
                     /locus_tag="IV038_003080"
     CDS             2306..2752
                     /locus_tag="IV038_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP8343197.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2951..3466)
                     /locus_tag="IV038_003081"
     CDS             complement(2951..3466)
                     /locus_tag="IV038_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP8343198.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3583..4784
                     /locus_tag="IV038_003082"
                     /pseudo
     CDS             3583..4784
                     /locus_tag="IV038_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1464 a          806 c         1048 g         1512 t
ORIGIN      
        1 ccgtttcaat acgagacaaa atgatacttc ttctgcaatg gtcatgattt ttggcgatat
       61 gcaatgttac gaatatggcg aagaaatcta taaccaagct tacgaatcag ggaagatgcc
      121 aaaagaagtc agagatcaac aattagctgc gatcattcct tattacaaga gtttttcaag
      181 agaagaaatg aacgatgtag tcaaagtcga ttctagctta gcggctatgc aattcatgtt
      241 agttgcgcgt gatcatggct atgaaacaaa tccaattggt ggattcgaag cagatcagtt
      301 ggcagaggca tttggtttgg ataaagaccg ttatgttcca gttattattt tatcaatagg
      361 aaaagcggta gaagaaggtt acgagtctgt tcgtttagca ccagaaaaga tcactacgtt
      421 cgaataaaag gttttcagtt actattcagt agctattgct ttttttgaat cgtgtgtggt
      481 aacctaataa aaaaggaggt cggaaaaatg actgcatcga tgaaattacg tttcgaactt
      541 ttgaataaca actaaataaa ttcaaaagac atgtataaac aacaatttca tggatggagt
      601 atgtccattt tttcgaacga cagtagaata ggtgaaagct atgacagatt tggtgattta
      661 gtcatagtta tttcgttcag agtaggaaaa gatgtgatgc agtttttttg caaacagatt
      721 tttctgctct tttttagtat cgataattgt tgttttacat gaaataggag tgtaaaacat
      781 atggaaaatt tagcagtaaa tataacaaat ctacaagtca gctttggcaa ccagctagaa
      841 ctatctattg attctcttcg tgtctatcag caagatcgta tagggatcat aggagaaaat
      901 ggtgtcggta aatcgacttt gctcaaacta atagccggtg aactttttcc cgatcatggg
      961 aaaatccaaa cagagatcac cttcaactac ctgcctcaat taacctatct cgctgaagca
     1021 aaggacctaa atttggaatt agccagtcat ttccagttaa gactggaaga aacttcagag
     1081 cggaaatgga gcggagggga agaacgaaag atcgagttga tacgtcttct ttcttcttat
     1141 gaacaaggga tgcttctaga cgagccaaca acccatctag atagaaaaag tattgatcga
     1201 ctgattgaag agcttcgtta ttattatggt acgctggttt ttgttagtca tgatcgctat
     1261 tttctagatg agctagcatc gaaaatctgg gaagtaaaag acggagaaat ccgagagttt
     1321 tcggggaatt atagtgccta tctcactcaa aaggaattgg agaaaaagac tcagctacga
     1381 gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca agaaaagaaa
     1441 aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca aatcagaccg
     1501 gatcggttgt cttcctctaa acaaaaagac agtgtacaaa aagccatcca aaagaatgcg
     1561 aaaacattag agagaagact ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa
     1621 caaatccgtt ttccagtacc aaaatctctt gaactccaca gccgctatcc catcatggga
     1681 caaaatgtcc aattggaaag aagtgggaga acattactgg taaatggtga ttttcagttt
     1741 tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac aaccttatta
     1801 gaacatatcc gcaaacaagg agaaggaatc cttctctctc cgaaagtaag ctttcaagta
     1861 tatcagcaaa agggttatca aatgacatct gaagaatcca tcattcgttt tgtcatgaga
     1921 caaacagagt tttcagaatc gcttgtccgt agtttgctga atcacttagg gtttgctcag
     1981 gaaactttga cgaaaccgtt atgtacatta agtgggggag aagcgacccg tctgacgatt
     2041 gctttgcttt ttactaagcc aagtaatgtg ttgctgttag atgaaccgac taattttatt
     2101 gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa tatatccggg aacgattttg
     2161 tttacttctc atgattctta ctttgtcgag cgtacggctg atgaagttta tgaaataaaa
     2221 gggcagaaaa taaaaaaagt acttacgaga aatttttaaa tgtgctacga tgattttcgt
     2281 gacaaattta aaggagtggt acagattgcc tacaaataaa aaagaaggaa taatttttac
     2341 aacaatgatg tgttttttga tggtcttggg gatgagcgcg tataatttat ggctgcatca
     2401 agatcttcat ttatcaagtt tgttgattgg tctagtgcct ggattcgtgg tggctttcat
     2461 tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt gcctttaaac taccagtaaa
     2521 tccagaaagc aaggtccaaa tgatcttaac tatttcatgc ttgatggttt taggaatggt
     2581 gacatgtatg tctttattcg gtgtgatcat ggaacaagga atacctgatc aattattctc
     2641 tgcttatatg aatgcttgga aaatgaatgt gattgccgct ttaccgttgc agttgatcat
     2701 cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa agtaatgaat gatggaacac
     2761 tgttatttga aaaaaggcct tggcatgctt tttgtcatag tctcttgaat gcagttctag
     2821 aaaaatagtg atccgaacga taggttttgg cctgtcgttc ggatcgcttt ttcttttcca
     2881 actgtattgc tcttttattg aagataattt gctcttcaat agatatttct agaaaggttc
     2941 tgtttatccc ttactcaaaa aactgctgca tttgttctct tacttgctga caccattgga
     3001 gttcctgctg acaaagcata attctgcgct ggatcacaac ataacgtcca aattcttgtt
     3061 tcttgtctag cgagaggtca ttcaattttt tttgattttt ttgcatgatt ttgttcagct
     3121 gttgttctct agtgaatagc agtccagtag ctgttgtttt atcgagcgtc gaaaaagcat
     3181 agatttttgc tagaaattca tctttttgcg taggagcggg agtttcctct ttgatccatt
     3241 cctctacaag agcacgtcct tcatcagtca agtggtagat ctttttttga gagtgctcgt
     3301 ctttgtccaa aacggtcaca taaccctgct ggtctaactt ggcaagcgtt gtatagatct
     3361 gactgtgatg ggcttcccaa aaaattgtga tcagctgctt caattcatac ccagaacgtg
     3421 gtttggataa aagcacgcat aataatatat aggctaaagt attcataaaa atcctacctc
     3481 tcgtcttatt ttctttatta tatcacagta aagtttccac gttttacatg tgcatattta
     3541 catgtaaaat aaatcatgta tgatgctaat agagagaaag ggatggaaat attggagaat
     3601 caaatagata tacaagaaca aacaaatgaa gaaacaaagg ggaaaagttc agcgctagtt
     3661 gtagcagcta cctgtatgat cgcttttatg ggagtgggtc tagtagaccc aatcttaaaa
     3721 acgattgctg cacagttaaa cgctacgcca gctgaaacga ccctgttatt tactagttac
     3781 atgttagtca ctggtgtggt gatgctattt acaggtttct tatctagtcg gatcggtttg
     3841 aaaaaacatt gatgacagga ttgtttatca tcgttgtatt cgctggtctt ggcggattat
     3901 ctgggaatat cggaatgctg attgggttac gtgccggctg gggtgtcggg aatgctttat
     3961 ttgtctctac tgctttagca acgatcgtca gtattttagt tggtaacaca gaaaaagcaa
     4021 tcatgatgta tgaagcagca cttggctgcg gaatggctgt tggccctcta gtcggtgggg
     4081 tcttgggcag tggttcatgg cgttatccat tttttggggt agcatttttg atgttttgcg
     4141 gttttatagc cctagcggta cttttacctg aaactgagaa accaaagaga aaagtaggcc
     4201 tttgggaagg tgtcaaagcg ttaagcaatc ataaacttcg ttcagttgga ctaatcgcat
     4261 tgttatataa tttcggattc tttactttac ttgcgtatgc gccattttta ttgattggct
     4321 attctgaatt agaagtcggt tttgtatttt ttggctgggg tgtgcttctg gctattgctt
     4381 cgattttcgt tgcaccggct cttgagcgga agtttacgac ttatcggaca attttagcag
     4441 catttgtctt attcattatt tgtttgctgt tattaggagc tggaacttct atgccagtcc
     4501 ttgtaccgat cagcattgtg ttagcaggat ttttccaagg aattatcaat acactgttga
     4561 cgacgattgc tatggaaata ccaggtctgc aacgaaacgt tgcttcgtcc agctatagtt
     4621 ttgtccgctt tttcggtgga gctttagcac catttattgc aggtgtgatt ggtgaacata
     4681 ttggtgaaag ctatgcgttt tattttgcag gtgccattgt tcttatcagt gtatgttgta
     4741 tcatttatca ccgtaattat tttgctacaa atgaagaaga ataagaaatc agaggttgtg
     4801 aaaaagctgt cctgcatcaa gtcataagaa
//
LOCUS       DACDCJ010000266         4691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.143, whole genome shotgun sequence.
ACCESSION   DACDCJ010000266 DACDCJ010000000
VERSION     DACDCJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.143"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            245..1213
                     /locus_tag="IV038_003083"
     CDS             245..1213
                     /locus_tag="IV038_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP8343199.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IV038_003084"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IV038_003084"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP8343200.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IV038_003085"
     CDS             2243..2851
                     /locus_tag="IV038_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP8343201.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /gene="vanY"
                     /locus_tag="IV038_003086"
     CDS             3279..4190
                     /gene="vanY"
                     /locus_tag="IV038_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP8343202.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4654
                     /locus_tag="IV038_003087"
                     /pseudo
     CDS             4343..4654
                     /locus_tag="IV038_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1480 a          817 c         1060 g         1334 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacta tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacggttct gttgcaaagt tttaaataaa
     4681 gaataaaatc c
//
LOCUS       DACDCJ010000267         3986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.151, whole genome shotgun sequence.
ACCESSION   DACDCJ010000267 DACDCJ010000000
VERSION     DACDCJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.151"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..712
                     /locus_tag="IV038_003088"
     CDS             <1..712
                     /locus_tag="IV038_003088"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP8343203.1"
                     /translation="IWDEWAFERYIKSPDYNGPDMTDFGHRCLTDPEFNEEYKKQSKL
                     FCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFIDQIKQVIEAIKKTPDSRRLIV
                     SAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRSGDVFLGVPFNIASYALLTHLI
                     AHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDFEMED
                     IQLEGYDPHPAIKAPIAV"
     gene            732..1259
                     /locus_tag="IV038_003089"
     CDS             732..1259
                     /locus_tag="IV038_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP8343204.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1322..1975)
                     /locus_tag="IV038_003090"
     CDS             complement(1322..1975)
                     /locus_tag="IV038_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP8343205.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2107..2949
                     /locus_tag="IV038_003091"
     CDS             2107..2949
                     /locus_tag="IV038_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP8343206.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3107..>3986
                     /locus_tag="IV038_003092"
     CDS             3107..>3986
                     /locus_tag="IV038_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP8343207.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLE"
BASE COUNT         1427 a          721 c          795 g         1043 t
ORIGIN      
        1 tatttgggat gaatgggcat ttgaacgcta catcaaaagt ccagactata atggaccaga
       61 tatgactgat tttggccata gatgtttgac agatcctgaa ttcaatgaag aatataaaaa
      121 gcagagcaag cttttttgcg agaagatcct aaccgatgat tcctttgctg aaacatacgg
      181 cgatcttgga cacatctacg gctatcaatg gcgtcactgg gaaacaaaag acggtggctt
      241 tatcgatcaa atcaaacaag tgatcgaagc aatcaaaaag actcctgatt ctagaagatt
      301 gatcgtttct gcatggaatc cggaagatgt tcctagcatg gctttgcctc cttgtcatac
      361 gatgtttcaa ttttatgttc aagagggacg gctaagctgc caattgtatc aacgcagtgg
      421 ggatgtgttt ctaggcgttc cgttcaacat tgcaagttat gcgttgctga ctcacctaat
      481 tgcacatgaa acaggtttag cggttggcga atttgttcac acattaggag atgctcatct
      541 ttatatcaat catatcgaac aaatgaaaga acaattgagc cgagaaatac gcgcatttcc
      601 gactttagtt ttgaatcagg aaaagcagtc agtatttgat tttgagatgg aagatatcca
      661 attagaagga tatgacccac atccggcgat caaagctcct atcgctgttt agaatcagaa
      721 aggatgagaa aatgttagct gcaatttggg cacaggatga aaacggattg attggaaaag
      781 aagaccgtct tccttggcgt ttgccaaatg atctgaaatt cttcaaacaa atgacagaag
      841 caaatacact agtgatggga cgaaaaacat tcgaagggat gggtagccgt ccgttgccga
      901 atcgtcagac gattgttttg acgagagatt cttcctataa agcagaaggc gtgcatgtca
      961 tgcatagtgt ggaagacgtg cttgctctgg aaaaagaaac agacggtatc tttttcatcg
     1021 ctggagggtc agctgtttat caagaatttc tgccattttg tacgatatta taccgtacag
     1081 tcatccatca tgtattcgat ggagatgctt attttccacc tgttgactgg tctgattggt
     1141 cactgatcaa tactagtcaa ggagaaatgg atgaaaaaaa tcgttatccg catcaatttg
     1201 aaacgtatca gcgtaatgag aatcctgtaa aaagcgggac aggcgaattg acgatataaa
     1261 aaagctatgg atagactagc tgttatttag agctgtccca tccatagctt tatttttttt
     1321 attaagtaaa gaataaaaca gagaagtaca taaaacctgc acctaacata acaaacaaat
     1381 gccaaacaac atgcatatag cggatagaat tcaagctata aaagaatgcg cctatcgtgt
     1441 aagaaagccc gcctgctacc atcaaaataa caccagtcat tccaagagag tgataaagag
     1501 gaacagcagc cacgacacag agccacccca taaaaatata gatgatcgta gatacttttg
     1561 atactctctc ccgtttatgc aacgtcaacg ctttatatat aatgccaaga atcgcaaaca
     1621 accagattaa tacaaataaa gtccaaccta gccatcctcg gatactcagc aaacaaaaag
     1681 gagtataact tcctgcaatc aaaagaaaaa tcgaactatg atcgaatact tggaacacct
     1741 tttttgcttt tgtaaaaatc aatgaatgaa acaaagtaga cgttaaaaat aaaagaatca
     1801 tcatcgatcc atagatagca taagagacaa tatggaggga ggaatgcaag tgggcacctt
     1861 ttactaaaag aaccactagt cccgcgatac tcaatccagt cccaattcca tgggtgactg
     1921 cattaaacac ttcatttaat atttcatacg ttcgattaaa tttcgttttt tccatgaaaa
     1981 cggctccttt actaggtgta attaatgaaa aatcattatt tgtctgttat actaaaaata
     2041 gaaattgtct tacatgtagt ttaacatgaa taaggcatct tgttaaagaa agagagagag
     2101 aaaataatga caaatgtaaa aatcgtaaca gattcttcat gtacgatgga aaaaaatgta
     2161 cgcgatgctt taggcattcg tgtcatgccg ctatccatca tgatcgacgg aacagtatat
     2221 cccgatgatg accaactaga cggcgaaaga tttatggaaa tgatggcgaa agcaaaaaat
     2281 ctgcccaaaa caagccaacc accaattgga gaattcatct cgatgtatga tgagctagga
     2341 gaagacggca gtgaagtgat ttcgatccat atgacaaaag gcttgagtgg aacagtagaa
     2401 gcagcaagac aagccagtaa tctaacaaaa acgaaagtaa cggttatcga cagtgatacc
     2461 actgaccaag gattatcttt tcaggtaatt cgagcagctg aattagcaaa agaaggtaaa
     2521 acagtagaga aaatccttga agaagtcaaa aaagtacaag acaatacaaa attatacatc
     2581 ggaatatcca cactagataa cttggtaaaa ggtggaagaa tcagccgaac aactggtttg
     2641 ctgtctagtg ttttgaacat acgcgtggtg atgaatttcg cccattctga attaatccct
     2701 gtaactaagg ggcgaggaaa gaaaacgttc gataaatggt ttaataattt gaaaaatgaa
     2761 ctgaaagaat tgccaaatgt gcgacaaatc ggtatttcac atgcggatgc aaaagaattg
     2821 gcgcaagaat tcaaagaggg tttgcaagaa ttattcccgg acatgcatat ccctgtactg
     2881 catacgaatc cggttatcgc tacgcatact ggtaaagacg cgtttgcaat catgtattat
     2941 gtagattagt cgaaaagagg ataagccctc agcctgaaag gcttgcagct tattctcttt
     3001 ttctttataa aattaggaaa ataattgttt tcttttttca taaagactac ctttttgata
     3061 aaatttttga taacattaga accaatcgaa tacaagaggt gaaaaaatga aattaacgaa
     3121 acgacatttg atcctcgttt tgttcccatt attaattgcg atcatcatct atgcaggttt
     3181 agctgcttct ataccgaaag ctggcccact aatgaaacca gtaactactg tgagagaaga
     3241 tccaaataaa aaagaaaaac ttcactacat agctatcgga gattcgctga ctgaaggagt
     3301 aggagatcaa accaagcaag gaggattcgt tccccttgtc gcaaatgatc taaaagaccg
     3361 ttataatttg accgcggttg aaacagaaaa ctatggtgta aatggtgaac gaagtgacca
     3421 gatcttaaag cgagtaaaaa caaaagaaga aattcgaaac aatatcaaaa cagctgattt
     3481 tatcacactg actgttggcg gtaatgattt gatgaaagtt atccaaaata atttattcgg
     3541 gttaacggtc aattccttta aaaagccact gaaaaactat caggaaaatg tcacagaact
     3601 attggaagaa tttcgatccc tcaataaaac cgcacctatt tacattttag gcatctacaa
     3661 tccattttat ttgaactttc ctgagatcac tgacatgcag acaattgtta acaattggaa
     3721 caaaggaaca gaaacggtga caaaaaaaat gccgaactgc tactttatcc caatcaatga
     3781 tttgctttat caaggattga atcaggaagt aggtgtcaca gaagaaggaa cagatcaaac
     3841 aactagcacg acagaaaatc taaatggcat caaaaataat gtgctctatg aagaagatca
     3901 tttccatcca aataatttag gttatcaatt gatggcaaat gcagtgagag atcagctgat
     3961 cgagacacaa aaccaatggt tagaga
//
LOCUS       DACDCJ010000268         3530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.160, whole genome shotgun sequence.
ACCESSION   DACDCJ010000268 DACDCJ010000000
VERSION     DACDCJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.160"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            109..192
                     /locus_tag="IV038_003093"
     CDS             109..192
                     /locus_tag="IV038_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP8343208.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IV038_003094"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IV038_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP8343209.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IV038_003095"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IV038_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IV038_003096"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IV038_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IV038_003097"
     CDS             2348..2593
                     /locus_tag="IV038_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP8343210.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..>3530
                     /locus_tag="IV038_003098"
     CDS             2696..>3530
                     /locus_tag="IV038_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP8343211.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FL"
BASE COUNT         1273 a          526 c          649 g         1082 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa
//
LOCUS       DACDCJ010000269         3175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.169, whole genome shotgun sequence.
ACCESSION   DACDCJ010000269 DACDCJ010000000
VERSION     DACDCJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.169"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..790
                     /locus_tag="IV038_003099"
     CDS             56..790
                     /locus_tag="IV038_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8343212.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            823..1731
                     /locus_tag="IV038_003100"
     CDS             823..1731
                     /locus_tag="IV038_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8343213.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1728..2270
                     /gene="sat4"
                     /locus_tag="IV038_003101"
     CDS             1728..2270
                     /gene="sat4"
                     /locus_tag="IV038_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8343214.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2363..3157
                     /locus_tag="IV038_003102"
                     /note="aph(3')-IIIa"
     CDS             2363..3157
                     /locus_tag="IV038_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8343215.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         1081 a          477 c          753 g          864 t
ORIGIN      
        1 gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag ttcagatgaa
       61 agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga gtcgctcgca
      121 gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac ctgattttaa
      181 gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat atgcgatgga
      241 aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg aagtagcaaa
      301 aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag atgtggattt
      361 cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg tagcagacta
      421 tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt tagtttttac
      481 agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata acgaaaaagg
      541 agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga cagctatgtt
      601 tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa atacactgct
      661 ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga acatgatgga
      721 tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa
      781 gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc agaaaaagaa
      841 atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat tgtgaccctt
      901 gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta tgatattaca
      961 tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa tcaatttggg
     1021 aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga agaaaaggga
     1081 ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt attgcccttg
     1141 gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat tgataaagat
     1201 tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag aaagccaagc
     1261 gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta tgttattaaa
     1321 ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat tgttcgccat
     1381 gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa attaagtgta
     1441 ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga gaaacttttg
     1501 tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct atgccatcaa
     1561 ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta tccggagtat
     1621 gataggaata taacaaaata taccagggac atgtataaaa aatacactgg taaaaccggc
     1681 tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg attacagaaa
     1741 tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag gtgataggta
     1801 agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc tatgaagcgc
     1861 catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca gattgccttg
     1921 aatatattga caatactgat aagataatat atctttacta ccaagacgat aaatgcgtcg
     1981 gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat atcgccgtat
     2041 gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg
     2101 caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt
     2161 gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
     2221 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
     2281 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
     2341 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
     2401 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     2461 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     2521 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     2581 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     2641 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     2701 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     2761 gaatagctta gacagccgat tagccgaatt ggattactta ctgaataacg atctggccga
     2821 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
     2881 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     2941 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     3001 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     3061 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     3121 aataaaatat tatattttac tggatgaatt gttttagtac ctagatttag atgtc
//
LOCUS       DACDCJ010000270         3175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.170, whole genome shotgun sequence.
ACCESSION   DACDCJ010000270 DACDCJ010000000
VERSION     DACDCJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.170"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            56..790
                     /locus_tag="IV038_003103"
     CDS             56..790
                     /locus_tag="IV038_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP8343216.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            823..1731
                     /locus_tag="IV038_003104"
     CDS             823..1731
                     /locus_tag="IV038_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP8343217.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1728..2270
                     /gene="sat4"
                     /locus_tag="IV038_003105"
     CDS             1728..2270
                     /gene="sat4"
                     /locus_tag="IV038_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP8343218.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2363..3157
                     /locus_tag="IV038_003106"
                     /note="aph(3')-IIIa"
     CDS             2363..3157
                     /locus_tag="IV038_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP8343219.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT         1080 a          478 c          753 g          864 t
ORIGIN      
        1 gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag ttcagatgaa
       61 agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga gtcgctcgca
      121 gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac ctgattttaa
      181 gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat atgcgatgga
      241 aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg aagtagcaaa
      301 aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag atgtggattt
      361 cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg tagcagacta
      421 tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt tagtttttac
      481 agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata acgaaaaagg
      541 agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga cagctatgtt
      601 tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa atacactgct
      661 ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga acatgatgga
      721 tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa
      781 gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc agaaaaagaa
      841 atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat tgtgaccctt
      901 gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta tgatattaca
      961 tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa tcaatttggg
     1021 aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga agaaaaggga
     1081 ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt attgcccttg
     1141 gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat tgataaagat
     1201 tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag aaagccaagc
     1261 gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta tgttattaaa
     1321 ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat tgttcgccat
     1381 gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa attaagtgta
     1441 ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga gaaacttttg
     1501 tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct atgccatcaa
     1561 ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta tccggagtat
     1621 gataggaata taacaaaata taccagggac atgtataaaa aatacactgg taaaaccggc
     1681 tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg attacagaaa
     1741 tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag gtgataggta
     1801 agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc tatgaagcgc
     1861 catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca gattgccttg
     1921 aatatattga caatactgat aagataatat atctttacta ccaagacgat aaatgcgtcg
     1981 gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat atcgccgtat
     2041 gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg
     2101 caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt
     2161 gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
     2221 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
     2281 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
     2341 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
     2401 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     2461 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     2521 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     2581 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     2641 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     2701 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     2761 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     2821 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
     2881 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     2941 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     3001 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     3061 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     3121 aataaaatat tatattttac tggatgaatt gttttagtac ctagatttag atgtc
//
LOCUS       DACDCJ010000271         2825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.172, whole genome shotgun sequence.
ACCESSION   DACDCJ010000271 DACDCJ010000000
VERSION     DACDCJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.172"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            174..260
                     /locus_tag="IV038_003107"
                     /pseudo
     CDS             174..260
                     /locus_tag="IV038_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
     gene            261..2201
                     /gene="tet(S)"
                     /locus_tag="IV038_003108"
     CDS             261..2201
                     /gene="tet(S)"
                     /locus_tag="IV038_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAP8343220.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2289..2456
                     /locus_tag="IV038_003109"
     CDS             2289..2456
                     /locus_tag="IV038_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP8343221.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
BASE COUNT         1030 a          416 c          503 g          876 t
ORIGIN      
        1 aagataacat tcaaaaggag ataataaaaa tgcggcaagg tattcttaaa taaactgtca
       61 attagatagc gggaataatt aattaagcat ccttatttaa aataaggact ggttttttgt
      121 gcccagttta agaatacttt tatcatttta tcataaagca tctgtggcta tcagtgtgta
      181 gtatacagct atttttgtat taaaatccca gtgataagag tatttatcac tgggattttt
      241 tctgcttttt gagcttttga atggaggaaa taaaattgaa aattattaat atcggtatct
      301 tagcacatgt tgatgcagga aaaactactt tgacagaaag cttactatac agtagcggag
      361 caattaaaga gttaggaagt gtagatagcg gtacaacgaa aacggatact atgtttttgg
      421 aacgccagag aggtattact attcagaccg caataacatc ttttcaacgg gaaaatgtta
      481 aagtaaatat tgtagatact cctggacaca tggatttttt ggcagatgta taccgttcat
      541 tatctgtttt ggatggagct attttgctaa tctctgcaaa agatggagta cagtcacaaa
      601 ctcgtatact attccatgca cttagaaaga tgaacatacc tataatattt tttattaaca
      661 aaattgatca aaatggaata aatttgccag atgtttatca agatattaag gacaaacttt
      721 ctgacgacat cataattaag cagactgtga atctaaattt gaaaccttat gtaatagatt
      781 atactgaacc agaacaatgg gagacagtaa ttgtgggaaa tgattattta ttagaaaaat
      841 ataccattgg gaaaacattg aatattgcag aacttgaaaa ggaggaaaac gaaagaattc
      901 aaagttgctc cttatatcct gtttatcacg gaagtgcaaa gaataatatt ggaattaaac
      961 aacttataga ggtaattact agcaaattat tttcacccac acaactcaat tcagataaac
     1021 tttgtggaaa tgtttttaaa gtagaatatt cagatgatgg tcaacggctt gtctatgtac
     1081 gtctttatag tggaacgcta catttgcgag actcagtcaa tatatcagaa aaggaaaaaa
     1141 taaaagttac agaaatgtat acttcaataa atggagaatt acgccagata gataaggcag
     1201 agcctggtga gattattatt ttaaaaaatg agcttttaaa actaaataac gtacttggag
     1261 ataaaaaaag attaccacat agagaaattc ttgagaatcc tcttcctatg ttacaaacaa
     1321 caattgaacc atgtaaatca gtacaaagag aaaagttact agatgcactt tttgaaatat
     1381 ccgatagtga tccccttcta caatattatg tagatacagt aactcacgaa attgtgctat
     1441 cttttttagg tgaggtccaa atggaggtaa cttgtactct gattcaagaa aaatatcata
     1501 ttgagataga aacaagaaaa ccaactgtca tttatatgga aagaccatta aaaaaatctg
     1561 aatttaccat tgatatcgaa gtacctccaa atcctttctg ggcttctatt ggtttatctg
     1621 taacaccact tcctttgggt agtggcattc agtatgagag cctggtttct ctaggttatt
     1681 taaatcaatc atttcaaaat gcagttatgg aaggtatacg ctatgggtgt gaacaaggat
     1741 tgtacggttg gaaattaaca gactgtaaga tctgttttaa gtatggtcta tattacagcc
     1801 ctgtcagtac gccagcagat ttccgaatgc ttgcgcctat tgtactagag caggctttta
     1861 gaaagagtgg tacagagtta ttagagccat atcttagctt cgaaatttat gtaccacaag
     1921 aatatctttc gagagcatat aatgatgctt ccaaatattg tgcaaatatt ttaaatacta
     1981 agttaaaagg taacgaggtc attctcattg gtgaaattcc agcccgttgt attcaagagt
     2041 atcgaaacag tttaactttc tttacaaatg gacgcagtgt ctgtttaaca gagttaaaag
     2101 gttatcaggt tactaacatt aagtctgctt tccaaccacg tcgtccaaat aatagaatag
     2161 acaaagtaag gcatatgttt aataaaatca acttacattg atagtgaact aaagagaaat
     2221 ttttaaaatg aatattgagt tgttaattag aacaggagga tatagatatt gtaaatacta
     2281 ggtggatact gtgccctata tgtggcaata agacccgttt aaaaatgagg gaagatactg
     2341 aattaaaaaa tttccctcta ttttgtccta aatgcagaca agaaatttta attgaaataa
     2401 cgaagtttag aataactgta attacagagc cagacgcaaa gacgcagagc cgataattcg
     2461 agatatcaaa atctcatttt atcggctttt tcttttctat gcataccatt gagcttagtt
     2521 cttgatatcc tttataagaa aataagtgta ctgatttttt ttgcagtagc tgtattacag
     2581 tagacatccc tcaagcataa aaagtataca attaaaattc aatacttgat tattgatgaa
     2641 gtacagagtt actcgtcatt tcaaaactag tatatcagtc aagtttttcc taaggaacaa
     2701 aaacgaactt cgctatggga atcaaaaatt tttagtcgga tgaagtgata tgcaataaat
     2761 ccagaaaatt ttcccaacaa caaaaaatga actgtacttt gcagcgataa gagtaaccaa
     2821 agaac
//
LOCUS       DACDCJ010000272         2802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.173, whole genome shotgun sequence.
ACCESSION   DACDCJ010000272 DACDCJ010000000
VERSION     DACDCJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.173"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..340
                     /locus_tag="IV038_003110"
     CDS             <1..340
                     /locus_tag="IV038_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP8343222.1"
                     /translation="MEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQQLADLLKDKKK
                     LAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHGTDIDAACGQLR
                     SKQMKKTVAK"
     gene            complement(533..1636)
                     /locus_tag="IV038_003111"
     CDS             complement(533..1636)
                     /locus_tag="IV038_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP8343223.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            2189..2737
                     /gene="aac(6')"
                     /locus_tag="IV038_003112"
     CDS             2189..2737
                     /gene="aac(6')"
                     /locus_tag="IV038_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP8343224.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          924 a          483 c          544 g          851 t
ORIGIN      
        1 gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat
       61 catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa
      121 agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga
      181 tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa
      241 cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca
      301 attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt
      361 caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa
      421 aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa
      481 gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga
      541 ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg
      601 cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca
      661 tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg
      721 atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct
      781 gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct
      841 ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt
      901 tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt
      961 caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca
     1021 agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat
     1081 catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg
     1141 cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag
     1201 gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata
     1261 ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta
     1321 gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga
     1381 cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat
     1441 tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt
     1501 tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt
     1561 ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga
     1621 aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa
     1681 gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt
     1741 gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag
     1801 tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt
     1861 ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca
     1921 ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga
     1981 tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct attttgaata
     2041 aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat
     2101 actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta
     2161 gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc
     2221 agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga
     2281 cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt
     2341 agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg
     2401 ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt
     2461 agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac
     2521 ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga
     2581 taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg
     2641 ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat
     2701 ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt
     2761 aatcagcttc aagaaataag aaggaattta cgaaaattac tt
//
LOCUS       DACDCJ010000273         2551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31911_2016
            SAMN12640395-rid9893013.guided.179, whole genome shotgun sequence.
ACCESSION   DACDCJ010000273 DACDCJ010000000
VERSION     DACDCJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640395
            Sequence Read Archive: SRR10041156
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000743100.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000743100.1
            Genome Coverage       :: 69x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/22/2020 23:24:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,969
            CDSs (with protein)               :: 2,969
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 35 of 82
            Pseudo Genes (incomplete)         :: 44 of 82
            Pseudo Genes (internal stop)      :: 19 of 82
            Pseudo Genes (multiple problems)  :: 12 of 82
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640395-rid9893013.guided.179"
                     /isolate="E31911_2016"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2016"
                     /collected_by="Murdoch University"
     gene            <1..459
                     /locus_tag="IV038_003113"
     CDS             <1..459
                     /locus_tag="IV038_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP8343225.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR"
                     /locus_tag="IV038_003114"
     CDS             673..1368
                     /gene="vanR"
                     /locus_tag="IV038_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP8343226.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="IV038_003115"
     CDS             1346..2500
                     /locus_tag="IV038_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP8343227.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          880 a          453 c          535 g          683 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa t
//
